LOCUS       DAVLXF010000001       121968 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLXF010000001 DAVLXF010000000
VERSION     DAVLXF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 121968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 121968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..121968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.001"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            56..1948
                     /gene="pepO"
                     /locus_tag="VYO12_000001"
     CDS             56..1948
                     /gene="pepO"
                     /locus_tag="VYO12_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1586679.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVATVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2071..2700)
                     /locus_tag="VYO12_000002"
     CDS             complement(2071..2700)
                     /locus_tag="VYO12_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1586680.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3696..5918
                     /locus_tag="VYO12_000003"
     CDS             3696..5918
                     /locus_tag="VYO12_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1586681.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5948..6391
                     /locus_tag="VYO12_000004"
     CDS             5948..6391
                     /locus_tag="VYO12_000004"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1586682.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGVAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGANMQVELVNNGPVTIILDTKKR"
     gene            complement(6430..6845)
                     /locus_tag="VYO12_000005"
                     /pseudo
     CDS             complement(6430..6845)
                     /locus_tag="VYO12_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7204..7566
                     /locus_tag="VYO12_000006"
     CDS             7204..7566
                     /locus_tag="VYO12_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586683.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEEDVLILLSELQNFFLDVFQAKRIHRA
                     FCVRLCLKAQFLLSNNDS"
     gene            7747..8046
                     /locus_tag="VYO12_000007"
     CDS             7747..8046
                     /locus_tag="VYO12_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586684.1"
                     /translation="MNRDVLEFLRTETAEKISLYISEANRLEGDVTLLAPSSQDLEDI
                     KNAMLSNSNLGLKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(8141..8578)
                     /locus_tag="VYO12_000008"
     CDS             complement(8141..8578)
                     /locus_tag="VYO12_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586685.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(8680..9345)
                     /locus_tag="VYO12_000009"
     CDS             complement(8680..9345)
                     /locus_tag="VYO12_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV1586686.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVGISMLSTRF
                     TGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMNLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIEGNSN
                     KEY"
     gene            complement(9533..10183)
                     /locus_tag="VYO12_000010"
     CDS             complement(9533..10183)
                     /locus_tag="VYO12_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1586687.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            10295..11137
                     /locus_tag="VYO12_000011"
     CDS             10295..11137
                     /locus_tag="VYO12_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1586688.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQENFDRLVTHPPCDIAVY
                     GHIHQQLLRHGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDGKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            11213..11650
                     /locus_tag="VYO12_000012"
     CDS             11213..11650
                     /locus_tag="VYO12_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1586689.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            11719..12021
                     /locus_tag="VYO12_000013"
     CDS             11719..12021
                     /locus_tag="VYO12_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586690.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFTFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(12112..13185)
                     /locus_tag="VYO12_000014"
     CDS             complement(12112..13185)
                     /locus_tag="VYO12_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1586691.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            13341..14018
                     /locus_tag="VYO12_000015"
     CDS             13341..14018
                     /locus_tag="VYO12_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1586692.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            14011..14985
                     /locus_tag="VYO12_000016"
     CDS             14011..14985
                     /locus_tag="VYO12_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1586693.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLFLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(15034..16977)
                     /gene="thrS"
                     /locus_tag="VYO12_000017"
     CDS             complement(15034..16977)
                     /gene="thrS"
                     /locus_tag="VYO12_000017"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1586694.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNIAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVNYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(17035..17358)
                     /locus_tag="VYO12_000018"
     CDS             complement(17035..17358)
                     /locus_tag="VYO12_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1586695.1"
                     /translation="MELQELVEHSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            18223..18768
                     /locus_tag="VYO12_000019"
     CDS             18223..18768
                     /locus_tag="VYO12_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1586696.1"
                     /translation="MKLAVIAANGQVGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAES
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            18994..19263
                     /gene="rpsO"
                     /locus_tag="VYO12_000020"
     CDS             18994..19263
                     /gene="rpsO"
                     /locus_tag="VYO12_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1586697.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            20079..20693
                     /locus_tag="VYO12_000021"
     CDS             20079..20693
                     /locus_tag="VYO12_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1586698.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(20986..21735)
                     /locus_tag="VYO12_000022"
     CDS             complement(20986..21735)
                     /locus_tag="VYO12_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1586699.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMILKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            21868..24204
                     /locus_tag="VYO12_000023"
     CDS             21868..24204
                     /locus_tag="VYO12_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1586700.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            24305..24982
                     /locus_tag="VYO12_000024"
     CDS             24305..24982
                     /locus_tag="VYO12_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1586701.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVPHVSK"
     gene            24969..25766
                     /locus_tag="VYO12_000025"
     CDS             24969..25766
                     /locus_tag="VYO12_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1586702.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            25783..26886
                     /locus_tag="VYO12_000026"
     CDS             25783..26886
                     /locus_tag="VYO12_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1586703.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWLSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLSILDQAPANELFPGLQGFDNLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            26893..27084
                     /locus_tag="VYO12_000027"
     CDS             26893..27084
                     /locus_tag="VYO12_000027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586704.1"
                     /translation="MKIKEQTRKLAADYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            27187..28206
                     /gene="galE"
                     /locus_tag="VYO12_000028"
     CDS             27187..28206
                     /gene="galE"
                     /locus_tag="VYO12_000028"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1586705.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGIIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            28208..29173
                     /locus_tag="VYO12_000029"
     CDS             28208..29173
                     /locus_tag="VYO12_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1586706.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(29224..29430)
                     /locus_tag="VYO12_000030"
     CDS             complement(29224..29430)
                     /locus_tag="VYO12_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1586707.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            29482..29958
                     /locus_tag="VYO12_000031"
     CDS             29482..29958
                     /locus_tag="VYO12_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1586708.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            29967..30638
                     /locus_tag="VYO12_000032"
     CDS             29967..30638
                     /locus_tag="VYO12_000032"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1586709.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            30768..31106
                     /locus_tag="VYO12_000033"
     CDS             30768..31106
                     /locus_tag="VYO12_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1586710.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIHIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            31109..31738
                     /locus_tag="VYO12_000034"
     CDS             31109..31738
                     /locus_tag="VYO12_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1586711.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            31978..33129
                     /locus_tag="VYO12_000035"
     CDS             31978..33129
                     /locus_tag="VYO12_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1586712.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGARLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            33447..34196
                     /gene="truA"
                     /locus_tag="VYO12_000036"
     CDS             33447..34196
                     /gene="truA"
                     /locus_tag="VYO12_000036"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1586713.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQIDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            34186..34959
                     /locus_tag="VYO12_000037"
     CDS             34186..34959
                     /locus_tag="VYO12_000037"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1586714.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            34937..35398
                     /locus_tag="VYO12_000038"
     CDS             34937..35398
                     /locus_tag="VYO12_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1586715.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(35657..35860)
                     /locus_tag="VYO12_000039"
     CDS             complement(35657..35860)
                     /locus_tag="VYO12_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1586716.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(35853..36935)
                     /locus_tag="VYO12_000040"
     CDS             complement(35853..36935)
                     /locus_tag="VYO12_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1586717.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(37061..37843)
                     /locus_tag="VYO12_000041"
     CDS             complement(37061..37843)
                     /locus_tag="VYO12_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1586718.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(37843..39186)
                     /locus_tag="VYO12_000042"
     CDS             complement(37843..39186)
                     /locus_tag="VYO12_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1586719.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGTYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDVQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(39285..40601)
                     /locus_tag="VYO12_000043"
     CDS             complement(39285..40601)
                     /locus_tag="VYO12_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1586720.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQNLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDINGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            40794..42020
                     /locus_tag="VYO12_000044"
     CDS             40794..42020
                     /locus_tag="VYO12_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1586721.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYILWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            42219..43793
                     /locus_tag="VYO12_000045"
     CDS             42219..43793
                     /locus_tag="VYO12_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1586722.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            44057..44845
                     /gene="codY"
                     /locus_tag="VYO12_000046"
     CDS             44057..44845
                     /gene="codY"
                     /locus_tag="VYO12_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1586723.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGQLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            44845..45420
                     /locus_tag="VYO12_000047"
     CDS             44845..45420
                     /locus_tag="VYO12_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1586724.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            45502..45587
                     /locus_tag="VYO12_000048"
     tRNA            45502..45587
                     /locus_tag="VYO12_000048"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45536..45538,aa:Leu,seq:aag)
     gene            45870..47000
                     /gene="ugpC"
                     /locus_tag="VYO12_000049"
     CDS             45870..47000
                     /gene="ugpC"
                     /locus_tag="VYO12_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1586725.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(47122..47292)
                     /locus_tag="VYO12_000050"
     CDS             complement(47122..47292)
                     /locus_tag="VYO12_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586726.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEYKEHHNIDA"
     gene            47499..48257
                     /locus_tag="VYO12_000051"
                     /pseudo
     CDS             47499..48257
                     /locus_tag="VYO12_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            48345..48857
                     /locus_tag="VYO12_000052"
     CDS             48345..48857
                     /locus_tag="VYO12_000052"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1586727.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            49039..49983
                     /locus_tag="VYO12_000053"
     CDS             49039..49983
                     /locus_tag="VYO12_000053"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1586728.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            49992..50669
                     /locus_tag="VYO12_000054"
     CDS             49992..50669
                     /locus_tag="VYO12_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1586729.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            50767..51525
                     /locus_tag="VYO12_000055"
     CDS             50767..51525
                     /locus_tag="VYO12_000055"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1586730.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            51563..53068
                     /gene="lytC"
                     /locus_tag="VYO12_000056"
     CDS             51563..53068
                     /gene="lytC"
                     /locus_tag="VYO12_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1586731.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRNGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            53564..54082
                     /locus_tag="VYO12_000057"
     CDS             53564..54082
                     /locus_tag="VYO12_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1586732.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            54212..54718
                     /locus_tag="VYO12_000058"
     CDS             54212..54718
                     /locus_tag="VYO12_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1586733.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            54718..54888
                     /locus_tag="VYO12_000059"
     CDS             54718..54888
                     /locus_tag="VYO12_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586734.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            54920..56152
                     /gene="clpX"
                     /locus_tag="VYO12_000060"
     CDS             54920..56152
                     /gene="clpX"
                     /locus_tag="VYO12_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1586735.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            56161..56748
                     /locus_tag="VYO12_000061"
     CDS             56161..56748
                     /locus_tag="VYO12_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1586736.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            56759..57139
                     /locus_tag="VYO12_000062"
     CDS             56759..57139
                     /locus_tag="VYO12_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1586737.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            57191..58081
                     /gene="rapZ"
                     /locus_tag="VYO12_000063"
     CDS             57191..58081
                     /gene="rapZ"
                     /locus_tag="VYO12_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1586738.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            58078..59055
                     /locus_tag="VYO12_000064"
     CDS             58078..59055
                     /locus_tag="VYO12_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1586739.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDFRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            59052..59963
                     /gene="whiA"
                     /locus_tag="VYO12_000065"
     CDS             59052..59963
                     /gene="whiA"
                     /locus_tag="VYO12_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1586740.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(59997..60965)
                     /locus_tag="VYO12_000066"
     CDS             complement(59997..60965)
                     /locus_tag="VYO12_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1586741.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELESVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            61101..61958
                     /locus_tag="VYO12_000067"
     CDS             61101..61958
                     /locus_tag="VYO12_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1586742.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            61945..62724
                     /locus_tag="VYO12_000068"
     CDS             61945..62724
                     /locus_tag="VYO12_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1586743.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            62748..64100
                     /locus_tag="VYO12_000069"
     CDS             62748..64100
                     /locus_tag="VYO12_000069"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1586744.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGNIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            64174..64548
                     /locus_tag="VYO12_000070"
     CDS             64174..64548
                     /locus_tag="VYO12_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1586745.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            64550..65398
                     /locus_tag="VYO12_000071"
     CDS             64550..65398
                     /locus_tag="VYO12_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1586746.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            65675..66442
                     /locus_tag="VYO12_000072"
     CDS             65675..66442
                     /locus_tag="VYO12_000072"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1586747.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMHIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            66454..67638
                     /locus_tag="VYO12_000073"
     CDS             66454..67638
                     /locus_tag="VYO12_000073"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1586748.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHNPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            67635..69506
                     /locus_tag="VYO12_000074"
     CDS             67635..69506
                     /locus_tag="VYO12_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1586749.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQAYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(69649..70833)
                     /gene="mntE"
                     /locus_tag="VYO12_000075"
     CDS             complement(69649..70833)
                     /gene="mntE"
                     /locus_tag="VYO12_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1586750.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLATGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            71137..73833
                     /locus_tag="VYO12_000076"
     CDS             71137..73833
                     /locus_tag="VYO12_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1586751.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVDLATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            73846..74637
                     /gene="yghU"
                     /locus_tag="VYO12_000077"
     CDS             73846..74637
                     /gene="yghU"
                     /locus_tag="VYO12_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1586752.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRQTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QENLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            74753..75163
                     /locus_tag="VYO12_000078"
     CDS             74753..75163
                     /locus_tag="VYO12_000078"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1586753.1"
                     /translation="MEKKIVKDNLFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            75175..76770
                     /locus_tag="VYO12_000079"
     CDS             75175..76770
                     /locus_tag="VYO12_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586754.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            76937..77101
                     /locus_tag="VYO12_000080"
     CDS             76937..77101
                     /locus_tag="VYO12_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1586755.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            77180..77908
                     /gene="yaaA"
                     /locus_tag="VYO12_000081"
     CDS             77180..77908
                     /gene="yaaA"
                     /locus_tag="VYO12_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1586756.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(78186..78734)
                     /locus_tag="VYO12_000082"
     CDS             complement(78186..78734)
                     /locus_tag="VYO12_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1586757.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALASKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            79055..79564
                     /locus_tag="VYO12_000083"
     CDS             79055..79564
                     /locus_tag="VYO12_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1586758.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GLWNYPTVF"
     gene            79540..80727
                     /locus_tag="VYO12_000084"
     CDS             79540..80727
                     /locus_tag="VYO12_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1586759.1"
                     /translation="MELSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            80720..81085
                     /locus_tag="VYO12_000085"
     CDS             80720..81085
                     /locus_tag="VYO12_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1586760.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            81101..82447
                     /gene="asnS"
                     /locus_tag="VYO12_000086"
     CDS             81101..82447
                     /gene="asnS"
                     /locus_tag="VYO12_000086"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1586761.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            82600..82890
                     /locus_tag="VYO12_000087"
     CDS             82600..82890
                     /locus_tag="VYO12_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1586762.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            82902..83372
                     /gene="ssbA"
                     /locus_tag="VYO12_000088"
     CDS             82902..83372
                     /gene="ssbA"
                     /locus_tag="VYO12_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1586763.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            83404..83643
                     /locus_tag="VYO12_000089"
     CDS             83404..83643
                     /locus_tag="VYO12_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1586764.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            83938..85338
                     /locus_tag="VYO12_000090"
     CDS             83938..85338
                     /locus_tag="VYO12_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1586765.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            85340..85699
                     /locus_tag="VYO12_000091"
     CDS             85340..85699
                     /locus_tag="VYO12_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1586766.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            85753..86502
                     /locus_tag="VYO12_000092"
     CDS             85753..86502
                     /locus_tag="VYO12_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1586767.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            86492..86791
                     /locus_tag="VYO12_000093"
     CDS             86492..86791
                     /locus_tag="VYO12_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1586768.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            86862..87797
                     /locus_tag="VYO12_000094"
     CDS             86862..87797
                     /locus_tag="VYO12_000094"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1586769.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            87883..88395
                     /locus_tag="VYO12_000095"
     CDS             87883..88395
                     /locus_tag="VYO12_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1586770.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            88420..89028
                     /locus_tag="VYO12_000096"
     CDS             88420..89028
                     /locus_tag="VYO12_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1586771.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            89129..89326
                     /locus_tag="VYO12_000097"
     CDS             89129..89326
                     /locus_tag="VYO12_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1586772.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(89369..90814)
                     /locus_tag="VYO12_000098"
     CDS             complement(89369..90814)
                     /locus_tag="VYO12_000098"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1586773.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            90920..92542
                     /locus_tag="VYO12_000099"
     CDS             90920..92542
                     /locus_tag="VYO12_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1586774.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEDSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            92745..94703
                     /locus_tag="VYO12_000100"
     CDS             92745..94703
                     /locus_tag="VYO12_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1586775.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            94875..95420
                     /locus_tag="VYO12_000101"
     CDS             94875..95420
                     /locus_tag="VYO12_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1586776.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDMSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEI"
     gene            95429..95968
                     /locus_tag="VYO12_000102"
     CDS             95429..95968
                     /locus_tag="VYO12_000102"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1586777.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETSFALLFIFATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKLLLLDHLDQENQRLCVC"
     gene            96004..96549
                     /locus_tag="VYO12_000103"
     CDS             96004..96549
                     /locus_tag="VYO12_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1586778.1"
                     /translation="MEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLV
                     TDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEILI
                     FDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSGT
                     HKELLENHERYARFVQEQMIE"
     gene            96706..97800
                     /locus_tag="VYO12_000104"
     CDS             96706..97800
                     /locus_tag="VYO12_000104"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1586779.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            97808..98974
                     /locus_tag="VYO12_000105"
     CDS             97808..98974
                     /locus_tag="VYO12_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1586780.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            99067..102165
                     /locus_tag="VYO12_000106"
     CDS             99067..102165
                     /locus_tag="VYO12_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1586781.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGLTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            102213..102830
                     /locus_tag="VYO12_000107"
     CDS             102213..102830
                     /locus_tag="VYO12_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1586782.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            102841..104175
                     /locus_tag="VYO12_000108"
     CDS             102841..104175
                     /locus_tag="VYO12_000108"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1586783.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            104185..104649
                     /locus_tag="VYO12_000109"
     CDS             104185..104649
                     /locus_tag="VYO12_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1586784.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEEILLYATSQGYSMIWFNPFYLEEQ"
     gene            104646..105140
                     /locus_tag="VYO12_000110"
     CDS             104646..105140
                     /locus_tag="VYO12_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1586785.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            105221..106876
                     /gene="mltG"
                     /locus_tag="VYO12_000111"
     CDS             105221..106876
                     /gene="mltG"
                     /locus_tag="VYO12_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1586786.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            106947..107429
                     /gene="greA"
                     /locus_tag="VYO12_000112"
     CDS             106947..107429
                     /gene="greA"
                     /locus_tag="VYO12_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1586787.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            107757..108155
                     /locus_tag="VYO12_000113"
     CDS             107757..108155
                     /locus_tag="VYO12_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1586788.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            108600..109607
                     /locus_tag="VYO12_000114"
                     /pseudo
     CDS             108600..109607
                     /locus_tag="VYO12_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            109682..109882
                     /locus_tag="VYO12_000115"
     CDS             109682..109882
                     /locus_tag="VYO12_000115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1586789.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            109917..110633
                     /gene="atpB"
                     /locus_tag="VYO12_000116"
     CDS             109917..110633
                     /gene="atpB"
                     /locus_tag="VYO12_000116"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1586790.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQTIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            110647..111141
                     /gene="atpF"
                     /locus_tag="VYO12_000117"
     CDS             110647..111141
                     /gene="atpF"
                     /locus_tag="VYO12_000117"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1586791.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            111141..111677
                     /locus_tag="VYO12_000118"
     CDS             111141..111677
                     /locus_tag="VYO12_000118"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1586792.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            111692..113197
                     /locus_tag="VYO12_000119"
     CDS             111692..113197
                     /locus_tag="VYO12_000119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1586793.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            113213..114091
                     /locus_tag="VYO12_000120"
     CDS             113213..114091
                     /locus_tag="VYO12_000120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1586794.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            114177..115583
                     /gene="atpD"
                     /locus_tag="VYO12_000121"
     CDS             114177..115583
                     /gene="atpD"
                     /locus_tag="VYO12_000121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1586795.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            115594..116013
                     /locus_tag="VYO12_000122"
     CDS             115594..116013
                     /locus_tag="VYO12_000122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1586796.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(116058..117071)
                     /locus_tag="VYO12_000123"
     CDS             complement(116058..117071)
                     /locus_tag="VYO12_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1586797.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            117215..118381
                     /locus_tag="VYO12_000124"
     CDS             117215..118381
                     /locus_tag="VYO12_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1586798.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            118371..119600
                     /locus_tag="VYO12_000125"
     CDS             118371..119600
                     /locus_tag="VYO12_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1586799.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            119746..120387
                     /locus_tag="VYO12_000126"
     CDS             119746..120387
                     /locus_tag="VYO12_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1586800.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            120397..121026
                     /locus_tag="VYO12_000127"
     CDS             120397..121026
                     /locus_tag="VYO12_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1586801.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASELLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            121039..121875
                     /locus_tag="VYO12_000128"
     CDS             121039..121875
                     /locus_tag="VYO12_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1586802.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
BASE COUNT        35939 a        22659 c        27654 g        35716 t
ORIGIN      
        1 ttccctagtg atttttgtta agataaatgc aaatacaaat gaaagcgaga acaagatgac
       61 acgttatcaa gatgattttt atgatgctat caatggagaa tggcaacaga cagctgaaat
      121 cccagcagat aagtctcaaa ctggaggttt tgttgattta gaccaggaaa ttgaagacct
      181 gatgctggcg acaacagaca agtggttagc aggtgaagaa gtgcctgagg atgctatctt
      241 ggaaaacttt gtcaaatacc accgcctagt tcgtgatttt gacaagagag aagctgacgg
      301 tatcacacct gtcttaccac tccttaaaga attccaagaa ttggaaactt ttgcggattt
      361 tacagctaaa ctagcagagt ttgagcttgc aggaaaacca aacttccttc cttttggtgt
      421 atcgccagac tttatggatg ctagaatcaa tgttctatgg gctagcgctc caagcacaat
      481 cttgccagat acgacctact atgcagaaga acatcctcag cgcgaagagc tcttgactct
      541 ttggaaagaa agcagcgcaa atctcctcaa ggcttatgat ttctctgatg aagaaattga
      601 agacttgcta gaaaaaagac ttgaattgga ccgccgagtt gcgacagtgg tgctctctaa
      661 tgaagaaagt tcagaatatg ctaaactcta tcatccatat tcttacgaag atttcaagaa
      721 attcgcgcct gccctacctt tggatgactt cttcaaagca gttattgggc aattaccaga
      781 caaggttatt gtagacgagg aacgtttctg gcaagcagca gagcaattct acagtgagga
      841 agcctggtct ctccttaaag caaccttgat tttgagtgtt gtcaatcttt caaccagcta
      901 tttaacagag gatatccgtg ttttgtctgg tgcctacagc cgtgcccttt ctggagttcc
      961 agaggcaaaa gataaggtca aagcagctta tcatctagca caggaacctt tcaagcaagc
     1021 cctggggctt tggtacgccc gtgagaagtt ctctccagaa gccaaggcgg atgtggagaa
     1081 aaaagtggca accatgattg atgtctataa ggagcgtctg cttaagaatg actggctcac
     1141 tccagaaacc tgtaaacagg ctatcgtgaa gctcaatgtg atcaaacctt atattggcta
     1201 tccagaagaa ttgcctgcac gttacaagga taaggtagtg aatgaaactg ccagtctttt
     1261 tgagaatgct ctagcctttg cgcgtgtgga aatcaagcac agttggagta agtggaacca
     1321 gcctgtagac tataaggaat ggggcatgcc tgctcatatg gtcaatgcct actacaatcc
     1381 tcagaagaac ctgattgtct ttccagcggc cattttacag gcgcctttct atgacttgca
     1441 tcagtcatct tctgctaact acggtggtat tggggcagtg attgcccatg aaatttccca
     1501 cgcctttgat actaacgggg cttcctttga cgaaaatggt agcctcaagg attggtggac
     1561 agagagcgac tatgctgcct tcaaggagaa aacacaaaaa gtcattgacc aatttgatgg
     1621 acaggattct tatggagcaa ccattaacgg taaattgact gtatcagaaa acgtggctga
     1681 cttgggagga atcgcagcag cgcttgaagc agctaagaga gaagcagact tctcagcaga
     1741 agagttcttc tacaacttcg gtcgcatctg gcgcatgaaa ggtcgtccag aatttatgaa
     1801 acttttggct agcgtcgatg tgcacgcacc agccaaactc cgtgtcaatg tgcaagtacc
     1861 aaacttcgac gatttcttta caacctatga tgtcaaagaa ggagacggaa tgtggcgttc
     1921 accagaggag cgcgtgatta tttggtaata caaaaaatct agtcatcaga aaaaaggcat
     1981 ccaatcacgt gtgaaaacct tgataggatg ccttttgtaa tagaaagatt tgctggatag
     2041 tttacttaaa tttcgattgt catcatcttg ctagaaaaag ggattgaagg ccttttcgtg
     2101 agcgatagta gtagctggac catgtcctgg atagacatcg tagtttggta gggtgaagag
     2161 ttgggtctgg atactatgaa ggagttgctc catgctacca gtcggaaggt cggtccgtcc
     2221 gatagtttcg cggaatagag catctcccgt caagactagg tgagcatcag gaaagactag
     2281 ggaaacacca ccgatagagt gccctggggt cggtagaacc ttaaaacgaa attcctctag
     2341 ttggtattct tcgtgaaaga caaaggtgtg ttctgcaggt tttgtgacca catctgccat
     2401 atcatcgtgg cgagggagac cggagagatt atcgacagga gtgtagagcc agctggcttc
     2461 gctctctgcg atatagacag gaggattgcc aaacgtctcg cgaaccaagt ccagactcat
     2521 gatatggtca taatgggcgt gggtcaagag aatagcacag atcggtttgt tgatcttctc
     2581 gattgtctga cgaatggctt cccaatgact accaggatcg acgacgatga ggtgcttttc
     2641 gccttctaga taataggtat tttcataggc aacaggattc acggttttat ggattttcat
     2701 actagctcca atctcaaaga atgtactaat actcttcgaa aatctcttca aaccgcgtca
     2761 actttatctg caacctcaaa gcagtgcttt gaacagcctg cggctagctt tctagtttgc
     2821 tctttgattt tcattgagta taaattaagt atagcacagt tagggagaat aggtaaggat
     2881 ttaaaatgaa cttaaaaacc agcatgacta gatgaagaca ggctggttat caatttatca
     2941 atattggaaa ggtgtcaatt gcccttcata ggttgcataa actccgtcat tagcttgttt
     3001 gatagacgtg atgtttagag tgctaccgta gttggcttct catttgtcgc tatattcact
     3061 ataagttatc ctactgggca ggttctggtc gttagcatag tattgaacga ctgtttttta
     3121 tagttttgct ggctgaatag gaacaggcag caaactccta acattaggag agcaaagata
     3181 gaaatagcag tttttttcat aggtattctc cttataaact aaaggataga agaaagtatc
     3241 ttgaggattt ttatactctt caaaaatctc ttcaaaccgc gtcaacgtcg ccttgccgta
     3301 tatatgtgac tgactttgtc agtcttatct acaacctcaa agcagtgctt tgaacagcct
     3361 gcggctagtt tcctagtttg ctcttggatt ttcattgagt attaattcca aaatccagtc
     3421 aaggtaccag tgtatgtgat acgagttcca ccgtttatct ttcgtatctt caatggaatt
     3481 gggaaaatca gaaagtcttt gggttacaat atggtttccg ttcttctatc tctccttcta
     3541 tcacctaagt ttaaaatcta acttcgtagt atattatggg ccaataattc agaattatca
     3601 agcaattttg aattattttt ttcttattct gttccaatct agttttcata ttcttaaaaa
     3661 agtgataaaa tggtaggaag aattggagag tagagatgcc gaaagaagtg aatttaacag
     3721 gcgaagaagt tgtcgcttta accaaagaat atttaacgga agaggatgtt cattttgtcc
     3781 ataaggcctt ggtctatgct gttgaatgcc acagtggtca atatcgcaaa tcaggcgagc
     3841 cttatatcat tcaccctatc caagtggcag gtattttagc taagctaaag ctggatgctg
     3901 taacagtagc ttgtggattc ttgcatgatg tggtggaaga tacagatgcg accttggacg
     3961 atttggaaag agagtttggt cctgatgtgc gggtgattgt tgacggagtt accaagcttg
     4021 gcaaggtcga gtacaaatcg atcgaggagc aattagcgga aaatcatcgc aagatgctca
     4081 tggccatgtc tgaggacatc cgcgttattt tggtcaaact gtctgaccgc ttgcacaata
     4141 tgcggaccct gaaacatctt cgaaaagaca agcaggagcg tatttccaaa gaaaccatgg
     4201 aaatctatgc cccacttgcc catcgtttgg ggatttccag tgtcaaatgg gaattagaag
     4261 acttgtcttt ccgttatctc aatccaacgg agttttacaa gattacccat atgatgaagg
     4321 aaaagcgcag ggagcgtgag gccttggtgg atgaggtagt cacaaaatta gaggagtata
     4381 cgacagaacg tcacttgaaa gggaagattt atggtcgtcc caagcatatt tactcaattt
     4441 tccgcaaaat gcaggacaag agaaaacggt ttgaggaaat ctatgatctg attgctattc
     4501 gttgtatttt agatacccaa agtgatgttt atgccatgct tggttacgtg catgaatttt
     4561 ggaaaccgat gccaggtcgc ttcaaagact atattgccaa ccgcaaggcc aatggttatc
     4621 agtctatcca tacgactgtt tatggaccaa aagggccgat tgaattccag attcgaacca
     4681 aggaaatgca cgaggtggct gagtacgggg ttgcggctca ctgggcttat aagaaaggta
     4741 taaaggggca agttaacagc aaggaatcag ctattggaat gaactggatc aaggagatga
     4801 tggagctcca agaccaggct gatgatgcta aggaatttgt ggactctgtt aaggaaaact
     4861 atttggctga ggagatttac gtttttaccc cagatggagc tgtccgttcc cttcccaaag
     4921 attcaggacc gattgatttt gcctacgaaa tccataccaa ggtcggtgaa aaagcaactg
     4981 gtgccaaggt caatggccgc atggttccac tgacaaccaa gttaaagaca ggggatcagg
     5041 ttgaaattat cgccaacccg aactcctttg gacctagccg tgactggctc aatatggtca
     5101 agactagcaa ggcgcgcaat aagattcgcc agttctttaa aaaccaagat aaggaattgt
     5161 ctgtcaacaa gggtcgtgag atgctgatgg ctcagttcca agaaaatggc tatgtggcaa
     5221 ataaatttat ggacaagcgc cacatggatc aagttctgca aaagaccagt tacaagacag
     5281 aagactccct ctttgcggcc attggttttg gggaaatcgg tgcgattacc gtctttaacc
     5341 gtctgactga aaaggagcgc cgtgaggaag agcgtgccaa ggccaaggct gaggcagagg
     5401 agcttgtcaa aggtggcgag gtcaaggttg aaaataaaga aactctcaag gtcaagcatg
     5461 aggggggagt ggttattgaa ggtgcttctg gtctcctagt gcggattgct aagtgttgta
     5521 accccgtgcc tggtgacgat attgttggct acattaccaa gggtcgtggt gtggctattc
     5581 accgtgtgga ctgtatgaac ctgcgtgccc aagaaaacta cgagcaacgt ctccttgatg
     5641 tggaatggga agaccagtac tctagctcaa ataaggagta tatggcccat atcgatatct
     5701 acggtctcaa ccgtacagga ctgttgaacg atgtactgca agttctttca aatacaacca
     5761 agaatatttc aacggtcaat gcccaaccaa ccaaggatat gaagtttgct aatatccatg
     5821 tgtccttcgg tattgccaac ctctctacac tgactacggt tgtcgataaa attaagagtg
     5881 tgccagaagt ttactctgtc aaacggacca acggctagtt gtcctagctc ttactagaaa
     5941 ggctattatg aaaatcatta tccaacgggt taaaaaagcc caagtgagta tagaaggcca
     6001 gattcaggga aaaatcaatc agggactttt attgctggtt ggtgttggac cagaggacca
     6061 agaggaagat ttggactatg ctgtgagaaa actggtcaat atgcgaattt tttcagacgc
     6121 agaaggcaag atgaacctat ctgtcaaaga tattgaagga gaaatcctct ctatttctca
     6181 gtttaccctc tttgcggata ctaagaaagg caatcgtcca gcctttacag gggtagctaa
     6241 acctgatatg gcatcagact tctatgatgc tttcaatcaa aaattagcgc aagaagtgcc
     6301 cgttcagaca ggtatctttg gagcaaatat gcaggttgag ctggttaata acggacctgt
     6361 taccattatc ctagatacta aaaagagata agaaagacca agcccagtcg gcttggtctt
     6421 tctcatctat cataaaatac tccaaaaaga aatcggttct tgatatgctt gggggaagtc
     6481 tcttttcagg ctttggcaga tgcgatagga agggatgaga tgtcctagga tgaggagagt
     6541 tccctgaagg aaaagtggaa tatcacttaa agacaccaag gagagaatca tcaggctatc
     6601 ccataggaga atttgtaaag caaaacgcca gaatctcggt ctaatctggg aatagagttg
     6661 actaaagtgg tcacaaagtt ggtttgaaag ataggtcaat agcacaggat gaaagaaaat
     6721 gagccaaccg ctataaatca aaatccagtc ccaagtaagc cccccttttt aaatgtcaaa
     6781 aatgccagca tgattccatc aatgacaagg agttgaatgg ttttgatgaa gatgattttt
     6841 ttcatgctaa aacctccttt tccttagaga cactccccta caggtatttt agaatgtata
     6901 ttgcttagat attccttatt ttattagctt tttaaggtat taaagtagtt atataagttt
     6961 atgaagggta tcctatcctg cacggctaat tttgagttcg aaactgagga gtttataact
     7021 caaaatttcg attaaatcag catttaacga tagtatgttt gtcataaaat gataaatttt
     7081 tagattgtgg aaattttttt taacttaatt cttgattttt tttctaaatg tgatatactt
     7141 aatttacggg ataaaaaaac caccttagaa aggtgtgata atgtgcaaaa agagaatgtt
     7201 agaatgcgtt tagcgtctga atccagacag atattagata agcttaaaga tgatgtttac
     7261 aataaattag gctatgaagt aagctattcc agtattgtta gtcaagctgt tagagaatac
     7321 gttcctaaaa aagaacgtat agattggata aaattaaaag agacagctat acccttttca
     7381 tcgttaaaac agagcaataa ttgggagtac cagacatcac ttatgttaga ggaagatgta
     7441 ttaatcttac tgtctgaact acaaaatttt ttcttagatg tcttccaagc gaaacgtata
     7501 catagggcat tttgtgtacg cctatgcttg aaagcacaat ttttattaag taataatgat
     7561 tcttaatact atcaatatta atgcggagtg taatgctccg ctaaaataaa acctattacg
     7621 ggataaaaac actactttat ttaggatgga aagaggaacg aatgaagatg aaagctagag
     7681 attcaccatt gaattgattt tgtttttaga aaaaatcaat aattagaaaa aatacgaggt
     7741 aaaaaaatga atagagatgt attagagttt ctgagaacag agaccgctga aaaaattagt
     7801 ctttatataa gcgaggctaa tagattggag ggcgatgtaa ctctactcgc acctagttct
     7861 caagatttgg aagatattaa aaatgctatg ctttccaatt ctaatctcgg attaaaagtt
     7921 gcgagactgg atgtgatgaa gaaaatagca tatgcttcta caagaaatca ttatttaaca
     7981 ggggctacga tttttgggga tatttcgaaa ggaacataca attgtgatcc taaaagttat
     8041 gtctaatata agggaggtta atccctcccc ttgtttttga aaatcgaaag gaaaagtata
     8101 aaaatgaaag ttaatgaata tttctggaga acgagctgtc ttatactaaa aaagaagaaa
     8161 tcgataaaat agtatacgtg ttagagatgt gtttgtcgta aaagtctgga tagttttctc
     8221 ctaggtagtc agctgtcata taataaaaag tagaatggca agcagtgata attggcataa
     8281 gagatgaaaa acgctgagaa ctaatgaagg ctaagagaac aaaaaataga aaatcaatgg
     8341 tgagaacccc taaatagtag aagcgaatct ttttattgat ttgaggataa atctcagcga
     8401 attgatgttt gagtcggacg accaagagaa atagcaggag tccttgagca aggaagaaaa
     8461 ttaaaccaga gagcaagagc cactcaaaag atgtctcgag tctagtgata aaaaatgcaa
     8521 atagtagcaa atcaataact gcaattgcta taaaatggat tctatagagt tttttcatga
     8581 caagaccttc ctcttttatc taacttcatt ctactccaaa agaatgggag ttacaactaa
     8641 aatgataaaa atagcagaag ggagattctc ttaagttggc tagtattctt tatttgagtt
     8701 tccttctatg tatctaactt cttcatcatt ccagacaaat aaagctccga ttgcattgag
     8761 gatataaaag atgtatttac cgatattggc gaagtttcct tgaataccag cttttgtcag
     8821 ctgaacgaaa ttgtaaatca accaaaagcc ccactgagtt gttagtttca atgcattcaa
     8881 agcattggca atgagggaca gtgcaaaggc aatagttgtt acgtaggcaa ggagattcat
     8941 cttgccctca tatccgatat agttggtcac aaaggcaaag aggaaggcga tgatggaaat
     9001 gatgatggcc gccaatttta cctgtttttg gctcatttgg ttgggtctgc cttcttgcga
     9061 agctttccat ttctttatag caaaggtata aatgaggaag gtgacgggat aggtaatgat
     9121 ggccgcctta tttccaagga tataatcaat agcaccggac aaaatggtat taacaatacc
     9181 aaagtaattt ccccatttgc ttaatttccc cgtgaaacga gtggacaaca tggaaatccc
     9241 aacgttggtt acggaaatca atccaaaggg tacaagagct gtccatggtc cccagtctac
     9301 aaatttatcg aggcgtgagt tgaggtaacc agatgcaatc gcaatccaac gaccaaagca
     9361 accccgaaga ggtcaaacta tttagatgta gcaaaaattt ttagtgattt tttcataggt
     9421 taaactacct ttcttttttt caaatattct cccaccaaat gaaagtaaaa taaaatgata
     9481 gaaataaaac cctgaaaata aaactttcag ggttggtagg ggacttgaga aattagttga
     9541 ttttctcgac atagagttgt tttgcaatgt ccatattcac ttgtaaatcc tcgtcgttac
     9601 tgaggatagt gaaggtattg ctgaagccat caaactgctt gacttggagc tggtcaccga
     9661 tgtgaagtga gtgcttgtcc agataatgga gaatgtcaaa actatcgtgc acccgagtca
     9721 ggcggtaggc gccagcttcc ttgatatcag ctagtgggag gttattgatt tcaacgagga
     9781 gttctccctt ggcaggaata gttcctccgt gggggcaggt tttagggaaa cctagcagtt
     9841 tatctagtct ttccacgaac aggtcagaga cagtgtgttc caagacctca gcttcctcgt
     9901 gaatctggtc acttgtatag tctaaatgat gaactagaaa aacttcaatc aagcggtgct
     9961 tacgatagag ctcagagacc agtttgagac cgaggtcagt cagtagatag ccacattcct
    10021 tgtcctttag gatgagattt tcacttttca ttcgtttgat catttcagtt acggcagggg
    10081 gagagacttg catgcgagcc gcaatttcct tgttggtaat cttatgcagg tctatgccaa
    10141 tttcataaat acattttaga tagtcttctt tgtttggggt cattcgtttc ctcttgtcta
    10201 atatctttat ctagtatatc aaaaaaagct agatttctct agctgtgact ttttatgtta
    10261 tacttattgc aagagaaaaa acgaggagat ggatatgacg aaaatagctc ttctttcaga
    10321 tattcatgga aataccaccg ccttggaggc tgttttggca gatgctcagc agctaggagt
    10381 ggatgaatac tggcttttgg gagatattct catgccaggg acaggacgta gaaggatttt
    10441 ggacttgttg gatcaactac cgattacggc tagagttttg ggaaactggg aagacagtct
    10501 ttggcatggt gtccgtaagg aattggatag tactcgccct agtcaacgct atctcttgcg
    10561 ccagtgccag tatgttttag aggaaatttc cctagaagaa attgaagtgc tccacaatca
    10621 acctcttcaa attcatcgtc agtttgggga tttgacagtg ggaattagcc atcatctgcc
    10681 tgataagaac tgggggcgag agttgattca tactggcaaa caagagaatt ttgaccgctt
    10741 ggtgactcat cctccctgtg atattgctgt ttatggacat attcaccagc agttgcttcg
    10801 tcacgggact ggtgggcaat tgattgtcaa tccgggttcg attggccaac ctttcttttt
    10861 ggacgctcag ctacggaagg acttgcgggc ccagtatatg attttggagt ttgatggcaa
    10921 gggcctggta gatatggact tccgacgggt agactacgat gtggcagctg aattgcagct
    10981 ggctaaagac ctgagacttc cctattttga ggtttactat gaaagtctgg tcaatgggat
    11041 ccaccatact catcatcagg aatttttgag agaattggct cagaaagagg gttgcgatcg
    11101 ggagttagac gactggttga aaagtggtaa cgattgacat tacaactaaa aagggtataa
    11161 taatagaaaa agaagagtag aggcagggta gcctcgtaaa aaggagaaga ggatgcaaat
    11221 tccaagtaga tttaccattg cgactcatat gctgataatc attgccctcg aggggaagga
    11281 aagcaaggtg accagtgatt ttctggctgc tagtgtcggg gtcaatcctg tcattatcag
    11341 aaagatcttg tcccagttga agaaggcaga gctgatttca gtagcgcgtg gaacgggcgg
    11401 aacagagatt gtcaaggacc ttaaggatat tagtctttta gatgtttatc aggcggtcga
    11461 atgtcttggt aagacaggtc aactcttcag tttccatgac aatccgaatc caaattgccc
    11521 tgtaggagct catattcatg atgttttgga tcaaaaattg gagagaattc agttgactat
    11581 ggaggcagaa cttggtcaga ccagtctaga aaaagtcgtg gccgatgcag agagtcagat
    11641 gaaggattaa gagggataaa tgccctcttt ttttctttgg ataattatga taaaatgtag
    11701 ctattaaagg agtaagaaat gtatctcgtt atagaaattg tattagcctt tatagtgtca
    11761 ttttggaagg ataatagaag tttgtggaat ccagtattat ttttattatc cctcatttca
    11821 agttattttt atctatccta ccttttttat aaaaatggat acgagaatgt tcagttagct
    11881 ttttatgtat ttacctttgt cttacttcct tttttgcttt ttctaagtgg catcttttta
    11941 atctataatg gggtcatttt gttgaaacga gagggacgtt ccaagcccca ttatctctca
    12001 atgctctttg atttttattg actattagat tctatttaaa aagacttaga atgcacatga
    12061 aagtatgtat tctaggtctt ttgttttgta tacatttaat tttcaagagt attatctatt
    12121 tttccatgca tggtaacgag tatcaatcaa taactgggta aggcgaccca aggtttctct
    12181 ttcttctgga gtgagggatt gagcttggac aaagagatga tgaatgtatt caagcgcctt
    12241 taaggaagcc aagtcattga tagagctaat accagcatca agaatagtgc caaagaagag
    12301 gtcgaagtag agctgaagtt cttcgtcagg gactgtggcc acccattctt taaaggttgt
    12361 gtctacttgc cggctatcac tgttggtctt atccagttga acgaagtgct tgtcctcaat
    12421 ctgccaacta aaggtatcgt gctgggcgat gccacccagg gcagtactct gaacgatgat
    12481 ttggtgagca ggaatttcca gcatcatacc gataatagac ccttgtggaa tgaatatctt
    12541 gcttctatcc attatccttt gataacccgc agtctgtgtc aattcttgat ggagaccagg
    12601 tgcatcaaat gtataaactg ctgtgatctg attttgcaaa ctttgctcaa tttggctagc
    12661 agcatagata gcgagatttc ctcccttgga atgcccagcc agaataacct tttgcttagg
    12721 atgatgggca aaaaagttct ttaaatagcg aagggcgtgc ttttgagcag gaatttcctt
    12781 catataggtc aggtggaaat cttccttcca gccaatgata ctgtcatctg tcccacgaaa
    12841 gacaatcaga taggtatcga ggctgacacg ataagtcata gccgcaaatt gcttttgcag
    12901 ttcagggtcg atgtcgttga taaaatggga gagtttgcaa tttttgaagc gcttgtgttg
    12961 agccaattca tctaataatt gaaggcgatt tttgctagta agcatggtgg gctctcttgg
    13021 aacctgaggt gctagatcta aaagacgttg aggaagtgtg gagaccagat tatcaaagga
    13081 gaggtaggtg atttctatta aggttagaat gtctaactca ttcaagggaa ggtcgtaata
    13141 agaatcatat gcgacatctt tcagatagtc aaaaatattg gccataagag ctcctttctt
    13201 tttatttatc ttagcaaatt tccctcaaat tagctagtag catagcctgt ttgtactggc
    13261 taaaaacagg ctatttcaaa ttcagtttca gaccatctag catggaaaaa tctgttataa
    13321 taatggaaaa ggagaagcgc atgcacaaga ttttattaat agaagatgat caggtcattc
    13381 gtcaacagat tgggaaaatg ctctctgaat ggggatttga agtggtcctg gtagaagact
    13441 ttatggaagt tttgagtcta tttgttcagt cggaacctca tctggtcctc atggatattg
    13501 gtttgccctt gtttaatggt tatcactggt gtcaggaaat ccgcaagatt tccaaggtac
    13561 ctatcatgtt tctttcttcg agagaccagg ctatggatat tgtcatggca atcaatatgg
    13621 gggcggatga ctttgtgacc aagccttttg accagcaggt tcttttagct aaggttcagg
    13681 gcttgttgcg tcgttcctat gagtttgggc gtgatgagag tttgctggaa tatgctggtg
    13741 ttatcctcaa taccaaatcc atggatttac attatcaagg gcaagtcttg aatttgacca
    13801 agaatgaatt ccagatttta cgcgtgttat ttgagcatgc aggcaacatc gtagcacgtg
    13861 acgacctgat gcgggaactt tggaacagtg actttttcat tgatgataat accctctctg
    13921 tcaatgtggc tcgtttgcgt aaaaagttgg aggagcaggg attggtagga tttatcgaga
    13981 ccaagaaagg aatagggtac ggattgaagc atgcttgatt ggaaacaatt ttttctagcc
    14041 tatctgcgct cccgtagtcg tctttttatc tatctgcttt ttttggcatt tcttgtctta
    14101 ctctttcagt ttttatttgc cagtctagga atttacttcc tctacttttt cttcttgtgt
    14161 tgctttgtaa ccatcttatt tttcacttgg gacatattgg tggaaacgca ggtctatcgc
    14221 caggaacttc tctatggaga gagggaagcc aagtctcctt tggaaatagc tttagcagaa
    14281 aaattagaag cgcgtgagat ggaactctat cagcagaggt caaaagcaga aagaaaactg
    14341 acggatttgc tggattacta taccttgtgg gttcatcaga taaagacccc cattgcagcc
    14401 agtcaactct tagttgcaga agtggtcgac cgccaactga agcagcagct agaacaggaa
    14461 attttcaaaa tcgactccta taccaaccta gttttacagt acctgcgttt agaaagtttc
    14521 catgatgatt tggtcttaaa gcaggttcaa attgaggact tggtcaagga aataattcgt
    14581 aaatatgctc ttttctttat tcaaaaaggc ttaaatgtca atctacatga ccttgataaa
    14641 gaaatcgtga cggataaaaa gtggctgcta gtggttattg agcaaatcat ctcaaacagt
    14701 ctcaagtaca ccaaggaagg tggtctggag atttatatgg atgaccaaga gctttgtatc
    14761 aaagatacgg gaatcgggat aaaaaacagt gatgtcctcc gagtatttga acgtggcttt
    14821 tcaggataca atggccgttt gacccagcag tcctctggac ttggccttta tctatctaag
    14881 aaaatttctg aagaactggg gcaccagatt cgtatcgagt ctgaggtcgg aaaaggaacg
    14941 acagtgcgga ttcagtttgc tcaagtgaac ttagtccttg agtaaaaata gatgagaaaa
    15001 agcctccaga ttggaggctt ttatgctaga ctcttatttc tcaacgcgtg atttgttggc
    15061 gatatcagct aggatagctt gaacaaaatt atcaactgag acagtttgtg tttctttttg
    15121 gccgtagcga cgaacgttga ctgtttcgtc ttccatttct ttgtctccaa caattaattg
    15181 gtaaggaatc ttgctggttt gtgaagcacg gatcttgaac tgcatttttt cattgcgctc
    15241 atctacgtct gcacggacac cgcggtcacg gagtttcttg gccacttccc aagcgtagtt
    15301 cacgtgtttt tcgttagata ctgggatgag ggttacttgg tgtggtgcca gccatgttgg
    15361 gaaggccccc ttgtagttct caatcaagat agctgtgaag cgttccatag ttgagataac
    15421 cccacggtgg atcatgactg gacggtgatc ttcgccatca gctccgatgt atttgaggtc
    15481 gaagcgttct ggcaacaaga aatcaagttg gatagtagaa agggtttctt cttttccaag
    15541 ggcagtttta atctggatat ccaattttgg tccgtagaag gctgcttcac cttcggcttc
    15601 aaagtagtcc acgcccattt catcaagagc tgcacgaagc atggtttggg cattttccca
    15661 catctcatcg ttatcaaagt acttatgagt atcttgaggg tcacgaagag agaggcggaa
    15721 gcggtagtca gtcaagttga agtcttcata aacatcgata atcaactgaa gggcacgttg
    15781 gaattcttct tggatttgtt ctggagtaac gaataggtga ccgtcgttga gtgacatttc
    15841 acgtacacgt tgaaggccag tgagggcacc agatttttcg taacggtgca tcataccgat
    15901 ttcagcgata cggattggca attcacggta agagtgaaca tggtgtttga aaacttggat
    15961 gtggtgcgga cagttcattg gacgaaggac aaattcttcc ccgtcaccca tgtccatggt
    16021 tgggaacatg tcttcttggt aatgatccca gtgaccagaa gtcttgtaaa gctcaacaga
    16081 agcaagtggt ggagtgtaga cgtgttggta gccagaagcc aactctttgt ttacgatgta
    16141 gcgttccaat tcacgacgga tagtcgcacc atttggcaac cagaatggca aaccttgtcc
    16201 cacttcttgt gaaatcataa agaggtcaag ctctttacca agtttacggt ggtcacgttc
    16261 cttagcttct tcacgcattt gaaggtagtt tttcaagtct ttcttgtcaa accaagctgt
    16321 accgtagata cgttgcatca tagcgttgtc gctgtttcca cgccagtaag caccagctac
    16381 atggagaagg tggaagattt ggatacgacc tgttgatgga acgtgaggtc cacggcagag
    16441 gtctacatat tcaccctgac gatagatagt caaaccgcct tcgtcttctg agtgttcttc
    16501 aatcaattcc aacttgtaag ggtcattttt gaagatttca cgtgcctcgt ctttagtcac
    16561 ttcttcacga atagatggga agttttcttt gacgattttt tgcatttctt cttcgatacg
    16621 aggaaggtct tcgttagaga tttgaccagc tatgttgtca gtatcgtagt agaaaccatc
    16681 ttcgatggct ggaccaactc ccaagtgaat gtctgggaaa agacgacgag ctgcttgggc
    16741 gaacaagtga gctgctgagt gacgcaagat tggaagggca tcttcgtgat caggtgtcac
    16801 aatttcgatg cttccatctt cagtgatagc gcgagtagtg tcgatgagtt tgccgttgaa
    16861 tttaccagcc aaggcttttt tagctaggga attgctgata gattgggcaa tttcaaaagt
    16921 tgttacgcca gattcgaatt cacgaacagc gccatctggg aaagtaatgt taatcatgat
    16981 gttctccttt tttattattt catagtttct aagcattgat tttttatcag cagactactt
    17041 ccaagtccta acgttcaatt gcttttcttt atcagagagg aagttttcca tttccgtcag
    17101 aatgtctata tccaaacgtt gagaaagttc taatagccac cagatatttt ctgaaagttt
    17161 ttgttccagt gtgtagggtg tttcatcata gtagcgtcct tgctttgtca tcaccaatcg
    17221 ttggaaattt ccaatatcat tagataaagc caagaggtct tcttctaccg tccacttgga
    17281 atcatgatgc ttaacttcca gttcgtgata agcttgtcgg attgcccaac tgtgctccac
    17341 taattcttgc aactccataa ttagtatcct aataaaaaaa tgcgacatcc tcgaaaggac
    17401 gtcgcaacgt ggttccacct tcatttatgt acctcaaaag agagggacac ctctgatcgg
    17461 ctctaacgtg gccaccgttt tatgttttca taaaaaactc aagagtagta tcagtttaga
    17521 cttcctatgc gtttccagca accacgcact ctctagtaga aagggactaa gtgacttgtc
    17581 tctatggatt attatagcat gattgtgaga tttttcaagg attttgtgaa agttttttgt
    17641 ttttcatttt ttgtagaata tgcttgatgc taataccaga atccatgccc accagagcaa
    17701 ctgtttcata gattagcaag taaattagta taaactcacc aaagattatg atagtgaagt
    17761 aaaagagcag tccgttacca actaaccatc aaagtgagaa acggaaacgg tagctataga
    17821 gtaacgatac aatcaagatt accagaggtg taaagaagag aagattgttt atatagagtc
    17881 ccttgagtgt aagggggtca gaaacaacta gcaagaacaa ctcataaagt ggccaataaa
    17941 tatgaccaga tagtagggga gatgggaata gaatctacgc atgataatca cctcgtttta
    18001 aaaaaaacag acaacttaga tctcttgcta tgattattat aagcatgttt aaaacaagaa
    18061 agtaaatttg ttgacaaaga agatttttct tttcactaga atcatagtta gttttcttgg
    18121 tagaaatatt ttgataataa ttaagcaaga ctattgacat tgattattaa gagtgtcaca
    18181 attgctgtaa ctaaaataat tacaacaaga gaggtataaa aaatgaaact agcagttatt
    18241 gcagcaaatg gacaagtggg gaaagcaatt gttgaagaag cagtcaaacg aggacatgaa
    18301 gtaacggcta ttgttcgttc tgaaaacaaa agtcaagctg agtctattat taaaaaagat
    18361 ttatttgagt tgacaaaaga tgatcttact ggatttgatg ctgttatttc agcgtttggt
    18421 gcttatacac cagatacgct accgctccat agcaaatcaa tcgaactatt taatcagctt
    18481 cttgctggaa cacagacacg ttttctagtt gtagggggag cagaaagtct ttatatagat
    18541 gaaacaaaaa cgacacgact tttagatacg tctgattttc ctgaagaatt taagtcacta
    18601 gcaaaagctc aagcggatga attggatctt cttcgtacaa aaaataatct caactggact
    18661 tttgtcagtc ctgctgtaga ttttattccc gatggtgaaa aaacaggaaa ctacattcta
    18721 gcaggagaaa tttttactac taatgaaaaa aggaataagc cagattagtt atgcagatta
    18781 tgctattgga ttggtagatg aacttgaaaa aggtcatcat ataaaagaac gtatctcttt
    18841 acttgaaaaa taataactat gagagtatta atgtttgaca gaaaatttaa atcttggtaa
    18901 aatagggatt gtcgtaaaga caaataactt cttcttggtt acaggcatgc caacctgtca
    18961 ctcggatgaa gccaaataaa aaggagaaac atcatggcaa tctcaaaaga gaaaaaaaat
    19021 gaaatcattg cacaatatgc acgtcacgaa ggtgatacag gttcagtaga ggttcaagtt
    19081 gctgtcctta cttgggaaat caaccacctt aacgaacaca tcaaacaaca caaaaaagac
    19141 cacgctactt accgtggatt gatgaaaaaa atcggtcgtc gtcgtaactt gcttgcatac
    19201 ttgcgtaaaa acgacgttaa ccgttaccgt gagttgatca actctctagg acttcgtcgc
    19261 taattcaaga tacaaaggcc gtcaaaagca caaagcaaaa ataggaaaat tgacgaagaa
    19321 acttcagttt ctaggagatt ttatcttttt tgccaagtgc ttaggccgtg ttcaattgag
    19381 catatcttga taataaagct actctaaatt gggtagcttt tttgcgttgt caccttacct
    19441 cgctatactc aagtataatc tttggttacg gttcctagca ctgtaaggta aaataaacca
    19501 gcttttctcc cttcggggag attttttttg tttcactgaa agtttcgtac gatcttcgcc
    19561 tcgtcgccta gtctataaac gatttatcta gcaagaatca tgatgctaag ggcgtcaaaa
    19621 atccgtatga aaatagggaa atgacacagt gttcgatgag cacaaggagt ttcatctttt
    19681 tcactaggat tttagcccgg gctaaaatca gctctctgac ttcagagggc ttttttattg
    19741 aggttttatc ggtaacaatt ttggagacta caaaaacctc aaatggtatc agctaattac
    19801 accataaagg tgcgcagtta ctcggctttt caagccgagt agctgtatgc aagcccccta
    19861 gaagagccca ctctttacaa agtaaagtat agtatgttat actttacatg gaagtagtca
    19921 ctgaattcca gttagaaatt actttgtaac tacgttttga ggaggagtaa aatgctttcc
    19981 tacgttcgac attacccact agcgatagct aaattaatgt gtctgtgctc tcctgaaatc
    20041 tgctgattta ttactgacta atacaggagg ttttttttat gggacagaca atcatatctg
    20101 ctattggtgt ttatatttcc accagtatcg attatttaat tattttaatt attttatttg
    20161 cacagctatc acagaataaa cagaaatggc atatttatgc ggggcaatat ctaggcacag
    20221 gcttacttgt aggggcgagt ttagttgctg cttatgtcgt taatttcgtg cctgaagaat
    20281 ggatggttgg attgcttggt ttaatcccta tctatttagg gattcgcttt gcaattgttg
    20341 gagaagatgc ggaagaagaa gaggaagaaa ttattgaaag attagaacaa agcaaggcaa
    20401 atcaactgtt ttggacagtt acattgctga caattgcgtc tggcggagat aatttaggta
    20461 tctatatacc ttattttgct tcgttagatt ggtcacagac cctcgtggcc ttgcttgtgt
    20521 ttgtaatcgg cataattatc ttttgcgaga ttagtcgggt gttatcctct attccgttaa
    20581 tattcgagac aattgaaaaa tacgagcgaa tcattgtgcc cttagtattc attctacttg
    20641 gactatacat catgtatgaa aatggcacga tagagacttt tctgatcgtg tagatttttt
    20701 tgtttcacta ggattttagc ccgagctcaa atcagctctc tgattttcag agggcttttt
    20761 cattgagtat aaatggacaa tcttaaagtt actctattgt agagtggctt ttgatttgat
    20821 taggagattc ttataaatat agtaaaatga aacaaaaaca ggacaaatcg atcaggacag
    20881 tcaaatcgat ttctaaaaat gttttaagaa gtagaggtgt actattctag tttcaatttg
    20941 ctatataaat gaaaacctta gctgtttggc caaggttcat ttcttctatg caagttcttc
    21001 tcttttctta tctgggttcc agatgaagct tgcgataaag ctaaagatga tggttaggat
    21061 agcgaggata atagcaagga tgagggcagg gatgcggata aaccaccaga gtggatttgg
    21121 ttttttatca ttgtgccatt gtttcgtttc ttcgtccaga cgttggaatt ctgtttgaat
    21181 acgattggcg actgtttcaa tgccttcatc attcattttc ttgatatctg agatatcgat
    21241 tggatttcca aagttcatat cgacacgttc acggctaatc aagcccttca aaatcatggg
    21301 accggtgtag gtaaccggca tgatacggac cttggccatt ttggcaatca gtgctgcgcc
    21361 ccccttgaca tcgtttgagt ggcggctacc acttggaaac atgatgagag agcggtcact
    21421 ttttttgaga acgttgatag gatatttgat ggctgaggcg ctgggatttt cacggtcgat
    21481 gggaaaggcg ccacacatac gaatccacca accaaagata cggttggtaa agagttcttt
    21541 ttttgccata aagatgaact gttttggctt ggtcgcaaag gccatataaa caggatccca
    21601 ccaggtacgg tgaggcgcaa ctaaaatata attttcatct tgattaggaa ttttatcagt
    21661 attatgatag tgagcattgc cattgatgga ccataagagc aacacaacta atccacgcaa
    21721 ataagtataa aacatgcgat ctccttcgat tgttttcttg ttattattat accttatcaa
    21781 aggagggctg gcaaactttt cccttgacta gatgcatatt tgggatgaaa ttagaattct
    21841 gttaaaaaaa atggtataat agaatttatg gataaaaata agattatggg attaacccaa
    21901 agagaagtca aggaaagaca ggctgagggt ttggtcaatg actttaccgc atcagccagt
    21961 accagcactt ggcaaatcgt taaacgaaat gtctttaccc tttttaacgc tttgaacttt
    22021 gccattgctt tggcccttgc ctttgtgcag gcttggagca atctggtctt ctttgctgtt
    22081 atctgcttta acgctttttc tgggattgtg accgagctac gagccaaaca catggtggac
    22141 aagctcaatc tcatgaccaa ggaaaaggtc aaaaccatcc gtgatggtca ggaagttgct
    22201 cttaatcctg aagaattagt gctaggagat gtcattcgtt tgtctgcagg agagcagatt
    22261 cctagtgatg ccttggtttt ggaaggcttt gcggaagtca atgaagccat gttaacggga
    22321 gaaagtgatt tggtgcaaaa ggaagttgac ggcttacttt tgtcaggaag tttcctagcc
    22381 agtgggtcag ttttatctca agttcaccat gtcggtgcag acaactatgc tgccaaactc
    22441 atgcttgagg ctaagaccgt taaacccatc aactcccgta tcatgaaatc gctggacaag
    22501 ttggctggtt ttactgggaa gattatcatt ccctttggtc tggctctctt gctggaagcc
    22561 ttgcttttaa aaggcctgcc tctcaagtca tccgttgtaa actcgtcgac agctcttttg
    22621 ggaatgttgc ctaagggaat tgcccttttg accattactt cgctcttgac tgcagtgatt
    22681 aagttgggct tgaaaaaggt cttggtgcag gagatgtact ctgttgagac cttggcgcgc
    22741 gtggatatgc tctgtctgga caagacgggc accatcaccc aaggaaagat gcaggtggag
    22801 gctgttcttc cactgacgga aacttacggt gaagaggcta ttgccagcat cttgactagc
    22861 tacatggccc atagtgagga taagaatcca actgcccaag ccattcgcca gcgttttgtg
    22921 ggagatgttg cttatcctat gatttccaat cttcccttct cgagcgaccg caagtggggg
    22981 gctatggagt tagaaggctt ggggacagtt ttcttagggg cacctgagat gttgctggat
    23041 tctgaagtcc cagaagccag ggaggccttg gagagaggat cacgtgtctt ggtcttagct
    23101 ctcagtcagg agaaattaga ccatcacaaa ccacagaaac catctgatat tcaggctcta
    23161 gccttgctgg aaatcttgga ccccattcga gagggagcag cagagacgct ggactatctc
    23221 cgttctcagg aggtgggact caagattatc tctggtgaca atccagttac ggtgtccagc
    23281 attgcccaga aggctggttt tgcggactat cacagctatg tagattgctc aaaaatcacc
    23341 gatgaggaat tgatggccat ggcggaggag acagctattt tcggacgtgt ttcccctcat
    23401 caaaagaaac tcatcatcca aacgttgaaa aaagcgggtc atacaacggc tatgacaggg
    23461 gacggggtta atgatatctt ggcccttcgt gaggcggatt gttctatcgt gatggcggag
    23521 ggggatccag caacccgtca gattgccaat ctggttctct tgaactcaga ctttaatgat
    23581 gttcctgaga ttctcttcga gggtcgtcgc gtggtcaata acattgccca catcgccccg
    23641 attttcttga taaagaccat ctattccttc ctgttagcag tcatctgtat tgccagtgct
    23701 ttactaggtc ggtcagagtg gattttgatt ttccccttca ttccgatcca gattaccatg
    23761 attgaccagt ttgtggaagg tttcccacca ttcgttctga cttttgagcg aaatatcaaa
    23821 cctgttgagc caaatttcct cagaaaatcc atgcttcgtg ccctaccaag cgctctcatg
    23881 gtcgtcttca gcgtcctgtt tgtgaaaata tttgggagta gccaaggttg gtctgagtta
    23941 gaaatctcaa ctctactcta ttatctcttg gggtcaattg gtttcttatc cgtatttaga
    24001 gcctgcatgc catttaccct atggcgtgtc ctcttgattg tttggtcagt aggaggtttc
    24061 ctagccacag ctctcttccc aagaattcaa aaactgcttg aaatttcaac cttaacagga
    24121 caaacattac ctgtttatgg tgtcatgatg ttggtcttta ccgtgatttt catcctgacc
    24181 agtcgttacc aagcgaaaaa ataaagaaag actgcaatct gtggattgcg gtttttttag
    24241 gtacaagatt gccagccgaa atgtggtata ataagaggta atagagtttg gaaagtgaga
    24301 gaaaatgatt tcaaagagat tagaattggt agcttccttt gtgtcacagg gggctatttt
    24361 actagatgtg ggaagtgacc atgcttatct gcctatcgag ttggttgaga gaggccaaat
    24421 caaaagcgct attgcaggtg aggtggtgga aggtccctat cagtctgcgg ttaaaaatgt
    24481 tgaggctcac ggcctaaagg agaaaatcca agtccgttta gccaatggct tggcagcttt
    24541 tgaagagact gaccaagtgt ctgtcattac cattgctggc atgggtggtc gtttgattgc
    24601 taggatttta gaagaaggtt tggggaagtt agctaatgta gagcgtttga tcctccagcc
    24661 caataatcgt gaagacgact tgcgtatctg gctacaggat catggattcc agattgtagc
    24721 agaaagcatc ttagaagaag ctggaaagtt ttatgagatt ttggtggtgg aagcaggaca
    24781 aatgaagcta tcagccagtg atgttcgctt tggtcccttc ttgtccaaag aagtcagtcc
    24841 agtatttgtc caaaaatggc aaaaagaagc tgagaagcta gagttcgccc tcggacaaat
    24901 cccagaaaaa aatctggaag aacgtcaagt tctagtagat aagattcaag ctatcaagga
    24961 ggtgccccat gttagcaagt gaagtgatcc aagcttatga agccttttgc cctcaggaat
    25021 tttctatgga gggagacagc cgtggtctgc aaattggcac tttagacaag ggtatccaaa
    25081 gggtcatggt tgccctcgat attcgtgaag agacagtggc agaggccatt gaaaagggtg
    25141 tggacttgat tatcgtcaag cacgcgccta tctttcgtcc tatcaaggac ttgcttgcca
    25201 gccgtccgca aaatcagatt tacatcgacc tgattaagca tgatatcgca gtttatgtca
    25261 gccataccaa tattgatatc gttgaaaatg ggctcaatga ctggttttgt cagatgctag
    25321 gaatcgagga gacgacttat ctgcaggaaa caggtccaga acgtggaatt ggacgtattg
    25381 ggaatattca gcctcagaca ttttgggaat tggcccaaca ggtcaagcaa gtctttgacc
    25441 tagatagcct tcgaatggtg cattatcaag agaatgattt gcagaagcct atttcaagag
    25501 tagcaatttg tggtggaagc gggcagtctt tctataagga tgctttggca aagggggcag
    25561 atgtctatat cactggtgat atctactacc atactgctca ggatatgctg tctgatggct
    25621 tgttagcatt ggatccaggt cactatatcg aagtgctttt tgtggaaaaa atcgcagcac
    25681 tccttactca atggaaagag aaaaaaggct gggaactaga gattttacct agtcaagcat
    25741 cgaccaatcc tttccgtcat atctagttag aaagtgaaga caatgaaaaa agttgccatt
    25801 attggagcag gaattgtggg agcaacagct gcctactacc tctcgagaga aagtgaccta
    25861 gaggtgaccg tttttgacca tgagcaaggt caagccacca aggccgcagc aggaattatc
    25921 agtccttggc tttcaaaacg ccgcaataag gcctggtaca agatggcgcg attgggggct
    25981 gacttttatg tggatttatt agctgattta gagaaatcag gacaagaaat cgacttttac
    26041 cagcgttcgg gagtctttct cttgaaaaag gatgaatcca agttggaaga actctatcaa
    26101 ctggctctcc agcgcagaga agaatctccc ttgataggcc agttatccat tctggaccaa
    26161 gccccagcta atgaattatt ccctggcttg cagggattcg acaacttgct ctatgcttct
    26221 ggtggagcga gagtagatgg tcacctctta gtaactcgtt tgctggaagc cagtcaggtc
    26281 aagttggtca aagaaaaagt gactctgaca ctcttatcat caggctacca gattggcgaa
    26341 gaggtgtttg atcaggttat tttggcaacg ggagcttggt tgggggacat gttagagcct
    26401 ttaggttatg aagtggatgt ccgtcctcaa aaaggacaac tacgagatta tcagcttgcc
    26461 caagacatgg aagattaccc tgttgtcatg ccagaagggg agtgggattt gattcccttt
    26521 gcaggtggga aattatcctt gggcgctacc catgaaaatg acatgggatt tgatttgacg
    26581 gtagatgaac ccttgctcca acaaatggag gaggcggcct tgcctcatta cccagtttta
    26641 gctagagtga cttcaagggc tgagcgtgtg ggaatccgtg cctataccag tgatttctct
    26701 cccttctttg ggcaggtgcc agaattggca ggtgtctatg cagccagtgg actaggttca
    26761 tcaggcctca caactggtcc tatcattggt taccatctag cccaactgat ccaagacaag
    26821 gagttgacct tggaccctga aaactaccca attgaaaact atgtcaaacg agtaaaaagc
    26881 gaataatact caatgaaaat caaagaacaa actaggaaac tagccgcgga ttactcaaag
    26941 cactgctttg aggttgtgga tagaactgac gaagtcagct caaaacactg ttttgaggtt
    27001 gtggatagaa ctgacgaagt cagtaaccat acccatggca aggcgacgtt gacgtggttt
    27061 gaattggatt ttcgaagagt ataagcattt tactgaaatt ttatcaggta gtttaggatg
    27121 gcaaatgaca ttccctgtca aaaatggtaa aataagaaaa aataatctga gaatcgagga
    27181 aaaaagatgc aagaaaagat tttggtaact ggtggtgccg gttttattgg aacccacact
    27241 gttattgaat tgatccaagc aggccatcaa gttgttgtgg tggataatct tgtcaatagc
    27301 aatcgtaaga gtttagaagt tgttgaaaga atcacaggag ttgagattcc tttctatgag
    27361 gcagatattc gtgacacaga taccctcaga gatattttca agcaggaaga accgactggt
    27421 atcattcact ttgctggttt gaaggctgtt ggcgaatcaa cacgtatccc tcttgcctac
    27481 tatgacaaca atatcgctgg aactgtcagc cttttgaaag ccatggaaga aaacaactgt
    27541 aaaaacatca tcttcagttc ttctgcgaca gtttacgggg atccgcacac agtgcccatc
    27601 ttggaagatt tcccactttc agtgaccaac ccatacggtc gtactaagct catgctagag
    27661 gaaattttga ctgatattta caaagcagac tcagaatgga atgttgtctt gcttcgttac
    27721 tttaacccaa tcggagccca tgagagtggt gatttgggag aaaatccaaa cggtattcca
    27781 aacaatctct tgccatatgt gactcaagta gccgttggaa aattagagca agtgcaagtg
    27841 tttggagacg attacgatac ggaagatgga acaggtgttc gtgattatat ccacgttgtc
    27901 gatttggcta agggtcacgt tgcagctttg aaaaaaatcc aaaaaggttc aggactaaac
    27961 gtttataacc ttggaactgg taaaggttac tcagttcttg aaattatcca aaacatggaa
    28021 aaagcggtgg gacgtcctat tccttaccgc atcgtagaac gtcgcccagg tgatatcgct
    28081 gcctgctact cagacccagc aaaagctaaa gcagaactcg gttgggaagc agaactcgac
    28141 atcacccaaa tgtgtgaaga cgcatggcgt tggcaaagca agcatccaaa tggatttgaa
    28201 gactaagatg atgatttcaa tcatcgtccc ttgtttaaac gaagaggaag tacttcctct
    28261 tttttatcag gctctggaag ctttacttcc agatttggaa acagaaatcg agtatgtctt
    28321 tgtcgatgat ggatcaagtg atgggacctt ggaactctta aaggcctatc gggagcaaaa
    28381 tccggcagtc cattatattt ctttctctcg aaattttggc aaagaagcag ccctttatgc
    28441 aggcttgcaa tatgcgacag gagatttggt ggtggtgatg gatgcagacc tccaagatcc
    28501 tcctagtatg ttgtttgaga tgaaaaatgt actagacaaa aatgtagact tggactgcgt
    28561 tgggacacgg agaactagtc gggagggaga acccttcttt cgcagtttct gtgctgttct
    28621 cttttatcgc ctcatgcaaa aaatcagccc agtagctctg ccgtcgggtg tccgtgattt
    28681 tcgtatgatg agaaggtctg tagtcgatgc cattttaagc ttgaccgagt ccaatcgttt
    28741 ttcaaagggg ctcttcgcct gggtgggttt caaaacctac tatttggact atccaaatgt
    28801 cgaaaggcag gctggcaaga caagttggag ttttaggcaa ctctttttct actccattga
    28861 agggattatt aatttttcag attttccctt gattatagcc tttgtagctg gtctcctatc
    28921 ttgttttctt tctctgctga tgaccttttt tgttgtggtt cggaccctca ttttgggcaa
    28981 tccgacatct ggttggacct ctctgatggc tgttattctc tttcttggag gcattcaact
    29041 cttgaccatt gggattctcg gcaagtatat cagtaagatt tatttagaga ctaaaaaaag
    29101 accactttat cttatcaaag aaaaaagtga ccttcctgat tttacagaaa aaaataaagt
    29161 gaaaagacta taattttaca tggaaatgtg ctaaactaga gggagtggat tatcgccatt
    29221 gatttaggct tcctggtttc caatcagggc gcgcgttggg caatttttaa cagcctctaa
    29281 gacatcctga ctaggagaaa tttctttttc cagttggtca gggtcatcgt aaaaacgcac
    29341 gattccatta tcgtggtaat caaataaatc agaataagtt tggcaaagcc cacaggcgat
    29401 acatcgttca ggtataagtg tgattttcat gttcatattt atattgtaat aagaaaagta
    29461 aaaaaaaaca aggagtaagg tatggcaaaa gaaccgtggc aagaagatat ctatgatcaa
    29521 gaagaatcaa gagcagagcg tcggcatcga aaccacggag gggctgatag gatggctaat
    29581 cgtattttga cgatcctagc tagtattttc tttgtaattg tggtggtgat ggtcatcgtt
    29641 ctcatctatc tatcatcggg gggaagtaat cgcacagcag ccttaaaaga ctttcatgat
    29701 tctgatgcaa gtgtagtaca aatctcatct tcaagcagtt ctcagcctga gcagagttca
    29761 gagccagaat ctacttctag tagttcagaa gaagctgcta atcctgaagg aacgattaaa
    29821 gttctcgcag gagaagggga agcagctatt gccgctcgtg caggaatctc cattgctcag
    29881 ttagaggcct tgaatcctgg gcacatggct acaggatctt ggtttgctaa tccaggtgat
    29941 gttataaaaa taaaatagga gccgatatga aaacaattca aattgctatt gatggtcctg
    30001 cttccagcgg taagagtacg gtcgcaaaga ttattgctaa ggattttgga ttcacctacc
    30061 ttgatacagg agctatgtat cgtgcagcga cctatatggc tcttaagaac caattaggag
    30121 ttgaagaagt cgaagccctt ctagccttgt tggaccagca tccaatcagc tttggacgtt
    30181 cagaaactgg agaccagctt gtttttgtag gagatgtgga tattacccat cctatccgtg
    30241 aaaatgaagt gaccaatcat gtttctgcta ttgcagcaat tcctgaagtg cgtgagaaac
    30301 tggtttctct ccaacaagaa attgcccagc aaggcgggat tgtcatggat ggtcgcgata
    30361 ttggaactgt tgtattgcca caagcagaat tgaaaatttt cctagtagct tctgttgatg
    30421 agagagcaga gcgtcgttac aaggaaaata ttgccaaggg aattgaaaca gaccttgaaa
    30481 ccctaaaaaa ggaaattgct gcgcgtgact acaaggatag tcatcgtgag acttctcctc
    30541 tcaaacaagc agaggatgct gtctaccttg atacaactgg tttgaacatt caagaagtag
    30601 ttgaaaaaat caaagcagaa gctgaaaaaa gaatgtaaat gtagaaaagc cgatagattg
    30661 atatcggttt tttctaaatt tgtcaaaatg ctgattttag ggtataatag ttgctgttcg
    30721 agaaattttg attttcaaag aatagtgaat gatgataagg agaaatcatg aacaatttac
    30781 caaattgtcc aaaatgtaac tcagagtatg tctacgaaga cggtgcccta ctggtttgcc
    30841 cagagtgtgc tcatgagtgg aatcctgctg aagttgcaga agtagaagaa ggtcttgtcg
    30901 ctatcgatgc caacggaaat aaattggctg atggtgatac agtaactctc atcaaggact
    30961 tgaaagtaaa aggtgcgcca aaagatttga aacaagggac gcgcgtgaaa aatatccaca
    31021 tcgtagaagg cgaccacaat atcgactgta agattgatgg ttttggtgcc atgaaactta
    31081 aatcagagtt tgtgaggaag atttaagcat gtcaaaacac tataaacttg tattttatag
    31141 ccgtatcttc ttgtttctag cggcttttac gggagtttat cttgaaatta ctaagcatgg
    31201 tggttttggg atgcttctct attacacggt tctgtccaac ctcttggtaa ctatttttac
    31261 cctttatctt ctaaaggtta tgagccgtgt aggtgaaaat tggcaaagac caagtctctt
    31321 gcgcttaaaa ggtggggtca ccatgagtat catgattacc tgtgtgattt accatttcct
    31381 cttggcgccc attgcgacta atttctatac cctagaaaat ttcctttgcc actatatcgt
    31441 tcccatctgg tttttagcgg ataccctctt ttttgacaaa cagggtcaat acaagatttg
    31501 ggatccagca gtgtggacca ttttaccctt tctgtatatg atgtttgctc tttttaatgg
    31561 cttggttcta aaactcaata ttccaaatgc caaggataat cctttccctt acttcttttt
    31621 gaatgtgaac aagggttgga atgttgtgtt taagtggtgt ctgattatct ttgttgccta
    31681 tatggtagca ggatttattt tctactttat caagcaaatc aagagaaagt catcctaata
    31741 ctcttcgaaa atctcttcaa accacgtcag tttccatctg caacctcaaa acactgtttt
    31801 gaacaacctg cggctagctt cctagtttgc tctttgattt tcattgagta taagatacaa
    31861 gggattcatt ttcgaattta agaaggagtc tacaaaccaa tgaggcttct tcttttcttg
    31921 cttatttgat aactatcaaa aaagtagaag attaagagaa atatagaaag agatttcatg
    31981 aaacaatttt tagaacgggc cagcattttg gctctctccc tcgttttgat tacctccttt
    32041 tccatttcga gtgccctacc agccatgttt gactattatc agggttattc taaggaacaa
    32101 attgagctct tggtgagctt gccttccttt ggaatcatga tgatgttact gctaaatggt
    32161 ttcttagaaa aaatatttcc tgagcgctta cagattagtt tgggcttgct gattttatca
    32221 ttgagcggta cagctccctt ctggtaccaa gcctatccct ttgtctttgg agcacggctt
    32281 ctctttggtt tgggtcttgg gatgatcaat gccaaggcca tttctattat cagtgaacgc
    32341 taccaaggaa aaaggcgaat tcagatgtta gggctacgcg cttctgcaga ggtcgttgga
    32401 gcttctctca ttaccttggc cgtcggtcag ttgttggcct ttggttggac agctatcttt
    32461 ctagcctata gtgctggatt tttggtgctg cccctttatc tgctctttgt cccttatgga
    32521 aaatcaaaga aagaagtcaa gaaaagagcg aaggaagcaa gtcgtttaac tcgagaaatg
    32581 aaaggcttga tttttacctt agctatcgaa gcggcagttg tagtttgtac caatacagct
    32641 attaccatcc gtattccaag tttgatggtg gaaagaggat tgggggatgc ccagttatct
    32701 agttttgttc ttagtatcat gcagttgatc gggattgtgg ctggggtgag tttttctttc
    32761 ttgatttcta tctttaaaga gaaactgctc ctctggtctg gtattacctt tggcttgggg
    32821 caaattgtga ttgccttgtc ttcatccttg tgggtggtag tagcaggaag tgttctggct
    32881 ggatttgcct atagtgtagt cttgacgacg gtctttcaac ttgtctctga acgaattcca
    32941 gctaaactcc tcaatcaagc aacttcattt gctgtattag gctgtagttt cggagccttt
    33001 acgaccccat ttgttctagg tgcaattggc ttactaactc acaatgggat gttggtcttt
    33061 agtatcttag gaggttggtt gattgtaatc tctatctttg tcatgtacct acttcagaag
    33121 agagcttagg attgattcct aagtttttct tttgatactc aatgaaaatc aaagagcaaa
    33181 ctatagttga ttgagtttgg aatagtacgc tgtagctgct aaaatatttc tagaaattaa
    33241 tttgactttc ctaatagagt tattcatatc ttatttcaat ctactataga aagctagccg
    33301 caggttgctc aaaatagtgt tttgaggttg cagatggaaa ctgacgtggt ttgaagagat
    33361 tttcgaagag tatgagattt ttatacccat acaatatttg ttttaggtcc ttgtttacag
    33421 actaatttct tgataaaata gaggttatga cgagatataa agcaactatt tcctatgatg
    33481 gttatgcctt tgctggcttt cagcgccagt ctcatgcgcg tagcgttcag gaagaaattg
    33541 aaaaaacctt gaccagatta aataaagggc aagccattac tgttcacggt gctggtagga
    33601 cagatagtgg ggttcatgcc ctgggacagg tcattcattt tgacctgcct tatcagattg
    33661 atgaggagaa actccgtttt gccttggata cccagtctcc tgaagatatt gatgtgattt
    33721 cgattgagct tgtggcagat gattttcatt gccgttatgc caagcatagc aagacctatg
    33781 agtttattgt ggatagagga cgtcccaaaa atccgatgcg ccgtcactat gccacccact
    33841 ttccctaccc actcgatgtg gaacgaatgc agattgcaat caaaaagcta gagggaaccc
    33901 atgattttac cggttttaca gcctctggga ctagtgtaga ggataaggtt cgcaccatca
    33961 cagaagctag tttaatagtc gatgagacag gacaattttt gacctttacc ttttccggaa
    34021 atggtttctt gtataaacag attcgcaata tggtggggac tcttctcaaa atcggtaaca
    34081 accgcatgcc agtagagcag attgatctca tcttggagaa gaaggacagg caacttgcag
    34141 gtcccactgc agcaccaaat ggtttgtatt taaaggagat tcgttatgaa gaataatcgt
    34201 attttagcac tttctggaaa tgatattttt agtggtggtg gactgtcagc tgatttggct
    34261 acctatacct tgaacggctt gcatgggttt gtagcagtga cttgtttgac agccttgaca
    34321 gaaaaaggat ttgaagtctt tccaactgat gataccattt ttcaacatga attagatagc
    34381 ttgcgtgatg tggaatttgg gggaattaag attggtcttc tccctactgt cagtgtggct
    34441 gagaaggcct tggactttat caaacaacgc ccaggagtac ctgtggtgtt ggatcctgtc
    34501 ttggtctgca aggaaacgca tgatgtagct gtcagtgagc tctgccaaga gttgattcgc
    34561 tttttccctt atgtcagtgt gattacgcct aatctcccag aagcagaatt attatccggt
    34621 caggaaatta aaaccttgga agacatgaaa actgcagcgc agaaattgca tgatttagga
    34681 gcgccagcag tcattatcaa gggaggcaat cgtcttagtc aggacaaggc tgtggatgtc
    34741 ttttatgatg gacagacctt tactatccta gaaaatccag ttatccaagg ccaaaatgct
    34801 ggtgcaggtt gtacctttgc ctctagcatt gccagtcact tgattaaagg tgataaactt
    34861 ttgccagcag tagaaagctc taaggctttc gtttatcgtg ctattgcaca agcagatcag
    34921 tatggagtaa gacaatatga agcaaacaaa aacaactaaa atcgcccttg tatccctatt
    34981 aaccgccctt tctgtggttc taggttattt cttaaaaatc ccaacaccta caggaattct
    35041 aactctttta gatgctggtg tcttctttgc ggccttttac tttggtagtc gtgaaggagc
    35101 ggtagtcgga ggactagcaa gtttcttgat tgacctctta tcaggctacc ctcagtggat
    35161 gttctttagc ttggtcaacc atggcttgca gggatttttc gcaggattta aaggaaaaag
    35221 tcagtggtta ggccttattt tagcaactat tgccatggta ggaggctacg ccttgggttc
    35281 tactttgatg aatggctggg cagcagccct cccagaaatt ctaccaaatt tcatgcaaaa
    35341 tatggtaggg atgattgtag gatttattct tagtcaaagt atcaagaaga ttaagtaata
    35401 ctcttcgaaa atctcttcaa accacgtcag tttccatctg caacctcaaa acactgtttt
    35461 gagcagcctg cggctagctt tctagtctga ttttcattga gcataaagag gttgagtaaa
    35521 aagtctttca aataagaaaa acgcatagta tcaggtgttg aatgaacacc tgatactatg
    35581 cgttttattg tgggaagatt ttcctcattt tctagtgaaa ttgaactttt tctaacatct
    35641 tttgatagat gacttcttaa tcaattatca tttccattgt tgtttcttgg ggtttcatcc
    35701 cttgtctttg ataaaagcgg agagcaccct cgttatcatt ccagacactt agggttagat
    35761 tattgcagcc aatttcttta gcataatagc agaaaaatga caataaaata gcccctagat
    35821 attttgaaag gaaagcatag gggctatata gtttaatcaa aataaagcaa atctttgctg
    35881 gtgcttgtaa agaggtcgaa gccatccttg gtaacaacac cgcagtcttc aatacgaaca
    35941 ccgactttac cagggatata gatacctggt tcaacagaga agcacatgcc ttcttcgatg
    36001 accatgtcgt ttccttccat gatagatggg aattcatgga catccatacc gataccatgc
    36061 ccgagacggt ggttgaagta ctcaccataa ccagcttttt cgatgacctc acgggcagcg
    36121 cggtccactt catgagcagt cacacctggc ttgataaagt caagagcagc ttgttgggct
    36181 tcaagagtca agttgtaaat atctttcttg aattggtctg gtttgccgac agcgactgta
    36241 cgagtcatat ctgacgcata gccattgacc agaacaccca ggtcaaagag gagaagagca
    36301 tcattttcaa ccttattagc tgctggaatg ccgtgtggat tcgcagcatt atcaccagtc
    36361 aagaccatgg tatcaaagct catttcgtaa ccttcacgtt tcatggcaaa gtcaatttgt
    36421 gcgatgatat ctgtctcagt cttatcaaga gaaatattgt caaaaccaac atgaacagcc
    36481 ttgtcagcat aaagacctgc aaccatcatt ttttgcactt catcagctga tttgatgagg
    36541 cgcatgcgtt ggatacgagg agtgaggttg tcaaactcag cagtctcaaa aactgttttc
    36601 aaaccatggt atttggtcaa gatgagattg tcaaactcaa cagcgacacg tttgaagtca
    36661 agttgtggaa gagcatgttt gattttttgc catggatttt cagaatcgac atagcccact
    36721 actgggaagg aaacggtgct acttgcacgt tctacttcaa gagctgggac aaagaggaga
    36781 ggttcctgat ctgctaggac aaagaggaac atttggcgtt catggggatc actgtaaaaa
    36841 ccagtgaggt aattgattgt gacggggtca gatacgacag cgacgtctag tttttctgat
    36901 tcaagatatg ttaggatttg ttgtaattta gacatatgct accttctttc tatccctcta
    36961 ttttggcaaa aaatgagaga aaatgcaagg gagaagggta aaagaaaaga caaaaaactc
    37021 cgacaagata acggagttct ttgatatcat tttcctttgt ttaagaaaag tcagccttgc
    37081 ttttgacgtc actgtactct tcagcgattt cttggctgag gatatcctca taggcaggga
    37141 gttggatgtc ctgaccatat ttcttcttga gggcagtagt aactaggcga taagccagat
    37201 tggcattacg agagaggata ggcccgtgga agtaggaacc aaagacattc ttataatgaa
    37261 ccccttcacc gaccttttct tcgttgtttc catttccata gacaacctgt cccagcggtt
    37321 tttggtcatc agagaggaag gtacggcctt ggtgattttc aaatccatag taggtttcat
    37381 cgaaatcttc attgtgaatc ttgatgtcac cgataaaacg gttattggtc tggttgagcg
    37441 tgtagtgtcc catgacccct agcccttcga tacgttttcc tgaagcttca acataatatt
    37501 gacccaatag ttggaaacca ccgcagatag ccagaactac accgtcgttt tggatgtagt
    37561 tgtcaatgct ctctttttta gcaggtaggt cgtctgcaat gatactttgt tcaaagtctt
    37621 gaccaccacc gaaaaaggcg atgtcgtagt gattttcatc aaagtcatca tggagagaaa
    37681 cgatgtcaac ggtcacatgg gctcccagtt tttcagccac atacttgagc atgaggatgt
    37741 ttccattgtc cccgtaggta ttcatgagat ttccgtagag gtgggcaatg ttgagctgat
    37801 aggggtaatt gccatctttt gaggaaagtg aagtataaac cattagttca tctcctttct
    37861 aacaatctga cgactagcca gcagttcacg aaattccagc atggcagtat aagttgccag
    37921 aatataggca tgcttgcagt cttgattctc aatggtcttg agaacttgct ccagattact
    37981 cgtttcagtg attttctcag ctggatagcc agtcactcgg aggcgacgag cgatttcaga
    38041 atgacgaaca ccgccagcgt tgatttcagg aatgtccatg tcagtgattt gttcaaagtc
    38101 tgcatcccag atccagctag tgtcaattcc atctgcatag ttggcattaa ggaggacaga
    38161 taggctaaat ggataaggtg ctagtttgat catttcgata gcttgggttg caccgactgg
    38221 atttttaatc aagacaaggg tacattcctt gtcaccgata tgaaaggttt cttggcgtcc
    38281 aaagacagca cggctcttgt caaatccctg tttgatgagt tgcgaatcgg cacctaggaa
    38341 acgggcgatg gccacagcag ctagggcgtt atagatatta tagagcccgc cgatttggat
    38401 accgtattct tggacgtcta tgacaaagcg agagcgattg ttggtcaact caaccagttt
    38461 tgtcaaacga tagtcgagat caggacgttt acatccacaa ccttcacaga tataggtacc
    38521 caagtttgca taggtattat gctcatattt gaggatgcct tggcagtcag gacagagaat
    38581 cccttcggta ttgtagtgag ccagttgggc tggtcctttt tccaagtcaa aaccaaaata
    38641 ctctataggg tttggaatag ttggcttgta gaaaagtgga ctgtctccgt taaggagaac
    38701 agtagcagtt ggaactttcc gaatggcatc caatatcatg ttataggtag tatagatttc
    38761 accgaaacgg tccatctggt cacggaagat attagtaatg acaaaaagac taggctggat
    38821 atagtcacag atacgagata gactggcttc gtcaatttcg aggacggcaa tattttttcc
    38881 agttttagaa gatttggctg ttaggaaggt tgttgcaatc cctgtaatca tgttggcacc
    38941 gcttggattg gttagaactt gaccatagac ctcttttaaa atgccgacag tgagggcagt
    39001 tgtcagggtt tttccatttg ttccagtgac aacgacaatc tcgtagttct tagctaggtt
    39061 ttgtaaaata tctttatcaa attgaagggc gactttacct gggagcgtac ttccacggcc
    39121 aagacggctt aaaatgaagt gggaagaacg cccagcaaga aggcccaaag tagtttttaa
    39181 gttcatgttt ctattatatc ataaaagagg agaaagacag atttgagatt ccgcagaagt
    39241 aaaaacagcc caaagagggc tgttcgatac gattaaatct caacttaaat cgcaaacaag
    39301 tcattcaagt tctcagccaa ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa
    39361 ggaatcttgt tgtccatagc aacagtgatg atgttgatga tttcttgaga accttctgag
    39421 aagatgcttg ctccaagaat ttcttttgtt tcagtattga caacagcttt gaaggcaccg
    39481 cgaaggtctc catttacgtg accacgaggc atggctgcaa cggggatttc cttaacagcg
    39541 tatggaagtt tcaaatcagc tgcttggctt tcagtcaaac caacttgtga aagtgcaggt
    39601 gtgatgaaca tagtatttgg cacattgaga cggtcttcaa gtgtgtagct gccatctcca
    39661 gcaaggtagc tgtaaacaac acggaagtca tcaagtgaaa tgtaagtaaa ttgaaggcca
    39721 ccgttgatat ctccaactgc aaagacacca ggaacgtttg tttgacagtg tttgtctact
    39781 ttaatagcac cacgttcagt tagttcaata tctgtatttt caagttgaag tggttctaca
    39841 tttggtttgc gtccagttgc gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt
    39901 acgacaagca cttggtcacc atcgtttttg atttcagtag tatggatatt ttgaagcaat
    39961 tcaatgccat cttcttccat gtattgttta gcaagagctg cgatggaagg ttctgcacga
    40021 ggaaggaaag tatccaaggc atctaggact gtgaccttgc ttccaagttt gttgtaaagg
    40081 ccggcaaatt caagaccgat atttccgcca ccaaggattc caagtttttc aggtaatttg
    40141 tccaagtttt ggatacctgt tgagtcaaag atgtttttgc ttgtagcaag tccagggatt
    40201 ggcaagacgt ttgaaacagc accagtgttg atgacgattg tttcagcagt cagttctttc
    40261 ttttcatcac cagcttggat ttcgatgact ttatttgaaa ggaagtgagc ttccgcatca
    40321 aagatatcta cgcctgtacc agcaacagtc gcatagtttt taccgttgag gcgaccagtg
    40381 atcgtgtttt tagtagcaat cacttcttca aaagacaagt ccttttcagc agcaactagc
    40441 aaggttttag ttgggataca accaatgttg atacaagttc caccgtacat agccttgctg
    40501 cgttcaacga gggcaacttt tttgccagct gaagccaatt tacctgctag tgttttacca
    40561 gctttaccaa atccaataac aattaaatcg tatgttaaca tttatcttcc tcctattcga
    40621 atttcattct agcacaagaa aaaaactttt gcacaattct gctacggaaa agacagaaaa
    40681 agataaaaac gctttccaga caaaaaacta gtaatttttt gacaagcttg ataaactaaa
    40741 atcctatcta tttttatata agatagaata aatgttcgaa aataggaggt tttatgaaat
    40801 cgaatcgtta tattattgcc tttgctgggg tcattttaca cttaatgctg ggttcgactt
    40861 atgcttggag tgtttatcgt aaccctatta ttgaaaaaac gggatgggat caggcttctg
    40921 ttgcctttgc ctttagtcta gcaatctttt gtttgggctt atcggctgca tttatggggc
    40981 gtttggtaga aaaatttggt ccgagagtca tggggagtct atctgctttt ctatacgcag
    41041 gtggaaatat cttaacagga tttgcaatag accgtcagga gctgtggttg ttgtatctcg
    41101 cttatggcat tttaggtggg cttggtttgg gagcaggcta tattacccct gtgtcgacga
    41161 ttataaaatg gtttcctgat aaacgtggtc tcgcaacagg tttagcgatt atggggtttg
    41221 gttttgcttc tttattgact agtcccatag cgcaacacct catcgcaggg gtagggcttg
    41281 tagaaacttt ttatatttta ggagcaagtt actttattat catgctccta gcttcacaat
    41341 tcattaagcg tccaaatgag caagagcttg caattttatc ttcttcaggg aaagaaaaaa
    41401 cagcctcttt gacgcaagga atggctgcaa atcaggctct aaaaagcaat cggttttata
    41461 tactttggat tattttcttt atcaacatag cttgtggttt aggcttaatt tcagcggcat
    41521 cgccaatggc acaggagatg gctggcttgt ctacaagtca tgcagcagta atggtgggcg
    41581 ttttagggat tttcaatgga tttggtcgct tgctctgggc gagcttatct gactatatcg
    41641 gtcgccctct aacctttagt atattactgc ttgttaatct tttcttttct ctctcacttt
    41701 ggctctttac agattccgtt ttatttgtag tcgctatgtc tattttgatg acttgctatg
    41761 gagctggttt ttcattgatc ccagcttatc tcagtgatat ttttggaacc aaggaattgg
    41821 ccgctctgca tggatatatt ttaacagctt gggcaatggc tggtttagcg ggacctattt
    41881 tattagcaga gacttataaa atggctcatt cgtacacaca aaccttgttc gtttttctca
    41941 ttttatacag tatcgccttg gctttgtctt attatctagg tcgttcaatc aaaaaagaaa
    42001 gtcaaaaacc gcttacatga ttttctgtag aaatggggct atcttttgct ctggtcttgt
    42061 gttatactag ataggttgca aagaaaacag tacttttctt ttgtggaaaa gaagcaacac
    42121 gattttatat ctagttatat gaaaatacgt cataaaaaga aaagtataaa ctaagcccta
    42181 tagggtggct tcgcaccacc tttagaaaga agaataacgt gaaatttaat gaattaaact
    42241 tgtctgctga tttgctggca gaaattgaaa aagctggttt tgtagaagct agtcctatcc
    42301 aagaacaaac tattcctttg gcccttgaag gcaaggatgt tatcggtcaa gctcagactg
    42361 gtacaggaaa aactgcagcc tttggcttgc ctacccttga aaaaatccgt acagaagaag
    42421 cgactatcca agccttggtc atcgctccaa ctcgtgaact agctgtccaa agtcaagaag
    42481 aactcttccg ctttggtcgt agtaagggag tcaaagtccg ttcagtatat ggcggatcaa
    42541 gcattgaaaa acaaattaag gctcttaaat ctggtgccca tatcgtggtg ggaactccag
    42601 gtcgcctctt ggacttgatt aaacgcaagg ccttgaaatt acaaaacatt gaaaccctta
    42661 tccttgacga agcggatgaa atgcttaaca tgggcttcct tgaagacatc gaagccatta
    42721 tttcccgtgt acctgagaac cgtcaaactt tgcttttctc agcaactatg ccagatgcca
    42781 tcaaacgtat cggtgttcag tttatgaaag cccctgaaca tgtcaagatt gcagctaagg
    42841 aattgacaac agaattggtt gaccagtact atatccgtgt taaggaacaa gaaaaatttg
    42901 acaccatgac tcgtctcatg gatgtggcac aaccagaact cgctattgta tttggtcgta
    42961 ccaaacgccg tgtggatgaa ttgactcgtg gtttgaaaat tcgtggcttc cgtgcagaag
    43021 gaattcatgg cgacctagac caaaacaaac gtcttcgtgt ccttcgtgac tttaaaaatg
    43081 gcaatcttga tgttttggtt gcgacagacg ttgcagcgcg tggtttggat atttcaggtg
    43141 tgacccatgt ctacaactac gatattccac aagatcctga gagttatgtt caccgtatcg
    43201 gtcgtacagg tcgtgctggt aagtcaggtc aatctattac ttttgttgct ccaaacgaaa
    43261 tgggttacct tcaaatcatt gaaaacttga ctaagaaacg catgaaaggt ctcaaacctg
    43321 caagtgtaga agaatccttc caatcaaaaa aacaggtagc tctcaagaaa atcgaacgtg
    43381 attttgcaga tgaaaccatt cgtgccaact ttgagaaatt tggtaaggat gctcgcaaat
    43441 tggctgctga gtttactcca gaagaattgg caatgtatat cttgagtctg acagtccaag
    43501 acccagatag ccttccagaa gtggagattg cacgtgaaaa accactaccg tttaaaccat
    43561 caggtaatgg tttcggtggt aaagctaagg gaggtcgtgg aggccgtcgt ggggacgacc
    43621 gtcgagagcg tgatcgccgt ggcaatggtc gccgtgatga gttcaaaaaa ggaagtcgtg
    43681 gcaacgatcg ttttgataag gaaaaacgtt accgtaagga taataaaaaa ccacgcaata
    43741 ctttaagcga aaagcaaaca ggctttgtta ttcgtaacaa aggcgataaa taagaaaaag
    43801 cggttcaaaa tgaatcgctt ttttatataa ggagaccgaa tgtgaaaaga caaataaaat
    43861 tattatatta tcttttataa tgttatgata cacaaaaata ggcaacttgt caatagaaaa
    43921 ggaacaattt aattaaaaat attctggttt ttaattaaat tgtaagtcaa tgcaagtcaa
    43981 taaggaattt tcagataatt attgaaaaag agacgttttt atgttataat gatagcgatt
    44041 aaattcgagg tgaaaaatgg cacatttatt agaaaaaact agaaaaatta catcaatttt
    44101 gaaacgctca gaggagcagt tgcaggatga gctcccttac aacgctatta cgcgtcagtt
    44161 agcggatatt attcattgca atgcctgcat tatcaatagt aagggacgtc tgcttggcta
    44221 ttttatgcgt tataaaacaa atacagatcg cgtagagcaa ttcttccaaa ctaagatttt
    44281 cccagatgac tacgttcaag gggctaatat gatttacgaa acagaagcaa acttgcctgt
    44341 tgagcatgat atgagtattt tccctgttga gagtagagat gattttccag atggcttgac
    44401 gactattgca ccgattcatg tatcggggat tcgccttggt tctttgatta tttggcgtaa
    44461 tgataaaaaa ttcgaagatg aggacttggt tcttgttgag attgccagta ccgttgttgg
    44521 gattcagctt cttaacttcc aacgtgaaga agatgagaaa aatattcgtc gtcgtactgc
    44581 tgtcaccatg gcggtcaata ccctttctta ctccgaactc cgtgctgttt cagcaatttt
    44641 aggggaatta aatggaaatg aagggcagtt gactgcgtca gtgattgcag atcgtatcgg
    44701 aatcactcgc tctgtgattg tcaatgctct tcgtaaactt gagtctgcgg ggattattga
    44761 aagtcgctca cttggaatga agggaaccta tcttaaggtc ttgatttcag atatttttga
    44821 agaagtgaag aaaagagatt actaatgaca aaggctttaa tttcgattga ttatacagaa
    44881 gattttgttg ctgatagtgg gaagctgaca gcaggtgctc cagctcaggc gatttcggat
    44941 gccattagca aggtaactcg attagctttt gaacgaggag attatatctt ctttactatt
    45001 gatgctcatg aagaaaacga ttttttccat ccggaaagta agttatttcc tcctcataat
    45061 ctgattggga cgagtggacg gaatttatat ggagatttgg ggatctttta tcaagagcat
    45121 ggttcagaca gtcgtgtctt ttggatggat aaacgccatt actcagcttt ttcagggact
    45181 gacctagata ttcgtttgag agagcgtaga gtgtctaccg ttatcttaac aggtgtcttg
    45241 acggatatct gtgtcctaca tacagctata gatgcctata atctaggata tgacatcgag
    45301 attgttaaac cagctgttgc ttccatctgg cctgaaaatc atcaatttgc cctaggtcat
    45361 ttcaaaaata cactcggagc taagttagta gatgaaaatc taaatgaact ttctgagtaa
    45421 cctggttggt gaaaatgaaa aaatttgaaa aaaagtgttg acaagcatcc caaaagttga
    45481 tatactagta aagtaatcga tgcggggatg gcggaattgg cagacgcgca ggactaagga
    45541 tcctgtgacc gctttaggtc gtgagggttc aagtccctct ctccgcatag gaaaagagtt
    45601 agctaaagct agctcttttg tatttctcta aaaaattaga atccagtcaa atgtagtgga
    45661 gaatcagcga aaatatggga aaactgatcc ttgtttctgc ttttgtcata gaagaataat
    45721 aaagattatc ttcttcaaat tctccgatat tctctaaagt tttgtgcaag ttgcacagaa
    45781 cttttttatt tttttggtca tcttgccata gaaatataaa gcgttttcat atataatata
    45841 attatcaaaa gacaaaagga gttcacctca tggtagaatt gaatcttaaa aatatttaca
    45901 aaaaatatcc aaacagcgaa cactattcag ttgaagattt caacttgaac ataaaagata
    45961 aagaatttat cgttttcgta ggaccttcag gatgtggtaa atcaactaca ctccgtatga
    46021 ttgctggtct tgaagacatt acagaaggta ctgcatctat cgatggcgtg gttgtcaacg
    46081 acgtagctcc aaaagaccgt gatatcgcca tggtattcca aaactacgct ctttacccac
    46141 acatgactgt ttatgacaac atggctttcg gcttgaaatt gcgtaaatac agcaaagaag
    46201 acattaacaa acgtgttcaa gaagcagctg aaatacttgg attgaaagaa ttcttggaac
    46261 gtaaaccagc tgacctttca ggtggtcaac gtcaacgtgt tgccatgggg cgtgcgattg
    46321 tccgtgatgc gaaagtattc ttgatggacg aacctttgtc aaacttggat gccaaacttc
    46381 gtgtatcaat gcgtgctgaa atcgctaaaa ttcaccgtcg tatcggagct acaactatct
    46441 atgtaactca cgaccaaaca gaagcgatga cacttgcaga ccgtatcgtt attatgtcag
    46501 ctactaagaa ccctgctggt acaggtacta tcggacgtgt agaacaaatc ggtactcctc
    46561 aagaagttta caaaaatcca gttaacaaat tcgttgcagg attcatcgga agcccagcta
    46621 tgaacttcat caccgtgaaa ttggttggta gcgaaattgt ttctgacggt ttccgtttga
    46681 aagtgccaga aggagcattg aaagttcttc gtgaaaaagg ctacgaagga aaagaattga
    46741 tctttggtat ccgtccagaa gacgtgaatg cagaacctgc tttccttgaa acattcccag
    46801 actgtgttgt aaaagcgact atctctgtat cagaactgct tggttcagaa tctcaccttt
    46861 actgccaagt tggtaaagac gagtttgttg caaaagttga tgctcgtgac tacttgcaaa
    46921 caggtgcaac agttgagctt ggatttgact tgaacaaagc acacttcttc gatgtagaaa
    46981 ctgaaaaaac aatctactaa aataaataaa attcaaagca ctacaagaaa agatatctct
    47041 ttatcaattg tagtggagag atatcagtta atctagggag agaaacaaaa tgcttctctc
    47101 ctttttgcta gggaagtcat attatgcatc tatattgtga tgctctttat actcttcgaa
    47161 aatctcttca aaccacgtca acgtcgcctt gccgtacgta tgattactga tttcgtcagt
    47221 tttatctgca acctcaaaga tgtactttga gcagcttacg gctagtttcc tagtttgctc
    47281 tttgatttcc attgagtatt attgtgagta ccatctacaa gtgaagctat atgcgtaaac
    47341 tacgtgagca attgaattcg aactagagag gtaataataa atttatgcta tagttatggt
    47401 gacttgtatg cttttgattc tagtttatca aataatagat tagaattgtc agataatatc
    47461 attttgtgtt ataatgaaga aaaaacagag gtgttcaaat gtcagaagca ggtcataagt
    47521 ttttagcaaa attggggaaa aaacgcttac gtccaggtgg aaagcgtgcc acagattggt
    47581 taattgcaga aggaggattt tcaaaataaa agagaatact agaggttgcg tgtaataggg
    47641 gaactacagc aattgagttg gcacagcgtt ttggttgcaa gataactgct gttgatatgg
    47701 atgctcaagc tttagaagtg gctaaaaaat ctgctggaac ggcaggtgtt gctcatttaa
    47761 tcagttttga aagagcaaat gcaatgaaac ttccttatca agatgctagt tttgatattg
    47821 ttataaatga agctatgctg actatgcaag ccgatcaagc taagaaaaaa tgtgtaatgg
    47881 aatatctaag ggtattaaaa cctggaggtc ttctcttgac acatgatgtg cttcttaagg
    47941 aagctaaaga gtctatcaga caggaattat cacaagcaat tcatgtaaat gtaggtcctt
    48001 taactcaaga tggttgggaa caggtgatga tagaatcagg ttattgtgat gtgaaagcat
    48061 tgactggtga aatgacatta atgaaattat cgggtatgat ttatgacgaa ggtttgctag
    48121 gaactttgaa aatttgtgta aatgcttgta aaaaggagaa tagaaagcag tttttaacta
    48181 tgtataaaat gtttgctaag aataaacaga aattgggctt tattgcgatg gctagttata
    48241 aatcgtcaaa acgttagata attattgaag ttaacttttc cttttttctt tcttaaaaaa
    48301 tatgctataa tagagagtaa aaaactttga aagaaagaaa aaagatgaat ttaaaagatt
    48361 acattgcaac aattgaaaat tatccaaagg aaggcattac cttccgtgat attagtcctt
    48421 tgatggctga tggaaatgct tatagctacg ctgttcgtga aatcgttcag tatgctactg
    48481 acaagaaagt cgacatgatc gtgggacctg aagctcgtgg atttatcgtg ggttgtccag
    48541 ttgcctttga gttgggaatt ggttttgcgc ctgttcgtaa gccaggtaaa ttgccacgtg
    48601 aagttatttc tgctgactat gaaaaagagt acggtgtcga taccttgact atgcacgcgg
    48661 atgccattaa gccaggtcaa cgtgttctta ttgtagatga ccttttggcg acaggtggaa
    48721 ctgttaaggc aactatcgag atgattgaaa aacttggtgg tgttatggca ggttgtgcct
    48781 tccttgttga attggatgaa ttgaacggcc gtgaaaaaat tggtgactac gactacaaag
    48841 ttcttatgca ttattaatga aaacagtccc tagggctgtt ttctctacac taggatataa
    48901 aaatagacta taactagtta gagaaaaact ataattgaaa actatatctt cttgcagtat
    48961 aataaaagga ctaagtgttt gagatttgtc ttcaaacata tgcaattatt cctgaaagag
    49021 tacagttagg agagggttat gccgattcga attgataaaa aattgccagc tgttgagatt
    49081 ttacggacag agaatatctt tgtcatggat gatcaacgtg ctgcccacca agatatccgt
    49141 cctttgaaga ttttaatttt aaatctcatg ccacagaaaa tggtcacaga gacccagttg
    49201 ttgcgccact tggctaatac acccctacaa ctggatattg attttctcta tatggagagc
    49261 caccgttcta aaacaactcg ttcagagcac atggagacct tctataaaac ttttcctgaa
    49321 gtcaaggatg agtattttga tgggatgatc atcacgggtg ctccagttga gcatttacca
    49381 tttgaggaag tggactattg ggaggaattt agacagatgc ttgagtggtc taagactcat
    49441 gtctattcga cccttcatat ctgttggggg gctcaggctg ggctttatct gcgctatggt
    49501 gtagaaaaat accagatgga cagtaagcta tcaggtattt atcctcagga caccctaaaa
    49561 gagggtcacc ttctatttag aggctttgat gatagctatg tatcccctca ttcacggcac
    49621 acggagattt ctaaggaaga ggtcttaaac aagaccaatc tcgagatttt atcagaagga
    49681 cctcaggttg gggtttctat tttggccagt cgtgatttac gagaaattta tagttttggt
    49741 catttggagt atgaccgtga taccttggca aaagagtatt ttcgagatcg tgatgcaggt
    49801 ttcgacccac atattccaga aaattacttt aaagatgatg atgttaatca ggtaccttgt
    49861 ctttgttggt cttcatctgc agccctcttt ttcagtaatt gggtaaacca tgcggtctat
    49921 caggagacgc cttttgattg gagaaagata gaagatgatg catctgcata tgggtattta
    49981 taagaggaat tatgacatat ttagacgctt ttaaatcagg taccttggtt ttaccgagtg
    50041 ccctgctctt gcattttaag gaactctttc cttctagcga cgattttctg gtttggcaat
    50101 ttttctattt gcaaaatacg acaggcttag aagaaatgtc gccaagccag attgctgaaa
    50161 ggattggcaa ggaaatttcg gatgtcaacc agtccatttc taatctgacg gaaaggggac
    50221 tgctccagta tcgtactatc gaattaaatg gcgaaattga attgctcttt gatgctagtt
    50281 tggccttgga acgtttggat gacctgtttg gagcagttca ttcaagttca gaccagctaa
    50341 cacctcaaaa ccagctcaag gatttggtgg aaaccttcca gcaggagttg ggacgattgt
    50401 tgacgccttt tgagattgag gatttgacca agacactaaa ggaagatgga accagtgctg
    50461 acttgattaa ggaggctctt cgtgaagctg ttttgaatgg aaaaccaaac tggaagtaca
    50521 ttcaggctat tttgagaaac tggcgccatg aaggaatcaa gagtgtggct caaattgagg
    50581 ccaagagagc agaaagagaa gcaagcaatc ctcagttgac acaggtatct gcagatttca
    50641 gaaatgccat ggatctctgg aaggattaat ccatgcaagt aggcttgaaa tccgagtaag
    50701 atttgcaagc tgtgtataat tgtgatagaa taaatagaaa ataaattgaa aaaagaggta
    50761 tgtgaaatgt cacgtaaacc atttatcgct ggtaactgga aaatgaacaa aaatccagaa
    50821 gaagctaaag cattcgttga agcagttgca tcaaaacttc cttcatcaga tcttgttgaa
    50881 gcaggtatcg ctgctccagc tcttgatttg acaactgttc ttgctgttgc aaaaggctca
    50941 aaccttaaag ttgctgctca aaactgctac tttgaaaatg caggtgcttt cactggtgaa
    51001 actagcccac aagttttgaa agaaatcggt actgactacg ttgttatcgg tcactcagaa
    51061 cgccgtgact acttccatga aactgacgaa gatatcaaca aaaaagcaaa agcaatcttt
    51121 gcgaacggta tgcttccaat catctgttgt ggtgaatcac ttgaaactta cgaagctggt
    51181 aaagctgctg aattcgtagg tgctcaagta tctgctgcat tggctggatt gactgctgaa
    51241 caagttgctg cctcagttat cgcttatgag ccaatctggg ctatcggtac tggtaaatca
    51301 gcttcacaag acgatgcaca aaaaatgtgt aaagttgttc gtgacgttgt agctgctgac
    51361 tttggtcaag aagtcgcaga caaagttcgt gttcaatacg gtggttctgt taaacctgaa
    51421 aatgttgctt catacatggc ttgcccagac gttgacggtg cccttgtagg tggtgcgtca
    51481 cttgaagctg aaagcttctt ggctttgctt gactttgtaa aataatcagt aagtagcaaa
    51541 agctaggtgg aacagcattc agatgtctgt tacatttttt ataggagagc aaagattgaa
    51601 aacaaaaatt ggattagcaa gtatctgttt actaggcttg gcaactagtc atgtcgctgc
    51661 aaatgaaact gaagtagcaa aaacttcgca ggatacaacg acagcttcaa gtagttcaga
    51721 gcaaaatcag tcttctaata aaacgcaaac gagcgcagaa gtacagacta atgctgctgc
    51781 ccactgggat ggggattatt atgtaaagga tgatggttct aaagctcaaa gtgaatggat
    51841 ttttgacaac tactataagg cttggtttta tattaattca gatggtcgtt actcgcagaa
    51901 tgaatggcat ggaaattact acctgaaatc aggtggatat atggcccaaa acgagtggat
    51961 ctatgacagt aattacaaga gttggtttta tctcaagtca gatggggctt atgctcatca
    52021 agaatggcaa ttgattggaa ataagtggta ctacttcaag aagtggggtt acatggctaa
    52081 aagccaatgg caaggaagtt atttcttgaa tggtcaagga gctatgatgc aaaatgaatg
    52141 gctctatgat ccagcctatt ctgcttattt ttatctaaaa tccgatggaa cttatgctaa
    52201 ccaagagtgg caaaaagtgg gcggcaaatg gtactatttc aagaagtggg gctatatggc
    52261 tcggaatgag tggcaaggca actactattt gactggaagt ggtgctatgg cgactgacga
    52321 agtgattatg gatggtgctc gctatatctt tgcggcctct ggtgagctca aagaaaagaa
    52381 agatttgaat gtcggctggg ttcacagaaa cggtaaacgc tatttcttta ataatagaga
    52441 agaacaagtg ggaaccgaac atgctaagaa aatcattgat attagtgagc acaatggtcg
    52501 tatcaatgat tggaaaaagg ttattgatga gaacgaggtg gatggtgtca ttgttcgtct
    52561 aggttatagc ggtaaagaag acaaggaatt ggcgcataac attaaggagt taaaccgtct
    52621 gggaattcct tatggtgtct atctctatac ctatgctgaa aatgagaccg atgctgagaa
    52681 tgacgctaaa cagaccattg aacttataaa gaaatacaat atgaacctgt cttaccctat
    52741 ctattatgat gttgagaact gggaatatgt aaataagagc aagagagctc caagtgatac
    52801 agacacttgg gttaaaatca tcaacaagta catggacacg atgaagcagg cgggttatca
    52861 aaatgtgtat gtctatagct atcgtagttt attacagacg cgtttaaaac acccagatat
    52921 tttaaaacat gtaaactggg tagcggccta tacgaatgct ttagaatggg aaaaccctta
    52981 ttattcagga gaaaaaggtt ggcaatatac ctcttctgaa tacatgaaag gaatccaagg
    53041 gcgcgtagat gtcagcgttt ggtattaagc gatgatttga aagagggatg tgatagtagc
    53101 accctctttt tctttgtttt atgatagttc atcctcgagt aaattcaaga tcttgctcgg
    53161 aaatgaagct tatatagtag attgaatata gacaaatacc ttgtgattgg taaaacattt
    53221 tagaaattca tttacctttc ctaatcgact tggtttcatc ttatttcaat ctattatagt
    53281 attggggaat ttcttcaaac cacatcagct tggtcagttc tacctgcgac ctcaaaactt
    53341 gtgctttggt caagctgggt ttagtttcct agtttgctga tggatttcca ttgactataa
    53401 gcatccaacc ctctttttgt cttctaaaga attcttaaat tatcagtcta ttgcaacttt
    53461 tctcatataa gttctttgtc ttgctatttg ttttccttag tagtatacta aggtagtaat
    53521 cattaagaag tggttacaaa aaataatgaa tgaggtaaag aaaatggtag aattgaaaaa
    53581 agaagcagta aaagacgtaa catcattgac aaaagcagcg ccagtagcat tggcaaaaac
    53641 aaaggaagtc ttgaaccaag ctgttgctga tttgtatgta gctcacgttg ctttgcacca
    53701 agtgcactgg tatatgcatg gtcgtggttt ccttgtatgg catccaaaaa tggatgagta
    53761 catggaagct cttgacggtc aattggatga aatcagcgaa cgcttgatta cactcggtgg
    53821 aagcccattc tctacattga cagagttcct tcaaaatagt gaaatcgaag aagaagctgg
    53881 tgaataccgt aatgttgaag aaagcttgga acgtgttctt gttatctacc gttacttgtc
    53941 agaacttttc caaaaaggtt tggatgtcac tgatgaagaa ggtgacgatg tgacaaacgg
    54001 tatctttgca ggcgctaaaa ctgaaacaga taaaacaatt tggatgcttg cagccgaact
    54061 tggacaagca cctggtttgt aagaaataag gaaacataga taaatctgta ggaaatttct
    54121 tacagatttt ttctattttg taagctattc caaaccagtc tttttttgag ttttttgata
    54181 aaatagtact atcagtgaaa aggatggaag catgactaag aaaatcgtag ctatttgggc
    54241 ccaggatgaa gagggtgtga ttggtaagga aaatcgtctg ccttggcatt tgccagcaga
    54301 attgcagcac tttaaagaaa caactctgaa tcatgctatc ttgatggggc gtgtgacctt
    54361 tgatgggatg gggcgtcgct tgcttccaaa acgggaaacc ctgattttga cgcgtaatcc
    54421 ggaagaaaag atagatgggg ttgctacttt tcaggacgtc cagtctgttc ttgactggta
    54481 tcaggatcaa gaaaagaatc tctacattat cggtgggaag caaatttttc aggcttttga
    54541 accttacctt gatgaagtga ttgtcactca cattcatgct cgggtggaag gagataccta
    54601 tttccctgaa gagcttgact tgtctctttt tgagacagtt tcaagcaaat tttacgccaa
    54661 agatgagaag aatccttatg attttaccat ccaataccgc aagagaaagg aagtataatg
    54721 gaacggagtt tatttggtct attcacggct tttctgtgtt tcatctgctt tctggcagga
    54781 gcacaggctt ttcgtaaaaa gcgttacggg ctttctatcc tcctttggtt aaatgccttt
    54841 accaatctgg tcaatagtat tcatgctttt tacatgacct tattttagat agaatgacag
    54901 tatagaatgg aaggaaatca tgtctacaaa tagaaaaaat gatatgatgg tttattgctc
    54961 attttgtggc aaaaaccaag aagaagtaca aaaaataatt gctggcaaca atgcttttat
    55021 ttgtaatgaa tgcgtggagt tagctcagga aatcattcga gaagaattgg ttgaggaagt
    55081 cttggcagac ttgtctgagg tgccaaaacc aattgaactc ctccatatct tgaaccacta
    55141 tgtaattggt caagatcgtg ccaagcgtgc cttggcagtg gcggtttata accactacaa
    55201 acgcatcaat ttccacgata cacgcgaaga gtcagaagat gtggatttgc agaagtcaaa
    55261 cattttgatg attggcccaa ctggttcagg gaaaactttc cttgcccaga ccttggctaa
    55321 gagcttgaat gtgccttttg ctattgcgga tgcgacagct ctgacggagg ctggttatgt
    55381 gggtgaggac gtggaaaata tcctcctcaa actcttgcag gttgctgact ttaacatcga
    55441 acgtgcagag cgtggcatta tctatgtgga tgaaattgac aagattgcca agaagagtga
    55501 gaatgtgtct atcacacgtg atgtttctgg tgaaggggtg caacaagccc ttctcaagat
    55561 tatcgaggga actgttgcta gcgtgccgcc tcaaggtgga cgcaaacatc cacaacaaga
    55621 gatgattcaa gtggatacaa aaaatatcct cttcatcgtg ggtggtgctt ttgatggtat
    55681 tgaagaaatt gtcaaacaac gtctgggtga aaaagtcatc ggatttggtc aaaataataa
    55741 ggcgattgac gaaaacagct catatatgca agaaatcatc gctgaagaca ttcaaaaatt
    55801 tggtattatc cctgagttga ttggacgctt gcctgttttt gcggctcttg agcaattgac
    55861 cgttgatgac ttggttcgca tcttgaaaga gccaagaaat gccttggtga aacaatacca
    55921 aactttgctt tcttatgatg atgttgagtt ggaatttgac gacgaagccc ttcaagagat
    55981 tgctaataaa gcaatcgaac ggaagacagg ggcgcgtgga cttcgctcca ttatcgaaga
    56041 aaccatgcta gatgttatgt ttgaggtgcc gagtcaggaa aatgtgaaat tggttcgcat
    56101 cactaaagaa actgtcgatg gaacggataa accgatccta gaaacagcct agaggtgact
    56161 atggaactta atacacacaa tgctgaaatc ttgctcagtg cagctaataa gtcccactat
    56221 ccgcaggatg aactgccaga gattgcccta gcagggcgtt caaatgttgg taaatccagc
    56281 tttatcaaca ctatgttgaa ccgtaagaat ctcgcccgta catcaggaaa acctggtaaa
    56341 acccagctcc tgaacttttt taacattgat gacaagatgc gctttgtgga tgtgcctggt
    56401 tatggctatg ctcgtgtttc taaaaaggaa cgtgaaaagt gggggtgcat gattgaggag
    56461 tacttaacga ctcgggaaaa tctccgtgcg gttgtcagtc tagttgacct tcgtcatgac
    56521 ccgtcagcag atgatgtgca gatgtacgaa tttctcaagt attatgagat tccagtcatc
    56581 attgtggcga ccaaggcgga caagattcct cgtggtaaat ggaacaagca tgaatcagca
    56641 atcaaaaaga aattaaactt tgacccgagt gacgatttca tcctcttttc atctgtcagc
    56701 aaggcaggga tggatgaggc ttgggatgca atcttagaaa aattgtgagg aaaagaaaat
    56761 ggcaaaaaca attcatacag ataaggcccc aaaggctatc ggaccctatg ttcaaggaaa
    56821 aatcgttggc aaccttttgt ttgctagcgg tcaagttccc ctatcccctg aaactgggga
    56881 aattgtagga gagaatatcc aagaacagac agagcaagtc ttgaaaaaca tcggtgctat
    56941 tttggcagaa gcaggaacag actttgacca tgttgtcaaa acaacttgtt tcttgagcga
    57001 tatgaacgac tttgttcctt ttaatgaggt ttaccaaacg gccttcaaag aggaattccc
    57061 agctcgttca gctgtggagg tagctcgtct tcctcgtgat gtaaaagtcg aaattgaagt
    57121 catcgcagag attggataag ctagttgaag tttggtgttg ccaaacttct tttgatataa
    57181 ggagaaaaag atgacaaaga aacaacttca cttggtgatt gtgacaggga tgagtggcgc
    57241 agggaaaact gtagccattc agtccttcga ggatctaggt tatttcacca ttgataatat
    57301 gccgccagct ctcttgccta agtttttgca gctggttgaa attaaggaag acaatcctaa
    57361 gttggccttg gtagtggata tgcgtagccg ttctttcttt tcagagattc aagctgtttt
    57421 ggatgagttg gaaaatcaag atggtttgga tttcaaaatc ctctttttgg atgcggctga
    57481 taaggaattg gtcgctcgtt acaaggaaac cagacggagt cacccactag cagcagacgg
    57541 tcgtatttta gatggaatca agttggaacg tgaactcttg gcacctttga aaaatatgag
    57601 ccaaaatgtg gtggatacga ctgaactcac tccacgtgag ctgcgcaaaa cccttgcaga
    57661 gcagttttca gaccaagaac aagcccagtc tttccgtatc gaagtcatgt ctttcggatt
    57721 taagtatgga atcccgattg atgcggactt ggtctttgat gtccgtttct tgccaaatcc
    57781 ctattattta ccagaactga gaaaccaaac gggtgtggat gaacctgttt atgattatgt
    57841 catgaaccat cctgagtcag aagactttta tcaacattta ttggccttga ttgagccgat
    57901 tctgccaagt taccaaaagg aaggtaagtc cgttttgacc attgccatgg gatgtacggg
    57961 tggacaacac cgtagtgtgg catttgctaa acgcttggtg caggacttat ccaagaattg
    58021 gtctgttaat gaagggcatc gcgacaaaga ccgcagaaag gaaacggtaa accgttcatg
    58081 agaaaaccaa agataacggt gattggtgga gggactggaa ttcccgtcat tctaaaaagt
    58141 ctgcgggaaa aagatgtgga aatcgcagct atcgtgacgg tggcagatga tggtggttct
    58201 tcaggtgaac tccgaaaaaa tatgcaacag ttgacaccgc caggtgattt tcgtaatgtc
    58261 cttgtggcca tgtcggatat gcctaagttt tatgagaagg tctttcagta tcggttctct
    58321 gaggatgccg gagcctttgc tggccatcca ttgggaaatc tcatcattgc tggcttgtca
    58381 gaaatgcagg gttcaaccta taatgccatg cagttattga gcaaattttt ccatacaaca
    58441 gggaaaattt atccttccag tgaccatcct ttgacccttc atgcagtctt tcaggatggg
    58501 acagaagtgg ctggagagag tcatattgta gaccatcgag gcataattga caatgtctat
    58561 gtgaccaatg ccctaaacga tgatacgcct ctggccagcc gtcgagtagt gcagaccatc
    58621 cttgaaagtg acatgattgt cctagggcca ggttctctct ttacctctat tttgcccaat
    58681 atcgtgatta aggaaattgg gcgggctctt ttggaaacca aggcagaaat tgcctatgtc
    58741 tgcaatatca tgacccaacg tggggagacg gaacacttta cagatagcga ccacgtggaa
    58801 gtcttgcatc gtcaccttgg tcgccctttt atcgacactg tcttggtgaa tattgaaaaa
    58861 gtgcctcagg aatacatgaa ttccaaccgt tttgatgaat acttggtgca agtggaacac
    58921 gattttgtag gtctttgtaa gcaagtttcg cgcgtgattt catctaactt ccttcgtctg
    58981 gaaaatggcg gtgccttcca cgatggagat ttgattgtgg acgagttgat gcgcattata
    59041 caggtgaaaa aatgagtttc acagtagcag taaaagaaga aatcctgggc caacaccatc
    59101 tgagctggca tgaattatct gccattatca aaatgtctgg tagcatcggt ctctcgactt
    59161 cgggcttgac tttgtcagtt gtgacagaaa atgccaaact ggcccgtcac ctctatgagt
    59221 cctttctcca tttctatgaa atcaaatctg aaattcgtca ccaccaacgg agcaatcttc
    59281 gcaagaatcg cgtctatacc gtttttacag atgaaaaggt gcaggatttg ttaagtgatt
    59341 tgcacttggc agactctttc tttggcctgg aaacaggtat tgatgaggcg attttatcgg
    59401 atgaggaagc aggtcgtgcc tatctctgtg gcgctttctt ggcaaatggg agcattcgtg
    59461 accctgagtc aggcaagtac cagttggaaa tcagttctgt ttatctggac cacgcgcaag
    59521 gaattgcctc ccttctccaa cagtttttac tggatgccaa ggtgcttgag cgcaagaagg
    59581 gggctgtgac ctatctccag cgagcagaag acattatgga cttcttgata gtaatcggag
    59641 ccatgcaggc acgtgatgat tttgagcggg ttaagatttt gcgagaaacc cgtaacgacc
    59701 tcaatcgggc taataatgcc gagacagcta atatcgctcg gacagtttct gccagcatga
    59761 agaccattaa caatatcagt aaaataaaag atatcatggg cttagaaaat ttgccagtag
    59821 atttgcagga agtggcgcaa ctgaggattc agcacccaga ctactctatc caacagttgg
    59881 cagatagcct cagcacccct ctgaccaaaa gtggtgtcaa tcacagactc agaaaaatca
    59941 ataagatagc cgatgaatta taaaaccacg aatcctatgt gactcgtggt tcttttttat
    60001 aaactagtag agtgttttgg ttgtactttt tgttcagggt caatgtagtt gatagcgttg
    60061 ttgacagcag ttggagcttc tccgaggcct gtcgcaatca gatcaatttt tccgtcatag
    60121 tagcagcagt caccgatagc atagatacct gcttggctgg attcctgttt gctgttgacg
    60181 ataatcttgt gacggttgag gtcgagcccc cagtttttaa ggttaccgac agaagatttg
    60241 aaaccatagt tgacaaagag gtggtctagg tcaatggttt cagtttcatc agatttgact
    60301 tttgtgattt caagtttatc aagtgttttt ccatttccaa ggagttggct aggggcgaat
    60361 ggtgtcttga tggttacaga tgattcttgc aaggcttgaa cactgtgttc caaggcacgg
    60421 aaattatctc tgcggtgaac aagggtagtt ggtgcgattt tttcaaaagc caaagcccaa
    60481 tccacagccg agtctccccc accaagaatc gtcactttct taccagcgta ttgctgaatg
    60541 ttagaaacgt ggtagtggat attttcatag ccctcaacac tttcaagttc cagcggacgt
    60601 ggtttgaagg caccgccacc catagcgatg ataactgttt tagtcaggtg acttccttta
    60661 gaagttgtga tggcaaattc ttcttcttgt ttgtcaatct caagaaccgt ttcattgaga
    60721 tgaatagggg tatcaaatcc atttagctgt tcaatcaagc ggttagtcaa ctcttctcca
    60781 gtcaggtttg ggaagcctgg tacgtctagg atttcctttt cagggtagag aatagcaggt
    60841 tgtccaccta gctggggaag agagtcgatg atttgaacct tggcttggcg taggtgggca
    60901 taaaaggctg caaaaagccc gacaggacca ccacccacaa tggtaatatc atagagttga
    60961 gacatagttt ctcctttgtt ttttctagtc agtttatttt atcatatttt tgttcaatgt
    61021 aaaatggact aggataggga aaaaaatgcc agaaaatggt ataataggac gaaagtgttt
    61081 tgatagggag gatatcaggg atgaattttc aacaattatc caatctgcaa tattggacca
    61141 gtttgtttgc aagtccatgg acgatagcta tcaatctgat tgatattttg attgttgctt
    61201 atattttata ccattttaca aaagctattg caggaaccaa gattatgatt ttggtacgtg
    61261 gagttttggt gtttatttta gctcaaatcc ttgcaaatat gattggtttg actacgattt
    61321 cttggttaat caatcaaatt attacttatg gggttattgc ggcggttgtt atcttctctc
    61381 cagagattcg gactggtttg gaacgtttgg gaagagcgac agatttcttt tccaatgccc
    61441 ctattagtgc tgaggaacag atgattcgtg cctttgttaa gtctgttgaa tacatgagtc
    61501 ctcgtaaaat cggggccttg gttgctattc agcgtgtacg taccttgcag gagtatatgt
    61561 cgacaggaat tcccttggat gctaagattt ctgcagaact tctcattaac atttttattc
    61621 ccaacactcc cctacatgac ggtgcggtga ttatcaaaga agaacgtatc gctgtgacgt
    61681 ctgcctatct gcccttgaca aaaaacacag ggatttccaa ggaatttggg accagacacc
    61741 gggcggctat cggtttatca gaagtctcag atgccttgac ttttgtcgta tcagaggaaa
    61801 cgggaggaat ttcgataacc tataatggaa ggtttaagca caacctaaca cttgatgaat
    61861 ttgaaacaga attacgtgaa atcttacttc caaaagagga agtgggtctt agttttaaag
    61921 aacgattgct aggaggatgg aaacatgaaa aaaaatagtt tatatatcat atcctcactc
    61981 ttttttgctt gtgtcttatt tgtctatgct acggcgacga attttcaaaa cagtaccagt
    62041 gctaggcagg taaaaacgga aacctatact aatacagtaa caaatgtccc tattgacata
    62101 cgctataata gtgataagta ttttattagc ggttttgctt cagaagtatc agtggtcttg
    62161 actggtgcaa atcgcctatc gctagctagt gaaatgcaag aaagtacacg taaattcaag
    62221 gttactgctg acctaacaga tgccggtgtt ggaacgattg aagttccttt gagcattgaa
    62281 gatttaccca atgggctgac cgctgtggcg actccgcaaa aaattacagt caagattggt
    62341 aagaaggctc aaaaggataa ggtaaagatt gtaccagaga ttgaccctag tcaaattgat
    62401 agtcgggtac aaattgaaaa tgtcatggtg tcagataaag aagtgtcgat tacgagtgac
    62461 caagagacat tggatagaat tgataagatt atcgctgttt tgccaactag cgaacgtata
    62521 acaggtaatt acagtggttc agtacctttg caggcaatcg accgcaatgg tgttgtctta
    62581 ccggcagtta tcactccgtt tgatacaata atgaaggtga ctacaaaacc agtagcacca
    62641 agttcaagca catcaaattc aagtacaagc agttcatcgg agacatcttc gtcaacgaaa
    62701 gcaactagtt caaaaacgaa ttaaaaatag aaaaggattt tataacaatg ggtaaatatt
    62761 ttgggactga tggagtccgt ggagaagcta acctagaact aacaccagaa ttagccttta
    62821 aactaggacg ttttggaggc tatgttctta gtcaacatga aacggaagcg ccgaaagtct
    62881 ttgtaggacg tgacacacgt atttcagggg aaatgctgga atcggccttg gtggcaggtc
    62941 tcctttcagt agggattcac gtatacaaac ttggtgtcct tgcaacacca gcagtagctt
    63001 acttggttga aactgaagga gcaagtgccg gtgtcatgat ttctgctagc cacaacccag
    63061 cccttgataa cggaatcaag ttctttggcg gtgatggctt caaactagat gatgaaaaag
    63121 aagcagaaat tgaagccttg ctagatgctg aggaagacac tcttcctcgt ccaagtgcag
    63181 aaggcttagg aattttggta gattatccag aaggcttgcg taagtatgaa ggataccttg
    63241 tttcaactgg aactcctctt gatggaatga aggttgcctt ggatacagct aatggagcag
    63301 cttctaccag tgcccgtcaa atctttgcag accttggtgc ccaattgacg gttatcgggg
    63361 aaacaccaga cggtcttaac atcaacctta atgttggttc aacacatcca gaagcccttc
    63421 aagaagtggt caaagaaagt gggtcagcta ttggtttggc ctttgatgga gacagtgacc
    63481 gcttgattgc tgttgatgag aatggtaaca tcgtcgatgg tgacaagatt atgtacatca
    63541 tcggaaaata cctttctgaa aaaggacaat tggctcaaaa tacaattgtg acaactgtta
    63601 tgtctaacct tggtttccac aaggccttga atcgcgaagg tattaacaag gcagttactg
    63661 cagttggtga ccgctacgtt gttgaagaaa tgagaaaatc aggctacaac cttggtggtg
    63721 aacagtctgg tcacgttatc ttgatggatt acaataccac aggtgatggt caattatcag
    63781 cagttcaatt gactaaaatc atgaaggaaa ctggtaagag cttatcagag ttggcggcag
    63841 aagtaacgat ttatccacaa aaattagtta atatccgagt ggaaaacgtc atgaaggaaa
    63901 aggccatgga agtgccagct atcaaggcca tcatcgagaa gatggaagaa gaaatggcgg
    63961 ggaacggccg tatccttgtt cgtccaagtg gaacagaacc cctcttgcgt gttatggcag
    64021 aagcgcctac aacagaagaa gtagactact atgttgatac catcacagat gtagttcgtg
    64081 ctgaaattgg gattgactaa atcctagaaa actagatgaa aataaaaaat tatggtacaa
    64141 tagcttataa tattgtagcc gatggagaaa gacatgaatc ttaaacgtga acaagaattt
    64201 gttagtcagt atcattttga tgcgcgtaat tttgaatggg aaaatgaaaa tggagctcct
    64261 gaaaccaagg tggatgtgaa tttccaattg ctccaacatg accaagaaaa tcaagtgact
    64321 tccttgattg tcatcttgag ctttatgatt gtatttgata agtttgtcat tagtggaaca
    64381 atctcacaag taaaccatat cgatggccgt attgtcaatg aaccaagtga attgaaccaa
    64441 gaagaggtgg aaaccttggc tcgtccatgt ttgaacatgc tcaatcgttt gacttacgaa
    64501 gtgactgaaa ttgcattaga cttaccagga attaatttgg agttctaata tgaaattagc
    64561 tgttttcaca gattcctctg cttatctcag tgaagagacc ttgcaaagag aagacttgtt
    64621 tgtcttggat attcctgtca atattgatgg tgaggaatat gtcgaaggca tcaatctgtc
    64681 tgctgaggaa ttttaccaaa aaatggctca ggcttctgaa ttgcctaaga ccagtcaacc
    64741 aagtattgcc aagttagatg agatcttaac ttcgctcaaa gaacaaggct atacacatgc
    64801 cttggggctt ttcctatctt ctggaatttc aggtttttac caaaatatcc agtatatggt
    64861 cgatgactat gagggcttaa ccattgcttt cccagacact ttgattacaa gtgctcccct
    64921 aggcatcatg gttgaaagcg tctttaattg gcgtgatcag ggcgatgatt ttgccagcat
    64981 tcaggataag ctagccattc aaatcagccg tacgtcagcc tttatcatgg tagatgactt
    65041 ggatcatttg gtaaaaggtg gacgcctttc aaatggggct gccattttgg gcaatttgct
    65101 tagcattaag ccaatccttt attttaacga tcaaggtgtg attgaagttt acgaaaaagt
    65161 tcgtactgaa aagaaggcca ccaagcgctt aattgaaatt atcaaggaaa caacggcttc
    65221 aggtcaatac cgggtcattg tcattcatgg gaatgctcct gaaaaggctg aagaattgcg
    65281 tcagcacttg cttgattttg gcttgggttc ggatgtttca cttgctacat ttggtagtgt
    65341 cattggaacg cacctaggag caggaagtat tgctctgggt tatattccag tgatttagtt
    65401 ggcgcttcta ggctagttaa gatgccttgt atcttagaaa ttgaacacgg actacctgcc
    65461 tcttgaaaaa agatgcctgt cttgcctttc gtggaaagtc agtgccattc cctatttttc
    65521 agaggcagct aaggtccttt gtatcttgat aattgaacac gcctggaacc ctgtgtgaaa
    65581 aagatagttc ttccaagaag tagacacttc ttgattggaa ttcctatttt cactttgtgt
    65641 tcttacggac tttgtatctt agacaggagt agagatgagt attcgagtaa ttattgccgg
    65701 ttttaaggga aagatgggcc aggctgcttg tcagatggta ttgactgatc cagacttgga
    65761 cttggtagca gttttggatc cttttgagtc tgagtcagaa tggcagggta ttcctgtttt
    65821 caaggataag gctgatttag ctggttttga agcggatgtc tgggtagatt ttactactcc
    65881 agctgttgcc tacgaaaata cacgttttgc tcttgaaaat ggctttgctc cagtagttgg
    65941 aacgactggt ttcacgagtg aagaaattgc agagctaaaa gaattttctc gtgcccaaga
    66001 cttgggtggc ctgattgccc ctaactttgc cttgggtgct gtcttactca tgcaatttgc
    66061 gacgcaggct gccaaatatt tcccaaatgt ggagattatt gagctccatc atgacaagaa
    66121 aaaggatgct ccgagtggaa cagccattaa aacagctgag ttgatggcag aggttcgaga
    66181 gtccattcag caaggcgcag cagatgagga agagctgatt gctggtgctc gtggtgctga
    66241 ctttgatggt atgcacatcc actcagttcg tttgccaggt ttggtagccc atcaggaagt
    66301 catctttggc aatcagggag aagggttgac cctccgtcat gactcctatg atcgcatctc
    66361 cttcatgaca ggagtcaatt tgggaattaa agaagttgtc aagcgtcatg agcttgtcta
    66421 tggattagaa cacttattat gagattaacg caaatgcctt ctgaatttca gaaggcttta
    66481 ccagtattag aaaaaattaa agaagcaggc tttgaggctt attttgttgg gggctctgtt
    66541 cgagatgccc ttctccataa ccctatccac gatgtggata ttgcgacgtc ttcttatcca
    66601 gaagagacca agcagatttt tccgcgaaca gccgatatcg gaatcgagca tggaaccgtc
    66661 ttggtcttag atggggatga ggagtatgag gtaacaacct ttcggacaga ggatgtctat
    66721 gtggactatc gcagacccag tgcggtttcc tttgtgcgct cgctagaaga agacctcaaa
    66781 cgccgtgatt tcacagtcaa cgcctttgcc ttggatgaga caggagaaat cgttgacttg
    66841 ttccatggtt tagaagattt ggaaaagcaa gtcttgcgag cagttggagt ggctagtgag
    66901 cgtttcaacg aagatgcttt acggattatg cgtggtttcc gttttcaggc tagtcttggt
    66961 tttgcacttg aaccagaaac atttaaagct atgaagacct tgacgccgct tttggagaac
    67021 atttctgtag agcgtacctt cgtcgagttt gataaactct tgctggctcc attttggaga
    67081 aggggcttgg cttccatgat tgagagtcaa gcttatgact atctccctga tatggcatct
    67141 agccaggaca agctcaacag actgtttgat ttggagactg attttacttt tgaatcctct
    67201 gaacaagcct gggcggcttt actatgggct ttggagattg aaaatgcgca gtcatttttg
    67261 aaatcttgga agacctcacg ccagtttgcc aagcaagttc aggatttgct gattattttg
    67321 gctctgcgtg aaaatggaga attgagcaag cgagattgtt atcgctttga catagatttg
    67381 cttttacagg ctgaaaatct tcgtcaagct caaggaaaag aagtcaaccc acaagccatc
    67441 acagaaaaat accaaagctt gactatccat gacaagaaag agattcagat taatggcggt
    67501 attttgatca aggaatatgg ctatcagcca ggcccagact tgggagagat tttaacagag
    67561 attgagtttg ccattgtcga tggagaattg gagaataatc gtgaagccat ccatgcttac
    67621 ctgagggaga aaaaatgagt gattttatcg ttgaaaaact aagtaaatcc gttggtgaca
    67681 agaccgtttt tagggatatt tcctttatta tccatgactt agacagaatt ggtttaatcg
    67741 gtgtcaatgg gactggcaag accacccttt tggacgtcct ttctggtgtt tctggatttg
    67801 atggggatgt cagtcctttt tcagctaaaa atgattacca gattggttac ttgactcagg
    67861 atcctgattt tgatgataga aagacagttt tggatacggt tctatctagt gaactcaagg
    67921 aaatccagct cattcgtgag tatgaattga ttatgctcga ctatagtgag gacaagcagg
    67981 cgcgtttgga acgtgtcatg gcagagatgg actctctcca agcttgggaa atcgaaagtc
    68041 aggtcaagac cgttcttagc aaattgggca ttcaagactt atctactcct gttggggaat
    68101 tgtcaggtgg tctgagaaga cgggtacagt tggcacaagt cttacttggc aaccacgacc
    68161 tcttgctttt ggatgagccg accaaccatc tggatattgc gattattgag tggctgaccc
    68221 tctttttgaa aaattctaag aagaccgtcc tttttatcac tcacgatcgt tatttcttag
    68281 acgctttgtc aacacggatt ttcgagttgg atcgtgcagg cttgaccgag taccagggaa
    68341 attaccaggc ctatgttcgc ctaaaggcgg aacaggatga gcgcgacgcg gctcttcttc
    68401 acaaaaaaga acaactctac aaacaagaat tggcctggat gcgcagacaa ccgcaggcgc
    68461 gtgcgaccaa gcagcaggcc cgtatcaatc gattccatga tctgaaaaag gaagtttcag
    68521 gcagtagtgc tgagacagac ttgaccatga actttgaaac cagtcggatt gggaagaaag
    68581 tcatcgagtt tcaggctgtt tcctttgcct atgaaaacaa gcctattttg caagatttta
    68641 atctcttggt gcaggccaaa gaccgtatcg gaatcgttgg ggacaatggt gttggaaaat
    68701 caaccctgct caacttgatt gcaggaagtc ttgagccaac taaaggacaa gttgtgattg
    68761 gggaaactgt tcgcatcgcc tatttctctc agcaaattga gggtttggat gaaagcaaga
    68821 gagttatcaa ttacctgcag gaagtggcag aagaggtcaa gaccagtggc ggttctacga
    68881 cttcgattgc agagttgctg gagcagttcc ttttcccacg ttcgacgcat ggaaccttga
    68941 ttgagaaatt gtcagggggt gagaaaaaac gtctttatct cctcaaactg cttttggaaa
    69001 aaccaaatgt tcttctttta gacgagccaa ccaatgacct agatattgca actttgacag
    69061 tcttagagaa tttcttgcaa ggttttgcag gtcccgtttt aacagtcagt cacgaccgct
    69121 atttcttgga taaggtagcg accaagattc tcgcttttga ggatggcaag attcgtcctt
    69181 tctttggtca ttacaccgac tatcttgatg aaaaagcttt tgaaacagat atggccaatc
    69241 aagtgcaaaa ggtcgaaaag gaaaaagtgg tcaaggttcg agaagacaag aaacgcatga
    69301 cctaccaaga aaagcaggag tgggcaagta ttgaaggtga tattgaaacc ttggaaaaac
    69361 gtatcgctgc tattgaagag gaaatgcagg ctaacggctc tgactttggt aagctggcta
    69421 ctctccaaaa agaattggat gagaaaaatg aagcactcct tgaaaaatac gaacgctatg
    69481 agtatctcag tgaatttgat agttaagact attcgaaaat caaagtgcaa actatgtcgg
    69541 cttcacattg ccgactcaag tacagcctgt gaccggcttc ctaatttgca ctttgatttt
    69601 tattgagtat aaacgaatag aaaaatcccg tctcatgaaa ggatttctct attctttttt
    69661 ggtttcttga tgaaagatat tttgccaggt ttcgtggcga cgccagatac tggtatggat
    69721 gataccatct aactcatagc agataagttt ggttttttga ctgatggaag taatttgaat
    69781 gtccttgata gcagaattta actctttttt ggtcttataa gcctctttat ccatctgctc
    69841 tccatcttgg cgaatataga caaaatcatc agtcaagagt tcttctagtt ggtttccttg
    69901 gtcgatcaat tgttcacgca taagcaattt tttatagaca ttgtctaaaa tttcatcctc
    69961 agggataggt gctggttcga tatggacatc ggtatcaaag acgccaaaac gctcctccag
    70021 catagactcg acctgatccg cgatttcatg gctttcaaaa acagacaagt caggattcat
    70081 ctctagtgta atatccaggt agatgttgct accgtaggtg cgacctcttt gcgatttgac
    70141 cttgctgatt ttgggaattt ccatgatagc cttttggtag tcctcgagca ggcggtcgtc
    70201 aaagccatct gaaagactaa aggaagactc gatgaagata tcataggcag tcttcaagat
    70261 aaagaaagtg atgatgatag caaccagttt atccacaatc ggataattga aactactagc
    70321 taggatggca atggcggttc caagtgaggt aacagcgtca gaaagattgt ccttagcagc
    70381 tgccttcagc gccttggagt tggatttctt actgaggcga gtattgtaga gatagaccac
    70441 aaacataatc gctgcagaaa tgattcctag agttgcacca agaggatcaa tgaccgtttc
    70501 ttcccgactg agaatctttt gaatggtatc tcttagaaca tcgaaaccga catagaacat
    70561 gatgatagaa gtgatcaagc ttgccaaatc ttcaatcttc caatgaccaa aacggtggtc
    70621 acggtctgca ggctggcgcg ccatccgaat cccgattaag agggccacat ttccaatgat
    70681 gtccgatacg ttattaaaac catcggccac caaactggat gaatgaagga gatgaccagt
    70741 tgctaatttg gctgcagaca agatcaaata ggtcgaaata ctgataatgg ctccacgctc
    70801 agctaacttg agatttgaga tagattgctt cattcaactt cctttctagt cacatagcat
    70861 tctaccatta tactggtttt caaggaaaaa ttcaaataag gcagttttta ctctttgaaa
    70921 actccccaaa atttggtata atggtactac tcaagggagt agctggcaga aacctgtgat
    70981 agtgtcgtca ttccgaattt tatgctgaaa agtatgcttt ccggccctat cttaaacagc
    71041 gagacttgtt atgattaaca ggtctctttt tgtttgtcgt gaaaagatat gacagaaaga
    71101 gagtctgttt tgcacacaat gtcaaggagg agacacatgt caaaagaaca aaaacgccaa
    71161 gcgttttata ctcagagccc tgaagaggtc ttgcaggctg tggatgcgac cgagcaaggt
    71221 ttgtcatcaa gtgaggcgga aaagcgcctt gccgaatttg ggcacaatga actcgaagaa
    71281 ggcgagaaac gatcaatctt ggtcaaattc atcgagcaat ttaaggattt gatgattatc
    71341 atcctagttg cggcagcaat cttgtcagtc gtgacttctg gtggggaaga tatcgcagat
    71401 gccattatca tcctagctgt ggttatcatc aacgctgcct ttggtgttta ccaagaagga
    71461 aaagctgaag aagctatcga agccctcaaa tccatgtcta gtccagttgc ccgcgttctt
    71521 cgtgatggac acatggcaga gattgactct aaagaattgg taccaggcga tatcgttgcc
    71581 cttgaagcag gtgacgtggt accagcggac ctacgtttgc tagaagccaa ctctcttaaa
    71641 attgaagaag cagccttgac aggtgaatct gtaccagtcg aaaaagactt gtcagtcgat
    71701 cttgcgacag atgctggtat tggtgaccgt gtcaacatgg ccttccaaaa ctcaaacgta
    71761 acctatggtc gtgggatggg tgttgttgtc aatacaggta tgtatactga agttggtcat
    71821 attgctggta tgcttcaaga tgcggatgag actgatacac cactcaaaca aaacttgaac
    71881 aacctttcta aggtcttgac ctatgctatc ttggtcattg cccttgttac ttttgtagtg
    71941 ggtgtcttca ttcaagggaa aaatccactt ggtgagttgt tgacttctgt tgcccttgcc
    72001 gttgcagcca ttccagaagg acttcctgct atcgttacca tcgttctttc tcttggtact
    72061 caagttttgg ccaaacgaca ttccatcgtt cgtaagttgc cagcagttga aacacttggt
    72121 tcaactgaaa tcatcgcttc tgataagact ggtacgctga ctatgaacaa gatgacagtc
    72181 gaaaaagtct tctacgatgc ggttctacat gactcagctg atgatattga actaggtctt
    72241 gaaatgccac tacttcgttc agttgtcttg gccaatgata cgaaaatcga tgtggaaggt
    72301 aacttgattg gtgacccaac cgaaacagcc tttatccaat atgccttgga caagggctat
    72361 gatgtcaaag gtttcttaga gaaatatcct cgtgtagctg aattgccatt tgactctgac
    72421 cgtaagctca tgtcaacagt tcacccattg ccagatggtc gtttccttgt agcagtcaag
    72481 ggtgcgccag accaactctt aaaacgttgt cttcttcgtg ataaggctgg ggatattgct
    72541 ccgattgatg agaaggttac aaatctcatt catacaaaca attctgaaat ggctcatcaa
    72601 gccttgcgtg tccttgcagg tgcttataag attatcgata gtattccaga aaatctcact
    72661 tctgaagagc ttgaaaatga tttaattttt actggtttga ttgggatgat tgaccctgaa
    72721 cgtcctgaag ccgctgaggc tgttcgtgtg gctaaggaag cgggaatccg tccaattatg
    72781 attacaggtg accaccaaga cacagcggaa gccattgcca aacgtttggg aatcattgac
    72841 gcaaacgata cagaaggtca cgttttaact ggtgctgaac tcaatgaact gtcagatgaa
    72901 gaatttgaaa aagtcgttgg tcaatactct gtttatgccc gtgtgtctcc agaacacaag
    72961 gttcgtatcg tcaaggcttg gcaaaaacaa ggtaaagtcg ttgccatgac aggtgacggt
    73021 gtcaatgacg cgccagctct gaaaacagcc gatatcggta tcggtatggg aatcactggt
    73081 acagaggttt ctaagggggc ttctgacatg attcttgcag atgataactt tgcgactatt
    73141 atcgtcgcag tggaagaagg acgtaaggtc ttctcaaaca ttcaaaagac tattcagtac
    73201 ctactttctg ctaatactgc tgaagtatta accatcttcc tatcaacctt gtttggttgg
    73261 gatgtcttgc agccggttca tcttttgtgg atcaacttgg taacggatac cttcccagct
    73321 atcgctcttg gtgttgaacc cgcggagcct ggtgtcatga atcataaacc acgtggacgc
    73381 aaggcaagct tcttctcagg tggtgttttg agttctatca tttatcaagg tgtactccaa
    73441 gcagctcttg ttatgagtgt ttatggcctt gcgattgctt acccagttca tgtgggtgac
    73501 aatcatgcta ttcatgcaga tgccctaacg atggcctttg caacccttgg tttgattcag
    73561 ctcttccatg cctacaatgt caagtctgtc taccaatcca tcttgacagt tggcccattc
    73621 aagtctaaga cctttaactg gtccatcttg gtatccttta tccttctcat ggcaacaatc
    73681 gttgtagaac cgcttgaagg aatcttccac gtaaccaaac tagacttgtc acaatgggga
    73741 attgttatgg ctggaagctt ctcaatgatt atcatcgtcg aaatcgttaa gtttattcaa
    73801 cgcaaactcg gttttgacaa gaatgcgatt taagggaggt ttcggatgtc agcatatcaa
    73861 ttaccgaccg tatggcagga tgaagctagt aatcaaggag cttttacggg gctaaacaga
    73921 caaacagcag gtgcccgttt cgaacaaaac ttgccaaaag gagaacaagc ttttcagctt
    73981 tattcactgg gaacaccaaa tggtgtgaag gttactatct tattggaaga attactagaa
    74041 gctggtttta aggaagcggc ttacgacttg tataagattg ctatcatgga tggggatcaa
    74101 ttcggatcag actttgtgaa gctcaatcca aattccaaga ttccagcctt attggaccag
    74161 tcaggtactg aagatgtaag agtctttgag tctgctcata ttcttcttta ccttgctgag
    74221 aaatttggag cctttttacc aagtaatcct gtggaaaagg tagaagtttt gaattggcta
    74281 ttctggcaag caggtgcagc accttttcta ggtgggggat ttggacattt cttcaattat
    74341 gctcctgaaa aattggaata tcctattaac cgttttacga tggaagtgaa acgccagttg
    74401 gatttattgg ataaggaatt ggctcagaaa ccttatattg caggcaatga ctatacgatt
    74461 gcagatattg ctatctggtc ttggtatgga cagttagttc aagaaaatct ttaccaaggt
    74521 tctgcaaaat tcttggatgc ctcaagttat caaaatctag taaaatgggc agaaaaaatt
    74581 gccaatcgtc cagctgttaa gcgtggcttg gaagtaactt atacagaaat taaatagcaa
    74641 aaaagtcatc ttcggatggc tttttgctac agttgaggga aagggtttgc agttatcggc
    74701 ttttgtgcta taattgctat aatatagtaa aaagcaagag gagtgtgagg aagtggaaaa
    74761 gaaaattgtg aaggataact tatttttatc tcaagtgtct cagccggcaa gtcaggagga
    74821 cctttatctt gccagagatt tgcaggatac actcttagca aatcgtgata cctgtgttgg
    74881 tctagctgcc aatatgattg gggtgcagaa gcgcgtgatt atctttaatc ttggcttggt
    74941 tcccgtggtc atgtttaacc cagtgcttct gtcctttgaa ggatcctatg aggcagaaga
    75001 aggctgtttg tccttggtag gtgtgagatc aactaagcgt tatgaaacca taaggcttgc
    75061 ctatcgtgac agcaagtggc aggaacagac cattaccttg acaggcttcc cagctcagat
    75121 ttgccagcat gagctggatc acttggaagg acgaatcatt taggaggaaa gcaaatgaaa
    75181 cgaatagtct ttgaacttat ttttatcgca acgacctggt atatcttttt accgcccctt
    75241 aacctgacca gctgggaatt tctcttcttc ctctgtgggc atttgttagt tgtggcaatc
    75301 ttatttggct ttggcaaggg gataaacctt gtcaaaacgg ttcatgtgcg ccacggtaag
    75361 gcggaagctg ccttaaatct tgagggtttc aaaatcaatc ggttagggaa aattctgtta
    75421 gcttcgattg gaggaattct tctcttggca gctttggttt ccttggtaac ttccagcatg
    75481 tttcaggcta aaaattatgc caatgtagtc acggttacgg aaaaagactt tactgaattt
    75541 cctaggagtg acaccagtaa ggttcctatc ctagatagaa gtactgctga aaaaattgga
    75601 gaccgctact tgggttccct aaccgataag gtgtcgcaat acgtagcggc agatacctat
    75661 acccaattga caattgatgg gaaaccttat cgggtcacac cactagaata tgcagaccct
    75721 atcaaatggt ttaacaatca agccaaggga atcggtgagt atatcaaggt ggacatggta
    75781 actggaaatg cggatttggt ggacttgaag acaccaatca agtattcaga ctcggagtat
    75841 tttaaccgtg atgtcaaacg tcacctgcgc ttgaagtacc cgaccaaaat ctttaaaact
    75901 ccatcttttg aggtggacga tgagggcaat cctttctatg tagcaacggt ttaccaaaag
    75961 caatttggac ttgctgttcc tcgtcctgct tcagtcatta tcttggatgc tacaaatgga
    76021 gaaaccaagg aatacagctt atcagatgtt ccagaatggg tggacaggat ctatccagca
    76081 gaggaaacca ttgagcaaat taactacaac ggcaagtaca aggacggttt cttgaatgcc
    76141 atgatttcca agaaaaacgt gacccagact accaagggct ataattactt gtctatcggt
    76201 aatgacatct atctctacac aggtgtgacg tcggctaatg cggatgagag taatcttggt
    76261 ttcatccttg aaaatatgcg aacaggagaa atcactaagt atagcttggc ttctgcgaca
    76321 gaagaatcag cccgtgaatc agcagaaggt gctgttcagg agaaatccta caaagcaacc
    76381 ttcccaatcc tcatcaacct caatgacaag cctctctaca tcatgggctt gaaggacaat
    76441 gctggcttgg tcaaagagta cgccctggta gacgcagtcg agtaccaaaa tgttatcgtt
    76501 gctactacag tggaagagat gctcagcaag tatgccaata aaaacgacct tgaaattgac
    76561 aatgcaacga cagaaagcat caatggagta gtagcagacc tcaaatcagc tgttatcaag
    76621 ggagacactg tctacttctt taaagttgat ggcaagatat acaaggtcaa ggcttcagta
    76681 tccgatgacc ttccttacct tgaaaatggt aaaaccttcg aaggtcaagt aggaaaagac
    76741 aattatctca agacctttaa agtacaataa tactcttcga aaatctctag ctctatctgc
    76801 aacctcaaaa cagtgttttg agcagcctgc ggctagtttc ctagtttgct ttttgatttt
    76861 cattgagtat aaaaaaggtt tatttaataa atatatgtta taataaggta aattaagcct
    76921 aatggaggac aaagaaatgg gtttttattt gatggttgct tcaatgctac tcggactgct
    76981 cgctttgaag ataggttttt ctcagttcaa ggagaaaaaa gataaattct tatctatctt
    77041 aacaagttta gctggcacag cccttgttct cgtggctgtc tggttaggat ggcccaaata
    77101 aatagaaaaa cctcggtcaa accgaggttt ttcaaatgaa tttcttggtt gtggctaaaa
    77161 aatatgctac actatcaata tgaaaatttt aatcccaaca gcaaaagaaa tgaacacaga
    77221 cttgccaagt atcgaggcaa ttcctttaaa accagaaagt cagaccgtgc ttgatgcctt
    77281 ggctctctat tctgccagtc aattggagag tttctacaag gtatcagctg agaaagcggc
    77341 ggaagaattt caaaatatcc aagctttgaa aaggcaaact gctcaacact atccagcctt
    77401 gaaacttttt gatgggctta tgtaccgcaa cattaagaga gataagctga ccgaggcgga
    77461 acaagattat cttgaaaatc atgtcttcat tacctcggct ttgtacggtg ttgttccagc
    77521 cttgtcaccc atggctcctc accgtttgga ttttttgatg aaattaaaag tcgctggtaa
    77581 gactttgaag agccattgga aggcagtcta tgatgaagct ctgaagaagg aagaagtgat
    77641 tttctctctc ttgtcatcag agtttgagac tgtattttct aaggaaatca gagcaaagat
    77701 ggtgaccttc aaattcatgg aggatagagg cggtcagctg aagattcact caactatctc
    77761 caagaaagcg cgcggggcct ttctaacagc tttaatagaa aatcaagtac aaactgtggg
    77821 ggaagcacgt cgcttgaact ttgctggatt tgtttaccga gaagatttgt cacaaccaca
    77881 gggattggtt tttgtaaagg aagtatagaa atcagatatt ttgtatttgg caatatgtat
    77941 catcagctgt tttagtttat aaatgagaaa aaaagcgaac aagctagtct tctgagtgtc
    78001 agaaaagtag tcttgttcgc ttttattatg ttatactctt cgaaaatctc ttcaagccac
    78061 gtcagcgtcg ccttaccgta tatatgtcac tgacttcgtc agttctatct acaacctcaa
    78121 agcagtgctt tgagcagctt gcggctagct tcctagtttg ctttttgatt tttattgagt
    78181 gttagttact tgatagcttc gaccaagtct tgagcttgtt tttcaagtga gtttaggact
    78241 gtttcttcaa gaaccaattt tccgtctgcc caggcagagt cattaacacg tgcagcagtg
    78301 aaatcaccaa cgccttgtgt acggataaat ggcaagaggt ctttgtagat agcgaaaagt
    78361 tgatcgtgcc ctgcattggc tacagatgat actgtgacaa acttgtcttg aagggcagaa
    78421 acgccacgtg tatcagacaa gtcaagggca cgagatagcc agtcaagcaa gtttttcact
    78481 gtaccaggga tagagaagtt gtagactgga gagaaaatcc agatagcatc cgcaacgaga
    78541 actgcttcac gagcggcagc tacagctgga tgtgttggga cttccaaatc ttggctgaag
    78601 agagggaggg ctgaataatc aaggtagcta acttccgctt tactagcaag tgctttctca
    78661 gcttcgagcg ccatttggtg gttgaaagaa ccttgacgta gtgatccgac gataaataat
    78721 acttttttag acatgatgtg tctccttttt cttaaaattt cgagaactct ctcgttgtta
    78781 taaaatatat tacaacaaaa caaaagaact tgcaagaaat ttgctcagag tttatttgaa
    78841 aattgtaaaa tttcggaaac tctagtcatt tgtgactgaa aaagtaagaa ctgtttcact
    78901 ttcaaaatcg gttttgtttg aagggagaaa tagaattggt tgctcaaagc acagctttga
    78961 ggttgcagat aaagctgacg tggtttgaag agattttcga agagtattct tatattcact
    79021 tgtaataaag cttaatagat ggtaaaatag acgagtgaaa aaaagacaaa acaaagcaaa
    79081 aaataatcta ctgtggcagt atggtctagg gatgacgatt ttgtttgtgg ttatcagtgg
    79141 ttcctttctg tatatggttt ctcttagcat gaaaccctat caaacagcta aaagtgaagg
    79201 agaaaaatta gctcagcagt atgcaggatt agagcaggtc gatcaggttg atttatacaa
    79261 tggcttggaa tcttattaca gcgttcttgg tcgtaataaa cagcaagaag cgcttgctgt
    79321 cctgattggt aaagatgatc ataagattta cgtttatcag ctaaatcagg gtgtttcaca
    79381 agaaaaagca gaaacggttt ctaaggaaaa gggagctggc gaaattgaca agattatctt
    79441 tggtcgttat caagataagc caatctggga agtcaagtca ggatctgatt tttatctagt
    79501 agattttgaa acaggagcat tggtcaacaa ggagggccta tggaattatc caaccgtgtt
    79561 ttagaaatgg aagaaagtgt gactttggct gctggagccc gtgccaaagc actgaaggct
    79621 gagggtagag atattttgtc tctaaccttg ggtgagccag atttcactac tcccaaaaat
    79681 atccaagatg ccgccattgc atcgattcga gatgggcgtg cttcttttta tacagtaacc
    79741 tcaggtctgc cagagcttaa ggcggcggtc aatagctact ttgagcgctt ttacggctat
    79801 tctgtagcgt caaatcaagt gacagtcgct gcgggagcca aatattctct ctataccttc
    79861 tttatggctg tggtcaatcc aggtgatgaa gtcatcatcc caaccccata ctgggttagc
    79921 tatggagatc aggtcaagat ggcagaaggc gttcccgtct ttgtttctgc taaggaagac
    79981 aatcacttta aggtgaccgt agagcagtta gaagcagctc gcactgacaa gaccaaggtt
    80041 ttggtgctga attcgccatc taatcccaca ggtatgattt acacccgtga ggaactcttg
    80101 gcaattggaa actgggctgt agaaaatgat attctcatcc tagcagatga tatctatggc
    80161 cgcttggttt ataatggtca tgagttcaca ccgatttcta gcctatcgga agcgattcgc
    80221 aagcaaacag tggtcatcaa tggtgtgtct aaaacctatg ccatgactgg ttggcggatt
    80281 ggttatgccg ttggagaagc agacattatc gctgccatgt ccaagattgc aggtcaaaca
    80341 acttcgaatc cgtcagcagt agcccaatat gcagcagttg aggctctatc aggtgagcaa
    80401 gatactgtag aaagcatgcg tcaggccttt gaggaacgtc ttaataccat ctatcccctc
    80461 cttgcagaag tgccaggatt tgaagtggtc aaaccgcaag gggccttcta tctcttccca
    80521 aatgtcaaaa aggccatgga gatgaaaggc tacacggatg tgacagactt tacaactgtt
    80581 atcttagaag aagccgaagt ggccttggta acaggagctg gatttggagc accagaaaat
    80641 gtccgcctca gctatgcgac agacctagac acgcttaaag aagcagtcga acgcttgaaa
    80701 gcatttatgg gtagtgagaa tgattgatca ttttgagatt aaggtaaagg atttacaaat
    80761 ttcagaagga ttttatagga gttttctcgc tcctttggac tataaattgg cttttaagac
    80821 tagttctcta attagttttc tatccccgaa cagccctcat cctggtggtg atttttggct
    80881 gactcaggga acacaagatc ctgttcattt tgctttttta gcagaaaata aggaagaagt
    80941 tcaggcttgt tatgaggctg gcttagaggc aggtgggcga gacaatgggg ctcctggtta
    81001 tcgaagtgag catccgattt actatgctgc ttttatgatt gacctggatg ggaacaatat
    81061 agaggtggtt tgccataaag aataaaaaag aaaaggaaaa ataatgacaa aacgtgtaac
    81121 gattattgac gtaaaagact atgttggtca ggaagtgacg attggcgctt gggttgccaa
    81181 caaatcagga aaagggaaaa tcgctttctt acaattgcgt gatggaacag ccttctttca
    81241 aggtgtggct tttaaaccaa actttgtcga aaaatttggt gaagaagtgg gacttgagaa
    81301 gtttgatgtt atcaaacgct tgagccaaga aacgtctgtt tatgtgacag gtattgtcaa
    81361 agaggacgaa cgttctaaat ttggctatga gttggacatc acagacatcg aagtgatcgg
    81421 tgaatctcaa gactacccaa tcacaccaaa agaacacgga acagactttt tgatggataa
    81481 ccgtcacttg tggctacgct ctcgtaagca agtagctgtg ttgcaaatcc gtaacgctat
    81541 tatctatgca acttatgagt tctttgacaa gaacggtttt atgaagtttg acagcccaat
    81601 tctttcagga aatgcggcag aagattctac agaactcttt gaaactgact acttcggaac
    81661 gccagcctac ttgagccaat caggtcaact ttacctagaa gcaggggcta tggctcttgg
    81721 tcgtgtcttt gactttggtc cagttttccg tgctgaaaaa tcaaaaacac gccgtcactt
    81781 gactgagttc tggatgatgg atgctgagta ctcatacttg acacatgatg agtcgcttga
    81841 cttgcaagaa gcttatgtga aagctcttct acaaggtgtt cttgaccgcg cgcctcaagc
    81901 cttggaaacc ttggaacgtg atacagaact cttgaaacgc tacattgcag agccattcaa
    81961 acgtatcact tacgatcaag ccattgacct cttgcaagag catgaaaatg atgaagatgc
    82021 tgactacgag catcttgagc atggtgatga ctttgggtca ccacacgaaa cttggatttc
    82081 aaaccacttt ggtgtgccaa catttgtcat gaactatcca gcagccatca aggccttcta
    82141 catgaaacca gttcctggaa atccagagcg cgtgctttgt gcagacttgc ttgctccaga
    82201 aggctatgga gaaattatcg gtgggtctat gcgtgaggaa gattacgatg cccttgtcgc
    82261 taagatggat gaacttggca tggatcgtac agaatatgaa ttctaccttg accttcgtaa
    82321 atacggtaca gttccacacg gaggatttgg tatcggtatc gaacgtatgg taaccttcgc
    82381 agcaggaaca aaacatatcc gtgaagctat tccattccca cgtatgttgc accgtatcaa
    82441 accataagaa caggaaaacg cccatgtggc gttttttcga attctagtgt ttttacttgc
    82501 gtaaaaaaat tttttctagt ataatagttt attgtgagcg aacctcactt accccttgca
    82561 aagtcttggg gtcattagac caaaaggagg aacatatcaa tggctaaata cgaaattctt
    82621 tatatcattc gtccaaacat tgaagaagaa gctaaaaacg ctttggtagc acgttttgac
    82681 tctattttga ctgacaacgg tgcaactgtt gttgaatcaa aaacttggga aaaacgtcgt
    82741 cttgcatacg aaatccaaga tttccgtgaa ggactttacc acatcgttaa cgttgaagca
    82801 aatgacgatg cagctcttaa agagtttgac cgtctttcaa aaatcaacgc tgacattctt
    82861 cgtcacatga tcgtcaaaat tgacgcgtaa gaaggtaaac tatgattaac aatgttgtac
    82921 ttgtagggcg tatgacacgt gacgctgagt tgcgttatac cccatcaaat gtagcagttg
    82981 cgacttttac tcttgcagta aaccgtacat ttaagagtca aaatggtgaa cgtgaggctg
    83041 attttatcaa tgtcgttatg tggcgccaac aggctgaaaa tcttgctaac tgggctaaaa
    83101 aaggctcact tatcggggtg acaggtcgta tccagactcg tagttacgat aaccagcaag
    83161 gacaacgtgt ctacgtgaca gaggtcgtgg ctgagaattt ccaaatgttg gaaagccgta
    83221 gtgtgcgtga gggccacaca ggtggagctt actctgcacc aactgcaaac tattcagcac
    83281 ctacaaattc agtaccagac ttttcacgta atgaaaatcc atttggagca acaaacccat
    83341 tggatatttc agatgatgat ttaccattct aatggacaac taaacttata aaggagaaaa
    83401 aacatggctc aacaacgtcg tggcggattc aaacgccgta aaaaagttga ttacatcgca
    83461 gcaaacaaaa ttgaatatgt tgattacaaa gatactgagc ttcttagccg tttcgtttca
    83521 gaacgtggga aaatccttcc tcgtcgtgta acaggaactt cagctaaaaa ccaacgtaaa
    83581 gtaacaacag ctatcaaacg cgctcgcgta atggctttga tgcctttcgt aaacgaagat
    83641 taaagaaaag acccggttgg gtctcttttt catgagcttg aaaacaagaa agcgaattaa
    83701 gaccccaacg ggtctttttt cacaagcttt gaaatgagag agcgagttag gctcagtggc
    83761 tcccttttta cgagtttgaa aacaagagaa cacattaaaa gcacgatgga gctttttcat
    83821 aaatatgtct tttttctcat ccaagaaatt aaaataaaaa tgataaactt tttgaaaata
    83881 ggggatttgc ctactttatg atataatgga aagagttaga ttgaaagagg tagtgagatg
    83941 gacgcgaaat taagatacaa ggcaaagaag atcaagattg tcttttttga tattgatgat
    84001 acattgcgga attcaaagac agggtttatt ccaactacaa ttcccactgt ttttaaacag
    84061 ttgcgtgaaa aaggaatttt aacaggaatc gcctctggac gtggcatttt tggtgttgtt
    84121 ccagagattc gtgatctcaa gcctgacttt tttgtaactt tgaatggagc ttatatcgaa
    84181 gataaaaaag gtcaggtcat ttatcagcat cagattgaaa agtcagatgt tgaggagtat
    84241 atctcttggg ctaagcaaga aggaattgaa tatggcttgg ttgggagtca tgatgccaag
    84301 ttgtcgactc gcaccgatat gatgagtgaa gctatcaatc caatttatcc cgacttagat
    84361 gtagatcccg atttccatga aaaagaagat atctatcaga tgtggacttt tgaagataag
    84421 ggagatgact tgcacttgcc tgacagtctc tcagacaaac ttcgcatggt tcgttggcat
    84481 caacattcgt ctgatattgt gccgatttca ggctccaaag cgacgggggt ggaaaaggtt
    84541 gtggaacacc ttggcttgaa accagagaag gtcatggttt ttggagatgg tctcaacgac
    84601 ttggaactct ttgattatgc tggaatcagc gttgccatgg gaatttctca tgataaaatc
    84661 aaagaaaaag cagattatat tacaaaaaca ttagaagaag atggcatttt tgatgcctta
    84721 gaagtatttg gtatggtaga aaaagaattg cattttccac aagtagacat tgaaacagta
    84781 gaaggtcctc ttgcgactat taagaccaat cacggagact tacgtatcaa gcttttccct
    84841 gaacatgctc ctaaaacagt ggctaacttt gtatctcttt caaaagatgg ctactatgat
    84901 ggtgtcattt tccaccgtat tatcaaggac tttatgatcc aaggtggaga cccaactgga
    84961 actggtatgg gtggcgagtc aatctacggc gaatcttttg aggatgaatt ctcagaagag
    85021 ctttacaata tccgtggtgc tctttccatg gcaaatgctg gtccaaatac caacggcagc
    85081 cagttcttta tcgtgcaaaa ccaacaccta ccttattcga agaaagaaat tactcgtggt
    85141 ggttggccag aaccgattgc agaaatctat gccaatcaag gtgggacacc tcacctagac
    85201 cgccgtcaca cggtttttgg tcagttagct gatgaagcat cttacgctgt cttggatgcc
    85261 attgctgctg ttgagacagg agctatggac aagccagttg aagatgttgt aattgaaact
    85321 attgaaatcg aggactaaga tgaaaatcgg tgataagcta aaggggcgta ttacagggat
    85381 tcagccctac ggtgcctttg ttgagttaga gacgggtgat acggggctga ttcacatttc
    85441 agaaattcgg acaggattta ttgaaaatat tcatgaaacc ttgaaagtcg atgaagaggt
    85501 tcaagttcag gtagtggatt tagatgaatt tacggggaaa gccagtcttt ctatccgcac
    85561 tttggaggaa gaaaagtacc agtttcctag acggagacgc ttttcaagcg accgctttaa
    85621 ctacggtttt gctccctttc gacgaatgct accaatctgg actggagaag ccctgcacca
    85681 tttaaaaaag aagaagtagt ttagagtttt atattttttg taatgttaat atgaatttgc
    85741 tataatagga ccatggaaga agaacaatta ttaaaatcag gagagcgcat taaccagctc
    85801 ttttcgacag atatcaaaat cattcaaaat agagaggttt ttagctattc ggtggatagt
    85861 gttctcttat cacgatttcc acgttttcct aagaaggggt tgattgtgga tttctgtgct
    85921 gggaatggag cagtggggct ttttgctagc actcgtactc aagcacagat attgtctgtt
    85981 gagattcagg agcgtttggc ggatatggct gaacgctctg tccgtttgaa tggattagag
    86041 gagcaaatgc aggtcatctg cgatgatttg aaaaatatgc ctgctcacat tcaggggagt
    86101 aaggtggata tgattttgtg taatccaccc tatttcaagg tgaatcctta ttctaatctg
    86161 aacgagagtg aacattatct cttggctcga cacgaaatca cgactaattt ggaagaaatc
    86221 tgtcgtagtg cccagagtat tctcaagtct aatgggcgtt tggccatggt tcatcgtcct
    86281 gatcgacttc tggatatttt ggatacgtta aaacgacata atctagcccc taagcgcctg
    86341 cagtttgttt atccaaaaag agaaaaggaa gccaatatgc ttttgattga ggcgatcaag
    86401 gatggctcaa caagtggctt taaggtctta cctcctttga ttgtccacaa tgatgatggc
    86461 tcttatacgc ccgaaatcga agagatttat tatggatcat aaggcttata tgtacgttct
    86521 ggagtgtcgt gatggatcct actatatagg ctatacgact gatatgagaa gacgcctcgc
    86581 tatccacaat agtgggaagg gagccaaata tacacgagca cgcttgccag tcaaacttat
    86641 ctatgctcaa ggttttgcca gtaaggaaga agccatgtcg gctgaagctc ttctcaagcg
    86701 taagaagagg ccacagaagg aagaattttt atctgaaaat caagatagaa atttactcag
    86761 ttattttgaa gaatcgtggg gagtcctttg actcctctta cctttgtcaa aaagcgaaaa
    86821 aaatgctaca ataaagaaaa taaacaaaat gaggtattat catgtccaag attctagtat
    86881 ttggtcacca aaatccagac tcagatgcca tcggatcatc tgtagctttt gcctaccttg
    86941 caaaagaagc ttacggtttg gatacggaag ctgttgccct tggaactcca aatgaagaaa
    87001 cagcctttgt cttgaactat tttggtgtgg aagcaccacg tgttatcact tctgccaaag
    87061 cagagggggc agagcaagtt atcttgactg accacaatga attccaacaa tctgtatcag
    87121 atatcgctga agtagaagtt tacggtgttg tagaccacca ccgtgtggct aactttgaaa
    87181 ctgcaagccc actttacatg cgtttggagc cagttggatc agcgtcttca atcgtttacc
    87241 gtatgttcaa agaacatggt gtagctgtgc ctaaagagat tgcaggtttg atgctttcag
    87301 gtttgatttc agataccctt cttttgaaat caccaacaac acacccaaca gataaaatca
    87361 ttgctcctga attggctgaa ttggctggtg tgaacttgga agaatatggt ttggcaatgt
    87421 tgaaagctgg taccaacttg gctagcaaat ctgctgaaga attgattgat atcgatgcta
    87481 agacttttga actcaacgga aataatgtcc gtgttgccca agtgaacaca gttgacatcg
    87541 ctgaagtttt ggaacgccaa gcagaaattg aagctgcaat gcaagctgcc aacgaatcaa
    87601 acggctactc tgactttgtc ttgatgatta cagatatcgt caactcaaac tcagaaatct
    87661 tggctcttgg tgccaatatg gacaaggtcg aagcggcttt caacttcaaa cttgaaaaca
    87721 atcatgcctt ccttgctggt gccgtttcac gtaagaaaca agtggtacct caattgactg
    87781 aaagctttaa tgcgtaagat tttgggtgtc agctcaaaat cggaaagtct agtttgcctt
    87841 atatcgaaag gagtttcggc tccttttttc taggagtgaa gtatgttaga aaatggcgat
    87901 ttgatttttg tgagagatgg gtcagacatg ggacaggcca tccagacttc cacaggtaac
    87961 tatagccatg ttgccattta tttggatggg atgatttatc atgctagtgg acaggctggt
    88021 gttgtctgtc aagaaccggc agacttcttt gagtccaatc atttatacga cctctatgtt
    88081 tacccagaaa tggatatcca gtcggtgaag gaaagagctt gcaaacatct tggagcaccc
    88141 tacaatgctt ctttctatcc agatgcacct ggtttctact gctcccagta tatagcagaa
    88201 atcctaccta tttttgaaac tattcctatg aaatttggag atggggagca ggagattagt
    88261 gatttttgga gggagtatta catagaacta ggtctgcctg ttcctctgaa ccaagctggt
    88321 accaatccta gtcagttggc agcatcgcct ctgttacaat gtaaagaaag gaatcttcat
    88381 gattcagatt tttaatccat ctcgtttgac gagacagcca tttttggaga attgatccgc
    88441 tatctggacc agtatgagga tgtgattcta cgggaaatta aggctcaatt tccagatgtt
    88501 gcagttgata aactcatgga agagtatata aaggcaggct tgattctacg tgaaaataag
    88561 cgctattacc tcaattttcc tacgcttgaa tcacttgata gtcttgaact ggatcaagag
    88621 atttttgtca gagaagctag tccggtctat caagccttgt tggagcagag ttttgagacg
    88681 gaattgcgca atcaaatcaa tgcagctatt ttagttgaaa agacggactt tgcgcgcatt
    88741 aaaatgactc tgtccaatta tttttacaag gtcaaacagc agtatccttt gacagaaaaa
    88801 cagcaggagc tctatgacat tttaggagat gttaatcctg agtatgcact caagtatatg
    88861 acggcttttt tgttgaaatt tctcaaaaaa gaccagctta tgcagaaatg ccgtgatatc
    88921 tttgtggaca gtttggttgt cttaggctat attgtgcaaa atgaagatgg aaagtatgag
    88981 ttggctatcg attttgataa ggagagatta actttctact tagcgtgatt tcttgtttct
    89041 gagtacattg tttgactttc cttagtattc ggtataaact atatgtaacc ggtaacacat
    89101 atcggaataa actaaaggag acaatcatat gtcacttgaa aacaaattgg aacaagcaac
    89161 aggcgctgtc aaagaaggtt ttggtaaagt tactggagac agcaagacag aacttgaagg
    89221 agctgttgaa aaaacagttg ctaaggcaaa agacgttgta gaagacgcaa aaggtgctgt
    89281 agaaggtgcc gttgaaggtt tgaaaaacgt ttttactaaa gaataggaaa aaatcaaggg
    89341 tttcattttc ccttgatttt ttctattctt ataaataatt ttctgcgacg gctgtatctc
    89401 ctgggtagga ttctttcttg ccctggatga tttggtaaca atcggctccc ttacccgcaa
    89461 taataactgc atctaattcg tgatttgtga tagccattgc cgccttgatg gcttcttggc
    89521 gatccgcaat cttctcaaca ggatgattga tgtagctact aatttcatct gcaatggcca
    89581 ttgggtcttc atagttaggg tcatcagcag tcagaaagac ttgaatctca gggtgttgat
    89641 tgaggaggag gccaaagtcc ttacgacgac tttctccctt gtttcctgtt gatcccagaa
    89701 ccagagcaat ctttccggtt tgatgagttt caaccacatt gatgagtttt ttcagactat
    89761 ccccattgtg ggcatagtcg atgaagacct tggctccatt tttctgagtg aggacttcca
    89821 tacgaccagg aacgcgggtt gcagcgatgc cttttttgat gtcctcaaga cttgctccga
    89881 gacggagaca agcaagtcca gcagcaactg cattttcttg gttgaagttg ccaatgagtt
    89941 ggatatcata atctccagcg agtttacccg tagctgaaaa gctaaaggct ttggaattct
    90001 cgatttggtt atcaaattgg ctaccataga aatcatggtc ttgatcttca acctgttctt
    90061 tcaagactga gaagtggtcc atgtcactgt taatgatgac tgctcggcta ttttccatca
    90121 agagacgctt gtggtagaaa tagtcttcaa agctagggtg ttcaatcggg ccgatatggt
    90181 ctgggctgat attgaggaaa actcccacat caaaggttag accatagaca cgtctgacca
    90241 gataggattg actggagact tccatgatga ggtgggtacg gtcattttgc acagcctgat
    90301 tcatcatgtc aaagaggtca atactctcag gggttgtcaa cgctgactta aagaaagtct
    90361 cgccatcaag agttgtgttc atggtcgaca acatagcagg tcgatgccct tgagataaga
    90421 tgttataggc gaaataggct gctgttgtct tacccttagt accagtaaag gcaaggagtt
    90481 tgagtttttc ctgtggatta ccatagaact ccatggcaat caaactcatg gctttcttta
    90541 tatcgttcac aatgatgaca gggataccga cttcgtagtc cttttcagct acataccaag
    90601 ctaagccttg tgttatagca gaaagaaggt attctttttt aaaggcagcg ccttttgcaa
    90661 aaaaaagagt gtcttctgtt acttttcggc tatcgtagct gatgctatca aaaacaactt
    90721 tgctgtagtt gtagtggtaa tgaccttggt caataatttc gcgaaaaagg ccatctttct
    90781 ttaaaatatc taatacagtt tcaattttta tcatactttc tattgtaaac cgaaagtcgc
    90841 aaatttacaa gtaacaagga aaagtttata atggaagata aggagttttt cctagttatc
    90901 aaaattgaat gaggaatcta tgtcgcacga aaacaatcac cagcaggccc agatgttacg
    90961 ggggactgct tggctaacgg ctagtaactt tatcagtcgc ctactcgggg ctgtttacat
    91021 tatcccttgg tacatctgga tgggggctta tgcagctaag gcaaatggtc tctttaccat
    91081 gggttacaat atctatgctt ggttcttgtt ggtttcaaca gcggggattc cagttgcggt
    91141 ggccaagcaa gttgccaagt ataataccat gcgagaagaa gaggatagct ttgccctgat
    91201 tcggagcttc ttaggcttta tgacaggact aggcctggtt tttgctttag tcttgtatgt
    91261 ctttgctcct tggctagcag acttgtctgg cgtgggcaaa gacttgatcc caatcatgca
    91321 aagcttggct tggggagtct tgattttccc gtctatgagt gttatccgag gatttttcca
    91381 agggatgaat aacctcaaac cttatgccat gagccaaatt gctgagcagg tcattcgtgt
    91441 tatctggatg ctcctagcaa cctttatcat tatgaagctc ggttcaggag attatctagc
    91501 agccgttacc caatcaacct ttgctgcctt tgtcggtatg gtagccagtt ttgcagtctt
    91561 gatttatttc cttgcccaag aaggttcact caaaagagtc tttgaaacag gagataagat
    91621 taacagtaag cgtctcttgg ttgataccat taaggaagcc attcctttta tcctgacagg
    91681 gtctgccatc cagctcttcc agattttgga tcagctgacc tttatcaata gtatgagctg
    91741 gtttaccaac tacagcaatg aggacttggt tgtcatgttt tcttatttct cagccaatcc
    91801 taataaaatc acgatgattt tgatttctgt aggggtttcg attgggagtg ttggtttgcc
    91861 acttttgacg gaaaactatg tcaaggggga cttgaaagca gcttctcgtc tggttcagga
    91921 cagtctcacc ctactcttta tgttcttgct accagcaacg gttggagtgg ttatggtagg
    91981 agaacctcta tatacagtct tctatggtaa gccagatagt ttggctctgg gcttatttgt
    92041 ctttgcagtt ttgcagtcta ttattttagg cttgtacatg gtcttgtctc caatgcttca
    92101 ggccatgttc cgcaaccgca aggccgttct ctattttatc tatggttcta ttgccaagct
    92161 agtcttgcaa ctacctacca tcgccctctt ccacagttat ggtcctttga tttcaacaac
    92221 cattgctctc atcattccta acgtcttgat gtatcgggat atttgtaaag taactggtgt
    92281 caagcgcaag gtgattttga agcgaaccat tttaatcagt ttgctgaccc tagtcatgtt
    92341 tctgttaata ggaaccatcc agtggctgtt aggatttttc ttccaaccaa gtggacgttt
    92401 gtggagcttc ttttatgtag ctcttgtcgg tgccatgggg ggtggacttt atatggttat
    92461 gagtctgcgt acctatttat tagataaggt aataggaaaa gcccaagcag atcgcctgcg
    92521 agcaaaattt aagctttcgt aaggtagcat ttataaataa aaggaataag ttgaacaatc
    92581 ttttaggaga acttgaaact tattcctttt ttagcgtcaa aatagctaaa attatctttc
    92641 aaaaatagaa aatgaggttt ttttgatttt ttggaaaaaa caattgacta aacaaaccaa
    92701 gagttgtata ataaaacaaa tatttaaagc aggaggttct ggaaatgaaa aagtctaaga
    92761 gcaaatatct aaccttggca ggtcttgtcc tgggtacagg agttttattg agcgcgtgtg
    92821 gaaattctag cactgcatca aaaacctata actatgttta ttcaagtgat ccatctagtt
    92881 tgaactatct agcagaaaac cgcgcagcaa catccgatat tgttgcaaat ttggtagacg
    92941 ggttattaga aaatgaccaa tatgggaata ttattccatc attagcagag gattggactg
    93001 tttctcagga cggtttgacc tatacctaca aacttcgtaa ggatgccaag tggtttactt
    93061 ctgagggaga agaatatgcg cctgtaactg cccaggattt tgtgacaggt ttgcaatatg
    93121 cagctgataa aaaatcagaa gctttgtatc tagtgcagga ctctgttgct ggtttggatg
    93181 actatatcac tggtaaaaca agcgactttt caactgtcgg tgtcaaggca cttgatgacc
    93241 aaacggttca atatactttg gttaaaccag aactttactg gaattcaaaa acacttgcaa
    93301 cgatactttt tcctgttaat gcagatttcc tgaaatcaaa aggggatgat tttgggaagg
    93361 cggatccatc tagtattttg tacaatggac ctttcttgat gaaagcactt gtctcaaaat
    93421 ctgctattga atataagaaa aaccctaatt actgggatgc taagaatgtc tttgtagacg
    93481 atgtgaaatt gacctactat gatggtagcg accaagaatc actggaacgt aattttacag
    93541 ctggtgctta tactacggct cgtctttttc ctaacagctc cagctatgaa gggattaaag
    93601 aaaaatacaa aaacaatatc atctatagta tgcaaaattc aacttcatat ttctttaatt
    93661 ttaacctaga taggaagtct tacaattata cttctaaaac aagtgacatt gaaaagaaat
    93721 cgacccagga agcagttctc aataaaaact tccgtcaggc tatcaatttt gcttttgaca
    93781 gaacatctta tggggctcag tctgaaggga aagaaggtgc aacaaagatt ttgcgtaacc
    93841 tagtggttcc tccaaacttt gtcagtatca agggaaaaga ctttggtgaa gttgtagcct
    93901 ctaagatggt caactatggt aaggaatggc aaggtatcaa ctttgcggat ggtcaagacc
    93961 cttactacaa tcctgagaaa gccaaggcta agtttgcgga agctaagaaa gaactcgaag
    94021 caaagggtgt tcaattccca atccacttgg ataagactgt ggaagtaaca gataaagtag
    94081 gcatacaagg agttagttct atcaaacaat caattgaatc tgttttaggt tctgataatg
    94141 tagtgattga cattcagcaa ttaacatcag atgagtttga cagttcaggc tactttgctc
    94201 aaacagctgc tcagaaagac tatgatttat atcatggcgg ttggggacct gattatcaag
    94261 acccgtcaac ctatctcgat atttttaata ctaatagtgg aggagttcta caaaatcttg
    94321 gactagagcc tggagaagcc aatgacaagg ctaaggcagt tggactggat gtctatactc
    94381 aaatgttgga agaagctaat aaagagcaag atccagccaa acgttatgag aaatatgctg
    94441 atattcaagc ttggttgatt gatagttctt tagttcttcc aagtgtttcg cgtgggggaa
    94501 caccatcatt gagaagaacc gtaccatttg ctgctgccta tggtttaacc ggtacaaaag
    94561 gggttgaatc atataaatac ctcaaagtac aagataagat tgtcacaaca gacgaatatg
    94621 caaaagccag agaaaaatgg ttgaaagaaa aagaagaatc caataaaaaa gcccaagaag
    94681 aattggcaaa acatgtcaaa taaaatctaa ttgtagataa gtttgtgtaa gaagtctttg
    94741 tgcttaaaaa cgcataatat caggtatata aaatcctaga tgttatgcgt tttgttatag
    94801 gaagatttat tagattttat cctcaaattg agttattccc gactttatcc gttgaaatat
    94861 tataaggagt atttatgaaa ttaaaattat taagagtaga tactaaggtg attatgggga
    94921 gtttcttact tgttctgtct agtctacttg ctttgttgct tccccttatc ttaaagggtt
    94981 taatagatgg gagttctatt gaaaatatag gttccaaagt atttcaatcg tttttgattt
    95041 ttattggtca agccttgttt tcttctattg gttactatct gtttagtcaa tcgggtgaaa
    95101 aaaagatagc aaaaatcagg aaaaaagtga tagaggggtt gatttatgca gagaaatcct
    95161 tctttgataa gagccaaagt ggggagttga cttctgccat tgtcaatgac atgagtgtca
    95221 ttcgtgagtt tttaattacg actttcccaa atattattct gagtttagtt atggtacttg
    95281 gttccattgt agtcttattt agtcttgatt ggaatctttc tctactttta ttcatcactc
    95341 ttccttgtat gatgtttatt atcttgcccc tttccaatat cagtgaaaag tatagtcgtc
    95401 gtttacagga ggaaatttaa cagggcaatt gactgaaaag attcaagaac atgaattaat
    95461 taagactaac caagcagaga aaagtgtaca ggatgttttg gataattgta ttgaaagggt
    95521 acaaaacaat tcactgaaat cagatagggt tacttctttt gagacctcgt ttgctctctt
    95581 atttatcttt gcgactatag ttgtgatgct aacctatggg ggttatcggg tcagcgcagg
    95641 atatatatct gtgggaacct tggtttcgtt tttgatttac ctctttcaat tacttaatcc
    95701 tattagtaat atagctaatt ttgtaactgt ttattctagg agcaagggat cttcagttgc
    95761 actggataac ttgcttgcag ttcctaaaga aaaatttgag ggaggaaaat cggtatcagg
    95821 acaagggttg aattttaacc atgtctattt tggttatgat gaaaatcgac ctgtcttaaa
    95881 ggatattact tgttcaattt tcaaggggca aaaattgctt ttgttggacc atctggatca
    95941 ggaaaatcaa cgattgtgcg tttgttagag cggttttata aaccgctttc aggagatatt
    96001 ctaatggagc aatcaagtat atatgatttt aacttaaaag aatggagaag taaaatcgct
    96061 tgggtttcac aaaataatgc agtcttatct ggcagtattc gtgacaatct ttgtctcggt
    96121 ttgaatcgct tagtaactga tgatgaattg atgaaagtgc tagacttagt atcactaggt
    96181 gatgagattc gctccatgaa agagggacta gatactgaag ttggtgaacg cggacgattc
    96241 ttgtcagggg ggcaaagcca aagacttcaa atagctagaa cctacttaaa agatgctgaa
    96301 attcttatat ttgatgaagc tactgctaat cttgatgcgg attctgagta tgcgattatc
    96361 agtagcctct attctgtatt aaaggagaag acggttgtga ttatagcgca tagtttgtca
    96421 acggtaaaag atgtggattg tattttcttc ttagaggagg ggaaaatcac tggctcagga
    96481 actcataagg aactactgga aaatcatgag cgttatgctc gttttgtgca ggagcaaatg
    96541 atagagtgaa gtgtcttttg agattcacca ttttatagtc tattaaaggg agcaggaaaa
    96601 actccctttt tatatagttt gaaactataa ctagctcttg aaaagaagaa aatgagttga
    96661 tgaaaataag tggtacaata gttactatag atttggaggt attgtatgag caaggaatta
    96721 cacattaaca caattttggc ccaggcgggt attaagtcag atgaagcgac aggtgcattg
    96781 gtgacaccgc ttcatttttc aacgacctat cagcatccag agtttggtcg atctactggg
    96841 tttgactata cgcgcactaa aaatccaact cgtagtaagg ctgaggaagt cttggcggct
    96901 attgagtcag cagactatgc cttagcgact agctcaggga tgtcagctat tgtactggcc
    96961 tttagcggct ttccagtagg aagtaaggtc ttggcagtgc gtgatcttta cggcggttct
    97021 tttcgctggt ttaaccaagt ggagcaggaa ggtcgtttcc attttaccta tgccaataca
    97081 gaagaagagt tgattgccga gttagaaaag gatgtggatg ttctctatat cgaaacccca
    97141 accaatccct tgatgttgga atttgatatc gaaaaactag caaaattggc tcatgctaag
    97201 ggtgccaaag tggtggtgga caataccttc tatagcccta tctaccaacg tccgattgaa
    97261 gatggagcag atatcgttct ccattcagca accaagtatc tagcaggcca caatgatgtc
    97321 ttggctggag tggttgtgac caatagttta gaactatacg agaagctttt ttacaatctt
    97381 aatacaacag gggcagtctt gtctccattt gacagctatc agttgcttcg tggtctcaag
    97441 accttgtctc ttcgtatgga gcgctcaaca gctaacgccc aagaagtggt tgcctttttg
    97501 aaggattctc cagcagttaa ggaagttctc tacactggtc gtggaggcat gatttccttt
    97561 aaagtagccg atgaaacacg cattcctcat attttgaaca gtctcaaggt cttctctttt
    97621 gcggaaagtt tgggtggagt ggaaagtctt attacttatc caacgactca aactcatgct
    97681 gatattccag cagaggtacg ccattcttat ggtttgacag atgacctctt gcgtttgtct
    97741 attgggattg aggatgctag agatttgatt gcagatttgc gccaagccat agaaggataa
    97801 gacaaagatg ggaaaatatg attttacaag cctgcccaac cgtttagggc accataccta
    97861 taaatggaaa gaaacagaaa cggatagtga agttctacca gcttggatag cggatatgga
    97921 ctttgtggtc ttgcctgaaa tccgccaagc cgtgcaaact tacgcagacc aactggttta
    97981 tggttatacc tatgccagtg aagacttaat taaggaagtt caaaagtggg aagctacaca
    98041 atacggttac aactttgaca aagaggctct tgtctttatc gagggtgtgg taccagccat
    98101 ctcaacagct attcaaacct ttacaaaaga aggcgaggcg gttttaatta acacgcctgt
    98161 ctacccaccc tttgctcgca gtgtcaagtt gaataatcgt agattgatta ctaattcctt
    98221 agtggaaaag gatggtctgt ttgagattga ctttgaccaa cttgaaaagg atttggtgga
    98281 agaggaggtt aaactctata ttctttgcaa ccctcacaat cctggtggac gtgtttggga
    98341 aaaagaagtg ttggagaaga ttggccaact ctgccaaaaa cacggtgttt tgttagtttc
    98401 ggatgagatt caccaagatt tgaccctctt tggtcacaaa caccagtctt tcaataccat
    98461 caatcctgcc ttcaaaaatt ttgctatcgt cttgagcagt gccactaaaa catttaatat
    98521 tgctggaaca aaaaattcct atgcagtcat tgaaaatcct aagttgagac tagctttcca
    98581 gaaacgcctg ttggccaata atcagcatga aatttcaggc ttgggttatt tggcgacaga
    98641 agctgcctat cgatacggta aagattggct agaggaactc aagcaagtct ttgaagacca
    98701 catcaattat gtggtggatc tatttggaaa agagactaaa atcaaggtca tgaaaccgca
    98761 aggtacctac ttgatttggc ttgacttttc agcttatgac ctgactgatg aaacattgca
    98821 agagttgttg agaaatgaag ccaaggttat cctcaaccgt ggtttggatt ttggagagga
    98881 aggaagtctc catgcccgca tcaatatagc tatgcccaaa tctctgttgc aagaagtctg
    98941 tcagcggatt gtggctactt ttgccaaacg ttaaaaatcc agccttctag gagaaaagtc
    99001 ttcctagaag gctattttca taggcgaaaa tatggtataa taaaaagata aggtaaaggt
    99061 gaaaatatgg ctaaattgat tccggggaaa attcgtatcg aaggtgttgc cctttatgaa
    99121 actggtaagg ttgatatcat caaggaaaag aacaatcggc tctacgctcg cgttgcagaa
    99181 gaagaactgc gctatagttt agaggatgat ttggtttttt gtgcctgtga tttttttcaa
    99241 aagaggggtt actgtgtgca tttggcagcg ctagagcatt ttctgaaaaa tgatgagcgt
    99301 ggtcaggaaa tcttgtggag tctggaagaa ggtcatgaag aaaaagaggc tgttgaaacc
    99361 aaggtgacct tgggtggcaa gtttttggat cgaattttat ctccaaaatc agaatgcgcc
    99421 tatgagttat cagctgtggg tcaggtggaa gcaggaacca atcatatctt gtggactctg
    99481 agaatcggcc aaatcaatag ccaaaaatac tatgtcattc gggatattcc acttttttta
    99541 aggattgtcg agcagagaaa gtcgtatatg attggcaaga tttatgagga gtctctttct
    99601 tgggaagcct ttgatgatgc cagccaagaa cttttgactt tcttgcacgg actaacggag
    99661 gaagggctgg tttcagacct ctttttccaa aaccaaggtc ggcacctctt tttccctctg
    99721 accttttttg agcagggtgt gaatttactg atggccttgc cccattttca atttgaccat
    99781 caagtggata gctaccaaac cctgcttttt caggatatgc atgctgatgc caatctattt
    99841 gcctttacag taacagaata ctcggactat tttgaaatgg aaatcagtga gagtcctagg
    99901 gtcaatgtct tttatcaggg agcagtactt ttccataaag gtcaggttta ttttttaaca
    99961 gaccagcaga tgcgtcttct caaggaaatt aaagcgttac ctgtgggtca acaaggaaag
   100021 aaatacctgc aatttgatag cagtgatcga gacaaattgg cctcctgtct gactcttttt
   100081 atccagatgg gcaccgtttc agctccagaa cgtctacaaa tcaaaacttt tgcgccctct
   100141 ttttactttg acagggaaga agataatcgg attcgtttag aaattcagtt tgattatgga
   100201 aatagacagg tatctagccg acaagagcta gaagaattac cattttcaag tgacgcggac
   100261 ttagaagaaa gaattttcca agtctgtttg gctgcgggct ttgaagccga ttttcaatct
   100321 tggcgtcagg ccttaaaagc ggagtctgtc tatcattttt ttcatgatat cattccagtc
   100381 tttgaaaaac tcgggcatgt tgacctatca gataaactag aagagcttta tagcctagcg
   100441 agtcctcagg tgcagattgc ctccaaggga ggtctcttgg aaatccagtt tgattttcaa
   100501 gatattgccc aggaggaaat cgaccaagcc atgcaggcct tggttgccaa tcaggatttt
   100561 tatattgatt cgtctaatca agtttacttt ttcgatgaag aaaccaagaa aattcgccaa
   100621 aatctacagg aactggggca atttgaatta aaagatggga ccttgcaggc tcggaaatcc
   100681 ttggcctaca gtttagctca tctctttgaa gggcgagacc gtgtttcttt ttcacaagaa
   100741 ttccagaatc tggcccagga tttgacacat ccagaggact ttcctcgaca ggcaactcag
   100801 gttcaggctg acttgcgaga ttatcaggaa aagggaattg gctggttgca gatgctccat
   100861 cattatggtt ttggtgggat tttggctgat gatatgggac ttgggaaaac ccttcagacc
   100921 attgcttttt tgactagtca agtgacaaaa gaaagtcggg ttttgattct cgctccgtca
   100981 ggtttgattt acaactgggc agatgagttt cagaaatttg ccccacagtt ggatgtggct
   101041 gttgttcatg gtttgaaagc aagtcgtgag gagattcttg ccgagagcca tcaaatctat
   101101 gtgactagct atgccacctt ccgtcaggac agtgagtttt atcaggggat ggcctttgac
   101161 ttccttttct tagatgaggc tcaggtcatg aaaaatgccc agaccaagat tgcccagacc
   101221 ttgagacaat ttgtggtgcc gtcggtcttt gccttgtcag gaactccgat tgaaaaccat
   101281 ctgggtgagt tgtggtctat tttccaaatc gtcatgcccg gacttttgcc aagcaagaaa
   101341 gaatttatga aattgccagc agagcgagtg gctcagttta tcaagccttt cgtgatgcga
   101401 cgcaagaaag aagaagttct gactgaattg ccagacttga ttgaggtggt ttataaaaat
   101461 gaactggaag accagcaaaa ggctatttac cttgcccagt tacaacagat gcgagaccat
   101521 ctggctcaag tgtcagaaca ggaatttcag cgaagtcgtg tggaaatttt atctggtttg
   101581 atgcgcttgc gtcaaatatg tgacactcct gccctgttta tggaagatta tcagggagcc
   101641 agcggcaaac tagatagtct ccgagatcta ctggtacagg tggcagacgg cggacaccgt
   101701 gtcttgattt tctctcagtt caagggaatg ttggaaaaaa ttgaacaaga actgccagac
   101761 ttgggcctga catcctttaa aattacgggt ttaaccccag ccaaggaaag acaagacatg
   101821 accaaggcct ttaaccaagg agaaagagat gcctttctga tttccctcaa ggctggtggg
   101881 gtcggtctga acctgacagg tgcagataca gtgatcttgg ttgacctttg gtggaatcct
   101941 gcggtggaag cgcaagccat tggccgtgcc catcggatgg gtcaggaaga aacggttgag
   102001 gtctatcgct tggtgaccaa gggaaccatt gaagaaaaaa ttcaggaact ccaagaacaa
   102061 aagaaacatc tggtgtcaca agtattggat ggcacagagt cacgtggtag tctgacccta
   102121 gcagaaatta gagaaatttt gggaatttct gaagccagca cttgaaaaat caagacaata
   102181 cgctataatg aagtgttgaa aggaaataga aaatgaaaca agaacgattt ccattggtgt
   102241 cagatgacga ggtcatgttg actgaaatgc cagtcatgaa tctctatgat gagtctgatc
   102301 tgatcagtaa tatcaagggt gagtatcgag ataaaaatta tttagaatgg gctcctatta
   102361 ctgaagaaaa accagtaaaa ccgattgaaa agcaagtcga aaaacctaaa aaggctcctt
   102421 taggggttaa aaaagaagga aagagctatg cggaggtggc gcgtgaagaa gcgcgtgcgg
   102481 acttgaaaaa gaaacgctct gctaactacc taactcagga tttcagcctt gcgagacgtc
   102541 attctcagcc cagtctagtt agacagggca atcaaccgac aactcctttc caaaaggaaa
   102601 atcctggtga atttgtcaaa tatagccaaa aattgaccca gtctcattat atcttggcgg
   102661 aagaagttca ttctatccct accaagaatg aagaagtgtc agcacctgct ccaaagaaaa
   102721 acaattatga ttttctaaag aagagccaaa tctacaataa aaaaagtaaa caaacagaac
   102781 aagaacgtcg ggttgcccaa gagttgaatc tgaccagaat gacagaatag gggagaaaac
   102841 atgtcaaaga catatcattt tatcggaatt aagggatcag ggatgagtgc cttggccttg
   102901 atgttgcacc agatggggca caaggttcag ggatcagatg ttgaaaagta ctactttacc
   102961 caacgcggtc ttgagcaggc aggaattacc attcttcctt ttgatgaaaa gaatctagac
   103021 ggtgatatgg aaattatcgc tggaaatgcc tttcgtccag ataacaacgt cgaaattgcc
   103081 tatgcggacc aaaatggtat cagctacaaa cgttaccatg agtttctagg tagctttatg
   103141 cgtgactttg ttagcatggg agtagcagga gcacatggaa aaacttcaac gacaggtatg
   103201 ttgtctcatg tcttgtctca cattacagat accagcttct tgattggaga tgggacaggt
   103261 cgtggttcgg ctaatgccaa atattttgtc tttgaatctg acgaatatga gcgtcacttc
   103321 atgccttacc acccagaata ctctattatc accaacattg actttgacca tccagattat
   103381 ttcacaagtc tcgaggatgt tttcaatgcc tttaacgact atgccaaaca aattaccaag
   103441 ggtctttttg tctatggtga agatgctgaa ttgcgtaaga ttacgtctga tgcaccaatt
   103501 tattattatg gttttgaagc tgaaggcaat gactttgtag ctagtgatct tcttcgttca
   103561 acaactggtt caaccttcac cgttcatttc cgtggacaaa acttggggca attccacatt
   103621 ccaacctttg gtcgtcacaa tatcatgaat gcgacagccg ttattggtct tctttacaca
   103681 gcaggatttg atttgaactt ggtgcgtgag cacttgaaaa catttgccgg tgttaaacgt
   103741 cgtttcactg agaaaattgt caatgataca gtgattattg atgactttgc ccatcatcca
   103801 acagaaatta ttgcgacctt ggatgcggct cgtcagaaat acccaagcaa ggaaattgta
   103861 gcagtctttc aaccgcatac ctttacaaga accattgccc tgttggacga ctttgcccat
   103921 gctttaaacc aagcagatgc tgtttatcta gcgcaaattt atggctcggc tcgtgaagta
   103981 gatcatggtg acgttaaggt agaagaccta gccaataaaa tcaacaaaaa acaccaagtg
   104041 attactgttg aaaatgtttc tccactccta gaccatgaca atgctgttta cgtctttatg
   104101 ggagcaggag acatccaaac ctatgaatac tcatttgagc gtctcttgtc taacttgaca
   104161 agcaatgttc aataggatgt tcccatggaa attccaatta agatcattca ggcaagcaag
   104221 tttgatttgc ctgagatagg ggcacttcag acctcgtctt ttccagctga aaagcagcaa
   104281 ctttcccata ttttagaaga aagtatccgt aagtgtgcgg atacctttct tctagctagg
   104341 gatgaaaatc aacttttagg ctatatttta tcaagtcccc agtcagacaa tccgcaatgt
   104401 ctaaaagtac attctttagt catcgagtct gaccatcaga gacagggcct gggaacactt
   104461 cttcttgcag ccttgaaaga ggtggcagtt gagctggatt acaaagggat tcgtttggag
   104521 agtcctgatg agctgctttc ctattttgaa atgaacggtt ttgttgatga agaagaaatt
   104581 ttgctctatg caactagcca gggctatagt atgatttggt ttaatccctt ttatctggag
   104641 gaacaatgaa aatcagacaa gcaagattag aagatttgga tcggattgtt gaacttgaat
   104701 ttgaaaattt ttcggtcgaa gaagccattc ctccttctgt ctttgaagca catttgagag
   104761 aaattcagac ctcttttctg gttgctgaaa aagaaggaag aatcatgggt tatatcgaag
   104821 gaccagttgg cctgcaccgc catctgcaag accagtcttt tacagaagaa ataaaagact
   104881 atagtcatga gcctggtggt tatatctctg tgacctgtct gtctattgcc aaggaggcac
   104941 agggactcgg actgggtcag aaattgctga cagccttgaa agaagttgct cttgaagatg
   105001 agagagatgg cattaatcta acctgtcatg actatctcat cgcctattat gaaaaacatg
   105061 gatttgtcaa cgaaggccag tcccagtcaa cctttgcagg ggaaacatgg tatgatatgg
   105121 tctgggaaat gaaaaaataa gttaggaaaa gtatcataga ttgctttttt tggactttta
   105181 agatataata ttatggatta tcaacaagga ggaaaaactt ttgagtgaaa agtcaagaga
   105241 agaagagaaa ttaagcttta aagagcagat tctgagagat ttagaaaaag taaaaggcta
   105301 tgatgaagtt ctgaaagaag atgaggcagt agttcgcact cctgcaaatg aaccttcagc
   105361 tgaagaactc atggctgatt ccttgtcaac ggtagaggag attatgagaa aagctcctac
   105421 cgtgcctact cacccaagtc aaggtgtacc agcttctcca gcagatgaga ttcaaagaga
   105481 aactcctggt gttccaagtc atccaagtca agatgtacct tcttctccag cggaagaaag
   105541 tggatcaaga ccaggtccag gtcctgttag acctaagaaa cttgaaagag aatacaatga
   105601 aaccccaaca agggtagctg tttcctatac gacggcagag aaaaaagcag aacaagcagg
   105661 tccagaaaca cctacgcctg ctacagaaac agtggatatc atcagagata catcacgtcg
   105721 tagccgtaga gaaggagcaa aacccgttaa gcctaagaaa gagaagaagt cacatgtgaa
   105781 agcttttgtg atttcattcc ttgtattcct tgccttgctc tcagcaggtg gttactttgg
   105841 ttaccagtac gtgctagatt ccttattacc tatcgatgct aattctaaga aatatgtgac
   105901 ggttggaatt ccagaaggtt caaacgttca agaaatcggt acgacgcttg aaaaagctgg
   105961 tttggtaaag catggtctga tttttagttt ttatgccaag tataaaaatt ataccgactt
   106021 gaaagcaggt tactacaatt tgcaaaagag tatgagtaca gaagacttac tcaaagagtt
   106081 gcaaaaaggt ggaacagatg aaccgcaaga acctgtactt gcgactttga caattccaga
   106141 aggttatacc ttggatcaga ttgctcaaac tgtgggtcaa ttgcaaggtg acttcaaaga
   106201 gtctttgaca gcggaggctt tcttggctaa agttcaagat gagacgttta tcagtcaagc
   106261 agtagcgaaa tatcctactt tactggaaag tttgcctgta aaagacagcg gtgcgcgtta
   106321 tcgtttggaa ggataccttt tcccagctac atactctatc aaggaaagca caactattga
   106381 gagcttgatt gatgagatgt tagctgctat ggataagaac ctatctcctt actatagtac
   106441 tatcaaatct aaaaacttga ctgtcaatga gttgttgacc attgcttcct tggtcgaaaa
   106501 agaaggtgcc aagacagaag atcgtaagct cattgcaggt gtattctaca atcgtttgaa
   106561 tcgtgatatg ccacttcaaa gtaatattgc aatcttgtat gcccaaggaa aactggggca
   106621 aaatatcagt ctagctgagg atgttgcgat tgataccaac attgattcac cttataatgt
   106681 ttataaaaat gtaggtctca tgcctggtcc agtcgatagt ccaagtctgg atgcgattga
   106741 gtcaagcatc aatcaaacta agagcgataa cctctacttt gtagcagatg tcacagaagg
   106801 caaggtctac tatgctaaca atcaagaaga ccacgaccgc aatgtcgctg aacatgtcaa
   106861 cagcaaatta aactaaacaa actaaaatta tgtgatactt cacataattt tctttagaaa
   106921 atattatcag aagaaagttg agaaaaatgg cagaaaaaac atatcctatg acccttgagg
   106981 aaaaggaaaa acttgaaaaa gaattagaag aattgaaatt ggttcgtcga ccagaagtgg
   107041 tagaacgcat taagattgcc cgttcatacg gtgacctttc agaaaacagt gagtacgaag
   107101 cagctaagga tgaacaagcc tttgtcgaag gacaaatctc tagcttagaa acaaaaatcc
   107161 gctatgctga aatcgtcaat agcgacgcag ttgcccagga cgaagtagcg attggtaaaa
   107221 cagtcaccat ccaagaaatt ggtgaggacg aagaagaagt ttatattatc gtaggttcag
   107281 ctggtgcgga tgcctttgca ggtaaggttt caaatgaaag cccaattggg caggccttga
   107341 ttggcaagaa aacaggtgat acagcaacca ttgaaacgcc tgttggtagc tatgatgtaa
   107401 aaatcttgaa ggttgaaaaa acagcctaaa aacagaaaaa ggagtgggga ggcgatgtgc
   107461 ttcactcact cctttttcca ttttgctact cttcgaaaat ctcttcaaac cacgtcagcg
   107521 tcgccttgcc gtatgtatgg ttactgactt cgtcagtttc atctacaacc tcaaaacagt
   107581 gttttgagca acctgcggct agtttcctag tttgctcttt gatttttatt gagtattaga
   107641 tgtatagtgc attgaagtta aaatagtaca ctttgatttc taagacattg ttagaaatta
   107701 gattaaattc cttaatcgat ttgcgcatgt tttatttcat ttcactatac tttagattgg
   107761 aaaataagga atcacataaa tcgcaagaaa tagggaatct gattcgtgtt tataaccgtt
   107821 caaaaagaga agaggctgaa agtgagccac ttaatcttta tgtcgaagat gaaaagggca
   107881 atctcctggc aggtttgata gcagagactt ttggaaattg gctagaaatc gagtatttgt
   107941 ttgtaaaaga ggaactgcga gggcaaggaa tcggttcaaa actattgcaa caggcagaaa
   108001 gtgaagctaa gaatcgaaat tgttgttttg cctttgtcaa tacttaccag ttccaggcac
   108061 cggactttta tcagaaacat ggctacaagg aagttttttc tttgcaagac tatctctaca
   108121 ttaggcaaag atattattac caaaagaatc tgtaaggtga gtatggaggt ataatacaaa
   108181 gagaggctcg cacataagta tggaggtgga ctgatgtata tgagtaacta ttgataaata
   108241 ccagttataa cctagaaatt gtgtctttct agacgatatt gaggacaata taatcgtagc
   108301 cccaaaatta ggaatcaaga cttatcaggt taagaaaaga agtgatgttg ttgatatttt
   108361 aaacaattta tttaaactaa aaactctcta tctattaatc cgagattccc ctctcggatt
   108421 ttgtgttgct ttcattaaat ttacaggtaa gacttgctag cttcagtaaa tatacacaag
   108481 tttttcagaa tagagtgaaa tccaaaagaa aatggaaata atgaaagctc ttgtaggcat
   108541 aagaaaacac ctctgtgtta tacttgttgt tcaaccacaa acacaagaaa ggcacagaaa
   108601 tgcaagacaa ttatactaca aaaggtaaac atttgacaat cgatagccgt cgcttaatcg
   108661 aaagatggaa aaaagaagga aaatcaaata gagaaattgc ctctctactt ggaaaagctc
   108721 ctcaaactat ccacactaaa atcaagcgta ggacagtccg aaaatgtctt ggaaaagggc
   108781 gcttcaaaga ggtttattct gccgactatg ctcaacagtc ttatgaaaat aatcgcaagc
   108841 actcggtcaa gagatcaagc gtgaccaagg aactaaagga aaagattctc cactatcata
   108901 accaaaaatt tttgcctgaa atgatggtta tggctaaagg ggttaacgtg ggaatttcaa
   108961 ccatttacta ttggattcat catggaaaat tggggtaaag caagcaggat ctgctttacc
   109021 ctagaaaagg aaaagcgctt aagaaacagg ccagcaccaa ctttaaacct gctggtcaat
   109081 ccatcgaaca gcggcctgaa gctatcaatt ttcgcttgga gaatgggcat tatgagattg
   109141 atacggttct acttacgaga gcgaaaaact actgcttgct tgtcttgacg gatcgaaaaa
   109201 gtagacatca gatcatccga ttgattccaa ataaaagtgc tgaggtggtc aatcaggctc
   109261 taaaactcat cttaaaacaa cacaagattc tttccatcac ggcagataat ggaacggaat
   109321 tcaatcgctt gtctaatgta ttttctgagg agcacattta ttatgcacat ccctatgcct
   109381 cttgggaaag gggaactaat gagaatcaca acaggctcat tcgtagatgg ttacctaagg
   109441 gaaccaagaa aatgactccc aaagaagtcg cattcatcga aaagtggatt aacaactatc
   109501 ctaaaaaatg cttggactac aagtcaccca gagaagactt ctggatggct aacttgaact
   109561 tgaaatttag caaaatggaa ataattttta ttaaacgctt ccaatagcgg ttaaaagttg
   109621 acaaatttaa attttaggac tacactaagt aagtaaattt tattttaaag gagaatttgt
   109681 tatgaattta acatttttag gcttatgtat tgcctgtatg ggcgtatctg tcggtgaagg
   109741 tttattgatg aatggactgt ttaaatcagt agcacgccaa ccagatatgc tttctgagtt
   109801 tcgtagtttg atgtttttag gtgttgcctt tattgaagga actttctttg taactcttgt
   109861 cttctcattt attatcaaat aaatacatgg aacgagaaga aaagggagga ttttagatgg
   109921 aagaaagtat taatccaatc atctctattg gtcctgttat cttcaatctg actatgttag
   109981 ccatgacttt gttgattgtg ggagttattt ttgtctttat ttattgggca agccgcaata
   110041 tgaccttgaa acccaaagga aagcaaaatg tacttgagta tgtctatgac tttgttattg
   110101 gatttacaga acctaacatt ggttcgcgct acatgaaaga ttactcactc tttttccttt
   110161 gtttattcct tttcatggtg attgccaata accttggctt aatgacaaag cttcaaacga
   110221 tcgatgggac taactggtgg agttcgccaa ccgctaattt acagtatgac ttaaccttat
   110281 cttttcttgt cattttgttg acacatatag aaagcgttcg tcgtcgtgga tttaaaaaaa
   110341 gtataaaatc ttttatgagt cctgtttttg tcataccgat gaatatcttg gaagaattta
   110401 caaacttctt atctttggct ttgcggattt ttgggaatat ctttgcagga gaggtcatga
   110461 cgagtttgtt acttcttctt tcccaccaaa ctatttattg gtatccagta gcctttggag
   110521 ctaatttggc ttggactgca ttttctgtct ttatttcctg catccaagct tatgttttta
   110581 ctcttttgac atctgtgtat ttagggaata agattaatat tgaagaggaa tagaaaggag
   110641 taactgatgc acgtaacagt aggtgaatta attggtaatt ttattttaat cactggctct
   110701 tttattcttt tgctagtctt gattaaaaaa tttgcatggt ctaatattac aggcattttc
   110761 gaagaaagag ctgaaaaaat tgcttcagat attgacagag ctgaagaagc ccgtcaaaaa
   110821 gcagaagtat tggctcaaaa acgcgaagat gaattggctg gtagccgtaa agaagctaag
   110881 acaatcattg aaaatgcaaa ggaaacagct gagcaaagta aggctaatat cttagcagat
   110941 gctaaactag aagcaggaca cttaaaagaa aaagccaatc aagaaattgc tcaaaataaa
   111001 gtagaagctt tacagagtgt taagggtgag gtcgcagatt tgaccatcag cttagctggt
   111061 aaaatcatct cacaaaacct tgacagtcat gcccataaag cactcattga tcagtatatc
   111121 gatcagctag gagaagctta atggacaaga aaacagtaaa ggtaattgaa aaatacagca
   111181 tgccttttgt ccaattggta cttgaaaaag gagaagaaga ccgtatcttt tcagacttga
   111241 ctcaaatcaa gcaagttgtt gaaaaaacag gtctgccttc ttttttaaaa caagtggcag
   111301 tagacgagtc ggataaggaa aaaacaattg cttttttcca agattctgtg tcgcctttat
   111361 tacaaaactt tatccaggtt ctggcctaca atcacagagc aaatcttttt tatgatgtgc
   111421 ttgtagattg cttgaaccga cttgaaaaag aaacaaatcg atttgaagtg acgattacgt
   111481 ctgctcatcc tctaactgat gaacagaaga ctcgtttgct ccctttgatt gagaaaaaaa
   111541 tgtctctgaa agtaaggagt gtaaaagaac aaatcgatga aagtctcatt ggtggttttg
   111601 tcatttttgc caatcacaag acaattgatg tgagtattaa acaacaactt aaagttgtta
   111661 aagaaaattt gaaatagaaa gtggtgttct tttggcaatt aacgcacaag aaatcagcgc
   111721 tttaattaag caacaaattg aaaatttcaa acccaatttt gatgtgactg aaacaggtgt
   111781 tgtaacctat atcggggacg gtatcgcgcg tgctcatggc cttgaaaatg tcatgagtgg
   111841 agagttgttg aattttgaaa acggctctta tggtatggct caaaacttgg agtcaacaga
   111901 cgttggtatt atcatcctag gtgactttac agatatccgt gaaggcgata caatccgccg
   111961 tacagggaaa atcatggaag tccctgtagg tgaaagtctg attggtcgtg ttgtggatcc
   112021 gcttggtcgt ccagttgacg gtcttggaga aatccacact gataaaactc gtccagtaga
   112081 agcaccagct cctggtgtta tgcaacgtaa gtctgtttca gaaccattgc aaactggttt
   112141 gaaagctatt gacgcccttg taccgattgg tcgtggtcaa cgtgagttga ttatcggtga
   112201 ccgtcagaca gggaaaacaa ccattgcgat tgatacaatc ttgaaccaaa aagatcaaga
   112261 tatgatctgt atctacgtcg cgattggaca aaaagaatca acagttcgta cgcaagtaga
   112321 aacacttcgt cagtacggtg ccttggacta cacaatcgtt gtgacagcct ctgcttcaca
   112381 accatctcca ttgctcttcc tagctcctta tactggggtt gctatggcgg aagaatttat
   112441 gtatcaaggt aagcatgttt tgattgtata cgatgatcta tcaaaacaag cggtagctta
   112501 tcgtgaacta tcgctcttgc ttcgtcgtcc tccaggtcgt gaagccttcc caggggatgt
   112561 tttctatctc cacagccgtt tgcttgagcg ctcagctaaa gtttctgatg aacttggtgg
   112621 tggatcaatt acagccctac catttatcga gacacaagca ggagatatct cagcctatat
   112681 cgcaaccaac gtgatttcta tcactgatgg acaaatcttc cttggcgatg gcctcttcaa
   112741 tgcaggtatt cgtccagcca tcgatgcggg ttcatctgta tctcgtgtag gtggttctgc
   112801 acaaatcaaa gccatgaaga aggttgctgg tacacttcgt atcgaccttg cttcataccg
   112861 tgagttggaa gcctttacta agtttggttc tgacttggac gcagcaacac aggctaagtt
   112921 gaaccgtgga cgtcgtaccg ttgaggtctt gaaacaacct gttcacaaac cattacctgt
   112981 tgagaaacaa gtaaccattc tttatgcttt gacacatggt ttcttggata ctgttccagt
   113041 agatgatatt gttcgtttcg aggaagagtt ccatgccttc tttgatgctc aacatccaga
   113101 gattttggaa accattcgtg atacaaaaga cttgccagaa gaagcagtct tggatgctgc
   113161 gattacagag tttctcaatc aatctagctt ccaataagaa tagaggtgtc agatggcagt
   113221 gtctctaaat gatattaaaa caaaaatcgc ctcaacaaaa aatacgagtc aaatcactaa
   113281 tgccatgcaa atggtatcgg ctgctaagtt aggtcgctct gaagaagctg ctcgcaactt
   113341 ccaagtttac gctcagaaag tgcgtaaact tttgacagat atccttcatg gtaatggagc
   113401 tggtgcttca actaatccga tgttgattag ccgttctgtg aagaagacag gctatatcgt
   113461 tatcacttca gaccgcggtt tggttggagg ttataattcc tctattttga aagctgttat
   113521 ggagttgaaa gaagaatacc acccagacgg taaaggtttt gaaatgatct gtatcggtgg
   113581 gatgggagct gatttcttta aggctcgcgg tattcaacca ctttatgaat tacgtggctt
   113641 ggcagaccaa cctagctttg atcaagttcg taagattatt tcaaaaactg ttgaaatgta
   113701 ccaaaatgaa ctctttgatg agctttatgt ttgctacaac caccatgtca atacgctaac
   113761 cagtcaaatg cgtgtggaac aaatgcttcc gattgttgac ttggatccaa atgaagcgga
   113821 tgaagagtac agcttgactt ttgaattgga aaccagccga gaagaaattc tggagcagtt
   113881 gttgcctcag tttgcagaaa gtatgattta cggtgccatt atcgatgcca agacagctga
   113941 gaatgctgcg ggtatgacag ccatgcaaac agcgacagat aatgctaaga aagtcatcaa
   114001 tgatttgaca attcagtata accgtgccag acaggcggcg attacacaag aaattacaga
   114061 aatcgtagca ggtgctagtg ccttagaata ggccctagtc cagctcgtat gaaaatgaac
   114121 ttaggaccta gttgagctag gaaccgacag tatcttatat agaataggag aaggagatga
   114181 gttcaggtaa aattgctcag gttatcggtc ccgttgtaga cgttttgttt gcagcagggg
   114241 aaaaacttcc tgagattaac aatgcacttg tcgtctacaa aaatgacgaa agaaaaacaa
   114301 aaatcgtcct tgaagtagcc ttggagttag gagatggtat ggttcgtact atcgccatgg
   114361 aatcaacaga tgggttgact cgtggaatgg aagtattgga cacaggtcgt ccaatctctg
   114421 taccagtagg taaagaaact ttgggacgtg tcttcaacgt tttgggagat accattgatt
   114481 tggaagctcc ttttacagaa gacgcagagc gtcagccaat tcataaaaaa gctccaactt
   114541 ttgatgagtt gtctacctct tctgaaatcc ttgaaacagg gatcaaggtt attgaccttc
   114601 ttgcccctta ccttaaaggt ggtaaagttg gacttttcgg tggtgccgga gttggtaaaa
   114661 ccgtcttaat ccaagaattg attcacaaca ttgcccaaga gcacggtggt atttcagtat
   114721 ttgctggtgt tggggaacgt actcgtgagg ggaatgacct ttactgggaa atgaaagaat
   114781 caggcgttat cgagaaaaca gccatggtct ttggtcagat gaatgagcca ccaggagcac
   114841 gtatgcgtgt tgcccttact ggtttgacaa tcgctgaata cttccgtgat gtggaaggcc
   114901 aagacgtgct tctctttatc gataatatct tccgtttcac tcaggctggt tcagaagtat
   114961 ctgccctttt gggtcgtatg ccatcagccg ttggttacca accaacactt gctacggaaa
   115021 tgggtcaatt gcaagaacgt atcacatcaa ccaagaaggg ttctgtaacc tctatccagg
   115081 ctatctatgt gccagcggat gactatactg acccagcgcc agcaacagcc ttcgctcact
   115141 tggattcaac aacaaacttg gaacgtaagt tggtacaatt gggtatctac ccagccgttg
   115201 acccacttgc ttcaagctca cgtgccttgg cacctgaaat cgttggagaa gagcactatg
   115261 cagttgctgc tgaagtaaaa cgtgtccttc aacgttacca tgaattgcaa gatatcattg
   115321 ctatccttgg tatggatgag ctttctgatg aagaaaagac cttggttgct cgcgcccgtc
   115381 gtatccagtt cttcttgtca caaaacttca acgttgcgga acaatttact ggtcagccag
   115441 gttcttatgt tccagttgct gaaactgtac gtggctttaa ggaaatcctt gatggtaaat
   115501 acgaccactt gccagaagat gccttccgtg gtgtaggttc tatcgaagat gtgattgcaa
   115561 aagctgaaaa aatgggattt taagaggtga tctatggctc agttaactgt ccagatcgtg
   115621 acaccagatg gtctcgtcta tgatcaccat gccagctatg tatcggttcg aactctggat
   115681 ggtgagatgg ggatcttgcc acgacatgaa aatatgattg cagttttagc agttgatgaa
   115741 gtaaaggtaa aacgtatcga tgataaagat cacgtgaact ggattgcagt aaacggtggt
   115801 gttattgaaa ttgccaatga tatgatcaca atcgtcgctg actctgcaga acgtgctcgt
   115861 gatatcgata tcagtcgtgc agaacgtgcc aaacttcgtg cagaacgtgc aattgaagaa
   115921 gcacaagaca aacatttgat tgaccaagaa cgtcgtgcta agattgcttt gcaacgtgct
   115981 attaaccgta ttaatgtcgg aaatagacta taagaaaaaa tgaacttgaa aataccaagt
   116041 tcatttttta tggtgtttta aggagcaaaa cggatgcaga ctgcttcggg aacatggaag
   116101 tcgttggaga gttctgctag acgaccattg tcacaattac gtttaaagac agttgcattg
   116161 tcagagtctt gatggacaac aatgagaaat ttttggtcgg gtgtcaaatc aaaatcacgt
   116221 ggagtctgac catgcgttgg aacgatttct aataactcta agctaccgtc cgcaaggatg
   116281 gtatatactg cgatagaatc atggccacgg ttagaagcgt agaggtattt accgtcttta
   116341 gagagatgaa tagcagcggt tccattaaag ccttcgtaag cttccggtaa agttgaaatg
   116401 acctgcatac gttcaaattc gccaacgcca tcgtagatta aaacttcgat agtactattg
   116461 agttcacaaa tgagataagc gattttatag tggttatgga aaatgatatg gcgtgagcct
   116521 gctcctggct tgctgtgata ggtatagagc ttagataatt ttccttcttg atcgaggtca
   116581 taggtgatga cttggtcagt tcccaagtcg caggtcacta gatagtggtc aggtgttaaa
   116641 tctgtatagt gaacatgggg ggaagcttga ttttcatgtg gaccttggcc actgtgttga
   116701 tccatatcac taagtagaag actaccatct tcctggcgtt tataaacaag gacttgtccc
   116761 ttgtgatagt tggctgcgta aaccaaatca cgcttttcat cgacagcaac ataacagtgg
   116821 ggagctcctt cttcaacaac atgatttaac acagtcccgt cagtttgata ggctgcaatt
   116881 ccccccttat cgtcttggct accaacagtg tataaatgtt ggtgctggtc aaaggcaagg
   116941 taggttggac ttggctcagc tgcaaaaagt tctagatttg aaagctgacc agtttctgta
   117001 tcaaagtctg ccttgtaaat cccttgagaa gtacgacgtg tataagttcc aaaataaaca
   117061 gtttctttca ttactatacc tctgtgtaaa gataagacta ttatatcaca aaaacaagca
   117121 aattaaagac attcaattag atgtaagcac tttaaaaaag agttattttg tttcaaaaat
   117181 ggtataatga gagaacaata gaaaggaagt atttatggag caaaaagaga aacattttag
   117241 cctatcttgg tttttcaagt ggtttttaga taacaaggca attacggtat ttttagtaac
   117301 cttattattg ggattgaatc tttttatttt aagtaagatt agttttctat tttcacctgt
   117361 tttagacttt ttagcagttg tgatgttgcc agtcattttg tctggtttgt tatattattt
   117421 gttgaatcct attgttgatt ggatggagaa gcataaggtt aatcgtgtta tagctatcac
   117481 tattgtcttt gttatcatcg ctctctttat catttggggc ttggcagtcg ccattccaaa
   117541 tctgcaacgt caggttttga cctttgcaag aaacgttcct gtttacttag aagatataga
   117601 taggattgtt aatggattgg tagcccagca tctgccagat gatttcagac ctcaattaga
   117661 gcaagttttg accaattttt ctagccaggc tacagttttg gcaagtaagg tttcatctca
   117721 ggcagtcaac tgggtgagtg cctttattag cggggcttct caagtgattg ttgccttgat
   117781 tatcgttcct ttcatgctct tttatctctt gcgtgatggg aaaggcttgc gtaactattt
   117841 gacccaattc attccaagaa aattgaagga acctgttgga caagttctat cagatgtgaa
   117901 tcaacagttg tccaactatg ttcgagggca agtgacagtg gctattattg tagcagtaat
   117961 gtttatcatc ttcttcaaga ttattggtct acgctatgcg gttacgctgg gggttactgc
   118021 tggtatttta aatctggtcc cttatcttgg tagctttcta gccatgcttc ctgccctagt
   118081 attgggtttg attgctggtc cagtcatgct tttgaaagta gtgattgtct ttattgtaga
   118141 acaaactatt gaaggccgtt ttgtctctcc attgattttg ggaagtcaat taaacatcca
   118201 ccctattaat gttctctttg ttttgttaac ttcaggatct atgtttggta tctggggagt
   118261 tttacttggt attccagttt atgcctctgc taaggttgtc atttcagcca ttttcgaatg
   118321 gtataaggta gtcagtggtc tatatgaatt agagggtgag gaagtcaaga gtgaacaata
   118381 gtcaacagat gttacaggct ttggaggagc aagatttaac taaggctgag cattatttcg
   118441 ccaaagcttt agaaaatgat tcaagtgatc ttctgtatga gttggcaact tatcttgaag
   118501 ggattggttt ctatcctcag gccaaggaaa tttacctgaa aattgtagag gattttccag
   118561 aggttcatct taatctagct gcaattgcta gcgaggatgg tcaaatagaa gaagccttta
   118621 cctatcttga ggaaatccaa gctgacagtg actggtatgt ctcgtctttg gttttgaagg
   118681 cagaccttta ccagctggaa ggtttgacag atgtggcacg tgagaaatta ttggaggcct
   118741 tgacctactc agaggattct ctcttgatat tgggtttggc agagttggat agtgagttgg
   118801 aaaattacca agcggctatt caagcctatg cccagttaga taatcgctcg atttatgagc
   118861 aaacgggcat ttccacctat caacgaattg gctttgccta tgctcagtta gggaaatttg
   118921 aaacggctac tgagttttta gaaaaagccc tggagttaga atacgatgac ttaacagctt
   118981 ttgagttggc cagtctttat tttgatcaag aagaatatca aaaagccacc ctctacttta
   119041 agcagcttga taccatttct cctgactttg aaggctatga gtatgggtac agtcaggctt
   119101 tacataagga acatcaagtt caagaagccc tgcgtatcgc taagcaagga ttagagaaaa
   119161 atccctttga aactcgcctc ttgctagctg cttcacaatt ttcttatgaa ttgcatgatg
   119221 ctagtggtgc agaaaattat ctccttactg caaaagaaga cgctgaggat acagaagaaa
   119281 tcttgcttcg tttagccact atttatctgg agcaggagcg ttatgaggat attctagact
   119341 tgcagagtga ggagccagaa aatcttttga ccaagtggat gattgctcgt tcttatcaag
   119401 aaatggacga tttggatact gcttatgagc attatcaaga gttgacagga gatttgaagg
   119461 acaatccaga atttctggaa cactatatct atctcttgcg tgaattggga cattttgaag
   119521 aagcaaaagt ccatgctcac acttacttaa aactggttcc agatgatgtg caaatgcaag
   119581 aactgtttga gagattgtaa gaatgtttaa acatatagaa ctgtagttta tctcttttga
   119641 tagctgcggt ttttatttgt atatggtaga atctttttac aaaaatactt ggtaatcttg
   119701 tttattcatg ctataatagg aacaattact tttaggaggt gcagtatgtc ttatttattt
   119761 gagatattac cgagtttact gaatggtgcg agcacgactg tacaggtctt tgcactggtc
   119821 ttgctatttt cgattccgtt gggcgttttg attgcctttg ccttgcaagt ccattggaag
   119881 cccctccatt atctgattaa catttacatc tgggttatgc gaggaacccc cttactcttg
   119941 caactgattt ttatctatta tgtgctccca agtattggga ttcgtttaga ccgccttcct
   120001 gcagctatta ttgcctttgt tctcaactat gcagcttact ttgcagaaat tttccgtggg
   120061 ggaattgaca ctattccaag aggacagtat gaggctgcca aggtcttgaa gtttagccct
   120121 tttgacagag tgcgctatat tatcttgccc caagtgacca agatcgttct tcctagtgtc
   120181 tttaatgaag ttatgagttt ggtcaaggat acttctttgg tctatgctct cggaatttca
   120241 gaccttatct tggctagtcg aacagctgct aaccgcgatg ctagtctggt tcctatgttc
   120301 ttggcaggag ccatttactt gattttaatt gggattgtga caattatttc caaaaaagtt
   120361 gagaagaagt atagttatta tagatagggg gcagctatgt tagaattacg aaatatcaat
   120421 aaagtctttg gagacaaaca aatcctgtct gatttcagtc taagtattcc tgaaaagcaa
   120481 atcctggcta tcgttggacc ttctggtgga ggtaagacaa ctcttttacg tatgcttgca
   120541 ggtcttgaaa ccattgattc agggcaaatc ttttataatg gacaaccttt agagctagat
   120601 gaattgcaga agcgcaacct actgggattt gtcttccaag attttcaact atttcctcat
   120661 ctatcagttc tggaaaattt gactttatcg cctgtgaaga ccatgggaat gaagcaggaa
   120721 gaggctgaga agaaggcgag tgaactcttg gaacagttag gactaggagg acacgcagag
   120781 gcctatcctt tctcactatc tggtgggcaa aagcagcggg tggctttggc gcgtgctatg
   120841 atgattgacc cagaaatcat tggctacgat gaaccaactt ctgccctgga tccagaatta
   120901 cgtttggaag tggagaagct aatcttgcaa aatagggaac ttgggatgac ccagattgtg
   120961 gttacccatg atttgcagtt tgctgaaaat atcgcagatg tattattgaa agtagaacct
   121021 aaataggagg aaaaatggat gaaaaaatgg atgcttgtat tagtcagtct gatgactgct
   121081 ttgttcttag tagcttgtgg gaaaaattct agcgaaacta gtggagataa ttggtcaaag
   121141 taccagtcta acaagtctat tactattgga tttgatagta cttttgttcc aatgggattt
   121201 gctcagaaag atggttctta tgcaggattt gatattgatt tagctacagc tgtttttgaa
   121261 aaatacggaa tcacggtaaa ttggcaaccg attgattggg atttgaaaga agctgaattg
   121321 acaaaaggaa cgattgatct gatttggaat ggctattccg ctacagacga acgccgtgaa
   121381 aaggtggctt tcagtaactc atatatgaag aatgagcagg tattggttac gaagaaatca
   121441 tctggtatca cgactgcaaa ggatatgact ggaaagacat taggagctca agctggttca
   121501 tctggttatg cggactttga agcaaatcca gaacttttga agaatattgt cgctaataag
   121561 gaagcgaatc aataccaaac ctttaatgaa gccttgattg atttgaaaaa cgatcgaatt
   121621 gatggtctat tgattgaccg tgtctatgca aactattatt tagaagcaga aggtgtttta
   121681 aacgattata atgtctttac agttggacta gaaacagaag cttttgcggt tggagcccgt
   121741 aaggaagata caaccttggt taagaagata aatgaagctt tttctagtct ttacaaggac
   121801 ggcaagttcc aagaaatcag ccaaaaatgg tttggagaag atgtagcaac caaagaagta
   121861 aaagaaggac agtaagataa aatagtggct gaaactgcgt tttgattagc aaaacgtagt
   121921 ttttttgtaa tctaggaaaa cgataatagc gattgaatct ggataatt
//
LOCUS       DAVLXF010000002        57496 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLXF010000002 DAVLXF010000000
VERSION     DAVLXF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.002"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(124..1104)
                     /locus_tag="VYO12_000129"
     CDS             complement(124..1104)
                     /locus_tag="VYO12_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1586803.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTPPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1420..3435)
                     /gene="recG"
                     /locus_tag="VYO12_000130"
     CDS             complement(1420..3435)
                     /gene="recG"
                     /locus_tag="VYO12_000130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1586804.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3454..4557)
                     /locus_tag="VYO12_000131"
     CDS             complement(3454..4557)
                     /locus_tag="VYO12_000131"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1586805.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4547..4915)
                     /locus_tag="VYO12_000132"
     CDS             complement(4547..4915)
                     /locus_tag="VYO12_000132"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1586806.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(4954..5985)
                     /locus_tag="VYO12_000133"
     CDS             complement(4954..5985)
                     /locus_tag="VYO12_000133"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1586807.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMELENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEVFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(5987..7018)
                     /locus_tag="VYO12_000134"
     CDS             complement(5987..7018)
                     /locus_tag="VYO12_000134"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1586808.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7099..9612)
                     /gene="secA"
                     /locus_tag="VYO12_000135"
     CDS             complement(7099..9612)
                     /gene="secA"
                     /locus_tag="VYO12_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1586809.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9758..10045)
                     /locus_tag="VYO12_000136"
     CDS             complement(9758..10045)
                     /locus_tag="VYO12_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586810.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLTFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10056..10706)
                     /locus_tag="VYO12_000137"
     CDS             complement(10056..10706)
                     /locus_tag="VYO12_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1586811.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGNNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISKDISDLCTDVLAVENGHIQYVKDIQS"
     gene            complement(10710..11351)
                     /locus_tag="VYO12_000138"
     CDS             complement(10710..11351)
                     /locus_tag="VYO12_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586812.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEVNLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            complement(11344..12135)
                     /locus_tag="VYO12_000139"
     CDS             complement(11344..12135)
                     /locus_tag="VYO12_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586813.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAILTHFFGTFSPLGWNSLFSDGSSLQR
                     LLDGEITSYLFFTCVLLIGIFINAVYFLQIVDYLGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12320..12655)
                     /locus_tag="VYO12_000140"
     CDS             complement(12320..12655)
                     /locus_tag="VYO12_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586814.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNVADIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(12795..13130)
                     /locus_tag="VYO12_000141"
     CDS             complement(12795..13130)
                     /locus_tag="VYO12_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586815.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13221..14531)
                     /gene="der"
                     /locus_tag="VYO12_000142"
     CDS             complement(13221..14531)
                     /gene="der"
                     /locus_tag="VYO12_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1586816.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14545..15258)
                     /locus_tag="VYO12_000143"
     CDS             complement(14545..15258)
                     /locus_tag="VYO12_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1586817.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15255..16151)
                     /gene="dnaI"
                     /locus_tag="VYO12_000144"
     CDS             complement(15255..16151)
                     /gene="dnaI"
                     /locus_tag="VYO12_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1586818.1"
                     /translation="MESVGDVLKRQPSRFHYQDLVQKIMKDPDVAAFVQQESLNQDEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVMNHGYADVSYEETPELIAAEKEAA
                     IKKRLNLINFPSSLKNVSFLDVYRDDVQRLTVLKRMIEFVNDYPNNLKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16152..17321)
                     /locus_tag="VYO12_000145"
     CDS             complement(16152..17321)
                     /locus_tag="VYO12_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1586819.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLVLHAPLSSQD
                     FLEHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     EQKAKTQKQNQTAPAKTNIPKWSNPEYKNTSSAEELEEMERQTLELLAKLDNGGD"
     gene            complement(17322..17795)
                     /gene="nrdR"
                     /locus_tag="VYO12_000146"
     CDS             complement(17322..17795)
                     /gene="nrdR"
                     /locus_tag="VYO12_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1586820.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            17935..18300
                     /locus_tag="VYO12_000147"
     CDS             17935..18300
                     /locus_tag="VYO12_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1586821.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18305..19000
                     /locus_tag="VYO12_000148"
     CDS             18305..19000
                     /locus_tag="VYO12_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1586822.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19017..19784
                     /locus_tag="VYO12_000149"
     CDS             19017..19784
                     /locus_tag="VYO12_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586823.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20137..21336)
                     /locus_tag="VYO12_000150"
     CDS             complement(20137..21336)
                     /locus_tag="VYO12_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1586824.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21333..22226)
                     /locus_tag="VYO12_000151"
     CDS             complement(21333..22226)
                     /locus_tag="VYO12_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1586825.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22342..22422
                     /locus_tag="VYO12_000152"
                     /pseudo
     CDS             22342..22422
                     /locus_tag="VYO12_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            22907..23120
                     /locus_tag="VYO12_000153"
                     /pseudo
     CDS             22907..23120
                     /locus_tag="VYO12_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            23535..24152
                     /locus_tag="VYO12_000154"
     CDS             23535..24152
                     /locus_tag="VYO12_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1586826.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(24472..25359)
                     /locus_tag="VYO12_000155"
     CDS             complement(24472..25359)
                     /locus_tag="VYO12_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1586827.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(25508..27391)
                     /locus_tag="VYO12_000156"
     CDS             complement(25508..27391)
                     /locus_tag="VYO12_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1586828.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            27768..29222
                     /locus_tag="VYO12_000157"
     CDS             27768..29222
                     /locus_tag="VYO12_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1586829.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            29203..30168
                     /locus_tag="VYO12_000158"
     CDS             29203..30168
                     /locus_tag="VYO12_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1586830.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(30203..31477)
                     /locus_tag="VYO12_000159"
     CDS             complement(30203..31477)
                     /locus_tag="VYO12_000159"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1586831.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(31477..32649)
                     /locus_tag="VYO12_000160"
     CDS             complement(31477..32649)
                     /locus_tag="VYO12_000160"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1586832.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(32780..33028)
                     /locus_tag="VYO12_000161"
     CDS             complement(32780..33028)
                     /locus_tag="VYO12_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586833.1"
                     /translation="MVSSWGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(33096..33719)
                     /locus_tag="VYO12_000162"
                     /pseudo
     CDS             complement(33096..33719)
                     /locus_tag="VYO12_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(33781..34230)
                     /locus_tag="VYO12_000163"
     CDS             complement(33781..34230)
                     /locus_tag="VYO12_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1586834.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(34232..34990)
                     /locus_tag="VYO12_000164"
     CDS             complement(34232..34990)
                     /locus_tag="VYO12_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1586835.1"
                     /translation="MEESKELNTVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(35121..37100)
                     /gene="pknB"
                     /locus_tag="VYO12_000165"
     CDS             complement(35121..37100)
                     /gene="pknB"
                     /locus_tag="VYO12_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1586836.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKTPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPIQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(37097..37837)
                     /locus_tag="VYO12_000166"
     CDS             complement(37097..37837)
                     /locus_tag="VYO12_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1586837.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(37852..39165)
                     /gene="rsmB"
                     /locus_tag="VYO12_000167"
     CDS             complement(37852..39165)
                     /gene="rsmB"
                     /locus_tag="VYO12_000167"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1586838.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(39158..40093)
                     /locus_tag="VYO12_000168"
     CDS             complement(39158..40093)
                     /locus_tag="VYO12_000168"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1586839.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGCDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPKEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(40106..42502)
                     /locus_tag="VYO12_000169"
     CDS             complement(40106..42502)
                     /locus_tag="VYO12_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1586840.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYRHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(42568..42882)
                     /locus_tag="VYO12_000170"
     CDS             complement(42568..42882)
                     /locus_tag="VYO12_000170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1586841.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(42907..43533)
                     /gene="gmk"
                     /locus_tag="VYO12_000171"
     CDS             complement(42907..43533)
                     /gene="gmk"
                     /locus_tag="VYO12_000171"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1586842.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(43663..45276)
                     /locus_tag="VYO12_000172"
     CDS             complement(43663..45276)
                     /locus_tag="VYO12_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1586843.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(45545..45775)
                     /locus_tag="VYO12_000173"
     CDS             complement(45545..45775)
                     /locus_tag="VYO12_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1586844.1"
                     /translation="MEGVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMDKIRAGH"
     gene            complement(46013..47110)
                     /locus_tag="VYO12_000174"
     CDS             complement(46013..47110)
                     /locus_tag="VYO12_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1586845.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQSMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(47120..47866)
                     /locus_tag="VYO12_000175"
     CDS             complement(47120..47866)
                     /locus_tag="VYO12_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1586846.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKEKKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(47969..48322)
                     /gene="rsfS"
                     /locus_tag="VYO12_000176"
     CDS             complement(47969..48322)
                     /gene="rsfS"
                     /locus_tag="VYO12_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1586847.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(48335..48835)
                     /locus_tag="VYO12_000177"
     CDS             complement(48335..48835)
                     /locus_tag="VYO12_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1586848.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(48836..49429)
                     /gene="yqeK"
                     /locus_tag="VYO12_000178"
     CDS             complement(48836..49429)
                     /gene="yqeK"
                     /locus_tag="VYO12_000178"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1586849.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(49429..50058)
                     /locus_tag="VYO12_000179"
     CDS             complement(49429..50058)
                     /locus_tag="VYO12_000179"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1586850.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVAYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(50110..50421)
                     /gene="yhbY"
                     /locus_tag="VYO12_000180"
     CDS             complement(50110..50421)
                     /gene="yhbY"
                     /locus_tag="VYO12_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1586851.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(50635..51741)
                     /gene="yqeH"
                     /locus_tag="VYO12_000181"
     CDS             complement(50635..51741)
                     /gene="yqeH"
                     /locus_tag="VYO12_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1586852.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPSLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(51744..52334)
                     /locus_tag="VYO12_000182"
     CDS             complement(51744..52334)
                     /locus_tag="VYO12_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1586853.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(52286..53194)
                     /locus_tag="VYO12_000183"
     CDS             complement(52286..53194)
                     /locus_tag="VYO12_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1586854.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(53251..53811)
                     /locus_tag="VYO12_000184"
     CDS             complement(53251..53811)
                     /locus_tag="VYO12_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEV1586855.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQVLHFVPNRNITVVSNFSRTD"
     gene            53950..55161
                     /gene="sstT"
                     /locus_tag="VYO12_000185"
                     /pseudo
     CDS             53950..55161
                     /gene="sstT"
                     /locus_tag="VYO12_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            55229..56182
                     /locus_tag="VYO12_000186"
     CDS             55229..56182
                     /locus_tag="VYO12_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1586856.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            56325..56705
                     /locus_tag="VYO12_000187"
     CDS             56325..56705
                     /locus_tag="VYO12_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1586857.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            56804..57025
                     /locus_tag="VYO12_000188"
     CDS             56804..57025
                     /locus_tag="VYO12_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1586858.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            57042..57356
                     /gene="trxA"
                     /locus_tag="VYO12_000189"
     CDS             57042..57356
                     /gene="trxA"
                     /locus_tag="VYO12_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1586859.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
BASE COUNT        16312 a        13093 c        11087 g        17004 t
ORIGIN      
        1 ttgtcctatt cttgtttcat tttactatat ttccatgata aaacgcatag tatcaagttt
       61 ttttaatccc ctgatactat gcgtgtttgt aatttttaag attttgtgct tagagtcgac
      121 tccttatttt agatatttaa aaggaatctc acttccacag agccagttgt agacttggtc
      181 attgacaaat acattcatgg cttcgtgagc atactcaggc atgatacgat aggttttatc
      241 gcaggtcaga cgattataaa tcgcaaactg ggtaatggga tagcaaacat cgtcgtccaa
      301 gcccgtaatc atcttaacct caccttggat acgatgggca agatttttga catcgatata
      361 ggcaagggtc gccatgattt cctcctctgt ttcatggaag gggtcgtgaa acttgaaata
      421 acggaaaagt tcgtcgtaag cctcgctagt attaccaatc tcaatcaccc gtctgaagtc
      481 tgacaagaag ggataaatgg caactgtttt ctgaattcga ggattgagcg ctgctgcaac
      541 tagagctaga gcccctcctt gtgaggcacc atagctagaa agacgcttct catcaacctg
      601 agacagacta gcaacaattt cgaccaactg gtaaatatcc agataaacat ccttataaaa
      661 gaggtggtcc cgaccttcca tagcaccacg gataatatgc cccttcacgg tatttcctaa
      721 aggagaacgc aagccgtctt gtgagtaacc tgactggccc cgcacatcca tggaaacaac
      781 accataacca gctacggtga agcccagcat gtcggcccag tcccagccac gtcccatata
      841 accatgaaaa tggaagatta gtggaacctt ctcctcactc tttggaagaa caatgcgtgc
      901 atagaccttt ccttccttgc ttccttcaaa tgttaactca tagcacttga cttgaggaat
      961 gtggaaatct ctttcctcca agtggtagga tggaggtgtg gaaacatttt tcacttcccc
     1021 atcccagaaa gcatcaaagt cttccggaac ctcatccctt cctctatagg tcttaatttc
     1081 ttctagcaaa gctggatttt tcatagcatg ctccttttat tttgtaatcg ttaccacaac
     1141 tgtagcatat ctatgtaatc aatgcaagaa agaaggataa atagaaagtg aatttagatt
     1201 ctttactttt aggatgatgg gctgaaagta gttttagaaa cgaaaaagag catttcgctc
     1261 tctcttctgt ttatactagt tttttgcttc cttcttttgg aaagtggttt ggtaatttac
     1321 tttaatcgtt actgtcatat gagagtcctc gtttcttttt gatgacttta gtataagagc
     1381 caaacctaaa aactagctta agttttcctt agagaaagct taatccagat gttctttctt
     1441 ttccagatga agggcaatca tgcgccactc tggatcttct tgccaagctt ctatagaact
     1501 aatgtagcta gcaacctttc ttgcttcttc taaaatcgga aaatcttcga taatatcagc
     1561 cacttggaac tctggaagtc ctgactgtct ggttccaaaa atctcaccag aaccacgcat
     1621 tttcaaatct tcctccgcaa ggacaaatcc attggtcgtt tctgtcatga tgcgcatgcg
     1681 gtctttccca gaatccgtct tgggattggc aacgagaaca gcgtaggact gcttgtcccc
     1741 ccgaccgaca cgacctctaa gctggtgaag ttgactgaga ccgaagcgat cggcatccat
     1801 gataatcatg acagtcgcat tgggaacgtt gaccccaacc tcaataaccg tcgtcgaaac
     1861 cagaatatcc gtctttctct ccttgaaatc ctgcatgatc tggtcttttt cgtcactctt
     1921 catcctacca tgtagaagag ccacctctgc cttgcctgca aaatgagtcg tcaactcctc
     1981 tgataaggca atggcatttt tcaaatctag agcttctgat tcttcaatca aaggagagat
     2041 gacataggct tgggaacctt tttgaatttc cccctctaac caagtcaaga cctgaggtag
     2101 ttgctcatgt ttgatccagc gcgtcacaat aggcttccga cctgctggca tctggtcgat
     2161 aatggaaaca tccatatctc caaaggctgt gatggcaagc gtccgtggaa tgggagtcgc
     2221 cgtcatcatg aggacatctg gattgtcacc tttttcccgt aaaatacgcc tttgccctac
     2281 accaaaacgg tgctgctcat cgataataat caaaccaaga cgagcatact ccaccccatc
     2341 ttgtatcaga gcgtgagttc ctataatcaa atcagcctca cccttggcaa tggtctccaa
     2401 gacttctctc ttttctgcag ctttcaagga acctgtcaag agagccagtt tcaaattggg
     2461 aaaaaggttc tgtaaactct caaagtgttg ctctgcgagg atttctgttg gtaccattag
     2521 ggcagcctga taacctgctg tcactgccgc aaacatggcc aagccagcga ctaccgtttt
     2581 tccactcccc acatctcctt gtaggagacg attcatgtgg tggtcggact tcatatcagt
     2641 taaaatttcc tgcaaactct tttcctgagc ttgggtcagg gcaaaaggaa gacttgcttt
     2701 aactgctgtc actttttcct gagaccaatc cagaaccaga ccacttccct gaactctatt
     2761 ttcagacttg agcgtctgca gctgcatttg gaaataaaag agttcctcaa acttgatacg
     2821 gcgaagagcc tgcttgtatt ctgccaaatc ctttggaaaa tgcatagctc ggactgcctg
     2881 acaacgggac atgagtttgt atttgtctag taaagactgg ggcagatttt cttctatcaa
     2941 gaggtccagt ccctgatcaa aagccgtctt gatgaccttg accagactgg cctgactgat
     3001 tccctgagcc agacgataga caggctggag gtcatcttct acctgagcca gaaccttcat
     3061 cccagtcaga ctagccttag cgcggtccca ttttccaaag acagcaaggg ttgctcccaa
     3121 ctctatttta tcagccagat agggctggtt aaagaaattc accgcaaaaa cgacctctcc
     3181 ctgcttgaga ctaaaacgca ggcgattgcg cttgaaacca taatactgga cactagcagg
     3241 agtcactacc tgaccagaaa gaactgcctt ctcaccgtct tccagctcca gcacctgctt
     3301 ggttttgaag tcttcataac ggaaaggaaa gtagagcaag agatcttgca agttttcaat
     3361 tcctagtttg gcgtattttt ctgctgactt tggtcccaca ccaggcaaga catgcaaggg
     3421 ttgatgtaga ttcatgctcc actcctttct tttctaataa tattctctcg gaatacggtc
     3481 gctgaggagg caaaccacct catagttaat ggttacgcgg taggtcgcta cctgagttgc
     3541 agtgatttcc ttatccccat tggagccaat caaggttacc ttggttccta gcggataaag
     3601 cttaggcaat cgaatagtga tttggtccat cgaaaccctg ccgacaattg ggcaagcttg
     3661 gccatctacc aagacagaga aattttgcat gtctcttgtc catccatctg catacccgat
     3721 tggcacggtc gcgatgactt gctcgctatc cgcttgataa gttgctccat agcccatgca
     3781 agctccagct ggaactgtct tgacatgaac cagagcagac tccaaggtca aggccggtat
     3841 caaatcataa ggcaaatcca agaccgctcc acttggattg aggccataca tggcatctcc
     3901 catacgaacc gcattgaaaa tagtctctac atgccaaaga gtcgttgcag aattgctagc
     3961 atgaaccagc tctggaactt ccttcatact agctaaaata gttttaaacc gttctaactg
     4021 ggcattaaaa tagtcatctg attcctcatc agcagtagca aagtgggtaa agattccttc
     4081 aacacgaaca ccgtgttgtt gtagcaaatc ttgagcctgc tcaacctcac tggcctctct
     4141 aaaaccaatc cgtcccatcc ctgaatcaat cttgaggtgg actgtcaatc cagttaggtc
     4201 cacttcctta tctaagagtg cttgaatcca ctccagtcca gccactgtca aggtgaagtc
     4261 atattcttta gctagagcaa cagcttcgat ttcagaaact cctaaaatga ggattggctt
     4321 gctgagtcca gcttgtctga gttcaatggc ttcatcgata ttggaaacgc aaaagccatc
     4381 aacatcatct tgaattgcct tggcaacggc aacagctcca tgaccataag cattggcctt
     4441 gaccacagcc aacttgagcg ttccttgagg gatatgagcc cccatttgct gaatattttg
     4501 tcgaatagct cccagatgaa tcagagcctt ggttggtcta tgtggactag ctttcatgat
     4561 tttcctccaa aatgacactg gctgtcacaa actgatcggt gtggctgata gacagccaaa
     4621 tctttcctga aaatggtgcc tgactaaaat aaggcgcccc acgttcattg ttcaagactt
     4681 ccaaatcctg aaaaccgagc ttgctaatgc ccgttcccat agccttggaa aaggcctcct
     4741 tagccgacca gcgaccagct aaatattcta tttgcctgcg tcctttgaga ctggtgaagc
     4801 gctccatttc ctgagcggtc agtacacgct tagcaaatcc ttcatgtcgt gtaactgcgc
     4861 tttctatcga agccaattct tcgatgtcaa ttccgtgtcc aactatcatt ctcatcaaaa
     4921 ggagttgaga ttccccaact ccatcctttt cacttatttt gtcaaggtaa cataaatctc
     4981 ttctaccaag gcctctgtat tttcccaacc taggcaaggg tcagtaatag agcaaccaaa
     5041 gacctctggt tggttttgac gaccatctgc taggtaagat tcaatcataa atcctcgaac
     5101 cgtcttttta attttctcat tccaatcacg attctgcaag gtctggcgaa caattcgaat
     5161 ctgctccata tattgcttgc ctgagttatc atggttggtg tcaatgagga taaaaggatt
     5221 ttcaagttcc atggtttcat agcgttcaat ggcttggagt aggttttcat agtagtaatt
     5281 cggcatataa ttgccatact cgttgactgc tccacggagg ataacatggg ccaaaggatt
     5341 acctgatgtc tcaacttcct gcccatgata aaggaaggtt tgcttgtttt gagcagcata
     5401 gatggcgtta aacataacac ccaaatttcc tgaggttgga tttttcatcc ctactggtgc
     5461 atcaatccca gaagccacaa agcggtgctc ttggtcttcc acagaacgag ctccaacggc
     5521 atggtagctg accaagtcat ccaccaagat cagatttgac ggataaagca tctcatctgc
     5581 cgttgtcaaa ccagtctctg taatcacgcg gtagtgcaac tggcgcacag cctgcaagcc
     5641 gttaatcagg cttggagcct tagaagtatc tggctggtga accaatcctt tatagccgtc
     5701 tccattggta cgaggcttag cagtatacac gcgcatgacc atgaaaatct tgtccgctac
     5761 cttcttttgc aaggcggata aacggcgagc atattccaag accgcctctt cattatcaga
     5821 agagcaagga ccaatcacca agagaatacg gtcatcttcc cctgaaataa tatctgccaa
     5881 ttccctgtca cggctttcct tgagtctcaa ggcttccgca gacaattggg tttcagcctt
     5941 gatgacttcc atatcgattt cttgaccttt ttcaataaat gccatcttat tctcctaacg
     6001 ttttgtaaat ttctctgaca agagcttctg tgttatccca acccaggcaa gggtctgtga
     6061 tagacttacc aaatacttct ggctcatttt gtcgaccatc ttccagataa gactcaatca
     6121 taaaaccacg aacgaactgc ttaatttttt cattccaagc acggttaatc aaggtctggc
     6181 ggacaattcg gatctgttca atatactgct taccagaatt gtcatgattg gtatcaatga
     6241 tgataaaagg attttccaag cccattttct catactgggc aatggtatca attaaattgt
     6301 cataatagta gttgggaata ttttttccat actcattaag agcaccacga agaatagcgt
     6361 gtgaaagcgg gttcccagtt gtttctactt cttttcctaa gaaaaggaaa ctttgtttgt
     6421 tttgagcagc ataaatccca ttaaacatga cattgagatt tccagaggtt ggatttttaa
     6481 aaccagtaga aaatcctgcc ccacttgcca caaagcggtg ttgctggtct tcaactgaac
     6541 gggcaccaac tgccatgtaa gaaatcaaat catctacaag cggaaggttt tcaggataaa
     6601 gcatttcatc agcagttgtc atccctgttt ctgtgatgac acgatagtga agatggcgaa
     6661 cggctttgat tccattgata agactaggcg cttctgtcgc gttaggctgg tgaatcaagc
     6721 ccttatagcc atctccgttg gtacggggtt tggcagtata aacacgcata accataaaga
     6781 tacgatctgc cacttcttct tgtaggactg ccaaacgctt agcgtattca aggacagctt
     6841 cttcgttgtc agatgagcat ggcccgatta ccaagagaat tcgctggtct tctccacgta
     6901 taatggcttc tagctcttga tctcgctgtg atttcctctc caaagcctga ccttctaatt
     6961 ttgacaaggc acgaacttct tctatattta ttttagagct ttttgctgta aataccataa
     7021 ctcatctcct tataaagcaa acggataccc agtaaaaatt tacttttcac aagatatccg
     7081 cctctaaact atctcatttt attgtctttt accgtgacag tttttaaatt tcttaccaga
     7141 accacatggg caaagttcat tgcgtccaat ctggctcaaa tccaaatctt ctggcatact
     7201 tgcttggtga gcagcgatat tgcgagtcgc tgttgtactg atatgacgtt ctgcctgtgg
     7261 tctttcttgt tcatgaattt gtgctttcat catcaagcgt gtcacatcaa actcaatcga
     7321 accaatcata tcattaaaca tacggaaacc ttctgcctga tactcaacaa cagggttgtt
     7381 ctgagcatag ccacgaagtc caaccgcgtt acgcaattga tcaagggcat cgatatgatc
     7441 tgtccacttg ttatccacca ctcgtagaat caaaactttt tggaattctt taactgcttc
     7501 ttcatcgcgt agttttgaaa cctgactatc gtaaaccttc aaggcacgtt ggaaaagctc
     7561 ttccttgatg gccttatcag acaagcctga caagtcttcc atcgtaatag aatcttcagg
     7621 aagcaagttg tacttagcaa agttcaaaat tgcctctagt ttttcatctt gtttggcacg
     7681 cgcatgacca tcaacgacac gttcaatcgt gcgtttgatc atagactgaa tttcaggtgc
     7741 caagtcacga tctgcagtga tgacatcgta acgttgagca tagataatct cacgttgttc
     7801 acgcatgaca tcatcgtatt gaaggacttg tttacgggta tcgtagttat ttccttcgac
     7861 acgtttctga gctgcttcaa cctgacgcgt caacatgcga gactcaatcg cctcttcaga
     7921 catgttcaag cgttcaaaga ttcccttcaa gcgttcagaa ccaaaacgtt tcatcaaatc
     7981 atcttcaaga gataggtaga attgtgactc acctggatct ccttgacgac ctgaacgtcc
     8041 acgaagctgg ttatcgatac gacgactttc atgacgttct gtaccaataa cacaaagtcc
     8101 tccaagttca cgaacacctt caccaagctt gatgtcggta ccacgacccg ccatgttggt
     8161 tgcgatggta acggcaccac gttgaccagc attcatgatg atttgggctt ctctatagtg
     8221 gtttttggca ttcaagactt cgtgaggaac accagctgca accaatttct tagaaatgta
     8281 gtcactagtt tcaaccgcta ctgtaccaac caagacaggt tgaccctttt ggtaacgagc
     8341 cttaacgtct tcgacaaccg ctttaaactt agattctata cttgcataaa gaaggtctga
     8401 gtggtcaata cgttgaacag gacggtttgt tgggattgga ataacacgaa tgttgtagat
     8461 ttcacggaat tcttcttcct cagtcttacc tgtacccgtc ataccagaca atttcttgta
     8521 catacggaaa aggttttggt acgtgattga ggcagatgtc ttggtttcat cctggattgg
     8581 cacaccttct ttggcttcaa tagcttggtg caatccatca gaataacgac gaccttccat
     8641 ggtacgacct gtaaattggt cgacaatcaa gatttcttgc tcttcgctca ccacatagtc
     8701 aatatcgaga agcatgatgt agttggcacg aagggcgtta tcgataaagt gggtcaaagc
     8761 cacgttttcg atgtcataga ggttttcaag tttgaagtag ctttcagccc tgtcaatccc
     8821 tgaatcagac aaaccaatag tcttagactg cacatcgatg atgtagtcat ctttgttcaa
     8881 agattttaca tagtggtctg ccatgtgata caactgactg gtttcaaccg cattggcacc
     8941 tgatacaatc aaaggtgtac gagcctcgtc aatcaagata gagtcaacct catcgaccaa
     9001 ggcatagtta agcggacgtt gtaccatgtt ttcagcgcga acgaccatat tgtcacgaag
     9061 gtagtcaaat ccgatttctg agttagttga gtaagtaata tcacactcat aggcttcttt
     9121 tttctccatt ggagatttgg tagccaagtt aatccctact gacaaaccaa gccaagagta
     9181 caattcaccc atctcagtcg cgtcacgttc tgacaggtat tcattaaccg taactacgtg
     9241 aaccccttta cctgaaaggg cattgaggta taccggcatg gtcgcagtca aggttttccc
     9301 ttcccctgta cgcatctctg gcacgtcacc atggtgaaga acaatccccc ccatgacctg
     9361 aaccttataa gggaagagac ctaggacacg tttggcacct tcacggacaa ccgcaaatgc
     9421 ttcgtaaagc aatgaatcca gtgattctcc attttgataa cgttccttaa attcaactgt
     9481 ttttgctttt agttggtcgt cagtcaaagc agccatttgg tcttcgtatt tgaaaacctt
     9541 gtcagccatc ttttccagac gacggatttc tcctttatca ttttcgataa ttgtttttaa
     9601 aatattagcc atgtttttcc ttactttaaa ttccgaatat tttagaatgt tcctttaatt
     9661 ttagcacaat tactgattat tttcaaggaa gaatccccat tttgaaaagg aaaaagaggt
     9721 tagttttcaa gctaacctct ccgactttta tgatgcttta attgccaaaa atcggcaaaa
     9781 tggcaaatgt gataaataag ttaatccaaa gagaaagaca gcaaatcagt ccaaaaacaa
     9841 agagactgac tttcttggac agcaaggcca tcaaaatgga cagaattcca aaagctagca
     9901 agattttctg tatgatgaag atgtcaatcc ttacccccag aatcgctcta tcccaaggga
     9961 taaagaagaa agtcagccag atcagcagaa ctaaaccaat ataaaccttg gaatagcgtg
    10021 taataaatga ttttagatgt gccataagcc atctcctagg attgtatatc ctttacatat
    10081 tgtatatgtc cattttcgac agctaataca tctgtacaaa ggtctgaaat atctttcgat
    10141 atgtgcgacg tcaggataac cagaccattt tcttttttca ggtaagataa aatgacctgt
    10201 ttggttaacc tcacactctt ctcatccaaa gcattcatag gttcatcgag aaagagtata
    10261 gaaggagagg aaataaaggc ttgaatcaag gccatttttt gctttgttcc caaggataaa
    10321 tgacggtatt caacgtcttc aaattcctgt aaatcatagt attgaatcca ataggcaatt
    10381 cttttttccg taactttaga tgaaaaatac cttaacagtt ccaaattttc tctcagggat
    10441 aaatgagata aaaactcgac tttttcaatt aaaattcctg catcctgaat ataatgattt
    10501 tttaccccgt aaactttacc atcttgaaga acttttcctt tgtcaagctt aatataacca
    10561 gcaagtatct ttaaaagaac cgtcttgcca gatccattat tacctttaag tccataaatt
    10621 tttcctgatt gaaacaccaa attcaactga tttaaaatag gtcttttccc ataaattttc
    10681 tgtccattta taatttcaag tctcatcgtt tatcctttct taaatttctc ttccaagcga
    10741 ctaagaatca acataactaa taagcttaag agtactaaat acactgttac aatatgatcc
    10801 atcatccatt cttgtcccac aaatgccaaa aaatagagaa aaaaagaaaa accagcgccc
    10861 gcactaatca gtgctatcag ataagacagt agtaagtaca acatcattaa ctgagcaagt
    10921 aaaaagaaaa gtgcgactgt tttgaaatga attcctagag gaagtaaacc aagaaaccag
    10981 acaaacaaat cttgagtaaa aaatttagaa atgacaacca atcgtttcct tgtataatcg
    11041 aaaaaggagc ttgattgata gcgaatcaag tgatggtaat ggtctgccaa atcactttca
    11101 agtaaaatga cagagagcca tcctaccatg aaaagcgaaa ggtttaacaa gagtttgggg
    11161 atttctaaat tcacttcaga atagtcatta taggaaaaaa ttgaaatcaa ttctctcttg
    11221 aaaaagataa cagcatcctg cttttgaacc gctgttgaca atagatggtt tatcaaaatc
    11281 atcatcagaa ctatacgaag tagtaaaaca atctttctct ttagcaaaat attgttaaaa
    11341 ttcttaaaca ttatcttcat atttttcaag caccatgtaa gggacaatat aaaggatata
    11401 aggagtaaag agtttcatca aacttacatc cccatagcta gcttgcatca aactcgtcat
    11461 aggaaccata tagtaaggca aggcactata gagcagcata gaaccaagcc aaagaaacat
    11521 caaataggtg attgccgaac gagtcacatt tcctaaataa tcaactattt gtaaaaaata
    11581 gactgcattg atgaaaatac cgattagtag gacacaagta aagaacaaat agcttgttat
    11641 ctctccatct aggagccttt gtaaactact cccatcagaa aatagagaat tccatccaag
    11701 aggagaaaaa gtcccaaaga aatgagttaa aattgcaatt atcagcacag tcactaaata
    11761 tatcaaacaa gggatacttg caacttgcaa agtcaacttt ctttttaacc cttgataact
    11821 cacttctctt ccaatactca atcgtaaaac tttatttttc agctcaatat attgaaaacc
    11881 tagcaaaaca atgatgattg gaataataaa tgattgataa aaacctagat tgaactggag
    11941 aatacgaaaa tctaatgctg attttaataa gtcattttta attgcttgtc catcgaattt
    12001 agctagatcg ggcgcatgaa tatcaaaaaa gaaataagtc atcaaaacat cacgataaac
    12061 caaagcaaga aaaagcaaag taagcaagac ataaacaaat ttatgccttg ccaaagttaa
    12121 gagaggaatc ttcataagcc acctcctaca aatagaaagt aatataatac tcaatgaaaa
    12181 tcaaagagca aactaggaaa ctagccgcaa gttgctcaaa gcactgcttt gaggttgtag
    12241 ataagactga cgaagtcagt tacatatata atccaaggcg acgttgacgt ggtttgaaga
    12301 gattttcgaa gagtataaat caatgccttc cgcctttaga cttccctagt tcccgtctca
    12361 agtcaagcat ttttttgaaa cagatatagt ctaaccagtc actagcaaaa gcgataagta
    12421 gtaataatag ccaaataccc cataacttga tgtctgccac atttctcaag acgatattga
    12481 aaaacagaac tgaaaaaact gtccaagcaa ggctaaaaag agcatagagg gtgatgtaaa
    12541 tccagtaaaa atagtaaaaa attgggaaaa atttactttc tctattatcc ttgtcaatcc
    12601 agctgaaaaa gtaaaaacag ggtaataaga ttatcatttt aaacaagtgt tccatcacaa
    12661 gcctcctttt ttaaattgtt ttctttagtt tcgttctatt aaatgttatt accaatataa
    12721 accttggaat agcgtgtaat aaatgatttt agaggtacca taagccacct cctacaaata
    12781 gaaaccgata taaatcaatg ccttccaccc ttagacttcc ctagttcctg tctcaagcga
    12841 aacatttctt tgaaacagga ataagttaac caattcatac caatagctag cagaataaaa
    12901 agaaaccaaa tgccccataa cttgatatct gtcacatttc tcaagacggt attgaaaaac
    12961 agaactgaaa caactgtcca agcaaggcta aaaagagaat agaaggggat gtaaaaccag
    13021 taaaaataat aaaaaattgg aaaaaactta ctatttctgt tggccttctc aatccagtta
    13081 tcaaaataaa agtacggtgc taaaagtaag aatttaaaca aatgttccat caccgacatc
    13141 accctccttc ttttgatagc gttttctatt attttattat atcaaaaaaa tccggaactg
    13201 tcattccaga ttctactttt ttatttgcgt tttcttgcga tgagatgaat cggtgttccc
    13261 tcaaaaacaa aggccttgcg gatttgattt tccaagaaac gcaggtaaga aaagtgcatg
    13321 agttcttctt cattgacaaa gatgacaaag gttggtggtt tggttgccac ttgggtcgca
    13381 tagaaaatct tgagacgttt tcctttgtct gtcggtgttg ggttgatggc aatggcatcc
    13441 atgatgacat cgttcaagac agctgatgga atacgtgtat tttgactttc gctgatttgc
    13501 ttaatcatct caggaagttt gtggagacgt tgcttggtta aagctgatac aaagataatc
    13561 ggtgcgtaag gcaggtattg gaactgctca cggatatctt cttcccagtt tttcatagtg
    13621 tggttatctt tttcaagcgt atcccacttg ttgaccacga taatcatccc tttaccagct
    13681 tcatgggcaa atcctgcgat acgcttgtcg tactcacgaa tgccttcttc cgcattgatg
    13741 accatcaaga ccacatctga acggtcaata gcacgcatgg cacgcataac agagtatttc
    13801 tcagtatttt cataaacctt accagactta cgcataccag ccgtatcaat catggtaaac
    13861 tcttgaccat ctgtatctgt aaagtgggta tcaatggcat cacgagttgt tccagcaaca
    13921 ggactagcaa taacacggtc ttctcccaag atagcattga tcaagcttga ttttccaacg
    13981 ttaggacgac caatcaagct aaacttaatg acatctggat tttcttcctc atattcattt
    14041 ggaagatttt ctacgatcgc atctagcaca tcccctgtac cgattccatg gacagatgag
    14101 ataggcaatg gttcacccaa accgagagca tagaaatcat agatatcatt tctcatctca
    14161 gggttgtcca ccttgttgac tgcgaggata actggtttgt gggtcttata aagcttacga
    14221 gctacgtatt cgtctgcatc agtaattcct tccttaccag acacgacaaa aacgataaca
    14281 tctgcttctt ccatggcaat ttctgcctgg tgcttgattt gttccatgaa aggagcatcg
    14341 acatcatcaa ttcctcctgt atcaatcatg ctaaaagaac gattgagcca ctcacccgtt
    14401 gcataaatac ggtcacgtgt cactccttcg acatcttcta caatggagat tcgctcacca
    14461 gcgatccgat taaatagggt tgatttccca acattgggac gtcctacaat ggcaatagtt
    14521 ggtagggcca taatttctca ctttctacaa taatttcttc tgttcaagat tttttctagt
    14581 tgagcttggt tcagcttgac caaactgttc tgctaggcgc tgactccagc ttgtggtcgc
    14641 acgcgcccca gcatagtcag cctgaacacg gtcataagct tggattgcct cagttgactg
    14701 ttcttggtat tcttcctcaa agacaacatt ctctagtggc agtcttggtt tcatatcatg
    14761 atgttgattt ggcacaccca gtgccatccc aaagacagaa taggtgtagt caggtaggtt
    14821 aaagagctct gccacttctt cagacttgta tcgaaccaaa ccgataatca caccaccata
    14881 gcccaagctt tcagctgcca acaaggcgtt ttgtccagca agagctgcat cgaccgaact
    14941 aatcaagaga ccttccacac cttggggttg gaaggtgtcg gtatgaagtc gggctccctt
    15001 ttctgctcgg ttcaaatctc cgacaaagag aaggaaaaca gcagactggc gaatggcttc
    15061 ttgaggtacc aattcataca aggcatcttt cttctcttga cttcgtacca caatcacaga
    15121 gtaggattgg aaattcttcc aagatgatgc catctgggct gctgtcaaaa tctcatttaa
    15181 gtctacttgg ggaatttctt gctctttaaa cctgcgcact gaagtatgag ccttcatcaa
    15241 tttaatggtt tctgtcatcg acggtttact ccttctaaac gagtctcctc agccaaataa
    15301 cggatgcgtt ccatgacccg tctggcttcc caggtttcgt catttccatg tttcactttc
    15361 gcaaaatgct tctccaaatc ttcaaagttg aagttggatg tgaaaaaggt cggtaaattt
    15421 tcctgcatcc gatattggag aatgacctgc aggatttcgt cacgcaccca aacggttgat
    15481 tgctcggcgc caatatcatc taaaatcagg acctcagaca gcttaatctc atccaccaag
    15541 gtcttaacat tgccatcact gatagcattt ttgacatcaa tgacaaagct aggatagtgg
    15601 aggagagtgg atgatacacc acgtttttct gataaatcat gagctagggc agccaccatg
    15661 aaacttttac ccacaccaaa gtctccatac aagtaaagac ctttcaaatt attggggtaa
    15721 tcgttaacaa attctatcat tctttttaga acagttaagc gctgaacatc atcacgataa
    15781 acatctaaaa aactgacatt tttcaggcta gatggaaaat taattaagtt gagacgtttc
    15841 ttgatagccg cttctttttc agctgcgatt agttcaggag tttcttcata agaaacatct
    15901 gcatagccat ggttcatgac taatataggc ttgtagcctt tggcaatata atctgtatct
    15961 cctcggagga atttatctct ctcagtgatg tattggttaa acttggaaat actacggttc
    16021 aactcgtcct gattgaggga ttcttgctgg acaaaggccg caacatcagg atccttcatg
    16081 attttctgaa ctaaatcttg atagtgaaaa cgacttggtt gacgtttgag tacatctccg
    16141 acactttcca tctaatcacc tccgttatct aattttgcta ataattctag ggtttggcgt
    16201 tccatctctt ctaattcctc agcggatgac gtatttttat attcaggatt actccattta
    16261 ggaatattgg tttttgctgg ggcagtctga ttctgttttt gtgtttttgc tttctgttct
    16321 cgctcacgga tgcgcaagac tgccttttct gccgaatgaa tcttttgata ggcatagtca
    16381 ttggctacct tcatggcata tttctcattg atatttgccg aatcgacctt attaaaggtc
    16441 aataaaagaa tgatattgat gacttcgtcc agcaagccca agccagccat ctgttgcaag
    16501 agttctcttt ctgtttgggt aatggttccc ttgcgtgttt gcttgatttc tgccaagaac
    16561 tgcagggcag ttttactttt ggcttctttg ataatggttg tttccttaag actaaagtca
    16621 gaggaaactg gtttttgagc aattttttcc cgcatgcgtt tggttgaaat aacctgagaa
    16681 acagctgttg acttggccaa ttgataggtt tcaaaccaag tccatttctt ctcctcggca
    16741 atagcaaaga ggtttaagac atcggactgc tcatccgcaa aacgaagtcc atctcgagcc
    16801 atcagctggc gaaaatggtc caagtcaaaa tcgttggcca ctttcttctt gagaccaagg
    16861 tcttcttgac tgcctagttc tgccaattct ggaaagactt gattgagtga gacaggtatt
    16921 tcttcaccat cagcactttc aactttcaaa tcctccacag ctacatcgcc aatctttttc
    16981 tctaagagtc tgcgataaac aggatgctcc aagaagtctt gactagatag aggagcatgg
    17041 aggactagct gataaacatc ccccttttga tagagggtca agagattaaa agcagataag
    17101 attttcaatg attttatcag tctatccatc ccaaagttaa gatggttgag aatgcttgaa
    17161 aaaagatatt cctttctacc attatcccaa aaactgattg tataaagata aaggctcagt
    17221 gcctcctgac cgataatcgg gaggtagcac tgtaccagag atgaggtatc ttgcgacacc
    17281 cgattattct ttagataaga aaaacggtca attggcttca tttatctttc ctttttcttt
    17341 ttagaggact gggtgatttg ttggagcaag ctctctaact cactgacatc cttaaaacta
    17401 cgatagacac tagcaaaacg tacataggta atctcgtcca attcagccaa ctcctccatg
    17461 acgagtgaac caatgtcctc actttgaatt tcattttcat ttcgaccacg gagtttctgt
    17521 tcgatacgat tgactaccat gttgatttca tcacttgaca caggacgttt ctgggctgag
    17581 cggataatcc cattaaagat tttatctctg gagaattgtt cccgtgtgcc atctttttta
    17641 acaaccacta aggttctttc ttctactcgt tcgtaggttg taaaacggtg ttggcattcg
    17701 tcgcactcac gtcttctacg aatggtgttc ccttcttctg cttggcgact atcgataaca
    17761 cttgacttgg tagccccaca ttttggacaa cgcatccttt ccctccttat cgttttcttt
    17821 tcattatacc attttttaag cgattcccaa aacaattctt ctttttgctt gacaagtttt
    17881 ttgttttgtt gtattattta attaagacaa aagggtaaaa gaaaggagac taagatgtcc
    17941 tggacatttg acaacaaaaa acccatctat ttacagatta tggagaaaat caagcttcag
    18001 attgtttccc atacactgga acccaatcaa caacttccaa ccgtgaggga gctagctagc
    18061 gaggctggtg tcaatcccaa taccatccaa agagccttat cagaccttga acgagaagga
    18121 tttgtctaca gcaagcgaac aactggacga tttgtgacta aggataagga gctaatcgct
    18181 cagtcacgca aacaattatc agaagaagaa ttggaacact tcgtttcctc catgacccat
    18241 tttggctatg aaaaagaaga actaccaggc gtagtcagtg attatattaa aggagtttaa
    18301 gcctatgtca ttactagcat ttgaaaatgt atccaaatca tatggagcaa caccagccct
    18361 tgaaaatgtt tctcttgaca ttccagctgg aaaaattgtc ggccttcttg ggccaaacgg
    18421 ctcaggaaaa acaaccctga ttaaactaat taatggcctc ttacaaccag atcaaggacg
    18481 tgtcctcatc aacgacatgg acccaagccc agcaaccaag gccgttgtag cttatttgcc
    18541 tgatacgacc tatctcaatg agcaaatgaa ggtcaaagaa gccctaacct acttcaagac
    18601 cttctataaa gatttcaatc ttgaacgcgc ccatcatcta cttgcagacc tgggcattga
    18661 tgaaaatagt cgtctcaaga aactatcaaa aggaaacaaa gaaaaggttc aactgatttt
    18721 ggttatgagc cgtgatgctc gtctctatgt tttggatgaa cccattggtg gggtggatcc
    18781 agcagcccgt gcttatatcc tcaataccat tatcaacaac tactcaccaa cttctaccgt
    18841 tttgatttct acccacttga tttctgatat cgagccaatc ttggatgaaa ttgtcttcct
    18901 aaaagacgga aaagtcgtcc gtcaaggaaa tgtagatgat attcgctacg agtcaggtga
    18961 atccattgac caactcttcc gtcaggaatt taaggcctaa gcaaaggaga ttatttatgt
    19021 tttggaattt agttcactac gaatttaaaa atgttaacaa gtggtattta gccctctacg
    19081 cagccgtgct agtcctttct gccctcatcg gaatacagac acaaggcttt aaaaatctac
    19141 cttaccaaga aagtcaggct actatgctac tttttctagc tacagtcttt ggtggcttga
    19201 tgcttacact tgcgatttca accattttct tgattattaa acgcttcaaa ggtagtgtct
    19261 acgaccgaca aggctatctg actttgacct tgccagtttc tgaacaccat atcatcacag
    19321 ccaaactaat cggtgccttt atctggtcat tgattagcac cgctgtattg gctctaagtg
    19381 ctgttattat tctggcttta acagctccag aatggattcc tctttcttat gtgattacat
    19441 ttgtagaaac acatctccct cagatctttc ttacaggtat atccttccta ctaaatacca
    19501 tttcaggaat cctctgcatc tacctggcta tttccattgg acagcttttc aatgaatacc
    19561 gtacagcact cgctgttgca gcctacattg gtatccaaat cgtcattgga tttattgaac
    19621 ttttcttcaa tcttagttcc aatttctatg tcaattcact ggtaggactc aatgaccatt
    19681 tctatatggg agcaggtata gccattgtta aagaactcat attcatagct atcttttatc
    19741 tcggaaccta ctacatcttg agaaataagg ttaatttgct ttaaataatt tttacctaga
    19801 tatgtaacat actcatagaa caaaagagac caggcaaaaa gtctttaaaa ttagaaaacg
    19861 catagtatca agtgttaaaa atccttgata ttatgcgttt tttgtgagct gacttatttc
    19921 ctttcgctat atcgcaaaat gaaataagaa cggaacgatg ggattttgga attcaaatca
    19981 atttataaga atgttttaga agtaatatta tcctattcca gattcagttc actatacaat
    20041 tgagttttca agcaacctgt ttacataatg tgtacataat taggttcgtg attccaccct
    20101 tttcaccttt aaaaacctcg ctttcgcaag gctcttctat ttataagata aggcacgttt
    20161 aaaggttttc caaatcccta aatcatccgt ttgaagaacg agactagcat acatgcgtcc
    20221 gataaatcct gttgctacca ccgcaaaaat cactgtaata gcaagtgaaa tccatgcttc
    20281 tgctcccccc gcatagccat taatcgttcg aaacggcata aagaaggtcg aaataaaggg
    20341 aatataagaa ccaatcttca agaggagatt gtcaccagct gcacctagag ctgtcactcc
    20401 aaaaaaacca cccataatca aaatcatcaa aggcgacaag gctttccctg agtcctcagg
    20461 acgagaaacc atagatccta ggaaggctgc caagactacg tacatgaaaa gactgatcaa
    20521 aataaagagc aaggtattca gtgagatagc atctcccaag tgatccaaaa taccagactg
    20581 agccaagaat ggcaaatctt taaagagcaa aacggcagcc agaccaccta caacatagat
    20641 cccaatatgc gttaaaatca ctagaaacag agccatcatc cgcgcataga aatagtgact
    20701 tgcccttatg ctagaaaaaa cgacttccat aattttggtg cctttttcac tggcaacttc
    20761 ctgagctgtt acacccgcat aggtaatcag aatcatataa agaaagaatc ctaaggcacc
    20821 tgctgcaatt gtttgaataa actttttatt ttccttggct tcatcaatct tttctgtgaa
    20881 ttgaattgtc tgcgctaagc gtttttcctg ctcttgagac aaggaagcag ttgaacgatt
    20941 aagctgattt tgcagttcat tgagtgtacc tgtaacctca aatttaattc cattttcaag
    21001 cgatgtttcg ccatgataaa ctgcctttag aacactatct tcttgatcaa tggtcaaata
    21061 accttttaat ttttcttctt taattgcttc tttggcactt gcttcgtctt tatagtcgaa
    21121 gttaacacca tttacattct tcagtccttc tgctacagat ggcactgttg tcactactgc
    21181 cactttatta tttttagcca tagaagaacc ttggatatgc ccaattccta cagagattcc
    21241 taaaaagagg aacggcgaaa tcaccataaa gaagaaactc catgactcga catgtcgaag
    21301 ataggtttcc ttgattacaa cccacatatt tctcatactt ccactcctga ttctagttta
    21361 aagatttcat cgatagttgg cgcttgttgg tcaaatgttg cgatatattg accttgagtc
    21421 aagattggga agagttccct tccagcgctc tcatcctcca aaatcaattt ccaactgcct
    21481 tgtttggtca agctcacctg tttgacatga ggaagatttt ccaattcttc cttgcttcgt
    21541 tcacttgaaa caaagagacg cgttttcccg tattgattgc ggacatcctg aactggtccg
    21601 tgcaagacca cacggccatc tcggatcatc agaatatcgt cacaaagttc ctcaacattg
    21661 gtcatgacat ggtcagaaaa gataatggtt gctccgcgct ctttttcctg aaaaatgact
    21721 tgtttgagca attctgtatt aactgggtcc aatccactaa aaggctcatc caagataatc
    21781 aggtctggtt catgaatcag agtaataatg agctgaatct tctgctgatt tccttttgac
    21841 agactcttga ttttatctgt cagctttcct ttcacttcca acctcttcat ccattgaggg
    21901 agtttttctt tgacttcttt ggcatccatg ccttttagag tcgccaagta gcgaacttgt
    21961 tcaagaactg tcaatttagg catgagactg cgttcttcag gcagataacc aatccgagca
    22021 taggtctcct gacgaatatc ctgaccatcc agaccgattt ctccctgata ttctaggaat
    22081 ttcaaaatac tatggaaaat cgttgttttt ccagcaccat tttttccgac tagtcccaaa
    22141 atacgacctg gtcgcgcttg aaagtcaata ccaaacaaaa cttgcttgga gccaaaactt
    22201 ttctctagac ttcttacttc tagcatcttt cacctccgaa atttcttgca ctcattatac
    22261 tcctttttga tagcctttac aatgtttttg tccattttta gaagactatt gctgtgtaaa
    22321 atatggcctg gagcactttt atactcaatg aaaagcaaag agcgaactag gaagctagcc
    22381 gtaggctgct caaagtacag ctttgaggtt gcagataaaa ctgacgaagt cagctcaaaa
    22441 cactgttttg aggttgtgga tagaactgac gaagtcagta actatatcta cggcaaggcg
    22501 aagctaacgt ggtttgaaga gattttcgaa gagtattagt gataaatcca ttatacagta
    22561 gcaaactcaa tttatacctt ccgctcctca actgtctatt tttaatcctg aattgttatt
    22621 tgagtaactc ctttttcctc ataaagtttt cttcctctaa aacttctgga aaaaagcgaa
    22681 tagtttcaga caacattttt ataagaaaca agttcatctg tcatttcaag aaggagtaat
    22741 cctttatcta ctaatggacg gaacggaatt caaccgcttg tccgatatat tttctaagga
    22801 ttatatagta aaatgaaata aaaacaggac aaatagatcg ggacagtcaa atcgatttct
    22861 aacaatgttt tagaagtaga tgtatactat tctagtttca atctgctata tctattatgc
    22921 acaccactat aggatctaat aaaaatcaca acaagttcat tcatagatgg ttacctcagg
    22981 gaactaagaa aacgactaca aaggaagtcg cattcatcga aaagtggatt aacaactatc
    23041 ctaaaaaatg cttgaactac aagtccccca gagaagactt ctggatgact aacttgaact
    23101 tgaaatttag caataattaa ttcaccatct aactatattt agtaattatt tcagaactga
    23161 ttaatattaa aattaactaa caattcaaag gattcatact agccataaat tacgtccatc
    23221 agagagagac tcttactact tttagatttt agtctttcta gcttcagaat acatctaaac
    23281 tttagggaaa atgactattc gaaagcgcga atgcctcaaa attatctcag ataagctatt
    23341 cgaaacttag aatgctttta aatttatgga attgcgatta ttcgaaacct agaatgcata
    23401 taacctttag ttgacagacc tattctaagt ctcgaagggc tatttacttt ctattcctta
    23461 tcaaaaaaga ctcattcccc ctttctcctc caaaatatgg tatagtagaa atatactatc
    23521 tatgaggagt ttacatgtca caggataaac aaatgaaagc tgtttctccc cttctgcagc
    23581 gagttatcaa tatctcatcg attgtcggtg gggttgggag tttgattttc tgtatttggg
    23641 cttatcaggc tgggatttta caatccaagg aaaccctctc tgcctttatc cagcaggcag
    23701 gcatctgggg tccacctctc tttatctttt tacagatttt acagactgtc gtccctatca
    23761 ttccaggggc cttgacctcg gtggctgggg tctttatcta cgggcacatc atcgggacta
    23821 tctacaacta tatcggcatc gtgattggct gtgccattat cttttatcta gtgcgcctat
    23881 acggagctgc ctttgtccag tctgtcgtca gcaagcgcac ctacgacaag tacatcggct
    23941 ggctagataa gggcaatcgt tttgaccgct tctttatttt tatgatgatt tggcccatta
    24001 gcccagctga ctttctctgt atgctggctg ccctgaccaa gatgagtttc aagcgctaca
    24061 tgaccatcat cattctgacc aaacccttta ccctcgtggt ttatacctac ggtttgacct
    24121 atattattga ctttttctgg caaatgcttt gacacgtaaa aaatccgttt ggtttcccaa
    24181 gtggattttt aaagcgtaga ttaactatag cttgatacta aatatacttt ggtatggaaa
    24241 ttatgcatat ttttcgatag tgaggcgagg acttacctag cctttccgcc gtgatagaaa
    24301 cacctgaaat ctaatggttt caggtattcg gaaactttga gacactaggc tcaaagttta
    24361 ggtatggaat ttcgaagaaa gtcgctaccg tccgtaatca cttaaggaaa ggctcaaaaa
    24421 tattgttttc aaccacaaaa tccgtttggt ttcccaagcg gattttgtgc tttattttga
    24481 aacttctttt gcaagaacaa agttcccaag tgtggcagaa ccatttcctg cgactgctgg
    24541 cgtcacgata tagtcacgca catctggtac tggtaggtaa ccattaagaa gagatgtaaa
    24601 tttctcacgg acacggtcca gcatatgttg ttgagccatg acccctccac caaagacaat
    24661 cacgtctggg cggaaagtca ctgtcgcatt aaccgcagct tgagcgatat agtaggcttg
    24721 aacatcccaa acagggttgt tgagttcaat attttcccca cgtacacctg tacgagcttc
    24781 caaacttgga ccagctgcat aaccttctag acatccctta tggaaaggac aaacaccctt
    24841 aaactctttt tcaatatcca ttgggtgtct agcaacataa taatgaccca tttcagggtg
    24901 acccacacca ccgataaact caccacgttg gatgacgcct gcaccgatac ctgtaccgat
    24961 tgtgtagtaa accaagtttt cgatacgacc accagcattg ttacgggcaa ccatttcacc
    25021 gtaagcagag ctgtttacgt ctgttgtgaa gtacattggc acgtttaggg cgcgacgaag
    25081 ggcaccaagc aagtctacat ttgcccagtt tggttttgga gtcgtcgtga taaagccata
    25141 agtttttgag tttttgtcaa tatcaatcgg cccaaatgaa ccaactgcaa gaccagcaag
    25201 gttatcgaat tttgagaaga actcaatggt tttatcgatt gtttcgattg gagttgttgt
    25261 tggaaattgt gttttttcta caacgttaaa gttttcatca ccgacagcac agacaaactt
    25321 tgtaccgccc gcttccaagc ttccatataa ttttgtcatg ataaacctct tgtttttatt
    25381 ttctttatta tagcatactt cgaaagtcta aatgtctcta ttttttagat tttcctctgt
    25441 aaatcttact atctaataaa aacgaacaaa catgtcattt gttcgttttc acattagaga
    25501 ggattgatta gattttcact tcgatcacag catccccctt cgaaactgaa cctgttgcga
    25561 ctggagctac tgaagcgtag tcagctgtat ttgtaacgat aaccattgtt gtatcatcaa
    25621 gtccagctgc agcgattttg tttgagtcaa atgttccaag aacatcgcca gctttcacct
    25681 tattaccttg agcaactttt gcttcaaaac cgtcaccgtt catagataca gtgtcaatac
    25741 caacgtgaat caaaacttca gcaccatttc ttgttttcaa accaaaagcg tgccctgttg
    25801 gaaaggcaat tgaaacttca gcatcagcta gtgcatagac cacgccttga cttggtttca
    25861 cagcgatacc ttgtcccata gctccacttg agaagactgg gtcattgaca tcagcaagag
    25921 cgacaacatc accgacgata ggagttacaa gtgtttcatt ttgaagagct gctggcgcaa
    25981 cttcttcttt ttcttcagcc acttcagctt gttttgcagc tgcagttgcg tctacttcat
    26041 cttcgtaacc aaacatgtaa gtaagagcaa aaccaagggc aaatgataca gctaccataa
    26101 gaaggtattg tggaagttgt ccgttaccaa cataaagcat tgtaccaggg atgatggtga
    26161 taccattacc agtaccagca agtccaagga tagaagccaa tccaccaccg attgcaccag
    26221 caatcaatga aaggaagaat ggtttacgga agcgcaagtt caccccgaag atagcaggct
    26281 ctgtaatacc taggaaggca gaaagagcag ccgggaaagc aagtgttttc agttttggat
    26341 tttttgtttt aacaccaacc gcaacagtag cagcaccttg agctgtcata gcagctgtga
    26401 tgatagcgtt gaatgggtta gcatggtcag cagcaagtaa ttgcacttca agcaagttga
    26461 agatgtggtg cacacctgac acgacgatca attggtgaac cccaccaatc aagaaaccac
    26521 caagaccaaa tggcatgcta agaatcgctt ttgtagcaat aaggatgtag ttttcaacaa
    26581 cgtggaaaac tggtccaatg acaaagagtc caaggataga catgaccaaa agtgtcacga
    26641 atggtgttac caagaggtca atgacatctg gaacaacctt gcggacagct ttttcaaatt
    26701 tagctccgac aaccccgatg atgaaggctg gaagaacgga accttgcaaa ccaacaacag
    26761 ggatgaaacc aaagaagttc atcgctgtta cttcaccacc ttgagcaact gcccaagcgt
    26821 ttggaagtga gccagagaca agcatcatac caagaacgat accaacggca ggatttccac
    26881 caaatacacg gaaggttgac cacacaacca aacctggcaa gatgatgaag gctgtatctg
    26941 tcaagatttg tgtgtaagtt gcaaagtcac ctggaagtgg catttcaaga gcgttgaaaa
    27001 gaccacgcac acccatgaag agacctgtcg ctacgataac tgggatgatt ggaacgaaaa
    27061 catcaccgaa agtacggata gcacgttgga accagttccc ttgtttagca acttctgctt
    27121 tcatgtcatc cttagatgat gttggtaagc caagtacaac aacttcatcg tacattttgt
    27181 taactgtacc tgtaccaaag ataatttggt attgccctga gttaaagaaa gcaccttgaa
    27241 ctttttccaa gttctcaatc acttctttat tgattttctc ttcatctttg accatgacac
    27301 gtagacgagt cgcacagtgg gcaacactat tgacattttc acgtccgccc aaggcatcga
    27361 tgactttttt tgcaatttcc tgattgttca tttgcaaaaa tctccttata taacattttg
    27421 ttcttgtttg aaagcgattt tattcgccct tacgactatt attttatcat gttttaaaaa
    27481 tatgtcaagc gttttgcaga aaaaatattc tatccactta gtgggcaagt tttagattga
    27541 ttgtttttga ctgtttcatc aggaattcct tgaaaaataa gtttgaaacc ttggttaaaa
    27601 agtcttttat ttcattatct ttcacatttt cgtaaaaatt tgattgattt cctgtattta
    27661 ttttatgata atcgtttgac agtttttcct cttttttaac ataaaaggct tgcatttttt
    27721 atcgaaaaca gttactatta aaagtgataa gatttttgga ggaatgaatg gaatggacaa
    27781 ctgagcgtcg ttacagactt tatcaagatt ggacgcaaga agaaattcaa catataaagg
    27841 aaaatatggc acaatctcca tggcatactc attaccatgt tgagccaaaa acaggacttc
    27901 tcaacgaccc aaatggcttt tcttactttg atggcaagtg gatcctcttt taccagaatt
    27961 ttccttttgg tgcagcccac ggtttgaaat cttgggcaca gctagaaagt gatgatttgg
    28021 ttcactttaa agaaactgga atcaaagttt taccagatac tccattagat agccacggtg
    28081 cctactctgg ttctgccatg caatttggcg ataacttatt cctattttat acaggaaatg
    28141 ttcgcgatga aaactggatc cgtcacccat accagatcgg tgctttgatg gacaaggagg
    28201 gtaagattac aaagattgac aagatcttga ttgaccagcc agcagactct actgaccact
    28261 tccgcgatcc acaaattttt aactttaaag gtcaatacta tgctgtcgtc ggtggacagg
    28321 acttggagaa aaaaggcttc gtccgtctct acaaggctgt caataacgac tacacaaact
    28381 ggcaagcagt tggcgacctt gactttgcta acgaccgtac tgcctacatg atggaatgtc
    28441 ctaatctggt ctttgtagag gaacatcctg tccttctcta ctgtccacaa ggattggata
    28501 agaaagttct agactacgat aatatctttc caaatatgta taagattggg gcttcctttg
    28561 accctaaaaa tgccaaaatg gtagatgtgt ctcaacttca aaacatggat tacggtttcg
    28621 aagcctatgc aactcaagcc ttcaacgctc ctgatgggcg tgctctagca gttagctggc
    28681 ttggtttgcc agatgtttct tacccatctg accgttttga ccaccaagga accttctctt
    28741 tggtcaagga actcactatc aaagacgaca agctctacca gtatccagtc gctgctatta
    28801 aggaccttcg tgcttctgaa gaagccttct caaaccgttc ccaaaccaag aacacttacg
    28861 aacttgaact caacttggaa gctaatagcc agagcgagat tgtcttactt gctgataaag
    28921 aaggtaaggg actttcaatc aactttgacc ttgtaaacgg tcaagtgaca gtggatcgta
    28981 gccaggctgg agaacagtat gcccaagaat ttgggacaac tcgttcttgc cctatcgaga
    29041 atcaggctac tactgctaca atcttcatcg ataactctgt ctttgaaatt ttcatcaata
    29101 aaggagaaaa agtattttct ggtcgtgtct tcccacatgc ggaccaaaat ggtatcctga
    29161 tcaaatctgg aaacccaact ggaacttact atgaattaga ttatggtcgc aaaactaact
    29221 gatgtcgcca aacttgcagg cgtcagtcct actaccgttt ctcgggttat caataaaaaa
    29281 ggatatctat ctgagaaaac catccaaaaa gtcaatgaag ccatgagaga attaggctat
    29341 aaacccaaca acctggctcg tagtctgcaa ggaaaatcag ctaagttaat cggcttgatt
    29401 ttccccaata tttccaatgt tttctatgca gaattgattg ataaattgga acaccaactc
    29461 ttcaaaaatg gttacaagac catcatctgc aacagtgaac atgattctga gaaggaacgc
    29521 gaatacatcg aaatgttgga agccaatcag gtagacggca tcatttctgg tagtcacaac
    29581 ctaggaatcg aagactacaa tcgtgtgaca gcgccgatta tttcctttga ccgaaaccta
    29641 tcgccagaca tccctgttgt ctcctctgac aactatgctg gtggggttct tgctgcccaa
    29701 accttggtca agacaggtgc ccagtctatc atcatgatta cagggaatga caattccaat
    29761 tcgccaaccg gactgcgcca cgctggtttt gcatccgtac tcccaaaagc tcctattatc
    29821 aatgtttcca gtgacttttc tcccgtcaga aaagaaatgg aaatcaagaa tatcttgacc
    29881 cgggaaaaac cagatgccat ttttgcttcg gatgatttga cagctattct ggtcattaaa
    29941 atcgctcaag aattgggcat ttctgtccca aaagagctca aggtcatcgg ctatgatggg
    30001 acctacttta tcgaaaatta ctaccctcaa ttggctacta tcaagcaacc tttggaagag
    30061 attgcttgtc tcactattga tcttctcttg caaaagattg aaggcaagga agtcgccaca
    30121 actggttact tcttaccagt tacgctatta ccaggaaaaa gtatttaaac acaagaaaac
    30181 tcagaccgat tcgtctgagt ttttatgatc ttaaattttc gagatagcgc tgggctgtct
    30241 ctaggttaaa ggttttatct gagatgaggc gctctactag gggagcaact tcagattcac
    30301 tagccccagc taggagagct agggatttgg cctgtagttt catgtggcct tgctggatgc
    30361 ccgtacttac caaggctttg agggctgcaa aattttgagc aagaccgatg gacacgataa
    30421 tctgggctaa ttctctggca gaaggatttc ctagtagatc atgactgaga gctacacgtg
    30481 ggttgaggcc gatagagcca cccttagtcg ctacaggcat gggcagggtc atctcaccga
    30541 ccaattcttc tctttcaagg tccagcgtcc agcagctaag accttgatag cgtccatctc
    30601 gactggcaaa ggcatgggcc ccagcttcga tggcacgcca gtcattacca gtggcaatca
    30661 aaatcgcatc aataccatta aaaattcctt tattatgagt agcagctcgg taaggatcag
    30721 cctgcgcaaa ctgactagcc aacgcaattt tctccgcaat ctctcgtcct tgatcctttt
    30781 ggcggctcaa gtagcgaaag gcgatgcgac agcttgcagt caccagagaa tcagtcgcgt
    30841 agttggacag gattcccatg agactctgtc cctgactgag ttcttctaag actggtttca
    30901 aggcttccag catggtgttg agcatattgg cacccatggc ttcctgggta tcgacatgaa
    30961 tataaacaac gagaaagtct ggttcgcctt ttatctgctc gacatgcaga tcacgcgccc
    31021 cacccccacg tttaacgata gaaggatagg cttgattggc aagctccaag agctccgctt
    31081 tcttgctggc aatcttctct tgcgctagtt taggattagc aacttgataa agggctacct
    31141 gcccaatcat ctggcgctga tggacttgtg cagtaaaacc acctgcacgc ttgatgattt
    31201 tgctggcata gctggccgcc gcaaccacag agggttcttc tgtcacatag ggaacggtgt
    31261 attcctgacc gttgacaagt acctccggaa ccagtgaata aggcagagaa aaagttccca
    31321 ctacattctc actcagctgg tctgccacag tcacgctcat ctgttcatcc ttctccagac
    31381 tagcttgtct ctcaggacta aggagcgcct gagcttttaa cagctcgagg cgctcttggt
    31441 atgatttttt agaaaatcca ttccaactta tcttcattat ttttcaacct tgctataacg
    31501 gcgttggtgg tcgagaattt caaccaaggc aaaatcttga ttttcatagc cagcaaactg
    31561 ggcagagttt gtttcatcca agtttacttc ctcaaaaaag accttttcat agtctgcaac
    31621 ggatagggca gttcgttggt tgagcttgtt caaacggtct ttatccaaat aagcttcata
    31681 tccttcaacc aattcaccac tgaagaactc agccacagct ccacttccgt aactataaag
    31741 ggcgatttta tccccagctt tcaagctatc tgtattttcc aagagagaca aaagtccaag
    31801 gaaaagtgaa cctgtgtaga tattccccac cttttgactg tagagaatag actggtcaaa
    31861 atgcttttgt aagaggtctt ttttctcttg aggcaggctc ttatccatga tttttttcaa
    31921 gccttttagc gctaatttag gataaggcaa gtggaaacaa acagccgcaa aatcatccaa
    31981 agtaagctgg tagcgttttt gatattcaag ccaagtcgtt ttcaaactat ccaagtattg
    32041 ttgggtagaa tagacaccat ttacataagg agttgtcgag taatttggtc gccagaaatc
    32101 catgatgtca cgggtctgag ctacattgtc attattaaag gccatcatgc gtggattttg
    32161 tgtaatcaac atagccacac ttccagcacc ttgagttggt tctcccggag tttcaatacc
    32221 atatttggca atatcactgg caatgaccaa gaccttggac tccggagaat tttccacatg
    32281 caatttggca taatggaggg cagctgtcgc cccatagcag gcttctttaa tctcgaaact
    32341 acgagcaaag ggctggatgc ccagcaagcc atgcacaaag acggccgcag ccttactctg
    32401 gtcaattcct gactcggtag ccacaatgac catgtcaact tcttgtcttt cttgctcagt
    32461 taaaatagag tcactagcac tggccgccaa ggtcacgatg tcctcagtta ggggcgcaat
    32521 actcaattcc ttgagtaaga gtcctttact taatttttca gggtcaattc ccctcgcttc
    32581 tgctaagtct tgtaatttca agacatattg actggtcgca aaaccaatct tatcaatacc
    32641 gattgtcata tttacctctg ttttatcatt catgtaaaaa atcgttctat actattttat
    32701 cacaaatggc agtaaaagag agaaaaaaga cttgattcac caaatcaagc ctcttattgg
    32761 tcatcatttt aaagaatgat tagttgctag agagttcacc gatataagta gctttataag
    32821 ctccattcac agttatcagc tcctggagga tcaaatttcc tgagtaagtc cttcccatct
    32881 catctacaaa tttttgataa aactgactgg tcggaatttc tctgacatcc ttatcaaatg
    32941 tcttgtcaag tgttttacta accttctcag caatcaattg atgctcttgc catccacttt
    33001 gaaactctga gccccaacta gaaaccatga ctgggataaa caacaaggtc agtagactta
    33061 cagacaataa ggaaaatagt agacttcctg caaaactaga atcctagttc atgattgata
    33121 atgccagcaa tcaaattcat tcgtaatccg aagcgtttac gatgatttcg atagattgtt
    33181 gaaaacattt taaacgtttt tactttggca aagatgttct caaccttgct tctctccttg
    33241 gatagcgcat ggttacaagc tttatcttca gctgttagcg gcttgagttt gctggattta
    33301 cgtggagttt gtacttgagg atatatcttc atgagccctt gataaccact gtcagacaag
    33361 attttaccag cttgtccgat atttctgcga ctcattttga acaacttcat atcatgacaa
    33421 tagttcacag cgatatccaa agaaacaatt ctcccttgac ttgtgacaat cgcttgagcc
    33481 ttcatagcgt ggcatttctt tttaccagaa tcattcgcta attctttttt agggcgattg
    33541 atttgtactt ccgtcgcatc aatcattacc gtgtcctcag aactgagagg agttcttgaa
    33601 atcgtaaaac cactttgaac aagagttact tcaacccatt ggctccgacg gattaagttg
    33661 ctttcgtgga taccaaaatc agccgcaatt tcttcataag tgcggtattc tcgcacatat
    33721 agaaagcgtt gttaatttat ttatctcatt tttcagaaaa ttctttcatt tgtgtaaagt
    33781 ctacgatact cgatgtgttt ttatataatg atacagtctg agaatcactg ttccgctagc
    33841 cattccaata gagattacca aagccaacat gacaaccaag gtcgcacttg ccagtgcttt
    33901 attatagtcc cctgtcacaa aaaaggcagt tgttcggtag gagagataac ctggaaccag
    33961 cggtgccaaa atggccaaga taaagaccac agcaggtgtc ttataaagaa tacttaaaat
    34021 ctggctgaca caagaaccaa taatggctgc aatgaaggta gctacaatga cattggtcgg
    34081 ctccttgagc aagagataga ttagccagac agtcatgccc aaaatccctc caggtaagag
    34141 catagaccgt tgcacattga gtacgattaa aaaagtgata atggcaagaa aacttgctac
    34201 tgcttgtaat aaaaaggttg ttagtgtcat attagttcat caataccaag gcgacagaag
    34261 ttcctgcccc taaagcgagg gtaatgagca gggattcaaa catcttactc ataccagagt
    34321 ttatgtggtt ggtcataata tcacggaccg cattggtcaa ggcaatacct ggtacaaacg
    34381 gcatgaccgc accagctata atcaaatctg ccgttgaagg aaaacctgtg tagcgagccc
    34441 aaaactgggc aattatccca aagacaaagg ctccagcaaa ggctgtcaca aagggaattc
    34501 ggataaattt ttccacatag agggaaaagg caaaaccaaa taaggtcgcc actcctgccc
    34561 caagtgcgtc gtagatattt ccgctaaaca taactgaaaa gaaaggagcg ctaaaggtcg
    34621 cagccagagt tacctgcaac ttagtatagg gaaggggttg agcttgcaag gccgtcaatt
    34681 gcttaaaggc tgtttctaaa tcaatctgcc ccccaaccag ctgacgagaa atctggttca
    34741 catcgcagac tttttcgatg ttataagaag aggaggtcac gcgcttcatg cgcgaaatat
    34801 tggtattttc aatagagaaa aagatagcgg caggcatggc aaggacattg caatccacaa
    34861 tcccctgcga atgcgcgatt cgaatcatgg tatcttctac acgatggatt tctgagccac
    34921 ttttaaggag aatagtcccc gctagcataa tcacatcaat gacggtattt aattcttttg
    34981 attcttccat gctttcctcc ttttatcaac tccctctatt ctatcacaaa tccggactca
    35041 aaaaaaatct ttgccatgaa atcatgacaa agattgatta ctcattttga ttatccatct
    35101 gcttgattat ccatctgctt ttaaggagta gctgaagttg ttttaggttt gtagattgaa
    35161 atcttgactc tagtcttatt gaggtctacc ttttcacctg ctctaggact ttgttcaaca
    35221 accatgcctt ctgcactacc tgcaggcgct gtcgtcactt ctacaacttc tatattagct
    35281 tccttaatcc caacaatttg aatcaaattg ttcttagtaa actccaagct ggaaccaatg
    35341 taactcggca tggcaacact tgtaactttt ttagctactg tcaagacaat ttgaataggt
    35401 ttactcacat cataggtcgt tccggcacct ggactttgtt tcataatcgt tcctggttcg
    35461 ctttcgctgg actcttcttc ctctatctta atcaaattct caggaacctt cttctgcttg
    35521 agttctgaga ttacttctgt agagttccgt ccaatatagt tccctaattg aatcgtcgta
    35581 gcttttttag ctactgtcaa aacaatttga gttgccttgc tcaagtcata ggtcgtacct
    35641 tctggtagac tttgcttcag gaccgttcca gcctcactct cattggactc ttcttcctca
    35701 attttaatca aattatctgg aacttttttc tcttttaatt ccgcaatgac atcagaggat
    35761 ttccgaccga cataattact aatttggaag gattgcttgc ctgatgagac aaccaaattg
    35821 attttcgttc cttcttttcg accagttcca gcgccaggat ctgtacggat aatccgccct
    35881 tcttccacct tttcactagc ctctgtcttc tcctcaccaa tctcaaaatt ggcttttttg
    35941 agcgttgcct tggcctctgc aactgtctga cctgccacat ctggaatggc aatggttgca
    36001 ggagttctgg atagtatcca aataagagaa gctgccacca atacaaggct ggccaacaaa
    36061 atcaggtaac gcatcttaaa tctatgtttt ttcggtgctt gtggttggta agtttcctct
    36121 gtcacagcct ggcttggggt tttgattgat ttgtgttctg tctgcgcttg aaccttagga
    36181 atagatgtca aggtactctg agaaaccttc ggcaaggtct tggtatctgc cttgctcgtt
    36241 tcatcaaaga ttaacttact ttcatttcta cgattgtagg acaagctact agacaagtcc
    36301 acatacatct ctgaaaccga gcggtagcga ttggtcaact ttttagcagt tgccttgata
    36361 ataacatttt ctaaagcctg aggtacagat ggattttctg caataacgga cggcaggggt
    36421 ttctggaaat gctggagggc aatggtcacc gcgctatccc cgtcataagg gatatggcct
    36481 gtcaacatct catagaaaat aatccccata gcatagatat cactctgcac agtcgccttc
    36541 gaaccacgcg cctgctctgg ggacaagtaa tgaactgagc ccaacatcga gttagtctgg
    36601 gtcagacttg tctctgcaaa ggctacagca atcccaaagt ctgtgacctt tgcagtccca
    36661 tctggtgtca aaaggatatt ttgaggtttc aagtccctgt gaacaattcc tctggtatgg
    36721 gccaaacgca tggctaggag gatttgtccc atgatacgga ctgcttcttc attagaaaga
    36781 ggataatgtt ccttgatata gcgtttgagg tctagtccag caacatactc cattgcaaga
    36841 tactgttgac cgtcttcctc accaatatct gttatccgaa cgatatgagg atggtctaga
    36901 tctgccatag ctctcgcttc acgctgaaaa cgagctacag ctatcgggtc cgtctggtag
    36961 ttggtcctca gaaccttcac tgccacttct tccccatcta agattaagtc tttggctagg
    37021 tagacatccg ccatacctcc tcgaccaatc tgtttgacaa tccgatagcg tccggcaaaa
    37081 atcttgccga tttggatcat tctgcatcct cctcgttcat agaaacaagg gcaaccgtaa
    37141 tgttgtctaa acctcctgca ttgttagcaa aacgaacaag tgtctccgtt ttatctgcta
    37201 aaggaatatc actggttaca atatcacgaa tctcactgcc tgaaatcatg ttggtcaagc
    37261 cgtcactatt gagcaagaga tagtcacctg actcaaggat aactgtccca aaatcaggct
    37321 gaatttcatc tttttgccca atagactggg tgataatatt tttttgcgga tgagcttctg
    37381 cctcttctgg tgtcaattga ccagccttga gcaattcatt aaccaaggaa tgatcgctcg
    37441 tcaactgatg gtattcttct ccacgaatca agccgatacg cgaatcacca atatgagcat
    37501 agatagcctg attatcaata atagcaagga cttccaaagt agttcccatg cctctgtaag
    37561 cttcatcctg accaagctgg tgaatctttt gattttcaat ttctaggtaa tgggcgaacc
    37621 attcacgcac ttcattgact gtatcgatct gggtatcaac ccaagctaca cccaggtctg
    37681 tgaccgccat ttcactagcg atattccctg cacgatgacc tcccatccca tcagctaaaa
    37741 taatcatggt acgtccagct ctattgacat agtggttgac atagtcttgg ttatttgttc
    37801 gtttctgacc aacatctgtt aataatgaaa tttccatgtg tcagttcctt cctaatccga
    37861 tatcttgcga aattgactga tgaagaatcc atcacttcca tacaattcag gtgtaatgag
    37921 gatacagccg tctttcatga tatccttaca ttcatgttct agttttacct gctcgaactc
    37981 gggatgactc tctaaaaagg ccttaacgac ttgaaaattc tcctctgaga cgatagtgca
    38041 ggtgctataa gttattatac cacctttgcc tagtatttga caaacactac ctaatatttc
    38101 taactgaatt tcctgcaagg acgcgaaatc tgccgtttct ttattgtatt tgatatctgg
    38161 ttttcggcgc aaaagaccga ttcctgaaca aggagcatcc accaaaatct tatcaaagga
    38221 atcctggtca aaaaactcat gcacctttct ggcatccaat ttttgagttt gaacccgatc
    38281 ttcaactccc agacgttggg cattttcttg aattaaatcc aacttgtggt cgtacaagtc
    38341 cagagcagta acctgacctg tcgtaagata agaggctata tgggctgttt tcccacctgg
    38401 cgccgcacag gcatcaagca ctcgctcatc accttgtaaa tcaagcgtcg gagcaaccag
    38461 ctgactggac tcgtcttgga tggtaatggc tccatccgca aacaaattat gccctgcaaa
    38521 atgcccctgc tccttaacca gaccagtggt tgctaaaagg gaatcattcg cctccaacaa
    38581 ggcttggatt tcctcttttc gacttaggtc tgttacacga atactggctt tgtttcgcac
    38641 taacaggctt tcaaagatgg cttttgctct ctcctctccg tattcttcct tgagtttggc
    38701 aactagccaa actgggagag aataggcaat ggagtcacgc ttgttttttc gcttgatgct
    38761 agcaatatct ggccagcctt cacgcaagat acggcgaagg acagcgttga ccaatttttc
    38821 actgcctttt ttacggagtt tggctaattc cactgcttca ttgaccacag catgatctgg
    38881 aattttgtcc aaatagcgga gttggtaggc actcatgaga agaaggacat agagccagct
    38941 gtctaactgg tctctgtctt cgataaagtg ggataggtac cattccagag tcagtttacg
    39001 ggctaccgtt ccatagacta gctcggtcac taagcccttg tctgctgcag agagctgact
    39061 ccccttgagg tgtttattta aggcaatatt tgaatatgct tgattcacaa aaacatcctc
    39121 tagcactgct agagctaaac ttctagccgt ttctacttta gtcaccaaat cgttctccta
    39181 cagtcaatgt acgtccaact ccattgagga aggaagcaat gtccatctta ggcttaccag
    39241 ctggctgcac ttgtttgagg gatagagccc cttcagccgt tgcgacaatc aattctttct
    39301 tgccgataga gagaatctca cctggatttc cctgaccttc tactggtagg gcttcataaa
    39361 tcttaaagcg gtcgccctta aggaaagtat gggcaacagg ccaggggttc attccacgaa
    39421 tttggttaaa gagttgacga ttggttttgt tccagtccag tttttcttcc tttggcttta
    39481 tatttggaga gaaggtaacc tgactcgtat cctgcggttc aggtttgata tcaccagcaa
    39541 tataggcagg cagagtgtcc aaaagcaaat cacaaccaac tagcgccaat ttttcaaaca
    39601 aggtgccaac attgtcctca tctgtgatcg gaatgctgcg acgagaaatc atatctcctg
    39661 catccatttc cttaaccatt tccatgatgg tcacaccagc ttcctcatcc ccttgaatca
    39721 aggcataatg gataggcgca ccaccacggt gtctaggaag gagggaggca tgaacgttga
    39781 cagcaaagtc catgctatca aggagtttgc ttgggagaaa ctgcccaaaa gcagcagtca
    39841 caattccatc tgctcctagc ttcataagat cttccatctc tggacttcca gataattttt
    39901 caggttggta gatagatagt cctgcttcct tggcagcctg cttgactggg gtttcttgga
    39961 taactttttt acgaccaaca gcacggtctg gctgggtcac aacggctaga atttcgtaac
    40021 ggtcatctgt caaaagtcct tttaagactg ttgctgaaaa gtcgggggtc cccataaaga
    40081 ttagttttgt catatcttct ccttcttata aaaattgctg cggctcatgg tcaatgctga
    40141 gacggagctc actattttcc cgttcttgag tcaaggccaa gacctggttg agggtcggcc
    40201 ccagctcatc ttctaaacgg tatttaatta aaatctggta atgatagagg ttgtgggtac
    40261 gggcaatcgg ttttggcgtt ggccccagaa tgggactggt ctctgacaag cctgaccgca
    40321 aaatgttcat gacttcatag gcacgtttga aaacctcttc ttctttcttg tgagaaaggg
    40381 taataccaat cgtgaaatag taaggtggat agccgagttg tcgtctgatt cccatttcat
    40441 aggcataaaa gccttcgtaa tcttgatcct tggcaaatcg aatagcatag tgctgcgaat
    40501 tgtaggactg tatcaagact tgacctgctt tttcagcccg acctgctcga cctgccacct
    40561 gagtcaagag ctggaaggtt ctctcagaag aacggaaatc aggcagattc aaggccgtat
    40621 ccgcatttag aactccgact agggtaacat tgggaaaatc caaacccttt gcaatcatct
    40681 gagtaccaag taaaatatcc gcttcccctc gcccaaactg gtcaagcaag gcttggtgac
    40741 tgcctttctt tcgagtcgta tccacatcca tcctcaaaat gcgagcttgg ggaaagagtt
    40801 ctgctagctc atcataagcc ttctgagttc ccgtcccata gtaacgaata ctgcggctct
    40861 tacagttagg acagacctga ggaatatcct tcgagaaacc acaataatgg cagttcatag
    40921 tcttggtatc catatgcaag gtcagagaaa tatcgcagtt gggacaagta tccaccgtcc
    40981 cacactcccg acacatgaca aagctagaat aaccacggcg attgagcatg agaaccacct
    41041 gctctttttt aaccagacgg tcttggatag cctctagcaa aggaggcgta aagtttgacg
    41101 tctcattttg tccgatatag tctcgaaagt caatcacttg aacctcaggg attgtagcca
    41161 aaggattggc acgttgggtt agacgtaagt gtcgatagac gcctttgcca gcacgtgccc
    41221 ggctctctaa gctcggcgtt gcagatccaa gtaccagagt tgcttgatta tactgagccc
    41281 gtaaaatagc tacctctctg gcatggtaac ggggattgct gtcctgctta taagccgctt
    41341 catgctcttc atcaataatc atgacaccca gatttttcag aggagcaaag atagcagatc
    41401 tggcaccaac aacaacttgg gcatcgccac gctccacctt acgccattca tcatactttt
    41461 cgccattgga taatcctgag tgaagaatgg ctaccttgtc cccaaaacgt gctataaaac
    41521 gctcggtcat ctgaggagtc aaggaaatct caggtaccag caaaatagct gtcttaccct
    41581 tatccagggc accttggata atctgcaagt aaacctcggt cttcccactt cctgtaatcc
    41641 cttgaagtag aaagggaggt tgagaactgc caatagaact cacaaccgca tcacgcgcct
    41701 gtctttgttc tggatttaac tccaaaggtc tacttgcttc aattccttca aaataagcag
    41761 ccgagcgttg aacttccttt tggactatgg taacagcacc ttgatccaca aagaagttga
    41821 cttgctctcg cgagtaggac tctaacaagc tagccaagga agcgctctct ggatgagaca
    41881 gcagataatc tctcagttcc aactttttct tggcacgtgt agaaatctca acaccttcta
    41941 attgggcatg gtcaacctca taccaagact gggtcttgac cttcttttga tcgactgcct
    42001 gatattccag accaagcagg ccttttctag tcaaacgcat catttcagct tgcttggcaa
    42061 ggtctagtga agaaaaggct agcgaatctt ctgaaccaaa caggcgtact cgttcttcct
    42121 gactcaagcc ttccagagga tagagaatct tgtcatagct agaattcaga aaccctggaa
    42181 gcatggcctt gaggatagag attttgtagg agaagacaga tttgcgtaac tcctcagcca
    42241 gccagagttg ttctggcgtg agaacaggag aaaaatccag cacctctgca atatctttta
    42301 aatcttgctc catctcttct ccatctgatt gggacttcaa accaagaaca atcccttgaa
    42361 tcaggcgatt ccccttacca aaaggcacat gaacccgcat cccaacttcc agcattccct
    42421 caaattcctc cggaatcctg taactatagg gctggtccgt ctgcatcaag ggcacatcta
    42481 cgataatctt agctagggcc atcttctcac ctcctccttg tcagtacatt cttgcaatag
    42541 aaaaaataag attgagtccc cccaacctta aattttttca ccatcttctt tttctttagc
    42601 aatttgctct ttgattttct tttcttcttc ttctttgcgg cgtttttctt cttcgatacg
    42661 gcgacgcact gcttcacgtt ttccttctgg atctgggtga attgtaacgt ttcctgattc
    42721 gatttcttct aaagcgcgaa gagttgattt ttcagacttg aaaccttgag ttgctggggc
    42781 acctgcttcc aattcgtggg cacgttttgc ttccaagatt acgagtgaat attttgaagg
    42841 aaccttgtcg agcaaggtat caatagaggg ttttaacatc atttgcttgt acctattttc
    42901 taaattttat cgggtagttg gagattttgg taacatctcc tgatagtgac caatgacacg
    42961 atccacacag aagtgttctg cttcaatcac acatttgaca cgttcagcag ctaggggcac
    43021 ctgatcgttg acaatcgcat aatcatactc acgcatgagg gcaatttctt ccttggcctt
    43081 ttcgattcgt tgggcaatca cttctgcact atctgttcca cgacctacca agcgatcttg
    43141 caattcatcc aaatctggtg gtgtcaggaa gataaagaca gcatctggaa cctttttctt
    43201 gacctgaaga gcaccctgaa cttcaatttc aaggaaaaca tcgattccct tgtccaaggt
    43261 ttcattgaca taggtcagag gagttccata gtagttgccg acatattctg cgtattccaa
    43321 catctgtcct tgacgaatca gctcttcaaa ttcttcacga gtacggaaga aatagtcaac
    43381 accgtccact tctccaggac gttgtgcgcg tgtcgtcatc gatacagaat attgaaattg
    43441 gttttcagaa ctctcaaaaa tctctcttct aaccgttcct tttccaaccc ctgaaggacc
    43501 agaaaaaacg attagtaagc ctcggtctgc cattgtgtct ccttttagtc aatctgtgaa
    43561 ataacatttc tctagaataa tggcaaaaag ccagattatc ctttacagtc tttctatcta
    43621 gtgtaacaaa aaagcagtaa tttttcaact gctctttctt atttatttag cataatctac
    43681 tgcacgaagc tcgcgaatca cggttacctt gatatttcct ggataatcga gattgttttc
    43741 aattttctta cgaactttgt gagccaagat tgtgactttg tcgtccttga tttttcctgg
    43801 attgaccatg atacgaattt cacgtcctgc ttgaagggca aagctagttt gcactccttc
    43861 aaagccgtta gcaatttctt ccaaatcatg gagacgcttg atgtagcttt caagagactc
    43921 actacgagca cctggacggg ctgcgctcaa ggcatctgct gcagcgacga taactgctat
    43981 cacgctctca gcttcaacat ctccgtggtg actagcaatc gtattcacca caactggggg
    44041 ttccttgtac ttacgggcca attccatacc gatttcaacg tggctacctt caacctcatg
    44101 gtcaatggct ttcccgatat cgtgaaggaa tccagcacga cgggcaagag ccgcattttc
    44161 accaagttcg ctcgccatga taccagccaa cttagcaacc tcaatcgaat ggcgcaaaac
    44221 attttgtcca tatgaagtac ggaactgcaa acgtcccata atcttcatca agtctggatg
    44281 aaggtttggc gcaccaattt cataggcagc agcctcaccg tattcacgaa tcttattgtc
    44341 aatctcttga cggtttttct caaccaactc ttcgatacga gctggatgta tacgaccatc
    44401 tttgagcaac atttccatag tcatacgggc aatctcacga cgaatcggat caaatcctga
    44461 caaggtcacc acttctggtg tatcgtcgat aatcacatcg acccctgtca aactttcaaa
    44521 ggtacgaatg ttacgacctt cacgaccaat aatgcgtccc ttcatagtat cgtctggcag
    44581 atgaactgtt gagtttgttg actccgctac atattcacca gcgatacgtt gcatagcttg
    44641 aaccaagatg tccttggcca ttttgtcaga acgttccttg acctcttgct cagcttcgcg
    44701 aatgcgactg gcaatctccc tggtcaagtt ttcctctgtc tgagccaaga taatatctcg
    44761 tgcttctgcc tgagacagag caccaatacg ctctagttct gcttcttttt gtctttcgac
    44821 ttcctctaat tgctcttcac gcgcatcaag gtttttcgct ctatcagaaa tactttgttc
    44881 tttttgttca agtgtttgtt ctttactcgt caaattgtcg tccttacggt caaggctagt
    44941 agctctctct gtcaaacgac tttcgatttg tttgagttct tgacgttctg atttgaattc
    45001 agcgtccact tcttcacggt attttctggc ttcttctttg gcctccaata gtgcttcttt
    45061 tttaagagac ttgctttcac gtttggcttc attaacaagt aaatccgctt cacgctcagc
    45121 ttgtccacgt aaattagttg cttcttgttc agcatttaaa agcatcaact ctgcagcttc
    45181 ctgagatgat ttcatcttag ctgagatgct gacatatcca atgactaaac caatgatgac
    45241 ggcaaaaaca gcaatcgcaa gcgacatgat ttccatgttt ttacctcatt ttattgttat
    45301 tccgaatgac atacattctt ttacattcta ccataaaaaa gtgattttca caaacctaaa
    45361 atagaatatg ttttggggaa tttggaacac atttaccaaa ataaacttgt tgtttagaaa
    45421 tagtagttta gtagagactt gagaaaaagc ctactttgca gtagacttcc taatgattat
    45481 gcaatacaag aagtttgttt ctaacaacag caagatttca ctcaggtatt ctctgtaaat
    45541 tagactagtg tcctgcgcga attttatcca ttcctcgcaa aaccttgtct gataactcct
    45601 tattttgggc gattctcagg gtttcctgaa tactgtccca ttcgctcttt gaaagaacta
    45661 caatgtcctc atctggattt ttattgacca ccgtcaaagg ctcaaattca tcatttacct
    45721 tcttcatgta atcctttaaa tgatttcgga atgttgagta aagaactcct tccataacca
    45781 tacctcgttt tagctctttt ccactattat acacgaaaag aaagaaattg tcaggaacct
    45841 gtacaagatt ttcttttcta tctatttata ctcaatgaaa atcaaagagc aaactaggaa
    45901 actagcctca ggctgtactt gagtacggca aagcgacgtt gacgcgattt gaatttgatt
    45961 ttcgaagagt attattcgta aaaaatctca aaaagcctac cttttagtag acttagtttg
    46021 tttctattct aatcggcact cttccaaaat tttgctctgc tatacttggc tttcctagtt
    46081 ggtaaatctg gtctgctttt tgggtcatag catcccaagg ttctttgcca attcggctga
    46141 ctagattgac ctgtcctttc agagacttga gatgttgcct gcctttttca gtaaatccaa
    46201 ggacatgaat ggcttctggc aagacatttt ccctagcctg caccaaaata taggtcaata
    46261 ggcgtctgac acgcgccttg gtgtaacgtt tggtagtcac taactcgacc aattcttcca
    46321 cagactgggc tgttttaata gcttctttaa tgcgcacagc catttcttga ttgacctgat
    46381 agatggtggt taggtcggga tttgacaaga tttgatagcg gagcaaggga aaatagtctt
    46441 cccagatcac cttactggcc tgctcaaata gggcaacaga aggcataaaa cgttctaaga
    46501 aatcttggtc cttctgatgt tgacggaggg ctgtcgccga ggcaaagtcc acatctttat
    46561 tcacagaatg gtaacctgcc ccctgacgct gaatcggatg cagcttgata tttcgtcctg
    46621 caaccgcctt tgcataggcc agagcaagaa catgattggg ggtattacct gaaaaatcaa
    46681 gaccagcaaa ttccttccac atagattggg ttttctgggg ataggaaagg gaatcaggca
    46741 gattttccac aaatttctcc atctcagcac ctttctctgt gtataagtca gcgattttct
    46801 gataatctcg aacttcctct gtcccaaaaa ctagactatc aatgcccaag cgatccaaga
    46861 tatccacagc tccttgacca aagaaatcag ctgcctgaac actgactaaa aagggcaatt
    46921 ccactaccaa gtctgcgcca ttttccagcg ccatctgggc ccttgtccac ttatccacaa
    46981 tagcaggctc tccacgctgc atgaaattcc ctgacatggc aacaattttc agtccctctg
    47041 cctgatccag caggtattta tggccattat gaaaaggatt gaactccgcg ataatacctg
    47101 tgatggtcat gctaatctcc tacttctgcg ccacaaaaaa ccaacgggtg ctagtttctg
    47161 ttggttcctt atcctcaaag tctgcataga gtttgaagga cttgaatcca gcctgttcca
    47221 gtaagatatc ataggtcaag atctcatagg tcctctcctc atgtacttca tcgtggcgac
    47281 taaaggaacc gtcagcctcc ttgataaaga aagtcagctc atgcacgatg gagtgaggag
    47341 cttcgtcctc atatgtatcc caaagcatgg caaaatcttc cgcattttca tggtaggaat
    47401 agccagggaa tacttcatct gtctggtagg tcgagtgcac gtcaaagatg aaaactccct
    47461 cttcattcag cgcattgtac acgtccttaa agacgtcccc tacttccacc tcatcctgca
    47521 tatagcagat agagtccgaa taacacgtga caaaatcgta tttccctgcc ttggacaaat
    47581 tcagcatatt accttctata aaagcaatct tttgcttggc tgaagttgct ctcttctccg
    47641 caatcttcaa catatccgca ctcaagtcaa gtccagtcac atcaaaacca gcctgagaga
    47701 agcgcactga ttgaattcct gtcccacaag ccagttccaa gagtttcttt ttctccttgg
    47761 tcttaggcaa atgacgcaga gaaaaatttg tccatttgtc gtataaacta tcgtccatga
    47821 ccgcatcata gaccgccgca aaggtttcat aagtcgccat aatcatgata ctaagagctc
    47881 catggtaaac accactagcc ctttaccttt ctatcaattt ttctaaaata agcaaagaaa
    47941 cccagttgac tacaactgag ttcatcttct aagcaagagc ttctgaaata tctactgaat
    48001 tcgcctcatg ccatagcttc tctagattat aatgggcacg catttcttct gaaaagatat
    48061 gcacgacaac agcaccgagg tccagtaaga cccagcctcc agctgcatcg ccttcgacat
    48121 ggctaccttt aaagcccgct tgagctactt tttcacggat attagctgcg atagcgtcca
    48181 actgacggct attcattgag ctagtgatga caaagtagtc cgtcacacta gtcaaatctt
    48241 gtacatcaag tgcgaggata tcctccgcac gtttctcatc agccgctttc acgactagtt
    48301 ctagtaattc tttttcgttc atttaatcct ctttttagaa aatatgtttg taatctagga
    48361 catctgcaaa gcgctcctcc caaatgtcct cataaaattc attaatcgtt tctgctggaa
    48421 tgtcatcccc atcaaaggtt gtgacagcac cttctggaac aataagctga tagccatatt
    48481 caaaggcaac cttgacagag gtatccacac aatattctgt ctgcatacca cataaaacta
    48541 atttttcaat cccctgctta tccaagtatt cttttaagcc agtttctttg aaaatactgt
    48601 tatacttctt ctgaaagacc ttttcagcag gttttcgatt taaaagcgca gataactgcc
    48661 aatcttctga tgtttgagct tcagagttct caatatgttg aacatagata atttcaatat
    48721 tcttgcttct agcctggttt tgtaaataag aaatttttcc caatagactt tttgtctgga
    48781 acccagtttc tactaaaaca ttctgaacat caatgattat aaaagccgtc ttcatttagt
    48841 tctctttcaa atagtgcaca aaggcgttat aggtttcaag ggtttgggga tagatgggga
    48901 atccctgatg agctagatac tccacggtac gagctgtttc gtaggccacc gccttattga
    48961 gcgatagact tgcaatctca cgcgccacat ctactccagg aaaggctcga ttgtgctcga
    49021 tatagtctgc gacgtagatg accttatcta ggtctgtcat ttgaccagca ccgactgtat
    49081 ggacttcaat ggctcgcagg atttctgaat catgcaaatc caaatcttcc tgaattttgt
    49141 aaattccgac cataccatgc cagacattat tgccccagtt tttgaggtca gggtctagct
    49201 ggtaacggtc aatcaaggct agaaattcct gatctgacaa ctttttagca tagtcatgaa
    49261 gaagacctgc tagacttgct ttctcaacat ctacaccaaa gcgctgagct aattccatag
    49321 cggcacgctc cacacctaga caatgggtta gacgtttctc aggtagaagc tctgccattt
    49381 tttccaacaa ggcctcacgg gagcagttga tatagtcttg ataggacatc agtagagccc
    49441 ctccttctcg atgtagtcta gcactggctg aggtaggaga aagttgggtt tccgaccttg
    49501 ggcaaggaag tcccgcacca tgctggacga gatatccatg agcggtacgt ccacccagat
    49561 aactggatag gaagtcccta ccttgtagcg tggacgctga acccccacaa actgaaccat
    49621 gtcaaccagt tcatcaattc ggtaccactt aggcagatag gcaaccatgt cggcaccgat
    49681 gataaagtaa taatccgtat ctggattctt ctctgtcaaa atcttcatgg tatcgtaggt
    49741 gtaggaaata cccttgcgct ccaactcaat ggtttcaatg actaggccgt caatcccctc
    49801 aattgccaac tcaagcatct tgagacgatg gtgttcaggg atggtttcct ttttatcaac
    49861 gtgaggaggt tggtattcag gcatgagaag aacttgatcc agtcccaact gttgccgtac
    49921 ttgatccgca acaatgagat gggcattgtg aacagggtta aaattccccc ctaaaatccc
    49981 aacttgtttg cgttttttct ccttgatttc tggctccaac tctaccttgg taaagggagt
    50041 caatagttcg attgtcatag gctagtcccc tttatttctc tataaaaaca gttatttgga
    50101 gtttcggtct tagatttctt tgactttctt agaaatcttg cgattttctt tcttgctaga
    50161 ttgtttaaac aaaatcaaga tgcgtcctat tttttggact gtatccacac cgatttcttc
    50221 ttccaaaatt tcagctactt cgtggatgtt ttcatctgtg ttttgtaaga gagtaacctt
    50281 gattaattca cgcgcatcaa gagcttgacg gacgctggtt ttgatttggt cgttgagtcc
    50341 atttttcccg atttggatga taggtttgag ggtgtgtgcc tggctgttga ggaaggcacg
    50401 ttgttttgat gttaatgaca taattttctt ccttgttttt ggatagttat tttaggtggt
    50461 gagggacggt cggaactaat tttccaaaga tttcatcttt gaaaaatgga tttcctgacc
    50521 tcacaattga gccttggtct caattgtgcc ccttgccaat ctacagattg gcaatcacac
    50581 cacggcaata actatcgcta tatgagctca ctttgttcgc tcagcgattt tgttttaaat
    50641 aattgctttt cgtgtgacga cggcgacgcc ttctggtgcc cagacggcga cttttgctat
    50701 gcctgttaca cgaatccagc ctaggcctga gatgactagg tctgtcttat ctttaatggt
    50761 aaagacatgc tggactagac ttgggaaatc ttctttttcc ttgctatttg gtggtgtcag
    50821 aagagttccc aggtgcttat cgtagaaagc actagctcct tcaagcttgc tacgatggag
    50881 tttgagttca ttatcaaaga aagcagtaaa tccttgcttt tctcctgcta taaagtcaaa
    50941 gcgtcccaaa ccacctaaaa atagggtttg ctcaggatta agctgatagg tcttaggctt
    51001 gatttccttt ttaggactga catacttgag gtttttggcc gtcaagtagt gagccatctg
    51061 gtggcggtgg ataattcccg gcgtatcgta aatataagat ccgtcgtcaa gcggaatctc
    51121 tattttgtcc aaggttgtcc ctgggaagcg tgaagtagtg atgacattct gatcacccgt
    51181 gatttcttgg ataatagcat tgattagagt tgattttcca acgttggtca caccgaccac
    51241 atagacatcg cggcccttac ggtagtgttc aatcttgtca atgacttcct taatggcatg
    51301 tttattttgt gctgaagtta ggaccacatc gactggacga agaccttctt catgggcacg
    51361 tttcatgagc cactggctaa tcttaccaga cttaactgac ttaggaagga tatctttttt
    51421 atttcctacc aagaggacat cattgcccga gacgaaacgt ggtaaacctg ggatgacaga
    51481 tccattaaaa tcaaagatat caataacatt gaccactaaa gcatcactgt ctcccacctc
    51541 gtgcaagagc ttgaggaaat catcgttcgt caactggaca tctgtgattt cattgtagtg
    51601 gcggagacgg aaacagcgtt ggcaatagac ttcgccagtc tccaaacctt tttcaagtgc
    51661 cgactggggg gtaaaaccaa gaccagcttt atctgtcgtc tgaatggttg ctccacaacc
    51721 aatacagaga atttcttcca tagttaaatt ccttttttat atgtaatcgg tccgtacttt
    51781 tcagtgattt ttctcataac acgacgctca cgagttcggt taatctgcgt tttgattgag
    51841 tcatgttgga ccaagggttt gactaaaatt gaccgaatcc ctgcacggtg ggctgctcgt
    51901 atatctgtca tgagctggtc accaaccatg accacttcct ttttgtcata gtggaattcc
    51961 ttcatagcac ggtcaatacc aaatgtgaag ggcttcaagg cccagtaaac gtaatcaatc
    52021 ccaaatttct caactgctcg ttgaacgcgt tttttggtgt tatttgacac tacgataatg
    52081 ccaatacccg cgtcccgaag gtcatgtagc cattgcttca tctctggcgt tccatcaggg
    52141 ttgttccaag caatgagggt attatccaaa tcgaccaaaa cagccttgat tccctgcgcc
    52201 tgcaggcttg ggactgtcag atcatagact gcttccacag caaaatctgg tatataattt
    52261 tcaatcgcca ttctggctcc ttttcttaac tttttttcgc aattttcctt agtaacaagg
    52321 ataaaacaat ccacaaacct gcactagcca aactgatata gagccaagca tggggctcat
    52381 ctgttaaagg taagggaaca ttcattccga aaaagcctgt cacgactgcc aaaacagcta
    52441 gcaagactga aatgatagtc aaggttgtca aattgtcatt cagattattg tttagaatat
    52501 tgttgtaaga ggctgaaagc tgctgtaaaa tctgagagat taggtctgtc atggatacca
    52561 gctgatgagc ctcaatcatg gcatcatcaa actgttctct ctcaatctca tcaaaactac
    52621 gatacaaggc atgaccttga atatgctcta acaaaatccg attttgtttg gcagctgccg
    52681 tcagataaac cataccagtc tccaaatcag acagggcaaa gaggtttttc ttggtagttc
    52741 gctggcgcaa gaggtcattg acctcatccc tactcttgtc catctgctca atgacaggat
    52801 agtaggcatt gctgataatt tccagactgg caaagagaaa cttataaatc gaaagcgtgt
    52861 catggctctc cagataacga gtcatctgtt caatgacata ggcgttcttg gtattactaa
    52921 tggtaatcag gcgacgatgc tcgacaataa aggtcatggg aaaagtctca tagtaggcct
    52981 tgtccttttt tacgtctagg acattataga taaaggtaac cgtcccactt tcacggtggt
    53041 agtccatgtg ggcgcgctcg tttctatcca gtgcgtattc aatggtttcc ttgtccaaac
    53101 cgtaaatttc agaaaggtct tctagtttat tcaactttcc taggtctagg tctatccagg
    53161 tacaaccatt gcccaactgc ttttctaaaa ccatcttttc tccttattaa actccctcta
    53221 ttgtaccaca aattacttaa aataacaggt ctagtctgtg cgtgagaaat tgctaacaac
    53281 tgtgatattt cggttaggga caaagtgaag gacttgctcc tcgctacgaa gctgtgtcgt
    53341 acgaattccc acactgacaa ccttacccga tacagtaatc ggtccatttg tcagaacgac
    53401 ctcatctccc acatccagtt gacgttcaaa gaggatgaaa aagccattga tgacatcaga
    53461 cagaaagcct tgggctccca taccaatcgc taccccagca ataccagctc cagccagcaa
    53521 actagaaact ggcaaaccta aaatcgacaa aatgcagtag agtaaaaaga aatatagcgt
    53581 ataattaaac acattttcta gtaaacgtga gatggttttt tggcgtccaa catcatgacg
    53641 agacattttt agagaaggtt tgacaattct ctgcaccatg gtatgaagca tttttttagc
    53701 aatataaaat acaatcaaaa gcagtaaaag agaaatgacc ttggttagaa tattctcgat
    53761 aatggttgtc acatctagct tttcaatata agcctgaata aatttttgca tataaaactc
    53821 ctttatttca ttataccata ttttcatcag tctcattctc cataaatatt caaaaaatag
    53881 acagaaaatt cttgattttg tctatcattt atactataat cataatcact acacaaaaag
    53941 gagattatta tgaaaagaat cattcatgct tggaataagg caagccttgt caagcgtatc
    54001 ttgattggta tgctttgtgg ggaatcctag gactgaccct tcctaatctc tcagggattg
    54061 ggctactcgg ggatttattc gttggaggtc taaaagctgt tgctcctatc ctagtctttg
    54121 ccctcgttgc caatgccctt tcccaacatc aaaagggaca agatagcaat atgaaaactg
    54181 ttgttttctt gtatatccta tgatagggac tttcgccgct gctcttgtag ctgtactagc
    54241 aagtttcatc gtccctattg aaattaccct aaatagtgcc aatactgaaa ttgcaccacc
    54301 agatggggtt gggcagatcc tcagcaacct cttgctcaaa ctggttgaca gcccagtcaa
    54361 cgcgccctgt ttactgctaa ctatattgga atcttatctt gggcagtcat ttttggaatc
    54421 gctatgagag aagccagtaa aaatagtaaa gaattgctaa aaactatcgc tgacgtgact
    54481 tctaaaattg tcgaatggat catcaatctg gctccatttg gaatccttgg tcttgttttt
    54541 aaaaccattt ctgacaaggg agtcggaagc cttgccaact acggtatttt attggttcca
    54601 ttagtaacga ctatgctttt tgttgcccct gtggtcaacc ctttgattgc cttcttcttt
    54661 atgagacgca atccttactc tctagtttgg aactgcctcc gtgtcagcgg tgtgacagcc
    54721 tttttcactc gtagttctgc gactaacatt cctgtcaaca tgaaactctg ccatgacctt
    54781 ggactcaacc cagataccta ttctgtttct atcccactcg gttctactat caatatggct
    54841 ggagtagcga ttaccattaa ccttttgacc cttgctgcag ttaacactct tggaattcct
    54901 gttgactttg ccacagcctt tgtcctcagt gtggtagcag ctatctcagc ctgtggtgct
    54961 tcaggtattg ccggaggttc cctccttctt atcccagttg cttgtagcct tttcggtatt
    55021 tctaacgata ttgccataca agttgttggg gttggttttg tgattggtgt catccaagac
    55081 tcatgtgaaa cagcccttaa ttcttctaca gatgtcctct ttaccgccgt tgccgaatac
    55141 gcagcaaccc gtaaaaaata actcatcaag gcaagcctgc tcatgtcttg tcttttacgc
    55201 ttttattcta acttattagg aaattcttat gtctattagc caacgtacga ccaagctcat
    55261 cttagctacc tgtcttgcct gcctgcttgc ttattttctc aatctttcgt cagcagtttc
    55321 ggctggaatt atcgctctct tgagcctatc tgatacgcgt agaagtactt taaaactggc
    55381 tcgcaatcgt cttttttcta tgcttctagc tctggctatc ggtgttctag cttttcactt
    55441 gagcggattt catatctgga gtctcggcct ctatctggcc ttctacgttc ctttagccta
    55501 caagatgggc tgggaaattg gcatcacacc aagcacggtt ttggttagcc atctcttggt
    55561 tcaagagtca acctctccag acctcctagt caacgaattc cttctctttg ctattggtac
    55621 aggatttgcc ttgcttgtta atctctatat gccttcacga gaagaggaaa tccagcacta
    55681 ccacacgctg gtggaagaaa agttaaaaga tatcctccag cgcttcaaat actatttatc
    55741 cagaggagac ggacgcaacc gagcacagct ggtagcagaa ttagacacgc ttttgaaaga
    55801 agccctcaga ctggtctatt tggatcactc tgaccacctc tttcaccaga ccgactacca
    55861 tatccactac tttgagatga gacagcgaca aagtcgtatc ctgagaaaca tggcccaaca
    55921 gattaacact tgtcaccttg ccgccagtga aagcctgatc ttagcgcaac tcttttcaaa
    55981 aattgcaggt caactgagcc agaccaatcc tgcttctgat ttgctagatg aaattgaacg
    56041 ttatctggaa gtcttccgga accgcagtct gcccaaaaca agagaagagt ttgaaacccg
    56101 cgccaccctt cttcaactcc tacgtgaagc caaaaccttc atccaagtaa aagttgattt
    56161 ttaccaaaaa tatagacagt aaaaaataaa accactaaaa aacagagctt gccagcattc
    56221 ttgaaaagta gtataattat tgcatgcgca atcatcttgt gacgcagaga ctgcccgtta
    56281 atggactttc ttctatttat gactctaaaa agaaaggaga cactatgacc tatttggaaa
    56341 aatggtttga cttcaatcgg cgtcagaaag aaattgaaag tctcttggaa gagaccattg
    56401 cccaacagag cgagcaaagt ctgaccttga aagagttcta cctgctctac tatctggact
    56461 tggctgaaga aaaatctcta cgccagattg acctgccaga taagcttcat ctgagcccga
    56521 gcgcagtttc taggatggtg gctcgcttgg aagctaaaaa ttgcggtcta ctcagtcgca
    56581 tgtgttgcca tcaagataga cgctctagtt ttatctgcct gacaaatgat gggcaaaaga
    56641 cactggcctc gctacaaaag actgtcgaag aaagcttgga agcaggtttg gatttcttga
    56701 tttaagatga ttttaaaaaa agttgcgtgc gcaatcattt ttcttgacat tctcttttat
    56761 aaggagtaaa ataaagttag cattaaacaa aggagtttta aacatgattg aaattaccta
    56821 tatagatgcc agcaagaacg aaagaactgt gactttcgag tcttatgaag actttgaacg
    56881 ttcgcaacaa gcttgcctta tcggcgtcgc agactactac cctgtccaaa aattaactta
    56941 caagggtcat aatttggact accatgggac ttatggagat atcttcttct atctcatgaa
    57001 acaagattta agccaatata actaaaaaaa ggagaaatac aatggcaaaa gcaattacag
    57061 atgcaacatt cgaacaagaa acaaaagacg gtttggtctt agtagacttc tgggcaactt
    57121 ggtgtggtcc atgtcgtatg caaggtccaa tcttggacaa attgtctgaa gaactttcag
    57181 aagatgtctt gaaaatcgtt aaaatggacg ttgatgaaaa tccaaacaca gctcgtgctt
    57241 ttggaatcat gtctattcca actcttctct tcaaaaaaga cggccaagtt gtcaaacaag
    57301 ttgcaggtgt tcacacagca gaacaaatca aggccatcat tgctgaattg agctaatcaa
    57361 acgagagacc aagtactttc tttggtctct tttttcttgc cctttgccat ttttcaaaaa
    57421 atatgctaga ctatagcaga ttgaaactag aatagtacac ctctacttct aaaacattgt
    57481 tagaaatcga tttgac
//
LOCUS       DAVLXF010000003        48661 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLXF010000003 DAVLXF010000000
VERSION     DAVLXF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.003"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(266..1252)
                     /locus_tag="VYO12_000190"
     CDS             complement(266..1252)
                     /locus_tag="VYO12_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1586860.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1262..2062)
                     /locus_tag="VYO12_000191"
     CDS             complement(1262..2062)
                     /locus_tag="VYO12_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1586861.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2315..3157)
                     /locus_tag="VYO12_000192"
     CDS             complement(2315..3157)
                     /locus_tag="VYO12_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1586862.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(3159..4451)
                     /locus_tag="VYO12_000193"
     CDS             complement(3159..4451)
                     /locus_tag="VYO12_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1586863.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4562..5833)
                     /locus_tag="VYO12_000194"
     CDS             complement(4562..5833)
                     /locus_tag="VYO12_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1586864.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6382..7899
                     /gene="malQ"
                     /locus_tag="VYO12_000195"
     CDS             6382..7899
                     /gene="malQ"
                     /locus_tag="VYO12_000195"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1586865.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7925..10183
                     /gene="glgP"
                     /locus_tag="VYO12_000196"
     CDS             7925..10183
                     /gene="glgP"
                     /locus_tag="VYO12_000196"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1586866.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYKVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11228..11800)
                     /locus_tag="VYO12_000197"
     CDS             complement(11228..11800)
                     /locus_tag="VYO12_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586867.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLTQLWDKNDAIPNNGRINF"
     gene            complement(12425..13273)
                     /locus_tag="VYO12_000198"
     CDS             complement(12425..13273)
                     /locus_tag="VYO12_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1586868.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13425..13703
                     /locus_tag="VYO12_000199"
     CDS             13425..13703
                     /locus_tag="VYO12_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586869.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13705..15768
                     /locus_tag="VYO12_000200"
     CDS             13705..15768
                     /locus_tag="VYO12_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1586870.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15846..17102)
                     /locus_tag="VYO12_000201"
     CDS             complement(15846..17102)
                     /locus_tag="VYO12_000201"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1586871.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17244..19709
                     /locus_tag="VYO12_000202"
     CDS             17244..19709
                     /locus_tag="VYO12_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1586872.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLCFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLATADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19774..20685
                     /locus_tag="VYO12_000203"
     CDS             19774..20685
                     /locus_tag="VYO12_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1586873.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20915..21613
                     /gene="dapD"
                     /locus_tag="VYO12_000204"
     CDS             20915..21613
                     /gene="dapD"
                     /locus_tag="VYO12_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1586874.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21681..22811
                     /locus_tag="VYO12_000205"
     CDS             21681..22811
                     /locus_tag="VYO12_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1586875.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22823..23362
                     /locus_tag="VYO12_000206"
     CDS             22823..23362
                     /locus_tag="VYO12_000206"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1586876.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVAYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23346..24023
                     /locus_tag="VYO12_000207"
     CDS             23346..24023
                     /locus_tag="VYO12_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1586877.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(24090..24989)
                     /gene="galU"
                     /locus_tag="VYO12_000208"
     CDS             complement(24090..24989)
                     /gene="galU"
                     /locus_tag="VYO12_000208"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1586878.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYKRTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(25011..26027)
                     /locus_tag="VYO12_000209"
     CDS             complement(25011..26027)
                     /locus_tag="VYO12_000209"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1586879.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            26239..26679
                     /locus_tag="VYO12_000210"
     CDS             26239..26679
                     /locus_tag="VYO12_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1586880.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            complement(26802..27218)
                     /locus_tag="VYO12_000211"
     CDS             complement(26802..27218)
                     /locus_tag="VYO12_000211"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1586881.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(27286..27936)
                     /gene="phoU"
                     /locus_tag="VYO12_000212"
     CDS             complement(27286..27936)
                     /gene="phoU"
                     /locus_tag="VYO12_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1586882.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(27951..28703)
                     /gene="pstB"
                     /locus_tag="VYO12_000213"
     CDS             complement(27951..28703)
                     /gene="pstB"
                     /locus_tag="VYO12_000213"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1586883.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(28705..29520)
                     /gene="pstA"
                     /locus_tag="VYO12_000214"
     CDS             complement(28705..29520)
                     /gene="pstA"
                     /locus_tag="VYO12_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1586884.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDIMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(29513..30376)
                     /gene="pstC"
                     /locus_tag="VYO12_000215"
     CDS             complement(29513..30376)
                     /gene="pstC"
                     /locus_tag="VYO12_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1586885.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(30494..31369)
                     /locus_tag="VYO12_000216"
     CDS             complement(30494..31369)
                     /locus_tag="VYO12_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1586886.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(31536..32867)
                     /locus_tag="VYO12_000217"
     CDS             complement(31536..32867)
                     /locus_tag="VYO12_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1586887.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(32868..33575)
                     /locus_tag="VYO12_000218"
     CDS             complement(32868..33575)
                     /locus_tag="VYO12_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1586888.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(33659..34144)
                     /locus_tag="VYO12_000219"
     CDS             complement(33659..34144)
                     /locus_tag="VYO12_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1586889.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            34130..34669
                     /locus_tag="VYO12_000220"
                     /pseudo
     CDS             34130..34669
                     /locus_tag="VYO12_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(34984..36675)
                     /locus_tag="VYO12_000221"
     CDS             complement(34984..36675)
                     /locus_tag="VYO12_000221"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1586890.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            36811..37257
                     /gene="argR"
                     /locus_tag="VYO12_000222"
     CDS             36811..37257
                     /gene="argR"
                     /locus_tag="VYO12_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1586891.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            37308..39842
                     /gene="mutS"
                     /locus_tag="VYO12_000223"
     CDS             37308..39842
                     /gene="mutS"
                     /locus_tag="VYO12_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1586892.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            39989..41683
                     /gene="patA"
                     /locus_tag="VYO12_000224"
     CDS             39989..41683
                     /gene="patA"
                     /locus_tag="VYO12_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1586893.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEVIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(42013..42276)
                     /locus_tag="VYO12_000225"
                     /pseudo
     CDS             complement(42013..42276)
                     /locus_tag="VYO12_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            42470..44236
                     /gene="patB"
                     /locus_tag="VYO12_000226"
     CDS             42470..44236
                     /gene="patB"
                     /locus_tag="VYO12_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1586894.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTENKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            44375..45064
                     /locus_tag="VYO12_000227"
     CDS             44375..45064
                     /locus_tag="VYO12_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1586895.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQIGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            45068..45247
                     /locus_tag="VYO12_000228"
     CDS             45068..45247
                     /locus_tag="VYO12_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586896.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            45234..46583
                     /locus_tag="VYO12_000229"
     CDS             45234..46583
                     /locus_tag="VYO12_000229"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1586897.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            46707..48167
                     /locus_tag="VYO12_000230"
     CDS             46707..48167
                     /locus_tag="VYO12_000230"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1586898.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYSRHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            48181..48615
                     /locus_tag="VYO12_000231"
     CDS             48181..48615
                     /locus_tag="VYO12_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1586899.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        14333 a         9254 c        10563 g        14511 t
ORIGIN      
        1 cgtcagtttc atctacaacc tcaaaaccat gttttgagct gacttcgtca gttctatcca
       61 caacctcaaa acagtgtttt gagcaacctg tggctagctt cctagtttgc actttgattt
      121 tcattgagta taaaaagatc ttgggtaaca actcaatttc agtagaaaag gaagtaaatc
      181 ttcccacaat aaaacgcata gtatcaagtt ttttcaatac ctgatactat gcgtttttgc
      241 ttttaacgac tttttgccca gtctcttatt tttcgataat tttgtgggcg atcaattgac
      301 ggtaacaaat ttgtttattg tttttattat cattaataat ctggagaata gtttcaaggg
      361 aaacacgacc aagctctaaa ctattgatat cgacataggc tgccaagttg agcttgggat
      421 taaccgagtc aaagctgaga acagggacat ccagctggtg tttggcaata tagttacaaa
      481 caccttcagc taggaggcta tcggttgtga tgatagcatc aatttgtgga tcgtgcttga
      541 ataatcgctt gctaaattta tagccctttt cttccagaaa ctcgtcggca aagtagatgc
      601 gattgttgtc agtggtaagt ttgtaatgtt taagcgcctg ttcatagcct gttaaacggt
      661 ctttggtcac gaagagcttt ttacttcctc cgataaaggc aatgcgtttg cagccttttt
      721 tgatgaaata ttcagtcgca tcaaaaccag cttgaacatt gtcgttgtcg acaagtggga
      781 tgaaaggaga tagagattta cctaagataa ggaaggggaa ctgttcttct gcgacgagtt
      841 ttacgagagg gtcttcttct tgggcataga gaaagattag cccatctaca cgcttgccgt
      901 agaccatttg tgaaatagcg ttgagacgct ccttctcatc tttccctgtt gctatctgaa
      961 tggcatagtg gttttcagat gcgacttgag agatgccacg tagaaccgat ggaaagaaag
     1021 gattctggta gaaggcgtct gagtcatcag gaagaactaa tccgataacc tgagtatagc
     1081 tgcttaccaa gctacgagcg ttgaggtttg ggtggtagtt gagttccttc atagctttgc
     1141 gaacgcgttt ttttgtttcg tcgctaatgg ttgatttatt ttgaataaca cgggttacgg
     1201 ttgaaggcga aacaccagca gccttggcca cgtctttaat cgtaacgggc ataaaaatct
     1261 cctatttatg gtaatttgga tcacggaaat gtgttttata aaagatagtg accagataca
     1321 gaacaaaaag aatattttgt acagtaatca gggttgtcat attttggcca aataatccca
     1381 aaataagtgt aatcagagtc ggcaatccta aacagttcaa gataaagtga tagcactctt
     1441 taaaggttct aaatgaaaag aggcgtgatt tcttagtgat ataaaggaga agactagctc
     1501 ctagagagac gataaagaaa ttcaaaccaa agaggaagct cgcaccgaga actaggaaga
     1561 ggctgatata gacacgattt tgttggtacc agtctttaga aattgcttgg gtcaagctgt
     1621 ctttgctttt gaaactctca gtctgaatgg ctcggtaaga gatgcgggtc agttccttgc
     1681 tttccttgct gatgactagc tcatttgtat cgaaatgcag ttgcaagtcc ttaggtaatt
     1741 ccttgatttg acttggacca atcacaacag aaggggcttg actagctgtt ccagtataag
     1801 ttaatgtgcc atcgacaatt gtagcatgtt cagagagatc ctggacaacc tcatttgtca
     1861 gaggttcata gacattatcg ataaaagttt ctagcggata ggtctcctgg gagctgtttt
     1921 ggatagcaat gggtaccata gacaagctga taaggaagat actggtaaag agaagttgaa
     1981 accagttgag ctcgaaacgt ttggacaggg gcttacgaaa cccccaaata cttgaaaaat
     2041 aggaaaatgg atatggaagc ataggtatct ttctaaaagg tgttttctat atgagtatta
     2101 ttatatagag aaatgctctt tatttcaagt ctaggagaaa aattgcttgc tacaaggata
     2161 ttatttacag tgacaattgc taataatcaa tgaaaatcaa agtgcaaact agccacaggt
     2221 tgctcaaagc acaactttga ggttggagat aaagctgacg tggtttgaag agattttcga
     2281 agagtataaa atgaaaaagg gtggcgggga taaattatcc cttgtcgcca ccacttgtaa
     2341 gtcctgaaac aaagttcttt tgtaggaaga agaagagaat acagattgga agggcgatga
     2401 ggatagcacc tgctgagaag taggcaatct tcaagttttt cgcattgtta acgaaggttt
     2461 ggagacctac ggcaacagta aagtattctt tctcacgaag caagaaacta gagaggatgt
     2521 agtccccgaa aggtcccatg aaggcccaga gagcttgtac ggcaaccatt gggcgaacaa
     2581 gtggtagaac aatttgccag aagcggcgga agtgtcctgc accgtctagt tttgcagatt
     2641 cgtctaaaga cattggcact gtatcgaagt agcctttcat gagccaagca ttcatcggga
     2701 taccaccacc aacgtagagg aagatgagga accagttgtg gttaagggcg ttcaacataa
     2761 gcgccataac gaagaaggct gtcaaagcgg ccatagttgg caccatttgg atgatcaaga
     2821 agaagaccaa actttgttta cgagccaaga agttgtaacg gctgtaggca taaccagcaa
     2881 gtacgatgat acttgtttga acagccatgg taattaaggc gataatcaaa gtgttgaggt
     2941 accaagtacc gtacaaggtt tcagtgaaga ggcctttaaa gttatcaaaa ttgaggtcga
     3001 tattagtatc tagtttaaag gctgagacgt tacctgcttt aaaggctgac ataatggtaa
     3061 tcaacagtgg atagataatt acaattgata gaccaatcag gtaaaggtaa gtaaggcttt
     3121 gagtcagtct acgtttgagt ttaattgagt tattcatctt agacgtcctc catatcaaat
     3181 gcgtgtagtt tcttgaatgc gatcatagag attgagatga caatgataga gataatcaag
     3241 gtaacagctg ccgccattga gtattgagga gatgtacctg ttgtcaaacg gtagatccat
     3301 gagatcaaga tatcggttga accagctcca cctccgacac taccaggtcc tccaccattg
     3361 aagaggtaca tgatagagaa gttgttaaag ttgaaggtgt attggctaat caaagtaggt
     3421 gccgcaacag ccaaaatcat tgggaaagtg atgttgcgga atttttgcca agcgttggca
     3481 ccgtcaatat aagctgcttc gtaaaggtcg ttaggaatag attgcaagat acccaaagtc
     3541 aaaacgtaga tgtatgggaa tccgagccaa ccttgcatca taatcaaggc aatcttagtc
     3601 caagttgggt ctgttttcca aggaataaga gctccatcaa ggaaaggaag gaatttagcc
     3661 aagattggca atacttgagt gttgatagca ccgacactat cgttaaacat gtttgagaat
     3721 gtcaagatag tgatgaaggc tgggacagcc caaggaagaa ggaaaataac accaaagata
     3781 cgttttcctt tgataaatgg ttggttggca atgatagctg tgaagatacc aattacgatt
     3841 tgtaaagtag aagctgccaa agcccaaatg atagtccaag aaagaacaga accaaaggca
     3901 gaacggaagg tactcaagct ccaaatgttt gtaaagttgg tcaaaccaac ccagtccaac
     3961 aacttgtttg gtggcaagtg ttggaagtcg tagttggtaa aggcgatcat caaggttacg
     4021 ataactggga agataatcgc gaaggtcatg gcaacataag atggaatgat caagaggtaa
     4081 gggaagccat tttcataaat ccctttgatc atgtctttga gtgtgcgtgg aactggaatt
     4141 ccattgttaa tgcgttttgc aatcgtatgt gcatctttga tatttgagaa ataaaagagt
     4201 acataaacga ttacaaagat tagatggaag gcaccacgaa tcagcataaa gagggaatta
     4261 tcacgacctg gtttgtcacc aagagtgatg aggttgctca attcaggggt tgcaagtgct
     4321 aggaagtaaa ggacaaatac gatggttaca ccaaggaaga taaaaccttt ggctttttgt
     4381 ttattgtaaa tctgtcctaa cccaggaatg atagacagca gggctgcttt actaggttgt
     4441 tgcttttcca tattaactcc tttcatagga tactactttt ttataagaaa gctaaattat
     4501 ttgtgtctct attgataaat tcaaaggggg atttgatttc cacccccctt gaacaaattt
     4561 tttattcacc aaatttttgt ttgattgttt ctttgatcaa tgttacagca tcgttagcag
     4621 ctgttttagc atctttttga ccacttacag catcaaagag catatttttc gctggatccc
     4681 aaactgcaga catttgagag atgtttggca gtggttgagt gttcttgaac tgtttgataa
     4741 cagctgttgt caactcatcg tttttacctt cagcgtatga acgagcctca gtattagctg
     4801 ggatttcgtt agtcttatca tataatactt tttgttgttc agttgcaaca aggaagtcta
     4861 caaatttttg agaagcttca aggttcttaa cggcttgagg aatgacccaa gctttaccac
     4921 caccgaatgc agcatattct tttccatttg gaagagttgg gatagttgca actccgtagt
     4981 ttactttagc atctttaaag gcttgagctt tccaaggtcc gtcgatgata gcagctgttt
     5041 taccttcttg gaattgagtt tggattaagt ttccagcacc ttctgtatct tgcatacctt
     5101 taggccattt ttcgtaccaa gatttagcgt agttgatacc tgcgatagaa ccgtcgtttg
     5161 caagaccgat gtctttagcg tctttaccgt tttggccaaa gacgtaagca ccgttaccgg
     5221 caagaagtcc atatgtatag tagaagtttg tccagtcagc taggaaggca gtagttttac
     5281 catcttcacc agcgaatgcg tatttgctat ctttagcaag gttttccaag tcagcaaatg
     5341 tttttggagc atctttcacc aagtctttgt tgtagtacat aacaagtgac tcgataacgg
     5401 caggagcacc gtaaacttta ccattagcag ctgttacaag agatttagtt gtgtcgtctg
     5461 ttttagcacc atcgctcaat ttcacttctg aaagttgtcc gtcagaacca aggctaccta
     5521 cacggtcgta tggagccatc ataacatcag ggacattacc agattggttg tcaagagaaa
     5581 gtttatcaag acctcctaga gcatcaccag ttttaagagt gacttttact ccagcttctt
     5641 tttcataagc tttagcaacc tcttcaatat agctcttata tccctcgtct acatatacag
     5701 tgagttcttt gacttcagat gaaccagaat cagcaggctt atcagcagtt ttgcttccgc
     5761 aagctaccaa aagcaagcta gcaagtgtag cagttccaag caccgcagtg ctcttcataa
     5821 atttagatga catagtgtat tcctcctaaa gaatagcaag ttttattgat aaggaaacgc
     5881 aaacgttttc ctttatgagc ttagtatagc acaaatagaa aacggttgca agtatttttt
     5941 gtaaaaattt taaaaaattt ttaagcttga tttgatagta taattttgca ttttaataga
     6001 agaaaagtat gaaatagata agaatacagt atttttaata aaaatatatg gaatctgtct
     6061 aaagagggaa atcaggaggg gtacctccct gtttctaatc tatatctatt ttctatagac
     6121 gtttgtgctt tgttgcatat aaattcaagc tccctaagga ggttttgtac tttgtatact
     6181 gtctcgtgag agtcaaactt taggattaga tagttttaag ttatctttgc taaaatgagt
     6241 tagtctcctt aaaaataatc aaaaaagttt tttaaaaacg cttgcaatta tgcttgaaaa
     6301 ggagtatact tataagtaac gcaaacgttt gcgtctgaaa aatacgcaac gttccattat
     6361 tttaacacac gaggtgctat tatgaaaaaa cgtcaaagtg gtgtgttgat gcacatctct
     6421 tctcttccag gagcttacgg aatcggatca tttggtcaaa gtgcttacga cttcgttgat
     6481 ttcttggtcc gtacaaaaca acgttactgg caaatccttc cattaggagc aactagttac
     6541 ggggattctc cttaccaatc tttctcagcc ttcgcaggaa acactcattt tatcgattta
     6601 gatatcttgg tggagcaagg tttgttggaa gcaagtgacc ttgaaggagt tgactttggt
     6661 agcgatgcgt ctgaagttga ctatgctaaa atctactatg cacgtcgtcc tcttttagaa
     6721 aaagcggtga aacgtttctt tgaagtcgga gatgttaaag attttgagaa atttgctcaa
     6781 gacaaccaat catggcttga gctctttgct gagtatatgg ctatcaaaga gtattttgac
     6841 aatcttgctt ggactgaatg gccagatgca gatgctcgtg ctcgtaaagc ttcagcactt
     6901 gaaagctatc gtgagcaatt ggcagacaag ttggtttacc accgtgtgac tcaatacttc
     6961 ttcttccaac aatggttgaa attgaaagct tacgctaacg acaaccacat cgaaatcgtt
     7021 ggggacatgc caatctacgt agcggaagat tcaagtgata tgtgggcaaa tccacatctc
     7081 ttcaaaacag atgtcaatgg taaggctact tgtatcgcag gatgcccacc agatgagttt
     7141 tctgtaactg gtcagctttg gggtaatcca atctatgact gggaagcaat ggacaaagac
     7201 ggctacaaat ggtggattga acgcttgcgt gaaagcttca aaatctacga tatcgttcgt
     7261 atcgaccact tccgtggctt cgaatcttac tgggaaatcc ctgctggttc cgatacagca
     7321 gcacctggtg agtgggtgaa aggtccaggc tacaagcttt ttgcagccgt taaggaagaa
     7381 cttggtgagc taaacatcat cgcagaagac cttggcttca tgacagatga agtgatcgaa
     7441 ttgcgtgaac gtactggctt cccaggaatg aagattcttc aatttgcctt caacccagaa
     7501 gacgaaagca ttgatagccc acacttggca cctgctaact cagttatgta cacaggaaca
     7561 cacgataaca atacggttct tggttggtac cgtaatgaga ttgatgatgc gactcgtgag
     7621 tacatggctc gttacacgaa ccgtaaagaa tacgaaacag tggtacacgc tatgcttcgt
     7681 acagtatttt catcagttag ctttatggca attgcaacta tgcaagattt actagaattg
     7741 gatgaggcag ctcgtatgaa cttcccatct acccttggtg gaaactggtc ttggcgtatg
     7801 actgaagatc aattgacacc agctgtcgag gaaggtttgc ttgacttgac aacaatttat
     7861 cgccgaatta atgaaaattt ggtagattta aagaaataag acaataatca ggagacaact
     7921 aaacatgtta tcactacaag aatttgtaca aaatcgttac aataaaacca ttgcagaatg
     7981 tagcaatgaa gagctttacc ttgctcttct taactacagc aagcttgcaa gcagccaaaa
     8041 accagtcaac actggtaaga aaaaagttta ctacatctca gctgagttct tgattggtaa
     8101 actcttgtca aacaacttga ttaaccttgg tctttacgac gatgttaaaa aagaacttgc
     8161 agctgcaggt aaagacttga tcgaagttga agaagttgaa ttggaaccat ctcttggtaa
     8221 tggtggtttg ggacgtttgg ctgcctgctt tatcgactca attgctactc ttggtttgaa
     8281 tggtgacggt gttggtctta actaccactt tggtcttttc caacaagttc ttaaaaacaa
     8341 ccaacaagaa acaattccaa atgcatggtt gacagagcaa aactggttgg ttcgctcaag
     8401 ccgtagctac aaagtaccat ttgcagactt tactttgaca tcaactcttt acgatattga
     8461 tgttactggt tatgaaacag cgactaaaaa ccgcttgcgt ttgtttgact tggattcagt
     8521 tgattcttct attattaaag atggtatcaa ctttgacaag acagatatcg ctcgcaactt
     8581 gactctcttc ctttacccag atgatagtga ccgtcaaggt gaattgctcc gtatcttcca
     8641 acaatacttc atggtttcaa acggtgcgca attgatcatc gacgaagcaa tcgaaaaagg
     8701 aagcaacttg catgaccttg ctgactacgc agttgtccaa atcaacgata ctcacccatc
     8761 aatggtgatt cctgaattga ttcgtctttt gactgcacgt ggtatcgatc ttgacgaagc
     8821 aatttcaatt gttcgtagca tgactgccta cactaaccac acaatccttg ctgaagcgct
     8881 tgaaaaatgg cctcttgaat tcttgcaaga agtggttcct cacttggtac caatcatcga
     8941 agaattggac cgtcgtgtga aggcagagta caaagatcca gctgttcaaa tcatcgatga
     9001 gagcggacgt gttcacatgg ctcacatgga tatccactac ggatacagtg ttaacggggt
     9061 tgcagcactc catactgaaa tcttgaaaaa ttctgagttg aaagccttct acgaccttta
     9121 cccagaaaag ttcaacaaca aaacaaacgg tatcactttc cgtcgttggc ttatgcatgc
     9181 taacccaaga ttgtctcact acttggatga gattcttgga gatggttggc accatgaagc
     9241 agatgagctt gaaaaacttt tgtcttatga agacaaagca gctgtcaaag aaaaattgga
     9301 aagcatcaag gctcacaaca aacgtaaatt ggctcgtcac ttgaaagaac accaaggtgt
     9361 ggaaatcaat tcaaattcta tctttgatat ccaaatcaaa cgtcttcacg agtacaaacg
     9421 ccaacaaatg aacgctttgt acgtaatcca caaatacctt gacatcaaag ctggtaacat
     9481 ccctgctcgc ccaattacaa tcttctttgg tggtaaagca gctccagcct acacaatcgc
     9541 tcaagatatc attcacttga tcctttgcat gtcagaagtt attgctaacg atccagcagt
     9601 agctccacac ttgcaagtag ttatggttga aaactacaac gttactgcag caagcttcct
     9661 tatcccagca tgtgatatct cagaacaaat ctcacttgct tctaaagaag cttcaggtac
     9721 tggtaacatg aaattcatgt tgaacggagc tttgacactt ggtactatgg acggtgctaa
     9781 cgtggaaatc gctgagttgg ttggagaaga aaacatctac atcttcggtg aagattcaga
     9841 aactgttatc gacctttacg caaaagcagc ttacaaatca agcgaattct acgctcgtga
     9901 agctatcaaa ccattggttg acttcatcgt tagtgatgca gttcttgcag ctggaaacaa
     9961 agagcgcttg gaacgtcttt acaatgaatt gatcaacaaa gactggttca tgactcttct
    10021 tgacttggaa gactacatca aagtcaaaga gcaaatgctt gctgactacg aagaccgtga
    10081 cgcatggttg gataaagtca tcgttaacat ttctaaagca ggattcttct catctgaccg
    10141 tacaatcgct cagtataatg aagacatctg gcacttgaac taatactctt cgaaaatctc
    10201 ttcaaaccac gtcagcttta tctgcaacct caaagcggtg ctttgagcaa cctgcggcta
    10261 gcttcctagt ttgctctttg attttcattg agtataagat gcaaatttat actaatacat
    10321 tttgtaaaaa agcgagtttc gattgaaatt cgctttttta aatgctgtgg atttgggtca
    10381 atcttgtcta aaaataggga aatcctagat acagtgaagg ctttaaatgc tggtttttac
    10441 tgtcttcagc cttatatttt ttcgtagttg gttacctcat atctcttgta ttcgcttaca
    10501 taaagtatta taatataatt gtaggaaaga aggtgttttt atgatataca cacttaaatt
    10561 ggtgttgttt attacctttc ttgtaataag cttgttacct gataagattt ttggaaaaaa
    10621 taaaaaaatt tggaaaatag tttttgcaat attgacggca gtggcagcat ttatatttat
    10681 gtactaagtt attttaagaa tgtagggaaa taaaccctac attcttttta gttttttctg
    10741 ttttctaaat tctatttatc caagcgcttc aacatttctt gcttcttcgc ttcaagttct
    10801 gcacgctttt cttcgatttc ggcatgtttt ttctcgagtt cagaacaact tgcaccattg
    10861 ctaaattctt ttcgccatca ggagataggg tgagtcgaca tgtctattac tcacccaaag
    10921 cagtcctaca aagcaggaat tttctgttac ttttttggaa atagtaacgt ttatacagct
    10981 ttgacacttc gtatcaaagc gccaaacaca ctccgaggga tttacagaaa gcagaaaagg
    11041 aatgatctgg tataagatca ttccttttct ctctttttct ttaagtaatt atatacaatg
    11101 tacgacgaag tcgtcattgc aatgctgatc caccacctaa agggaacttt aaacaacatt
    11161 gataagataa agaatataaa caacgaaaat acgttatacc caattaattt tattgtatat
    11221 ctcatgatta aaagttaatc cttccgttgt taggaatggc atcattttta tcccataatt
    11281 gtgttaaata agtccccggt gataataaat tcatagcgga ttctaaagca acatcattta
    11341 caaaccaact acctagatat ctagaaattg ctgaacgaat agcacttttt gctgcatgtt
    11401 ttccttttac tttaattaga tttgcaaggc ctgcagtagt tcctcctaat gctaaagcta
    11461 ttgcagtatc taatagagca cccatttgat taactgtaat accttgccaa gctgctctaa
    11521 atggagagta tgtaggtggg attgtataat cgccttgtaa ttgtcggtta attacttctt
    11581 tgatccattg ttgtgagacg tctggatgaa aagattggat ttcgtttgca agtgtattga
    11641 tttgttcttc tgttagagaa gtgacaggtt gaagttccat atttgtttca atttgtgata
    11701 cttgttcaga agcgtataca gctgaaacac ttggaatcgc tgatacaatt aacacaattg
    11761 acgtcaaaaa aaccgaaata aatttcatta atttgttcat gagcttttct cctttttatt
    11821 tgtatttgct tacattttat tatatactgt tattatagtc aagaaaaata tgctattatg
    11881 ttaaaaaaat atttttcaaa atataaatgg acggatttat tttggattct ttttgtaatt
    11941 ggggcatgcc tcttgacggg taatagtagt cttttccccc ttacccatca agaattctct
    12001 tttcgtggta gcgtttgggg tctggtactg gccttatatc acttattgtt cattgataag
    12061 tttgtaattt cgaatcgaaa ataaaaatta gaactatgat tgactggcta cttttaatat
    12121 tgattttgga ggaagatttt gtctctatta tttattattt taaatttatc tattttgtac
    12181 aagatttatt ctttgagacg ggagaagtcg aaatacttga tttatacggc ctatatcata
    12241 tttggggtaa atgtagtata tggtattcaa tggttattaa aagaactgat ttcaactatt
    12301 tccacttaat atgtatgaaa actataacaa aacgcataag attaaggttt ttaagcacct
    12361 gttcttatac gtttttgtaa tattgaagat tttcggtaag ttttctactt ttgcgaagtc
    12421 tagtttagaa tgctttccca accaaaatct ctgcttcgat ggtaatctct gtcagttggc
    12481 tccagtcaat cttgctctgg tcaacgtgaa agaggagagg agtcatactg agtagggctt
    12541 ggagttgttc tgctgtgata gtcttagtca gagaggcagt ttgactagat aggatggtaa
    12601 agtgttcctg gaaatgctcc ttgatatctt ggttagaata ctccttgttt gtcagctggt
    12661 cctgtactct ttgacggatt tctttgaggt gattttcagt tgggataacc tttatcaaga
    12721 taccgtcttt ggataaaacg cgacgaaatt ctccatagtt ggcaggtgag aagatatcaa
    12781 gcagaatatc catgttagcg tcttttatag gaagtcgtgc caagtcgcca acgaaccaat
    12841 tgactgccca gttgggttca cttttagcag cgatttggac tgaatctttg gagatgtcaa
    12901 aggcatagaa agttttttca gagtgacttt cttgtagttt gcgagaatag aatccttcac
    12961 cacaaccgat atccaaaatt gttgtggtag tttttgagct tgcaagcaag tcagatacag
    13021 catctaagat agcttggtaa aagccggctt ctaggatttg ttgacggttt tgaaaatttt
    13081 ccttgtcata gttagcagat tgcttgattt gaggtgctag attgacatag ccaaatttcg
    13141 ccaagtcaaa agaatgacgg ttgcagcact tgaaattagt ctctaacaga gtcagatttt
    13201 cttgacagat aggacaggca aaggcagtcg cagaagcaaa acgctgaagt ttgggcttga
    13261 gatttgtatt catagtttaa gtgtatcaaa agaggcaaat gaaagcaact ttaggaataa
    13321 gtagatggga gattcagtag aaataaaact aattctccaa tttatagaat gaaattgctt
    13381 ttatatctaa attgctatat aataatgata aggaggaaat aagtatgtta aaagaagtat
    13441 taaccgttgc aaaagttgcg aaaaaatctt cattattttt gggtggtgtc gcatttggta
    13501 cccttggttt gaaaatctta gcaagtaagg aagctaaaaa aggttattct aaagctttgg
    13561 ctaaggctta caagttgaaa gacgagctag atgcatctgt ttctgttgtg aagcaacatg
    13621 gagacgatgt cttgcaagat gccaaatatt tgtacgagca agagaaaaaa gaagagcaat
    13681 tagatagcct tataggtgaa taatatgtct tttaaagtgc tacatagagg ataccaacat
    13741 atccgactat catcttcttt ttcacttacc ttggatattc aagactatct tcgttccttg
    13801 gcgagagatg aaaaggggat tgagtctatc cagttttaca tggatcaaca gcactttact
    13861 ctacgcataa aagaaggctt ttctgtatta gataatgcag aagccttttt aaaaagaatt
    13921 gataaaggga aagtttctga gttgatgact cttcccattc gtagagaaga gagtgcttat
    13981 tctattgttt caggtgcagc ggttaagcgt gtactttttc gtagttttgt gccgtatcct
    14041 attcgctata tatggacttg ttatcaggct ttgggttata ttagagaagc ctatcaaaca
    14101 ctagcgcgta aggaactaac gatggaagtc ttggactgtt cggctatttt attgtccttg
    14161 tttatgaacc aatccaagac agctagcaat atcatgttta tgcttgattt ggggaatcat
    14221 ttagatcagt ggtccttgaa aaaaactgca acagatttag aacagagtct tcttgcaaaa
    14281 gagagcgatg tattcctagt acagggcgat acggttgtta gtataaagag ttccgatgtt
    14341 caaataggag atgtcttgat cttatctcaa ggaaatgaaa ttctgtttga tgggcaagta
    14401 gtttcaggtt taggtatggt caacgaaagt tccttgacag gagagagttt tccagttgaa
    14461 aaaagagagt ctgatttggt ttgtgcaaat acagtattag aaactggaga gttacgcatt
    14521 cgtgtaacag ataatcagat gaacagccgt attttacagc tgattgagtt gatgaagaaa
    14581 tctgaagaaa acaagaaaac gaaacaacgc tatttcatca agatggcgga taaagtcgtc
    14641 aaatataatt tcttgggggc tgggctgact tacctattga caggttcttt ttctaaggct
    14701 atttctttcc tattggtcga tttctcctgc gctttgaaaa tctctactcc tgtagcttat
    14761 ttgacagtta tcaaggaggg gttgaaccgt gaaatggtga ttaaggatgg agatgttctg
    14821 gagaaatatc tggaagttga tactttcttg tttgataaga caggaacaat cacaactagt
    14881 tatcctatag ttgaaaaggt gttacctttt ggagactata gtgaggaaga tattctcaga
    14941 atcagtgcct gtcttgagga acacatttat catcctattg ctaatgccat cgtcaagcaa
    15001 gctgagatag agggaattga acatgaggaa atgcatggga aactccaata tatcgcaagc
    15061 aaggggatca aatctcatat agatggccaa ccagttctta ttgggaatta tgtcttgatg
    15121 caggatgagc agattcatat cagttcagaa caaaatgctt taattgaaga gtacaagagt
    15181 cactacaatc tcttattctt ggcttatcaa aatgaattga ttggaatgtt ctgcattcat
    15241 actcctttga gaaaagaagc aaaaacagcc ttggataaac ttaaggcaca ggggaaaaaa
    15301 ttgattctgg caacaggaga caccttgatt aggacagagg aattagtcaa agatttgccc
    15361 tttgatcagg tctatacaga cttgaaacct gatgggaaat ttgagttagt agagaaactg
    15421 cagaaagcag gtcacactat tttgatggtt ggagatggct tgaatgactc agcggcttta
    15481 actctatcag atatcggtgt ggtgatgaat gagagtgcag atatttctaa gcagatgagt
    15541 gatatcttat tgttagataa tcgtttggat ttcttccaag agttggattc gttatcatca
    15601 tctttgcaaa cactcatcaa gaaaaatatt caggataccg ttgtcgtaaa tagtagtttg
    15661 attggctttg gcttatttaa ttggttcagt ccttcaaatc tctctatctt acataatcta
    15721 acaaccttgc gcattgtcct gcgtagcctg tctattaaaa atagatagat gagagttatt
    15781 aacagcaaaa aggagttacc tttctcgagg ttaactcctt tgtttataga taaatgttga
    15841 atagtttagt aagtcaatac aaagtatttt ttcttcccac gacggataac agtcagttca
    15901 ttttctaact tatcagcgtc actcaagaca tagtcaaggt cttggatgcg gtcgccgttt
    15961 acgtagatgg ctccgttttg gacgtcttca cgggcttggc gttttgagtt aactatacca
    16021 gatgagacga gcagttccac gatattgttg ttttcgtctg cctgtacttg gtagtttggc
    16081 acaccacgaa gtccttgttt gagctctttg acagaaaggt ttttgatgtt tcctgcaaag
    16141 agttgctcag tgatgttaag tgcttctttg taggcttctt ctccgtgaac aagtgtaaca
    16201 acttcacgag ccaagacttt ttgagccaag cgttcgtgtg gcgctgcttc aaattgttta
    16261 cgaatatctt caatctcatc aagtgacaag aaagtaaaga ttttcaagaa gcgaacagcg
    16321 tcagcgtcca tcacgttcat ccagaattgg tacatttcgt atggagaagt cttttcggga
    16381 ttgagccaga cggcatttcc ttctgattta ccaaatttct taccagttgc atctgtgatt
    16441 agtggaacag tgataacgtg accagtcttg tccgccttac gacgaagcaa ttcggtacca
    16501 gctgtcatat ttccccactg gtcagaacca ccgatttgaa gagtgacatt atggtcttgg
    16561 ttaaggacga agaagtcgta cccttgcatg atttggtaag cgaactcagt gtaagaaatt
    16621 cctgtttcga tccgtttttt aacagattcc ttactcatca tgtagttgac cgtgaagtat
    16681 tttccaatat cacggaggaa gtcaatgaag ctgatgctgc caaaccagtc gtagttgttg
    16741 accatgacag ccttgttttc gccattttca aagtcaagaa aacgagaaag ttgtccttgg
    16801 atagacttga cccagccatc tactgtgtct tttgtttgga gactacgttc agcatctttg
    16861 aaggacggat ctccgatgag acctgtagca ccgccaacga gcgcataagg tttgtgacct
    16921 gctagttgca agcgacgact tgtcaagatt gcgacaaggt ggcctaggtg aaggctgtca
    16981 gcagttggat cgtagccagt atagtaagaa acttgacctt cttctagggc tttacgcaaa
    17041 gcttcttcat cagtcgtttg aaatatcaaa ccacgctctt ttagctcatc aaaaatgtgc
    17101 atgtgtcttt tctccttttt aaaattattg tttctaccta ttgtatcaca aacttgggca
    17161 atagcctagc agaataggga agaaagtggc tattttattg aaaatttccc ttttgtggta
    17221 taatagataa agtgaggaaa tccatgcaaa atcaattaaa tgaattaaaa cgaaaaatgc
    17281 tggaattttt ccagcaaaaa caaaaaaata aaaaatcagc tagacctggc aagaaaggtt
    17341 caagtaccaa aaaatctaaa accttagata agtcagccat tttcccagct attttactga
    17401 gtataaaagc cttatttaac ttactctttg tactctgttt tctaggagga atgttgggag
    17461 ttgggattgc tttggggtac ggagtggcct tatttgacaa ggttcgggtg cctcagacag
    17521 aagaattggt gaatcaggtc aaggacatct cttctatttc agagattacc tattcggacg
    17581 ggacggtgat tgcttccata gagagtgatt tgttgcgcac ttctatctca tctgagcaaa
    17641 tttcggaaaa tctgaagaag gctatcattg cgacagaaga tgaacacttt aaagaacata
    17701 agggtgtagt acccaaggcg gtgattcgtg cgaccttggg gaaatttgta ggtttgggtt
    17761 cctctagtgg gggttcaacc ttgacccagc aactaattaa acagcaggtg gttggggatg
    17821 cgccgacctt ggctcgtaag gcggcagaga ttgtggatgc tcttgccttg gaacgcgcca
    17881 tgaataaaga tgagatttta acgacctatc tcaatgtggc tccctttggc cgaaataata
    17941 agggacagaa tattgcaggg gctcggcaag cagctgaggg aattttcggt gtagatgcca
    18001 gtcagttgac tgttcctcaa gcagcatttt tagcaggact tccacagagt cccattactt
    18061 actctcctta tgaaaatact ggggagttga agagtgatga agacctagaa attggcttaa
    18121 gacgggctaa ggcagttctt tacagtatgt atcgtacagg tgcattaagc aaagacgagt
    18181 attctcagta caaggattat gaccttaaac aggacttttt accatcgggc acggttacag
    18241 gaatttcacg agactattta tactttacaa ctttggcaga agctcaagaa cgtatgtatg
    18301 actatctagc tcagagagac aatgtctccg ctaaggagtt gaaaaatgag gcaactcaga
    18361 agttttatcg agatttggca gccaaggaaa ttgaaaatgg tggttataag attactacta
    18421 ccatagatca gaaaattcat tctgccatgc aaagtgcggt tgctgattat ggctatcttt
    18481 tagacgatgg aacaggtcgt gtagaagtag ggaatgtctt gatggacaac caaacaggtg
    18541 ctattctagg ctttgtaggt ggtcgtaatt atcaagaaaa tcaaaataat catgcctttg
    18601 ataccaaacg ttcgccagct tctactacca agcccttgct ggcctacggt attgctattg
    18661 accagggctt gatgggaagt gaaacgattc tatctaacta tccaacaaac tttgctaatg
    18721 gcaatccgat tatgtatgct aatagcaagg gaacaggaat gatgaccttg ggagaagctc
    18781 tgaactattc atggaatatc cctgcttact ggacctatcg tatgctccgt gaaaagggtg
    18841 ttgatgtcaa gggttatatg gaaaagatgg gttacgagat tcctgagtac ggtattgaga
    18901 gcttgccaat gggtggtggt attgaagtca cagttgccca gcataccaat ggctatcaga
    18961 ccttagctaa taatggagtt tatcatcaga agcatgtgat ttcaaagatt gaagcagcag
    19021 atggtagagt ggtgtatgag tatcaggata aaccggttca agtctattca aaagctactg
    19081 cgacgattat gcagggattg ctacgagaag ttctatcttc tcgtgtgaca acaaccttca
    19141 agtctaacct gacttcttta aatcctactc tggctactgc agattggatt gggaagactg
    19201 gtacaaccaa ccaagacgaa aatatgtggc tcatgctttc gacacctaga ttaaccctag
    19261 gtggctggat tgggcatgat gataatcatt cattgtcacg tagagcaggt tattctaata
    19321 actctaatta catggctcat ctggtaaatg cgattcagca agcttcccca agcatttggg
    19381 ggaacgagcg ctttgcttta gatcctagtg tagtgaaatc ggaagtcttg aaatcaacag
    19441 gtcaaaaacc agggaaggtt tctgttgaag gaaaagaagt agaggtcaca ggttcgactg
    19501 ttaccagcta ttgggctaat aagtcaggag cgccagcgac aagttatcgc tttgctattg
    19561 gcggaagtga tgcggattat cagaatgctt ggtctagtat tgtggggagt ctaccaactc
    19621 catccagctc cagcagttca agtagtagtt ctagcgatag cagtaactca agtactacac
    19681 gaccttcttc ttcaagggcg agacgataat tctctaaatg aagtggccaa tcaatggatt
    19741 gccacttttt tcttttcgtg ttacaataag ggtatgaatc agtatcagaa aaagattgtt
    19801 aacggaaaaa tttattcgct cctatccggc ttaatatggg gaatctgtgg aattttagga
    19861 gagtacttct ttactcatta tcaggtgtct tcgggctgga ttacctctat gcgtttgaca
    19921 ctggcaggga gtcttgtact catttggtct gcaatacaat taaaatcgca agtgctagat
    19981 atttggcgag acaagaaaaa ttacctgccc tttttagcct atgctatttt ggggattttt
    20041 tcagttcagt attttttcta tctctgtgta gaatactcaa atgctacgac agcaactatt
    20101 ttacagttta ttagccctgt ctttatcctc ttttacaatc gcttggttta tcaaaaacga
    20161 gcgtcaaaaa gcgctgtttt ctatgttttg gttgccatgc tgggtgtttg cttgatggcg
    20221 acaaagggag acctctctca gttatccatg acgccgctag ctcttataac aggtttgctg
    20281 agtgccatgg gtgttatgtt taatgttatc ttgccccaac cttttgctaa gcgttatggt
    20341 tttgttccta cggttgggtg ggggatgatt ttggcaggtt tgtttagcaa tgtcctctcg
    20401 ccggtttatc agctttcctt tactcttgat atttggagta tcttgatttg cctcattatc
    20461 gctttctttg gaacggcttt tgcttttttc atttccatga aggctgtgtc cttggtttct
    20521 cctttggtgg ttgccgttat cagtgccagt gaacctctct cttctgctct cttgagtgtt
    20581 ttgttcttag gattagtagt ggattggtcc ctccttctag ctatagcctt gattatttta
    20641 cccatgatat ttttgtctat agaagaagcg aaagaaagta gataaaaagt cttttcttaa
    20701 ttctaaaagt gtgctatact ggaatagtca ataaaacacg gggagatagt tgtgaagagt
    20761 gtttttaggt tgtatcggta ggggcttgct tttaccgaca gcacgtttta tctcacatcc
    20821 ctaaaaaatc atacctaaaa acagacaaaa aggtttcaaa tttgaggcct tttttggtag
    20881 aataggtatc attataacga accaggaggc acctatgact gctacaaaaa tgaacgctca
    20941 agaaattatc caatttatcg ccaatgctga aaagaaaacc agtgtcaaag taacctttga
    21001 ggggcaactc gcaactgctg tgcctagctc tgttgtcaaa ctaggaaatg tcctattcgg
    21061 agactggaag gacgtggctc cgcttcttga aggtttggta gaaaatcaag attatgttgt
    21121 cgagcaagat gctcgtaatt ctgcagttcc tttgctagat aagcgtgcta tcaacgctcg
    21181 tatcgagcca ggtgctatta tccgtgacca ggtggaaatt ggtgacaatg ctgttatcat
    21241 gatgggagct gttatcaata tcggtgctga aatcggtgct ggaaccatga ttgacatggg
    21301 tgccatcctt ggtggccgtg ccattgttgg aaaaaatagc cacgttggtg caggtgcagt
    21361 tttggcaggt gtgattgagc cagctagtgc tgaaccagtc cgtgtcggag acaatgttct
    21421 catcggggct aatgcagtgg ttatcgaagg agtccaaatc ggtagtggtt cagttgtcgc
    21481 agcaggagct attgttaccc aagatgtccc agaaaacgtg gtagtagcag gtgttccagc
    21541 tcgtattatc aaagaaattg atgcccaaac tcaacaaaaa acagcgctag aggatgcgct
    21601 tcgtaccttg taattgtaaa agtaaaaaag aggcggaacc ctttttccag cctctttctg
    21661 ctatatcgga ggacagatag atgttagatt tgattcagac tagacgagat ttacaccaga
    21721 ttccagagat tggcttggag gagttcaaga ctcaggctta tttgctggat gtgattgaga
    21781 aattgactac gggcaaggat tttgttcaaa ttcgtacttg gcggacaggg attttggtct
    21841 acctgcaggg aagtcagccg gagcgaacca ttggttggcg gacagatatt gatggcctgc
    21901 ctatcgtcga acaaacaggt ctaccttttg cttctcaaca ccaaggtcgc atgcatgcct
    21961 gtggtcatga ttttcatatg accattgcct taggctgtct tgagcgcgcc cttgaggagc
    22021 aacccaagaa taatctgctc tttctatttc agcctgctga agaaaatgaa gctggtggta
    22081 tgctcatgta tgaggacgat gcttttggag attggttgcc agatcaattc tatggtctcc
    22141 atgttcgtcc agatttgaaa gtcggccaga ttgcgactaa tactcataca ctctttgcag
    22201 gaacttgcga ggtgaagatc cgtttcaaag gcaaaggtgg ccacgcagct tttcctcatg
    22261 aagccaatga cgccttggtg gcggctagtt actttgtgac ccaagtgcag tcagttgtta
    22321 gccgcaatgt caacccaatc gagggagcag tggtgacctt cggcgttttt caagctggaa
    22381 ccaccaacaa tgtcatcaca gacacagctt ttttacatgg aactattcgt gccttgacac
    22441 aggacatgag tctcttggtg caaaagaggg taaaaacagt tgcagaaggg gttgcagctg
    22501 cctttgatat ggaagtcgaa gtggaactca aacaaggtgg atacctacct gttgagaaca
    22561 atccagcctt ggcgcgtgaa ctgatggact tctttgacga gaaagacgga atcgagttga
    22621 ttgatatcga acctgctatg acaggcgagg actttggtta tctcctttca aaggttgatg
    22681 gcgtgatgtt ttggctaggt atcgatagtc cctacgctct tcatcaccct caaatgagtc
    22741 ctaaggaaga agtcttagct attggggtgg ctgcggtgtc tagtttcctg aaaaagaagg
    22801 cagcagaata gagggcttgt ctatgaaatc ggaattacgc aagcaagtct tgcatgaaat
    22861 gaaggctata tctcaagagc aaaaacaggc tatagaccaa gctttaaccg agcggctttt
    22921 acaccatccc ttctaccaag aagccaaggt catcgcaacc tacctctctt tttctcatga
    22981 gtttcaaacg cgggaactga ttgagcaggc gctgaaggac ggcaagaagg ttttaatacc
    23041 caaaacttat cccaaggggc gcatggagtt tgtggcctat gatccgcaac agttggtaaa
    23101 aacttccttt gccttactgg agccacaggg agatgtggaa gtggtggatg catctcagat
    23161 tgatttgatt catgttcctg gtctggcttt tacgacgaaa ggatatcgga taggatacgg
    23221 tggaggctat tatgaccgct atctggaaca tttttctggt cgtactttga gtacgatata
    23281 tccttgtcaa attcaggact ttatacctga aaaccatgat attcctgttc aggaggtatt
    23341 aattgatgaa ggaaatcttt gatagacgtt accctgtgac gagtttcttc ctcttagtga
    23401 cggccttggt atttttacta atgttggtca ctgcaggcgg aaactttgac agggcagata
    23461 cattatttcg atttggagcc atgtatgggc cagctattcg cctctttccc gagcaggttt
    23521 ggcgtctctt gtctgccatt tttgttcata ttgggtggga acatttcatt gttaatatgc
    23581 tttcacttta ttatcttgga aggcaggtag aggagatttt cggttctaag cagtttttct
    23641 ttctctatct tttatcagga atgatgggca atctctttgt ttttgtattt agtcctaaat
    23701 ccttagcagc aggcgcctct acctctcttt atgggctatt tgccgcgatt attgttcttc
    23761 gctatgcaac tcgcaatcct tatatccaac agctagggca atcctatctg acactttttg
    23821 tggttaacat tattggaagt gttctgattc caggaatcag cctagcaggc catatcggtg
    23881 gtgcagttgg tggcgcattt ctagcagtta tctttccagt tagaggagaa aaacggatgt
    23941 ataacaccag ccagagatta ggagcggtag tcttgttcgt aggactcgcc attttgcttt
    24001 tctacaaggg aatgggaatg tgacgaagtt gaaagaaaat ttgtgtaatg aaaaaagata
    24061 aggcattttt tgaaccttat ctttttattt tattccttct cagtcaattc ttttccaagt
    24121 tggatgaggt aattcttcaa atcatctttg acttgtggat gtttgagggc gtagtcaata
    24181 gatgttttca taaagccaaa cttatcccca acgtcgtaac gagctcctgt aaattcacgg
    24241 gcaaaaacac gctgtgtttt attaagagta tcgattgcat cagtaagttg gatttcattt
    24301 ccagcaccag gagcttggtt ttcgaggatt tggaaaattt caggcgtgag gaggtagcgt
    24361 ccgataatag caaggtcgct aggagcgtcc tctggagctg gtttttcaac aaaggtttca
    24421 acactgtaaa gaccatcttt tccttcgcct tgcggagcaa taaccccgta agcagatact
    24481 tcgtcatgag ggactggcat gacagcgata gtagacgcgt gggtacgctt gtagtcatcc
    24541 atgagttgtt tggtaagtgg aacagccttt tcgtctgtga tatccatcaa gtcatcacca
    24601 agcataacga caaaaggttc atttccgacg aaagccttgg cttgcaaaac agcatctccg
    24661 agaccgcgtg gatgagtttg gcggataaaa tgcagacgca tgccagttgt ttcatcaact
    24721 agcttcaaaa gatctgtttt ccctttttct ttgaggttat attccaattc gaagtttgaa
    24781 tcgaagtggt cctcaataga acgttttgat ttaccagtga caactagaat atcttcaata
    24841 cctgatttga gagcctcttc cacgataaac tggatagttg gtttgtctac gattggcaac
    24901 atttctttgg caagggcctt ggttgctggt aaaaatcgag ttcctagtcc agcagcaggg
    24961 ataactgcct ttctaacttt tgatgtcata aaaatccttt ctatagaggg ttaagaccac
    25021 tcattttctg ctttaaattc attgttcatg atgtcataaa tggcatcttt gatattggtt
    25081 ccgtgataaa taacttggta aatagcctgt gtaatgggca tatagactcc aagttcttgg
    25141 gctagttcat aggctgctcg agtcgttgaa attccttcga ttaccatgcc catattagct
    25201 tctatatcag ctagggattc tcctcgtccg agagcatctc cagctctcca gttacgagag
    25261 tggatggaag ttcccgttac gatcaaatct cccacaccag ataagccgct ataggtcaat
    25321 ggactggccc cgagtgctac ccctaggcgg gtgatttctg ctaaacctcg agcgatgatg
    25381 gctgccttag cattatcacc aaatccaaga ccatgtaaag ctccagcacc gacagcaata
    25441 atatttttaa gagcaccagc agtttcaacc ccgataacat ccgtattggt ataaagtcgg
    25501 aagtagtgat tactaaatag ctcctgaacg tattgagctg tttgtaaatc tttagaagca
    25561 gcagttatta aagttaggtc acgcacaatg gtctcttctg catgactagg ccctgaaaca
    25621 acgacgatat cactacggag atgttcagga atttcttctt caagaatggt tgataatcgt
    25681 ttatggctat caggttctaa tccctttgat gcgtgcatga tgataacctt atggtccaag
    25741 gtttgtgcaa cttgctgggc aacaagtcgt gtcacttttg ttgggacaac aaacaaaatc
    25801 gcatccacat ttttcaatgt ttctgctaag tcggtgtagg caatgatatt ttcgtctaga
    25861 acgacatctt taaagtagtg cttattagta tggtgtgtat taatttcatt gatttgctcg
    25921 ggaagatttc cccaaatacg tacctcgtgt ccattgtcat ttaagacttg tgaaagggcg
    25981 gttccccaag aaccaggccc caagacggcg acggtttgtt tttccatgat ttcctccttg
    26041 atagatttct ttctcttatt ctatcatatt ctaggggatt ttgcacttgc attactgggg
    26101 tatggtggaa aaatacacaa agaccgagat gaagggataa actcttagtc tttagttaaa
    26161 gtgagaagaa atctgataga tagtttgatt cagtatatgc ttataataga ggtaaacaac
    26221 tcaggaagtt cttgtaggtt gcgagagttt ggcgaaaaaa ttaaaagatt acgtttggct
    26281 aaaaaaatca gtcgttcaga attttgtggt gatgagtctg aattaagtat ccgtcaatta
    26341 attagaattg aaaatggaga atccagacca acactaacaa agttaaaata tattgctgaa
    26401 cgtttggagg ttgaagatta caagttgatg ccaagttata tagagttgga taaggaatac
    26461 ctagaattga agtatttctt gatgaggact cctacatacg aagatgaaac tatcgcccaa
    26521 aagaaagaga gtgtttttga taagattttt gaagagtatt atgataggct acctgaggaa
    26581 gaaagattta tcatcccaaa ttattcatat ctagcactag cgaactacac agttcaaaaa
    26641 ttaccagaaa agctagttga aatactgtcc ttctggtgat tttgcttaat ttctgatgtt
    26701 cgagacatga ttgaggagat ttgtcttgat tttttcctaa aaaagggcag actcctccct
    26761 tggtttgatg ctcgattttt aaaataaggt gacttattct gttaggcagt tttcccctgc
    26821 cctctagctc tatatggaac agggagtatc agatttatct ggcagattgt atcaaataag
    26881 gcttgaaact gttttgaata ggcgtataga cttgagaatt tgagaatatg tcgtctagca
    26941 gtagagacat attttccggc aatatgaagg aagagacgtc gaaaacgctt gatagtcaag
    27001 gcctttactt catcaccagc tagctgcttt aaaaagaggt agagattata agccagacag
    27061 cccatcatca tacgtacttc attcttaatc atggtcgaac tatctgtctt atccccaaag
    27121 aatcctgctt tcctttcttt gataaagttt tcagcattcc ctcgttcacg atagaattga
    27181 aacagctcct cactatctac actgcttaaa ttggtcaaat gaactttttt acaaccataa
    27241 aaaagagcac ttaatcgtac tcttctcttt taaggattag ttgaattaat tcaaatccac
    27301 tagttctcct gtttctaggt agactaggcg ttcacaaatg ttagcaatgt aatcagcgaa
    27361 gcgttccaga tgccctatga tataaaggta ttgagttcca ttgggaattg aggtttcttg
    27421 gtctttcata agtccaatga tttcctttga taaggcataa taatattggt caatctgttc
    27481 atctttttga gcaatactaa tagcttttga ggcttggtgc aaaggaaagg caaccaataa
    27541 atcggctagc atgctgaggg ataatttacc catttggtgt aactgttctt cgtcaggggc
    27601 tagttgattt tcttttagtt gcaaaacagc tttggcaatg cctgccatat ggtctcccat
    27661 acgttcaagg tctgaacaag aagacatgat gctaatcaca aatcgaaggt cagacacttg
    27721 tggctgctgc aaggccaaca aacgggcaca ggtcaattcg atagcgcttt gaccttggtt
    27781 gatagcatga tccttattga taattagctc tgccatctcc ttgtctttgg aggctaaggc
    27841 cagtaaggct tttgaagctg tttcaaggac aagttgccct agtcctaaaa aggattgttc
    27901 taattcatgc aattctaagt caaattgatt tctcataggt ttttccttcc ttatccgaac
    27961 cgtcctgaaa tatagtcttc tgtgcgctga tcttttggat tggtaaacac gtcaacggta
    28021 tctccaaatt cgcaaatttc tcctgttaag aaaaaagcag ttttatctga aatacgtgaa
    28081 gcttgttgca tgttatgggt aacaatgata atcgtataat ccttttttag ttgctgaatg
    28141 aggtcttcaa tttttaaagt ggagataggg tctaaggctg aagtcggctc atccattaac
    28201 agaatatcag gttctactgc taaagctcgc gcaatgcaaa ggcgttgctg ctgaccgcca
    28261 gataaggaca tagcactctt tttaagatca tctttgactt cttcccaaat ggctgcccct
    28321 tttaaagatt tctccactaa ggcatctaat tgttttttgt ctcgaatacc atgtgtcctt
    28381 gggccataag ccacgttatc atagatagac atggcaaagg gattaggctg ttgaaaaacc
    28441 atccctacac gcttacgtag ctgattaagg ttgaatttgc tactataaat atcttgctca
    28501 tctaagagga cttggccttc aatatggcaa gaaggaacca aatcgttcat ccggttaagg
    28561 gtttttagaa aagttgattt gccacaacca gatgggccta tcaaggcagt aatctgtctt
    28621 tctggtaatt gaatcgaaat attttttaag gcttgaaaat ccccgtaaaa taagtctagg
    28681 tgtctgactg aaaatgttcc catactagga agctcctttc acaagttttc gagataataa
    28741 gcttgataga gtatttatca ttaaaacagt aataatcaaa atcacaccgg tagcataggc
    28801 ttcattgaca tgtagcccct cacttgacag catatacata tgcagggcta gagaacgacc
    28861 tgaagacatg agactacttg gcgtattggt agaggtacct aatgtataca tgagggcagc
    28921 tgtctcacca acgatacggc caatagctag tatcactcca gctaaaatac ctggcatggc
    28981 aactggtaga acaattctaa aaacagtccg taacttacct gccccaagtc cataacttgc
    29041 ttgacgcatg atatcactaa cagataaaag ggcttcttct gttgagcgaa taatgactgg
    29101 caacaccatg atgactgagg ttaagattcc tgataacaga gagtattgaa aacctaagaa
    29161 gactacaaag aagagcatgc caaacagacc aaaaacaatg gaaggaatcc cagacaaggt
    29221 atctgaggcc aatcgcatga ttttaacaca aagggaatct ttttttgtat attccacaag
    29281 ataaaaacca gcaaaaattc ctatgggcaa ggctaaaaga agagcaccaa agaccagaat
    29341 aacggtggaa ataatcgctg gcataaggga aatgttctca gaagtataag tccaagaaaa
    29401 gagggataga cttagatgag gtaagccttt gatgaggata aaaccaatga ttaaaaagag
    29461 agagccaaag gttaaagctg aaaaacaata aacgagaagt tttagcaggt atttactcat
    29521 aagatgattt tcctttcaag taggcaaagt aggcattaat caagagaata aggaaaaaga
    29581 gaactgctga ggttgcaata agggcttccc tatgctgacc tgatgcgtaa gccatttcca
    29641 gaacaatatt ggttgttaag gttctggttc ctgaaaagag tccacttgga ataatcggct
    29701 ggttgcctgc caccaaaata actgccatgg tttcacctac tgcgcgaccg attcctaaaa
    29761 taactgctga taaaatacca gatctcgcag ctggcaagat gacactaaaa atactccgtt
    29821 catgactagc tcctagagcc aagctaccag aataatacgt tttgggaact gttcggatag
    29881 cagattctga caaactgata atggttggca aaatcattat tcctaatagt aacgaagcgg
    29941 ttaggacact catgccattt cctaaaaagc ttctaatcca aggcaccaat aattgtaggc
    30001 cgaaaaaacc ataaacaata gatggaatgg ccgccatcaa gttgatagct gattttaaga
    30061 agccatagac gggctttgga caataataaa ccataaacac cgatgtcaag atgcctgttg
    30121 gcaccccaat cacaatcgct cctaaggtaa ttaataagga accaacgatc attggtaaaa
    30181 taccatagct tgccggaatg ttcgttggcg accaatcact gcctaataaa aaacgggcaa
    30241 agccgtagtt agccatgaaa ggtaagccat tactaaaaat aaagaaacag attagcaaaa
    30301 tagctacaac agctactgtt gcactcatga aaaaaattgc cctaaaaact gcttctttga
    30361 aggcttgttt tatcacatct tgtcctttct agtgaagaaa gtaagggaga tacgacacct
    30421 ccctacttgc cttctttatc ttattgtacg atgaaacgtc tgcatctctt tagagattta
    30481 tggagcaaac attttattta atcttgtccc aggtggttaa tttgccacta aaaacgtctg
    30541 caagttcagc catactgact tggcttgcct tattgtcatt attgaccaca acagcaatac
    30601 cgtctaaagc aatagcatca tgggtgagac tcttaccttc ttcaggagtt aattccctag
    30661 aaaccatacc aatatcagcg gttttctcct taacagcggt aatacctgct gaagacccat
    30721 tagaggtaat atcaatcgta acttctggat tttctttttt ataagcttct gctaattttt
    30781 ccattaaaga agatactgaa gtggaaccta caacagacaa cttgcctgat aagtgttggc
    30841 ttgtatattc tgtggtttca gttttagctt caataaattt attatctgtg accacttgtt
    30901 gaccttgttt ggagtggata aagctgataa aatcttgacc tagcttggaa agattagaag
    30961 accaaacaat gttgaaggga cgttgaagag ggtattcacc atctaaaact gtgtctcgac
    31021 tagccttgac accatcaatc tctaaagcct tgacagattt cgttaaagat cccaaggaga
    31081 tgtagccgat agcattagca ttcccttgaa ctgctgagag aacaccttct gtactatttt
    31141 gaatcacagc tgttttggca gtgttgtcaa tttttttatc accgtctttt ttgagaatcc
    31201 ctgtgatttc tgtgaaggca ccccgtgttc cagagccatt ttctcgtgaa atcacctcaa
    31261 tcgttcctga agctgactgt ttggaagcag ctgactgatt gccacaggca acaagcccaa
    31321 atcctgataa gccaatggct gcaagagtaa gcattttttt gaatttcata ataatcacct
    31381 ttatctctat gtatttttct tgtgtaggct tactacattt atagtctaac aagtctttgt
    31441 aaaggtttat ccctgattca tgtaaagatt gtgtaaagaa tcaaaaaaag ccacttttga
    31501 aaaatggctg ctcctaaaaa tagctttaaa aattattagt cctgtgcgaa agattggtta
    31561 ggaagaaaaa tcgtgaagca actgcctctg ccaagctgac ttgtcaccgt gacttggcca
    31621 cctaataatt gactgagttc tttgacaatg gcaaggccaa gaccagtgcc accagtttgt
    31681 ctgcttcgac ctttattaac tcggtaaaaa cgttcaaaaa tacgatcctg ctctaattga
    31741 ctaataccaa tccctgtatc tgatacagaa atcttaatgc cttcgttcac cttttgggtc
    31801 ttgacctcaa tttttccccc ttgttcagtg taacggatgg cattggataa aagattgagt
    31861 aagatttggg aaagtaattg actatctgat acgagggtga catcatctgg cacctgcacc
    31921 tttagctgta aatccttctt cttgagctga ggttgcaagc tttgagtcaa atcctgtaca
    31981 aattctgcca aagaaagggt cgtccattgt ataggcattt gttgagcctt agataaggta
    32041 agaagatgct caacaatatg ctcaagacgc aagctttctt tgtaaataat gtctagaaag
    32101 tcatccttga gcgcttcttc ttcagcagac atccccttaa tggtttcagc aaagccctta
    32161 atcgaagtaa ctggtgtcct caattcatgg gaggcatttg agacaaaggc taaatttaac
    32221 ttttcataag ttctaatcgt tgttaaatca tatagcaaga cgagcacagc ttccacagat
    32281 tgggtggggc taaaaacggg aactgctgtc acttctaaaa tcaagtcacc ctcatgaaac
    32341 ccacttactt cttgttttaa ccttgttttt tgatcaaagg cttggtgaac taaatttcga
    32401 atatccatcc gtttgaggtc atcaagtgaa cttatgtcgc cgtccacatc gggaaaataa
    32461 tgaggcagag agcgactgga taataacatc tgaccttgag cggaaactaa aaacgtcccc
    32521 atggttaggt gcgacagaag aacctccatt gtttcggcta ggtccttgta ttgctgatcc
    32581 tgttgggaga ctttggtttt taggccagac acatactgag ccaaagactt taagtcttct
    32641 tgcccttttt ctaaaaagta ttcactactg gtcaagagag gttggtgcaa ggtctcaaaa
    32701 gcaacttccc atttccaaag gcaaaagagc cagtagccac cttgtcccaa agaaagggct
    32761 agaagaaaga gaccgatgcc tttactgatc caagttaatg ccatccctgc aatcagaatg
    32821 aggctaacac ttagattgac tagccaaaat tgaaggtagc gtttcatcta taactccttg
    32881 aacttataac cataaccccg aatggttcga ataaattgag gggctttagg attgtcttca
    32941 attttttccc tcaacttacc aatatgaacg tccaccaaac gtgtttcctg cccaaagtca
    33001 tacccccaga tacgttccaa aagacgctct ctagtcagtg tcatgttggg atgtttcata
    33061 agatagagca agagttcaaa ttcttttggg gtcaaactca gtaacttatt cgccttgtag
    33121 acttcatgac gctcagggta tactttcaag gtcccaaata gccaagaatc gtcagcgata
    33181 ttatctgaat catctccttc ttgttctcct ttagttcgcc tgaggacagc cttgacacgc
    33241 gccagcaatt ctctagggct aaaaggcttg gtcaggtagt catcagcccc taattccaag
    33301 gccaaaacct tatcaaattc atcacttttc gcagaaacca tcataattgg agttttgacg
    33361 cctttggctc tcagccgctt acaaacttcc atgccatcta attgtggtaa catgatatca
    33421 agcaagataa aatcaaaggg ttctgtttct gccaaagcta aggccttccg tccatttgtt
    33481 accaattgag tagaaaagcc ttccttactt aaatggtagt caagcaattt cagaatgtgt
    33541 tcttcatcat ccactaataa gacttgtttt gtcatctatt atctcctatt ggtaacatta
    33601 taacacaatt atcagaaatc ctaacattgc taaatcagat taaatttgcc tatcaagact
    33661 agtatctggt caaacgctca atcatctcct tgtgctctgg ataggtcgcc agtagatcta
    33721 ccctttcaaa taattcaaaa tcctcaaatt caaaaccagg agcaacaaga caagaaacca
    33781 gagcatcatc cttatcaact gttgatcccc aaatagtgcc cttaggaaca cagtagtgaa
    33841 gttgttgccc tttggatatg tccaggccta aagtgactgc ttcgtagtga ccatctgctg
    33901 taatcatgtg aacagtaagt ggggatcctg catgaaaata ccagatttca tctgctgtca
    33961 atcggtgaaa atgtgaagga ttcgtttctt ctaataagaa ataaatactg gtataaagcg
    34021 cccttccctt accagcaagg tttatagtgt ctgaagcttt ttttgtttgt ctaaaatagc
    34081 caccttcaac atggggagct aactctagag ttcttatcaa gtcttcttta tccgtcggag
    34141 ccaatgggtt gaagtaactc ttgttcaaag tggttttacg atttcaagaa ctcctctcag
    34201 ttctgaggac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc ctaaaaaaag
    34261 aattagcgaa tgattctggt aaaaagaaat ttcacgctat gaaggctcaa gcgattgtca
    34321 caagtcaagg gagaattgtt tctattgcca tgatatgaag ttgttcaaaa tgagttgcag
    34381 aaatatcgga aaagctggta aaatcttggc tgacagtgat tatcaagggc tcatgaagat
    34441 atatcctcaa gcacaaactc cacgtaaatc cagcaaactc aagccgctaa cagctgaaga
    34501 taaagcctgt aaccatgcgc tatccaagga gagaagcaag gttgagaaca tctttgccaa
    34561 agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac gcttcggatt
    34621 acgaatgaat ttgattgctg gtattatcaa tcatgaacta ggattctagt tttgcaggaa
    34681 gtctattatt tggttaggtg aattagtgaa gtgtttaggc aagtgtctct ggttacgacg
    34741 tcatggcctc taaatcgatt atatttaggg gtcatgacta gtgaagcagt tagctagttc
    34801 gcatataagc ggctagcgtc taacaattag gaactttagt tccaataact ttaagattac
    34861 gacgttttag gacataaatc gatcatattt atgtcctaaa actagtgaag cgcctagcca
    34921 aagtccgaat aggatttggc gttagttact tagattgctt tgcaatcaag taactttggc
    34981 gatttacatc ttctctggcg cttctactcc aagcaagcga agggcttctt tgagaacgac
    35041 tgcggttgcg tagctgaggg ctagacggct gtcgcgttct gggctttcat ccaagatacg
    35101 tgtatgtgca tagtatttgt taaaggattg agccaggcta attgcaaatt tagcaatgat
    35161 agaaggttca aagttatctg ccgcacggtt gataatacgt gggaagtctt gaatgagttt
    35221 aatgatttcc cagctttcag tatcattcaa gctatagttg ccagctgttt ctggtttgaa
    35281 atcggctttg cgtaagatag attggatacg agcgtaggca tattgaacgt aaggtccagt
    35341 ttcaccctcg aaggatacca tagcttctag gtcgaagtcg tatccatttg tacggtcagt
    35401 tttgaggtca tagaatttaa tggctccaac cccaacagca tgtgctactt ggtctttgtt
    35461 ttctagttca ggatttttag cctcgatttg gaccttggca cggctaacag cctctgcaac
    35521 agtaggctct agcaagatga cattcccttt acgagtagag agcttcttcc cttcttttgt
    35581 aaccagacca aaaggaacgt gggtaatgtc gtcactccaa tcgtagccca tctcttgcaa
    35641 gacagctttg agttgtttaa agtgggcaga ttgctcttga ccaacgacgt agatagattt
    35701 agcaaattgg tattcatttt tacggtagag ggctgcagcc aagtcacgtg tgatatagag
    35761 agttgcacca tcagacttct tgatgagggc tggatgttca attccatatt tctcaagatt
    35821 cacaacttgg gcaccttctg attcaagaag tagtcctttt tcagaaagaa tgtctacaac
    35881 tgcatccatc ttatcattgt agaaggcttc tccgttatag ctgtcaaatt caaccttcaa
    35941 ttcattgtaa aggcggttaa attccactaa actttcatcg cggaaccatt gccaaagagc
    36001 gagagcttcc tcatctccat tttcaagttt acggaaccat tcgcgcgctt cttcatccaa
    36061 gctagggtca ttttcagctt cagcgttgat gcggacatag agtttaagga gttcatcgat
    36121 tggatgagct tttacagctt cttcgtcgcc ccattttttg taggcaacaa tcaacatccc
    36181 aaattgttta ccccagtctc ccaaatggtt gaccttgacc gtttgataac cgattttttg
    36241 gaaaatatgt gacaagctat ctccgataac agttgaacgc aggtggccaa tagaaaatgg
    36301 tttagcgata ttcggactag acatgtcgat aacaacattt tcttgtttgc caatattttg
    36361 gtcagcatag tgttcttttt cagtggtaac agcttgcaat acttgagcag aaatggcaga
    36421 tttatcaagg aaaaagttaa cgtaaggtcc tgttgcgaca actttttcaa aggcttggct
    36481 gttcattttt tcagccagtt cagccgcaat catttgtggt gctttacgtt cgacttttgc
    36541 aagagaaaaa gcagggaaag caatgtctcc catttctgag tttttagggg tttccagtaa
    36601 ctttaaaata gcctcttggt ccaggctatc aatgatgcta gataattcgc tagcaatcaa
    36661 ttcttttgta ttcattaaga gctccttttt ggacttttct actattttat cacaatttta
    36721 aagaaagaag aaaaaatttt tgaaatctcc tgtttttttg gtataatatg gttataaata
    36781 tagttataaa tatgcacgca agaggatttt atgagaaaaa gagatcgtca tcagttaata
    36841 aaaaaaatga ttactgagga gaaattaagt acacaaaaag aaattcaaga tcggttggag
    36901 gcgcacaatg tttgtgtgac gcagacaacc ttgtctcgtg atttgcgcga aatcggcttg
    36961 accaaggtca agaaaaatga tatggtgtat tatgtactag taaatgagac agaaaagatt
    37021 gatttggtgg aatttttgtc tcatcattta gaaggtgttg caagagcaga gtttaccttg
    37081 gtgcttcata ccaaattggg agaagcctct gttttggcaa atattgtaga tgtaaacaag
    37141 gatgaatgga ttttaggaac agttgctggt gccaatacct tattggttat ttgtcgagat
    37201 cagcacgttg ccaaactcat ggaagatcgt ttgctagatt tgatgaaaga taagtaaggt
    37261 cttgggagtt gctctcaaga cttatttttg aaaaggagag acagaaaatg gcgatagaaa
    37321 agttatcacc cggcatgcaa cagtatgtgg atattaaaaa gcaatatcca gatgcttttt
    37381 tgctctttcg gatgggtgat ttttatgaat tattttatga ggatgcggtc aatgctgcgc
    37441 agattctgga aatttcctta acgagtcgca acaagaatgc cgacaatccg atccctatgg
    37501 cgggtgttcc ctatcattct gcccaacagt atatcgatgt cttgattgag cagggttata
    37561 aggtggctat cgcagagcag atggaagatc ctaaacaagc agttggggtt gttaaacgag
    37621 aggttgttca ggtcattacg ccagggacag tggtcgatag cagtaagccg gacagtcaga
    37681 ataatttttt ggtttccata gaccgcgaag gcaatcaatt tggcctagct tatatggatt
    37741 tggtgacggg tgacttttat gtgacaggtc ttttggattt cacgctggtt tgtggggaaa
    37801 tccgtaacct caaggctcga gaagtggtgt tgggttatga cttgtctgag gaagaagaac
    37861 aaatcctcag ccgccagatg aatctggtac tctcttatga aaaagaaagc tttgaagacc
    37921 ttcatttatt ggatttgcga ttggcaacgg tggagcaaac ggcatctagt aagctgctcc
    37981 agtatgttca tcggactcag atgagggaat tgaaccacct caaacctgtt atccgctacg
    38041 aaattaagga tttcttgcag atggattatg cgaccaaggc tagtctggat ttggttgaga
    38101 atgctcgctc aggtaagaaa caaggcagtc ttttctggct tttggatgaa accaaaacgg
    38161 ctatggggat gcgtctcttg cgttcttgga ttcatcgccc cttgattgat aaggaacgaa
    38221 tcgtccaacg tcaagaagta gtgcaggtct ttctcgacca tttctttgag cgtagtgact
    38281 tgacagacag tctcaagggt gtttatgaca ttgagcgctt ggctagtcgt gtttcttttg
    38341 gcaaaaccaa tccaaaggat ctcttgcagt tggcgactac cttgtctagt gtgccacgga
    38401 ttcgtgcgat tttagaaggg atggagcaac ctactctagc ctatctcatc gcacaactgg
    38461 atgcaatccc tgagttggag agtttgatta gcgcagcgat tgctcctgaa gctcctcatg
    38521 tgattacaga tgggggaatt atccggactg gatttgatga gactttagac aagtatcgtt
    38581 gcgttctcag agaagggact agctggattg ctgagattga ggctaaggag cgagaaaact
    38641 ctggtatcag cacgctcaag attgactaca ataaaaagga tggctactat tttcatgtga
    38701 ccaattcgca actgggaaat gtgccagccc actttttccg caaggcgacg ctgaaaaact
    38761 cagaacgctt tggaaccgaa gaattagccc gtatcgaggg agatatgctt gaggcgcgtg
    38821 agaagtcagc caacctcgaa tacgaaatat ttatgcgcat tcgtgaagag gtcggcaagt
    38881 acatccagcg tttacaagct ctagcccaag gaattgcgac ggttgatgtc ttacagagtc
    38941 tggcggttgt ggctgaaacc cagcatttga ttcgacctga gtttggtgac gattcacaaa
    39001 ttgatatccg gaaagggcgc catgctgtcg ttgaaaaggt tatgggggct cagacctata
    39061 ttccaaatac gattcagatg gcagaagata ccagtattca actggttaca gggccaaaca
    39121 tgagtgggaa gtctacctat atgcgtcagt tagccatgac ggcggttatg gcccagctgg
    39181 gttcctatgt tcctgctgaa agcgcccatt taccgatttt tgatgcgatt tttacccgta
    39241 tcggagcagc agatgacttg gtttcgggtc agtcaacctt tatggtggag atgatggagg
    39301 ccaataatgc catttcgcat gcgaccaaga actctctcat tctctttgat gaattgggac
    39361 gtggaactgc aacttatgac gggatggctc ttgctcagtc catcatcgaa tatatccatg
    39421 agcacatcgg agctaagacc ctctttgcga cccactacca tgagttgact agtctggagt
    39481 ctagtttaca acacttggtc aatgtccacg tggcaacttt ggagcaggat gggcaggtca
    39541 ccttccttca caagattgaa ccgggaccag ctgataaatc ctacggtatc catgttgcca
    39601 agattgctgg cttgccagca gaccttttag caagggcgga taagattttg actcagctag
    39661 agaatcaagg aacagagagt cctcctccca tgagacaaac tagtgctgtc actgaacaga
    39721 tttcactctt tgatagggca gaagagcatc ctatcctagc agaattagct aaactggatg
    39781 tgtataatat gacacctatg caggttatga atgtcttagt agagttaaaa cagaaactat
    39841 aaaaccaaga ctcactagtt aatctagctg tatcaaggag acttctttga caattctcca
    39901 cttttttgct agaataacat cacacaaaca gaatgaaaag gagctgacgc attgtcgctc
    39961 ccttttgtct attttttaag gagaaagtat gctgattcag aaaataaaaa cctacaagtg
    40021 gcaggccctg gcttcgctcc tgatgacagg cttgatggtt gctagttcac ttctgcaacc
    40081 gcgttatctg caggaagtct taggcgccct ccttactggg aaatatgaag ttatttatag
    40141 tatcggggct tggttgattg gtgtggccgt agtcggtcta gttgctggtg gactcaatgt
    40201 tgtcctcgca gcctatattg cccaaggagt ttcatccgac cttcgggagg atgccttccg
    40261 taaaattcaa accttttctt atgctgatat tgaacaattt aatgcgggaa atctagtcgt
    40321 tcgaatgaca aatgatatca accagattca gaacgttgtc atgatgacct tccaaattct
    40381 tttcagactt cccctcttgt tcatcggttc gtttatccta gcggttcaaa ccttaccttc
    40441 tctgtggtgg gtgattgttc tcatggtagt cttgattttt ggtttgactg ctgtcatgat
    40501 gggaatgatg gggcctcgtt ttgctaaatt tcaaaccctt cttgagcgca tcaatgccat
    40561 tgccaaggaa aatttacgtg gcgttcgtgt ggtcaagtcc tttgtccaag aaaaagagca
    40621 atttgctaag tttacagagg tctcagacga gcttcttggt caaaaccttt acattggtta
    40681 tgccttttca gtagtggaac cctttatgat gttggttggt tacggggcgg tcttcctctc
    40741 tatttggctg gtcgcgggaa tggttcagtc ggatccgtct gttgttggtt ccatcgcttc
    40801 ttttgttaat tacctaagcc agattatctt taccattgtt atggttggat ttttgggaaa
    40861 ttctgtcagc cgtgccatga tttccatgcg tcgtattcga gaaattcttg acgcagagcc
    40921 agctatgacc ttcaaggata tcccagatga agagttggtt ggaagtctta gctttgaaaa
    40981 tgtgaccttt acctatccaa tggacaagga accgatgctg aaagatgtga gctttactat
    41041 tgaacctggt caaatggttg gtgtagttgg agcgactggt gcaggaaagt caaccttggc
    41101 tcaattgatt ccacgtctct ttgatccaca ggacggggcc attaaaatcg gtggcaagga
    41161 tattcgagaa gtgagtgaag gaaccctgcg taaaacagtt tccatcgttc tccaacgtgc
    41221 cattcttttt agtggaacga ttgcagataa cttgagacag gggaagggga atgctactct
    41281 atttgaaatg gagcgcgcag ccaatattgc ccaggctagt gaattcattc atcgtatgga
    41341 gaaaaccttt gaaagtccag ttgaagaacg gggaaccaat ttctctggtg gacaaaaaca
    41401 aaggatgtcg attgcgcgtg ggattgtcag caatccacgt attctgattt ttgacgattc
    41461 gacctcagcc ttggatgcca aatcagagcg cttggtgcaa gaagctttga ataaggactt
    41521 gaaggggacg acaaccatta ttattgctca aaaaattagc tcggttgtcc atgcagacaa
    41581 gatcttggtt ctaaatcaag gacgattgat tggtcaaggc acgcatgcag acttggttgc
    41641 caacaatgcc gtttaccgtg aaatctatga aacacagaaa tgaaagacaa actataagaa
    41701 aagtcaatag ttttatctaa actatttctt atttcaattt gatgatttgg cgatgatttt
    41761 agagcacggc aaaaagccct tgaaaaagtc cattttttca aaggtaatcc tgtgttgatt
    41821 tcagaaatta catcactttt tgttcgtcaa atggcagctc ttttttagga tataaaacag
    41881 ggttcggata agtttttttg caaggtggat gatggctaca ttgtaatgtt ttccttattc
    41941 taacttagtc ttaagatagg ccttaaaagc aggtgaaaag cgagcgcatg ctttggcagc
    42001 ttgtatgagt gcctaccgca gatgagggga accccgtttg atcattcttc cagctaaatc
    42061 aatctgacct gactgataaa tagaagaatc cagtccagcg aaagcttgta attgagcagg
    42121 attatcaaag gcatgaatat tttgaatctc ggctaaaatg accgccccta aacgattccc
    42181 aatcccagta accgtcgtga tgaccgaatt taactcagcc atcaagtcat tgacacattt
    42241 ttccgccttg tcaatgagcc tcttgtaatg tttgatgttt tcattacacg agataaaacg
    42301 tctatgcgtt atcaaactca ttaccaatta aaacaaatgt ggttagatcc tttcggaaat
    42361 tgtcaagcga ttggaggaaa tgaactaatc cacagcggct tattccaagt ataccacttg
    42421 ggctttggca gtagctaact gcgctaaata taatataagg aggagtaaaa tgaagacagt
    42481 tcaatttttt tggcattatt ttaaggtcta caagttctca tttgtagttg tcatcctgat
    42541 gattgttctg gcgacttttg cccaagccct ctttccagtc ttttctggac aagcggtgac
    42601 gcagctagcc aatttagttc aagcttatca aaatggcaat ccagaacttg tatggcaaag
    42661 cctatcagga atcatggtca atcttggcct gctggttttg gttctattta tctctagtgt
    42721 aatatacatg tgtctcatga cgcgcgtgat tgcagaatcg accaacgaga tgcgcaaagg
    42781 tctctttggt aagcttgctc agttgacggt ttctttcttt gaccgtcgac aagatggcga
    42841 tatcctgtct cattttacca gtgatttgga taatatcctc caagccttta acgaaagctt
    42901 gattcaggtc atgagcaata ttgttttata cattggtctg attcttgtca tgttttcgag
    42961 aaatgtgacg ctggctctca tcaccattgc cagcacccca ttggctttcc ttatgctgat
    43021 tttcatcgtg aaaatggcac gcaaatacac caacctccag cagaaagagg tagggaagct
    43081 caacgcctat atggatgaga gcatctcagg ccaaaaagcc gtgattgtgc aaggaattca
    43141 agaggatatg atggcaggat ttcttgaaca aaatgagcgc gtgcgcaagg caacctttaa
    43201 aggaagaatg ttctcaggaa ttcttttccc tgtcatgaat gggatgagcc tgattaatac
    43261 agccatcgtc atctttgctg gttcggctgt acttttgaat gataagtcta ttgaaacaag
    43321 tacagcccta ggtttgattg ttatgtttgc acaattttca cagcagtact accagcctat
    43381 tatccaagtt gcagcgagtt ggggaagcct tcagttggcc tttactggag ctgaacgaat
    43441 tcaggaaatg tttgatgcag aggaggaaat ccgacctgaa aaggctccaa ccttcactaa
    43501 gttgcaagaa agtgttgaaa tcagtcatat cgatttttca tacttgcctg ataaacctat
    43561 tttgaaagat gtcagcattt ctgcccctaa aggccagatg acagcagttg ttgggccgac
    43621 aggttcagga aaaacgacta ttatgaacct catcaatcgc ttttatgatg ttgatgctgg
    43681 tggtatttat tttgatggta aagacattcg tggctatgac ttagatagtc ttagaagcaa
    43741 ggtgggaatt gtattgcaag attcggtctt gtttagcgga acgattcgag acaatatccg
    43801 atttggtgtg ccagatgcta gtcaggaaat ggttgaggta gcagcaaaag caacccacat
    43861 tcacgactat atcgaaagtt tgcctgataa gtacgatact cttattgatg atgaccagag
    43921 catcttttca acagggcaga agcaattgat ttcaatcgct cgaaccctga tgacagatcc
    43981 agaagttctc attctcgatg aagcaacttc aaacgtagat acggtgacag aaaacaagat
    44041 tcagcatgcc atggaggtgg ttgtagcagg tagaactagt ttcgtcattg cccaccgctt
    44101 gaaaaccatt ctcaatgcag atcagattat tgtccttaaa gatggagaag tcattgaacg
    44161 tggtaaccac catgaacttt tgaagctagg tggcttttat tcagaactct atcacaatca
    44221 atttgttttc gaataagaaa gaagttgtcc tatgtgggca gctttttctt gtccataaaa
    44281 aatgtttatc acagccttaa aaaaaacata ttagacgaaa gtcattttga gtgatatgat
    44341 aggactatcg ttagcattcg aaaggagagg catcatggct agaacggttg taggagttgc
    44401 tgcaaatcta tgtcccgtag acgcagaagg caaaatcatt cattcatctg tatcttgtag
    44461 attcgcagag atcattcgtc aaatcggtgg tctcccttta gtcattcctg ttggtgatga
    44521 gtcagttgta cgtgattatg tggaaatgat tgacaaactc attttgacag gaggccaaaa
    44581 tgttcatcct cagttttatg gagagaaaaa gaccgtcgag agcgatgatt acaatctggt
    44641 ccgtgacgaa tttgaattgg cactcttgaa ggaagcgctt cgtcagaata aaccaattat
    44701 ggcaatctgt cgcggtgtcc aacttgtcaa tgttgccttt ggtggaaccc tcaatcaaga
    44761 aatcgaaggt cactggcaag gactaccttt cgggacatct cactctattg agacagtaga
    44821 aggaagcgtg gtggctaagc tatttgggaa agaaagtcag gtcaactcag tccatcgtca
    44881 aagcattaaa gatttggcac ctaatttccg tgtaactgct attgattcaa gagaccaaac
    44941 tattgaagcg attgagtcta tcgacgagca ccgcattatc ggtttacagt ggcatccaga
    45001 gtttctggtt aatgaagaag atggcaattt agaattattt gagtatttat tgaatgaact
    45061 gtaacgattg gaacatctgg tcgttctttc ttttattttt tcaaaatttt tagaatgtgg
    45121 cattttacgc aaacgtttga attctgataa aaattggaga aattcgacaa aaaaacttga
    45181 aaaaaacgaa ggtaagcgtt atgatagaaa agaagaaata ttggaggaag aacatgtcac
    45241 atattaaatt tgattattca aaagttttag acaaatttgt tgcaccacat gaagtggaat
    45301 acatgcaatc acaagtaaca gcagcagatg aattgatccg taaaggaact ggtgctggta
    45361 gcgacttctt gggatggttg gaccttcctg aaaaatacga tcgcgaagaa tttgaccgca
    45421 tcttgaaagc tgctgagcaa atcaagtcag acagcgatgt tttggttgtg atcggtattg
    45481 gtggatctta ccttggtgca aaagcagcaa tcgacttctt gaatcaccat tttgctaact
    45541 tgcaaacaaa agaagaacgc aaagctccac aaatccttta cgcaggaaac tcaatctcat
    45601 ctacttacct tgctgacttg gtagagtatg ttgcagacaa agacttctca gtaaacgtaa
    45661 tttctaaatc aggtacaaca actgaaccag cgattgcttt ccgtgtcttt aaagaactct
    45721 tggttaagaa atacggtcaa gaagaagcta acaaacgtat ctatgcaaca actgaccgcc
    45781 aaaagggtgc tgttaaggtt gaagcagacg ctaacggttg gggaacattt gttgttccag
    45841 atgatatcgg tggacgcttc tcagtattga cagccgttgg tttgcttcca atcgcagcat
    45901 caggagctga catcaaagct cttatggaag gtgcgaatgc agctcgcaaa gactacactt
    45961 cagacaaaat ctctgaaaac gaagcttacc aatacgcagc tgttcggaac atcctttatc
    46021 gtaaaggcta tgcaactgag atcttggtaa actatgagcc atcacttcaa tacttctcag
    46081 aatggtggaa acaattggct ggtgaatcag aaggaaaaga ccaaaaaggt atctacccaa
    46141 cttcagccaa cttctcaact gacttgcact cacttggtca atttatccaa gaaggaactc
    46201 gtatcatgtt tgaaacagtt gtccgtgttg acaaacctcg taaaaacgtg cttattccta
    46261 ctttggaaga agaccttgac ggacttggtt accttcaagg aaaagacgtt gactttgtaa
    46321 acaaaaaagc aactgacggt gttcttcttg cccacacaga tggtgatgta ccaaacatgt
    46381 atgtgactct tccagagcaa gacgctttca ctcttggtta cactatctac ttcttcgaat
    46441 tggcaattgc cctttcaggt tacttgaatg ctatcaaccc atttgaccaa ccaggtgttg
    46501 aagcttataa acgtaacatg tttgcccttc ttggaaaacc aggatttgaa gaattgagca
    46561 aagaacttaa cgcacgtcta taatagaaga aaagagtggt ttgcccactc tttttactct
    46621 ctttatccat agaaattgga ctcagccaag acttgtgata taatatagaa agcaaaaagg
    46681 cagacgccta gataatagga gaaactatgt caaaagatat ccgcgtacgt tacgcaccaa
    46741 gtccaacagg actactacac atcggaaatg ctcgtacagc attgtttaat tacttgtatt
    46801 cgcgccatca tggtggaaca tttctcatcc gtatcgaaga tactgaccgt aaacgccatg
    46861 tcgaggatgg tgaacgttca caacttgaaa accttcgctg gttaggcatg gattgggatg
    46921 aaagtccaga atcacatgag aattatcgcc agtctgagcg tttggacttg tatcaaaaat
    46981 acattgacca actattagct gaaggaaaag cctataaatc ttacgttaca gaagaagagt
    47041 tggcagctga acgcgaacgc caagaagtag ctggcgaaac accacgctac atcaatgaat
    47101 accttggtat gagtgaagaa gaaaaagcag cttacatcgc agaacgtgaa gcagcaggga
    47161 tcatcccaac tgttcgtttg gctgtcaatg agtcaggtat ctacaagtgg catgatatgg
    47221 tcaaaggcga tatcgaattt gaaggtggca atatcggtgg tgactggatt atccaaaaga
    47281 aagacggtta cccaacttac aactttgccg ttgccatcga tgaccatgat atgcaaatct
    47341 ctcatgttat ccgtggagac gaccatattg ctaacacacc aaaacagctc atggtttatg
    47401 aagcacttgg ttgggaagct ccagagtttg gtcacatgac cttgattatc aactctgaga
    47461 ctggtaagaa gttatctaaa cgtgatacta atacacttca atttatcgaa gattaccgaa
    47521 agaaaggtta ccttccagaa gcagtcttta actttattgc tcttcttggt tggaacccag
    47581 gtggtgaaga tgagattttc tctcgtgaag aactcattaa actttttgat gaaaaccgcc
    47641 tcagcaagtc accagcagcc tttgatcaga aaaaactaga ctggatgagc aatgattata
    47701 tcaagaatgc agaccttgaa accatctttg aaatggcaaa accattctta gaggaagcag
    47761 gccgtttgac tgacaaggct gaaaaattag ttgagctcta taaaccacaa atgaaatcag
    47821 tagatgagat tatcccattg acagatcttt tcttctcaga tttcccagaa ttgacagaag
    47881 cagagcgcga agtcatgacg ggtgaaacag ttccaacagt tcttgaagca ttcaaagcaa
    47941 aacttgaagc gatgacagat gataaatttg tgacagaaaa tatcttccca caaattaaag
    48001 cagttcaaaa agaaacaggt attaaaggga aaaatctttt catgcctatt cgtatcgcag
    48061 tttcaggcga aatgcatggg ccagaattac cagatacaat tttcttgctt ggacgtgaaa
    48121 aatcaattca gcatatcgaa aacatgctaa aagaaatctc taaataaaga ggatacaata
    48181 atggacatct gggaaaagat gtacgaagaa gcacagaaac tatacaatcc acatgaagta
    48241 tcagactttg tttatgccaa tcatgttgta gctgcagtag aagcagaaga tggacaaata
    48301 tttacaggat tctgtatgga gggaacctgt ggtgttttcc atctctgcgc agaacgggcg
    48361 gccctcttca atatgtacca attttcagga caaactaagg ttaagaaagt attagccttt
    48421 cgagacaaac caccttatgg tggaagttca gccatgcctt gcggtgcttg tagagaattc
    48481 cttttagagc ttaatgctga aaataaagat gcagaattca tgatggacta taatataaga
    48541 aaaacagtta aagtcgcaga actaatccct tattggtggg gagaagaacg tgcttctaag
    48601 tttaatgagc gatagaagag tcagtataat tctggctttt tttactataa gttgaaaaaa
    48661 t
//
LOCUS       DAVLXF010000004        43174 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLXF010000004 DAVLXF010000000
VERSION     DAVLXF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43174
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.004"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..436
                     /locus_tag="VYO12_000232"
     CDS             <1..436
                     /locus_tag="VYO12_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1586900.1"
                     /translation="LEADNQEQLFDQVATLLEEREIVTPTYREALITREKSFPTGLDM
                     EFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNS
                     SSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAETTA"
     gene            474..779
                     /locus_tag="VYO12_000233"
     CDS             474..779
                     /locus_tag="VYO12_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1586901.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            827..2302
                     /locus_tag="VYO12_000234"
     CDS             827..2302
                     /locus_tag="VYO12_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1586902.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2328..2399
                     /locus_tag="VYO12_000235"
                     /pseudo
     CDS             2328..2399
                     /locus_tag="VYO12_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2697..9383
                     /locus_tag="VYO12_000236"
     CDS             2697..9383
                     /locus_tag="VYO12_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1586903.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTANVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPSKLVLERYVGPGFEVPTYYSNYQAYESGHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGKERIFT
                     VINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEADQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9631..10485)
                     /locus_tag="VYO12_000237"
                     /pseudo
     CDS             complement(9631..10485)
                     /locus_tag="VYO12_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10697..11056)
                     /locus_tag="VYO12_000238"
     CDS             complement(10697..11056)
                     /locus_tag="VYO12_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1586904.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKLSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            11148..12104
                     /locus_tag="VYO12_000239"
                     /pseudo
     CDS             11148..12104
                     /locus_tag="VYO12_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12088..12645
                     /locus_tag="VYO12_000240"
     CDS             12088..12645
                     /locus_tag="VYO12_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1586905.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12638..12892
                     /locus_tag="VYO12_000241"
     CDS             12638..12892
                     /locus_tag="VYO12_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586906.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12904..14958
                     /locus_tag="VYO12_000242"
     CDS             12904..14958
                     /locus_tag="VYO12_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1586907.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLILPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15137..15997
                     /locus_tag="VYO12_000243"
     CDS             15137..15997
                     /locus_tag="VYO12_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1586908.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSINFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQFLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGVLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDQVFKKD"
     gene            16208..16918
                     /gene="ccdA2"
                     /locus_tag="VYO12_000244"
     CDS             16208..16918
                     /gene="ccdA2"
                     /locus_tag="VYO12_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1586909.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            16938..17504
                     /locus_tag="VYO12_000245"
     CDS             16938..17504
                     /locus_tag="VYO12_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1586910.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17515..18627
                     /gene="msrB"
                     /locus_tag="VYO12_000246"
     CDS             17515..18627
                     /gene="msrB"
                     /locus_tag="VYO12_000246"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1586911.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18895..19632
                     /locus_tag="VYO12_000247"
     CDS             18895..19632
                     /locus_tag="VYO12_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1586912.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19629..21320
                     /locus_tag="VYO12_000248"
     CDS             19629..21320
                     /locus_tag="VYO12_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1586913.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            21436..21999
                     /locus_tag="VYO12_000249"
     CDS             21436..21999
                     /locus_tag="VYO12_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1586914.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALTSRPKLIGGARAHYPQDAIRKF"
     gene            22021..27732
                     /locus_tag="VYO12_000250"
     CDS             22021..27732
                     /locus_tag="VYO12_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1586915.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGTMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPRKDEQAPVEPENQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TVNQSIEQPKVETPAVEKQTEPTEEPKVEQAGEPVAPREDEQAPTAPVEPEKQPEVPE
                     EEKAVEETPKPEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLET
                     AKGVYASEPVKQPEVNSETNKLKTAIDALNVDKTELNNTIADAKTKVKEHYSDRSWQN
                     LQTEVTKAEKVAANTDAKQSEVNEAVEKLTATIEKLVELSEKPILTLTSTDKKILERE
                     AVAKYTLENQNKTKIKSITAELKKGEEVINTVVLTDDKVTTETISAAFKNLEYYKEYT
                     LSTTMIYDRGNGEETETLENQNIQLDLKKVELKNIKRTDLIKYENGKETNESLITTIP
                     DDKSNYYLKITSNNQKTTLLAVKNIEETTVNGTHVYKVTAIADNLVSRTADNKFEEEY
                     VHYIEKPKVHEDNVYYNFKELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYITKEFTG
                     KLLSSEGKQFAITELEHPLFDKIKAGTVKNINFENIDINVSNQDNIATIANEINGTTR
                     IENIKATGTITARNNVAGFVNNIKENAALENVAFIGNINSVGNNSTVGGIAGSNYMGF
                     VNKAYVDANITAANASASMLVPLVTYMLNSYQYGTKSKVTNSVAKGTLDVKNTRFVGG
                     VVAKTWPWGVIKDIVTYAKVIKGEEVYGSNEINDGDARQFLSDIYRVNGASSGANNFT
                     NLQKIKQLTKEQADAKVATFNITADKLESELSPLAKLNEEKAYSSIQDYNAEYNQAYK
                     NLEKLIPFYNKDYIVYQGNKLNKEHPLNTKEVLSVTAMNNNEFITNLDEANKIIVHYA
                     DGTKDYFNLSSSSEGLSNVKEYTITDLGIKYTPNIVQKDNTTLVNDIKSILEPVDLQS
                     QTMYQHLNRLGDYRVNAIKDLYLEESFTAVKKNLTNLITKLVQNEEHQLNDSPAARQM
                     LRDKVEKNKAALLLGLTYLNRYYGVKFGDVNIKELMLFKPDFYGEKVSVLDRLIEIGS
                     KENNIKGSRTFDAFGEVLAKYTKSGNLDVFLNYNRQLFTNIDNMNDWFIDATKDHVYI
                     AERASEVEEIKNAKYRAYANLNVAQHRKMILPLLNITKSHMFIASNYNAIAIGSAEKL
                     GKKSLEDIKASVDYAADGYRNYYDFWYRLVPDNVKQYLLRDTVIPIWEGYNAPGGWVE
                     KYGRYNTDNVYTPLREFFGPIEKYYGYNGTGAYAALYDNPMDPRSDVSYVALNMIGDY
                     GISVYTHETTHINDRKIYLGGFGRREGTDAEAFAQGMLQLPVPGSGFNEYGSLGLNTV
                     FKKPNDGNQWYDTDPKSLTTRDDIDKYMRGYNDALMLVDHLEADAVINQNNKELNGAW
                     FKKIDKKFRDASQLNQWDEIRNLTTEEKDNLNITSIDSLIDQQLMTNRQIGNRVYKPE
                     VVSQYDQSPYVGVEMTAGIYGGNTSKGAPGAVSFKHNAFRLWGYYGYLNGFLGYTSNK
                     YKQQSTKDGESVLSDEYIIKKISNNTFNTIEEFKKAYFKEVKDKATKGLTTFEVNGSS
                     VSSYDDLLTLFKEAVKKDAETLKQEANGHKTVSMTNTVKLKEAVYKKLLQQTDSFKTS
                     IFR"
     gene            27793..29514
                     /gene="pabB"
                     /locus_tag="VYO12_000251"
     CDS             27793..29514
                     /gene="pabB"
                     /locus_tag="VYO12_000251"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1586916.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            29515..30132
                     /locus_tag="VYO12_000252"
     CDS             29515..30132
                     /locus_tag="VYO12_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1586917.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            30182..31180
                     /locus_tag="VYO12_000253"
     CDS             30182..31180
                     /locus_tag="VYO12_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1586918.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLTSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTV
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            31307..32266
                     /locus_tag="VYO12_000254"
     CDS             31307..32266
                     /locus_tag="VYO12_000254"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1586919.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTVGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            32363..33202
                     /locus_tag="VYO12_000255"
     CDS             32363..33202
                     /locus_tag="VYO12_000255"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1586920.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRIDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(33250..33420)
                     /locus_tag="VYO12_000256"
     CDS             complement(33250..33420)
                     /locus_tag="VYO12_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1586921.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            33541..34425
                     /gene="miaA"
                     /locus_tag="VYO12_000257"
     CDS             33541..34425
                     /gene="miaA"
                     /locus_tag="VYO12_000257"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1586922.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHFVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            34418..35656
                     /gene="hflX"
                     /locus_tag="VYO12_000258"
     CDS             34418..35656
                     /gene="hflX"
                     /locus_tag="VYO12_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1586923.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKFQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDS
                     EVITGYISEKNKWRLEEFYD"
     gene            35649..36272
                     /locus_tag="VYO12_000259"
     CDS             35649..36272
                     /locus_tag="VYO12_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1586924.1"
                     /translation="MTDIKALALKYGGYTSLDKVYLVQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     DSDGLGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            36287..37216
                     /locus_tag="VYO12_000260"
     CDS             36287..37216
                     /locus_tag="VYO12_000260"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1586925.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTVRPRKISKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTNASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            37237..37992
                     /locus_tag="VYO12_000261"
     CDS             37237..37992
                     /locus_tag="VYO12_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1586926.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(38046..38954)
                     /locus_tag="VYO12_000262"
     CDS             complement(38046..38954)
                     /locus_tag="VYO12_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1586927.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLSHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            39018..39287
                     /locus_tag="VYO12_000263"
     CDS             39018..39287
                     /locus_tag="VYO12_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1586928.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            39287..39667
                     /locus_tag="VYO12_000264"
     CDS             39287..39667
                     /locus_tag="VYO12_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1586929.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILRARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            39756..39983
                     /locus_tag="VYO12_000265"
     CDS             39756..39983
                     /locus_tag="VYO12_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586930.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLEYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(40023..40748)
                     /locus_tag="VYO12_000266"
     CDS             complement(40023..40748)
                     /locus_tag="VYO12_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV1586931.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            40910..42751
                     /gene="typA"
                     /locus_tag="VYO12_000267"
     CDS             40910..42751
                     /gene="typA"
                     /locus_tag="VYO12_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1586932.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            42771..43025
                     /locus_tag="VYO12_000268"
     CDS             42771..43025
                     /locus_tag="VYO12_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1586933.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT        13362 a         8044 c         9460 g        12308 t
ORIGIN      
        1 cttagaagcg gataatcaag aacaactctt tgatcaggtt gcaactttat tggaagaacg
       61 agaaattgtc actccaactt atcgtgaagc cttgatcacg cgtgaaaagt catttccaac
      121 tggtttagat atggaatttc taggaaagga cttgccaaat gtagcgattc ctcatacaga
      181 tattgttcat aatctagctg agaaagtggt ggttgttcga ttagaaaaac cagtaacttt
      241 tcacaatatg atagctcctg ataaggaagt agaagtatcc ttgctcttct ttatcatcaa
      301 taactcaagt tcaagtcaaa caaatattct ggctcagttg atggactttt tcacaggaaa
      361 tggacatctt gaagacctat caaaaatttc cgaaccagaa aaactttatg cttacattgc
      421 tgaaacaacc gcttaatctt gtctattaaa aaataaaatc ggaggaaatc taaatgatta
      481 aaattcttgc tgcctgcggt gcaggtgtta actcaagtca ccaaattaaa agtgctctag
      541 aagaagaact ttcaaaccgt ggttatgatg ttcactgtga tgcagtcatg gtgaaagatg
      601 taaacgaaga ccttatgaaa ggttacgata tctttacacc aatcgctgca acagaccttg
      661 gttttgaacc aggtatccca gttatcgaag ctggaccaat cttattccgt atcccagcaa
      721 tgagcgctcc agtatttgac aatattgaag cagctattaa agaacatgga ttaagctaat
      781 tctaatttgt taacattcat ataacataaa aaagggagga actgttatgg atgcaatctt
      841 tgacctaatc ggaaaggttt tcaatcccat cttagaaatg ggtggacctg tcatcatgtt
      901 gatcattttg acagtattgg ctttactttt tggagtgaaa ttctccaaag cgcttgaagg
      961 tggtatcaaa cttgccatcg ctcttacagg tatcggtgct atcatcggta tgctaaatgg
     1021 tgctttctca gcatcacttg caaaattcgt tgaaaacact ggtatccaat tgagtattac
     1081 agacgttggt tgggcaccac tcgcaacaat cacttggggt tctgcttgga cactatactt
     1141 cttgctcatc atgttgattg tcaacgtagt gatgcttgct atgaagaaaa ccgatacact
     1201 tgatgtcgat atctttgata tctggcactt gtctatcaca ggtctcttga ttaaatggta
     1261 tgctgataac aatggtgtga gtcaaggggt ttcactcttt attgctacag cagctatcgt
     1321 ccttgtcggt gtgttgaaaa ttatcaactc tgacttgatg aaacctacat ttgatgacct
     1381 tcttaacgcc ccaagttcat caccaatgac atcaactcac atgaactaca tgatgaaccc
     1441 agttatcatg gttttggata agatttttga aaaattcttc ccaggccttg ataaatatga
     1501 ctttgatgct gctaaattga acaagaaaat cggtttctgg ggatctaaat tcttcatcgg
     1561 tttcatcctt ggtatcgtta tcggtattat gggaactcca catccaattg caggtgttgc
     1621 agatgcagat aaatggcgtc ttgttatcaa aggatggttg tctcttggtt tgactgccgg
     1681 tgtatctttg gaactcttct cacttatcgg ttcatggttc atcgcagccg tagaaccact
     1741 atcacaaggt attacaaacg ttgctactaa acgtcttcaa ggacgtaaat tcaatatcgg
     1801 tcttgactgg ccattcatcg ctggtcgtgc tgaaatctgg gcttgtgcca acgtacttgc
     1861 accaatcatg ttgattgaag cagtgcttct ttcaaaagtt ggaaatggta tcttgccact
     1921 tgcaggtatc atcgctatgg gtgttactcc agctctcttg gttgtaactc gtggtaaatt
     1981 gctccgtatg attatcttcg gaacactctt gttgccactc ttccttcttt caggtacact
     2041 tattgcacca tttgcaacag aacttgctaa aggtgtaggt gccttcccag aaggtgtgag
     2101 ccaaactcaa ttgattactc actctactct tgaaggacca atcgaaaaac ttcttggttg
     2161 gacaattggt aacactacaa ctggtgatat caaagcaatc cttggtgcag tagtcttcct
     2221 tgtattctat atcggtatct ttgcttggta cagaaaacaa atgatcaaac gtaacgaaga
     2281 gtacgcagca aaagcaaaat aatgcgctcc tataaaatat aaacttaatg aaaatcaaag
     2341 agcaaactag gaagctagcc gcaggttgct caaaacacag ttttgaggtt gtagataaga
     2401 ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagct
     2461 caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt cagctcaaaa catggttttg
     2521 aggttgtaga tgaaactgac gaagtcagta atcatatcta cggcaaggcg acactgacga
     2581 agtttgaatt ggattttcgc agagtgtaaa ttgttgaaac taggttgtca taccatgtat
     2641 accacttggg ctttggtagt agctaactgc actaaatata atataaggag ggttttatgg
     2701 ggaaaggcca ttggaatcgg aaaagagttt atagcattcg taagtttgct gtgggagctt
     2761 gctcagtaat gattgggact tgtgcagttc tattaggagg aaatatagct ggagaatctg
     2821 tagtttatgc ggatgaaaca cttattactc atactgctga gaaacctaaa gaggaaaaaa
     2881 tgatagtaga agaaaaggct gataaagctt tggaaactaa aaatgtagtt gaaaggacag
     2941 aacaaagtga acctagttca actgaggcta ttgcatctga gaagaaagaa gatgaagccg
     3001 taactccaaa agaggaaaaa gtgtctgcta aaccggaaga aaaagctcca aggatagaat
     3061 cacaagcttc aagtcaagaa aaaccgctca aggaagatgc taaagctgta acaaatgaag
     3121 aagtgaatca aatgattgaa gacaggaaag tggattttaa tcaaaattgg tactttaaac
     3181 tcaatgcaaa ttctaaggaa gccattaaac ctgatgcaga cgtatctacg tggaaaaaat
     3241 tagatttacc gtatgactgg agtatcttta acgatttcga tcatgaatct cctgcacaaa
     3301 atgaaggtgg acagctcaac ggtggggaag cttggtatcg caagactttc aaactagatg
     3361 aaaaagacct caagaaaaat gttcgcctta cttttgatgg cgtctacatg gattctcaag
     3421 tttatgtcaa tggtcagtta gtggggcatt atccaaatgg ttataaccag ttctcatacg
     3481 atatcaccaa ataccttcat aaagatggtc gtgagaatgt gattgctgtc catgcagtca
     3541 acaaacagcc aagtagccgt tggtattcag gaagtggtat ctatcgtgat gtgactttac
     3601 aagtgacaga taaggtgcat gttgagaaaa atgggacaac tattttaaca ccaaaacttg
     3661 aagaacaaca acatggcaag gttgaaactc atgtgaccag caaaatcgtc aatacggacg
     3721 acaaagacca tgaacttgta gccgaatatc aaatcgttga acgaggtggt catgctgtaa
     3781 caggcttagt tcgtacagcg agtcgtacct taaaagcaca tgaatcaaca agcctagatg
     3841 cgattttaga agttgaaaga ccaaaactct ggaccgtttt aaatgacaaa cctgccttgt
     3901 acgaattgat tacgcgtgtt taccgtgacg gtcaattggt tgatgctaag aaggatttgt
     3961 ttggttaccg ttactatcac tggactccaa atgaaggttt ctctttgaat ggtgaacgta
     4021 ttaaattcca tggagtatcc ttgcaccacg accatggggc gcttggagca gaagaaaact
     4081 ataaagcaga atatcgccgt ctcaaacaaa tgaaggagat gggagttaac tccatccgta
     4141 caacccacaa ccctgctagt gagcaaacct tgcaaatcgc agcagaacta ggtttactcg
     4201 ttcaggaaga ggcctttgat acttggtatg gtggcaagaa accttatgac tatggacgtt
     4261 tctttgaaaa agatgccact cacccagaag ctcgaaaagg tgaaaaatgg tctgattttg
     4321 acctacgtac catggtcgaa agaggcaaaa ataaccctgc tatcttcatg tggtcaattg
     4381 gtaatgaaat aggtgaagct aatggtgatg cccactcttt agcaactgtt aaacgtttgg
     4441 tcaaggttat caaggatgtt gataagactc gctatgttac catgggagca gataaattcc
     4501 gtttcggtaa tggtagcgga gggcatgaga aaattgctga tgaactcgat gctgttggat
     4561 ttaactattc tgaagataat tacaaagccc ttagagctaa gcatccaaaa tggttgattt
     4621 atggatcaga aacatcttca gctacccgta cacgtggaag ttactatcgc cctgaacgtg
     4681 aattgaaaca tagcaatgga cctgagcgta attatgaaca gtctgactat gggaatgatc
     4741 gtgttggttg ggggaaaaca gcaaccgctt catggacttt tgaccgtgac aacgctggct
     4801 atgctggaca gtttatctgg acaggtacgg actatattgg tgaacctaca ccatggcaca
     4861 accaaaatca aactcctgtt aagagctctt actttggtat cgtagataca gccggcattc
     4921 caaaacatga cttctacctc taccaaagcc aatgggtttc tgttaagaag aaaccgatgg
     4981 tacaccttct tcctcactgg aactgggaaa acaaagaatt agcatccaaa gtagctgact
     5041 cagaaggtaa gattccagtt cgtgcttatt cgaatgcttc tagtgtagaa ttgttcttga
     5101 atggaaaatc tcttggtctt aagactttca ataaaaaaca aaccagcgat gggcggactt
     5161 accaagaagg tgcaaatgct aatgaacttt atcttgaatg gaaagttgcc tatcaaccag
     5221 gtaccttgga agcaattgct cgtgatgaat ctggcaagga aattgctcga gataagatta
     5281 cgactgctgg taagccagcg gcagttcgtc ttattaagga agaccatgcg attgcagcag
     5341 atggaaaaga cttgacttac atctactatg aaattgttga cagccagggg aatgtggttc
     5401 caactgctaa taatctggtt cgcttccaat tgcatggcca aggtcaactg gtcggtgtag
     5461 ataacggaga acaagccagc cgtgaacgct ataaggcgca agcagatggt tcttggattc
     5521 gtaaagcatt taatggtaaa ggtgttgcca ttgtcaaatc aactgaacaa gcagggaaat
     5581 tcacactgac agcccattca gatctcttga aatctagtca agtcactgtc tttactggta
     5641 aaaaagaagg acaagagaag actgttttgg ggacagaagt gccaaaagta cagaccatta
     5701 ttggagaggc acctgaaatg cctaccactg ttccgtttgt atacagtgat ggtagccgtg
     5761 cagaacgtcc tgtaacctgg tcttcagtag atgtgagcaa gcctggtatt gtaacggtga
     5821 aaggtatggc tgacggacga gaagtagaag ctcgtgtaga agtgattgct cttaaatcag
     5881 agctaccagt tgtgaaacgt attgctccaa atactgactt gaattctgta gacaaatctg
     5941 tttcctatgt tttgactgat ggaagtgttg aagagtatga agtggacaag tgggagattg
     6001 ccgaagaaga taaagctaag ttagcaattc caggttctcg tattcaagcg accggttatt
     6061 tagaaggtca accaattcat gcaacccttg tggtagaaga aggcaatcct gcggcacctg
     6121 cagtaccaac tgtaacggtt ggtggtgaag ctgtcacagg tcttactagt caaaaaccaa
     6181 tgcaataccg cactcttgct tatggagcta agttgccaga agtcacagca agtgctaaaa
     6241 atgcagctgt tacagttctt caagcaagcg cagcaaacgg catgcgtgcg agcatcttta
     6301 ttcagcctaa agatggtggc cctcttcaaa cctatgcaat tcaattcctt gaagaagcgc
     6361 caaaaattgc tcacttgagc ttgcaagtgg aaaaagctga cagtctcaaa gaagaccaaa
     6421 ctgtcaaatt gtcggttcga gctcactatc aagatggaac gcaagctgta ttaccagctg
     6481 ataaagtaac cttctctaca agtggtgaag gggaagtcgc aattcgtaaa ggaatgcttg
     6541 agttgcataa gccaggagca gtcactctga acgctgaata tgagggagct aaaggccaag
     6601 ttgaactcac tatccaagcc aatactgaga agaagattgc gcaatccatc cgtcctgtaa
     6661 atgtagtgac agatttgcat caggaaccaa gtcttccagc aacagtaaca gttgagtatg
     6721 acaaaggttt ccctaaaact cataaagtca cttggcaagc tattccgaaa gaaaaactag
     6781 actcctatca aacatttgaa gtactaggta aagttgaagg aattgacctt gaagcgcgtg
     6841 caaaagtctc tgtagaaggt atcgtttcag ttgaagaagt cagtgtgaca actccaatcg
     6901 cagaagcacc acaattacca gaaagtgttc ggacatatga ttcaaatggt cacgtttcat
     6961 cagctaaggt tgcatgggat gcgattcgtc cagagcaata cgctaaggaa ggtgtcttta
     7021 cagttaatgg tcgcttagaa ggtactcaat taacaactaa acttcatgtt cgcgtatctg
     7081 ctcaaactga gcaaggtgca aacatttctg accaatggac cggttcagaa ttgccacttg
     7141 cctttgcttc agattcaaat ccaagcgacc cagtttcaaa tgttaatgac aagctcattt
     7201 cctacaataa ccaaccagcc aatcgttgga caaactggaa tcgtagtaat ccagaagctt
     7261 cagtcggtgt tctgtttgga gattcaggta tcttgagcaa acgctccgtt gataatctaa
     7321 gtgtcggatt ccacgaagac catggagttg gtgcaccgaa gtcttatgtg attgagtatt
     7381 atgttggtaa gactgtccca acagctccta aaaaccctag ttttgttggt aatgaggacc
     7441 atgtctttaa tgattctgcc aactggaaac cagttactaa tctaaaagcc cctgctcaac
     7501 tcaaggctgg agaaatgaac cactttagct ttgataaagt tgaaacctat gctattcgta
     7561 ttcgcatggt taaagcagat aacaagcgtg gaacgtctat cacagaggta caaatctttg
     7621 cgaaacaagt tgcggcagcc aaacaaggac aaacaagaat ccaagttgac ggcaaagact
     7681 tagcaaactt caaccctgat ttgacagact actaccttga gtctgtagat ggaaaagttc
     7741 cggcagtcac agcaaatgtt agcaacaatg gtctcgctac cgtcgttcca agcgttcgtg
     7801 aaggtgagcc agttcgtgtc atcgcgaaag ctgaaaatgg cgacatctta ggagaatacc
     7861 gtctgcactt cactaaggat aagaacttac tttctcataa accagttgct gcggttaaac
     7921 aagctcgctt gctacaagta ggtcaagcac ttgaattgcc gactaaggtt ccagtttact
     7981 tcacaggtaa agacggctac gaaacaaaag acctgacagt tgaatgggaa gaagttccag
     8041 cggaaaatct gacaaaagca ggtcaattta ctgttcgagg ccgtgtcctt ggtagtaacc
     8101 ttgttgctga ggtcactgta cgagtgacag acaaacttgg tgagactctt tcagataacc
     8161 ctaactatga tgaaaacagt aaccaggcct ttgcttcagc aaccaatgat attgacaaaa
     8221 actctcatga ccgcgttgac tatctcaatg acggagatca ttcagaaaat cgtcgttgga
     8281 caaactggtc accaacacca tcttctaatc cagaagtatc agcgggtgtg atcttccgtg
     8341 aaaatggtaa gattgtagaa cggactgttg ctcaagccaa acttcacttc tttgcagata
     8401 gtggtacgga tgcaccatct aaactcgttt tagaacgcta tgtcggccca ggctttgaag
     8461 tacctaccta ctattcaaac taccaagcct acgaatctgg acatccattt aacaatccag
     8521 aaaattggga agctgtgcct tatcgtgcgg ataaagacat cgcagctggt gatgaaatca
     8581 acgtaacatt taaagctgtc aaagccaaag ccatgagatg gcgtatggag cgtaaagctg
     8641 acaagagcgg tgttgcgatg attgagatga ccttccttgc accaagtgaa ttgcctcaag
     8701 aaagcacgca atcaaagatt cttgtagatg gaaaagaact tgctgatttc gctgaaaatc
     8761 gtcaagacta tcaaattacc tataaaggtc aacggccaaa agtctcagtt gaagaaaaca
     8821 atcaagtagc ttcaactgtg gtagatagtg gagaagatag ccttccagta cttgttcgcc
     8881 tcgtttcaga aagtggaaaa caagtcaagg aataccgtat ccagttgact aaggaaaaac
     8941 cagtttctgc tgtacaagaa gatcttccaa aactcgaatt tgttgaaaaa gatttggcct
     9001 acaagacagt tgagaaaaaa gattcaacac tgtatctagg tgaaactcgt gtagaacaag
     9061 aaggaaaagt tggaaaagaa cgtatcttta cagtgattaa tcctgatgga agtaaggaag
     9121 aaaaactccg tgaagtggta gaagttccga cagaccgcat cgtcttggtt ggaaccaaac
     9181 cagtagctca agaagctaaa aaaccacaag tgtcagaaaa agcagataca aaaccaattg
     9241 attcaagtga agctgatcaa actaataaag cccagttacc aaatacaggt agtgcggcaa
     9301 gccaagcagc agtagcagca ggtttagctc ttctaggttt gagtgcagga ttagtagtta
     9361 ctaaaggtaa aaaagaagac tagaaatttt gataccttct ttatcattaa agaccaaaga
     9421 aaaactcttg tcctaaatag aagataaaga gtctggaaca aaatagttct caaccacaaa
     9481 aaagctagat atttccaatt gcggaactct agctttttaa ttttgagtcg ttctcttatt
     9541 gtatagtaga ttgaaactag aatagtacac ttctacttct aaaacattgt tagaaatcaa
     9601 tttgactgtc cttatcgatt tgtcctggtc ttatttcatt tcactatgtt ttaggaggtt
     9661 attggccata agtaccaatc ccatgtcaat tctcacttga cgcttccctc tcagattaca
     9721 tctcttgtaa cccaaacaag cctttatctg cccaaagaca ggtttcacat caatcttgcg
     9781 ttgagcgaaa tctgtctacc ttcaggagat aaaagcgcct gacattcttt agctttcaag
     9841 ttttgatagc gttcgttcat atacagtccc ttttgaggga ctgattcagg ttcatcagcg
     9901 tagtaaacct tgatttcctg ttgaaagtct gtctgtgttt tctgatgttt ggtatggtga
     9961 aaacgataat accagccatc aggatgtgtg tagctatcct ccttgtcatt atagtgccaa
    10021 ttcgctaagt ttttagctga ctgtttatgt cctctctttt gtttcttatc aaacatggca
    10081 tatttaatca gaaggggagg atttcttctt gaaaatagac ctgtatctgt tcttgaagtt
    10141 tggcggagaa tttctctgtc gttttcttcc acacaaaact atacttgttg gcattggctt
    10201 caatcttagt tccgtcaata aacaaacaat ctaaggtcac taactcttcc atttttcaat
    10261 ttttcgacca gagaaaatcc cttgtgaata ggcaaataga agagtagata caagcatttt
    10321 aaggtgataa gacgggcgat caaaggcatg atagaaggcg tagaagtgat gttcctccaa
    10381 ggtattcacc actttttcaa tagtaaagac gagatggtct tgtggtaaga aggaactgat
    10441 ttctagtggt aaagttgttt gatttgtgtt atagtgaata tgcatgggat agcctttcta
    10501 aatggtttat tgggcaattc tattttacca agactatcgt aaccatgcaa aaaaagcaac
    10561 ggcaaatccg ttgctttttt tggagataag agactattgt cccagactct tttctttgta
    10621 taaaattgta tagtgttatt gtctatacta ttttgtagag aaagcaaaaa tccctagaaa
    10681 atgcttaggg attcgactat ttcattaaga acaattcaac aatcatttct atatacatga
    10741 taagggttaa taagaatcct gcacgccaga agaatttgat gaatttagga tagtaaaagc
    10801 tgagtttttt caaaaggaag aaaaagacga gaataatggc taggattgag agggctagcc
    10861 ccagtctagg gaggaaatta tgggtaaagg ttttagctgt aatcaggtaa tattcaaata
    10921 ccaagaccgg aaaagccaaa tccgcaaagt ttcgtcctag tttttttaat ctaaaaagtt
    10981 tggttaggat aatgcagacg actaaggtca gtatcaataa taaaatagat gctaatttca
    11041 ttaaaatcat acccatattg tatcataaaa aaaagctaat ggaaacgaga aacagaggaa
    11101 tttataggaa agtcaggctt ttgagattgt gggtgatttt tgttataatg aaagttataa
    11161 aatcttataa taccttgaat gattattatc gaaaactctt tggagaaaag acttttaaag
    11221 tccctattga tgcggaattt gactgtccca atcgtgatgg aactgtggct catggaggct
    11281 gtactttttg tacggtttca ggttctggag atactattgt agccccagat gcgcctatcc
    11341 gtgagcaatt ttataaggaa attgacttta tgcaccgtaa gtggccggat gttcagaagt
    11401 atctggttta ttttcaaaat tttaccaaca cccatgaaaa ggtggaagtt attcgagagc
    11461 gttatgaaca ggctatcaat gagccaggtg tggtaggaat caatattgga atgcgcccag
    11521 actgtttacc agacgaaacc atcgaatatt tggctgagtt atcggagtgc atgcatgtga
    11581 cggttgaatt gggcttgtag actacttatg aagcaacttc tgacctgatt aaccgtgcgt
    11641 attcttatga attgtaggtg gaaacggtca agcgtttgag aaagtatccc aagattgaga
    11701 ttgtttccca tttgattaat ggtttgcctg gtgaaaccca tgagatgatg gttgaaaatg
    11761 tccgccgttg tgtcacgaat aacgatattc aagggattaa actgcatttg ctccatctta
    11821 tgacaaatac tcgtatgcaa cgagattacc atgagggacg tttacaattg atgagtcagg
    11881 acgaatatgt cagggtcatc tgtgaccaac tggaaatcat tcccaaacat atcgtcatcc
    11941 atcgaatcac aggagatgca cctagggata tgctgattgg gcttatgtgg agcctcaata
    12001 aatgggaagc tctcaacagc attgagatgg agatgcgacg tcgtggaagt gttcaaggat
    12061 gcaaggctgt aaaacaggag tttgaaaatg aaaagaccac ttgagatggc acatgatttt
    12121 ttggctgagg tcgtgacaaa agaggatgtc gtagtggatg cgactatggg aaatggtcat
    12181 gacacgcttt ttttagccaa gctagccaag caagtctatg cctttgatat tcagaagcaa
    12241 gccttggaaa agacccaaga gcgtttgcat caggctggct tgacaaatgc ccagttaatc
    12301 ttgcaaggcc atgagacact ggaccagttt gtgacaaaag ctaaggcagg gatttttaat
    12361 ttgggctatt tgccgtcagc tgataagtct gtcatcaccc gaccgcagac aacgattgag
    12421 gcattagaaa agctatgtgg cttacttgtc aagggtggac gaattgctat tatgatttac
    12481 tatggtcatg aaggaggcga cctcgagaga gatgctgtct tggattttgt aatccagttg
    12541 aaccaacaag agtacacagc tgccatttac cgaactttaa accaagtcaa caacccgccg
    12601 tttttagtga tgattgaaaa attagagaga tacagacatg gataaacaat acctacacga
    12661 aaagctggat gccatgcgcc agaattttgt tgaatcaacc caccacgaac gatcggtggg
    12721 cgtgctagac caagcgcata tgagcaaaaa aatgcttaaa atcaagaaaa aattagttgc
    12781 tcttgaaatg gaacggtgcc agagaaaaat tgagcacaaa gactgttcca agattgacca
    12841 aaaaatcaaa gagcagaagg agatatttga atcctgttgt aaaaaagatt aaggaggacg
    12901 tgcgtggaat tactgattta cctcatccta tttttactgg ttttgattgt ctcaagtaca
    12961 accaataagc tcctgccctt tttgcctctc cctttggtgc aaattctttt gggaattgtg
    13021 attggtctct ttttacccaa taccgacttt cacctcaata cggagttgtt tttggccctg
    13081 gttatcggac ccttgctttt ccgagaggct gaagaagcag atgttacggc tattttaaaa
    13141 cactggcgaa tcattgttta tctcatattt ccagtgattt ttatctcgac cctgagtttg
    13201 ggtggcttgg cccatcttct ttggttcagc cttcccttgg cagcttgctt ggctgttggg
    13261 gcagcccttg gtcctacgga cttggtggcc tttgcctctc tttcggagcg ttttagcttt
    13321 cctaagcgcg tgtccaatat tcttaagggc gaaggactct tgaatgatgc ttctggtttg
    13381 gtggcttttc aggtagcttt gacagcttgg acaactggag ctttttctct ggggcaagct
    13441 agcagttcgc tcatcttttc aatcctaggc ggttttttaa ttggattttt aacagccatg
    13501 accaaccgct tcctccatac cttcttgcta agtgtgcgcg caacggatat tgccagtgaa
    13561 cttttattag aattaagttt gcctctagtg accttctttc tggcagaaga agttcatgtt
    13621 tcagggatta ttgccgtcgt agttgctgga attttaaagg caagtcgttt caagaaaatc
    13681 acgctcctcg aagcccaagt ggatacggtg accgagacgg tctggcatac agtgaccttt
    13741 atgctcaacg gttctgtctt tgtgatttta gggatggagt tggaaatgat agcagaacct
    13801 atcttgacca atccaatcta taatcctcta cttttattgc tatctctcat cgcccttacc
    13861 tttgtcctct ttgtcattcg ttttattatg atctatggct attatgccta tagaacccga
    13921 cgcctcaaga aaaagctaaa taagtatatg aaggacatgt ttctcttgac cttttcaggt
    13981 gttaagggaa cggtgtcgat tgctacgatt ctcttgatac caagtaatct agaacaagag
    14041 tatcctcttt tgcttttcct tgttgcaggt gtgacgcttg tcagcttttt aacaggtctc
    14101 ttgatcttgc ctcatctttc tgatgaagag gaagaaagca aggattatct catgcatatc
    14161 gccattttga atgaagtaac gctagagttg gaaaaagagt tggaagacac cagaaataaa
    14221 cttcccctct atgcggctat tgacaattat catggacgta ttgaaaatct cattttaagc
    14281 caagaaaacc aggatgatca agaagactgg gctgctttga aactcttgat tcttagtatt
    14341 gaaagtgatg gtttggaaca ggcctatgaa gaggggaaca ttagcaatcg tgcttaccga
    14401 gtttaccaac gttatctgaa aaatatagaa caaggaatca atcgtaaact tgcctcaaga
    14461 ctgacctatt attttcttgt ttccttgagg attttacgtt ttctccttca tgaagttttt
    14521 actcttggaa agaccttccg tagctggaag gacaaggagc aaagccgtct ccgtgctctt
    14581 gattatgacc aaattgcaga gctctatctt gccaatacgg agatgattat tgaaagtttg
    14641 gaaaacctga agggagtcta cagacgctct ttgattagtt ttatgcagga gtctcgtctt
    14701 cgagaaacaa ctattatcag cagtggtgca tttgtcgaac gggttatcaa tcgtgtcaaa
    14761 cccaacaata tcaatgaaat gctgagaggc tattatctgg agcgcaagtt gattttcgaa
    14821 tacgaagaaa aacgattaat tacaactaag tatgccaaga aattacgaca aaatgtaaat
    14881 aacttagaga actattcctt gaaggaagct gccaataccc tgccgtatga tatggtggaa
    14941 ttggtaagaa gaaattagtt aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc
    15001 cttaccgtat gtatgtgact gacttcgtca gtcttatcta caacctcaaa acagtgtttt
    15061 gagcagcttg cggctagctt tctagtttgc tctttgattt tcattgagta taagattata
    15121 aatgaaggag tatgacatga aaaagtggtg gaaagagctg atagacaagc ctttattaaa
    15181 agcttttttg cattattatc aagcatcaga tagtgagttg accagtgtcg cagtagccta
    15241 ctattggttg atttcgattt tccccctgct tttggtggtg gtcaatatcc tcccctattt
    15301 tcagattcct gtgggagaat ttctgggctt tatgaaagac gtcctgcccc caagcctcta
    15361 tgaaggtgtg gaaaaaatag ccagagaagt cttgacgcaa ccttcaacag gtttattgag
    15421 tttttctgtt ttatcggcgt tatggagttt ttcaaaatct ataaatttct tgcaaaaagc
    15481 ttttaacaag gcttatggcg tcgaaaagag tcgtggactt atttcccatc agatgctaag
    15541 tctcctagtc agttttggct tgcagttcct ctttgcgttt gccttgtttt tgatcttatt
    15601 tggtcagatg attttggctt tacttgctca ttattggaca aaagatggca ttatctatca
    15661 ggctttacaa gggttggcag gtcctctgat ttacgccctc ctctttgcta tcttggtcat
    15721 gctttattat tttcttccca atctatctaa caggaaaatt agctataccc taccaggcag
    15781 tgcctttgtt ctcttggtga ttttaggtgt actaactctg ttctctagtt atctcaatta
    15841 ctatgtccat catttggtgg atgtccgaat tttaggttcc gttcttcttg ttgttatgat
    15901 gttttggttt atcttgattg ctaaaattgt tatcctaggc gcggtgatca atgccagtat
    15961 gcagagtttg aaagatcagg tctttaaaaa agactaacta tttatccatt acaaaagcgc
    16021 atagtatcaa ggctttatag aatcttgata ctatgcgctt ttgtgctttg acagtttttt
    16081 gtatagtttt tgtccaagat ttctaaatca tctgattaaa ttcctatttt cccctatcat
    16141 tgtccctgtt ttttcgaaaa tggaaggagt ataataaatc tatcaataaa atgataggag
    16201 gaactttatg ggtcatattt tcttttttct aagtgtcttt ttggcaggga ttctatcctt
    16261 cttttctcct tgtatcttac ctttgttacc ggtctataca ggagtgttac tagatgataa
    16321 ggatggtgct caggcttcta gcggcaaatt ttcaatctca gttactagtt tattacgaac
    16381 tctggccttt atagcaggaa tttcctttat atttattttg ttgggctatg gagctggttt
    16441 tttaggcgat ttgctttatg cttcttggtt ccaatatctt actggggcaa ttattatcct
    16501 tcttggtttg caccaaatgg agattctaca ctttaagggg ctttataagg aaaagaggct
    16561 acaactgcaa ggacaggggc aaaatggtaa gggctatagt caggcatttt tattgggctt
    16621 gacctttagt tttgcttgga cgccttgcgt ggggccggtt ctggggtctg ttttggcctt
    16681 ggcggcttca ggtggttcag gagcttggca gggagctggt ctcatgttgg tgtatacgct
    16741 gggcttggcg ctaccattct tgcttctagc tctgacctct agttatgttt tgaaacattt
    16801 ccgaaaactt catccctatc tcggaatcct caaaaaagtg ggtggttttc tcattattgt
    16861 gatgggattc ttggttctgt ttggaaatgc ttcaatttta agtcaattat ttgaataaaa
    16921 tggaaaggaa tatcaatatg aaaaaatggc aaacatgtgt tcttggagca ggttcgctcc
    16981 tttgtttgac ggcttgttca ggcaagtccg tgactagtga acaccaaacg aaagatgaaa
    17041 tgaagacgga gcagacagct agtaaaacaa gcgcagctaa agggaaagag gtggctgatt
    17101 ttgaattgat gggagtagat ggcaagacct accgtttatc tgattacaag ggcaagaaag
    17161 tctatctcaa attctgggct tcttggtgtt ccatctgtct ggctagtctt ccagatacgg
    17221 atgagattgc taaagaagct ggtgatgact atgtggtctt gacagtagtg tcaccaggac
    17281 ataagggaga gcaatctgaa gcggacttta agaattggta taagggattg gattataaaa
    17341 atctcccagt cctagttgac ccatcaggca aacttttgga aacttatggt gtccgttctt
    17401 acccaaccca agcctttata gacaaagaag gcaagctggt caaaacacat ccaggattca
    17461 tggaaaaaga tgcaattttg caaactttga aggaattagc ctaggaggcg tcttatgaat
    17521 gataagttaa aaatcttctt gttgctagga gtattttttc tagccataac cggtttctat
    17581 gttctattga tacgaaatgc agggcagaca gatgcctcgc aaattgaaaa ggcggcagtt
    17641 agccaaggag gaaaagcagt gaaaaaaaca gaaattagta aagacgcaga cttgcacgaa
    17701 atttatctag ctggaggttg tttctgggga gtggaggaat atttctcacg tgttcccggg
    17761 gtgacggatg ccgtttcagg ctatgcaaat ggtagaggag aaacaaccaa gtacgaattg
    17821 attaaccaaa caggtcatgc agaaaccgtc catgtcacct atgatgccaa gcaaatttct
    17881 ctcaaggaaa tcctgcttca ctatttccgc attatcaatc caaccagcaa aaataaacaa
    17941 ggaaatgatg tggggaccca gtaccgtact ggtgtttatt acacagatga caaggatttg
    18001 gaagtgatta accaagtctt tgatgaggtg gctaagaaat acgatcaacc tctagcagtt
    18061 gaaaaggaaa acttgaagaa ttttgtggtg gctgaggatt accatcaaga ctatctcaag
    18121 aaaaatccaa atggctactg ccatatcaat gttaatcagg cggcctatcc tgtcattgat
    18181 gccagcaaat atccaaaacc aagtgatgag gaattgaaaa agaccctgtc acctgaggag
    18241 tatgcagtta cccaggaaaa tcaaacagaa cgagctttct caaaccgtta ctgggataaa
    18301 tttgaatccg gtatctatgt ggatatagca actggggaac ctctcttttc atcaaaagac
    18361 aaatttgagt ctggttgtgg ctggcctagt tttacccaac ccatcagtcc agatgttgtc
    18421 acctacaagg aagataagtc ctacaatatg acgcgtatgg aagtgcggag ccgagtagga
    18481 gattctcacc ttgggcatgt ctttacggat ggtccacagg acaagggcgg cttacgttac
    18541 tgtatcaata gcctctctat ccgctttatt cccaaagacc aaatggaaga aaaaggctac
    18601 gcttatttac tagattatgt tgattaatac tcttcgaaaa tctcttcaaa ccacgtcagc
    18661 gtcgccttac cgtatgtagg gttactgact tcgtcagttt tatctacaac ctcaaagcta
    18721 tactttgagc tgacttcgtc agttttattt acaacctcaa agctatactt tgagaaacct
    18781 gtggctatct tcctagttta ctttttgatt tttattgagt ataagaggtc tttcctaagc
    18841 agttagagga gaattttgct atactgatac tagtaagtgg taaaggagca gagcatgacc
    18901 tacacaatct taatcgtaga agatgaatat ctggtaagac aaggtttgac taaactggtc
    18961 aatgtagcag cctacgatat ggaaatcatc ggtcaggctg aaaatggaag gcaggcttgg
    19021 gaattgatcc aaaagcaggt gccagatatc attttaaccg atatcaacat gcctcatcta
    19081 aatggcatcc agttggccag tctggtacga gaaacctatc ctcaggttca tttggtcttt
    19141 ttaacaggtt acgatgattt tgattatgcc ttgtctgctg tcaaactagg tgtggacgac
    19201 tacctgctca aacccttttc tcgtcaggat attgaggaaa tgttggggaa aatcaaacaa
    19261 aaactagaca aggaagagaa agaagagcag ttacaagatt tattgactaa taggtttgaa
    19321 ggaaacatgg cccagaaaat ccagtctcat ctggctgata gccaatttag tttaaagtct
    19381 ttagccagtg acttaggttt tagtccgacc tatctgagtt ccttgattaa gaaagagttg
    19441 ggcttgcctt ttcaggatta tctggtgaga gaacgtgtta aacaagccaa gctcttgctt
    19501 ttaactacag atctgaagat ttatgagatc gcagagaagg ttggttttga agatatgaac
    19561 tattttaccc aacgttttaa gcagattgca ggtgtgacac ctcgtcagtt taagaaggga
    19621 gaagaccgat gaagcgttct tctcttttag ttagaatggt tatttccatc tttctggtct
    19681 ttctcattct cctagctctg gttggaactt tctactatca atcaagttct tcagccattg
    19741 aggccaccat tgagggcaac agccaaacga ccatcagcca gactagccac tttattcagt
    19801 cttatatcaa aaaactagaa accacctcga ctggtttgac ccagcagacg gatgttctgg
    19861 cctatgctga gaatcccagt caagacaagg tcgagggaat ccgagatttg tttttgacca
    19921 tcttgaagtc agataaggac ttgaaaactg ttgtgctggt gaccaaatct ggtcaggtca
    19981 tttctacaga tgacagtgtg cagatgaaaa cttcctctga tatgatggct gaggattggt
    20041 accaaaaggc tattcatcag ggagctatgc ctgttttgac tccagctcgt aaatcagata
    20101 gtcagtgggt catttctgtc actcaagaac ttgttgatgc aaagggagcc aatcttggtg
    20161 tgcttcgttt ggatatttct tatgaaactc tggaagccta tctcaatcaa ctccagttgg
    20221 ggcagcaggg ctttgccttc attatcaatg aaaaccatga atttgtctac catcctcaac
    20281 acacagttta tagttcgtct agcaaaatgg aggctatgaa accctacatc gagacaggtc
    20341 agggttatac tcctggtcac aaatcctacg tcagtcaaga gaagattgca ggaactgatt
    20401 ggacggtact tggcgtgtca tcattggaaa agttagacca ggttcggagt cagctcttgt
    20461 ggaccttgct tggggccagt gtcacatctc ttcttgtctg tctctgctta gtgtggttca
    20521 gtcttaaacg ctggattgct cctttgaagg atttgagaga aaccatgttg gaaattgctt
    20581 ctggtgctca aaatcttcgt gccaaggaag ttggtgccta tgaactgaga gaagtaactc
    20641 gccaatttaa tgccatgttg gatcagattg atcagttgat ggtagctatt cgtagccagg
    20701 aagaaacgac ccgtcagtac caacttcaag ccctttcgag ccagattaat ccacatttcc
    20761 tctataacac tttggacacc atcatctgga tggctgaatt tcatgatagt cagcgagtgg
    20821 tgcaggtgac caagtccttg gcaacctatt tccgcttggc gctcaatcaa ggcaaggact
    20881 tgatttgtct ctctgacgaa atcaatcatg tccgccagta tctctttatc cagaaacaac
    20941 gctatggaga taagctggaa tacgaaatta atgaaaatgt tgcctttgat aatttagtct
    21001 tacccaagct ggtcctacaa ccccttgtag aaaatgctct ttaccatggc attaaggaaa
    21061 aggaaggtca gggccatatt aaactttctg tccagaaaca ggattcggga ttggtcatcc
    21121 gtattgagga tgatggcgtt ggcttccaag atgctggtga tagtagtcaa agtcaactca
    21181 aacgtggggg agttggtctt caaaatgtcg atcaacggct caaacttcat tttggagcca
    21241 attaccagat gaagattgat tctagacccc aaaaaggaac gaaagttgaa atatatataa
    21301 atagaataga aactagctaa ctcccagtca attctgggag ttttatgcgt aattgggcaa
    21361 actttctagg ttgtctatct tgctaaaaac aagaaaaaac ggtatgatag aagagtgaca
    21421 acagaggtaa cagagatgaa cgagacacag attcaaagag aaacacgtca ggtagtggaa
    21481 gatgttttag aaaagaccaa tttgaaacag ggggcccttt ttgttttagg tctatcttct
    21541 agtgaggtgc taggtggtca gattgggaaa gaatccagtc aagagattgg tgagctgata
    21601 gtggagacta tcttgggtat tctaggtagt agaggtatcc atttagcagt tcaaggttgt
    21661 gagcatgtca atcgggctct cgttgttgaa cgtcaggtgg cagagcagtt tggtctggaa
    21721 attgtcagtg tccatcctac tcttcatgca ggaggttcgg ggcagttggc agcctttaag
    21781 tttatgcagg atccagttga ggttgaattt atcaaggctc atgctggatt ggatatcgga
    21841 gacactgcaa ttggcatgca tgtcaagcat gttcaggttc cgattcgccc tattttgaga
    21901 gagattggtc atgcccatgt aacggcactg actagtcgtc caaaattaat cggaggtgcg
    21961 cgtgcgcact atccgcaaga tgctattagg aagttttgag aatgaataaa ggagataaaa
    22021 gtgtttaaaa aagaccgttt ttcaattcgt aagattaagg gagttgtagg ctctgtattt
    22081 cttggaagcc ttttgatggc tccttctgta gtggacgcag ccacctatca ctatgtaaat
    22141 aaagagatta tttcacaaga agctaaagat ttaattcaga caggaaagcc tgacaggaat
    22201 gaagttgtat atggtttggt gtatcaaaaa gatcagttgc ctcaaacagg gacagaagca
    22261 tctgttttga cagcttttgg tttgctgact gttgggagct tgcttttaat ctacaagaga
    22321 aagaaaattg ctagcgtctt tctagttgga actatgggat tggtagttct tcctagtgca
    22381 ggggctgtag acccagttgc gaccctagca ctggctagtc gagagggtgt tgttgaaatg
    22441 gagggctatc gctatgttgg ttatctatca ggtgacatcc tcaaaacgct tggcttggac
    22501 actgttttag aagaaacctc agcaaaacct ggagaggtga ctgtagtcga agttgagact
    22561 cctcaatcaa caacaaatca ggagcaagct aggacagaaa accaagtagt agagacagag
    22621 gaagctccaa aagaagaagc acctaaaaca gaagaaagtc caaaggaaga accaaaatcg
    22681 gaggtaaaac ctactgacga cacccttcct aaagtagaag aggggaaaga agattcagca
    22741 gaaccagctc cagttgaaga agtaggtgga gaagttgagt caaaaccaga ggaaaaagta
    22801 gcagttaagc cagaaagtca accatcagac aaaccagctg aggaatcaaa agttgaacaa
    22861 gcaggtgaac cagtcgcgcc aagaaaagac gaacaggcac cagtcgagcc agaaaatcaa
    22921 ccagaagctc ctgaagaaga gaaggctgta gaggaaacac cgaaacaaga agagtcaact
    22981 ccagatacca aggctgaaga aactgtagaa ccaaaagagg agactgttaa tcaatctatt
    23041 gaacaaccaa aagttgaaac gcctgctgta gaaaaacaaa cagaaccaac agaggaacca
    23101 aaagttgaac aagcaggtga accagtcgcg ccaagagaag acgaacaggc accaacggca
    23161 ccagttgagc cagaaaagca accagaagtt cctgaagaag agaaggctgt agaggaaaca
    23221 ccgaaaccag aagataaaat aaagggtatt ggtactaaag aaccagttga taaaagtgag
    23281 ttaaataatc aaattgataa agctagttca gtttctccta ctgattattc tacagcaagt
    23341 tacaatgctc ttggacctgt tttagaaact gcaaaaggtg tctatgcttc agagcctgta
    23401 aaacagcctg aggtaaatag cgagacaaat aaacttaaaa cggctattga cgctctaaac
    23461 gttgataaaa ctgaattaaa caatacgatt gcagatgcaa aaacaaaggt aaaagaacat
    23521 tacagtgata gaagttggca aaacctccaa actgaagtta caaaggctga aaaagttgca
    23581 gctaatacag atgctaaaca aagtgaagtt aacgaagctg ttgaaaaatt aactgcaact
    23641 attgaaaaat tggttgaatt atctgaaaag ccaatattaa cattgactag taccgataag
    23701 aaaatattgg aacgtgaagc tgttgctaag tatactctag aaaatcaaaa caaaacaaaa
    23761 atcaaatcaa tcacagctga attgaaaaaa ggagaagaag ttattaatac tgtagttctt
    23821 acagatgaca aggtaacaac agaaactata agcgctgcat ttaagaacct agagtactac
    23881 aaagaataca ccctatctac aactatgatt tacgacagag gtaacggtga agaaactgaa
    23941 actctagaaa atcaaaatat tcaattagat cttaaaaaag ttgagcttaa aaatattaaa
    24001 cgtacagatt taatcaaata cgaaaatgga aaagaaacta atgaatcact gataacaact
    24061 attcctgatg ataagagcaa ttattattta aaaataactt caaataatca gaaaactaca
    24121 ttactagctg ttaaaaatat agaagaaact acggttaacg gaacacatgt atataaagtt
    24181 acagcaatcg cagacaattt agtctctaga actgctgata ataaatttga agaagaatac
    24241 gttcactata ttgaaaaacc taaagtccac gaagataatg tatattataa tttcaaagaa
    24301 ttagtggaag ctattcaaaa cgatccttca aaagaatatc gtctgggaca atcaatgagc
    24361 gctagaaatg ttgttcctaa tggaaaatca tatatcacta aagaattcac aggaaaactt
    24421 ttaagttctg aaggaaaaca atttgctatt actgaattgg aacatccatt atttgacaag
    24481 attaaagctg gaactgttaa aaatataaat tttgaaaata ttgatattaa tgtgtctaat
    24541 caagataata tcgcgacaat tgcgaatgaa atcaacggaa ccacacgtat agaaaacatc
    24601 aaagcaactg gaacgataac agccagaaat aacgttgctg gatttgtaaa caatatcaaa
    24661 gaaaatgctg ctctagaaaa cgtagcattt ataggtaata tcaattctgt tggtaataat
    24721 tctacagtcg gaggaattgc tggttcgaac tacatgggat ttgttaataa agcttatgtt
    24781 gatgctaaca ttactgccgc taacgcaagc gcaagtatgc tagttccttt ggttacatat
    24841 atgttaaata gctatcagta tggaactaag tcaaaagtta ccaattcggt tgctaaagga
    24901 actttagatg ttaaaaatac tcgttttgtt ggtggtgttg tagctaaaac ttggccttgg
    24961 ggagttataa aagatattgt tacctatgct aaagtaatta aaggtgaaga agtatatggt
    25021 tcaaacgaaa ttaatgacgg agatgcacgc caattcttat ctgatattta tagagtcaac
    25081 ggcgctagca gtggagctaa taattttacc aatctgcaaa aaataaaaca attaactaaa
    25141 gaacaagctg atgctaaagt tgctactttc aatattactg ctgataaatt agaaagtgag
    25201 ctatctcctc ttgcaaaact taatgaagaa aaagcttatt ctagtattca agattacaac
    25261 gctgaatata atcaagccta taaaaacctt gaaaaattaa taccattcta caataaagat
    25321 tatattgtat accaaggtaa taaattaaat aaagaacacc ctctaaatac taaagaagtc
    25381 ctttctgtta cagcgatgaa caacaatgag tttatcacaa acctagatga agctaataaa
    25441 attattgttc actatgcaga cggtacaaaa gattacttta acttatcttc tagcagtgaa
    25501 ggtttaagta atgtaaaaga atatactata actgacctag gaattaaata tacacccaat
    25561 atcgtgcaaa aagataacac tactcttgtt aatgacataa aatctatttt agaaccagta
    25621 gatcttcaat ctcaaacaat gtatcaacac ctaaatcgat taggtgacta tagagttaat
    25681 gcaatcaaag atttatattt agaagaaagc ttcacagccg ttaaaaagaa cttaacaaac
    25741 ctaatcacaa aattagttca aaatgaagaa caccagttaa atgattctcc agctgctcgt
    25801 caaatgcttc gtgataaagt cgagaaaaac aaagctgccc tattattagg tttaacatac
    25861 ttaaaccgtt actacggtgt taaattcggt gatgttaata ttaaagaatt aatgttattc
    25921 aaaccagact tctatggtga aaaagttagc gtattagaca gattaattga aatcggctct
    25981 aaagagaaca acattaaagg ttcacgtaca ttcgacgcat tcggtgaagt attagctaaa
    26041 tatactaaat caggtaattt agatgtattc ttaaattata atagacaatt attcacaaat
    26101 atagataata tgaatgattg gtttattgat gctacaaaag accatgtcta catcgcagaa
    26161 cgcgcatctg aggtcgaaga aattaaaaat gctaaatata gagcatatgc taacttaaac
    26221 gtagctcagc acagaaaaat gattttacca ttattgaata taactaaatc acacatgttt
    26281 attgcctcta actacaacgc aattgcaatt ggtagtgctg agaaattagg aaaaaaatca
    26341 ttagaagaca ttaaagcatc tgttgattat gctgctgatg ggtacagaaa ctactatgat
    26401 ttttggtatc gtctagttcc agataatgtt aaacaatatc tactaagaga tactgtgatt
    26461 cctatctggg aaggttataa tgcgccaggt ggctgggttg aaaaatacgg tcgatataat
    26521 actgacaatg tatatactcc tcttagagaa ttctttggac ctatagaaaa atattatggt
    26581 tataatggaa caggagcata tgctgcactt tatgataatc caatggatcc tcgttctgat
    26641 gtaagttatg ttgctctaaa tatgattggg gattatggta tttctgtgta tactcatgaa
    26701 acaacacata taaacgatcg taaaatctat ttgggtggtt ttggacgtcg tgaaggtact
    26761 gatgctgaag catttgctca aggtatgtta caactaccag tcccaggaag tggatttaac
    26821 gaatatggtt cattaggtct gaataccgta ttcaaaaaac ctaatgatgg taatcaatgg
    26881 tacgatacag atcctaaatc tcttacaaca cgtgatgata tcgataaata tatgcgtggt
    26941 tacaatgatg ctttaatgct tgtcgatcat ttagaggcag atgctgttat taatcaaaat
    27001 aataaagagt taaatggagc atggttcaag aaaatagata agaaattccg agatgccagc
    27061 caattaaatc agtgggacga aattcgaaat ctaacaactg aagaaaaaga taacttaaat
    27121 attacatcaa ttgatagttt aatagatcaa cagttaatga ctaatagaca aattggaaac
    27181 cgtgtttata aacctgaagt cgttagccaa tacgatcaat caccttatgt gggggttgaa
    27241 atgacagccg gtatctacgg aggtaatact agtaaaggtg ctcctggagc tgtttcattc
    27301 aaacataacg cctttagatt atggggttac tacggatacc ttaacggatt cctaggctat
    27361 acttcgaata aatataaaca acaatctaca aaagatggcg agtcagttct aagtgatgaa
    27421 tatattatca agaaaatatc taataataca ttcaacacta ttgaagaatt taaaaaagct
    27481 tatttcaaag aagttaaaga taaagcaacg aaaggattaa caacattcga agtaaatggt
    27541 tcttccgttt catcatacga tgatttactt acactattta aagaagctgt taaaaaagat
    27601 gctgaaactc ttaaacaaga agcaaacggt cacaaaacag tatctatgac taatacagta
    27661 aaactaaaag aagctgttta taagaaactt cttcaacaaa cagatagctt taaaacttct
    27721 atctttagat aatcgaaatc tctcatctgc tttgcggatg agggttttct tattttatgc
    27781 tatacttaag gtatgcatag aaaaacagtg attgatttta ggactttagg ggagagatat
    27841 acctttaccc agcctattaa agagttgaaa acgagaaatg tagaagaagt ggcagatttg
    27901 ctggcacaag tggaaagcta ccaagagcaa ggttattatg tggtggggta tgtcagctac
    27961 gaggctgcac ctgcttttga ggaaaaatta gcagttcaca aggatcccct actgggagag
    28021 tacctgcttt attttactgt tcatgatagc gtagaaactt cccctattcc tctgacttat
    28081 gaggatattg atttgccctc aaattggcag gaagtaacgt ctgcagcaga ctatgaaaag
    28141 gccattgccc agatacacca tcacttgagg cagggagaca cctatcaggt caactacacc
    28201 gtccaactca agcaaaagtt aagtgccaat ccttttgcca tctacaatcg tatggtggta
    28261 gagcaggagg cgggctacaa tgcctatgtg gaacatgacg agatggcagt gatttccatg
    28321 agcccagagc tcttttttga gcaaaatgat cgcgagttga caacacgacc aatgaagggg
    28381 acgactcagc gtggggtaac tgaccaagaa gatcttgaac aggccagttg gttggaacag
    28441 gatcccaaaa atcgctctga aaatatgatg attgtggacc tcttgcgcaa tgatatgaac
    28501 cgtatttctg aagttgggag cgagcacgtg gagcgtctgt gccaggtaga gcagtattca
    28561 actgtttggc agatgacttc gaccatcaag agtcagttgc gagaggatgt ggaccttgtt
    28621 gaaatcttcc gctcactctt tccttgtggt tccataacgg gtgcaccgaa aattgcgaca
    28681 atggagatta tcaagaactt ggagcctcaa ccgcgtggag tctactgtgg aacgattggt
    28741 ctcttgcttc caaatggacg acggattttt aatgtggcca ttcgtaccat tcaacttcac
    28801 aaaggtcaag ccatttatgg agttggcgga gggattactt gggatagcac atgggaatct
    28861 gaataccgag aggttcatca aaaggcagca gttctttatc gtaaacaagc tcgcttccaa
    28921 ctgattacga caggggaaat cagccagaag aacctgctgt ttgaagatca acatctggaa
    28981 agactgagaa aagctagtcg ttattttgcc tttccttttg atgcagaaga cttgggacac
    29041 aagattgagg aagagtgtca ggattgtgaa gctaatcaag attaccgctt gcgaatcagc
    29101 cttagcaaat ctggagagat agaagtcaat cgtcaagtat taacccctct cagtacaagc
    29161 ttttgtcagg cccaagtctg ccttcaggaa gctgctttga atcaatcctt tacctacttt
    29221 aaaaccactc accgaccgca tttgagccta ggagaacaag agaagattta ccacaataag
    29281 tcaggagaac tgcttgaaac ctctatagga aatttggttc tgaaaatcgc tggaaaactc
    29341 tacacaccgc ctatccgact tggaatcttg ccaggaattt accgtcagca tttgctagaa
    29401 acaggacagg tagaagagaa agtcttgacc ttggcagatt taacccaagc agaagctatt
    29461 tacggctgta atgcagtgag aggcttgtat gaactaagcc ttgaggagaa ctagatgaaa
    29521 ttgatatttt tgcatgggtt agggcagtca gcagagtctt ggaaagaagt tcggaatcta
    29581 ctgacagatt atccttctga ggctatcgag ttatttcctt ctggagttag taactatcaa
    29641 caagctaaag agcgagttta tcagcaccta gctcaagaga cagaaccttt tgttttgatt
    29701 ggattgtcct taggtgctgc cctgacacta gaactttcca gttacgattt accaaatctt
    29761 cgagctttga ttctgtcagg atgcccgctt aaattagcag ggaatatcct attttatctt
    29821 cagttgctga tttttaaact acttcccaaa agagtatttg aaaaacaggg agcagacaag
    29881 actcttatgg tcggagtttc tgaggaattg aagacacttg atttaacgga tatagcaggg
    29941 acttgctcct atccaacctt actaatttgt ggcagcaaag ataaaccgaa tcttagttct
    30001 atgaaagctc tccatagact gttaacagat tcgcagtttc agatcattcc tgatggccct
    30061 catgtcttga ataaggcaaa accaaaggag tttgtagaaa agaccagaag tttccttgaa
    30121 ttgctgaaat aagtttgata aaataatatt gaaatcgata tcataattat gggagaaaga
    30181 aatgaataaa cgtctatttt taaaaatgag tctggtgacg ttgccaattt tagccttgtt
    30241 ttcacaacca gttttggcgg aagaaaacat ccatttttcg agctgtaagg aagcttgggc
    30301 gaatggctat tcggatattc acgagggaga acctggttat tctgccaagt tagaccgtga
    30361 tcatgatggt gtggcttgcg aattgaaaaa tgctcctaag ggtgctttta aagcaaaaca
    30421 gtcaacggct attcaaatca acacaagttc agcaacaaca agtggttggg ttaagcagga
    30481 cggcgcttgg tactactttg atggaaatgg aaatctagtg aaaaatgcat ggcagggaag
    30541 ctattacctg aaagctgatg gtaaaatggc acagagtgaa tggatttatg actcttctta
    30601 tcaagcttgg tattatttga catcagatgg ttcttatgca aaaaatgcat ggcaaggagc
    30661 ttattacctt aaatcaaacg gtaaaatggc acaaggtgag tgggtttatg attcttctta
    30721 ccaagcatgg tattacttga aatcagatgg ttcatatgct cgcaatgcat ggcaaggaaa
    30781 ctactatttg aaatcagatg gtaaaatggc taaaggtgaa tgggtttatg atgccaccta
    30841 tcaagcttgg tattatttga catcagatgg ttcttatgct tacagtacat ggcaaggaaa
    30901 ttactatcta aaatcggatg gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta
    30961 ttatgttggc gctgacggag tttggaagga aggtcaagca agtacagttt cttctagtaa
    31021 tgatagcaat agtgaatatt ctgctgcttt aggaaaggca aaaagttata attcgttatt
    31081 ccacatgtca aaaaaacgta tgtatagaca attaacttct gattttgata aattttcaaa
    31141 tgatgcagct caatatgcca ttgatcattt agatgattaa cagatactgg taggcgaaaa
    31201 aattccttat aattcattta cgcaaacctt tgcataagtg aatggatttt gatatactag
    31261 tactgtaagc attttcaatt actacacaat gaaaaaggag aatatgatga gtcaaaagat
    31321 tattgggatt gaccttggtg gaacttctat caaatttgca atcttaacaa cagtaggaga
    31381 aatccaagga aaatggtcaa tcaagaccaa cattttggat gagggaagtc atatcgttga
    31441 tgatatgatt gagtctattc aacatcgttt ggacttgctt ggattggcag cagcggactt
    31501 ccaaggcatt ggaatgggat caccaggtgt ggttgaccgt gacaaaggga ctgttatcgg
    31561 tgcctacaac ttgaactgga aaacccttca accaatcaaa caaaagattg aaaaagcttt
    31621 gggcattcca tttttcatcg ataatgatgc caacgtagca gctcttggtg agcgctggat
    31681 gggtgctgga gataaccaac cagacgttgt ctttatgaca ctcggtactg gtgttggtgg
    31741 tggtattgtc gcagaaggca aattgcttca tggtgttgct ggtgcagcag gtgaacttgg
    31801 tcatatcact gttgactttg accaaccaat ctcatgtact tgtggtaaaa aaggctgcct
    31861 tgagacagtt gcttcagcaa cagggattgt caacttgact cgtcgctatg ccgatgaata
    31921 cgaaggcgat gcagccttga aacgcttgat tgataacgga gaagaagtaa ctgctaagac
    31981 tgtctttgat ctcgcaaaag aaggagacga ccttgctttg attgtttacc gtaacttctc
    32041 acgttacttg ggaatcgctt gtgctaacat tggttcaatc ctaaacccat caacaatcgt
    32101 catcggtggt ggtgtgtcag ctgcgggaga attccttcta caaggtgttc aaaaagttta
    32161 cgatgaaaat agtttcccac aagtacgcac atctactaaa ttagctcttg caactctagg
    32221 aaatgacgct ggagttatcg gagcagcatc acttgtattg caataagata atgagagagg
    32281 aaaatgctaa cctagagtta gtaatgttcc tcttttattt tttatgctat actagttagg
    32341 acaatgaatg aggtgaaacg aaatgacaaa agcagatacg atttttaaag agaatattga
    32401 acgcattcta aaggaaggtg ttttctcaga gcaggctcgt cctaagtaca aggatgggac
    32461 tgttgctaat tccaagtatg taactggtgc cttttccgag tacgacttat ctaaaggaga
    32521 atttcctatc acaaccttgc gtcccattgc aatcaaatcc gccatcaaag aagttctctg
    32581 gatttaccaa gaccagtcaa atagcttaga agtgcttaat gacaagtaca atgttcacta
    32641 ctggaatgac tgggaagttg gagacacggg aaccattggt gagcgctatg gtgccgttgt
    32701 taagaaacac gacattatca ataagcttct caaacagttg gaaaccaatc cttggaaccg
    32761 ccgcaatatt atttcgctct gggattacca agctttcgaa gaaacagatg ggctgctccc
    32821 gtgcgccttt cagaccatgt ttgatgtccg ccgtatagat ggagaaatct atctggatgc
    32881 gaccttgacc cagcgctcga atgatatgct ggtggcccac cacatcaacg ctatgcagta
    32941 tgtggctttg cagatgatga ttgccaaaca ttttggctgg aaggttggga agttcttcta
    33001 cttcatcaac aacctccata tctatgataa tcaatttgaa caagctcagg aattgctccg
    33061 tcgggagccg tcaaactgcc aaccacgctt ggttttaaat gttcctgatg ggactaattt
    33121 ctttgatatc aaagcagaag attttgagtt ggtggattat gaccctgtta agccacagtt
    33181 gaagtttgac ctagctattt aaaagaatag aaaaaagaag ttgagaataa tcccaacttc
    33241 ttttgtttct taacgtgata ctcggcgacg agctgctttt ttacggtttt cttcgatgaa
    33301 agctgctttt tgctcttctg gttcgattac tttcttttta aatgcgtata ctgcacctgc
    33361 aacggcagcg acagttcctg cgacacctgt tacaagacct ttagcgaatc ctttagccat
    33421 gagtcttcct cctttatatt ctcaatcagc cagcctcctc aagaggtcac atttttctga
    33481 ctgacctttt tgtgttataa taatagtaac gaaaaaatgg gaatttttca aggaaaaaag
    33541 atgaaaacaa aaataattgt gattgttgga ccgactgctg ttggaaagac ggctctagct
    33601 attgaagttg caaagcgttt taatggcgaa gtggttagtg gagatagcca acaagtctat
    33661 cgaggacttg atattgggac agctaaggct agtccagaag agcaggcagc tgttcttcat
    33721 catttaatcg atgttagaga gataaccgag tcttactcgg cttttgattt tgtttcagaa
    33781 gctaagatga ctattgagga tattcacagc cgtggcaagc tagccattat cgccggtggg
    33841 actggacttt atatccagag cttgctagaa ggttaccacc taggtgggga gactcctcat
    33901 gaggagattt tagcttatcg agctagtttg gagccgtatt cagatgagga attagcccat
    33961 tttgtggagc aagcaggcct tgagattccc cagtttaatc gtcgtcgtgc tatgcgtgcc
    34021 ttagaaattg cccattttgg tcaggatttg gaaaatcaag agaccttgta tgaaccgctg
    34081 attatctgct tggatgatga acgtagtcaa ctttatgagc gtatcaacca ccgagtggat
    34141 ttgatgtttg aggctgggct tttggatgag gccaagtggc tgtttgacca ttcccctaat
    34201 gtacaggctg ctaaaggaat tggctataag gaactctttc cttatttccg tggagagcag
    34261 acctttgagg aagctagtga gagtcttaaa caggcgaccc gtcgttttgc caagcgtcag
    34321 ctgacctggt tccgtaatcg catgcaggtc accttttatc agattggaga atctggtgta
    34381 caagaccgca ttttaagcca gatagaggag tttttagatg attgaaacgg agaaaaaaga
    34441 ggagcgagtc ctgctgattg gtgtggaatt gcagggtatg gacagttttg acctctccat
    34501 ggaagaattg gctagtttag cgaaaacggc aggggcagtc gttgtagata gctacagaca
    34561 aaaacgtgaa aaatatgatt ccaagacctt cgtcggctct ggtaagttgg aagagattgc
    34621 gcttatggtg gatgcagaag aaatcactac tgtcatcgtc aacaaccgtc tgaccccaag
    34681 gcagaatgtc aatctagagg aagttctcgg tgttaaggtc attgaccgta tgcagttgat
    34741 tttggatatc tttgccatgc gggctcgaag ccatgaaggg aagttccaag tccacctagc
    34801 ccaactcaaa taccttttgc ctcgcttggt tggtcagggg attatgctca gccgtcaggc
    34861 agggggaatt ggttcccgtg gtcctggtga aagccagctg gagctgaacc gtcgtagcgt
    34921 tcgcaatcaa atcacggata tcgagcgcca gctcaaggtg gttgagaaaa atcgtgcgac
    34981 tgtcagagaa aaacgtttgg agtctagcac ttttaagatt ggtttgattg gttataccaa
    35041 tgctgggaaa tcaactatca tgaacatctt gaccagtaag acccagtatg aagcagatga
    35101 gctctttgcg actctggatg cgacaaccaa gagtattcat ctgggaggca atctccaagt
    35161 aactttgaca gataccgttg gctttatcca agatttgccg acagagttgg tgtccagttt
    35221 caagtcaacc ttggaagaaa gcaagcatgt ggaccttctg gttcatgtta tcgatgctag
    35281 caatccttac cacgaggagc atgaaaaaac ggttctctcc atcatgaaag acctggacat
    35341 ggaagatatt cctcacttga cgctttataa taaagcggat ttggtggagg atttcacgcc
    35401 tacccaaacg ccatataccc tcatttctgc caagtctgag gacagtcgtg aaaacttgca
    35461 agcattattg ctagataaga ttaaggaaat ttttgaagca tttaccctgc gagtgccttt
    35521 ttcaaagtcc tacaagattc atgatttaga gagtgttgca attctggaag aacgtgatta
    35581 tcaggaagac agcgaagtga ttacaggcta catttccgaa aaaaataaat ggaggttaga
    35641 agaattttat gactgatatt aaagctttgg ctctaaagta tggaggttat acaagtctgg
    35701 acaaggtcta tctggttcag cttctagctg gtaaaacaga gcaggagcag ttaacactta
    35761 ttacacctcc gccaagtgtt gttaatgcct actttgcaga actctatcag aaaaagagtc
    35821 ctcaggctgc gacggattat tttgcagaac tcagtcagga actgaatctt tacaatactg
    35881 agccaagttt caccctagaa aataagcctt ttattcggct taatctgtct ggtaaatcct
    35941 ttggtttttg ttatgatagt gacggccttg gtcggatttt ctctgaaaat gaggagaaaa
    36001 tctcggatga cttgctcttt gaaattgcgc aaattttccc ccatcaatta gtctttgaag
    36061 agtctggtaa gatttacatg aagcctgtcg aggacgagga agttgtcagc ctagaaagtc
    36121 tcacagcttt gactgatttg gaaagattgg ctgatggtcg aaaacgtctc aaaggctaca
    36181 gccaagagga tttattgcaa gaagctgctg ctttttctgg caaacgctat ttccgatcgg
    36241 aaaaccgcac agccatgtta tatattgatt aattagaaag tatcgaatgg atattcaatt
    36301 tttaggaacg ggggctggtc agccctctaa agctcgcaac gtttcaagtc tcgccctgaa
    36361 acttttggat gagattaacg aagtttggct ctttgactgt ggagaaggta cgcaaaatcg
    36421 cattctggaa accacagttc gaccacgtaa gatcagcaaa atctttatta cccatctgca
    36481 tggagaccac atttttggtt tgccaggttt cctttctagc cgtgcctttc aggccaatga
    36541 agagcagaca gatttggaaa tctacggacc tcaaggaatc aagtcatttg tcttaaccag
    36601 ccttcgtgtg tcaggttctc gtctgcccta ccgcattcat ttccatgagt ttgaccaaga
    36661 ttctctagga aaaatccttg agaccgataa attcactgtg tatgcagagg agctggacca
    36721 tactattttc tgtgttggct atcgtgtcat gcaaaaggat ctagaaggga cgctggatgc
    36781 tgaaaaactc aaggctgctg gtgttccgtt cggcccgctt tttggtaaaa tcaaaaacgg
    36841 ccaggatctt gttttggaag acggaactga aatcaaggcg gctgactata tctcagcgcc
    36901 acgtccaggt aagattatca ctattttagg agacactaga aaaaccaatg ctagtgtgcg
    36961 tctggctgtc aatgcagatg tcctagttca tgagtccact tatggcaagg gtgatgaaaa
    37021 aattgctcgt aaccatggtc actcaactaa tatgcaagct gcacaagtag cggtagaagc
    37081 aggtgccaaa cgcctcctac tcaaccatat cagtgcccgt ttcctctcaa aagatattag
    37141 caaactcaag aaagacgctg ccacaatttt tgaaaatgtc catgtggtca aagacttgga
    37201 agaagtggaa atctagcagt cacagaaagg ataagtatgc ctactattct cattaccgga
    37261 gctagcggtg gtctagctca agaaatggtc aaactcctgc ccaatgacca actcatcttg
    37321 cttggtagaa ataaggaaaa attagcccaa ctctacggaa attattccca tgcagaattg
    37381 attgaaattg atattaccga cgattcagcc ctagaagctc tggtaactga tctttatctc
    37441 cgctatggca agattgatgt cttgattaac aacgctggtt acgggatttt tgagggattt
    37501 gaccagattg ctgataaaga cattcaccag atgtttgagg tcaatacctt tgccctgatg
    37561 aatctgtctc gtcaccttgc ggctcgtatg aaggaaagca gcaaagggca tatcatcaac
    37621 atcgtcagca tggcaggtct aatagctact ggcaagtcta gtctttactc agcgaccaag
    37681 tttgcggcta ttggtttttc aaatgctctg cgactcgaac ttatgcccta tggagtctat
    37741 gtgacaacag tcaatccagg tccaatccga acaggatttt ttgaccaagc tgacccagat
    37801 ggaacttatc ttaaatcggt tgaccgcttc ctcttagagg cagatgcagt ggctaaaaag
    37861 attgtcaaga ttataggaaa aaataaacga gaactcaatc tcccgatttt gttgaaccta
    37921 gcccataagt tttatactct ctttcccaag ctagctgata agttggcagg ggaaactttt
    37981 aattataagt aaaaagaacc aatgtgcagg ttgttgctag cctacatatt ggttcttatc
    38041 atttctcaac tatcaaactg aacttcttct agtagatagt cgataaagcg ttcgcccatc
    38101 ttagataggc tggttttctc atgctggata tagaccagct caatcgggtc atcaatatcc
    38161 agtgggatag aaacgatatt gtctccgttt aggttgctgt tcaaaatccc tgtcgcaatg
    38221 gtataaccat ccaaaccaat caagagatta aagagggtgg cacggtcact gaccacaatg
    38281 gatttcttgt ggtgttcttg agaaagaatc tcttctgaaa agtagaagga gttgtgcgtc
    38341 ccttggtcat agctgaggta agggaaattc tccaaatcag acagtttcac cttgtctttc
    38401 tttgccagag ggttggtctt gctgacaaag atatgcggtt gcgctgtgaa gagatggtgg
    38461 gatagcaggt gattgtcatc cagcatcttg gttaaaacat cacggttgta actgtttaag
    38521 aagaggaccc cgacctcact gcggaagttc ttgacgtcgt cgataatctc ccaagtccga
    38581 gtttcacgaa ggaagagttc gtatttctcc atatcgcttt tcttgagcaa agagacaaag
    38641 gcattgacca caaaggcata gtgttgagac gaaacgctaa agagttcgcg gtgggcgaca
    38701 ggatttttat agcgttcctc cagaagctga gtttgctcga caacctgacg ggcataagag
    38761 agaaactcca tcccatcacg ggtcaaggta atacccttgg gattgcggat aaagatttca
    38821 atgcccattt cattttccaa atctcgcact gcattggaga gactgggctg agtgataaag
    38881 agttgcttgg ctgcctcatt catagagcca gtttcgacga ttttgataat ataatgtaat
    38941 tgttgaattc tcatgttttt attgtaccac acttgcctta gaatcgccaa agaagatagg
    39001 aaaataagga gcattgtgtg agtctcttct tgaaaaaaac acaaaatttt gctagaatga
    39061 gtcttatgaa aacattctat gatgtgcagc aattcctcaa acgatttggt attattgttt
    39121 acatgggaaa acgcttatat gatattgaac tgatgaagtt ggaactatct cggatttacg
    39181 atgcagggtt gatggataaa ttagactatc tagaggcaga agcagttctt cgcagagagc
    39241 acaaggtaga attggattat attgagaaaa atggagaaaa gaactaatgg ttacttggat
    39301 tttgtgggca cttatactag caatgttggc gtggatgggc tttaactatc ttcgtattcg
    39361 ccgtgcggct aaaattgtgg acaatgagga gtttgaagcc ttgattcgta cgggtcaatt
    39421 gattgatttg cgcgacccag cagaattcca cagaaaacat atccttcgtg cacgcaatat
    39481 tccttcaagt cagttgaaaa ctagtcttgc agcccttcgt aaagataaac ctgtccttct
    39541 ctacgagaac caacgtgcgc aacgagttac aaatgcagct ctttacttga aaaaacaagg
    39601 tttttctgag atttatatcc tttcttatgg cttggattct tggaaaggga aagtgaagac
    39661 tagctaacct tatttccaaa ttagtcgaat gataggatga agctattgtt ttatagtata
    39721 attaatttgt ataaaattta aagagttctt agaacatgaa agaaaagaag aaaaatccac
    39781 tttttgtggg tatcttgtcg attattcttg gtttgttatt tccaattgta ggcctgattt
    39841 tgggtatcat aggattggtc ttggctattt cataccaaaa agagtcacaa ttagagtata
    39901 aaatagaaaa gattcttaat atcttaggaa ttgtgatttc tgtagttaac tggattgtag
    39961 caatcgcctt aatttttcgg taaataaaag aaaaaagctt tagttttaaa gctttttttg
    40021 ttttatcgac tcatctctaa atagttttta atgatttgta actctttttg gttcaaaggg
    40081 cggtagttac cttctgctaa atctgggttc aatgtgaagt ccccaaattg gattcttttg
    40141 aggctagtca ccttaacacc aaccgagagg aacattttct tgatttgatg aaattttcct
    40201 tctgaaatgg tgatagaggc ttgactgagg gagggacttg cagatagaat ctctagtttt
    40261 gcgggtttac agacagtacc atctaaaaag acaattccct tttgaaaggc ttggatatgg
    40321 tcaggtgtta gaagtccatt aacctcaact tgataagtct tatcgacatg atattgggga
    40381 tggaggagtt ggaagcccaa gggaccgtta tcggtcaaga gaaggagtcc tgttgtatct
    40441 cggtccagtc ggccgacggc atagagcttg tcagactgga tgtcagatgg aagcaagtcc
    40501 atgacggtcg gaagttcctt gtctttgttg gctgtaacga caccagcagg tttatgaagc
    40561 ataagatagg tgtgttcata gccttgaatg attcgatcct gaaaaaggag ttcttgtagt
    40621 cctgtatcga tattttgagc tagggagcgg gctgggcaac catcgactag aatttcccct
    40681 ctgaggagtg cttgcttcat ggcctttcgg ctaacttttt cttgggctaa taaattatct
    40741 aaacgcatac tgtgcctatc ttatcataag tcgcttgctt tgaaaaggct tgacgtgaaa
    40801 agaaaagcgg agtgaaaaca tttacacttg cttgagttat gttatttatt tgaaattatg
    40861 gtataatcgt tcagttagaa aataaatttt gaatattata gaggaaatca tgacaaaatt
    40921 aagagaagat atccgtaaca ttgcgattat cgcccacgtt gaccacggta aaacaaccct
    40981 ggttgacgaa ttattgaaac aatcagaaac gcttgatgca cgtactgaat tggcagagcg
    41041 tgctatggac tcaaacgata tcgaaaaaga gcgtgggatt accatccttg ctaaaaatac
    41101 tgccgttgct tacaacggaa ctcgtatcaa cattatggac acaccaggac acgcggactt
    41161 tggtggagaa gttgagcgta tcatgaaaat ggttgacggt gttgtcttgg tcgtagatgc
    41221 ctatgaagga accatgccac aaactcgttt cgtattgaaa aaagccttgg aacaagacct
    41281 tgtcccaatc gtggttgtta acaaaatcga taagccatca gctcgtccag cagaagtagt
    41341 ggatgaagtc ttggaacttt tcatcgagct tggtgcagat gacgaccagc ttgatttccc
    41401 agtggtttat gcttcagcga tcaacggaac ttcttcattg tcagatgatc cagctgacca
    41461 agaagcgact atggcaccaa tctttgacac gattatcgac catatcccag ctccagtaga
    41521 taactcagat gagcctttgc agttccaagt gtcacttttg gactacaatg acttcgttgg
    41581 acgtatcggt atcggtcgtg tcttccgtgg tacagttaag gttggggacc aagttaccct
    41641 ttctaaactt gacggtacaa ctaaaaactt ccgtgttaca aaactcttcg gtttctttgg
    41701 tttggaacgt cgtgaaatcc aagaagccaa agcgggtgac ttgattgccg tttcaggtat
    41761 ggaagacatc tttgtcggtg aaaccatcac tccgacagat gcagtagaag ctcttccaat
    41821 cctacacatc gatgagccaa ctcttcaaat gactttcttg gtcaacaact caccatttgc
    41881 tggtaaagaa ggtaaatggg taacttctcg taaggtggaa gaacgcttgc aggcagaatt
    41941 gcaaacagac gtttcccttc gtgttgaccc aactgattca ccagataaat ggactgtttc
    42001 aggacgtgga gaattgcact tgtcaatcct tatcgaaaca atgcgtcgtg agggctatga
    42061 acttcaagta tctcgtccag aagttatcgt aaaagaaatc gacggtgtta aatgtgaacc
    42121 atttgaacgt gtacaaatcg acactccaga agaataccaa gggtctgtta tccaaagcct
    42181 ttctgaacgt aaaggtgaaa tgttggatat gatttcaact ggtaatggtc aaactcgttt
    42241 ggtcttcctt gttccagcgc gtggtttgat tggatactca actgagttct tatcaatgac
    42301 tcgtggttac ggtatcatga accatacctt cgaccaatac ttgccattga ttccagggga
    42361 aattggtgga cgtcaccgtg gtgcccttgt ttctatcgat gctggtaagg ctacaactta
    42421 ctcaatcatg tctatcgaag aacgtggtac gatctttgtc aacccaggta ctgaggttta
    42481 tgaaggaatg atcatcggtg aaaactctcg tgaaaatgac ttgacagtta acatcacgaa
    42541 ggcaaaacaa atgaccaacg ttcgttcagc tactaaggac caaacagctg ttatcaagac
    42601 gcctcgtatc ttgacacttg aagagtctct tgagttcttg aacgacgatg agtacatgga
    42661 agtaacgcct gagtctatcc gtttgcgtaa acaaatcctt aacaaagcag agcgtgagaa
    42721 agctaacaag aagaaaaaat cagctgaata agagctagaa agagagaaag atggtctatt
    42781 tagtcctagg aattttactg ctcctactct atgtatttgc gacaccagaa agcattaaag
    42841 ggactgtcaa tatcgtcgct atggtatgta ttttagtggc actcttgatt ttattggttc
    42901 tatcttttct gaaaattttt caattaccaa cagaaatatt cctagcaata gccatgttga
    42961 tcctagctta ctttagtgtt agagacatca cactcatgcc agtcaaaaaa agtaaaagaa
    43021 gataaaaaag agagtttcgg ctctcttttt gctaggaatt ttacaatagt ttgaattgct
    43081 ttttagttaa caccatctct tttacttgta tagtatgaat gggaaaaact tgaataaaat
    43141 tcgttaatgg catctatttt atatttggtt agta
//
LOCUS       DAVLXF010000005        40286 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLXF010000005 DAVLXF010000000
VERSION     DAVLXF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.005"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..476)
                     /locus_tag="VYO12_000269"
     CDS             complement(<1..476)
                     /locus_tag="VYO12_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586934.1"
                     /translation="MTQFQVRISGEAAYYLRMLKEKYETSEGINITKATILTRAFQNS
                     KTVTNWTKVVQDNSISLKNIYPVEEKELKLNVALSDEVAEGIKKYKEILPSATNTQSV
                     TLGVCVKFLLKAELMTQLNHDISEPEKDFDKKFSILEKQLLEIVAPVNHKLLSDTI"
     gene            905..2182
                     /locus_tag="VYO12_000270"
     CDS             905..2182
                     /locus_tag="VYO12_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1586935.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKIDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFSLNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2258..3742
                     /locus_tag="VYO12_000271"
     CDS             2258..3742
                     /locus_tag="VYO12_000271"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1586936.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGETVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNMQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTTVAAADMQVAVEAYLGL"
     gene            3803..5083
                     /locus_tag="VYO12_000272"
     CDS             3803..5083
                     /locus_tag="VYO12_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1586937.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5087..5707
                     /locus_tag="VYO12_000273"
     CDS             5087..5707
                     /locus_tag="VYO12_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1586938.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLDPEKTY
                     YIGDRTLDVEFAKNSGIQSINFLESSFEGNHRIQALADISRIFETK"
     gene            5873..7051
                     /locus_tag="VYO12_000274"
     CDS             5873..7051
                     /locus_tag="VYO12_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1586939.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASNSEVAETV
                     TAAEEVAPSTGTSVPEEQTAETTRPVEEVAPQATTLAEKQETQVSSQTESAVEATTMP
                     VEEKATETTATSSEAKEVASSNGATAAVSTYQPEETKTISTTYEAPAAPDYAGLAVAK
                     SENAGLQPQTAAFKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIA
                     EYAIQNMASRGISYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG
                     "
     gene            7564..7902
                     /locus_tag="VYO12_000275"
     CDS             7564..7902
                     /locus_tag="VYO12_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1586940.1"
                     /translation="MTRYQILLFLLEHSLCNQMAVQERLKIDQAALTRNFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            7913..8281
                     /locus_tag="VYO12_000276"
     CDS             7913..8281
                     /locus_tag="VYO12_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1586941.1"
                     /translation="MSIITIILTTIVAWEHFYIFYLESIATQSDATSRVFNMEREELA
                     HPSVSLLFKNQGIYKALLGVCLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            8410..9006
                     /locus_tag="VYO12_000277"
     CDS             8410..9006
                     /locus_tag="VYO12_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1586942.1"
                     /translation="MSPFEAIKGLPAEIHGAEVRWLEVPTVFHKSAQVLEEEMNRYQP
                     DFVLCIGQAGGRTSLTPERVAINQDDARTSDNEDNQPIDRPIRPDGASAYFSSLPIKA
                     MVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVKAGFMHIPYMMEQVVNR
                     PTTPAMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(9034..9891)
                     /locus_tag="VYO12_000278"
     CDS             complement(9034..9891)
                     /locus_tag="VYO12_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1586943.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10021..11163
                     /gene="tgt"
                     /locus_tag="VYO12_000279"
     CDS             10021..11163
                     /gene="tgt"
                     /locus_tag="VYO12_000279"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1586944.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11264..13081
                     /locus_tag="VYO12_000280"
     CDS             11264..13081
                     /locus_tag="VYO12_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1586945.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13234..14385
                     /gene="nagA"
                     /locus_tag="VYO12_000281"
     CDS             13234..14385
                     /gene="nagA"
                     /locus_tag="VYO12_000281"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1586946.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14548..15606
                     /locus_tag="VYO12_000282"
     CDS             14548..15606
                     /locus_tag="VYO12_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1586947.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15757..16122
                     /locus_tag="VYO12_000283"
     CDS             15757..16122
                     /locus_tag="VYO12_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1586948.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16198..17139
                     /locus_tag="VYO12_000284"
     CDS             16198..17139
                     /locus_tag="VYO12_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1586949.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17087..18103
                     /locus_tag="VYO12_000285"
     CDS             17087..18103
                     /locus_tag="VYO12_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1586950.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18105..18431
                     /gene="comGC"
                     /locus_tag="VYO12_000286"
     CDS             18105..18431
                     /gene="comGC"
                     /locus_tag="VYO12_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV1586951.1"
                     /translation="MKKMMTFLKKAKVKAFTLIEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18424..18828
                     /locus_tag="VYO12_000287"
     CDS             18424..18828
                     /locus_tag="VYO12_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1586952.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18833..19093
                     /locus_tag="VYO12_000288"
     CDS             18833..19093
                     /locus_tag="VYO12_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586953.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19056..19517
                     /locus_tag="VYO12_000289"
     CDS             19056..19517
                     /locus_tag="VYO12_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1586954.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19495..19941
                     /locus_tag="VYO12_000290"
     CDS             19495..19941
                     /locus_tag="VYO12_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1586955.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            20044..20638
                     /locus_tag="VYO12_000291"
                     /pseudo
     CDS             20044..20638
                     /locus_tag="VYO12_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20699..21652
                     /locus_tag="VYO12_000292"
     CDS             20699..21652
                     /locus_tag="VYO12_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1586956.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFTEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21703..22893
                     /locus_tag="VYO12_000293"
     CDS             21703..22893
                     /locus_tag="VYO12_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1586957.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRLDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADVIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23042..23413
                     /gene="rnpA"
                     /locus_tag="VYO12_000294"
     CDS             23042..23413
                     /gene="rnpA"
                     /locus_tag="VYO12_000294"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1586958.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23388..24212
                     /locus_tag="VYO12_000295"
     CDS             23388..24212
                     /locus_tag="VYO12_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1586959.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24231..25217
                     /locus_tag="VYO12_000296"
     CDS             24231..25217
                     /locus_tag="VYO12_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1586960.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25268..25891
                     /locus_tag="VYO12_000297"
     CDS             25268..25891
                     /locus_tag="VYO12_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1586961.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26022..27479
                     /locus_tag="VYO12_000298"
     CDS             26022..27479
                     /locus_tag="VYO12_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1586962.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27502..27783
                     /locus_tag="VYO12_000299"
     CDS             27502..27783
                     /locus_tag="VYO12_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1586963.1"
                     /translation="MVKVLSACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27860..28345
                     /locus_tag="VYO12_000300"
     CDS             27860..28345
                     /locus_tag="VYO12_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1586964.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28362..29027
                     /locus_tag="VYO12_000301"
     CDS             28362..29027
                     /locus_tag="VYO12_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1586965.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATISTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDIFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29031..29894
                     /locus_tag="VYO12_000302"
     CDS             29031..29894
                     /locus_tag="VYO12_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1586966.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29896..30600
                     /locus_tag="VYO12_000303"
     CDS             29896..30600
                     /locus_tag="VYO12_000303"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1586967.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30779..32452
                     /locus_tag="VYO12_000304"
     CDS             30779..32452
                     /locus_tag="VYO12_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1586968.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32564..33655
                     /gene="ulaG"
                     /locus_tag="VYO12_000305"
     CDS             32564..33655
                     /gene="ulaG"
                     /locus_tag="VYO12_000305"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1586969.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33769..35745
                     /gene="tkt"
                     /locus_tag="VYO12_000306"
     CDS             33769..35745
                     /gene="tkt"
                     /locus_tag="VYO12_000306"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1586970.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGINTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35862..36161
                     /gene="yajC"
                     /locus_tag="VYO12_000307"
     CDS             35862..36161
                     /gene="yajC"
                     /locus_tag="VYO12_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1586971.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36208..36636
                     /locus_tag="VYO12_000308"
     CDS             36208..36636
                     /locus_tag="VYO12_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1586972.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36638..37048
                     /locus_tag="VYO12_000309"
     CDS             36638..37048
                     /locus_tag="VYO12_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586973.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37348..39999
                     /gene="adhE"
                     /locus_tag="VYO12_000310"
                     /gene_synonym="adhC"
     CDS             37348..39999
                     /gene="adhE"
                     /locus_tag="VYO12_000310"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1586974.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSVHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFRPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12315 a         7152 c         8961 g        11858 t
ORIGIN      
        1 catatggtat cagaaagtaa tttgtggttg actggtgcaa cgatttcaag aagctgtttt
       61 tctagaatag agaatttctt gtcaaaatct ttttcaggtt ctgaaatatc gtgatttaat
      121 tgggtcatca attctgcttt aagaagaaat ttaacacata cacctaaagt tacactttga
      181 gtatttgtgg cactaggcaa tatttcttta tattttttaa ttccttctgc tacttcatcg
      241 gacagtgcaa cgttcaattt taattccttt tcttccactg gataaatatt ttttaaagag
      301 atagagttat cttgtacaac ttttgtccag tttgttacag tctttgaatt ttgaaacgca
      361 cgcgttaaaa tggtagcttt agttatattg ataccttctg aagtttcata tttctctttt
      421 agcattctta aatagtaagc tgcttcacca gatattcgga cttgaaattg tgtcattatt
      481 agtacctccg tattgtatta taccatttgt acattaaaag gacaatgtat aaacgaataa
      541 tctaccttct cgttcaagca tttttacaag tttagtaatc ttgttgaaga tgcattccat
      601 gtgttacgtt taaaaaaggg aattaaagta atctagttcc taaaaggtta ctaaaaataa
      661 aaaaactatc gtttttgtga tagaggtaac tttttgaaaa ggctatttga ctggctttat
      721 atacttatga aatgttaaga gcccaactca aaatccagtg tccgcaagga cgtgccggtt
      781 cgaccccggc cgccggtata gtatagtgtt aggaacgttg ttattcttcg ttcctttttt
      841 atattatttt tggtataatt atagttattc aaattttatt tagattaaga aagtgtaggg
      901 gagtatgtct tgttctatcg atttattaaa acatcggtat ttgaaaaata ttaaagaaaa
      961 tcctgaattg tttgtcggaa ttgagttgga gtatcctgtt gcaagtttag aaggggatgc
     1021 tacagatgtt gaagttataa aggatctatt tcattattta gtttctactt tggatctcac
     1081 cgtagcaaag atagatgatt ttggcaatct gatccagtta gtagatccga taagtcagga
     1141 tgctatttta tttgaagttt cctatacaac gattgagttt gcatttggta aggctgaaac
     1201 gattcaagag gtcgaaaatc gtttcaataa ttatatgaat gtaattcaga gaaagttagc
     1261 tgaatcaaat catgctattg ttggctgtgg tatccatccc aactgggata aaaatgagaa
     1321 ttgtccagtg gcttatccac gctatcagat gttgatggat tatttgaatt tgagtagaaa
     1381 tattattaaa tcagatttac atcatttccc tgaatatggt acttttatct gtgggagcca
     1441 ggttcagctg gatatttcaa aaaccaacta cttacgggtg attaatgctt ttactcaaat
     1501 tgaagcggct aaggcttatt tatttgcaaa ctctgagttt tcgggtgcgg attgggatac
     1561 gaaaatttca agggatattt tctgggaaga atctatgcat ggtatctatc cagagaatgt
     1621 tggggtcaat gctagactcc ttaatgatga agctgatttt tttgactatc taaatcattc
     1681 tgcgattttt actgcggaac gtgatgggca gacctattat ttttatccta ttcaggctgg
     1741 ggactatttg gctacgcctg aaatccaagc attttctctg aatggggatg aggttattat
     1801 ttacccccaa gagaaggatt ttgaaactca tcgtagttac cagtaccaag acttaacgac
     1861 tcgaggaaca gttgagtttc gtagtgtgtg tacacagcca cttgatagaa cttttgcttc
     1921 tgcagctttt cacttgggat tattggttaa tttagacaag ttagaagctt acttagaaac
     1981 agcacctttc tttaaagtat ttggttatga ttacaagtct ttaaggagac aattttctaa
     2041 gaaaaatctt acagatgagg aagaaactac gattattgaa ttttccaaag acttactcct
     2101 actagctgag gagggactag tggtgagaaa taaggaagaa atgacctatt tacagccttt
     2161 gagagaagaa ttgagcctat aatttctctt ataaagggag aattttctga aaaatcatga
     2221 tataatggac gagactatag ataaaggata gagagtaatg acattagttt atcaatcaac
     2281 gcgtgatgcc aacaatacag taactgccag tcaagcaatt ttgcaaggtt tggcgacgga
     2341 cggcggtttg tttacaccgg ttacttatcc aaaggtagat ttggactttg acaaattgaa
     2401 agatgcttct taccaggaag ttgctaagct agttttgtca gcatttttag atgactttac
     2461 agctgaggag ttggactact gtatcaacaa tgcctacgat agcaaatttg atactccagc
     2521 tattgcacca ttagtgaaat tagatgggca atacaatttg gaacttttcc atggttcaac
     2581 gattgccttt aaggatatgg ccttgtctat tttgccatac tttatgacga ctgctgctaa
     2641 gaaacatggt ttggagaaca agattgttat cttgacagcg acatctggtg acacggggaa
     2701 agctgctatg gcggggtttg cggatgtgcc tggtactgag attatcgtct tttatccaaa
     2761 ggatggtgtc agcaagattc aagagttaca aatgaccact cagactggcg acaatactca
     2821 tgttattgct attgatggta actttgacga tgcgcaaaca aatgtgaagc acatgtttaa
     2881 cgatgtggct cttcgtgaaa aattgactac caacaagttg caattttcat cagctaactc
     2941 tatgaacatt ggtcgtctgg tgccacaaat tgtttattat gtttatgctt acgctcaatt
     3001 ggttaagact ggtgaaactg tagctggtga aaaggttaac ttcacagtac caacaggaaa
     3061 ctttggaaat atcttggctg ccttttatgc caaacaaatt ggtttgccag ttggtaaatt
     3121 gatctgtgct tcaaatgaca acaatgtttt gacagacttc tttaaaacac gtgtctatga
     3181 caaaaaacgt gagtttaagg taacaaccag cccatctatg gatatcttgg tatcttcaaa
     3241 cttggagcgc ttgattttcc atcttttggg aaataatgct gaaaagacag ctgaactcat
     3301 gaatgctttg aacatgcaag gacaatataa gttgacagac tttgatgcag agattttgga
     3361 cctctttgca gctgaatatg cgactgagga agaaacagcg gcagaaatca agcgtgttta
     3421 tgagttagat tcttatattg aggatccaca tacggcggtt gcctcagcag tttatagaaa
     3481 ataccaagcg gttactgatg atgcgactaa gacagtgatt gcttcaacag ctagtcccta
     3541 caagttccca gtagttgcag tagaagctgt aactggaaaa gcaggtttaa cagactttga
     3601 agctttggct caattacatg aaatctcagg cgttgcagtg ccaccagcag ttgatgggct
     3661 tgagacagct cctgttcgtc acaagacaac agtggcagct gctgacatgc aagtagcggt
     3721 tgaggcttat ttaggacttt aagatagagg gagtaaactc ggttgggaaa ccaactgagt
     3781 ttcttttcat caggaggaga gattgtttaa gaaaaataaa gacattctta atattgcatt
     3841 gccagctatg ggtgaaaact ttttgcagat gctaatggga atggtggaca gttatttggt
     3901 tgctcattta ggattgatag ctatttcagg ggtttcggta gctggtaata ttatcaccat
     3961 ttatcaggcg attttcatcg ctctgggagc tgctatttcc agtgttattt caaaaagcat
     4021 agggcagaaa gaccagtcga agttggccta tcatgtgact gaggcgttga agattacctt
     4081 actattaagt ttccttttag gatttttgtc catcttcgct gggaaagaga tgataggact
     4141 tttggggacg gagagggatg tagctgagag tggtggactg tatctatctt tggtaggcgg
     4201 atcgattgtt ctcttaggtt taatgactag tctgggagcc ttgattcgtg caacgcataa
     4261 tccacgtctg cctctctatg ttagtttttt atccaatgcc ttgaatattc ttttttcaag
     4321 tctagctatt tttgttctgg atatggggat agctggtgtt gcttggggaa caattctgtc
     4381 tcgtttggtt ggtcttgtga ttttgtggtc acaattaaaa ctgccttatg ggaagccaac
     4441 ttttggttta gataaggaac tgttgacctt ggctttacca gcagctggag agcgacttat
     4501 gatgagggct ggagatgtag tgatcattgc cttggtcgtt tcttttggga cggaggcagt
     4561 tgctgggaat gcaatcggag aagtcttgac ccagtttaac tatatgcctg cctttggtgt
     4621 cgctacggca acggtcatgc tgttggcccg agcagttgga gaggatgatt ggaaaagagt
     4681 tgctagtttg agtaaacaaa ccttttggct ttctctgttc ctcatgttgc ccctgtcctt
     4741 tagtatatat gtcttgggtg taccattaac tcatctctat acgactgatt ctctagcggt
     4801 ggaggctagt gttctagtga cactgttttc actacttggg acccctatga cgacaggaac
     4861 agtcatctat acggcagtct ggcagggatt aggaaatgca cgcctccctt tttatgcgac
     4921 aagtataggc atgtggtgta tccgcattgg gacaggatat ctgatgggga ttgtgcttgg
     4981 ttggggcttg cctggtattt gggcagggtc tctcttggat aatggttttc gctggttatt
     5041 tctacgctat cgttaccagc gctatatgag cttgaaagga taggaaatgc aaaaaacagc
     5101 ttttatttgg gatttagacg ggactttatt ggactcttac gaagcgattt tatcagggat
     5161 tgaggagact tttgctcagt tttctattcc ttatgataag gagaaggtga gagagtttat
     5221 cttcaagtat tcggtgcaaa atttgcttgt gcgggtggca gaagatagaa atctggatgt
     5281 tgaggtgcta aatcaggtgc gtgcccagag tctggctgag aagaatgctc aggtagtttt
     5341 gatgccaggt gcgcgtgagg tgctagcttg ggcagacgaa tcaggaattc agcagtttat
     5401 atatactcat aaggggaaca acgcttttac cattctcaag gacttggggg tggaatccta
     5461 ttttacagag attttaacca gtcagagtgg ctttgtgcgg aagccaagtc cagaagcggc
     5521 tacctatctg ctagataagt atcagctgga tcctgagaag acctattata taggggaccg
     5581 gactttagat gtggaatttg ccaagaatag cggtattcaa agcatcaact ttttagaatc
     5641 ttcttttgaa ggcaatcaca ggattcaagc actagcagat atttcccgta tttttgagac
     5701 taagtgataa aaagattgtg tcagttttgt gacagagacc taacaaacta tttcaagtaa
     5761 tctagtttgt tacaaggaat agacagttct gttaaatagg cccgagaggg ctttttttct
     5821 acattttttg tgttatgata gacaggtact catttgaaag gaatttgaaa gaatgaagaa
     5881 aagaatgtta ttagcgtcaa cagtagcctt gtcatttgcc ccagtattgg caactcaagc
     5941 agaagaagtt ctttggactg cacgtagtgt tgagcaaatc caaaacgatt tgactaaaac
     6001 ggacaacaaa acaagttata ccgtacagta tggtgatact ttgagcacca ttgcagaagc
     6061 cttgggtgta gatgtcacag tgcttgcgaa tctgaacaaa atcactaata tggacttgat
     6121 tttcccagaa actgttttga caacgactgt caatgaagca gaagaagtaa cagaagttga
     6181 aatccaaaca cctcaagcag actctagtga agaagtgaca actgcgacag cagatttgac
     6241 aaccaatcaa gtgaccgttg atgatcaaac tgttcaagtt gcagatcttt ctcaaccaat
     6301 tgcagaagct ccaaaagaag tagcatcaaa ttcagaagtt gcagagacag tgactgctgc
     6361 agaagaagtg gcaccatcta cgggcacttc tgtcccagag gagcaaacgg ccgaaacaac
     6421 tcgcccagtt gaagaagtag ctcctcaggc aacgactcta gctgagaagc aggaaacaca
     6481 agtgagctct caaaccgagt cagcagtgga agcaactaca atgccagtag aagaaaaagc
     6541 aaccgaaaca actgcaacaa gttcagaagc aaaagaagta gcatcatcaa atggagctac
     6601 agcagcagtt tctacttatc aaccagaaga gacgaaaaca atttcaacaa cttacgaggc
     6661 tccagctgcg cccgattatg ctggactggc agtagcgaaa tctgaaaatg caggtcttca
     6721 accacaaaca gctgccttta aagaagaaat tgctaacttg tttggcatta catcctttag
     6781 tggttatcgt ccaggagaca gtggagatca cggaaaaggt ttggctatcg actttatggt
     6841 accagaaagt tcagaattag gggataagat tgcggaatat gctattcaaa acatggccag
     6901 ccgtggcatt agttacatca tctggaaaca acgtttctat gctccattcg atagcaaata
     6961 tgggccagct aacacttgga acccaatgcc agaccgtggt agtgtgacag aaaatcacta
     7021 tgaccacgtt cacgtttcaa tgaatggata aacccgactt ggtaacatca ttttgacgaa
     7081 tgagatctag ctttcgtgat aggaagcgat tctcgttcgt tttttctttg tcatactctt
     7141 cgaaaatctc ttcaaacaac gtcagtttta tctgaaacct caaagctgtg ctttgagcaa
     7201 cctgcgacta gcttcctagt ttgctttttg attttcattg agtatcaatt tgaatggaaa
     7261 atggaaagtt atcatcttgt aatgagttaa gcaacattct tgcaatctat tttatagtag
     7321 attgaaacta gaatagtaca caactgcttc taaaacattg ttagaaatcg atttgactgt
     7381 cctgatcgat ttgtcctgct cttatttcat ttcactatac tttatattat aattgactgg
     7441 tcaaatattg ataaatcaat aaaaagagag gggaagaaat gctagagatt caagatttac
     7501 tgtatcaact ccgcttgtct gagcaagtga gtacgcaatt gtttgaaaaa gcttgggatt
     7561 agtttgacac ggtatcagat tttactgttt ttgctggagc attctctttg taaccaaatg
     7621 gcggttcagg agcgtttgaa aattgatcag gctgctttga cacggaattt caagattttg
     7681 gaaacggaag gtttggtgga gcgtcatcgt aatcctgaaa atcagcggga agtattggta
     7741 gaggctgcga agtatgccaa ggagcagtta gtggtgaatc cccctctgca acatatcaag
     7801 gttaaggaag agatggaaag tatcttaaca gagtttgaga gaacagaact cagccgttta
     7861 ttaaataaat tggttttggg tattgaaaat atagaaattt aaggagaaat agatgtcaat
     7921 tattacaatt attttaacaa cgatcgttgc ttgggagcat ttttacattt tttatttaga
     7981 aagtattgcc acgcaatcag atgcgactag tcgtgtattt aatatggaaa gggaagaatt
     8041 ggctcatccg tcagtaagtt tattgttcaa aaatcaaggg atttataagg ctctgctagg
     8101 agtctgtctc ttgtatggca tttatttctc acagaattta gaaattgtga ctatttttgt
     8161 cttatttgtg attggtgctg cgacttacgg ctctttaaca gcggataaaa aaattatttt
     8221 gaaacaaggt ggatcagcta ttttggcctt gattagtatt ttactcttta aatacacttg
     8281 aaggtcgatt ctaatctcgc taatcctttt taatccagaa taagggaaat atgttatact
     8341 tgtttttaag aaaaaagttt cattgaattg gttttgagga gttagaaatg aaagtattag
     8401 tgacaggttt tgagcccttt tgaggccatt aaaggtttac cagctgaaat ccatggtgct
     8461 gaggtccgtt ggctagaggt gccgacagtt tttcacaaat ctgctcaagt attggaagaa
     8521 gagatgaatc gttatcaacc tgactttgtc ctttgtattg ggcaagctgg tggaagaact
     8581 agtttgacac ctgaacgagt ggccattaat caagacgatg cacgtacttc tgataacgaa
     8641 gataatcaac cgattgaccg tcccattcgc ccagatggtg cttcggccta ctttagtagt
     8701 ttgccgatta aagcgatggt tcaagctata aaaaaagagg gcttaccggc ctctgtttcc
     8761 aatacggcag ggacttttgt ctgcagccat ttgatgtatc aggctctcta tttggtagaa
     8821 aagaaatttc catatgttaa ggcaggtttt atgcatattc cttatatgat ggaacaggtg
     8881 gtgaacagac cgactactcc agctatgagt ttagtggata ttcggcgagg gatagaagca
     8941 gcaatcggcg ctatgataga acatggagat caggaactca agttggtagg cggagaaact
     9001 cattgataga aaaaagcttg aggggaaaaa ccttcaagct tttggacgtt ttcgagccaa
     9061 tactgctcgg taaaacataa ttttagtgca ttggatataa ggtaggagtg aaaaactagc
     9121 aatgccaaag gtaatccaat tgaggaagta ccaaggaaga agctgtaaat ctaggacaaa
     9181 gcgctggaac ttgtagccct tcataaagga acggctagtt tttaggattc gtcttggtgg
     9241 gacctgtcct aggtctagac tataacagag aagaaattcc acctgtgaat aggcataata
     9301 ctgtggaata tagaggatat ttcctacaat gatcaagatg agacttgcaa gaaagtagag
     9361 tccaaagacc atgaggaaac gctcggtttc aactgatgag agatctagat ttggaaactc
     9421 aggatgtagg gtgacgaatt ttttggctaa aaagctacta taaaagagga ggtaaatccc
     9481 aagtaaatta gggatactcc ataaaaagag atagaaacgt ttgagaagta gggtcaaaaa
     9541 ggtttgagaa aagcgctcct catcaaagag agctaggctg ttttttacag atggctccgt
     9601 tttagaatct ttcatgagtg tcagtgttgc atagacggaa ctggtcaaaa gaatagtccc
     9661 gataaaggag actagtagag gaaagaggta ggtttgaagt atttggccaa gtatgctgaa
     9721 aaatggctgt tctaaaacag tcccgtggat ccgagataag ggattaagaa aactagataa
     9781 gatgaccagc atactgggaa ggatatagag gagaaagaga cggggggtgt cagcctgaaa
     9841 atgttttgac tcctgacgaa ttgtttttaa atcaattttt ggatagttca ttctcttatt
     9901 ataccatagt tcttatacat agttcgtgac agttcctact ttttttgata aaatcataca
     9961 gtgtgtcctt gggcacactg tatgaactgg gactgtcttt cccagcttcg gaggtaaaaa
    10021 atgtcagatt caccaatcaa atatcgtttg attaagaaag aaaaacacac aggagctcgt
    10081 ctaggagaaa tcatcactcc ccacggtacc tttccgacac ctatgtttat gccagttggg
    10141 acacaagcca ctgtcaaaac tcagtcacct gaagaattga aggagatggg ttcgggaatt
    10201 atcctatcaa acacctatca tctctggctt cgccctggag atgaactcat tgcacgcgct
    10261 ggtggtctcc acaagttcat gaattgggac cagcctatct tgacagatag tggtggtttt
    10321 caggtttatt ctttagcaga tagccgtaat atcacagaag aaggagtaac ctttaaaaat
    10381 catctaaatg gttctaagat gctcctatcc ccagaaaaag ccatctctat tcagaataat
    10441 ctgggttcag acatcatgat gtcctttgat gaatgtcctc agttttatca accttatgac
    10501 tacgttaaga aatcgatcga gcgtaccagc cgttgggctg agcgtggttt gaaggctcac
    10561 cgtcgtccac atgaccaagg tttgtttgga attgtgcaag gtgcaggatt tgaagacctt
    10621 cgccgccaat cagctcatga tcttgtcagc atggatttct caggctactc tatcggtggt
    10681 ttggcagtgg gagaaaccca tgaagagatg aatgcggtct tggactttac aactcaactg
    10741 ctgcctgaaa ataaacctcg ttatctgatg ggtgtgggag cgccagatag cttgatcgat
    10801 ggggtcattc gtggggtgga tatgtttgac tgtgtcttac cgactcgaat tgctcgtaac
    10861 gggacttgta tgaccagtca aggacgtttg gttgtgaaaa atgcccagtt tgctgaggac
    10921 tttacgccac tggatcctga gtgtgattgc tacacatgta ataactatac acgcgcttac
    10981 cttcgtcacc tgctcaaggc tgatgaaacc tttggtatcc gcttgactag ctaccacaat
    11041 ctttacttct tgcttaacct gatgaagcaa gtgcgacaag ccatcatgga tgacaatctc
    11101 ttggaattcc gtaagtattt tgtggaaaaa tatggctata ataagtcagg acgtaatttc
    11161 taaaatggaa ttgatataag ctaaaaatcc taagttttct cttaggattt ttcttctttt
    11221 tttgatagaa taaagtgtac aatgaaagga agaataaact cgtatgcgca ttaaatggtt
    11281 ttccttggtt aggattacag gtttactttt ggtactcttg tatcacttct ttcagacgat
    11341 ctttcctgga ggatttttcg gggtagatgt ctttttcaca ttttcaggtt tcctgattac
    11401 agctctactc attgaagaat tttctaaaaa ccatgagatt gatttgatag gattttttag
    11461 aagacgcttt tatcggattg tgccacctgt ggttttgatg gtcttggtga ccatgccttt
    11521 tactttcttg gttcgccaag actatgttgc tggaattggt ggccagattg cgagcgtctt
    11581 aggctttatg accaacttct atgaactcct aacaggtggg agttatgaat ctcagttcat
    11641 tcctcatttg tttgttcata attggagttt ggcagttgag gttcactact atattctttg
    11701 gggattggca gtttggttct tatccaaaca agctaaatca aatggtcagt tgaaggggat
    11761 ggtctttctc ttatctgctg ttgccttctt gatcagtttc ttctccatgt ttattggtag
    11821 ttttctagtg acctcttatt cctctgttta tttctccagt ttaactcatg tctatccatt
    11881 ctttttggga agtatgttag caactattgt aggcgttcgt cagacgactt ccctcgtcaa
    11941 gcagttggat aaaatctggg atttacgaaa gactttggta gtttttggag gaggctttgg
    12001 tttcctagtt cttttgactt tctttgtcaa attcacttat ctttttgcct atcttatcgg
    12061 cttcttactt gccagtcttg cagctcttgc catgattctg gcggcgcgtg tcttacatga
    12121 aaagacacat catatacagg agccgaagat tatcagcttt ttagcggata ctagctatgc
    12181 ggtttatctt ttccattggc ctttctatat cattttctca cagttgacat caaatcttct
    12241 tgctgtatta ctgactctga tttgttctta tggctttgcc agtctgtcat tctatgtatt
    12301 ggaaccttgg attgcaggca agaacacacc tattgtccaa acccttcgtc ccctgcctta
    12361 tattcacgca attcttgcag caggtacagg aatcttgacc atcattgtct gcacggtgac
    12421 cttgttggca ccacaagtgg gagcgtttga gacagacttg actgtcaatg gcttgaagca
    12481 agctgcaaca aatattggcc agaccaaggt gatggcagaa cgggcagatg caaacagttt
    12541 gggaattgct gatggcacta tgttaattgg tgactcagtg gctttaaggg caaatacagc
    12601 actacagaca gctcttcctg gagcacagat taacgcgcag gtcagcgtaa caaccaagac
    12661 cgcaaatgaa atcatgctaa ataatagcca gaataaattt ttacctaaga cggtggtcat
    12721 tgcgactggg gtaaataatc ctgagaatta caaggatgac tgggacagta tcgtgaaaaa
    12781 tcttcctaag ggacaccata tgattttggt gactccttat gagggagata agacaaaaga
    12841 gacctatgcc atcgttgaga aggctgctgc ctatatgaga gaattggcag agaagacacc
    12901 ttacattacg atagcagatt ggaatcaagt tgcgaaagag catccagaaa tttgggcagg
    12961 aacagaccag gttcatttcg ggagtgagag tagcactatc gaagcaggag caaaattgta
    13021 tgcagatacg attgccacag ctttgcagac agctcaagac aagccggtta aatcaaaata
    13081 acttgtatta aaaaagagaa gagttggaga aatccagctc ttttaaaata tctccaaaaa
    13141 ataggtctat accatttaca aataaaaaag aaaggtttat aatgtaattg acataataaa
    13201 ttgtagaatc aatcttttaa ggaggttaac attatgccta actatattaa agcggatcag
    13261 tttttctacc cacacggagt tcgtcgaggt ggttacttgg aacttgtgga cggcaagttt
    13321 gggaaacatg tagagcagat tcctgaaggg gctgaggtga ttgactatac aggttatagc
    13381 attgccccag gtcttgtgga tactcatatt catggatatg caggtgtaga tgtgatggac
    13441 aacaacattg aaggtacatt gcatactatg agtgaaggac ttcttagtac cggtgttacc
    13501 agtttcttac ccacaacttt aacagccact tatgagcaat tgcttgcagt cactgaaaat
    13561 cttggaaacc attataaaga agcaacaggt gctaagattc gtgggattta ttatgaaggt
    13621 ccatatttca cagaaacttt taagggggca caaaatccaa cttatatgag agacccgggt
    13681 gttgaggagt ttcattcttg gcaaaaagcg gcaaatggct tgcttaataa aattgccctt
    13741 gcaccagaac gtgatggggt ggaggacttt gttcgtacag ttacgggcga aggtgtgacg
    13801 gttgctcttg gacattcaaa cgcgactttt gatgaagcca aaaaagcagt cgatgctgga
    13861 gcgagtgttt gggtgcatgc ctacaatgga atgcgtgggt tgactcaccg tgaattgggt
    13921 atggttggag ccatgtacca attgccacat acctatgcag agttgatctg tgatggccac
    13981 cacgtagatc caaaggcttg cgaaattctt atcaaacaaa aaggaacaga aaacatcgct
    14041 cttatcacag actgtatgac agctggggga ttggaagacg gagattatat gttgggagaa
    14101 ttcccagttg tcgttgcaaa tggaactgca cgcctcaaat cgacaggtaa cttggcaggt
    14161 tctatcctca aactcaaaga tggtttgaag aatgtggtcg aatggggaat tgcgaatccg
    14221 catgaagcag tcatgatggc cagcttcaac ccagctaaat ccgttcacat cgatgatgtc
    14281 tgtggccaaa tccgtgaagg ctacgacgct gacttcatcg tattagataa agatttggaa
    14341 ttggtagcaa cctacctaga tggcgtaaaa cgttatcaag cataagagag aaagcagaag
    14401 ttagagacta acttctgctt ttttatgcag ggtactttac tggtaaataa aaagttaaaa
    14461 aaatcacaaa aaaagcttga agaaacaaag aaaaaggctt acaattgaac tataaatatt
    14521 acaaataaaa aaacggagga gtgctttatg aaagcctata cttatgttaa accaggactt
    14581 gcttcttttg ttgatgtaga caaaccagtt attcgcaagc caacagatgc tattgtgcgt
    14641 attgtaaaaa ccactatttg tggaacagac ctccatatta tcaaagggga tgttcctact
    14701 tgccaaagtg gtaccattct tggccacgaa gggattggga ttgttgaaga agttggggaa
    14761 ggagtttcca acttcaaaaa aggtgacaag gtcttgattt cttgcgtctg tgcctgtggt
    14821 aaatgctact actgtaaaaa aggaatttat gctcactgtg aagacgaagg gggctggatt
    14881 ttcggtcact tgattgatgg tatgcaggct gaatatctac gtgtccctca tgcagataat
    14941 actctttacc atactccaga agacttgtca gatgaagctt tggttatgct gtcagacatt
    15001 ctgcctactg gatatgaaat tggtgtctta aaagggaaag tagaacctgg ttgcagcgta
    15061 gccattattg gttcaggtcc agttggattg gctgctcttt taacagccca attctattca
    15121 ccagctaaat tgattatggt agacctagac gataaccgct tggaaactgc cctatcattc
    15181 ggtgcgactc ataaggttaa ttcttcagac cctgaaaaag ccattaaaga aatttatgat
    15241 ttgacagatg gtcgtggtgt ggatgtcgct atcgaagctg ttggtattcc tgcaacattt
    15301 gatttctgtc aaaagattat cggtgtagac ggaacggttg ccaactgtgg tgtgcatggt
    15361 aaaccagttg aattcgattt agataaactt tggattcgca acatcaatgt aacaactggt
    15421 ttggtatcta caaatacgac tccacaattg ttgaaagcac ttgaaagtca taagattgaa
    15481 ccagaaaaat tggtaactca ctatttcaaa ctcagtgaaa ttgaaaaagc ctatgaagtc
    15541 ttcagtaagg cagcagacca ccatgccatt aaggtcatta tcgaaaacga tatctcagaa
    15601 gcctaagtag taaaaatatt tttgtacata agtaaataga aattcagtca tccatcagat
    15661 ggctggattt tttatcaaaa aattaagaaa tgagcatatt tctttccttg tctggcggaa
    15721 ttggttataa tatacggtac aaaggaatga atgaatatgt atcgtgttat agaaatgtac
    15781 ggagattttg aaccgtggtg gttcttagaa ggttgggaag aagatattgt agcaagtaga
    15841 aaatttgacc agtattatga tgctctcaaa tactacaaaa cttgctggtt tagattggaa
    15901 caagaatccc ctctttataa aagtagaagc gacttgatga ccattttttg ggacccggaa
    15961 gaccaacgct ggtgtgatga atgtgatgag tatttacaac aataccattc tttggctctt
    16021 ttgcaggatg agcaggttat cccagacgaa aaactacgct caggctatga aaaacaaacc
    16081 agtcaggaaa ggaatcgttc ttgccgtatg aaattaaaat agagaaaagt aacttttttg
    16141 gagttgcttt ttttattttt ctaacgcttt gcgaatagta taggtgagga ggtaagtatg
    16201 gttcaagaaa ttgcacaaga aatcattcgt tcagctcgga aaaaagggac gcaggatatt
    16261 tattttgtcc ctaagttaga cgcctatgag cttcatatga gggtaggaga cgagcgctgt
    16321 aaaattggta gctatgattt tgaaaagttt gcagccgtta tcagtcactt taagtttgtg
    16381 gcgggtatga atgtgggaga aaaaagacgt agtcaactgg gttcctgtga ttatgcctat
    16441 gaccagaaga tggcgtctct acgtttatct actgtaggcg attatcgggg acatgagagt
    16501 ttggttatcc gtttgttgca cgatgaggag caggacttgc atttttggtt tcaggatatt
    16561 gaagaattag gcaagcagta caggcaacgg ggactctatc tttttgctgg cccggttggg
    16621 agtggtaaga cgaccttgat gcatgaattg tccaagtcac tctttaaagg acagcaagtt
    16681 atgtccatcg aagatcctgt cgaaatcaag caggacgaca tgcttcagtt gcagttgaac
    16741 gaagcaatcg gcctaaccta tgaaaatcta atcaaacttt ccttgcgtca tcgaccagat
    16801 ctcttgatta tcggagaaat tcgtgacatc gagacggcgc gtgcagtggt cagagctagt
    16861 ttgacaggtg cgacagtctt ttcaaccatt cacgccaaga gtatccgagg tgtttatgag
    16921 cgtctgctgg agttgggtgt gagtgaagaa gaattggcag ttgttctgca aggagtctgc
    16981 taccagagat taatcggggg aggaggaatc gttgactttg caaacagaga ttatcaagaa
    17041 caccaagcag ccaagtggaa tgagcaaatt gaccagcttc ttaaagatgg acatatcaca
    17101 agtcttcagg ctgagacgga aaaaattagc tacagctaag caaaaaaata tcatcaccct
    17161 atttaacaat ctcttttcta gcggttttca tctggtggag actatctcct ttttagatag
    17221 gagttccttg ttggacaagc agtgtgtgac ccagatgcgt acaggcttgt ctcaggggaa
    17281 atcattctca gaaatgatgg aaagtttggg atgttcaagt accattgtca ctcagttatc
    17341 tctagctgaa gttcatggca atcttcacct gagtttggga aagatagaag aatatctaga
    17401 caatctggcc aaggtcaaga aaaagttaat tgaagtagcg acctatcctt tgattttgct
    17461 gggttttctt ctcttaatta tgctggggct acggaattac ctgctcccac aactggatag
    17521 tagcaatatt gccacccgaa ttatcggtaa tctgccacaa atttttctag gcatggtagg
    17581 gcttgtttcc gtgcttgccc ttttagcact aactttttat aaaagaagtt ctaagatgag
    17641 tgtcttttct atcttagcac gccttccctt tattggaatc tttgtgcaga cctacttgac
    17701 agcctattat gcacgtgaat gggggaatat gatttcacag ggaatggagc tgacgcagat
    17761 ttttcaaatg atgcaggaac aaggttccca gctctttaaa gaaatcggtc aagatctggc
    17821 tcaaaccctg aaaaatggcc gtgaattttc tcagacgata ggaacctatc ctttctttag
    17881 gaaggaattg agtctcatca tagagtatgg ggaagttaag tccaagctgg gtagtgagtt
    17941 ggaaatctat gctgaaaaaa cttgggaagc cttttttacc cgagtcaacc gtaccatgaa
    18001 tttggtgcag ccactggttt ttatctttgt ggcactgatt atcgttttac tttatgcggc
    18061 aatgctcatg cccatgtatc aaaatatgga ggtaaatttt taaaatgaaa aaaatgatga
    18121 cattcttgaa aaaagctaag gttaaagctt ttacattgat tgagatgttg gtggtcttgc
    18181 tgattatcag cgtgcttttc ttgctctttg tacctaatct gaccaagcaa aaagaagcag
    18241 tcaatgacaa aggaaaagca gctgttgtta aggtggtgga aagccaggca gaactttata
    18301 gcttagaaaa gaatgaagat gctagcctaa gaaagttaca agcagatggg cgcatcacgg
    18361 aagaacaggt taaagcttat aatgaatact atactaaaaa tggaggggca aatcgtaaag
    18421 tcaatgatta aggcctttac catgctggaa agtctcttgg ttttgggact tgtgagtatc
    18481 cttgccttgg gcttatccgg ctctgtccag tccacttttg cagcagtaga ggagcagatt
    18541 ttctttatgg agtttgaaga actctatcgg gaaacccaaa aacgcagtgt agccagtcag
    18601 caaaagacta gtctaaattt agatgggcag acgcttagca atggcagtca aaagttgaca
    18661 gttcctaaag gaattcaggc accatcaggc caaagtatta catttgaccg agctgggggc
    18721 aattcgtccc tggctaaggt tgaatttcag accagtaaag gagcgattcg ctatcaatta
    18781 tatctaggaa atggaaaaat taaacgcatt aaggaaacaa aaaattaggg cagtgatttt
    18841 actggaagca gtagtcgctc tagctatctt tgccagcatt gcgaccctcc ttttgggaca
    18901 aattcaaaaa aataggcaag aggaagcaaa aatcttgcaa aaggaagaag tcttgagggt
    18961 agctaagatg gccttgcaga caggtcaaaa tcaggtaaac ataaacggag ttgagattca
    19021 ggtgttttct agcgaaaagg gattggaggt ctaccatggt tcagaacagt tgttggcaat
    19081 caaagagcca taaggtcaag gcttttacct tgttagaatc cctgcttgcc ctgattgtca
    19141 tcagtggggg cttactcctt tttcaagcta tgagtcagct cctcatttca gaagttcgct
    19201 accagcaaca aagcgagcaa aaggagtggc tcttgtttgt ggaccaactt gaggcagaat
    19261 tagaccgttc gcagttcgaa aaagtagaag gcaatcgcct atacatgaag caagatggca
    19321 aggacatcgc catcggtaag tcaaagtcag acgattttcg taaaaccgat gctagtggac
    19381 gggggtatca gcctatggtt tatggcctca aatcagctca gattacagag gacaatcaac
    19441 tggttcattt tcgtttccag tttcaaaaag gcttagaaag ggagttcatc tatcgtgtgg
    19501 aaaaagaaga aagttaaggc aggtgttctc ctctacgcag tcaccatagc agccatcttt
    19561 agtcttttgt tgcaatttta tttgaaccga caaatcgccc actatcaaga ctatgcttta
    19621 aataaagaaa aattggttgc ttttgccatg gctaaacgaa ccaaggataa agccgagcaa
    19681 gaaagtgggg aacaggtttt taatctaggt caggtaagct atcaaaacaa gaaaacaagc
    19741 ttggtgacga ctgttcgtac gtctaagagc caatatgagt ttctgtttcc ttcagtcaaa
    19801 atcaaagaag agaaaagaga taaaaaggaa gaggtagcga ccgattcaag cgaaaaagcg
    19861 gagaaaaaaa atcagaagag aagcctgaaa agaaagagaa ttcctagtca attcaactat
    19921 aatgcgttga atccagaata gtccactgta gtttctagaa aattgctgga aatggatgtt
    19981 aagctccaat tcatttgttt atatcttatt tcagtttact atactttgtg ctaaattaaa
    20041 gatatgaaac atgattttaa ccacaaagca gaaactttcg attcccctaa aaatatcttc
    20101 ctcgcaaact tggtatgtca agcagccgag aaacagattg atcttctatc agacaaagaa
    20161 attttagatt tcggtggtga cacgggtcta ttagccttgc ccctagcccc tagccaagca
    20221 ggctaagtca gtcactcttg tagacatttc tgagaaaatg ttggagtaag ctcgtttgaa
    20281 agcggagcag caagcaatca agaatatcca gtttttggag caagatttac cgaaaaatcc
    20341 cttggagaaa gagtttgatt gccttgctgt tagtcggctt cttcatcata tgcctgattt
    20401 ggatgcggat ctctcactgt ttcatcaaca tttgaaggaa gataggaaac tcatcattgc
    20461 tgattttacc aagacagaag ctaatcatca tggatttgat ttagctgaac tggaaaacaa
    20521 gctaattgag catggttttt catctgtgca tagtcagatt ctctatagcg ctgaagacct
    20581 gtttcaaggg aatcactcag aattcttttt aacagtatcc caaaaatcac tcgcctagtc
    20641 agggagtgat ttttctataa ggatggaaaa aagaagggaa atttgataag ataggaatat
    20701 ggattttgaa aaaattgaac aagcttatat ctatttacta gagaatgtcc aagtcatcca
    20761 aagtgatttg gcgaccaact tttatgacgc cttggtggag caaaatagca tctatctgga
    20821 tggtgaaact gagctaaacc aggtcaaaga caacaatcag gcccttaagc gtttagcact
    20881 acgcaaagaa gaatggctca agacctacca gtttctcttg atgaaggctg ggcaaacaga
    20941 acccttgcag gccaatcacc agtttacgcc agatgctatt gccttacttt tggtatttat
    21001 tgtggaagag ttgtttacag aggaggaaat tactatcctc gaaatgggtt ctgggatggg
    21061 aattctaggc gctactttct tgacctcgct tgataaaaag gtggattact tgggaatgga
    21121 agtggatgat ttgctgattg atctggcagc tagcatggca gatgtaattg gtttgcaggc
    21181 tggctttgtc caaggagatg ccgttcgccc acaaatgctc aaagaaagcg atgtggtcat
    21241 cagtgacttg cctgtcggct attatcctga tgatgccgtt gcgtcgcgtc atcaagttgc
    21301 ttctagccaa gaacatactt acgcccatca cttgctcatg gaacaagggt ttaagtacct
    21361 taagtcagat ggatatgcta tttttctagc tccgagtgat ttgttgacca gtcctcaaag
    21421 tgatttgttg aaagtctggt tgaaagagga ggcaagtctg gttgctatga ttagtttacc
    21481 tgaaaatctc tttgctaatg ctaaacaatc taagactatt tttatcctac agaagaaaag
    21541 tgaaatagca gtggagcctt ttgtttatcc acttgctagc ttgcaagatg caagtgtttt
    21601 aatgaaattt aaagaaaatt ttcaaaaatg gactcaaggt actgaaatat aaaatagatt
    21661 ttgttataat agttgaaaac gcttaaaaag gggtatcatg ttatgacaaa aacaattgca
    21721 atcaatgcag gaagttcaag tttgaaatgg caattatact taatgccaga ggaaaaagtg
    21781 ttagccaaag gtttgattga acgtatcggt ttgaaagatt caatttcaac tgtaaaattt
    21841 gacggccgtt ctgaacaaca aattttggat attgaaaatc atacacaagc cgttaaaatt
    21901 ttattggatg acttgattcg tctcgatatt atcaaggctt atgacgagat tacaggtgtt
    21961 ggacatcgtg ttgttgctgg tggagaatat ttcaaagaat caacagttgt tgagggagat
    22021 gttttagaaa aagttgaaga gttgagtttg ttggctcctc tacacaaccc ggccaatgca
    22081 gcaggtgttc gtgccttcaa ggaattgttg ccagacatta ccagtgtagt tgtttttgat
    22141 acttccttcc acacaagtat gccagagaaa gcttatcgct accctctacc aacaaaatat
    22201 tacacagaaa acaaggttcg taaatacggt gctcatggta caagtcacca gtttgtagca
    22261 ggagaagctg caaaactctt gggacgtcca ttagaagact tgaagttaat tacctgtcat
    22321 attggtaacg gaggctcaat tacagctgtg aaagccggca aatctgtaga cacttctatg
    22381 gggttcactc ctcttggtgg tattatgatg ggaacgcgta caggggatat tgatccagct
    22441 atcattcctt atttaatgca atatacagag gattttaaca caccagaaga tatcagtcgt
    22501 gttcttaacc gtgaatcagg tcttttggga gtttctgcta attctagcga tatgcgcgat
    22561 atagaagcag ctgtagcaga agggaatcac gaggctagct tggcttatga aatgtatgtt
    22621 gaccgtatcc aaaaacatat cggtcagtac cttgcagtgc taaatggagc agatgtcatt
    22681 gttttcacag caggtgtcgg tgaaaatgca gagagtttcc gtcgtgatgt aatctcaggg
    22741 atttcgtggt ttggttgtga tgttgatgat gaaaagaatg tctttggcgt tacaggagac
    22801 atctcaacag aggcagctaa aatccgtgtc ttggttattc caacagatga agaattagtc
    22861 attgcccgtg acgttgaacg cttgaaaaaa taagtgaaac taaaaaaata ttcaatacaa
    22921 ggagttggga aagttatttt tccagcttct ttttctgatg aaattgtcca aaaccttgct
    22981 atgattggct tttttgaaaa atatggtata atagtagtaa tttaatagat ggagttgagt
    23041 tttgaagaaa aactttcgtg taaaaagaga gaaagatttt aaggcgattt tcaaggaggg
    23101 gacaagtttt gctaatcgca aatttgtggt ctaccaatta gaaaaccaga aaaaccattt
    23161 tcgagtaggt ctatcagtta gcaaaaaact ggggaatgcc gtcactagaa atcaaattaa
    23221 gcgacggatt cggcatatta tccagaatgc aaaagggagt ctggtagaag atgtcgactt
    23281 tgttgtcatt gctcgaaaag gagtcgaaac cttgggatac gcagagatgg agaaaaatct
    23341 actccatgta ttaaaattat caaagattta ccgggaagga aatgggagtg aaaaagaaac
    23401 taaagttgac tagtttgcta ggactgtctc tgttaatcat gacagcctgt gcgactaatg
    23461 gggtaactag cgatattaca gccgaatcgg ctgatttttg gagtaaattg gtttacttct
    23521 ttgcggaaat cattcgcttt ttatcgtttg atattagtat cggagtgggg attattctct
    23581 ttacggtctt gattcgtaca gtcctcttgc cagtctttca ggtgcaaatg gtggcttcta
    23641 ggaaaatgca ggaagctcag ccacgcatta aggcgcttcg agaacaatat ccaggtcgag
    23701 atatggaaag cagaaccaaa ctagagcagg aaatgcgtaa agtatttaaa gaaatgggtg
    23761 tcagacagtc agactctctt tggccgattt tgattcagat gccggttatt ttggccctgt
    23821 tccaagccct atcaagagtt gactttttaa agacaggtca tttcttatgg attaaccttg
    23881 gtagtgtgga tacaaccctt gttcttccga ttttagcagc agtattcacc tttttaagta
    23941 cttggttgtc caacaaagct ttgtctgagc gaaatggcgc tacgactgcg atgatgtatg
    24001 ggattccagt cttgattttt atctttgcag tttatgcgcc aggtggagtc gccctatact
    24061 ggacagtgtc taatgcttat caagtcttgc aaacctattt cttgaataat ccattcaaga
    24121 ttatcgcaga gcgcgaggcc gtagtacagg cacaaaaaga tttggaaaat agaaaaagaa
    24181 aagccaagaa aaaggctcag aaaacgaaat aaataaggag gaatctggta atggtagtat
    24241 ttacaggttc aactgttgaa gaagcaatcc agaaaggatt gaaagaatta gatattccaa
    24301 gaatgaaggc tcatatcaaa gtcatttcta gggagaaaaa aggctttctt ggtctatttg
    24361 gtaaaaaacc agcccaagtg gatattgaag cgattagtga aacgactgtt gtcaaagcaa
    24421 atcaacaggt agtaaaaggc gttccgaaaa aaatcaatga tttgaacgag cctgtgaaga
    24481 cggttagtga agaaaccgtt gaccttggtc atgtagttga tgctattaaa aaaatagagg
    24541 aagaaggtca aggtatttct gatgaagtca aggctgaaat cttaaaacat gaaagacatg
    24601 ccagcactat cttagaagaa actggtcaca ttgagatttt aaatgaactt caaatcgagg
    24661 aagcgatgag ggaagaagca ggcgctgatg accttgaaac tgagcaagac caagctgaaa
    24721 gtcaagaact agaagacttg ggcttgaaag ttgaaacgaa ctttgatatt gaacaagtag
    24781 ctacggaagt aatggcttat gttcaaacga ttattgatga catggatgtt gaggctacac
    24841 tttcaaatga ttataaccgt cgtagcatca atttgcaaat tgatactaac gaaccaggtc
    24901 gtattatcgg ctaccatggt aaagtcttga aggccttgca actgttggct caaaattatc
    24961 tttacaactg ctattcaaga accttctata tcactattaa tgtcaatgat tatgttgaac
    25021 accgtgcaga agtcttgcag acctatgcgc aaaaattggc gattcgtgtt ttagaagaag
    25081 gacgtagtca tcaaacagat ccaatgtcga atagtgaacg caagattatc caccgtatta
    25141 tttcacgtat ggatggcgtg accagttact ctgaaggtga tgagccaaat cgctatgtcg
    25201 ttgtagatac agaataagta aaatcagggt tgacctgatt ttttgctagc tagaggaggt
    25261 taaactgatg ttgaataaga taagagacta tttagacttt gctggtttgc agtaccgtaa
    25321 tcctgataaa gcgggagcag agcgagagaa gatgctggca ttccgccaca aaggacaaga
    25381 ggcccgaaag gcttttacag aactggccaa agcctttcaa gcaagccatc cagaatggca
    25441 actccaacag actagtcagt ggatgaatca ggcccagcgt ttgagaccac atttttgggt
    25501 ttatctacag agagacggac aagtgacaga acctatgatg gccttacgtt tgtatgggac
    25561 atctactgac tttggaattt ctttggaagt cagtttcatc gaacgtaaga aggatgagca
    25621 aacactgggc aagcaggcca aagttttaga cattccaacc gttaaaggga tttattatct
    25681 aacctactct aatggtcaaa gtcaacggtg ggaggcgaat gaagaaaagc gtcgtacttt
    25741 acgcgagaag gtgagaagtc aagaagttcg aaaagtttta gtgaaggtag atgttcctat
    25801 gacagaaaat tcgtctgaag aagaaatcgt agaaggctta ttgaagtctt attctaaaat
    25861 tcttccctat tatctagcta cgagaaaata agataatttg taaaacatca taaatcatac
    25921 agtccaagag tgaacagtcc gctgtgtaat tcttggtctt tttgtttgcg ctttcgcatt
    25981 atataataaa cttacaaaaa caattcaaaa ggagaacaat tatggaagtc gtttcaagtg
    26041 ttctaaattg gttttctagc aatattttgc agaatcccgc atttttcgta ggtttattgg
    26101 tgttgatagg atatgcactt ttgaaaaaac ctgcccatga cgttttttca gggtttgtta
    26161 aagcaacagt agggtatatg ttgcttaacg tgggtgctgg tggtttggtt acaacctttc
    26221 gtccaatctt agcagctctt aactacaaat tccaaattgg tgcagcggtt atcgaccctt
    26281 actttggact tgctgcagca aacaacaaaa ttgcagcaga gtttccagat tttgttggaa
    26341 ctgcaactac agctctattg attggttttg gaataaatat cttgctcgta gctcttcgaa
    26401 agattacgaa ggtaagaacc ctctttatta ctggtcacat catggtacaa caagctgcaa
    26461 cagtatctct tatggttcta ttcttagtac cacaattgcg caatgcttac ggtacagcag
    26521 cgattggtat catctgtgga ctttactggg cagttagttc aaatatgact gttgaggcaa
    26581 ctcaacgctt gactggtggt ggcggatttg cgattggtca ccaacagcaa tttgcaatct
    26641 ggtttgtaga taaagtagca ggacgctttg gtaagaaaga agaaagttta gacaatctta
    26701 aattacctaa gttcctctca atcttccacg atacagttgt tgcatctgct accttgatgc
    26761 tcgtattctt cggagccatt cttttaatct tgggtccaga cattatgtct aataaagaag
    26821 tcatcacttc aggaactcta ttcaatcctg ctaaacaaga tttctttatg tacattatcc
    26881 aaacagcctt taccttctca gtttacttgt tcgttttgat gcaaggtgtc cgaatgttcg
    26941 tatctgagtt gacaaacgcc ttccaaggta tttcaaacaa attgttgcca ggttcattcc
    27001 cagcggttga cgttgcagct tcttatggat ttggttctcc aaatgctgtc ttgtcaggat
    27061 ttacctttgg tttgattggt caattgatta caattgtctt gctcatcgtc tttaaaaatc
    27121 cgattcttat tattacagga tttgtaccag tgttctttga caatgcagcc attgcggtct
    27181 acgctgataa acgcggcgga tggaaagcgg ctgttatcct ttcctttata tcaggtgtcc
    27241 ttcaagttgc tctaggagct ctttgtgtgg cccttctcga tttggcatct tatggtggct
    27301 accatggaaa tatcgacttt gaattcccat ggcttggatt tggatatatc ttcaaatacc
    27361 ttggtattgt tggttatgta cttgtgtgtc tcttcttgct tgttattcct caacttcaat
    27421 ttgccaaagc aaaagataaa gagaaatatt acaacggtga agttcaagaa gaagcttagt
    27481 atctagaaaa ggagaaataa aatggttaaa gtattatcag cgtgcggaaa tggaatgggt
    27541 tcatcaatgg ttatcaagat gaaggttgaa aatgctctcc gtaagcttaa tcaaacagat
    27601 tttacagtca attcatgcag tgtcggtgaa gctaaaggtt tagcagtagg atatgacatc
    27661 gtaatcgctt ctcttcattt gattcaagaa ttggaagggc gaactaatgg gaagttaatt
    27721 gggcttgata acttgatgga tgataaagaa atcaccgaaa aactcagtca agcactacag
    27781 taaaaggttg gagggggctg gacagaaact gagagttatc gtttctgtcc ttctccctct
    27841 ttaaataaag gaggcagata tgaatttaaa acaagcttta attgacaatg actcgatccg
    27901 actaggttta gaggctaaca attggaaaga agcagtcaag gtagcagtag atcccttaat
    27961 tgaaagtggg gcaattttgc cagagtatta cgatgctatc attgaatcga ctgaagagta
    28021 tgggccttac tatatcttga tgccaggtat ggctatgccc cacgctagac ctgaagctgg
    28081 tgtgcaaagt gatgcctttt cattgattac cttacaaaat cctgttgtat tttcagatgg
    28141 gaaagaggta tctgttttgt tggcactagc agcaacaagt tcaaaaattc acacaagtgt
    28201 agccattcca caaattattg ccctgtttga attagaagat tctattgcac gtttacaggc
    28261 ttgccagact aaagaagatg tcttggctat gattgaagaa tctaaggata gcccttatct
    28321 cgaaggattg gatttggaaa gttagaaaga ggaataaaga aatgacaaaa agaataccta
    28381 atttacaagt tgcattagac cattcagact tgcaaggagc gattaaagca gctgtttctg
    28441 ttggtcagga agtagatatt atcgaagctg gaactgtttg cttgcttcaa gttggaagtg
    28501 aactggctga agtcttgcgt agccttttcc cagataagat tattgtggca gacacaaaat
    28561 gtgctgatgc tggtggaaca gttgctaaaa ataatgcggt tcgtggagca gactggatga
    28621 cttgtatctg ttgtgcaacc atctctacta tggaagcagc tctaaaggct atcaagactg
    28681 aacgaggaga acgaggcgaa atccagatcg agctttatgg cgattggact tttgaacaag
    28741 ctcagctttg gctagatgca ggtatctcac aagctattta tcaccaatct cgtgatgctc
    28801 ttcttgctgg tgaaacttgg ggtgaaaaag accttaataa ggttaaaaaa ctcattgata
    28861 tgggcttccg tgtatctgta acaggtggtc tagatgtaga tactctcaaa ctctttgaag
    28921 gtgttgatat ctttaccttt atcgcaggtc gtggaattac agaggctgcg gatccagcag
    28981 gagcagcgcg tgccttcaag gatgaaatca aacgaatttg ggggtaaatc atggtacgtc
    29041 caattggaat ttatgaaaag gcaaccccaa cacactttac ttggctagaa cgtttaaatt
    29101 ttgccaagga gttaggcttt gattttatcg agatgtctat tgacgaacgt gacgagcgtt
    29161 tagcaagact tgactggagt aaggaagaac gcttggaagt tgtcaaagaa atctatgaaa
    29221 ctggtgttcg tattccttct atctgttttt caggccatcg tcgctaccca ttgggatcaa
    29281 aagattcagt tctagaggaa aaatctctag aactcatgaa aaaatgtatc gaattagctc
    29341 aagacttggg agttcgtacg attcaattag ctggttacga tgtttactat gaggaaaagt
    29401 caccccagac acgccaacgt tttatcaaaa atttgagaaa agcctgtgac tgggctgaag
    29461 aagctcaggt ggtacttgct attgaaatta tggatgatcc tttcatcagt agcatcgaaa
    29521 aatatttggc tatagaaaaa gagattgact ctcccttcct ctttgtatat ccagatattg
    29581 gtaatgtgtc tgcatggcat aatgatatct atagtgagtt ttatcttggt catcatgcca
    29641 tcgcagctct ccatctcaag gatacttatg cagtgacaga aagttcaaag ggccagttcc
    29701 gagatgtacc tttcgggcaa ggttgtgtca aatgggaaga agctttcgat attttaaagg
    29761 aaaccaatta taatggacct ttcctaatcg aaatgtggtc tgaaaattgt gaaacagtag
    29821 aagaaacacg cgcagccatt caagaggcgc aagcttttct ctatccactc attaagaaag
    29881 caggtttgat gtaagatgaa tcaagtaatc aatgctatgc gtaaacgagt ctgtgatgcc
    29941 aatcaatcat tgccaaaaca tggacttgtc aaatttacct gggggaatgt atctgaagtc
    30001 aatcgcgaac tcggtgtcat tgttatcaaa ccatcaggcg tggattatga cgaattgaca
    30061 cctgaaaaca tggtagtgac tgatctagat ggtaagatcc tagaagggga tttaagacca
    30121 tcttccgacc tcccaactca tgtgcaatta tataaggctt ggtcagaaat tggtagtgtg
    30181 gttcacaccc attcgacaga agctgttgct tgggctcagg caggtcgtga tattcctttc
    30241 tacggaacaa cccatgcaga ttatttctac ggttcaattc cttgcgcccg tagtttgacc
    30301 aaggacgaag tagaagtggc ctatgaaaaa gatactggcc tggttatcgt agaagagttt
    30361 gaacatcgcg gacttaaccc ggttgaagta ccaggaattg ttgtacgcaa tcacggtcca
    30421 ttcacctggg gcaaaaatcc agagaatgct gtttatcact ctgtcgtact agaggaagta
    30481 tcaaagatga atcgctttac agaacaaatc aatccaagag ttggacctgc tccccagtac
    30541 atactagaaa aacactacca acgtaaacat ggaccaaatg cttattatgg tcaaaagtaa
    30601 gaacgatgaa ggaggagaaa aagataaatt tagctcctct ttttacattt gatttttatt
    30661 gagagtaaag ttggagttga agtaatttta aaagattttt tagaaatagc gcttgatata
    30721 tatatggtaa aataaaaaga attgctgtga tatcaataga tttgggggat tttttaatat
    30781 ggtactggat aaggcaagtt gtgatttgct tcaatatttg atggatcaag aaacgtccaa
    30841 aacgattatg gcgatttcga aagatttgaa agagtcaaga aggaaaattt attatcacat
    30901 tgacaaaatc aatgctgctc tgggtgacga ggcgcttcac atcattagta ttccacgaat
    30961 tggtattcac ttaacggaag agcagagaga tgcttgttgt aaactattat cggaagtaga
    31021 ttcgtacgat tatatcatga gtgcgcatga acgtatgatg ataatgttac tatggatagg
    31081 tatttctaaa gaacgtatta cgattgaaaa attgatagag ttaacagagg tatctaggaa
    31141 tactgttctc aatgatttga atagtattcg ttatcaacta actttggaac aatatcaggt
    31201 gatcttgcaa gtgagcaagt cacagggata ccaccttcat gcccaccctc ttaataaaat
    31261 tcagtatctt caatcgcttc tatatcatat ttttatggaa gaaaatgcca cttttgtatc
    31321 tattttagaa gataagatga aagagaggtt agatgatgag tgtttgcttt ctgttgaaat
    31381 gaaccaattt tttaaggaac aggttccttt agttgaacaa gatttaggga agaaaataaa
    31441 tcatcatgaa ataactttta tgttgcaggt tctaccttat ttgctgttaa gctgtcataa
    31501 tgttgaacag tatcaagaaa gacatcagga tatagagaaa gaattttctt tgataagaaa
    31561 aagaatagag tatcaggtgt ctaagaaatt aggagaacgg ttgtttcaaa agtttgaaat
    31621 ttctttgtca ggacttgaag tttctcttgt agctgttctc ctcctctcct atcgtaaaga
    31681 tttggatatt catgcagaaa gtgatgattt tcggcaatta aaacttgctt tagaagaatt
    31741 tatctggtat tttgaatcac aaatccgaat ggagattgag aacaaggatg atttgttacg
    31801 aaatttgatg atccactgta aagccttgtt atttagaaag acttacggta ttttttctaa
    31861 aaatcctcta acaaaacaaa ttcgatccaa gtatggagaa ttatttttag tcactagaaa
    31921 atctgcggaa attttagaag gagcatggtt tattcggcta acagacgatg atattgccta
    31981 tttgacgatt catattggag gatttttaaa gtatacacca tcgtctcaaa aaaatatgaa
    32041 aaaagtttat ctcgtttgtg atgaaggtgt tgcggtttcg agacttttgc tgaaacaatg
    32101 caaactttat tttccaaatg agcaaattga cactgtattt acaacagaac aatttaagag
    32161 tgtggaagat attgcacaag ttgatgtagt gattactact aatgatgatt tggatagcag
    32221 atttccgatt ttaagggtta atcctatcct tgaagcagaa gatattttga aaatgctaga
    32281 ctatcttaaa cacaatatat ttcgtaataa gagcaaaagt ttcagtgaaa atctttctag
    32341 tcttatttcg tcttatattg tagacagcaa gttggctagt aagttccaag aagaggttca
    32401 aacacttata aatcaagaaa tagtagttca agcttttttg gaagatattt gaaggacagt
    32461 ccaatgatga acacaaacct gtgtttttct tggtcttttt tagtgttttg aagggtggta
    32521 tactaatctc aaagataaca attatatcca aaggaggcaa catatgccaa acgtcaaaga
    32581 aattacaaga gagtcatgga ttttagccac tttcccagag tggggaacat ggttgaacga
    32641 agaaatcgaa gaagaagtcg tacctgaagg caactttgcc atgtggtggc taggcaactg
    32701 tggtacttgg attaagacac cagctggtgc taacgttgtc atggaccttt ggtcaaaccg
    32761 tggaaaatca accaaaaaag tgaaagatat ggttcgtggg caccaaatgg caaatatggc
    32821 aggtgttcgt aaattgcaac caaacttgcg tgttcagcca atggttatcg atccatttgc
    32881 tatcaacgaa ctagactatt acttagtttc acacttccac agtgatcata tcgacccata
    32941 cacagctgca gcaattctca acaatcctaa gttagagcat gttaagttta tcggtcctta
    33001 ccactgtgga cgaatctggg aaggatgggg ggttccaaaa gaacgtatca tcgttgttaa
    33061 accaggtgac actatcgaat taaaagatat gaagattcat gcagtagaat catttgaccg
    33121 tacttgcttg gtaactctcc cagtgaacgg tgctgatgag acaggcggtg aacttgctgg
    33181 cttggctgtt acagatgaag aaatggctca aaaggctgtt aactatatct ttgaaacacc
    33241 aggtggaacc atctatcatg gtgcagattc tcacttctca aactattttg caaaacatgg
    33301 taaagacttt aaaattgatg ttgctttgaa taactatggt gaaaatccgg taggtatcca
    33361 agacaaaatg acatctatcg accttcttcg tatggcagaa aatctgcgta ccaaagtcat
    33421 tatcccagtt cactatgata tctggtctaa cttcatggct tctactaatg agattctaga
    33481 actttggaaa atgcgaaaag atcgcttgca atacgatttc catccattta tctgggaagt
    33541 tggcggtaag tacacttatc ctcaagatca acacttagta gaataccatc atccacgtgg
    33601 ttttgatgat tgttttgaac aagactctaa cattcaattt aaagctttgc tataatataa
    33661 aaatattctt tggaggttat ccgtatgttg catgatacgg ataattttca tataatagta
    33721 aaatagaatg tgtgattcaa taatcacctc aaatagaaag gaaattctat gtcaaatcta
    33781 tctgttaatg caattcgttt tctaggtatt gacgccatta ataaagccaa ctcaggtcat
    33841 ccaggtgtgg ttatgggagc ggctccgatg gcttacagcc tctttacaaa acaacttcat
    33901 atcaatccag ctcaaccaaa ctggattaac cgcgaccgct ttattctttc agcaggtcat
    33961 ggttcaatgc tcctttatgc tcttcttcac ctttctggtt ttgaagatgt cagcatggat
    34021 gagattaaga gtttccgtca atggggttca aaaacaccag gtcacccaga atttggtcat
    34081 acggcaggga ttgatgctac gacaggtcct ctagggcaag ggatttcaac tgctactggt
    34141 tttgcccaag cagaacgttt cttggcagcc aaatataacc gtgaaggcta caatatcttt
    34201 gaccactata cttacgttat ctgtggagac ggagacttga tggaaggtgt ctcaagcgag
    34261 gcagcttcat acgcaggttt gcaaaaactt gataagttgg ttgttcttta tgattcaaat
    34321 gatatcaact tggatggtga gacaaaggat tcctttacag aaagtgttcg tgaccgttac
    34381 aatgcctacg gttggcatac tgccttggtt gaaaatggaa cagacttgga agccatccat
    34441 gctgctatcg aaacagcaaa agcttcaggc aagccatctt tgattgaagt gaagacggtt
    34501 attggatacg gttctccaaa caaacaagga actaatgctg tacacggcgc ccctcttgga
    34561 gcagatgaaa ctgcatcaac tcgtcaagcc ctcggttggg actacgaacc atttgaaatt
    34621 ccagaacaag tatatgctga tttcaaagaa catgttgcag accgtggcgc atcagcttat
    34681 caagcttgga ctaaattagt tgcagattat aaagaagctc atccagaact ggctgcagaa
    34741 gtagaagcca tcatcgacgg acgtgatcca gtcgaagtga ctccagcaga cttcccagct
    34801 ttagaaaatg gtttttctca agcaactcgt aattcaagtc aagatgcctt gaatgttgtg
    34861 gcagctaaat taccaacctt cctaggtgga tcagctgacc ttgctcactc aaacatgact
    34921 tatatcaaga cggatggact tcaagacgat accaatcgct tgaatcgtaa cattcagttt
    34981 ggtgttcgtg aatttgcaat gggaacaatc ttgaacggga tggcccttca tggtggactt
    35041 cgtgtatacg gtggaacttt cttcgtcttc tctgactatg tgaaggcagc tgtccgcttg
    35101 tcagccttac aaggacttcc tgtgacttat gtctttaccc atgattcaat cgcagttggg
    35161 gaagatggtc cgactcacga accggttgag catttagcag gtcttcgtgc tatgccaaat
    35221 ctaaatgttt tccgtccagc agatgcgcgt gaaacgcaag cagcttggta ccttgcagtg
    35281 acaagtgaga aaacaccaac tgcccttgtc ttgacacgtc aaaacttgac tgttgaagat
    35341 ggaacagact tcgacaaggt tgctaaaggt gcttatgttg tatatgaaaa tgcagccgac
    35401 tttgatacca tcttgattgc gacaggttca gaggttaatc ttgctgtctc agctgctaaa
    35461 gaattggcta gtcaaggcga aaaaatccgc gtagtcagca tgccatctac agatgtcttt
    35521 gataaacaag atgcagctta caaggaagag attcttccaa atgcagtccg ccgtcgtgtt
    35581 gcagtcgaaa tgggtgcaag tcaaaactgg tacaaatatg ttggtctcga tggtgccgtt
    35641 ctaggtatta ataccttcgg agcctctgcc ccagcaccaa aagtattggc agaatatggc
    35701 tttactgtag aaaatcttgt aaaaattgtt cgaaacttga aataatccta aaaatcaggg
    35761 cgtaagctct ggtttttctt accagaaaag taaggtacaa tcttgtaaaa gtagctgaaa
    35821 tttgatatag tagtcctatg taaaagacaa aggagaaaac aatgaatcca aatattactt
    35881 ttttaatcat gcttgtaggt atgatggcct tgatgttctt tatgcaacgt tctcaaaaga
    35941 aacaagctca aaagcgtatg gaaagcttaa ataaactaca aaaaggctat gaagtgatta
    36001 caatcggtgg actttatgga acagtcgatg aagtagatac ggagaaggga acaatagttc
    36061 ttgacgtaga tggagtttac ttgacttttg aactagctgc tatcaagaca gtattaccgc
    36121 tgaaagaaac agcttcacta gaaggcgcaa ttgaaaaata agacgggatc acgaactccc
    36181 gtttttctat aaaagaaagg aaatgggatg aaaaaattag tctttgtctg tctgggaaat
    36241 atttgccgta gccctatggc cgagtttgtt atgaaatcaa tgacagataa ctacgaaatc
    36301 caaagtcgag caacttcctc ttgggaacat ggcaatccga ttcataaggg gactcaggga
    36361 atttttcaag agtatgagat tccttatgac aagaacaaga catcgcttca gattagtaag
    36421 gaagattttg aagcctttga ttatattatc ggaatggacg cttcaaatat ttctgactta
    36481 cgtcagatgt gtccagtaga ctgtcaagat aagatttact cattttcatc tgaaagtgtt
    36541 ccagaccctt ggtatacagg agattttgaa gaaacctatc gacgtgttca agagggctgt
    36601 caagcttggt tagaacgttt agaaaaggag agttaaggtg gaaaatctta cgaattttta
    36661 cgaaaagtat cgtgtctatc tgactcgtcc acgattagag cttttggcag tagttaccat
    36721 tgttttctgt gctgtactcg tcttttttct aaatattcca ggaaaaggtg tcttaaaact
    36781 cgataatgga acgattgttt atgatggcag tcttgtccgt ggtaaaatga atggtcaagg
    36841 taccattacc ttccaaaatg gagaccaata tacaggtggc ttcaacaatg gagccttcaa
    36901 cggaaaaggt acctttcaat ctaaagaagg ctggacctac gaaggtgatt ttgtaaatgg
    36961 tcaggctgaa ggaaaaggga aactaacaac agaacaagaa gtcgtttatg aaggaacttt
    37021 taaacaaggc gtttttcaac aaaaataaag cctccttatc aaaggaggta ttattagaat
    37081 cgcaaggtaa gcgcttacct gcaaatccct ttctttccaa atccctcttc caaccaagtt
    37141 tgtgaaataa aaaatatttg aaataaattt cacaaacttc aaagataaaa cctgataaga
    37201 aaagaaaatg agaaaagttt cacaagagtt taaaaattct ttgtgaaatg tttatgaaac
    37261 tgtttacaaa gttgaaataa tgcgttataa taaacttgtg aaaaaattaa caaaggatat
    37321 cgttccttga aagctatgga ggaaaatatg gctgataaaa aaactgtgac accagaggaa
    37381 aagaaactcg ttgctgaaaa acacgtagat gagttggttc aaaaagctct agttgccctt
    37441 gaagaaatgc gtaaattgga tcaagaacaa gttgactaca tcgttgccaa agcatcagta
    37501 gcagctttgg atgcccacgg agaattggct ttacatgcct ttgaagaaac aggacgtggt
    37561 gtatttgaag ataaagcaac taagaacttg tttgcttgtg aacacgtggt aaacaacatg
    37621 cgtcacacta aaacagttgg cgttatcgaa gaagatgatg taacaggatt gaccctgatt
    37681 gctgaaccag ttggtgttgt ttgtggtatc actccaacaa caaacccaac atcaacagcg
    37741 atcttcaaat cattgatttc attgaagaca cgtaatccaa tcgtctttgc cttccatcca
    37801 tcagctcaag aatcatcagt tcatgcagct cgtatcgtcc gcgatgcagc tatcgcagct
    37861 ggtgctcctg aaaactgtgt acagtggatc actgaaccat ctatggaagc aactagtgcc
    37921 cttatgaacc acgaaggtat tgcaacaatc cttgcaacag gtggtaatgc catggttaaa
    37981 gccgcatact catgtggtaa accagctctt ggggtaggtg ccggaaacgt tccagcttat
    38041 gttgaaaaat cagcaaacat tcgtcaagca gcacacgata tcgtcatgtc taaatcattt
    38101 gataacggta tggtctgtgc atctgaacaa gcagttatca ttgataaaga aatttacgat
    38161 gaatttgtag cagagttcaa atcttaccac acttactttg taaacaaaaa agaaaaagct
    38221 cttcttgaag agttctgctt cggcgtcaaa gcaaacagca aaaactgtgc tggtgcaaaa
    38281 ttgaacgctg acatcgttgg taaaccagca acttggattg cagaacaagc aggatttaca
    38341 gttccagaag gaacaaacat tcttgctgca gaatgtaaag aagttggcga aaatgagcca
    38401 ttgactcgtg aaaaattgtc accagttatt gcagttttga aatctgaaag ccgtgaagat
    38461 ggtattacta aggctcgtca aatggttgaa tttaacggtc ttggacactc agcagctatc
    38521 cacacagctg acgaagaatt gactaaagaa tttggtaaag ctgttaaagc tattcgtgtt
    38581 atctgtaact caccttctac ttttggtggt atcggggacg tttacaatgc cttcttgcca
    38641 tcattgacac ttggatgtgg ttcttacgga cgcaactcag ttggggataa cgttagtgcc
    38701 attaacctct tgaatatcaa aaaagtcgga agacggagaa ataacatgca atggatgaaa
    38761 cttccttcaa aaacatactt tgaacgtgat tcaattcaat accttcaaaa atgtcgtgac
    38821 gttgaacgtg tcatgatcgt tactgaccat gccatggtag agcttggttt ccttgatcgt
    38881 atcatcgaac aactggacct tcgtcgcaat aaggttgttt accaaatctt tgcggatgta
    38941 gaaccggatc cagatatcac aactgtaaac cgtggtactg agattatgcg tgccttcaga
    39001 ccagatacca tcatcgcact cggtggtggg tctccaatgg atgctgccaa agtaatgtgg
    39061 ctcttctacg agcaaccaga agtggacttc cgtgaccttg tccaaaaatt catggatatc
    39121 cgtaaacgtg ccttcaagtt cccattgctt ggtaagaaga ctaaattcat cgcgattcca
    39181 actacatctg gtacaggatc tgaagtaaca ccatttgccg ttatctctga taaagcaaac
    39241 aaccgtaaat acccaatcgc tgactactca ttgacaccaa ctgtggcaat tgtagaccct
    39301 gctttggtat tgacagttcc aggatttgtt gctgctgata ctggtatgga cgtattgact
    39361 cacgcgacag aagcatacgt atcacaaatg gctagtgact acactgatgg tttagcactt
    39421 caagctatta aattggtctt tgaaaatctc gaaagctcag ttaagaatgc agacttccac
    39481 tcacgtgaga aaatgcataa cgcttcaaca atcgctggta tggcctttgc caatgccttc
    39541 ctaggtattt ctcactcaat ggcccataag attggtgcgc aattccacac aatacacggt
    39601 cgtacaaatg ctatcttgct tccatacgtt atccgttaca acggtacacg tccagctaag
    39661 acagcaacat ggcctaagta caactactac cgtgcagatg aaaaatacca agatatcgca
    39721 cgcatgcttg gacttccagc ttctactcca gaagaagggg ttgaatctta cgcaaaagct
    39781 gtctacgaac tcggtgaacg tattgggatc caaatgaatt ttagagacca aggaattgac
    39841 gaaaaagaat ggaaagaaca ttctcgtgaa ttagccttcc tggcttatga agaccaatgt
    39901 tcaccagcta acccacgtct tccaatggta gaccatatgc aagaaatcat cgaagatgca
    39961 tactatggct acaaagaaag accaggacgc cgtaaataat tgtttatcag tctagaagca
    40021 agacaaaaac tcaatttgag ggaaagatcc agtaattttt ctatgataaa aggcatccta
    40081 tcaaggtttt tgaacacctg ataggatgcc tttttatgat attgaggcct ttttgccctt
    40141 tttgaaaaac tagaatagaa acaaaatata taatagattg aaactggaat agtacacttc
    40201 tgcttctaaa acattgttag aattcgattt gactgtcctg atcgatttgt cctattccta
    40261 tttcatttta ttatataaaa aatata
//
LOCUS       DAVLXF010000006        35014 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLXF010000006 DAVLXF010000000
VERSION     DAVLXF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.006"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            16..210
                     /locus_tag="VYO12_000311"
     CDS             16..210
                     /locus_tag="VYO12_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1586975.1"
                     /translation="MEAEAIRRNCERGAIFSGKIKYHEDSQFKGDHYVECYAVLDNTV
                     IARDRITVPIDPLCGKDFIE"
     gene            455..1789
                     /gene="trmFO"
                     /locus_tag="VYO12_000312"
     CDS             455..1789
                     /gene="trmFO"
                     /locus_tag="VYO12_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1586976.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1873..2610
                     /locus_tag="VYO12_000313"
     CDS             1873..2610
                     /locus_tag="VYO12_000313"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1586977.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2619..3176
                     /gene="frr"
                     /locus_tag="VYO12_000314"
     CDS             2619..3176
                     /gene="frr"
                     /locus_tag="VYO12_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1586978.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3236..4090
                     /gene="cvfB"
                     /locus_tag="VYO12_000315"
     CDS             3236..4090
                     /gene="cvfB"
                     /locus_tag="VYO12_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1586979.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4099..4314
                     /locus_tag="VYO12_000316"
     CDS             4099..4314
                     /locus_tag="VYO12_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1586980.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDAIWQEYLEH"
     gene            4400..5368
                     /locus_tag="VYO12_000317"
     CDS             4400..5368
                     /locus_tag="VYO12_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1586981.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5346..5525)
                     /locus_tag="VYO12_000318"
     CDS             complement(5346..5525)
                     /locus_tag="VYO12_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586982.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            5561..6061
                     /locus_tag="VYO12_000319"
     CDS             5561..6061
                     /locus_tag="VYO12_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1586983.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGFFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            6064..6323
                     /locus_tag="VYO12_000320"
                     /pseudo
     CDS             6064..6323
                     /locus_tag="VYO12_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6690..7016)
                     /locus_tag="VYO12_000321"
     CDS             complement(6690..7016)
                     /locus_tag="VYO12_000321"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586984.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(6962..7375)
                     /locus_tag="VYO12_000322"
     CDS             complement(6962..7375)
                     /locus_tag="VYO12_000322"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV1586985.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(7376..7801)
                     /locus_tag="VYO12_000323"
     CDS             complement(7376..7801)
                     /locus_tag="VYO12_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1586986.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDVLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            7978..8547
                     /locus_tag="VYO12_000324"
     CDS             7978..8547
                     /locus_tag="VYO12_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1586987.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLVNPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8615..9265
                     /locus_tag="VYO12_000325"
     CDS             8615..9265
                     /locus_tag="VYO12_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV1586988.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     VEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            9249..11489
                     /locus_tag="VYO12_000326"
     CDS             9249..11489
                     /locus_tag="VYO12_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1586989.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKAAQHGSKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGWNSW
                     KIESVR"
     gene            11631..11855
                     /locus_tag="VYO12_000327"
     CDS             11631..11855
                     /locus_tag="VYO12_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586990.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYYSYSEYPVGSGWNYARYEVINYYTGGY"
     gene            11887..12474
                     /locus_tag="VYO12_000328"
     CDS             11887..12474
                     /locus_tag="VYO12_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1586991.1"
                     /translation="MLRLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            12478..13659
                     /locus_tag="VYO12_000329"
     CDS             12478..13659
                     /locus_tag="VYO12_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1586992.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDISSEEYQFAKNNLDVQKNLLKRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQAMYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    13819..13950
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            13966..14496
                     /gene="infC"
                     /locus_tag="VYO12_000330"
     CDS             13966..14496
                     /gene="infC"
                     /locus_tag="VYO12_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1586993.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14529..14729
                     /gene="rpmI"
                     /locus_tag="VYO12_000331"
     CDS             14529..14729
                     /gene="rpmI"
                     /locus_tag="VYO12_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1586994.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14781..15140
                     /gene="rplT"
                     /locus_tag="VYO12_000332"
     CDS             14781..15140
                     /gene="rplT"
                     /locus_tag="VYO12_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1586995.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15198..15578
                     /locus_tag="VYO12_000333"
     CDS             15198..15578
                     /locus_tag="VYO12_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1586996.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            15828..16628
                     /locus_tag="VYO12_000334"
     CDS             15828..16628
                     /locus_tag="VYO12_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1586997.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            16591..17577
                     /locus_tag="VYO12_000335"
     CDS             16591..17577
                     /locus_tag="VYO12_000335"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1586998.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGAAAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17677..19785
                     /gene="lytB"
                     /locus_tag="VYO12_000336"
     CDS             17677..19785
                     /gene="lytB"
                     /locus_tag="VYO12_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV1586999.1"
                     /translation="MKKVRFIFLALLFFLASPEGAMASDGTWQGKQYLKEDGSQAANE
                     WVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGYMAKSEWVEDKGAFYYLDQD
                     GKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKADGQHAEKEWLQIKGKDYYF
                     KSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFYIKENGNYADKEWIFENGHY
                     YYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDSHSQAWYYFKSGGYMAANEW
                     IWDKESWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDG
                     KIAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQ
                     AWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNKNAAYYQVVPVTANVYDSDGEKL
                     SYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRF
                     YHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEEL
                     DKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFF
                     GITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPY
                     WGEKIASVMMKINEKLGGKD"
     gene            complement(19846..21501)
                     /gene="pavA"
                     /locus_tag="VYO12_000337"
     CDS             complement(19846..21501)
                     /gene="pavA"
                     /locus_tag="VYO12_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1587000.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21606..22103
                     /gene="ybeY"
                     /locus_tag="VYO12_000338"
     CDS             21606..22103
                     /gene="ybeY"
                     /locus_tag="VYO12_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1587001.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22084..22479
                     /locus_tag="VYO12_000339"
     CDS             22084..22479
                     /locus_tag="VYO12_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1587002.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22496..23395
                     /gene="era"
                     /locus_tag="VYO12_000340"
     CDS             22496..23395
                     /gene="era"
                     /locus_tag="VYO12_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1587003.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKIPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23444..24268
                     /gene="mutM"
                     /locus_tag="VYO12_000341"
     CDS             23444..24268
                     /gene="mutM"
                     /locus_tag="VYO12_000341"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1587004.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24268..24873
                     /locus_tag="VYO12_000342"
     CDS             24268..24873
                     /locus_tag="VYO12_000342"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1587005.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDTQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24860..26059
                     /locus_tag="VYO12_000343"
     CDS             24860..26059
                     /locus_tag="VYO12_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV1587006.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26059..26208
                     /gene="rpmG"
                     /locus_tag="VYO12_000344"
     CDS             26059..26208
                     /gene="rpmG"
                     /locus_tag="VYO12_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1587007.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26248..26481
                     /gene="secG"
                     /locus_tag="VYO12_000345"
     CDS             26248..26481
                     /gene="secG"
                     /locus_tag="VYO12_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1587008.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26583..28937
                     /gene="rnr"
                     /locus_tag="VYO12_000346"
     CDS             26583..28937
                     /gene="rnr"
                     /locus_tag="VYO12_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1587009.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     AGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYLEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRDSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28900..29367
                     /gene="smpB"
                     /locus_tag="VYO12_000347"
     CDS             28900..29367
                     /gene="smpB"
                     /locus_tag="VYO12_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1587010.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKRQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29383..30243
                     /gene="tehB"
                     /locus_tag="VYO12_000348"
     CDS             29383..30243
                     /gene="tehB"
                     /locus_tag="VYO12_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1587011.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMETVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30316..31269
                     /gene="coiA"
                     /locus_tag="VYO12_000349"
     CDS             30316..31269
                     /gene="coiA"
                     /locus_tag="VYO12_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1587012.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIQQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31288..33090
                     /gene="pepF"
                     /locus_tag="VYO12_000350"
     CDS             31288..33090
                     /gene="pepF"
                     /locus_tag="VYO12_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1587013.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33092..33805
                     /locus_tag="VYO12_000351"
     CDS             33092..33805
                     /locus_tag="VYO12_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1587014.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEEFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33872..34813
                     /gene="prsA"
                     /locus_tag="VYO12_000352"
     CDS             33872..34813
                     /gene="prsA"
                     /locus_tag="VYO12_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1587015.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34930..>35014)
                     /locus_tag="VYO12_000353"
     CDS             complement(34930..>35014)
                     /locus_tag="VYO12_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1587016.1"
                     /translation="IDYYNNKRIKVKLKGLSPVQYRTKSFR"
BASE COUNT        10538 a         6510 c         7920 g        10046 t
ORIGIN      
        1 tggaaagtaa gaaatattga agctgaggct attagaagaa attgtgagcg tggtgctatt
       61 ttttcaggta aaataaaata tcacgaagat tcacagttta aaggagatca ctatgttgaa
      121 tgttatgctg ttttagataa tacggttata gcaagagata gaataacagt ccctatcgat
      181 ccgttatgtg gaaaagattt tatagagtag catataattg attcttaact ggaatactca
      241 ctatctcttt acatcaagaa aatgactaaa cagggaagtt tgccttcttc cctttttttg
      301 ttatactagt agaagaaaaa atagaaagat ttgtgggagt gaaaaagtcc tgtggacttt
      361 ttcagcctga gccaagaaac tcgaaagctt gtaagtctgc ttggcttttt caatgtgaat
      421 cttaacttca cactcccaaa gaggtattag tgtcgtgtct caatcttata tcaatgttat
      481 cggtgctggt ttggcaggtt ctgaagcagc ttaccaaatc gcagagcgtg gtattccagt
      541 taaactatat gaaatgcgtg gtgtcaagtc tacaccccag cataaaacag acaattttgc
      601 tgagttggtt tgttccaatt ctttgcgtgg ggatgctttg acaaatgcag ttggtcttct
      661 caaggaagaa atgcgtcgct tgggttctgt tatcttggaa tctgctgagg ctacacgtgt
      721 tcctgccggt ggagcccttg cggtggatcg tgatggtttc tctcaaatgg tgaccgaaaa
      781 agtggtcaac caccccttga ttgaagtggt gcgtgatgaa attacagaat tgccaacaga
      841 tgttattaca gttgtggcga ctggtccctt gactagcgat gccctggctg aaaagattca
      901 tgcccttaat aatggcgatg gcttctattt ctacgatgca gcagcgccta ttatcgatgt
      961 caacactatc gatatgagta aggtctatct caaatctcgt tatgataagg gagaagcagc
     1021 ctatctcaat gctccaatga ccaagcaaga gtttatggat ttccatgaag ccttggtcaa
     1081 tgcggaagaa gcaccgctca attcttttga aaaagaaaag tactttgaag gatgtatgcc
     1141 aatcgaagtt atggccaaac gtggcattaa aactatgctt tatggcccta tgaagccagt
     1201 cggtcttgag tatccggacg actacacagg acctcgtgat ggagaattta aaacacctta
     1261 tgcggttgtg caacttcgtc aggataatgc agctggtagc ctatacaata ttgttggttt
     1321 ccagacccac ctcaaatggg gagaacaaaa gcgtgtcttc caaatgattc cgggtcttga
     1381 aaatgcggag tttgtccgtt atggtgtcat gcatcgcaat tcttacatgg attcaccaaa
     1441 tcttcttgag cagacttacc gttccaagaa acaaccaaat ctcttctttg ctggtcagat
     1501 gacgggggtg gaaggctatg ttgagtcggc ggcttcgggc ttagttgcgg gaattaacgc
     1561 agctcgtctc ttcaaggaag aaagcgaggt tattttcccc gagacgacag cgattggaag
     1621 cttagctcat tacattaccc atgccgacag caaacatttc caaccaatga atgtcaattt
     1681 tgggatcatc aaggagttgg aaggcgagcg tatccgtgat aagaaggctc gttatgaaaa
     1741 aattgcagag cgtgcccttg ccgacttaga ggaatttttg actgtctaat ttttttgaaa
     1801 gaattgttca tgatactata aaaatcttag aaattgtgat aaaataggta ggatgaaaga
     1861 aggagagtga aaatggcgaa tcccaagtat aaacgtattt taatcaagtt atcaggtgaa
     1921 gcccttgccg gtgaacgtgg cgtagggatt gatatccaaa cagttcaaac aatcgcaaaa
     1981 gagattcaag aagttcatag cttaggtatc gaaattgccc ttgttattgg tggaggaaat
     2041 ctctggcgtg gagaacctgc agcagaagca ggtatggacc gtgttcaggc agattacact
     2101 ggaatgcttg ggactgttat gaatgctctt gtgatggcag attcattgca acaagttggg
     2161 gttgatacgc gtgtacaaac agctattgct atgcaacaag tggcagagcc ttatgtccgt
     2221 ggacgtgccc ttcgtcacct tgaaaaaggc cgtatcgtta tctttggtgc tggaattggt
     2281 tcaccatact tctcgacaga tacaacagcg gcccttcgtg cagctgaaat cgaagcagat
     2341 gccatcctca tggctaaaaa tggcgtcgat ggtgtgtaca atgccgatcc taagaaggac
     2401 aagacagccg ttaagtttga agaattgacc caccgtgatg ttatcaacaa aggtcttcgt
     2461 atcatggact caacagcctc aaccctctca atggacaacg acattgactt ggttgtcttc
     2521 aacatgaacc aatcaggcaa catcaaacgt gtcgtatttg gtgaaaatat cggaacaaca
     2581 gtttcaaata atatcgaaga aaaggaataa gaaagaatat ggctaacgta attattgaaa
     2641 aagctaaaga gagaatgacc cagtctcacc aatcacttgc tcgtgaattt ggtggtatcc
     2701 gtgctggtcg tgccaatgca agcttgcttg accgtgtaca tgtagaatac tatggagtcg
     2761 aaactcctct taaccaaatc gcttcaatta cgattccaga agcgcgtgtt ttgttggtaa
     2821 caccatttga caagtcttca ttgaaagaca tcgaacgtgc cttgaacgct tctgatcttg
     2881 gtatcacacc ggctaatgac ggttctgtga ttcgcttggt tatcccagct cttacagaag
     2941 aaactcgtcg tgaccttgct aaagaagtga agaaggtcgg cgaaaatgct aaagtggctg
     3001 tccgcaatat ccgtcgcgat gctatggacg aagctaagaa acaagaaaaa gcaaaagaaa
     3061 tcactgaaga cgaattgaag actcttgaaa aagacattca aaaagtaaca gacgatgctg
     3121 ttaaacacat cgacgacatg actgctaaca aagagaaaga acttttggaa gtctaaaaat
     3181 aaacagaaaa actcagttgg cattgctggc tgagttttat tcgaaagaag gaaatatgaa
     3241 tacaaatctt gcaagtttta tcgttggact gatcatcgat gaaaacgacc gtttttactt
     3301 tgtgcaaaag gatggtcaaa cctatgctct tgctaaggaa gaaggccaac atacagtagg
     3361 ggatacggtc aagggttttg catacacgga tatgaagcaa aaactccgcc tgacaacctt
     3421 agaagtgact gccactcagg accaatttgg ttggggacgt gtcacagagg ttcgtaagga
     3481 cttgggtgtc tttgtggata caggccttcc tgacaaggaa atcgttgtgt cactcgatat
     3541 tctccctgag ctcaaggaac tctggcctaa gaagggcgac caactctaca tccgtcttga
     3601 agtggataag aaagaccgta tctggggcct cttggcttat caagaagact tccaacgtct
     3661 tgctcgtcct gcctacaaca acatgcagaa ccaaaactgg ccagccattg tttaccgtct
     3721 caagctgtca ggaacttttg tttacctacc agaaaataat atgcttggtt ttattcatcc
     3781 tagcgagcgt tacgcagagc cacgtttggg gcaagtatta gatgcgcgcg ttattggttt
     3841 ccgtgaagtg gaccgcactc tgaacctctc cctcaaacca cgctcctttg aaatgttgga
     3901 aaacgatgct cagatgattt tgacttattt ggaaagtaat ggcggtttca tgaccttaaa
     3961 tgataagtca tctccagacg acatcaaggc aacctttggc atttctaaag gtcagttcaa
     4021 gaaagcctta ggtggtctta tgaaggctgg taaaatcaag caggaccagt ttgggacaga
     4081 gttgatttag ggaggcttat gagaaaatca ttttacactt ggctcatgac cgagcgcaat
     4141 cctaaaagta acagtcccaa agcaattttg gcagacctcg cttttgaaga ggcagctttt
     4201 ccaaaacaca cagatgattt tgatgaggtc agtcgctttt tggaggagca tgccagtttc
     4261 tcttttaacc taggagactt tgacgctatc tggcaagaat acttagaaca ctagcattta
     4321 ttcactaggt ttgggctagt aatttctcca tccctctgct ataataaaaa gaaataaaag
     4381 gattagagag gttctttatt tgaaggaaca ttcaatagac attcaactga gtcatccaga
     4441 tgacctgttt catctttttg gttccaatga acgccatctt cgtttgatgg aagaagagct
     4501 tgatgttgtg attcatgctc gtacggagat tgtccaggtt ttgggagaag agtctgcttg
     4561 cgaggaagcc cgtcaggtta ttcaagctct catggtcttg gtaaatcgtg ggatgaccgt
     4621 tggtacgcca gatgtagtga cggctattag tatggtcaaa aatgatgaaa ttgacaagtt
     4681 tgtcgccctt tacgaagaag aaatcatcaa ggataatact gggaagccta tccgcgtcaa
     4741 aaccttaggt caaaaacttt atgtggacag tgtcaaacag catgatgtga cctttggaat
     4801 tgggccagca ggtacaggga agaccttcct tgcagtgacc ttggcagtga ctgcccttaa
     4861 acgtgggcaa gtcaagcgaa ttatcctaac tcgtccagcg gtggaagcgg gagagagtct
     4921 tggatttctt ccgggtgatc ttaaggagaa ggtggatcct taccttcgtc ctgtttacga
     4981 tgccttgtat caaattcttg ggaaagacca aacgactcgt ctcatggagc gtgaaattat
     5041 cgaaattgcg ccccttgcct atatgcgtgg ccggaccttg gatgatgcct ttgtcattct
     5101 cgatgaggcg caaaacacga ccatcatgca gatgaagatg ttcttgacgc gtttaggctt
     5161 tcattctaag atgattgtca atggagatat cagtcagatt gacctgccac gtaatgtcaa
     5221 gtccggtttg attgatgctc aagagaaact caagaacatc catcagattg actttgttca
     5281 tttttcagcc aaggatgtgg ttcgccatcc agttgtcgct cagattatcc gagcctatga
     5341 atattctact gaagttgcac acgactgatt ttgaggaagt tcgcctgcaa aagaatatac
     5401 ttttgagaga ggaaaatcca gttgtatagg ctaaaggttt tatccaaagg tctgagacaa
     5461 cgattaggca cgatggaaag aacttttatg tgactgatga cgatcagtgc atcttcctgt
     5521 gtcataatca cagggcacaa gaaagtagga atttgaaaag atgattgacc aactatctaa
     5581 gtattacagt tgtaggatac taactgaaaa ggatattcca agtattttat ctttatatga
     5641 aagtaatcct ctgtattttc agcattgtcc accagagcca aattttgcaa ctgtaaaaga
     5701 ggacatgctt tgtctacctg aaggtaaagc taaggctgat aagttttttg ttggattttg
     5761 gaatggatct gaccttgtgg ctgttatgga ttttgtctat gcatatcctg atgaggagac
     5821 tgtttttatt ggttttttta tggttgatca agcctatcag aggaaaggga ttggtagtca
     5881 tattgtgaca gaagcactag cttattttgc taagaacttt cgaaaggcac gtttggctta
     5941 tgttaaggga aatccgcaat ctcagcattt ttgggaaaag cagggcttta aatcaattgg
     6001 atgcgaggtt aagcaagaac tctatacggt tgttatcgtt gaacagagcc tagaagatta
     6061 gaaatggcat caagtaagaa ctatttggaa tttgttttgg aacaattatc aggattagat
     6121 gatgtgactt accgttccat gatgggggag tatattcttt acttccgcgg caagattatt
     6181 ggcggcattt atgacgatcg cttttagtta aacccgtgca agcagtctta gataagattg
     6241 accaatcttc ttttgagttt ccatacaaag gtgccaaaga aatgatttga gtggaagaac
     6301 ttgataataa gatgtttcta taagacctga ttttagctat gtataaccaa ctgccaacgc
     6361 ccaaacctaa aaagaaaaag caagggtgaa cgaagtaaaa aagaagtctg ctaaggccct
     6421 gtctttgcac gggtaaaatt ttatatataa aaagaagctg ggactaaaga gctcagcttc
     6481 ctttggttta tataattgtc attacaagac gaagtggttg ggcgaaactc tgttgacttt
     6541 attcaattta gagtttctta tgcacaattg agtctggaac gaaagtctcc agttgcaaag
     6601 tatacagtac aataaaccaa cgatgtaata gctgatgaca caaagcacag tgggtaggac
     6661 ttgcgaagtc acccttttct tttcaaaatt tatactaaat cattgatatc agtgtagtca
     6721 cgattaagtc cttgagcaac tggtaggcta gtcaagtaac cttgataagt ggtcacacct
     6781 tgacgcaagc cttcatcttc agagattgct tgtgcgaatc ctttgccagc caaagcttcg
     6841 atataaggaa gagtgacatt ggttagggcg atggttgaag tgcgagcaac cgcaccaggg
     6901 atattggcaa cggcatagtg gagaacaccg tgtttttcat agacgggttc atcgtgcgtt
     6961 gtcacacggt cagctgtttc gataacgcca ccttggtcaa cagcaacgtc aacgatacag
     7021 agcctggacg catttgtttg accatctcat ctgtcaccaa ttccggtgct tttgcaccag
     7081 ggatgagaat ggctccaatc accacatcag catctctcat acttgcttca atgttgaatg
     7141 aattagatat aagagtttga atttgacttc caaagacttc ttctagaact gagagacgct
     7201 tggcactaat atctaaaata gtcacttgag caccaagacc aagggcgatg cgggcagcat
     7261 gtgtaccgac gacaccatca ccgatgatag ttacttttcc ttttggaaca cctggtacac
     7321 caccaggtag aacaccagag ccaccagctt gcttagtaag gaagtgagct ccgatttaaa
     7381 cagccatacg acctgcaacc tcactcatag gaacgaggag cggtagttgt ccttgattgt
     7441 cacgaacagt ttcataggca attcctgttg tttttgctgc taacatagca tctgctaatt
     7501 ctggagcagc ggccatgtgc aagtaggtga agagaagaag aacgtcgcgc aagtaaccgt
     7561 attcagaact taaaggttct tttactttca caaccaactc tgctgcccaa gcttcaccag
     7621 cagtagcgac aatctcagct ccttgctttt gatagtcagc atcagtaaag ccagaaccga
     7681 gaccagcatt tgtttcgata aggacacgat gaccacgact aactaagcta tgaacacctg
     7741 caggtgtgag ggcgacacgg ttttcgttat ttttaatttc ttttgggatt ccgattaaca
     7801 ttgagataac ctacctttca attgacggtc ttgttttggt tgtcacattc cagttcataa
     7861 atcaaaaatg tgacggtttc attgtatatg aaaccgcttc aaaaatcaag aaaaacttgt
     7921 catcccaatt tttttatgct agactagtga aaatcaagct ctaatggagg gaaaagtatg
     7981 gaatcaattt ttgtaaaact tgcccagtat ccgtctatag aaacggagcg tttattgctt
     8041 agacctgtaa ctttggatga tgcggaagca atgtttgact atgcctcgga caagggtaat
     8101 acacgttaca cttttccaac caatcaaagc ttggaagaaa ccaagaataa cattgctcag
     8161 ttctatttgg ttaatccctt gggacgttgg ggaatagaac taaaaagcaa tggtcagttt
     8221 attggaacca ttgacttgca caagattgat tctgttctta agaaggcagc tattggctac
     8281 attatcaata aaaagtattg gaatcaagga ttaacgacag aagccaatcg tgctgtgatt
     8341 gagctagctt ttgagaagat agggatgaat aagttgactg cccttcacga taaggctaat
     8401 cccgcgtcag gaaaggtcat ggagaaatca ggcatgcgtt tttcccatgc agaaccatat
     8461 gcttgtatgg accagcatga aaaaggccga atcgtgacaa gagttcatta tgtcttgacc
     8521 aaggaagact attttgcaaa taaataagca gttgaaaaga aatttttcga ctgttttttc
     8581 ttcctcttac gaataatcta agagaggaga aaatatggaa gcaattatcg agaaaatcaa
     8641 agagtataaa atcatcgtca tctgtactgg tctgggcttg cttgtaggcg gatttttcct
     8701 gctaaagcca gctccacaaa cacctgtcaa agaaacgaat ttgcaggttg aagttgcagc
     8761 tgtttccaag gatttggtat ccgaaaagga agtgaacaag gaagagaagg aagaaccagt
     8821 tgaacaagat ctaatcacag tagatgtcaa aggtgctgtc aaatcgccag ggatttatga
     8881 cttgcctgta ggtagtcgag tcaatgatgc tgttcagaag gctggtggct tgacagagca
     8941 agcagacagc aagtcgctca atctagctca gaaagttagt gatgaggctc tggtttacgt
     9001 tcctactaag ggagaagaag cagttagtca acagactggt tcggggacag cttcttcaac
     9061 aagcaaggaa aagaaggtca atctcaacaa ggccagtctg gaagaactca agcaggtcaa
     9121 gggactggga ggaaaacgag ctcaggacat tatcgaccat cgtgaggcaa atggcaagtt
     9181 caagtcagta gacgagctca agaaggtctc tggcattggt ggcaaaacaa tagaaaagct
     9241 taaagactat gttacagtgg attaagaatt tctctattcc cctaatttac ctgagttttc
     9301 tattactttg gctttattac gctattttct cagcatctta tcttgctttg ttgggctttg
     9361 tttttctgct agtctgtctc tttatccaat ttccgtggaa atctgctggt aaagttctaa
     9421 taatttgcgg aatctttgga ttttggtttg tttttcaaaa ttggcaacag agtcaagcga
     9481 gtcaaaatct ggcggattct gttgaaaggg tacggatttt gcctgatact attaaggtta
     9541 atggtgatag tctatccttt cgtggcaagt ctaacggtcg tgctttccaa gtctattata
     9601 aactccagtc cgaggaggag aaagaagcct ttcaagcttt aaccgacctg catgagatag
     9661 gactagaagg gaagctttcg gagccagaag ggcagagaaa ttttggtggc tttaattacc
     9721 aagcctatct gaagactcag ggaatttacc agactctcaa tatcaaaaca atccagtcac
     9781 ttcaaaagat tggcagttgg gatataggag aaaacttgtc cagtttacgt cgaaaggctg
     9841 tggtttggat taagacgcac tttccagacc ctatgcgcaa ttacatgaca ggactcttgc
     9901 tgggacatct ggacaccgac tttgaggaga tgaatgagct ttattccagt ctaggaatta
     9961 tccacctctt tgctctatct ggtatgcagg taggtttttt catgaatgga tttaagaaac
    10021 ttctcttgcg attgggcttg acccaagaaa agttgaaatg gctgacttat cccttttccc
    10081 ttatctatgc gggactaact ggcttttcag catcggttat tcgcagtctc ttgcaaaagc
    10141 tactggctca acatggggtt aagggcttgg ataattttgc cttgacggtg cttgtcctct
    10201 ttattgtcat gccaaacttt ttcttgacag caggaggagt cttgtcctgc gcttatgctt
    10261 ttatcctgac catgaccagc aaagaagggg aggggctcaa ggctgttact agtgaaagtc
    10321 tagtcatctc cttgggcata ttgcccattc tatccttcta ttttgcggaa tttcaacctt
    10381 ggtctatcct tttgaccttt gtcttttcct ttctatttga cttgaccttc ttaccgctct
    10441 tgtctatttt atttgtcctt tcctttctct atccagtcat tcagctgaac tttatctttg
    10501 aatggttgga gggcattatt cgcttggtgt cacaggtgac aagtagacct ctggtctttg
    10561 gacaacccaa tgcatggttt ttaatcctat tgttaatttc cttggctttg gtctatgatt
    10621 tgagaaaaaa cattaaaaag ctaacggtat tgtgcttatt gattacaggg ctctttctcc
    10681 tgaccaagca tccactggaa aatgaaatca ccatgctgga tgtggggcaa ggagaaagta
    10741 ttttcctacg ggatgtaact gggaaaacca ttctcataga tgtaggtggt aaggcagaat
    10801 cttataagaa aatcgaaaaa tggcaagaaa agatgacgac cagcaatgca cagagaagct
    10861 tgatacccta tcttaaaagt cgaggagtag ctaagattga ccagctgatt ttgacaaata
    10921 cggacaagga acatgtcgga gatttgttag aggtgaccaa ggctttccat gtaggggaga
    10981 ttttagtatc aaaaggcagt ttgaagcaga agcaatttgt ggtagaacta caggcgactc
    11041 aaacaaaggt gcgtagtatg acagtagggg agaacttgcc catttttgga agtcagttag
    11101 aagttctatc tccaaggaaa atgggagatg gaggacacga tgatacccta gttctgtatg
    11161 ggaaattctt ggataagcaa tttctcttca cgggaaattt ggaggagaaa ggagagaagg
    11221 acttgctgaa gcactatcca gacttgaaag taaatgtttt gaaagctgcc caacatggct
    11281 ctaaaaagtc atcaagtcca gcctttctag aaaaactcaa accagagctt actcttatct
    11341 cagttggaaa gagcaatcga atgaaactcc cccatcagga aacattgaca cgactggaag
    11401 gtatcaatag caaagtttac cgaactgacc agcaaggagc tatacggttt aaaggttgga
    11461 atagttggaa aatcgaaagt gttcgatagg aaggataaat gttgtagatt agtgaaataa
    11521 actaaaaatt tgttgcataa taatgataaa aatggtataa tgaaaatgta ttcaatactg
    11581 aggatataaa atcattaaaa attagcaaaa atcgtttaat tagttagttt atgattattg
    11641 gtctttttca gtccagtata tctgctgtga cagtcactaa aagttacaag tatgattgga
    11701 atacggtttg ggaatatagt accaactatc acgaccatca gtatgcttgg attccgtcat
    11761 ggtctcgtta ttacagctat tctgagtatc cagttggttc tggctggaac tacgctcgtt
    11821 atgaggtcat aaactactat actggaggtt attaatcctt aaagagtgag aaaaggaggg
    11881 ctggatatgt taaggcttac tcatgttacc ctaaaaacgc gacaagtcat cttgcaagat
    11941 gtggatttca cctttaaaaa gggtagggtt tatggtcttc ttgctatcaa tggctctgga
    12001 aagacgaccc tgttccgtgc cattagcaat ttaattccca taagtagtgg aaatatcgca
    12061 gcccctcctt ctttatttta ttatgagagt attgaatggc tggatggaaa cttaagtggg
    12121 atggactacc ttcgtcttat caaaaacatc tggaagtcag gtctgaactt gagggatgaa
    12181 atcgcctatt gggaaatgtc tgactatatt agtcttccca ttcgcaagta ttccttaggc
    12241 atgaagcaac gcttggtgat tgccatgtat ttcctcagtc aggccaaatg ctggctcatg
    12301 gatgagatta caaatggctt agatgagtat tatcgacaga agttttttga taggctagca
    12361 caaatcgata gacaagaaca gctggttctt ttaagttccc actataagga agagttggtt
    12421 gatgtctgcg atagagtagt aaccattcat caggggcaga tagaagaggt ttagtttatg
    12481 aaagatgtta gtctattttt attgaaaaaa gttttcaaaa gccgcttaaa ctggattgtc
    12541 ttagctttat ttgtatctgt actcggtgtt accttttatt taaatagtca gactgcaaac
    12601 tcacacagct tggagagcag gttggaaagt cgcattgcag ccaacgagag ggctatcaat
    12661 gaaaatgaag agaaactctc ccaaatgtct gatatcagct cggaggaata ccagtttgct
    12721 aaaaataatt tagacgtgca aaaaaatctt ttgaagcgaa agacagaaat tctgacttta
    12781 ttaaaagaag ggcgctggaa agaggcctac tatctgcagt ggcaagatga agagaagaat
    12841 tatgaatttg tatcaaatga cccgactgct agctctggct taaaaatggg ggttgaccgc
    12901 gaacggaaga tttaccaagc catgtatccc ttgaacataa aagcacatac tttggagttt
    12961 ccgacccacg ggattgatca gattgtctgg attttagagg ttatcatccc aagcttgttt
    13021 gtggttgcga ttatttttat gctaacacaa ctatttgcag aaagatatca aaatcatctg
    13081 gacacagctc acttatatcc tgtttcaaaa gtgacatttg caatatcctc tcttggagtt
    13141 ggagtgggat atgtaactgt gctgtttatc ggaatctgtg gcttttcttt tctagtggga
    13201 agtctgataa gtggttttgg acagttagat tatccctacc caatttatag cttagtgaat
    13261 caagaagtaa ctattgggaa aatacaagat gtattatttc ctggcttgct cttagctttc
    13321 ttagccttta tcgtcattgt ggaagttgtg tacttgattg cttacttttt caagcaaaaa
    13381 atgcctgtcc tctttctttc actcattggg attgttggct tattgtttgg catccaaacc
    13441 attcagcctc ttcaaaggat tgcacatctg attcccttta cttacttgcg ttcagtggag
    13501 attttatctg gaagattatc taagcagatt gataatgtcg atctaaattg gagcatgggg
    13561 atggtcttac ttccttgcct gattatcctt ttgttagtgg ggattctatt tatcgaaaga
    13621 tggggaagtt cacgtaaaaa ggaagttttt aaagtttagt tttcctatag ggaagctaaa
    13681 gaaaaactaa catagggaga gaatcaactt ggttctctct ttttgattcg aaaaccaaac
    13741 caaaatacaa acacaaactt ttcaaaaaat aactttttat cttgacaaga gctagaaaac
    13801 ttggtatcat ataaaagttg agaaaagcag aagtgagagc ttctcgcctt gtgacattaa
    13861 gttgcctggc cctacggatg aaaagtttcg aagaaacgct atcataacgt gcgggcttgt
    13921 atatttacga gtccgctatt gtttttctct aataaaacaa aagaggtgaa aaccatagca
    13981 aagcaagact tattcatcaa tgatgagatt cgtgtacgtg aagttcgctt gattggtctt
    14041 gagggagaac agctaggcat caagccactc agtgaagcgc aagctttggc tgataacgct
    14101 aatgttgacc tagtattgat tcaaccccaa gccaaaccgc ctgttgcaaa aattatggac
    14161 tacggtaagt tcaaatttga gtaccagaag aagcaaaaag aacaacgtaa aaaacaaagc
    14221 gttgttactg tgaaagaagt tcgtctaagt ccaacaattg acaagggtga ctttgataca
    14281 aaacttcgca atgcacgtaa gttccttgaa aaaggaaata aagttaaggt atctattcgc
    14341 tttaagggtc gtatgattac ccacaaagag attggtgcaa aagttttagc cgagtttgct
    14401 gaagcaactc aagatattgc catcatcgaa caacgtgcta aaatggatgg acgtcaaatg
    14461 ttcatgcaat tggcgccagc gactgacaaa aaataattgt cagaaagtta aataaaggag
    14521 aaaaaatcat gccaaaacaa aaaacacacc gcgcatcagc taaacgtttc aaacgtacag
    14581 gttctggtgg acttaaacgt ttccgtgctt acacttctca ccgtttccac ggaaaaacta
    14641 agaaacaacg tcgtcatctt cgtaaagcat ctatggtgca ttcaggagat tacaaacgta
    14701 tcaaagcaat gcttactcgc ttgaaataat agcttgactg taagtaaaca attctaggaa
    14761 atatttggag gaaataaaac atggcacgtg ttaaaggtgg cgttgtatca cgcaaacgtc
    14821 gtaaacgtat tcttaaatta gcaaaaggtt actatggagc taaacacatc ttgttccgta
    14881 ctgcaaaaga acaagtaatg aactcttact actatgcata ccgtgaccgt cgtcagaaaa
    14941 aacgtgactt ccgcaaattg tggattactc gtatcaatgc ggcagctcgt atgaacggac
    15001 tttcatactc acaattgatg catggtttga aattggctga gattgaagtt aaccgtaaaa
    15061 tgcttgctga cttggctgtt aacgatgcag tagctttcac agctcttgca gatgcagcta
    15121 aagcaaaact tggtaaataa caactgatga gactggaaca ggattgtcca gtctttttaa
    15181 aaataaagga gaaagatatg gcttcaaaaa tgctacacac ttgcttacga gtagaaaatc
    15241 ttgaaaaatc aatcgcattt tatcaagatg cttttggttt taaagaattg cgtcgcagag
    15301 attttccaga ccatgccttc acgattgtct atctaggtct tgagggtgat gattatgagt
    15361 tggagttgac ttataactac gatcacggtc cgtatgtggt tggtgatggg tttgcccata
    15421 tcgccctcag tacacctgat cttgaggcac ttcatcaaga acacagtgca aaaggctatg
    15481 aagtgactga gccaaatggt ctaccaggaa ctacaccaaa ctattacttt gtcaaagacc
    15541 ctgatggata taaggtcgaa gtcattcgtg aaaaataatt tcgtgcaagt taagtagaat
    15601 attcgttttc tacttgactt tttttagctg aaagagtata ctaataaaaa ttcaaccttt
    15661 aaggggagtc cagagagact cacaaggtgt cagataaaag aatagtgcaa ttttctagag
    15721 gagacctttt gagtgtgctc tcttgtgttg tacgatttta actgaggcct tgcactagca
    15781 aggtcttttc tttatctggt ccccttaaaa tttaaggagg aaaagttatg aatctcacat
    15841 gtaagaagcg tttgggtgtc attcggttgg aaaccatgaa ggtggttgca caagaggaaa
    15901 tcgcgccagc aatctttgaa ttagtcctag aaggagaaat ggttgaagcc atgcgagcag
    15961 gccaatttct tcatctgcgt gtaccagacg atgcccatct cttacgtcgt cctatttcaa
    16021 tttcgtctat tgacaaggca aacaagcagt gtcacctcat ttatcggatt gagggagctg
    16081 ggactgcaat tttttcaacc ttaagtcagg gagacactct tgatgtgatg gggcctcagg
    16141 gaaatggttt tgacttgtct gaccttgatg agcagaatca ggttctcctt gttggtggtg
    16201 ggattggtgt tccacccttg cttgaggtgg ccaaggaatt gcatgaacgt ggagtgaaag
    16261 tagtgacagt cctcggtttt gctaataagg atgctgttat tttgaaaacg gaattggctc
    16321 agtatggtca ggtctttgta acgacagatg atggttctta tggcatcaag ggaaatgttt
    16381 ccgttgttat caatgattta gatagtcagt ttgatgctgt ttactcgtgt ggggctccag
    16441 gaatgatgaa gtatatcaat caaacctttg atgatcaccc aagagcctat ttatctctgg
    16501 aatctcgtat ggcttgtggg atgggagctt gctatgcctg tgttctaaaa gtaccagaaa
    16561 gcgagacggt aagccaacgc gtctgtgaag atggtcctgt tttccgcaca ggaacagttg
    16621 tattataagg agaaaattat gactacaaat cgattacaag tgtctctacc tggtttggat
    16681 ttgaaaaatc cgattattcc agcatcaggc tgttttggct ttggacaaga gtatgccaag
    16741 tactatgatt taaacctttt aggttctatt atgatcaagg cgacaaccct tgaaccacgt
    16801 tttgggaacc caactccaag agtagcagag acgcctgctg gtatgctcaa tgcaattggc
    16861 ttgcaaaatc ctggtttaga ggttgttttg gctgaaaagc taccttggct ggaaagagaa
    16921 tatccaaatc ttcctattat tgccaatgta gctggttttt caaaacaaga gtatgcagct
    16981 gtttctcatg ggatttccaa ggcaactaat gtaaaagcta tcgagctcaa tatttcttgt
    17041 cccaatgttg accactgtaa tcatggactt ttgattggtc aagatccaga tttggcttat
    17101 gatgtggtga aagcagctgt ggaagcctca gaagtgccag tttatgtcaa attaaccccg
    17161 agtgtgaccg atatcgttac tgtcgcaaaa gctgcagaag atgcgggagc aagtggcttg
    17221 accatgatca atactctggt tggaatgcgc tttgacctca aaaccagaaa accaatcttg
    17281 gccaatggaa caggtggaat gtcaggtcca gcagtctttc cagtagccct caaactcatc
    17341 cgccaagtag cccaaacaac agacctgcct atcattggaa tggggggagt ggattcggct
    17401 gaagctgccc tagaaatgta tctggctggg gcagctgcta tcggagttgg aacagctaac
    17461 tttaccaatc cttatgcctg ccctgacatc atcgaaaatt taccaaaagt catggataaa
    17521 tacggtatta gcagtctgga agaactccgt caggaagtaa aagagtctct gaggtaaact
    17581 gcaatcaatc tgttcttgat tttttattag tttgtaatat gaatttagga gaattttggt
    17641 acaataaata taaataagaa cagaggaaga aggttaatga agaaagtaag atttattttt
    17701 ttagctctgc tatttttctt agctagtcca gagggtgcaa tggctagtga tggtacttgg
    17761 caaggaaaac agtatctgaa agaagatggc agtcaagcag cgaatgagtg ggtttttgat
    17821 actcattatc aatcttggtt ctatataaaa gcagatgcta actatgctga aaatgagtgg
    17881 ctaaagcaag gtgacgacta tttttacctc aaatctggtg gctatatggc caaatcagaa
    17941 tgggtagaag acaagggagc cttttattat cttgaccaag atggaaagat gaaaagaaat
    18001 gcttgggtag gaacttccta tgttggtgca acaggtgcca aagtaataga agactgggtc
    18061 tatgattctc aatacgatgc ttggttttat atcaaagcag atggacagca cgcagagaaa
    18121 gaatggctcc aaattaaagg gaaggactat tatttcaaat ccggtggtta tctactgaca
    18181 agtcagtgga ttaatcaagc ttatgtgaat gctagtggtg ccaaagtaca gcaaggttgg
    18241 ctttttgaca aacaatacca atcttggttt tacatcaaag aaaatggaaa ctatgctgat
    18301 aaagaatgga ttttcgagaa tggtcactat tattatctaa aatccggtgg ctacatggca
    18361 gccaatgaat ggatttggga taaggaatct tggttttatc tcaaatttga tgggaaaata
    18421 gctgaaaaag aatgggtcta cgattctcat agtcaagctt ggtactactt caaatccggt
    18481 ggctacatgg cagccaatga atggatttgg gataaggaat cttggtttta cctcaagttt
    18541 gatggcaaaa tggctgaaaa agaatgggtc tacgattctc atagtcaagc ctggtactac
    18601 ttcaaatccg gtggttacat gacagccaat gaatggattt gggataagga atcttggttt
    18661 tatctcaaat ctgatgggaa aatagctgaa aaagaatggg tctacgattc tcatagtcaa
    18721 gcttggtact acttcaaatc cggtggttac atgacagcca atgaatggat ttgggataag
    18781 gaatcttggt tttacctcaa gtctgatggc aaaatggctg aaaaagaatg ggtctacgat
    18841 tctcatagtc aagcctggta ctacttcaaa tctggtggct acatggcgaa aaatgagaca
    18901 gtagatggtt atcagcttgg aagcgatggt aaatggcttg gaggaaaagc tacaaataaa
    18961 aatgctgctt actatcaagt agtgcctgtc acagccaatg tttatgattc agatggtgaa
    19021 aagctttcct atatatcgca aggtagtgtc gtatggctag ataaggatag aaaaagtgat
    19081 gacaagcgct tggctattac tatttctggt ttgtcaggct atatgaaaac agaagattta
    19141 caagcgctag atgctagtaa ggactttatc ccttattatg agagtgatgg ccaccgtttt
    19201 tatcactatg tggctcagaa tgctagtatc ccagtagctt ctcatctttc tgatatggaa
    19261 gtaggcaaga aatattattc ggcagatggc ctgcattttg atggttttaa gcttgagaat
    19321 cccttccttt tcaaagattt aacagaggct acaaactaca gtgctgaaga attggataag
    19381 gtatttagtt tgctaaacat taataatagc cttttggaga acaagggcgc tacttttaag
    19441 gaagccgaag aacattacca tatcaatgcc ctttatctcc ttgcccatag tgccctagaa
    19501 agtaactggg gaagaagtaa aattgccaaa gataagaata atttctttgg cattacagcc
    19561 tatgatacga ccccttacct ttctgccaag acatttgatg atgtggataa gggaatttta
    19621 ggtgcaacca agtggattaa ggaaaattat atcgataggg gaagaacttt ccttggaaac
    19681 aaggcttctg gtatgaatgt ggaatatgct tcagaccctt attggggcga aaaaattgct
    19741 agtgtgatga tgaaaatcaa tgagaagcta ggtggcaaag attagtacta taagtgaata
    19801 tgatttgagt gaatagtaag ttaaaaatcc tgatttcaag taaaatcagg attttttcat
    19861 ggatgcaatt tttttggagt ctggtgtgac gcggagggtc ttttgtcctg tgtaagtgac
    19921 aaagccgggt tttccaccag ttggtttatt gagttttttg acttcaatca tatctacctg
    19981 caccagattc gacaggcgcc cttgagagaa gtaggcagct aactctgctg cgtctgtctt
    20041 gactgcatca gatgggtcaa gatttcctga gatgacaaca tggcttccag gaatgtcctt
    20101 agcatggaac caaagttcct ccttgcgggc cattttaaag gtcaattcct cattttgaag
    20161 attgtttcgt ccgacataga tgatggtttt gccatcgctt gctagatatt gttctagttt
    20221 tttgcgtttc tggattttct cccgttgtct tctgcggata aaacctgttt gaatcaattc
    20281 ttcacggatt tcagcgattt cttccagtcc agcttggttg aggacggttt ctacactttc
    20341 cagatagaga atagtggctt tggtttcttc aatcaaatca gtcaagtatt tgacagcttc
    20401 tttgagtttc tgataccgtt taaaatagcg ttgggcattc tggttgggag tcagagcctt
    20461 atcaagcgca atcatgatag gttggttggt atagtagttg tctaggataa cctggtcttg
    20521 gtcgttaggc acttggtgta ggaaggttgt cagcaattct cctttttgac gaaattcttc
    20581 agcgttgtct gtcgccagta actctttttc ctgttttttg agtttgtgtc ggtttttctg
    20641 aagttcattt tcaacacgac gaatcagttc actggcctgc tgtttgacgc ggtcgcgctc
    20701 agccttatcc ttatagtagg tgtccaacaa atcagaaaga tttgcaaaag gctctcccac
    20761 ctgatttgca aaaggaactg gactgaagga agtctcagtc aagcatggct tggtttcttg
    20821 attgaaaaaa tttcggaaag cggaaagttt ttcactaacc agtatccttt ccaattcatt
    20881 tgcagtatcg cgtcccagac cttgaaagag gctttgaaga ttttttgctg ttagttcttg
    20941 ggtttgcagg atttcaaaga gcttttcatc tttgatagta aaaggattga gagattctgt
    21001 acttggcgga gcgatatagg tcgatcctgg aagtaaggtg cggtagctat tttgtgaaaa
    21061 accgacgtgt ttgataactt cgaggatttt atgactgctt ttatcgacca gtagaatatt
    21121 actgtgtttc cccataattt caataatcaa ggtagcctgg atatggtctc caatctcgtt
    21181 tttattggaa actgtcattt ccacaatacg gtcattttcc acttgctcaa tcgactcaat
    21241 cagggccccc tgcaaatact ttctcaaaac catgataaag gtagaaggtt gggctggatt
    21301 ttcaaaagtc gtttgggtca gctgaatgcg tccaaaaact ggatgggcag aaaggagcag
    21361 gcgatggctt tggcgattgc tgcggatttg caagaccaac tcttgttcaa aaggctgatt
    21421 gattttctgg atgcgaccat tcactaattc gcttcgcaat tcctcaacta tgtggtgtaa
    21481 aaaaaatcca tcaaatgaca tcgttctctc cttgtgattg tattccatag tattatatca
    21541 aaaaggtaga ataaaatcat ggaaatgtgg tataataaag ccaagtaaag agaaacgaga
    21601 agcacatgta tattgaaatg gtagatgaaa ctggtcaagt ttcaaaagaa atgttgcaac
    21661 aaacccaaga aattttggaa tttgcagccc aaaaattagg aaaagaagac aaggagatgg
    21721 cagtcacttt tgtgaccaat gagcgtagtc atgaacttaa tctggagtac cgtgacaccg
    21781 accgtccgac agatgtcatc agccttgagt ataaaccaga attggaaatt gcctttgacg
    21841 aagaggattt gcttgaaaat ccagaattgg cagagatgat gtctgagttt gatgcctata
    21901 ttggggaatt gttcatctct atcgataagg ctcatgagca ggccgaagaa tatggtcaca
    21961 gctttgagcg tgagatgggc ttcttggcag tacacggctt tttacatatt aacggctatg
    22021 atcactacac tccggaagaa gaagcggaga tgttcggttt acaagaagaa attttgacag
    22081 cctatggact cacaagacaa taaacgaaaa tggaaaaatc gtgacctgat atccagttta
    22141 gaatttgctt tgacaggtat ttttactgct atcaaggaag aacgaaatat gcgaaaacat
    22201 gcagtgacgg ctctagtggt tatccttgca ggttttgttt ttcaggtgtc acgaatcgaa
    22261 tggctctttc tcctattgag cattttcttg gtagtagcct ttgagattat caactctgct
    22321 attgaaaata tggtggattt ggccagtcac tatcactttt ccatgctggc taaaaatgcc
    22381 aaggatatgg cggctggtgc ggtattagtg gtttctcttt tcgcagcctt aacaggcgca
    22441 ttgatttttc tcccgcgaat ctgggattta ttattttaaa cagtaagagg aaattatgac
    22501 atttaaatca ggctttgtag ccattttagg acgtcctaac gttgggaagt caaccttttt
    22561 aaatcacgtc atggggcaaa agattgccat catgagtgac aaggcgcaga caacgcgcaa
    22621 caaaatcatg ggaatttaca cgactgataa ggagcaaatt gtctttatcg acacaccagg
    22681 gattcacaaa cctaaaacag ctctcggaga tttcatggtt gagtctgcct acagtaccct
    22741 tcgcgaagtg gacaccgttc ttttcatggt gcctgctgat gaagcgcgtg gtaaggggga
    22801 cgatatgatt atcgagcgtc tcaaggctgc caagattcct gtgattttgg tggtgaataa
    22861 aatcgataag gtccatccag accagctctt gtctcagatt gatgacttcc gtaatcaaat
    22921 ggactttaag gaaattgttc caatctcagc ccttcaggga aataacgtgt ctcgtctagt
    22981 ggatattttg agtgaaaatc tggatgaagg tttccaatat ttcccgtctg atcaaatcac
    23041 agaccatcca gaacgtttct tggtttcaga aatggttcgc gagaaagtct tgcacctaac
    23101 tcgtgaagag attccgcatt ctgtagcagt agttgttgac tctatgaaac gagacgaaga
    23161 gacagacaag gttcacatcc gtgcaaccat catggtcgag cgcgatagcc aaaaagggat
    23221 tatcatcggt aaaggtggcg ctatgcttaa gaaaatcggt agcatggccc gtcgtgatat
    23281 cgaactcatg ctaggagaca aggtcttcct agaaacctgg gtcaaggtca agaaaaactg
    23341 gcgcgataaa aagctagatt tggctgactt tggctataat gaaagagaat actaagtaga
    23401 ggtaggctca tgcctgcttc ttgtttttac agaaggagga cttatgcctg aattacctga
    23461 ggttgaaacc gtttgtcgtg gcttagaaaa attgattata gggaagaaga tttcgagtat
    23521 agaaattcgc taccccaaga tgattaagac ggatttggaa gagtttcaaa gggaattgcc
    23581 tagtcagatt atcgagtcaa tgggacgtcg tggaaaatat ttgctttttt atctgacaga
    23641 caaggtcttg atttcccatt tgcggatgga gggcaagtat ttttactatc cagaccaagg
    23701 acctgaacgc aagcatgccc atgttttctt tcattttgaa gatggtggca cgcttgttta
    23761 tgaggatgtt cgcaagtttg gaaccatgga actcttggtg cctgaccttt tagacgccta
    23821 ctttatttct aaaaaattag gtcctgaacc aagcgaacaa gactttgatt tacaggtctt
    23881 tcaatctgcc cttgccaagt ccaaaaagcc tatcaaatcc catctcctag accagacctt
    23941 ggtagctgga cttggcaata tctatgtgga tgaggttctc tggcgagctc aggttcatcc
    24001 agctagacct tcccagactt tgacagcaga agaagcgact gccattcatg accagaccat
    24061 tgctgttttg ggccaggctg ttgaaaaagg tggctccacc attcggactt ataccaatgc
    24121 ctttggggaa gatggaagca tgcaggactt tcatcaggtc tatgataaga ctggtcaaga
    24181 atgtgtacgc tgtggtacca tcattgagaa aattcaacta ggcggacgtg gaacccactt
    24241 ttgtccaaac tgtcaaagga gggactgatg ggaaaaatca tcggaatcac tgggggaatt
    24301 gcctctggta agtcaactgt gacaaatttt ctaagacagc aaggctttca agtagtggat
    24361 gccgacgcag tcgtccacca actacagaaa cctggtggtc gtctgtttga ggctctagta
    24421 cagcactttg ggcaagaaat cattcttgaa aacggagaac tcaatcgccc tctcctagct
    24481 agtctcatct tttcaaatcc tgaagagcaa aaatggtcta agcaaattca aggggagatt
    24541 atccgtgagg aactggctac tttgagagaa cagttggctc agacagaaga gattttcttc
    24601 atggatattc ccctactttt tgagcaggac tacagcgatt ggtttgctga gacttggttg
    24661 gtctatgtgg accgagatac ccaagtagaa cgcttaatga aaagggacca gttgtccaag
    24721 gatgaagctg agtctcgtct ggcagcccag tggcctttag aaaaaaagaa agatttggcc
    24781 agccaggttc ttgataataa tggcaatcag aaccagcttc ttaatcaagt gcatatcctt
    24841 cttgagggag gtaggcaaga tgacagagat taactggaag gataatctgc gcattgcctg
    24901 gtttggtaat tttctgacag gagccagtat ttctttggtt gtacctttta tgcccatctt
    24961 cgtggaaaat ctaggtgtag ggagtgagca agtcgctttt tatgcaggct tagcaatttc
    25021 tgtctctgct atttccgcgg cgctcttttc tcctatttgg ggtattcttg ctgacaaata
    25081 cggccgaaaa cccatgatga ttcgggcagg tcttgctatg actatcacta tgggaggctt
    25141 ggcctttgtc ccaaatatct attggttaat ctttcttcgt ttactaaacg gtgtatttgc
    25201 aggttttgtt cctaatgcaa cggcactgat agccagtcag gttccaaagg agaaatcagg
    25261 ctctgcctta ggtactttgt ctacaggcgt agttgcaggc actctaactg gtccctttat
    25321 tggtggcttt atcgcagaat tatttggcat tcgtacagtt ttcttactgg ttggtagttt
    25381 tctattttta gctgctattt tgactatttg ctttatcaag gaagattttc aaccagtagc
    25441 caaggaaaag gctattccaa caaaggaatt atttacctcg gttaaatatc cctatctttt
    25501 gctcaatctc tttttaacca gttttgtcat ccaattttca gctcaatcga ttggccctat
    25561 tttggctctt tatgtacgcg acttagggca gacagagaat cttctttttg tctctggttt
    25621 gattgtgtcc agtatgggct tttccagcat gatgagtgca ggagtcatgg gcaagctagg
    25681 tgacaaggtg ggcaatcatc gtctcttggt tgtcgcccag ttttattcag tcatcatcta
    25741 tctcctctgt gccaatgcct ctagccccct tcaactagga ctctatcgtt tcctctttgg
    25801 attgggaacc ggtgccttga ttcccggggt taatgcccta ctcagcaaaa tgactcccaa
    25861 agccggcatt tcgagggtct ttgccttcaa tcaggtattc ttttatctgg gaggtgttgt
    25921 tggtcccatg gcaggttctg cagtagcagg tcaatttggc taccatgctg tcttttatgc
    25981 gacgagcctt tgtgttgcct ttagttgtct ctttaacctg attcaatttc gaacattatt
    26041 aaaagtaaag gaaatctagt gcgagtaaaa attaatctca aatgctcctc ttgtggcagt
    26101 atcaattacc taaccagtaa aaattcaaaa acccatccag acaagattga ggttttaaag
    26161 tattgtccca aggaaagaaa agtaacccta catcttgaat ctaagtagat tttatggtaa
    26221 aataataggg attttaagga gtttgatatg tataacctat tattaaccat tttattagta
    26281 ttatctgttg tgattgtgat tgcaattttc atgcaaccaa ccaaaaacca atccagcaat
    26341 gtatttgatg ccagttcagg tgatttgttt gaacgcagta aagctcgcgg ttttgaagct
    26401 gtaatgcagc gtttgacagg gattttagtc tttttctggc tagccattgc cttagcattg
    26461 acggtattat caagtagata agaaaataat gggcaggact aggtctttgc ctctttttat
    26521 ttttaaagga tgtttgagaa ggttttacag taaaagaaaa ttaaaaaatc tagaaagaaa
    26581 atatgaaaga tagaataaaa gaatatttac aagacaaggg aaaggtgact gttaatgatt
    26641 tggctcaggc tttgggaaaa gacagttcca aggattttcg tgagttgatt aaaaccttgt
    26701 ccttaatgga aagaaagcac caaattcgtt ttgaagaaga tggtagtctg acattagaaa
    26761 ttaagaaaaa acatgagatt accctcaagg ggatttttca tgcccataaa aatggctttg
    26821 gctttgttag tctggaaggc gaggaggacg acctttttgt agggaaaaat gatgtcaact
    26881 atgctattgc tggtgatacc gtcgaggtag tgattaagaa agtcgctgac cgcaataagg
    26941 gaacagcagc agaagccaaa attattgata tcctagaaca cagtttgaca acagttgtcg
    27001 ggcaaatcgt tctggatcag gaaaaaccta agtatgctgg ctatattcgt tcaaaaaatc
    27061 agaaaatcag tcaaccgatt tatgttaaga aaccagccct aaaattagaa ggaacagaag
    27121 ttctcaaagt ctttatcgat aaatacccaa gcaagaaaca tgatttcttt gtcgcgagtg
    27181 ttctcgatgt ggtgggacac tcaacggatg tcggaattga tgttcttgag gttttggaat
    27241 caatggacat tgtatccgag tttccagaag ctgttgttaa ggaagcagaa agtgtgcctg
    27301 atgctccgtc tcaaaaggat atggaaggtc gtctggatct aagagatgaa attaccttta
    27361 ccattgacgg tgcggatgcc aaggacttgg acgatgcagt gcatatcaag gctctgaaaa
    27421 atggcaatct ggagcttggg gttcacatcg cagatgtttc ttattatgtg accgaggggt
    27481 ctgcccttga caaggaagcc cttaaccgtg cgacttctgt ttacgtgaca gaccgagtgg
    27541 tgccaatgct tccagaacga ctatcaaatg gcatctgctc tctcaatccc caagttgacc
    27601 gcctgaccca gtctgctatt atggagattg ataaacatgg tcgtgtggtc aactatacca
    27661 ttacacaaac agttatcaag accagttttc gtatgaccta tagcgatgtc aatgatatcc
    27721 tagctggcga tgaagaaaag agaaaagaat atcataaaat tgtaccaagt atcgaactca
    27781 tggccaagct tcatgaaact ttagaaaaca tgcgtgtgaa acgtggagct ctcaattttg
    27841 ataccaatga agcgaagatt ttagtggata aacaaggtaa gcctgttgat atcgttcttc
    27901 ggcagcgtgg tattgccgag cggatgattg agtcttttat gttgatggct aatgaaacag
    27961 ttgccgaaca tttcagcaag ttggatttgc cttttatcta tcgaattcac gaggagccta
    28021 aggctgaaaa ggttcagaag tttattgatt atgcttcgag ttttggcttg cgcatttatg
    28081 ggactgccag tgagattagc caggaggcac ttcaagacat catgcgtgct gttgagggag
    28141 aaccttatgc agatgtattg tccatgatgc ttcttcgctc tatgcagcag gctcgttatt
    28201 tggagcacaa tcacggccac tatggactag ctgctgacta ttatactcac tttaccagtc
    28261 caattcgtcg ttatccagac cttcttgttc accgtatgat tcgggattac ggccgttcta
    28321 aggaaatagc agagcatttt gaacaagtga ttccagagat tgcgacccag tcttccaacc
    28381 gtgaacgtcg tgccatagaa gctgagcgtg aagtcgaagc catgaaaaag gctgagtata
    28441 tggaagaata cgtgggtgaa gagtatgatg cagttgtatc aagtattgtc aaattcggtc
    28501 tctttgtcga attgccaaac acagttgaag gcttgattca catcactaat ctacctgaat
    28561 tttatcattt caatgagcgt gatttgactc ttcgtggaga aaaatcaggt atcactttcc
    28621 gagtgggtca gcagatccgt atccgtgttg aaagagcgga taaaatgact ggagagattg
    28681 atttttcatt cgtacctagt gagtttgatg tgattgaaaa aggcttgaaa cagtctagtc
    28741 gtagtggcag agggcgtgat tcaaatcgtc gttcggataa gaaggaagac aagagaaaat
    28801 caggacgctc aaatgataag cgtaagcatt cacaaaaaga caagaagaaa aaaggaaaga
    28861 aaccttttta caaggaagta gctaagaaag gagccaagca tggcaaaggg cgagggaaag
    28921 gtcgtcgcac aaaataaaaa ggcacgccac gactatacaa tcgtagatac gctagaggca
    28981 gggatggtcc tgactggaac tgaaatcaag agtgtacgag ctgctcgaat taatctcaag
    29041 gatggctttg ctcaagtgaa aaatggagaa gtttggctga gcaatgttca tatcgcgcct
    29101 tacgaagagg gcaatatctg gaaccaggaa ccagaacgtc gtcgtaaact cctgctccat
    29161 aaaaggcaaa ttcaaaaatt ggaacaagag accaaaggga caggaatgac cttagttccc
    29221 cttaaggtct atataaaaga tggctacgct aagcttcttt taggacttgc caaagggaag
    29281 catgactatg acaaacggga gtctatcaaa cgtcgtgagc aaaatcgaga tatcgcgcgt
    29341 gtgatgaaag ctgttaatca gcgataaaaa gaggaattga aaatggaaaa attagttgcc
    29401 tataaacgca tgcctttgtg gaataaacaa acaatgcctg aagctgttca gcaaaagcac
    29461 aatacaaaag ttgggacttg ggggaaaatt actgtcttga agggagctct caagtttatt
    29521 gaattgacag aagaagggga agttctagct gaacacctct ttgaagcagg ggcagacaat
    29581 ccaatggccc aacctcaagc ctggcaccga gtggaagctg ccacagatga tgtggaatgg
    29641 tacttggaat tttattgtaa acctgaggat tattttgcta aaaaatacaa taccaatcct
    29701 gttcattcag aggtcctaga ggccatggag acagtgaaac aagggaaagc tttggatttg
    29761 ggttgtggtc aggggcgtaa ttctcttttt ctagcccagc aagattttga tgtgacggct
    29821 gtagatcaaa atggactagc tcttgaaatc ttgcaaagca ttgtggagca ggaagatttg
    29881 gacatgcctg ttggccttta cgatatcaat tcagctagca ttgaacaaga atatgatttt
    29941 atcgtttcaa cagttgttct catgtttcta caagcggacc gcattccagc tattattcaa
    30001 aatatgcagg agaaaaccag tgttggtggt tacaacctta tcgtttgtgc catggacacg
    30061 gaggattatc cttgctcggt taacttccca ttcaccttta aagaaggaga actggcagac
    30121 tattacaagg attgggaatt ggttaagtac aatgaaaatc caggccattt gcatcgtcgc
    30181 gatgagaatg gcaatcgtat tcaactacgc tttgcgacct tactagctaa gaaaatcaag
    30241 taaacacaca tgaagattag gaattttcct gatctttttt cttttttacg aatgatatag
    30301 aaaaggaggg aattcatgtt tgttgcgaga gatgctaggg gagaattggt aaatgtgtta
    30361 gaggataaac ttgagaagca agcatacacc tgcccagctt gtggaggcca gctccatttg
    30421 cgtcaaggac caagtgtacg gacgcatttt gcccataaat ccttaaaaga ctgtgatttt
    30481 ttctttgaaa atgaaagtcc agaacacctg gccaataagg aatccctcta tcactggttg
    30541 aaaaaagaga caaaggttca attagagtac ccgctttcag aacttaaaca gattgcggat
    30601 gtatttgtaa atggcaatct agctctagaa gttcagtgta gtcccttgcc tcagaaagtc
    30661 cttaaagagc gaagtgaggg ctatcgtagt cagggttacc aagtactgtg gttgctgggt
    30721 caaaaactgt ggctcaagga gcgtttgact cgtctacagc aaggttttct ttatttcagt
    30781 caaaacatgg gcttttatgt ttgggaatta gacaaggaaa aacaagtttt aagactcaaa
    30841 tacctgattt accaggatct ccgcggtaaa ctccattatc aaatcaagga attttcctat
    30901 ggtcaaggta gtttattgga aatattgcgt cttccctata agagacaaaa aatatctcat
    30961 tttacagttt ctgaggacaa ggacatctgt cgctatatcc agcaacaact ttattatcaa
    31021 aatctctttt ggatgaaaga acaagcagaa gcctatcaaa agggagaaaa tatcctgact
    31081 tatggactga aagaatggta tccacaaatt cgaccaatag tgggcaaatt tttccagatt
    31141 gaacaagact tgactagcta ttatcagcac ttttatacct attaccaaaa aaatcctcaa
    31201 aatgattggc aaaagcttta tccaccagcc ttttatcagc aatatttctt gaaaaatatg
    31261 gtagaataga aaggatggag gaatctaatg gtattacaaa gaaatgaaat aaatgaaaaa
    31321 gatacatggg atctatcaac gatctaccca actgaccagg cttgggaaga agccttaaaa
    31381 gatttaacag aacaattgga gacagtagcc cagtatgaag gccatctctt ggatagtgcg
    31441 gataacctac tagaaatcac tgaattttct cttgaaatgg aacgccagat ggagaagctt
    31501 tacgtttatg ctcatatgaa gaatgaccag gatacacgtg aagctaagta tcaagagtac
    31561 tatgccaagg ccatgacact ctacagccag ttagaccaag ccttttcatt ctatgatcct
    31621 gaatttatgg agattagcga aaagcagtat gctgactttt tagaagctca accaaagctg
    31681 caggtttatc aacactattt tgacaagctc ttgcaaggca aggatcacgt tctttcacaa
    31741 cgtgaagaag aattattggc tggagctgga gaaatctttg gttcagcaag tgaaaccttc
    31801 gctatcttgg acaatgcgga tattgtgttc ccttatgtcc tagacgatga tggtaaagaa
    31861 gttcagctat ctcatgggac ttacacacgt ttgatggagt ctaaaaaacg tgaggttcgc
    31921 cgtggtgcct atcaagctct ttatgcgact tacgaacaat tccaacacac ctatgccaaa
    31981 accttgcaaa ccaatgttaa ggtgcaaaat taccgtgcta aagttcgtaa ctacaagagt
    32041 gctcgtcatg cagccctcgc agcgaatttt gttccagaaa gtgtttatga caatttggta
    32101 gcagcagttc gcaagcattt gccactctta catcgctatc ttgagcttcg ttcaaaaatc
    32161 ttggggattt cagatctcaa gatgtacgat gtctacacac cgctttcatc tgttgaatac
    32221 aattttacct accaagaagc cttgaaaaaa gcagaagatg ctttggcagt cttgggtgag
    32281 gattacttga gccgtgtcaa acgtgccttc agcgagcgtt ggattgatgt ttacgaaaat
    32341 caaggcaagc gttcaggtgc ctactctggt ggttcttacg ataccaatgc ctttatgctt
    32401 ctcaactggc aggacaatct ggacaatctc tttactcttg ttcatgaaac aggtcacagt
    32461 atgcattcaa gctatactcg tgaaactcag ccttatgttt acggagatta ctctatcttt
    32521 ttggctgaga ttgcctcaac taccaatgaa aatatcttga cggagaaatt attggaagaa
    32581 gtggaagacg acgcaacacg ctttgctatt ctcaataact tcctagatgg tttccgtgga
    32641 acagttttcc gccaaactca atttgctgag tttgaacacg ccattcacca agcagatcaa
    32701 aatggggagg tcttgacaag cgatttccta aataaactct acgcagactt gaaccaagag
    32761 tattatggtt tgagtaagga agacaatcct gaaatccaat acgagtgggc tcgcattcca
    32821 cacttctact ataactacta tgtatatcaa tattcaactg gctttgcggc cgcctcagcc
    32881 ttggctgaaa aaattgtcca tggtagtcaa gaagaccgtg accgctatat cgactacctc
    32941 aaggcaggta agtcggacta tccacttaat gtcatgagaa aagctggtgt tgatatggag
    33001 aaggaagact acctcaacga tgcctttgca gtctttgaac gccgtttaaa tgagtttgaa
    33061 gcccttgttg aaaaattagg attggcataa aatggttgaa tcgtatagta agaatgctaa
    33121 ccataacatg cgtcgtcctg tcgtcaaaga agaaattgta gacttgatgc gtcagcgtca
    33181 aaagcaggtc acaggtttct tgaaagaatt ggaagaattt gcccgcaagg aaaatattcc
    33241 tattattccc catgaaacgg ttgcttattt ccgttttctt atggaaacca tgcagcctaa
    33301 aaatattctg gaaattggga cggctatcgg tttttcagct ctcttgatgg ctgaacatgc
    33361 gccaaatgct aagattacaa ctattgatcg taatccagaa atgattggtt ttgccaagga
    33421 aaattttgcc cagtttgaca gtcgcaagca aatcactctc ctagagggag atgcggtgga
    33481 tgtcttatct acactgacag agtcttatga tttcgtcttt atggattctg ccaagtctaa
    33541 atacatcgtc tttctgccag aaatcctcaa acatttggaa gttggtggtg tggttgtctt
    33601 ggatgatatt tttcaaggtg gtgatgttgc caaggatatt atggaagtcc gtcgtggtca
    33661 gcgaaccatt tatcgaggcc ttcaaaaatt atttgatgca accttagaca atccagaact
    33721 caccgcaaca ttagtgcctt taggagatgg tattctcatg cttcgtaaaa atgtagcaga
    33781 tgttcaactg tctgaaagcg aatgattttc agaaaaattt aagaaaaaat agtaaaatag
    33841 atagagtaac acttatctca aaggagtaga catgaagaaa aaattattgg caggtgccat
    33901 cacactatta tcagtagcaa ctttagcagc ttgttcgaaa gggtcagaag gagcagacct
    33961 tatcagcatg aaaggggatg tcatcacaga acatcaattt tatgagcaag tgaaaaacaa
    34021 cccttcagcc caacaagtct tgttaaatat gaccatccaa aaagtttttg aaaaacaata
    34081 tggctcagag cttgatgata aagaggttga tgatactatt gccgaagaaa aaaaacaata
    34141 tggcgaaaac taccaacgtg tcttgtcaca agcaggtatg actcttgaaa cacgtaaagc
    34201 tcaaattcgt acaagtaaat tagttgagtt ggcagttaag aaggtagcag aagctgaatt
    34261 gacagatgaa gcctataaga aagcctttga tgagtacact ccagatgtaa cggctcaaat
    34321 catccgtctt aataatgaag ataaggccaa agaagttctc gaaaaagcca aggcagaagg
    34381 tgctgatttt gctcaattag ccaaagataa ttcaactgat gaaaaaacaa aagaaaatgg
    34441 tggagaaatt acctttgatt ctgcttcaac agaagtacct gagcaagtca aaaaagccgc
    34501 tttcgcttta gatgtggatg gtgtttctga tgtgattaca gcaactggca cacaagccta
    34561 cagtagccaa tattacattg taaaactcac taagaaaaca gaaaaatcat ctaatattga
    34621 tgactacaaa gaaaaattaa aaactgttat cttgactcaa aaacaaaatg attcaacatt
    34681 tgttcaaagc attatcggaa aagaattgca agcagccaat atcaaggtta aggaccaagc
    34741 cttccaaaat atctttaccc aatatatcgg tggtggagat tcaagctcaa gcagtagtac
    34801 atcaaacgaa tagtccaaat caatgagtca gggaaaaaaa ctcgacttca ggaaaaaatg
    34861 aagcaaacat tcccaaaata aaacgcatag tacaaggttt gtactgcccc caaaagttag
    34921 acaattaatt tatctgaagg atttagttct gtattgcaca gggctaagtc cttttagttt
    34981 taccttaatt ctcttattgt tgtaataatc aata
//
LOCUS       DAVLXF010000007        34988 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLXF010000007 DAVLXF010000000
VERSION     DAVLXF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.007"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            143..2125
                     /locus_tag="VYO12_000354"
     CDS             143..2125
                     /locus_tag="VYO12_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1587017.1"
                     /translation="MKSSRLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSEEQKTSTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVVIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2426..7729
                     /locus_tag="VYO12_000355"
     CDS             2426..7729
                     /locus_tag="VYO12_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1587018.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPRAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRSDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(7896..10055)
                     /locus_tag="VYO12_000356"
     CDS             complement(7896..10055)
                     /locus_tag="VYO12_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1587019.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10052..10648)
                     /gene="recU"
                     /locus_tag="VYO12_000357"
     CDS             complement(10052..10648)
                     /gene="recU"
                     /locus_tag="VYO12_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1587020.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10714..11241
                     /locus_tag="VYO12_000358"
     CDS             10714..11241
                     /locus_tag="VYO12_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1587021.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGTLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11311..11640
                     /gene="gpsB"
                     /locus_tag="VYO12_000359"
     CDS             11311..11640
                     /gene="gpsB"
                     /locus_tag="VYO12_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1587022.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11661..12041
                     /gene="rnpB"
                     /locus_tag="VYO12_000360"
     ncRNA           11661..12041
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYO12_000360"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12126..13283
                     /locus_tag="VYO12_000361"
     CDS             12126..13283
                     /locus_tag="VYO12_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1587023.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLCSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13296..14690
                     /gene="mapZ"
                     /locus_tag="VYO12_000362"
     CDS             13296..14690
                     /gene="mapZ"
                     /locus_tag="VYO12_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1587024.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14766..16190
                     /gene="gndA"
                     /locus_tag="VYO12_000363"
     CDS             14766..16190
                     /gene="gndA"
                     /locus_tag="VYO12_000363"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1587025.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSHKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVYASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16202..16891
                     /locus_tag="VYO12_000364"
     CDS             16202..16891
                     /locus_tag="VYO12_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1587026.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVAYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESVTETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            16989..18005
                     /gene="cbpC"
                     /locus_tag="VYO12_000365"
     CDS             16989..18005
                     /gene="cbpC"
                     /locus_tag="VYO12_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1587027.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGQFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEPKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYFGNKWYYLRSSG
                     AMVTGWYQDGLTWYYLNAGNGDMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYNG
                     EWVQ"
     gene            18127..19005
                     /gene="mvk"
                     /locus_tag="VYO12_000366"
     CDS             18127..19005
                     /gene="mvk"
                     /locus_tag="VYO12_000366"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1587028.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFWVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            18987..19940
                     /gene="mvaD"
                     /locus_tag="VYO12_000367"
     CDS             18987..19940
                     /gene="mvaD"
                     /locus_tag="VYO12_000367"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1587029.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            19927..20934
                     /locus_tag="VYO12_000368"
     CDS             19927..20934
                     /locus_tag="VYO12_000368"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1587030.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            20918..21928
                     /locus_tag="VYO12_000369"
     CDS             20918..21928
                     /locus_tag="VYO12_000369"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1587031.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDNS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            22005..22703
                     /locus_tag="VYO12_000370"
     CDS             22005..22703
                     /locus_tag="VYO12_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1587032.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            22700..23695
                     /locus_tag="VYO12_000371"
     CDS             22700..23695
                     /locus_tag="VYO12_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1587033.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FFFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            23709..24341
                     /locus_tag="VYO12_000372"
     CDS             23709..24341
                     /locus_tag="VYO12_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1587034.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            24342..24583
                     /locus_tag="VYO12_000373"
                     /pseudo
     CDS             24342..24583
                     /locus_tag="VYO12_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            24565..24819
                     /locus_tag="VYO12_000374"
     CDS             24565..24819
                     /locus_tag="VYO12_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1587035.1"
                     /translation="MAANYLKAMQSYLKETDLPKLEQLVVTKSWWDTVDILDRVVGSL
                     VYEHSELEEIILILFENLFKPRQLYLQPQNSVLSNLRLAS"
     gene            24844..25110
                     /locus_tag="VYO12_000375"
                     /pseudo
     CDS             24844..25110
                     /locus_tag="VYO12_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            25260..25499
                     /locus_tag="VYO12_000376"
     CDS             25260..25499
                     /locus_tag="VYO12_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587036.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            25580..26401
                     /gene="cbpG"
                     /locus_tag="VYO12_000377"
     CDS             25580..26401
                     /gene="cbpG"
                     /locus_tag="VYO12_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV1587037.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            26420..27442
                     /gene="cbpF"
                     /locus_tag="VYO12_000378"
     CDS             26420..27442
                     /gene="cbpF"
                     /locus_tag="VYO12_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEV1587038.1"
                     /translation="MKLLKKMMQIALATFFFGLLATNTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLNPATGI
                     MQTGWQYLGNRWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQESSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAINTTVGGYYLNY
                     NGEWVK"
     gene            complement(27570..28918)
                     /locus_tag="VYO12_000379"
                     /pseudo
     CDS             complement(27570..28918)
                     /locus_tag="VYO12_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            29127..30896
                     /locus_tag="VYO12_000380"
     CDS             29127..30896
                     /locus_tag="VYO12_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV1587039.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     VAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            30920..32875
                     /locus_tag="VYO12_000381"
     CDS             30920..32875
                     /locus_tag="VYO12_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1587040.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESI
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            32877..33314
                     /locus_tag="VYO12_000382"
     CDS             32877..33314
                     /locus_tag="VYO12_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1587041.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            33376..34512
                     /locus_tag="VYO12_000383"
     CDS             33376..34512
                     /locus_tag="VYO12_000383"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV1587042.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT        10640 a         6230 c         7825 g        10293 t
ORIGIN      
        1 tatagttgag ggatttttaa atgatattca tattttttgc aaagatgttg tttgaaaaat
       61 aattttcaaa aattctgaaa attctgttga caactttctg aaaagagtct ataatggaga
      121 gaaagtttta aaggagaaaa tgatgaaaag ttcaagacta tttgcccttg cgggcgtgac
      181 attattggcg gcgactactt tagctgcatg ctctggatca ggttcaagca ctaaaggtga
      241 gaagacattc tcatacattt atgagacaga ccctgataac ctcaactatt tgacaactgc
      301 taaggctgcg acagcaaata ttaccagtaa cgtggttgat ggtttgctag aaaatgatcg
      361 ctacgggaac tttgtgccgt ctatggctga ggattggtct gtatccaagg atggattgac
      421 ttacacttat actatccgta aggatgcaaa atggtatact tctgaaggtg aagaatacgc
      481 ggcagtcaaa gctcaagact ttgtaacagg actaaaatat gctgctgata aaaaatcaga
      541 tgctctttac cttgttcaag aatcaatcaa agggttggat gcctatgtaa aaggggaaat
      601 caaagatttc tcacaagtag gaattaaggc tctggatgaa cagacagttc agtacacttt
      661 gaacaaacca gaaagcttct ggaattctaa gacaaccatg ggtgtgcttg cgccagttaa
      721 tgaagagttt ttgaattcaa aaggggatga ttttgccaaa gctacggatc caagtagtct
      781 cttgtataac ggtccttatt tgttgaaatc cattgtgacc aaatcttctg ttgaatttgc
      841 gaaaaatccg aactactggg ataaggacaa tgtgcatatt gacaaagtta aattgtcatt
      901 ctgggatggt caagatacca gcaaacctgc agaaaacttt aaagatggta gccttacagc
      961 agctcgtctc tatccaacaa gtgcaagttt cgcagagctt gagaagagta tgaaggacaa
     1021 tattgtctat actcaacaag actctattac gtatctagtt ggtacaaata ttgaccgtca
     1081 gtcctataaa tatacatcta agaccagtga agaacaaaaa acatctacga aaaaggctct
     1141 cttaaacaag gatttccgtc aggctattgc atttggattt gaccgtacag cctatgcctc
     1201 tcagttgaat ggacaaactg gagcaagcaa aatcttacgt aatatctttg ttccaccaac
     1261 atttgttcaa gcagatggta aaaactttgg cgatatggtc aaagagaaat tggtcactta
     1321 tggggatgaa tggaaggatg ttaatcttgc agattctcag gatggtcttt acaatccaga
     1381 aaaagccaag gctgaatttg ctaaagctaa atcagcttta caagcagaag gtgtgacttt
     1441 cccaattcat ttggatatgc cagttgacca gacagcaact acaaaagttc agcgcgtcca
     1501 atctatgaaa caatccttgg aagcaacttt aggagcggat aatgtagtca ttgatattca
     1561 acaactacaa aaagacgaag taaacaatat tacatatttt gctgaaaatg ctgctggtga
     1621 agactgggat ttatcagata atgtcggttg gggtccagac tttgccgatc catcaaccta
     1681 ccttgatatc atcaaaccat ctgtaggaga aagtactaaa acatatttag ggtttgactc
     1741 aggggaagat aatgtagctg ctaaaaaagt aggtctatat gactacgaaa aattggttac
     1801 tgaggctggt gatgagacta cagatgttgc taaacgctat gataaatacg ctgcagccca
     1861 agcttggttg acagatagtg ctttgattat tccaactaca tctcgtacag ggcgtccaat
     1921 cttgtctaag atggtaccat ttacaatacc atttgcattg tcaggaaata aaggcacaag
     1981 tgaaccagtc ttgtataaat acttggaact tcaagacaag gcagtcactg tagatgaata
     2041 ccaaaaagct caggaaaaat ggatgaaaga aaaagaagag tctaataaaa aggctcaaga
     2101 agatctcgca aaacatgtga aataactgtt gcaaaatata agaaaggatt tagtatttcc
     2161 cttgaatgct gaatcctttt ttacatttgt aaagaaagat tctaaatgta ctgaccccca
     2221 aaagttggag cctctttttg tcagaataga gaaaattttt gttaatttta cttgtttcct
     2281 attgctttct cagctattat ttgttatatt aaaagcataa ttatttttta tttatcaggg
     2341 ttaagcattg cactttcaga ggaaggagta ttttttaaaa aggaaatgta aacgcttgct
     2401 aaaaaatgaa aggatttaga agtttatgaa taaaggatta tttgaaaaac gttgtaaata
     2461 tagtattcgg aaattttcat taggtgttgc ttctgttatg attggagctg cattctttgg
     2521 gacaagtccg gttcttgcag atagcgtgca gtctggttcc acggcgaact taccagctga
     2581 tttagctact gctcttgcaa cagcaaaaga gaatgatgga cgtgattttg aagcgcctaa
     2641 ggtgggagaa gaccaaggtt ctccagaagt tacagatgga cctaagacag aagaagaact
     2701 attagcactt gaaaaagaaa aaccggctga agaaaaacca aaagaggata aacctgcagc
     2761 tgctaaacct gaaacaccta agacggtaac ccctgaatgg caaacggtag agaaaaaaga
     2821 acaaaaggga acagtcacta tccgagaaga aaaaggtgtc cgctacaacc aactatcctc
     2881 aactgctcaa aatgataacg caggaaaacc agccctgttt gaaaagaagg gcttgaccgt
     2941 tgatgccaat ggaaatgcaa ctgttgattt aaccttcaaa gatgattctg aaaagggcaa
     3001 atcacgcttt ggtgtcttcc tgaaatttaa agataccaat aataatgttt ttgtcggtta
     3061 tgacaaggat ggctggttct gggagtataa atctccaaca actagcactt ggtatagagg
     3121 tagtcgtgtt gctgctcctg aaacaggatc aacaaaccgt ctctctatca ctctcaagtc
     3181 agacggtcag ctaaatgcca gcaataacga tgtcaatctc tttgacacag tgactctacc
     3241 agctgcggtc aatgaccatc ttaaaaatga gaagaagatc cttctcaagg cgggctctta
     3301 tggcaatgac cgtacggttg tcagcgttaa aacagacaat caagaaggcg taaaagcgga
     3361 tgatactcct gcccagaaag aaacaggtcc ggttgttgac gatagcaagg tgacttatga
     3421 tacgatccag tctaaggtac ttaaggcagt aattgaccaa gccttccctc gtgtcaagga
     3481 atacagcttg aacgggcata ctttgccagg acaggtgcaa cagttcaacc aagtctttat
     3541 caataaccac cgaatcaccc ctgaagtcac ttataagaaa atcaatgaga caacagcaga
     3601 gtacttgatg aagcttcgcg atgatgctca cttaatcaat gcggaaatga cagtacgcct
     3661 gcaagttgtg gacaatcaat tgcactttga tgtgaccaag attgtcaacc acaatcaagt
     3721 cactccaggt caaaagattg atgacgaaag aaaactactt tcttctatta gtttcctcgg
     3781 caatgcttta gtctctgttt ctagtgatca aactggtgct aagtttgatg gggcaaccat
     3841 gtcaaacaat acgcatgtca gcggagatga tcatatcgat gtaaccaatc caatgaaaga
     3901 cctagccaag ggttacatgt atggatttgt ttctacagat aagcttgctg ctggtgtttg
     3961 gagtaactct caaaacagct atggtggtgg ttcgaatgac tggactcgtt tgacagctta
     4021 taaagaaaca gtcggaaatg ccaactatgt aggaatccac agctctgaat ggcaatggga
     4081 aaaagcttat aagggcattg ttttcccaga atacacgaag gaacttccaa gggctaaggt
     4141 tgttatcact gaagatgcca atgcagacaa gaaagtcgat tggcaggatg gtgccattgc
     4201 ttatcgtagc attatgaaca accctcaagg ttgggaaaaa gttaaggata tcacagctta
     4261 ccgtatcgcg atgaactttg gttctcaagc acaaaaccca ttccttatga ccttggatgg
     4321 tatcaagaaa atcaatctcc acacagatgg tcttgggcaa ggtgttctcc ttaaaggata
     4381 tggtagcgaa ggccatgact ctggtcactt gaactatgct gatattggta agcgtatcgg
     4441 tggtgtcgaa gacttcaaga ccctgattga gaaggctaag aaatatggag ctcatctagg
     4501 tatccacgtt aacgcttcag aaacttatcc tgagtctaaa tacttcaatg aaaaaattct
     4561 ccgtaagaat ccagatggaa gctatagcta tggttggaac tggctagatc aaggtatcaa
     4621 cattgatgct gcctatgacc tagctcatgg tcgtttggca cgttgggaag atttgaagaa
     4681 aaaacttggt gacggtctcg actttatcta tgtggacgtt tggggtaatg gtcaatcagg
     4741 tgataacggt gcctgggcta cccacgttct tgctaaagaa attaacaaac aaggctggcg
     4801 ctttgcgatc gagtggggcc atggtggtga gtacgactct accttccatc actgggcagc
     4861 tgacttgacc tacggtggct acaccaataa aggtatcaac agtgccatca cccgctttat
     4921 ccgtaaccac caaaaagatg cttgggtagg ggactacaga agttatggtg gtgcagccaa
     4981 ctatccactg ctaggtggct acagcatgaa agactttgaa ggctggcaag gaagaagtga
     5041 ctacaatggc tatgtgacta acctctttgc tcatgacgtc atgactaagt acttccaaca
     5101 cttcactgta agtaaatggg aaaatggtac accggtgact atgaccgata acggtagcac
     5161 ctataaatgg actccagaaa tgcgagtgga gttggtagat gctgacaata ataaagtagt
     5221 tgtaactcgt aagtcaaatg atgtcaatag cccacaatat cgcgaacgta cagtaacgct
     5281 caacggacgt gtcatccaag atggttcagc ttacttgact ccttggaact gggatgcaaa
     5341 tggtaagaaa ctttctactg agaaggaaaa gatgtactac ttcaatacgc aggccggtac
     5401 aacaacttgg acccttccaa gcgattgggc aaagagcaag gtttaccttt acaagctaac
     5461 tgaccaaggt aagacagaag agcaagaact aactgtaaaa gatggtaaaa ttaccctaga
     5521 tcttctagca aatcaaccat acgtacttta ccgttcaaaa caaaccaatc ctgaaatgtc
     5581 atggagtgaa ggcatgcaca tctatgacca aggatttaat agtggaacct tgaaacactg
     5641 gaccatttca ggcgatgctt ctaaggcaga aattgtcaag tctcaagggg caaacgatat
     5701 gcttcgtatt caaggaaaca aagaaaaagt tagtctcact cagaaattaa ctggcttgaa
     5761 accaaatacc aagtatgccg tttatgtcgg tgtcgataac cgtagtaatg ccaaggcaag
     5821 tatcactgtg aatactggtg aaaaagaagt gactacttat accaataagt ctctcgccct
     5881 caactatgta aaagcctacg cccacaatac acgtcgtaac aatgctacag ttgacgatac
     5941 aagttacttc caaaacatgt acgccttctt tacaactgga tcggacgtct caaatgttac
     6001 tctgacattg agtcgtgaag ctggtgatca agcaacttac tttgatgaaa ttcgtacctt
     6061 tgaaaacaat tcaagcatgt acggagacaa gcatgataca ggtaaaggca ccttcaagca
     6121 agactttgaa aatgttgctc agggtatctt cccatttgta gtgggtggtg ttgaaggtgt
     6181 tgaagataac cgcactcact tgtctgaaaa acacgatcca tatacacaac gtggttggaa
     6241 tggtaagaaa gtcgatgatg ttatcgaagg aaattggtca ctcaagacaa atggactagt
     6301 gagccgtcgt aacttggttt accaaactat tccgcaaaac ttccgttttg aagcaggtaa
     6361 gacctaccgt gtaacctttg aatacgaagc aggttcagac aatacctatg cttttgtagt
     6421 cggtaaggga gaattccagt caggtcgtcg tggtactcaa gcaagcaact tggaaatgca
     6481 tgaattgcca aatacttgga cagattctaa gaaagccaag aaggtaacct tccttgtgac
     6541 aggtgcagaa acaggggata cttgggtagg tatctactca actggcaatg caagtaatac
     6601 tcgtagtgat tctggtggaa atgccaactt ccgtggttat aacgacttca tgatggataa
     6661 tcttcaaatc gaagaaatta ccctaacagg taagatgttg acagaaaatg ctctgaagaa
     6721 ctacttgcca acggttgcca tgactaacta caccaaagag tctatggatg ctttgaaaga
     6781 ggcggtcttt aacctcagtc aggccgatga tgatatcagt gtggaagaag cgcgtgcaga
     6841 gattgccaag attgaagcct tgaagaatgc tttggttcag aagaaaacgg ctttggtagc
     6901 agaagacttt gcaagtctta cagctcctgc tcaggctcaa gaaggtcttg caaatgcctt
     6961 tgatggaaac ttatctagtt tatggcatac atcatggggc ggaggagatg taggcaagcc
     7021 tgcaaccatg gtcttgaaag aagcaactga aatcactgga cttcgttatg ttccacgtgg
     7081 atcaggttca aatggtaact tgcgtgatgt gaaacttgtt gtgacagatg agtctggcaa
     7141 ggagcatacc tttactgcaa ctgattggcc agataacaat aagccaaaag acattgattt
     7201 tggtaagaca attaaggcta agaaaattgt ccttacaggt actaagactt acggagatgg
     7261 tggcgataaa taccaatctg cagcggaact tatctttact cgtccacagg tagcagaaac
     7321 acctcttgac ttgtcaggct atgaagcagc tttggctaag gctcagaaat taacagacaa
     7381 agacaatcaa gaggaagtag ctagcgttca ggcaagcatg aaatatgcga cggataacca
     7441 tctcttgacg gaaagaatgg tggaatactt tgcagattat ctcaaccaat taaaagattc
     7501 tgctacgaaa ccagatgctc caactgtaga gaaacctgag tttaaactta gctctttagt
     7561 ttccaatcaa ggtaagacgc cagattataa gcaagaaata gatagaccag aaacacctga
     7621 acaaatcttg ccagcaacag gtgagagtca atctgacaca gccctcttcc tagcaagtgt
     7681 tagtctagcc ctatctgctc tctttgtagt aaaaacgaag aaagactagt atttagtaaa
     7741 acctcttaac aagattacgg aagcagtctc tatcttttcc aatgaggttt atagtacaga
     7801 aaaagcctga gaagatgtct tctcaggctt ttgttaagca cataaataca atagtgctat
     7861 gacaaaatca cccagaaaaa tctgggtgat aaatgttatg gttgtgctgg ttgaggattc
     7921 tgattttgtt gatcaggggt tgtatttgat tgttgcgtat tattgttagg attggtagtc
     7981 gtactattat ttgtgcttgg agtggttgag ctagactgtg aagttgaact atctgatgat
     8041 gagcttgaac tttcagttga tgggggttgt tgtggagcag gtgagttcca cgtagaacga
     8101 gcaccatttt taaatacgaa ttctccattt ctgtagagcc cctctggtat attccaatcc
     8161 tctggattgc ttccttcaga cagataggtc atcatagagc ggtaaacttt ggcagcgacc
     8221 gtaagaccat tgcctacaag tggtgtcaga cggttagaat agcctgtcca tacagccatt
     8281 gaatatttac gcgtatagcc agcaaatagt tcatcaggtg ctacaaattg agaggtcttg
     8341 atgtggtttt caatttcctc gtctgtatag ttagaggttc ctgttttacc agcctgaggg
     8401 agccaagcaa gataggcatt tcgtccagtt ccataactca agactgtttt catcatgtcg
     8461 gtcatcatat aggctgtcgt ttccttcatg gcacgagttc cgacattaga gaactctttt
     8521 tcactcccat cactaaagac gactttatgg atatacattg gtttatagta agttccacca
     8581 tttgcaaagg cagcgtaagc agcagccatc ttttcactac ttgctccata ttttttgtct
     8641 gattcggttg tgttacttga aatggcattt gagtagtgaa tacttgggta gtcgattccg
     8701 agaccattta ggaaagtctt ggcgcggttg agtccgacct tgtttagagt ttccacggct
     8761 gggacgtttc gcgattgttg cagggcgtat tgcaaggtga tgttgccaaa gtagccccta
     8821 tcccagttat aaacaggggt atttgtccca gggtagttat agggctcatc gtgaacgata
     8881 gtagcagttg aatcgtagat accgtactcc aaggcaggag catagtctgt gatcggtttc
     8941 atagttgatc cccagtcgcg gtttgtttct actgcttggt taattccgaa ggaaacatta
     9001 cttgactgat ggcgtgctcc tagctgggca atgactttac cgttagaaac atcaacaatg
     9061 gtagaagcga cttgcaattc atcgtctgga taggcaacgt attcgtctgt attgtaaata
     9121 tcccacagat gtttttgagc ttcttggtct acatttgtgt agacatccat cccagttgtg
     9181 agcaggttat agcctgtttc ttcttcaact tgattgatga cttccttgag gtaattatcc
     9241 atgtaagcag ggtaattact tgctgatttg agactttgta gtccatcagt aattggtgta
     9301 ttgactgctt tctcatactg ttcagcagag atgtagcctt gatttttcat ttcagataag
     9361 accaagtttc ggcggtcttg ggctgcttct ggatgtgaat aggggtcata ttggtttggt
     9421 gcctgaggca ttccagccag caaggctaac tgaggtaaac ttaaattatt gaggtcttta
     9481 ccatagtagt tttgagctgc tgtctgcatt ccatagttcc cattagacat gtagacctta
     9541 tttatatagt aggtcaagat ttcttgcttg gttgcttttt gttctaactg aatcgctaac
     9601 caagcttcct gagccttacg agaaatagtc tggtcggaag tcgaagttga aaagtaagtc
     9661 aacttaatca actgttgagt gagagttgat ccaccttgga gggaattgct ttgcagattg
     9721 cgcaagaaag ctcccaggat acggatggta tcaatccccc tgtggtcgaa gaagcgatgg
     9781 tcttcgatag aaacgattgc tttaaccaaa tctgtgggaa tatcattagc ttgggcattg
     9841 acgcggcgtt cagaacccaa gtcagcaatg agttgatttt tattgtcgta gattttacta
     9901 gaagttgttg caactagttt actctcggat aggctaggag ccttgctaac gtagtagaaa
     9961 aaaactcctc cgcctaagac aatggctgcg ataaccaagc ttaagaagct aatgctcaga
    10021 tacttgatta ggcgcagaat cgttggtttg ttcatcttgt tttaccacct aataaatgtt
    10081 ctttgataac attgagataa ggaatttgag ggaaggcacc agccttgatt tcatatccat
    10141 attctcgaat atattcaagt ggcattgatt tttgtccctt atcttgatga tagaagcgaa
    10201 tcaaatcgaa tgccggcaat aagtaggttt cttgctgaga agaaaagtga agaaggacaa
    10261 agcagattcc ttgttgggca aggacttgtt ccatatgctg aatctgatgt ggatgaaaat
    10321 ttttcatcgg aatcgcacgt ttttgttttg tttccttgac ttcaaagtcg atgtaatatc
    10381 cattataaac gccagaatag tccgtcgttg aagcttgtcg aaaataggct tcaacaatct
    10441 tggcacgact tcgttgtgga tagtccactt gtacgatttg aataggagtt ggtttcttat
    10501 gtataacagc caagccctga gacaaatagt agtcgttggt agcattgatc atcttttcaa
    10561 aagacattcc tcgatttgcg aaatttttgg gttgagaaag agatgtttgt cttttttgtg
    10621 atgaaacttt atgtggatag ttgaccataa ttctccttat tggtacaata acatcacttt
    10681 attataccat aatttgcaca gaaagggtta aaaatggcta cagctttggt tttaggctat
    10741 tctgcttttg atttgggttt attttctgat aaggatcctc gtcttaaatt gattaaaaaa
    10801 gcgatacgta aagatttaga ggctatggca gcagatgggg tgtcttggct tgtgtttaca
    10861 gggactttgg gctttgaata ttgggtttta gaggttgctc aggagatgaa aaccgaatac
    10921 ggtttccagt tggccaccat ttttgctttt gaaacccatg gggaaaattg gaatgaaggc
    10981 aatcagatga aactgagtcg ctttaagcag gtagactttg tcaaatatgc ctatcctcgc
    11041 tatgaacaca aggggcagtt aagagattac cagcagtttt tgctggaaaa cacgactagt
    11101 tcctatcttt tttatgatga agaaaatgaa acaaaactag cttattttta ccaaaagatg
    11161 aaaaatcaag aggactattt tataaagaga ttaacatttg atcaattaaa tgaacttgct
    11221 gaaaattttt ccgaaaattg aagctttgac cttgattttt gtttgccttt ttttatataa
    11281 taatactagc aagcgagaat ggagagagac atggcaagta ttattttttc agcgaaagat
    11341 atttttgaac aagagtttgg acgtgaagtc cgtggctata ataaagtaga agttgacgag
    11401 tttttagacg atgtcatcaa ggactatgaa acctatgctg ccttggtcaa gtcacttcgt
    11461 caggaaattg cggatttgaa ggaagaatta actcgtaaac cgaaaccttc accagttcaa
    11521 gcagaacccc ttgaagcggc aattacaagt tctatgacga attttgatat tttgaaacgc
    11581 ctgaatagat tggaaaaaga agtttttggt aaacaaattt tagataactc agatttttaa
    11641 gtagttattt gagatgtgca atttttggat aatcgcgtga ggagaattgc ttctcatgag
    11701 gaaagtccat gctagcacag gctgtgatgc ctgtagtgtt tgtgctaggc gaaaccataa
    11761 gcctagggac gagaaatcgt tacggcagtt gaaatggcta agtccttgga taggccagag
    11821 taggcttgaa agtgccacag tgacgaagtc tttctggaaa cagagagagt ggaacgcggt
    11881 aaacccctca agctagcaac ccaaattttg gtcggggcat ggagtacgcg gaaacgaacg
    11941 tagtattctg actgctatca gctagagctg ttagtggtag acagatgatt atcgaaggaa
    12001 gtggtcctag tcacttctgg aacaaaacat ggcttataga aaattgcata taggttgggg
    12061 ctgagaaatt ttctcaacct cattttttaa agtggacata tagaaaggtc ttgcaagact
    12121 gtaacatgaa aaaagaattt aatttaattg caactgtggc agcagggctt gaggctgtcg
    12181 ttggtcgtga agtgcgagag ttgggctacg attgtcagat tgaaaatgga cgtgttcgtt
    12241 ttcaaggaga cgtgagagct attatcgaaa ccaacctttg gctgcgggca gcagatcgta
    12301 tcaaaattat cgtaggaacg ttccctgcta agacttttga agagctattt cagggagttt
    12361 tcgctttaga ttgggaaaat tatttaccac ttggagctcg gttcccgatt tcaaaagcta
    12421 aatgtgttaa gtccaaactt cacaatgagc ccagtgttca ggctatttct aagaaagctg
    12481 ttgtcaagaa attgcagaaa cactatgctc gcccagaagg ggttcctctg atggagaatg
    12541 gcccagagtt taagattgag gtctctattc tcaaagatgt ggcaactgtc atgattgata
    12601 cgaccgggtc tagcctcttt aaacgtggtt atcgtaccga aaaaggtggc gctcctatca
    12661 aggaaaatat ggcagcagcc attttacaac tttctaactg gtatccagac aagcctttga
    12721 ttgatccgac ctgtggttcg gggactttct gtattgaggc agttatgatt gctagaaaga
    12781 tggcgccagg tcttcgtcgc tcttttgcat ttgaggaatg gaactggatc agcgatcgct
    12841 tgattcaaga agtgcgcaca gaagcggcta aaaaagtaga ccgtgagctt gagctggata
    12901 tcatgggctg tgatattgat gctcgcatgg tggaaattgc taaggccaat gctcaggcag
    12961 ctggtgttgc aggagacatt acttttaagc agatgcgcgt gcaggattta tgttccgata
    13021 aaatcaatgg agtaatcatt tccaatccgc cttatggtga acgtttgtca gatgatgcag
    13081 gggtgaccaa gctctatgct gagatggggc aagtatttgc accgctgaaa acttggagca
    13141 aatttatcct gactagtgat gaagcttttg aaagcaagta tggtagccaa gcagataaga
    13201 agcgtaagtt atacaacgga accttgaaag tggatctgta tcaatatttt ggtcagcgtg
    13261 tcaaacggca agaggtaaaa tagaaaggga tactcatgag taaaaaaaga cgaaatcgtc
    13321 aaaaaaaaga aggtcaagaa ccgcaatttg attttgatga agcaaaagag ctaacagttg
    13381 gtcaagctat tcgtaaaaat gaagaagtgg aagcaggagt cttgcctgag gattccattt
    13441 tggacaagta tgttaagcaa cacagagatg aaattgaggc ggataagttt gcgactcgtc
    13501 aatacaaaaa agaggagttc gttgaaactc agagtctgga tgatttaatt caagagatgc
    13561 gtgaggctgt agagaagtca gaagcttctt cggaggaagt tccatcttct gaagacatct
    13621 tactaccctt gcctctggac gatgaggagc aaggcttgga tcctctattg ctagatgatg
    13681 aaaatccaac agaaatgact gaagaagtgg aagaggagca aaacctttct cgtctggatc
    13741 aagaggactc agaaaagaaa agtaaaaaag gctttatttt gaccgttttg gcgcttgtat
    13801 cagtaattat ttgtgtcagt gcttattatg tctaccgtca agtggctcgt tcgaccaagg
    13861 aaattgaaac ttctcaatca actacagcca atcaatcgga tgtggatgat tttaatacac
    13921 tttatgacgc cttttacaca gatagcaata aaacggcttt gaaaaatagc cagtttgata
    13981 aactgaatca actcaagact ttacttgata agctggaagg tagtcgtgaa catacgcttg
    14041 ccaaatctaa atatgatagt ctagcaacgc aaatcaaggc tattcaagat gtcaatgctc
    14101 aatttgagaa accagctatt gtggatggtg tgttggatac caatgccaaa gccaaatcgg
    14161 atgctaaatt tacggatatt aaaactggaa atacggagct tgataaagtg ctagataagg
    14221 ctatcagtct tggtaagagc cagcaaacaa gtacttctag ctcaagttca agtcaaacta
    14281 gcagctcaag ttcaagtcaa gcaagttcaa atacgactag tgagccaaaa ccaagtagtt
    14341 caaatgagac tagaagtagt cgcagtgaag tcaatatggg tctctcgagt gcaggggttg
    14401 ctgttcaaag aagtgccagt cgtgttgcct ataatcagtc tgctattgat gatagtaata
    14461 actctgcctg ggattttgcg gatggtgtct tggaacaaat tctagcgact tcacgttcac
    14521 gtggctatat cactggagac caatatatcc ttgaacgtgt caatatcgtt aacggcaatg
    14581 gttattacaa cctctataag ccagatggaa cctatctctt tacccttaac tgtaagacag
    14641 gctactttgt cggaaatggc gctggtcatg cggatgactt ggactactaa gcagtcgtta
    14701 caaaattctt tcttttcaaa agtaaaaatg ataaaataaa acaaatgaaa caagaggagt
    14761 gtcaaatgac aaaagctaac tttggtgtcg taggtatggc cgtaatgggt cgtaaccttg
    14821 cccttaatat tgaatcacgt ggttacacag ttgctatcta caaccgtagt aaagaaaaaa
    14881 cggaagatgt gattgcttgc catcctgaaa agaattttgt accaagctat gacgttgaaa
    14941 gttttgtaaa ctcaatcgaa aaacctcgtc gtatcatgct gatggttcaa gctggacctg
    15001 gtacagatgc tactatccaa gcccttcttc cacaccttga caagggtgat atcttgattg
    15061 acggaggaaa cactttctac aaagatacca tccgtcgtaa tgaagaattg gcaaactcag
    15121 gtatcaactt tatcggtact ggagtttctg gtggtgaaaa aggtgccctt gaaggtcctt
    15181 ctatcatgcc tggtggacaa aaagaggcct acgaattggt tgcggatgtt cttgaagaaa
    15241 tctcagctaa agcaccagaa gatggcaaac catgtgtgac ttacatcggc cctgatggag
    15301 ctggtcacta tgtgaaaatg gttcacaatg gtattgagta cggtgatatg caattgatcg
    15361 cagaaagcta tgacttgatg caacacttgc taggcctttc tgcagaggat atggctgaaa
    15421 tctttactga gtggaacaag ggtgaattag acagctattt gatcgaaatc acagctgata
    15481 tcttgagcca taaagacgat gaaggccaag atggaccaat cgtagactac atccttgatg
    15541 ctgcaggtaa caagggaact ggtaaatgga ctagccaatc atctcttgac cttggtgtac
    15601 cattgtcact gattactgag tcagtgtttg cacgctacat ttcaacttac aaagaagaac
    15661 gtgtatatgc tagcaaggtg cttccaaaac cagctgcctt caactttgaa ggagacaagg
    15721 ctgaattgat tgaaaagatc cgtcaagccc tttacttctc aaaaatcatt tcatacgcac
    15781 aaggatttgc tcaattgcgt gtagcctcta aagaaaacaa ctggaacttg ccatttgcag
    15841 atatcgcatc tatctggcgt gatggctgta tcatccgttc tcgtttcttg caaaagatta
    15901 cagatgctta caaccgcgat gcagatcttg ccaaccttct tttggacgag tacttcttgg
    15961 atgttactgc taagtatcaa caagcagtgc gtgatatcgt agctcttgcg gttcaagctg
    16021 gtgtgccagt gccaactttc tcagcagcta ttacttactt tgatagctac cgttcagctg
    16081 accttccagc taacttgatc caagcacaac gtgactactt tggtgctcac acttaccaac
    16141 gtaaagacaa agaaggaacc ttccactact cttggtatga cgaaaaataa gtaggtcagc
    16201 catggggaaa cggattttat tacttgagaa agaacgaaat ctagctcatt ttttaagttt
    16261 ggaactccag aaagagcagt atcgggttga tctggtagag gaggggcaaa aagccctctc
    16321 catggctctt cagacagact atgatttgat gttattgaac gttaatctgg gagatatgat
    16381 ggctcaggat tttgcagaaa aattgagtcg aactaaacct gcctcagtca tcatgatttt
    16441 agatcattgg gaagacttgc aagaagagct ggaagttgtt cagcgttttg cagttgcata
    16501 catttataag ccagtcctta tcgaaaatct ggtagcgcgt atttcggcga tcttccgagg
    16561 tcgggacttc attgatcaac actgcagtct gatgaaagtt ccaaggactt accgcaatct
    16621 taggatagat gttgaacatc acacggttta tcgtggtgaa gagatgattg ctctgacccg
    16681 ccgtgagtat gaccttttgg cgacacttat gggaagcaag aaagtattga ctcgtgagca
    16741 attgttggaa agtgtttgga agtatgaaag tgtgaccgag acaaatatcg tagatgtcta
    16801 tatccgctat ctacggagca agcttgatgt taaaggccaa aaaagctaca ttaaaaccgt
    16861 gcgtggtgtt ggatatacca tgcaagaata gataagcagt tgcagttgtg taactgcttt
    16921 tttgaggagt ttttatatat tgacatgcag tcgggtcttt gctacaatcg ttttaggagg
    16981 atatatagat gaaacttttg aaaaaaatga tgcaagttct actagcagtc tttttctttg
    17041 gtttgctagc tacaaatacg gtatttgcga ataccacagg tggccaattt gttgataagg
    17101 ataatagaaa atattatgta aaagatgatc ataaagcaat ctattggcat aaaatagacg
    17161 gtaaaactta ctattttggt gatattggag agatggttgt cggttggcaa tacttagaaa
    17221 ttcctggaac aggttatcgt gataatttat tcgataacca accagttaat gagattggcc
    17281 ttcaggagaa gtggtactat tttggacaag atggtgcttt gctagaacaa acagataaac
    17341 aagtactaga gccaaaaacg tctgaaaata caggaaaagt atacggtgaa caatatcctc
    17401 tatctgctga aaagagaact tattattttg ataataatta tgctgtaaag acaggctgga
    17461 tttatgaaga cggcaattgg tattatttaa ataagctagg aaattttggc gatgattctt
    17521 acaatccact accaattggt gaagttgcta agggttggac tcaagatttt catgttacta
    17581 ttgacattga tagaagcaaa cctgctccat ggtactacct agatgcttca ggtaagatgc
    17641 ttacagattg gcaaaaagta aacggaaaat ggtattattt tggctcctct ggttctatgg
    17701 caacaggttg gaaatatgta cgaggcaaat ggtattactt agataataaa aatggtgata
    17761 tgaaaacagg atggcaatac tttggtaaca agtggtacta cctccgttca tcaggagcta
    17821 tggtaactgg ctggtatcaa gatggtttaa cttggtacta cctaaatgca ggtaatggag
    17881 acatgacgac aggttggttc caggtcaatg gcagatggta ctacgcttat agctcaggtg
    17941 ccttggcagt gaatacgacc gtagatggct attctgtcaa ctataatggc gaatgggttc
    18001 aataatgaaa gaagcgattg tgaatggaaa caatcgcttt ttttgtgaaa atataataaa
    18061 atagatagga aagaattaat acttgtatga aaaataagac gttttttcgt ctagtaaaag
    18121 gaaaaaatga caaaaaaagt tggtgtcggt caggcacata gtaagataat tttaataggg
    18181 gaacatgcgg tcgtttacgg ttatcctgcc atttccctgc ctcttttgga ggtggaggtg
    18241 acctgtaagg tagttcctgc agagagtcct tggcgccttt atgaggagga taccttgtcc
    18301 atggcggttt atgcctcact ggagtatttg aatatcacag aagcctgcat tcgttgtgag
    18361 attgactcgg ctatccctga gaaacggggg atgggttcgt cagcggctat cagcatagcg
    18421 gccattcgtg cagtatttga ctactatcag gctgatctgc ctcatgatgt actagaaatc
    18481 ttggtcaatc gagctgaaat gattgcccat atgaatccta gtggtttgga tgctaagacc
    18541 tgtcttagtg accaacctat tcgctttatc aagaacgtag gatttacaga acttgagatg
    18601 gatttatccg cctatttggt gattgccgat acgggtgttt atggtcatac tcgtgaagcc
    18661 atccaagtgg ttcaaaataa gggcaaggat gccctaccgt ttttgcatgc cttgggagaa
    18721 ttaacccagc aagcagaagt tgcgatttca caaaaagatg ctgaaggact gggacaaatc
    18781 ctcagtcaag cgcatttaca tttaaaagaa attggagtca gtagccctga ggcagacttt
    18841 tgggttgaaa cgactcttag ccatggtgct ctgggtgcca agatgagcgg tggtgggcta
    18901 ggaggttgta tcatagcctt ggtaaccaat ttgacacacg cacaagaact agcagaaaga
    18961 ttagaagaga aaggagctgt tcagacatgg atagagagcc tgtaacagta cgttcctacg
    19021 caaatattgc tattatcaaa tattggggaa agaaaaaaga aaaagagatg gtgcctgcta
    19081 ctagcagtat ttctctaact ttggaaaata tgtatacaga gacgaccttg tcgcctttac
    19141 cagccaatgt aacagctgac gaattttaca tcaatggtca gctacaaaat gaggtcgagc
    19201 atgccaagat gagtaagatt attgaccgtt atcgtccagc tggtgagggc tttgtccgta
    19261 tcgatactca aaacaatatg cctacggcag cgggtctgtc ctcaagttct agtggtttgt
    19321 ccgccctggt caaggcttgt aatgcttatt tcaagcttgg attggataga agtcagttgg
    19381 cacaggaagc caaatttgcc tcaggttctt cttctcggag tttttatgga ccactaggag
    19441 cctgggataa ggatagtgga gaaatttacc ctgtagagac agacttgaaa ctagctatga
    19501 ttatgttggt gctagaggac aagaaaaaac caatctctag ccgtgacggg atgaaacttt
    19561 gtgtggaaac ctcgacgact tttgacgact gggttcgtca gtctgagaag gactatcagg
    19621 atatgctgat ttatctcaag gaaaatgatt ttgccaagat tggagaatta acggagaaaa
    19681 atgccctggc tatgcatgct acgacaaaga ctgctagtcc agccttttct tatctgacgg
    19741 atgcctctta tgaggctatg gactttgttc gccagcttcg tgagaaagga gaggcctgct
    19801 actttaccat ggatgctggt cccaatgtta aggtcttctg tcaggagaaa gacttggagc
    19861 atttatcaga aattttcggt catcgttatc gcttgattgt gtcaaaaaca aaggatttga
    19921 gtcaagatga ttgctgttaa aacttgcgga aaactctatt gggcaggtga atatgctatt
    19981 ttagagccag ggcagttagc tttgataaag gatattccca tctatatgag ggctgagatt
    20041 gctttttctg acagctaccg tatctattca gatatgtttg atttcgcagt ggacttaagg
    20101 cctaatcctg actacagctt gattcaagaa acgattgctt tgatgggaga cttcctcgct
    20161 gttcgcggtc agaatttaag acctttttcc ctaaaaatct gtggcaaaat ggaacgagaa
    20221 gggaaaaagt ttggtctagg ttctagtggc agcgtcgttg tcttggttgt caaggcttta
    20281 ttggctctct ataatctttc ggttgatcag gagctcttgt tcaagctgac tagcgctgtc
    20341 ttgcttaagc gaggagacaa tggttccatg ggcgaccttg cctgtattgt ggcagaggat
    20401 ttggttctct accagtcatt tgatcgccag aaggcggctg cttggttaga agaagaaaac
    20461 ttggcgacag ttctggagcg tgattgggga ttttttatct cacaagtgaa accaacttta
    20521 gaatgtgatt tcttagtggg atggaccaag gaagtggctg tatcgagtca catggtccag
    20581 caaatcaagc aaaatatcaa tcaaaatttt ttaagttcct caaaagaaac ggtggtttct
    20641 ttggtcgaag ccttggagca ggggaaagcc gaaaaagtta tcgagcaagt agaagtagcc
    20701 agcaagcttt tagaaggctt gagtacagat atttacacgc ctttgcttag acagttgaaa
    20761 gaagccagtc aagatttgca ggccgttgcc aagagtagtg gtgctggtgg tggtgactgt
    20821 ggcatcgccc tgagttttga tgcgcaatca accaaaacct taaaaaatcg ttgggccgat
    20881 ctggggattg agctcttata tcaagaaagg ataggacatg acgacaaatc gtaaggacga
    20941 gcatatcctc tatgcccttg agcagaaaag ttcctataat agctttgatg aggtggagct
    21001 gattcattct tccttgcctc tttacaatct ggatgaaatc gatctttcga cagagtttgc
    21061 tggtcgaaag tgggactttc ctttttatat caatgccatg actggtggaa gtaataaggg
    21121 aagagaaatc aatcaaaagc tggctcaggt ggcggaatcc tgtggtattt tatttgtaac
    21181 gggttcttat agcgcagccc tcaaaaatcc aacggataat tctttttctg tcaagtctag
    21241 tcatcccaat ctcctccttg gaaccaatat tggattggac aaacctgtcg agttaggact
    21301 tcagactgta gaagagatga atcctgttct attgcaagtg catgtcaatg tcatgcagga
    21361 attactcatg cccgagggag aaaggaagtt tagaagctgg caatcgcatc tagcagatta
    21421 tagcaagcaa attcccgttc ctattgtcct caaggaagtg ggctttggaa tggatgccaa
    21481 gacaatcgaa agagcctatg aattcggtgt tcgtacagtg gacctatcgg gtcgtggtgg
    21541 caccagcttt gcctatatcg aaaaccgtcg tagtggccag cgtgattacc tcaatcaatg
    21601 gggtcagtct accatgcagg cccttctcaa tgcccaagaa tggaaagata aggtcgaact
    21661 cttggttagt ggaggggttc ggaatccgct ggatatgatt aagtgcttgg tttttggtgc
    21721 taagtctgtg ggattgtcac gaaccgttct ggaattggtt gaaacctaca cagttgaaga
    21781 agtgattggc attgtccaag gctggaaagc agacctacgc ttgattatgt gttcccttaa
    21841 ctgtgccacc atagcagatc tacaaaaagt agactatctt ctttatggaa aattaaaaga
    21901 agcaaatgat cagatgaaaa aggcgtaacc agcgcctttt ttccatcctc agactgaggt
    21961 gacttttttg aattgtgata aaatagaagg gagaggatga acctatgaga aaatttaaaa
    22021 tctttttatt tatcgaagcc tgtcttctga caggagctct gattttgatg gtatcagagc
    22081 atttttcgcg ttttctgctg atactattcc tctttttgct tttgattcgc tactacactg
    22141 gtaaagaggg gaataatctt cttttagtag cggcaaccat tctcttcttt ttcattgtta
    22201 tgctcaatcc ttttgtgatt ctagctattt ttgttgcggt tatctatagt ctctttcttc
    22261 tttacccgat gatgaaccag gaaaaagagc agaccaattt ggtttttgaa gaggttgtga
    22321 cggttaagaa ggagaaaaat cgttggtttg gaaatcttca tcatttttca agctaccaga
    22381 cttgccaatt tgatgatatc aatctctttc gcttcatggg caaggacact attcatctgg
    22441 agagagtcat cttaaccaat catgacaatg tcattatcct cagaaagatg gtaggaacga
    22501 ccaaaatcat cgtacctgta gatgtggaag tcagtctcag cgttaactgt ctctatgggg
    22561 atttgacttt tttcaaccag cccaagcgag ccctccgcaa tgaacactat catcaagaaa
    22621 caaaagacta tctcaagagt aacaagagtg tcaagatttt cttgaccact atgattggtg
    22681 atgttgaggt ggttagagga tgaaaaaaca agcctatgtc attatcgctc tcacctcctt
    22741 cctgtttgtc ttttttttct cccatagctt gctggaaata cttgattttg actggtctat
    22801 ctttttgcac gatgtcgaaa aaacagaaaa atttttcttt ttattgttgg ttttcagcat
    22861 gtccatgacc tgtctcttag ccctgttttg gcgagggatc gaagagcttt ctctaagaaa
    22921 aatgcaggct aatctcaagc gtttattggc agggcaagaa gtggttcagg ttgtagatcc
    22981 agatttggat gccagtttca agtccttatc aggtaaactt aaccttttga cagaggctct
    23041 tcaaaaagct gaaaatcata gccttgctca ggaagaggaa atcatcgaga aggaacggaa
    23101 gcgaattgct cgggatttgc acgatacagt cagtcaggag ttgtttgcgg cccacatgat
    23161 tttatcgggt atcagtcagc aggctttgaa attggataga gaaaagatgc agacccagtt
    23221 gcagagtgtc acagctattt tagaaacagc ccagaaggat ttgcgggttt tgctcttgca
    23281 tttgcgacca gttgaactgg agcagaagag cttgatagaa gggattcaaa ttcttttaaa
    23341 agagcttgag gacaagagtg atcttagggt tagtctcaag cagaatatga cgaaattgcc
    23401 taagaaaatc gaggagcata tcttccgtat cctgcaagag ttgattagca ataccctccg
    23461 ccatgcccag gcatcttgcc tagatgtcta cctctatcag acagatgttg aattgcaact
    23521 gaaggtggtg gacaatggga ttggtttcca gttagggagc ttagacgact tgagttatgg
    23581 actgcgaaat atcaaggagc gggttgaaga tatggctgga acagttcaac tcttgacagc
    23641 tcccaagcaa gggctggcgg ttgatatccg tattcctctg ttagataagg aatgataaag
    23701 gagtaaagat gaaaatttta ctagtagatg accatgaaat ggtccgattg ggcttgaaaa
    23761 gctactttga cctccaagac gatgtagaag ttgtgggtga ggcgtccaac gggtctcaag
    23821 ggattgactt ggccttggaa ctgcgtccag atgtcattgt catggatatt gtcatgcctg
    23881 agatgaatgg gattgacgcg accttagcaa tccttaaaga atggcctgaa gccaagattt
    23941 tgattgtgac ctcttatttg gacaatgaaa aaatcatgcc agtcttagat gctggtgcca
    24001 aaggctatat gctcaagact tctagtgcag atgaattgct tcatgccgtc agtaaggtag
    24061 ctgctggcga gctggccatt gagcaagagg ttagcaagaa ggttgaatac caccgcaatc
    24121 atatggaact tcatgaagaa ttgactgcgc gtgagcgaga tgttctccaa ctcatcgcca
    24181 agggctacga aaatcagcgc atcgcagatg acctctttat ctctctcaag acggtcaaga
    24241 cccacgtgtc caatattctt gccaaacttg aagtcagcga tcgtacccag gcggctgtct
    24301 atgcctttca gcaccatttg gtggggcaag aggagtttta gatgagttta gcagatttac
    24361 ttgaggagct agaagcagca aaagactcta aaaaagcaag gtccatggaa gcctatatgc
    24421 gccatcaatt ttcttttcta ggtattgcgg tgcctgaaag aaataaactc tataaaatat
    24481 tttccagaag cgaaaaaaac aaagattatc gattgggatt ttgcagacac ttgctgggaa
    24541 aagaatccta gaaaatacca atatgtggct gctaactatt tgaaagcaat gcagtcttat
    24601 ctaaaggaaa ccgatttgcc taagctagag cagttagtcg tgacaaaatc ctggtgggac
    24661 acggtagata tcctagatcg agtagtaggg agtttggtgt atgaacactc tgaactagaa
    24721 gaaataatct taatactctt cgaaaatctc ttcaaaccac gtcagctcta tctgcaacct
    24781 caaaacagtg ttttgagcaa tctgcggcta gcttcctagt ttgctctttg attttcattg
    24841 agtataaaat ggagcctatc agataatatc tggctgagac gtgtcgctat tgaccaccag
    24901 ttgttaagaa aagagaagac caatactcaa ttaatggaaa agattctact tcataacttg
    24961 aaccaaacag aattttttat caataaagcc attggctgga ctctgcgaga ctactccaaa
    25021 acgaatccca cttgggtaac atgctttatt gagaaaaaca aggaaagaat ggctgaactt
    25081 agtatcaaag aagcaagcaa gtacctctag cgtgattgaa aagcgcagtc attaattgaa
    25141 aagtttacct gtttatgtga taatggaggg tatttaaaga aggtggtaga tttcttgatt
    25201 ctatagtaga ttaagaaatt tagaatagta cgctataatt acattaggag gatacaataa
    25261 tgaagaaaac tgtttataaa aaattgggta tttcaattat tgcgagtact ttattggcta
    25321 gccagttatc gacagtatct gctttgagtg ttatttctag tacaggtgaa gaatatgagg
    25381 taagtgagac actagaaaaa ggtccagagt ctaatgattc ttcattatct gagatttcac
    25441 caacgtatgg ttcatactac caaaagcaat cagaagtatt atcggtaatg atgatttgat
    25501 taaaattgat aatactttac agtatccgta ttcgacttca gctatggttt tatctaagta
    25561 ttatggagta gccgatggaa tgaatgtaga agggagggga agtgcgaatt ttattaaaga
    25621 taatgtgtta attacagcgg ctcacaacta ctacagacat gactatggga aagaagcgga
    25681 tgatatttat gttcttccgg ctgttagtcc aagtcaagaa ctatttggaa agatcaaagt
    25741 aaaggaagtt cgttatttga aggaatttag aaatttaaat tctaaggatg caagggaata
    25801 tgacttggct ttattaattc tagaagagcc cattggtgca aaattaggga ctttgggtct
    25861 tcctactagt caaaaaaatt tgacaggaat aactgtgact atcacaggct atccatcata
    25921 taattttaaa attcatcaaa tgtatacaga taaaaaacaa gttttaagtg atgatggcat
    25981 gttcttggat taccaagttg atactttaga ggggtctagt ggatctacag tttatgatgc
    26041 tagtcaccgt gtagtaggag tgcatacttt aggagatgga gctaatcaaa ttaacagtgc
    26101 agttaaatta aatgaacgaa atttgccatt tatttattcg gttcttaaag gttactctct
    26161 tgaaggatgg aagaaaataa atggtagttg gtaccattat agacaacatg ataaacaaac
    26221 gggttggcag gagataaatg atacttggta ttatttagac agttccggta agatgcttac
    26281 agattggcaa aaagtaaatg gaaaatggta ttatctcaat tcaaatggag caatggttac
    26341 aggtagccaa actatcgatg gtaaagttta taacttcgct tcatctggtg agtggattta
    26401 atgttggagg atatataaaa tgaagctttt gaaaaaaatg atgcaaatcg cactagccac
    26461 atttttcttc ggtttgttag cgacaaatac agtatttgca gatgattctg aaggatggca
    26521 gtttgtccaa gaaaatggta gaacctacta caaaaagggg gatctaaaag aaacctactg
    26581 gagagtgata gatgggaagt actattattt tgatccttta tccggagaga tggttgtcgg
    26641 ctggcaatat atacctgctc cacacaaggg ggttacgatt ggtccttctc caagaataga
    26701 gattgctctt agaccagatt ggttttattt tggtcaagat ggtgtattac aagaatttgt
    26761 tggcaagcaa gttttagaag caaaaactgc tacgaatacc aacaaacatc atggggaaga
    26821 atatgatagc caagcagaga aacgagtcta ttattttgaa gatcagcgta gttatcatac
    26881 tttaaaaact ggttggattt atgaagaggg tcattggtat tatttacaga aggatggtgg
    26941 ctttgattcg cgcatcaaca gattgacggt tggagagcta gcacgtggtt gggttaagga
    27001 ttaccctctt acgtatgatg aagagaagct aaaagcagct ccatggtact atctaaatcc
    27061 agcaactggc attatgcaaa caggttggca atatctaggt aatagatggt actacctcca
    27121 ttcgtcagga gctatggcaa ctggctggta taaggaaggc tcaacttggt actatctaga
    27181 tgctgaaaat ggtgatatga gaactggctg gcaaaacctt gggaacaaat ggtactatct
    27241 ccgttcatca ggagctatgg caactggttg gtatcaggaa agttcgactt ggtactatct
    27301 aaatgcaagt aatggagata tgaaaacagg ctggttccaa gtcaatggta actggtacta
    27361 tgcctatgat tcaggtgctt tagctattaa taccacagta ggtggttact atttaaacta
    27421 taatggtgaa tgggttaagt aatgaagact aattgtaaac tgtgatggat acttaacttt
    27481 gtataatagg tggataaaag tcttcacaat caaaaaacgc atagtatcaa ggtttttctg
    27541 tactgccccc caaaagttag acaaataatt tatccgaagg atttagttct gtattgcaca
    27601 gggctaagtc cttttagttt taccttaatt cgtttattgt tgtagtaatc aatatagtct
    27661 acaatagctt gttccaattg attaagtgat ttaaatgttt tctcatagcc ataaaacatt
    27721 tccgatttca gaatcccaaa gaaggactcc atcataccgt tgtcttggct gtttcccttg
    27781 cgtgacatgg atgcttgaat tcccttaccc tcaagaaact gatgataaga atcgtgttga
    27841 tattgccagc cttggtcact atggagaatc gtattctcgt agtgcttctc tgtgaatgcc
    27901 tgttccaaca ttgtttgtac ttgttctaag ttgggtgaag ttgaaagatt ataggcgata
    27961 atttcgctat taaagccatc taaaactggt gataagtaaa gcttttgagt acttgctgga
    28021 atggcaaatt ctgtcacatc tgtgtagcac ttttccattg ttttagagcc ttcaaattgg
    28081 ccttgaatga gattctctgc cttcttgcca acgtctcctt tatgagaaga atattttcgt
    28141 ttctgtcgca ttttagcttg taaattgagt acttttatca agccttgaac tcttttatga
    28201 tttaccagat aaccacgatt tcttagttct aaataaattc gacgataagc ataatttccc
    28261 ttgtgttcga taaaaatgga ttgaatttca gctttaagct cttggtcctt atctgttttg
    28321 tctagctgtt tcaagtgata gtagtaggtc gaacgagcta gtttaatgga ttttagaaga
    28381 agatctaacg aaaactcagt cattaattct tgaacaattt ctgtctttct tctttctctt
    28441 tttcctcctt caatcggagt tctcttaact tttttaggat ggcattctcc gctctcaggt
    28501 actcattttc tgcttgaaga cgttctaatt ctgtcctctc ttcaggtctc gtttttggat
    28561 tacgtcccat tttaggtact ctccctcttg ttttctcaac aatagtatac ccgtttttcc
    28621 tgtattgtgc tagccagtta agaagtatcg tacgacttgg gaggccgtat tcaagagaaa
    28681 ctctatcttt agtccagcct tcatgtcaga ctttatgaat catttcttgt tttaaatcag
    28741 gagaatagta acgatttttt ccttttttga cgaactctat tccgtaacga tcaatcaatt
    28801 taatcatgta cctaagatta gaattgttta tcccaaattt atttgaaagc ttctctaagc
    28861 tatatccttg ttttctaagt tcatagatct gaactttatc atcataagtt aatttcataa
    28921 taaaaacacc ccaaaagtta gattttttct gtctaacttt tggggtgtag ttcatgtaca
    28981 cctgatatga tgcgttttat aatttttaag cctttttgcc cagcctcgtc aaaagtaatg
    29041 ttttgacaca aaatctgtga caaaacttta gttttaaagg tttttaactt tgtatatact
    29101 agttttaaga aaaggaggat gatctaatgg aagaaaaagt atcattgaag gtcagggttc
    29161 aaaaactagg gacatcgctt tcaaatatgg ttatgcccaa tattggagca tttattgctt
    29221 ggggagtatt gactgccctc tttatcgctg atggctatct gccaaatgaa cagttagcta
    29281 ctgttgttgg tcctatgtta acgtatttat tgccaatcct gattggttac acaggtggat
    29341 atatgatcca tggccaacgt ggtgccgttg taggagctat tgctactgtt ggtgcaatca
    29401 caggttctag tgttcctatg tttatcggag ctatggtaat gggcccactg ggaggatgga
    29461 ctatcaagaa atttgatgag aagttccagg aaaaaattcg tcccggattt gaaatgttag
    29521 ttaataactt ctcagctggt ctcgttggtt ttgcattatt gcttttggct ttctacgcaa
    29581 tcggtccagt cgtatcgact cttactgtag ctgttgggaa tggtgttgag gctattgtca
    29641 atgctcgcct ccttcctatg gctaatatta tcatcgaacc ggctaaagtc ctttttctca
    29701 ataatgccct caatcatggc atttttactc ctctgggagt agaacaggta gctcaagctg
    29761 gtaagtcaat tctcttccta ttggaagcta atcctggacc aggcctggga attctattag
    29821 cttatgctgt attcggtaaa ggttctgcta aatcttcttc ttggggggca atggttattc
    29881 atttcttcgg agggattcat gaaatttact ttccttatgt tatgatgaag cctactctat
    29941 ttttagctgc tatggcagga ggtatctctg gaacttttac ttttcaactc ttagacgctg
    30001 gtcttaaatc tccagcttca ccaggttcta ttattgcgat tatggctacg gcgccaaaag
    30061 gtgtttggcc tcatctaaat gttcttttag gtgttttagt ggcagcagtt gtttctttcc
    30121 ttgtagcagc ccttattctt catgcagaca agtcaactga ggattcgctc gaagctgctc
    30181 aggcggctac ccaagcagct aaggctcagt ctaaaggtca gttagtatca acttctgttg
    30241 atgcagttgt ttcgacagac tcagtggaaa aaatcatttt cgcctgcgat gctggtatgg
    30301 gaagctctgc tatgggagct agtattcttc gagataaggt taaaaaagca ggtctagaga
    30361 ttccagtatc taatcaggca atctcaaatt tgcttgatac accaaaaaca ttaattgtta
    30421 ctcaggaaga actgacacca agagctaaag acaagagtcc aagtgctatt catgtttctg
    30481 ttgataattt cttagcgtcc cctcgttatg atgaaattgt agcttcatta acaggagctt
    30541 ttccaatagc agaaattgaa ggagatatac caacttcagc accagtagat agtcaggaaa
    30601 ttgaccttaa ccatattgat gctgtagtag ttgcttatgg taaagcacag ggaactgcaa
    30661 ctatgggctg tgaaacgatt cgggctatct ttagaaacaa gaatattcgt attccagttt
    30721 ctactgccaa aatttcagaa ttaggtgaat ttaattctaa aaacataatg attgtaacaa
    30781 ctatttcttt acaggcagaa gtgcagcaag cagcaccgaa ttctcaattt cttattgtgg
    30841 atagtttagt aacaacacca gaatatgaca aaatggctgc tagaatgtac aaatagaact
    30901 agaggtttct aaattacgaa tgctattaac caaacgagaa gaacaattat tgaaggcttt
    30961 cctacatgta gggaagcttt caatgcaaga tatgactgaa atcttacagg tttcatctag
    31021 aacaatttat cgaactttat cagatttgac agatagcatg gagcaatatg gaatcgaaat
    31081 aacgaagcat gggaaatact atattttgac tggagagttg gatgatttgc cgacagaact
    31141 tgaagtgtta gttgagtata gtccccaaga aagacaagag ttgattacct atcgccttct
    31201 gactgagagc ggttttgtca ccaatgaagc attgcaagag tgcacgaaag tcagtaatgt
    31261 aactattatt caggatattt cagatattga taagcgtctt ttagactttg atctgaaaat
    31321 tgaacgacaa aaaggttatc ggatttctgg tgattcagtt ggtaagagaa gatttttggc
    31381 tattttactg acaaactgta tctcagtagc agatttttca accggtaatt ttgggagctt
    31441 tgatatttta gaagcagata gaactaggct ggccagtcag attgttaata agcaactgtc
    31501 aggttttcca gatatggatg ctaggatgaa gatgtttttt gcgatcttgt tatctcttat
    31561 aggtcaggag caaaacattg aaaattcacc taatactagt aagcaggctt tggaaatttc
    31621 tcaaaaaatt tttcaagctt actctaagca gactgcacaa ttttatagta ttcaggaaat
    31681 tatctatttt gcgagcatct tggatgaatt aatcattaaa cgtcaggaca atccgctctt
    31741 tacggagaaa tttgatggtg aatttttcta caatatttca aatctgattg atacggtttc
    31801 catgtatacc aagattgact tttttaagga caaggtttta ttcaattttc ttttccatca
    31861 tattcggctc agtttaggcg tccctatcct ttttcagggt gaaaatttgc cagaatctat
    31921 ccagatttta gttgaaagga ataaatttct ttatacagtc atcagtcttt tagtgaatga
    31981 tatttttccg aaatatcttc atacagagta tgagtatggc atgattgccc tacattttat
    32041 ctctagctta ggccgtagtc cagagattta tccagtccgt gttttgcttt taacggatga
    32101 acgtcgggtc actagagatt tattagtcag taaaattaag agtgttgctc cttttgtaga
    32161 gttgatagat attcagtctc tagtagatta ccacagtatt gatctcagtc agtatgatta
    32221 tattttatct accaagccgc tgactaatca ggaaatcgat gtaatttcta gttttccaac
    32281 cgtcaaagaa ttgcttgaat tacaggaacg acttcagtat gtacaggcac atcgtacaat
    32341 tgtcgcgcgt gatgctatcg ctccagagaa aagttatgac ttgcaagatt atttaatatc
    32401 tagtagtcag cttttgagtc aatttgagtt ggttcaattg gagaataatc aatcatttga
    32461 gcacacggta gaacaaatca tccaatatca gaagaatgtg agtgacagag attacctaac
    32521 aagaaaattg ttatctcact tccagaatag tcctatggct attcctaata ctggtctggt
    32581 gcttttacat agtcagtcta gcaaagtaac aacaaatagt tttactatgt ttgaactcaa
    32641 actacctatc tccgcattgt caatgaaacg agaggaagaa gaggtcaaaa ggtgtctgct
    32701 aatgctaatg tctaaagaag ctagcgagga agcgagagat ttaatgacag ctattagtca
    32761 gtcgattatt gaaaatcatc tttatacaga gatttacaag acgggaaatc aatccattat
    32821 ttatcagatg ctaaatacta tttttaacga aaaaattaag aaattggaga actaatatga
    32881 aacttgaaaa acatttgatt aagcttaata aacaattttc taacaaggag gaagctattt
    32941 gttattgtgg gcaagttctt tatgagggtg gatatgttaa tgaagactat attgaagcca
    33001 tgattgagcg agataaagag ctatctgttt acatgggtaa ctttatcgcc ataccgcatg
    33061 gaacagatgc agcaaaaaag gatgtactca agtctggtat tacagtcgtt caagtcccta
    33121 gaggggttga ttttgggaat gtatctaacc ctcaagtggc aacggttctt tttggtattg
    33181 ctggtattgg taatgaacac ttggaaatta ttcagaaaat ttctatcttc tgtgcagatg
    33241 tagataatgt tcttaaacta gcagatgctc agtcaaaaga ggaagtattg cgcttatttg
    33301 atgctgttga ataattgaat ttcgtcattt gtcatctagt atatatgtcc ctcaaatagg
    33361 aaaaggagaa attgaatgaa acattctgtt cattttggtg ccggtaatat cggtcgtggt
    33421 tttataggtg aaattctatt taaaaatggt ttccatattg attttgtgga tgtcaataat
    33481 cagataattc atgctctaaa tgaaaagggc aagtatgaaa ttgaaattgc acagaaagga
    33541 cagtctcgta tagaagtaac taatgtggct ggcattaata gcaaagaaca tcctgagcaa
    33601 gtcattgaag cgattcaaaa gacggatatt attactactg caatcggacc taatatactc
    33661 ccttttatcg ccgaacttct agccaaagga atcgaagctc gccgagttgc aggaaataca
    33721 caggcattgg atgttatggc ctgtgaaaat atgattggcg ggtctcaatt tctttatcaa
    33781 gaagtcaaga aatatttaag tccggaaggt ttgacatttg ctgataacta cataggtttt
    33841 ccaaatgctg cagtagacag gattgttcca gcacaaagtc acgaagattc cctttttgtt
    33901 gtggtcgagc cctttaatga atgggtcgtg gaaaccaagc gtcttaaaaa tccagattta
    33961 cgtctaaaag atgtgcatta tgaagaagat ttagaaccct ttattgagcg aaaacttttt
    34021 tcagtcaatt ctggacatgc aacttcagct tacattggtg cgcattatgg tgccaagaca
    34081 attttggaag ctcttcaaaa tcctaatatt aaatctcgga ttgaatctgt attagctgaa
    34141 attcggagtc tcttgattgc caaatggaac tttgataaaa aagaattgga gaattatcac
    34201 aaagtcatta tagaacggtt tgaaaaccct ttcatagtgg acgaggttag tcgcgtagct
    34261 cgtactccaa tccgaaaatt aggctataat gaacgattca tccggccgat acgtgaattg
    34321 aaagaactca gtttgtcata taaaaaccta cttaaaacag ttggctatgc ctttgactat
    34381 cgcgatgtaa atgatgaaga aagtattcga ttaggtgaat tgttggctaa acaatcagtc
    34441 aaagatgttg ttatacaagt tacaggttta gacgaccaag aattgattga gcaaattgta
    34501 gagtatattt aatctttttc gaaaatctct tcaaatcagg ttagcatcgc tttgtcttag
    34561 gcatatgttg ttctatctac aacctcaaag cagtgctttg agctgactcc gtcagtcttg
    34621 tctgcaacct caaaacactg tttgagttat ctgcggtaat ctttctagct tgtctttgat
    34681 ttttgttgtt atttataagg taaaagaagc tggacaaaaa gtcttcaaaa tcggaaaaag
    34741 gcagcctatc gggtgttcaa aaatcttgat aggatgtcct ttattatgga aagccttatt
    34801 ggattttctc ctcagattga gtttttgatc agctttatga gataggtttt gctagagatg
    34861 tagcccatca tgttattttt atggacggtg ggaaaattgt tgaaaaaaat aatgcccatc
    34921 aattctttag tcgtccaaga gaagaacgaa ccaagcaatt ttggaacgaa ttctttcgaa
    34981 tgcgatct
//
LOCUS       DAVLXF010000008        34616 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLXF010000008 DAVLXF010000000
VERSION     DAVLXF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.008"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            573..1025
                     /locus_tag="VYO12_000384"
                     /pseudo
     CDS             573..1025
                     /locus_tag="VYO12_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            1044..2381
                     /locus_tag="VYO12_000385"
     CDS             1044..2381
                     /locus_tag="VYO12_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1587043.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2460..3344
                     /locus_tag="VYO12_000386"
     CDS             2460..3344
                     /locus_tag="VYO12_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1587044.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3344..4177
                     /locus_tag="VYO12_000387"
     CDS             3344..4177
                     /locus_tag="VYO12_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1587045.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLITIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4196..6289
                     /gene="nanB"
                     /locus_tag="VYO12_000388"
     CDS             4196..6289
                     /gene="nanB"
                     /locus_tag="VYO12_000388"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1587046.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6302..7405
                     /locus_tag="VYO12_000389"
     CDS             6302..7405
                     /locus_tag="VYO12_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1587047.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7579..8277
                     /locus_tag="VYO12_000390"
     CDS             7579..8277
                     /locus_tag="VYO12_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1587048.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8296..9813
                     /locus_tag="VYO12_000391"
     CDS             8296..9813
                     /locus_tag="VYO12_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1587049.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9938..11266
                     /locus_tag="VYO12_000392"
     CDS             9938..11266
                     /locus_tag="VYO12_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1587050.1"
                     /translation="MKFRKLACTVLAGAVVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11518..12384
                     /locus_tag="VYO12_000393"
     CDS             11518..12384
                     /locus_tag="VYO12_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1587051.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12400..13239
                     /locus_tag="VYO12_000394"
     CDS             12400..13239
                     /locus_tag="VYO12_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1587052.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13512..13964
                     /locus_tag="VYO12_000395"
     CDS             13512..13964
                     /locus_tag="VYO12_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1587053.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCYEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14097..14405
                     /locus_tag="VYO12_000396"
     CDS             14097..14405
                     /locus_tag="VYO12_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587054.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGAYAAKYGISMARSI
                     LSRAAATAAARVGLSTKISGWILRVAVNVADVYGNFAYNIAAAWDAHDKIPNNGRINF
                     "
     gene            14689..15333
                     /locus_tag="VYO12_000397"
     CDS             14689..15333
                     /locus_tag="VYO12_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1587055.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15502..16419
                     /locus_tag="VYO12_000398"
     CDS             15502..16419
                     /locus_tag="VYO12_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1587056.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16437..17321
                     /locus_tag="VYO12_000399"
     CDS             16437..17321
                     /locus_tag="VYO12_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1587057.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGLVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASMTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17337..18188)
                     /locus_tag="VYO12_000400"
     CDS             complement(17337..18188)
                     /locus_tag="VYO12_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1587058.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18388..20445
                     /gene="pbp2b"
                     /locus_tag="VYO12_000401"
     CDS             18388..20445
                     /gene="pbp2b"
                     /locus_tag="VYO12_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1587059.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSELTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20456..21052
                     /gene="recR"
                     /locus_tag="VYO12_000402"
     CDS             20456..21052
                     /gene="recR"
                     /locus_tag="VYO12_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1587060.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21228..22271
                     /locus_tag="VYO12_000403"
     CDS             21228..22271
                     /locus_tag="VYO12_000403"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1587061.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALELAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22355..23728
                     /locus_tag="VYO12_000404"
     CDS             22355..23728
                     /locus_tag="VYO12_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1587062.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            23715..24326
                     /locus_tag="VYO12_000405"
     CDS             23715..24326
                     /locus_tag="VYO12_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1587063.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGQREQYLD"
     gene            24417..25121
                     /locus_tag="VYO12_000406"
     CDS             24417..25121
                     /locus_tag="VYO12_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1587064.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25340..26713
                     /gene="ftsA"
                     /locus_tag="VYO12_000407"
     CDS             25340..26713
                     /gene="ftsA"
                     /locus_tag="VYO12_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1587065.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRTGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26730..27989
                     /gene="ftsZ"
                     /locus_tag="VYO12_000408"
     CDS             26730..27989
                     /gene="ftsZ"
                     /locus_tag="VYO12_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1587066.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27994..28665
                     /locus_tag="VYO12_000409"
     CDS             27994..28665
                     /locus_tag="VYO12_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1587067.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28675..29214
                     /locus_tag="VYO12_000410"
     CDS             28675..29214
                     /locus_tag="VYO12_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1587068.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29214..29477
                     /locus_tag="VYO12_000411"
     CDS             29214..29477
                     /locus_tag="VYO12_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1587069.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29534..30259
                     /locus_tag="VYO12_000412"
     CDS             29534..30259
                     /locus_tag="VYO12_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1587070.1"
                     /translation="MEWIKKVEDSYAPFLTPFINPHQEKLLKILAKTYGLACSSSGEF
                     VSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHLTHAKILGTVINQLGIERKLF
                     GDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEERPFTEKIDKLEQYRELDLSV
                     SSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDKSDYTVQVGDLISVRKFGRLR
                     LLQDKGQTKKEKKKITVQLLLSK"
     gene            30268..31056
                     /gene="divIVA"
                     /locus_tag="VYO12_000413"
     CDS             30268..31056
                     /gene="divIVA"
                     /locus_tag="VYO12_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1587071.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPVPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31404..34196
                     /locus_tag="VYO12_000414"
     CDS             31404..34196
                     /locus_tag="VYO12_000414"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1587072.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKIIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
BASE COUNT        10674 a         6105 c         7524 g        10313 t
ORIGIN      
        1 aattgaaaac ggtaacaaaa ctaatttaga gaatgaaata tagagtattt ctctcttaaa
       61 agtttttggt gaaacgagat gtagaaagga gatttagcca aagagtctat tagtgctaga
      121 ataatagatt agaattattt tagaaaaacg aagtgagcag cttataaatt caagtcccca
      181 aatagattca tactagtatc ttttgcaaaa aataaagggc gacttccttc atgaatatca
      241 atttcatcta taaggaaggt agctaattga actaacttat ttattctgtt tgtcgctaga
      301 aaaatcagac ctccttgtga agattgagga gatacttaat gaaaatcaaa gaagaaacta
      361 gcaagctagt agcagattgc ccaaaacacc gctttgaggt tgtagataag actgacgtat
      421 ataatccaag gtgaagcgac tgtggtttga agagattttc aaagagtata ggctagagag
      481 tagtgttttt atgtccttct agtagaaaat gctagacaga agaatgggga acttggatag
      541 gaaaaataga ttgagaaagg aggttagaag agatgattat tacaaaaatt agccgtttag
      601 gaacttatgt gggagtaaat ccacattttg caacattaat agattttcta gaaaaaacag
      661 gactagaaaa tttaacagaa ggttcgattg ttatcgatgg taatcgattg tttgggaatt
      721 gatttactta tctagcagat ggtcaagcag gggctttctt tgaaacacac caaaaatatt
      781 tggatattca tttagttttg gaaaacgaag aagccatggc tgttacatcg ccggaaaatg
      841 taagcgttac ccaagaatat gatgaagaga aagatattga attatacaca gggaaagtgg
      901 aacagttggt tcatttgaga gctggcgaat gcctcatcac ttttccagaa gatttacatc
      961 aacccaaggt tcgtataaat gatgaacctg tgaaaaaagt tgtctttaaa gttgcgattt
     1021 cttaatgtag aaagagaaga acgatgaaaa aaatgagaaa gtttttatgt ctagctggaa
     1081 ttgcgctagc ggctgttgcc ttggtagctt gttcaggaaa aaaagaagct acaactagta
     1141 ctgaaccacc aacagaatta tctggtgaga ttacaatgtg gcactccttt actcaaggac
     1201 cccgtttaga aagtattcaa aaatcagcag atgctttcat gcaaaagcat ccaaaaacga
     1261 aaatcaagat tgaaacattt tcttggaatg acttctatac taaatggact acaggtttag
     1321 caaatggaaa tgtgccagat atcagtacag ctcttcctaa ccaagtaatg gaaatggtca
     1381 actcagatgc tttggttccg ctaaatgatt ctatcaagcg tattggacaa gataaattta
     1441 acgaaactgc cttaaatgaa gcaaaaatcg gagatgatta ctactctgtt cctctttatt
     1501 cacatgcaca agtcatgtgg gttagaacag atttgttaaa agaacataat attgaggttc
     1561 ctaaaacttg ggatcaactc tatgaagctt ctaaaaaatt gaaagaagct ggagtttatg
     1621 gcttgtctgt tccgtttgga acaaatgact taatggcaac acgtttcttg aacttctacg
     1681 tacgtagtgg tggaggaagc ctcttaacaa aagatcttaa agcagacttg acaagccaac
     1741 ttgctcaaga tggtattaaa tactgggtta aattgtataa agaaatctca cctcaagatt
     1801 ctttgaactt taatgtcctt caacaagcta ccttgttcta tcaaggaaaa acagcatttg
     1861 actttaactc tggcttccat atcggaggaa ttaatgccaa cagtcctcaa ttgattgatt
     1921 cgattgatgc ttatcctatt ccaaaaatca aagagtctga taaagaccaa ggaattgaaa
     1981 cctcaaacat tccaatggtt gtttggaaaa attcaaaaca tccagaagtt gctaaagcat
     2041 tcttagaagc actttataat gaagaagact acgttaaatt ccttgattca actccagtag
     2101 gtatgttgcc aactattaag gggattagcg attctgcagc ctataaagaa aatgaaactc
     2161 gtaagaagtt taaacatgct gaagaagtaa ttactgaagc tgttaaaaaa ggtactgcta
     2221 ttggttatga aaatggacca agtgtacaag ctggtatgtt gactaaccaa cacattattg
     2281 aacaaatgtt ccaagatatc attacaaatg gaacagatcc tatgaaagca gcaaaagaag
     2341 cagaaaaaca attaaacgat ttatttgagg ctgttcagta gatgtaaaag actagaaaat
     2401 aggtgggcta gtgagctgaa aagctctagc ccaatcttgt aaaagaaggg agaaggagaa
     2461 tggttaaaga acgtaattta actcgctgga tatttgtttt gccagctatg attatcgtag
     2521 gattactctt tgtttatccg tttttctcga gtatttttta tagctttacc aataagcatt
     2581 tgattatgcc taattataaa tttgttggtt tggctaacta taaagctgtg ctatcagatc
     2641 ccaacttctt taatgcgttc tttaattcaa ttaagtggac cgttttctca ttagttggtc
     2701 aagttttagt agggtttgta ttggctttag ctcttcacag agtacgccac ttcaagaaat
     2761 tatataggac cttattgatt gttccttggg catttcctac catcgttatt gccttttctt
     2821 ggcagtggat tctaaacggg gtttatggct acttacctaa tctaatcgta aaattaggtt
     2881 taatggaaca tacacctgca tttttgacag atagtacatg ggcattccta tgtttggtgt
     2941 ttatcaacat ttggtttgga gcaccaatga ttatggttaa tgtgctttca gctttgcaaa
     3001 cagtaccaga agaacaattt gaggctgcta agatagatgg tgcttcaagt tggcaggtgt
     3061 tcaagtttat cgtctttcca catattaaag tggttgtagg acttctagtt gttttgagaa
     3121 ctgtatggat ctttaataac tttgacatta tctacctcat tactggtggt ggaccagcca
     3181 atgctacaac gacgcttcca atttttgctt acaacctggg ttggggaact aaattgttgg
     3241 gtcgtgcttc agcagttaca gtattgctct ttatcttctt ggtggcgatt tgctttatct
     3301 actttgctat catcagtaag tgggaaaagg agggtagaaa ataatgaaga agaaatccag
     3361 tatttattta gatattctct cacatgtact tttagttggt gcgaccatcg ttgcaatttt
     3421 cccattggta tggattatca tatcttctgt caaagggaaa ggggaattaa ctcagtatcc
     3481 aacacgattt tggcctgaac agtttacatt agattatttc actcatgtta tcaacgattt
     3541 gcacttcatt gataacattc gaaacagttt aatcattgcc ttggctacaa ccctcattac
     3601 gattattatt tctgctatgg cagcctatgg tattgttcga ttctttccta aattgggagc
     3661 aatcatgtcg agactactcg tcattaccta tattttccca ccaattttgt tagcaattcc
     3721 ctattcaatt gccattgcta aagttgggtt aacaaatagt ttatttggct tgatgatggt
     3781 ttatctatct tttagtgttc catatgcagt ttggctctta gttggatttt tccaaacagt
     3841 tccaattgga attgaagaag cggctagaat tgatggtgca aataaatttg ttacgtttta
     3901 taaagttgtg ctaccgattg tagcaccagg tattgtagca acagctattt atacatttat
     3961 caatgcttgg aatgaattcc tgtatgcctt gattttgatt aacaatacag gaaagatgac
     4021 agtagcagta gcccttcgtt cacttaatgg ttcagaaata ctagactggg gagatatgat
     4081 ggcagcgtct gttattgtag ttcttccatc aattattttc ttctctatca tccaaaataa
     4141 gattgcaagt ggattatcag aaggatctgt gaagtagacg aaagaaggaa aaaaaatgaa
     4201 taaaagaggt ctttattcaa aactaggaat ttctgttgta ggcattagtc ttttaatggg
     4261 agttcctact ttgattcatg cgaatgaatt aaactatggt caactgtcca tatctcctat
     4321 ttttcaagga ggttcatatc aactgaacaa taagagtata gatatcagct ctttgttatt
     4381 agataaattg tctggagaga gtcagacagt agtaatgaaa tttaaagcag atgaaccaaa
     4441 ctctcttcaa gctttgtttg gcctatctaa tagtaaagca ggctttaaaa ataattactt
     4501 ttcaattttc atgagagatt ctggtgagat aggtgtagaa ataagagacg cccaaaaggg
     4561 aataaattat ttattttcta gaccagcttc attatgggga aagcataaag gacaggcagt
     4621 tgaaaataca ctagtatttg tatctgattc taaagataaa acatacacaa tgtatgttaa
     4681 tggaatagaa gtgttctctg aaacagttga tacatttttg ccaatttcaa atataaatgg
     4741 tatagataag gcaacactag gagctgttaa tcgtgaaggt aaggaacatt acctcgcaaa
     4801 aggaagtatt gatgaaatca gtctatttaa caaagcaatt agtgatcagg aagtttcaaa
     4861 tattcccttg tcaaatccat ttcagttaat tttccaatca ggagattcta ctcaagctaa
     4921 ctattttaga ataccgacac tatatacatt aagtagtgga agagttctat caagtattga
     4981 tgcacgttat ggtgggactc atgattctaa aagtaagatt aatattgcca cttcttatag
     5041 tgatgataat gggaaaacgt ggagtgagcc aatttttgct atgaagttta atgactatga
     5101 ggagcagtta gtttactggc cacgagataa taaattaaag aatagtcaaa ttagtggaag
     5161 tgcttcattc atagattcat ccattgttga agataaaaaa tctgggaaaa cgatattact
     5221 agctgatgtt atgcctgcgg gtattggaaa taataatgca aataaagccg actcaggttt
     5281 taaagaaata aatggtcatt attatttaaa actaaagaag aatggagata acgatttccg
     5341 ttatacagtt agagaaaatg gtgtcgttta tgatgaaaca actaataaac ctacaaatta
     5401 tactataaat gataagtatg aagttttgga gggaggaaag tctttaacag tcgaacaata
     5461 ttcggttgat tttgatagtg gctctttaag agaaaaacat aatggaaaac aggtccctat
     5521 gaatgttttc tacaaagatt cgttatttaa agtgactcct actaattata tagcaatgac
     5581 aactagtcag aatagaggag agagttggga acaatttaag ttgttgcctc cgttcttagg
     5641 agaaaaacat aatggaactt acttatgtcc cggacaaggt ttagcattaa aatcaagtaa
     5701 cagattgatt tttgcaacat atactagtgg agaactaacc tatctcattt ctgatgatag
     5761 tggtcaaaca tggaagaaat cctcagcttc aattccgttt aaaaatgcaa cagcagaagc
     5821 acaaatggtt gaactgagag atggtgtgat tagaacattc tttagaacca ctacaggtaa
     5881 gatagcttat atgactagta gagattctgg agaaacatgg tcgaaagttt cgtatattga
     5941 tggaatccaa caaacttcat atggcacaca agtatctgca attaaatact ctcaattaat
     6001 tgatggaaaa gaagcagtca ttttgagtac accaaattct agaagtggcc gtaagggagg
     6061 tcaattagtt gttggtttag tcaataaaga agatgatagt attgattgga aataccacta
     6121 tgatattgat ttgccttcgt atggttatgc ctattctgcg attacagaat tgccaaatca
     6181 tcacataggt gtgctgtttg aaaaatatga ttcgtggtcg agaaatgaat tgcatttaag
     6241 caatgtagtt cagtatatag atttggaaat taatgattta acaaaataaa ggagaaaaaa
     6301 catggttaaa tacggtgttg ttggagcagg gtattttgga gctgaattgg ctcgctacat
     6361 gcaaaagaat gatggagcag agattactct tctctatgat ccagataatg cagaggcgat
     6421 tgcagaagaa ttgggagcaa aagtagcaag ttccttagat gagttggttt ctagcgatga
     6481 agtagattgt gttatcgtcg caactccaaa taatcttcat aaggaaccgg ttattaaggc
     6541 tgcacagcat ggtaaaaatg ttttctgtga aaaaccaatt gcgctttctt atcaagattg
     6601 tcgcgagatg gtagatgcgt gtaaagaaaa caatgtaacc tttatggcag gacatattat
     6661 gaatttcttt aatggtgttc atcatgcaaa agaactcatt aatcaaggag ttatcggaga
     6721 cgttctatat tgtcatacag ctcgtaatgg ttgggaagaa caacaaccgt cagtatcatg
     6781 gaaaaaaatt cgtgaaaaat caggtggtca cttgtatcac cacatccatg aattggattg
     6841 cgttcaattc cttatggggg gcatgcctga aactgtaacc atgacaggtg gaaatgtggc
     6901 ccatgaaggt gaacatttcg gtgatgaaga tgatatgatt tttgtcaata tggaattttc
     6961 taataagcgt tttgccttgt tagaatgggg ttcagcttat cgttggggtg aacattatgt
     7021 cttaatccaa ggaagcaaag gtgccatccg cttagactta ttcaactgta aaggaactct
     7081 taagctagat gggcaagaaa gctatttctt gattcacgaa tcgcaagaag aagatgatga
     7141 tcggactcgt atctatcata gtacagagat ggatggagca attgcttatg gtaaaccagg
     7201 taaacgtact ccattatggc tatcatctgt cattgataaa gaaatgcgct atctgcatga
     7261 gattatggaa ggagccccag tatcagaaga atttgcaaaa cttttgacag gtgaagctgc
     7321 cctagaagca attgctactg cagatgcttg tacccagtct atgtttgaag atcgcaaagt
     7381 aaaattgtca gaaattgtaa aataaatttt ggtattctcc tatttatagg tcgacttgct
     7441 cctctgaaag tacttttaga ggagctgttt gactttgcta gtttttgaaa ctgaaatcta
     7501 ttatactaca aactattgaa agcgttttaa ttttaaggta taataatctc atagaaataa
     7561 agaaaaggag gaaagaggat gccacagatt agcaaagaag ccttgattga gcaaatcaaa
     7621 gatggaatca tcgtttcttg tcaggctctt cctcatgaac cgctttatac agaagcggga
     7681 ggggtgattc ccttgctggt caaagcggct gagcaaggtg gagcagtcgg tatccgagca
     7741 aacagtgttc gcgatatcaa ggaaattaag gaagtcacta aacttccaat cattgggatt
     7801 atcaaacgtg attatccacc tcaggaaccc ttcatcacgg ctactatgaa agaagttgat
     7861 gaattggcag aactggacat cgaggtgatt gctctggatt gtaccaagcg tgaacgctac
     7921 gatggtttgg aaattcaaga gttcattcgt caggttaagg agaaatatcc taatcagctt
     7981 ttgatggctg atactagtat cttcgaagaa gggctagcag ctgtagaagc aggaattgac
     8041 tttgtcggaa caaccttatc aggctacaca tcctacagtc caaaagtaga cggtccagat
     8101 tttgaattga ttaagaaact ctgtgatgcc ggtgtagatg tcattgcaga aggaaaaatt
     8161 catacaccag aacaagccaa acaaatcctt gaatatggag tgcgaggcat cgttgttggt
     8221 ggcgccatta ctagaccaaa agagattaca gaacgcttcg ttgctagtct taaataagat
     8281 gtgagaggga gttttatgtt taaagtttta caaaaagttg gaaaagcttt tatgttacct
     8341 atagctatac ttcctgcagc aggtctactt ttggggattg gtggtgcact ttcaaaccca
     8401 accacgatag caacttatcc aatactagac aatagtattt ttcaatcaat attccaagta
     8461 atgagctctg caggagaggt tgtattcagt aatttgtcac tacttctctg tgtgggatta
     8521 tgtattggct tagcgaaacg agataaagga accgctgcgt tagcaggagt aactggttac
     8581 ttagttatga ctgcaacgat caaagctttg gtaaaacttt ttatggcaga aggatctgca
     8641 attgatactg gagttattgg agcattagtt gtcggaatag ttgccgtata tttgcacaac
     8701 cgatataaca atattcaatt accttccgct ttaggattct ttggaggttc acgcttcgtt
     8761 cctattgtta catcgttctc ttctatcttg attggctttg tcttctttgt tatttggcca
     8821 cctttccaac aacttcttgt ttctacaggt ggatatattt ctcaggcggg tccaattgga
     8881 acttttctat atggattttt aatgagactt tctggagcag taggcttaca tcatataatt
     8941 taccctatgt tttggtatac tgaacttggt ggtgttgaaa ctgttgcagg acaaacagtg
     9001 gttggagctc aaaaaatatt ttttgctcaa ttagccgatc cggcccattc tggattattt
     9061 acagaaggaa caaggttttt tgcaggtcgt ttctcaacaa tgatgttcgg tttaccggct
     9121 gcctgtttag cgatgtacca tagtgttcct aaaaatcgtc gtaaaaaata cgcgggtttg
     9181 ttttttggag ttgctttaac atcttttatt accggtatta cagaaccaat tgaatttatg
     9241 tttctattcg tcagtccggt tctatatgtt gttcacgcat tccttgatgg tgttagcttc
     9301 tttattgcag acgtcttaaa tatttcaata ggaaacacat tttcaggagg tgtaatcgat
     9361 ttcactttat ttggaatttt gcaggggaac gctaagacga attgggttct tcagattcca
     9421 attggactta tttggagtgt tttgtattat attattttta gatggttcat tactcaattc
     9481 aacgttctaa cgccaggacg aggagaagaa gtagattcta aagaaatttc tgaatccgca
     9541 gattcaactt caaatactgc agattattta aaacaggata gcctacaaat tatcagagcc
     9601 ttgggtggat caaataatat agaagatgta gatgcttgtg tgacacgttt acgtgtagct
     9661 gtaaaagaag ttaatcaagt tgataaagca cttttaaaac aaattggtgc agttgatgtc
     9721 ttagaagtga agggtggcat tcaagcaatc tatggagcaa aagcaatctt atataaaaat
     9781 agtattaatg aaattttagg tgtagatgat taagtactta ctgacttaat aaaaaacaga
     9841 ggagagtgat ggatgagtag gatgaaatga aatcgtatac aagaaataaa gaactcatta
     9901 tccaagttgg atacgcttat tacataggag aatacaaatg aaatttagaa aattagcttg
     9961 tacagtactt gcgggtgctg tggttcttgg tcttgctgct tgtggcaatt ctggcggaag
    10021 taaagatgct gccaaatcag gtggtgacgg tgccaaaaca gaaatcactt ggtgggcatt
    10081 cccagtattt acccaagaaa aaactggtga cggtgttgga acttatgaaa aatcaatcat
    10141 cgaagcgttt gaaaaagcaa acccagatat aaaagtgaaa ttggaaacca tcgacttcaa
    10201 gtcaggtcct gaaaaaatca caacagccat cgaagcagga acagctccag acgtactctt
    10261 tgatgcacca ggacgtatca tccaatacgg taaaaacggt aaattggctg agttgaacga
    10321 cctcttcaca gatgaatttg ttaaagatgt caacaatgaa aacatcgtac aagcaagtaa
    10381 agctggagac aaggcttata tgtatccgat tagttctgcc ccattctaca tggcaatgaa
    10441 caagaaaatg ttagaagatg ctggagtagc aaaccttgta aaagaaggtt ggacaactga
    10501 tgattttgaa aaagtattga aagcacttaa agacaagggt tacacaccag gttcattgtt
    10561 cagttctggt caagggggag accaaggaac acgtgccttt atctctaacc tttatagcgg
    10621 ttctgtaaca gatgaaaaag ttagcaaata tacaactgat gatcctaaat tcgtcaaagg
    10681 tcttgaaaaa gcaactagct ggattaaaga caatttgatc aataatggtt cacaatttga
    10741 cggtggggca gatatccaaa actttgccaa cggtcaaaca tcttacacaa tcctttgggc
    10801 accagctcaa aatggtatcc aagctaaact tttagaagca agtaaggtag aagtggtaga
    10861 agtaccattc ccatcagacg aaggtaagcc agctcttgaa taccttgtaa acgggtttgc
    10921 agtattcaac aataaagacg acaagaaagt cgctgcatct aagaaattca tccagtttat
    10981 cgcagatgac aaggagtggg gacctaaaga cgtagttcgt acaggtgctt tcccagtccg
    11041 tacttcattt ggaaaacttt atgaagacaa acgcatggaa acaatcagcg gctggactca
    11101 atactactca ccatactaca acactattga tggatttgct gaaatgagaa cactttggtt
    11161 cccaatgttg caatctgtat caaatggtga cgaaaaacca gcagatgctt tgaaagcctt
    11221 cactgaaaaa gcgaacgaaa caatcaaaaa agctatgaaa caatagtcct tagttattct
    11281 ataaaaagta gttttttaaa gaacctaaga gtgtataccc ccttttccct ctacacagat
    11341 agtgtaagaa aagggggctt ttgtttaaaa tgtaagaaac tgtcacgaaa ttaaaatgaa
    11401 gttcttacat aagcgaatca taaaaaattt cattttgatt ttaaaacagt tcaagaaagt
    11461 caaaaaatta ttctatttga aagagaggtg ccgactgtga aagtcaataa aatccgtatg
    11521 cgggaaacag tgatttccta cgctttccta gcaccagtat tattcttctt tgtcatcttt
    11581 gtgttggctc cgatggtgat gggcttcatt acaagtttct ttaactactc aatgactaaa
    11641 tttgagtttg taggcttgga taactatatc cgtatgttta aagatcctgt ctttacaaaa
    11701 tctctgatta acacagttat tttggttatt ggatctgtac cagttgttgt tctattctca
    11761 ctctttgtag catctcagac ctatcatcaa aatgtcattg ccagatcctt ctaccgtttc
    11821 gtcttcttcc ttcctgttgt aacgggtagt gttgccgtga cagttgtttg gaaatggatt
    11881 tatgacccac tatcagggat tctaaacttt gtccttaagt caagccacat catcagccaa
    11941 aacatttctt ggttgggaga taaaaactgg gcattgatgg cgattatgat tattctcttg
    12001 accacttcag ttggtcagcc catcatcctt tatatcgctg ccatggggaa tattgacaat
    12061 tcactggttg aagcggcgcg tgttgatggt gcaactgagt ttcaagtttt ttggaagatt
    12121 aaatggccaa gccttcttcc aacaactctt tatattgcaa tcatcacaac aattaactca
    12181 ttccagtgtt tcgccttgat tcagcttttg acatctggtg gtccaaacta ctcaacaagt
    12241 accttgatgt actaccttta cgaaaaagcc ttccaattga cagaatacgg ctatgccaac
    12301 acaattggtg tcttcttggc agtcatgatt gctatcgtaa gctttgttca atttaaagta
    12361 cttggaaacg acgtagaata ctaaagaaag gagacagcta tgcaatctac agaaaaaaaa
    12421 ccattaacag cctttactgt tatttcaaca atcattttgc tcttgttgac tgtgctgttc
    12481 atctttccat tctactggat tttgacaggg gcattcaaat cacaacctga tacaattgtt
    12541 attcctcctc agtggttccc taaaatgcca accatggaaa acttccaaca actcatggtg
    12601 cagaaccctg ccttgcaatg gatgtggaac tcagtattta tctcattggt aaccatgttc
    12661 ttagtctgtg caacctcatc tctagcaggt tatgtattgg ctaaaaaacg tttctatggt
    12721 caacgcattc tatttgctat ctttatcgct gctatggcac ttccaaaaca agttgtcctt
    12781 gtaccattgg tacgtatcgt caacttcatg ggaatccacg atactctctg ggcagttatc
    12841 ttgcctttga ttggatggcc attcggtgtc ttcctcatga aacagttcag tgaaaatatc
    12901 cctacagagt tgcttgaatc agctaaaatc gacggttgtg gtgagattcg taccttctgg
    12961 agtgtagcct tcccgattgt gaaaccaggg tttgcagccc ttgcaatctt taccttcatc
    13021 aatacttgga atgactactt catgcaattg gtaatgttga cttcacgtaa caatttgacc
    13081 atctcacttg gggttgcgac catgcaggct gaaatggcaa ccaactatgg tttgattatg
    13141 gcaggagctg cccttgctgc tgttccaatc gtcacagtct tcctagtctt ccaaaaatcc
    13201 ttcacacagg gtattactat gggagcggtc aaaggataat actctgcgaa aatcgaatgc
    13261 aaactacgtc agcttcacct tgccatactt aagtattgcc tgtggttagc ttcctagttt
    13321 gttcttcaat tttcattgag tataggaaaa tcaatctatc aagatacaga agtatatttt
    13381 atagatttag agaatataga agttataagt gtctacaaaa tggagggtat gcagttactt
    13441 tatgaagttt tgtcagacac ttatcaactt aagaatggtt ttagttaact atcagaaacg
    13501 aaggaaagag tatgattttt gacgatttga aaaacatcac cttttacaaa gggattcatc
    13561 ccaatttaga caaggctatc gactacctct accaacatcg taaagattca ttcgaattag
    13621 gaaagtatga gattgatgga gataaagtct ttctagttgt tcaggaaaat gtcctcaatc
    13681 aagttgagaa taatcaattt gaacaccata agaactatgc agatttgcat ttgctgatag
    13741 aagggcatga atattcgagc tacggttcac gtatcaaaga cgaggcagta gcattcgacg
    13801 aagcgagtga cattggcttt gttcattgtt atgaacacta cccactctta ttgggttatc
    13861 acaattttgc gattttcttc ccaggtgagc cacatcagcc aaatggttat gcaggcatgg
    13921 aagaaaaggt tcgaaaatat ctctttaaaa tcttgattga ttaaaaatag gatgaattgt
    13981 ttttttgtaa agctttgata atactctacc atgaaattga tctttgtgag gtagagaaat
    14041 gagaataaaa tatttaaaaa ttggtatctt ctaagtatgc tgcaagagct agtttcttgg
    14101 atggacaggg aattacagtt gatgagatgg cttggataat taggggcatt gtgaatgcat
    14161 tgattggtag atacataaaa ttaggtgctt atgcggctaa gtatggtatt agtatggcac
    14221 gctcgatctt aagtagggca gctgcaactg cagcagcaag agtaggatta tcgaccaaga
    14281 tttctggatg gattttacga gtagctgtga atgtagctga tgtatatggt aattttgcct
    14341 acaatattgc tgcagcttgg gatgcacatg ataaaatccc taacaatggt cgtataaact
    14401 tttaaaatgc gagaatgaaa gcactttgta tttttttatt gaatatgtta gcttggacag
    14461 tgcttgcaat gataattcgt ggagggctag atggatttaa tgagcatact tggagtacta
    14521 ttttaattgc ttcgctgttc ggggtatatg attataagcc catagataaa aatagaaaaa
    14581 agtccaaaag aaaaaagaaa aaatagattt gttcatggta gggacttatg aaagctttac
    14641 tgacaaaaaa gaaaacagtt tacaaagaaa aatgatggag gagcaaacat ggcacaaaaa
    14701 ggagtaagcc ttatcaaggc agcatttgat acagacaact ttctcatgcg ttttagtgag
    14761 aaagtcttgg acatcgtgac agccaatctt ctttttgtcg tctcttgttt gcccatcgtg
    14821 acgattggag tggctaaaat cagcctctac gagaccatgt tcgaagttaa gaagagcaga
    14881 cgggtgcctg tttttaaaat ctatctaaga tctttcaagc aaaatctgaa actaggtctt
    14941 cagctgggtt taatggagtt aggaattgtg tttcttaccc tttcagatct ctatcttttc
    15001 tggggtcaaa cagctctgcc cttccaattg ctgaaagcca tttgtttagg tattctgatt
    15061 tttcttacta tcgtgatgct ggctagttac cctatcgcgg cacgttatga cctatcttgg
    15121 aaagaaattc ttcaaaaagg attgatgttg gctagtttta actttccttg gttcttcctc
    15181 atgttagcca ttcttgtcct cattgtgatg gttctttatc tgtccgcctt cagtctactc
    15241 ttaggtggct cagtcttcct actttttggg tttggactat tggtctttat ccagactgga
    15301 ttgatggaga aaattttcgc aaaataccaa taggagcttt atttctgaaa ctactttcaa
    15361 aggctccaaa cgctattcta taagcgagaa actaaaatct aagtgtctat aagatattga
    15421 aagccttttc gggaaagtgt atactaaaga agtaaagaaa aatctgcttt agcagaaaat
    15481 aaaaataata ggagaaaatc tatgtcagat ttgaaaaaat acgaaggtgt cattccagcc
    15541 ttctacgcat gttatgatga tcaaggagaa gtaagcccag aacgtacgcg tgccttggtt
    15601 caatacttca ttgataaagg tgttcaaggt ctttatgtca atggttcttc tggtgaatgt
    15661 atctaccaaa gcgttgaaga tcgcaagttg attttggaag aagtcatggc ggtagccaaa
    15721 ggtaaattga ccatcattgc tcatgttgcc tgcaacaata ctaaagacag tatggaactt
    15781 gctcgccatg ctgaaagctt gggagtagat gctattgcaa cgattccacc aatttacttc
    15841 cgcttgccag aatactcagt tgccaaatac tggaacgata tcagttctgc agctccaaac
    15901 acagactacg tgatttacaa cattcctcaa ttggcagggg ttgctttgac tccaagcctt
    15961 tacacagaaa tgttgaaaaa tcctcgtgtt atcggtgtta agaactcttc tatgccagtt
    16021 caagatatcc aaacctttgt cagccttggt ggggaagacc atatcgtctt taacggtcca
    16081 gatgaacagt tcctaggtgg ccgcctcatg ggtgctaagg ctggtatcgg tggtacttat
    16141 ggtgctatgc cagaactctt cttgaaactc aatcagttga ttgcggataa ggacctagaa
    16201 acagcgcgtg aattgcagta tgctatcaac gcaatcattg gtaaactcac ttctgctcat
    16261 ggaaatatgt acggtgtcat taaagaagtt ttgaaaatca atgaaggctt gaatattgga
    16321 tctgttcgtt caccattgac accagtgact gaagaagatc gtccagttgt agaagcggct
    16381 gctgccttga ttcgtgaaac caaggagcgc ttcctctaat ctaaaaggag gtatttatga
    16441 catactacgt tgcaattgat atcggtggaa ccaacatcaa gtatggtttg gttgatcaag
    16501 aggggcaact tcttgaatcg catgaaatgc caactgaggc gcataagggt ggacctcata
    16561 tcttacaaaa gaccaaagat atcgtagcta gttatttaga aaaaggccta gtagcaggtg
    16621 ttgccatatc ttctgctggg atggtggatc cggataaggg tgagattttc tatgctgggc
    16681 cgcaaatccc taactacgca ggcacccagt tcaaaaagga aatcgaagaa agctttacta
    16741 ttccttgtga gattgaaaat gatgtcaact gtgcaggtct tgctgaggca gtatctggtt
    16801 caggcaaggg agcaagtatg acactttgct tgaccattgg aacaggtatc ggtggttgct
    16861 tgattatgga taggaaagtc ttccatggtt ttagcaattc agcctgtgaa gtcgggtata
    16921 tgcatatgca ggatggagct tttcaagact tggcttctac aacagcttta gtgaaatatg
    16981 tagctgaagc ccatggagaa gatgttgatc agtggaatgg ccgtagaatt ttcaaagaag
    17041 ccactgaagg aaacaaaatc tgcatggaag gtattgaccg tatggttgac tatctaggaa
    17101 aaggtctggc aaatatttgc tacgttgcca atccagaagt ggttattctt ggtggtggta
    17161 tcatggggca agaggctatc ctcaaaccta agattcgcac agccttgaaa gaggctttgg
    17221 taccaagttt agcagaaaaa acacgattag aatttgccca tcaccaaaat acagcaggga
    17281 tgttgggtgc atattatcat tttaagacaa aacaatccta gtttggctca gccaaactag
    17341 gattttctta cacgtttttg tctacgatag ccgttgagtt ttttattttc ccagtagcta
    17401 ttaaagattt tttccttgct ttcgcgattg atttccaaaa agtaggcata aatcaaatcg
    17461 ataaagaaga gcataggaag ttgagcggat attcgttgga tataggaggg ttggctgtgg
    17521 gtggctacaa gaacagtctc tgtataggtc tggctatttt tattgggaac acttgtaaag
    17581 agtacagtct ttgcccccat ctccttggca tctaatagac tatctaaaat agaaggagtt
    17641 gagcctgaaa gtgagaagcc cagtactaga caattttcat ccatgatgct ggttgtccag
    17701 gcaaagccgt cttggtctgt caaagcttcg cagaccacgc ctagtcgcat aaaacgtaat
    17761 ttcatttcac gggcgacgag accagaactc cctgttccaa agaagtagat acgctcagca
    17821 tcttcgatta gctgggcaat tcgttctagt tggatttcgt caatcaagtc ttgtgtttgt
    17881 tccctcatat tgctataact tctgaggact cgtttggtca gtggactgtg cttggagact
    17941 tggttggctt gattttctgc ctgatgttgg tattggaaaa taaattctcg gtagccagta
    18001 aagccacact ttttagcaaa gcgggtcaaa gcagcttgag aaatatgtaa tttttgggtg
    18061 acttgttgag aagataaatc atctgtaatc gtttcagctt gcaaaaaata gcgagcgatt
    18121 tcttgctcta ggtctgtcat ttcttcaaaa tgtgaatcaa tgatagttgc gatatctggt
    18181 ttgtccatag ggaaagctcc tttacatgag tcatactgga agactagatc agggaatagt
    18241 cacacttcat tataacacat aatataagga tagataaata aaaacgcatt tctgttttaa
    18301 aaacgaaaaa atcgaaaaag cttctctctt ttccataatt ttctactcaa attgtggtac
    18361 aattaagagt aagattttaa gttagaaatg agactgattt gtatgagaaa atttaacagc
    18421 cattcgattc cgattcggct taatttattg ttttcaatcg tcattttact ctttatgacc
    18481 attattggtc gtttgttgta tatgcaggtt ttgaacaagg atttttacga aaaaaagcta
    18541 gcctcagcta gtcagaccaa gattacaagc agttcagccc gtggggaaat ttatgatgct
    18601 agtggaaaac ctttggtaga aaatacgtta aagcaggttg tttcctttac gcgtagcaat
    18661 aaaatgacgg ctacagactt aaaagaaaca gctaaaaagt tactgactta tgtgagcatc
    18721 agttctccaa atttgacaga acgccagctg gcggattact atttggctga tcctgaaatc
    18781 tataaaaaaa cagtggaagc tctcccaagc gagaaacgct tggattcaga tggaaatcgt
    18841 ctatccgaat cagaactgta taacaatgcg gtcgatagtg tccaaacaag tcaactaaac
    18901 tatacagagg atgaaaagaa agaaatctat ctttttagtc agttaaatgc tgttggaaac
    18961 tttgcgacag gaaccattgc tacagatcct ctaaatgatt ctcaggtggc tgttattgcc
    19021 tctatttcaa aggagatgcc tggcattagt atttctactt cttgggatag aaaggttttg
    19081 gaaacttccc tttcttctat agttgggagt gtatccagtg aaaaagctgg tctcccagcg
    19141 gaagaagcag aagcctatct taaaaaaggc tattctctaa atgaccgtgt aggaacctcc
    19201 tatttggaaa agcaatatga agagacctta caaggaaaac gatcggtaaa agaaatccat
    19261 ctggataaat atggcaatat ggaaagcgtg gatacaattg aggaaggtag taagggaaac
    19321 aatatcaaac tgaccattga tttggccttc caagatagcg tggatgcttt gctgaaaagt
    19381 tatttcaatt ctgagctaga aaatggtgga gccaagtatt ctgaaggtgt ctatgcagtc
    19441 gcccttaacc caaaaacagg tgcggttttg tctatgtcag ggattaaaca tgacttgaaa
    19501 acaggagagt tgacgcctga ttccttggga acggtaacca atgtctttgt tccaggttcg
    19561 gttgtcaagg cggcgaccat cagctcaggt tgggaaaatg gagtcttgtc aggaaaccag
    19621 accttgacag accagtccat tgtcttccaa ggttcagctc ccatcaattc ttggtatact
    19681 caggcttacg gttcattccc tatcacagcg gtccaagctc tggagtattc atcaaatacc
    19741 tatatggtcc aaacagcctt aggtcttatg gggcaaacct atcaacccaa tatgtttgtc
    19801 ggcaccagca atctagagtc tgctatggag aaactgcgtt caacctttgg cgaatatggc
    19861 ttgggtactg cgacaggaat tgacctacca gatgaatcta ctggatttgt tcccaaagag
    19921 tatagctttg ctaattacat tactaatgcc tttgggcagt ttgataacta tacgccgatg
    19981 cagttggctc agtatgtagc aactattgca aatgatggtg ttcgtgtggc tcctcgtatt
    20041 gttgaaggca tttatggtaa taatgataag ggaggactgg gtgacttgat tcagcaactg
    20101 caaccgacag agatgaataa ggtcaatata tccgactccg atatgagcat cttgcaccaa
    20161 ggtttttatc aggttgccca tggtactagt gaattgacaa ctggacgtgc cttttcaaat
    20221 ggtgccttgg tatccattag cggaaaaaca ggtacagccg aaagctatgt ggcagatggt
    20281 cagcaagcaa ccaataccaa tgcggtggcc tatgccccat ctgataatcc ccaaatcgct
    20341 gtcgcagtgg tctttcctca taataccaat ctaacaaatg gtgtaggacc ttccattgca
    20401 cgtgacatta tcaatctgta tcaaaaatac catccaatga actagaaagg aaattatgct
    20461 ttatccaaca cctattgcca agttgattga cagttattct aagttaccag gtatcgggat
    20521 taagacggct acgcgtctgg ccttttatac gattgggatg tctgctgatg atgtcaatga
    20581 atttgcaaaa aatctccttt ctgctaagag agaattgaca tattgttcta tttgtggacg
    20641 tttgacagac gacgatcctt gttctatctg tactgatccg actcgtgacc agacaacaat
    20701 tttagttctt gaggatagta gagatgtggc agccatggaa aatatccaag aataccatgg
    20761 actctatcat gtccttcatg gcctcatttc tcctatgaat ggtatcagtc cggacgatat
    20821 caatctcaag agccttatga ctcgtcttat ggatagtgag gtttcagaag tgattgtggc
    20881 gactaatgct acagcggatg gtgaagcgac ttccatgtat ctttcacgtt tgctcaagcc
    20941 ggctggtatc aaggttacgc gtctagcacg aggtctcgct gtgggagcgg acattgagta
    21001 tgcggacgaa gtgacactct tacgagccat tgaaaatcgg acagagttgt aagtgtaggc
    21061 aaatttacga actccattca tttataaaaa atcaaagagg ctggaaatcg ttcctatcgg
    21121 cctctttttg tatagtgtga tgagtaggat caggttcaag ttttaaaaaa ccaagcaaat
    21181 atgatatact aaagagcgag tattctagta gaattaggac aaataatatg aaacaaacga
    21241 ttattctttt atatggtgga cggagtgcgg aacgcgaagt ctctgtcctt tcagctgaga
    21301 gtgtcatgcg tgcggtcgat tacgaccgct tcacagtcaa gactttcttt atcagtcagt
    21361 caggtgactt tatcaaaaca caggaattta gtcatgctcc ggggcaagaa gaccgtctca
    21421 tgaccaatga aaccattgat tgggataaga aagttgcacc aagtgctatc tacgaagaag
    21481 gtgcagtggt ctttccagtc cttcacgggc caatgggaga agatggctct gttcaaggat
    21541 tcttggaagt tttgaaaatg ccttacgttg gttgcaacat tttgtcatca agtcttgcca
    21601 tggataaaat cacgactaag cgtgttctgg aatctgctgg tattgcccaa gttccttatg
    21661 tggctatcgt tgaaggcgat gatgtgactg ctaaaatcgc tgaagtggaa gaaaaattgg
    21721 cttatccagt cttcactaag ccgtcaaaca tggggtctag tgtcggtatt tctaagtctg
    21781 aaaaccaaga agaactccgt caagccttag aacttgcctt ccgatatgat agccgtgtct
    21841 tggttgagca aggagtgaat gcccgtgaaa ttgaggttgg cctcttgggt aactacgatg
    21901 tcaagagcac gctaccagga gaagttgtca aggacgttgc cttttatgac tacgatgcca
    21961 agtatattga taacaagatt actatggata ttcctgccaa aatcagtgat gatgtggtgg
    22021 ctgtcatgcg tcaaaatgca gaaacagcct tccgtgccat tggtggcctt ggtctatctc
    22081 gttgcgattt cttctataca gataagggag agatttttct caacgagctc aataccatgc
    22141 caggtttcac ccagtggtct atgtacccac tactttggga caatatgggg atcagctacc
    22201 cagaactaat cgagcgtttg gttgaccttg ccaaggaaag ttttgacaag cgcgaagcgc
    22261 atttgatata aaatgaaaga gagggtagaa gccagaacca tcactgcaag gtgactagag
    22321 ttctcggact tcagcccttt ttaaaggagt agaaatgaaa ttaacaatcc atgaaattgc
    22381 ccaagttgtt ggagccaaaa atgatatcag tatctttgag gacacccagt tagaaaaagc
    22441 tgagtttgat agtcgtttga ttggaactgg agatttattt gtgccactta aaggtgcgcg
    22501 tgatggccat gactttattg aaacagcctt tgaaaatggt gcagcagtaa ccttgtctga
    22561 gaaagaggtc tcaaatcatc cttacattct agtagatgat gttttgacag cctttcaatc
    22621 cctagcatcc tactatcttg aaaaaacgac tgttgatgtc tttgctgtta caggttcaaa
    22681 tggcaagaca acgactaagg atatgttggc gcatttactg tcaacaagat acaagaccta
    22741 caaaacacaa ggcaattaca ataacgagat tggccttcct tacacagttc ttcatatgcc
    22801 tgaagggaca gaaaagttgg tcttggagat ggggcaggat cacttgggag atattcatct
    22861 cttgtctgaa ctggctcgtc caaaaacagc catcgtgacc ttggttggag aagctcattt
    22921 ggcctttttc aaagatcgtt ctgagattgc taaaggaaaa atgcaaattg cggacggaat
    22981 ggcttcaggt tccttgcttt tagcgccggc tgaccctatc gtagaggact atttgccaac
    23041 tgataaaaaa gtggttcgtt ttgggcaagg ggcagagctg gaaattactg acttggttga
    23101 gcgcaaagat agtctgacct tcaaggccaa tttcttagag caagcccttg atttgccagt
    23161 aactggtaag tacaatgcta ccaatgctat gattgcatcc tatgttgccc tacaagaagg
    23221 agtttcagag gagcaaatcc gtttggcctt ccaaggtctt gaattgacgc gtaatcgtac
    23281 tgagtggaag aaagcagcca atggagcaga tatcctgtca gacgtatata atgccaatcc
    23341 aactgctatg aagctgattt tagagacttt ctctgccatt ccagccaatg aaggtggcaa
    23401 gaaaattgca gtgttggcgg atatgaagga gcttggtgac cagtctattc aactccataa
    23461 ccagatgatt ttgagccttt cgccagatgt gcttgatacc gttattttct atggagaaga
    23521 cattgctgaa ttagcccaat tggccagtca aatgttccca attggccacg tttactactt
    23581 caagaaaaca gaagaccaag accaatttga agacctagtc aagcaggtca aggaaagcct
    23641 cagtgccaat gatcaaatct tgctcaaggg ctctaactct atgaacctag ccatgttggt
    23701 agaaagttta gaaaatgaaa ccaagtgatt ttgttaagta tctgcaaaga atgattgccc
    23761 ttacagatac tggcttaacc tttacaaaag atccttttga ccgtgagcgc tacgaggact
    23821 tgcgaagtct gttatctgaa atgttgaatc agggatcaga cctggatgca gaagaagtag
    23881 cagaagtctt gaaaccaact tcagcttatg cgactccctt aatggacgtc cgtgcttgga
    23941 ttgttgagga tgagaagatt tgtctggtta ggggacaagg agaggatagt tgggctttgc
    24001 caggtggctt tggtgaagtc ggctattctc caactgaaaa tattctcaag gaaattgaag
    24061 aagaaaccgg ttttaaagcc aaagttgaaa gactgctagc tgtttttgat accaatcgtt
    24121 tccaactaca gagcaaacaa tatgcaaagt ttgtttttga atgtaagctt ctcgatggac
    24181 aattccaaga aaatcaagaa atcgctgacc ttcaattttt tgcaattgac caactgccga
    24241 atttatctga aaaacgcatt accaaggagc aaatagagct tctttggcag gtttatcaag
    24301 gtcagaggga gcaatatctt gactaagaag atgattatcg tatttctaaa ttcatttttc
    24361 ttaagtagca tggtataata aaatgcagga aaattttgaa tcatgaggaa gactagatga
    24421 atttatggga tattttcttt acgactcagg caaccgagcc gcccaaattt gacctttttt
    24481 ggtatgttag cctatttacg ctcttagcct taacctttta tacagcccat cgctatcgtg
    24541 aaaagaaggt ttaccaacga tttttccaaa tcttgcagac tgttcagtta atccttcttt
    24601 atggttggta ctgggtcaat catatgccac tgtcagaaag cctacccttt taccattgcc
    24661 gtatggctat gtttgtggta ctcttgcttc ctggtcagtc caaatataaa caatactttg
    24721 cattattggg aacatttggg acattagcag cctttgttta tccagtgcca gatgcttacc
    24781 cttttccaca tatcaccatt ctatccttta tctttggtca tttagcactc ttggggaact
    24841 ctctagttta tctattgaga cagtataatg cgcgattgct ggatgtgaag ggaatttttc
    24901 tcatgacctt tgccctaaat gccttgattt ttgtggtcaa tttggtgaca ggtggcgatt
    24961 acggattttt gacaaaaccg ccattggttg gggatcacgg tctagtagct aattatttac
    25021 ttgtttcaat tgtgctggta gctactatca gtttgactaa gaaaatctta gaattctttt
    25081 tagctcaaga agcagaaaaa atgattgcaa aggaagctta acacagagct ttcttttttg
    25141 ctcttagaga gtttttacaa gcagcttata aaataagaat ttctgaatag acaaactcaa
    25201 aaaatggctg ggaaatttag gaaaaaagca agcacgatta aattttttgt gttataatat
    25261 tttgtgaata gctatgccta tgtttagcta tggaataata cgaagtgcga aacttggaag
    25321 atagagagga agcgatgtaa tggctagaga aggctttttt acaggtctag atattggaac
    25381 aagctctgtc aaggtgcttg tggccgagca gagaactggt gaattaaatg taattggcgt
    25441 gagtaatgcc aaaagtaaag gtgtaaagga tggaattatt gttgatattg atgcagcagc
    25501 aactgctatc aagtcagcca tttcccaagc ggaagaaaag gcaggcattt cgattaaatc
    25561 agtaaatgtc ggcttgcctg gtaatctttt gcaggtagaa ccaactcagg ggatgattcc
    25621 agtaacatct gatactaagg aaattacgga tcaagatgtt gaaaatgttg tcaaatcagc
    25681 tttgacaaag agtatgacac ctgaccgtga agtcattacc tttattcctg aagaatttat
    25741 tgtggatggt ttccaaggga ttcgtgaccc acgtggcatg atgggggttc gccttgaaat
    25801 gcgtggtttg ctttatacag gacctcgtac tatcttgcac aatttgcgta agacggttga
    25861 gcgtgcaggt gttcaggttg aaaatgttat catttcacca ctagcaatgg ttcagtctgt
    25921 tttgaacgaa ggggaacgtg aatttggtgc tacagtgatt gatatggggg caggtcaaac
    25981 gactgtcgct acaatccgta atcaagaact ccagttcaca catattctcc aagaaggtgg
    26041 agattatgta actaaagata tctccaaggt tttgaaaacc tctcgcaaat tagcggaagg
    26101 cttgaaactg aattacgggg aagcctatcc gcctcttgca agcaaagaaa ccttccaagt
    26161 agaggttatt ggagaagtag aagcagtcga agtgacggaa gcctacttgt cagaaattat
    26221 ttctgcacga atcaagcaca tccttgaaca aatcaagcaa gaattagata gaaggcgtct
    26281 attggacctc cctggtggta ttgtcttaat cggtgggaat gccattttac caggtatggt
    26341 tgagcttgct caggaagtct ttggcgtccg tgtcaagctt tatgttccaa atcaagttgg
    26401 tatccgtaat ccagcctttg cgcatgtgat tagtttatca gaatttgcgg gtcaattaac
    26461 agaagttaat cttttggctc agggagcgat aaaaggtgag aatgacttaa gtcatcagcc
    26521 aattagtttt ggtgggatgc tgcaaaaaac agctcagttt gtacaatcaa cgcctgttca
    26581 accagctcct gctccagaag tagagccggt ggcgcctaca gaaccaatgg cggatttcca
    26641 acaagcttca caaaataaac cgaaattagc agatcgtttc cgtggcttga tcggaagcat
    26701 gtttgacgaa taaagaggaa aaataaatta tgacattttc atttgataca gctgctgctc
    26761 aaggggcagt gattaaagta attggtgtcg gtggaggtgg tggcaatgcc atcaaccgta
    26821 tggtcgacga aggtgttaca ggcgtagaat ttatcgcagc aaacacagat gtacaagcat
    26881 tgagtagtac aaaagctgag actgttattc agttgggacc taaattgact cgtggtttgg
    26941 gtgcaggagg tcaacctgag gttggtcgta aagccgctga agaaagcgaa gaaacactga
    27001 cggaagctat tagtggtgcc gatatggtct tcatcactgc tggtatggga ggaggctctg
    27061 gaactggagc tgctcctgtt attgctcgta tcgccaaaga tttaggtgcg cttacagttg
    27121 gtgttgtaac acgtcccttt ggttttgaag gaagtaagcg tggacaattt gctgtagaag
    27181 gaatcaatca acttcgtgag catgtagaca ctctattgat tatctcaaac aacaatttgc
    27241 ttgaaattgt tgataagaaa acaccgcttt tggaggctct tagcgaagcg gataacgttc
    27301 ttcgtcaagg tgttcaaggg attaccgatt tgattaccaa tccaggattg attaaccttg
    27361 actttgccga tgtgaaaacg gtaatggcaa acaaagggaa tgctcttatg ggtattggta
    27421 tcggtagtgg agaagaacgt gtggtagaag cggcacgtaa ggcaatctat tcaccacttc
    27481 ttgaaacaac tattgacggt gctgaggatg ttatcgtcaa cgttactggt ggtcttgact
    27541 taaccttgat tgaggcagaa gaggcttcac aaattgtgaa ccaggcagca ggtcaaggag
    27601 tgaacatctg gctcggtact tcaattgatg aaagtatgcg tgatgaaatt cgtgtaacag
    27661 ttgtcgcaac gggtgttcgt caagaccgcg tagaaaaggt tgtggctcca caagctagat
    27721 ctgctactaa ctaccgtgag acagtgaaac cagctcattc acatggcttt gatcgtcatt
    27781 ttgatatggc agaaacagct gaattgccaa aacaaaatcc acgtcgtttg gaaccaactc
    27841 aggcatctgc ttttggtgat tgggatcttc gccgtgaatc gattgttcgt acaacagatt
    27901 cagtcgtttc tccagtcgag cgctttgaag ccccaatttc acaagatgaa gatgaattgg
    27961 atacacctcc atttttcaaa aatcgttaag taaatgaatg taaaagaaaa tacagaactt
    28021 gtttttcgag aagttgcaga ggctagtctg agtgctcatc gagagagtgg ttcggtctct
    28081 gtcattgcag ttaccaagta tgtagatgta ccgacagcgg aagccttgct tccgctaggt
    28141 gttcatcata tcggtgaaaa tcgtgtagat aagtttctgg aaaaatatga agctttaaaa
    28201 gatcgagatg tgacttggca tttgattggt accttgcaaa gacgtaaggt gaaagatgtc
    28261 attcaatacg ttgattattt ccatgcattg gactcagtaa agctagcagg ggaaattcaa
    28321 aaaagaagtg accgagtcat caagtgtttc cttcaagtaa atatttctaa agaagaaagc
    28381 aaacacggtt tttcgagaga ggaactgctg gaaatcttgc cagagttagc cagactagat
    28441 aagattgaat atgttggttt aatgacgatg gcaccttttg aggctagcag tgagcagttg
    28501 aaagagattt tcaaggcgac ccaagattta caaagagaaa ttcaagagaa acaaattcca
    28561 aatatgccta tgaccgagtt aagtatggga atgagtcgtg attataaaga agcgattcaa
    28621 ttcggttcca cttttgttcg tataggtaca tcatttttta agtaggagag aaccatgtct
    28681 ttaaaagata gattcgatag atttatagat tattttacgg aggatgagga ttcaagtctc
    28741 ccttatgaaa aaagagatga gcctgtgttt actccagtaa attcttcaca ggaaccggct
    28801 ctcccaatga atcaaccttc acagtcggct ggtacaaaag agaacaatat caccagactt
    28861 catgcaagac aacaggaatt ggcaaatcag agtcagcgtg caacggataa ggtcattata
    28921 gatgttcgtt atcctagaaa atatgaggat gcaacagaaa ttgttgattt attggcagga
    28981 aacgaaagta tcttgattga ttttcagtat atgacagagg tgcaggctcg tcgttgtttg
    29041 gactatttgg atggagcttg tcatgtttta gctggaaatt tgaaaaaggt agcttctacc
    29101 atgtatttgt tgacaccagt gaatgttatt gtaaatgttg aagatatccg tttaccagat
    29161 gaagatcaac agggtgagtt cggttttgat atgaagcgaa atagagtacg ataatgattt
    29221 ttttaattcg tatgatttat aatgcagtgg atatttactc cctgattttg gtagccttcg
    29281 ctgtcatgtc ttggtttcca ggtgcctacg aatccagttt aggtcgttgg attgtagcgt
    29341 tggtgaaacc agtgcttgct cccttgcaac gcctgccttt acagatagcg ggtcttgatt
    29401 tatctgtttg ggtcgcgatt gttttggttc gatttttagg agaaaaccta gtgcgttttc
    29461 tggcgatgat aggatgaata aagggattta ttagcatttc tccatagaag atcgtccatt
    29521 tcttgacaag ggaatggaat ggataaagaa ggtagaagat agctatgctc cttttttaac
    29581 tccttttatc aatcctcatc aggagaagct attaaagatt ttggccaaaa cctatggtct
    29641 tgcttgtagc agtagtgggg aattcgtctc gagtgagtat gttcgagttt tattataccc
    29701 agattatttc caaccagagt tttcagattt tgaaatatct ctccaggaaa ttgtgtattc
    29761 caataaattt gaacatttaa cgcatgctaa gattttaggg acagtcatca atcaattagg
    29821 gattgaacgg aaactttttg gagatatcct agtagatgaa gaacgggcgc agattatgat
    29881 taatcagcag tttcttcttc tctttcaaga tggactaaag aaaattggtc gtatacctgt
    29941 ttcgctggag gaacgtcctt tcaccgagaa aatagataag ctagaacagt atcgagaact
    30001 ggatttatct gtgtctagtt ttcgattaga tgttctttta tcaaatgttt tgaaactatc
    30061 taggaatcaa gcaaaccagt tgattgaaaa gaaacttgtc caagtaaatt atcatgtggt
    30121 agacaaatca gattacactg ttcaagttgg agacttgatt agtgtgagaa aatttggtcg
    30181 cttgagatta cttcaagata agggacaaac gaaaaaagag aagaaaaaaa taaccgtcca
    30241 gttattatta agtaagtgag gaatagaatg ccaattacat cattagaaat aaaggacaag
    30301 acttttggaa ctcgattcag aggttttgat ccagaagaag tcgatgaatt tttagatatt
    30361 gtggttcgtg attacgaaga tcttgtgcgt gcgaatcatg ataaaaattt gcgtattaag
    30421 agtttagaag agcgtttgtc ttactttgat gaaataaaag attcattgag ccagtctgta
    30481 ttgattgctc aggatacagc tgagagagtg aaacaggcgg cgcatgaacg ttcaaacaat
    30541 atcattcatc aagcagagca agatgcgcaa cgcttgttgg aagaagctaa atataaggca
    30601 aacgagattc ttcgtcaagc aactgataat gctaagaaag tcgctgttga aacagaagaa
    30661 ttgaagaaca agagccgtgt cttccaccaa cgtctcaaat ctacaattga gagtcagttg
    30721 gctattgttg aatcttcaga ttgggaagat attctccgtc caacagctac ttatcttcaa
    30781 accagtgatg aagcctttaa agaagtggtt agcgaagtac ttggagaacc gattccagtt
    30841 ccaattgaag aagaaccaat tgatatgaca cgtcagttct ctcaagcaga aatggaagaa
    30901 ttacaagctc gtattgaggt agccgataaa gaattgtctg aatttgaagc tcagattaaa
    30961 caggaagtgg aagctccaac tcctgtagtg agtcctcaag ttgaagaaga gcctctgctc
    31021 atccagttgg cccaatgtat gaagaaccag aagtagctcc agtgcatccg acaggtccaa
    31081 caccagctac agaaactgtt gattcagcac cgggatttga agcaccgcaa gaatctgtta
    31141 caattttata agaaatattc tgagaacaat atcttatcct tatattccca gcgagcagga
    31201 gatggtgtga gtcctgcatt ccctatcgat aagattatcc tctcaaaaac tcaagtctga
    31261 atctagtaag atttgacgtt ccccacgtta cgggataaga gggagaaaga ctaaaacttt
    31321 ttccgaataa aggtggtacc acgattttcg tcctttttgg aagttgtggt ttttaatttg
    31381 ttattattta taaaggagat accatgaaac tcaaagacac ccttaatctt gggaaaactg
    31441 aattcccaat gcgtgcaggc cttcctacca aagagccagt ttggcaaaag gaatgggaag
    31501 atgcaaaact ttatcaacgt cgtcaagaat tgaaccaagg aaaacctcat ttcaccttgc
    31561 atgatggccc tccatacgct aacggaaata tccacgttgg acatgctatg aacaagattt
    31621 caaaagatat tattgttcgt tctaagtcta tgtcaggatt ttacgcacca tttattcctg
    31681 gttgggatac tcatggtctg ccaatcgagc aagtattgtc aaaacaaggt gtcaaacgta
    31741 aagaaatgga cttggttgag tacttgaaac tttgccgtga gtacgctctt tctcaagtag
    31801 ataaacaacg tgaagatttt aaacgtttgg gtgtttctgg tgactgggaa aatccatatg
    31861 tgaccttgac tcctgactat gaagcagctc aaattcgtgt atttggtgag atggctaata
    31921 agggttatat ctaccgtggt gccaagccag tttactggtc atggtcatct gagtcagccc
    31981 ttgctgaagc agagattgaa taccatgact tggtttcaac ttccctttac tatgccaaca
    32041 aggtaaaaga tggcaaagga gttctagata cagatactta tatcgttgtc tggacaacga
    32101 ctccatttac catcacagct tctcgtggtt tgacggttgg tgcagatatt gattacgttt
    32161 tggttcaacc tgctggtgaa gctcgtaagt ttgtcgttgc tgctgaatta ttgactagct
    32221 tgtctgagaa atttggctgg gctgatgttc aagttttgga aacttaccgt ggccaagaac
    32281 tcaaccacat cgtaacagaa cacccatggg atacagctgt agaagagttg gtaattcttg
    32341 gtgaccacgt tacgactgac tctggtacag gtattgttca tacagcccct ggttttggtg
    32401 aggacgatta caatgttggt attgctaata atcttgaagt cgcagtgact gttgatgaac
    32461 gtggtatcat gatgaagaat gctggtcctg aatttgaagg tcaattctat gaaaaggtag
    32521 ttccaactgt gattgaaaaa cttggtaacc tccttcttgc ccaagaagaa atctctcact
    32581 catatccatt tgactggcgt actaagaaac caatcatctg gcgtgcagtt ccacaatggt
    32641 ttgcctcagt ttctaaattc cgtcaagaaa tcttggacga aattgaaaaa gtgaaattcc
    32701 actcagaatg gggtaaagtc cgtctttaca atatgatccg tgaccgtggt gactgggtta
    32761 tctctcgtca acgtgcttgg ggtgttccac ttcctatctt ctacgctgaa gatggtacag
    32821 ctatcatggt agctgaaact attgaacacg tagctcaact ttttgaagaa catggttcaa
    32881 gcatttggtg ggaacgtgat gccaaagacc tcttgccaga aggatttact catccaggtt
    32941 caccaaacgg cgagttcaaa aaagaaactg atatcatgga cgtttggttt gactcaggtt
    33001 catcatggaa tggagtggtg gtaaaccgtc ctgaattgac ttacccagcc gacctttacc
    33061 tagaaggttc tgaccaatac cgtggttggt ttaactcatc acttatcaca tctgttgcca
    33121 accatggcgt agcaccttac aaacaaatct tgtcacaagg ttttgccctt gatggtaaag
    33181 gtgagaagat gtctaaatct cttggaaata ccattgctcc aagcgatgtt gaaaaacaat
    33241 tcggtgctga aatcttgcgt ctctgggtaa caagtgttga ctcaagcaat gacgtgcgta
    33301 tctctatgga tattttgagc caagtttctg aaacttaccg taagattcgt aacactcttc
    33361 gtttcttgat tgccaataca tctgacttta acccagctca agatacagtc gcttacgatg
    33421 agcttcgttc agttgataag tacatgacga ttcgctttaa ccagcttgtc aagatcattc
    33481 gtgatgccta tgcagacttt gaattcttga cgatctacaa ggccttggtg aactttatca
    33541 acgttgactt gtcagccttc taccttgatt ttgccaaaga tgttgtttac attgaaggtg
    33601 ccaaatcact ggaacgccgt caaatgcaga ctgtcttcta tgacattctt gtcaaaatca
    33661 ccaaactctt gacaccaatc cttcctcaca ctgcggaaga aatttggtca tatcttgagt
    33721 ttgaaacaga agacttcgtc caattgtcag aattaccaga ggctcaaact tttgctaatc
    33781 aagaagaaat cttggataca tgggcagcct tcatggactt ccgtggacaa gctcaaaaag
    33841 ccttggaaga agctcgtaat gcaaaagtaa tcggtaaatc acttgaagca cacttgacag
    33901 tttatccaaa cgaagttgtg aaaactctac tcgaagcagt aaacagcaat gtggctcaac
    33961 ttttgatcgt gtcagacttg accatcgcag aaggaccagc tccagaagct gcccttagct
    34021 tcgaagatgt agccttcaca gttgaacgcg ctgcaggtga agtatgtgac cgttgccgtc
    34081 gtattgaccc aacaacagca gaacgtagct accaggcagt tatctgtgac cactgtgcaa
    34141 gtatcgtaga agaaaacttt gcagatgcag tcgcagaagg atttgaagag aaataagatt
    34201 gaaaagtcta ggcaaaattc aatttgagaa gaaaagacaa ctaattttat agtctattaa
    34261 acgcattgta tcacgttttt gaacacctga tatgatgcgt tttttatttt taaaatttgc
    34321 aaggtgtgac tttttatact catactcaat gaaaatcaaa aagcaaacta ggaagctagc
    34381 cgcaggctgc tcaaaacact gttttgaggt tgtagataag actgacgaag tcagtaacat
    34441 atatatggta aggcgacgct gacgtggttt gaagagattt tcgaagagta tcaacaagaa
    34501 tcaaagagaa acttagcaag ctaacagtag taagataaaa taggaatttg atattaggga
    34561 taagattggt aaatagtgta atatttttac aacaataaat ttatatagtt atttct
//
LOCUS       DAVLXF010000009        34322 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLXF010000009 DAVLXF010000000
VERSION     DAVLXF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.009"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(97..499)
                     /locus_tag="VYO12_000415"
                     /pseudo
     CDS             complement(97..499)
                     /locus_tag="VYO12_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(575..1015)
                     /locus_tag="VYO12_000416"
     CDS             complement(575..1015)
                     /locus_tag="VYO12_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1587073.1"
                     /translation="MKAPIPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLREKLLEQALLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQRRSGSVVQEFLGNYSGYMHCDMLRQ"
     gene            1006..1313
                     /locus_tag="VYO12_000417"
                     /pseudo
     CDS             1006..1313
                     /locus_tag="VYO12_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1336..1686)
                     /gene="tnpB"
                     /locus_tag="VYO12_000418"
     CDS             complement(1336..1686)
                     /gene="tnpB"
                     /locus_tag="VYO12_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1587074.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(1667..1852)
                     /locus_tag="VYO12_000419"
     CDS             complement(1667..1852)
                     /locus_tag="VYO12_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587075.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2156..3718)
                     /gene="guaA"
                     /locus_tag="VYO12_000420"
     CDS             complement(2156..3718)
                     /gene="guaA"
                     /locus_tag="VYO12_000420"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1587076.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3860..4558
                     /locus_tag="VYO12_000421"
     CDS             3860..4558
                     /locus_tag="VYO12_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1587077.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQISYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4601..5497)
                     /locus_tag="VYO12_000422"
     CDS             complement(4601..5497)
                     /locus_tag="VYO12_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1587078.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILHIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5506..6441)
                     /locus_tag="VYO12_000423"
     CDS             complement(5506..6441)
                     /locus_tag="VYO12_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1587079.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSAPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARFLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6438..7163)
                     /locus_tag="VYO12_000424"
     CDS             complement(6438..7163)
                     /locus_tag="VYO12_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV1587080.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7247..7882)
                     /locus_tag="VYO12_000425"
     CDS             complement(7247..7882)
                     /locus_tag="VYO12_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1587081.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEKYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(7884..8711)
                     /locus_tag="VYO12_000426"
     CDS             complement(7884..8711)
                     /locus_tag="VYO12_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1587082.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8733..9659)
                     /locus_tag="VYO12_000427"
                     /pseudo
     CDS             complement(8733..9659)
                     /locus_tag="VYO12_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            10310..10921
                     /locus_tag="VYO12_000428"
     CDS             10310..10921
                     /locus_tag="VYO12_000428"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1587083.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            10966..11694
                     /gene="rlmB"
                     /locus_tag="VYO12_000429"
     CDS             10966..11694
                     /gene="rlmB"
                     /locus_tag="VYO12_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1587084.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(11733..12644)
                     /gene="trxB"
                     /locus_tag="VYO12_000430"
     CDS             complement(11733..12644)
                     /gene="trxB"
                     /locus_tag="VYO12_000430"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1587085.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12710..12934)
                     /locus_tag="VYO12_000431"
     CDS             complement(12710..12934)
                     /locus_tag="VYO12_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1587086.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAGARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13012..13755)
                     /locus_tag="VYO12_000432"
     CDS             complement(13012..13755)
                     /locus_tag="VYO12_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1587087.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13755..14555)
                     /locus_tag="VYO12_000433"
     CDS             complement(13755..14555)
                     /locus_tag="VYO12_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1587088.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14713..15069)
                     /locus_tag="VYO12_000434"
     CDS             complement(14713..15069)
                     /locus_tag="VYO12_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1587089.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLSVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15082..15594)
                     /locus_tag="VYO12_000435"
     CDS             complement(15082..15594)
                     /locus_tag="VYO12_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1587090.1"
                     /translation="MQKKYVKILYSSPIGILLLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15594..16088)
                     /locus_tag="VYO12_000436"
     CDS             complement(15594..16088)
                     /locus_tag="VYO12_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1587091.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16223..16669
                     /locus_tag="VYO12_000437"
     CDS             16223..16669
                     /locus_tag="VYO12_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1587092.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMINNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16666..17313
                     /locus_tag="VYO12_000438"
     CDS             16666..17313
                     /locus_tag="VYO12_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1587093.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKNVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIVYYM"
     gene            complement(17334..17915)
                     /gene="pdxT"
                     /locus_tag="VYO12_000439"
     CDS             complement(17334..17915)
                     /gene="pdxT"
                     /locus_tag="VYO12_000439"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1587094.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLLIREAILSGLPVFGTCAGLILLAKEITSQKESYLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(17916..18791)
                     /gene="pdxS"
                     /locus_tag="VYO12_000440"
     CDS             complement(17916..18791)
                     /gene="pdxS"
                     /locus_tag="VYO12_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1587095.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(18943..20322)
                     /locus_tag="VYO12_000441"
     CDS             complement(18943..20322)
                     /locus_tag="VYO12_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1587096.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20616..21539
                     /locus_tag="VYO12_000442"
     CDS             20616..21539
                     /locus_tag="VYO12_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1587097.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     IFWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21599..22204
                     /locus_tag="VYO12_000443"
     CDS             21599..22204
                     /locus_tag="VYO12_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1587098.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22223..23467
                     /locus_tag="VYO12_000444"
     CDS             22223..23467
                     /locus_tag="VYO12_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1587099.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(23583..23840)
                     /locus_tag="VYO12_000445"
     CDS             complement(23583..23840)
                     /locus_tag="VYO12_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1587100.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(23882..25918)
                     /locus_tag="VYO12_000446"
     CDS             complement(23882..25918)
                     /locus_tag="VYO12_000446"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1587101.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKEIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKKIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26130..27047)
                     /gene="glyQ"
                     /locus_tag="VYO12_000447"
     CDS             complement(26130..27047)
                     /gene="glyQ"
                     /locus_tag="VYO12_000447"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1587102.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(27239..28000)
                     /locus_tag="VYO12_000448"
     CDS             complement(27239..28000)
                     /locus_tag="VYO12_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1587103.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFFTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKIFPLLDWFVQQKLIEKEI"
     gene            complement(28273..29115)
                     /locus_tag="VYO12_000449"
     CDS             complement(28273..29115)
                     /locus_tag="VYO12_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1587104.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(29229..30620)
                     /locus_tag="VYO12_000450"
     CDS             complement(29229..30620)
                     /locus_tag="VYO12_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1587105.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKSFSDQDLSAWNFDYKDS
                     QIILYSSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(30855..31232)
                     /locus_tag="VYO12_000451"
     CDS             complement(30855..31232)
                     /locus_tag="VYO12_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1587106.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMSYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            31465..32442
                     /locus_tag="VYO12_000452"
     CDS             31465..32442
                     /locus_tag="VYO12_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1587107.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYSKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGTLFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQDPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            32439..33521
                     /locus_tag="VYO12_000453"
     CDS             32439..33521
                     /locus_tag="VYO12_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1587108.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTLIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGSEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(33585..34322)
                     /locus_tag="VYO12_000454"
                     /pseudo
     CDS             complement(33585..34322)
                     /locus_tag="VYO12_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT        10177 a         7694 c         6182 g        10269 t
ORIGIN      
        1 agctcttgct ttagctcctt caaaactttg agaaaaaacc accctcgctt ttttaaactg
       61 agcttaggct aaaagagtcc agtggactct tttactccgt cccataacca atgatttaat
      121 ggcgcgttca gctagattat tggaaaggac cagatgtccg tcttttaaaa tggtcttaaa
      181 ggtttcttca tacttgaggc tgtattcaat tgcccttcct ggttttgaac ctgctaaaac
      241 tgactgacgg cggcaccaag caaagaagtc ttccattaag ggctggacgt ttctgtagtc
      301 gttcatcagc tggcaaagcc tcccagtctc tttccaaggc aaataactgg tcacgatagg
      361 ctaaaccttt agctcctaag gatgatttat ctgcttgctt gggggtcgct tcaaaaaatt
      421 tccttctcac atgcgcccaa catccaacaa gttcagcttc ttccagttga cgataagctt
      481 ctagcggttc gcagtttacc aagcttagcg tcgccttgct cctaaacctt agactgctat
      541 cgcataggca gaactagagg actaaagttc taagttactg ccgcaacata tcacaatgca
      601 tatagccaga ataatttcct aggaattctt gcactaccga accactccga cgctgatcat
      661 ggtggtacag cgtaatccct tgtttctcag ctttcccaga caaaaaagtc caatagtaag
      721 tcagctgact atcactttct aagacccgat aagaggtttc atctgcatga agaagagctt
      781 gttctaacaa cttttctcgt aaaaggttat aaaggggctc caaatagtat tgactcgtct
      841 tgatatgcca attagagatt tccttacgtg tgattggtaa acccatccta gcccaatctt
      901 cttcttggcg ataattgggt accttcagat taaacttctg atggatggtg tgagcgataa
      961 tagaagctga gccaaggcta tgcgccaaag gggctttagg aataggagct ttcacaagct
     1021 tatccagatg attatctttt actcgttatg gacaatgcta tatggcataa atcaagtacc
     1081 ttaaagattc cgactaatat tggctttgca tttattcctc catacacacc agagatgaac
     1141 ccattgaaca agtgtggaaa gagattcgta aacgtggatt taagaataaa gcctttcaaa
     1201 ctttggaaga tgtcatgaat caacttcaag atgttataca aggactggag aaggaggtga
     1261 taaagtccat cgttaatcgg agatggacta gaatgctttt tgaaagcaga tgagtataaa
     1321 aagaaagtcc tcatttcaat agaaatcacg actttctgat aaatttattt tgggagtgat
     1381 agaaaagccc ttcataagcc aatctacttg ttcaggtgtg agagctttga catccttttc
     1441 tgtactgggc caagtcagtc tgccgttctc aaagcgttta tatagtagcc aaaatccttg
     1501 accatcccag taaagggctt taaagcggtc tttacgtcca ccacaaaaga gaaaaacttg
     1561 accggagaaa ggatccaatt caaagtgggt tttaaccaga taagccagtg aatcgattcc
     1621 ttgtctcata tcagtcttcc cacacacgag atagacctgc cctaggctag atagatgaat
     1681 tgtcatagag caacacctta tccaaaagct gttctaccat ttcgagattg agagattgaa
     1741 aaaaacttac ttcaagcttg ccaatacgaa ttttaagcag aatatcattt ctctttttag
     1801 aatcaaaagg acgagattgt ggaatctcta caggaacaat aggttgtggc attaaaatat
     1861 cctccaatag ttttacttta ctaatagtat acaggaggaa ctccatgaat gatagatacc
     1921 ttgttatgac gcgcttacta tattagaaag ttggctctct aattaaatta attctcatta
     1981 tatatatcaa aagaccgcag tttgaaaact acggtctaat tttttgtcca aatgtaaatc
     2041 acttggaact attattcaat ttattctttt tagtaaatgt tcccgtttta atcccagtta
     2101 cagtttttaa tcctctgtaa ccgttgattt tacaaagctt ttaatttttt tgcgattact
     2161 cccactcaac tgttgcaggt ggtttacttg taatatcgta gacgatacgg ttaacatgat
     2221 ccacttcatt tacgatacgt actgagattt tttgaagtac ttcccatgga attttggcaa
     2281 aatcagcagt cataccatcg atagaagtga tagcacggat tgcaatcgtg tagtcatacg
     2341 tacgaccgtc acccataaca ccgactgaac gaacgcctgt gttaacagtg aagtattgcc
     2401 aaatatcgcg gtcaagtcca gctttagcga tttcttcacg aagaatagcg tctgattcac
     2461 gaacggtttc aagtttctct tcagtgattt cacccatgac acggatagca agtcctggtc
     2521 ctgggaatgg ttggcgccat acgatatggt ctggcatacc aagctctgta ccaagagcac
     2581 gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa ttcaaactgc atatcttctg
     2641 gaagaccacc cacgttgtgg tgtgacttga tagtttgagc tgtatccgta ccagactcga
     2701 taacatctgt atataaagta ccttgagcaa ggaatttcac atctttgagc ttgcttgctt
     2761 cgtcatcgaa tacatagaca aactcgttac caatgatttt acgtttttgt tcaggatcag
     2821 aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc tgctttgacg atattcaaac
     2881 caaacttacc accgagcatg tccataactt gatcagcttc gcctttacga agaagaccgt
     2941 ggtctacgaa gatacagatc aattgatcgc caatcgcttt ttggagaaga accccaacga
     3001 cagatgagtc aacaccacct gatagaccaa gaaggacacg tttatcaccg acggtttcac
     3061 gaattttttt gatctgcatg tcaatgaaat tatccattga ccagtcacct ttagccttac
     3121 aaatgttaag ggcaaagtta cgaaggatat catttccgta tacagaatga cgaacttctg
     3181 ggtggaattg gataccgtaa atgtgtttat ctgggttttc gatggctgcg tatgggcagt
     3241 cagctgatgt acctgtacga acaaagtcag caggaatctc agtaaccgca tcaccatggc
     3301 tcatcaaaac agtctgttca tcaggtgttg attcaaaaag cgctgatggt gtgtgagtta
     3361 gggttgattg accgtattca cgatttccag catcacctgc aggaacaact tttcctccaa
     3421 gtttatgggt caataactgc ataccataac agattcccaa aattggaatt ccgagttcga
     3481 agatttctgg gtcaatatca aatgaaccat cttcatatac agaatttgga ccacctgata
     3541 gaataattcc tacaggattg acttcacgaa cttcagcagc tgaaatttta tggcttttta
     3601 gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat cagctggttg tactggctac
     3661 catagtccaa tacgatgatt ttttctacat cttgcaaatc agttgaaatg ttgctcatct
     3721 ttttcctttc tcaaaagaaa tatcttttct caatattcta tcacaaataa gggcgaatta
     3781 ccaactaaac aaggcgaagt ttgacttttt ctagtttatt ggggtacaat agagaaaaga
     3841 tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa atccatgatc agataaaaaa
     3901 ggatattgac gagcaccgtt gggctattgg tgagaggctt cccagtgaaa gagatttagc
     3961 tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc gtatctctat tagtcgaaga
     4021 aggcgtctta gagcgccgtg taggaagcgg cacctttgtt tccagtactc gagtacaaga
     4081 aaagatgcga gggacaacca gttttactga aattgtcaaa tcccaaggta aagttccctc
     4141 tagccagctc atttcctaca aaaaaaccat tcccaatgag caagaagttg ccaagctagg
     4201 aatttttcca acggacaata ttatccgaat ggagcgggtc cgctatgccg accaagttcc
     4261 cctagtttat gaagttgctt ctattcctga aaaattcatt aaggacttta aaaaagaaga
     4321 aatcaccagt catttcttcc aaaccttgca aaaacatggc tatcgtatcg gtaaatccca
     4381 acagaccatc tatgctcgcc ttgctaaaga aaagattgcc cactatttgg aagttgaaaa
     4441 aggacatgct attcttggat tgacccagat ttcctaccta gaagatggta ctgcttttga
     4501 atacgtaaaa agtcaatatg taggcgaacg ctttgaattt tatcttgaaa ataattagat
     4561 taccacgcaa aaactctctg tcacggacaa agagtctttt ttatttttct aagaataaat
     4621 ctttcaaaga aatcaaagtt acagcaaaca atatcattgc cataccaaga aaatcaatgg
     4681 gataaaattg ttcattcatt attaagaagg caaagaaaat cgccgatatt ggctcaattg
     4741 aagccaacaa gcttgacttg accggtccta tcagactggc tcctttaagg aaagctgtat
     4801 aggcaaagac agtcccgata aggataatgc ctgcaaacgc aaggagaaaa tcaagactag
     4861 tcgggatatc ggcctgtaga acccctgtaa aaggaagggc gaccaaacct gctatgacca
     4921 ttcccacacc aatgaccaag ctgctccccc actttttaat caaggctatg tgtaaaatga
     4981 tatacaaagc ataagtcaag gcagaaaaga gaccccagaa cagaccagca ggtgtcatgg
     5041 ataactggtc caactgccca tgtgttgcga tcaggaaggt tcctccgatg gcgaatatga
     5101 tggaaactat ctctcccagt gtcggtgcca ccctatcctt gatacagcta taaattaaaa
     5161 ttccgacagg acaaacatac tgaagcaccg tcgctgttcc cgcattggtc tcctgaatag
     5221 cagacagata ggcgaattgg ttgagaaaaa gaccaatcag agcaaaaata agaagagaca
     5281 gcaaactctt tctatccttt aaaaagacca gtattttatc ctttgcagta gcataagcca
     5341 agagcatgag aattccacca gcgattaaaa gacgcaagtt agtcaagacc agagccgaaa
     5401 ttccgtgtgc cattaggtat tggccactcg ttcctgacaa cccccaagca ataccagcca
     5461 caactgttag taaagtccct tttaaactat ttgacatgtc tctccttact cttctttgcg
     5521 aatcaagttc atcacttgat tgcggtctaa tatccattga gctctctcgt ccttttcata
     5581 ggtacgattc gataggaaaa tagtggcttc ttgcttctga cgattccaca tgataaaggt
     5641 acctgtatag cccgtatggt ctagccaatc tccttccaaa ttccatgcta aagaacgttc
     5701 cttgtcatcc aaaggagaaa aattttgatt taagtctctt gcaaaatcat ctgctaaata
     5761 gtgttctaaa aagatttgta aatcctttat agtcgaaaat aaaccagcac tcccagcatg
     5821 tctacccagg aaacgagcct tgggatcatg cactatgcct gcctctacac ctctaactgt
     5881 tggaacagca agctcaactg gcccaaactt agtttccgtc attccccaag gtttccagac
     5941 ttgatccttt aaaatcacat ccaaatcttg attaaaaatt ctttccaaaa taaagcccaa
     6001 cagcaaaaaa tggacatccg aataaagaaa ggctggctga cttcgtctgt tgagatgaaa
     6061 catcgcttcc tttaattcag gggctgttaa aagatcacga ttaggaataa aaggatcaag
     6121 gtctgttgca tgagtcaaga gctggcgaat agtgatgtct ggataatcac tctcaggtaa
     6181 aaaatctatt accagtctat caatatctaa ttgacctatt tcccacaaga aggtacaaac
     6241 tgtgccaacc ccaacaacct tgctgacact agctaggtca taaactagtc ctgcctcagt
     6301 ctgcaagcca tgctctgggg cactctggcc taaatagaac tctgtccatt gattgtcctt
     6361 aaaatacgca aaagaggctc cgggataaat tcctgcctcg atttgttctt ctattttttt
     6421 aataatcttg gtccacttca tacttcttca aaccacaatt caacattatt tctatcttta
     6481 ccaaggaaga atttttcaga cttaggaata aaatattcag tagactcaaa cttctggcga
     6541 agacttgcta tgtctaattc attgaccaag aacttgagca tagataagtc ccaggtaacc
     6601 gtattgtcca cagtcaaatc ctgcccctga gctggaataa aatcaaggga tgccccaatt
     6661 tcagatgatt ccaagaaact ttcgatatca gttgggagat gtaattccat agaaatctca
     6721 aatttactta aagaaattga tgccaaatct gtttgaaatt caggcttttc tcctacttct
     6781 actagacttg ctctgtcatc ttccgcatga atcaaaatca aatcatcttc tggtgagaaa
     6841 atttcaaaag cgtagccatt ttgaccttta tataatcgat gaatcgaatc tgttttagat
     6901 aagattcctt caatttctaa gggattttcc accttgacaa tcaatctagc tagttttttt
     6961 cttccctcta ccttacgagt acgcatactg ggagcttctt ctaaaaccag cttttcaaga
     7021 cccgtttggt cacctagtga cagaaaggcc gattcttcta acagggcctt cattccaagg
     7081 gtttcaatat aaaatgtttc atttaatttt ctattattag cttttaaagt aggaataatc
     7141 cgtacaatct gatttacatt cataaattcc tccgtcactt tttattttaa aggattttag
     7201 aattttttac aagatattat gctcaaattt aacattattt agctatttag taaagatagt
     7261 ctttcaaggc ttttgacaaa gcctgataac cagcaatact gaggtgcagt ccatcagttg
     7321 tatattcttt tttgagttgg cctgcttggt ctgtcaaaca atcaaatact ggcacaaatt
     7381 ccacctgcat ataggcagat gcaagctctt gataggcttg attccaattc tgaatttttt
     7441 cattcgagcg gatatagact gcctgctggt acttctctcc ctcattgaca ggcaaaatgg
     7501 aaagcaattt aatctctgtc aatggataat cgcgagcaac ggattgaatg atagcttcga
     7561 gattattgag agcctcattc acaggaacat cctttccgat atcatttgtc ccaatcagaa
     7621 gaaaaatttt atctactgct ccaccatata gatgagcatc aaggttctct agtaacagtc
     7681 ctgtctgata gccacgaatt cctcgattga caatcgtctt tgaagtccca aatagctcct
     7741 gtagaggata atactcgaca atggaatccc caataaaaag aatgtttggt tctacaacag
     7801 aaatgtgatt taggtgacga tatttggttt gaattttttc ttgttccttt aggagccaat
     7861 tttctaataa ctgtactgcc acttcattct cctttttcta accagttttc caagacttga
     7921 taaactcctc cttggctgtt ggctggtgct agagcagtcg ccacagcttt ggctttctca
     7981 tcagcatttt ccatcgcata ggcaattcca gccatttcaa gcatttcaac atcattttca
     8041 ctatcaccaa aagccatgat ttcttgggat ttcaagtccc agcgcttgag taattcctcc
     8101 aagccccatg ctttatgaat cccagcttgg aggatatcaa tgcaaccata gccactggat
     8161 acagctcgga cacggccatc aaagagagca ttgatttctt ccaaaaccga actcaaacgt
     8221 tcctcaccaa caaccatgct catcttgagc acaccaccaa agaggtcaga tgttaattca
     8281 tccacaaatt gcatccgttg gtagaatttt tcaatcattt ctggagtcat aaaactttca
     8341 agatctgtaa aaatcgtacc ttccttgaca aaatcaccct tcatccccgt tacaacaaac
     8401 tggtcctgac acgctcgacc cttgaaatga gccaaagcct tgttgacaat ggcgtcatcc
     8461 catgtctgag cctgaatcaa ttcattgttt tcaaaaatac gagcgccatt agcaacaacc
     8521 agaaccactc gatccaccaa gggactcagt agttgtctca tgcggtgaat ttcattgccc
     8581 gtcgcaatga caaaacgaat gcccctttga tccaactgat ctaaaatctt ttccaatcgt
     8641 gggagatcaa gctgacctct agcatccagc aaggtcccat ccatatctgt cgcaattacc
     8701 ttaatcatca tttactttcc tctggattca agctaatacc acttcaatcc cacatgttca
     8761 ttcatttctt tataggctgt atcaattcgt tccttagtcg cttcatctaa ctggtcacga
     8821 tgactgccat cctctataaa atcttccaaa atataagttt ggtaatgata caagggatat
     8881 ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg agctttagct
     8941 gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag aacaccacgt
     9001 tcagtccact tagcattctc ttcatctccc atagattcaa tccgttgatt ggaaatgaag
     9061 ttccccattt aatagataat gagtttctta tctccatctt tttcaaccgt ttcagatggt
     9121 tcaacaacgt gaggatgccc tccaaagata atatccgctc cccaatcgat catcttgtga
     9181 taaagagctt tttgttcttc agttagttcc aatcgatact caacacccat ctgaagcatg
     9241 ataatggtga tatctgcttc cttctctgcc cgttcaattt caaccttcat cttatcttcg
     9301 tttaaatctg aaagataacg attatagtct tcctgagaaa tatactgctc aattccattg
     9361 aaaccatagg aataggctaa caatgcaacc ttgataccat tcacttcctt aatgaccagc
     9421 ggagcctgat cacgtggttc gtgcgtataa actccgattg gagtgattcc agctttctca
     9481 ataatatcgg ccgttgaaat aactccctca atttgcgaat ccaaaatatg attatgagct
     9541 aaatccagca catgataacc tgcctcctta atagcatcca taacttcagc aggagcatta
     9601 aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa atcaccaata
     9661 gctaaatctg ctgcttagtc cccttgcttg agccaaggag tcacatactc aaaattttca
     9721 tgaaagtcat aggtaccgtc ttctttttta gctgaaaaga aaagtccatc gtggtagagc
     9781 aaatccccgt gggccataat tctggcagtt ttttcctctt cctactcctg agacttttgc
     9841 ttagtcccct cttgagaaat agtatctcgt ttttgactag tcaagggatt ccttggaagc
     9901 tttcaaacaa caagaccaag cccattgata aatcaactgc tagcaagagt atcgccacaa
     9961 atcccttatt gctccacttg cgataactcc taaaaagttt accaagccct tcataaaacg
    10021 aaaagctaaa ccaccctgat ttcgattttg tcttctttgt atcttcattc tccctacttt
    10081 cttatgcaag ccttttcttt ttattatatc acagataagt atttctttca caattgaatt
    10141 gaacttccca tctattttct ataaatccta aatgccataa tgctttcaat tcttgtcatt
    10201 ttgtgatatc atgtagagga aatgaactaa tccacagtgg cttattccaa gtataccact
    10261 tgggctttgg cagtagctaa ctgcactaaa cataatataa ggagagaaaa tgtctgcaat
    10321 agaacgtatt acaaaagctg ctcacttaat tgatatgaac gatattatcc gtgaagggaa
    10381 tcctactcta cgcgcgattg ctgaggaagt cactttcccc ctatctgacc aggaaatcat
    10441 cctaggcgaa aagatgatgc aattccttaa acattcccaa gatcctgtca tggctgaaaa
    10501 aatgggactc cgcggtggtg ttggactggc tgctccccag ttagatatct caaaacgcat
    10561 tatcgctgtt ttggtaccta atattgttga agaaggcgaa actccacagg aagcctacga
    10621 tttggaagcc attatgtaca atccaaaaat cgtctctcac tctgttcaag atgctgctct
    10681 tggcgaagga gaaggttgcc tgtctgttga ccgtaacgtg cctggctatg ttgttcgcca
    10741 tgcccgcgtt actgttgact actttgacaa agatggagaa aaacaccgta tcaaactcaa
    10801 aggctacaac tccattgttg ttcagcatga aattgaccac attaacggta tcatgtttta
    10861 cgatcgcatc aatgaaaaag acccatttgc agttaaagat ggtttactga ttcttgaata
    10921 aagaaaatcc cgttgcaaga cggggttttg tgttataata gaggcatgaa aacaaatgat
    10981 attgtctatg gcgtccacgc cgttaccgaa gccctccttg caaatacagg aaacaaactc
    11041 tacctccaag aagatctccg aggtaagaat gttgagaaag tcaaggaact agctacagaa
    11101 aagaaggtgt ccatttcttg gacatcaaaa aaatctctct ctgagatgac tgaaggtgct
    11161 gttcatcaag gttttgttct acgagtgtct gaatttgcct atagcgagct agattacatc
    11221 cttgcaaaaa cacgccaaga agaaaatcca cttctattga ttctagatgg tctaaccgat
    11281 ccccataatc tgggttctat cttgcgaaca gccgatgcga ccaatgtttc aggtgtcatc
    11341 attcccaagc accgtgctgt cggagtaact cctgtcgttg ccaaaacagc cacaggtgct
    11401 attgaacacg ttccaattgc ccgagtgacc aacctcagtc aaaccttaga taaactcaag
    11461 gatgaaggct tctggacctt tggaacggat atgaacggta ctccttgcca caagtggaat
    11521 acaaaaggga aaatcgccct catcatcgga aatgaaggaa aaggcatctc tagcaacatc
    11581 aaaaaacagg tcgatgaaat gattaccatt ccgatgaatg gacatgttca aagcctcaat
    11641 gccagtgttg ctgcagccat tctcatgtac gaagtttttc taaatagact ataaaaaagt
    11701 ttccagtcat ctgattggaa acttttttat gattaactat gttctgtgat aaacttgtag
    11761 gcttcttgac cagcgatagc tccatctcca actgctgttg ttacttggcg aaggtctttc
    11821 aagcgaacat ctccaactgc aaagataccg tcgactgcag tcttcatgtg gctatctgtc
    11881 acaatccagc ctgcctgatc ttggatattc aattctttaa caaaatcgct aagagggtcc
    11941 aaaccaacat agataaagac accaccgaag gcttgctctg tcacttgacc tgttttcaca
    12001 ttttcaaaaa cgacagattc tactctgttt tcacccttga tttccttcac tacagaatcc
    12061 cagataaagc tgattttttc attcgcaaag gcgcgatctt gtaaaacctt ttgggcacga
    12121 agttggtcac gacggtgaac aatggtaaca gtcttagcaa aacgagtcaa gaagagggct
    12181 tcttcaacag ctgaatctcc accaccaact accaataaat cttggtcacg gaagaaagca
    12241 ccatcacaca cagcacagta agaaacacca cgactgttca gttcttcttc tccaggcact
    12301 cccaaaggac ggtgtttaga accagttgct acgataactg tacgtgtttc atatgtttgg
    12361 tcatcagtca tcactttctt aaaatcacca tggtcttcga cattttcaac ataaccataa
    12421 atgtgctcaa caccaagatt ttcaagtggt tcaaacatct tttcagccaa ttcaggtcca
    12481 ctaatattag catatcctgg gtaattttcg atatcagatg tattattcat ctgaccacct
    12541 ggcagaccac cttcaatcaa agctactttt agattgcttc gagcagcata caaggccgca
    12601 gtcatccctg caggtccagc accgataata atagtatcgt acatatagat tccttctttc
    12661 ttgttgtaac tatctttatt ctaactcttt cttgtcaatc acgcaaggat tatgccttga
    12721 aaacaagaat taaactcata accgcaaagg ataatactgg gacaaccaag actccaatca
    12781 taatcatatc atagagatgg gcttggcgag ccccggctgt cttatcaact cccgacatgg
    12841 ctctcagacc aatccaaatc cctaaaaaaa tcagaacaag gatggtggtc aagatcaaac
    12901 tctcgaaata taaagaaaat agttgcagta gcatgatttc tctcatttct atctttttta
    12961 aagagtaaac tcagctagtc caactaactg agttttcctt tatctattat atcaaatata
    13021 agtccgtttg taactagcaa agaattcttt tgtccgctct tctttagggg tgtggataat
    13081 ctcatccgga gttccagact cgatgatttt ccccttatct aagaagagaa ttttatccgc
    13141 aacttgggct acaaaggaca tgtcatgact gaccaaaatc atggtctgac ctgacttagc
    13201 agcatctgca atagactttt ctacttcacc gaccaattct gggtcaaggg ctgaagttgg
    13261 ttcgtctaag agcaaaacat ctggtttcat agcaagcgca cgcgctaggg caacccgttg
    13321 cttctgtcca cctgataaat ggcgaggata atggttttca cggtccgaaa gcccaacctt
    13381 agccaactct tccttggcaa tcttagtcgc ttcttggtca gataatttct tgacaacaac
    13441 caagccttct ttcacattat caagtgctgt tcggcgttca aataaattaa actgttggaa
    13501 aaccatagac aacttacggc gtagggcaag gatttcttct tgagtgattt tagaaaaatc
    13561 aactgaaaaa ccatcaatct gaatagagcc actgtcaggt gtttcaagat aattgagact
    13621 gcgaagaaag gttgattttc cagctcctga agaaccaatc aaggctacaa cttccccttt
    13681 ttgaatatcc aagttcagat gatccaagac agtctgtcct gaaaaggatt tgcttaaatt
    13741 cgaaatctta atcattaacg aaggtctcct ttcacatctg tttgcactgt atcaggtgca
    13801 gaaatagcca tttttctctc gatgaaacga ccgaggcttt caattccgat attgactacc
    13861 caataaacaa gggcaacgga gatgaagcgt tcaaaatagc gataatcagc tccacctaga
    13921 atctgagctt gggcaaagac ttccacaaca cccgcactaa aagctagaga tgttcccttg
    13981 gtcaaaccga tgagggaatt aatcaaggtt ggagtagcta ccaccgctgc attaggaata
    14041 atcactcgtc gataaacttg cgctcgggtc atacccagac tgcgcgccgc ctcaatctca
    14101 ccaggattaa ctgagagaat ggctgcacga atggtttcac tagcataagc tgcctcatta
    14161 aaggcaaaag cgacaatcgc aaaagctgca gctggaatcg cattgatatt gagaccagtt
    14221 ccccattgct gattgagggc tttcaaagcc aaagggattc cgtagtaggt caacatgagt
    14281 tgcaccaaaa tcggtgtccc ttttaagaaa ctaacaaaga aggcctgcaa gggatataaa
    14341 atcttgacac gattgatctt cacaatggca aaaagaagcg ccaaaaccaa gccaaaaagg
    14401 gcaccaccaa ttgtcaacat aattgttgtt ggaagttgtt ggacaattct agggattcca
    14461 tcaaagaccg aacgtaggct aaacagcttg ccatccggaa tcaattgcat caagttttgg
    14521 taccaatctg atgctaaaat cgttgtaaca ttcataaaaa catcctcttc tgataatgat
    14581 tcattctacc atgcttctgc cgtcaatgaa attatttgct acggtcagat acagactttg
    14641 tctacctact agtttatcat actttgaaac agggaagtta tatattttat ctatcaaaga
    14701 gatagataaa aactatttca gtcccagttc ttcataagat tttcgataac cgatttgctt
    14761 aacagttcca ttttctacta aaatgggccg ttttaacaac ataccgtcac ttgctagcaa
    14821 ctcagccgct tcttggtttg acaaacttcc taccttatct tttagcccta attcacggta
    14881 tttgatacca ctggtgttga aaaattgctt caattcaaat cctgaggttt ctagccaatt
    14941 caaaatgact tcttggctag gtgtttcttc cacgatatgg acggctttat aatccacact
    15001 taattgattt aattcttgtt ttgctttttt acaagttgaa catttggggt attcgataaa
    15061 ttctaacatg tgcttctcct tctatttgtt tatatcttta aaatctacgc cttcatgctc
    15121 caagagccaa gctttctttt ccactcctgc agcatagcct gtcagacgct tgcctgctcc
    15181 caacacacga tgacaaggta ctaggataga ccaaggattg cgtcccactg ctccaccaat
    15241 tgcttgagca gaagccactt gcaggtcttg agcaatttgt ccataggtca ctgtctgacc
    15301 ataaggaatg ccctgtaaat agtcccaaac tctcttttca aaattcgttc cgattggcgc
    15361 caagagcaag ttggataaat cctgaggctt gcctttaaag taatcatcta agcaagcaat
    15421 aactgggtct aaaataggat gactaacaac ttcttctatc gtttcatctc ctagtcccct
    15481 ctcaaaatgc ttctgctcct gaacccaaat tccatacaaa taatggtcat cagctacaag
    15541 taatagaata ccaattggtg aggagtagag tatttttaca tacttctttt gcattatttc
    15601 tttaattttt gataggccaa gcgttcgcca tctactggca ccttaccaac ttgtttaaaa
    15661 ccaagttttt caaaaatatg ttgcataacc ttgttttcag catgcgtatc tgagcgaaaa
    15721 tcaagataat caaaaccttc aatcaagccc tctaagaagg tttgagcaac tccttttccc
    15781 tgcacatctg ctgccacagc aatacggtga aagactagat actctgactc tccagcttgc
    15841 cagtttccct cataaatagc ttcataggcc tcctctggac tcttggtcac agcagcatag
    15901 gctagcagtt ctccctcttc caaggctacg taggcttgcc ctgagatgat atcatcgata
    15961 ataacatctg catttggata gccattttgc cactggtcac taccagcatc tgctaaacat
    16021 tttttggcat cctccatcac ctgcatgatg gcatctactt catttggaaa agctaaacga
    16081 atttccatct tttttcctca tttctgacta gtttctccca tcatagcata atttaagtat
    16141 ttctacaagc agttaaaact tgactttgtg tttttgttat tttataatct agttatcaaa
    16201 actatataaa aaggagttga aaatgaaaac taccttttcc tacccaaaat gggcagaaat
    16261 tccaaacatt gacctctatc tggaccaggt tttgctctat gtcaatcagg tctgcgcccc
    16321 tatctctcct aataaagaca agggcctaac agcatctatg atcaacaatt atgtgaaaaa
    16381 tggttacctg acaaagcctg acaaaaaaaa ataccaacgc caacagattg cccgtttgat
    16441 tgctatcaca accctcaagt ctgtattttc tattcaagaa atagcccaga cacttaatac
    16501 tctacaaact caagcaagtt cagaccaact ctacgatgct tttgtggact acatgaacca
    16561 aggaattgac ccagctaacc ctattatcca aaccagctgc caaaccgtta aactctatca
    16621 tcaaactcta gacttaatcg atcatactca agaggaggta atccaatgaa cactagtctt
    16681 aaactcagca aacaactcag ttttggagag gagattgcta atagcgtgac ccatgctgtg
    16741 ggtgcagtca tcatgcttat cttgctgcct atttcatcca tctatagtta tgaagcacac
    16801 ggatttttat cctctatcgg cgtttccatt ttcgtcatca gtctctttct catgttccta
    16861 tcatccacca tttatcactc tatggcctat ggttcgaccc acaaatatgt tttgcgaatc
    16921 attgaccatt ctatgattta cgttgccatt gccggctcat acacgcccgt tgtcttgacc
    16981 ttgatgaata actggtttgg ctatctgatt attgtcatcc aatggggaac gaccatcttt
    17041 ggtattctct ataaaatctt tgctaaaaat gtcaatgaga aatttagcct tgctctttac
    17101 ctgattatgg gctggttggt tctggctatc attcctgcca ttatcagtca aacgacaccc
    17161 gttttctgga gtctcatggt aactggcgga ctctgttata cagttggagc tggattttat
    17221 gccaagaaaa aaccttattt ccacatgatt tggcatctct ttatcctagc tgcgtccgta
    17281 cttcaataca tcgctattgt ttattacatg taaaaaagtt gagaaattca atctcaactt
    17341 ttttctttac acatattgat aaagtactgg tgcaagcgca catcatcagt caattctgga
    17401 tgaaaagaac ttaccaacat atttttttct tgggctgcaa caatttgatt gttcactgtt
    17461 gctagaattt ctacaccctc accaacacta ctgataatcg gaccacggat aaaggtcatt
    17521 ggaatcttgc caactccctt acattctgct tccgtgtaga aacttcctaa ttggcgccca
    17581 taagcattac gctcgaccac catatccata gttcctagat aactctcttt ctgagaagtg
    17641 atttccttag ccagcaaaat taagcccgca caggtcccaa acactggtaa gccagataga
    17701 atggcttctc ggatgagaag tagcatgttc tggtcacgta agagcttgcc catggttgta
    17761 gactcaccac caggcaaaat caaacccgac aagtcactct gatcttgctg aaaatcatct
    17821 agatttctga gttctacact ctcgacacct aattgatcta gcacttttgc atgttctgca
    17881 aaggcccctt gcaaggccaa tattccgatt ttcatctatt ttcctcgttc agccatgaga
    17941 atttggattt cattttcatt aataccaacc atggcttctc ctaaatcttc agagatttga
    18001 gctaggattt gaggattacg gaagttagtc acagccttaa caatggcact cgctcgttta
    18061 acaggatctc ctgacttgaa aatacctgaa ccgacaaaga ccccctctgc ccctagttgc
    18121 atcattaacg cagcatctgc tggcgttgca acacctccag cagcgaaatt tacaactggc
    18181 aattttccat gttcatgaac atattggacc aattctacag ggacttgcaa atccttggca
    18241 gcaacataaa gctcgtcctc acgtaagttt tgaatgcggc gaatttcctg attcatcata
    18301 cgcatatgac gaacagcttg gacgatatcc cctgtccctg gttctccttt ggtacgaatc
    18361 atggaagcac cttcagcgat acgacgcaag gcttcaccca aatccttagc accacagaca
    18421 aaaggaactt ggaattcttt cttgtccaca tggaaacggt catcagctgg agatagaact
    18481 tcactctcgt cgatataatc aatttcaata gcctctaaaa tctgagcttc aacaaaatgc
    18541 ccgattctga ccttagccat tactggaata ctaaccgctt cttggatttc cttaatcatc
    18601 tttgggtcgc tcatgcggga aactcctcca gctgcacgaa tatcagccgg aattcgttcc
    18661 aaggccatca cagctgccgc accagcagct tctgcgatac gagcctgttc aggattctgc
    18721 acatccataa taacaccacc cttgagcatc tgtgccaagt ttttatttag ttcataacga
    18781 ttttcagtca tttgttttat cctcatcatt tgtattggta gctacctttt cattttaagt
    18841 cagaacagaa tgaagcacaa gataaaaaaa ctataattgt atggggacaa attagctaaa
    18901 aactatctga ccttaactta aggccagata gctcattcat ttttattttt cagccgtaag
    18961 ggcagccatt gtgatgtagt tgtatggttt gttgaagtgt ggcaagaaga agaggtctgt
    19021 caatgccaat ttatcaattg tcacatgctc ttggatagca agtgagaaca tgtggattcc
    19081 catgctaatt gcaatatcat gtgaaaccat ttgggcacca agaatttcac ggctatcttt
    19141 gtcaaagaca atcttaattg ctacttcatg gttgtcatgt ttcatgaatt ctggtttttg
    19201 aagatcgtta aagcctgttt cagttgcgtt gtaaccagca gctttcgctt tttcaagagt
    19261 caaaccagtt gaaaccatgt gaagaccgta gattgagata ccatttgaac cttgaacacc
    19321 gattccttcc aattcatgtc cacaagcatt gtaggcacca acgataccag tgcgaacagc
    19381 atttgaagca agagcgatat agcttgtatc tttacgagca ttgtcataaa cagtcgcaca
    19441 gtcaccaaca gcgtaaacgc ctgggataga tgtttcttgt ttcttgtcta caaggaaggc
    19501 accgttgcgg aagagttcga tcttaccatc tgcaagggct gtgtttggac ggaaaccaac
    19561 tgcaaggata accatatcca cgtcaaagct ttctttgtca gtaatcaagc gttcaacttt
    19621 accgtcacct tcgattgctt taacagtttg acctagagcc aagcggatgt tgtgatcttc
    19681 caagttcttc gccatcattt gtgtgaagtc tttgtcatag taaccgttca agacagtatc
    19741 aacgatatca acaaggacaa cttcttttcc aagacgttca aaggcttcag caagttcaac
    19801 accgatgtaa ccaccaccaa caacggcgat acggtcgagg tgttggctct tgtcagaaag
    19861 tttattgata acttcttcag cattttggta caatttcacg aattgtacgt tttcaagagt
    19921 tgctttaaat tcgcggtttc ctttaacaat ttcaacacct tcgattggtg gcaagattgg
    19981 tgtagaacct gtagcgaaaa tcaatttttc gtatgattct ttgtgctctt ttccttcaac
    20041 ttccgctgta actactttgt tatcatagtc gattgaaaga acaggtgagt tcatgtaaac
    20101 tttagcacct ttagcttcca atttttcttt atcagaatag aacaagcctt cagcaccgtc
    20161 aatttgttca ccaatccaaa gagccattcc acatcctagg aaagagatgt tagagttttg
    20221 gtcaaataca acaatttcgt tctcatttcc aaaattatcc aacatggtat tgatacatgc
    20281 tgtaccagcg tggttagcac cgactacaac gattttactc ataaaaattt cctgccttca
    20341 ttctaaattt accttactag tataccattt accgttagcg tttacaaggg gggtactatt
    20401 atttcagcat tttttatcaa aaataattcc ccatttatcc tgttttcaag cctcatgaat
    20461 tttatgagca tatttcttga ctttttccaa attttgtttg tgaaaattct aactttatgg
    20521 tattataata acatgaaaat gaaatgtaag ggcttcaatt tagatgatta tttgatatta
    20581 caacttattt tctataagag aaaggagttg gtagcttgac tcttcgttca cattctgaac
    20641 ggctaatggg gactactatc actatttcat tagtggatga gcaagccgat atctttctcc
    20701 aaaaatcctt tgacttgctc aaagagcttg aataccgctt caacgccaat agtcaagaat
    20761 ctgagttgat ggaaatcaat tatcaagcag gaatatcccc cgtcacggtt catccagacc
    20821 tgtttgagct gatttcactt gggttagagc atagcctagc gctctctagc cacctcaata
    20881 tcagcattgg tcccttaatt caaacctggc gtatcggttt ttcagatgcc aaggtcgccc
    20941 agcctcaaga aattgaatcg gtgctgcctc taatcaatcc tcatggtatc gagttagatt
    21001 cttctacttc tactgtgttt ttaaaacaga aaggaatgaa gatcgatcta ggttgtttag
    21061 ccaaaggata cagtgcggat aaggttgccc aatttcttag gaaagagggg gtgacttctg
    21121 ccttgatcaa tctgggaggg aatatcctga ccattggaaa aaatcaggca agaggggata
    21181 acccatggca aatcgggatt caagacccag ccaatccgag gggaaatcat ttaatgacca
    21241 tccctgttgt caataaatct gtcgtgactt caggcattta tgaacgtcac ctgaccgtcg
    21301 atggacaaga ttaccatcac atttttgaca gtcaaacagg atatcctgtt gaaacggaac
    21361 tagcgagtct aacaatcatc tctgataaat cagtcgatgg cgaaatctgg acgactcgac
    21421 tctttggaga aagacctgct tctatcttct ggcaagtcga aagtttggag ggcatcgaag
    21481 tcatcctcat cgataaagaa ggccacctaa gctgttcttc aggaattcct actctataga
    21541 gacaactgtt tcaatggaat tttaaaattg tattatgtaa aaagagaaag gaaatctcat
    21601 gttaaaactt attgctattg ttggaacaaa ttcaaaacgt tctacaaacc gtcaattgct
    21661 tcaatacatg caaaaacact ttgctgacaa agctgaaatt gagcttgttg aaatcaaggc
    21721 cattcctgtc ttcaacaaac cagctgacaa gcaagtacct gctgaaatat tggaaattgc
    21781 tgctaaaatc gaagaggcag atggcgttat tatcggtact cctgagtatg atcactctat
    21841 tccagctgtt ttgatgagcg ctcttgcttg gttgtcttat ggtatttacc cacttttgaa
    21901 caaaccaatc atgattacag gtgcttctta cggtacgctt ggttcatctc gtgcccaatt
    21961 gcaacttcgt caaatcttga atgctcctga aatcaaggca aatgttcttc cagatgaatt
    22021 cttgctctca cactctcttc aagcatttaa cccaagtggc gacttggttg accttgatgt
    22081 tatcaagaaa ttggatgcca tctttgatga cttccgtatc tttgtaaaaa tcacagaaaa
    22141 attacgtaat gcacaagaat tacttcgcaa agatgctgaa gactttgact gggaaaattt
    22201 gtaagatagg agaccaaaaa gaatgaaatt tgttggactt gttggatcaa actacgatca
    22261 atcatataac cgtaagctct tggaatttat ccgtcgctac ttcaaactca aatttgaatt
    22321 agaagttctt gaaattgacg aagttccaat gtttaaccaa gatgaaaaat gggatgaaag
    22381 tttccaatta cgttatttat ataacaaaat tactcgtgca gatggtgtta tcattgctac
    22441 tcctgagcat aaccacacta tctcagcttc actcaaatct gtacttgaat ggctttcata
    22501 cgaagttcat ccatttgaaa acaagcctgt tatgattgtg ggtgcatcat actatgacca
    22561 aggaacatca cgtgctcaag ttcaccttcg taaaatcctt gatgctccag gtgttaatgc
    22621 ctacacgctt ccaggtaacg aattccttct tggtaaagct aaggaagctt ttgataacaa
    22681 cggaaatatc accaacgaag gcactgttaa attccttgaa acttgcttag ataactttgt
    22741 taaatacgta ggagtcgttt cgaaattgaa aaaaccaaaa ccaatcgaat cagaagactt
    22801 ggattgtgga aaaccaatcg ctacaaccat cacagaagtt gaccctgacg atccagaatg
    22861 ggtagaaaaa gttgcagcaa tcactggtgc tgtttctggt gatacctatg tcaaattgga
    22921 ccacggtatc ctaacagtta accaaattga tatgttcttg aaagctatgc catttgaatt
    22981 gacatacgct gacgacaaca accaattcct ctactacaac aacgctcacc aagatccaga
    23041 cactatgttc gctaaacgtg taccacctca atcaggtagc cgtatgtcga ctgttcatgg
    23101 ttctcttcca ccagcacgta tgaagaacgt agagtgggtt atcggaacac ttcgcaacgg
    23161 aaaccaagaa tacgtccgta cgatcgttcc aggttctcct gcaggtgtta tcaacactca
    23221 caactaccaa gcaatgtact atcctgatgg atcatacgct ggtatcaatg aaatcgtctt
    23281 taacttccaa ccatggcttg actggtacct aaaagagact ggtcaacgtt tggtgggtgg
    23341 tagcggacca tttgctcctg ctgccggagg tcatggagac gccgatgcta cttctggcgc
    23401 ttctgattca ggggatgctg gaggccacgg tggtgacgca gacgctacag ccggtgcaag
    23461 ttactaatga tagtttttag gaaccattat ggttcctttt tagcgaaaaa agactagcaa
    23521 acacaaactg ctagtctctt tgttatcgtt tcatctatct ttgttaaaag aaagaataca
    23581 tattatgaat ttggatcatc aagactacgg ccatgtaatc ctttttcacg ttgtacttgg
    23641 cgtagttttt ctggtgtaac atcgtttcct tcttcgtcca caattttgat tccttcaatg
    23701 tggtgacgaa cagcgcggcg ataaccttcg atgtactcct cacgtagttt ggcttgttcc
    23761 actttttctt ctggtgttaa gccttctgtt tttttctttt tagcaagctc attgatacga
    23821 gcaatttttt tcggatccat ttcttctcct ctgtaataag ataaagtccg tttatcaaat
    23881 tttatttagt gttaatttgg ttaaaacaag caaacttagc tgctttcttg gttagttgtg
    23941 acaagattgc caaacgattt tgacggacag cctgatcttc agccattacc atagtatttt
    24001 caaagaaagc atcaatgact gggctaagcg caaagagttg tttcaattgc tgacttgcag
    24061 gtcctgataa aacgagtgtt tctactgctt ctgccaaagc tttttcttgg tcattctcaa
    24121 atagtgctga atcaactgta gcaacccctt ctgccttctc agccaggtta aaggcacgag
    24181 aaagtgattc aacagatggt ttaaaatctt cttccttgct tacttctacg agagcacttg
    24241 ctgcttccaa catatctgcc acaacaaagt ttgaacctgc aagaactgct tccttgatat
    24301 cttttggagt agagcccatc atcttatcaa cacgagcctt gataaagtcc ataacctctg
    24361 ctttattttc ataagtcaaa ctgtcaaatt tcaatgcata aaggctatca atcagctcat
    24421 ccatagcaat gtgccaacca aaggcatcca agatacgaac cacaccttga gttgcacgac
    24481 gaagggcata agggtcatta gaacctgatg gaatcaatcc tactgagaag aaactcaaaa
    24541 tcgtatccaa tttgtctgca atggctagaa ctgcaccgac cttgctctct ggaagttctc
    24601 cttcagctga tgtaggcatg tagtgttcac gaatagcagc tgccaccgct ggagtttcac
    24661 cagcaagaag ggtgtatttt tcacccataa ttccttggag ttcgtcaaat tcaccaacca
    24721 tacctgtcaa caagtcaaac ttgtaaatgg ctgctgcacg agcaaggtca actgtttcat
    24781 ccactgacaa accagctttt tctgccaaaa gtacagtgat ttgacccgta cgaatcatgt
    24841 gttcacgaag ggaaccaatc ttctcatgga aggtgacatt gtttaatttt tcaacaagat
    24901 ctgaaatcac caatttttgg tcttcacgcc agaagaattc tccgtcttcc aagcgggcta
    24961 ccaagacttt ttcatttcct ttgatgacat ttttcaaacg ctctgcgttt ccgttacgaa
    25021 cagaaatgaa gtttggcaag agttttccat cttgatcacg aacaacaaag taacgctggt
    25081 gttccttcat agaagtcacc aagacttctt ctggaacttc aaggtattta gcatcaaaac
    25141 ttcccatgaa ggcagttggg tattcaacca aattcaagac ttcattcagc aaatccgcat
    25201 caatttcgat acgtacacca tgttttgcct caattttctt gatttggtca acaatcattt
    25261 gctcacgttc acatggatct gcgattacaa actgcttacg aaggtcttct tcatagctca
    25321 atgctgactg aattttggtt tctttcccca agaaacgatg gccacggctc acacgacctc
    25381 ccttgatatc aaggaaatcc aagtcaaact cttgctcatc caagagaaca gttaaagtgt
    25441 gaacagggcg gatgtattca aagctatttc cagcccagtg catgctgaca gggaaagtca
    25501 atgacttcaa gacatctaca acaccaggaa caatggcttc aactgcttga ccaacttctt
    25561 ccttagtgac atagacatat tcttcaccct tgatttcacg gaattcgata tcttcaacag
    25621 tcaagccttt tccacggaca aatccttgag ctgctttggt gaagtttcca tcactatcca
    25681 aggcaatttc ctttgctggt cccttgaaat cttctgtcaa atcagactgt ttgtctgata
    25741 aaccagtcac acgaacagcc aaacgacgtg gtgttgagaa ggtttgaatg gcttcaaaag
    25801 acaggcggtt ttccttgagg aaggctgcca ttttttcgcc tagttgtttt tcacttggtg
    25861 tgacaacata ggctggtaat tcttcaagac cgagttctac taataagttt tttgtcatgt
    25921 tttttctccg aaaagtatct ttttattttc cagtttgcct tatgtgattg gcacgcaagc
    25981 aaccaacttc gttgtttcat tgctaaaatt ggagccaaaa gtctccaatt ttgcctagta
    26041 tccttagttt cactaaggat accttcatca ctgcggcaac tgactcaggg ctcactctgt
    26101 tcgcccattt tcgtaatttg tcactctctt tattctgcgt cttctgctag gagtttagct
    26161 cttgtttctt catccaaaag tgggtaacct aggcgtttgc gttctgcaac aaaggttttg
    26221 gctacaacac gggccaagtt acggatacga gcgatatagc ctgcacgctc tgttacagat
    26281 acagcaccac gcgcgtcaag cagattaaag gtatgtgaac atttgagaac atagtcatag
    26341 gcagggtgta ccaagccttc ttctaatgca cgaccagctt ctttttcaaa cttatcaaag
    26401 ttttcaagca acatttcttg gtccgaaatt tcaaatgaat attttgagtg ctcatactca
    26461 ggctggataa agatttctcc gtattttaca ccatcagccc actcgatatc atagacagag
    26521 tctacttctt gaatgtaaga agccaagcgc tccaaaccat aggtaacttc cgcagtcaca
    26581 gggccagttg ccaatccacc gacttgttgg aaataagtga actgagtgat ttccattccg
    26641 tcaagccaaa cttcccaacc aagaccagct gaaccagttg atgggttttc ccagttgtcc
    26701 tcaacaaaac gaatatcgtg ctccaaagga ttgattccca atttttccaa agactcaagg
    26761 taaagttctt ggatatttga tggagaaggc ttcatgacca cctggaattg gtggtgttgg
    26821 tagagacggt tagggttttc cccataacga ccgtcagcag gacgacgtga tggctctaca
    26881 taagctgcat tccatggctc aggtccgata gcacgaagga aagtgtaagg actcattgtt
    26941 cccgcacctt tttcattatc ataagcctgc ataagcatac aaccttggtc attccaaaat
    27001 tgttgcaaag taaggatgat ttcctggaaa gtcaatttct tagacattgt ttactccttt
    27061 ttatataaat tacttgcgac atgagtctgc ctcgtcgatc atacgaggca agacgagtta
    27121 gtactgcgtc tagctcagca ccctagtgct gagcgtgggg cagtccgact gtaaggaggt
    27181 ctctgccact gatgcagtga aatgttaatt tcttagacat tgtttactcc tttagttttt
    27241 atatttcttt ttcaattaat ttctgctgaa caaaccaatc taacaaggga aaaatttttt
    27301 ctgtatttgc ccataaatga taagaatcta agaccgcttg tacactacct aaattttcta
    27361 ccaacttatc tactgtcaaa aagcttcgcc agtattcata aaaaatactt gcatagttct
    27421 tatcataagt cgaagaacca aaatcattca atgaatgcca accatacttc ttctgaaaaa
    27481 gttctacgag agattgatta caaatttttt ccgcttgaaa ttcctcttct gtaaagaaat
    27541 acttgcgact aatatattca accatcccct cttcgaacca gatataagag tcataaccat
    27601 caaaatcatc taaaaaatgc tccgaccaat gagctaactc atgtcctaca atctgtaaga
    27661 ggaaattttc agataaagaa tgatagtgac tttctattgc ttgagtttgc tcagaacact
    27721 cgtagtccat caattgatac aaatacaact ctttccaaac agctaaatca ggcgccataa
    27781 ccattcgtct attatttgta taggctggaa cagcgctttc cctaataatc tgtgtagcag
    27841 cattaaaatc tgaccaaata acggcttgcg gtaaatcaca gactgcaagt tcattcttta
    27901 aaaaagattg ataatctttc aacttttcag tatttcttac tacaaaatca cgaaatgtag
    27961 ctagttgact atcgtctttt acaagataaa ggttgtccac ggctctcctt tctttcacac
    28021 aatagactga ataagcaaaa gaaccgcgtc cgacaatcct aggttgattt aacctagggg
    28081 cgtcagaaac gcggttccac cctaatttat tacttcattg tttttgaaaa tactacagaa
    28141 agcgccagct cattattttg ccctacttgg ctcccactat cccaagctcg cttgaagaac
    28201 gccataagac tttctttcct gctgactatt atatcaaaaa ttagaataat gtacaattct
    28261 ttttatgatt ttctagaaat ccacatcatc aactcgtgga gcacccgatt gaacagcaat
    28321 cgtttttaag gtttctctct cctcatgact cagttcaatt ccaaagcaat caagattagc
    28381 ttgaatacga gaggttgtga cagattttgg aagtggtaaa aatccttctg ccaagctcca
    28441 ggccaaggct atctgagcaa tcgattttcc gtgatttgct gcgatttctt gcacttgctt
    28501 gctatcaaat agttctccct gaccaaaagg cccccaagct tccaataaaa ttcccttttc
    28561 acgacagtaa gctacgactt gatcttgata cacacctggc gccaagcgaa cttgattgac
    28621 cgcaggaacg atagttgcag tttcaagcaa ggcatccaaa tgatggggaa gaaaattgct
    28681 aacgccgata gcacggattt tcccttcttg atagaggtct tccatcgctc tccaaacttc
    28741 cgcattgcga gttttccatg cgtcattttc tctgagcggt tttgggttcg gccaatgaat
    28801 caaatacaaa tccaaataat ccaagcccag tttttctata gatttttcca aagcttgacg
    28861 agtttgctca taggtttgct gactattcca aagcttggta gttacgaaca tttcttcacg
    28921 tggaactccg ctatctttga ttgcttgacc aacactttct tcattctgat aaatcgccgc
    28981 cgtatcaata tgacgataac cagccttcaa ggcttctaac actgcatgat aggcttcttc
    29041 tccatcctta gccttaaaag ttccaaatcc caatactgga atttctactc cattatttaa
    29101 ttgataggta ttcatatgct tctccttcta ttttattcat caattatagc ataagacatt
    29161 ttttaagtag tactatcttc tgtaaaaaat aagtctgtca aatatctaac agacctattt
    29221 tttcttgctt attcatcacg actatagtac agctcatgag cttttaggcg agtattgagc
    29281 atctctggta tggtcacaaa ggtataacct tgatttttca aatactcaat gacccttggc
    29341 aaggcattga ctgtcggact gtgaatatca tgcatcaaaa cgatagagcc gttagctact
    29401 tgatgctgaa tttctgtcaa aatagatgct tcatttttac tcttccagtc cagactatcc
    29461 acatcccaca tgataaagct caaatccaag ctattgcgaa tatcatctgt aatagcacca
    29521 taaggtggac gcatgagttt agaactagaa cccagcactt tagttagcac atcctcagta
    29581 tcagtaatct gctttttagc ttcatcaaga gagagttgcg agagaatcgg atggctccaa
    29641 ctatggtttc caacaacatg accttcagat tttatcctct tcaccaagtc ctcattccca
    29701 gaaacatttt tcccaagcac aaagaaagtc gctttaatat catatttagc tagggtctct
    29761 aatacctgcg gggtcgttgc tggatttgga ccatcatcaa aggttagagc gacaactttt
    29821 tgatatttct tatcaaagta agattgatac aaggccgcat ctttttcgag taagtacgaa
    29881 gattggataa catcaaagaa agcagatact ggcaaggcta tctcttctaa attttcaagc
    29941 acaggacttg aataaaggat aatctgacta tccttgtaat caaaattcca tgcagacaag
    30001 tcttggtcag agaagctttt tacaatctgc tcactctggt cttgctctat ttttttatcc
    30061 tctatgaagg aggtcaactc ttttatcagc tgttccttag ccttactagc atctgaaaac
    30121 agttggtcaa gtgtaaaagg ttgcccatct tctgttaagt gaacctttcc tagactggtc
    30181 ttttcagtct cttcaacctt aaaagcaact aaatcataga tttgtttggt cacattacga
    30241 ttaacgactc cctttaaacc tgactcttct tgctctgtat agtagaaaac aagattgtcc
    30301 ttactttcta gcttgtcctt aacatcttga tttatcaact ccctaacaga ggaaatcact
    30361 ttctcccctt ggagaggata ataggcaatc acttcggctt gcccctgacg aaaatgctcc
    30421 ttctgatttc cctcactcaa ttgatcatct ttctcttttt tgagcgattc aatcttttgc
    30481 tcaaaacttt tttgctggta gatcttaaaa gcaataaatc cgcctaatag gcaaatagaa
    30541 attgctaaga taccaatcaa agccaataat acatgtctcg ttttcccgtg tctgccacgt
    30601 cctagtctac ttttattcat atttatatca taacaaattt gagcaataat ttcaatgata
    30661 caatttggca atccacaatt cctctagcaa gccaaagcat cttacatcaa ctccaacatc
    30721 cgtatctgta gagattatgt agttttctat ctatggaatt tctagtaaat tgaagctaga
    30781 atagtagacc tatacttcta aaacattgtt agaaatcgat ttgattgtcc tgatcgattt
    30841 gtcctgttct tatttcattt tactataaaa ctatgaaaag ctaggtcatc tgagaagtaa
    30901 ttactatcga caattgcgat agcgtatact ggtgcgattt gtttgtagct ctggtgggta
    30961 tcaccttcac gttggcgaat tttttctagg ttttgattaa cctgactgca cagataagcc
    31021 cataggcgat tgatgaaaaa attctgatga tgaacctgaa tttcgatgat aacttgaatt
    31081 ccattatcta actccgccaa gacgtctata ctagtataga aatcctgcgc tgagtaggac
    31141 atggaaggca agacgtgaat gttacttccc tccaaaatcg tcacattttt tgctggcaag
    31201 tccagcatat cgcggataaa ttgacaaatc attagttggg ctgatacccg gatgtctgag
    31261 aatcctcctt ttcctccttc tattatagca catttttaaa tctaggtttg ctagattcta
    31321 tgcttctatc tatttattcg gaaaagaaag cttttcttca gaagaaagct aatagcttat
    31381 ttgaaatttt ctcctctctg ttggatagtc ccgtctatct atgttataat gaaagaagga
    31441 tttaaaatcg gaaaaggagt atttatgcta aaattaggtg tcatcggaac tggcgctatc
    31501 agccatcatt tcatagaagc agcccatacc agtggagaat accaactggt cgcaatctat
    31561 tctagaaaac tagaaactgc agcaaccttt gcttctcgct atcagaatat ccaactattc
    31621 gatcaattag aagtcttctt caaatcttct tttgatttag tctatatcgc cagtccaaac
    31681 tccttacatt ttgctcaggc aaaagctgcc ttgtctgctg gtaaacatgt tattctcgaa
    31741 aagccagctg tcactcaacc acaagaatgg tttgatttga ttcaaacagc tgaaaaaaat
    31801 aactgtttta tctttgaagc agctcgtaat taccacgaga aagcctttac tactatcaaa
    31861 aactttttag cagataagca agtgttagga gcagatttca actattccaa atactcttcc
    31921 aagatgcctg acttgttggc tggacaaaca ccaaatgtct tttcagaccg ttttgctggt
    31981 ggagccctca tggatttggg gatttatccc ctctatgctg ctgttcgtct ttttggaaaa
    32041 gccaatgacg cgacttacca tgctcaacag cttgacaata gcattgacct aaatggagat
    32101 ggtaccctct tctacccaga ctatcaagtt cacatcaagg ctggaaaaaa catcacttcc
    32161 aatcttcctt gcgagattta tacaacagat ggaaccttga ctctcaacac gattgagcat
    32221 attcgctcag ctatttttac cgaccaccaa ggaaatcaag tccagcttcc tatccaacag
    32281 gatcctcata cgatgactga ggaagtcgct gcatttgcac acatgatcca gcaaccagat
    32341 ctgaatctct accaaacttg gctgtatgat gcaggctctg ttcatgagct actatatacc
    32401 atgcgccaga ctgctggcat tagatttgag gcagaaaaat gaaaaccaaa ctaccgactg
    32461 aatggcaaga actgagtgac caactcggtt tccaagaatt caccctcatt caaactcaac
    32521 tatttgagcc ccttcttgct ggagaaaacc tcctaggagt gagccaaacc ggaactggta
    32581 agaccctagc gtatctccta ccaagtcttc tcagactaca aaagaaaaaa gcccaacaac
    32641 tcttgattct agcaccaaat acggaacttg ctggacagat ttttgatgta tgtaaaacgt
    32701 gggcagaagc tatcggattg actgctcaac tcttcctttc aggttcaagt cagaaacgac
    32761 aaattgaacg ccttaaaaag gggtcagaaa ttctgattgg aactcctggc cgtatctttg
    32821 aactcattaa acttaaaaaa atcaagatga tgaatgtgga aaccatcatc ctggatgaat
    32881 ttgaccaatt actagatgat tctcagattc actttgtaga aaaaatcact cactacgcac
    32941 ctcgtgacca ccaactggtc tacatgagtg ccacgaccaa gtttgaccaa gaaaagattg
    33001 tgccgaatac acgcactatt gatctctctg atcaaaaatt ggacaatatc cagcatttct
    33061 acatgcaggt agaccaacgt catcgagtgg atatgctacg aaaactggct catgtagagg
    33121 atttccgtgg tctagtcttc ttcaatagcc tgtcagacct tgggaatgcc gaggaaaaac
    33181 tacagtatcg tgatatattg gctgtttccc tcgctagtga tgttaatgtt aaatttagaa
    33241 aaatcatctt agaaaagttt aaagataacc aactaaccct gcttcttgca actgaccttt
    33301 tggctcgtgg aattgatatc gatagcctag aatgtgtcgt aaactttgat attcctagag
    33361 atagcgaaac ctacacacac cgtgctggcc gtactggtcg tatgggcaag gaaggttatg
    33421 ttatcactct cgtcactcat ccagaagaaa tcaaaaaact taaaaagttt gcaagtatcc
    33481 gagaaattgt cctaaaaaac caagaactct atatcaaata aagccctatt gctctataca
    33541 catccagtgt atggagcttt tttataagca attcaatttt atactcatct gctttcaaaa
    33601 agcattctag tccatttccg attaacgatg gactttatca cctccttctc cagtccttgt
    33661 atgacatctt ccaaagttcg aaaggcttta ttcttaaatc cacgtttacg aatctctttc
    33721 cacacttgtt caatggggtt catctctggt gtgtatggag gaataaatgc aaagccaata
    33781 ttagtcggaa tctttaaggt acttgattta tgccatatag cattgtccat aacgagtaaa
    33841 aaataatcat atggataagc ttgtgaaagc tcttctaaaa agacgttcat ccactcagta
    33901 ttacatccac cagctattaa gaaaaatgat tcgcttgtat aggcatcaac agctccataa
    33961 caatagcgaa attctcgtat atagtgacta tggacatgtg gacctactcc tattggagac
    34021 caacaagatc ccagtttact gattctaccg aaaccagcct catcttggta catcaagcga
    34081 accttatgaa aacatctact agttttaaag cgctttcttg tcttcttgaa ttgagatttt
    34141 atttttagac gcgacaatgg tttgagcgtc tgctttccta ggatgttctg gacgtggcat
    34201 aatatttcgc caaccatggc gcttcaacag ttgatagaag gcatcacgtg tgtaggaacg
    34261 acctaactcc tttttataag cctgaaataa ggcatcaatt gtaacaaatt ctcctgcctc
    34321 tg
//
LOCUS       DAVLXF010000010        32752 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLXF010000010 DAVLXF010000000
VERSION     DAVLXF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32752
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.010"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(310..2037)
                     /gene="ezrA"
                     /locus_tag="VYO12_000455"
     CDS             complement(310..2037)
                     /gene="ezrA"
                     /locus_tag="VYO12_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1587109.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAESYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2119..4065)
                     /gene="gyrB"
                     /locus_tag="VYO12_000456"
     CDS             complement(2119..4065)
                     /gene="gyrB"
                     /locus_tag="VYO12_000456"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1587110.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4080..4643)
                     /locus_tag="VYO12_000457"
     CDS             complement(4080..4643)
                     /locus_tag="VYO12_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1587111.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTSFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4728..5282)
                     /locus_tag="VYO12_000458"
     CDS             complement(4728..5282)
                     /locus_tag="VYO12_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1587112.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5293..6516)
                     /locus_tag="VYO12_000459"
     CDS             complement(5293..6516)
                     /locus_tag="VYO12_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1587113.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPRLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6553..9003)
                     /locus_tag="VYO12_000460"
     CDS             complement(6553..9003)
                     /locus_tag="VYO12_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1587114.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSCRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVVSLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9203..10132)
                     /locus_tag="VYO12_000461"
     CDS             complement(9203..10132)
                     /locus_tag="VYO12_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1587115.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10272..10559
                     /locus_tag="VYO12_000462"
     CDS             10272..10559
                     /locus_tag="VYO12_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1587116.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10593..11927)
                     /gene="ciaH"
                     /locus_tag="VYO12_000463"
     CDS             complement(10593..11927)
                     /gene="ciaH"
                     /locus_tag="VYO12_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1587117.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11917..12591)
                     /gene="ciaR"
                     /locus_tag="VYO12_000464"
     CDS             complement(11917..12591)
                     /gene="ciaR"
                     /locus_tag="VYO12_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1587118.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12700..15246)
                     /locus_tag="VYO12_000465"
     CDS             complement(12700..15246)
                     /locus_tag="VYO12_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1587119.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15369..15809)
                     /locus_tag="VYO12_000466"
     CDS             complement(15369..15809)
                     /locus_tag="VYO12_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1587120.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15784..16653)
                     /locus_tag="VYO12_000467"
     CDS             complement(15784..16653)
                     /locus_tag="VYO12_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1587121.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLDRILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16631..17131)
                     /locus_tag="VYO12_000468"
     CDS             complement(16631..17131)
                     /locus_tag="VYO12_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1587122.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17142..17840)
                     /locus_tag="VYO12_000469"
     CDS             complement(17142..17840)
                     /locus_tag="VYO12_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1587123.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQEIQEIFEINYMTPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17833..18069)
                     /locus_tag="VYO12_000470"
     CDS             complement(17833..18069)
                     /locus_tag="VYO12_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1587124.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(18169..19095)
                     /locus_tag="VYO12_000471"
     CDS             complement(18169..19095)
                     /locus_tag="VYO12_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1587125.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19170..19985)
                     /locus_tag="VYO12_000472"
     CDS             complement(19170..19985)
                     /locus_tag="VYO12_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587126.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(19989..20309)
                     /locus_tag="VYO12_000473"
     CDS             complement(19989..20309)
                     /locus_tag="VYO12_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1587127.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20471..20551)
                     /locus_tag="VYO12_000474"
                     /pseudo
     CDS             complement(20471..20551)
                     /locus_tag="VYO12_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20595..22592)
                     /gene="metG"
                     /locus_tag="VYO12_000475"
     CDS             complement(20595..22592)
                     /gene="metG"
                     /locus_tag="VYO12_000475"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1587128.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22716..23975)
                     /locus_tag="VYO12_000476"
     CDS             complement(22716..23975)
                     /locus_tag="VYO12_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587129.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(23977..24678)
                     /locus_tag="VYO12_000477"
     CDS             complement(23977..24678)
                     /locus_tag="VYO12_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587130.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24662..25861)
                     /locus_tag="VYO12_000478"
     CDS             complement(24662..25861)
                     /locus_tag="VYO12_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1587131.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26147..27493
                     /gene="gor"
                     /locus_tag="VYO12_000479"
     CDS             26147..27493
                     /gene="gor"
                     /locus_tag="VYO12_000479"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1587132.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            27690..28226
                     /locus_tag="VYO12_000480"
     CDS             27690..28226
                     /locus_tag="VYO12_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1587133.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(28427..28642)
                     /locus_tag="VYO12_000481"
     CDS             complement(28427..28642)
                     /locus_tag="VYO12_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1587134.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28740..29060
                     /locus_tag="VYO12_000482"
     CDS             28740..29060
                     /locus_tag="VYO12_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587135.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29091..29429)
                     /locus_tag="VYO12_000483"
     CDS             complement(29091..29429)
                     /locus_tag="VYO12_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587136.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29441..30160)
                     /gene="trmD"
                     /locus_tag="VYO12_000484"
     CDS             complement(29441..30160)
                     /gene="trmD"
                     /locus_tag="VYO12_000484"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1587137.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30150..30668)
                     /gene="rimM"
                     /locus_tag="VYO12_000485"
     CDS             complement(30150..30668)
                     /gene="rimM"
                     /locus_tag="VYO12_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1587138.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELTLFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30698..31486)
                     /locus_tag="VYO12_000486"
     CDS             complement(30698..31486)
                     /locus_tag="VYO12_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1587139.1"
                     /translation="MKRFEVSTEIGSLSVTYKKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGRNHHYLQWSESEKIAAIIRELSE"
     gene            complement(32063..32302)
                     /locus_tag="VYO12_000487"
     CDS             complement(32063..32302)
                     /locus_tag="VYO12_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1587140.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32322..32594)
                     /gene="rpsP"
                     /locus_tag="VYO12_000488"
     CDS             complement(32322..32594)
                     /gene="rpsP"
                     /locus_tag="VYO12_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1587141.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
BASE COUNT         9672 a         7171 c         5877 g        10032 t
ORIGIN      
        1 ttattggacg gttaccaagt tactcaaagt acagatttga agctgcagat aagactgacg
       61 aaatcacttt aatactaaat gaaaatcaaa aagcaaacta agaagctagc ctcaggctgt
      121 acttgagtac ggtaaggcga cgctgacgtg atttgaagag attttcgaag agtataaaac
      181 actgttttga ggtggtggat aaatcggctg ggtaaaaact caatctcagg agaaaatgaa
      241 gtaaatctcc ccgcaactat agaaaaagat ttgattctgc tcctcacaca ataaaatctt
      301 tttcttttat taaaaacgaa tcgtttcacg tgttttctca tatgaggtaa caaagcgatt
      361 agttacacca ggctctgcca cttccaatgc ttgagaaatc ttgtcaaatg aagcgtgata
      421 atcaaattct ttttcaaaaa tatctaaagc ttcgttaaat gcttcttgaa tgcgttcatc
      481 aaatgagcga tagcggttag aatattgcaa gagttgctct gtcaaagttg catattgtac
      541 aatattataa gtttccgttt ctaaagcttc catatcattc gttgcaattt caagaactcg
      601 ggtaacagat tcaatgttaa tcattttttg ttctaactca accattaaat cctcggtatt
      661 attgcttgcc gtaaagaata acttcaagaa agtttgtgga atacctggca gattgcgttt
      721 ttccatgtat cgcttgatag tatggagacg attgacataa acattggcct tttgacgtgc
      781 attaatatca tctttctcaa tttgtgtcag gcgctcacta actgaaattt gctcatcttc
      841 aatatctttt agttgagttt gtaaatcctc aagattttct tcaagaactg aataagcttg
      901 ggttggttct tcttgattcg aagttacctc aacaatagct gcctcaaaac tctctaattc
      961 tgtctgaata cgacgaacat ggcttgcagc tgtctcagga agtaaatagg tcttgttcaa
     1021 acgtgcaata tcttctccca ataaagtatt attctctttc atatgttgaa ggtaagttgg
     1081 aagagttgca agtagatttt ccactacttt ctgagcagca atttctcgag taaaaatatc
     1141 atacaaggca ttgatttcct cttgtgcctg tccattctca tattcggcat tatccaattc
     1201 caactgacga atattctctt ggtttttctt gaatgcttca taaagcaagt ggaaacgcgc
     1261 ttcaatatcc gtttcaacaa aatgataatt agcatcaatt agtttacgat aaccggcttc
     1321 caaatcctgt aattgatctg gcaattctgt agaaagcgtc gtaaccaagg ctggaacacg
     1381 atccacaata tgacttaagg ccaaaatgtg attttctgta ttatccaaaa tcactgcggc
     1441 ttccacaggg tcacccgatg aattcaaggt tacaaattgt gaaaattcag attggatatt
     1501 ttctaattgt ttttcaattt catccaaggc ttgaccatac tgttctgaat tttcagcaac
     1561 tctatgctga agttcctcaa ataaatccaa agcatgaaga acacgaccac tatttttaga
     1621 ttcttgcttc tctaagtctg ccaaagcatt gcgaattgcc gcaatatctt cttcaatcaa
     1681 agtaatttga ctctcaattt ggtcaatttg atgactggcc ttgagaaaac gaaatgaatg
     1741 gttatagctc tctgcttcaa agagattatt ttcaatatcg gcaaaagagt tgagagataa
     1801 atcgacccat ttttgattcc attcacggaa agccacttga ctttgtccaa tcaagtgcat
     1861 attttttaca gcttctactt catcatttac tggaagattg tatagttctt cttttctttc
     1921 ttctagtgcc tctaatctcc cctcgtttcg cttacgtaga aagattgcca ctacataagc
     1981 cagaactaaa atgactgcaa ttgcaaccat taaataaatt agttgtccat tagacatatc
     2041 aaactccttt tttacttgaa acaatcgtaa cgattatatc atatttttta gaccaaggga
     2101 actacttctc cctatttttt agacatcaag tgtactatag acagcatttt cttcgataaa
     2161 ctcacgacga ggctctactc gatcccccat caacatatca aagattttat ctgcttctgc
     2221 agcatcatct acagaaactc tagccatcaa gcgatgttcg ggatccatgg ttgtttccca
     2281 cagctgatga tcgtccattt cacctagccc cttataacgc tgaatagtcg gtttggtacg
     2341 accttcacta taacgggcta aagcttcttg gagtttgatt tcttgatctg cacccggctg
     2401 gatatattct ttaatctcgc ttccaacctt gacaccatag attggtggtt gggcaatata
     2461 aacataacca gcttctagga ttggtttcat ataacgataa atcaaggtta aaagaagggt
     2521 acgaatgtgg gctccatcga catcggcatc ggtcatcaaa acgagttttt ggtaacgggc
     2581 tttcgaaaca tcaaattctg cgccaaatcc tgttcccatg gctgtgaaaa gactacgaat
     2641 ttcttcgtta gctagaatct tatccatact tgctttttca acgttcaaaa tcttaccgcg
     2701 aattggaagg atagcctgaa actcacggtt acgaccagat ttggctgatc caccagctga
     2761 gtctccttcg acgatgaaga gttctgtttc agcagggtta ttagaagaac agtctgctag
     2821 tttccctgga aggttggaaa tttccaaacc agatttttta cgtgtgactt cacgcgcacg
     2881 cttggcagcc acacgagcct tggcagccaa aattcctttt tctacgatac gtttggcaat
     2941 ctgtggattt tccatgagga aatcggagaa ggcttcactg aagaggcgat tggtaatctt
     3001 gaccacttcg ctatttccca atttggtctt ggtttgtcct tcaaactgtg gatttgggtg
     3061 tttaactgag ataactgcag ttaagccttc gcgaacatct tcccctgtta ggttgtcttc
     3121 attgtctttc agtaacttat ttttacgagc ataatcattg ataacacgtg tcaaggctgt
     3181 acggaaacct tgttcatgcg ttccaccttc atgtgtatga atattattgg cgaaactcat
     3241 gacattttca tggtaaccag ttgtgtactg catggctacc tcaactgtga tatcatccat
     3301 ctcaccgtct gtatagattg gtgtatcaaa gattacatcc ttgttctcgt tgatatattc
     3361 aacgtaacta gcaatcccac cttcataatg ataatgcttg gtttgttcca aaccttggcg
     3421 cttatcagtg atagaaattt gaagaccgcg atttagaaag gccaactctt gaatccgttt
     3481 atttaattta tcaaaatcaa agattgttgt ttcagtgaaa atttctgggt ccggtgtgaa
     3541 gtgaaccgtt gttccagttc tatctgtatc cccaaccacc tcaaggtctg caacaacatg
     3601 accacgacgg tattcttggt aatgaatctt accgttttta tggacatgaa cgtctaattg
     3661 agtggaaagg gcattaacta ctgacgaccc caccccgtga agaccacctg aaaccttgta
     3721 tccaccaccg ccgaactttc ctccagcatg aaggactgta aagacggtct caacagcagg
     3781 acggcctgtt ttttcctgaa tatcgactgg gataccacgc ccatcatcca caacagtaat
     3841 cgaatcatct ggctcaataa aaacttgaat atggctggca aatcctgcca aggcctcgtc
     3901 aattgagtta tcaacaattt cccagactag atggtgaaga ccttcttttg aggttgatcc
     3961 aatgtacatc cctggacgca tacgaacagc ctctaagccc tctaaaactt gaatttgact
     4021 ggcatcataa tcctgtgcct gcagattttt gatttcttct gtcatcttat tcctttttct
     4081 tatatgtcta atacttgtct taaattcacg atactggtaa acaagtgggt atcaagtcct
     4141 gcagcttgac ctgcttcgat atcaatcggc cgatcaccaa tgacaagacc agagctaatc
     4201 tgatactttt ctcttaaata aagcatggat tcgggatttg gctttctctt aaagcctgag
     4261 ctagaagtca ccacttctgt aaaataagct gctatagagg ttttttctaa aatttccaaa
     4321 acctgatcat ttcgatgaga gaccaaaaaa tgacggccac cttgatttga aatgacttcc
     4381 aataggtcag aaactccttc aaataaaatc gggtgttcaa gctctctggc ttcattttcc
     4441 ttgtactttt ctaaaaaatt ctctaaattg ggagcgaatg tctcaatcgc aaaagaagta
     4501 gaaaccttta aagcttgata gacactgtca tggtcttgtg tgataccata cagtgccaat
     4561 gtttcaacaa atgcagctgt tgaagtttca taattatcca gtaaagttcc acctaaatcc
     4621 cagatgtaat cgtgatattt catacccttc attatagcat ttttttatga aaaaagcgat
     4681 gatttccctg agttttccac ataaaaaacg agagaataac tcccgtttta ctgattttta
     4741 ccaaagacat ctcgatagag cattcctgtt cgtaaactct ctgcgtcccc tcctagttgc
     4801 tccaccaact cgtaggcaaa ggcaagggct gttgaaggac cccgactggt tgtcaactga
     4861 ccatctacca ctactgtttc cttgacgtag tgaccatcaa ggatttgctc ttgaacgccg
     4921 tcataacaag tgtatcgctt atttttcaat atctctgctt gattgagggc aattggtgcc
     4981 gcacaaatgg ctgctagttt cttcccttct tgctcgaagc tttgcaattc ttgaatcaag
     5041 gtctgattat cacgtaaatg tgcagaacca ggcatacctc caggaagaac aatcatatca
     5101 tagtctgata aatttccatc aaagacatga tctgctctta cttggattgc atgcgaaccc
     5161 gttacttgct cttcaaaacc aaccatatca catgtgatat tggctcgacg caagacatct
     5221 acaactgtca aggcttcaat ttcttcaaag ccctgagcta acataactgc tacttttacc
     5281 atgattttct ccttatttaa tttgttttaa tacaaccttt ttccgtttga atgggacttt
     5341 tccgctcttt tcttcagcta gattagtctg gtaactcatc gataaaagca aaccaacacc
     5401 aatcagatta ctgataatag ctgatccccc ttgcgaaatg aaaggcaagg gaatccccgt
     5461 caaaggaagt agtccagtca cagcaccgat attctcaaag atgtggaaga gcaacatcat
     5521 aatcaaacct gtggaaatat aagtgtagaa ctggttattt gatttaagag taatcttcaa
     5581 catacggtaa atcaacatga gatagagggc aataaccagg acagagccaa taaagccaaa
     5641 atcttctgca ataaccgtaa aaatcatatc tgactctcga actgggataa gcagattcga
     5701 agcattaaat ccctgaccaa ataagccacc actcccaatg gcaatctgcc cttgagcctg
     5761 ctggtaagtc gttgtttggg caaactcaaa gggattgagc caagccaaaa tccgattgat
     5821 ttggtaggtc ggcattccaa tctggtgaag aaaagctcgt ccgtccttgc taataaagat
     5881 agctaagaaa ccagcaactc ctgttacagc agtcacaaat actgggataa taattttcca
     5941 agaaacccct gataataaaa cgattcctga gaaaatggct acaaaaacca aagccgtccc
     6001 caagtcactt tgaagtgcta aaagaactag gactggaatg gtaaagagaa tcatccagaa
     6061 aattaacaaa aagtccagcg gaaccgtgcg tctccattcc ttatgtttct ttgtaaattg
     6121 gacaatgaca cgagccaaca tgaggatata agatatcttc ataaattctg acggttggaa
     6181 tagggtaatt ccatttattg atacccagtt tttggcaccc gttgatgcaa ctaatcttgg
     6241 attataaaat acaatcggca agatcataag tcccaagcct aaaatatata gaaagggggt
     6301 caccttccaa agaaattctg tattaaagag catgaccaca aaaccaatca caagccccaa
     6361 ggcaatccag gcgacctgct gccctaaaat gggcaaaata ttattgggat aatcatgact
     6421 aacggctata tagatagcca ccacaccgat gaccagtaga aaaaatactg gcaagagcaa
     6481 actgtaatcg actcttgagt cgagagaacg tttcatatat actaacctta tactttcata
     6541 caatactatt tatcaaagtt cattaaaaaa tctatcaata gcctcgtcaa cttcggatcg
     6601 agagatggtt ttaacagtcg cttcttctgc tagagatact actatttgtt tgccgtatcg
     6661 ttttccgacg attctcctat ccaaaataag agttaaggaa cgttggtatt cacgtctcat
     6721 acttcttccc aaagcctgtt ttaaacgaat aatggccatt ggcaattgat aatcataaaa
     6781 ggcatttttc ccttcttgat tcagttcttg attaatcttt ttcgtcaagg gttcttgagg
     6841 attttggaaa ggaagcctcg gtacaacttg aatcacaaaa ggatggcttg aaaaatcaac
     6901 tccctcccag aaacttgctg caccaagcaa gatttgttgt tcaccttttt caaagcgttt
     6961 ctttagctga tgaacatccc catttttata ctgggccaag tggctaactg taagtaaatc
     7021 cgatactgct agaagcatgt ctttagcggt aaagagaacc aaaatcggtt gctggaaagc
     7081 ttgaatttcc actagtaaag cagctacctc tctggcatag acttctaagg aggtttctgt
     7141 taccagaggg aaatctttga ccatgaccac ttcttgttcc tgttttcccc gagattcaat
     7201 cttgacaaat ttggcttcag gatagcctaa aaggtctgcc aaagaaaccc tctgactaat
     7261 ctcaagagta gccgatactc ccaagacttg gcaactctca ggaagtaaag aggacagaag
     7321 agtacggcat gattttgtag aagaaatctg aattttcttt tgactcgttt cagttacttc
     7381 aagccagtaa tcaccttcag ctgtaaaata gcgaaccagc tcatcaaagc cttctacttc
     7441 taattctgaa aaatactgat ggagattgtc cagagaatct aagatatttt tcctagattt
     7501 accagactga aattgttcta tcaagtagag acactcaaag cgaatacttt ctagtatccg
     7561 ttgttgaacc ctgttttctt ctcctactaa agccttatca attaaatcga taatagattg
     7621 tatatcgtag gtctcttgaa gcagattttc tagagctaac aaaatctttt ggacttcatc
     7681 aataatcagt aaacggtcac tgacaaattc aggattatct tcaagtctgg tcacgagata
     7741 ggcatgatta gtcactaaaa gcttgcaagt ctctgccctt tcttgactac gtttccaaaa
     7801 atcttccgtc acaaataagc tctgggatga taaattccca tcatgacgaa ggtctgctag
     7861 aaaatgttgg taacggtaga gttgcccgat ttcatccaaa tctcctgtct ctgtctcagt
     7921 aagccagacc aagacttgca ttttaaagcg tctaaataag cgattttcat cattttcctg
     7981 caaggaatga taaaaggcat ccaacttcag ataattttgt ggtcccttta agctatgaat
     8041 atctgtatgg aacacttcct tgaggcgttt accttcttct tccatgattt gattttgaag
     8101 aatctttgtc ggaacactaa gaacaatttg tcgctctttg gattgagata aagcgggtaa
     8161 gagatagcca taggttttcc caatccctgt cggcgcttga atcagagaga caggttcatc
     8221 tttcaatagc aagccaacct ctttagcaaa actttcttgt tcctccctca cttcaaggtt
     8281 caacagagaa atatttttag aaaagtcttg agatagtttt cgtggctcca gagaagctgc
     8341 cgttttctta aaatacagac cttgaacttg gaccaagtct ggagaactca ggatagattg
     8401 gttgcgataa gtttcctcaa taaccaggta ggactcatat aggagagcgt cagccatttc
     8461 cagcaagcgt tccaagagac ctttaggaag ctgggtcatc ttttcccgta aaaaaagaag
     8521 taattctgct gtagcttggg catctgaaag ggctgtgtgt gcgtgtttaa gaggaattcc
     8581 taattctcga cacaaaatcg gcaagctata tttttccagt tcagggaaaa agacctgggc
     8641 caattcgacc gtatcaacac gagggtttct tagctcatag ccttcaaaaa ataaattttc
     8701 cgccaagaga ttagcatcaa actgaacatt atgggctaca aaaatcccat cctccaccaa
     8761 gtcaaatatt tttctggcaa cttgcgaaaa atcaggtgct tgcgccagac gttggtctgt
     8821 caatcctgtc agttctttga tatgagcatc caagggttca tgtggattga catccgtcgt
     8881 atagtgatcg acgatttctc cgtcctcaat cacgacaatt cccacttgga taattttagc
     8941 cttactacct gtgctagttg cctctaaatc caccacaaca tagcgattac caatcgtttt
     9001 cattataaaa aattatacca aaaaaagggc catttggcac ctgcaaacat aggataattt
     9061 tcaaactcaa gaacactact tttcttaaaa atcccatcga atagagcatt ctagcctgct
     9121 gatttttgta aaatagtgaa gaaatacgaa aaattgagag aagaaatgtt tgcatcttcc
     9181 ttctctcaac gaatcatgta ttttatctaa ccatgtcagg atcgtagtca taaaatcctg
     9241 tatcaaggaa acggttttta gggtgaagtt tacgaacttc ttcatccagc aagaaggctt
     9301 gatcatggct aaagttgccc tcttgaatca agttacgcgc ttggataaag agttttgcaa
     9361 tctcaacaaa gttctgacca ggagtgtaga gcccttctag tttccagcga gtaaagccat
     9421 gctccaccaa ttctgttaat ttgatcatca aatcaaggtc attgttggca aagatatggg
     9481 tcccatgatt atcttcaaaa atggaatagt ggctctctgg atcacttggc tcagccaaga
     9541 agaggtcacg tttatgcgtc ttttcatcat cgatatgtgt aaagttatag tagttttgca
     9601 agagtggacg tttagaatga tggatgacgc tagcaccgta aaccaaaact tcagcaggaa
     9661 tttccaaaat ctctggcatt ttgaaaagtt cagctgatgg aatttcacgc gccaaaacag
     9721 cctcagatgc gccagccttt tgtccccaga agttaatctg acgactgcta gttaccatgg
     9781 ttgaagcatc gtagatggtc ttaaatgaat aaccatcgcg gttaactacg taaaagacgc
     9841 ctgcatcccc aatcgtaata tagtctgtct tgatttcttc caagaagtct aagaaaggct
     9901 tgatacggtc catcatatct tggtgcatga gagcattgac cgcaacgatc aattccttac
     9961 cagcatcatg aaccaactta gcgatttcac gtaattggtc ataactaaag gtcgttggca
    10021 gacgaaggcc aaaatctttc tcaccgacat agatacggtc tacgccagct tcgagtagtt
    10081 gttcaacttg ttcaatactt tcagcagttg ctgtaatgac aatcttttcc ataagaaaaa
    10141 ttataccaca tttctccaaa atcatccatg ctttgaaaat gaaaagggac tttattcttt
    10201 ttgtaacctt tctgtaataa ttctctgccg aaaaaatgat aaaataggta tgtactgtta
    10261 aggagagaat tatgcccgta agaaaattac aatcctatga ggtagactat caagaagaat
    10321 taaaccagca gcttcctcat taccaagctt atacacctga agcgcaatct gatgccaatc
    10381 tcaaggaaat cctatttttt attaatatcg ctgttttttg tatctgtatt gctatcttta
    10441 gttttatctt tttagcatta aaattatcaa ctgctcttgc ctttgccgca gcaatcggat
    10501 tcagcttact tgttttaaaa gttcaacggt ctattatcaa acgaaaacgt agaagataaa
    10561 tccaaaatat cgccccagtt ggagcgatat ttttattttt tctttttaga tggtgtctga
    10621 atggcaatct tcacttcaaa gattgttccc ttgggtttat tatctttgac agtaacagtt
    10681 ccttttagag catctacaat ttgcttggct agggataatc ctaaaccaaa accacctttt
    10741 tgccgggttc tagccttgtc tactcgataa aaacggtcaa aaattttctt tttatcttct
    10801 gtcgaaatac cgattccatt atcagaaaca agtaaataaa gattgcgatc ggtcgccgag
    10861 ataagaaaat caatttcacc atcctcctca gtatacttga cggcattatc gaaaagaatg
    10921 gtcatcagtt gtttcagaag aagctgatct gtgacaattg ttcgatggat acgattttca
    10981 aaacggaaga cacgattatt ttccgaagca atcatctcgt agtttgtgaa agttgtatta
    11041 aaaaagctag ttggaacttc tgcaagctcc ggcttaatcc catcatctct ccgagctaag
    11101 ttcagcaagc ttgtcgttaa aaaacgcata tttcggactt cttccaaact cgatgcaatg
    11161 ctttcgctca catccataat ggtagcttct ggcttacgaa aaagggtctc taagcgattt
    11221 tgcaaaactg cgagtggagt tcgtaactca tgactggcat tttccacaaa agactgttgc
    11281 ttctgcatac tctcaagcag gggcctaaca ctgaccctag ctagatagag actggcaagt
    11341 aaagacaaaa tccagaaact agccatcacg accacaatca attgctcatg cttttgacta
    11401 gcctgttcca actgactggt attaatcaag acagcagcat acttgatatt ggttgaaacc
    11461 gaactaatat tggtctccat caaaatcaca cgatagattt cttcctgccc atagctatta
    11521 aaaacctgaa tctggtagat atgtcctagt tctttctttt ctaacttaat cttatccaag
    11581 cccaaaaatc gatttccaga aagaagttga gtaaagtctt tatcaaagag aatgacttcc
    11641 gtattggaac tgacattagg ttttatttct actttactag cgtccgccct agcattttct
    11701 aaatctttaa tctcttctgt tgccctattt atagccagct gaataactgc ttgaggattt
    11761 tcactcagtc catgaagctt atcgtccacc gaagtataaa gactcgaatg catgacttgt
    11821 aaaataatca gagtcattgt agaaaaaatc agggtgaaga caccgaagtt gcggataaaa
    11881 taactaaagt catccgcata ccatgttttt ttaagtttac tgaacatctt ttaaaagata
    11941 cccaacacta cgcaaagttt gcaaattctc tgcaaaagtg gttcccttta atttcttacg
    12001 gacttttgaa acatagactt cgacaaccga aatcgttgta tcactatcaa atccccatag
    12061 acggtcaaaa atctgcgtct taggtaaaat cacattttga ttttgaagga aataaactag
    12121 taaatcgaac tctttcccca gcaattcgac aggagtatct tcaactttaa cggtattggt
    12181 tgataaatta accacgatat tcccataagt caaggtgttt tcattaaact tccctgaacg
    12241 tttgagaaga gcctgaatcc gcattttaag ttcttctagg tagaaaggtt tggtcagata
    12301 atcatccgct cccagttcaa atccatgtcc cttgtcatcc aaactttcct tggcagtcat
    12361 aatcagaact ggtgtcgtaa ttcccttttc acgcaattct tttaagactt ggaaaccatt
    12421 tttttctggc aacatcaaat ccagcaaaat caagtcatag acaccactct cagcttcgta
    12481 gagaccttct tctccatcaa atacctgcat aacatccgca aaatcgtcta aaaagtcaaa
    12541 tactgaattt gacagaccta ggtcatcctc aaccaataag atttttatca tgagaaactc
    12601 ctccttatta aaactattat accaaatttg ccttaaaaaa aactcaactc tctgcatttt
    12661 acatgagata gctgagtttt ctttttattt taggcttatt tatgcatttc cgtattgaag
    12721 aacaactgct tcgactgcag ctttttcacg gctaatcaag tcaacacgcg ctgcaatttc
    12781 cttgattccc ataccgatgt tacggctaag agcaaggtca gaaagttgcg gctcaaagaa
    12841 ctccttgtat tccgccaagc gttgctgagt cttaaataca tgagcaggaa ggataacaaa
    12901 gctatcaaag ctcatatctc ctccaagggc tgccttaatc caagcccagt tttcacgcgc
    12961 ccaagaccaa gctgttttct gagttgcttg atgagctagg aattggtaat accaagcaga
    13021 caagtcctgt ggtttgacca caaatttgtc cttccaagaa gtaatcaggt tttggatatt
    13081 atccgcatct gtactgtatg caagagctgc tgccaactgg cgtttaaaga cagcatctgt
    13141 tgcgtgagta taagtatcaa gataaagtgc taacaagtct ttagtctcat gatgtttcat
    13201 ctcattaatc agaacttgtg agcgaatagc tgctgggagt cctgcaagat tctccttgtg
    13261 tgttgcgaag atttggctag cgacttgact agcttctgca tcatttgagc gaatcatcat
    13321 cgaaacagcc agctgacgaa ccaattcatc ctcatctgat tctccgtctt tagcttcaaa
    13381 accaagacgg tcatagttat gacgagccaa tttagcaacc agtcctttga aggctgtttc
    13441 agcatccgtt ccttcatcaa taaagcgctc aagggctgaa atcacttgag aaacagctga
    13501 aaccaccaga taagactctt ccttagcaag tttatcaaga actggaagca agtctgcata
    13561 agaaatgtgc cctgcctcag ccaacaaacg acgttcttga acaatttgca gtttgcttgt
    13621 gttatcaagt gtctctagct cagcaagaac agcttctaac aagtctcctt gatagtcggt
    13681 aatatagtgg gcagtatttt cagtgttgag acgaagagct ccttcatttt cagcaagaag
    13741 agctgcgtag ccagggattt cgatactttc agtttcgagt gtatcaggca agcctttcca
    13801 gttgctattg aggggcacca cccagagacg gttcttgtct tcgttctcac cgatgaagaa
    13861 ttgtttttgt gaaatcttca agacatcatt ttcaacttta acagtaagaa ctgggtaacc
    13921 aggctgttcc aaccaagaat ccatgaaggc tgcgacatca cgtcctgacg cttgaccaag
    13981 ggcatcccaa aggtcactac caatggtgtt gctgtattgg tgtttttcaa agtaggcgtg
    14041 caaaccttta gcaaaatcag catctcctag ccaacggcga agcatgtgca tgagacggct
    14101 tcctttggca tagacgatag cgccgtcaaa gagtgtattg atttcatctg gatgtttaac
    14161 ttcgacgtgg acagactgaa cgccatcagt agcgtcacgt tcaagagcaa gaggtactcc
    14221 acctgtttgg aaatcttcaa agatattcca gcttggttcg atggtatcca cacagacgta
    14281 ttccatcata ttagcgaaac tttcattgag ccaaaggtca tcccaccatt tcatagtcac
    14341 gaggttccca aaccattggt gagccaattc atgggccaca acaagggcaa cttgttgacg
    14401 gctagcaaat gtagagttct catcgacaac caagtaaact tcacggtagg tcacaagacc
    14461 ccagttttcc atagcaccag ctgagaagtc aggaagggcg atgtggagag attgaggaat
    14521 tgggtactta actccatagt aatcttcgta aaactcgata gagcgaacag cgatatccag
    14581 tgagaaatca agatttgaaa gtggatgtgc tttggttgag tagacaccta ccagggtacc
    14641 atttttagtt ttagcggtca ccccttgcaa atcaccagca acaaaggcca acaagtaaga
    14701 agacatgcga ggtgttgtct caaacttcca gatacctgtt tccttacggt tttcaacatc
    14761 gatttctggc atgtttgaca aggccaattc accttctgct tggtcaaagc gaagagagag
    14821 gtcaaaagtt gctttggctt caggctcatc cacacatggg aaagcttcgc gcgcaaaatg
    14881 gctctcgaac tgagtagaca agacctcctt cttgactcca tcaactgtat aataagaagg
    14941 gtaaatccct gtcatgttgt ctgtaatttt accagaaaag gcaagaacca attcaacttg
    15001 accagcctca gccaattcga tatgaagggc ttcattgtca tggtcaactg taaatggacg
    15061 agcttgacct gcaacttcta cagaggtgat ttccaagtct ttttggtgga gggagatgcg
    15121 gtcactctgt gcttgaccag tgatggtcac tttcccagaa aaagtcttgg tctcacgact
    15181 caaatctaaa aataaatcat aatgttcagg aacaaattgc ttaataaaat gttcaactgc
    15241 ttgcatagtt ttctcctatt ctaagtttaa gagctgctgc tcttcttcaa acaaacttat
    15301 tatatcatat tttgcttgag aaaaaaggat tggacagcga tttccaaaac tttcttcctt
    15361 ctaatagtct acagtgggta gaccttgcga aattcttcta aaacaacctt gctttcaggt
    15421 gtaaaagtca gccctacttc acccaaacag tctgtgaaaa agtcctcatg aacctggcgc
    15481 caataggcaa gtgatttgtc tccctcacct tccttaaagg catgttgcgc agaaacttga
    15541 ttgaagagtt caacagaaac ctttgtaatt tcgacaatgc agacagactg attttgacta
    15601 tctaaaatga catcgaaggt ccctacttgg ggaagtggtt cgtcttctag tacatagagg
    15661 tcataggctg atgctgttgc tgtcttttct cctttaaaca ccaaatccgc taaaaggtct
    15721 ggttcaactc caaaagccca agcatcgatt tcatctccga tcaaaggatt gatttgcttg
    15781 tatttattcc acatttcttg cggtatcatg ggtactcctt tgtaattttt tactttcttc
    15841 ttttatgtgt ttaagatgat ctggatggtc aatctctaaa tcaaaaatct ctggaataga
    15901 actgtagtgg ataatgcact tgatacccaa ctgattcatt ttttgtatga aagaagtatt
    15961 cagatagcct gctacagcaa aatcaatctt gttctttctt gctttatcct gcatatctct
    16021 tagcatatct aacattattg gactttccat atcatgccat tgactgtttc tcatagtcgc
    16081 aaaaacaaag gaagtcaaat cattcattcc aactacaatc tttgaaatgc ccgtttccag
    16141 tatcctatcc aagtcaaaat acgctgacgg taattcaatc atcgttccga ctttcccagt
    16201 aaagccatac tgacgcaata ctgtaatagc ttgttttaat tggtcggcat cattgacaaa
    16261 aggaaagata agagatagat tgggattggt ttgataaact tctgtaacga catgtgcttc
    16321 agcctgaaat tcatccaaac acgccagtaa acgcctggtt cctctatagc caaacaaggg
    16381 atgcccttcg tcaaaaaact ctctagtccc cactaaacaa ttggcttctg tattcgttaa
    16441 ttcagaaaaa cgataccaaa cttcttcatc tgagtacagg gagcagatag tctctagata
    16501 atcttttaca aattgctgac aacttgctaa tatgatgttt tgattaagct ctctcaacaa
    16561 atattcccca cgaatcatgc cgacgtggtg aaatagttga ggataaattt tttcaagaat
    16621 tttttcgcca ctaagggcaa gttgatttct catcattcac cttccaattc atgtaagaag
    16681 tcttgtccag ttctggaaat cctaataatt cagacttaac cttcaagact aatggcgatg
    16741 cattttcttc tgtaatctct tgaatatcca tccaaatata tccaagtgaa tcattcgcac
    16801 catcagacac agcttccgaa gtcgtaactt gaggtgcact ctcattcatt tcaacatcat
    16861 acaaggccat gacatggtga accataaaat ttgttaactc ttctctgacg aaaacatcgt
    16921 agattcgagg attagagtag cttctaacag taaatcccgt ctcttccata acttctctag
    16981 tcagcgtttc cgtcagtcct tcaccaagtt gctgactgcc tccaggtaga tcataccgat
    17041 gttgataagg gcctctcgtt ttttcaatgc agagtaactt cccattttct aagcaaacag
    17101 cgtagactcc aaagtgtttt ttgatttcca tccaactcct cctacttcaa agaccagcca
    17161 ccatctattg tcaagatttg tccttgcatg gcgctcgctt ttccacttgc taaaaaaaga
    17221 ctaagctctg ctatttcctc tggctcaatc cagcgcttga ttggggtttc actagccacc
    17281 cagtcagcca aaccacctgg ttcaaaatcc gcagcggtca tagctgtctt gactgctcct
    17341 ggagcgatac caaagacctg aatcccagct tcagcatagt ctagagccaa ctgcttggtg
    17401 aagccagcca aggcatgctt ggatgaagta taggcgtgac cacctccacc tgctaggcta
    17461 gaagcaatgg aacacatatt gatgatgatt ccctttttat tttccagcat ttgtgtcaaa
    17521 taataccgag tcaactcaac aggagtcatg tagttaattt caaaaatctc ttgaatttcc
    17581 tgcgcagtct gttccaacag tggtttgtaa gcatccaaaa ctccggcagt attacacaaa
    17641 acatccacct gagggcacca gtcaaaaata ggttccaagt ccaaggtcaa atctctctgt
    17701 aaaaagtgaa aatcaccctc taagagtggc ttttcacctt ggtcaactcc ataaacttga
    17761 tagcccttct ctaaaaagag gcgagcttga gccaatccga tccctgaact cactcctgta
    17821 atgagtacac gtttagtcat gcacttctac ccaatccgtt gccaaaacat cacaagctgt
    17881 cgggctccac atggaaaaac cttctccttc tccagacacg ttgattaaga aataaggtgt
    17941 cacttcaagt gcaagcccat tttgctcgat ggtatcaaag agttggacat agttttcagc
    18001 acctccccaa ccagttcgta catatttttt cttagccttt aagccaggta gaatttcttc
    18061 aaatgtcatg tttttctcct ttaatgtcaa taattctccc cttcattata acaaaaaaac
    18121 cgctttaaaa cggctttttg actgtgattt atttacatct tcttctactt acggcaaatt
    18181 attccctgca gcaagataaa tttcatacca ttcttttctt gttaagctaa agtttgccgc
    18241 ttggctaact tctctcaagt gcttaggatt tgtggtacct acaactgcct gcatttttgc
    18301 tggataacgc aatatccaag aaatggcaat agttgaagag gttactccat atttaatagc
    18361 taaacgatca agtacttgat ttaaagcttg aaatttctca tttccaacaa agttcccttt
    18421 aaaatacccg aattgcaaga cagaccatgc ttgaatgacc acatcgtgta atttgcaata
    18481 ttcaaaaatg ctgccatctc gcatagctgc ttgactatct tccatattaa catgaaaagc
    18541 tgattcaaat cctggagtaa aagccgcact caattgtagc tgattaacag ctaacggctg
    18601 cttgacatct tttttaagca actccatcat cataggattt tgattagaaa ctccaaaatc
    18661 tcgaacttta ccttgtttat aaaggagatc aaaggcttct gctacttggt cagattccat
    18721 caaagcatct ggtcgatgaa ggagcaagct atctagatga tcaatcttca atctttgcaa
    18781 aataccgtct actgatttta taatatagtc cttagaaaaa tcaaaatagg taaattcttc
    18841 aatgcgaatg ccacatttgg actgaatcca catcttttct cttaaatctg gacgattttt
    18901 taggacaaga cctaacagtt cttcacaacg accacgacca taaatatcag ccaagtcgaa
    18961 ggcattgatt ccaacagaaa gtgctgtttc tacaagctct tcaacttctt ttacagactt
    19021 atcttttatt ctcatcattc cgagaacaat ttctgataat tctttgtcat cttgaccaag
    19081 agttatgtat ctcatcaaat ttttctcctt taatttctaa cattcttccc ttcattataa
    19141 caaaaaaccg ctttgcaacg gctttttgac tatacttcac tccattttat cttcttaaac
    19201 ccacggaaca agacaaagat tccaataaag aggacagcta aaggaataac ttttgtaagg
    19261 aaaacatttg aaattcccat ccactcataa taacggagca gagaacccac cacaagatgg
    19321 gcaataatca tactgacaaa tggacgaaag accgcttctt tccaattcca aataccgata
    19381 actagcgaaa tcgtaaagac agacaaacta tcccagggag ccggaatata aaaggctcct
    19441 tcttgtatga agcttgccat tcctacatat cctaaaacaa ctagaagaac tatagtccca
    19501 acaacaatgt aagtgccaat tttcatttta ggagaatctt ggactaaact tcttcgtaaa
    19561 attgtggcca caagtccaaa tccaatcaga aaaataagaa gttgccctaa aaatgtgagc
    19621 aaattgactg ttaagagagg acctttagaa aaatcactta gtagttgata ataacgtaat
    19681 accgccagga caagaattgg cgtcaaaagg gactctttga tagaactgcg aggtgctccc
    19741 ttgagaatct ctttcattat ttttttagga ttcttaccta gataatcctc tgcactcatg
    19801 ccatctcgtt ctgcttctga gaaatctagc atcatcaaat agatctgctc tctgagatag
    19861 tcttcatcat agagaaatcc agcaagatta aaactttccc acaactcctc aaaatacttt
    19921 tgattctcct cagaaaactc atgtagcaaa gcgcttgttt cttcgtaata cttcattttc
    19981 ttcatggttt aacccccatt cttaatccct tctacttttt gactcaaatc gtcccattgt
    20041 tgccaaaaga ctgagacacg ctcttctcct tctttcatta atgaaaaata cttccgatct
    20101 ggaccatctg gcgacgggcg catgtcgcct cttatccatt gatttttttc taacttttgc
    20161 aacaaaggat aaatagttcc tggaacgata gtatcaaatc cagcctctcg caaagtctga
    20221 accaactcat aaccataccg ctctttttga ccaatcatat ccaagacaca accttcaaga
    20281 acacctttta atagctgagt ttctttcatc acttctccct tctaatctat tttgtaatac
    20341 ctactagtaa cttcacctat agtatatcac ttctacacta gtttgtaaag cataatagtt
    20401 aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cctaccgtat gtatggttac
    20461 tgacttcgtc agtttcatct acaacctcaa aacagtgttt tgagcaacct gcggctagct
    20521 tcctagtttg ctctttgatt ttcattgagt ataaataaaa aaacagaact agtctgaact
    20581 agtcctgtct acttttaccc aatcacactt ccatttggta cagctggatc aactgtgaga
    20641 agggttaatt tgccatcatg ttcagctgat agaatcatac cctggctgac atattttttc
    20701 atcattttac gtggtttgag gttagcaacg atttgaactt tcttgccgac caattcttgt
    20761 tcatttggat agtattttgc aattcctgaa agaatctgac gatcttctcc atcaccagca
    20821 tccaagcgga attgaagcaa cttatctgaa ccttctactt tagacacttc tttgacttct
    20881 gcgacacgga tttcaacctt gtcaaagtct tcaaacttga tttcatcctt gtttagtttg
    20941 agctcaactt cgtccggatt ccattctttt tcgactgctg gtttattgcc ttccatttgt
    21001 tccttgatat aggcgatttc ttcttccata tttagacgtg gaaagatagg tgttcctttg
    21061 gcaactacag tcacatctgc tgggaagtca gccaaactca agttttcaag actagaaact
    21121 tcttccaaac caagttgagt caaaactgca cgactagttt ccatcataaa tggttcaatc
    21181 aagtgagcaa ctacacgaat gctggctgcc aagtggctca tgacacttgc caattggtca
    21241 cgaagagctt catccttggc caagacccat ggtgcggtct catcgatgta tttattggta
    21301 cgagagatca gagtccagac tgcttcaagc gcacgtggat agtcaactgc ttccatgtgt
    21361 gtatggaagt ctgcgattga ttgttctgca acctcagcaa gaacatgatc aaattcagtc
    21421 acaccttcta cataggcagg gatttgtcca tcaaagtact tattaatcat ggaaaccgta
    21481 cggttaagga ggttcccaag gtcattagcc aattcatagt tgatacggcc gacatagtct
    21541 tcaggagtaa aggttccgtc tgaaccaact ggaaggttac gcatgaggta gtaacgaagt
    21601 ggatctagtc cataacgctc taccaacatt tcagggtaaa cgacattccc ttttgactta
    21661 gacatttttc cgtctttcat gacaaaccaa ccatgggcaa tcaaacgatc aggtaattta
    21721 acatccaaca tcataagaag gattggccag tagatagagt ggaagcgaag gatatctttt
    21781 cctaccatat ggaagactgt tccattccag aacttgtcaa agttaccatg ttcgtcttga
    21841 gcgtagccaa gagctgtcgc atagttaaga agggcatcaa tccaaacgta gacaacgtgt
    21901 tttggatttg atgggacagg cactccccat gtaaaggttg tacgagatac cgccaaatct
    21961 tccaagcctg gctcgatgaa gttgcgtagc atttcattaa ggcgaccatc tggcgtgata
    22021 aattcaggat gagctttgaa aaattcgacc aaacggtctt ggtatttgct aaggcgaagg
    22081 aagtatgatt cttcagaaac ccattcaacc tcatgacctg atggagcaat accaccagtc
    22141 acatttccag cttcatcacg gaaaacttct gccagctggc tttctgtaaa gaattcttcg
    22201 tctgatactg aataccaacc agagtattca cccaagtaga tatcatcttg agcaagtaag
    22261 cgttcaaaga cctgtgcgac aactttttca tggtagtcat cggttgtacg gataaattta
    22321 tcgtatgaga tatctagtaa ttgccagagt tctttaactc caaccgccat tccatcaaca
    22381 taggcttgag gtgtaatacc agcttcttcc gctttctgct ggattttctg accatgttca
    22441 tcaagacctg tcagataaaa tacatcgtag cccatcaggc gtttgtaacg tgctaggaca
    22501 tcacatgcga tagttgtgta ggcagaaccg atatgaagtt tcccagatgg atagtaaatc
    22561 ggcgttgtaa tataaaaatt tttttcagac ataatttttc ctttccaggc aaatgaaacc
    22621 tgtttttcta acacttcatt atatcacatt tttaatgaat ttcgataggg aaatccatac
    22681 aaaaacaaga tagacgagtg tccatcttgt tgatctcatt cataacgaag ggcttcaatt
    22741 ggatcaagtt tcgatgcctt gttggctggc aagactccaa aaatcatacc aacactagcc
    22801 gaaactgcaa gactaaatag ggcaactggg attgatactc caacttctat accttctatt
    22861 aaaccttgca gtaacaaacc tgctaaggca gttaaaccac ttgcaattgt caagccaatt
    22921 aagccaccta acaaggtcaa aatcatggat tcaatcaaaa actgaattaa aatattggca
    22981 cgtgttgcac ccaaagcctt acgaagacca atctcacgag tgcgctctgt caccgaaacc
    23041 agcatgatgt tcatgacacc agttcctcca acaaagagag aaatccctgc gatggaacta
    23101 ataatcgtcg tcataaaact aaacgattgt tgaatttctg caaatacaac ggactcatct
    23161 gccacctggt attctccctg ttgtaagcct gcaagctctg tcatttttcg tgccagttct
    23221 ggacccagag ttggggttaa actggtatca ttcactcgaa agacaatatt agctatttca
    23281 tctacattaa aattcgcagc aagggagata ttggtagtaa taggcaagcc accaaaccca
    23341 tatatttttg atcttttagc ctccggacta gtataaaccc caatgacccg gtaactaaat
    23401 ccattgactt ctacaacctt gttaatagcc tcttgaggag attcaaataa actaatggac
    23461 aattcctcat ctagcaaaat gacacttgca aactctttga aatcttgctc tctcagacta
    23521 cgacctgcaa taatttcatt cttaacagcg tccatgtaag ttctgtttcc acctgtcaaa
    23581 ttagcattct caaccttttt atcttgatag gtcaagatgg cattcgttga attggttaca
    23641 tagtaactat ccactccctt cagtttagct gcctcttgaa cccaggattc ttgcggtttt
    23701 ggcggttcaa caggaacttc ctcttccttt ccagaaaccg taaaagctga ttgtttctga
    23761 gtaaaagacc catctttact ttttttagga gagaaaaaga cgctaatatt tttctgagat
    23821 ttagtcatat ctttattgac ttgacgagat agggaatcac ccaaagccat aatcacaaca
    23881 actgatgaaa caccgataat aatcccaatc atagtaagca aagaacgcat cttgtgagcc
    23941 atgatagatg aaaaggcaaa tttcagattc tgcatcttag ttttcctcct ttcctaactg
    24001 agcactgtca gacgaaatga ccccatcccg aatgacaatc tgacgtttgg cataggcagc
    24061 aatctcaggc tcatgcgtta ccatgataat ggtttttcct tctttattca aatcaaccaa
    24121 taattgcata atttggttac ctgttttggt atccaaggct cctgtcggtt catccgctag
    24181 gataatagaa ggattgttta ccaaggcacg cgcaatggct acacgttgct tttgaccacc
    24241 agataattct gaaggtaaat ggtgactacg ttctgtcaat tcaaccttgt ctaaatattc
    24301 ctcagccaac ttgcgacgtt ttgaagacga aactcctgcg taaatcaagg gcaattctac
    24361 attttgcaga gcattgagct tcgatagaag aaagaactgc tgaaagacaa aaccgatttg
    24421 ttggttacgg accttagcca gttgtttttc accaagccca gccacttctt gaccttcaag
    24481 ataatattct ccactggttg gtgtatccaa catgccaatc gtattcatca gagtggactt
    24541 accagaccca gatggtccca tgatggctac aaattcaccc tcattcactt ctagattgat
    24601 atttttgaga acctgcaatt cttggtcacc attacggtag cttctgaaga tattttttag
    24661 actaattagt tgcttcatca gccttcacct cttttccttc ttccaaggaa gatgttggat
    24721 tactgatgac cttagcaccg ttcgttaaac cagaagtgat ttcttgattt tctgcgtcag
    24781 catttcccaa tgaaacctca acttttttag ccttttgttg ttcatccaca atccagacat
    24841 aatttttact atcatccatt actagactgc taacaggaac aagaatagcc ttagttttgc
    24901 ttttaacctc aatgttgaca gaaaaacctt gtttcaaatc accaacctcg cctgtcacat
    24961 caatagtata agggtattta gaacctgtat tattcccggc tgctggacta gctgcttcac
    25021 cattgttttt aggatagtca gaaatatagc ttaatttccc agtccatttt ttatcaggat
    25081 acactttaga agtaaagctt acttcttgac ctacagaaag gttggctaga ttgtactcag
    25141 acaattctcc cttgacttgt aaattttcat tgctgacaat atgaaccata acttggctcg
    25201 cccctgttgg agatttagaa acattgctat tgacttcgac tacagttccc tctagggtac
    25261 tgagaacagt tgttgcatcc aattgacttt gagccttact taattgcgcc gcagcatctg
    25321 cacgcgcatc acgggcatca cccaattgag cgtcaataga agcaacagaa tttccagcca
    25381 ctggagttgg gctttgcacc gttgcatctt ctcctcctac tggcgctggt aactgtggag
    25441 ccggagctga agcggcttca tttcgtgctt gattgagttc attgatatga cgatctgcct
    25501 tagctactgc tcgactagct gaatcatagg ccgcctgcgc ttctgaacta ctgtacttga
    25561 ctaaagcctg cccttcgctg accttatcgc ccacagaaac aaggatttca tctaaatcac
    25621 ccttactagc atcaaaataa acatattgtt cattttttgc tgttactgtc cctgacaata
    25681 aaacagagga tgccacgctt ccttccttgg caacaacaag atgagtaggc tcatctttta
    25741 gagcagtctg agaaggttgt ctaaagagta aaatcccccc ggcacccaat acaactacac
    25801 tcgcagcacc gattgctgca tacagttgcc actttttagc tttaccattc tttttcttca
    25861 taatgaaact ccttttcttt tttacaatac tttgctatta taccaaattt ccctccagca
    25921 aacaatgcag ttcagatgga aataatggca gttcaggatt aaacaatcgt tcggaatttt
    25981 gcttttctat tgtactagat atataataca ctatcttgac ttatttggca agcttttttg
    26041 caattttttg tgcgtagcag atttttgcaa tcaaatcaaa aaaagaataa aatataacta
    26101 tcaaaaaaga cactctacta tttaaaagat tacaaaggag ttttcaatga gagaatatga
    26161 tatcattgct atcggtggag gtagcggagg aatcgctacc atgaaccgtg ctggtgaaca
    26221 tggagccaaa gcagccgtta ttgaggaaaa gaaattaggt ggaacctgtg tcaacgtcgg
    26281 ttgtgttcct aaaaaaatca tgtggtacgg ggcgcaaatc gctgagactt tccatcaatt
    26341 tggagaagac tacggcttta agactactga tcttaacttt aactttgcaa ccctacgtcg
    26401 caatcgtgaa gcctacattg atcgcgctcg ttcttcttat gatggtagtt ttaaacgcaa
    26461 cggtgtagac ttgattgaag gtcatgctga atttgtagat tctcatactg taagcgtaaa
    26521 tggtgaactg attcgtgcta aacatatcgt gattgctaca ggtgcccatc caagtattcc
    26581 taatattcct ggtgctgagc taggtggctc ttctgatgat gtatttgcct gggaagaact
    26641 tccagagtca attgccattc taggcgctgg ttatatcgcc gttgaattag ctggcgtact
    26701 ccacactttt ggtgtcaaga cagatctctt tgttcgccgc gatcgtcctt tacgtggttt
    26761 tgattcctac atcgttgaag gtttggtcaa ggaaatggaa agaacaaact taccacttca
    26821 cactcacaaa gtccctgtca agttagaaaa aactactgac ggcattacca ttcatttcga
    26881 agatggtact agtcacacag ctagccaagt tatctgggct acaggtcgcc gtccaaacgt
    26941 taagggcttg caacttgaaa aagctggagt gactctgaac gaacgtggct ttatccaagt
    27001 ggatgaatac caaaatactg ttgttgaggg aatctatgct ctaggtgatg taacgggcga
    27061 gaaagaactg actccagttg caatcaaggc cggacgtacc ctatctgaac gtctctttaa
    27121 cggaaaaact actgcaaaaa tggattactc aactattcca actgttgtct tttcacaccc
    27181 tgctatcgga actgttggtt tgacagaaga gcaagctatt aaagaatacg gtcaagacca
    27241 aatcaaggtt tataaatcaa gctttgcatc tatgtactct gcttgcactc gcaaccgtca
    27301 agaatcccgt ttcaaactca taacagctgg ttcagaagaa aaagttgtcg gacttcatgg
    27361 aattggctac ggcgttgatg aaatgattca gggatttgcc gttgctatca aaatgggagc
    27421 aaccaaggct gactttgatg caactgtggc aattcaccca actgcatctg aagaatttgt
    27481 aaccatgcgt taatcgaaac aaaagaagct gatataaata tgtctgcttc ttttttaatg
    27541 attttcgaat caacaaaaaa attatccaac ttgactagtc acgaaaatcg gctgttacag
    27601 tttttgttac cttgttttta aaaaatggtt gacatcgact cctatatgag tataatagtt
    27661 actatacatt agaaaagagg ttaactattt tgaaaaaagc tcacgtttat gctatccctg
    27721 ctattggggc tgctctcatt gctgttttgg cacaaatcag tcttccaatt ggacctgttc
    27781 ccttcactct gcaaaacttt gcaatcggct tgattgctac tgtctttaga ccgagagagg
    27841 ctgtactttc tgctggactc tatcttcttc taggtgctat cggtcttcct gtctttgcag
    27901 gaggtggagc tggttttcag gctttagttg gccctactgc aggctatctt tggttttatc
    27961 tcgtttactc tggacttact tcctctctaa ccaacagcaa gagtggtgtt gttaagattt
    28021 ttcttgcaaa cctcttgggt gatgcccttg tctttgtcgg cgggattctc agcttgcatt
    28081 tcctagctgg aatggcattt gaaaaagctc ttgttgtggg ggttcttccc tttatcattc
    28141 cagaccttgg caaacttctg gctattagtt ttattagccg tcccctactt caacgcctta
    28201 aaaatcaggc ttactttact aactaaaaaa ggataccgag ttatcatgac tcaatatcct
    28261 tttttttatt ttgaaaactt atactcaatg aaaatcaaag agcaaactag gaagctagcc
    28321 gcaggctgct caaaacactg ttttgaggtt gtagatgaaa ctgacgaagt cagtaaccat
    28381 acggcaaggc aaagctgatg cggtttgaag agattttcga agagtattag ttgcctttaa
    28441 aggcatccac catcgtttga aattcttcat ttgagagagt aatccctttg cccattttag
    28501 tatggtctgg actccaagca cgaatatcaa actttgcagg ggcaccatta aagctcacac
    28561 ggttaatttc cttggtccaa cctttttcgt tttcagaaag agtcaacaag tgctcttcga
    28621 tttcaaatgt aaattctgcc attttcttct ccttttttag tttcattagt ttattcgtaa
    28681 aatcttgtag attttaggaa aattttatat aatattgata taaaagaagg gaggccaata
    28741 tgagacataa attccagcaa gttctagata aaatacatga ttttttaaat ggacatgacc
    28801 aacctgacca gactgaaacc aactccctta cagccactat tgaagaggct atccagaaac
    28861 aaaccgctgt tcaccttatc ttgtctgaga caagctttac aggtgatatc atcaaatatg
    28921 atcagcaagg ccagcaaatt atcgtgaaaa attttgccaa aaatgtgagc cggattatcc
    28981 gtataagcga tattcaacgc ctgcgatttg tcccctcaac tgtccaaaca gcccaaaaaa
    29041 atagatttaa gaaagagtga gatgtagttg cttcatccca ctcttttttc ttagcgaatt
    29101 tgttcaaaat gtaaatgaac tgcgatatga tctccataac cacttctttc caagtcacgt
    29161 tgtaaacgat aggaaatgta gtgttctgca atggtaatgt aacctgcgcc caataaacga
    29221 tgttcaacca tagattgaat catactgata gtcgcacgtt ccaccttggc ttcttgtaaa
    29281 tccaaaacta ccttcttagt gacttgagca agattttgac gcaaatcatc tgtcaaaaca
    29341 taaacagttt gggctgcctt caagatggct tggtaaatct tatctggatt aaattcagca
    29401 atttcgccat tacgtttgat tacttgcata ggtttctcct ttattctttg ttttctttga
    29461 tttctgccag cattttttct tcttctactg tcagttgata atgttcaagt aaatccggtc
    29521 tgcgctcgta ggttttcttt aaactctcgt acaatcgcca ctgacgaatc ttttcatggt
    29581 ggccactcat caatacatct ggcacgacca tgcctcgata atcataggga cgtgtgtact
    29641 gaggatattc taaaagacct gaagaaaaac tatcatcttg gtggctagac tccttaccaa
    29701 tcacttctgg aatcaggcga actgtagcat caatcatggt catagctgcc aattctccac
    29761 cagtgaggac atagtcgcct agggaaatct catctgttac caaggtctta atgcgctcat
    29821 cataaccctc atagtgccca cagataaaga ttagctcttc ctcttgagcc aaatcttcag
    29881 cataagcctg atcaaactgc tttccagcag gatcgaggag aataacgcgc ggatttttct
    29941 tttcaatagc atcaaaggaa tcgaaaatag gttgtgctct gagcaacatg ccctgaccgc
    30001 ctccgtaggg ctcatcatct acatgacggg ccttttcagc attttctcga aaattatgat
    30061 actggatatc caagagccct ttttctcgag cctttccaac gattgagtgc tccagtggag
    30121 aaaacatctc tggaaagagg gttaaaatat caatcttcat cgtctaaccc ttctaagatt
    30181 tccacatcga cccgtttatt tggaatatca acattgagaa ccactggtgg gatataaggt
    30241 aaaagcaaat cacgtttgcc ttttcgtttg accacccaga catcattagc acctggttgc
    30301 aggatttcct tgatggttcc aaccaagcta tcaccctcat agacttccaa accgataatc
    30361 tcgtgatagt aaaattcacc atcgtctagg tcattcaaat cttcctcagc gaccttgaga
    30421 ctgtatccct tgtacttttc gatagtattg atatggtaca tatctttgaa tttaataatg
    30481 tcaaagttct tctgtttacg gtggctagcg atggtcactg tttggacaaa ctgatctttt
    30541 tcatcaaaca aagtcagctc agctcctttt ttaaaccgtt cttctgcaaa atccgtcaca
    30601 gacaatactc gcatctcacc ctgtaatccc tgcgtattaa cgattttccc aacattaaag
    30661 tagttcatct tgtctcctgt aatctccttt ttccatctta ttctgacaat tctcgaataa
    30721 tagccgcaat tttttccgat tctgaccatt gtaaataatg gtgattcctt cctaaaatga
    30781 gtttagtatt ggaagtccaa tattctgatt ctctgtactc tttttctcta taagtctgac
    30841 aaaaaataaa tacagggaca tgagattcta tcgacaaatt ctcaaaatct tcctcagtaa
    30901 tctctccaga tatctgaaat tctggatctt gattttccaa ctctaagcct ttttcttgca
    30961 ttaattccca gattttttta ttcgtttcag gactaaatgt tgcttgagtt aagttcttaa
    31021 aataaagttc aggaccacac tcgtcaatca gcctcatctg ctcttccatt tctggataag
    31081 gattttctga aaaatcagca aacatgactt ttttagttgt cggttcaatt gctactaaag
    31141 tctgacactt aattggtttc tcgagtaatt tgcaagctaa aattccactc caactatgtg
    31201 cacaaagtat atattcagaa attcctaatt cttcaagtac ttcataaacc gcatctgcaa
    31261 gattatctag attttttcca gcttggtcat gaatcggact cctacctgtg ttcggaaaat
    31321 caattgtcaa ataaccaatt gtaggaggag gtttttcaag tataagtgaa aaattttcat
    31381 aacttggtag caaacctgct ccgtttagac aaactagcac tttcttttgc tttttataag
    31441 taacagagag gctaccaatt tctgtagata cttcaaacct cttcataaag aaatccactc
    31501 attctatata atgaattatt aaaaatcctt atcctttatt ttatcacgtt ccaaagattt
    31561 tctcaagttg gaggaagggg acaatatctc tactttccct tcaagaatcc ttccaaatca
    31621 tgtttatgtt ggtaattaac ggctgcggtt ttgtctttct caaagatggt tctagttagg
    31681 tcaatatgct tgatagcagc aatagcgacg gtgtagtgaa tgatattagc cagttctatg
    31741 gcaggttcct cgttcgaatc ctatcccttc ttttcgacca gagcgcctat tcaaaacctc
    31801 ggctacttct ccgacttcct ccactaactt cataaagaga ccttcatcag tccgagactg
    31861 ctgttaatgt tcgagtaagt agtcttggaa ttgcctaaac gttcaatctt ttatagtata
    31921 ttgaaactag aatagtacac ctttacttct aaaacattgt tagaaatcga tttgactgtc
    31981 ctgatcgatt tgtcctgtta ttatttcatt ttactatatc ttctattcca cacaaaaaag
    32041 cgagacatcc gtcccgccct tcttattttt cgtcaataac gattcttact tttttgtatt
    32101 cagttgggac agagtagaca atcgttctta tcgcagaaat agtgcgaccc ttacgaccga
    32161 ttacacgacc cacatcgctt tgatcaagat tcaaatgata ttccaaaaat tctggtgtat
    32221 cctcaatctt gatagttaag gcatctggtt gtgaaattaa gggtttcaca atcgcaataa
    32281 tgagattttc aatcgtatcc atctgtcaac ctactttaaa cttattttga gaatttagaa
    32341 tcgtggaatt ttttcaatac gccttctttt gaaaggatgt tacgtactgt gtctgaaggt
    32401 tgagctccat tagccaacca tgcaagaacg cggtcttctt tcaaagttac ttggttttca
    32461 gcaacaagtg ggttgtaagt tccaactgtt tcgatgaaac gtccgtcacg tggtgaacgt
    32521 gaatctgcta cgttgatacg gtagaaaggt tttttcttag aacccatacg agtcaaacgg
    32581 attttaactg ccatttttaa agtctcattt ctttaatttt ttatttcggt gaaatagctg
    32641 agctatttag cacatgttct attatagcag atttctggca ggtgtcaaga aaaaaacttg
    32701 acagactata aatttcttaa agcatttaga aatttgttag aataggaaat aa
//
LOCUS       DAVLXF010000011        31944 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLXF010000011 DAVLXF010000000
VERSION     DAVLXF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.011"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(778..1191)
                     /gene="ndk"
                     /locus_tag="VYO12_000489"
     CDS             complement(778..1191)
                     /gene="ndk"
                     /locus_tag="VYO12_000489"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1587142.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(1302..4979)
                     /gene="rpoC"
                     /locus_tag="VYO12_000490"
     CDS             complement(1302..4979)
                     /gene="rpoC"
                     /locus_tag="VYO12_000490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1587143.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(5012..8623)
                     /gene="rpoB"
                     /locus_tag="VYO12_000491"
     CDS             complement(5012..8623)
                     /gene="rpoB"
                     /locus_tag="VYO12_000491"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1587144.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            9039..9109
                     /locus_tag="VYO12_000492"
     tRNA            9039..9109
                     /locus_tag="VYO12_000492"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9071..9073,aa:Cys,seq:gca)
     gene            complement(9307..10638)
                     /locus_tag="VYO12_000493"
     CDS             complement(9307..10638)
                     /locus_tag="VYO12_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1587145.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(10929..11753)
                     /gene="endA"
                     /locus_tag="VYO12_000494"
     CDS             complement(10929..11753)
                     /gene="endA"
                     /locus_tag="VYO12_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1587146.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11792..11980)
                     /locus_tag="VYO12_000495"
     CDS             complement(11792..11980)
                     /locus_tag="VYO12_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1587147.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11973..13256)
                     /gene="murA"
                     /locus_tag="VYO12_000496"
     CDS             complement(11973..13256)
                     /gene="murA"
                     /locus_tag="VYO12_000496"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1587148.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(13280..13510)
                     /locus_tag="VYO12_000497"
     CDS             complement(13280..13510)
                     /locus_tag="VYO12_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1587149.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(13568..14605)
                     /locus_tag="VYO12_000498"
     CDS             complement(13568..14605)
                     /locus_tag="VYO12_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1587150.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     INDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(14589..15077)
                     /gene="coaD"
                     /locus_tag="VYO12_000499"
     CDS             complement(14589..15077)
                     /gene="coaD"
                     /locus_tag="VYO12_000499"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1587151.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPIENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(15067..15606)
                     /gene="rsmD"
                     /locus_tag="VYO12_000500"
     CDS             complement(15067..15606)
                     /gene="rsmD"
                     /locus_tag="VYO12_000500"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1587152.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQITKEIGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYTKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(15671..16663)
                     /locus_tag="VYO12_000501"
     CDS             complement(15671..16663)
                     /locus_tag="VYO12_000501"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1587153.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(16774..17457)
                     /locus_tag="VYO12_000502"
     CDS             complement(16774..17457)
                     /locus_tag="VYO12_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1587154.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(17513..18253)
                     /locus_tag="VYO12_000503"
     CDS             complement(17513..18253)
                     /locus_tag="VYO12_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1587155.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQREEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18287..18565
                     /locus_tag="VYO12_000504"
     CDS             18287..18565
                     /locus_tag="VYO12_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1587156.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            18643..19569
                     /gene="yidC"
                     /locus_tag="VYO12_000505"
     CDS             18643..19569
                     /gene="yidC"
                     /locus_tag="VYO12_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1587157.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(19644..20438)
                     /gene="pflA"
                     /locus_tag="VYO12_000506"
     CDS             complement(19644..20438)
                     /gene="pflA"
                     /locus_tag="VYO12_000506"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1587158.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(20564..21814)
                     /locus_tag="VYO12_000507"
     CDS             complement(20564..21814)
                     /locus_tag="VYO12_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1587159.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21881..22708)
                     /gene="purR"
                     /locus_tag="VYO12_000508"
     CDS             complement(21881..22708)
                     /gene="purR"
                     /locus_tag="VYO12_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1587160.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22975..23916)
                     /locus_tag="VYO12_000509"
     CDS             complement(22975..23916)
                     /locus_tag="VYO12_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1587161.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(23906..25162)
                     /locus_tag="VYO12_000510"
     CDS             complement(23906..25162)
                     /locus_tag="VYO12_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1587162.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(25164..25826)
                     /locus_tag="VYO12_000511"
     CDS             complement(25164..25826)
                     /locus_tag="VYO12_000511"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1587163.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAEV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25789..26445)
                     /locus_tag="VYO12_000512"
     CDS             complement(25789..26445)
                     /locus_tag="VYO12_000512"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1587164.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(26456..27334)
                     /gene="rsgA"
                     /locus_tag="VYO12_000513"
     CDS             complement(26456..27334)
                     /gene="rsgA"
                     /locus_tag="VYO12_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1587165.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(27336..28208)
                     /gene="rsmA"
                     /locus_tag="VYO12_000514"
     CDS             complement(27336..28208)
                     /gene="rsmA"
                     /locus_tag="VYO12_000514"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1587166.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILVDTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(28594..28740)
                     /locus_tag="VYO12_000515"
     CDS             complement(28594..28740)
                     /locus_tag="VYO12_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587167.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDKNMTNHLLLVQILVHGYLLDFA
                     SIEG"
     gene            complement(28733..29374)
                     /locus_tag="VYO12_000516"
     CDS             complement(28733..29374)
                     /locus_tag="VYO12_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587168.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(29376..31406)
                     /locus_tag="VYO12_000517"
     CDS             complement(29376..31406)
                     /locus_tag="VYO12_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1587169.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(31483..31779)
                     /locus_tag="VYO12_000518"
     CDS             complement(31483..31779)
                     /locus_tag="VYO12_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1587170.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
BASE COUNT         9001 a         7439 c         6238 g         9266 t
ORIGIN      
        1 tgagtttcct ttctttttgt gtttttttct acacttatac cataaagggg aaactctttt
       61 ttgtctagta aaaaacaccc attgggtgaa aaaagaaacc atccaggatc taagctaagg
      121 caaggattct ggatggtttt tagatttggg gtgaataatt gggggttatt ccatgttatt
      181 ttgaggaaga tattgtgaaa gtcaaagatg atgataaagt tgagtggaat ttgttagaat
      241 ttcaaaaatt tagagcattt ttgacttagt aatctgtgtt gaaggctcaa aacctatggt
      301 aaaaaagtaa ctttgaaaac gtattgcctc caaagattta gttaaataat gatttaacac
      361 aaaaagaaat tattgaagtt ctggaaagat gttgtttcag tattgagaaa aggtgggaaa
      421 aacttgcgat tttcacagag aaaggaagaa aaagtataga aatatagtca attgaaacaa
      481 gaacaggata aaagaacctt ttgtgccata tttttctcct ttcgctttac aattggattg
      541 aacaccttta ttgtatcgcg tttggagttt ttttggtata accttcgacg cgcacccgca
      601 tagcgggtgt ttttttgtct cgcacctaac ggagcgagac gaactaatag tcacttaatc
      661 aaaaaacgca ccatatcaaa aactaaaaag tttgatatca tgcgtcatgt cttaaactaa
      721 ttgactatac tttctattca aatgagcttt taaccaattg attgagccaa tccactctta
      781 aaaccaaaga gcaatttctc gcttagctga ctcttctgaa tctgaaccat gtacaacatt
      841 ttggataacc tcattttctc cagcagcttt tgcaaagtca cctcgaatag tgcctggtaa
      901 agcttcttct ggacgagttg cacccatcat ggtccgccaa gtttcgatta ctttgggacc
      961 agaaatgaca cccacaagaa ctggacctga agtcatgaat tcacgaatcg gtgggtaaaa
     1021 actctgacca accaagtcct gatagtgctg gtcaatcaac tcttctgaaa cctgtgaacg
     1081 aaactccaat ttttcgattg taaatccacg ttgttcgatg cgctttaaca cttcacccac
     1141 tagccctctt tttacaccat ctggtttgat gataaagaat gtttgttcca tacccgtctc
     1201 ctttgtcagc ttctttcttt tattttacca catttcgtgg aaaaatggag aaagttttca
     1261 gaagagagaa tgagagaacc cgagggttct ctcattctct cttattctac tgtttcttcc
     1321 acagtttcaa cggcagtatc cacaactact tctgttgttt cttcatttcc ttcttcctct
     1381 actggaggat taaggtattc ttcttcgttg acagcatgtg gttcaaggtt acggtaacgg
     1441 gccataccag taccagctgg gatgatctta ccgatgataa cattttcttt aagtccaagg
     1501 agatggtctt tcttaccacg gatagctgcg tcagtaagga cacgagttgt ttcctggaag
     1561 gaagccgctg acaagaaact gtttgtttca agtgaggctt tggtaattcc cataaggact
     1621 gggcgacctg tcgctggaac tccacctgcg ataaggacat ctttgttggc atctgtaaag
     1681 tcattgatat ccatgagggt acccatgaga agatctgtat cacctggatc catgacacgg
     1741 actttacgga tcatttgacg aaccattacc tcgatgtgtt tgtcaccgat ttctacccct
     1801 tggctacggt aaactttttg tacttcaccg agaaggtacg tttcaactga caagacatca
     1861 cgaactgcaa ggagacgttt tggttggata gaaccttctg tcagagcagc accacgcgct
     1921 acttggcccc caacttcgac acgcatacga gctgtaaatg gaacgacata ttcaccttcg
     1981 ccagtttcac ccttaacaaa gactttcttg gtacgagttg atgcatcctc ttcgatagca
     2041 gtaacttgtc ctttaacctc tgtgataacc gcttcccctt taggattgcg ggcttcaaag
     2101 atttcttgga cacgaggaag accctgagtg atatcggtat ttgaggcaac cccacctgtg
     2161 tggaaggtac gcattgtaag ctgtgtacca ggttccccga tagattgggc agcgattgta
     2221 ccaactgctt caccaacttc aaccgcatca ccagtcgcca agttgatacc gtaacagtga
     2281 cggcagacac cgtgacgagt gttacatgta aatacagaac ggatagtcac ttcttccaca
     2341 ccagcattga caatttcacg cgccttgtct tctgtaatca attcatttgg accaataatc
     2401 actgcaccag tttctggatg tttaacagtt ttcttagtgt aacgaccgtt gagacgctct
     2461 tcgagagact cgatcatctc ttttccttct gcgatagaac ggatcaagag accacggtca
     2521 gttccacagt cgtcctcacg gatgataacg tcttgggcaa cgtcgaccaa acgacgagtc
     2581 aagtaacctg agtcggctgt cttaagggcc gtatcggtca tacctttacg agcaccgtga
     2641 gttgagaaga acatttccaa taccgacaaa ccttcgcgga agtttgaaag gattggcaat
     2701 tccatgatac gtccattcgg agcagccatc agaccacgca taccggcaag ctgtgagaag
     2761 tttgagatgt taccacgggc tccagagtcc atcatcataa cgattgggtt cttaggatct
     2821 tgattagcaa tcaagcgttt ctcaagtttt tcacgggcag cacgccattc agctgtaaca
     2881 gcattgtaac gctcgtcgtc tgtgatcata ccacgacgga attgtttggt gatttgttcg
     2941 acacgtttgt gtgattcttc aatgatttca gccttgtcat caacgactgg gatatcggca
     3001 atacccactg tcaatcctgc aagagttgag tggtggtaac cgaggttctt catgcggtca
     3061 agtagggcag aagtttctgt cgtacggaaa cgtttgaaga tttcagcgat gatatttccg
     3121 aggtttttct tcttgaatgg agggttgagc tcaagattgc tgatagcttc cttgatatct
     3181 ccaccaagtg gcaagaagta tttagctgga acaccttctg tcaagttggc attgtttggt
     3241 tcttgcaagt atggtagccc ctctggcatg atatcgttga agagaatttt accaactgtt
     3301 gtaagcaaga ccttatgtct ttgctcttct gtccaaggct tgttgaggct gtctgttgcg
     3361 ataccaacac gtgagtggag gtgaacataa ccattgcggt aagccataac cgcttcgtca
     3421 cggtctttga agaccattcc ttcaccttcg cgaccagctt cttccatggt caagtagtag
     3481 ttacccaaaa ccatgtcctg agatggagta actaccggtt tcccatcttt cgggttcaag
     3541 atgtgctcag cagctagcat gaggatacga gcttctgctt gtgcttcttc tgaaagtggt
     3601 acgtggatgg ccatttggtc cccgtcaaag tcagcattgt aggcttcaca gacaagtggg
     3661 tgcaagcgaa gagccttacc atcaatcaag actggctcga aggcttggat acccaaacgg
     3721 tgaagggtcg gtgcgcggtt caaaagcact gggtgttctt taatcacttc ttcaaggata
     3781 tcccagatac gctcatctcc gcgttccacc aagcgtttag ctgctttgac gttttgcacg
     3841 atatcacggg caacgatttc acgcatgaca aatggtttaa agagttcaat cgccatttca
     3901 cgtggaacac cacattggta catcttaaga gttggaccaa cggcgataac ggaacgtcct
     3961 gagaagtcaa cacgtttacc gagcaagttt tgacggaagc gtccctgttt acctttaagc
     4021 atgtggctca atgatttcaa tggacggcta cctggtcctg tgattggacg accacgacga
     4081 ccattgtcaa tcaaagcgtc aactgcttct tgaagcatac gcttctcatt ttgaacgatg
     4141 atacctggtg catttaactc aagcaaacga gccaaacggt tgttacggtt gataacacgg
     4201 cggtaaaggt cattcaagtc agatgaggca aaacggccac catccaactg caacattgga
     4261 cgaagatctg gtgggataac cggaaggatg ttaagaatca tccattcagg tttgtttcca
     4321 gacttgtaaa aggcatccaa aacatccaaa cgacggatgg ctttgacacg tttttgtcca
     4381 gtagctgttt tcaattcttc tttgagttca gcaatttctt tttcaagatc tacttgcttc
     4441 aaaaggtctt ggatggcttc cgcacccatc ttggcaacaa atgaaccata accatattca
     4501 cgcaagcgct ctcggtattc gcgctctgtc atgatagact tgtgctcaag tggtgtatcc
     4561 ttaggatcaa tcaccacata agccgcaaag tagataactt cctcgagggc acgagggctc
     4621 atatcaaggg tcaagcccat acggcttgga atccccttga agtaccagat gtgagataca
     4681 ggagctttca attcgatatg tcccatacgc tcacgacgaa ctttcgtacg cgttacttca
     4741 accccacagc ggtcacaaac aattcctctg taacgaatgc gtttgtactt accacaagca
     4801 cattcccagt cttttgtagg accaaagatc acttcatcaa agagtccttc acgttctggt
     4861 ttcaaggtcc gataattgat tgtttcaggt tttttgactt ctccataaga ccatgaacgg
     4921 actttacttg gagaagctag ggtgatttgc atacttttaa aacgatttac atcaaccact
     4981 atttcttccc tttctattct aagtgaactg cttattcttg ttcagcagct tcttctgttg
     5041 cttccgcttt tgttgctttc tcagcttctt cagcttcaaa ggctgcttta gcctcttggg
     5101 ctgctttttc gcgggctttt tcaaggtcat ctacgtggat gacatcttcg tccattcctt
     5161 catccaagtc gcgaagttcc acttcttggt catcttcgtc taggacacgc atgtcaagac
     5221 caagagattg caattctttg acaagaactc ggaaggattc tggaacacct ggttttggaa
     5281 ttggtttgcc ttttgtaata gcttcatagg ctttcaaacg tccgttgata tcgtccgact
     5341 tgtaagtcaa gatttcttga aggacatttg acgcaccgta ggcttcaaga gcccaaacct
     5401 ccatctcacc gaaacgttgt ccaccaaact gagctttacc tccgagtggt tgttgggtaa
     5461 cagttgagta aggtccgact gaacgcgcat gcaatttatc gtcaaccatg tggtggagtt
     5521 taatcatgta catgactcca acagaaacac ggttatcaaa tggttcacct gtacgtccat
     5581 cgtaaaggat tgtcttggca tcgctatcca tacctgcttc tttaacagtt gaccaaagat
     5641 cttcagagct tgctccatca aagactggtg tcgcaatgtg aataccaaga gtacgagctg
     5701 ccataccaag gtgaagctcc ataacctgac cgatattcat acgtgatggc accccaagtg
     5761 ggttcaacat gatgtcgact ggagttccgt ctggaaggta aggcatgtct tctacaggaa
     5821 cgatacgaga gacaacccct ttgtttccgt gacgtccggc cattttatct ccgaccttaa
     5881 tcttacgttt ttgagcgatg taaacacgaa ccaacatgtt aacacctgat tgcaactcat
     5941 ctccatttac acgtgtaaag atcttaacat cacgaacgac accatcggca ccgtgtggta
     6001 cacgaagaga agtatcacgc acttcacgag acttgtctcc aaagatagcg tgcaagagac
     6061 gttcttcagc tgaaagatct ttctcaccct taggtgttac tttacctaca agaatatcac
     6121 cttctttaac ctcagcacca atacggataa tccccatttc gtcaaggtct ttgagggcat
     6181 cttcaccaac gtttggaatt tcgcgagtga tttcttcagg cccaagcttt gtatcgcgcg
     6241 tttctgattc gtattcttca aggtgaacag atgtgtagac atcgtccttc accaagcgtt
     6301 cgctcatgat aacggcatcc tcgaagttgt aaccttccca agtcatgtag gcaacgattg
     6361 ggttttgtcc aagcgccatt tctccatttt ccatagaagg tccgtcagcg atgaaatcgc
     6421 ctttttcaac gacatcacca acttttacga gagtgcgttg gttgtaagca gtacctgagt
     6481 ttgaacgacg gaatttttgg atgtggtaaa catccaatga accatcttca cgacgaactt
     6541 ctaccttgtc agcatctgcg taagtaactt taccatcata ctgagcaatc acagccgcac
     6601 cagaatcgtg ggctgcttgg tattccatac cagtaccaac gtaaggtgcc tgaggattaa
     6661 tcaatggcac agcctgacgt tgcatattgg ctcccatgag ggcacggttg gagtcatcgt
     6721 tttccaagaa aggaatacat gctgtcgcaa cggcaactac ctgttttggt gaaacgtcca
     6781 tgtagtcaac aatattagct ggatactctt ggttgacccc ttggtgacgt cccatgacaa
     6841 tcttctcagc aaaggttcca tcttcattca gacgagagtt agcctgagct acagtatatt
     6901 catcttcttc atcagctgtc aaccaaacaa tttcgttcgt gacaacacct gtttcacggt
     6961 caaccttacg gtatggtgtt tgaacaaaac catatttgtt caagtgtccg taagatgaca
     7021 agttattgat caaaccgatg ttaggtcctt caggtgtctc gattggacac atacgaccat
     7081 agtgagtgta gtgcacgtca cgtacttcat atccggcacg gtcacgagtc aaaccaccag
     7141 gtcctaaggc tgacaaacgg cgtttgtgag acaactcaga aagcgggttg tgttggtcca
     7201 tgaactgtga caactgtgat gaaccaaaga attctttaac tgcagctgtt acaggacgga
     7261 tattgataat ttgttgtggt gtcaagactt cattgtcctg aacagacata cgttcacgga
     7321 cattacgttc catacgagaa agtcccaaac gtacttggtt ggcaagcaat tcaccaaccg
     7381 cacggatacg acgatttcca aggtggtcga tatcatctac acggccaagt ccttcagcca
     7441 agttgaggaa gtagctcatc tcagcaagga tatctgcagg agtcaccgta cgaaccttgt
     7501 catctgggtt agcattacca atgatcgtta cgacgcgatc tggatcagtt ggagcaataa
     7561 ccttgaattt ttgaagaaca acaggctcag tcacaacggc tgcatcgttt gggatgtaga
     7621 caatcttgtt caagtcgcca tccaaatggc tttcaatgct ttcaatcacg ctacgagtca
     7681 taatcgtacc agcttctacc aagatttctc cagtttcagg gtctaccaat ggctctgcaa
     7741 tggtttggtt gagcaaacgt gttttaacat tgagtttttt attgattttg taacgaccaa
     7801 ctgctgccaa gtcataacga cgtgggtcaa agaagcgagc tacaagcaag ctacgtgagc
     7861 tttcagccgt cttaggctca cctggacgaa ggcgttcgta aatttctttc aaggcttcgt
     7921 ctgtacgaga gtccattgga ttcttgtgga tatctttttc aacagtgttg cgaaccaatt
     7981 cgctgtctcc aaagatatca aagatttcat catcacctga gaaaccaaga gcacgaacca
     8041 aggttgtaaa tggaatctta cgagtacggt cgatacgagt gtaggtgata tcttttgagt
     8101 cgctttcaag ttccaaccaa gctccacggt tagggataac agttgaacca tagcccacct
     8161 taccattttt gtctactttg tcgttaaagt aaacacctgg tgagcggacc aactgagaaa
     8221 cgataatacg ttcaccacca ttgatgatga aagtacccat ttctgtcatg attgggaaat
     8281 caccaaagaa aacttcttgg gtcttgattt cgcctgtttc tttattgatc aaacggaagg
     8341 ttacaaaaat tggtgctgag tagctagcat cgtggatacg agcttcttct agcgtgtatt
     8401 ttggttcctt gatttcatat ccaacaaatt ccaactccat tgtgtctgtg aagtttgaaa
     8461 ttggcaatac atcttcaaac acttccttaa gaccgtggtc taggaaagct ttgaatgagt
     8521 cagtttgaat ttcaatcaaa tttggtaagt caagaacttc tttgattctt gaaaaactac
     8581 gacgggtacg atgtttcccg tattgaacgt catgtcctgc caagatgatt ctcctttgta
     8641 aataagttcc aagccttgtc aatcaggctt ttctaatcgt catatggttg taaacccctt
     8701 atcaccgtgt cctcttgacg aattttcaga atctttaagc ctctgttaca aatgctcata
     8761 atcttgaaaa aaagcacaaa aagagcagct aaatctgact ttttcagaag atttaactgc
     8821 tgtgagcctt gtctggacaa tatttcagac aaaacctacg acaaatgatt acccatatta
     8881 taccctattt agctagattt ttcaaggggt ttcagtaggt ttttggtaat ttttttccca
     8941 tagaaaactt ggcatcacat tcgaatcacg ctatggtaca aaaaactgaa aaaactattg
     9001 actgaaaatc attttcaagg tataataata aacgttaagg cggtatagcc aagtggtaag
     9061 gcacggctct gcaaaagctt gatcgtcggt tcaaatccgt ctaccgcctt ctataacttg
     9121 atttatcagg tttcaaatga acagaaagcc caatttgaag ggcttttttt attttccctc
     9181 gaataaatac gtataacttt aaaaactttt ggagcgagtt tgtggcagag ttctttccat
     9241 ggcataattc ccttttgaaa tcaattgaaa aaagcttcgt cacgcctggt gacaaaagcc
     9301 tctagtttat tctgtttctt cttctatttc gacttctgtg atttggactt ttaccttggt
     9361 aacacgtcca ttcttcactt tatcattggt taagataaac tgtttgtttt ggctgaccaa
     9421 ttcatagctg agtttctcag tcgttggaat ggttcccact cctgtcaaat aataaccagc
     9481 gatggtatca acgtcatcac tttccagctc aacatcaaag taattattga aatcattgag
     9541 ggtcatagtt ccttgaacaa tgtaggtatc ctcgccaatc tgatgaacat ctattgctgc
     9601 tttgtccgtt tcatcatcaa tttcaccaac aatttcctct aagaggtctt ccaaagtcac
     9661 taaaccagcc ataccaccgt attcatccag caagattgcc atttgtcttt gggtatttcg
     9721 cagttctttt agcaagtcat ccacaaaaat agtttcaggt acaaaaagtg gatcttgtaa
     9781 aattctcttc caaacaatat tgtcaaaacc gtccacaaag cctgccttaa ggagactctt
     9841 ggtgtgaatg attccaatta cattgtcctt atccccatca taaaccggga tacgagaata
     9901 attttgtttt aaaatacttt ggataatggc ttgactatca tcctgaatat ccaccataaa
     9961 ggcatccgtt cgaggaacca taacctctcg tgccatcagt tcatcgagcg aaaagacacc
    10021 ttgtagcatc tcaatctcat cagcatccaa tgtttcttca ctatttgtca gcatataggc
    10081 aatttcatca cgggtcattt tttcatccgc atcatcgaat gacataggag tcaaatggct
    10141 caagaaattg gtcgaagcag ctaaaagcca aacaaaagga ctgactagtt ttccgatccc
    10201 aatgataacc ggcgctgtac gaattgccaa ggcatccttt agattaagag cgattctctt
    10261 aggatataat tccccaaaaa cgatggaaat ataggtcaaa aatgccaagg atagaaaagt
    10321 tgccacggct tgtgctgttt cgccattccc aagccaagag gcaatcacac gtcctagagt
    10381 atcagttaaa ctcgcccctg ataagattgt aatcagggtg attcctacct gaatggttga
    10441 taaaaagtgg ttaggatttt ctagtacctt cagcaggcgg atgtagcgtc tgtctccttc
    10501 ttccgccttt tgttcaactc gggcacgatt aagagaaacc atggccattt ctgtggctga
    10561 gaaaaaggca tttaataccg tcaagataaa caataaaaca aattgtagca acaaattctg
    10621 actacttggg tcttccatag ttcttctcct aaaatagtat cttatccttt attatatcac
    10681 aaaatttaat ccaatacata ttattttttt cttagcgtaa cttccatctc tctcccaaaa
    10741 ctggaagtta gtctaagacg gatttccact gatttcacgc gtttttctca tactaaaagc
    10801 taggttgtgg gaaactggaa gttagtttta gaagttacta ttttttctta gtacctattc
    10861 tagccctgct taaactagta aaaagaactc ctacatccat aagtgacata ggagtttagg
    10921 cttatctttt actgagttac agttacttct ccagttcggt aatccagttg aagtcccttt
    10981 tgaacattgg gaactagaac attgaattcc aattctccat ccgaagactt ggcttcaatc
    11041 tgtgaagctg agggaactaa atcctcgttt gaagcgtagt aaagggttac acggtaacgg
    11101 acacgcttgt tttggtccaa ggctttacgc accttgcttt catagtagtt ttgaccagtc
    11161 gaatactcgg cttgtgcctg atttgcccag gctgtctgaa cagcaatgtt tttaggattg
    11221 cttgttgagg catcaaaacc atccaaacca ccgattaagg catagcctaa caaatgacct
    11281 ctatcgactg catgggtata agagcccttt agattcttga cctgatgcca acctggagga
    11341 gtccaagaag ttgaaccatt cccagtttct ttacgattct tgtactgacg agtggcctta
    11401 gacaagaggg cattagctac ggttggaaca gtttccttgc ccactgtctt tgttttattg
    11461 tcagcgtagg gcttacttga aaccttggca tctagatttg ttttattacc attgacgata
    11521 aaagcacctg agccattcca ctccagactc ccctttattt gactcttgac tgcgtctgtt
    11581 aagacactct ctgccaatgc ttgactagga gcttcagacg cttgtttttt ctgactaaga
    11641 ttggttttgg gactattagg tgccgactgc atctgcttga tataatagct ccctgtagac
    11701 aaaagcaata acactagcag tccgattagt gtctgtcttg tttttttgtt catatttctc
    11761 cttatcttta gaaaaaggct ggttctccag cctaatttcc tgtaaattta tgaatcaatt
    11821 cctgccattt tgctggagac aggatagccc atggatcttg acccttgccc aagattccat
    11881 aacctaccat tagaccgatt cctagagcca gagtacctaa aatcagtact atgatgacta
    11941 aaagtaaacg attgattacg tagcttgatt tcttattcat cttcatcgct tgcctcaatc
    12001 cgctgaatct tagcacctag ctgcgccaac ttctcatgga aaccgtagta acctctatcc
    12061 aagtgaacca atttaccgac cacagtttct ccctgtgcta ccaaacctgt caaaatcaag
    12121 gctgcactgg cacgaaggtc agttgaaaga acttctgctc cctgcaaagg ctgtccacca
    12181 acaatacgag ctgtatcacg gataatctca gaatgcaagc ccatgcggcg catctcttct
    12241 aggtgttgga aacgattttc gaaaactgtc tccaccatgg ttgattcgcc ttttgcaact
    12301 gtcatcaagg ctgtaaattg agcctgcata tctgttggaa atcctgggtg gggcaaggtt
    12361 ttcacatgaa cagcttttag attttctagt tgagaacgaa cacgaattcc ttcgtcttct
    12421 tcaattactt caacacccat ttcaagtaac ttggcaatca agggacggtt gtgctcccag
    12481 acagcgtctc gaatcaagac atcaccacca gtcatggcag cagctaccat aaaggttcct
    12541 gcttcgatac ggtcttggac tacattgtga gtcgtaccat gaagtttctc aacaccagta
    12601 atggttatag tctctgtacc agcacctttg accttggctc ccatttcatt aaggagaatg
    12661 gctaagtcaa caatctcagg ctcacgcgca gcattctcaa tcactgtcac cccatcagcc
    12721 agagtcgctg ccatcatcaa gttctgcgtt gcaccaacac ttggaaagtc catatagata
    12781 tgagcaccat gcaagcgttc tgccttggct tcgatgtaac cagctgtctg actaatctta
    12841 acccccatag cttccagacc tttcaaatga agatcaatag gacggctacc aatcgtacaa
    12901 ccacctggca tggatacctt ggcatgaccc acacgggcaa ggattggccc taatacaacg
    12961 atggaggcgc gcatcttgct gacatacttg taaggggctt cctcagtgat gtcgccagta
    13021 gcatccacct tgacaagatg agcttcctca tcaaagtcaa ccttggcatt caaaccacca
    13081 actacctgat tcataataaa gacatccgac aaaatcggaa cattctgcaa gacggtcttt
    13141 ccttcacttg ctagaatagt cgctgccaac aagggtaaga ctgcattttt tgctccctcg
    13201 atcgtcacgc ttcctaccag acgattatcg ccaccttgaa ccacaatttt atccatactc
    13261 gtttccttta ctcttgattt cataataatc ctctaagcgc ttcttgccac aaatgataca
    13321 ggctaataaa gaaagaactc aggatgtagc ctatgacaat gctaaaaaag gctactaata
    13381 aacgaacttt tcttgtattc tcatctgtca cttttaaaac cttctcccat ctaacaagat
    13441 cctttaaaag gtaaaaactc acataaataa agagcatatg actacttaga gtgaataata
    13501 attgaaccat ttgactatta taccatcttc tctgtttgtg cgctagtttg gaaacttcac
    13561 tcaaaaatta gggattgttt ttcaagtaat caatcgcatc ttgtagggtt ttaacgggca
    13621 cgattttcat atccgtcttg attgttttag ccgcttctag ggctgtttgg tagttgtttt
    13681 tcgcgtccgg atgcgccttt tgttcttctt cgctaacagg gttatcagga gcaaagaaaa
    13741 tagcagcacc tgccctagcc gaagctacaa ccttcttatc aatacctcca atgtctccca
    13801 cattaccatc gcggtcaatg gtacctgtac cggcaacaat acgaccatta cgaagatctg
    13861 ggtgagctat ttgagtatag atagctagac taaacatgag accagcactt ggaccgccaa
    13921 taccagctgt tgaaaagcta attgggacat tgctgattac ctctgtacgg tcaatcaagc
    13981 cgattccaat tccatttttg ccattttcca aggtgataat ctttccttct gcagacttgg
    14041 tttgcccatc ctcttcatag gtgaccttga cggaatcccc taatttttga gaactgacgt
    14101 aatcaatcaa gtctttggaa ctatcaaagg tctgatcatt gactgctgtg actgtatcag
    14161 agatattgag aatcccttta aaggttgaat tatccgtcac attcaaaaca taaactccaa
    14221 agtacttgag ttcgatatcc ttaccagctg tttttagtcc ttgatacttg gccatatttt
    14281 gcgatgtttg catgtagaat tgattgattc gcataaattc aacatcggaa gaaccacctg
    14341 tagtctcctg agcactacga atatctgtaa aaggtgtcaa ccaagcataa atcatatgag
    14401 ctaaagtggc atgttgaaca ccaaccgtaa cgaattgata ggcaccagct tccttatctt
    14461 ctgtgtcatt tattttaagg acttggcgaa tatcttccga accacctgga acctctatat
    14521 aataaggcaa gggcactaca aatgccaaga aagtcacaat caaggccgca atgacgtata
    14581 agggccatct aatctttttt ttcatttctt atttcctcca aaatactctc gggaacatag
    14641 caggcaatat cctgaccaaa cttcaaaagc tctctaacgc ctgatgaact gatatagaga
    14701 tgttcaggtc gactatgtaa ataaatagtc tctatatcag aagacagctg atgattgtag
    14761 taatcaaaac tggcttcata ttgcaaatcc gacgcattcc tcaaaccacg cactagacaa
    14821 gtagcaccca atctttttgc aacatcgacc accaattcat catgagaagc cacgacttca
    14881 acattttcca gatgtcccaa agccttttct agcccccgtt tacgattttc gataggaaga
    14941 aatccttgtt tgtggggatt aaaaaaaata ccgacataga gcttatcaaa gagtctgctc
    15001 gcccgttcaa tgatatccag atgcccattt gtcatcggat caaatgagcc tgtgaataag
    15061 ccaatcttat ctgacataga ctgtcacctt actaattcca taaatctttt ccttccagat
    15121 acccagacag gcaatttctt ctggaagttc aacggcctta tccgtctcgc acacaaccat
    15181 aacatcttca gaaaaaagct ctctctcagc cattttttca atatctgcta cgatttgttc
    15241 cttggtatag ggagggtcta agaaaacgag gtcaaattcc ccagatacct gttccaatgc
    15301 cctttctgca tccatcttga ggagttgaaa ttttccaatt tccttggtta tctggatatt
    15361 ttcagccacg atggtctgag ccttacggtc tcgctccacc aaaacagcac tggacatgcc
    15421 acgcgatact gcttcgatag ataaaccacc actacctgca taaaggtcca agactcgtcc
    15481 cccttcaaag tagggaccaa tcatgttaaa aatggctccc ctaaccttat ccgaagtagg
    15541 tcttgtcgtc ttgccttcta gtgtcttgag gggacgtccc ccatagattc ctgatacgat
    15601 tttcatactg tttattatac caaattatag acaaaaagag aaagaaaacc gaaccttgcg
    15661 gttcgattct ctacaaaata ttttcgtaag tatcgcggac ttcttgaggc caaacacttg
    15721 tttgcacttc tccgatgtgt ctcttgcgaa gtaggaacat ggccatacga gattgtccaa
    15781 ttcctccacc gattgtcaat gggaatagac cattcaacaa agacttgtgc cattccaatt
    15841 ccaagcggtc ttcatcacct gtaatttcca cctgacgtct aagagtttct tcatctacac
    15901 gaattcccat agaagacaac tcaaaggctc cacctaaaga ctcattccag acaagaatat
    15961 caccatttag acccttgtag ccattctcag actcgcttgt ccagtcatca tagtctggtg
    16021 cacgtccatc gtgcggttta ccatctggca actcgccacc gataccaatc aaaaagacgg
    16081 ctccaaattc tttacaaatc gcattttcac gttctttcgg tgtcaagtct gggtagcgtt
    16141 ctaccaattc ttctgtatgg ataaaggtga tttgttttgg caagatagac tcgatgtcat
    16201 agcgggcttc aacagctagc tcagtcaggc gaatagcctt ataaatcttc tcaactgttt
    16261 cttttagata aacgatgtta cgcttaccat ttgggataac cttctcccag tcccactggt
    16321 caacataaac agagtgggtt gcatccaagg aatcctcatc tggacgaagg gctttcatgt
    16381 ggacaaagag accctctcct tcaccaaagc caaaacgagc caaggtgtgg cgtttccatt
    16441 tagcaagtga gtgcaccact tcataagtag catcagggat ttggagaacc ttgaccgata
    16501 ctggattttc cacaccagac aggttgtcct gcattccgtc accgacctta ctcaagatag
    16561 gaccttgaac ttcgacaact tctagcttat ctttcaaata ctgggtaaaa gtgtttttga
    16621 caaaggaaat ttcttcttgt tgatggataa aacttttctt catagacaac tcctcaaaaa
    16681 tttaataaac gtattataca cctatttcca agaaaagaaa agagaaaatt taaaaaaatc
    16741 agtgtcactc aaaacactga tttcatagac cttttagtca ttgcgaccaa agatacgtag
    16801 aatacttagg aaaagattga taaaatcaag atagatgctg agagccattg acaccaccca
    16861 acctgtcgct acacgacctt gtgattgttc ataggcgagg cgaatctttt ggttatccca
    16921 agcaatcaac cctgaaaaaa ccaaaaccat ggctacgcta atcatataat caaagaagcc
    16981 actagccaag aaaatattga ctaccatagc aatcagtaaa ccaatgagag ctgccatcat
    17041 tgctcgacca atcccactca agtctttctt ggtaaagata ccaactgccg ccatgacaaa
    17101 gaagagaagg gcgctcgata caaaggcaga taaaactgta cccggagtat agaaagccac
    17161 cacaaaacta agggtaaagc cgtttaaaac ggagtaaagt aaaaatactg gaagagcagc
    17221 tggactattt cttgaagcca tgctactggc aacgaaaacc aaagccaact ccgcaaaagt
    17281 agcaatggtc aaccagagac gcccctgcat caaaaagtaa accaactgag actgaaagac
    17341 cgtcaacata aggcctgata ccaaagcaga tagtccgatt cccagaccaa caaaggcata
    17401 aaccttagcg taaaattgat tgagacctgc acggtcatgt ataatagtgt gattcatcca
    17461 tttctccttt tctgtgaaca tctttccttg attataacaa aaaaagttaa aattagctta
    17521 aatggaagat taaaataccg gctgcaaccg caacattcaa actctcggcc tgccccttca
    17581 tgctaatatg gaccaactgg tctgcacttt cagccataag gggactaatt ccttgaccct
    17641 catttcccat aactagtaca aaattttcta tagaaggcag ttctctgtaa tcaacagaat
    17701 ctttagatag ggttgttgct agcactggga tagctacctt tttagtttcg tcaagaagcg
    17761 cttgactagt catccggtaa atgggcagat ggaaatgact accttgcatg gaacgtaggg
    17821 ttttgagact gtagatgtct gccgacttat ctgaaacaat cactcctgta aaacctgctg
    17881 catccgcagt tcgaatgata gttcctacat taccaggatc ttgcacatct tccaaaaaca
    17941 agaacttgcc ctgactcaaa tcagcttgtc ctacttcttc tctttgaacc acggcaacaa
    18001 ttccctgtgg agtctgagaa tccgccaaat ctagcaaaat atcctctgac acccagacag
    18061 tttgcgaaaa agtagctaac tgatctcgat aattttctag ggtaaagatt ttctcaatcg
    18121 tcactccagc ttgaacagct tcttcaaaca agtgccagcc ttcaatcaaa taggctgact
    18181 tgcggtattt tttttggtgt aatttcttgg tattttttac cacagaattg gcttttgagg
    18241 ttataatagt catagaaata ttataacaca atcaaagggg tttggtatgc aaaaggttag
    18301 aatgattgcc caaggtaggg tacagggagt cggctttcgt tggggtgttt acagcttggc
    18361 ccttgaaatc ggtggcatca caggtcgggt atggaataac gacgatggca cagtggaaat
    18421 cttagcccaa gcagactcat ctgctatcat ggcaaaattt atccaagaaa tccgaaaagg
    18481 accgacacct ttttcaaaag taagctactt agatgtcaaa ctaagcaact ttcctcccta
    18541 ctctgacttt aaaatcgcaa attaggtctc cagaactatt gtatattttg taaaaaaaca
    18601 gtagaataga aaggtataat ttttaaagaa ggaataaaaa cagtgaaatc tattaaacgt
    18661 tttgcattct cggctatggg agtggctatg ttgcttgtct tgactggctg tgtcaatgtc
    18721 gataaaacca caggtcagcc aacaggattt atttggaata cgatcggagc gcctatggct
    18781 gaagccatca agtacttcgc tactgataaa ggtctaggct ttggtgtcgc tatcattatc
    18841 gtaaccatta tcgtacgctt gattatctta ccacttggta tctaccaatc atggaaggca
    18901 acgcttcact ctgaaaagat gaacgctctc aagcatgtcc ttgagccaca ccaaacacgt
    18961 ctcaaagaag caactactca agaagaaaaa ctcgaagccc aacaagccct ctttgccgct
    19021 caaaaagagc acggcatcag catgtttggt ggtgtaggat gtttccctat cctccttcaa
    19081 atgcctttct tctctgctat ctactttgct gcccaacata ctgaaggggt tgctcaagca
    19141 agctacctag gcattcctct aggttctcca agtatgattt tggttgcctg tgctggtgtc
    19201 ctttactatc ttcaatcgct cctttcactt cacggggtag aagatgaaat gcaaagagaa
    19261 caaatcaaga aaatgattta catgagccca ctcatgatcg tcgtcttctc cctcttctca
    19321 ccagccagtg ttacacttta ctgggttgtc ggtggtttca tgatgattct ccaacagttt
    19381 atcgtcaact atatcgttcg tccaaaactt cgcaaaaaag tccgtgaaga actagccaag
    19441 aacccaccaa aagcaagtgc tttctctaaa ccaagtggac gaaaagacgt tacccctgaa
    19501 caaccaactg ctatcacaag caagaaaaaa cacaaaaatc gcaacgctgg aaaacaacgt
    19561 tcgagataag aagaaaaagg cttagctgat ttgctaagtc tttttgcaaa tcaaaagccc
    19621 gatgtttcca tcaggcttct tttctatcca tgtacacgtt tcatataatc ttgataactt
    19681 tcggtatcca tgagtttttt agcgttcttg acgcgatctg ctgttggtgg tttgactcct
    19741 tcgagggaat atggaattcc aagttcacgc cacttgaact cacccatggt gtgataaggt
    19801 agaatttcaa acttatcaac atttttgagg gtcttgacga acttaccaag ttcaatcaag
    19861 tcatcatctc tgtctgtcaa tcctggaact agcacgtggc gaatccagac aggttttcca
    19921 atatctgata gatactgggc acaagccaag atatttttat tagtttgtct agtgacaatc
    19981 ttgtgctgtt cttcgttgat ttccttgata tccaaaagaa ccaagtcagt gacagccatg
    20041 agtttgtcaa acttctcaag gtaacgtggt ttattacgga aaggaagagc acaggtatcc
    20101 aaggtacagt ggattccttg ttccttagcc ttggtgaaga gagcaatcag gaaatcaatc
    20161 tgcaagagag cttctcctcc actgactgta atcccaccct tatttcccca gaaaccacgg
    20221 tagcgcaagg cctctgtcaa gacatcatct accgtccgtt cacgtgactt attggactcc
    20281 atagcccaag tgtctgggtt gtggcaatac tggcaacgca tgtgacagcc ctgcaaaaag
    20341 acaataaagc gaataccagg cccatctact gacccaaagc tttctgtcga atgcaccatt
    20401 cctgtcactt gtccataatc aattgtttct tcagacatat cgttaccttc cttgaaaacg
    20461 ttttatagtt ttattatatc atgacttgaa ggaaaaacaa gatttttgcc tattttttag
    20521 aaagcaatct gtttttcaat ttcattttca atctattatc agattattct tcaaaatcga
    20581 agccatataa ggttgcaaaa tagtcctcag ggcgctctgc acggcggatt tgacgggctt
    20641 taccttcttc ggtatagagg atttccgcag aacgtaattt ggcattatac tggtagccca
    20701 ttgaaaatcc gtgggcacct gtatcatgaa tgaccagcaa atcaccgatt tctgtatgag
    20761 gcagttcgcg attgactgca aatttatcat tgttttcaca gagtgaacca accacatcta
    20821 ccgcttcagc tggtccatct ggatgggtca cgttgctaat atgatggtaa gctccgtaca
    20881 tagctggacg catgaggttg actgctgagg catccacacc tagataggta cggtaggttt
    20941 ccttcttatg agtcactctt gtgactagag caccgtgagg tgccagcata aaacgaccca
    21001 attcggtgaa aatcttgacc tgaccaagac ctgctgacgt aagaacttct tcatacacct
    21061 tacgaactcc ctcaccaatc aaggcgatat cgttcggctc ctggtctgga cgataattaa
    21121 caccaatacc gccagaaaga ttgataaagt ctagcgaaat gcccaacttt tccttgattt
    21181 caacagccag ttcaaagagc tgacgagcca actctggata atagagatgg gtcacggtat
    21241 tggacgctag gaaggagtga atcccaaaag tcttggctcc tttttccttc aagatagcaa
    21301 aggcttcaaa gagctggtcc ttggtcatgc caaacttagc ctccccagga ttgtccataa
    21361 tgtctgtccc cagttcaaaa acgcctccag gattataacg acaagagatg atttctggaa
    21421 tgcctgctac tctctccaga tgttcaatat cttcaaaggc atccaagtta atggtcgcac
    21481 ccaattcacg tgcataggcg tattccttgt ctggcgtgtt gttggaagag aacataatct
    21541 cagaacccag aaagtccagt ttatggctca tcaaaagctc tacataacta gagcagtcca
    21601 caccacaacc ttcttcttgg agaattttca aaatagctgg agttggagta gccttaactg
    21661 caaaatattc cttaaagccc ttgttccacg aaaaagcttg gttgacggct cttgccttct
    21721 cacgaatccc cttctcatca tacaagtgaa agggagtcgg gaactcggca acaatcgctt
    21781 ctaactcttc tctattgata aatggtgttt tcataagctt ccttctattc tgtttgcaaa
    21841 gaaaggctgg gacaatcgtt ccaaccttta gttctatctc ttatttatct tcgtcaaaga
    21901 tattgccaac ctcaacatcg atggcttggt tcttgacatc aatattagtt accttcaaga
    21961 gtgacttgta gtcaaactgc ttttcacgtt cttcttgggc attgtccgca aagaccgcta
    22021 cacctgccag ttctgagtcg aactcgcgca agagactaat cataccgttg accgttccgc
    22081 cacctttcaa gaagtcatcc acaatcaaga cacggctgcc tgccttaaga ctacgttttg
    22141 aaaggaacat tttctcgata cggtcaccac ttgaacctga aacatagttg acgctaacag
    22201 ttgaaccttc ggtaattttc aggtcacggc gcacaatgac aaaagagaca ttgaggacat
    22261 tggcaactgc atttgcaagt ggcacaccct tagttgctac ggtcataacc gcgtcaattt
    22321 tttggtccat aaagcttttg gcaataatac gaccaatatt tttcaagatg gctggtgtgc
    22381 taagcaaatc agacagatag atataaccac ctggcaagat acggtcactt tctgacaact
    22441 tggtacgcaa gtcttcaacc atttccttag catcctgact cgaaatagac ggtgtgaaga
    22501 tgacacctcc gccagcccca gtcactgtct ggatatgacc gatttcaatt tcctcaaagg
    22561 cgcgtttgat aatgacgata tcttctgaga tggatgattt agcagactca tacttttcag
    22621 caaaagtatt gagactagtt agtttataag gattattaat caaatagttg gaaatgacaa
    22681 ccatccgatc acttcttctt aatttcattt ctatttccta ttccattttt attcaaaaaa
    22741 tcaaaaagca aactagaaag ctggtcgctg gtggttcaaa acactgtttt gagattgtcg
    22801 atagaactga caaaccctgt aatatacctg catatataca tacgacaaag cgatactacc
    22861 ctagtttgaa gatattttcg aagagtattc atttttgtct tttacttatt ataccatatt
    22921 cacataaaaa aacgaacatt cttatcctaa aaaatgctca tttttcttaa attatcaatc
    22981 taaatctggt ttatagaagg aacgattatc catagcgaag attttattgg tcatctctcc
    23041 tttatccacc aaagccagag ctgttgacat catcatcatg cttgcatcca gattgtcaat
    23101 catatggata atctctgcct ccataatacg tggacggact ggacttccat attcaagcaa
    23161 gccgtggtga ctgaggatga catgacgaag caaaacgact tcttccttgg tatcatcgat
    23221 gccgagttcc ataactgtct tggtaatttc gctatcaatg agagcgatat gtccaagaag
    23281 attacctcgc actgtgtact ctgtctggtc tggccccgtc aactcgatga ccttagccaa
    23341 gtcgtgcaac ataatccccg catagagcag actcttattg agctgaggat aaacttcgct
    23401 aatagcgtct gccaaacgta ccatggtcgc cgtatgataa gccaaccccg tttcaaaggc
    23461 atggtggttg gtcttggcag ctggatagga gtagaattcc ttatcatact tggtgtagag
    23521 atttcggaca atccgttgcc agacaggatt ttcaattttg aaaatcattt gcgacatgta
    23581 gtcacgaatt tccttgacat caactggtga cttgaccttg aaatcggctg ggtcattggg
    23641 ttcaccagct tgaggcaggc ggagagtaat ttgattgact tgaggggtat tgttataaac
    23701 ttctcggcgt cctttcatgt ggacaacctt acctgcggta aaggcctcaa tgttatgagg
    23761 ttgggcatcc cagagcttcc catcaatctc gccactatca tcttggaagg taaaggctag
    23821 gtagtttttc ccagctcgag tttgcctcag gtcagctgat ttgattaggt aaaagccttc
    23881 aaatagctca tcttttttca tgtgactaat cttcatattc ttcctcattt tcttgaaaat
    23941 ggagtagatc aagcgcaggc tcgccttctg acaactcaat gtgacggagc gtccgctcga
    24001 tagctatggt acgacggttt aataattcat caatattgcc agaggcatgt tggagatgtt
    24061 tttgtgcctt gaccagaata ccaccaaact tgccaaactc ggtcttgaca ctggcaagag
    24121 tcttgctgat atggtcggca ctcttttgga tattaagggt cttgaaacca actgataggg
    24181 agttaagaag ggctgatagg gtacttggtc ctgcaacaat aatctgttct tcccgtctca
    24241 aatcatcaaa gaagaccgga ttgcggacga tttctgagta gagaccttct gtcggaacaa
    24301 acaaaactcc aaaattggtc gtccgaggtg gtgctatgta cttgttccta atatccctag
    24361 caaaacgctt gacacttgct aggagtgact tacgacagcg ttcgatttca tccttgtcac
    24421 ctacttcata ggcttcttct aagcggtaat aatctgccag tggaaactta gagtcaattg
    24481 gtaggtagac gtattcttgg tcgccttgtc cgggtaactt gatggcatac tccactcgtt
    24541 cactagagtt ttcaaccgtt gcgtattctc gttcgtactg ggcaggtgtc atgatgtctt
    24601 caataatttg ccccagttgc aattctccca gaatccctcg cgtcttggtt ccagagagaa
    24661 ccttgttaag agctccgaca tcacgggcaa ctgtctgcat ttctccaaga ccacgattga
    24721 cagactccag ttgtttagaa actgtctcaa aggaagcctg taagcgtgtc tgcaaggtct
    24781 tttctagttt ttcctcgacc gtctggcgca tttgttccaa acgttgctca tttgattcct
    24841 gcaaggcttg gagacgttgg tctgtcttgt ctctagtttg gaggagatta tctgtcattt
    24901 cttgacggac ttgggtcagg ccttggtgca attcaatccg cacttcttgc aaacggtcgc
    24961 tgacagccac ttccaaatct ttttggtcta actggctggc ttgtctggct tggtcaaagc
    25021 ggtaatccaa ctggtctgac aaatgatctg cctgatcctc caagctctta cttaagtgtt
    25081 tctcctgcct atcctgtctt tgccaaatca gaaagagacc agctaggttg gcaattaata
    25141 atagaataag taaactttcc atcctacctc ctgtccttgc tatgcagtac gaccacataa
    25201 ccatctgggc aagtcaccga cacttcccta tctatatatt cgttagaagc gtacactttt
    25261 ttaaagaaaa aattctcctc tgtcaactca tacttagctc caagaatagt cagctggcta
    25321 tcccgaactg gcataaaggc tagatagtcg tagtctgaac gaggttctag ctgactggtc
    25381 ccttctgaac aataggcaat caaattttgc ccatcctcaa tttctatttg acgcatatag
    25441 ggtgccaact taggattgct aggtagaaag acattggcca acatatggtc aatacggcca
    25501 cccaaggcac cgaaaatagt gacctcagcc tgaggatttt gttcaaagat ggttaagaga
    25561 gccaattcca gatctgtatc atccttttct ggtcgggctt ggacaaaatg ctgggcacgt
    25621 ttttgaatca cctgtcgctc ttcttccgtc acagaatcaa aatccccaac tgctagagca
    25681 agaagcaagt cttcttccaa gacccagagc gagcctcgat ccaccccaac aaaagcatca
    25741 aaatctgtcc gataatgacc gcggtctccg cctgcaaaaa cggcaaccct agtccagttg
    25801 ttttctgaga gtttgtactc gctcattgac atctccctta aagacatagg aacctgctac
    25861 aaaaacggtc gcaccagctt ctttagcttg ggcaatagtt tggtcatcaa tcccaccatc
    25921 cacttcgatt tcaaagttca aacctttttc ctcacgaaga gcaaccaact cacgaacctt
    25981 atccatggtt tctggcagaa aggcttgccc accaaaacct ggattaaccg tcatgactaa
    26041 aacttggtca actagatgaa ggacgtgctt gatggcttca actgatgtgc caggattgat
    26101 aacgactgaa ggcttaactc cgagtgaacg aattttttgg agggcgccat gaatatgagg
    26161 cgttgcttct acatggatac tgatgatgtc tgcacctgca cgcgcaaaat cttccagatg
    26221 atgctcaggg tttgacacca tcaagtggca atcgaaaacc atcttactat gaggacgaag
    26281 gctctcgacc acacctgcac caaaactgat ttgcggtaca aaatgactgt ccatgatatc
    26341 gatatgggca tattctgccc cagttgcttc tagacgtttg atttcacgtt caaagttggc
    26401 ataatctgct gccagaattg acggagcaat cttgtattga gacataggtt tctccttatt
    26461 ttggaatttt tttgctgact tttttatagg tttctctacg attttcaatt tcactaagga
    26521 attgcaggta attgtcaaaa cggaaggttg caataacacc ctcttcaaca gctggtttga
    26581 cggcacaaga cggctcatgg gtatgggtac aagtacggaa cttacaatct cggctaacag
    26641 tagcaatctc tgggaaagcc tgattgaggt cttcagccct tgatacttca tagtccaagg
    26701 atgaaaatcc tggtgtatct gcgattttac ccccgttgag attgtaaaaa ctaacagctc
    26761 gagtggtatg gcgaccgcga cctagactgt ctgaaatttc tcccgtttca agattgaggt
    26821 ctggtgcgat tttattgaga agagttgact tcccaacacc tgtctgcccc ataaagaccg
    26881 taaccttgcc tgttaacaaa gacaggagtt cctctttact ggtcacaaag tcatagccga
    26941 tgtcaccata ggtctgctgg taaaaatcca gttctcccct atcttccaac aaatccattt
    27001 tggaaatata gacaatggga tggatgccct tgtgctccaa aagaaccaag aaacgatcca
    27061 gcaaattgct gttaaaatca ggttccttga cggacatgat tactacagct tgatcgatat
    27121 tgacaatagg cggacgaacc agactgtttt tccgttcgtg aattttgagg atatagcctt
    27181 ctgaattttc ctcggcagag aaatctaccc agtccccaac ataaggggta tggccttttt
    27241 tacggaaatt cccacgcgcg cgtgtttgat aaacctggcc atcactctcc acatagtaga
    27301 aacctgccaa ggctttaatg atttgtccct gcatcttaga gtccttgccc tttaagtgcg
    27361 tctgctagac cggcaaattc tgccaagctg agagcttccc cacgcacact tggtgacaag
    27421 cctgcctggt ccaaagcctt ggtcagcttg tccttgacct cttcagtctt accaaagtaa
    27481 cctgtcaagt tattccacaa ggtcttgcgg cgatgggtaa aactagcctt ggaaacctta
    27541 aagaaaaagt tctcatcttc tactgctacg gctggctctg gacgacgcac cattttcaag
    27601 atggctgaat ccacatttgg cgctggcaca aagaccgtac gaggcacgat aaaggcaacc
    27661 ttggctgtca tgtaatactg cacggcgata gacaagctac cgtaagcctt ggtgttaggc
    27721 tgggctgaaa tgcggtccgc tacttctttc tgcatcatga ccacaaactc actaaaagga
    27781 atgccactct caatcaagtg catgagaata ggcgtcgtga tgtagtaagg caaattagcc
    27841 actaccttga ttggcaggtt aggattttta aaattttgga tatgttgcgc caaatcaacc
    27901 ttgagaatat cttcgttaac tacagtcaca ttatcaaaat cacgcagggt atctaccaaa
    27961 attggcacca aacggtggtc aatctcaaaa gccatgactt gggctgcacg ctcagccaga
    28021 aactccgtca aggcaccaat acctggcccg atttcgatga cattgacctg gtcatcaatt
    28081 tcagccgtat ccacaatttt ttgaaggata ttggtatccg tcaggaaatt ttgcccgaag
    28141 gactttttaa aggtaaaacc gtgacgctcc agcactgcct tggtcacgct ataatctgca
    28201 attctcatct attctctttt ctattatctg ttactactat tgtaacacat tccccttgta
    28261 ttttgggaat gaaaatcata ccaaaaaact atctttcctc atgaagaaag atagtcttgg
    28321 aaatctttaa aagtggcctt aacagttctc tcgaaacatg cgttcatact cttcgaaaat
    28381 ctcttcaaac cacgtcagct tcaccttgtc gtactcaagt acagcctgcg gctagcttcc
    28441 tagtttgctc tttgattttc attgagtatc aaaaaaatca gcttcttgat aacttctatt
    28501 ttcgggtagg gtcaaatcca catcctcctc cacatcatcc actgtcagtt caaccgtttg
    28561 aggtaaattt ttcaaacgat aatgcttttg cctttaccct tcaatactag caaaatcaag
    28621 caaatagcca tgcactagga tttgaaccaa aaggagatga ttggtcatat ttttatcttc
    28681 taaaccacct ctggcactag gataatcaga taccctgcga atacggattt tttcacacat
    28741 gagcaagttt cctcatatca atgatttcat cagccttatt ccagactagg ggattatggg
    28801 tcgcaatgat gataattcga ttttcatctt tcaaatccac caagagattc ataacctcct
    28861 ctgaattttc aggatcaaga gctgctgttg gttcatctgc caaaatcaag ggtggatttt
    28921 tcaaaatagt ttttgcaagg gcaactcgtt gggcctctcc cccagataaa gtatagattt
    28981 tttgttctaa atccaaatac cctagattaa ctttttctaa agcccccact tgcctttcca
    29041 aacgttctac ttttgagatt ttctgaccaa caaaacccaa atccaaattt tctttgattg
    29101 attggttttc taagaggccg aaattttgaa agagatagcc catctggtct cgaaaatact
    29161 cacgagtagg aatggttttt aaatctttcc cctgatagag aacccttcca ccatctatct
    29221 tttctagctt tcccaagata ttcagcagcg tcgtctttcc gcttccactc tttccgatta
    29281 aggcataaac cttgcccttc tccagcttga gattcaaacc agagaaaatc gcgcggtcat
    29341 taaatttctt ttccaatcct tgaatatcaa tcatcttatt ttcctttcaa tactgccatg
    29401 ctaccagctt cttctttttt gtcctgcctt actaaaatca agagggcgtt aagcgtaaac
    29461 aaagctacaa ctagagcgct aatcaaacca tctcttgtca aaatactaga taggactagg
    29521 ccaagcaaga gaactcctcc ttgcgccagt aaatacttgc catgaagttc atagattgtc
    29581 ataccagcaa gacgtttaat cataagctcc cgtctgaact gctcaaaata gagaagattc
    29641 atggaatcaa ataacaagat agccgtagac agggtcaaaa tcgtcccaat aaggagagag
    29701 taaaactcca cccgtttgtc atttagtacc ttggcaaaaa atagctggct ttttaccaag
    29761 taagaaacct tgtgatacag accttgctct tttagagctg tgatgctgtc tccatatcga
    29821 tctagtttca aggcactatt agccgtagtt ccccacaaca tccctgcaac aggtcttgtt
    29881 ccagtcgctt gcggcgttag aactacaata atcggatctt ttaggtactg gcgtgttgtc
    29941 ttgtacccat cattatagag gaaacgttcc tgtcctgttt ctgtaaaagc tagccttacc
    30001 tgtgggaggt agtgtttctg actcgtcact tctacacttt cagatgaaaa gttttgcagg
    30061 taatctgtaa acaatccttg gtagtagaca gactgctctc gcaagctctc aggcaagatc
    30121 agcccaaact ctccctcaga caagttttgc atcttgtcca taaactctga agaaacggta
    30181 atcttttctt ctatcagata gcgtggtgag acatagataa cattccctga cggtgtgtag
    30241 tcactgagac gattgccatc taggtccact tctgctccat ctgagaaatt gtcaacattg
    30301 ctcataatat aaaaagagtc tgtattggct aaccgttctt cagtaaatgt ctgccactca
    30361 cgattatcct tacgcgttcc ttcttcatcg gcaaatgcac tagaccaacc aaaggataga
    30421 cggtaacggt ctgaggactg gctccattta ttgctagctc tctccatttc ctgcatttca
    30481 cggtagtggg gtaggagagc tgtcgcactc gatccgacca ccaatacagc taagagttgc
    30541 cccaccatca tcaatgtcat catacgtttg agagggagtt tccctttcaa tagatccacc
    30601 agactatttt cctgtaaacc aagtagatag acgacactta gtaaggtaga aatccctgcc
    30661 aaggtcaatc cataaagtag aagagcaatg atgaccagtt gcaccgttgc cataaacaag
    30721 gcaccttgat accagagaat ccccaatccc aaaagactgg ataccagcac tgagcagata
    30781 agctgtctca catcttctaa cactggtctg agagcaactc caaacaagct ctcaccagct
    30841 attaagcgaa tccctgcctg acgaagggat ttgatccgat aaatcagggt cagactcata
    30901 aaggtcagca gaaaaatagc taaactcagt agcaccatag gggtggccgt caatagtaag
    30961 actatcgaaa atggatcttc tccattcgaa acaaagcctt gataaccaag ctctttcaag
    31021 gtggtctgta agctcactcc atccaaactt cctgacacaa tcaagtaaga ccctaataaa
    31081 tcactcgttt ctgcactctc cttggaggaa atggtcagac cttctggaag ctttccctca
    31141 ccataagtgg cataggtaaa gcgtgtttct ccagcttcat ttggctcaac gattcgacga
    31201 gcaatgagac tattgtgctc taccgccaag tgactcaaag cctctgtcac ctcttcccga
    31261 ctagctactg gttgatttat catcattcct gtcacatcaa cagaaggata gttaccaaat
    31321 aaaatctctt ctcgatggtc aacggaagtt atcaccatat aaagcgatac caataccatt
    31381 gaaatcaaaa taaataaacg tttcatcatt tcctccaaaa agttacttgg tcacttagtg
    31441 agaaggtgac caagtaaagc taaacaatag ggctatacca ttctaaacat tatagtagaa
    31501 tgcaactttc tctccccaag aagtacgaat ccatgcacga gaagtccctc cagcaccagc
    31561 atatcccttg tcagatgaac cagtttttct gctaacaact gttgaactat ggtattttga
    31621 agggtgatag tagtttgagt aagcaccacc gttacctgaa ccataagtcc aaacaccacc
    31681 ttctgggtat tgaacggctg cagaggcaac tgctacactt ccacctaatg ttgctgcaat
    31741 gctcgctaca agagctaatt ttttaactgt ttgtttcata ttgatactcc tctatttttg
    31801 attacaagat tgttgagtta cttgctcctc tttccattat caacgttgat actattatat
    31861 taccaaatga tattctaaaa ttatttgttt ttctctaaat tgtcaaaata gcaccctgaa
    31921 atgaactttt atagtcatta tact
//
LOCUS       DAVLXF010000012        31919 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLXF010000012 DAVLXF010000000
VERSION     DAVLXF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.012"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            52..1041
                     /gene="manX"
                     /locus_tag="VYO12_000519"
     CDS             52..1041
                     /gene="manX"
                     /locus_tag="VYO12_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1587171.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQAAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1069..1872
                     /locus_tag="VYO12_000520"
     CDS             1069..1872
                     /locus_tag="VYO12_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1587172.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1896..2807
                     /locus_tag="VYO12_000521"
     CDS             1896..2807
                     /locus_tag="VYO12_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1587173.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2863..4197)
                     /gene="pepC"
                     /locus_tag="VYO12_000522"
     CDS             complement(2863..4197)
                     /gene="pepC"
                     /locus_tag="VYO12_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1587174.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(4414..5136)
                     /locus_tag="VYO12_000523"
     CDS             complement(4414..5136)
                     /locus_tag="VYO12_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1587175.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DINTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     KPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(5449..5679)
                     /locus_tag="VYO12_000524"
     CDS             complement(5449..5679)
                     /locus_tag="VYO12_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1587176.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            complement(5689..6930)
                     /locus_tag="VYO12_000525"
     CDS             complement(5689..6930)
                     /locus_tag="VYO12_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1587177.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDLLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVIFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSLVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(6952..7104)
                     /locus_tag="VYO12_000526"
     CDS             complement(6952..7104)
                     /locus_tag="VYO12_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587178.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(7125..7403)
                     /locus_tag="VYO12_000527"
     CDS             complement(7125..7403)
                     /locus_tag="VYO12_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1587179.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(7396..7659)
                     /locus_tag="VYO12_000528"
     CDS             complement(7396..7659)
                     /locus_tag="VYO12_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1587180.1"
                     /translation="MSKMSMSIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(7761..12152)
                     /locus_tag="VYO12_000529"
     CDS             complement(7761..12152)
                     /locus_tag="VYO12_000529"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1587181.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VDKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     GAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEAIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(12256..14337)
                     /gene="fusA"
                     /locus_tag="VYO12_000530"
     CDS             complement(12256..14337)
                     /gene="fusA"
                     /locus_tag="VYO12_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1587182.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(14762..15232)
                     /gene="rpsG"
                     /locus_tag="VYO12_000531"
     CDS             complement(14762..15232)
                     /gene="rpsG"
                     /locus_tag="VYO12_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1587183.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(15252..15665)
                     /gene="rpsL"
                     /locus_tag="VYO12_000532"
     CDS             complement(15252..15665)
                     /gene="rpsL"
                     /locus_tag="VYO12_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1587184.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16082..19879
                     /gene="spuA"
                     /locus_tag="VYO12_000533"
     CDS             16082..19879
                     /gene="spuA"
                     /locus_tag="VYO12_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1587185.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWHLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDLAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDRQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            complement(19979..21028)
                     /locus_tag="VYO12_000534"
     CDS             complement(19979..21028)
                     /locus_tag="VYO12_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1587186.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SDGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHIPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            21085..21222
                     /locus_tag="VYO12_000535"
     CDS             21085..21222
                     /locus_tag="VYO12_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587187.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTNEVSNHTYGKATLTWFEEI
                     F"
     gene            complement(21299..23107)
                     /gene="glmS"
                     /locus_tag="VYO12_000536"
     CDS             complement(21299..23107)
                     /gene="glmS"
                     /locus_tag="VYO12_000536"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1587188.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYFEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(23300..24679)
                     /gene="bglA"
                     /locus_tag="VYO12_000537"
     CDS             complement(23300..24679)
                     /gene="bglA"
                     /locus_tag="VYO12_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1587189.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(24779..26632)
                     /locus_tag="VYO12_000538"
     CDS             complement(24779..26632)
                     /locus_tag="VYO12_000538"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1587190.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(26645..27904)
                     /gene="rseP"
                     /locus_tag="VYO12_000539"
     CDS             complement(26645..27904)
                     /gene="rseP"
                     /locus_tag="VYO12_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1587191.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTVLPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGGKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(27926..28729)
                     /locus_tag="VYO12_000540"
     CDS             complement(27926..28729)
                     /locus_tag="VYO12_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1587192.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(28738..29496)
                     /locus_tag="VYO12_000541"
     CDS             complement(28738..29496)
                     /locus_tag="VYO12_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1587193.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(29727..30191)
                     /locus_tag="VYO12_000542"
     CDS             complement(29727..30191)
                     /locus_tag="VYO12_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1587194.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(30280..31251)
                     /gene="ruvB"
                     /locus_tag="VYO12_000543"
     CDS             complement(30280..31251)
                     /gene="ruvB"
                     /locus_tag="VYO12_000543"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1587195.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            31412..31504
                     /locus_tag="VYO12_000544"
     CDS             31412..31504
                     /locus_tag="VYO12_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1587196.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         9199 a         7476 c         6178 g         9066 t
ORIGIN      
        1 cttaaagttt tcttaaatga aaactaaaac aaaaaaggag gaatgacaat aatgagtatc
       61 ggaatcatta ttgcgagcca cggcgaattt gctgcgggta ttcatcagtc aggatctatg
      121 atctttggtg aacaagaaaa ggttcaagtt gtgaccttta tgccaaatga aggtcctgat
      181 gatctatacg ctaagtttaa taacgctgtt gctgcatttg acgcagaaga tgaggttcta
      241 gttttggctg acctttggag tggttctcca tttaaccaag ctagtcgcgt gatgggagaa
      301 aatcctgagc gtaagtttgc catcatcaca ggacttaact taccgatgtt gattcaagcc
      361 tacacagagc gcctcatgga cgctgctgca ggtgtagaaa aagtcgctgc taatatcatt
      421 aaagaagcca aggatggcat caaagctctt ccagaagagc taaatccagt tgaagaagtt
      481 gcaagcgctg cagctgctcc agttgcccaa gctgctatcc cagaaggaac tgttatcgga
      541 gacggtaaat tgaaaatcaa tcttgcccgt cttgacacac gtctacttca cggtcaggtt
      601 gcaactgctt ggactccaga ttcaaaagca aatcgtatca tcgttgcttc agataacgtg
      661 gctaaagacg accttcgtaa agaattgatt aaacaagcag ctccaggtaa tgtcaaggct
      721 aacgtggttc caattcaaaa actgattgag atttcaaaag acccacgttt tggagaaaca
      781 catgccctta tcttgtttga aacacctcaa gatgcccttc gtgccatcga aggcggcgtg
      841 ccaatcaaga ctcttaacgt tggttctatg gctcactcaa caggtaaaac attggtcaat
      901 accgttttgt ctatggacaa agaagatgtt gcgacatttg aaaaaatgcg tgacttgggt
      961 gttgaatttg atgtccgtaa agtaccaaat gattctaaaa aagatttgtt tgacttgatt
     1021 aacaaagcca atgtcaaata agccattatt tatgaaagga ttttaaacat gtctattatt
     1081 tctatggttt tagtagtcgt tgtagccttc tttgcaggtc ttgaaggcat cctcgaccag
     1141 ttccaatttc accaaccact tgtagcctgt acccttattg ggcttgtaac aggtcacttg
     1201 gaagcaggga ttatcctcgg tggatcgctt caaatgattg cccttggttg gtcaaatatc
     1261 ggtgctgcta tcgctcctga tgctgcactt gcttctgtcg ctgctgccat tatcatggtt
     1321 cttggtggtg actttaccaa gactggtatc ggtgttgccc aagcggttgc tatccctctt
     1381 gccgtagctg gacttttctt gacaatgatt gttcgtacaa tttcagttgg tttggttcat
     1441 actgcagatg ctgccgctaa aaaaggtgac ttcggcgctg tggagcgtgc gcatttcatt
     1501 gcgctactct tccaaggact tcgtatcgcg cttcctgcag ctcttctcct tatggtacca
     1561 actgaaactg tacaaagtat ccttagtgcc atgccagact ggctcaaaga tggtatggct
     1621 atcggtggtg gtatggtcgt tgccgttggt tacgccatgg ttatcaacat gatggcaact
     1681 cgtgaagtat ggccattctt cgctcttggt ttcgttctcg ctgctgtgtc agatattact
     1741 ctaatcggat tcggtgctat cggtgttgct atcgctctta tctaccttca cctttctaaa
     1801 actggtggaa atggtggcgg aggagccgca acttctaacg acccaatcgg cgatatccta
     1861 gaagactact aagataagaa aggactgaaa acatcatgac tgaaaaactt caattaacta
     1921 aatcagatcg taaaaaagtt tggtggcgtt caaccttctt acaagggtct tggaactttg
     1981 aacggatgca aaacttgggc tgggcttata cactcattcc agctatcaaa aaactctata
     2041 ctaaaaaaga agatcaaatc gctgctcttg agcgtcacct tgagttcttc aacactcatc
     2101 catacgtagc tgctccagtc atgggggtta ctcttgcgct tgaagaagaa cgtgctaacg
     2161 gtgtggaaat cgatgacgct gctatccaag gggtcaaaat cggtatgatg ggacctcttg
     2221 ctggtatcgg tgacccagta ttctggttta cagtacgccc aatccttgga tctctcggtg
     2281 cttcacttgc ccttactggc aatatcttgg gtccactcct cttctttgtt gcatggaact
     2341 tgattcgtat gtcattcttg tggtatgttc aagagattgg atacaaggct ggatcagaaa
     2401 tcactaaaga tatgtctggt ggtatccttc aagatatcac taaaggagct tctatccttg
     2461 ggatgttcat tcttgctgtc cttgttcaac gctgggtaaa tattaaattt gctttcgatg
     2521 tttctaaagt tcaactagat gaaaaggctt atatccattg ggataaattg ccagaagggt
     2581 ctaaaggtat ccaagaagca ttcgcacaag taggacaagg attgtctcaa actcctgaaa
     2641 aagttactac tttccaacaa aacttggata tgttgattcc tggattatca ggactactcc
     2701 ttactttact ttgcatgtac ttacttaaga aaaaagtatc tccaatcact attatccttg
     2761 ccctcttcgc agtgggtatt gtggcacatg ttcttcacat catgtaatca agcaactaaa
     2821 aaggaaccag gttctaaaat ctgattcctt ttttctatgc ttttattcag ccaaggctcc
     2881 cattggatcc catggtgcaa gtacaattgg ttctgctcca taggcagctt gttcttctgc
     2941 tgtcagcaat tccttacgaa caacgatttg gtatgtgtat tcgtccatcc aagcgtctga
     3001 ggcaacaaag taaccatctg taccgacctt gtctccccat gagttttcaa ccttccactt
     3061 ggttgattta ccattttcgt ccaagtcaac acctgtcaag accatggcgt gggtcatcaa
     3121 gctttcgcta tagtccaaac gtccagcctt gtcttgagta agtttaatgt ccatgcttga
     3181 ttcaaagtca taaacatctg tcgcaaggat gccagcctta cggttactga gctggccgac
     3241 atcagaacca aaccaaacag tctcacctgc ttgcatttgg gcaatcgcca attctttcaa
     3301 gcgctccatt ggaacgttga tgtaacgaac tgcacggcta ccaaccacat tccccaacat
     3361 ctcaactgtg taagattttc cgtaaggttt atcagcagtt ggagcattga taacagaaac
     3421 gtagtcttct aaaggaagat tgacatattt cttgtaaaac tcttgtggtg tgattccttt
     3481 ttcacttttg tagttgttat ctttatcgcg ataagcaaag tcaaacttgc gtggtggaag
     3541 tcctaatgac atagcaagaa agttaaagat ttcttgcaag aggtcttctt tcttagcttg
     3601 aacagtcgct tgatctgcac cagaaacaag caagtcacgc aagatttgag catcttgacg
     3661 aagcaattta ttaaggatcg catttagctc acgactgctg ctagatgaaa cagactcagg
     3721 ataaactgac ttaggcacga caccgtattt ttcaaagagg gaaacgacca tatcccattg
     3781 accaccatct tgttgaggtg tttggagtaa gaagctaacc ttgcggctag tcaattcttg
     3841 gtctgaagtc gcaatgactt gctccaagaa ccagtttgat ttctcatact tatcccagaa
     3901 gaaagtgtgg gcttgtgaca actcaaagtt ctccaatttg tattgcgaga tgagtttgtg
     3961 gcggaaggtg ttgagagccg caaacatcca gcaacgacca gacgctttct ggttagtgac
     4021 cttgtccttg gttaaatcca atgagaaaac aggtgtgttg tctacatggc tttggcgacg
     4081 ttccagagct gcaaaaattc cgttgtggct agcagcattt tcaatcgctt ggtattttac
     4141 atttgcttca tagttggcaa atagtttatc agtaaatgat tcttgaatcg cgttcataga
     4201 ttcctccttt tattctacag tctattataa ctcttttcac aaaggaagca actaaaaaac
     4261 ataaaaggta aggacattct ccctcacctt tttcgaatat atactcttcg aaaatctctt
     4321 caaaccacgt caacgtcgcc ttgccgtact caagtacagc ctgcggctag tttcctagtt
     4381 tgctctttga ttttcattga gtattaaact aaattaaata atattagcgc ggagaatttc
     4441 taattcttcc ttggtcaagc gacgccattc ccctcgttcc aggttctcat ctaatactaa
     4501 agttcccata gtcaatcgtt gcaagtccac cacttccttg ccacagtagc ccaccatacg
     4561 cttgatctga tgaaacttcc cttctgcaat ggtcacacgg atttggcttt gattcttttc
     4621 tgtatctatg gatacaagct ccagtatagc gggtttacag gtaaagtctt tgagaggaat
     4681 accctcagca aatgtctcca catcttcttg ggtcatgatt cccttgactt gtgccagata
     4741 agtcttgtcc acatgacgct tgggcgaaag aagaacatga gccagctgac catcattggt
     4801 caagagcaaa agaccatgcg tgttaatatc caagcgtcct actgggaaaa cttccttact
     4861 ccgcgccaag tcatccaaca agtccagaac ggttctgtgc ttgggatcct cagtcgctga
     4921 gataactcct ttgggcttgt tcatcatgta gtagacaaac tcttcatact ccaacacttg
     4981 cccatcaaag cgaatctcat ctattttttc atcaatctgc aatttagctg atttttcttt
     5041 tttaccattt acagtcacgc gcccagcctt gagcaagttt ttgacctcag tccgacttcc
     5101 caccgcacag gcaactaaaa atttatctaa tctcatagaa ctattatatc atatcaaaag
     5161 gaggctggta caatgaccaa cctccttttc gtttcatact cttcaaaaat ctcttcaaac
     5221 cgcgtcaacg tcgccttgcc gtatatatgt tactgacttc gtcagttcta tctgcaacct
     5281 caaaacagtg tttttagctg acttcgtcag ttctatctgc aacctcaaaa cagtgttttt
     5341 agctgacttc gtcagttcta tctgcaacct caaagcagtg ctttgagcaa cctgcggcta
     5401 gtttcctagt ttgctctttg attttcattg agtatcagat ttaggaaatt aacttcctcg
     5461 tctccaaaaa atagctaaga caatcatggc acctaaaaca gctgggataa tagctgttcc
     5521 tgataaaact ggcccccaag ttccaaagag caagtgacct agaaaggctc cgatccaacc
     5581 gagaaacatt tttccaaaac atcccattcg ctctccacga ttggtcatag cacctgctaa
     5641 aaatcccact aggagaccaa cgaacatact tcctaacata ttatctcctt aatttgccca
     5701 attcccatta cggaaaagag gtacccgcgt tccatcctca cgaataccat cgatatccat
     5761 ttggttagaa ccaatcataa agtctacgtg aacatctgaa cggttaagcc ctgcagcttc
     5821 aagctcctct tcgctcatct ccgctccatc aacaaggcta gtcgcatagg ctgcaccgat
     5881 agccaagtgg tttgacgcat tttcatcgaa aagggtgtta aagaaggtaa tgcctgactg
     5941 agaaattgga cttggatctg gtaccaaggc acattcaccc aaggcacgcg cacccgcatt
     6001 ttcaaagaca aggtctttca taacctgatc acccttctca gcagtgatat ctacgatttg
     6061 tccatcctta aagatcacct taatgccttc aatgatattt ccgttgtagc taagcggttt
     6121 tgtagaagtg acataaccat ctgcacgacg gaagtcaggc gctgtgaaga cctcttctgt
     6181 cggcatattt ggcaagaatc cttcgccctg tgcattgaca gcaccagctg attcccaaac
     6241 gtggttcttt ggcaaaccaa gtgttaaatc tgttcctggc gctgtgtagt gaagggctga
     6301 aaattgctcc ttattaagca tatcggcctt gctcttgaga atggctgcat gctcttccca
     6361 ggccttaaca ggatctgctt cgtagacacg gcaagttttg aagatttggt cccaaaggag
     6421 atcgactgct tcttcgtcgc tcgcagcatt tgggaagact ttcttagccc actcaagtcc
     6481 agcagcggct gctacagtcc agctaacctt gttagattga gttgcgattc gcattggctt
     6541 catggcaagt cccatagctt tagcagaagc tgaaagcttg tcagcgtcca ctccgttcaa
     6601 ggcacctgga tcagatgaac gaactccaag acggctagcc ttgttctcca agagatagtt
     6661 catctcagca atcttgtatt ctggcacatt gtccaaacgc tccatcgggg catggaggaa
     6721 tttctcacgg ttaatcacat catctgtcca ctgaacgatg acctcatgcg cacccaaggc
     6781 ataagcttct ttcacgatta gatgtgccaa ttcacgttgc tccacatcaa tagagagagc
     6841 caaagtgtga ccaggttgca cgttaattcc gttcgcaacc aacaatttcg catatttttc
     6901 tagattttct ttaaaatttg gtaaaaccat cctgttttct cttttctatt tttatttttc
     6961 tttcaaagca gaaaagattc ctgctaaagc aatagcacct gacaagagtg ctgtcgataa
     7021 aagaattgta cgatcagcaa gttctaattg atattcaaat accttcttag ccggcttatc
     7081 ttccgcaaat attcttagtt tcatgagaac ccccttaaaa tagattaaaa taaatcactg
     7141 tgactgcctg tcctcagcaa atttaaaatc aattccgact tatctacttt ataaaccaaa
     7201 agccaatctg gctgggtatg gcattcacga actccttgaa aatgcttgga tgccgtcaat
     7261 gaatgatcac gatatctggc aggatgttct ttttcttgaa ccagaaaatt caaaacttct
     7321 tctaataatt ctgccttcaa accacgcttc atagccaact taaaatcttt tttaaactgt
     7381 ttatgataac gaatcttaag cacgtaagtc ctccatcaaa tctgagactg attcaaaaga
     7441 ctggctcata ttacgatttt ggtctaaatc cgttaacact tggagcaact tccgattttc
     7501 gtctagtcta acatcaaaag gtaatccctg atattgaatt gcctgacgaa ggaaaatatt
     7561 aatagctgtt gtcatatcca ttcccagatt actaaacacc tgttgggcct gctccttaac
     7621 ctcactatcc agacggatgc tcatactcat ctttgacata ctctcaccct ctttccatag
     7681 actattttaa caaaaaagaa agctaatgta aatctattgg atatacgtta gcctcttcta
     7741 atagattatt aagcaatttt ttaaaacaac tcatcaaaca aactcaactg gttatcctct
     7801 ggcatatttc caagaatacc catctcatcc atcttttcaa ccaaggttga tgagagtcca
     7861 ccacgcttgc gtagttctgt tttagagagg aattctccct cttcacgcgc ccgcaccagt
     7921 tgcttggcaa cgttctctcc cagaccatcc attgctacaa atggtgggat aagggtatcc
     7981 ccgtcgatga ggaactctgt cgcctgacta cggtagagat ctaatttgcc aaacttgaaa
     8041 cctcgttccc acatctcatt gacaatctca agagttgtat agagatcgat ttccacatta
     8101 gaggcttcat tgttcttccg tttttcagag atttcttcca ttctgcgctt gatggcctcc
     8161 aagcccgcac ccatggtctt gatatcaaaa gccttagcac gaatggagaa gtaagcacag
     8221 tagtaataaa taggatggtg aaccttgaag taagctacac gcaaggccat cataacgtag
     8281 gctgccgcat gggccttagg gaacatgtac ttaattttcc cacaggattc gatataccac
     8341 tctggcacct tattagcctt catggcttcg atatagccat ttctctcctc ttctgaaatc
     8401 tttagccaca aacccttacg tacccgttcc ataatggtaa aggccatctt aggttccaga
     8461 cccgcatgca tgaggtaaac catgatgtcg tcccgacaac cgataacagt cgataggtcc
     8521 gctattcctt gcttaatcag atcctgagca ttccccaacc aaacgtcagt accgtgggac
     8581 agaccagaca gctgaagcaa ttccgcaaag gttgtcggat gggtttcgtc taccattcca
     8641 cgtacgaaat ttgttccaaa ctctggaatc cccaacatac ccgtaggcgt tccaatttgt
     8701 tcaggtgtta cccctagcac atcagtccca gaaaagagtg ccatcacgcc ttcgtcatcc
     8761 ataggaattt cattagggtc aataccagac aaatcctgaa gttttcgaat catggtcgga
     8821 tcatcatgtc ccagtacatc gagtttgagg acgttctcat cgatatcgtg gaagttaaag
     8881 tgagtggtct gccattcagc cgtgacatca tctgctggat actggacagg cgtaaaatcg
     8941 tagacatcca tgtagttcgg aataacaacg attccccccg ggtgttggcc tgttgtccgc
     9001 ttgacacccg ccgctccttg agcgaggcgt tctacttctg catcacgata aaacttgcca
     9061 taatctcgct cgtaaccctt gacaaatcca taggcagtct tggcagctac cgtaccaacc
     9121 gttcccgcac ggaaggcata ttcttcacca aagatatcac gcacatccaa gtgggcgcta
     9181 ggctgatctt ctcccgagaa gttcaagtca atatcaggaa ccttatcccc atcaaagcca
     9241 aggaaggtct caaacggaat atcctgtccg tttttactga gtttgtgacc acagtttgga
     9301 cagtccttat ggggcatatc aaatcctgaa ccgtacgaac catctgtgat aaactcactg
     9361 tactgacact gaccacagac atagtgagga gagagaggat tgacctccgt aatcccaatc
     9421 atggtcgcaa cgaaactaga tccgacagac ccacgagaac caaccaaata accccgttca
     9481 ttagaacgtt gcaccagcat ctgcgatgcc agataaatca cagcaaatcc attccccagt
     9541 atggatgtta attctttttc aatccgcaaa tcaacaatat ctggcagcgg atttccataa
     9601 atctcaaaag ctttcttata ggtcaactca gcaactgttt cttcagcctt gtcgatgaaa
     9661 ggcgtataca agtcaccctt aacgacttca acgggttcaa atatttctgc caaggcatta
     9721 gtgttttcaa taaccagttt acgagccagt tcctctccca aaaaggcaaa ttcatccaac
     9781 atctcattag tcgttcgaaa atgagccttt ggaagtggtg ctggttgggc atgttcacca
     9841 tgaccgatag ttcgattaat catcgcaccc tgtcccaaac tacggacaat aatttcacga
     9901 taaatctctt cttccggttc gatatagtga acatttcccg tagccagaac aggcttgcca
     9961 aggcgatctc caacctctat caaactcttg ataatggtct ggagttcctc catatccttg
    10021 acctgctctt tggcaatcaa gggcgcatag atagccggtg gcatgacctc gataaagtca
    10081 taatacttgg ccacctcaac cgccccatcc acaccttgag aaacgaccac gtcaaaaact
    10141 tcaccctctg aacaggctga acctaaaatc aagccctctc gatgggcatc tagaaccgtt
    10201 ctcggaatcc gtggcactcc ttcaaaatac ttggtattag acaaggaaac cagcttaaag
    10261 atatttttta gacctacctg attcttgaca tagatggtcg catgcttgat ccgagctttt
    10321 ttgtaagaat ctggactgat tagatcaatg ttgagtctag ctaaatcggt cacaccatgt
    10381 ttttctgcta cctctttgat aaagatgaaa agcagacgac cagtcgcttc cgcatcgtaa
    10441 ttggccatgt ggtgatgttc caaggccaca ccaaaacgct tggtcaaagg ccccaaacca
    10501 tggcgtttat actcaggata gaggtttcta gcaaactcca gcgtatcaat aactggctga
    10561 ctaatctttg gaagaccatg acgctcataa ttagcattca taaagccaac gtcaaaggta
    10621 gcattgtggg caactaggac cgtatccttg caaaattctt ggaattcttg caaaacttgt
    10681 tctagtggtt tggcattttt gacatgatca tctgtaattc cagttaactc tgtagtaaag
    10741 gctgacaagg gatgcccagg attgataaat tcatcaaatt cagcaataac attccccttg
    10801 tacatcttag aggccgcaac ctgaatcaag tcattataga tagctgaaag tcccgtcgtt
    10861 tccacgtcaa agaccacgta ggttgcttct gacaagtcca tctccacttc gttatagacg
    10921 atagggacac ggtcctccac gatattggct tccatcccat agatcagctg gattcccgct
    10981 ttcttagccg ccttatagcc atgtggaaag gactggacat tcccatggtc cgtgatagca
    11041 accgccttgt gtccccactt agcagctgtt gcaacaatct cttcgacctc tggcaaagca
    11101 tccatagtcg acatgttagt atgagcatga aactcaaccc gacgctcacc ttctggcatc
    11161 aaatccttcc gctcatagtg aacaacttcc tgcagatcct gtacgttcat agtcaaatcg
    11221 cgtgtgaagt tattcatctc cacattccct cgaactcgga gccaagaatt cttcttgatg
    11281 agatcaaact tctgggcctc ttcctcgttt ttaacccact tttgcataga aaaacttgaa
    11341 gtgtagtccg tcattttaaa gttgattaaa acacgacctg ttctagtcac tttttgctcc
    11401 acatcaaaaa caaccccttc aaataccaga cgattttcct ctgtcgtcac ttcgatcata
    11461 ggagtaatct ccgccttatc cagcttgggt ttagctgcag cttttttcgc ttgaaaatca
    11521 aagactggtt tctcttccgc tggaggaggt gccatctgct ccagttgttc catagcacgg
    11581 agcgcttcct cattggcagc ttgaacaatc tgctcatttt cagcatgaaa ggcctcttcc
    11641 tgctcttggg tcaggacatc attcttatcg acttgacagt taaaagttgg aaaaccaaac
    11701 ttttcaagtt gtttggctaa attaggaaga tgattcttct taaaatgttc cttatcaatc
    11761 gcctcagatc cttcaataaa tagctgatta ccctcagcac gaacttgcaa attttgataa
    11821 agggacttaa aaccttgact agcacatgga ccttcagaga aagcctccct atagtaggac
    11881 tgcaagagct gatttgaaaa ttcttgagac cgagccttaa tttcaaaaac agctttattg
    11941 cctgtcttag aaaattcttc gctcaaacct ttctttaatt ctaaaaagat ttcaatcggt
    12001 aaaatattag aaaatacgaa atgaaactcc cataccttac taattttatg aaccacaact
    12061 cgctcaatat tggcctgtgc taaagcagga gcctgtctca tttcagcagg catccccaat
    12121 tgattcatca aaatttcaaa actatttgac attcattttc ctcacattat tcttctacta
    12181 ttttaccata tttagaggta ttttctaaag acaaaaggaa gccactaagt gacttccttc
    12241 tagagtgagg acggattagt cttcaccttt atttttctta ataatttctt cttgtactga
    12301 cttaggtaca tcttcgtagt ggtcaaatac catcatgaat gtaccacgtc cttgagatgc
    12361 agaacgaaga actgttgcgt aaccgaacat ttcagcaagt ggaacgtaag cacgaacgat
    12421 ttggctgtta ccgtgtgctt ccataccatc tacacgtcca cgacgagcag ttacgtgacc
    12481 cataacatca ccaaggtttt cttctggaac agtgattgtt acaagcatca ttggttcaag
    12541 gatagctggt tgtgctgatt tagcagcttc tttaagggaa agtgaagccg caatcttgaa
    12601 ggcagtttca gatgagtcga catcgtgata tgaaccatca taaagcttag ctttaacgtc
    12661 aaccattggg taacctgcaa gaacaccgtt agccatagat tctaccaaac ctttttcaac
    12721 cgctgggata aattcacgag gaaccacacc accgacgatt gcgttttcga attcgaatcc
    12781 tttaccttct tcgtttggag taaattcaat ccacacatca ccgaattgac ctttaccacc
    12841 agactgacgt ttgaagaatc cacgtgcttg agtagaagcg cggaatgttt cacggtaaga
    12901 tacttgaggc gcacctacgt tcgcttcaac tttgaactca cgacgcatac gatcaacaag
    12961 gacgtcaagg tgaagttcac ccatacctga gataactgtt tcaccagttt caacgtttgt
    13021 ttcaacgcgg aatgttggat cttcttcagc caatttttga agggcgatac ccatcttgtc
    13081 ttggtcagct ttagattttg gctcaaccat caattggata actggttctg gaacgttgat
    13141 tgactcaagg atgattttag atttttcatc tgtcaatgag tcaccagttg tagtatcttt
    13201 caaaccaacg gcagcagcga tatcacctga gtaaacagtg tcgatttctt gacggctgtt
    13261 agcgtgcatt tgaaggatac gtccgatacg ttcacgttta cctttagaag tattcaatac
    13321 gtatgaacct gattgaagaa cacctgagta aacacggaag aatgtcaaac gacctacgaa
    13381 tgggtcagtc atgatcttga aggcaagagc tgcaaatggc tcttcgtcag atgctggacg
    13441 agtttcttca gcgtctgtat ctgggttaat acctttgatt gctgggatgt caagtggact
    13501 tggaaggtag tcgataaccg catcaagcat caattgaaca cctttgtttt tgaaggctga
    13561 accacacaat actgggaaga attcaacgtt gatagtcgct ttacggatac cagctttcaa
    13621 ttcttcgtta gtgatttctt caccttcgag gtatttcatc atcaattctt cgtcagtttc
    13681 agcaactgct tcaatcaatt tttcacggta ttcttgagct tggtcaaggt attcagctgg
    13741 gatgtcttct tcaaggatat ccgtaccaag gtcgttagta tagatttcag ctttcatctt
    13801 gattaagtca atgataccac ggaagtcatc ttcagaaccg attggcaatt ggattgggtg
    13861 tgcatttgct tgaaggcgat cgtgaagtgt gcttacagag taaaggaagt cagcaccgat
    13921 tttgtccatt ttgttggcaa atacgatacg tggaactccg tactcagttg cttgacgcca
    13981 aactgtttca gtttgaggct caacacctga ttgtgagtca agaacggtaa ccgcaccatc
    14041 caatacacga agagaacgtt gtacttcgat tgtgaagtcc acgtgtcctg gtgtgtcgat
    14101 gatatttacg cggtggttgt tccattgagc tgttgtcgca gcagatgtga tcgtgatacc
    14161 acgttcttgc tcttgctcca tccagtccat ttgtgacgca ccttcgtgag tttcaccgat
    14221 tttgtggatt ttaccagtgt agtaaagaat acgctcagta gttgttgttt taccggcatc
    14281 gacgtgagcc atgataccga tattacgagt tttttcaagt gaaaattcgc gtgccatgag
    14341 gtttgtttct cctatttatt tttgatttct attctattat aacacgattt taataaaaac
    14401 ggataggcag gacctacccg ttctcaatgt tttcatgcta ttgttggttt caacttacga
    14461 gatggtaagc tgagttatag ctaatactaa tcgatttagc taatttgaac ccgggctaaa
    14521 gttagttagc cgatgtgagc tggaacggga tgctgcgtga aaaagataaa actccttgta
    14581 ttcgtcgaat actgcgtcag tttcctattt tcaccttgca tccttaccgt tcctagcatc
    14641 atgatttagt tgagctcaag ctaaaagtct ggagaaaaag atgaatctca ttggttgcaa
    14701 ccgcaacctg cttcgattcc ctattttctc tgggactttc ttaacgcttt cgcatcctat
    14761 cttaccaacg gaagtgtgcg aatgcacggt tagcttcagc catacggtga gtatcttcac
    14821 gtttcttaac tgctgcacca gtgttgttag cagcatccaa gatttctttt gcaagacggt
    14881 cttgcattgt gtgttcacca cgaagacgag cgattgttac caaccaacga agtccaagtg
    14941 ttgtacgacg ttctggacga acttcaactg ggacttggta gttagaacca ccaacacgac
    15001 gtgcacgtac ttcaagtaca ggcatgatgt tttccatagc tgtttcaaat acttcaagtg
    15061 catcgttgcc agtagcttct ttgatttgct caaaagcacc gtaaacgatt gaagcagcag
    15121 taccacgttt accatcaagc ataacgcggt tgataagacg agtaactagt tgtgaattgt
    15181 atagcggatc tggcaatacg tcacgttttg gagctctatt tttacgactc atttctcttt
    15241 atcccctttc cttatgcttt tggacgttta gtaccgtatt tagaacgacc ttgtttacga
    15301 tcgttaacac ctgcagtatc aagtgcacca cggacgatat ggtaacgtac ccctggaagg
    15361 tcttttacac gtccaccgcg aagaagcacc acgctgtgct cttgcaagtt gtgtccgata
    15421 cctgggatgt aggcagtaac ttcgataagg ttgctcaaac gtacacgagc gaatttacga
    15481 agggctgagt taggtttttt aggtgtcatt gttccaacac gagttgcaac accacgtttt
    15541 tgtggtgaag aaacgtttgt ttgaactttt ttatgactat tgtaaccaac gttcaaagct
    15601 ggtgatttag atttttctac ttttgattta cgcggtttgc gaaccaattg gttaattgta
    15661 ggcatctaca ttctcctgtg tttttttatt tttggtgatg atacacttgg tgacagatat
    15721 catctgtgtg tacttttgca acatttgtca gcacgtccct gtacactctt gagagaccaa
    15781 aagtaaaaag taccgtctat tattgtaaca aaattttccc ttgtttgtca agatattttt
    15841 cttttttatt ggtaatgtta gagatcaatt tctgaataaa aatagctggg aacacatcat
    15901 cttcctaata atacggcttg gcttatgtga catttttatc tccgtcattt atactgatat
    15961 ataaaatagt acagaaaact tctcaaaagt aagaattttt ttcacaaatc aaagaaaacg
    16021 cttgcatatt gttttactac atgctatact gatatagaaa tttattgatt ggagaatcat
    16081 tatgagaaaa acaccatctc acactgagaa aaaaatggtc tacagcatcc gttccctcaa
    16141 aaatggaact ggttctgtcc ttatcggcgc aagccttgtc ttgcttgcca tggctacacc
    16201 aactatctca tccgacgaaa gtacaccaac cactaacgaa cccaacaaca gaaatacaac
    16261 cacccttgcc caacctctta ctgatacagc agctggctct ggtaagaacg aaagtgatat
    16321 ttcttcacct agaaatgcaa acgcttccct agagaaaaca gaagaaaaac ctgcaacaga
    16381 gccaactact tctactagtc cagtaacaac tgaaactaag gcagaagagc ccatcgaaga
    16441 taactacttc cgtatccatg tcaaaaaact tcctgaagaa aacaaggatg ctcaaggact
    16501 atggacttgg gacgatgttg aaaaaccatc tgaaaactgg ccaaacggag ctttgtcctt
    16561 caaggatgcc aagaaagatg actacggcta ttacctagat gtcaaattaa agggagaaca
    16621 agccaagaaa attagcttcc tcatcaacaa tacagctgga aaaaatctaa ccggcgataa
    16681 atctgtagaa aaactagttc caaaaatgaa cgaggcttgg ttagaccaag attacaaggt
    16741 tttctcttac gagccacagc ctgcaggaac tgttcgcgtc aactactacc gcacagatgg
    16801 caactatgac aagaaatctc tctggtactg gggagatgtg aaaaatccaa gtagcgctca
    16861 atggcctgac ggaacagact ttacggctac aggcaaatat ggccgctata tcgacattcc
    16921 tcttaatgaa gccgcaagag aatttggatt tttattacta gatgagagca aacaaggaga
    16981 cgacgtgaaa atccgtaaag aaaattataa gttcacagat ttgaaaaatc atagccaaat
    17041 tttcctaaaa gacgatgatg aatcgattta cacaaatcca tactatgtcc atgatatccg
    17101 tatgacagga gcccaacacg taggcacttc tagcattgaa agtagctttt caacacttgt
    17161 cggtgctaaa aaagaagata tcctcaaaca ctccaacatc actaatcacc taggaaacaa
    17221 ggtaactatt accgatgttg caatcgatga agctggtaag aaagtgacct acagcggaga
    17281 tttctctgac acaaaacatc cttatactgt tagctacaat tccgaccaat tcactaccaa
    17341 aacaagctgg cacctgaaag atgagacata cagctatgat ggcaaactgg gagctgacct
    17401 aaaagaagaa ggaaaacaag ttgatttgac cctttggtca ccaagtgctg ataaggtttc
    17461 tgttgttgtc tacgacaaga atgaccctga caaagtagtt ggaactgtcg ctctcgaaaa
    17521 aggggaaaga ggaacttgga aacaaactct agacagcaca aacaaactcg gaatcacaga
    17581 tttcactggc tactattatc aataccaaat cgagcgtcaa ggtaaaactg ttcttgcact
    17641 cgatccttac gctaaatctc ttgctgcttg gaatagcgac gatgccaaga ttgacgatgc
    17701 ccataaagtg gctaaagccg cctttgtaga tccagctaaa ctcggacctc aagacttgac
    17761 ttatggtaag attcacaatt tcaagactcg tgaagacgcc gttatctacg aagctcatgt
    17821 gcgtgacttc acttcagatc ctgccattgc aaaagacttg accaaaccat ttgggacttt
    17881 tgaagccttc attgaaaaac tagactatct caaagacttg ggtgtaaccc atatccagct
    17941 ccttccagtc ttgtcttact actttgtcaa tgaattgaaa aaccatgaac gcttgtctga
    18001 ctatgcttca agcaacagca actacaactg gggatatgac cctcaaaact acttctcctt
    18061 gactggtatg tactcaagcg atcctaagaa tccagaaaaa cgaatcgcag aatttaaaaa
    18121 cctcatcaac gaaatccaca aacgtggtat gggagctatc ctagatgtcg tttataacca
    18181 cacagccaaa gtcgatatct ttgaagacct agagccaaac tactaccact ttatggatgc
    18241 cgatggcaca cctcgaacta gctttggtgg tggacgcttg gggacaaccc accatatgac
    18301 caaacggctc ctagttgact ctatcaaata cctagttgat acctacaaag tggatggctt
    18361 ccgcttcgat atgatgggag accatgacgc cgcttctatc gaagaagctt acaaggctgc
    18421 acgcgccctc aatccaaacc tcatcatgct tggtgaaggt tggagaacct atgccggtga
    18481 tgaaaacatg cctactaaag ctgctgacca agattggatg aaacataccg atactgtcgc
    18541 tgtcttttca gatgacatcc gtaacaacct caaatctggt tatccaaacg aaggtcaacc
    18601 tgcctttatc acaggtggca agcgtgatgt caacaccatc tttaaaaatc tcattgctca
    18661 accaactaac tttgaagctg acagccctgg agatgtcatc caatacatag cagcccatga
    18721 taacttgacc ctctttgaca tcattgccca gtctatcaaa aaagacccaa gcaaggctga
    18781 gaactatgct gaaatccacc gtcgtttacg acttggaaat ctcatggtct tgacagctca
    18841 aggaactcca tttatccact ccggtcagga atatggacgt actaaacaat tccgtgaccc
    18901 agcctacaag actccagtag cagaggataa ggttccaaac aaatctcact tgttgcgtga
    18961 taaggacggc aacccatttg actatcctta cttcatccat gattcttacg attctagcga
    19021 cgcagtcaac aagtttgact ggactaaggc tacagatggt aaagcttatc ctgaaaatgt
    19081 caagagccgt gactatatga aaggtttgat tgcccttcgt caatctacag atgccttccg
    19141 acttaagagt ctccaagata tcaaagaccg tgtccacctc atcactgtcc caggccaaaa
    19201 tggtgtggaa aaagaggatg tagtgattgg ctaccaaatc actgctccaa acggcgatat
    19261 ctacgcagtc tttgtcaatg cggatgaaaa agctcgcgaa tttaatttgg gaactgcctt
    19321 tgcacatcta agaaatgcgg aagttttggc agatgaaaac caagcaggat cagtcggaat
    19381 tgccaacccg aaaggacttg aatggactga aaaaggcttg aaattgaatg cccttacagc
    19441 tactgttctt cgagtctctc aaaatggaac tagccatgag tcaactgcag aagagaaacc
    19501 agactcaacc ccttccaagc ctgaacatca aaatgaagct tctcaccctg cacatcaaga
    19561 cctagctcca gaagctagac ctgattctac taaaccagat gccaaagtag ctgatgcgga
    19621 aaataaacct agccaagcta cagctgattc acaagctgaa caaccagcac aagaagcaca
    19681 agcatcatct gtaaaagaag cggttcgaaa cgaatcggta gaaaactcta gcaaggaaaa
    19741 tatacctgca accccagata gacaagctga acttccaaat acaggaatca aaaacgaaaa
    19801 caaactccta tttgcaggaa tcagcctcct tgcgctcctt ggtctcggtt tcttactaaa
    19861 aaataaaaaa gagaactaaa ctagccctcc tatagaaaaa tcccccaagc attatagctc
    19921 gggggattaa ttttcgtaca atatttgttg tcctaataaa cttgattagg attttttatt
    19981 aagcctcttt catagcaaaa taagctcgta cttttggtgc aacttgtgtt ccgaagagtt
    20041 caatagctct cagaacctgg tcatgaggca tagaaccaag cggtatatga agcatgaagc
    20101 ggtccaatcc taaatcctct atcatgcgaa tcaatttttc ggccacctga tctggattgc
    20161 caacaaacat ggcgccattt ggccctacct gctccaaata ttgctcataa cgcaattcct
    20221 gccagtgcgg acggtctttg gaaatagcat ccaccacttg cttagtcgga tggaaataat
    20281 ctttcaccgc ctgctcacca tcttccgcaa tccaccccca agaatgggct cccactttca
    20341 agtctttgtc agcatggccc gcttcgcttc caatctcacg ataagcctga atcaactttt
    20401 taaaataacg tggattacca ccaataatag catatacaat cggtagacca gcctgagcaa
    20461 tcttcactgt tgattcgaca tgaccacctg tagctatcca caagggcaat ttgtcctgaa
    20521 ctggacgagg ataaacttct ttaccagcaa tcgtttgagt caatcgacct tgccagtcta
    20581 acttggtctt ttcattgact aactgaagca agtctaattt ctcatcaaaa agagagtcgt
    20641 agtctttcaa gtcataacca aacagaggga aagattccgt gaaagagccc cttccagcca
    20701 taatctccga tcgtccatct gacaaagcat cgatagtggc atactgttgg aacaaacgaa
    20761 tcgggtccat gcttgacaga atgctgactg cactggtcaa acggattttc ttggtattga
    20821 ctgccccagc ggccaggaca atctctgggg ctgatactgc aaaatccgcc cgatggtgct
    20881 cgccaatccc atatacgtcc aaaccaacct tgtcagccag ctcaatctct gccaccaact
    20941 ggcgaatgcg ttcagcatga ctgtaagttt gtccagtccc ttcaagctcc gttatttccc
    21001 caaatgttga aattcccaat tctaccattg tgattctcct tgtctatctc tgtacttcaa
    21061 tttgaaaaat tattctaata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
    21121 ggttgctcaa aacagtgttt tgaggttgtg gatagaacta acgaagtcag taaccatacc
    21181 tacggcaagg cgacgttgac gtggtttgaa gagattttct aagagtataa cacgaatctt
    21241 gagtacaagc aaccaatttg ctcacaagaa aaagcctaga taactagact tttttagctt
    21301 attctaccgt tactgacttg gcaaggttac gtggtttgtc cacatcgagg ccacggtgga
    21361 gggttgcaaa gtaagcgact aattgcgtcg gtacgaccat tgaaattggt gagaggtatg
    21421 gatgtacggt cgtaaggacg atatcgtcgg tatctttggc tacattctct tctgcgatag
    21481 tgaggacttt ggcaccacgg gctgcgacct cttggatatt tccacgagta tggttggcaa
    21541 gaactggatc tgacaagaga gccaaaacag gcgttccttc ttcaatcaag gcaatggttc
    21601 cgtgcttgag ttctcctgcc gcaaaacctt cacactggat ataagaaatc tctttgagtt
    21661 tgagacttgc ttccatggct acgtagtaat cttgaccacg tccgatgtaa aaggcgttac
    21721 gagttgtttc aagaagttca cgaaccttgg cttcaatggt ttctttctct gaaagagttg
    21781 attcgataga ctgagctacg attgacaatt catgaaccag gtcaaaggct tgcgctttag
    21841 cattaccatt tgcttctccg actgcttttg caaggaaggc aagggctgcg atttgcgctg
    21901 tataggcttt agttgatgcc acggcaattt caggacctgc gtgaaggagc atggtatagt
    21961 tggcttcacg tgagagggtt gaacctggaa cgtttgtcac tgttaagctt ggaattccca
    22021 tttcattagc cttgaccaaa acttgacgac tatccgctgt ttcaccagat tggctgataa
    22081 agatgaagag tggtttcttg ctgagaagtg gcataccgta gccccactca gatgagattc
    22141 caagttcaac tggtgtatct gtcaattctt ccaacatttt cttagaagca aatcctgcat
    22201 ggtaagatgt tccagctgca aggatgtaga tgcggtctgc gtcttgaaca gccttaatga
    22261 tatctgggtc tacgacaact tgaccagcct catctgtgta ggcttggatg agtttccgca
    22321 taacagttgg ttgctcgtca atttccttga gcatatagta agggtaagtt cccttaccga
    22381 tatctgacaa gtcaagttca gcagtgtagc tagcacgctc acgacgattt ccatcatagt
    22441 cttgaacttc cacactatca gccttgacga ttaccaactc ttggtcatgg atttccatgt
    22501 attggttagt ttcacgaatc atagccatgg cgtctgagca gaccatgtta tagccttctc
    22561 caagaccaat caaaagtggt gatttatttt tagctacgta gatgacttca ggatcttgtg
    22621 agtcaaccaa ggcaaaggca taagaaccac ggatgatgtg aagggctttt ttgaaggctt
    22681 caagaactga gagcccttct tcttccgcaa attttccaat caaatgaacg gctatttcag
    22741 tatctgtctg ccccttgaag tggtgacctg caaggtattc ttccttgatt tcaaagtagt
    22801 tctcaatcac cccattatgc accaagacaa aacgttctgt ctcagagcgg tgtgggtgag
    22861 cattgtcctc agttggtttt ccgtgagtag cccaacgagt atgtccgata ccagttgttc
    22921 cctcaacacc agctgtcttg gcagacaatt ctgcaatacg accaacagcc ttcaccaaat
    22981 ggttatcagc accatctagg acaaaaattc ccgcagaatc atagccacgg tattcaagct
    23041 tttcaagccc ttgaatcaaa atatcagttg catttgtgtt tccaacaaca ccaacaattc
    23101 cacacatagt atatacgaca caggcaagct gtgctttctc cttaaaattg gtatagtcta
    23161 attcatcttt tatagaatca gcaaaaacag tatatacttg tttctttcac ttgtcaagag
    23221 taaaaattgg tatagttcaa attaagctcc tgtaagcata aaaactctga ccgattggga
    23281 taatcagtca gagtcctttt taaaatccat tattatcgct taattctttg aaccagtggc
    23341 ctgatttctt cagacgacgt tcttgcgttt ccaagtctaa ttcgaccaaa ccatagcgat
    23401 ttttatagct gttgagccat gaccagcagt caataaaggt ccaaatcaag tagcccttac
    23461 agttggcacc atcttcaatg gcacggtgaa gttcacgaag atgacctttt acaaagtcaa
    23521 tacggtaatc atcttgaatc attccatctt gacggaattt ttcttcccct tcaacaccca
    23581 taccattctc agtcaacatc cactcaatat tgccataatt ttccttgata ttttgggcga
    23641 tgtcataaat cccttgctca taaatctccc aaccacggtg agaattgatt ttacgtccag
    23701 gcatcacata aggctcgtaa aaatgttctg gtaagagtgg actatctgga tgcttagcaa
    23761 atcgaggagc cataacacgc aaaggttgat agtagttcac accaaggaag tccaccgtat
    23821 tatcacgaat gagttccaac tcttcctctg tagcatcagg taaaagaccg tgttcatgca
    23881 agatttctac caactcctgt ggataagtcc ccaagacaga tggatctaag aaagattggg
    23941 cctgaaaaag ggccgcaata cgagctgcct tgacatcagc aggatgctgg ctacgtggat
    24001 aagccggtgt caagttgagg acaatcccaa tcttggaatc aggcaaaagt tcatggcaag
    24061 ccttaacagc ccggctgctg gccaattgtg tatgataggc taccttaaca gctgcctctg
    24121 catccacctt atgtggataa tgggcatcat aaaaataacc aaattctaca ggaacgatgg
    24181 gctcgttaaa ggtaatccat tgatccacta aatctccata agtctcaaaa caaaaacgag
    24241 catagtcttc ataggctgag actgtcgcct tattttccca accatcacca tcctcttgaa
    24301 gggcaaaagg taaatcaaaa tgatagagat tgactaacag acgaattcct ttagccttaa
    24361 tagcctcaaa gaccttacga taaaaatcca caccttgagg gttgactttc ccacagcctt
    24421 gtggaaaaat ccgtgaccac tgaatagaag tccgaaagac tgtgtgacca gtctctaaca
    24481 aaagctcaat atcccgctcc caattttcat aaaaagtcga tgtcttatct gaaccaatcc
    24541 cattatagta acgatttggc tccacttgga accagtaatc ccagagattg tctcccttac
    24601 cgtcaccagc tacacgtcct tctgtctgcg gtccagaagt agaggatccc cagacaaaat
    24661 cctttggaaa tcttagcata acatttacct ctttatctac tcatttctcc cattatacag
    24721 aaaaaacaag gtaaaaacta gttacatttt ttccttgttt ttcttctgat tatagttttt
    24781 atttcttgct taggatttca agcgtttcaa gcacgttatc tgcatgaacc tcaatggtgt
    24841 caccagttgc cttgatctta acttctacaa tgccatcggc cgctttttta ccaacagtga
    24901 tacggattgg aagaccaatc aagtcactat cgctaaattt aacaccgaca cgttcgttac
    24961 ggtcatctgt caagacttca taaccagctc ccatcaagct tgcttcaagt ttttctgtca
    25021 aggcttgcgc ttcttcatcc ttgacattaa cagtaatcaa atgcacatca aatggtgcca
    25081 attctttagg gaaattgatt ccccaagcgt aacggtattc accttttggc gttttgttaa
    25141 caaagaggcg agcgtgttgc tccatcactg ctgaaagaag acggctgaca ccgataccgt
    25201 aacatcccat gatgattggc acagcacgac cattttcatc caagacatct gctcccatgc
    25261 ttgctgaata gcgagttccg agtttgaaaa tatgaccgat ctcaatacca cgcgcaaagt
    25321 taaggacacc ttgtccatct ggggaaattt caccctcacg aacttcacgg atatccacat
    25381 attctgcagt aaaatcacgg cctgggttca caccagtcaa gtggtagcca tcttcgttag
    25441 caccgacaac tgcattgcga acatcttgca ccttacgatc tgcaataatt ttaatattct
    25501 ctggcaaacc aactggtcca agtgaaccaa atcctgcttg aacaacattc gccacttctt
    25561 cttcgctagc aacgtcaaag aaatctgctc ccaagtgatt tttcaacttg acttcgttga
    25621 gttggtcatt tccaactaga agggctgcaa caagctcacc atctgcaatg tagaagaggg
    25681 ttttaatcgt ttgttcttct ggaacattga ggaaagctgc aacttcatca attgatttaa
    25741 catctggcgt tgcaacacga gtaacttctt cttcagcgac aacacggttg cttggtttgt
    25801 actcgtttgt tgccatttct aagttagctg catagctaga ctcacttgag taagcaatgg
    25861 tatcttcacc agagactatc catttgagca attctgcctt gatttcttct tgcacttctg
    25921 caggaatttc gtcaaatgag gcaactgact tgtccaagac aacccagcgg tcaaggtctg
    25981 tacgagcaga tgtaatggcc ataaattctt ggctatcctt accacccatg gctccaccgt
    26041 caccaataat agccttgaag tctaaaccac tacgagtgaa aatacgctca taagctgctt
    26101 tgtactcatc ataaacacta tccaaactat catagttagc gtggaaacta taagcatcct
    26161 tcatgataaa ctcacgtgta cgaagaagtc cattacgcgg gcgtttttca tcacgatact
    26221 tgggctgaat ttgataaagg ttgagtggca attgcttgta agatttaaca gaatcacgga
    26281 caatagctgt aaaggtttct tcgtgagttg gacctaagat aaagtctgat ttttcacggt
    26341 tttttagttt gtaaaggtct tcaccatagg tttcgtaacg acctgattca cgccacaatt
    26401 ctgcactaag aagggctgga gccaacatct caacagcacc aatcttttcg aattcttggc
    26461 gcatgatgtt tttagctttt tcaatcacac ggttggcaag tggtagataa gaataaacac
    26521 ctgctgaaac ttggcgaaca taaccagcac gcaacataag agcatggctg ataacttgag
    26581 catcgcttgg catttcgcga agcgttggga taggcatttt actttgtttc ataatattcc
    26641 tcgattatct aaaaaagagt cgcataatgt cattccaagt cacagcaatc atcaagacaa
    26701 ccatgatgac cactccggcc aaggtgacat aggtttcaat ttcttgtttc aatggtttgc
    26761 ggcggatggc ttctaggata ttgagcacaa tcttaccacc atccaaggct ggaatcggaa
    26821 taagattaaa aatcccaata ttgatggaaa tcattgccaa gaagtacaag atattttcaa
    26881 ttccattttt agcagcatca ctacttgcct taaagatagc aacaggtcca cccaacttgt
    26941 tcaaatctgg ttggaaaatc agatttttca gagctgagag aattcggaga gctgagtcag
    27001 cagcagttgt aaaaccacct acaaacatgg atagaaaatc tgacttaccc cccggttgaa
    27061 cacctagaag gtaacgacct tgactatctt tgggtgtaac agtgacttgt ttgtcactcc
    27121 ccttttcaga aatagtcaca tccaaagtcg gtgccgtctt atctttggtt tctgtttcca
    27181 cagcttggat caagctttcc cagttgctaa cctcatgtga gccaatcttg gtaatttgtg
    27241 ccgtttctgg tactcctacc ttggccaagg caccttgggg catgatatgg aactgattgg
    27301 tatcaacatc tctgacacca ccctgcataa agattaaaac ccaaaaaaca acgacaccta
    27361 agataaaatt gttcatagga cctgcaaaat tggtaatcag tttgccccag atagtcgcat
    27421 tttgatattg aacatctaaa ggtgcaatcc gaacctcagt accatctgct tccacaaccg
    27481 ttgcatcgtg atccactgca aatgtttttt cttcttccag aaccaatcct ttgataaaga
    27541 gcttgtcttc aaaatcaaac tgggtcacct gcatagggag gactgtttga tccaattttt
    27601 tacctgagag attgatgcgt ttaaccttac catcatcagc aagtgtcaaa ctaacaggcg
    27661 ttcctgtctt gatttcagtt gtatcatcac cccaaccggc catgcggaca tagccaccca
    27721 gaggcaagat tcgaatggta taggccgttc catccttgcc aatgtgagca aaaattttag
    27781 gtcccatacc gatggcaaat tcacgtacta aaatccctga tttcttggca aagtagaagt
    27841 gcccgaactc gtgcaccact acaataatcc caaaaactag aataaaggtt aaaattccaa
    27901 gcatagtgtt tcctccgtct tttgattaaa agagtccaaa taagtgcatg attggaaata
    27961 caagcaacat actatcgaaa cgatccaaaa caccaccatg tccagggata aatttcccag
    28021 aatccttaac accaaaatga cgtttgatcg aactttctag taaatcacca aattgtccag
    28081 caatgctaaa gaaaatagca aagactgaca tcttgtaaat tccatatgga agagcaactg
    28141 tactgtcaac tatcataaag ataatggtta ctaaaattgc tcctaaaata ccacccaagg
    28201 caccctcaag ggttttatta ggcgataccc ttggtgctaa ctttcgtttc ccatagttca
    28261 tcccaacaag ataggcacca ctgtctgtcg cccagacgat acacaaggct aagagagcct
    28321 tgtccaaacc tgcaacacga gcatctagta aagcattaaa tccaaagccc acgtagaagc
    28381 tcatagcaag agggaaaacc gcatcctcaa tcgtataaga cttgctaaaa acggtcgttc
    28441 ctaacatgat tgaaatcaaa acactatagg caaccacatt cccatcaact ggcaaaaaag
    28501 tcaggtaatt ctccaaggga atggtcaatg caaaggttgc aaagagggtc aagaggccct
    28561 ccatcgtcat ggtctctaga cctctcatct tcaaaagttc atgcatggct agcatggcta
    28621 tgattccgat tgctatctga agcaagaggc ccccaatcat taaaattggt aggaaaatag
    28681 ccagggcaat ccctgcaaac aaggttcttt tctgtaaatc ctgggtcata tttcctccta
    28741 aactcctcca aatcggcgat gacgacgatt ataggcaaga atagcttcct gcaaggccgc
    28801 ttcgtcaaaa tcaggccata aggtgtccgt aaaataaagc tcactatagg ctccctgcca
    28861 tggaaggaaa ttgctcaaac gtaattctcc actagtacgg ataatcaagt ctgggtctcg
    28921 taagtcctta ggcaaatgct gagtaaagag atagttacca atcaattcct ctgtgatgtc
    28981 acctgggttg attttggcat ctaaaacatc ctgggaaatc aacttaagcg cctgtgtaat
    29041 ctcagcacgt ccaccatagt taagagcaaa attaagaatc aatcctgtgt tgttcttagt
    29101 caattcctca gccttggtta aagcttcaaa ggtttgctta ggcaggcggt ctgtctcccc
    29161 aatcatttga atcttaacat tattcgcatg tagttccggg acataattat cataaaactc
    29221 tactggcaag ttcatgataa acttgacttc ctgatctgga cgggtccagt tttccgtaga
    29281 aaaagcatag accgtaataa ccttgacgcc cagtttgttg gctgccttgg tcacggtttg
    29341 caatgcttcc atgcccgcct tatgtccaaa aactcgcggt tgcatacgtt ttttagccca
    29401 acggccattg ccatccatga tgatgccgat atgagcagga acctgtgtcg gaacctctac
    29461 ttccacagcc ttatctttct taaaaaatcc aaacatgatc ttattcctat tcaaaaatct
    29521 atcgtttcat tataccatat ttccccattt tcttctatca ctaagctatt tattctcagg
    29581 caccatgccc atttttcaaa aaaataagcc gcctgattgg gcgactttat ttttataggg
    29641 agattattat gaaaaagttt taggagttta agttaaggtc ttcttaactt atgaacttag
    29701 tgtacactcc ctagcttaaa gtttccttaa gtatttttaa aaatcaaatt tttccatttc
    29761 tcctgccaat ttttcttgga taaacgtgtt tgatagagtt ccattcggtc ttcattttct
    29821 aagaaatgag gagttggacg aacttgaaaa ttcaaaatat cctccaaacc ataaggtgca
    29881 tagagttcaa aatctgattc ttcattcaag cgcagtccaa ctgccgtaca ccgttctgga
    29941 tacttactca tagcatcacg agaactggta taggaagcag tgtgaggact gtgctggtgc
    30001 atatagacct gatttttcaa ttcccactgg tactgaggaa aatcctctct cagctttttc
    30061 tccagtaata aggtttcctc ataagaaaaa tctggatcaa agaaaatcac atctatatct
    30121 gtttcatgat caaaagggga tttgtctgac aaaagattcc agatgaaatt tctgacagaa
    30181 cctgctgcca actacgagtc tttcaaacca aggtctcgga tgatcgtcag aatggccatc
    30241 atatctggat tttctctaaa agcctctaag atttcttgct tatttttcac tgtattcata
    30301 acctaagtgc tcatatgcct tagcagtcgc cacccgtcca gaccgtgtcc gcatgataaa
    30361 acctttttga atcaagtaag gctcatacat gtcttcaact gtctcacgct cttcggcgat
    30421 attgacagaa agagttccta gaccaacagg tcctccactg tacatctcaa tcatggtacg
    30481 aaggattttt tgatccacat agtccaaacc ttcatggtca acatccagca tagtcaaagc
    30541 cttatcggta ataatatcat cgattacccc attccccatt atctgggcaa aatcgcgcac
    30601 gcgcttgagg agacgattgg caatacgagg ggttccacga ctacgtaggg ccaactcaga
    30661 tgctgcctca tgagtgattt ccatctcaaa aatatctgcc gtccgctcga caatttctgt
    30721 caagtcagca tgggcataat actccatatg acctgtaatc ccaaaacgtg cccgtagtgg
    30781 atttgagagc ataccagccc gagtcgtcgc accaatcaag gtaaaaggag gcaactccaa
    30841 atgaacactg cgactgcctt caccagcccc aatcataata tcgatgtaga agtcctccat
    30901 ggcactataa agcacttctt ccactgacat gggtaagcga tgaatctcgt caataaagag
    30961 gacatctcca ggctctaact cattcaaaat cgctaccaaa tcacccgctt tttcgataac
    31021 aggaccagac gtttgcttga gattgactcc cagttcattg gcaatgacaa aagccatggt
    31081 tgttttccca agccctggag ggccaaataa gagcacatga tccagcgctt catcccgcat
    31141 tttagcggct tcgataaaga tctgaagctg atccttaacc ttatcctgac caatatattc
    31201 acgtaaatac tgaggacgga gcgtgcgttc tactaactcc tcatcaccca tcatctcatt
    31261 atctaaaatt ctactcatgg ctctattata tcaaaaaaac aagccacaaa caaaaaagcc
    31321 acctgattgg gtgactccta agtttagcac ttatgtggta taatattata cggcacttct
    31381 acaccgccta cgaaaggagg tgagatagcc catgatggaa ttagtactca aaactatcat
    31441 cggaccaatt gtggtcggtg tcgttcttcg tatagtcgat aaatggctaa acaaggacaa
    31501 atagtgtcaa aaaagacctc aagcttattt ggtcgtgagc ttggggtctt ttctagccta
    31561 tgatatagaa ctagtactca attccttttt attatcccat agttcacgaa ttttgtcaaa
    31621 ggtttacatt ttcttcaacc gctgtacgac aagacggtta agattaagag aacgttaggg
    31681 attctatcaa tttcatagaa attttgattt cgtaaacgaa gagacaatct tacatgtcac
    31741 ttctcattta atacgccact actagacaag caaaatcatt attacagtag ttccagttct
    31801 tcaattaaca gtcacttaca atcaaattga gtttgaacta gctgaagcga ccacagacct
    31861 atttcttagt tatattcgct aaaaaagtcc ccgccaattc cccgaccaaa atccgaaaa
//
LOCUS       DAVLXF010000013        31528 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLXF010000013 DAVLXF010000000
VERSION     DAVLXF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.013"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(64..1491)
                     /locus_tag="VYO12_000545"
     CDS             complement(64..1491)
                     /locus_tag="VYO12_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1587197.1"
                     /translation="MKTTNKVAIYVRVSTTSQVEEGYSIDEQKDKLEAYCKIKDWKIY
                     DVYVDGGFSGANTQRPELERLISDVKRKKVDIVLVYKLDRLSRSQKDTLFLIEDVFAK
                     NDVAFISLQENFDTSTPFGKASIGMLSVFAQLEREQIKERMMLGKEGRAKNGKSMSWT
                     TIPFGYDYSKETGVLSVNPTQALIVNRIFTEYLNGKPVVKIIRDLNAEGHVGRKRPWG
                     ETITKYLLKNETYLGKVKYKDKVYEGQHEPIITQELFDLVQLEVERRQISAYEKYNNP
                     RPFRAKYMLSGLMKCGYCGASLGLRYTRKDKNGISHHKYQCRNRHSKDLEKRCESGWY
                     SKEELERGVIKELERIKFDPKYKNETLAKKEETIKVEEIKKQLERINNQVSKLTELYL
                     DEIITRKELDEKNDKIKTERQFLEEQLENQKSNVLSIRKRKLTRLLKDFDVEKLSYED
                     ASKIVKNIIKEIIVTKDGMSITLDF"
     gene            complement(1659..1859)
                     /locus_tag="VYO12_000546"
     CDS             complement(1659..1859)
                     /locus_tag="VYO12_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587198.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            complement(1861..2652)
                     /locus_tag="VYO12_000547"
     CDS             complement(1861..2652)
                     /locus_tag="VYO12_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEV1587199.1"
                     /translation="MARGRGKLTPQDKEDMKVFSANLNSILSDRNCKQAELSRATGIP
                     PSTLTGYVKGTSLPIPGNVQKIADFFGVPKSVLDPRFITNNFMVNDSSSNTPQIQTIY
                     DELEPPRQGKVLNYAKRQLKEQKNEEETKINEVSEAIRLYSYDYYDHPASAGTGQYLN
                     DVRVERIELPVDVDADFVIPIKGDSMEPDYHDGDLVFIQTSVDLNDGVIGVFNYNGDA
                     YIKQLVIDEDQAYLHSLNPEYKDMPITPDTDFRIIGEVVDLYREK"
     gene            2825..3100
                     /locus_tag="VYO12_000548"
     CDS             2825..3100
                     /locus_tag="VYO12_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587200.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEENFLYSQLEAFCTNCRQF
                     AFGWTELAEEIERQERYLLDSGFTQEEIDDIRFDAAFAGMLDKLNVA"
     gene            3175..3342
                     /locus_tag="VYO12_000549"
     CDS             3175..3342
                     /locus_tag="VYO12_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587201.1"
                     /translation="MNESFLTILGISMIASFITNLIAYLAGKHHLKKKIKNHKMWFDS
                     EIERIKKKYHL"
     gene            complement(3333..3782)
                     /locus_tag="VYO12_000550"
     CDS             complement(3333..3782)
                     /locus_tag="VYO12_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587202.1"
                     /translation="MNNFSFIETLAIAAIPAFVSGMWSYIAAKGNSKHEIDKINIAHS
                     QELENVENQFKQDMEKMQKQHSQELYSLQQTHELRLLELEKVSQLDTQTDQGLKINDL
                     IFKAISGEISADVAIKNMSTLSHFANKQQPSDLQKQFVKKLSKKNHK"
     gene            3981..4151
                     /locus_tag="VYO12_000551"
     CDS             3981..4151
                     /locus_tag="VYO12_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587203.1"
                     /translation="MKEKLNEFLKFRSQFTKREWFEINQAVEACLNEKADQLKLDDSD
                     VEIISKRLGRSI"
     gene            complement(4376..4582)
                     /locus_tag="VYO12_000552"
     CDS             complement(4376..4582)
                     /locus_tag="VYO12_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587204.1"
                     /translation="MTDYQLEASLIVLGKEFDRTKKNGKESFSVHVSFFDGLDANQHL
                     QEFARQYPVKIDRSNSDQITFLIK"
     gene            4655..4882
                     /locus_tag="VYO12_000553"
     CDS             4655..4882
                     /locus_tag="VYO12_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1587205.1"
                     /translation="MPKMTLRAIRTNYNLSAKEVADKLNIHQQTLLKYEHDSSKIPMD
                     LLDKLARLYNVEKDFIFLGKKYELNHNLGEV"
     gene            4925..5287
                     /locus_tag="VYO12_000554"
     CDS             4925..5287
                     /locus_tag="VYO12_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1587206.1"
                     /translation="MQYIIRNTHGNYTSLNNAYAQDKRLKATTIGILTVILMNKSDWV
                     VYPDEIARRLGISRRTVDEHFKLLEKAGYLRVYRLGLGRGKGVTVYRFFSDMPISDDY
                     FEYLKTNLEKELSTDNGV"
     gene            complement(5697..5888)
                     /locus_tag="VYO12_000555"
     CDS             complement(5697..5888)
                     /locus_tag="VYO12_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587207.1"
                     /translation="MKSFKDFRESLTAEDMQAISAKANEATKQIDHTDGLQLGKVSGL
                     TSVITTIELLEKYHEWLHS"
     gene            6007..6150
                     /locus_tag="VYO12_000556"
     CDS             6007..6150
                     /locus_tag="VYO12_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587208.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     KTV"
     gene            6217..7014
                     /locus_tag="VYO12_000557"
     CDS             6217..7014
                     /locus_tag="VYO12_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEV1587209.1"
                     /translation="MNMNLNTNEILTTTEYDMFRKISNRKITENPKLEEELLSEGQRQ
                     PILVNEKMEVIDGQHRLYYLRKHRKPVRYIIDPTANFKTVISMNTSAVNWALQDYVYS
                     FALEGDPEFVKLAKFLDENELLSDKMVIVAGSGRRDGTAAHVVKKLKKGDYVFSNEKQ
                     LREFCKFYERVLNETKLPNKPFLQSVLWTLYTTSVFDENRMLTQLKKSDLTSEDIEGF
                     AKKKLLLTFLELYNGRWSDDHPSLIQYFINRKGSLTIPSLPKQDEDN"
     gene            7032..7169
                     /locus_tag="VYO12_000558"
     CDS             7032..7169
                     /locus_tag="VYO12_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587210.1"
                     /translation="MKVTVYVYGRKLEPDEPIIIPENHRFYDIWNGIVNEMLDNEEDV
                     A"
     gene            7169..7390
                     /locus_tag="VYO12_000559"
     CDS             7169..7390
                     /locus_tag="VYO12_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587211.1"
                     /translation="MKLLTKLKLRLEGVLKSVNLDWREVAVELMNDLFEERKRRFAFE
                     QENYDLKQELAAYKYKENFDIKARLQGEM"
     gene            7391..7645
                     /locus_tag="VYO12_000560"
     CDS             7391..7645
                     /locus_tag="VYO12_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587212.1"
                     /translation="MYIISIYVKNTETGNEDFSLIGRDFLPTGHQDYIARVFGTKEEA
                     IDYLKSISYIASGVHGNDWVYQNEKLPEIESRCRIWKVGE"
     gene            7658..7918
                     /locus_tag="VYO12_000561"
     CDS             7658..7918
                     /locus_tag="VYO12_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587213.1"
                     /translation="MFKALKTIKKIKQLQKEMHAFSLAFLALQDMGLMPETERSKAKA
                     QTMHDVSRVLKDVLDGKSVDEAIKRLNSEVKAEEVGQKDDQN"
     gene            8188..9006
                     /gene="bet"
                     /locus_tag="VYO12_000562"
     CDS             8188..9006
                     /gene="bet"
                     /locus_tag="VYO12_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEV1587214.1"
                     /translation="MTNNQIVEAKGDFLTNPQLLNSGIIRKYLDPQGKASDEELAYFI
                     AQAKAQNLNPFTKEIYFIKYGTQPAQIVTAKSAFEKKADSHPQFDGKEAGVIYLMDGE
                     IKYSKGAFIPKGAEILGGWAKVYRKDRTYPTETEVSFEEYDNSKIRARVKELTQQGKD
                     VTYPVMNSYGKPIGENNWDTMPCVMIRKVALVSAYREAFPAELGASYEADEIQLDNTP
                     KDVTPQESREDVVARKMAQIDQFNKEQEANHADPEPAKTEEPIQGELLDGELEY"
     gene            9016..10080
                     /locus_tag="VYO12_000563"
     CDS             9016..10080
                     /locus_tag="VYO12_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEV1587215.1"
                     /translation="MQELQVIDDKKINKIYEMITTDELTRESFEKDLIEATEKYKDYI
                     PTAGTLKDDKAKRAEFNKLIESKNRIRIDTKNLLSETANTWDSYAKSIIDPFATVVSE
                     FDKGIKEIEEHQRQLKIDTVKSYLANKSAEYMLDPRLFDEKALEYVKASDFMADGVTL
                     KKATMKSLDDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTLAEVFGQ
                     IKADYEFEKQKEELRQDLERAERSNQELLAAQQTKQQEQAPKSTETPNFDPETGKILD
                     GGQILQNEPNALRGAENDLKRYAQKMTLEVYFVDTAEKDRFKAGLSQLGFDFKKNYQV
                     RGYQRIDPLTQAELNEQCGW"
     gene            10248..10571
                     /locus_tag="VYO12_000564"
     CDS             10248..10571
                     /locus_tag="VYO12_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587216.1"
                     /translation="MDIRKISDSVAIYSDGKKLQVIHNLGDEFILDFKVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEKFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            10564..10899
                     /locus_tag="VYO12_000565"
     CDS             10564..10899
                     /locus_tag="VYO12_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEV1587217.1"
                     /translation="MIKTVFFSCDYPHHEVIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            10994..11410
                     /gene="ssb"
                     /locus_tag="VYO12_000566"
     CDS             10994..11410
                     /gene="ssb"
                     /locus_tag="VYO12_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1587218.1"
                     /translation="MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFQAWRGTADIIAQYCSKGSLIGIIGRLQVRSYEKDGQRRYVTEVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            11423..11821
                     /locus_tag="VYO12_000567"
     CDS             11423..11821
                     /locus_tag="VYO12_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587219.1"
                     /translation="MEIDKIIKKDVLEFMETISDNKIDASKINVTERIAIIIEKQKIE
                     VVTTLNYDMSISFDNKDTAPTLDDNGDLFEPVYKCKVKAIPKNDVFFTSLTRVKSNTK
                     TLQEVKKFFEFVNENRENLFEMAGFKGVLE"
     gene            11818..12249
                     /locus_tag="VYO12_000568"
     CDS             11818..12249
                     /locus_tag="VYO12_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEV1587220.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRTRLRFYIKPPQYISKVKKNQQALLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            12265..12447
                     /locus_tag="VYO12_000569"
     CDS             12265..12447
                     /locus_tag="VYO12_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587221.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            12472..12789
                     /locus_tag="VYO12_000570"
     CDS             12472..12789
                     /locus_tag="VYO12_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587222.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPIDYDLGATKDWLPKSKEFDDWVGYSPSMAQVVIAVYLIY
                     GGN"
     gene            12791..13321
                     /locus_tag="VYO12_000571"
     CDS             12791..13321
                     /locus_tag="VYO12_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1587223.1"
                     /translation="MNIKALIKKYEELWNEHSPFYEPVPYTSMVELFLKELKQLDEPQ
                     KVKIPQFVADWIEVCKEHLTTSLYTAMTPNFMKENNQSFDLILWIKKASNQDLFARAW
                     LDGYEVEKEKRYLVKIKGNIKENMLVYGELLERYFFTKSFSLDDAIYSHTRKELENAK
                     IGWVFDCPGIEIEEVE"
     gene            13479..13739
                     /locus_tag="VYO12_000572"
     CDS             13479..13739
                     /locus_tag="VYO12_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEV1587224.1"
                     /translation="MKRFIAIWILLSAGLNIWQMDRIRDLEEKKPMVIYKADNAGVEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYDKVKVGDEVLL"
     gene            13736..14158
                     /locus_tag="VYO12_000573"
     CDS             13736..14158
                     /locus_tag="VYO12_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587225.1"
                     /translation="MTETIKLPDYYEPDWKNARYGSLEELKELLLFKRIVKWDKDFLL
                     LEDGTKVTIEMSESDCCASAGGEFQDVSLDAVITNIEIGEPEEIPDHWGTGYKNKVTI
                     FHNQNPVAIANCEAEHNGYYYSVCSLVIGDIHFPVVNA"
     gene            14170..14607
                     /locus_tag="VYO12_000574"
     CDS             14170..14607
                     /locus_tag="VYO12_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587226.1"
                     /translation="MTPRYRAWIKTEKRMFFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYDFDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYDKEFIG
                     IVKQLEGSWVIDTGSDAVCLWTEIEENEIIGNIYENKEFGGRK"
     gene            14604..14975
                     /locus_tag="VYO12_000575"
     CDS             14604..14975
                     /locus_tag="VYO12_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587227.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFKH"
     gene            15048..15452
                     /locus_tag="VYO12_000576"
     CDS             15048..15452
                     /locus_tag="VYO12_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1587228.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSEVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            15533..15615
                     /locus_tag="VYO12_000577"
     tRNA            15533..15615
                     /locus_tag="VYO12_000577"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15564..15566,aa:Tyr,seq:gta)
     gene            15933..16373
                     /locus_tag="VYO12_000578"
     CDS             15933..16373
                     /locus_tag="VYO12_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV1587229.1"
                     /translation="MSRLRIKQKRFADEYIISGNATEAYKKAGYRVSSDRVAGVEGHK
                     LLKNPKIKSYIDERLKQLDSEKIADQQEVLSYLTSVMRGETQEQTLISIGELGQTITD
                     IDVGAKDRIKAAELLGKRHRLWTDKVEAAVSGTVVFANESDIPD"
     gene            16351..17649
                     /locus_tag="VYO12_000579"
     CDS             16351..17649
                     /locus_tag="VYO12_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV1587230.1"
                     /translation="MSQTYQIKQSDIVIDLPKTVGAGYGQFWRSRNLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITVKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETSRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFDKDELLRDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYTIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            17661..19130
                     /locus_tag="VYO12_000580"
     CDS             17661..19130
                     /locus_tag="VYO12_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1587231.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHSTEGSHLKKLKSYYL
                     GEHAILEHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMAELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTIQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRSVR"
     gene            19039..20676
                     /locus_tag="VYO12_000581"
     CDS             19039..20676
                     /locus_tag="VYO12_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEV1587232.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEV
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPESEIKHLQSFSWVGDGSTYSTNIWKNTGKLTSSIKDELLISLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFAAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKTELVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKIIADSLVENISLSKAVSKINDALRYTTIFDSDTFTEEYLKMK
                     QKLIAEGYKIVKVKNTWPVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            20678..20887
                     /locus_tag="VYO12_000582"
     CDS             20678..20887
                     /locus_tag="VYO12_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587233.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            20884..21297
                     /locus_tag="VYO12_000583"
     CDS             20884..21297
                     /locus_tag="VYO12_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEV1587234.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVKTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
     gene            21340..21627
                     /locus_tag="VYO12_000584"
     CDS             21340..21627
                     /locus_tag="VYO12_000584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587235.1"
                     /translation="MNKYKKLIELIENNGLEIQSKKCYDPQSAWHGEELWIVDKKKQN
                     KIFDLSGNGYCFHDAKVEEAIEEVEKYLLLKKMDTFDDFKKWVEKNAKPKK"
     gene            21891..22415
                     /locus_tag="VYO12_000585"
     CDS             21891..22415
                     /locus_tag="VYO12_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV1587236.1"
                     /translation="MSEEINATVSTESTETVDTQENVDTVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQT
                     VQQEVTEKWRKDFL"
     gene            22438..23463
                     /locus_tag="VYO12_000586"
     CDS             22438..23463
                     /locus_tag="VYO12_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEV1587237.1"
                     /translation="MAFEELNTAESRKKHLGIIEDVLAVNSYSTPLVTSSDAVTLQGR
                     SFTVATGNTTELKDYKRNKDNEFDHVEVEEKVYTLDEEKYWGRFVDQLDERDSNGQVN
                     INYVIARQAAEVVAPYLDELRFGAALGNVSDNVAMGKTAGANNAYNAVLDVSEKLDEL
                     GITKERLLFVTPSFYKAIKSEIVRLPHGDADKKVLGKGYVGELDDYTVYKVPSKFLKG
                     VNALATAPGVVTSPVQVDNTKYNDNIPGRFGELVEQLLYTGAFVLEHFKKYIITIADS
                     KPAAKPSTQGKVVNRAKAWKTGTTYKEGDTVTHADKVYVAVKDISNSSTAPDTDTTNW
                     KEKTGKK"
     gene            23473..23661
                     /locus_tag="VYO12_000587"
     CDS             23473..23661
                     /locus_tag="VYO12_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587238.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVA
                     ISDVLEEIFPKPEFLEED"
     gene            23663..23974
                     /locus_tag="VYO12_000588"
     CDS             23663..23974
                     /locus_tag="VYO12_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV1587239.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTDLLQRIRMHRLARVAGHVFEA
                     E"
     gene            23955..24293
                     /locus_tag="VYO12_000589"
     CDS             23955..24293
                     /locus_tag="VYO12_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587240.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            24268..24783
                     /locus_tag="VYO12_000590"
     CDS             24268..24783
                     /locus_tag="VYO12_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1587241.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDRAIGEVYTNLKYAPYVEFGTGPIGQANHSGISPEV
                     SVTYKSNPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            24780..25148
                     /locus_tag="VYO12_000591"
     CDS             24780..25148
                     /locus_tag="VYO12_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587242.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGIVDLHSELVFQFRMEN"
     gene            25151..25564
                     /locus_tag="VYO12_000592"
     CDS             25151..25564
                     /locus_tag="VYO12_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1587243.1"
                     /translation="MLANGITLSYGETKDSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYEFGIGDIGELEYKFSYNNSSATAPYRVLRKAADDKKKLYFEQAYPDGTKVIFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSNLTFTDGIGG"
     gene            25569..25949
                     /locus_tag="VYO12_000593"
     CDS             25569..25949
                     /locus_tag="VYO12_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587244.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVDK"
     gene            25988..26338
                     /locus_tag="VYO12_000594"
     CDS             25988..26338
                     /locus_tag="VYO12_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587245.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            26325..29918
                     /locus_tag="VYO12_000595"
     CDS             26325..29918
                     /locus_tag="VYO12_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587246.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSSSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIKSTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGNTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSIATIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVVPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSLIVGGIKNLGLAIKALTFDKLVSFAETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICQAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIGNIFGNLGSWFGGRWNDSKNALAEANTWLGDKFKS
                     GRDKVNSAFEKVGSWFGDRWNDIKDGVKEADTWFGEKFESAKKKTQNPFQKIGSWFGD
                     RWKDMQDALKEIPNWFKNLFNDAMDNAKNIVKSGIDKLKSFFNFDWSLPKIKLPHFNI
                     SGSFSLMPPRIPSFSVDWYARGGVFNSPSIIGVGEAGQEAVMPLERNTGWISILAQKL
                     AERMPANNVPTGYSLPAGDIVIQIAGHEFGRVAIQEINKEHERAGQTLLKI"
     gene            29927..30277
                     /locus_tag="VYO12_000596"
     CDS             29927..30277
                     /locus_tag="VYO12_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587247.1"
                     /translation="MAQLTINGVAVKPPKSFQVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSEFFEVSYPDPMDGQVTKTFYVGARTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            30282..>31528
                     /locus_tag="VYO12_000597"
     CDS             30282..>31528
                     /locus_tag="VYO12_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1587248.1"
                     /translation="MDALTRRQFDRSMFAKERTLAIRVGEYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQGGIEAKIQAQIEAADKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKEAKRKA"
BASE COUNT        10270 a         5472 c         7094 g         8692 t
ORIGIN      
        1 aaaatatgta taataaagtt atcttaatta ctttgactaa aatacagaaa tacgctagac
       61 cctttaaaaa tctagcgtta tcgacatgcc gtcttttgta acaataattt cttttatgat
      121 atttttgaca atttttgaag catcttcgta gcttaatttt tcaacatcga agtcctttag
      181 tagccgtgtt agctttctct ttcggatgct cagaacattc gatttttgat tttcgagctg
      241 ttcttccagg aattgccttt ctgttttgat tttatcattt ttctcgtcca actctttccg
      301 agtgatgatt tcgtccaagt acaattctgt taattttgag acttgattat ttatccgttc
      361 gagttgtttc tttatctcct cgaccttgat agtctcttct ttcttagcga gtgtctcatt
      421 tttatatttt ggatcaaatt taattctttc aagttccttg attacacccc tttcaagttc
      481 ctctttagag taccaccctg attcgcatct tttttctaaa tctttgctgt gtcggttcct
      541 gcattggtat ttatgatgag agattccatt cttgtctttt cttgtatatc tcagaccaag
      601 tgaagcacca caatatccgc atttcattaa tccagaaagc atatactttg ccctgaacgg
      661 ccttggattg ttatattttt cataggctga aatctgcctt ctttcgactt caagctggac
      721 taagtcaaac aattcttgag tgataattgg ttcatgctgc ccctcgtata ccttatcttt
      781 gtactttact tttcccaaat aggtttcatt tttcagcaag tatttcgtga ttgtttcgcc
      841 ccaaggtctt tttcttccaa cgtgaccttc agcatttaag tctctgatga tcttgactac
      901 tggcttaccg ttcaagtatt ccgtgaaaat acggttgaca atgagcgctt gggtcggatt
      961 cactgataag acgccagttt cttttgagta atcatagccg aatggtattg tcgtccatga
     1021 catggatttt ccattctttg cccgtccttc ttttcctaac atcatacgct ctttaatctg
     1081 ctcacgctcc agctgagcga atactgatag cataccgatt gaagccttgc cgaaaggcgt
     1141 agaagtgtca aagttttctt gcaagctgat aaatgccaca tcgtttttag caaacacatc
     1201 ttcaatcaga aacagagtat ctttctggct acgactgagg cggtctagct tgtagactag
     1261 cactatatcg actttcttgc gcttcacgtc tgagattaaa cgctccaatt caggtctttg
     1321 agtgttagca cctgagaaac caccatcaac gtagacatcg tagattttcc agtctttgat
     1381 tttacagtag gcttcaagct tgtctttctg ctcatcgata gagtaaccct cttccacctg
     1441 cgaagtagtg gacaccctga cgtagattgc gactttattc gttgttttca ttgaatttgt
     1501 accccttttt tgataaaata ggtacaagaa aacgtctcaa aaggtaatat cttttgaaaa
     1561 gtttttcttg ccactagcct cacgctcgga gtcgccaaac tttgagagcg tggggctttt
     1621 tttgtttgca attatttcca ttttggaaac agttggtttt attctttcga taagtgttgt
     1681 tgaagaatta aggccacgtt ggctttctct tcctctgtca taggaggttc gtttggatcg
     1741 tctaccgaaa actcgatagc atgccattta tcatttactc taatccactc tcttcgtctg
     1801 tggcatttgc aatctaggtt gtgtttaatc acttccatcg gtctactttc gttactcatg
     1861 ttatttctcc ctatacaaat ccacgacttc gccgataatt cggaagtcgg tgtctggtgt
     1921 aattggcata tctttatatt cagggtttag gctatgtagg taagcctggt cttcatcaat
     1981 aacaagctgc ttgatataag catcgccgtt gtagttgaat actccgataa ctccgtcatt
     2041 caaatctaca cttgtctgaa tgaataccag gtcaccatcg tggtagtcag gctccatgga
     2101 gtcacctttg atggggatga caaagtcagc atcaacatct actggtaact caatccgttc
     2161 cactcgtaca tcattcaaat actggcctgt acctgcggaa gctgggtggt cgtagtagtc
     2221 gtaactgtag agccgaatag cttctgatac ttcgtttatc ttcgtttctt cttcgttttt
     2281 ttgctctttc agttgcctct ttgcataatt caggactttg ccctgtctag gtggttctag
     2341 ttcgtcgtag atggtttgaa tttggggagt attagaagaa gagtcattga ccatgaaatt
     2401 attagttata aatctaggat ctaatacaga tttaggaact ccaaaaaaat ctgcaatttt
     2461 ttgaacatta ccagggattg gcaaagaagt tccttttaca taccctgtca atgtactagg
     2521 cggtattcct gttgctcgag acagctcagc ttgtttacaa tttctatcag ataaaattga
     2581 gttaagattt gcagaaaaga ctttcatatc ctctttatct tgaggagtta atttccctcg
     2641 tcctcttgcc atatttttac ctcctatctt ctttactata taataccgtt tattttcgat
     2701 tttgtaaata aaaaattcga aaaaattacg aaaaaattcg aaaaatcatt gacatacgat
     2761 ttaaatcgta gtataatata atcaagctta aggaaataac aaaaacaaac cggagggaaa
     2821 caccatgaac acattaaacg agaaagcaat caacatcttt aaagcagtag ctaaggaaac
     2881 tttaatccaa ggcacttacg aggaaaactt cctttacagc cagcttgaag cattctgtac
     2941 taactgccgt caattcgctt ttggatggac agagttagca gaggagattg aacgtcaaga
     3001 gcgttacctt ctcgattctg gtttcactca agaggaaatc gatgacattc gttttgatgc
     3061 agcatttgca ggaatgcttg ataaattaaa tgtagcctga ttggtatcac caaggttcga
     3121 atccttggca ggttgttgct catagagcga aaaaagagag aaaggagtag aacgatgaat
     3181 gaatcttttc ttacgatact aggcatatca atgattgcta gttttatcac gaatttaatt
     3241 gcttacttag ctggtaagca tcatctgaaa aagaaaatta aaaaccacaa aatgtggttt
     3301 gattctgaga tagagcgtat caagaaaaag tatcatttgt gatttttctt ggataatttt
     3361 ttcacaaatt gtttttgaag atcggaaggt tgttgcttgt tcgcgaaatg actaagagtg
     3421 ctcatatttt ttattgctac atctgcagat atttctcctg aaatagcttt aaaaattagg
     3481 tcatttatct ttaagccttg gtcggtttga gtgtctagtt gagatacttt ttcaagttca
     3541 agtaatctta attcgtgagt ttgttgcagc gaataaagtt cttgtgagtg ttgcttttgc
     3601 attttttcca tatcttgttt aaattgattt tcgacatttt caagctcttg tgagtgtgca
     3661 atgttaattt tatcaatttc atgcttgctg tttcctttag cagcgatgta agaccacatg
     3721 ccagaaacaa aggcaggtat ggcagcgatc gcaagtgttt ctataaaact aaaattattc
     3781 ataagatttc tccaatcatt ttattttgat tataccacat ttgaaaggta gttagaattg
     3841 gaagataaaa tcattgaact tgctgattac ttcatcagcg aaaacacaac gtacagagaa
     3901 gctaaaatag cgtgtgagaa gctatttctt caagtcagtc atgagataga actcagggca
     3961 atggaaagta ggacgagggt atgaaagaaa aactaaacga atttctaaaa ttcagaagcc
     4021 aatttacaaa acgagaatgg tttgaaatca accaagctgt cgaagcttgt ttaaatgaaa
     4081 aagccgacca gttgaaactg gacgactcag atgtagaaat catttctaaa agactaggac
     4141 ggtctatcta aagaaagggt caaacaaatg gcgtctaaca gaactatatc agtaaataca
     4201 tcagagcatg atgtattgtt gacggcaaga aaaaaccacc ctgctgtatt cgtcgatgga
     4261 atgtttctcg acggagttga gcgagtggaa tttaccaatc attatctgga gaagtgtgaa
     4321 gttgttttaa cgtttaacga tagagttgaa accaatccct tccctctaaa cgatattact
     4381 ttattagaaa agttatttgg tcagagttcg aacggtcaat ctttacggga tattgtcgtg
     4441 caaactcttg aagatgctga ttagcatcta aaccatcaaa aaaagaaaca tgaacactaa
     4501 aactttcttt tccgtttttc ttggttctat caaattcttt gccaaggaca attagagaag
     4561 cttctaattg ataatcagtc ataacatcac ctcctttcta gctttattat agcagaattg
     4621 cgaggaacaa atagaaaaat aaggaggtag gaacgtgccg aaaatgacat tgagagcaat
     4681 aagaacaaat tataacttat ctgcaaaaga agttgccgat aaacttaaca ttcatcaaca
     4741 aacactgttg aagtatgagc atgatagttc aaaaattcca atggatcttt tagataaact
     4801 tgctcgacta tacaatgttg aaaaggattt tattttttta ggcaaaaaat acgaattaaa
     4861 tcataatcta ggagaggtgt gaatgaacga gcgagagtta tagaaagggg attaaatatg
     4921 aggtatgcag tatataatca ggaatactca tgggaattac acatctttga ataatgctta
     4981 tgctcaagac aaacgtttaa aggcaacaac gataggtatc cttacagtaa tcttgatgaa
     5041 taagtctgat tgggttgtgt atcctgacga gattgcacga cgtctaggaa taagcaggcg
     5101 caccgtagat gagcacttta agcttttaga gaaagcaggc tatctcagag tatatcgctt
     5161 agggttaggc agaggtaaag gcgtaacggt atatagattt ttttcagata tgcctatttc
     5221 agatgattac tttgagtatc taaaaactaa tcttgagaaa gagttatcca cagataacgg
     5281 agtttaaaaa tacagttgga aaatattgcc atgtgtaaaa ttgccatgtg taaaattgcc
     5341 atgtgtaaaa ttgccatgtg taaaattgcc ctctaataag tactaactat acaacaagta
     5401 ctaactatac aataatctaa gcctaacggc actaacttag taataactac taacttacaa
     5461 caaactaata cttctctaaa aaataaaaga gagaaatttc aattttagga ctttgaaaaa
     5521 taggaaagga gaaaaaatga gaccaagacg atatccgtat agtgggaaaa aagagtccac
     5581 ctttgtaaag gccgaccctg agttagttga aaaactttta agaaacacta gttatcttga
     5641 gtgtttacaa aaaaagccta tcaattttca gatagactca gaagaattta agcgtcttag
     5701 ctatgaagcc attcatgata cttctcaagt aactcaatag tagttattac agaagtcaaa
     5761 ccactgacct tccccagttg caatccgtct gtatggtcaa tctgtttagt agcttcatta
     5821 gctttagcag agatagcttg catatcttca gctgttaaag attctcgaaa atctttaaag
     5881 gatttcataa aaactcctcc tttctattgg aattttgact aaaacggtga gaggtcctag
     5941 tcgagattat tatagcaatt taggaggata ttacatcggt cttgaggctg atttttggag
     6001 gcaatattgg aagataaaat catagaactt gctgactact tcatcagcga atctacaacg
     6061 tacagagaag ctaaaatagc gtgtgagaag ctattgaaac aagtcagcca tgagatagaa
     6121 ctcagggcaa tggaaagtaa gacagtctag aagacaacaa aaagcacctg acggcaatca
     6181 ggcgcatact aaaaaacttt acaagaggat tataacatga atatgaactt aaatacaaat
     6241 gaaattttaa caactactga atatgatatg tttcgcaaaa ttagtaatag aaaaataact
     6301 gaaaatccta aattggaaga ggagctactt tctgagggac aacgtcagcc gattttggta
     6361 aatgaaaaaa tggaagttat cgacgggcaa catcgtcttt attacttgag aaaacataga
     6421 aaaccagtgc gctacataat tgatccgacc gctaatttca agacagtaat ttcgatgaat
     6481 acatcagctg tcaactgggc attacaagat tatgtgtatt cgttcgcttt agagggagat
     6541 cctgaatttg ttaaattagc taaattttta gacgagaacg aattgcttag tgacaagatg
     6601 gtaatcgtag ctggttcagg aagacgtgat ggtacggcag cacacgtagt caaaaaatta
     6661 aaaaaaggcg attatgtatt ttcaaacgaa aaacagttaa gagaattttg taagttctac
     6721 gaacgcgttt taaacgaaac aaaactgcct aacaaaccat ttttacaatc tgttttatgg
     6781 actttgtata caacatcagt ttttgatgaa aacagaatgt tgacacaatt gaagaaatca
     6841 gatttgacat cagaagatat cgaaggattt gcaaagaaaa aattgctatt aactttttta
     6901 gagctataca acggaagatg gagtgacgat catccttctt taattcaata ctttatcaac
     6961 agaaaagggt cgttaacaat tcctagtttg cctaaacaag atgaagataa ttaaaaaagt
     7021 aggaagataa aatgaaagtc acagtatatg tttacggccg aaaattagaa ccagatgaac
     7081 caattatcat cccagaaaat catcgttttt atgacatttg gaacggaatt gtaaatgaaa
     7141 tgctcgacaa cgaggaggat gtagcttaat gaaactactt actaaattaa aactcagact
     7201 cgaaggagtt cttaaatcag tcaaccttga ctggagagag gtagcagtcg aacttatgaa
     7261 cgacctattt gaggagcgca agcgtcgctt tgctttcgag caagaaaact atgacttgaa
     7321 gcaggagctt gctgcctaca agtacaaaga aaactttgat atcaaggcta gactgcaagg
     7381 agaaatgtag atgtacatta tatcgattta tgtcaagaat actgaaactg gaaacgagga
     7441 tttcagtttg attggacgtg atttcttacc gacggggcac caagactata ttgcaagagt
     7501 ttttggaaca aaagaagaag cgattgatta cttaaaatct atatcttaca tcgcatcagg
     7561 tgttcatggt aacgattggg tttatcaaaa tgaaaaacta ccagaaattg agtcacgttg
     7621 ccgaatttgg aaagtaggag aataaaagga gaacaatatg tttaaagcac taaaaacaat
     7681 caaaaaaatc aaacaacttc agaaagaaat gcacgctttc agccttgcgt ttctagctct
     7741 acaagatatg ggcttgatgc cagaaactga aagaagtaag gcaaaggctc aaacaatgca
     7801 cgatgtaagc cgcgtactca aggacgtctt ggacggcaag tcggtagatg aagcgataaa
     7861 gcgtctaaat agcgaagtga aagctgaaga ggtggggcag aaagatgacc agaattgaac
     7921 ttgaaaaccg tgtatggctt ttggctaatc atgaagaaaa aaacgaattg ctggatcttg
     7981 ggctaacgtc aaagaccaga tatgtgaagc gagttctgga actaggaaag gtgtatgctc
     8041 atgtttgatt atgacagaga tataatgcaa ccgcctgaag aacgagaaga acgtgaccca
     8101 gctgattgga ttttcagcgc tggtcaatgg atctatgtag gagattgtta gcctatgaat
     8161 agagaacaca acgaaaggta gaaggaaatg acgaataatc aaattgtaga agcaaaaggc
     8221 gactttttaa ccaacccaca gctacttaat agcggtatta tcagaaagta tcttgaccca
     8281 caaggaaaag ctagtgatga ggagcttgcc tattttatag ctcaagccaa agcccaaaac
     8341 ctcaatccat ttacaaaaga aatttatttt atcaagtatg gaactcagcc agcccagata
     8401 gtcactgcca aatcagcttt tgaaaagaaa gcagatagtc acccgcaatt tgatggcaaa
     8461 gaggcaggcg taatctatct gatggatggt gaaattaaat actcaaaagg agcatttatt
     8521 cctaaaggtg ctgaaattct tggcggttgg gctaaggtgt accgcaaaga ccgtacttac
     8581 ccaacggaaa cagaagtatc ttttgaggag tacgacaatt ctaaaatacg tgcaagagtt
     8641 aaggaactga cacaacaagg taaagatgtt acttatccag tgatgaactc atacggtaag
     8701 ccaataggtg agaataattg ggatactatg ccttgtgtca tgatacgaaa agtagctcta
     8761 gtgtcagctt accgtgaggc gtttcctgcc gagcttggag caagctatga ggctgacgaa
     8821 attcagctgg ataacacacc taaagacgtc actcctcaag aaagccgtga ggatgtcgta
     8881 gcacgcaaga tggctcagat tgatcaattc aacaaagagc aagaggcaaa tcatgcagat
     8941 cctgaacctg ctaaaactga ggagccaatc cagggcgagt tgctagacgg tgaacttgaa
     9001 tattaggagg acaacatgca agaattacag gttattgatg ataagaaaat caataaaatc
     9061 tatgagatga tcacaacgga tgaacttact agagagtctt ttgaaaaaga cctcatagag
     9121 gctactgaga agtacaagga ctatattcct acagctggaa ctctcaaaga cgacaaggca
     9181 aagcgggctg aatttaacaa gttaattgag tctaaaaatc gtatccgtat tgacactaaa
     9241 aacttactat cagagacagc taacacatgg gatagttatg ctaagtcaat tattgaccca
     9301 tttgcaaccg tagttagtga atttgacaaa ggtatcaagg aaatcgaaga acatcaaaga
     9361 caactaaaaa tagatacggt taagagttac ctagccaaca aatcagctga gtacatgctg
     9421 gatcctcgtc tctttgatga aaaggccctt gagtatgtca aagctagcga ttttatggca
     9481 gatggcgtga cgcttaaaaa agccactatg aagtcacttg atgacatggt cacatttgaa
     9541 tttcagaaac aacaagaatt tgaaaaagcc aagtcagcta tttcagggtt atgtgctgag
     9601 tacggcatga ctgactcacc ttacattaga cagctgaaag acttgactct tgctgaagtc
     9661 tttggacaaa tcaaagctga ttatgagttt gaaaagcaaa aggaagaact cagacaggat
     9721 ctagaacgag cagagcgatc taatcaggag cttttagcag cccaacaaac caaacagcaa
     9781 gaacaggctc caaaatcaac ggaaacccca aattttgacc cagaaacggg caaaatcttg
     9841 gacggtggac aaatcctcca aaatgagcct aacgctctta gaggggctga aaatgaccta
     9901 aaacgatatg cccaaaaaat gactttagag gtgtattttg tagacacagc cgaaaaagac
     9961 cgtttcaagg ctggtctaag tcaactcgga tttgatttta aaaagaacta tcaagtcagg
    10021 ggttatcaac gtatcgatcc attaactcag gctgagctaa atgaacaatg tgggtggtaa
    10081 gtatgacaga aattgaaaaa atttcagaag aattggctga atatggggtg cctgatgagt
    10141 taataggaaa aatagaggac ctattagcga ctctgtatgg cgaaaaaagg aaattggaga
    10201 tagaaaaatc ttgggatatt tctccagagt caatggggag ggtaaccatg gacatcagaa
    10261 aaatatctga cagcgtagcc atctactcgg acggcaagaa attgcaggtt atccacaacc
    10321 taggggatga gtttatccta gattttaagg tgggagagga tagcgtctgg aacctcaatg
    10381 gccaagtcgt agaaattatt gacatgattg agcctgtctt taaagttttc agcttttgct
    10441 caaaatctgg agagggtatg caacgcttaa aacatgctat cgttcacttt gaaaaatttg
    10501 agcagtacat cagagataat caggaagacc tgatgatctg gtggcacaat ccaggagggg
    10561 aatatgatta aaaccgtatt tttttcatgt gattatccac atcatgaggt gattgacgac
    10621 caaataaata gttggcttgc cgaaaatcca ggcattaagt tgattgacat caaatttcaa
    10681 tcaaatgtgt ctgctgtcgc tgacagtgga gtcagtgctg aatattggca tacatccgca
    10741 ttgattattt acaaagttcc ctcagagaac aatataagca gtattaattc aaatggttta
    10801 ggtttcataa tcagctgtga gaaatgtggt agcttatcaa taatcaaggg aaaagatgta
    10861 ggtcaaaatg tatgttatga atgcaaagga gagaaataat gaatgatttt atcaaagaga
    10921 ttgggatggc tatcctatgg atgtttttag ggtatctctt gggagagcgt agcactagag
    10981 ggggacaaat cagatgatca ataacgtcac actggttggg aggcttgtag cgcctcctga
    11041 tctacgaaaa acgcctaata atgtatctag tttacagggc acgcttgcag tcaatcgcaa
    11101 tttcaaaaac gaaaatggag agcgtgaggc tgattttatc aatttccaag cgtggagagg
    11161 tacagctgac atcattgctc agtattgcag caagggctca cttattggga tcattgggcg
    11221 cttacaagtt aggtcttacg agaaagacgg tcagcgtcga tatgtgactg aagtaatcgc
    11281 tgagagtgta gctctgctag agagtcgcaa cagtcagcac ggacaaggca acagtttcca
    11341 aaatgggaat agctcacctt ttaccgatcc taaccccttt gacctcccaa atgacggttt
    11401 gccgttttag gagggacagt tagtggaaat agataagatt ataaaaaaag atgtcttgga
    11461 atttatggaa acaatttctg ataataaaat tgatgcaagt aaaatcaatg tgacagaacg
    11521 tattgctatc atcattgaga aacaaaaaat agaggtcgtt acgactctaa actatgatat
    11581 gagcattagc tttgataaca aagacacggc tcctacacta gatgacaatg gtgacctttt
    11641 tgaaccggtc tacaagtgca aagttaaggc aattcccaaa aatgatgtat ttttcacctc
    11701 attaacacgg gtcaagagca ataccaagac gttacaagag gttaaaaaat tctttgagtt
    11761 cgtaaacgaa aacagagaaa atctctttga gatggcagga tttaaggggg ttcttgaatg
    11821 aaattgaccc tgaacattga gcctaaacct caatcacggc caaggtttgc aagacgtggg
    11881 agttttacca caacttacga agacaaggat atgaaaacat ggcgtaatca ttgccagctg
    11941 ctcattgcta atcagtacat gggtcagcct atccttgagg gagctttgag gacacggctt
    12001 agattttata tcaaacctcc tcagtacatt tctaaagtca agaagaacca acaggccctc
    12061 ctggatgaaa ttataccagt aggcaaaaag cctgacatag ataactacga aaaagcgcta
    12121 tatgacagca tgtcagggat cgtcttccag gacgacggtc agatagcgct acatgatgta
    12181 ggcaagttct acagtctaaa tccacggata gaggttgaga ttgaggtcat gaaatccctg
    12241 agtatttgaa gaaatgagga gcagatggct gactacgcat tatatcaagg tgatgtgttt
    12301 gttacgcttg gaacattagc gcagatcagt agcgagacag gaattactga aaggatgtta
    12361 aagtattaca cttacacatc acaccaaaga cgacacccaa acggtagggc cgttattaaa
    12421 attgaggagg aagataatga gaattaagac ggaaagcgga ggagttggaa gatgatggaa
    12481 gagttaaagc aaaaagttaa tgaagtatac aactggacgg tagaagacgg gaagccgcaa
    12541 cctcccaagc aagatttacc acaagcggtg aaagaccggg cggactattt ttgggaaatg
    12601 gcagaagatg gtatgacgtt tatgggagcg atggaatgca tcttcgctga tgaaaagcct
    12661 atagactatg atttgggagc tactaaggat tggttgccaa aatctaagga gtttgatgat
    12721 tgggttggtt attcgccaag catggctcag gtagttattg cagtttattt gatttatgga
    12781 ggaaactaag atgaatatta aggcattgat taagaagtat gaagaattgt ggaatgaaca
    12841 cagccctttt tatgaacctg taccttatac ttcaatggtt gaactttttt tgaaagagtt
    12901 gaaacaacta gacgaacccc aaaaagtcaa aattccgcag tttgtggcgg attggattga
    12961 ggtttgtaaa gaacatttaa caactagtct atatactgct atgactccaa actttatgaa
    13021 agaaaacaac caaagtttcg atcttatatt atggattaaa aaggcgagca accaagacct
    13081 cttcgctcgc gcatggcttg acggctatga ggtcgaaaaa gagaagcggt atctggtaaa
    13141 gattaaaggg aatattaaag aaaatatgtt ggtttatgga gaacttttgg aaaggtactt
    13201 ctttacaaaa agctttagtt tagacgatgc tatatattcc cacacccgta aagaactaga
    13261 aaatgcaaaa atcggctggg tgtttgattg tccagggatt gagattgagg aggtggagtg
    13321 atgagcctta cgctaaatag cacaattgga gacttagttt tggcaatcgg agaaattatc
    13381 gttggttctg atggtaaaac cactacagcg atactggaga tacctgatca aagcttttac
    13441 ttagagattg agcttaaatt gaaggaggag gtcataaatt gaaacgattc atagctatct
    13501 ggattctgct atctgctgga ttgaatatct ggcagatgga caggattcga gatttggaag
    13561 agaagaagcc gatggttatc tacaaggctg ataacgcagg cgttgagata ttcggtaaag
    13621 tcgtcgagaa aggacggcat ggcaagctat atacgctaac gatacgtgac tatggtgtgt
    13681 tcgtggttac gaaggacgtg tacgataaag tgaaagttgg ggatgaggtg ttactatgac
    13741 agaaactatt aaactaccag actattatga gcctgattgg aaaaatgcaa ggtacgggtc
    13801 gttggaagag cttaaagaat tgttgctctt taagcgtatt gtgaaatggg ataaggactt
    13861 tttgctgctt gaagacggca caaaggtcac tattgaaatg tctgaaagtg attgctgtgc
    13921 ctcagcaggt ggggagttcc aagatgtatc acttgacgct gtgattacta atattgaaat
    13981 tggagaaccg gaagaaatcc ccgaccattg gggaactggt tataaaaaca aagtaactat
    14041 cttccataat cagaaccctg tagctattgc caattgtgaa gcagagcata acggctatta
    14101 ttacagcgta tgctctttag tgattggtga tattcatttt ccagttgtta atgcttagga
    14161 ggatttaaca tgacaccaag atatagagcg tggataaaaa cagaaaaacg tatgtttttt
    14221 tcagatgaca ttcttgctat tgactacgaa aacaaagaaa tagtgacaca acaagtttat
    14281 tttgagaatg gtttaccaga cgatagagat atctattgtt atgattttga cgaaatcgaa
    14341 ctcatgcaat caacaggact caaagacaag aacggcaagg aaatctttga aggcgatatt
    14401 gtacgaacta ctagattttt gggtagagct gacgaaattg gcggtttcta tgagtatgac
    14461 aaggaattta tagggattgt taagcagctt gagggttctt gggtaattga tacgggcagt
    14521 gacgcagtat gtttatggac tgaaattgaa gaaaatgaaa tcatcggcaa catctatgaa
    14581 aataaggagt ttggaggacg caaatgagac cttgtaaata tccatattca ggaagaagaa
    14641 aaaaacaaga aacgccgtcg ccaatatttt ctgcacgacc aatttttgac gaagttccaa
    14701 ttgtagaaga agttaaggtt gagttcggag ttgaagctag tatggggcgc atatatccag
    14761 aaacgttaat acatttagat atttctggat acggaaatag agtgcattca gtacatcgct
    14821 tccccggtat tttactgagt gttggtgagt caatccaact aaagatgctt ttctataaaa
    14881 gacttagaaa ttttactaca gatcgtttct tgacgtttag agaatctgat tggaagttct
    14941 ttatccggga cctggtcaac gaatttaagc attaaaaaaa gccaagacac tctctgcctc
    15001 agctaatagt tctcgcaaag actattatat cacaaaggag acagagagtg aacaaggcta
    15061 aagagctctt gaaagagttg caggatctgg acatggacat ccaaagccgt atagacgaaa
    15121 ttaacgagct tgaggcaggt ttgctctcaa gccctaaatg gtccgaggtt aaagtccaag
    15181 gtggacagac tagaaaagtt gatgatgtct atactcagtt ggtagtgatg aaagaggcta
    15241 tagagcagga tactaaagag gtcattaaca gaaaacttga attaggtaga atgatcaata
    15301 ggcttaaaaa tccaaagtac agggcagtat taagaatgac ttacatcaac aaaggcaccg
    15361 ctgatagcgt ttgttatgat ttgaatatga gtcgtacaac ctactacagg ttaaaaaatg
    15421 aggcggtctt agctttggaa gaagttatct aacctcatag tgagcttatg ggactttttg
    15481 gaacagcacg gttctaaaaa tctgttagaa tggtagtatc aagaattgaa aagagaggtc
    15541 tcagaattgg tagatggtta cctgtaatgt cagggggctg taatggcctt ggaggttcaa
    15601 gtcctcccct ctcctttgag tgtttgtgtc ccagaatgag ttaaatcttc tgggtgggga
    15661 ttcacatatc actcattaac tcctatcact cattaactta aaaattaact taaaaatggt
    15721 tgcggaagcg actggacctc gcatgattgc gtagctaatt atattccgga taagttataa
    15781 gctagagggt ttgattccct cagaggtttt aaagactaca aaaaataaaa aagaagtcaa
    15841 aatttaatac gcacgcaagg tagtagtcgc cttgcaagaa ggtcgcacat cgtgtggctt
    15901 tttttgatta ttcgaagggt ggtgatagaa aattgagtag attgagaata aaacaaaaga
    15961 gatttgcaga tgagtacatc atctcaggta atgcgacgga agcctataag aaagcaggtt
    16021 atcgtgtttc tagtgataga gtggcaggcg ttgaaggaca taagttacta aagaatccta
    16081 agattaaaag ttatatagat gaacggttga aacagcttga ttctgaaaaa atcgcagacc
    16141 agcaagaagt ccttagttat ctaacctcgg taatgcgagg agagacgcaa gaacagactt
    16201 tgataagcat cggagaattg ggtcaaacga ttacggatat tgatgttgga gcaaaagaca
    16261 gaatcaaggc agccgaacta ttaggaaaac gtcataggct ttggacagac aaagtagagg
    16321 cagccgtttc tggaacggtg gtgtttgcaa atgagtcaga cataccagat taaacaaagt
    16381 gatattgtaa tcgacctacc taagacagta ggagctggat acggacagtt ctggcgctca
    16441 agaaatcttt atcgtgttgt aaaaggttcc cgtggttcga agaagtccaa gacaaccgct
    16501 ttgaattatg ttatccgtct tttgaagtat ccctgggcca acttgcttgt tattcgtaga
    16561 tactcgaata ccaacaagca atcaacttat acggatttta aatgggcgtg taatgtgttg
    16621 ggtgtgactc atttgtttaa atttaacgag tctttgcctg aaataactgt aaaagcgact
    16681 ggtcaaaaaa tcctattccg tggtttggat gatgaactca aaatcacatc tatcacggtc
    16741 gatgtcggca gtctttgttg ggcatggttt gaggaagcat atcaaattga gactgaagac
    16801 aagttcagta cagtagttga gtcaatccgt ggtagcctag atgtacctga tttctttaaa
    16861 caaatcacag tcacatttaa cccgtggaat gagaggcact ggctcaaacg tgtgttcttt
    16921 gatgaagaga ctagccgagc tgatacattc gctactacaa ccacttacaa atgcaatgag
    16981 tggcttgatg aagtcgatat taagcgttat gaggacttgt atcacacaaa tccaaggcgt
    17041 gcgagaatcg tttgtgatgg tgaatgggga gttgctgaag gtttaatcta tgagaacgtg
    17101 accgtcaagg atttcgataa ggatgaattg ctacgagatt cagctaataa gttatgtatc
    17161 ggtcttgact ttggttttac tcacgatcca accgctttgt gttgttcgtt gataaatgac
    17221 acgacgaaag agatttatgt ctttgatgag gcgtataaag tcggattgat aaccaaagaa
    17281 gttgcgaaga tgataaaaga caaaggttat catcgctcac aaatcattgc tgatagcgca
    17341 gagtcacggc tgattgaaga gctcaggtca gaacatggca tatctagaat aaaagagagt
    17401 cggaaaggta aggatagtat tatggcaggc gtatcaaaat tgcaaggata cactatttat
    17461 gtgcatccag attgtaaaaa catcatggat gaattttata gttactgcta ccagcgagat
    17521 aaagaaggca actggttgaa taaaccagag gataaaaaca accacttgat ggacgctttg
    17581 cgttacagcc ttcaatgtat cgaaggtggg aaagcaaccg tccgcagacg ttctgattat
    17641 ggtctataga gaggaaagac atgtaccaat atttaaccta tccacgggat ggatatgatg
    17701 agggttcttt gaagaaagac ctgatttaca aattgataac gatacatagc actgaaggct
    17761 cgcatttgaa gaagcttaaa agctactatt tgggtgagca tgctatctta gaacacacga
    17821 gacgcaacgt gaacgcacct aattacaaga cggtagccaa tcatgccaag gatatcgcag
    17881 acacggctac gggctatttt atgggcaatc ctatcaagta taacaatact gctgacggtg
    17941 atatcgatga actacttaca gcctttgatg gtgctgagat tgaccaagta gatgctcaga
    18001 atgctttgaa tatggctatc tatggtcgtg cttacgagta catctatgct aaagagggta
    18061 tggctgagtt ggattcaact agtattgatc cggagaatac tttcatggtc tacgatgata
    18121 gtattgagcg gaagcctttg tttgcggtct attactatga agtaaaagac gatacgaaag
    18181 acactaccaa gcaccaggct gaggtcttta ccgaaaatct gcactatcac atggtgctga
    18241 gaagtacaga ttcaggaaca attcagagcg aggaggcaac acctcacaac cttggtcaaa
    18301 tcccaattat cgaatatcgc aacaatcact ttgcaattgg tgactatgag caacaaatta
    18361 gcttgataga cgcttataat tccttgatgg ggaatcgtgt caatgataag gaacaggctg
    18421 tagagtctat acttgtcttg tatggcacgc agttagcaga cactccagaa gacgctaagg
    18481 tagcaatgaa gattctttct gaagaaggtc ttttggaatt gccgggcgat agtgcaaggg
    18541 ctgagttctt gaagaatacg ctggacgaaa gtgctactga aatcttgcgt acagctctta
    18601 aagaggacat ctacacattt agccatgtgc ctaatttgac tgatgagaat ttcgcaggga
    18661 atacatcagg cgtagccatg gaatttaagc tgatgggcct tgagatgatt actaagacca
    18721 aggaagcgaa ctataagcga ggattgcgtc agcgtattgc gatttttgct cattacttag
    18781 gcatgaagca gattgcttta gagtctcatt caatcgttcc acaattcagt cgtggtttgc
    18841 ctaagaactt gttagaaatc tctcagattg tgaacaattt ggaaggcaaa gtgaccaata
    18901 gacagcttat ttctctcttg ccgtttgtgg aagaccctga cgctgagctg gaagccttgg
    18961 aagaagagaa aaagaagaac atggaagaca tgccgatgtt caacaaagac aacacgaaac
    19021 ccgaagacga ggtagaggat gaagaatcag gagtattggg cgaagaggaa agccaatctg
    19081 atttaccagc agatggacaa ggccgaaaag caggcagatc agttcgataa ggtctatcag
    19141 gaagccaaga cttacttgga taaggaagtc aataagattt ttgataagtt ccaacgtgat
    19201 tatggtctaa gtcaggtaga agctagacaa gtcttgaaga acatgaaaga caagaaaaat
    19261 ctgaatgaac ttcgtaaagt acttgaagcg agaccgaatg acccgaacat ccaaagatta
    19321 ctagctgact tagacagccc agcttattct ttccgtatga agcgcctaga gcgtttgagc
    19381 gacgatttag accgtatgcg tgaatctatc tatcattcag agaagacagg ctcagacgcc
    19441 ttttatagcg acctgatgaa ggatagttac tacaaggcta cctttgacct gcagcagcag
    19501 acaggactag catacggctt ttctgggctt cctgagagcg agattaaaca tctacagtct
    19561 tttagttggg taggagatgg aagtacgtac tcaacaaaca tctggaagaa tacagggaag
    19621 cttacatcaa gcataaaaga tgaattactc ataagcctta tgacaggccg agatacacga
    19681 gaaactgcac aagcaattgc tgagaggttc aatgtaggtc agaacgatgc aagacgtttg
    19741 gttcggacag aatcagcctt ttttcataac caaatggaac tactcagcta tgaagaagca
    19801 gacatagaaa agtatatctt tgcggccgtc ttagacaagc gtacatcacg gatttgtcag
    19861 gagcatgaca atcaggtcta tgatagggac aaggctgtcc ctggcgtcaa ttgtccgcct
    19921 atgcaccctt ggtgtaggtc tactactgtc ggatacgatg aggacgcaga ttacagcaag
    19981 ttgaagcgca gagcaaggaa tccagagaca gggaagaccg agctagtacc tgctgatatg
    20041 acttataaag agtggtatag caagtatgtt gcgaaagacg gggaaaaggt gtataatcaa
    20101 gatacaagag aagccaaggc gaaattttat agcgaacaac tattgtccaa aatttcagga
    20161 gttgagccaa aaattacaag tgatatgcaa cgtatcgcag gagaaaacaa attggcaggt
    20221 cttgaattta ggaagaaaac agttgagtca ttatcacgta aaattattgc agatagccta
    20281 gttgaaaata taagtttgtc aaaagccgtg agtaagatta atgacgcctt aaggtacaca
    20341 actattttcg attccgatac ttttacagaa gagtatttga agatgaaaca gaagcttatc
    20401 gcagaaggtt ataaaattgt aaaagtaaaa aacacttggc cagtagatgg accatacaaa
    20461 ggtgtgaata cagtcgttga aaaagatggt atcaactttg aaatgcagta tcatactcag
    20521 gaaagtttcg acttaaaaaa tggttcatta catgaactct atgagaagta tcgtgatacg
    20581 aatacatctg atctagaacg catgaaatta tttaaggaaa tgcttgattt aagcaatggg
    20641 cttgagattc ctaaaaatat agagagggtg aagtgatatg aaagatatta aatactaccg
    20701 cacaacgacg aacaatgctc aagtacttcg tttgattgat ggtgtcatgc aagtttttga
    20761 cattgaaaaa aagtgggtta atagcatgga ttggtttaat aaaatctttt ttaatgactt
    20821 tacggatttt gaagaaattt cagaaaatga tgcatttact tatattgaca ggatggtagc
    20881 ggcatgattg atattgcctt ggctatcgct aaaaaagcac atgcagggca ggtagataaa
    20941 gcgggtgttg attacataca gcatcctctc tatgtggcca gtcaagtcaa aactgaacaa
    21001 gaaaaagctg tcgctctttt acatgatgtg attgaggata gcgatataac tgctgccgat
    21061 ttattcgcgt ctggcttgtc aaatgaagtt gttacagcgg tacaaatttt gacaaagaaa
    21121 aaaggtcaaa gttatcaaga atatcttggg aaagtaaaat caaataattt agcaagagtt
    21181 gtaaaacttg cagatttgaa acataactca gatttatcac gtttgaaatc tgttaccaat
    21241 acagactacg agcgtgttaa aaaatataaa aatgcaattt attacttaag cacctagaga
    21301 aatctaagtg cttttttcgt gctcagaaag gagaatctga tgaacaagta caaaaagttg
    21361 atagaattga ttgaaaataa cggtcttgag atacaatcta agaaatgtta tgatccacag
    21421 agtgcttggc atggtgagga gttatggatt gttgataaga aaaaacaaaa taaaattttt
    21481 gatttatcag gtaacggtta ctgttttcat gacgctaaag ttgaggaagc cattgaagaa
    21541 gttgagaagt atctattatt gaaaaagatg gatacgtttg atgatttcaa aaaatgggtg
    21601 gaaaagaatg ctaagcctaa aaaatgatgc ttagaaagga gtgaagatat gtttatttgg
    21661 gattgggtat caatcgcttt cgggtggttg gtatttttgt ttttaatgct atttatcata
    21721 gcgtttgtga aaaaagtaat tgaagaaatc aaaaaataat ctaaccgtat ggaattccgt
    21781 acggttttta tattgtccaa actgtaccga tgacattaaa agctgtaccg ttccgtcgcc
    21841 ggacgtaaaa cgagattatc gagtggcgac gtaatcgctg gaggacaatt atgtcagaag
    21901 aaatcaatgc aactgtatct actgaatcaa ctgagactgt cgacactcaa gaaaatgttg
    21961 atacggtgca ggaagaaaag cacgaacgaa ctttcactcg tgctgaaatc ggtaagatgc
    22021 tatctgccga acgctctaaa tgggaagctg agcaagaagc caaggaaaac gaagctaaga
    22081 aacttgccaa gatgaacgct gacgagaaac agaaatatca gttggatcag cgtgagcaag
    22141 aactagctga ccgtgaaaaa gctattgctc gcaaggaatt gaccgcagaa gctaaagcaa
    22201 tgttaagtga acgtgactta cctgttgagt tagtaaatgt agttgatttg acaagcgcag
    22261 agacggtatc tgaatctatt acctctatcc aaaaagcatg ggaagagtca gttcagaagg
    22321 gagtttctga acgtatgaaa ggtagtgcac ctatcaaaaa tgcacaaaca gtccagcaag
    22381 aagtcacgga aaaatggcgt aaagacttct tgtaataaaa gaaaagagga aaaataaatg
    22441 gcatttgaag aattaaacac agcagaatca cgcaagaaac atcttgggat tattgaggat
    22501 gtgcttgcgg taaattcata ttcaacacca cttgtaacat caagcgatgc agtaaccttg
    22561 caaggtcgct cttttacagt agcaactggt aacacaacag agttgaaaga ctacaaacgt
    22621 aacaaagaca acgaatttga tcacgttgaa gttgaagaaa aggtttatac ccttgatgaa
    22681 gaaaaatact ggggtcgttt cgtagatcaa ttggacgaac gtgactctaa tggtcaagtg
    22741 aatatcaatt atgttattgc ccgtcaggct gcagaagtag tcgctccata tcttgatgaa
    22801 ctacgttttg gtgcagcact tggaaatgta agtgacaatg ttgccatggg taaaacagca
    22861 ggagcgaaca acgcttataa tgcggttctt gatgtgtctg agaaacttga tgagcttgga
    22921 attacaaaag aacgcttgct cttcgtcact ccaagtttct acaaagcgat caagtctgaa
    22981 atcgttcgtc taccacatgg tgacgcagat aagaaagtcc ttggaaaagg atatgttggt
    23041 gaattggatg attacacagt ctataaggtt ccttctaaat tcctgaaagg tgttaatgcc
    23101 cttgctactg ctccaggtgt tgttacatct ccagtacaag tagataatac taagtacaac
    23161 gataacattc cagggcgatt tggcgaattg gtagagcaat tgctttacac tggtgcattt
    23221 gttcttgaac acttcaagaa atacatcatc acaattgcag attctaagcc tgctgctaaa
    23281 ccatctactc aaggtaaagt tgtaaaccgt gctaaagcgt ggaaaactgg aacaacctac
    23341 aaagaaggtg acacagttac tcatgccgat aaagtctatg tagcggttaa ggatatttct
    23401 aattcatcaa ccgcaccaga cacagataca actaactgga aagaaaaaac tggtaagaaa
    23461 taggtccgag ttatgaaatt taaaatcaaa caagatttct atgattggga atcaaatgtg
    23521 aaacgactgg caggagagga acttgagatt actgaggagc gctatgctga actggctgac
    23581 aatattgcca gcaacggtgt cgctatctca gatgttcttg aggaaatctt tcctaaacct
    23641 gagttcttag aagaggattg atatgtctat agagttgctg aagaaattaa caggcgaaga
    23701 agatactcag cttctcatgt tgctccaaac aagggctaca aatcttatct tgtcagagac
    23761 taatcgcaca tctttgacac ctgctttaag tcttttaata cctgaggttg ctatcgaact
    23821 ccacaaccgc tcaggagcgg aaggagagca ttctagaacc gagggtggta tagcagtagt
    23881 ctacggagaa aacggcctgt ctacggatct tctacagcga atacgcatgc acaggctagc
    23941 aagggtggca ggtcatgttt ttgaagcaga gtagactgaa accttatcca atgcgacggt
    24001 ttgaaaagac tgtcactgag gaaggtgtcg cgaaagaagg gtatgccaag gaagctgaga
    24061 cagtccgcct tgaattgtgg ccagctagta gcaagctaca atctgaattg tatggcgagc
    24121 gtgtcaatga tattttgaac gcaaatgcca acaagtcagc tactatcaaa gtaaaagatg
    24181 gtgtgtgtat cgatagtcag acggaagtga ctcatagggt tatttctaaa aaggtctaca
    24241 cacatcatca agttttggag ttagagcgtg tcagggctac taggggcaga taggcttata
    24301 gctaaatgta gacgattggc tagtaaaaaa actggcgagg atatcgtctt acgtgcggtg
    24361 cacaatgcta ctataaaggt tgtccaagca gatgcaagaa gactcgcacc agcgagagat
    24421 ggagagctta taactagtat caaaactagg gcaaaaatgg acggagatag ggctataggc
    24481 gaagtttaca ccaaccttaa atacgctcct tatgttgagt ttggaacggg accaatagga
    24541 caagctaacc attcgggtat ctctccagag gtcagcgtaa cttacaagtc taatccttgg
    24601 tatgtgcatg aagaccaaat caatgtagga ccgtaccact ttcaaaagat tggggagttc
    24661 tacaagatgt atggtcaacc tgctcagcct tatctttatc cagctttgag agacaatcaa
    24721 gagcgtgtgt ctaagaatat ttcgaattat gtccgtagaa agataagaga acaaataaaa
    24781 tgattaatat caagcctgtt atttataaag aattgcaaaa ggttgcagat aatgtgaccg
    24841 atacttatcc tagcgattgg gagactttcc cagtcgttat ttttttggaa gaacaaaaca
    24901 agccgggtga ttggtttgat gaccaggaac aaaaatcctc tatccgctac aaggtggata
    24961 tctttgatga taccagcact agtgagttag ctgttaaaat caatcagatt tttgagtctt
    25021 taggtttgcg aagaactgac tgccaagacg tgccagaccc gtctcatttg agacataagg
    25081 tcatgcgttt tgaaggtatc gtcgacttac actcagagct tgtttttcaa tttagaatgg
    25141 agaattaaac atgttagcaa atggaattac gctttcttat ggcgaaacaa aagatagcta
    25201 tactaaactt gttggattga aagaagtacc agagtttggt attgaactcg aaaaagtaga
    25261 aaatactact cttgaagata cggtgaagaa gtacgagttt ggtattgggg acataggaga
    25321 acttgagtac aagttctctt ataataattc aagcgcaact gctccttatc gtgtattgcg
    25381 taaggcagca gacgacaaga agaaactcta ctttgaacaa gcttatccag acggtactaa
    25441 ggtcattttt gaaggccaag tatctgttaa gcttggcggt ggcggtgtca atgccgttat
    25501 cgatttcaca cttaagattg ccttgcagtc taatcttaca tttactgatg gtattggagg
    25561 ttaattaaat ggcgttaaaa tacacaactt ggaaagttac tgacgaaaaa gagttgaagc
    25621 tacgtttgac atctcatcaa gctgcaactg tggaagaaaa aatcggcatg aacttgctga
    25681 agattttcat gcctgaagct ggcgaagagt tcactttacc gcctttgaaa gttatgttgt
    25741 tgttagttca cggagccttg cagcagtatg aacatgggta ttcctttgaa gatgtctacg
    25801 acctatacga tgaatacgtg gataacggcg gagaccaaac aaccttcatg acagaggtgt
    25861 tgatgccact ctttgaagta tcgggtttta ctccacgagg aagcaaggac aagaaaactt
    25921 ccaagaagaa aatgacagta gacaagtaat cttaacggta actcagatta ttgagaggct
    25981 ttatcctatg tttttggaca tcgggggcaa gcctcttgat ttttgggatt tgacggtact
    26041 tgaaatcaga gaaatgattg aaagctataa ccgtgtcaaa atccaagagc gtaaagaaaa
    26101 gattattgac tcatacagac tttcgcagat gatatccaac cacgtttctt tattgttatc
    26161 caaagatgcc aaggcctttg agttctggga atatgcgcct gagttgtttg tagaagaaca
    26221 acaagcagta gaacaggaac gacagaaaca agcacttttg ttgcataagg aacggatgcg
    26281 tgaatttgca gagagacata atcgaaaaag gaaggaggaa gtaaatggca actcttgatg
    26341 aattgaaagt catgattgac gctgagatag cgcctttcag gaagaagatg aaagaagtcg
    26401 agaatcaggt caaaggaaca tctgaccaag tgaaaaatgc tactgccaaa gttcgtgaac
    26461 agtcgagctc aatcggtagt gcgtttggca agctggctaa gttcgctggt tttgcaatcc
    26521 ttggtaagaa attacttgat gttgggatgt attcaacgca gacggctctt gaagtatcag
    26581 cgtctatgaa ccaaatcaag cgacagatgg gcgagagttc gcaatctttc ttaaaatggg
    26641 ttaacgataa tgccaacgct atgaatatgg gtgtgggtga ggctaccaac tacggtgcag
    26701 tctactcaaa cttattttct ggatttatca aagataccaa caagctaagc gcctataccg
    26761 ctaagatgtt gcagacatcg gcagttgttg ctgaaggttc agggcgcacg attacagacg
    26821 ttatggagcg gattcgctca ggtctactag ggaacaccga agcaattgag gacctaggaa
    26881 tcaacgtcaa tgtggctatg attaagtcca ctgaagcttt taagaagttc gcaaacggac
    26941 aaagctggca acaattagac taccaaaccc agcaacaaat ccgccttatg gctattctgg
    27001 aacaggctac agccaagtat gggaatacct tgtctaattc tgtaaatggt cgtatcagcc
    27061 tatttaagtc gctgatgaag gacgcagcat tgaaccttgg taactctatg ttaccgatta
    27121 tcaatgccat tatgcctgtc ttgaactctt ttgctatggt tttgaagaac gttactgcta
    27181 aactcgctga gtttattgct ttgatgttca acaagaaagc aacagtgaaa gatggtgttg
    27241 gtggagcagt tggagacatg ggtaacgcca tgaaggatgc tgcaggcgga gcaggagacc
    27301 ttgctgacgc agtagacgac gctggagatt cagcaggagg acttgctgac aatcttggag
    27361 actcagccaa aaacgctaag aaagctgcta aagagttgct aggtcttttg ggatttgatg
    27421 agattaacat cttgcaaaaa ccaaaagatg acgacgcagg cggttctgga ggcggtggca
    27481 aaggtggtaa aggaaaggga ggcggtggcg gacctttcaa agacatcttg ccagaagtcg
    27541 agttgactga catggacaac aaattcaaga gcatttttga tggtcttgga gataagctca
    27601 aagggttgtt tgacctcttc aagaaaggtt ttgatgcagc atttagacca gaaggtataa
    27661 aacgcattaa gactgcctta gaccaaatag ctaagacaat gggagaaatc gccactgacc
    27721 caagggttgt gaatgccttt aaccgaatgg ctgagaaaat tgcttatgct ttagggcaag
    27781 tgacaggctc aatagctact atcgggctag gtatcggtgt tttccttgcc gaaagtattg
    27841 caaatggcct tggaaggcaa aaagaacgca ttaccagggc gctagtcgct ttgtttgata
    27901 atgttggtaa cctttccgag gcagtaggaa acatagctca ggacttttct agtgctttct
    27961 acgacgtcat tacctcaact ggtgcggttc gtatcggtag cgctattgtg tcaactctgt
    28021 tgagtttgac atctaccatt gttgaagttg gtagtaaatt agcaggaagt ttgtttaaag
    28081 gttttgaaaa agtcgttgtg acaagcgctc ctaaaatttc atcagtcttc caaagtttat
    28141 tagatactgt tgcgcctgta tttgagagca ttgaaaggtc tgttaacaaa tttggcgatg
    28201 gcttaagtcg tgtttatgat gaacatgtag tccctgctat taactctatt gctaatgctt
    28261 ttaatgggct aattgacatt attcagattc tctgggagaa ttcctggcaa ccttttgctg
    28321 agtttttatc aggagtattc ggtgttagta ttgaaggaat ttcagattta ttaggaggtg
    28381 gccttttagc cactttggga ctattggcgg atgctattaa gttagtggca gatggtttca
    28441 ccgttttttc tgactggtgt aaagaaaaca aagaacctat cgtagctttg ataacaactt
    28501 ggcaaacgat taatttctta tcatgggcag aacaagctgg aggacttgca ggagcattca
    28561 gcttgttagg tagtaaggtc tctttgattg ttggaggaat taagaatcta ggtcttgcta
    28621 ttaaagcatt gacatttgat aagttggtca gttttgctga aacaatctat ttgaacacct
    28681 tatatgcaaa agattttgtg gtcaattcag gtaaaacaat tgcacagcta ggaaaaactg
    28741 ctttagaact tggtaaatca gctctagcat ggactgctca tgcagcgaaa atgggattag
    28801 caaccgcggc ggaatttgca cattctgttg cagcaggagt cgctacagct gcaacatggg
    28861 cttttaatgc agcgttagca gttttgacaa gtccaataac atggattatt gcagcaatcg
    28921 cagccttaat tgctatcggt gttttgctct accaaaattg ggacactgtt gttgagtttg
    28981 ctaaaactgc atggcaagga ctatgtgatt ttatcagtgg tatttgtcaa gcgattggcg
    29041 aatttttcag cggtctatgg acgaaactac aagaaatctt tgagccgata ggtcaatggt
    29101 ttggcgagaa attccagcaa gcatgggacg ctattggaaa tatatttggc aacttaggct
    29161 cttggtttgg tggccgttgg aatgattcta aaaacgcact tgccgaagca aatacttggc
    29221 ttggtgataa gttcaagtca ggtcgggata aagtgaactc agcttttgaa aaagttggct
    29281 cttggttcgg tgaccgttgg aatgatatca aagatggagt gaaagaagct gatacatggt
    29341 ttggagagaa atttgagagt gcaaaaaaga aaactcagaa tcctttccaa aaaatcggtt
    29401 cttggtttgg tgatagatgg aaagatatgc aagatgcctt gaaagaaatc cccaactggt
    29461 tcaagaatct gtttaatgat gcaatggata atgctaaaaa catcgttaaa agtggtatcg
    29521 ataaactaaa aagcttcttt aactttgatt ggagcttacc aaaaatcaaa ctccctcact
    29581 ttaatatatc tggtagcttc agcttgatgc ctcctagaat tccatcattc tctgtagatt
    29641 ggtatgcacg aggcggtgta ttcaactctc ctagcatcat tggggtcgga gaagctggtc
    29701 aagaagcggt aatgcctctt gaacggaata caggatggat ttctatcttg gctcaaaaac
    29761 tggctgaaag aatgcctgct aataatgtac ctacaggtta ttcattaccg gctggcgaca
    29821 tcgttatcca aatcgcaggc catgagttcg gacgggtagc tatccaagaa atcaacaagg
    29881 aacatgaacg agcaggtcaa accttgctca agatttagga ggttaaatgg cacaattgac
    29941 aatcaatggg gtggctgtga agcctcccaa atcttttcaa gtcggtattc aagatatcga
    30001 tggagagaca gggcgtaatg ccaatggcga catggtgcgt gaccgtatca cgaccaaacg
    30061 caaactagac tgtgaatggg gtatgatgac tcagggagaa ataagtcagc ttttacatgc
    30121 tgtatcatct gaattttttg aggtatctta tccagacccc atggatggcc aagtcacaaa
    30181 gactttctat gtcggtgcta ggacagctcc tagctatacc tttactgaga agtttaaacc
    30241 ttggtctggc gctaaattta atctggtaga gaggtaagaa aatggacgct ttaactagac
    30301 gacaatttga cagatctatg tttgccaagg aaaggacgct ggctattcgt gttggtgaat
    30361 atgcttcacg ggatatcaaa gaggctagtt ttgagtatgg ctacatcaag ggcgatactt
    30421 ataagcctgg tggaacctgc gctggtagcg gtaaaattac ctttaccagt atcattacca
    30481 cgttcaataa gctggatacc ctgcaccctg agattggtct actggttggg gatacctacc
    30541 agtgggtcaa gatgggggaa tacttcatca acgatattga gattgaccga aaccgaaaca
    30601 ctaccacgct tgaacttatg gacggtatgt ttaagctcaa tcgtgagtac gtgacggact
    30661 tgcatttccc agctgaagta cgagaggtta ttcaggaaat ctgcctgaaa acaggcattg
    30721 agttagcgaa tgactatttc ggaatcagcg cgatgcgtta tcatattgag caagttcctg
    30781 agggcaagaa actttccttt agggatatgc tgagcgctat gactcagatg attgggatgt
    30841 cttgtttctt caacagagaa ggcaagatgg aaatccgtga tttgactgag tccaatatca
    30901 cgatcaacgc tgacagttac ttcttgcatg gcttgaccaa gagtgagatt gagtatcaga
    30961 tagctggtat cacttgtaag acggacaaga agtctctgac ggtcggtatg aagacgggcc
    31021 ggtctttgga actggacaat gtcttcatga cccagagcgc tttaaatgac ttgtattaca
    31081 aactgaaaaa cctaacttac tatccgtata atctcaacta ccaagggcat ttactgcttg
    31141 aggtcgggca gtgggtaacc attcagacca ataagaaaga gacctttaaa gttcctgtgt
    31201 taagtcagag ctttactttt aaaggtggtc tgagagggcg tatcagcgca gatagtaagg
    31261 ctggaaacga tactcagtat tcttacgagg gtacgattac caagcagatt aagcaacaag
    31321 gtggcattga agcgaaaatc caagcgcaga ttgaagcagc agataaagat tttgaccaaa
    31381 aggtcgacaa aatcaaaaaa gactttaacg atcaagtaga actggccaaa gccagagctg
    31441 aagaagtcaa gagagaactg tctgacacta tcaatcagcg ctttaatagc tttgacaacg
    31501 ggccattgaa agaagctaag cgcaaggc
//
LOCUS       DAVLXF010000014        30132 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLXF010000014 DAVLXF010000000
VERSION     DAVLXF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.014"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..84)
                     /locus_tag="VYO12_000598"
     CDS             complement(<1..84)
                     /locus_tag="VYO12_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV1587249.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEM"
     gene            complement(92..533)
                     /locus_tag="VYO12_000599"
                     /pseudo
     CDS             complement(92..533)
                     /locus_tag="VYO12_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
     gene            complement(535..1572)
                     /locus_tag="VYO12_000600"
     CDS             complement(535..1572)
                     /locus_tag="VYO12_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1587250.1"
                     /translation="MNQTINYIKELTAIASPTGFTREISDYLVHTLEKLGYQPVRTAK
                     GGVNVTIKGQNDEQQRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQTVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRVYKEEGIELPVTTHFAFSVFEE
                     VGHGANSNIPTQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            complement(1670..2995)
                     /gene="brnQ"
                     /locus_tag="VYO12_000601"
     CDS             complement(1670..2995)
                     /gene="brnQ"
                     /locus_tag="VYO12_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1587251.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAVAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(3166..3258)
                     /locus_tag="VYO12_000602"
     CDS             complement(3166..3258)
                     /locus_tag="VYO12_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1587252.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(3301..3453)
                     /locus_tag="VYO12_000603"
     CDS             complement(3301..3453)
                     /locus_tag="VYO12_000603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1587253.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDELAALHLEGTHNLPLSQ
                     LADSYD"
     gene            complement(3613..4191)
                     /locus_tag="VYO12_000604"
     CDS             complement(3613..4191)
                     /locus_tag="VYO12_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1587254.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFVAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     DFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMSKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(4245..5645)
                     /gene="pepV"
                     /locus_tag="VYO12_000605"
     CDS             complement(4245..5645)
                     /gene="pepV"
                     /locus_tag="VYO12_000605"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1587255.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFDFAGPAKEYLDITGKILLNDHEGESLKIAHVDEKMGALSMNAGV
                     FRFDETCADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(5658..6263)
                     /locus_tag="VYO12_000606"
     CDS             complement(5658..6263)
                     /locus_tag="VYO12_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1587256.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVCEKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            6434..7234
                     /locus_tag="VYO12_000607"
     CDS             6434..7234
                     /locus_tag="VYO12_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1587257.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKESSSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTYLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSN
                     YIVFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(7412..8245)
                     /locus_tag="VYO12_000608"
     CDS             complement(7412..8245)
                     /locus_tag="VYO12_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1587258.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLQGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEVLDGEYGLEYPKEIQSMRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSKGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQRHNLKLLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(8235..10079)
                     /gene="uvrC"
                     /locus_tag="VYO12_000609"
     CDS             complement(8235..10079)
                     /gene="uvrC"
                     /locus_tag="VYO12_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1587259.1"
                     /translation="MNNLIKPKLELLPTSPGCYIYKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNEHYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKNDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVTAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(10118..10819)
                     /locus_tag="VYO12_000610"
     CDS             complement(10118..10819)
                     /locus_tag="VYO12_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1587260.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETNE"
     gene            complement(10898..12130)
                     /gene="murN"
                     /locus_tag="VYO12_000611"
     CDS             complement(10898..12130)
                     /gene="murN"
                     /locus_tag="VYO12_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1587261.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQNKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(12135..13355)
                     /gene="murM"
                     /locus_tag="VYO12_000612"
     CDS             complement(12135..13355)
                     /gene="murM"
                     /locus_tag="VYO12_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1587262.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(13467..14246)
                     /locus_tag="VYO12_000613"
     CDS             complement(13467..14246)
                     /locus_tag="VYO12_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1587263.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(14315..15976)
                     /locus_tag="VYO12_000614"
     CDS             complement(14315..15976)
                     /locus_tag="VYO12_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1587264.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(16151..18373)
                     /gene="recJ"
                     /locus_tag="VYO12_000615"
     CDS             complement(16151..18373)
                     /gene="recJ"
                     /locus_tag="VYO12_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1587265.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            18650..19408
                     /locus_tag="VYO12_000616"
     CDS             18650..19408
                     /locus_tag="VYO12_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1587266.1"
                     /translation="MVLVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDRMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            19448..20212
                     /locus_tag="VYO12_000617"
     CDS             19448..20212
                     /locus_tag="VYO12_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1587267.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            20225..20902
                     /locus_tag="VYO12_000618"
     CDS             20225..20902
                     /locus_tag="VYO12_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1587268.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGATLLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            20912..21571
                     /locus_tag="VYO12_000619"
     CDS             20912..21571
                     /locus_tag="VYO12_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1587269.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            21829..23019
                     /locus_tag="VYO12_000620"
     CDS             21829..23019
                     /locus_tag="VYO12_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1587270.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVSSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(23340..24221)
                     /locus_tag="VYO12_000621"
     CDS             complement(23340..24221)
                     /locus_tag="VYO12_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1587271.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(24360..25688)
                     /gene="vncS"
                     /locus_tag="VYO12_000622"
     CDS             complement(24360..25688)
                     /gene="vncS"
                     /locus_tag="VYO12_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1587272.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(25685..26341)
                     /gene="vncR"
                     /locus_tag="VYO12_000623"
     CDS             complement(25685..26341)
                     /gene="vncR"
                     /locus_tag="VYO12_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1587273.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(26390..26473)
                     /gene="pep27"
                     /locus_tag="VYO12_000624"
     CDS             complement(26390..26473)
                     /gene="pep27"
                     /locus_tag="VYO12_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1587274.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(26653..28032)
                     /gene="vex3"
                     /locus_tag="VYO12_000625"
     CDS             complement(26653..28032)
                     /gene="vex3"
                     /locus_tag="VYO12_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1587275.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(28084..28731)
                     /gene="vex2"
                     /locus_tag="VYO12_000626"
     CDS             complement(28084..28731)
                     /gene="vex2"
                     /locus_tag="VYO12_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1587276.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(28744..30021)
                     /locus_tag="VYO12_000627"
     CDS             complement(28744..30021)
                     /locus_tag="VYO12_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587277.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         8610 a         6892 c         5618 g         9012 t
ORIGIN      
        1 catctcttca aaagtcgtgc gctgaacacc aacaagacgc ttaaatcgtg catcagttag
       61 ttgtttactt gcttcatcat tcatagaact actataccat attttgtttc gcaggaagtc
      121 taatctcatc cggttttgct tggcgggttt ctgcggtcat tttttcaaaa gggacccacc
      181 agactggccc acagtccttg agaccgtggc cattcatatc acctcttcgt ctagggaaaa
      241 ggtgccagtg gagatgggca tcgccatttc ctagaagttc aatattcatt ttctcagcag
      301 taaagccctt ggcaactgcc tcttggacta aactcatttc ttctagaaaa tggagtcttg
      361 tgtccttttc taaaatggtg caattccgtg acatgttcct tggctagaaa gagactatag
      421 cttgcaaaat actgatggtc tccaatcaca agatagcctg ttcccaactc tttgacaaag
      481 tagggatttt ctcccttctt gatgagctca attctctggc aaataaggca catatcaatc
      541 tactaaattg cttctaagat aggcatcgac cattcgttct gttgcgacca ccgagtcaat
      601 atgagtgcgc tcataagaat gactagactc gatacccgca ccaagaaggg catgcttgac
      661 ctctgcccct gcagacatag ccgctgaagc atccgaacca taaaatggat agatgtccag
      721 cttaaaagga atatcttgct ctttagccaa ggcaaccaag tgttgacgga agtcatagtg
      781 ataaggacct gaagcatcct tgacacagat agatactgtg tactcatctg tctgctggtc
      841 atctcccatt gcccccatat cgacagctag atattccacc acttgagttg gaatattgga
      901 attagcaccg tggcccacct cttcgaaaac tgaaaaagca aaatgagttg ttactggcaa
      961 ttcaattccc tcttccttat aaacacgaag aagattgagc aaaattgctg cgctgacctt
     1021 gtcatccaag tggcgagact tgataaaacc agtctctgtc acaacagttc gcggatcgaa
     1081 actaataaag tctccaacct caatacccaa ggcgcgcgtt tctttttcat tggttacttt
     1141 tgcgtccaaa cgcacttcca tattgtcctg cgtacgttct gcagttcctg catccttgta
     1201 gacatgacaa gaagtctggt ggatgaggat ggtcccagat accgtttgac ctgtgctagc
     1261 cacatgaacg gtacagtttt ctccttcaat catgttccaa ggaaaaccac caatacgatc
     1321 caatttgaga cggccatctg gtttgactgc acgaacaata gcacccagcg tatccacatg
     1381 ggctgtcaca tagcgttgtt gttcatcatt ttgaccttta atggtcacat tgacaccacc
     1441 cttagctgta cgaacaggct ggtaaccaag tttctctaaa gtatggacta gatagtctga
     1501 gatctcacga gtaaagcccg ttggagatgc aatagcggtt agttctttga tatagtttat
     1561 agtctgattc atttcttcac ctctttcgct acctattata acacatttat catcggataa
     1621 aaaaagaaaa tcactcctgt agacttggct acaaaagtga tttaagtgat tattccattt
     1681 caaaaacatc gctttcttgc ttgtttggta gaaccaatga gagcaagatt ccaacaatgg
     1741 ctggcaccaa ccatgggaga gatgccttgg caaaaggaag aacgctaaca agatttgcaa
     1801 gaaactcaac cttaaacgag cttcctagta cgcttgcaat ggcaataact gtaaccacag
     1861 caattgtcaa ctgcatacct ggttttgaaa gggcaacaaa tttgttgaca atgacaatca
     1921 taacgatagc aatcgtgatt gggtacaaga taaccagtac tggaattgag tacttgataa
     1981 tcgcatcaag acccaaattg gcaatagcaa atccaatcaa ggtaaaggct gtcgcataaa
     2041 ccttgtagct gatttgtggg aagcgctcat taaagaactc agctgttgac acaatcaaac
     2101 caacagtcgt tgtgaagcag gttacggtaa ccatagctgc aaggaagagt tgagctgttg
     2161 agccaaagat ttcttgagtg gcttgtgaca agatgtaaac acctggtgtt ccacccttca
     2221 tcgcttcagc tggtactggg aaatgatttc caagaaaacc taaaccgatg taaagagcgc
     2281 tgaaggcaag ggcaacaacg ataccaacaa cccaaatagt tgaaatgtat tctttcttac
     2341 ttgaaaatcc aagttgtttc aaggtttgaa ctgcgattac gctaaaggcc actgaggcaa
     2401 gggcgtccaa ggtattataa ccttctagga aacctgtacc aaaggcagaa gcttgataag
     2461 cagctgaagc agcttgagga cttgttccac catatttgat agctcccaga acgaccaaga
     2521 taacaatcaa aattgcaaag actggcgtta aaatacgtcc aatgcggtct aagatttttg
     2581 atggattaag cgaaatcaaa taggctaccg caaaatacag aaccgtaaat acaatcaagc
     2641 caagtccttt atttgcatcc gacaaaaggg ggctaatccc tacttcgtaa gctgttgtag
     2701 cagtacgtgg ggtagcaaag aatggaccga ttgacaagta aagaactgag aggtaaagag
     2761 tcgcaaacca aggcgctatc ttcgttgaaa tctcgtagat atatccttta ggatttagcg
     2821 ttccaataat aagggtcaag acggcgatac caacgcctga aaagacaaaa cctgcgatgg
     2881 caggaagaaa atgttctcca gatagagcac ctagagaagg cggaaaaatc aagttccccg
     2941 caccaaaaaa tattccaaac aggagcaaac ctgttagggt acctttttta gccataaaaa
     3001 tctccttcat atttataaaa tactgaccta gtataccacg attaggagtc tgaaaaaagc
     3061 atcacgaggc aaatattaaa tgttttcaat tttcttaaaa ataagaatta tctaaaaaca
     3121 aaactctcct acccagtcca caccaagtag gagaaatgca aaattttaaa gttcttcaaa
     3181 ggctaacatg ccaccctgga cattgataac attataacct tgttctaaca ggaattggca
     3241 agcacgcgcc gatctcattc cagatttgca aataatataa tgcaagcgat ctttgtccaa
     3301 ttaatcataa ctatcagcca attgactaag cggtaggttg tgggtacctt ctaaatgaag
     3361 agctgcaagc tcatccactt ctctcacatc cactagagaa agctggtcgt tttggtaaag
     3421 ctgataaaat gcgtcaaagg ctatttcttt cattcttttt cttctaatac tcttcgaaaa
     3481 tctcttcaaa ccacgtcagc gtcgccttac cgtgcgtatg ttaccgactt cgtcagtttt
     3541 atctgcaact tcaaagctgt actttgagca gcctacgact agcttcctag tttgctcttt
     3601 gattttcatt gactataaaa tggttttaat tctttttttc aaatctggca ctacttctgc
     3661 ctcaaaccaa ggatttttgg acatccagat ttgatttcgt ggtgatgggt gaactagcgg
     3721 aaaataggct ggcagatagt ctttatagtg tttcaccctc tccgttacct tcccactgat
     3781 tttctcctgt aaatagtagg cttgggcata ttgcccaatc aagagggtta actgaatatc
     3841 aggcaattcc tgtaagagct gcggatgcca tttttctgca aaatctgtac gaggcggaag
     3901 atcacccgac ttgccatgtc ctggaaagta gaaatccata ggcaaaacag caaaataacc
     3961 tgaattgtaa aaggtatctt catccacacc tagccagtcc cgcaagcggt caccactttt
     4021 atctttccag taaaggcctg cttcttgagt tttaagtccc ggagcctgac cgatgatatt
     4081 gatgcgagca gtttttggcg ctacaaagag aggctcaatg ccacgctctg tatagctggc
     4141 attctgcgaa tccgccatga tagcctgttt gattctttcg atttgagaca tctactttcc
     4201 ctccaaaaag aagtccaagc ataagatctc agacttctat cgttttattt gatcaattcg
     4261 taaatagctt cggcataaat tgctgctgct cggaagagat cgtccaaggc aataaattca
     4321 ttggcttggt gcatagtatc aattgagtct gggaacatag caccataggc aactccgcgc
     4381 tctaacaagc gaccaaaggt tccaccaccg atgacttgtt catgaccttg taggcctgtt
     4441 tgtttttcat agacattcaa caaggtttgc acaagtggat cttccattgg cacatagtga
     4501 ggggtatgac catgttctga tagactgaca gaagcaactg gcaagttttc taggattgac
     4561 ttgatttgtt ctggacttgt tccttttgga tagcggatgt tgagggcaat ggtattatca
     4621 gcacatgttt catcgaagcg gaagacgcct gcattcatag aaagggcacc catcttttca
     4681 tccacatgag caatcttgag actttcaccc tcatggtcgt tcaagagaat tttaccagtg
     4741 atgtcaaggt attctttggc tggaccagca aagtcaaact ggctaaggaa gagggctagg
     4801 taagtcgcac cattgacacc tgaagcaggc atagcaccgt gggctgattt accaatgatc
     4861 gtcaccttgt atttgccagc ttcttcttgg agttctcctc taagtttgtg ttctgcaaca
     4921 aaggcatcta gtttagcttg caagtcagcc aaatcacctg aaacgactgc tgttgctgat
     4981 tctggtacca tattttcacg taaaccacct gtaaagctgt gaagacgggc agcacctgta
     5041 ttttctcctg caaagtggag gtattccgtg atatttcctt tttcaccatt gatgataggg
     5101 aattcagcgt ctggagagaa accgaaatct ggtttggcaa gtcctacgtg ctcaaagtag
     5161 tagtccatat ctgcccagcc tgattcttcg tctgttccaa cgatgaagcg aactttctta
     5221 gaagttggaa gacccaattc tttgatgatt ttcaaaccat agtaacaagc tgttgtagga
     5281 cccttatcgt ccgaagcccc gcgcgcataa aggcgaccat ctttgatagt tggtgtgtaa
     5341 ggatctgtat cccaaccgct accagctgga accacgtcca tatgggcaaa gattccgaga
     5401 acttcttctc catcaccaaa ctcaaaatgt cctgcatagt tatcaacatt tttagttgga
     5461 taaccatcac ggtctgcgat ttcaaggaat ttttccaagg cttttactgg accaggtcca
     5521 aatggatgtt gggcatcagc cttgctatca tcacgttctg agttgatttc caaaaggcta
     5581 aacaagtcag ccaagaggtc ttctttgcgt ttttctactt ctgctgtaaa atcaattgct
     5641 atcatttttt cttcttccta tcttttttcg atgatttcat ctactggcaa gcggtagctt
     5701 ggttccaatt tgtcgtctgt gtaacccact gtgatcaaga gttctgggcg gaaacggtct
     5761 tcgatttcca aaacttcatt aacttttgat ttgtcaaaac caaggataat attggaacca
     5821 attccttggt ctgtaagagc gagaaccaag ttcatagcaa ccaaacctgc attgagtgct
     5881 aggtagtcgc tgacttgttg ttcattgtag cgtgcaaatt cagctggcaa atttttcatg
     5941 aagtattgaa gttgttcttc tgagaaattg ttagcaccac caactcgggc aatcttacga
     6001 gcgcgtttgg caaggtctgt atctgtaaat agggcaatgg ttacaggtgc tgatgatacc
     6061 tgttcaaaat tggaaccata agctaacttt gccagttcag catttttctc acataccacc
     6121 acaaatttcc aaggctggct gttgtgggcg cttggcgcca aggttgcaat ttcgatagcc
     6181 gtacgcacat cttttggatc aacaggctta tcagtgaaat gcttggtcgc atgacgtttt
     6241 ttatttaatt caagaaattt cataatcgat ttccttttct aattctactg cttccattct
     6301 accataattt gaaagagctt gcagggattt ttcatgatta agtttttcta tcacagacat
     6361 aaaagatata tattggttta tcaggaaagt ttctgataaa ctagcaagta ctttacattt
     6421 gaatggagac agtatgaaaa aatttagcct attactagct atcctaccat ttttggttgc
     6481 ctgtgggaat caagctacac ccaaagagtc cagctctcaa aagacaatcg tccttgctac
     6541 agctggcgat gtgccaccat ttgactacga agacaagggc aatctgacag gcttcgatat
     6601 cgaagtccta aaagcagtgg atgaaaaact cagcgattac gagattcaat tccaaagaac
     6661 tgcttgggag agcatcttcc caggacttga ttctggtcac tatcaagcag cagctaataa
     6721 tttgagttac acaaaagagc gcgctgaaaa atacctctac tcgcttccaa tttccaacaa
     6781 tcccctcgtc cttgtcagca acaagaaaaa tcctttgact tatcttgacc agatcgctgg
     6841 taaaacaaca caagaggata ccggaacttc taacgctcaa ttcatcaata actggaatca
     6901 gaaacacact gataatcccg ctacaattga tttttctggt gaggatattg gtaaacgaat
     6961 cctagacctt gctaacggag agtttgattt cctagttttt gacaaggtat ccgttcaaaa
     7021 gattatcaag gaccgtggtt tagacctctc agtcgttgat ttaccttcta cagatagccc
     7081 cagcaactat atcgttttct caagtgacca aaaagagttt aaagagaaat ttgataaagc
     7141 gctcaaagaa ctctatcaag acggaaccct tgaaaaactc agcaatacct atctaggtgg
     7201 ttcttacctc ccagatcaat ctcagttaca ataacactct tcgaaaatct cttcaaacca
     7261 cgtcagcttt atctgcaacc tcaaagctgt gctttgagca acctacggct agcttcctag
     7321 tttgctcttt gattttcatt gagtataaga tatataaaag cgattggact accaatcgtt
     7381 tttagtttgc attggttatt ttaggaaact cttacaaaaa atcaaaaaaa ttcttcacat
     7441 cctctacata cctatgcttt tctattaagc ttgctaatag tttcagattg tgcctctgat
     7501 agttatcttc acgacgtagt tcttcataca tttcgataaa gggaagcccc ttggatttgg
     7561 ccaattctaa ctcagcctcg taatcataag caactttacg aaattggata ttgagtaatt
     7621 ccccatcttc aacctctatc acggcatact gggcacggtg attttttaac gcctcccaat
     7681 taaaataggg catgccaatc gaccctggat tgatgatttg ttgcccttta ctgccgtagc
     7741 gaagcaactg cttgtgaaca tgaccataaa ctgccacgtc cgtttccgca tctagcagtt
     7801 ggtcaaattt ctctgtatca ttctcaacta gcaagtcacc accatagttt ttgtcaggta
     7861 aattatgaga gatagaaaag cgcaatccgt caatttcttt cttttccagc aaaggcaagc
     7921 ttcgtagcca gacaatcgtt gcaggatcca ttcgctccat caaaaactgg gtcatgcgca
     7981 ttgactggat ttctttcgga tattccaaac catattcgcc atccaagacc tcaaggacac
     8041 gatcatccca attgcctcga acacttgctg tgataggaag gtcctttagc agggcgacta
     8101 agtcatttgc gcctggacca ggaagaaaaa tatctccctg aagccaatat tcactggccc
     8161 cttgattttt agcatctgca atcactgctt ctagcgccgt cgcattgcca tgaacatctg
     8221 ataaaattgc gattttatgg ttcattgtgg tttccttccg tttggtaatc tactctttct
     8281 tctgctactt gaggcaaggc ttctccctgc gggttcaact ttctttgcac agcctctgca
     8341 acgactctag gtaccccaac ttcgacaatc tcatccacac tggcttcctt gattttggtc
     8401 aaagacttga aatgcttcat aagattctgc ttgcgtttag gtcccagacc gtcaatccca
     8461 tccaattgag atgagaaaga atttttggag cgcagttggc ggtggaaagt gatagcaaag
     8521 cggtgcacct catcttggat gcgttggagg aggaaaaatt cctgagaatt gcgagacaag
     8581 tccaccacct caagcggatc tccaaagagc aattcatggg tttggtgctt atcattcttt
     8641 tgcagcccag caattggaat atccaagccc agttcctctt ggatgacttg cttagcaata
     8701 ttgacttgac cttgcccccc atcaatcaca atcaaatctg gaggagtcaa agcctcacgc
     8761 tgtactcgac catagcgtct gcgaatgact tctctcatac tagcatagtc gtccggccca
     8821 acaacggttt ttatcttgta cttacggtaa tcttttttac ttggcctgcc gttaacaaag
     8881 accaccatgg ctgaaacagg actggtcccc atgatgttgg agttatcgaa ggactcgatg
     8941 cgaactggag tcggaatttg gagcaaacgt cctagatttt caatagctcc ttgggtcttt
     9001 tcgactgatt tttctagcag attgaacttc tgctctagac taacacgagc attttttatg
     9061 gctagattga ccagctgttt tttctctcca cgttggggtt tgagaatctt ggtatccacc
     9121 aaagccttga cagcttcttc gtcaatatcc tgcggaatca gtacctcatt gggaactagg
     9181 tgagattttt cttgatagaa ttgtcccaca taggtcaaga aatcctcatc tggatcattg
     9241 aagtagggga agagattgac atcgcgctcg atgagcttgc cctgacgaac aaagaaaacc
     9301 tgcacacaca tccagccctt atccacatag tagccaaaga catcgcgatt ttggagatcc
     9361 ttagccatga cccgttgctt ggttcgaagt gttccaatgg cctgaatcag gtcacggtat
     9421 tccgctgccc gttcaaactc catactttgt gctgttactg ccattttact cttgagatca
     9481 tcgatgattt tgtcatcctg acctttcaga aaatcagaca cctcctgggc catggacttg
     9541 aaataggcct cgtcattctt acagatggtg tgggccatac attggccgat atggtagtaa
     9601 aaacagacct tagagggcgg gttggtacat tttcgaaaag ggaaaatcct atctagaagt
     9661 cgcttgattt cattggctgc ccccacatct ggataagggc caaaataaag accaccgtcc
     9721 tttttgacct gacgagtaat gataagccgt ggatagtgct cattggtgat tttgataaaa
     9781 ggataagact tgtcatcctt gagcatgata ttgtacttgg gcttattttc cttgatcagg
     9841 ttgatttcca gcagaagcgc ctcaatatta gactccgtaa caataaattc aaaatccaca
     9901 atttcagaca ccaaggcctc tgttttggta tcatgacttc cacggaaata agacctcacg
     9961 cgattacgca gatttttagc ctttcctaca tagataatgg tgccgttttt atccttgtaa
    10021 atgtagcaac cagggctggt cggcaagagc tctagttttg gtttaatcaa gttgttcata
    10081 gttccattat agcaaaaaag tagggaaaga tagtacccta ctcattagtc tcatatattt
    10141 ttcggaggtt tgcaacatcc tcttcctgga taccataaaa ggaacctgct ggttgcatga
    10201 cagacttctc atctgtatgg tccaagccaa tcgcatgacc taactcatgt tctgccgtat
    10261 ggacaaggcg ttcataggaa tagccatagt atggattgga caaataataa tgattcaacc
    10321 gcaccgttac ggacaagaat tgccctgtta agagattagt ttgactttcc gcctctcctg
    10381 ccacaggagt gcctccgtcg ttcatctccg tagccgtaat atccgcctta ctagactcag
    10441 tcacgagttc aaagttaaaa gcaccagttt gattccagtt ctggattgct tctaaataag
    10501 cctcttgaaa gcgtgaatcc atctgcggat ccaggtaaat acgagcagag gcctgtggcc
    10561 accttccttc agaatgctgg tgactatctg tctggcttaa cttaggcagt tgccctgttt
    10621 tctcccattg gtcaatactt tgttgaccaa tttttactga ccgttctgct tgctttagcg
    10681 ctccttgaaa atccccgttc agataccaga gaagtccgaa agcaagcaca cataagagca
    10741 caactatcca aaccagacgc caaaacaaac gccacacaaa agaaaagaaa gcccctatca
    10801 aacgaaaaag ccagcgcatg tctctccacc tttctagcaa ataaaaatta ttttactaaa
    10861 agaagctaaa agaaatctaa atactgactt tttcatctta acgtcctact atttttttga
    10921 gtaactggat agctttgtat tttaaaggag atggatggta acggaaagta cctgctttgc
    10981 gtacaatata gccattaaaa ttctgtttaa aacgcaaaac accatcactt ccatcaaaaa
    11041 tcccttgaat gcctaggaag ttgtatttag gtattccacg ttttatgctt tccaacataa
    11101 catatttttg aagcagtgca ggggcataga acttattaaa ctcagtgtag gaaccactaa
    11161 agagataagt cgtttcctga ggcatataaa caaataaact cccagctaaa acaatatctt
    11221 cttctccata tttttcaatc aagtctcgcg cttctgcttt tcgaacttca aacgtttcaa
    11281 attgactaga atattctctc agttgatttt gttttttctc agaatgagga tttttactca
    11341 aatcaagtcg caacttgtcc aagttttctt ctagtttatt ttgttcacct tgcaatttgc
    11401 tcatatagtc cgaaaaattt aagcttgcta tgagaaactc cgcttgttct ccaaaagtat
    11461 cataaaaatg ctcataatat tctaaacttt tatcactata ttctctacgt tcagaggttt
    11521 cttttgttat attcttaaaa atcgatagtt cttcacgttt taactttttc aatcgaatgc
    11581 caaaggtttc agcctttttc accaagggtt tacccttttt gctaaaactt ttaagcaaac
    11641 tcttttcagt taattcagtt aaatctttat agtataacca atctggttct ccacctgggt
    11701 aacctgttgt taagccatca aattgataac ctaaatcagt caaaccttga ataatacttt
    11761 ttttctcagc atctattgga ttaccttggc tatcaaaagt ttgataagtt tcataaggtt
    11821 ttacaagcaa ctctaataca ccattttgct tggcatattc ttttaactct gcataaaaaa
    11881 ctggaagagc atcttgttgg gtataaatcg gccccgaatt gagttccata tgcagaccac
    11941 ccagcatggg caagctataa accagagctg caacttgaat ttctccttct tgtttcaaag
    12001 caagataaac aattcgagcc cctctttttt ctagcaaatc ccccatctgg acagattgca
    12061 taaaggaacg agaagaaacc tgatcagaat aagtctgaaa ctcttctttc gtgagtgttg
    12121 ttagtgccat atacttactt tctatgtttt tttcttaatg ttttacggaa atcaagagca
    12181 agtcttaaca gaggatagag aggatgagtg ggcattgtaa attcacccaa gtattcttca
    12241 atcgttggat taaatttttc cttaaaatga taaagtccac cattgagaga gttttcaaca
    12301 ccacctaaat tttgccagac catacctcgc tcaaaggcat agcgagccgt ttcataccat
    12361 gttaaaattg gtgcattgta acgtttaaaa tcatcatcca taccagcata tatattgaca
    12421 gaggtagtac caaattccaa actcaaagta gccgctaaag gaactctcgc ttgacctaca
    12481 tctatatatt cctgcaagaa ggtcaattcc tctaacaaac gttctttttc cttcttctgc
    12541 gcttctactt ttgaagttcg agtcgactca gtaaaggtct cttccaaggc tctatttttc
    12601 gctaactgtt cttctaattc ttgcgaacgt ttagaaacat ccaaggtggc caaggtgata
    12661 taggccttgt ccttaaaatt atctaacaat tttttataat aggcttcatt cctcaaatga
    12721 atctcttttc gcttctcagt ttttttcatc aactccgaaa atgaatctaa tagttccagt
    12781 ccaccatatt gaatctcaag ccctttgttt cgtgctgttc gaatagcctg ttttgttgac
    12841 ttggaaagtt tatcttcttc aaaattttcc ttgtatattt tcgcctgaat acgaggttga
    12901 atggtgtctc ccatttcctc cgtttttcct gaccacctta ctcccatttg ttgcaaacta
    12961 tcaataatag ccagattttc aggaaattct gtcttttcct gattgattaa actttgagat
    13021 aggcaaatac ttgggtcaaa agtcacaaaa accgctctct tactgcgagc ataggactta
    13081 atagactgaa tggcaaaatt caagagttct ttatccccat aatccaatat aggtcctctt
    13141 gggatgtaaa acattttata gcctagcgga agagttctaa tcaaaatact agctgtcgcc
    13201 agtaattttt cttccctgta aacaccaaac ttctcatgtt gccaattaga cttaacttcc
    13261 tcccaagcac tactttgtaa tacattggct aattcatgtt ctttgacaaa ctgatcatat
    13321 tctaatgtgg gaatgccaat ttgataacgg tacatttcct actctctttc ctccagtatt
    13381 ttattactat tctactactt tcgtttgaaa agtctagtga aaacatattt tattttagtt
    13441 ctactccccc tatgctgaag ttcaaactaa gtcagtctct cttctaattt gaaatcaatt
    13501 ggtagggttg ttaaaaaaac ttccaattgt ttttcccagt agtaccactc gtgagttcca
    13561 gcactatggc tataggtcac atcaaaacct agttttttga gatttttcac tgcgagatta
    13621 ttggcttcgt acaagaaatc ctgttcgcca caccacgccc aaagtttggt ctttttatcc
    13681 gattttttag ccagactttc aagagaatag ggactagttg tccagtctct aatctctcca
    13741 aaaacacctc tccagtaggc tggacttccc agattttgac tttcaggaga aaagttttga
    13801 aagctgaggg cacctgaaaa actagctgca tgagaaaaac gatttgtcgt aagagccagt
    13861 ttgaagcagc cgtagcctcc catagaaaga ccagcgataa aggtcttttc acgcttgcta
    13921 gtcatattag ggaagaagcg tttcagaacc tgcggcaatt cctctgctag agccgtgtag
    13981 tagtcaaaac catactgggt atcggtgtac caaccattgc tggtattggg cataacaacg
    14041 atgagattag ttcctcgaag caagcgttct acattggtcc gcttaagcca actattatga
    14101 tttccagaca tcccgtgcaa aaggtacaag acgggaatat cttcacactc tggttcttcc
    14161 actcgattgg catcagggta gaggacattc accccccact ccatatccaa tacttgtgag
    14221 taatactcga ttttcattac tgccatgatt ttctccttct atttttctat aagaaaaagc
    14281 cgaaactcga ctttctctct gtttatttca acgattattt tgcttccatg actactggta
    14341 aaatagctgg acgacgcttg gtttggtcaa agagatactt ggtcaaatta tctcgaacct
    14401 tccctttaag atctgcccag tcaaagtcat ctccttgaag ataatcttct accgtttggt
    14461 taatcaattc tgaactttca cggagaatat cgcgactctt cttgagataa acaaatccac
    14521 gcgtgtgaac acgagcccta gccacaattt tcttctcacg acggttgact gtaatagcca
    14581 cgatgaaaat tccatcctct gacaagacct tacggtcacg aagaacaaca tttccaacat
    14641 caccaatggc attcccatca atcaagatat ctcctgctga aaccgatcca gctggaacaa
    14701 agtctccatt ctcgtaagcc atggtcgtcc cctttttagg aatgaaaatg cgttctggca
    14761 acatcccaac tgccatggca gccttagcgt gagcatccaa ctcacgatac tccccttgga
    14821 cagggaagag gtacttaggt tgcaaaagat tgatcatcag ctgcaaatca cgcacatttc
    14881 cgtgccctga tacatgtaaa ctttgggtaa tcaatttgac aaccccacct gcctgataaa
    14941 tcatattttc cacacgcgca acaaaggctt ctttagcaat agacggagcc gtagcaatat
    15001 agaccaggtc cccatccttg atttctacat aacgatggcg accaatcgac atcttacgaa
    15061 gtccattgat aggttcaccc atacgacctg tctcaagaat aatcaactca tggtcttcaa
    15121 agcgagacat atctttaggc ttaatcaaga gaatttcgtt ggctaaagac aacttcttaa
    15181 gacgaatcgc tgtgcggacg atattttcaa tatcaaatcc tgtcaagacg atacgtcgac
    15241 ctgttttatc cgcagcgtca aaaatctgct gaatacgaga aagattactg gaaacagctg
    15301 caacgatgat acgaccttcc cagtcagcaa tagtttgggt aatttcatcc ctaacttcac
    15361 tttcactagc cacctgaata ttgctgtctg cattggccga atcactgagg agagccagga
    15421 cgccgtcacg accaatctct gccaaacgag caaaatcagt tgcataagat tcactagccg
    15481 tttggtcaaa tttgaagtca cctgtataaa cgatgcttcc ttccgatgtc ttcaagacaa
    15541 ttcccagact ctctggaacg gagtaagtcg tagggaagaa ggaaaccact gtcccaccaa
    15601 aatcaatctc cgtattctca tcaatgacat ggaaatcatt aaatttctta acggcatcat
    15661 ttcctttgac aaagagcttt gccaactcaa tggtcaactc agacccaaat acaggaactt
    15721 tagcctctgc caagagataa ggtagagcac caatggcatc cgcatgcccg tgggtcaaga
    15781 aaaccccagc aatacggtcg ctattttcaa aaaggtaatc catgtttgga atcaccacat
    15841 cgacccctaa ttgttcattt tcaggatatt ttaaccctac attcaaaaca aaaatggact
    15901 ctccaatttc agcaatgtac atattttttc cattctcacg cacaccacca agtgttgtta
    15961 aactgatatt actcattttt cctccaaaat cttagtttat cttgattata gggcaattct
    16021 accctcttat tctaaaagtt ctgatacttt cagccgaatc ttttcctacc attataccat
    16081 ttttttgatg attttcaaaa tgaaggtctg ttttcaaaaa agagttgatt tcccaactct
    16141 ttcctaactt ctactctttt tccatcaaaa aatcataaat ttcttgcacc gtacccagcg
    16201 ccatcatttc ttggtcttta acggtttgtt tgagattttg gtaaatttga ctttctccta
    16261 tctcccgctt tggcgcctct ttattgactg tcatcacacc atcttttatc gtcacaaagc
    16321 ctagttcttc aaatacttga atcatcttga ccagcaagat ttgttgaata ttaagatatg
    16381 cagccaaatc tttcagcttg tagcgaatat caaactctgg gaactggtaa atagtcttgt
    16441 acaatttagc aaactgatct ctagtcccat aacctgtcag ataataggtc ttgtcaatat
    16501 catttttgaa atagacagca gagaaatgct gttcctgaaa aatggtcttc agctgagtaa
    16561 tatcctctgg aatgtttttt acgacaacag cttcactagc cgcaagattt ggcagttctc
    16621 caggaaaatc caagactgga acaccttctg gcaagactgc attttttcca cgaatgttaa
    16681 aaagttgaac accttccact cgcgcatcca ccatcatcaa ctggagggca gtttggccat
    16741 tccattggtt gacagacaat ttaaccgcta actctagatt cttggtttga gaaaactctg
    16801 tcgcccatct gccttgacca aaggctacca cttcaaaact cgcctcaccc ttggaaattt
    16861 tcagctttag atgggcatta cctgccccca tagtacgagc actttcgacc tgaaaattct
    16921 tgatataaaa aataggtttc tgattatcca ttccaaaagg agctaaacgt tcaaaacttt
    16981 tgaccgtttc caagctaagt gcctccaaat ccaactcttc atctaggttt aacttattct
    17041 tgccaccagc atctgcacct ttttcacgaa cataatcttc caaaacctga gataaatctg
    17101 agagttgctc aacttccagc gtcatacccg ctgcacctgc atgacctcca aaggcgatga
    17161 agaggtctcg atggggatcc agagcttcaa aaatatcgac cgcttccaca ctacgagcac
    17221 tgcccttggc acgaccgtct tctatattaa gaacaatgac tgtctgtccc aattcttcca
    17281 ataaacgacc agccacgatt cctagaaccc caggattcca gccttccttg gccaagacct
    17341 gaaccttctt ctcaggatcc accatggtct tggcttcttc atagatagac tgaacgattt
    17401 ccttgcgctc ttcgtttttc tggtgaatca taagggcaat ctcatgcgct tcctcatcat
    17461 caaatccagt caacaaatca atggcaggat tgggatcatc caagcgaccc aaggcattca
    17521 aacgaggagc aatctggaaa ccaaccgttt cttctgttac ttcgttggca gcaatcccag
    17581 ccatgtccag catttcttgc agaccaatgc gctgggtatg acccaacatt tccagaccat
    17641 attgaactaa gatacgattt tcatccgtca gactcaccat atctgcaata gttccaatag
    17701 cgaccaaatc aagcaattcc acttgcactt cttctaacag ggcacaagcc aacttgaaag
    17761 caactccaca accagccaaa tatttaaaag gataatccgc atctggatgt tcaggatgga
    17821 caatagcata agcatctggc agggtttcag gcatggaatg atggtctgtc acaatgacat
    17881 ctactcccat agactgagcc aatgcaatag cctcatgacc agcaacccca ttgtccaccg
    17941 tcacaatcaa ggaaattcct tcttgctcga taaagtattt ataaacacta gcattagggc
    18001 catagccatc ggtaaaacga tttggcaggt aaactcggca ctcagcacca agttgttcca
    18061 aactttcctt cacaatagaa gccgaagtca tgccatccgc atcatagtct ccataaacga
    18121 gaatattttc cccttcttca atagcctgac gaatccgctc cactgccttg tccatatcat
    18181 ggagcagata agcatcatgt aagtcctcca aggaaggttc taaaaacttc ttcagacttt
    18241 cttggtcctg aatccctctc tcaaacaata accgagccac ctcaggaccc agtccagcct
    18301 tcttggctat ctttgtaaaa tccgcatctt ctacctgcag ggcaaactgc cattcataag
    18361 taggtgttat caaaaagaca tccactcttt ctaagaattc tattgcttca ttataacatg
    18421 atttagacgt tttctctatc cagattgctt ttttataaca ttttatagta tattgaaact
    18481 agaatagtac acctctactt ctaaaatatt gttagaaatc gatttgactg tcccgatcga
    18541 tttttcctgt tcttatttca ctatagtgaa ttttttcaga tatgatttga caagaacatt
    18601 cttttattgc agaatcgtgt tataaaatct aacataaagg agttctctca tggttttagt
    18661 agaatttaaa aacgtcgaaa aatattacgg agactaccac gcactccgca acatcaatct
    18721 ccgttttgaa aaaggacaag ttgttgtcct gcttggacct tctggctctg ggaagtccac
    18781 tcttatccgt acgatcaatg gtttagaggc tgttgacaaa ggaagtctcc tagtcaatgg
    18841 gcaccaagtt gctggtgcca gccagaaaga tttggtacct cttcgcaagg aagtcggcat
    18901 ggtttttcaa cattttaacc tttatccaca caaaacagtg ttagaaaacg tgacacttgc
    18961 gcccattaaa gttctaggaa ttgataaaaa agaagctgaa aaaaccgcca aaaaatatct
    19021 ggaatttgta aatatgtggg acaagaaaga ttcctatccc gccatgctat ctggtggaca
    19081 aaaacagcgg atcgccatcg ctcgtggtct tgctatgcat ccggaactcc tcctctttga
    19141 tgaaccaaca tctgctcttg atcctgagac tatcggagat gttctagcag ttatgcagaa
    19201 actggcgcgt gataggatga acatgatcat cgttacccac gaaatgggct ttgctcgaga
    19261 ggttgcggac cgcattatct ttatggccga cggagaagtt ttagtagata cgacagatgt
    19321 cgataacttt tttgacaatc caagcgaacc tcgtgcccaa caattcctca gcaaaattat
    19381 caaccacgaa agtgacaaag tcaaataagg aggcgcctat gaaaaaaaat tctttttatc
    19441 agcattattg attagccttt tcggccttgc tgctgccaaa ccagtccagg ctgatacaag
    19501 tatcgcagac attcaaaaaa gaggcgaact ggttgtcggt gtcaaacaag acgttcccaa
    19561 ttttggttac aaagatccca agaccggtac ttattctggt atcgaaaccg acttggccaa
    19621 gatggtagct gatgaactca aggtcaagat tcgctatgtg ccggttacag cacaaacccg
    19681 cggccccctt ctagacaatg aacaggtcga tatggatatc gcgaccttta ccatcacgga
    19741 cgaacgcaaa aaactctaca actttaccag tccctactac acagacgctt ctggattttt
    19801 ggtcaataaa tctgccaaaa tcaaaaagat tgaggaccta aacggcaaaa ccatcggagt
    19861 cgcccaaggt tctatcaccc aacgcctaat tactgaactg ggtaaaaaga aaggtctgaa
    19921 gtttaaattc gtcgaacttg gttcctaccc agaattgatt acttccctgc acgctcatcg
    19981 tatcgatgcc ttttccgttg accgctctat tctatctggc tacactaata aacggacagc
    20041 actactagat gatagtttca agccatctga ctacggtatt gttaccaaga aatcaaatac
    20101 agagctcaac gactatcttg ataacttggt tactaaatgg agcaaggatg gtagtttgca
    20161 gaaactttat gaccgttaca agctcaaacc atctagtcat accgcagatt aaggaggata
    20221 ccctatgaca gatttatcat cttggacagc ctattttcag gattttggac aatttttcaa
    20281 tggtttcctc tttactcttg ccctagcgat tggatccttt atcctcgcca tggtcttagg
    20341 aatcttcttt ggtgccatgt caaccagtaa acgtccaatt ttacgcattt tagctcgcat
    20401 ctttgtggaa ttttaccaaa atactcctct cttggtgcag tttgttattg ttttttatgg
    20461 cctacctctt atcagtgacc atatcatcat gattccaatt tattggacag ccgttctctg
    20521 cgtgggactc tatcacggcg cttatatcgc tgaggttatt cgttcaggga ttcagtctat
    20581 tcctagtggt cagatggagg ccgccttgtc gcaaggtttt acctatatca gtgccatgcg
    20641 cttgattacc ttgcctcaga ccttccgcat cattctccct ccattgacca accaaattgt
    20701 taacctcatc aagaacacct ctactgtagc tatcatctct ggagtagact tgatgtttgt
    20761 gactaagtct tggtcggctc tcaacggaaa ctatatccca gcctttttag gtgctactct
    20821 tctctacttt gccctatgct tccctgttgc ccagtttggt cgcaagatgg agcaagccaa
    20881 caaaaaagcc tattcacttt aggaggttac tatggaatct attttagaag ttttaacccc
    20941 agataaccta gtctttatct ttaaaggatt tggcttgacc ctctatattt ctctgattgc
    21001 catcatcctc tctactatca tcggtacggt gctagctgtc atgagaaatg gcaaaaatcc
    21061 tgtcttgcga atcatctcca gcatttatat cgaatttgtg cgcaacgttc ccaaccttct
    21121 ctggattttt actatctttt tggtgttcaa aatgaaatcc acaccagcag gtattacagc
    21181 ctttactctc tttacatcag cagccttggc tgagattatt cgaggcggtc tcaatgccgt
    21241 agacaaggga caatacgaag caggaatgtc acaaggcttc acctcagccc aaatcctcta
    21301 ctacatcatt ctcccacaag ccatccgcaa aatgctacca gccatcattt ctcagtttgt
    21361 taccgtgatt aaggatacca gcctcctcta ctctgttatc gccctacaag aactctttgg
    21421 agccagccaa attctcatgg gccgttattt cgaaccagag caggtcttca gtctttgcat
    21481 cctgattgcc ctcatctact tcagctttaa cctagcaatt tctagcctgt ctcatatgct
    21541 agccaaacgt tggcaacaag ctgcagaata atactcttcg aaaatctctt caaaccacgt
    21601 tagctctatc tgcaacctca aaacagtgtt ttgagcaact tgcggctagc ttcctagttt
    21661 gctctttgat tttcattgag tataaacagc cactctccag ttcaattgga gagttttttg
    21721 atgagagtag atatttatag acctcaattt gacaccatac attttctatg ataaaatgta
    21781 acaaatttat ttacaacaaa tcgaaatcta aatgaaatgg aattttctat gaaatcagtc
    21841 aaaggactac tctttatcat agctagtttt atcttgactc ttttgacttg gatgaacact
    21901 tctccccaat tcatgattcc aggactagct ttaacaagcc tatctctgac ttttatccta
    21961 gccactcgtc tcccactact agaaagctgg tttcacagtt tggagaaggt ctacaccgtc
    22021 cacaaattca cagcctttct ctcaatcatc ctactaatct ttcataactt tagtatgggc
    22081 ggtttgtggg gctctcgctt agctgctcaa tttggcaatc ttgccatcta tatctttgcc
    22141 agcatcatcc ttgtcgccta tttaggcaaa tacatccaat acgaagcttg gcgatggatt
    22201 caccgcctgg tttacctagc ctatatttta ggactctttc acatctacat gataatgggc
    22261 aatcgtctcc ttacatttaa tcttctaagt tttcttgtta gtagctatgc ccttttaggc
    22321 ttactagctg gtttttatat catttttcta tatcaaaaga tttccttccc ctatctaggg
    22381 aaaattaccc atctcaaacg cttaaatcac gatactagag aaattcaaat ccatcttagc
    22441 agacctttca actatcaatc aggacaattt gcctttctaa agattttcca agaaggcttt
    22501 gaaagtgctc cgcatccctt ttctatctca ggaggtcatg gtcaaactct ttactttact
    22561 gttaaaaatt caggcgacca taccaagaat atctatgata atcttcaagc cggcagcaaa
    22621 gtaaccctag acagagctta cggacacatg atcatagaag aaggacgaga aaatcaggtt
    22681 tggattgctg gaggtattgg gatcaccccc ttcatctctt acatccgtga acatcctatt
    22741 ttagataaac aggttcactt ctactatagc ttccgtggag atgaaaatgc agtctaccta
    22801 gatttactcc gtaactatgc tcagaaaaat cctaattttg aactccatct aatcgacagt
    22861 acaaaagacg gctatcttaa ttttgaacaa aaagaagtgc ccgaacatgc aaccgtctat
    22921 atgtgtggtc ctatttctat gatgaaggca cttgccaaac agattaagaa acaaaatcca
    22981 aaaacagagc ttatttacga aggattcaag ttcaaataag taatcatact ctcctgggat
    23041 ttcccaggag agttttttat agccaaatga aataagacag gaacgatgga attttggcat
    23101 tcaaatcaat tgataagaat gttttatagt ggagtgaaat aggatacgaa caaattgatt
    23161 aggaaaatca aatgaattta tagaaatctt ttagcagtta tgttgtccta ttctagtttc
    23221 aaaacgctat agaagcagca ttgtgctagt ctagattcag tttactatac taaaacgagt
    23281 agcttgaaat caaaaaaccc accctcacag gcaggtttta tctgtattat tcagctagat
    23341 tatgctttac cttctgaacc gaatacgtcg atacgttctt caaccgatgc ttggatagct
    23401 tttacaccgt cagccaagaa tttacgtggg tcgaagagtt ttttcttgtc gtattctgct
    23461 tcgtttgctt cgtagtcacg agcaaattta cgagttgcgt tagcgaatgc gatttggcat
    23521 tctgtgttaa cgttaacttt ggcaacacca agtttgatag ctgcttggat ttgctcatca
    23581 ggaatacctg atccaccgtg caatacgatt gggaatcctg gaagagcttc tgtcaatttt
    23641 tgcaagtggt caaggtctag accttcccag tttactgggt aagggccgtg gatgttaccg
    23701 ataccagctg ccaagaagtc gataccagtt tcaaccattg ctttagcgtc ttcgattgga
    23761 gccaattcac ctttaccgat gattccgtct tcttcaccac cgatagtacc aacttcagct
    23821 tctactgaga tacctttagc gtgtgctttt tcaacaactt ctttagccaa tttaaggttt
    23881 tcttcaactg gaaggtgtga accgtcaaac atgattgaag tataaccaac ttcgatacac
    23941 tcaagtgcat cttcgtagtg accgtggtca aggtggatag ctactggtac agtgataccc
    24001 attgattcaa caaggttagc gatcaagttg cgagcaactt tgtaaccacc catgtattta
    24061 gcagcaccca ttgaagtttg gatcaaaact ggagcttttt tagcttctgc tgcgcgcaag
    24121 atagcttgag tccactcaag gttgtttgtg ttaaatccac caactgcata accgttgtca
    24181 cgggctgctt ggacaaattt ttctgctgaa acgattgcca ttttatcagg cctcctgtat
    24241 atttttatgg gtcatcccat ttacattgtt cattttatca ctttttgcca aaaaaatcta
    24301 gtttttcccg cagtttcgat tgattttctt ctaactccat ctatgtaaac cctttctccc
    24361 tagtcttgga cgacttttgg aaaatctata aagaaggtta aacgattctc ctccatctcg
    24421 aaacgataag ctaatttttc atgttctaat agactcttaa ccacaaagag ccccatacca
    24481 gaccccttga ccttgcgact agcattgtca gaaaaagact gggctagttt ttcttgttcc
    24541 tctgagctac agctattttc gataaaaagt tctccttctc tttctccaat tcgaactaag
    24601 ccacctggaa cagagtgctt aatggcattg ctgatgagat tagaaagaat caacttcata
    24661 actgatgggt ttagataagc ctgctgatgg gtcaaactat tgtctatctg gagctctctt
    24721 tcctttgcta gcaaggcata atctttgacc agattttgcg tcatctggag gaggtcaatt
    24781 gtttccctat catctcgcaa ttcctgcaca gaagagaggg aaagtatctg cagaacatgg
    24841 tgattgagtt catccacaat ccccaaggca actcccagat actggtctct atccttataa
    24901 cgaccgatat tctctctcat attttcgatt aggattttca aactagccag cggtgttttc
    24961 aattcatgag aagctcctcg taggaattcg accttcatct tctccagctg gagaatggct
    25021 tcattctttt catgcaagtc cgcaataaca gtcaagagat gctggtagag gctattgatt
    25081 tgttccttga gattacctat ctcatcctta gaatccacgc gcaatcgcac ttgggaatcc
    25141 aggtccatca tccgacgggt cacccgcttg atttccaaaa tcggtgcaac aatagtccga
    25201 gcgtagatgt aggccaccaa gagggaaatc agaaaggagg ccagcaaggt atagggaaga
    25261 aactggagac tgatttgctc cgcttccttt tgtaaatcca tggaagctag aaactggaga
    25321 atcatagtac caccgtcttg cgttttcacc tcgcgctcct caataaagag agaggttgtc
    25381 tggcggtctg tgtccagagg aagactgtcc ttgacttcta acttgtcctc ggtcatctca
    25441 cctttgacgg tccccttgat atcactagtc tgggaataca agtctaacac ttgctcgata
    25501 ctctgcctat ctttcccttc tagggactgg gcaatggctg ttgccttttg accaatggtt
    25561 tcctgacgat gactcagata agtcgaagga aaaagaaaat aaatagctaa atgaaggcag
    25621 ataaccagaa cactaaatat cgagaaggta tagataaata tctttgcaaa taaacctgtt
    25681 cgtttcattt tcgctccaat ttataaccaa cattgcgcac agtgaggata caatccaagt
    25741 ctagcttttt ccgcaattcc ttgatataaa catcaataac acggtcaaag ggaacctcat
    25801 ctgtcgcttt ccagacggca tcgataatct gagatcgagt caaggcccgg ccttcatttt
    25861 tcactagata gtccagaatt tccaactctt tggcattgat aggcacttct tgacctgcga
    25921 ggcttgcact gtagctttca aagtccacct tggtatcctt gtaagaaaag attcgtcctg
    25981 tatcgtagta gcgcttgaaa atcgcgtcca ccctcacttt taaaagggag agggagaaag
    26041 gtttttccag atagccatct gccaaagagg caaaggcact catcttgtat tcctcatctt
    26101 gaaaagctgt caacatcaag acaggaacct gactggtttt acgaatctca gctaggactt
    26161 ctaagccgtt gagcttgggc atctggatat ccagtaaaac cagggccacc tcatagctag
    26221 aaaattgctc cagagcttcc tgaccgtccg ctgcctcaat agtttcatag ccacaatccg
    26281 tcaaataatc actgaccccc tcacggatca tctcttcatc ttctacaatt aaaattttca
    26341 tactttaact gctctctatt ttttattttt cttagaataa atacctaccc tattttctat
    26401 tatagtctct tgctggcctt ttgtctgcaa gcaactgacc actagataaa acgttgtgaa
    26461 attcctttct cataaattcc ataactttag tatattatat ttaagcacta aagtacaaag
    26521 aaagcaactg aaagcaatga ttttcaccac tgctttcgga tttattttga attgttaaat
    26581 agccattcct atccactatt cttgaataga aacacaagat gcaatcttta ttctagactc
    26641 attttttcaa atttattcac catccagcaa gagctctttt ggttgttttc taaggagatt
    26701 gcttgaagca agcgccataa cgagaaccac tagaaccaag gcaaggacaa aaatgatgat
    26761 aaagtctgat gtctgaatgg aaatgtctag gctcgacaag gtcttgctaa agccatctac
    26821 ttctgcacca ccaccaaggt tagaggcttg agccgcctta ctagcctgtt tggcaacacc
    26881 tgaagtcaca ttggcaagga cagtgtttcc aattgcacgg gcagtgtaat tagctaggaa
    26941 gtaagcagaa actagagcag ggatagcaat caagatagat tcggtgatga attgacccaa
    27001 gatacttgcc tgcttgaggc cgatagagag gagaattccc acttccttgc gacgggcgtt
    27061 gatccaaagg ctgagcaaga gggcaaggag gagaactgag aagctcaagc taccccagaa
    27121 gaggaggttg gccatcttgt acataccaga gatagattgc tcaagagctg ggtagttaga
    27181 ggagctcttg acgagtgtgt agctcttcca gttgatacca ctgatgccat tcaactcttt
    27241 cataacatca tccaagttct tgtctgctgt tacaaagaag gttgcgtccc cataaatggc
    27301 tgtgtcttct gtgtatccat aaagttttgc agcagtgtga atgtctgtaa tagctgtgtt
    27361 ttcgtaaagt tcttgtgagt aggttactgc tgacttatta tgaccatcaa agagtccctt
    27421 gattgtcact tcaactgttt ccttggctcc tttttcatta tctgcatcgt agatattaga
    27481 gtccagttta accttgtccc ctactttcca gccgtgtttg gctgccaagt ccttgtgcaa
    27541 gaggatttta tccttgtcgt cgttggttaa gtgctctcct tcgactagtt tataagaacc
    27601 agagacaaac ttgtcttctt tagaggagtc attgacacct gtaatcatca agctacttcc
    27661 aaaacgcttg gcacgatcag cagtgagatt cttcttggtt tctggcgttt caatcaggtc
    27721 atatccagtc aaatctccga tagcgttgat acgtttgaca taagactcaa tggccttgtt
    27781 ttcggtgatt tttttgatgt cttcaccctt gatattccca gcaccacgag gcgttccttg
    27841 gttgacgcga cgattgattt gcatggagaa gctattggtg atatttttaa aggtctcctg
    27901 agaagccttg gcagtagctc ccttgattga caagccgacc aaactcaagc tcgccatgag
    27961 gagaataatc aggaagatga caatcgattt gaaaaacttc cttgtaacat aggcaaatgc
    28021 gttgtgtaac atagattccc tttctagatt ttgttttaat cattctatta aaataagctc
    28081 aaattattta ctagtattgc gcgtttcagt cagtttctta tcctttaatt caagtgtaat
    28141 atctgacgct tgtgccactt ctttactgtg agttacgaca atcacacatt tacctgtttt
    28201 ctgggcaagt gatttgagta gttcgacaat atctccagca gttttaggat ccagatttcc
    28261 tgttggctca tcagctagaa taactggagc ttctgagacc aaactgcgag caatggcaac
    28321 acgttgctgt tgaccacctg ataactggag aacattccgc ttgatctggc tttcatccaa
    28381 accaagctca agaagtgtat tcttgcttgc ctttttgttg accaatcgga tattttccag
    28441 cggagaaaga taatctatca agttataatt ttgaaagacc agggaaatat ggtgcatgcg
    28501 atggtaagaa tagcccttct tacgaatatc ctctccttga aaaaggatag aaccttcaac
    28561 aggactatct agaccagcaa gtagggacaa gagtgtggat tttcctgctc ctgactcccc
    28621 aataatactg taaaattttc cgggttcaaa attataattg atctgatata ggactgcttc
    28681 agcagtattc ttataacggt aggtaacatc ttgtaattgt aataaagtca tgatttctcc
    28741 ttcttaacta atagatgata aaatttcttt cggtgatttt ctaaataaga ataggaaaca
    28801 aagggctaca gataagcaac taagcagaac tagaaaaaca taggattctg caaaagataa
    28861 gatgctagtt gataaactgc ttgctttggc tagtgtatct tgtaagcttg cctgatctcc
    28921 acttgctagt agagtttgga gtaggtaagt tgtgattgcg tttcctgcaa caaatgctgg
    28981 aagcaaagct ccaagagata ccaaaactac ctctaaacag aattgtagga agatcgagct
    29041 cttgcctttt ccaagtgcaa gtaaaatccc cacttcatag acccgttctc tcaaccagag
    29101 agacaaaacc agaattaagg ctccagctcc tgctatcaac atcccataaa ggaagatggt
    29161 caggaaggtt tggaaagttg caactgagtc tttgatttgt tcaaaagcct tgttttcctt
    29221 ttcgacttgg tagccttgat tttccaaagc caagttttct acctgcttca tgagtccgtc
    29281 catttcctta ggattttcta catagaagcg tgctgcactg acttgagctt cactattgcc
    29341 caaaagggtt tggctacttt catagtctgt aaagacttga ttttcactga agtcagaaga
    29401 caagcctgtg aatttctctt gttttttacc agaaaagatg ccgataatct caaactctac
    29461 tgtttgtcct tttccagatt cagactgacc agcatccaag ccaatcttgt catgaagcga
    29521 aagaccgttc ttcttagcca attcttcgtg gataaggatt ttcttggaat cccctttttg
    29581 aaggtgtcgc ccttctttta gattgaaagc cgaactggta aaggttacat ccttggatga
    29641 atcctcaaga gccgttaagc taaccaagtt attgtctgca gctgataaat catcacgctc
    29701 cacgctctgc tcgccagtca ctgcttcctt gtcttttagt tttgcgaccg tctcaagttc
    29761 aggagagaca ttttccagcc ccttaatctt gcttacagat gctaggtctg acaacttgaa
    29821 tgtctgacca ttctctatct tcttaataga aaaagatgta ttgagtgatt tataaagatt
    29881 gctttctact gttttgttgg acttcatcag agtcaaacag gctgaaattc cggccaataa
    29941 gaccaataaa atcagaaata aaataaaact tctcagtcgc tttctgctga cataagccca
    30001 agatctttgg attggattca tttgtcacct ccatatttgt aagactatta taaaacccaa
    30061 atatgaaatg tttatgaaat acgaaaaaaa aatatcgagt aggggataat ctctagcccc
    30121 tctcacacca cc
//
LOCUS       DAVLXF010000015        30078 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLXF010000015 DAVLXF010000000
VERSION     DAVLXF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.015"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            511..1674
                     /locus_tag="VYO12_000628"
     CDS             511..1674
                     /locus_tag="VYO12_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1587278.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1671..2348
                     /locus_tag="VYO12_000629"
     CDS             1671..2348
                     /locus_tag="VYO12_000629"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1587279.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2338..3192
                     /locus_tag="VYO12_000630"
     CDS             2338..3192
                     /locus_tag="VYO12_000630"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1587280.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3211..4278
                     /locus_tag="VYO12_000631"
     CDS             3211..4278
                     /locus_tag="VYO12_000631"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1587281.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4288..5454
                     /gene="aroC"
                     /locus_tag="VYO12_000632"
     CDS             4288..5454
                     /gene="aroC"
                     /locus_tag="VYO12_000632"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1587282.1"
                     /translation="MRYLTAGESHGPRLTAIIDGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5464..6567
                     /locus_tag="VYO12_000633"
     CDS             5464..6567
                     /locus_tag="VYO12_000633"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1587283.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6578..6916
                     /locus_tag="VYO12_000634"
     CDS             6578..6916
                     /locus_tag="VYO12_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1587284.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            7008..8291
                     /gene="aroA"
                     /locus_tag="VYO12_000635"
     CDS             7008..8291
                     /gene="aroA"
                     /locus_tag="VYO12_000635"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1587285.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLSPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8284..8760
                     /locus_tag="VYO12_000636"
     CDS             8284..8760
                     /locus_tag="VYO12_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1587286.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGESAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8757..9605
                     /gene="pheA"
                     /locus_tag="VYO12_000637"
     CDS             8757..9605
                     /gene="pheA"
                     /locus_tag="VYO12_000637"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1587287.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9602..10876
                     /gene="psr"
                     /locus_tag="VYO12_000638"
     CDS             9602..10876
                     /gene="psr"
                     /locus_tag="VYO12_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1587288.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     RNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSSPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11155..12000
                     /locus_tag="VYO12_000639"
     CDS             11155..12000
                     /locus_tag="VYO12_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1587289.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11990..13093
                     /locus_tag="VYO12_000640"
     CDS             11990..13093
                     /locus_tag="VYO12_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1587290.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            13095..14081
                     /locus_tag="VYO12_000641"
     CDS             13095..14081
                     /locus_tag="VYO12_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1587291.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            14097..15530
                     /locus_tag="VYO12_000642"
     CDS             14097..15530
                     /locus_tag="VYO12_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1587292.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            15559..16593
                     /locus_tag="VYO12_000643"
     CDS             15559..16593
                     /locus_tag="VYO12_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1587293.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16735..17472
                     /gene="mecA"
                     /locus_tag="VYO12_000644"
     CDS             16735..17472
                     /gene="mecA"
                     /locus_tag="VYO12_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1587294.1"
                     /translation="MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFAEDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17623..18909
                     /locus_tag="VYO12_000645"
     CDS             17623..18909
                     /locus_tag="VYO12_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1587295.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVVHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18911..19780
                     /locus_tag="VYO12_000646"
     CDS             18911..19780
                     /locus_tag="VYO12_000646"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1587296.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            20030..20968
                     /gene="msrB"
                     /locus_tag="VYO12_000647"
     CDS             20030..20968
                     /gene="msrB"
                     /locus_tag="VYO12_000647"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1587297.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21026..22750
                     /locus_tag="VYO12_000648"
     CDS             21026..22750
                     /locus_tag="VYO12_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587298.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISTELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISVKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            22752..24503
                     /locus_tag="VYO12_000649"
     CDS             22752..24503
                     /locus_tag="VYO12_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587299.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     TSDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRF
                     YPISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVI
                     AAAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATS
                     SIDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMD
                     RKGKYYQMQKAAAFSSE"
     gene            24850..26109
                     /locus_tag="VYO12_000650"
     CDS             24850..26109
                     /locus_tag="VYO12_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1587300.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26185..26320
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26359..26859
                     /locus_tag="VYO12_000651"
     CDS             26359..26859
                     /locus_tag="VYO12_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1587301.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26935..27303
                     /gene="rplL"
                     /locus_tag="VYO12_000652"
     CDS             26935..27303
                     /gene="rplL"
                     /locus_tag="VYO12_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1587302.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27490..27660
                     /locus_tag="VYO12_000653"
     CDS             27490..27660
                     /locus_tag="VYO12_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587303.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27660..27908
                     /locus_tag="VYO12_000654"
                     /pseudo
     CDS             27660..27908
                     /locus_tag="VYO12_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27922..28262
                     /locus_tag="VYO12_000655"
                     /pseudo
     CDS             27922..28262
                     /locus_tag="VYO12_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28255..28635
                     /locus_tag="VYO12_000656"
     CDS             28255..28635
                     /locus_tag="VYO12_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1587304.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            28648..28791
                     /locus_tag="VYO12_000657"
     CDS             28648..28791
                     /locus_tag="VYO12_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587305.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            28884..29471
                     /locus_tag="VYO12_000658"
     CDS             28884..29471
                     /locus_tag="VYO12_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1587306.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWSLLVYSLLLF"
     gene            29673..29810
                     /locus_tag="VYO12_000659"
     CDS             29673..29810
                     /locus_tag="VYO12_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV1587307.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT         8955 a         5487 c         6727 g         8909 t
ORIGIN      
        1 tcaatttcaa aaacgttaat tcctataatg tgtgtgatcc aagtattttt gatagaacat
       61 atatttagag ttcgaaatag aatgatgcaa ataggtgaaa ttttaattat tgttgcttct
      121 ataatattat ttattgatag tatattgagt ttgtaacaaa aggcttttct ataaaatcaa
      181 attttccaga tacctattga agcagattta tttcttcatc tttcttctac tacctttgtc
      241 actaaatttg acagacacat attgctgatt caacctctct acctataatc tcacagttaa
      301 cctgaggcta gtttcatagt ttgctatttg attttcgttg ataaaaagaa atgctcctcc
      361 aagatggaag agcatttttt attgtgattc ttacacattt acttcctagt tttgcaggaa
      421 gtctagtata ccatttttta aaagaagagt aaaaaagcaa gggtagtttc ttttttgaga
      481 attgtctaaa agtttgatat aatggtagtt atgaatagaa taagagtcag caaaagggtt
      541 gaaaagaagc ttgctaaggg gctagtttta ctagaagcca gtgatcttga gaatgtcaat
      601 cttaaggatc aggaagtaga ggtgcagggt caggaaggaa actttcttgg gactgcctac
      661 ctttctcagc aaaacaaggg cttgggctgg tttgtcagca aagacaaggt ggccttcaat
      721 caagctttct ttgaaacgtt gtttagaaaa gccaaagaaa agagaaacgc ctactatcaa
      781 gatgatttga caactgcctt tcgtctcttt aatcaagagg gagatggctt tgggggtctg
      841 acagtggacc tttatggcga ctacgccgtc ttttcttggt ataactctta tgtttatcag
      901 attcgtcaga ctatatcaga agcctttaga caggttttcc ctgaggtttt aggagcttat
      961 gagaaaatcc gctttaaggg tttggactat gaatctgccc atgtttatgg tcaagaagca
     1021 cctgactttt tcactgtttt agaaaatggt gtcctgtatc aagtctttat gaatgatggc
     1081 ttgatgacag gaattttcct agaccagcat gaggttcgcg gtagtttagt tgacggcttg
     1141 gctatgggta aatccttact caatatgttt tcctacacag cggctttttc agtagctgcg
     1201 gccatgggag gagctagcca gacaacttct gttgatctag ccaaacgttc acgagaattg
     1261 tctcaagcgc attttcaggc aaatgggctc agcacagacg agcatcgttt tatagtcatg
     1321 gatgtctttg agtatttcaa atatgccaaa cgcaaagact tgacctacga tgtgattgtc
     1381 ctagatccgc ctagctttgc tcggaataaa aaacaaactt tctctgtggc caaggattat
     1441 cacaagttga tttcccagag tcttgagatt ttaaatccgg gagggattat cattgccagt
     1501 accaatgctg ccaatgtttc ccgtcagaaa tttacagaac aaattgataa aggttttgca
     1561 ggaagaagtt accagatttt aaacaaatat ggtcttccag cagattttgc ctataataaa
     1621 aaagatgaaa gtagtaatta cctcaaggtg attagtatga aggttagtaa atgaaattaa
     1681 tcgtttcagt aatgccaaga agtttagagg aggctcaggc tctggatgcc acgaggtact
     1741 tggatgccga catcattgaa tggcgtgccg actatctgcc taaagaagcg attttgcagg
     1801 tagctccagc catttttgaa aaattcgcag gccgtgagtt ggttttcacg ctacgaactc
     1861 gctccgaagg tggagaaatc gacctttctc cagaagagta tatccatcta atcaaggaag
     1921 ttgcgcaact ctatcaacca gactatattg attttgagta ctatagctac aaggatgttt
     1981 ttgaggaaat gctggacttc ccaaatcttg ttttgagtta ccataatttc caagaaacac
     2041 ctgagaacat gatggaaatc ttgtcagagt tgacgatcct aaatccaaaa cttgttaagg
     2101 ttgcggtgat ggctcacacg gagcaggatg tcttagactt gatgaactat acacgaggct
     2161 ttaaaaccct caatcctgaa caggaatatg tgaccatttc tatgggtaag gtgggcaagg
     2221 tctctcgtat cactgcggat gtgactggtt cgagttggtc ttttgccagt ctggatgagg
     2281 ttagtgcccc tgggcagatt tctctagcta gcatgaaaaa aatcagggag attttggatg
     2341 aagcttgatg gctatacacg tttagctgca gttgttgcca atcctattaa gcattctatt
     2401 tctcccttca tccacaatag agcctttgag gcgacagcta ccaacggtgc ttatgtggct
     2461 tgggagattg aagcgagtga cttggcagaa acagtggcca atattcgtcg ctaccagatg
     2521 tttggcatca atctgtccat gccctataag gagcaggtga ttccttattt ggatgagcta
     2581 agcgatgaag cgcgcttgat tggtgcggtt aatacggttg tcaatgagaa tggcaattta
     2641 attggatata atacagatgg caagggattt tttaagtgct tgccttcttt tacaatttca
     2701 ggtaaaaaga tgaccctgct gggtgcaggt ggtgcggcta aatcaatctt ggcacaggct
     2761 attttggatg gcgtcagtca gatttcggtc tttgttcgtt ccgtttctat ggaaaaaaca
     2821 agaccttacc tagacaagtt acaggagcag acaggcttta aagtggattt gtgtgcttta
     2881 gaatatgttt ctgaactgca agcaaggatt gccgagtcgg atttgctagt taatgccacc
     2941 agtgtgggca tggatggcca atcctctcct gttcctgaaa acatagtctt accagaaact
     3001 cttttagttg cagatatcat ataccaaccc tttgaaacac cgtttttgaa atgggctaga
     3061 agtcagggca atccagcagt caatggtctg ggaatgttgc tctatcaagc tgcagaagct
     3121 tttcaactgt ggacaggcaa ggaaatgccg acagaagaga tttggcagtc tttaacagaa
     3181 aaataccaat aaagaaaggg agactctcct atgaaaatca gaatcgatat tcctcatcat
     3241 ccttatgata ttcagattga aaaaggttgt atggcccagg ctggtcagtg gttgcgagaa
     3301 ctctggcaac cgcaaaaggt agtcattgtg acagataacc atgtagcctc tctctatgca
     3361 gagaaggtca agctcagcct agaagatgct ggttttcagg tagctgtttt tgatttctta
     3421 gaaggtgaag aaagaaagaa tttaactact gttcagaaag tctatgaatt tctagtcaag
     3481 caaggtctga ctcgtagcga tggaatcgtt gctcttggtg gtggcgttgt tggggacctg
     3541 gctggctttg tagcctctac ctatatgcgg ggtattcact ttgttcagat tccgactagt
     3601 ttgacagctc aggttgattc ttctatcggt ggaaagacag gcgttaacac accatttgct
     3661 aaaaatatgg tggggacctt tgcccaacca gatggggttc tgattgatcc acttgttctt
     3721 gaaaccctcg gaaaaagaga gttgattgaa gggatgggtg aagttatcaa gtatggcttg
     3781 attgaggatc cagaactgtg ggctctcttg acgggactga atggttctgt tgagagtatt
     3841 ttggaacatg cagagacctt gattgaacat tcttgtcagg tgaagcgcaa gatggtggtt
     3901 gaagatgagt tggacaatgg tattcgtctt tacctcaatt ttggccacac tattggccat
     3961 gccatcgaag cgactgccgg ttatggcaag gtcatgcatg gagaggctgt tgccatggga
     4021 atggtacaga tttccaagat tgctgaggaa aaaggcctca tgccagctgg cattacccaa
     4081 tctatcacag agatgtgtca gaaattcggc ttgcctgttg actatgaaaa ttgggaagtt
     4141 gacaagcttt atcaggctct tactcatgac aagaaagcgc gtggcaacac cttgaaattg
     4201 gtcttggtgc cagagcttgg ttcagcgatc attcacccag tttctctgga agagatgaaa
     4261 gactacttgg taaaataagg agagtgtatg agatatttaa ctgcaggaga atcacacggc
     4321 ccccgtctaa cagctattat tgatggaatt ccagctggac ttccattgac agctgaggat
     4381 atcaatgagg accttagacg ccgtcagggt ggctacggtc gtggtggtcg tatgaagatt
     4441 gagaatgacc aggttgtctt tacttcgggc gttcgccacg ggaagacgac aggggcgcct
     4501 attactatgg atgtcatcaa taaggaccac cagaaatggc tggacatcat gtctgcggag
     4561 gacattgaag accgtcttaa aagcaagcgg aaaattactc atcctcgccc aggtcatgcc
     4621 gatttggttg gggggattaa gtaccgtttt gatgatttgc gaaattcttt ggagcgttca
     4681 tcagctcgtg aaaccaccat gcgggtggca gttggtgcag tagccaaacg cctcttggct
     4741 gagttggata tggagattgc caaccatgtc gtggtctttg gtggcaagga aatcgatgtt
     4801 cctgaaaatc tgacagtcgc tgaaattaag caaagagctg cccagtctga agtttctatt
     4861 gtcaaccaag aacgagaaca ggaaatcaag gactatattg accaaatcaa acgtgatggt
     4921 gataccatcg gtggggttgt ggagacagtc gtcggaggcg ttccagttgg tcttggttcc
     4981 tatgtccaat gggatagaaa attggatgca agattggctc aagctgttgt ctctatcaat
     5041 gcctttaaag gggtggaatt tggtcttggc tttgaggctg gttatcgtaa aggcagccaa
     5101 gttatggatg aaattctctg gtctaaagaa gacggttata ctcgccgtac caataatcta
     5161 ggtggttttg aaggtggtat gactaatggg caacccatcg ttgttcgtgg ggtcatgaaa
     5221 cccattccta ctctttataa acctcttatg agtgtggata tcgaaaccca cgaaccttac
     5281 aaggcaaccg tggagagaag tgatccgact gctcttccag ctgcaggaat ggtcatggaa
     5341 gcagttgtag caacggttct ggcgcaagaa atcctcgaaa aattctcatc agataatctt
     5401 gaggaactaa aagaagcggt agccaaacac cgagactata caaagaacta ttaaggagtt
     5461 cctatggcaa aaacaatcta tatcgcaggt cttgggttga ttggagcctc tatggcactt
     5521 ggtatcaaac gcgatcatcc agattatgaa attttaggtt ataatcgtag tcaagcttcg
     5581 agagatatcg ccttgaaaga aggcatgatt gaccgtgcaa cggatgattt tgctagtttt
     5641 gctcctttgg cagatgtcat tatcctcagc ttgccaatca aacaaactat tgctttcatt
     5701 aaggagttgg ccaatttgga tttgcgagaa ggcgttatta tttcagatgc tggttcgacc
     5761 aagtcaacca ttgtggatgc ggcggagcag tatttggctg gcaagtctgt tcgctttgtc
     5821 ggggcccatc ccatggctgg tagtcacaag acaggggctg cttcggcaga tgtcaatctt
     5881 tttgaaaatg cctattatat ctttacacct tcaagcctga caagtcagga cacgcttaag
     5941 gaaatgaagg atctgctttc aggtcttcat gctcgtttta tcgagattga tgccaaggag
     6001 catgatcgtg tcacttctca gattagccat tttcctcata ttttggcttc tagtctcatg
     6061 gagcagactg cggtctatgc tcaagagcat gagatggcaa ggcgctttgc ggcaggtggt
     6121 tttcgagata tgacccgaat tgcggaaagc gagccaggaa tgtggacctc cattctcttg
     6181 tccaatagcg agatcattct ggatagaatt gaggatttca aggaacgttt ggaagcgatt
     6241 ggtcaggcca ttagtaaggg agatgaagag caaatttgga acttttttaa ccaagcgcgt
     6301 gaacaacgcc agaccatgga aatccataag cgtggtggtg tggatagctc ttacgacctc
     6361 tatgttgacg ttcctgatga agaagatgtc atcctgcgga ttttggaact gctacgtgga
     6421 acttccttgg ttaatatcca catcaacgag gaaaatcgtg aggatattca cggaatccta
     6481 caaatttcat ttaaaaatgc tcaagacttg gaaagagctg agcatctcat aacagaaaat
     6541 actgactata cagtcgtcat caaataagga gaagaacatg tcaaatattt acgatagtgc
     6601 aaacgaactc agtcgcggtc tacgcggatt accagaatac aaggctgtta aagcagctaa
     6661 agatgcgatt gcagcagatg ctgaggcaag caaaatcttt acagattatc ttgcctttca
     6721 agaggaaatt caaaaactag cacagacagg tcaaatgcca gatgcgtcct tccaagctaa
     6781 gatggaaggc tttggtaaac agattcaagg aaatagcctc ttgtcagaat tctttaccaa
     6841 gcaacaacaa ttggccattt acctttctga cattgaaaaa attgtttttg aaccagtttc
     6901 agaattgctg aaataagaaa agaactatcc taaagtcagg aatttttaag gaatttctgg
     6961 ctttttatga taaaataggt aaaagtattg caagtatgag gtccagtatg aaactaaaaa
     7021 caaacattcg ccatttacat ggtagtatcc gcgtcccagg tgacaagtct atcagccacc
     7081 gttccattat ctttggaagt ttggctgagg gtgagaccaa ggtttatgat attctgcgag
     7141 gcgaagacgt tctttcgacc atgcaggttt ttcgtgacct tggtgttgaa attgaggata
     7201 aagatggggt tattaccatt caaggtgtag gcatggctgg cttaaaagcg ccgcaaaatg
     7261 cccttaatat gggaaattct ggcacctcga ttcgcctgat ttcaggtgtc cttgctggtg
     7321 cagatttcga agtagagatg tttggagatg atagtctttc caaacgtcct atggaccgtg
     7381 tgacccttcc actgaaaaaa atgggcgtca gcatttcagg gcaaactgaa cgagacttgc
     7441 ctccccttcg cttaaaaggg acgaaaaacc taagccctat tcattatgag ttgccaattg
     7501 cctctgccca agtcaagtca gccttgatgt ttgcagcctt acaagctaag ggggagtcag
     7561 ttattatcga aaaagaatac acccgtaatc atactgaaga tatgttgcaa caatttggtg
     7621 gtcatttaag tgtggatggt aagaaaatca cagtccaagg gccacaaaaa ttgacaggac
     7681 agaaggtggt cgtaccagga gatatttcca gtgcagcctt ttggttagtc gcaggtttga
     7741 ttgctccaaa ttctcgtcta gtgctgcaga atgtggggat caacgaaact cgcaccggta
     7801 ttattgatgt cattcgtgcc atgggtggaa aattggaaat aactgaaatc gatccagtcg
     7861 ctaaatctgc aaccttgatt gttgagtctt ctgacttgaa aggaacagag atttgtggcg
     7921 ctttgattcc acgtttgatt gatgaattgc ctattattgc cctacttgcg acccaagccc
     7981 aaggtgtaac agttattaaa gatgctgagg agctcaaggt caaggaaaca gaccgtattc
     8041 aggttgtggc agacgcctta aatagtatgg gagcagatat tactcctacg gcagatggga
     8101 tgattatcaa aggaaaatca gctcttcacg gtgctagagt caatacgttt ggtgaccacc
     8161 gtatcggcat gatgacagct atcgcagccc tattggttgc agatggagag gtggagcttg
     8221 accgtgcaga agccatcaat accagctatc ctagtttctt tgatgatttg gagagcttga
     8281 ttcatggcta aggtgttatt agggtttatg ggggctggaa aatcaactat tgcaagaggc
     8341 ttggacccta attaccttga tatggatgct ctgattgaga agcgcctagg tatgtccatt
     8401 gcgaattttt tcgctgaaaa gggagaatca gcctttcgtc aggtagaatc agaagtccta
     8461 gctgatttac tacaaacaga ccaagtcgtg tcaactggag gaggagtggt tatttctcag
     8521 agaaatcgtg acttactcaa gactaataca gataacatct acctgaaagc agattttgaa
     8581 accctctacc tacgtatcgt agctgataag gacaatcagc gaccgctttt tctaaataat
     8641 agcaaggaag aactagtagc tatttttcaa gaaagacagg cttggtatga ggaagtggct
     8701 agtcgggttt tggatgtgac caagctaagc ccagaggaaa ttatagagga actaagatga
     8761 aaattgctta tctaggtccc aaaggatcat tttcacacca cgttgtgcag acagcttttc
     8821 ctcatgagga attgcaggct tttgccaaca ttacagatgt tatcaaggct tatgagcaag
     8881 gattagtgga ctattctgtg gtgccagttg aaaattctat tgagggtagt gttcatgaaa
     8941 ccttggacta tctttttcat caggctcaca tccaagcagt agcagaaatc gttcagccta
     9001 ttcatcagca gttgatggtg gttccaggcc atactaagat tgaaaagatt ttttcacatc
     9061 cacaggcctt ggctcaagga aagaaattca tcgatgaaca atatccagag gctcaaatcg
     9121 aggtaacagc tagtacagct tatgcggccc gttttatttc cgaacatcca gaccagccct
     9181 ttgcagcagt tgcacctaga agttctgctg aagaatatgg attggaactg attgccgagg
     9241 atattcagga aatggaagcc aatttcacac gtttctgggt tctaggagct gaaaagccta
     9301 gtattccctt gcaagcacaa actgaaaaga tgagtttggc cttgacatta cctgacaacc
     9361 ttccaggtgc actttataag gccctgtcga cctttgcttg gcgaggaatt gacttgacaa
     9421 aaattgaaag tcgtccactc aagacagcac tgggtgaata ctttttcatt atcgatgtgg
     9481 attataccga taaggacttg gtccactttg cccaaaaaga attagaagcg attggaatcc
     9541 agtataaaat tctgggtgcc tatcctattt atccaatatc agaccatgga aaggagagaa
     9601 gatgagtaaa gaaaatccct taagtcatca tgagcagttg cgttatgatt atttgctaaa
     9661 aaatattcac tacctcaatg agagagaaaa aaatgagttt gcctatttgc aagaaaagct
     9721 aactcttgct aggagaaata gtagctctag cttggaacaa gaaagagaag agcaggttga
     9781 cttaccaagt tatgcgaacc ggagtcgctc acaatccaaa tcacaagcac tctcttcccc
     9841 tccaaaaaag aaaagacgga agctccgtct taaacgaatt tttatggtga ttttttcact
     9901 tttggtctgt gtggctttgg ccatggtatt catgtttttg cgtggttacc aagatgctag
     9961 cgcaaagaaa actgctgatg cccgggcagc tcaagtagaa gtctttaatg gtcaggacac
    10021 tagagatgga gttaatattt taatcatggg tactgatggt cgaatcggcc agaacagtgt
    10081 tgagacacga actgactcta ttatggtatt aaatgtcggg ggctcagata agaaaatgaa
    10141 gctagtcagt ttcatgcgtg ataatttggt ctatatagat ggttatagtc aagtgattaa
    10201 cggtagaaaa cagacagata acaagttaaa cgtagcctac gagttaggag aacaagaggg
    10261 gcaaaaaggg gcagaaatgg ttcgccaagt cttgaaagat aattttgact tggacattaa
    10321 gtactacgcc ttggttgatt tccaggcctt tgccacagcg attgacacac ttttccctga
    10381 tggggtgacg attgatgctc aattttcaac attgaatggg cgtcccctaa cagaagctac
    10441 agtcggagat gatttatacg ctaccgagac tgagtctcca acccaaacca ttaaagtcgg
    10501 aaaacagcag atgaatggct cgaccctgct caattatgct cgtttccgtg atgatgatga
    10561 ggcggattac ggccgtacaa aaagacagca acaagtttta acagcaattt tagagcaaat
    10621 taaagatccc actaaacttt tcactggttc tgaagctctt ggaaaggttt ttgctatgac
    10681 ctcaaccaac gtaccctata ctttcctcct aacaaacggt ttatctgttt tggatggagc
    10741 aaaaaatggt attgaaaaat tgacgattcc agaactgggt gactgggtag atgcctatga
    10801 tgtttatgga ggcttgggct tgctggttga tcaaaacaaa tatcaaacca agctcgctca
    10861 aatgggcttg agataagata atactcaatg aaaatcaaag tgcaaactag gaagctagcc
    10921 gcaggctgct aaaaacacag ttttgaggtt atagataaga ctgacgaagt cagttaccta
    10981 tacctacggc aaggtgaagc tgacgtgctt tgaagagatt ttcgaagagt ataagacaga
    11041 aaaatcaaag tttgatagct atttggatag cagtcaggct ttgatttttt acagtctgca
    11101 atagattttc accccttgtt tgtggtataa tgaaacgata cgatagaaag aataatgaac
    11161 aatatgactg atttaaaagc aattcaggct cgtagtctgg agatggctga atattttgtg
    11221 gccttttgta aagaacatga tttgctctgt tatctctgtg gcggaggcgc tattggtacc
    11281 cttcgaaaca agggatttat tccttgggac gacgacctag acttttttat gcctcgtaaa
    11341 gattatgaga aattagcaga attatggcct cgttatgcag atgaacgtta tttcttgtca
    11401 aagagttaca aggattttgt tgatcgtaat ctttttatta ccattcgtga caaggaaacc
    11461 acctgtatca agccttatca gcaggatttg gatttgccac atggtctggc cttggatgtt
    11521 ttgcctttgg attattatcc gaaagatcca gctgagcgga aaaaacaggt tcgttgggcc
    11581 ttgatttatt cactcttttg tgcgcaaact attccagaaa agcatggtgc tctcatgaaa
    11641 tggggaagtc gcattttact gggtttgact ccaaaatctc tccgttatcg catctggaaa
    11701 aaagctgaga aagaaatgac taagtatgat ttggctgatt gtgatggcat tacagaatta
    11761 tgctcaggtc ctggctacat gagaaacaag tacccaatca catcttttga agacaatctt
    11821 ttcttgccat ttgagggaac agagatgcct attccaatcg gctatgatgt ctatctcaga
    11881 actgcttttg gggattatat gacgcctcca ccagcagaca agcaggtacc gcatcatgat
    11941 gctgtcatcg ctgatatgga taagtcttat acagaataca agggagaata tggtggctaa
    12001 gaaaaaaatc ttatttttta tgtggtcttt ttctcttgga ggtggtgcag agaagattct
    12061 atcaaccatt gtttcaaatc tggatccaga aaagtatgat attgatattc ttgaaatgga
    12121 gcactttgac aagggatatg aatctgttcc aaagcatgta cgcattttaa aatcccttca
    12181 agattatcgc caaaccagat ggttacgagc ttttttgtgg agaatgagaa tttattttcc
    12241 aagactggct cgtcgtttgc ttgtaaaaga tgattatgat gttgaagttt cttttaccat
    12301 tatgaatcca ccactgttgt tctctaaaag aagagaagtc aagaagatat cttggattca
    12361 tggaagtatt gaagaacttc ttaaggatag ctctaaaaga gaatcacata gaagccagtt
    12421 ggatgctgcg aatacaattg tagggatttc aaaaaagacc agcaattcta tcaaggaagt
    12481 ttatccagat tatgcttcta aattacagac aatctacaat ggatatgatt ttcagactat
    12541 tctagaaaaa tctcaagaga agatcgatat cgagattgct cctcaaagta tctgtactat
    12601 cggacggatt gaggaaaata agggttctga ccgtgtagtg gaagtgatac gattattaca
    12661 ccaagaggga aaaaactatc atctctattt tatcggggct ggtgatatgg aagaggaact
    12721 gaaaaaacga gtcaaagagt atgggattga ggactatgta catttccttg gttatcaaaa
    12781 aaatccttat cagtatttat ctcagacgaa agttctcttg tctatgtcta aacaagaagg
    12841 ttttcctgga gtgtatgtgg aggccttgag tctgggactc ccttttatct ctacggacgt
    12901 tggaggggct gaggaattat cccaagaagg acgatttgga caaatcattg agagcaatca
    12961 agaggcagct caggcgatta ctaattacat gacttctgcc tcaaactttg atgtcgatga
    13021 ggctagccaa ttcattcaac aatttacaat tacaaaacaa atcgaacaag tagaaaaact
    13081 attagaggag tagcatggaa actgcattaa ttagtgtgat tgtgccagtc tataatgtgg
    13141 cgcagtacct agaaaaatcg atagcttcca ttcagaagca gatctatcaa aatctggaaa
    13201 ttattcttgt tgatgatggt gcaacagatg aaagtggtcg cttgtgtgac tcaatcgctg
    13261 aacaagatga cagggtgtca gtgcttcata aaaagaacga aggattgtcg caagcacgaa
    13321 atgatgggat gaagcaggct cacggggatt atctgatttt tattgactca gatgattata
    13381 tccatccaga aatgattcag agcttatatg agcaattagt tcaagaagat gcggatgttt
    13441 caagctgtgg tgtcatgaat gtctatgcta atgatgaaag cccacagtca gccaatcagg
    13501 atgactattt tgtctgtgat tctcaaacat ttctaaagga atacctcata ggtgaaaaaa
    13561 tacctgggac gatttgcaat aagctaatca agagacagat tgcaactgcc ctatcctttc
    13621 ctaaggggtt gatttacgaa gatgcctatt accattttga tttaatcaag ttggccaaga
    13681 agtatgtggt taatactaaa ccctattatt actatttcca tagaggggat agtattacga
    13741 ccaaacccta tgcagagaag gatttagcct atattgatat ctaccaaaag ttttataatg
    13801 aagttgtgaa aaactatcct gacttgaaag aggtcgcttt tttcagattg gcctatgccc
    13861 acttctttat tctggataag atgttgctag atgatcagta taaacagttt gaagcctatt
    13921 ctcagattca tcgtttttta aaaggccatg cctttgctat ttctaggaat ccaattttcc
    13981 gtaaggggag aagaattagt gctttggccc tattcataaa tatttcctta tatcgattct
    14041 tattactgaa aaatattgaa aaatctaaaa aattacatta gaacgggggt gaccaagtga
    14101 ttgatgggaa acgattatta tttagtttga ccatagtcag ttatgccttg acgctagtaa
    14161 gtggaattgt gtatctgttt aataataata atgttagctt actttctact ttattgttct
    14221 tgttggttag tagcttaatt gcttgttgga atgatatcaa gtattactta atccatttta
    14281 ttttctattt aaccattttt gtatttctgg tatcaagacc gaccattgat tattttaggg
    14341 atggtgcttt ggatacctat catccaatag cctatcgttt tgcctttata gttgtcatgg
    14401 tttcgattct gggcttgacc acaggaggca ttctggctcg ttacttcata gctaggaaga
    14461 aaataaaagt agcaaatata ggaaattctc taaaagaggt ttatatcaag cggttacgct
    14521 ttgtatcact aggagttttt cttctaacct atcctttcta tttcattcgg ttatttgaac
    14581 ggctcttgta tcgtttgcag acttcctact atgcctacta tgcaaatttt gaaagtaaac
    14641 tgccttattt tacctacatt ttgtctacct ttacggtcta tgcaatgtgt atgtatctgg
    14701 caaccaagcc aaagaaattg caggccacag cagtgcttgt ctcctttatt gcagctaata
    14761 ctattcattt ggcaattggg acacgaaatc cctttatttt aagtatttta tttgcttttg
    14821 tttattactt tatgcgggag caaactgaaa aaggaaaatg gattgggttt aaagaaaagt
    14881 tagcgatttt tgtaggttct cctattctca tgttagcgat gggagtactc aattatgtac
    14941 gggataatgt ccaagtttcc catacaggtt tctgggatat cttacttgac tttatctata
    15001 aacaagggac tagttttggt gttctggctc gaggttttct atttaacagt agcctccctt
    15061 accgagattt ccgtaatttt acttttggtc ctgttcttga ttattttgca agggggagtt
    15121 tgggagccat tttcggagga aaagcctttg aacatacaac caatagtgtg gaactagcta
    15181 ttgatagtaa tagttatgcc cacaatctat cctatcttgt cttgaacaag gaatacctga
    15241 aagggcatgg tatcggaagt agttatatca tggagttgta taccgactat ggtatgattg
    15301 gagtctttct gcttagtttc ttactcggcg tattatttat agccatgctg caagtagcct
    15361 atcgctcaag gacaatccta tttgctttat ccctactcat cttgaataat ctattcttta
    15421 tgccaagaag cagcttttca gaaagtttct tcaatttatt tacaatgcaa ttctggggaa
    15481 ttgttcttgt gattatattt atagcaaaaa tgcttacaaa ggaaaactag tatctactaa
    15541 acaaaggaga aaaaaatcat gtttgaacat tattcagtag ctgatttgtt tgcaaatctt
    15601 tataaaaagc gaaaagcaaa tattttagct ctcatcgctt tatttgctct cattgctgta
    15661 ccatttacaa ttaaagcagt taggaataaa aacactgtca aagatacaac aagttattca
    15721 acttatctta tctataaaat cactcctcca aaagagtcgg ccaaaacgat tttgaatcat
    15781 caaattggtg gttatagtga tttttatggg aaattgattg atggtaattt gaatggagct
    15841 tatcttttca atgatgtaga acccagtgag ttgaaaaaaa ttgccagtga attagacacg
    15901 acagaaacaa ccttgaaaaa ttcaacgaat gactattggt ggaaaaaatt gaccgtctac
    15961 tatatgattg acgatgcagg ggttggtgtg aaaattttga catcaagtaa agatgccaat
    16021 gacttgttag agaaaaaaat tgatgggttg attgagaaat ttaaacatgc ctatgcaaat
    16081 gtgaaaattg aaaaactgga aaccatcaac tctaaagaat tgaacgcaaa tggtgaaaca
    16141 gcgcttggct taaatgtgaa aaatctgatt cttcgtttag ttgttattgg agtggtttgt
    16201 gtgattttgg ttgtgatggg aaatgtgtta gtttatctct ttaatccaac aatcaataga
    16261 gtaggtgatt tttctcagta tcaaattgat tttgtaacag agattacaac aattgctaac
    16321 ctagcagatg ttttgtcata caaaaatact ggacaggaat tgaccatcgt tagctcaaat
    16381 aaagctatcc tagataaatt gaaacagagt caagaagctt taaaaggaat gcattttgta
    16441 gatttacagg atgtatcatc tcttttggaa agagatacag tccttcttgt tgaagagtac
    16501 ggagtgactc gttataagaa atttgagcaa agtcttcaaa ttctcagaaa cttaaatcgt
    16561 tctatcctcg gtgtagcaac ctttaaatta taagtattct gatgtataag gtcttcaaag
    16621 tcggctaaga gccgattttg aaggcttttt ctatctgtct gcagaaattt tcttttgata
    16681 aaatcgtaaa aatctagtat aatagataga actgaaagta tgaggttact agcaatgaaa
    16741 atgaaacaaa ttagtgatac aactttaaaa atcacgatat ctttagagga tttgatggat
    16801 cgtggaatgg agattgctga ctttctcgtt cctcaagaaa aaacagaaga gttcttttat
    16861 gctatcttgg atgagctaga gatgcctgat agctttctgg atacaggcat gttgagcttc
    16921 cgtgtgactc caaaacctga taaggtagat gtctttgtga ccaagtcaaa gattgaccaa
    16981 aatctagatt ttgaagactt atcggatttg ccagatatgg aagaattggc tcaaatgtct
    17041 ccagatgaat ttatcaaaac cctggaaaaa agcatcgcag acaaaaccaa ggatgatatc
    17101 gaagccattc aatctcttga gcaagttgaa gccaaggaag aagaacaaga gcaggctgaa
    17161 caagaagctg agagtaagaa agaaccttac atctactaca tcctttcttt tgctaagttg
    17221 gctgacttgg tagcttttgc caagacagtg acttttgaaa tggaaacttc tgaactctac
    17281 aaaatgaacg agcgctatta tttgaccatt ttagtggata ttgaaaatca tccaagccca
    17341 tatccagctt ggctgttggc ccgtatgcgc gagtttgcag aggatagtga tatcagtcgc
    17401 tcagtcttac aagagtatgg tcaagttttg atgagtcacg atgcagtgct caatctgcaa
    17461 aaaatcggct aatcttttct ggaaagctat tttcagattt taagaagagc gaatcgtctg
    17521 attcgttttt tctttttata ctgaaatagt gatttactat aataggaatt ttcacaaaat
    17581 tctgttataa tggctatatt agaaaatttc gaggagataa agatgacagt taaaattgct
    17641 ttactaggat ttggtaccgt tgcaagtggt gtgcctttcc tcctaaagga aaatggagga
    17701 aaaatcaatc aatcagcaca ttcagatatc aaagttgcta aggtattggt caaggatgaa
    17761 gatgaaaaaa atcgcttgct tgcagcaggg aatgacttta actttgtaac caatgtggat
    17821 gatattttat cagaccagga tattactatc gtagtggaat tgatggggcg tattgagcct
    17881 gctaaaacct ttatcactcg tgccttggaa gctggaaaac acgttgttac tgctaacaag
    17941 gaccttttag ctgtccatgg cgcagaattg ctagaaatcg ctcaagctaa caaggtagca
    18001 ctttactacg aagcagcagt tgctggtggg attccaattc ttcgtacttt agcaaattcc
    18061 ttggcttctg ataaaattac gcgcgtgctt ggagtagtca acggaacttc caacttcatg
    18121 gtgaccaaga tggtggaaga aggctggtct tacgatgatg ctcttgcgga agcacaacgt
    18181 ctaggatttg cagaaagcga tccgacgaat gacgtagatg ggattgatgc agcctacaag
    18241 atggttattt tgagccaatt tgcctttggc atgaagattg cctttgatga tgtagtccac
    18301 aagggaatcc gcaatatcac accagaagac gtagctgtag ctcaagagct tggttacgta
    18361 gtgaaattgg ttggttctat tgaggaaact tcttcaggta ttgctgcaga agtgactcca
    18421 accttcctac ctaaagcgca cccacttgct agtgtgaatg gcgtaatgaa cgctgtcttt
    18481 gtagaatcta tcggtattgg tgagtctatg tactacggac caggtgcggg tcaaaaacca
    18541 actgcaacaa gtgttgtagc tgatattgtc cgtatcgttc gtcgtttgaa tgatggtact
    18601 attggcaaag acttcaacga atatagccgt gacttggtct tggcaaatcc tgaagatgtc
    18661 aaagcaaact actatttctc aatcttggct ccagactcaa aaggtcaggt cttgaagttg
    18721 gctgaaatct tcaatgctca agatatttcc tttaagcaaa tccttcaaga tggcaaagag
    18781 ggtgacaagg cgcgtgtcgt tatcatcaca cacaagatta ataaagccca gcttgaaaat
    18841 gtctcagctg aattgaagaa ggtttcagaa ttcgacctct tgaatacctt caaggtgcta
    18901 ggagaataag atgaagatta ttgtacctgc aaccagtgcc aatatcgggc caggttttga
    18961 ctcggtcggt gtagctgtaa ccaagtatct tcaaattgag gtctgcgaag aacgagatga
    19021 gtggctgatt gaacaccaga ttggcaaatg gattccacat gatgagcgta atctcttgct
    19081 caaaatcgct ttacaaattg taccagactt gcaaccaaga cgcttgaaaa tgaccagtga
    19141 tgtccctttg gcgcgcggtt tgggttcttc cagctcggtt atcgttgctg ggattgaact
    19201 agccaaccaa ctgggtcaac tcaacttatc agaccatgaa aaattgcagt tagcgaccaa
    19261 gattgaaggg catcctgaca atgtggctcc agccatttat ggtaatctcg ttattgcaag
    19321 ttctgttgaa gggcaagtct ctgctatcgt agcagacttt ccagagtgtg attttctagc
    19381 ttacattcca aactatgaat tacgtactcg cgacagccgt agtgtcttgc ctaaaaaatt
    19441 gtcttataag gaagctgttg ctgcaagttc tatcgccaat gtagcggttg ctgccttgtt
    19501 ggcaggagac atggtgaccg ctgggcaagc aatcgaggga gacctcttcc atgagcgcta
    19561 tcgtcaggac ttggtaagag aatttgcgat gattaagcaa gtgaccaaag aaaatggggc
    19621 ctatgcaacc tacctttctg gtgctgggcc gacagttatg gttctggctt ctcatgacaa
    19681 gatgccaaca attaaggcag aattggaaaa gcaacctttc aaaggaaaac tgcatgactt
    19741 gagagttgat acccaaggtg tccgtgtaga agcaaaataa agaatagaag ataggatggg
    19801 gaaactcttg accagagggt ttcatatcct ttttgtgaaa agaagtttat actcaatgaa
    19861 aatcaaagag caaactagga agctagccgc aggctgctca aaacagtgtt ttgaggttgc
    19921 agatagaact gacgaagtca gtaacatata tacggtaagg tgacgctgac gtggtttgaa
    19981 gagattttcg aagagtatta gttaaaaacg tgataaagga gaaataaaga tggcagaaat
    20041 ttatctagca ggtggttgtt tttggggcct agaggaatat ttttcacgca tttctggagt
    20101 gctagaaacc agtgttggct acgctaatgg tcaagtcgaa acgaccaatt accagttgat
    20161 caaggaaaca gaccatgcag aaacggtcca agtgatttac gatgagaagg aagtgtcact
    20221 cagagagatt ttactttatt atttccgagt tatcgatcct ctatctatca atcaacaagg
    20281 gaatgaccgt ggtcgccaat atcgaactgg gatttattat caggatgaag cagatttgcc
    20341 agctatctac acagtggtac aggagcagga acgcatgctg ggtcgaaaga ttgcagtaga
    20401 agtggagcaa ttacgccact acattctggc tgaagactac caccaagatt atctcaggaa
    20461 gaatccttca ggttactgtc atatcgatgt gaccgatgct gataagccat tgattgatgc
    20521 agcaaactat gaaaagccta gtcaagaggt gttgaaggcc agtctatctg aagagtctta
    20581 tcgtgtcaca caagaagctg ctacagaggc tccatttacc aatgcctatg accaaacctt
    20641 tgaagagggg atttatgtag atattacgac aggtgagcca ctcttttttg ccaaggataa
    20701 gtttgcttca ggttgtggtt ggccaagttt tagccgtccg atttccaaag agttgattca
    20761 ttattacaag gatctgagcc atggaatgga gcgaattgaa gttcgttctc gttcaggcag
    20821 tgctcacttg ggtcatgttt tcacagatgg accgcgggag ttaggcggcc tccgttactg
    20881 tatcaattct gcttctttac gctttgtggc caaggatgag atggaaaaag caggatatgg
    20941 ctatctattg ccttacttaa acaaataaaa cagagagtgg ggcttcccac tttcttcatt
    21001 tctagaatat gaatagaagg gatttatgaa acacctatta tcttacttca aaccctacat
    21061 caaggaatca attttagccc ccttgttcaa gctgttagaa gctgtttttg agctcttggt
    21121 tcccatggtg attgctggga ttgttgacca atctttacct cagggagatc aaggtcatct
    21181 ctggatgcag attggcctgc tccttatctt tgcagtaatt ggcgttttag tggccttgat
    21241 agctcaattt tactcagcaa aggcagcagt aggttttgct aaggaattga caaacgatct
    21301 ttatcgtcat attctttcct tgcccaagga cagcagagac cgtctgacaa catctagttt
    21361 ggtcactcgc ttgacttcgg atacctacca gattcagact ggtatcaatc aattcctgcg
    21421 tctctttttg cgagcgccca ttatcgtttt tggtgccatt tttatggctt atcgaatctc
    21481 aactgagttg actttctggt tcttagtcat ggttgccatt ttgaccattg tcattgtagg
    21541 gttatctcga ttggtcaatc ctctctacag tagtctcaga aagaaaacgg accaactggt
    21601 tcaggaaacg cgccagcaat tgcaagggat gcgggttatt cgtgcttttg gtcaagaaaa
    21661 acgagagtta cagatttttc aaacccttaa ccaagtttat gctagattac aagaaaagac
    21721 aggtttctgg tctagtttat taacacctct gacctatctg attgtcaatg gaactcttct
    21781 cgttattatc tggcaaggct atatttcaat tcaaggagga gtgctcagtc aaggtgctct
    21841 cattgctctt atcaattacc tcttacagat tttggtggaa ttggtcaagc tagccatgtt
    21901 gatcaattcc ctcaaccagt cctatatctc agtcaagcga atcgaggaag tctttgttga
    21961 ggctccagag gatatccatt cagagttaga acaaaagcaa gctaccagag ataaggtttt
    22021 acaagtccaa gaattgactt ttacctatcc tgatgcggcc cagccttctc tgagatacat
    22081 ttcctttgat atgactcaag gacaaattct aggtatcatc gggggaactg gttctggtaa
    22141 atcaagcttg gtgcaactct tacttggact ttatccagta gacaagggga acattgacct
    22201 ttatcaaaat ggacgtagtc ctcttaattt ggagcagtgg cggtcttgga ttgcctatgt
    22261 acctcaaaag gtcgaactct ttaaaggaac cattcgttcc aacttgactc taggtttcaa
    22321 tcaagaagta tctgaccagg aactctggca ggccttggag attgcgcaag ctaaggattt
    22381 tgtcagtgaa aaggaaggac tcttggatgc tctagttgag gcaggggggc gaaatttctc
    22441 aggtggacaa aaacaaagat tgtctatcgc ccgagcagtc ttgcgccaag ctccgtttct
    22501 catcctagat gatgcaacct cggcactgga taccattaca gagtccaagc tcttgaaagc
    22561 tattagagaa aattttccaa acacgagctt aattttgatc tctcaacgaa cctcaacttt
    22621 acagatggcg gaccagattc tcctcttgga aaaaggtgag ttgctagctg ttggcaagca
    22681 cgatgacttg atgaaatcca gccaagtcta ttgtgaaatc aatgcatccc aacatggaaa
    22741 ggaggactag aatgaaacga caaactgtaa accagacgct caaacgttta gccgtagatt
    22801 tagcaagcca tcctttcctc cttttcctag cctttctagg aactattgct caagttggct
    22861 tatcaattta cctacctatt ctgattgggc aggtcattga ccaagtccta gtggctggtt
    22921 catcaccagt tttttggcag atttttctcc agatgctctt ggtggtaata ggaaatactc
    22981 tggtacaatg ggccaatcct ctcctctata atcgtctaat cttctcttat accagagatt
    23041 tacgggagcg aatcatccat aagctccatc gtttaccgat tgcctttgta gataggcaag
    23101 gtagtggaga gatggttagt cgtgtaacca cggacatcga acagttggca gctggcttga
    23161 ccatgatttt taaccaattt ttcattggtg ttttgatgat tttggtcagt attctagcca
    23221 tgctccaaat tcatctcctc atgactctct tagtcttgct gttgacgcca ctgtccatgg
    23281 tgatttcacg ctttattgcc aagaaatcct atcatctctt ccagaagcaa acagagacga
    23341 ggggaattca gactcagttg attgaagaat cgcttagtca gcagactata atccagtcct
    23401 tcaatgctca aacagaattt atccaaagat tgcgtgaggc tcatgacaac tactcaggct
    23461 attctcagtc agccatcttt tattcttcaa cggtcaatcc ttcgactcgc tttgtaaatg
    23521 cactcattta tgccctttta gctggagtag gagcttatcg tatcatgatg ggttcagcct
    23581 tgaccgtcgg tcgtttagtg acttttttga actatgttca gcaatacacc aagcccttta
    23641 acgatatttc ttcagtgcta gctgagttgc aaagtgctct ggcttgcgta gagcgtatct
    23701 atggagtctt agatagccct gaagtggctg aaacaggtaa ggaagtcttg acgaccagtg
    23761 accaagttaa gggagctatt tcctttaaac atgtctcttt tggctaccat cctgaaaaaa
    23821 ttttgattaa ggacttgtct atcgatattc cagctggtag taaggtagcc atcgttggtc
    23881 cgacaggtgc tggaaaatca actcttatca atctccttat gcgtttttat cccattagct
    23941 cgggagatat cttgctggat gggcaatcca tttatgatta tacacgagta tcattgagac
    24001 agcagtttgg tatggtgctt caagaaacct ggctcacaca agggaccatt catgataata
    24061 ttgcctttgg caatcctgaa gccagtcgag agcaagtaat tgctgctgcc aaagcagcta
    24121 atgcagactt tttcatccaa cagttgccac agggatacga taccaagttg gaaaatgctg
    24181 gagaatctct ctctgtcggc caagctcagc tcttgaccat agcccgagtc tttctggcta
    24241 ttccaaagat tcttatctta gacgaggcaa cttcttccat tgatacacgg acagaagtgc
    24301 tggtacagga tgcctttgca aaactcatga agggccgcac aagtttcatc attgctcacc
    24361 gtttgtcaac cattcaggat gcggatttaa ttcttgtctt agtagatggt gatattgttg
    24421 aatatggtaa ccatcaagaa ctcatggata gaaagggtaa gtattaccaa atgcaaaaag
    24481 ctgcggcttt tagttctgaa taagccattc tcttttgaaa gtttatggac gaaaaaagtt
    24541 gccttcgagt gacttttttg ttacaatagc tagaaaaatt gttcactgta atactcaatg
    24601 aaaatcaaag agcaaactag gaagctagcc gtaggttgct caaagcacag ctttgaggtt
    24661 gtagataaga ctgacgaagt cagttcaaaa cactgttttg aggttgcaga tagaactgac
    24721 gaagtcagct caaaacactg ttttgaggtt gcagatagaa ctgacgaagt cagtaaccac
    24781 acctacggca aagtgaatct gaagtggttt gaagagagta caacttgtct tttagaaaag
    24841 gagcctataa tgaaagtctt tcagcatgta aatatcgtga cttgtgatca agatttccat
    24901 gtttatcttg atggaatctt agcagtcaag gattctcaaa tcgtctatgt cggtcaagat
    24961 aagccagcgt ttttagagca agctgagcag attatagact atcagggagc ttggattatg
    25021 cctggtttgg tcaattgtca cacccattct gcaatgacag gtctgagagg gatccgagat
    25081 gacagcaatc tccatgaatg gctcaatgac tatatttggc cagcagaatc tgagtttact
    25141 cccgacatga ctaccaatgc ggtcaaagaa gccctgacag agatgctcca gtcaggaaca
    25201 acaaccttta acgatatgta taatcccaat ggtgtggata tccagcaaat ttatcaggtg
    25261 gtgaaaactt ccaagatgcg ttgttatttt tctccgactc tcttttcttc agagacagaa
    25321 acaactgctg agactataag tagaactcga tccatcatag acgaaatctt aaaatataaa
    25381 aatccaaatt tcaaggttat ggtagcacct cattctccgt atagctgcag tagagacttg
    25441 ctggaagcga gtttggaaat ggcaaaagag ctaaatattc cgctccatgt ccatgtggcg
    25501 gagaccaagg aggagtcagg aattatcctc aaacgatacg gcaaacgccc ccttgctttt
    25561 cttgaagaac tgggttattt agatcatccg tccgtatttg ctcacggggt cgaattaaac
    25621 gagagagaaa ttgaacgctt ggcatcttct caagtggcta tcgcccacaa tcctatcagt
    25681 aacctcaaac tggcatcagg aattgctcca attatccagc tccaaaaagc gggagtagca
    25741 gtcggaattg cgactgactc ggttgcttcc aataacaatc tagatatgtt tgaggaagga
    25801 aggactgcag ctcttcttca gaagatgaaa agtggggatg ccagccagtt tccaatcgaa
    25861 acagctctca aggtactgac aatcgaaggg gctaaggtcc ttggaatgga aaatcagata
    25921 ggaagtctgg aagtcggcaa gcaagcagat tttctggtca ttcaaccaca agggaaaatt
    25981 catctccaac ctcaggaaaa tatgctgtct cacctggttt atgcagttaa atctagtgat
    26041 gtagatgatg tttatatcgc cggagaacag gttgttaagc aaggtcaagt cctgacagta
    26101 gaactttaaa agaaaaatta cgaaaaattt taaaaaaagt ttgcaaaaat cttgcattct
    26161 ttttttgact atgctatact tatatacggt ttaaaaaaac tgcctaagac agtaggggag
    26221 ctcgactcat aaatatccta ccgaggacaa aacgtatcat gtaaaaagaa gcgtattgta
    26281 ctttcgtgtc taggtttggg cgcgtttttc tttttgaaaa attccccaag caaaataatt
    26341 acggaggtga acacactaat gagtgaagca attattgcta aaaaagcgga actagttgac
    26401 gtagtagctg aaaaaatgaa agctgctgca tctatcgtcg ttgtagacgc tcgtggtttg
    26461 acagttgagc aagatacagt tcttcgtcgt gagcttcgtg gaagcgaagt tgagtataaa
    26521 gttattaaaa actcaatctt gcgtcgtgca gctgaaaaag ctggtcttga agatcttgca
    26581 tctgtatttg ttggaccatc tgcagtagca ttttctaatg aagatgttat cgcaccagcg
    26641 aaaatcttga acgacttttc taaaaacgct gaagcacttg aaattaaagg tggtgcaatc
    26701 gaaggcgctg tcgcatctaa agaagagatt cttgcacttg caactcttcc aaaccgcgaa
    26761 ggacttcttt ctatgctcct ttctgtactt caagcgccag tgcgcaacgt tgctcttgca
    26821 gtcaaagcgg ttgcagaaag caaagaagac gcggcttaat cttaagctac acagcgtagc
    26881 ctagctacga aaaaaactat tataaaattt aaaacttatt tggaggaaat aacaatggca
    26941 ttgaacattg aaaacattat tgctgaaatt aaagaagctt caatccttga attgaacgac
    27001 cttgtaaaag ctatcgaaga agaatttggt gtaactgcag ctgctcctgt agctgttgct
    27061 gcagctgatg cagctgatgc tggtgctgct aaagattcat tcgacgttga attgacatct
    27121 gcaggcgaca aaaaagttgg cgttatcaaa gttgtacgtg aaatcactgg tcttggtctt
    27181 aaagaagcta aagaacttgt tgacggtgca ccagcacttg ttaaagaagg cgttgcaact
    27241 gcagaagctg aagaaatcaa agctaaattg gaagaagctg gagcttcagt tactcttaaa
    27301 taatagagta tcgcaatttg attgcggaat ccctatttac cagtctttga gagcgataag
    27361 cgattgatag aaactgataa attgatttgg tttcctgcca aaaattcttt ggcaggattt
    27421 ttgttttaat aaactttcgg gggtagacac tagattgtct accccttttt ttgtgtttcg
    27481 gaggtaatta tgcaaaagga aggacagtca tcacgtcaat tcgtgaagag tcatctcatg
    27541 actatcatgg gagttgatat tgtaaaagca actcagttag ttgatgaaat ggaacaagta
    27601 ggtttaatcc gatttgatga atttggaaat gttggaattt tagtcttgga gggacaataa
    27661 tgaagcgtat taccgctaat caatatcaga catcagagcg ttattataag ctccccaaac
    27721 ttttatttga gagtgaacgg tataagaata tgaaactgga ggttaaggtg gtttactcag
    27781 ttttaaaaga tcggttggag ttgtctttga gcaaaggttg gattgatgag gatggggcta
    27841 tttatttgat ttattccaat tcaaatttga tggcactttt aggctgttca aagtcaaaat
    27901 tactctccat gtgagtttga agtgatattt ttagatgatt accataaaaa acataactac
    27961 ccactatttt acgaatccta tcttcaaaac gttatggaat tccttgaaag tcaagacata
    28021 aagaatgggg ttgatgcctt tgtagatgat catcaaaatc tcgtttttgt tttatatgga
    28081 caaggctatc gagccgaggg aaaagaggga atacttacaa cccaagtaac tgtaaaagct
    28141 tatgatgaag acaagaaacc gattaacttc gcaaatttat tagattcctt aatttattag
    28201 attccttaat cgtgtcagaa tatcaaatgg aaccgaatct ttgggaggtc tcccatgatt
    28261 gatctctatc taagtaaaaa tagacaaaga aatcaacttc ttttagattt cttccaaaac
    28321 tatggcatag aggtatcttg tcattcagtt tctgaaatga caaaggacaa attaattgag
    28381 atgatgagct attcttcaga ttgttttgag tttttatctc ccaatttatt acgatttaag
    28441 aaccgtgaca atctaagatt aacggatttc gttgagataa ttttaaaaaa tcctgaacta
    28501 accatcagac ttcctcttgc ggtttcaaat aagcgagttt atccaagtct gaatttggaa
    28561 gaggctagag ctctattgcc aagagatacg aaacaattga tttacatggc gcaaacacat
    28621 tatttatcta actaaaggag aagcaatatg gctgaacgat tttgggagaa cttgtccatt
    28681 attttggctg aaagaaatat cagctggatt gagttaacca gaaaaatgtt tgcgggagag
    28741 tttcactatc caagtgaatt gaatcgtttg tatcaaaaga ttcgccacta gaagatggaa
    28801 caatgaatgc ctcaaagtcc atgggtagaa aggattgtgc aggtattaga tttagattat
    28861 gaagatttat ttcggaggta actatgaaaa gaataattcc agtttatata ttccaacaag
    28921 taaatgtcct attggtatct ctatacttat tgaaatttct ttgtatcggt gagttaacta
    28981 tactacagat tctctatggt tcgtcactca tttctttttt atggatgtat ggccaacgca
    29041 aacaagcgca taaggtcaat atgaaatcta ggatgaaatg gcttggtgtt gaatttgtta
    29101 gcctactgat tataagtcta tgttttagtc tgattcatgc tcaaggaagc acgaatcaag
    29161 caaacttaat tggccttcaa catcaaattc cttggttttc atttttattg ttcttaatca
    29221 atgcgagtat ggttgaagaa tttctgtatc gagaaatttt atggaatttg gtcagaaaat
    29281 tagatatcag agttgctttg acaagtgttt tatttgcctt agcacatcat ccaggaacca
    29341 ttatagcttg gtgtttgtat gtatcgctgg ggatgttttt agggcttgta cgctacaaat
    29401 tggacttatg gggcagtatg gggctacatt tggtgtggag cctactagtt tatagtttgc
    29461 tgctttttta aaataggtaa cctgattttt aggtaacttc taaaactaac ttccagtttt
    29521 gggagagaga tggaagttac tttgaaaagt tacggaaaca ttattttcta cgtgacaaca
    29581 ttgtatcatt gcttgcattt tactgctgga gagagtataa tgaaggtgga taaagcactt
    29641 aatcccaaaa atttaaatag gaggagttag ctatggcaga agaagtatta aatcttcaat
    29701 tagtatctgt tcaagttgac gaaacagatg aagttgatgg aatgcgattt agtacattca
    29761 gtacaaaccg ttgcggtaac tggagtgctt ttagttggga aaattgttaa ttcatttaaa
    29821 ttgtaaactt tagggattct ctgaagttat ggatgtcgtt ccaatatgtg cacgttggaa
    29881 tgttagtgct tatatggggg gaacagaatc ctctcttgat tgaagacaag ttagccatta
    29941 ggctggtttg tctctttgtc aactgtagtg ggttgatata atagtattag tgagtgggat
    30001 aaaagtttca tttagtttat tcagtacaaa tttaacgggt caagatttat tgaactgcac
    30061 cccaatagtt agacagaa
//
LOCUS       DAVLXF010000016        28910 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLXF010000016 DAVLXF010000000
VERSION     DAVLXF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28910
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.016"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..2911)
                     /locus_tag="VYO12_000660"
     CDS             complement(<1..2911)
                     /locus_tag="VYO12_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1587308.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTD"
     gene            complement(2921..5215)
                     /locus_tag="VYO12_000661"
     CDS             complement(2921..5215)
                     /locus_tag="VYO12_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1587309.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(5274..6074)
                     /locus_tag="VYO12_000662"
     CDS             complement(5274..6074)
                     /locus_tag="VYO12_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1587310.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(6071..6844)
                     /locus_tag="VYO12_000663"
     CDS             complement(6071..6844)
                     /locus_tag="VYO12_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1587311.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(6869..7339)
                     /locus_tag="VYO12_000664"
     CDS             complement(6869..7339)
                     /locus_tag="VYO12_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1587312.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(7330..7761)
                     /locus_tag="VYO12_000665"
     CDS             complement(7330..7761)
                     /locus_tag="VYO12_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1587313.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEGDLCL"
     gene            complement(7754..8194)
                     /locus_tag="VYO12_000666"
     CDS             complement(7754..8194)
                     /locus_tag="VYO12_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1587314.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(8206..8844)
                     /locus_tag="VYO12_000667"
     CDS             complement(8206..8844)
                     /locus_tag="VYO12_000667"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1587315.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            complement(8959..10362)
                     /locus_tag="VYO12_000668"
     CDS             complement(8959..10362)
                     /locus_tag="VYO12_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1587316.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            10538..11311
                     /locus_tag="VYO12_000669"
     CDS             10538..11311
                     /locus_tag="VYO12_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1587317.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(12439..13944)
                     /gene="adcA"
                     /locus_tag="VYO12_000670"
     CDS             complement(12439..13944)
                     /gene="adcA"
                     /locus_tag="VYO12_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1587318.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVTKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYVEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(13954..14760)
                     /locus_tag="VYO12_000671"
     CDS             complement(13954..14760)
                     /locus_tag="VYO12_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1587319.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(14753..15457)
                     /locus_tag="VYO12_000672"
     CDS             complement(14753..15457)
                     /locus_tag="VYO12_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1587320.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVSHA"
     gene            complement(15457..15897)
                     /gene="adcR"
                     /locus_tag="VYO12_000673"
     CDS             complement(15457..15897)
                     /gene="adcR"
                     /locus_tag="VYO12_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1587321.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(16052..17320)
                     /gene="dltD"
                     /locus_tag="VYO12_000674"
     CDS             complement(16052..17320)
                     /gene="dltD"
                     /locus_tag="VYO12_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1587322.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(17313..17552)
                     /gene="dltC"
                     /locus_tag="VYO12_000675"
     CDS             complement(17313..17552)
                     /gene="dltC"
                     /locus_tag="VYO12_000675"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1587323.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(17566..18810)
                     /gene="dltB"
                     /locus_tag="VYO12_000676"
     CDS             complement(17566..18810)
                     /gene="dltB"
                     /locus_tag="VYO12_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1587324.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(18807..20357)
                     /gene="dltA"
                     /locus_tag="VYO12_000677"
     CDS             complement(18807..20357)
                     /gene="dltA"
                     /locus_tag="VYO12_000677"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1587325.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKSDSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     ITDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDSVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(20378..20509)
                     /locus_tag="VYO12_000678"
     CDS             complement(20378..20509)
                     /locus_tag="VYO12_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1587326.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(20740..21444)
                     /locus_tag="VYO12_000679"
     CDS             complement(20740..21444)
                     /locus_tag="VYO12_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1587327.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPISNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(21514..23340)
                     /gene="glpO"
                     /locus_tag="VYO12_000680"
     CDS             complement(21514..23340)
                     /gene="glpO"
                     /locus_tag="VYO12_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1587328.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTSAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            complement(23382..24890)
                     /gene="glpK"
                     /locus_tag="VYO12_000681"
     CDS             complement(23382..24890)
                     /gene="glpK"
                     /locus_tag="VYO12_000681"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1587329.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(25049..26476)
                     /locus_tag="VYO12_000682"
     CDS             complement(25049..26476)
                     /locus_tag="VYO12_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1587330.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSENENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            26641..27513
                     /gene="hslO"
                     /locus_tag="VYO12_000683"
     CDS             26641..27513
                     /gene="hslO"
                     /locus_tag="VYO12_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1587331.1"
                     /translation="MNKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            27500..28480
                     /gene="dusB"
                     /locus_tag="VYO12_000684"
     CDS             27500..28480
                     /gene="dusB"
                     /locus_tag="VYO12_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1587332.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(28710..>28910)
                     /locus_tag="VYO12_000685"
     CDS             complement(28710..>28910)
                     /locus_tag="VYO12_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV1587333.1"
                     /translation="NANGSMATGWLKDGDTWYYLEASGAMKESQWFKVSDKWYYVNGS
                     GALAVNTTVGGYRVNANGKWVN"
BASE COUNT         8732 a         6376 c         4985 g         8817 t
ORIGIN      
        1 catctgttct attataaata attaactttt ctaattcttc ggtttgtgct aaatctatct
       61 cccaccatga aggagaatct tctttggtat gagtcactga atgatgacca taatctccgt
      121 ttttatttcc atcaactgct agacgagaaa aaccattgta atccgttgaa ctctgtcgag
      181 tttcttttgt taatgcaata ttagaaagtt tcttcggagt tctctttgaa ccatagactt
      241 caacttccgc taaacttaac ggaatagctg ctgattttaa ttctattttg actagcttag
      301 cccctacaga aggtactttt aaagataaca aaccatcaac tactttatca aaatgtcttc
      361 taaaaacttc ttctcctgat gaagatagaa aaataacatc aaaattagat aaacgctgag
      421 gttcggcatc tgttctatta taaatatcaa ctttagaaac cgtaaactct tctcccagat
      481 cgacctgcca ccaagcgtta gaatctgcct tagtatgagt caccgaatga tgtccgtaat
      541 ctccatcttt attaccatca actgctaaag cagctacacc ctcataattg gttgaacttt
      601 gagtagctac cttatttgta gagactactt tatcttctgt tattttatct atatcctctt
      661 cagattgctt accatctgat tctctaaaaa cctctacttc tgctaaactc aaaggcactc
      721 cactcgttag taatttaact ttaatatacc ttgctccttt ttctttgaaa tctagactaa
      781 cagattcacc cgacaaatta ttaacatgtt tcttagcaac ttcgtttcta ttattgtcat
      841 atagaatcac atcaaaatta gatagacgtt gagtctcagc atctgtgcga ttataaattt
      901 taacaagccc aactttatcc atttttttca aatcgacttc ccaccaagag ggattatctg
      961 ccctagtgtg tgttaccgaa ccagagttaa aattaccatt tctgttacca tccacagctc
     1021 tattagggtc tccattgtaa tctacagaac tttgtgttgt tggttttgca tatgctatat
     1081 tcaaattacc atcattaaat ttatctggtg ttgggaaaga gactggacct gtcccctcag
     1141 tatttataga catctctacc attccattat gattaaccgt aatggtataa acatgggtat
     1201 tctcatccca attttctgta taagtataat gatttttatc gccacttata tttatctgag
     1261 gtttatgacc agtatgccct tttaatgtaa gtgttgttgt cctaaagtca ttatctacag
     1321 gattgatgga ataatttttg cttatccagt tcgctaactc taattcttct ttcttccaac
     1381 tttttgatgc atcaaaattt cctgataatg cccacatagc tgtcttatct gtcctgtaat
     1441 tattcaataa aatatgtaaa ttatttggat tttctttaac aacagcgtaa gtatgtggcg
     1501 tcaaatctaa cgataacgac tttgtattat tagtatacat aggcaacatt acttgctgat
     1561 ttttattgat attagcatta ctattataaa tataccagga attacctaca cgctgagcat
     1621 acccatctcc ttcataaagt tttggataaa gcgagtttaa atagttgact ttactagaca
     1681 attcctcact atttttagtc aaaatttttg cattagggaa tatagatgaa atcttttcct
     1741 tatcaatttt ctcatgtatt acaggaagaa tatgatatct cccattatta tataaaggca
     1801 ttgtttcatc attcgaataa agtccttgat aaaatccgtt taatgaacta atcctacctt
     1861 ctccattcca aaatacagct tttgttctat ttacaacttc ttccttactt ggagctggat
     1921 tttgtatagc atgtctaaag aaaggaataa tacctttagt aaatgctgga gttggtacat
     1981 catttgtcat aaatgtatac gcggcacatt cgaaattata aactgtgcct cccccagtat
     2041 atacattcat catttccata gcaatcattg attctggttc cgatgcatag gatctcatat
     2101 ccctagctct tccagtttca aatgtgtttg tataatgttt ttcccaccat ttccatgtat
     2161 ctgttgatga gccccagtta tcacataagc cactcaacca aaatccacta acgatagaat
     2221 ctgtacccgc atcatctctt attggcgtat ttttagttgc caacaccaaa tttttatgat
     2281 atttttgact cgcttcaaag aatgtcggat cattcataat agtttcccag aaccattttt
     2341 catgatcatg ccagataaaa tgcgctccat atttggcaca aacttccaaa tatttagcac
     2401 tatgcggagc taactggtta ttgtaaatcc aataattctc aatatttaaa acacctttta
     2461 acacactata cttttggaat tgttcatcta accactctgg aggaactgta ttacgctctc
     2521 cagccgacat aataaccaag aaaactggaa tgtttagact ttgagcttcc tccaacatct
     2581 tgacatacca ttctctcaat tctttcgtat ctcgtggaat acaacttgtt ggtttacaga
     2641 cttttgcagg atgaagttca atcgctgcat atggctttac atcttcagga atagcttccc
     2701 atgctccctt caatgtgttc ccccaccata atccgttacc attatcattg ccatacaacg
     2761 gagaaagcaa caatggcgat tcattattaa tcgtcgttct catttgaaca cgattatcag
     2821 ccttaactcc tgctaacaat ccaaaacata acatcccaat acatacaaac aatattgtga
     2881 ttaattttct ttttattttt tctttattca tgacttatac ctaaaaattt aactcaataa
     2941 ttttttctga attaaatacc aattcaatat tttgtacatc agtatttttg ccatatattc
     3001 ttactcttac tttttgatct ttgtttcctc cttccaattt taggaatgtt atatccccat
     3061 ttttccaagc aaacgatacc ttatatcctc ctcttactct gaaacctttc acttctcctt
     3121 ctgaccaagc agaaggtaaa gctggaatta gtgataacca attatgatgg ctctgtacta
     3181 ataattcaca aattccactc accaaaccta aattaccatc aatttgaaat ggtggatggt
     3241 caagaaataa attgccaaga gtcgcattat ttaacaaacc attaatctgg ttataagcag
     3301 gttcaccttg atatagtctc gcaaaaaaat gaatcagcca tgcagcactc caacctgttt
     3361 gtgtactagc atgcaaacca cttactaacc aattattaat cgcttgctcc ctctcctgtg
     3421 aagataaaaa attagcgttt gataatctcc tattgatagt gattttagct gcttctgcta
     3481 attccggagt tttatgaata tcaatctcat tataaggata aagcccaaat agaggtgaaa
     3541 tgtgtctatg cccaggctct acttcttcat aatcttctaa ccattcttgg atttgcccat
     3601 tactacctat ttttgtttta ggtagtttct tttttaactc cttcacacga ctaataaaat
     3661 ccgaattgtc tcctaattgt tttgcaatgc caatgcatga atcacaaaaa tatcttagaa
     3721 tttgattatc aattgtagat gatagacaag catttccttc aataccattt tttaagcgat
     3781 atttattttc cggtgagaca cttggacctg tcatcaagta gccatccacc tcaaataaat
     3841 aatcttcaaa gaaaagaaat gcttctttta tcatttcaaa atgttccgta agaatacgct
     3901 catcttggaa atataaatag tgttcccaaa tatgagtaca taaccatgga atagttaata
     3961 cccaaattgc agcccccatg gcatgagatt ggggagccgt atcgccaaaa ccatccgtat
     4021 tatgatgtgc tgtaaaacct ctagctccat acattttctt agcggttagt cttcccggtt
     4081 ctctcattct ttcgagcata tcaaataatg gatattctac ttctggtaaa tcacatggac
     4141 ctaccatcca ataattcatt tgagtattaa tattaatcgt atatttagaa ccccaaattg
     4201 gatttaattc atcacaccat attccttgaa gattggcagg taaaccattc ggttgactag
     4261 acgatattaa cagatatctt ccataatgaa ataacaagtt agtcaagtag ttactatact
     4321 ttttagtgtt ttcaagaagt agattcgttg gaatgctaag acaatcttta ctatagtcta
     4381 gtttaaaatc aactctatta aattgctcct gatatttttt tacatgttca tctttttctg
     4441 taaagtaatc aatactacta aattctccct gaagagaaga aatatctata tttccccaat
     4501 aatccgtcat tgatttgaga taaagaaata cctctgtagc attccgaata actattgtct
     4561 ctcccaatac acttacttca ccatccgtaa ccttagaatg acatactact ttaaactgaa
     4621 caccttttct acctccagca gaggccgaca ttaaaattgt acttgaatcc agtttagata
     4681 cttcgtcatt aaaccgttta tttctaccca aattaatgtt taaatttaat gtgttttgaa
     4741 ctgatgacac tatacggcaa cataaaatat tcttattaaa actcgtaaaa tattctcttt
     4801 ttatttgtaa attacaacta ttaggctcaa acacaacatt agaaatagct gtatctaaat
     4861 ctagctctct ttcatacaat gaaagagcac aagactgaat atctatatgc tcaatgtaaa
     4921 gttccccaag taattcatag tggctttgat ctcttggggt agcaaacatt gttaacttta
     4981 tcaattcttc ggctttctga atttctccat ctaaaagata ttcccgaatt tttttaagat
     5041 gcaatagtga gtctggatta tttctatctg actttcctct ataccaaata gtctcatcgt
     5101 ttagttgaat acattctttt gtagctgaac cataaatcat accacctaaa tgaccgttcc
     5161 caatcggcaa ggcttcattc caatttgagg cagctttctt ataccataat ttcactcttt
     5221 acctctcttc caaaatggag gtgatatccc aaataccacc tccacacttt ctactacttc
     5281 aaaattccta aaacaactag gataactcca atagccataa cactaaagat tagtacaacc
     5341 ggattctttc cttttctaat taatttacac attaataaag tcaaagccat agggataaat
     5401 cctggaatta attttgtaat catttcttga acagaaataa caagttcccc ctgcttaaat
     5461 tctaatccaa aattaatacc aaccattgat ggaatcaaac cacccactag tactagccct
     5521 aatgctgtcg ccatactcgt aacgcgattc aatgttcctt ttgtattatt ttcttggata
     5581 agactagaac ccttagtata cccatatttc aaaccgaaat atttaacagg aatattaata
     5641 atattaaaca atattaaggc gataaagata cctaaagcac cgccgtcttt agaataagac
     5701 gcaccaatac taaaacagat aggaactagt gtcagccaga acaaactatc tcctagacca
     5761 gccagtggcc ccatcaagcc aactttaata ccagtaattg aagctgcacc ttcatttcct
     5821 tcttgttctt ccatagcgga agtaactcca agaataaatg gtgccgctgt ttgatgagta
     5881 ttgaaaaact caaggtgacg tttcattgca ggagaagctg aatctttgtc tgggtatagt
     5941 tttttcaata ctggaagaat tgtatataga aaacctaggt tctgcattct ctcatagttc
     6001 catgaagaac catacataaa acttcgtttg aaaacttttt taagttcaac tttagttact
     6061 ttattattca tcatagatca agatcctcct caacatcact acttgaagtt gtttcttgtg
     6121 gtttacttcc aatcatatcg tagaagtatg ctaccgcaat accaactagt gagatcccaa
     6181 tggttgacat tcctccatac acagaacaaa tgaatccaat caacaagaat acccacatat
     6241 ttttcttgag catcatattt aaaagcatac caaaaccaat tgaaggtagt aaagcacctg
     6301 cagagttaag tccctgcata acgattggtg gaaccttagc aaacaagtct gccacatatt
     6361 cccctccaag cataatagct aggaaaattg gtacaatttt aaataacgtg attaaaacca
     6421 aacttgaata atgataaaaa ccgatctttg aataatctcc gtttttagca tcattatcag
     6481 cttttttcac aaaataaaca tctaaagttt ttgcaagaac atccaactgt tgggtagcaa
     6541 ctgcaattgg aactgctata gcgataccag cagtttctcc ttgaccagat aaaataccaa
     6601 atgcagtacc aataatcgca cctgaaatag tatctggtgg agtgtaacct ccaatacctg
     6661 ttacaccgag ccaagttaat tcaagagctg aaccaataag aacaccttgg gtgaaatctc
     6721 ctaagactaa gccaaccatt gcacttgtaa ctaacggacg gtgaatcata aataacggtc
     6781 cattatagtc aatagtggca ataatcccaa ctaatgtcac cagtaatgct tgtgtgaata
     6841 gcatatactg tacctcctta aattattttt ataaaaattt ttccaacatt gtaactttat
     6901 cattaggaac catttgaatt tcaactctcg ttcccttatc aactattttc ttaaaataat
     6961 atttgtcttc ttcacctaga gctacacctc ccgttacctt ttcattttcc tccgttttac
     7021 tcatctgtcc tacattgagg tactccaatt ttaaatttcc ttcaatagaa tcatacacat
     7081 cttttggatt tgtatatatc agcattgttc tcttttttat tggcacagag tttaaaactt
     7141 ccacaaaccg ttttacacta aagaaaacaa tttttaaacc taccggtgca gaaatcttta
     7201 aaatagattg tcgtgtttta tcttctgaga tgcgatcatt aacaatgata acttgctcaa
     7261 tatcatactt ttttagccac gtagtgacaa cttgaccatg taccagacgg tcatcaatac
     7321 gaacaaactc tatagacata aatccccttc ctcctctacg ttaagttgtt gtttaacatt
     7381 aaacaaacta ttttgagcat tttgaacaat ttcttctaaa ttgatttttg aatcataact
     7441 tgatattaat tccactagga gagggatatt aaaccctgtt acaatatcaa ctgaatccaa
     7501 atttaaaaac cgtgacaaag ccacattatt aggacttcct ccaatcaagt cagtcaaaac
     7561 gataacctct tgatttgagt ctaacagttc atccacttga tttttaaaat aatgttcaaa
     7621 ctctacaata ttctctccag gcatcaaacc taatgtccta actctatcat ttgttgtctc
     7681 accaacaaac atttctgcgg tcatgagagc tccgctagca aaattaccat gactggcaac
     7741 taaaattcct ctattaaaca ttttctcttt caacaactac tcctttctta aggataatat
     7801 tagcataaag tgaagtttct cctgtagcaa caatagcata agctttctta ctacgttcat
     7861 aaaagtcttc tcttctaaga taagtaatcg ttttaagatc tgtaccatga ccttcaatca
     7921 tctgtctata ggtaccccat atcttaggaa tatcatcacc cgaaacaacg ttcataaact
     7981 gaattgaact atcgacgtaa ctatctaatg gcattaaata cagaatggaa tctaataatt
     8041 ctggaatatt tacgccatca caacgaatta gcttatttgc acatgaggca gaaggataat
     8101 tcgcgtcagc taatactatt tcatctccat gtcccatttc cattaaagtc ttcaataaat
     8161 ctggagaaat atttttcggt atatgtttta acattaatat cctccctatt ttctctgacc
     8221 gtatgtttta aatttttctg ccattaaatc catctcctca tcaggaagaa ctactggctc
     8281 tccaaaattc ttagctaaac aataaatttt tgcacaatat tcaacttctt caacaatatt
     8341 aaatgcattc aataaatttt gtgcacctgc taaaattcca tgattcgcta gtaaaactgc
     8401 tctacgacct tccattgctt tagctgcatt cacagccaat tctttcgtac catatgttgc
     8461 atactcagct acccgaacat ctttccctgc cactgcaatc atataatgac tagccggaag
     8521 tggttctctg agacaagcta atactgttgc ataagttgta tgagcatgga taattgcatc
     8581 gatatcatca cgagtttgat attgaatcaa atgcatatac cattcgctag atggcaagcg
     8641 ttctccctct acaacatttc cattaatatc catcactaca atatcggatt ctttgatttc
     8701 aaagaaatca atacccgacg gggtaattgc catcaattgt ttttcacgat cgaaaacact
     8761 gagattccca cctgttcctt tcgtcaaatc tgtttctact agcttcttgc catacttaat
     8821 taattcttgt tttacatctg acattcttgt cctcctcata tcaaaacact aaatattttt
     8881 caattcacca attatccccc cttcttgtac aaaacttaag aagggggagg gaggcaaatt
     8941 tacaaactct caatcttttt aattcttttg ggagtaataa ctctctaatt gtagaaactc
     9001 ctcaataaca tggtgagctt cttccatccc ttttaattgt cccatagcta tgagctgcac
     9061 aacaatattc cccacagctg taccctcagt cgaacctgta agaacctctt taccagttct
     9121 atcagcaatc atttggttaa agtaactggc tctagcacct cctccaatca catatatcct
     9181 cttataaacc ttatgagtta gttcttctag taactctatc gctttcctat acgtttcagc
     9241 taggctttca taaacaatct taaatagttg tccatctgtc cactctctag tttcatgatg
     9301 ataagctaga tattctgtca aagtcttgtg catatcagat tctgttgcaa attcagttga
     9361 ttcagtatca atcagaggaa gattttcttt ttctttctcc accattgtcc taatatcatc
     9421 aaaagaatag gctttccctc ttcgttcaaa tgaacgtctt agttcctcta tgatccacaa
     9481 ccctgtacaa ttcttcagaa atgtaatcac tccatcttta ccgacttcat ttgtaaatcc
     9541 ataactgaag gattcggtag taagaatcgg tgaagtaagt tccactccaa ccaaagacca
     9601 agtacctgat gaaataaata aactaccttc tgtcttaggt actgagacaa tcgcacttgc
     9661 tgtatcatga ctacaaacat tcacaacagg aatatcgcct aaaccatact ccttttttat
     9721 ccttccaaga acatttccct ctgaaacaat ttcaggaagt aaagatgaat ccaattcaaa
     9781 tagttttaag atattctgat tccaattttg actcctagga tcaaataatt gagttgttga
     9841 agcaatgctt ttttctgtag caaacttacc tgtcaagaga taattaaaca aatctggcat
     9901 taaaagaatc ttattggtct tatagaaaga gtcaggagat tcttgacgtg ccttaaagag
     9961 ttgaaacaag gtatttatct ccataatctg atttcctgtc tctgaataca gtttttctaa
    10021 ttcagtcatt tcagatattt cctttaacac tccctttgtt ctttcatcac gataatgaac
    10081 aggttgtgat aacagcttac cttcattatc aatcagtcca aaatcaactc cccatgtgtc
    10141 aataccgata gataaaatct tgtaactagt attagccaag cggatacttt caagaatttt
    10201 agctagtaga aagtcaatat cccaagataa atgccctttt actctaatag gtagattaga
    10261 aaagcgattt atttcttcca ttactagttt attttcagaa aggtaaccaa cgattgctct
    10321 tccagaagtc gcacctaaat ctattgctaa aaccgcttcc atttttcttc tctcttcgtc
    10381 cttgattaag tttattataa tccccacatt ttattttttc aatatgtgaa cgcttacttg
    10441 gataatgttc aaatgttaac ccaatattca taaaaataat tcaaattcct ccaatttgtg
    10501 ctataatata gttgaaatga taaattagga ggagcttatg ataaaagatg aacgtgtact
    10561 tgaattgatt gaaattatta aaaagaaaaa aagaattgcc gtaaaagagc tggcagaaat
    10621 cactttctcc agcacaagta ccttacgtcg tgatttaatt ttcttagaaa atcaaggtct
    10681 tatcaaaaga aagcacggat acgtgaccct gtcctctatg aacacaattg aactttctca
    10741 tcaaatacgg gaaggagaaa gtactaggca aaaaagacta atcgctagtc tcgctaaaga
    10801 ctttattcgg tcaggtatgt gtgtttatct ggattccagt acgactgtct acgaactttg
    10861 tccctatctt tctgaacttg ataatttgat tatttttaca aatggtttac atactgcaca
    10921 aaccctatct gaaactgtta aagatagctc caaaatcttt atcacatctg gcgaggtcaa
    10981 acatcaatcc tgttccgtgg tcaattacga taaggaaaat tctttattag atcattttaa
    11041 tatcgattta gcattttgtt cagcaagagg tattgatgac caatatgttt atgaagcttc
    11101 tctcagccaa gctatttcaa aaaagaatat tattgacaaa gcccatgaaa ccatcttact
    11161 gattgatagc tctaaatttt acaagactgg attttttaaa attaatcccc tatccaaata
    11221 cacaaccttt atttctgaca ccgtgccaga tcaaaaatta ttagacgcag tcgaattatt
    11281 tgatggagaa tgggtttctg atattcaatg aaaatcaaat aggaagctag tctcagcttg
    11341 ctcaaagtac agatttgaaa actgatgaag tcaagtcccc catggacacg gcaagacgaa
    11401 gctgacatga tgtgaagaga ttttcgaaga atactcgttc tgtctgctga ctgatttaca
    11461 aatattcacc acatgacatt attttcttag caaccctaaa aacagaggct ggacaaaaac
    11521 tcaatttcaa gagaaatggg gcaaatattc ccacaataaa acgcatagta tcaagttttt
    11581 gcacacctga tattatgcgt ttttctgatt ttaaagactt tgtcgcctag accctatatt
    11641 tcaagaatta agtgaccttg atgatctaaa tgtattgtgg tatactattt ttgtcatcgg
    11701 ttaccgatta cgttcctatg ggttcgtgag aggaggtgaa actaatgagc cttatcatag
    11761 agctcgctct tactattata gcggatgtta tagctggaat tatcttgtat ttcatctgca
    11821 gatggctaga tagtaaggag tagaccgaat gactagccta taaacacccg ttaaatctct
    11881 aagatacgtc aaaaaagccc ttaactacgg ccatagttag gggctttggt gttctaatga
    11941 accttatcaa ttaactacat tttagcatat aatctacaat agttcaagca ttaagtgagg
    12001 ttgatgatct aaatttagtg tggtatacta tttttgtcat cggttaccga ttacgttcct
    12061 atgggttcgt gagaggaggt gaaactaatg agccttatac cagagcttgc tcttactatt
    12121 atagcagatg ttatagctgg aattatcttg tatttcgtct gcaaatggct agatggtaag
    12181 aagtagaccg actgactagc ctataaacac ccgttaaatc actaagatac gtcaaaaaag
    12241 cccttaacta tggcactagt taggggcttt ggtgttctaa tgaaccttat acactaacta
    12301 cattctagca tataagtcct aatatttcaa gagttttatt tattttttca ggttccctta
    12361 gttctgaaag gtctataatg aagttagcca tctagtatca aaaaaccgac tagctcttat
    12421 gaactagtcg atttctcatc aatgcgccaa catttcttgg gcgatttctt ggccagatag
    12481 gttatctggg tagtaggttg gccagttgtc catttcttca aagagggctt cttggcttgt
    12541 gcctccaaag aagatatgga aatgttctgc cttaactggg gcgatattgt ggtcactaaa
    12601 ctgaacatac ttgaattgtc cagcgtcagc atctgtggct tcaaagagga aacgcacgcc
    12661 acgattgcct ttcttgtaag tcaaaatttt cttaccgaca tacttgtaag tgtatttctt
    12721 gctttgtcca ccttgaacaa attccatagt attatcagta atgttaatct tagtcacatc
    12781 tgtctgatag ccttttgtat agtaagcctt gtactcagcc tgggtcatct taccagtcaa
    12841 cttagccttg tagtcaaaga cttggtcaaa cgtgccgtct tcaaggaaag gataaactga
    12901 ttgccagtta cctgcatagt cactcaaggt gcggtccttg acagctgcat cctcgaagta
    12961 accattttgg actgtcttgg tatcctctgc cttttcaggc tcgattgctg ggccttcttg
    13021 gtctgttgtt tgtttcaaag ccttgaggtt tttctccatg atagagatgt aattttctcc
    13081 agccttggtg tcctcttctg tcagactttc taaaggattg aggacatcag ttttgacacc
    13141 tgcttctttt gaaagtgtgt tagcaagggc ttgtgaggca ttttcttcaa aatagatata
    13201 ggcgatttta tttttcttga catactctgt caattctgcc aagcgagcag ctgatggctc
    13261 tgcatctgga gaaagtcctg agattgcgac ttgtttgagt ccatagtcca aggcaagata
    13321 gttaaaggct gcgtgttgag tcacaaagct cttttgtttt gcttgagaca agccttctac
    13381 gtaagcctta tccaaggctt gcaatttttc gatataggca gctgcattct tctcaaaggt
    13441 ctctttttta tcaggataat ctgctgacaa gctgtcgcgg atgtgctcta ctagtttaat
    13501 ggcacgaact ggtgataacc aaacatgggg gtcaaactca tggtgatgac cttcttctcc
    13561 atggtcatgg tccccctctt cttcctcgcc acctggcaag agcaacatat cgcctgtcgc
    13621 cttgatggtt ttcacttttt tcttatccaa ggtatctagc aatttaggta cccatgtttc
    13681 catgttttca ttttcataaa cgaaggtatc tgcatcttgg attttggtaa ctgccttggc
    13741 agatggttcg tattcatgag gttctgtccc agcaccgatt aggagttcta cattagccgt
    13801 atctcctgcg acttgcttgg taaattcata gacagggtaa aaggttgtca cgatattgag
    13861 tttaccatct gcctgttttt gattggaaca agccactaaa aacaaggcac atagactggc
    13921 tagtaataag ctaatttttt tcacgttcgt ctcctatttg ataaaacgtc ttactaaact
    13981 gattagtata aagacagtta caaaaataat ggtaatactt gcacttgcag gtgtttctgc
    14041 atagtaggaa atgtaaagtc ctgctaccat tcccaaaaag ccaatcgcac tggcaagcag
    14101 cataaccgat ttaaagtttt tccccagacg cagggcaata ctagctggca agaccataat
    14161 ggtcgatacc agaagagctc ctgctgcagg aatcataagg gcaatagcca cccctgtcac
    14221 catgttaaaa agaatggaca tggtacgaac tggcaagcca tccacaaagg ccgtatcttc
    14281 atcaaaagtt aagatataca taggacgaag aaagagaaag gtcaaaatca aaacaaccgc
    14341 cgcaatgaca aagagggaaa tgacctgttc ttcactgata gtcacgatcg aaccaaagag
    14401 atattggtcc aaactcattg aactcgagct tttacccttg ctcatgacaa tcagagaaac
    14461 agccagacct gttgacatga ggatagctgt cccgatttcc ataaagctct tgtaaaccgt
    14521 acggagatac tccagaaaga ccgccgcaat caagacaatg gcaatagtag aaacagttgg
    14581 agaaatcccc aaaaccagac caaaggctac acctgaaagt gagacgtggc taagggtatc
    14641 actcatcaaa ctctgacgac gcaagatgag gaaggttccc aataccggtg agaaaagact
    14701 catagcaata accgccaaaa aggcgcgttg tataaagtcg taagataata aactaagcat
    14761 ggctcacctc ctggccattc tcatgaacgt tgaaacaacg ccatggcgag tcttggttac
    14821 ggactagatg aatattgcga tccgcataat ccttaacttc ttcagggtca tgggtaatca
    14881 tcaaaacagc cttgccatga tgatgggcgc tgtggtgcat gagttcgtaa aattcatttt
    14941 tacttcctgc atccatcccc gttgtcggct cgtctaggat aaacacatca gggtcagaag
    15001 caaacatacg cgcaattacc gctcgctgct tttgtccccc agatagagac cccaagcgtt
    15061 tgtctcgatg ttcccacatg ccaactgagt ccagactggc cttgatatgc tcctcatcat
    15121 gagcattcaa acgacggaac cagccttttc tcggatagcg acccgacttg acaaattcat
    15181 agaccgtact tggaaaacca gcattaaaac tggcaatttg ttgaggaaga taggctattc
    15241 tcaatttctt accttgcgta tttgtctttg aaatagccac ctttccaatg cgtggttgca
    15301 gaattccaag actggccttg atgagcgtcg tcttagccgc tccattttcc ccagtcaagg
    15361 taacaaattc cccactatca acactataat tgatatgttc aagaacaggc tccttatcat
    15421 aatagaagga caaatcctct accgtaatat atctcattat ttgatttctc ctactaaagc
    15481 agtcaaaaac cgctgaatca ctttttgttc atttggagta aactgagtcg ccacttgttc
    15541 ataggttaaa agtgtatgct catggtgatg gtggtgctcc tcagcgattg gacgagccaa
    15601 gtcagtcaac tgataaaaaa tcacacgcgc atctttagaa tctttagatg tttccaacat
    15661 cccttccttg accaaagact taatggcctt ggtaactgcc gcctgactga cattgagacg
    15721 acgggccaat tctgaatttg ttaaagattc ctctgacaag agcataagga tatgctcctg
    15781 agtattggtc agggccacct cgctagtgca atgacctatt aggatttcat gctgattttc
    15841 cgcctgcaaa atcacctcat tcaaaaaagc attgatatcc tttgctagct gtctcatatc
    15901 tgactccttt ccttttagac ttctcttttt taagagaaaa atactattct ttgacatttt
    15961 gtttaccagt taattatatc acaagcaaaa aaagagtcaa gaaaaaacgt gaaaactagt
    16021 ttcattcttg aactcttcta tattatatta tctattgaaa ttctttgaca tctccatcat
    16081 aagtcgccca atctttgctg aaaaagcgct cattcagatg gtaagtcgga gctggtgtgg
    16141 gattggatag gaaaggatca actgccttgt caaaagccaa ccaacccaac caaccaaggt
    16201 gaatggtgtc cttcataaag aaaggctccc caccgtcctt agaaaaatct gctatattgg
    16261 taaaaccttg actttctaac tggtagcgaa tcttctgcac cgtttgttgg tacatatcct
    16321 ctcgtagacc agcatagtcc atccattttt tattaacagg tggaatgata aaaatcgggt
    16381 ttaccttaga tttagaaaac tgtgttaaaa ccaactgcaa gtcattatac tctggcgact
    16441 tgagataggt aaagcttttc tgagaatcct ttaatttctt caaatccttc ttgatctgct
    16501 cattatagaa ataattttcc attcccatct cattattgga agtatttttt tcagcatctg
    16561 ctttgacaac atcttctatt gcttgataag aaaactggtc tggcaagatt tttaaatact
    16621 tagctacatg cttatcgtag ttaacatagc ctctaaccga aaactgacca aaaaaggagg
    16681 cttggcgttc attaaaacga gccaataatt caatcatttc attgtctgct gtcgacaatt
    16741 ctcctttact tgccaacttc tgaaccaggt ccttcatagc tacgtttggg aactgttgca
    16801 gtaagcgagt cgctgcatat tgactagcct gatccccaga ttgatgtttc agaaaactag
    16861 tcaactggtc tccattaaaa tactgctgga aggctgctgg atcatagcca tttttactga
    16921 accactgagg tgagataaca tacacaactt gtttattctc cagctgtggt aacatctgtt
    16981 gcattccaaa atattggtta agcgatgcag ctcccccctg tcctaaaaga taaggacggt
    17041 aggaacgatt gtatttctca gctaataccg caggatgagc accgtcaaaa cgaagccatt
    17101 cactagagcc aaagaaggga acaaaacgca catttggatc agatagtgct ctgacttttt
    17161 gacttcgctc cttaaaacta tcgatagtag tagccactgc tgaacgcttt tcagctccta
    17221 gattatgatg catctcagta ggataaaaga aaatgagcag aaaaaccaac aaaccagcga
    17281 tcaagaccgg tccgaagatc atccataagc gtttaagcat tttgtagctc cacaatacca
    17341 gctatgattt tattagctgt attccagtcg tcacgaccaa actctgttac agggacacga
    17401 atgtcaaaac ggttctcaat ctccacaatc aactcaaccg ttcccatact atccaagaca
    17461 cctgcatcaa aaagatcttc atccatcatg tcagaaacat cttccataaa caactcatca
    17521 ataatttcaa taacttctga tttgatatcc atattttatt tccttttatt ttttaaacca
    17581 tagattattc aagaatccag aaaagattaa gaatgacaac atgacaacat ggaaagtgac
    17641 aaccatgcca agcaactgaa tccagcgatt ctcaggtagg gcagccttcc ctgctttttt
    17701 ccgttcctta ttgagcgttt ttttcttgcg aacccaggca tcattgatga ccaagcctag
    17761 tccatgaaag agtccatagg cgatatagta ccaggtcaca ccatgccaaa atcccataat
    17821 cagcatattt acaatgtagg ccatgcttga ggttacatta cgatttttaa agactttctt
    17881 tctggttaac accatcacca ttcgcataaa gacaaagtca cggaaccaga aggacagact
    17941 catatgccag cgattccaaa actcctttaa atcccttgat aaaaagggct tgttaaagtt
    18001 gatagggcta cggattccca tcaagtttga gatggccaaa gcaaacatag aataacctgc
    18061 aaagtcaaag aagagctcca gaccaaaagt atacataact gccaaggcat agagattaaa
    18121 gaagccacct gactgcaagg ctaaattctt cagaggaggt agtaaggtct ctcctaaaac
    18181 atgagctagg ataaacttat acaaaaagcc ccacatgata tagcggacag attcatccag
    18241 catatccatc aactcatctc gctcaggaat agcctgataa ttttcattaa atcgcttaaa
    18301 gcgatcgatt ggaccactcg agaaagttgg catgaagaga aggaaacgga ggaattccca
    18361 gagggtaaaa tccttaatca ctccatctct cagctcgatg ataatcccaa ccgaacgaaa
    18421 ggtcaggtaa gaaattccca agaacccaag caaagactgc gttccattga tagctggttg
    18481 caccttgaca aagataatcg gaagtaggga cagaaaacta actaagtaga agacccactt
    18541 gccatccttg ctttttcgat aatgcttgta gaaaagcagg agcaatattt cccagcaaag
    18601 gtaaataccc aaggcagcta gttgattggt ctttccaccc accaacatgg tgacaataaa
    18661 gaagagactt accaacactt cataccaggc aaagcgtttc ttgaaaaaga ggcctataaa
    18721 gatgggcaag gttgcagcaa tcacataaac aaaatactga ggattgccat atggctctaa
    18781 atgaggaagc tgttgaaaaa actccatcat ctcttattca cctcgttaat caatcctttg
    18841 atgtcaatct ttccatttgg agtcagtggc aaactgtctc ggtaaaggaa tttagatggc
    18901 atcatgtagg acatcatgat gtctgtcagg tcttccttga tggccttggt aatatcgata
    18961 tctcgctcaa actgctcacg aacactgtct tttaagatga cataagccaa tagattttgt
    19021 accttgtggt ccttgttata gcgcggtact gcgacagcag attcgataaa gcgagacttg
    19081 ttgaggtttt gagagacatc ttctaactca atgcggtaac cgttgaactt aatctggaag
    19141 tccatgcgtc cgccgtagag aagcaagccc tcatctgtta tggttcccac atcgcctgtg
    19201 tgataggctg gcagaccttc aaactcaaag aaggcttctg ctgttttttc aggattgttc
    19261 atataacctt ttgaaacagc tggcccagaa acaatgattt ctccctgttc gccatttggc
    19321 agtttatttc cttcctcgtc aatgataaag gttggagaat cagccttggt atagccgatt
    19381 ggtaggcgtt tgagagtcgc taacatctcg tctgtcacgg caactgctga cagagctact
    19441 gtcgcttctg ttgggccgta agcattgatg atacgggcat ttgggaaacg ctcgcgcagt
    19501 ttttgagctg ttttgaccgt caattcttca ccatcaaagt agaaatgcgt gattccaggc
    19561 attttttcac tgttgaagta ttcagacaac atggccatat ctgcaaagga tggtgttgat
    19621 gtccagatag cgattggcaa tgaaaagata gccgcaaaga gttgcttaaa gtcctgagtg
    19681 atgactgaag gaagagtgaa aagcgtacca ccaagtgcca aggtcggtgc ccaatacatg
    19741 acagacaagt caaaagaata aggtggctgt gccagcattt gcggacgact cggtgtcgca
    19801 aattccttat ccgtaatcat ccagtttgta aagctgagga gattatcatg tgaaatctgc
    19861 actcccttag gcttaccagt cgtaccagaa gtaaagataa tgtagtaatt atcatctccc
    19921 ttgactggat gcgtgatttc atagttgttc ccttgggcaa aggcttcttg aacctgagct
    19981 agatttatca ttggtgtaga aacctgctcc aagggaaagg ctgaaatggc aataatcaag
    20041 cttggctctg ctacttctaa aatagctgaa actcgctcca aggccgaatg gctatcaatt
    20101 ggaatgtagg catgacctga cttagtcagc gctacaaagg ttgccaacat ttcatattct
    20161 tggccaccaa aaacaaccac aggagacttc tcaggcaagc ctagttggtc aatgactgca
    20221 gccaaactat ccgaatcaga ctttaaatcg ccataagtgt gttcctgccc caaaacatta
    20281 tagacaggat agctaggctg tgtctgagca aaatgctcaa tggtttcaat catatctgct
    20341 attggtttat ttgacacaat agggattctc cttcaagtta aaattcatta tagataaagc
    20401 ttccttgacc ctgaccaaga tagctaaaga agtaaagcag ccctagaaag ataagaaaat
    20461 acaaggctgt ccgaccaaga aagaggtaca attcttttct ctgtttcatc aagaaaaacc
    20521 attcatttct gtaatttttc gctaaaataa gagtgattct tactagctta tttttctacc
    20581 attgtaccac tttatatagt atcttttcaa ttgtttaccg tatgtttcca atagatttca
    20641 gcttatttta aggattatgc ggtttttcta tatctatttc aaaagaaaaa agcctgagat
    20701 tttcatctca agctctagca ataatagtta ttttctaaac taaaaaagtg agaatacaag
    20761 cacggccaag gctgcaccga taacaggtcc cacaacagga atccaagcat aagaccagtc
    20821 tccgtctccc ttgtttgaaa ttggaaggat actgtgcatg atacgaggtc caaggtcacg
    20881 cgcaggattc aaggcataac ctgttgtccc acctagcgat agaccgatac cgacaatcaa
    20941 ggtacccact gcaaaggttc cgatacctgc ctgaaaatcg taaagaccca aagcaaagat
    21001 tgtcaacacc aaaacaaagg ttccaaggat ttcgctaatc aagtttgata cagtatcctt
    21061 gatggctggt ccagtactga aggttgccag gatattgcct gcattttctt ctgcctcata
    21121 gtgaggtttg aattgcaacc aaaccaaaat ctgacccagc atagcccctg cgaactgggc
    21181 taagatataa ggcaaaacgg aagcccaagg caagtcacct tttaaagcta caccaattgt
    21241 cacagctgga tttaaatgag ctggactgag cttgccagat acaaatactg caaccgcaac
    21301 tgcaatcccc caacccatag taatcacaat ccaacctgag ctattgctct tggttttagg
    21361 aagaaccaca cctgcaacaa caccatttcc tagaagaatc aggattaaag tccctagaaa
    21421 ttctccaaat aattcattca tcatctttct gtctccatta aaaagaaggg acgagcgaca
    21481 aggaatgctg ccctccacct cttttatttt ttcttaattt tttaattctg ctaaatcgtt
    21541 gttagcgaga gctgcttcga catcagcacg gtaagttgct ttttcttctt ctgtccagtc
    21601 atagaatcgt cccatttcat ccaaaactgg ctcaacgatg ctatccaagc tatcacgcat
    21661 aaagagcata tggttggtac gacgaagaag gaagtcaact gggctaagag ccaactcgtt
    21721 gcgcattgca tagtgaaggg acaaagtatc tgccaagctg agtcctggcg cttgttccaa
    21781 gctgtgagca agtgcaaaga ctttcggtgc atttgaaccg taaagatttg ccagatagtg
    21841 agcttccttg ctatccaaac cacgtgatac tccaagttgc gcaaaggctt cgatttctga
    21901 atccacattt gctgggttca attctccacc tgaaacaggg taagttttag aattgatcaa
    21961 tttaaagcta cggtcaaatt ctgctttgag gatgtcaacc acgcgctcca tagctccttc
    22021 agccatctta cggtagtctg tgattttacc gccagcaaga gtcaaaagac cattgtcatc
    22081 acggtccaag ctagacccac gagaaactgc agatggatcc aaatatttct cagatgtgct
    22141 actttcaagc ttgctgacag cagactcaac atcttcacgt gttttttctt tggagagata
    22201 agattcaaca gtcgcaatca agttgtcaaa gctttcatca ctgatggtac cgttatttcc
    22261 accattatag tcagaggcac tgttccctgc aatcaatgga cgaagacctg cccagctgct
    22321 ttcgatatca tcaatggtga tgttggattc tggaaagcgg ttgttgacaa tgccaagtag
    22381 ataatctaca tcttcttgag ttacttttgg atgctccaaa tcacctgtgt agtctgtatc
    22441 agttgtacca aagtaagtct tgttttcacg tgggagaaca aagaccatac gaccgtcacc
    22501 caaacctgtg tcgaagtaaa ctggctgtga aaccttgatt ttgcttgaat ccactaccaa
    22561 gtgaactccc ttagttgggc gcatttgtga gaattgcgtt cccttattag acaaattacg
    22621 tactttatca ctccaaggac ctgttgtatt aataaccaga cgggccttga tttcaaatac
    22681 ttggtctgtc aagagatcac gagctacaac acctgtaatc ttgccacttt cgtcaaagag
    22741 gaagccttct gccttcacgt ggttggcaat gagggcaccg tcttggttgg cacgtttgat
    22801 gttttcaatc acgagacgcg catcgttgtt acggaagtca agatacactc cacctcctac
    22861 caagccttcc ttcttcaagt ttggctggcg ttccaagact tgatccttgc tcaaaacctt
    22921 gttcgcagct gacgtgttgc tcacacctgc taaaaggtcg tacaagtcca tggctacttt
    22981 aagacggaag aggctaaagg ttgctccatc ttcatcgtaa actggtaaga gcattggatc
    23041 tgattttgga atgtgtggag cgatttgttg aaccactgca cgttcagaaa ccgtatctga
    23101 gaccacttct acgtcaaatt gtttgaggta acgaagtcct ccgtgaacca attttgttga
    23161 acgactagat gttccttctg caaagtcttg catttcaatc aaaccagtct caagaccgct
    23221 agctgccgcc tgcaaggcta caccagctcc tgtgattcct ccaccgataa tcaagaggtc
    23281 cagggtacgt tcctgcattt ttttaattga caattcacgt gttttctttg aaaattccat
    23341 atttacacac tttctaaata aatcgcttta ttctgccagt attagtcgtc tacttccgca
    23401 aagacttgag ttgctttcac agccttcttc cagcccttgt agagttgttc cttgcgagat
    23461 tcgttcatag atggctcaaa gagttctcct gtctcgttca agagtttcaa ctcgtccaag
    23521 tctttccagt accctactga caaacctgct aggaaggccg ctcctagagc tgttgtttcc
    23581 aggtttttag cacgtgcaat gtcaatgcct aaaatatccg cctggaactg catgaggaag
    23641 ttgttcatgg ctgcaccacc atccaccttc agtacttgaa tggcggtctg agtatccact
    23701 tgcatggtgt cgatgatatc acgcacttga taagcaatag attgcaaagt cgccttgata
    23761 aagtcttctt tgcttgttcc acgagtcaaa ccaaagacgg aaccacgagc attttggttc
    23821 cagtatggag cgcctagacc tgtaaaggct ggaacgacat aaacttcatc gttgttgtga
    23881 gaatcacgag cgtatttttc agattctggt gaattttcaa ccatgcgaag accgtcacga
    23941 agccactgaa tagcacttcc tgcgatgaag atagaacctt ccaaggcata ataaacctta
    24001 ccgttgattc cgtaaccaat ggttgtcaag aggttgtttt cagacaactg catctcttcc
    24061 ccagtattca tgatgatgaa agagcctgtt ccataagtat tcttaaccat acctggctca
    24121 aaagccaact gtccaaagag ggctgcttgt tggtccccag ccatacctga gattggcacc
    24181 tctccaccgt agaaatggaa tggagctgtc ttgccgtaga tttcggagtt agaacgaact
    24241 tctggaagta tagccttcgg aatgttaagg atttccaaaa tctcatcatc ccatttgagt
    24301 tctttaatgt tataaagcat ggtacgagct gcatttgagt agtcagtcac gtgagccgca
    24361 ccgtcagtca atttccaaac caaccaagta tcgatagtac caaagagcaa ttcccctttt
    24421 tctgctcgct cttgagcacc ttctacatga tccaaaatcc aacgaacctt ggtagcagag
    24481 aagtaagcat caataatcaa accagtcttt tcatggaatt tttccacata accttggctt
    24541 tttagttgct cagccaaagg tgctgtctgg cgtgactgcc aaacgatagc attgtagata
    24601 ggaagtcctg ttttcttatc ccagacaacc gttgtttcac gttggttggt aatcccgatt
    24661 gcctcgattt gatttggctt gacaccactt tcgatgaaag cacccgcaat aactgactga
    24721 acagagttcc aaatttcatt ggcattgtgc tcaacccaac ctgcctgagg gaaaatctgg
    24781 gtaaactctt tttgactcga gctaaccttt tccccttttt tgttgaaaat gatggcacga
    24841 gaacttgtag ttccctggtc aatggccatg atgtattttt cttgtgacat gtactgtcct
    24901 cctgataaag atcttgaggt gtttcctctt tacacttact agtatacaaa ataaagcgct
    24961 ttcttgttta tcaacttttt ttaattttta aaaaaatgtg aggagattgt gtgaatttca
    25021 caaaaaagac ctggattaac caagcctttt aagtgaataa taactggcga atcgctacca
    25081 aatcctccat atccaagtca ttttttaaat aatagactgg tgtctgttcc ttcttatgaa
    25141 tcgggttatt ggtaaccagc aaatcataat gccgactagg gtcataagct tcaatggtaa
    25201 taaaacgatt gtattccaaa taccgtttca aaatggctgc catccttgaa aagacaagaa
    25261 aaccagatgt caaatccaga ccaatccgca tatgctggcc accgttttca gccatatatt
    25321 cgattaactg gagccattcc cagagaatct tcttatctaa atctgtcccc tgttgaaaag
    25381 taggtagagc atgaaaaatc tcctctgctg tctctttaaa atcatgttcc atcagtaaat
    25441 atggatgacg attctctaac tggtacttgt agcgatcctg taaaatatag cccttataga
    25501 gatagacttg agcacaaagc tgactgagtt cataggtgac atggtcctct gtataatccc
    25561 ccaatagttc ctccttcttc atttcttgaa tcaattgcgt cagtaaatct gccaactgcc
    25621 ctccaaaacc aagaatatac tccatagtat gaagaggaag aatgcgatga gataggagaa
    25681 aagagaagaa tatcatcatc tcaccatcct caactcctag tggaatatgt tgcccagcaa
    25741 aaaaggacgg aacctttctc agcaaacgaa gataaaagat attgtcaaaa taccctcgca
    25801 ttttctcttc tatgacttga aactgacaag cattgacccg aagacgttgt tgactgatgt
    25861 gagcccagag aaccaagtcc aatttctgcc ccacagacaa acttgcaccg cagatttcct
    25921 ctaaattggc aatctcctgt tttctctctg gtttctgcat gtgaccttcc cattcctgac
    25981 tcgaccagac ctttcggaaa agacagaaat agaaatagcg aatctgatgc tctggacctc
    26041 gccaacgtcc attttggatg gataaatcaa attctgacag aatctgattt aaaccagcca
    26101 agtgacgacc aagcgtagcc tcgctaatca ccaattcttg agccagctga tgggctaaaa
    26161 actgttggtg gtagagaaga taaaccaaaa tctggtattt aacagcactc tccaaaaata
    26221 agctccgaat atctctcccc ttggtagctg caccgataga cagacgcaaa ttttcatttt
    26281 ctgagtgaat agtcagctct aagccactat ccaaagcctt gtcattgagc agggtgacat
    26341 atttggttag ggttgctttt gaaaatcctg tttcttccat tacagccttg acggtcgtct
    26401 gagactcttg taatagaaag gaaaggattg aaaattgacc acactcagct ttctccatca
    26461 aatctcctaa atacatttgt tacccctttc attttctacc ttaagcatag cataaatctt
    26521 acaaatgctg aaataatctg tttctctttt tattttcatg ctgatttcct ggtccattat
    26581 cctgaaaatc agtaaacaca cggctccccc tctgggcatt tttatgctaa aatagtagct
    26641 atgaataaaa ttattaaaac tatatcagaa agcggagcct ttcgtgcttt tgtccttgac
    26701 agcacggaaa ccgtccgcac tgctcaagaa aaacaccaaa cccaagctag ctcaactgta
    26761 gcgcttggtc gaactcttat cgctagccag attctcgcag ccaatgaaaa aggaaatacc
    26821 aaacttacag ttaaggtgtt gggatctagc tctctaggtg ctattatcac cgtcgctgat
    26881 accaagggga acgtcaaagg ctacgttcaa aatcctggtg ttgacatcaa aaagactgcg
    26941 actggtgaag tcctagtcgg accttttgtt ggaaatggtc aattcctcgt tatcacagac
    27001 tacggtactg gaaatcctta caactctata actcccctca tctccggaga aatcggtgaa
    27061 gaccttgcct tttaccttac tgaaagccaa caaacgcctt cagcggtcgg cctcaatgtc
    27121 cttttggacg aggaagacaa ggtcaaggtt gcaggtggtt tcctagttca agtcttgcca
    27181 ggagccaaga aagaagagat tgctcgcttt gaaaaacgca tccaagaaat gccagctatc
    27241 tctactcttc tcgaaagcga cgaccatatc gaagccctcc tcaaggctat ctacggggac
    27301 gaagcctaca agcgtctttc tgaagaagaa atccgtttcc aatgtgactg tagccatgaa
    27361 cgctttatga acgctcttgc cagccttcca agctcagact tacaggaaat gaaagaagaa
    27421 gaccacgggg cagaaatcac ttgtcaattc tgccaaacta cttacaactt tgatgaaaag
    27481 gacctggagg aactcattcg tgacaaatct taatacacct tttatgattg gcaatgttga
    27541 gattcccaat cgtaccgttt tagcgcctat ggctggcgtg accaactcag cctttcgtac
    27601 catcgcaaaa gagctcggag ctggactcgt tgtaatggaa atggtctctg acaaaggaat
    27661 ccaatacaac aacgaaaaaa ccctgcacat gcttcatatc gatgagggcg aaaaccctgt
    27721 ctctatccaa ctttttggta gcgatgaaga cagcctagca cgcgcagcag aattcatcca
    27781 agaaaacacc aagaccgata tcgtcgatat caacatgggc tgccctgtca acaaaatcgt
    27841 gaagaacgaa gctggagcta tgtggctcaa ggatcctgac aagatctact ctatcatcaa
    27901 caaggttcag tctgtccttg atatccccct tactgtcaaa atgcgtaccg gatgggcgga
    27961 cccatctctg gcagtagaaa atgccctcgc tgctgaagct gcaggtgttt ctgccctcgc
    28021 catgcatggc cgtacccgtg aacaaatgta tactggccac gcagaccttg agacccttta
    28081 caaggttgcc caagctctaa ccaagattcc attcatcgcc aacggtgata tccgtactgt
    28141 ccaagaagcc aagcaacgca tcgaagaagt tggtgctgac gcagtcatga ttggccgagc
    28201 tgccatggga aatccttacc tcttcaacca aatcaaccat tactttgaaa caggagaaat
    28261 cctacctgat ttgacctttg aagacaagat gaagatcgcc tacgaacact tgaaacgatt
    28321 gattaacctc aaaggagaaa acgtcgccgt tcgtgaattc cgcggcctcg ctcctcacta
    28381 tctccgtgga acatctggcg ctgccaaact ccgtggagcc atttcacaag ctagcaccct
    28441 ggcagagatt gaagctctct tgcaattgga gaaggcttaa tagtttaaaa cccgtaactc
    28501 tcttaaagag tctcttgagt gccgccattc tttcctcatc aaaaaaactc cgtagtctct
    28561 agcgccataa atccggctag agattacaga gttatttttt aatctaaact tcctacaagg
    28621 gtatagtatc tactgaaagg atatggtatt tgtagggaat actttaaaga gttcttacag
    28681 gaagccagca ttaacgagtt acattaggtt tagtttaccc atttaccatt ggcattgact
    28741 ctatagccac ctacagttgt gttgactgca agggcacctg agccattgac atagtaccat
    28801 ttgtctgata ctttgaacca ttggctttct ttcatagccc ctgatgcttc aagatagtac
    28861 catgtatctc catctttcag ccaacctgtt gccatcgaac cgttagcatt
//
LOCUS       DAVLXF010000017        28364 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLXF010000017 DAVLXF010000000
VERSION     DAVLXF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.017"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            180..416
                     /locus_tag="VYO12_000686"
     CDS             180..416
                     /locus_tag="VYO12_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV1587334.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRKDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            647..1933
                     /locus_tag="VYO12_000687"
     CDS             647..1933
                     /locus_tag="VYO12_000687"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1587335.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(2175..3323)
                     /locus_tag="VYO12_000688"
     CDS             complement(2175..3323)
                     /locus_tag="VYO12_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1587336.1"
                     /translation="MKYNKTKYPNIFYYETAKGKRYYVRRSFFFRGKKREKSKSGLTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSTLLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(3694..4101)
                     /locus_tag="VYO12_000689"
     CDS             complement(3694..4101)
                     /locus_tag="VYO12_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEV1587337.1"
                     /translation="MALFGKKQDESLEVELFTEEPNERVFEFKKSKTVVRIDDYFIRI
                     ARKSNVSNVLLHGLDGEKSILLSEITAYQLKEPGSTVGYLQLVYPGSSDTKGGVFDAV
                     KDENTVTFTKDEKASILELKKAIEKALKDKVKK"
     gene            complement(4146..4529)
                     /locus_tag="VYO12_000690"
     CDS             complement(4146..4529)
                     /locus_tag="VYO12_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1587338.1"
                     /translation="MTEKEIISHFQVRIVDFDGELIPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDALESLDDPKEFDYLK
                     FMSYYNLKTVTNEVMVKEEYQTLIG"
     gene            complement(4542..4907)
                     /locus_tag="VYO12_000691"
     CDS             complement(4542..4907)
                     /locus_tag="VYO12_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1587339.1"
                     /translation="MFLAFDRIKELADKQKISLNILEEKLGYSTNYLYSLKKGNPKSD
                     RLQEIADYFGVSTDYLLGRTENPNLADDTKEYIWQGKVLNVEEMASNVMMFGGRELTD
                     EKKKIIQSIIEGYLKEAGD"
     gene            5194..5403
                     /locus_tag="VYO12_000692"
     CDS             5194..5403
                     /locus_tag="VYO12_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1587340.1"
                     /translation="MSQQHKKWIRLVKDKLNSEGMTQTHLARACGVKKSTISELLKYG
                     KGSDKLKNRVCDVLRIDETWVELGE"
     gene            5407..6117
                     /locus_tag="VYO12_000693"
     CDS             5407..6117
                     /locus_tag="VYO12_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEV1587341.1"
                     /translation="MNEIFNFHGQEVRTLTIDNEPWFVGKDVADILGYSKARNAIALH
                     VDEEDALKQGIPTSGGTQDMLIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRKQ
                     GGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAQSLLKKRKAR
                     VVACLGGIDSPAYADKIFAQSVFRQAEIDFKDHFNISRYDLLPKKFAEAALAYWMTWE
                     PSTNTKMKIIKLNSFDEG"
     gene            6130..6387
                     /locus_tag="VYO12_000694"
     CDS             6130..6387
                     /locus_tag="VYO12_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1587342.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCD
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            6473..6793
                     /locus_tag="VYO12_000695"
     CDS             6473..6793
                     /locus_tag="VYO12_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587343.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEKRKEEERREEQEF
                     ASMIIQGYNHAYERGREAERQEIRKNCRREFKGFTYDNEPPVDLRPEPLALPEPKQSA
                     IRFL"
     gene            6809..7105
                     /locus_tag="VYO12_000696"
     CDS             6809..7105
                     /locus_tag="VYO12_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587344.1"
                     /translation="MQELIESLDNLIMIVKELEGRESTSRHFITIWENDYKNLLLVKE
                     YLTDEKLAKDYRYMTLKNKLLKIEKMELEGRYILYEDMRMKYRANRRKWGARYV"
     gene            7098..7925
                     /locus_tag="VYO12_000697"
     CDS             7098..7925
                     /locus_tag="VYO12_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1587345.1"
                     /translation="MSEIKWIKIKTDIFDDEKMCLIDASPDRDAIIVIWIKLITLAGK
                     LNTKGVLTISKNIVYTDEMLAQTFHRPLNTVRMALEVFEKFGMVEKIDGVIMLPNWEK
                     HQNIDGMEKIKEQNRNRAARHRQKQKLLAQNNESNVTDNVMDNVTVTHGNALDKELDK
                     DIEINNNKVMISSSLSENLKNSGIHLTDKSHQQLLDYVGLDGMSFDMLNRAVEKTSGS
                     HKPSFNYLIAILESWKKKGFTSIEQVDEDDRKYKEGKKSRQQNDKTSEQEVRDEWGF"
     gene            8065..8835
                     /locus_tag="VYO12_000698"
     CDS             8065..8835
                     /locus_tag="VYO12_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1587346.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQERVNELANAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LAFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIANAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLTS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            8850..9044
                     /locus_tag="VYO12_000699"
     CDS             8850..9044
                     /locus_tag="VYO12_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587347.1"
                     /translation="MFILRHGTREDKPFLKSVVIGVTGLDISCSEEKKAMRFVSRGAA
                     VQVGKALRGSFGNFYPVEVE"
     gene            9044..9262
                     /locus_tag="VYO12_000700"
     CDS             9044..9262
                     /locus_tag="VYO12_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587348.1"
                     /translation="MLELYFVYNGHCKFYLGTFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGE"
     gene            9262..9567
                     /locus_tag="VYO12_000701"
     CDS             9262..9567
                     /locus_tag="VYO12_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587349.1"
                     /translation="MAKNILMDLAFENLHKCMGISDWKESDEVILVSSANKEQIESDE
                     SYHSAGKCNYLGKRICIFCEQVKKNNYITLHKSMLEKIIKTMESFTDAEQMKGEEND"
     gene            9644..9742
                     /locus_tag="VYO12_000702"
                     /pseudo
     CDS             9644..9742
                     /locus_tag="VYO12_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            9756..9923
                     /locus_tag="VYO12_000703"
     CDS             9756..9923
                     /locus_tag="VYO12_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587350.1"
                     /translation="MVGVTYKEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            10094..10408
                     /locus_tag="VYO12_000704"
     CDS             10094..10408
                     /locus_tag="VYO12_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587351.1"
                     /translation="MEELKQKVNEVYNWTVEDGKPQPPKQDLPQVVKDRADYFWEMAE
                     DGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWIGYSPSMAQVVIAVYLIYG
                     GN"
     gene            10410..10919
                     /locus_tag="VYO12_000705"
     CDS             10410..10919
                     /locus_tag="VYO12_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1587352.1"
                     /translation="MNKQELIKKLEERRTITGNFQGYVVWWKDVKEIFEQLGEPQPVK
                     VPQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLNGYEVEK
                     EKRYLVKVKGVHFTNYLISGNRKDIWFFHSAYEPEHQIIAHTRKELEEAGFGWVFDCP
                     GIEIEEVER"
     gene            10916..11203
                     /locus_tag="VYO12_000706"
     CDS             10916..11203
                     /locus_tag="VYO12_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587353.1"
                     /translation="MTQTLEEGMKNQSKCIKVPREIRPFDIGHRIVNKDGQALALRNG
                     ASIFDLPFLAEKAIEKEFGKNDPDFDIGKHSVEEVAIVNLSKFHSYFEGVE"
     gene            11203..11391
                     /locus_tag="VYO12_000707"
     CDS             11203..11391
                     /locus_tag="VYO12_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HEV1587354.1"
                     /translation="MSCSENLKKEKELTAAISNFKIEVLQNDDKLSSLSLSNIKRQAR
                     DLYECLVWLQYNAEESGR"
     gene            11388..11726
                     /locus_tag="VYO12_000708"
     CDS             11388..11726
                     /locus_tag="VYO12_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587355.1"
                     /translation="MSYDLEILVKIESGDYICIAEPKYSSPIYNLGRMFRVAMNWDFD
                     QDTTYNIADVLDNIQRGISELERYPEKYVQYEPENRWGTVSVALEVLKSLKECILEQD
                     IDTKYLYMRW"
     gene            11728..11988
                     /locus_tag="VYO12_000709"
     CDS             11728..11988
                     /locus_tag="VYO12_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEV1587356.1"
                     /translation="MRRFIAIWILVSAGLNIWHMDRIRDLEEKKPMVVYKADNAGAEI
                     FGRVVEKGRHGKLYTVTIRDYGIFVVTKEQFEKIRVGDEVLL"
     gene            11988..12446
                     /locus_tag="VYO12_000710"
     CDS             11988..12446
                     /locus_tag="VYO12_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587357.1"
                     /translation="MDDILQALAKMLNMTVDEVSSLLTTFKGNAPQIYEMFVKEKMFY
                     DLFSLFQIMSIVIFSISAVVLAVLTLIYFTYDGGFVYSYDIRTGKTEEEIKLERIERK
                     RKDLKIPLKISCISSSASLITVVIAIVLKATLAPNYIFIVNEILPKLTKR"
     gene            12455..12524
                     /locus_tag="VYO12_000711"
                     /pseudo
     CDS             12455..12524
                     /locus_tag="VYO12_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093849.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            12558..12908
                     /locus_tag="VYO12_000712"
     CDS             12558..12908
                     /locus_tag="VYO12_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587358.1"
                     /translation="MILKFRVWHHELGRLMSVKCMFFQDSEIEEFELNDTLMNDYITA
                     YPDEIELMQSTGILDKNGKEVFEGDIVRFFDSLYTVFYDIKDRWVVDYMSNFSSEESI
                     EIIGNIYENLVYIF"
     gene            12926..13327
                     /locus_tag="VYO12_000713"
     CDS             12926..13327
                     /locus_tag="VYO12_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1587359.1"
                     /translation="MKKLSDEDLKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSSP
                     SVGISKPTETIAVRIADDPTLKFLEGFKAIINKLLINLVDEDKEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAILKGEL"
     gene            13518..14060
                     /locus_tag="VYO12_000714"
     CDS             13518..14060
                     /locus_tag="VYO12_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1587360.1"
                     /translation="MEFVSPIKDNDDIQAMKDYLREWNEMYYMLFITGLNTGLRVGDI
                     LTLKVKDVQGWHIKLRERKTGKQITRRMTKELKKEMRRYVEGKPFHHFLFKSRQGQNK
                     AITRERAYQIIHEAAEELGIDNVGTHTMRKTFGYKYYNKTKDVGTLQKMFNHSSPAIT
                     LRYIGIEQAELDDALRNFVI"
     gene            14225..14416
                     /locus_tag="VYO12_000715"
     CDS             14225..14416
                     /locus_tag="VYO12_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587361.1"
                     /translation="MVKEYRDDFLGEKAFEKLNKDIDANPEVGFEIVGYTQTAFVNGM
                     HIPLTAILVKWNNFFKESE"
     gene            14536..14856
                     /locus_tag="VYO12_000716"
     CDS             14536..14856
                     /locus_tag="VYO12_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1587362.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLNFF
                     KNEK"
     gene            14994..15386
                     /locus_tag="VYO12_000717"
     CDS             14994..15386
                     /locus_tag="VYO12_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEV1587363.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYADMGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            15379..17109
                     /locus_tag="VYO12_000718"
     CDS             15379..17109
                     /locus_tag="VYO12_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEV1587364.1"
                     /translation="MGNLDKAKKYAQHVLTHREEHCEENILAAERFFRDLENPTFEMD
                     EDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVNYLDENILKMANPSWGVTVSLKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYH
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     KMREKGFKIAAVGMDRKFGREFLTKMKQARFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFACIQALANLG
                     KGGDVMRFFD"
     gene            17117..17335
                     /locus_tag="VYO12_000719"
     CDS             17117..17335
                     /locus_tag="VYO12_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587365.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNGEVVTIRQRHMKNLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            17353..18555
                     /locus_tag="VYO12_000720"
     CDS             17353..18555
                     /locus_tag="VYO12_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1587366.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCKHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDEVVHFV
                     INPDPDIPYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            18539..19114
                     /locus_tag="VYO12_000721"
     CDS             18539..19114
                     /locus_tag="VYO12_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEV1587367.1"
                     /translation="MQKRNSYRATQFQTREEESGDLILSGYFIKFDEETELWPGYCEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFNL"
     gene            19111..20283
                     /locus_tag="VYO12_000722"
     CDS             19111..20283
                     /locus_tag="VYO12_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEV1587368.1"
                     /translation="MNKLLILGARMRNKADKVVELEESIKELNKRSELEAKKLEQAGT
                     DEEVSAVEKNLEDIQKESDEKEAEKEQLEKEIEDLKNQVEELNRKAPTYPSQEKRGGQ
                     KLEQRDAIAKYIRTGQTRDIVGLKTTDSGSAALIPTEVLKPHFVNKTRNPLLDLVERV
                     KVNSGSGKYPVIKKTDGVMVSIDELKSNPELGKPAISEIDYSIKTYRGYVPVSQEMID
                     DADYDIMSIVEDEVFNQGDNTELSLVTAVLKTATQADAAGFDGIKDIYNKKLKSIYKA
                     SIVVTKSMFAALDKVKDKDGRYMLQTDVASPTGYSFGGKTIYKVDDTVFGNEGDMKFF
                     IGDVTEFVKEFDRSQVSVKWVNNDIYGQLLGLFIRLDIKKADEEAGFFGTYTDVVA"
     gene            20295..20585
                     /locus_tag="VYO12_000723"
     CDS             20295..20585
                     /locus_tag="VYO12_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587369.1"
                     /translation="MSYKVIRPFKDLADPENHDYAVGDIFPREGYEPTDSFTNGLLTG
                     ANTAGSIFLEVLGDDEPKKPAPETKEVKEEPAVEQEETVEETAEESAEEVEE"
     gene            20588..20869
                     /locus_tag="VYO12_000724"
     CDS             20588..20869
                     /locus_tag="VYO12_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEV1587370.1"
                     /translation="MDQGQLLELLKLKLGISTDLRDKPLKKIISSVITELTDNLGIEL
                     VGERADHEMFIVDYAAYRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            20856..21155
                     /locus_tag="VYO12_000725"
     CDS             20856..21155
                     /locus_tag="VYO12_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEV1587371.1"
                     /translation="MWNHEITLISKKVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEVLK"
     gene            21152..21499
                     /locus_tag="VYO12_000726"
     CDS             21152..21499
                     /locus_tag="VYO12_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1587372.1"
                     /translation="MSNDLADLIAKELAAYSDEVTEEVDKIAEQVTDETVDELKETSP
                     KRYGKYRRSWKKKKLANGSFVVFNAVASLTHILENGHLSRNGGRVAGIVHIKPAEEKA
                     IQNFEKRIKEIGK"
     gene            21496..21819
                     /locus_tag="VYO12_000727"
     CDS             21496..21819
                     /locus_tag="VYO12_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587373.1"
                     /translation="MKLSDFAAILEQANLPVTYRAFKIGNAPDLPYLVYYESSPVINS
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWANHKLFFEVQEETFIETERLYVKSY
                     TVYLY"
     gene            21831..22409
                     /locus_tag="VYO12_000728"
     CDS             21831..22409
                     /locus_tag="VYO12_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1587374.1"
                     /translation="MTQENKVTFGLENVHIAPIKTLAADGVITYGDVFRFPGAIELIL
                     DTKGETTPIKADNKDYHFMNSNEGYEGKLKIPHIIDEFATKILGEIKDPQTGVMTEKA
                     DASLTEFAMMFQFEGDKNKTRYVMYYCFASRPSLGSKTKNGTSTNERELSFKASPRPL
                     DTVVKRSITSADDKDAYDNWFKKVYEPTAVAA"
     gene            22421..22840
                     /locus_tag="VYO12_000729"
     CDS             22421..22840
                     /locus_tag="VYO12_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587375.1"
                     /translation="MRKIVLVGDQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETDKELTATNIDISMLSDFDMTFFNRLFWTFAKSANPHIKPYEQFFMEME
                     IFPIQEVGPVLMEMLNASVTTKKHQMSQNQLAKKSSQ"
     gene            23118..26204
                     /locus_tag="VYO12_000730"
     CDS             23118..26204
                     /locus_tag="VYO12_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEV1587376.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKAINKESVNTTNELKQIDKAL
                     KFDTGNVILLTQKQEVLQKQIGITRDKLETLRQAQSKVDEEFKKGNIGSEQYRAFQRE
                     VEVTQNVLKGYEGKLASVTQALEGNGDAAKNNQAQLKELQNEQKLLASESEKVVSSFK
                     LQESQMGANASEADKLALAEKKIGAQSEIVTRQIENLEKQLSLTKEQYGENSAEANKM
                     EAELNQAKTAYANLNQELGKLGSTAKSNQTQLKELQNEQSQLASEMSKVTSSFKLQES
                     ALGSNASEAERNALAQKKIGAQSEIVSKQISNLEQQLEITKKEFGENSTQANKMESEL
                     NQAKTAFNHLNDEMKVTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKLVEVG
                     KEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMASFAK
                     TSGLDTAEALDLTSRATRAAADGAAFYDKSIESVTESLQSFLKGNFANDAALGISATE
                     TTRNAAANKLYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENVMGNL
                     KQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFSSLPAPVKDFIVILGVVLTIV
                     GALAPIFLTLQAVFMSSFGAMIAAALPIIGIISGVVVAIAAIVAIVKYLWETNEGFRD
                     AVTTVWNAILEVINAVVSEISNFVMSIFGTVVTWWTENQELIRTSAETVWNAIYTVIS
                     TILDILGPLLQAGWDNIQLIITTTWEIIKIVVETAINVVLGVIQAVMQIITGDWSGAW
                     ETIKGVFSTVWQAIQSIVQTIFSAIQSYISNILNGISGTVSNIWNSIKDTVSNVLNAI
                     SSTVSSVWEGIKSTISSAINGARDAVSSAIEAIKGLFNFNISWPHIPLPHFYVSGSAN
                     PLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNNIMVGGEAGNEAVLPLNDKTLGAI
                     GRGIAQTMGGTSPTINITITGNTVREEADISRIADEVAQRIADELQRRRQLRGGFA"
     gene            26201..26923
                     /locus_tag="VYO12_000731"
     CDS             26201..26923
                     /locus_tag="VYO12_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1587377.1"
                     /translation="MIKHNELVIDGVRTSSFPFKIIVHDSPSIALGESKTALLEHGGV
                     SGAIVQTNKHRELVKKSYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVTDLEEVQPGLYMTKATFTCHPTKYFKGSDTQRLTRSGTLTVQGSALAFPKITIVG
                     QSAVETSFTIAGQVIRLEKLSESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSLEIETVWGWA"
     gene            26924..>28364
                     /locus_tag="VYO12_000732"
     CDS             26924..>28364
                     /locus_tag="VYO12_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1587378.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKETMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEYNDHLEITAYHISDDVMQRSITPVS
                     VTSQSCGMTLSRMVQNTKTALGDFSFNSDIQDRRTFNTTETETLYSILLDGKHSIVGT
                     WEGELVRDNFAMTVKKSRGENRGVVITTHKNLKDYQRTKNSQNVVTRIHARSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKSVEELQKWAQAKFSNEGIDKISDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDVFKKAIAYEFDALKEEYISLILDDKAGAGG
                     SRTSGGLSSAAYAILGVTESAQEVALEKALQNADLDFDHKAGLLRQEISDGIELAKAK
                     AEEVKQELSDTINQRFNSFDNGPLKEAKRKAEEALRNAGASSSLAQESKRIGLDSVAR
                     LEAFKSQTTSAQTALSGDLDALKRTIVNDI"
BASE COUNT         9061 a         4730 c         6424 g         8149 t
ORIGIN      
        1 ggcggtcttt ttagaacttt aatcgtttga aatttaggta gcaaatttgt ttctattctg
       61 tcaacttttc ctatttttat cttgttgagg ctggtatttt aacaattcag gaattgatag
      121 tgaatgtgta aaattttttg ttagaataag tttataaaaa agaaaaggag tatttgatta
      181 tgttacaaaa aatttatgag cagatggcta atttctatga tagtattgaa gaagagtatg
      241 gtcctacatt tggtgataat tttgactggg aacatgttca ttttaaattt ttaatttatt
      301 atttagtgag atatggcatt ggttgtcgta aggattttat tgtttaccat tatcgtgttg
      361 cttatcgttt gtatcttgaa aaattggtaa tgaatcgggg ttttatttct tgttgaggta
      421 attttagtaa atttccgaac taatttactc ttttatggaa agatgatagt aaatagctag
      481 taatttttct aaatcatttt ttaatagttg gaaatagcaa atctttctat tgtttcttct
      541 tgataaaaag acgatttttt attataataa attgtaagat ataattgcag gtgagagtcc
      601 tgccatgtat gtgagaaagg aagagcctga gggctcagac aagattatga cttcagttgt
      661 tgttgtaggt acccaatggg gtgatgaagg taaagggaag attacagact tcctttcagc
      721 gaatgcagaa gtgattgcac gttaccaagg tggtgataat gctggtcaca cgattgtgat
      781 tgacggtaag aaatttaagt tgcacttgat tccatctggg attttcttcc ctgaaaaaat
      841 atctgtcatt gggaatggta tggttgtaaa tcctaaatct cttgtaaaag agttgagcta
      901 tcttcatgag gaaggtgtaa caactgataa cttgcgtatt tctgatcgtg cgcatgttat
      961 tttgccttat catatcgagt tggatcgctt gcaagaagaa gctaagggcg acaataagat
     1021 tggtacgaca attaagggaa ttggtccagc ttatatggac aaggctgctc gtgttggaat
     1081 tcgtattgca gatcttttag ataaagatat tttccgtgag cgtttagaac gtaaccttgc
     1141 tgaaaagaat cgtctttttg aaaaattgta tgacagtaaa gcgattgttt tcgatgatat
     1201 ttttgaagaa tattacgaat atggtcaaca aatcaagaaa tacgtgatag atacatctgt
     1261 tatcttgaat gatgcgcttg ataatggcaa acgtgtgctt tttgaaggtg cacaaggtgt
     1321 tatgctagat atcgaccaag gtacttatcc atttgttacg tcatcaaacc ctgtagctgg
     1381 tggtgtgaca attggttctg gtgtcggtcc aagcaagatt gacaaggttg taggtgtatg
     1441 taaagcttat acgagtcgtg taggagatgg tcctttccca actgagttgt ttgatgaagt
     1501 gggagaacgt atccgtgaag tgggtcatga gtatggtaca acaactggtc gtccacgtcg
     1561 tgtaggttgg tttgactcag ttgtgatgcg tcatagccgt cgtgtttctg gtattactaa
     1621 cctttctttg aactctattg atgttttgag cggtttggat actgtgaaaa tctgtgtggc
     1681 ctatgatctt gacggtcaac gtattgacta ctatccagct agtcttgagc aattgaaacg
     1741 ttgcaagcct atctatgaag agttgccagg ttggtcagaa gatattaccg gagttcgcaa
     1801 tttggaagat cttcctgaga atgcgcgtaa ctatgttcgt cgtgtgagtg aattggttgg
     1861 cgttcgtatt tctactttct cagtaggtcc tggtcgtgaa caaacaaata ttttagaaag
     1921 tgtttggtcc taagagattt ttaagatttg tttaagatag gtcgggtata ctatagacgg
     1981 ttacaagaag acctcctaac ttgttgtaac aaatatccta aacttttctt tttcataata
     2041 atctccctta actccaccga atcaggtgga gttttttagc tctatttcag gcttttgggg
     2101 actattctaa aaataatttt tcgatatttt tcggtatttt tcggattttg gtcggggaat
     2161 tggcggggac ttttttagcg aatatgacta agaaataggt ctgttgtcgc ttcagcaagt
     2221 tcgtcctcta cttgattgta acgatccgtc atataaacct ttgtatgccc cagcgcctgg
     2281 cttaattgtt caagcggaac ccctgcaata atgctttgag tcgtgaagaa gtggcgcatc
     2341 atgtgaggtg ttacatgcaa tcctgttgct tcattcacta gattgaagtt tctatttagc
     2401 tgatttggat tgatgagacc acctttctcg ttgatagtta tataatcctt gtgctgttcc
     2461 ttgataattc ctaactttcg cttaatctta gaagcttcag ctatcagata atagatcagg
     2521 tctgttccga tatcatcaag gcagacatat cgctctgaat ccttcgtttt aagccctcct
     2581 ttccctttta aggtctggtt gcttcgactg tctctaagat gcagtatagc ccgtccgctg
     2641 tcgttctgag tgatgtccat tggacgcaat ccaaagactt ctcctcttct caatccaaaa
     2701 attgtcagat aggtcagagc gtagaattgt tttggcataa tctcttctgc ctttgctatc
     2761 caagtcttga actctttgag agtcactttc ttgtttgcag cagggatatc actctggccg
     2821 atgaaaacac ctttcaagcg atttgagagc agatttccat ttttgacggc atcattcagc
     2881 aatgccatga agctggaatt gagggtttga acagtgtatc tggtatggtt ctgcaacttt
     2941 tcagcgataa agagttcata ctcatttcta tccaaatttt taagcagggt agaaccaaac
     3001 tttgccttga tatggttctt gtagagatta tcattgaggt agtaggaagt gtcattccag
     3061 cgccctgttg acaatctctt ttcagaataa atatcccaat attgatcaag tgtcagattc
     3121 gtattgatac ctaattcttg ttcttggatt tgttgctcaa gctctactaa ggctgcacga
     3181 gcttgtggaa gggttgtgag accactttta cttttttctc tttttttacc tcggaagaaa
     3241 aaagaacgtc tgacatagta tcgcttgcct ttggcagtct catagtaaaa gatatttggg
     3301 tattttgttt tattatattt cattgtattc tccttgttta tcggcttctg gacaaggtct
     3361 aaacattgag aatattgaca tcaccccttt catggtgtaa aatagggtat agaaaatagg
     3421 cctttttaat ggctgatttc ttataaggct aagcttcaca atcaaacttt ggcgagggcg
     3481 attgtggggc ttttttgttt ttaattcaac aaaaaacggt aactaaattt atagttaccg
     3541 tttctgcgtg gcagcttgtg ccaaccaggt tatttgcact aggatttccc ctaggttagt
     3601 aactatatat tatcaaatat atttcatttt gtcaaaaatg gaaagttgag tcttcacact
     3661 cagaatttag agatagagag tgtggggatt tttttatttc ttgactttat cttttaaagc
     3721 cttctctatg gctttcttta attccaaaat agatgctttt tcatctttgg taaaggttac
     3781 tgtgttttca tcttttacgg catcaaacac accacctttt gtatcagaag aaccagggta
     3841 aacaagttga agatagccaa cagttgagcc aggttctttc aattggtatg ctgtaatctc
     3901 agagaggaga attgactttt cgccatcaag accatgaaga agaacattag atacatttga
     3961 ctttcttgca atcctgataa aataatcatc gattcttaca acagtttttg atttcttaaa
     4021 ctcaaaaact cgctcattcg gttcctctgt gaagagttca acctctaaac tttcatcttg
     4081 ctttttacca aacaatgcca taagtagttc ctttcttttt ctgcttcagc agtttataaa
     4141 catatttaac caattaaagt ctgatattcc tcttttacca tgacttcatt tgtcacggtt
     4201 ttaagattgt aataagacat gaatttgagg taatcaaact ctttggggtc gtctaagctt
     4261 tctagtgcgt cttttacgag atgatggatc atattcctat cagcttcgtt ttcacagcgt
     4321 aggcgagcgt tctggtattc tgatcgtgtg tgatccttgt gtcctagttc atgaagtagg
     4381 accttaactc tctctttttt gctgagttta ttagacaaga aagctgtatt ggtttctttt
     4441 tcgtaaaatc caagttcatc aggtattagc tcgccgtcaa aatcgacaat gcgaacctga
     4501 aaatgactta taatttcttt ttcggtcact aagcagtacc tctaatcacc agcttctttg
     4561 agataacctt caatgataga ctggatgatt ttcttctttt catctgttaa ttctcggcca
     4621 ccaaacatca tgacattaga tgccatttct tcaacattga gaactttgcc ctgccatatg
     4681 tactcttttg tatcatcggc aagattagga ttttcagtac ggcctagtaa gtagtctgta
     4741 ctaacaccaa aataatctgc tatttcttgt aatctatcag attttgggtt accttttttc
     4801 agactataaa gataatttgt gctgtatcct aatttttctt ctaaaatgtt taaagaaatt
     4861 ttctgtttat cagccaattc tttgattcta tcgaatgcta agaacattgt tttatcaacc
     4921 tttctaagca ctacgaaaaa aatattttaa aattaattat aaaaactgtt gacaaattat
     4981 aaaactagtt ttaaaatagt attcgtaagc taaagagtta gcgaactaga caactaaaaa
     5041 ataaagcctt acaaaactga ttggcgtccg ttttctatag gtaaaactta ctttagtagg
     5101 tcttttctct atgtctttat tctaaaacta gttttagaat ttgtcaagta gttcgctaac
     5161 tttttagata atttttttaa aaggaggtca gggatgagcc aacaacataa aaaatggatt
     5221 cggttagtta aggataaact gaattcagaa ggaatgacac aaacacacct tgctcgtgct
     5281 tgtggagtga agaaatctac catttcagaa ttattgaaat acggtaaagg tagcgacaaa
     5341 ttaaagaacc gagtttgcga cgttttaaga attgacgaaa cttgggttga gttaggagag
     5401 tagtatatga acgaaatttt taattttcac ggacaggaag tccgtacttt gacaattgat
     5461 aacgagcctt ggttcgtcgg gaaagatgtc gcagacatcc taggatatag taaggctaga
     5521 aatgcgattg ctcttcatgt tgatgaagag gacgccctaa aacagggcat ccctactagt
     5581 ggtggaacac aggatatgtt gatcatcaat gaatctggtc tctactctct cattttatcc
     5641 agcaaattgc ctcaagcaaa agagttcaag cgttgggtga catcagaggt cttgccagct
     5701 attagaaaac aaggcggatt tatccgtgag gatttggacg aggatgcttt catcgctctc
     5761 ttcactggcc agaagaaatt gcgcgagcaa caggcgacca tgctggaaga tattgactac
     5821 ctcaagagtg agcaaccgat tcatccgagc tatgctcagt cgctactgaa gaagcgcaag
     5881 gctcgagtag tcgcctgtct tggtggaatt gatagtccag cttatgctga taaaatattc
     5941 gctcagtcgg tatttagaca agctgagatt gactttaaag accacttcaa tatcagtcgt
     6001 tatgacttgc taccgaagaa gtttgcagaa gctgctcttg cttactggat gacgtgggag
     6061 ccaagcacca ataccaagat gaaaatcata aaattgaact catttgacga agggtaggag
     6121 gggaagaaga tggacaatgt tctactttca ctgtctgaat ggattaaatc tattatcaag
     6181 gacacaatca ctagattggt tgaaatagaa aaagatagtg atcactatcc agagctgatg
     6241 gatgtgaaca ctacctgtga ttttctagga attaagtatg ccaccttttc agataattat
     6301 cgttacttaa agggatttcc aaaggaatta cctggtaaga aatggtcaaa aagagccatc
     6361 aaagaatggc tctcgaatca aatataataa ctttactaaa aggcttctgg acaaggtctt
     6421 agcaaaatta tttgactata ttatagcaca aaaagaggat aaggagataa aaatgtttga
     6481 accaccgatt ttagaccagc tgatgggggt tggagccttg ctgcttggat ttgcaggggc
     6541 ttgccgtcat atcaaattgc aggaaaaacg caaggaagaa gaaagacgag aagagcaaga
     6601 atttgcgtct atgattatcc aagggtataa ccatgcatac gaacgtggta gagaggcaga
     6661 acgtcaagaa atccgcaaga attgccgtag agagttcaag ggtttcacat acgacaacga
     6721 accacctgta gacttgcgtc ctgagcctct agctttgcca gaacctaaac aatctgcaat
     6781 tagatttttg taatgaggag gttaggaaat gcaagaattg attgaatcgc tggataacct
     6841 gattatgatt gttaaagaac tggaaggaag ggaatcaact tcaagacatt ttattacgat
     6901 atgggaaaac gattataaaa atctattact agtcaaagaa tacctaaccg atgaaaaact
     6961 agcaaaggac tatcgttata tgacccttaa aaataagctg ctaaagattg aaaaaatgga
     7021 gctggaaggc aggtacatcc tatatgaaga tatgcggatg aagtatcgtg ccaatcgcag
     7081 gaagtggggt gctcggtatg tctgaaatca agtggataaa aatcaaaacg gatatttttg
     7141 acgatgaaaa gatgtgtcta attgatgcct cgcctgatcg tgatgcaatt attgtaatct
     7201 ggatcaaact tataacacta gcaggcaaat tgaatacaaa aggcgtacta accatttcta
     7261 aaaacattgt atacaccgat gaaatgcttg cacaaacgtt ccaccgtccg ttgaatacag
     7321 ttcgtatggc tcttgaagtt tttgaaaagt ttggaatggt tgaaaaaatc gatggagtga
     7381 taatgttacc caattgggag aaacatcaga acattgacgg catggaaaaa ataaaagagc
     7441 aaaatcgaaa tagagcagca cgtcaccgac aaaaacagaa attacttgca cagaacaacg
     7501 aaagtaacgt tactgataac gttatggata acgttacagt aacgcatggt aacgcactag
     7561 ataaagaatt agataaagat atagagataa ataataataa ggtgatgatt agttccagcc
     7621 tttctgaaaa tttgaaaaat agcggaatcc atctaactga taagtcacat caacagttac
     7681 ttgattatgt gggacttgat ggaatgagtt tcgatatgtt gaaccgtgca gtcgagaaaa
     7741 cttccggatc acacaaacct agtttcaatt acctaatagc cattcttgaa agttggaaaa
     7801 agaaaggctt cacatcgatt gagcaggtgg atgaggacga ccgtaaatat aaagagggta
     7861 agaagtcaag acaacaaaac gataaaacat cggaacagga ggtaagggac gaatggggat
     7921 tttagaacta attgagcagt tcgagattga ctattatccg ttaagctacg agaagaaaac
     7981 tcttttagca gaccaaccaa ttcatcaagt ggttgcctgc ttgtctgaaa tggctagctg
     8041 gcatgaatgc ggaggtcggc tagtatggta gacaatgtgt ttgaggaaat cgccttatct
     8101 tatcgtagga atacagaaca acaagaagag ttctgcgaaa agcataacat ccctttgata
     8161 aagatattga ggaccgagag tgttgtatgt cgcatgtgtg aatctgagcg gattcatgaa
     8221 gaaaatcagg aaagagtgaa tgaactggct aatgctgaga atgagcgaga gaggaaatac
     8281 tatcttgaaa agttctctct ttatgatgaa gttttgaaaa atgctacttt ggacaatttt
     8341 gaaacaccaa ccgaaaaaga agcggaaaag ctagcttttg caaagaggat ttgtcgtgag
     8401 tggtctgagg gtgctaggaa caacatcgtg ttacaaggag aagctggaac aggtaagagc
     8461 catttggctt ttgctatggt taaggctcta tctgagtaca cgaaagagat tgctatcttc
     8521 atcaacgtga cggacttgtt gatgaagatt aaagctgatt ttagtcagga ggagtttctg
     8581 gtcaataaaa ttgccaatgc taagttcttg gttttggatg atttgggaat ggaaaaggat
     8641 agcgaatggt cgtttactat tctctacaat atcctgaata agcgttcaaa tacaatcatt
     8701 accaccaatt tgacttctgc tgatattcag aaaagatatg gcagaccctt tatgtccaga
     8761 ctgatgaagg gtgtggataa agaccatttg atggttttca acgacttgac aaacaagcgg
     8821 aagcaatatt tttagaatgg aggtggctga tgtttatttt aagacatggg acaagagagg
     8881 ataagccgtt tctgaagtcc gtagttattg gtgtgactgg cttggacatt tcatgttcag
     8941 aggagaagaa agccatgcgg tttgtttctc gtggggcagc cgtacaggtt ggtaaggctt
     9001 tgaggggttc ctttgggaat ttttatcccg ttgaggtgga gtgatgttag agctttactt
     9061 cgtctacaac gggcactgca agttttacct tggaacgttt gacaatgtcg atgatctcat
     9121 tgaacagatg gaagatcatc agtgggcttt ctcggctatc actcatccaa gatttcagaa
     9181 gcacattggt cagcggacga cacggtttga ctacggttcg aaggattgtt actatttagc
     9241 gactttttca ggaggagaat aatggctaaa aatattttaa tggatctagc atttgagaat
     9301 cttcacaaat gtatgggaat ttctgattgg aaagaatctg atgaagtaat tcttgttagt
     9361 tcagctaata aagaacaaat tgagtcagat gaaagttacc attcagccgg aaaatgtaat
     9421 tatcttggca aacgaatttg tatcttctgt gaacaagtga agaaaaataa ttacatcact
     9481 ctacataaat ctatgttaga aaaaattatt aagacaatgg aatcatttac agatgcagaa
     9541 caaatgaagg gagaagaaaa tgattgaact tattaaagaa tttggcatgg cgattctgtg
     9601 gctatttctc ggttacttaa tcggggaacg tgcagcaaga aagatgatca ataacgttac
     9661 attggtaggg cgcttgacga aagaccctga attaaaatat acgccgtcga atgtggcggt
     9721 tgcgacgttt acacttttag tgggaggtaa gaaacatggt tggagtaacc tataaggaaa
     9781 ttcatctctt tgttgaattt ttgaaagagc agtacgggca aggtcgtcca gactatattg
     9841 aagccctgaa cgacttagac ggtctggtgg aagtctccta cagagaagct attgaaagat
     9901 ttttagaaga tgaagtacga taaacaggct gagattgacg gactgaaacg cacgatcgag
     9961 caaaacgaag agaagataat cgagtattcg aagccgtgcg atgcatgcaa gagacggatt
    10021 agagcgctgg agcgcgattt gctgaggaaa aagaataaag aattgagacg gaaagtggag
    10081 gagttggaag gtgatggaag agttaaagca aaaagttaat gaagtataca actggacggt
    10141 agaagacggg aagccgcaac ctcccaagca agatttacca caagtggtga aagaccgggc
    10201 ggactatttt tgggaaatgg cagaagatgg tatgacgttt atgggagcga tggaatgcat
    10261 cttcgctgat gaaaagccta cagactatga tttgggagct actaaggatt ggttgccaaa
    10321 atctaaggag tttgatgatt ggattggcta ttcgccaagc atggctcagg tagttattgc
    10381 agtttatttg atttatggag gaaactaaga tgaataagca ggaattgatt aaaaaattag
    10441 aggaacgaag aacaataact gggaattttc aaggttatgt agtttggtgg aaggatgtaa
    10501 aagaaatctt tgaacaacta ggcgaaccgc aaccagtcaa agtaccgcag tgtgtggcgg
    10561 aatatataga atttaaaaag aaaaacaatt ttcatgttta cggtgcaatg agagtaattg
    10621 aagatcatta tgataagaaa gttcctgatt ggttttacga aaataacatc gaaaaattct
    10681 gtcttgcttg gcttaacggc tacgaggtcg aaaaagagaa gcggtatttg gtgaaggtga
    10741 aaggtgtaca ttttactaat taccttattt ctggaaaccg aaaagatata tggttttttc
    10801 attcagctta tgaaccagaa catcaaataa tagcccacac ccgcaaagaa cttgaagaag
    10861 ccgggttcgg ctgggtgttt gattgcccag ggattgagat tgaggaggtg gagcgatgac
    10921 acaaacactt gaagaaggaa tgaagaatca aagtaaatgc ataaaagtcc caagggaaat
    10981 cagaccgttt gatatagggc atcgaatagt gaacaaagac ggtcaagcgc tcgctttaag
    11041 aaatggggca agtatattcg atttgccttt tctggctgaa aaagctatag aaaaagagtt
    11101 tgggaagaat gatccagact ttgacatcgg aaagcattct gttgaagagg tcgctattgt
    11161 caatttaagt aaatttcata gttactttga gggggtggag tgatgtcatg tagtgaaaat
    11221 ttaaaaaaag aaaaagaatt gactgctgct atttcaaatt tcaagataga agtcttacaa
    11281 aatgatgata aattgagcag tctatcatta agcaacatca aaaggcaagc aagggatcta
    11341 tatgaatgct tagtatggtt gcagtataat gcggaggaat caggtagatg agttatgatt
    11401 tggaaatatt agtaaaaata gagagtggag attatatttg tatcgctgaa cctaaatata
    11461 gttctccgat atacaatctt ggaagaatgt ttagagttgc tatgaactgg gattttgatc
    11521 aagacactac gtacaacatc gctgatgttt tagataacat tcaacgcggt atctctgaac
    11581 tagaacggta ccctgaaaag tatgtgcagt atgaacctga aaatagatgg ggaacagtta
    11641 gcgttgcatt ggaggtttta aagtcactga aagagtgtat tttagaacaa gatattgata
    11701 cgaaatattt atatatgagg tggtaatatg agacgattca tagctatctg gattcttgtc
    11761 tctgccggat tgaacatctg gcatatggac aggattcgag atttggaaga gaaaaagccg
    11821 atggttgtct ataaagctga taacgctggc gctgagatat tcgggcgtgt cgttgaaaaa
    11881 ggacggcatg ggaagttgta tacagtaact atcagagact atgggatttt cgtagttacg
    11941 aaagagcagt ttgagaaaat cagagtaggg gatgaggtgt tactctaatg gatgatattt
    12001 tacaagcttt agcaaaaatg ctaaatatga ctgttgatga agtaagttct ttgcttacaa
    12061 catttaaagg gaatgcacca cagatttatg aaatgttcgt taaagaaaag atgttttatg
    12121 atctcttcag tctttttcaa atcatgtcaa ttgtaatatt tagtatttct gcagtagttt
    12181 tagcagtttt aactctcata tattttacat acgatggtgg ttttgtttat tcctatgata
    12241 tacgtacagg aaaaaccgag gaagaaatta aattagaacg cattgaacgg aaaagaaagg
    12301 acttaaaaat accactaaaa attagttgca tttcatcaag cgcaagtttg ataacagtag
    12361 ttattgcaat tgttttaaaa gcaactcttg cacctaatta tatattcatc gtgaatgaga
    12421 ttttaccaaa attaacgaag agataggagt tatcatgaac acaatagaga aagtcaaaca
    12481 atggtttatt gaccgtgatt tagaaaacgg tagggtcgtt ggattgatgg tgctttcgtc
    12541 aaagaggagg atttggcatg atactgaaat ttagagtgtg gcatcatgaa ttaggtagac
    12601 tgatgtcagt caaatgcatg ttttttcagg atagcgagat tgaagaattt gagttaaacg
    12661 atactttaat gaatgattac attacagctt atcctgatga aatcgaactc atgcagtcaa
    12721 cagggatttt ggataaaaat ggcaaggagg tattcgaggg ggatatcgtt cgatttttcg
    12781 atagtctata tactgttttt tacgatatca aggatagatg ggttgtagat tatatgtcta
    12841 atttttcaag cgaagaaagt attgaaatca tcggcaacat ctacgaaaat ctagtttaca
    12901 ttttttaaaa acttggaggt ggaaattgaa aaaattgagc gacgaagacc tcaaaacatt
    12961 agacagagaa ctttttaaat ttcaaaacat tcaacgtaca atagatttga gaaggctaga
    13021 attagaaact cgaaacccag atgctcagag tagtcccagc gtaggaataa gcaaacctac
    13081 cgaaactatc gcagtcagaa tcgcagatga tccaacctta aaatttctcg aagggttcaa
    13141 agctattatt aacaaactct tgatcaatct agttgatgaa gataaggaaa tctttaatct
    13201 gcgctggaga tatcctcaac tgagatggga agaaatagca gaacagaaat tcatgagcaa
    13261 agctacaatc tatcgacgta gaaggattat cttagaacag tacgctatac tgaaaggtga
    13321 gttgtaaata agattgagac aaaagacatc ttgaagtctc acaaaaaaag gtctattatg
    13381 atagcatgaa cttctgaaac aaaaacacac atcacacttg aggagtcatc cttaattcta
    13441 gtccaaaagt tgtccaacag aagtatcgtc aagagtcagc aaatgctggc tttttgtttt
    13501 tggaaaggag gtagaatatg gaatttgtat caccgataaa agataatgac gacattcagg
    13561 caatgaaaga ttatcttaga gagtggaatg agatgtatta tatgctattc attacaggcc
    13621 tgaatactgg tttgcgagtc ggagatatac ttaccttgaa agttaaagat gttcaaggct
    13681 ggcacatcaa actgagagaa cggaagactg gcaagcagat aacaagacgg atgacaaaag
    13741 aactcaagaa agaaatgagg agatatgtcg agggcaaacc atttcatcat ttcttattca
    13801 agagtaggca aggtcagaat aaagcaatca ctcgtgagcg agcctatcaa atcatacatg
    13861 aagcagctga agaacttggc attgataatg ttggcacaca tacaatgcgc aagacattcg
    13921 gctataaata ttacaacaag acaaaggacg tagggacatt acagaaaatg ttcaatcact
    13981 catcacctgc aattaccctg agatacatag ggatagaaca agcagagctc gatgatgctt
    14041 tacggaactt tgtcatttaa tttttttaga tattactttc acataatgag ttaagcataa
    14101 actgaaaaaa tgaaactctt taaaacccat gcttagtaag ggtttgagat ttagagtgag
    14161 tttaacaaaa tataagatat gtgaaagtga gggataaaat tggtatagtt acaagaggtg
    14221 aaaaatggta aaagaatacc gtgatgattt tcttggagaa aaggccttcg agaaattaaa
    14281 taaagatatt gatgccaatc ctgaagttgg ctttgaaatt gttggatata ctcaaacagc
    14341 atttgtaaat ggaatgcata taccgttaac agccatacta gtaaaatgga ataatttttt
    14401 taaagaatca gaatgagaca aaagacatct tgaagtctca caaaaaaagg tttattatgg
    14461 tagcatagat ttcttgtatg aggaggggat aggtcaaagg cctgtccctt ttagcattga
    14521 gaaaggaggt ttgagatgta taacaaacct atcagaccat ccttgaaatc taagaagtgg
    14581 gagaagttcc gtgataggat aatgcgtaag catgattatc tttgccaaga aagtttgcgt
    14641 tacggaattt ctgttcaagc agaaatggta catcatatct ttcctgtgtc tgaatatcct
    14701 gagcttgaat ttgttgaatg gaattgcttg ccattgacga ataagaaaca taatacgttt
    14761 catgatagag tgaacgatag agtaatcaac caaggattgt attggcagaa aaaaagaaaa
    14821 aaagaatttt taaatttttt caaaaatgaa aaatgaaaat ttttaatccc cccctctttt
    14881 tgaaaaatca ttttggccag tagggtaccg gtgaagggaa ctttttccaa gtcgggggcc
    14941 ttcaaacaaa aagggggtaa aaactaagcg attttgacga aaggaggtag tttttggcta
    15001 aaccaattac agcaaagtcg attaagtcaa aagtggtcaa gcagatgaaa gacttgggaa
    15061 cttatcgtaa agagttcgaa atgatcattg acatttttgc aggtatgtta taccagtatc
    15121 agaaacttgc tcaagattat gctgatatgg gttatccagt aacagacacc tacgtcaata
    15181 aggctggtgc tgaaaatgag cgcaaagttc caatcttgac agcgatggaa attttgagga
    15241 aagatatact cagctactct aatcagttga tgatgaatcc taagtcgctc ggtgaggtag
    15301 tagaacaaga gggtgagtca gttcttactg aggtactgaa gttcaagaac gaaatcaaga
    15361 agaagcgagt gactggcaat gggtaatctt gataaagcta aaaaatacgc tcaacacgtc
    15421 ttaactcacc gagaagaaca ttgcgaagaa aacatccttg ctgctgaacg ttttttccgt
    15481 gatttagaaa atccaacctt tgagatggat gaggatatgg tggattttgt tattcacttt
    15541 atcgagaacg tgatagttca tcagcagggc gatgatatgt ttgcggtatc tatccgtaac
    15601 aagccattgc ttttgcaacc gtggcaacat ttcgttgtag ttaatctgtt tggtttttac
    15661 tacaagggta cgaatgagcg caggttcaaa gaagcgctta tcatgcttgc tcggaagaat
    15721 gggaagacct cgtttactgc tgcaatcgca cttgcttatc agatattaga tacagatagc
    15781 ggttccaaat gctatatcgt tgctaactca gtcaagcaag cgatggaagc ttttggtttt
    15841 ttaagattca acgttgaacg ctggaacgat aagaacattc gtatcaagga taataaccaa
    15901 gaacactcta tcaccgccaa ttttggtgat gaaggttctt tctttatcca ggctttagcg
    15961 aatgatgaga gccgtctgga ctctttgaac ggaaatgtta tcatcttgga cgaagctcac
    16021 acaatgagga acagtaagaa acatggtctt atgaaaaaaa caatgtcagc atacagaaac
    16081 agtatgcttt ttgttatctc aacggctggg gatattccta ccgggttcct tgctaaccgt
    16141 ctgaaatact gtcaaaaggt gctcaagcaa ttagtcactg atgattcatt tttcatcttc
    16201 atctgcaagg ctaatcaatc tgctgatggg gacgtggtga actatctgga cgagaatatc
    16261 ctcaagatgg ctaatccgtc atggggtgtc acggtttcgc tcaaggctct caaggaagaa
    16321 gcagagcagg ctatgaatga tcctcagaca agaaatgagt ttttcaataa gaccttgaat
    16381 atcttcacta actctatgaa cgcttatttt aatcctgatg agtttattgc gtcggatagt
    16441 tgctacgatt ggagtttaga agagctggca cgtttgccta ttcgttggta tggtggtgcg
    16501 gacttgtcaa gattgcacga cttaacagct gctgctctct atggtgtcta tcatgacggt
    16561 gagaaagacg ttgatatctg tatcacacat gctttcttcc ctcggattaa tgctcagaaa
    16621 aaagctaatg atgacgggat tccactcttt gggtggcagt ctgacggttg gttgacgatg
    16681 agcaacactc cgaccgtcct ctatgatgat atcgtcaaat ggttcatcaa gatgagggag
    16741 aaagggttca agattgctgc tgtcggaatg gataggaagt ttggccgtga gttcttgacg
    16801 aagatgaaac aagctcggtt caagatgatt gaccaacctc agctttttta tctgaaatca
    16861 gagggattca gacggattga gtttaaagtt aagaataaag aattttacta tcttcattct
    16921 gatgcttacg aatactgtgt gagcaatgtt agagcaattg aaaaggtgga cgatgctgtg
    16981 caatatgaga aattagatgg agacggtggg actgcaagga ttgacttgtt tgatgccagc
    17041 gtctttgctt gtatacaggc tcttgctaat cttggcaagg gtggtgatgt gatgagattc
    17101 tttgattagg tgaattatga atgaaatagt attatcagaa catgaaatta acttgctgat
    17161 caacaaaggg cgagttaaag taattttaaa cggggaagta gtaactattc gtcaaagaca
    17221 tatgaaaaat ttgatggctg aaacagtaaa atgggaaaaa caggtaattg atgtcagtca
    17281 gaatatcgta agaaataaac actttgattc actttttcaa aatacttttc gttagaaagg
    17341 aggtgaggaa acatgggtat ttttgaaaag ttttggaaac gaaacaagcc aagtaagtca
    17401 atcaacatgc tgagtcattc agatttaggg ttgtcaaacc tgatggactc gtatgtacct
    17461 ttggccagaa atccagatgt ggtgacagcg gttaataaga ttgctgattt ggtctctaat
    17521 atgaccatcc acttgatgga gaatacagat aaaggtgata tcagaatccg tgatgggctt
    17581 gctagaaaga ttgatatcaa tccgtgtaaa cacatgacaa ggaagtcatg gattttcaag
    17641 attgtgcgcg atttgctttt atatggcgat gggaactctg tcctacatgt ggaatatgaa
    17701 cctgttacgg attatatttc taatctaaga ccatttccga tgagagaggt ttcattccaa
    17761 acagataagg attcctatgt aatctcattt aggggtgaag aatattcccc tgatgaagta
    17821 gtccacttcg tcatcaatcc agatccagat attccataca ttggtactgg ttttagggtg
    17881 acgttgacag atgtggttca aagtttgaac atggctacca agactaaaaa aagctttatg
    17941 aacgggaaga atattcctag tcttatcgtt aaagtagact cgtctagtgc tgaactagat
    18001 tcggagcaag ggcgtgagcg tatcgctgag aagtatttga gtactagcag ggttggcgct
    18061 ccatggattg ttccagaggc attgctggac attcagcagg taaaaccgct tagtctaacg
    18121 gacatcgctt taaacgagtc tgtggaattg gataaaagaa cagttgcagg tctattagga
    18181 gtacctgctt ttattttggg tgtaggagag ttcaacaaga cagagtataa caactttgta
    18241 aatacgactg tcatgagtat tgctaccact attactcaaa cactaaccag ggacttactt
    18301 ttgtctagca atcgttactt caagctaaat cctcgttcac tcttctctta caacattacg
    18361 gagttgtctg ctgttgctca acaaatggca aacagtgctg caatgcgtcg taatgagtgg
    18421 agggattggc tagggatggc tcctgatcct gagatggaag agttgattgt tcttgaaaac
    18481 tttctccctc aggagaaact aggagatcaa aataaactga agggaggtga ggaagagaat
    18541 gcaaaagcga aatagctatc gtgccactca atttcaaacg agagaagaag aatctggtga
    18601 tttgattttg agtggctact ttatcaagtt tgacgaggag acggaattgt ggccaggcta
    18661 ctgtgaagtt atcaagcgtg ctggtgttga gaaagctgtc acagacgctg atatcagagc
    18721 tttatttaac catgatgata gccttgttct cggtcgaaca ggtaacggaa ctctgacact
    18781 gggtgttgat gatgttggtc tttttggaga tatcatcatt aacaaggatg atcctcaagc
    18841 tgttggggcc tatgcccgtg tcaagcgtgg agatgttata gggtgtagct ttggctttat
    18901 cccggtaaaa atcgaaacgg aagaacgtga agatggttca tatttggaca ctgtcttaga
    18961 gctagaaatc tttgaagtga gtccatgtac tttcccagcc tatccacaaa cggaaattgc
    19021 tgcacgacaa aaagacttcg aaagtcagaa gcgtgctaat cgtgaagcgc tagacaagcg
    19081 caagaaagaa attaaggaga aatttaatct atgaacaaat tattgatttt gggcgctcgt
    19141 atgcgcaaca aagcagacaa agttgtagag cttgaagaat caatcaaaga attgaacaag
    19201 cgttctgaac ttgaagctaa gaaattggaa caagctggaa ctgacgaaga agtttcagca
    19261 gttgaaaaga accttgaaga catccaaaaa gaatcggatg aaaaggaagc agaaaaagaa
    19321 caacttgaaa aagaaatcga agatttgaaa aatcaagttg aagaactaaa tcgtaaagca
    19381 ccgacttacc caagtcaaga aaaacgtgga ggacagaaat tggaacaacg tgacgcaatc
    19441 gctaaataca ttcgtactgg tcaaactcgt gacattgtag gcttgaaaac tactgattca
    19501 ggaagcgcag ctttaatccc tactgaagtt ttgaaacctc attttgttaa taaaacacgt
    19561 aatccacttt tggatcttgt ggaacgtgtg aaagttaata gtggatctgg taaatatcca
    19621 gttatcaaga aaacggatgg tgtaatggtt tcaatagatg aattgaaatc aaatccagaa
    19681 ctcggaaaac cagcaatcag cgagattgat tattcaatca agacttaccg tggatatgtc
    19741 cctgtgtcac aagaaatgat tgacgacgca gactatgaca tcatgtccat tgttgaagac
    19801 gaagtgttca atcaaggtga caacactgaa ttgtcattag ttacagctgt cctcaaaaca
    19861 gctacccaag cagatgcggc tggatttgat ggtattaaag atatctacaa caagaagctt
    19921 aaatcaattt ataaagcaag catcgttgta actaagtcaa tgtttgctgc gcttgacaaa
    19981 gtgaaggaca aagatgggcg ctacatgctt caaactgatg tggcttcacc tactggctat
    20041 tcatttggtg ggaaaacaat ctacaaagta gatgacacag tgtttggaaa cgaaggagat
    20101 atgaaattct tcatcggaga tgttactgag ttcgtcaaag agtttgaccg ttctcaagta
    20161 tccgttaaat gggtgaacaa tgacatttac ggacaattgc ttgggctttt tatccgtttg
    20221 gatattaaga aagcagatga agaagctgga ttcttcggaa catatactga tgttgtagct
    20281 taaggaggta gcgtatgagc tataaagtaa tccgtccttt caaggacttg gctgatcctg
    20341 aaaatcatga ctacgctgtt ggcgatatct ttcctcgtga gggatatgag ccaacagata
    20401 gctttaccaa tggccttttg actggcgcta acactgctgg ctctatcttc cttgaggttt
    20461 tgggagatga tgaacctaag aaaccagcgc ctgaaacaaa agaagtgaag gaagagcccg
    20521 cagttgagca ggaagaaaca gttgaggaaa ctgctgaaga gtctgctgag gaagttgagg
    20581 aatagacatg gaccaaggtc agcttttaga gttgctgaag cttaagttgg gtatttcaac
    20641 cgacttgaga gacaagccgt taaaaaaaat catttcaagt gtcatcactg aattgaccga
    20701 taatctcggt atcgagcttg ttggtgagcg tgctgaccat gaaatgttta tcgttgacta
    20761 tgccgcttat cgttatgagg gtggggtgga tatgccacgt caccttcagt ggcgactgca
    20821 taatttacag atagcatcaa agaaagaggt caagaatgtg gaatcatgaa atcacactga
    20881 tctccaagaa agtaacaggt aaggataagt tactacaacc aatctctgaa gatgttgaag
    20941 ttactctctt atgtcgcaaa aagagggtca ctcgctctga attttatcag gcgaatcagg
    21001 tagggcttaa accgagcttg gtcgttgaga ttcgaaattt tgagtatgag aatcaagagc
    21061 ttgcgaagtt cgaaggcaag caatatcata tcttgaaaac ctatcctatt gattctgaaa
    21121 ttttagagtt gactttgtca gaggtgttga aatgagtaat gaccttgctg atttgatagc
    21181 gaaagagctt gcagcttact ctgatgaggt tactgaagaa gtggataaga ttgcagagca
    21241 agtgactgat gagactgtgg atgagttgaa agagacaagt ccgaaacggt acggaaagta
    21301 tcgtagaagt tggaaaaaga agaagttggc caatggctct tttgttgtgt tcaacgcagt
    21361 tgcaagtctt actcacatac ttgaaaacgg gcacctttca agaaatggtg gtcgtgtcgc
    21421 tggtatcgtc cacatcaagc cagctgaaga aaaagcaatt cagaactttg agaagcgaat
    21481 caaggagatt gggaaatgaa gctatcagac tttgctgcta ttttggaaca ggcaaacttg
    21541 cctgtcactt atcgagcgtt taaaattgga aacgctcctg acctacctta cctggtctat
    21601 tatgaatcaa gtccagtcat caattcagct gacaacacgg ttaatcatca gattaagagc
    21661 gtgacagttg agctggcttt tgagagtaag gatgaagatt tggaagaacg tctggaagag
    21721 ctgtgggcaa accacaagct cttttttgaa gttcaagaag aaacatttat cgagactgaa
    21781 agactctatg tcaagtctta cacagtctat ctatactaag gaggaatgac atgactcaag
    21841 aaaataaagt aacctttggc ctagaaaatg tacatatcgc acctatcaag acacttgcag
    21901 cagatggagt tatcacttac ggcgatgttt ttcgttttcc tggagcaata gagctgatac
    21961 ttgatactaa aggggaaaca acccctatca aagcagacaa caaggattac catttcatga
    22021 attcaaacga aggatatgag ggtaaactta aaatcccaca catcattgat gaatttgcga
    22081 caaaaattct tggtgaaatc aaggaccctc aaactggtgt tatgactgaa aaagcagatg
    22141 cgagcttgac agagttcgca atgatgttcc agtttgaagg cgacaaaaac aagactcgct
    22201 atgtgatgta ctactgtttt gccagtcgcc catctcttgg ctcaaaaact aagaacggga
    22261 catcaaccaa cgaacgtgaa cttagtttca aagctagccc gcgtccattg gatacagttg
    22321 tcaagcgttc tatcacatca gctgatgaca aggatgcgta tgacaactgg ttcaagaaag
    22381 tgtatgaacc tactgcagtt gcagcttaag gagaagatct atgcgtaaaa tcgttttggt
    22441 tggtgatcag gagtatgagt tggggaccaa cggctatact cctattgctt acaagcaaca
    22501 atttggaaaa gattattttc aagatttgtt ctcgatgttg aaaaatcaat cattcatgaa
    22561 tgaattgaac aagttggaaa ccgacaagga attgacagcg actaatattg atatttcgat
    22621 gttgtcagat tttgacatga cctttttcaa ccgtcttttt tggacctttg ctaaatctgc
    22681 aaatcctcac atcaagcctt atgaacaatt cttcatggaa atggaaatct tcccgattca
    22741 ggaagttgga cctgtgctga tggaaatgct aaatgcgagc gtgacgacaa aaaagcacca
    22801 gatgagtcag aatcagctag cgaagaaatc ttcacagtag aatcctatct atcttgctgt
    22861 aaagaaactg gtctttctat tgatgatcta aagcacattt caatcggaat ggctctggat
    22921 tatcagacgg attatgtgaa tctacggagc gaggataagg gtggcgaacg gaaagccacg
    22981 caagctgatt ttgacagttt ttaaagaaaa atgagtgctg agagagcgat tctgagacca
    23041 agttccttgg tctggctgca ttatcagtgg tagaagttct ctcagcgctt ttctattttt
    23101 ttgagaaagg aggaaatatg gcaggaaata tcaaaggtat caaaattgaa atcgatggcg
    23161 acacgcaacc cttgcagaag gcgttaaaag ctattaataa agagtctgtt aatactacaa
    23221 atgaactaaa acaaattgat aaggctttaa agtttgacac tgggaacgtt attttactaa
    23281 cccaaaaaca agaagtctta cagaaacaaa taggtataac cagagacaaa ctagaaactt
    23341 taagacaagc ccaatctaaa gtagatgagg aatttaaaaa ggggaatatt ggttctgaac
    23401 agtatcgcgc tttccagcgt gaagtagaag tgactcaaaa tgtcctaaaa ggatatgagg
    23461 gaaagcttgc tagtgtcact caagctcttg aaggaaatgg tgatgcagcc aagaacaatc
    23521 aagctcaact aaaagaattg cagaatgaac aaaaattgct tgctagtgaa tctgaaaaag
    23581 tagttagttc atttaaactg caagaaagcc agatgggtgc caatgctagc gaagcagaca
    23641 agttagcatt agccgaaaaa aagattggcg cacagtctga aatcgtcact cgtcaaatcg
    23701 aaaaccttga gaagcagtta agcctaacta aagaacagta tggcgaaaac tcagccgaag
    23761 ctaacaagat ggaagcagag ctaaatcaag ctaagaccgc ttacgctaat cttaatcagg
    23821 aattaggaaa acttggtagt acagctaaga gcaaccaaac gcaactgaaa gaattgcaga
    23881 atgaacagag tcaacttgct tcagagatga gtaaggtgac aagttcattc aaactgcaag
    23941 aaagcgcttt gggttcaaat gctagtgaag ccgagagaaa tgctcttgcc cagaaaaaga
    24001 ttggtgccca gtctgagatt gtaagtaaac agatttcaaa tctagaacag caattggaaa
    24061 tcactaaaaa agaatttggt gagaactcca cacaagccaa caagatggaa tctgagctaa
    24121 atcaggctaa gactgctttt aatcatctca atgatgagat gaaggtaaca aagtctgctg
    24181 ctgatagcac tcaagaaagt ttaagtgaaa tctcaagaaa tttaagagca gaactacttc
    24241 aacagtttag tgagaagttg agtgctattt cagaaaagct tgtggaagta ggaaaagaag
    24301 cgttagaagc agctgctcaa atgcaagcta gtaatgctca atttactacc gttttcggag
    24361 atatggaaac ccaagcaaga gaagcgttga atgctattgg tcaggaaatg gatattgtcc
    24421 cagagcgatt gcaaggatca ttcactcaga tggcttcatt tgcaaaaact tcaggattgg
    24481 atacagcaga agctttggat cttacttctc gtgcaactag ggcagcagca gacggtgcag
    24541 ccttctatga caaatctatt gagagcgtga cagagagctt acaatctttt ttgaagggaa
    24601 actttgctaa cgatgccgct cttggaatct ctgcgacaga aacgaccagg aatgcagctg
    24661 caaataaatt gtacggaaag tcattcaagg acttgagcga agcgcagaag caattgacct
    24721 tgcttcagat ggtcgaagac ggaaataaac tctcaggagc tcttggacag gctgcaagag
    24781 aatcagacgg cctagaaaac gtgatgggga atctgaaaca agctgggacc aatgcattat
    24841 ctgctattgg tcaacctctt ctagaaatga tgattcctgt ttttcaaacc ttggcaacga
    24901 ttgtgaaagg tgtagctgag ctgttcagtt ccttacctgc tccagtaaaa gatttcattg
    24961 tcatcttagg tgtggttttg acaattgtag gagccttagc ccccatattc ttaaccctgc
    25021 aagctgtgtt tatgtcttca tttggcgcaa tgattgcagc agcattacca atcattggaa
    25081 ttatttcagg agttgtagtg gccatagcag cgattgttgc tattgtgaaa tatctctggg
    25141 aaactaacga aggttttcga gatgcggtca cgaccgtttg gaatgcgatt cttgaagtta
    25201 tcaatgcagt cgtatcagag atttctaatt ttgtcatgag tatctttgga acggttgtta
    25261 cttggtggac ggagaaccag gaacttatca ggacaagtgc tgagactgtc tggaatgcca
    25321 tttatacggt catcagtaca atactggata tacttggccc cttgctccaa gctggctggg
    25381 ataacattca actgatcatt acaacaactt gggaaatcat caagatcgtt gttgagactg
    25441 caatcaatgt tgtccttggt gttatccaag cagttatgca gatcattact ggtgattggt
    25501 caggagcttg ggaaactatc aagggagtgt tttctactgt atggcaagct attcaaagca
    25561 ttgttcagac tattttctca gctatccaga gttatatttc aaatattctc aacggcattt
    25621 caggaactgt atcaaatatc tggaacagca tcaaggacac tgtctcaaat gtgttaaatg
    25681 ctatatctag tactgtatca agtgtttggg aaggtatcaa gagtaccatt tcaagcgcta
    25741 tcaatggtgc aagggatgct gtttcttcag ctattgaagc catcaaagga ttgtttaact
    25801 tcaacatcag ctggccacat atcccactac ctcactttta cgtgagtggt tcggccaatc
    25861 cattagattg gttgagtcaa ggtgttccaa gtattggaat cgaatggtat gccaaaggcg
    25921 gtatcatgac gaaaccaact atctttggaa tgaatggtaa taacataatg gttggtggtg
    25981 aagctgggaa tgaagcagta ttgccactta acgacaaaac gcttggagcc atcggtcgag
    26041 gtatcgctca aactatgggt ggaacttcac caaccatcaa cattactatt actggtaaca
    26101 ccgtcagaga agaagctgac atcagtcgga ttgctgatga ggtggctcag cgtattgctg
    26161 acgaattaca acgtaggaga caattgagag gagggtttgc atgataaagc ataatgagct
    26221 tgtgattgac ggtgtgagga catcgtcttt tccatttaag atcattgtcc atgactctcc
    26281 ttcaatcgct ctaggagaga gcaagacagc tctcttggag catggtggtg tcagtggggc
    26341 aattgttcag acgaacaagc acagggaact ggtcaagaaa tcttatacga tttacttagt
    26401 caaaccgact gaggagcaga tgaaccaatt tatgagtctg tttatccgtg agaagttctg
    26461 gttagagagc gagcgagtca aaacaactcg tctctggtgc tataaggtca atgtgactga
    26521 ccttgaagaa gtacaacctg gtctttacat gaccaaagcg accttcactt gccatcctac
    26581 aaaatacttt aaaggctccg atacacagag attgacaaga agtggaacct tgaccgttca
    26641 aggttctgct cttgcttttc ctaaaatcac aatcgttggt cagagcgctg ttgagacttc
    26701 atttacaatc gctggtcagg tcattaggct tgaaaaactc tcagaatcgc ttgtgatggt
    26761 taataatcct gacaatccta gctttaaaac gacaacaggg aagccagtga aatggtcagg
    26821 ggattttatc acagttgatc cagcgaaagt gaagaatatt ggggttgttt tgggtccagg
    26881 tattcaatca cttgaaattg agacggtttg ggggtgggca taattgcttt atctacttaa
    26941 taaagatgtg agaaccgttc ggtggaacgg ggagccactt catgaagcga cttcggcgat
    27001 tgttaaagag accatgaatg gcgatttcac cttaactgtg aaatatccca tttccgactc
    27061 tggtatttat cagctcatcc aagaagatat gttgataaaa gcgccgactc ctgttcttgg
    27121 tgcgcagcta tttcgcatta agaaacctgt tgaatacaat gatcatctgg aaatcacagc
    27181 ctatcacatt tcagacgatg tgatgcaacg ttctatcaca ccagtaagtg tgactagtca
    27241 gagctgtggc atgactcttt ctcgcatggt tcaaaacacc aaaactgctt tgggagattt
    27301 ttctttcaat agcgatatcc aggaccgtag gaccttcaat acgactgaaa cagaaaccct
    27361 atactctata ttgctggacg gtaagcacag cattgttggt acatgggaag gcgagctggt
    27421 tcgtgataac tttgcgatga ctgtcaagaa gagtcgtggg gagaatcgtg gtgttgttat
    27481 tacaacacat aagaatctga aggactacca acgaaccaaa aacagtcaaa atgttgttac
    27541 aaggattcat gctcgatcca catttaagcc tgaaggtgct gaaaaggaaa caactatcag
    27601 agtgactgtt gatagtcctc ttattaattc ttatccttac ataaacgaaa aagagtatga
    27661 gaacaacaac gcaaaatctg ttgaagagtt gcagaagtgg gcacaggcta agttctcaaa
    27721 tgagggcatt gacaagatct ctgacgctat caagattgaa gcttatgaac ttgatgggca
    27781 agttgttcac atgggtgata cggtcaatct caagagctgg aaacataatg ttgatgtatt
    27841 taagaaagct attgcttatg agttcgatgc cttaaaagaa gaatatatct ctctgattct
    27901 cgatgataag gcaggagctg gtggttctag aacatctggt ggcttatcta gcgcagcgta
    27961 tgccattctt ggagtaacag aatctgcaca agaagttgcc cttgaaaagg ctcttcaaaa
    28021 tgctgactta gactttgatc ataaggctgg attgcttaga caggaaattt ctgacggcat
    28081 cgaacttgcc aaggccaagg cggaagaagt caagcaagaa ctgtctgaca ctatcaatca
    28141 gcgctttaat agctttgaca acgggccatt gaaagaagct aagcgcaagg ctgaggaagc
    28201 tttgcgaaat gctggcgcaa gtagttctct tgctcaggaa tccaagcgga ttgggctgga
    28261 ttctgttgct agacttgaag cgtttaagtc gcagactacg agcgcacaaa cggctctgtc
    28321 aggtgacttg gacgctctga aacggactat cgtgaatgat attc
//
LOCUS       DAVLXF010000018        28008 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLXF010000018 DAVLXF010000000
VERSION     DAVLXF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.018"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            71..568
                     /locus_tag="VYO12_000733"
     CDS             71..568
                     /locus_tag="VYO12_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1587379.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            568..2223
                     /gene="dnaX"
                     /locus_tag="VYO12_000734"
     CDS             568..2223
                     /gene="dnaX"
                     /locus_tag="VYO12_000734"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1587380.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLAGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2251..2445
                     /locus_tag="VYO12_000735"
     CDS             2251..2445
                     /locus_tag="VYO12_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1587381.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2595..3365
                     /gene="sufC"
                     /locus_tag="VYO12_000736"
     CDS             2595..3365
                     /gene="sufC"
                     /locus_tag="VYO12_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1587382.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3393..4655
                     /gene="sufD"
                     /locus_tag="VYO12_000737"
     CDS             3393..4655
                     /gene="sufD"
                     /locus_tag="VYO12_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1587383.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4666..5892
                     /locus_tag="VYO12_000738"
     CDS             4666..5892
                     /locus_tag="VYO12_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1587384.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPAIARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5879..6319
                     /locus_tag="VYO12_000739"
     CDS             5879..6319
                     /locus_tag="VYO12_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1587385.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6373..7785
                     /gene="sufB"
                     /locus_tag="VYO12_000740"
     CDS             6373..7785
                     /gene="sufB"
                     /locus_tag="VYO12_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1587386.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVICELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8016..9257)
                     /locus_tag="VYO12_000741"
     CDS             complement(8016..9257)
                     /locus_tag="VYO12_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1587387.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9360..10469
                     /locus_tag="VYO12_000742"
     CDS             9360..10469
                     /locus_tag="VYO12_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1587388.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11115..11462
                     /gene="ssrA"
                     /locus_tag="VYO12_000743"
     tmRNA           11115..11462
                     /gene="ssrA"
                     /locus_tag="VYO12_000743"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11645..11732)
                     /locus_tag="VYO12_000744"
     tRNA            complement(11645..11732)
                     /locus_tag="VYO12_000744"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11696..11698),aa:Ser,seq:gga)
     gene            11894..12634
                     /locus_tag="VYO12_000745"
     CDS             11894..12634
                     /locus_tag="VYO12_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1587389.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKYVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPAVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12631..13542
                     /gene="pfkB"
                     /locus_tag="VYO12_000746"
     CDS             12631..13542
                     /gene="pfkB"
                     /locus_tag="VYO12_000746"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1587390.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAELIKETYGKVEVEKR"
     gene            13539..15491
                     /locus_tag="VYO12_000747"
     CDS             13539..15491
                     /locus_tag="VYO12_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1587391.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YVAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15655..17880
                     /locus_tag="VYO12_000748"
     CDS             15655..17880
                     /locus_tag="VYO12_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1587392.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGSSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(17925..18353)
                     /locus_tag="VYO12_000749"
     CDS             complement(17925..18353)
                     /locus_tag="VYO12_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587393.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            18491..19633
                     /locus_tag="VYO12_000750"
     CDS             18491..19633
                     /locus_tag="VYO12_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1587394.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHSLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDGVQALTKIPTEKYLTERVDFATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19642..20856
                     /gene="thiI"
                     /locus_tag="VYO12_000751"
     CDS             19642..20856
                     /gene="thiI"
                     /locus_tag="VYO12_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1587395.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            20991..21796
                     /locus_tag="VYO12_000752"
                     /pseudo
     CDS             20991..21796
                     /locus_tag="VYO12_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            21818..22557
                     /locus_tag="VYO12_000753"
                     /pseudo
     CDS             21818..22557
                     /locus_tag="VYO12_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            22570..23736
                     /locus_tag="VYO12_000754"
     CDS             22570..23736
                     /locus_tag="VYO12_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1587396.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(24227..25897)
                     /locus_tag="VYO12_000755"
     CDS             complement(24227..25897)
                     /locus_tag="VYO12_000755"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1587397.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            26127..26816
                     /gene="coaB"
                     /locus_tag="VYO12_000756"
     CDS             26127..26816
                     /gene="coaB"
                     /locus_tag="VYO12_000756"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1587398.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            26828..27379
                     /gene="coaC"
                     /locus_tag="VYO12_000757"
     CDS             26828..27379
                     /gene="coaC"
                     /locus_tag="VYO12_000757"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1587399.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETLDEKTL"
     gene            27363..27926
                     /locus_tag="VYO12_000758"
     CDS             27363..27926
                     /locus_tag="VYO12_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1587400.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT         8236 a         5383 c         6050 g         8339 t
ORIGIN      
        1 ccaaaatcct ttgtttacta aacaagggat ttttcttttg tctctgctcc ttttttgata
       61 taatagttct atgttaaaat cagaaaaaca atcacgttat caaatgttaa atgaagaatt
      121 gtccttccta ttggaaggcg aaaccaatgt tttggctaat ctttccaacg ccagtgctct
      181 cataaaatca cgttttccta ataccgtatt tgcaggcttt tatttgttcg atggaaagga
      241 attggtttta ggccccttcc aaggaggtgt ttcctgcatc cgtattgcac taggcaaggg
      301 ggtttgtggt gaggcagctc actttcagga aactgttatt gttggagatg tgacgaccta
      361 tctcaactat atttcttgtg atagtctagc taaaagtgaa attgtggtgc cgatgatgaa
      421 gaatggtcag ttacttggag ttctggatct ggattcttca gagattgagg attacgatgc
      481 tatggatcga gattatttgg aacaatttgt cgctattttg cttgaaaaga caacatggga
      541 ctttacgatg tttgaggaaa aatcttaatg tatcaagcac tttatcgaaa atatagaagt
      601 caaaacttct cccagttagt tggtcaagaa gttgtggcta agactcttaa acaagcggtg
      661 gagcaagaga aaataagtca cgcttatctt ttttctggtc ctcgtggaac gggaaaaacc
      721 agtgttgcta aaatctttgc caaggctatg aactgtccca atcaagtggg tggcgaacct
      781 tgcaataact gctatatttg tcaagcagtg acggacggta gtttagaaga tgtcattgaa
      841 atggatgcag cttctaataa tggggtagat gaaattcgcg aaattcgtga taaatctacc
      901 tatgcgccta gccttgctcg ttataaggtt tatatcatag atgaggttca catgctgtct
      961 acaggggctt ttaatgccct cctaaagacg ttggaagaac caacacagaa tgtagtcttt
     1021 attttggcca ctactgaatt gcacaagatt cctgctacta ttctatcccg tgtgcaacgt
     1081 tttgagttta aatcaattaa gacacaggat attaaggaac atattcacta tatcttagaa
     1141 aaagaaaata tcagttctga accagaggct gtggaaatca ttgccagacg ggcggaaggt
     1201 ggaatgcggg acgccttgtc tattttggat caagccctga gtttgacaca gggaaatgag
     1261 ctgacgactg ctatctctga agaaattact ggcaccatta gcctatcagc cttggatgat
     1321 tatgtggcgg ccttgtctca acaggatgtt cccaaagctt tgtcttgctt gaatcttctt
     1381 tttgacaatg gtaagagcat gactcgtttt gtgaccgatc ttttgcacta tttaagagac
     1441 ttgttaattg ttcaaacagg gggagaaaat actcatcata gttcagtctt tgtagaaaat
     1501 ttggcacttc ctcaaaaaaa tctgtttgaa atgattcgct tagcaacagt gaatttagca
     1561 gatattaagt ctagtttgca gcccaagatt tatgctgaaa tgatgaccgt ccgtttggcg
     1621 gaaatcaagc ccgaaccagc tctatcagga gcggttgaaa atgaaattgc tacgctgaga
     1681 caggaagttg cccgtctcaa acaagagctt tctaatgcag gtgcggttcc taaacaagtt
     1741 gcaccagctc ctagtcgacc agctacgggc aaaacagtct atcgtgtcga tcgcaataaa
     1801 gtgcaatcta tcttacaaga ggccgtcgaa aatcctgatt tagcacgtca aaatttaatt
     1861 cgtttgcaga atgcctgggg agaggtaatt gaaagtctag gtgggccgga caaggctctg
     1921 ctagctggtt ctcaaccggt tgctgccaat gaacaccatg ctattcttgc ttttgagtct
     1981 aacttcaatg ctggtcaaac tatgaaacga gacaatctca ataccatgtt tggtaatatc
     2041 ctcagtcagg cggcaggttt ttcacctgag attttagcta tttccatgga ggaatggaaa
     2101 gaagttcgcg cagccttttc agccaaagcc aaatcttctc aaactgaaaa agaagtagaa
     2161 gaaagcctga ttccagaagg atttgaattt ttggctgata aagtgaaggt agaggaagac
     2221 taaagaaaga tttcatgata caataagttt atgaataaac aacaatttat tattatggcg
     2281 ctatttacag ctgctgagac ctattttttc aatgaagcct ggatgactgg ccgctatatt
     2341 atggcagcct tttgggcaat tttactcttt agaaatttcc gagtcagtta tgtgatgggt
     2401 aaaatcgttg atgtcatcga tcagcatttt aataggaaag actagccctc agcttccaga
     2461 caaaatcaaa gccttttagg cttttttttg ttatactaga aaagtatatt tatagaattt
     2521 ttgctctatt tctggggaaa tcagacgttt ttctagtaag tactgtaaaa gttttgaaaa
     2581 agaaaggaac tatcatgtca gtaatagaga tcaaagatct tcacgttgag attgaaggaa
     2641 aagaaatttt aaaaggggtt aacctgaccc tgaaaacagg agaaattgcc gctatcatgg
     2701 gaccaaatgg tacaggtaaa tcgactcttt ctgccgctat catgggaaat ccaaactatg
     2761 aagtaactaa aggtgaagtt ttgtttgatg gcgtaaacat ccttgagttg gaagtggatg
     2821 agcgtgcgcg tatgggactt ttccttgcta tgcaataccc atcagaaatc cctggaatta
     2881 ccaatgctga atttcttcgt gccgctatga atgcgggtaa agaagatgat gagaagattt
     2941 cagttcgtga gtttattact aagctagatg aaaaaatgga attgcttaac atgaaagaag
     3001 aaatggcaga gcgttacctc aacgaaggct tctctggtgg tgagaaaaaa cgcaatgaaa
     3061 ttcttcaact tttgatgttg gagccaacat ttgctctttt ggacgagatt gactcaggtc
     3121 ttgatattga cgctcttaaa gttgtgtcta aaggtgtcaa tgccatgcgt ggtgaaggtt
     3181 ttggtgctat gatcatcact cactaccaac gtcttttgaa ctatatcaca cctgatgtgg
     3241 tacacgtgat gatggaaggt cgtgttgtcc tttctggtgg tccagaattg gctgcgcgtt
     3301 tggaacgtga aggatacgca aaattagctg aagaacttgg ctacgactac aaggaagaat
     3361 tgtaattccc tcgtatcttt taggagaagt aaatgactag agaaaatatt aaactttttt
     3421 cagaaatgca cgctgaacca agctggttgg ctgatctccg tcaaaaagct tttgacaaga
     3481 ttgagacttt ggaattacca gttattgagc gtgtcaaatt ccaccgttgg aatctgggtg
     3541 atggaacgat tacagaaaat gagccatcag caaatgttcc agatttcaca gctttagatc
     3601 atcacttgaa gttggtgcaa gtaggaactc aaactgtttt cgaacaaact ccagttgagt
     3661 tagctgaaca gggtgttgtc ttcacagact ttcactcagc tttagaagaa attccagagc
     3721 tgatcgaaga attcttcatg tcatctgtta agtatgatga tgacaagttg gcggcttacc
     3781 acacagctta ctttaacagt ggtgctgtac tctatattcc agataacgta gaaatcacag
     3841 agccaattga aggaattttc taccaagata gcgatagcaa tgtgccgttt aacaagcata
     3901 ttatgattat cgctggtaaa aattctaaga ttaattatct ggagcgttta gagtcacgcg
     3961 gtgaaggaag tgccaaagca actgctaata tcacagtaga agtgattgca cgttcaggtg
     4021 cgcaagtcaa gtttgctgct atcgaccgac taggtgaaaa cgtcactgcc tacattagcc
     4081 gtcgtggtaa attaggcaac gatgcaagta ttgactgggc tatcggtgtc atgaacgaag
     4141 gaaatgtcgt tgctgatttt gatagtgact tgattggtaa tggtagccat gctgacctca
     4201 aggttgtagc tctttcaagt ggtcgtcagg tacaagggat tgatactcgt gtaactaact
     4261 atggctgcaa ctctatcgga aatatcctac aacatggggt tatccttgaa aaagcaactt
     4321 tgactttcaa tggtatcggc cacatcatca agggtgctaa gggagcagat gcccaacaag
     4381 agagccgtgt tctcatgctt tcagaccaag cgcgttcaga tgctaaccca attcttttga
     4441 ttgatgaaaa tgatgtcact gcaggacacg cagcgtctat cggacaagtt gatccagaag
     4501 acatgtatta cctcatgagt cgtggattgg ataaggcaac tgcagagcgt ttggttgttc
     4561 gtggtttcct tggatctgtt atcgtggaga ttccagtcaa ggaagttcgt gatgaaatga
     4621 ttgcaactat cgaagagaaa ttgtcaaaac gctaaggggc agcctatgtt agatgtagaa
     4681 gcgattcgca aggattttcc aattttagat cagattgtca atgatgaacc tctggtctat
     4741 ctggacaatg ctgcgacgac acaaaaacca ctagtagttc tgaaagctat taacagctac
     4801 tatgagcagg acaatgccaa tgttcaccgt ggtgtccata ccttagcgga acgagcgaca
     4861 gcttcttatg aagctgctcg tgaaaccatt cgtaagttta ttaatgcagg ctctacaaag
     4921 gaagttctct ttaccagagg aacgacaacc agccttaact gggtggcacg ctttgctgag
     4981 gaaattctca ctgagggaga ccaggtcttg atttcagtaa tggaacacca ttctaatatc
     5041 attccatggc aggaagcttg tcgaaagact ggagcagagc ttgtctatgt ctatcttaaa
     5101 gacggtgcct tggatatgga ggatttgcga gctaaattga ctgataaggt taaatttgtt
     5161 tccctagctc atgcctccaa tgttcttggt gtggtcaatc cgatcaagga aatcactcaa
     5221 ttagcccacc aagttggggc aatcatggta gtggatggtg ctcaatctac acctcatatg
     5281 aagattgatg tccaggactt ggatctggac tttttcgcct tttcgggtca caagatggct
     5341 ggtccgactg gtatcggtgt cctttacggc aaagaaaagt atcttgagca aatgtctcca
     5401 gtagaatttg gcggcgagat gattgatttt gtctacgagc aatctgctag ttggaaggaa
     5461 ttgccttgga aatttgaggc tggaacgcca aatatggcag gagctattgg acttgcgact
     5521 gcagttgatt atctggaaaa gattggtatg gatgccattg aagctcatga acaggaattg
     5581 attgcgtacg tctatccaaa actgcaggca attgagggat tgaccattta cggttctcag
     5641 gatttggctc aacgttcggg tgttattgcc tttaacctag gtgatctcca tcctcacgat
     5701 cttgcgacgg ctctggatta tgaaggagtg gctgttcgtg ctggtcacca ttgtgcgcaa
     5761 cccttgcttc agtatttgga agtcccagca atagctcgtg caagttttta tatctacaat
     5821 accaaggcag attgcgacaa actagtcgat gccctacaaa agacaaagga gtttttcaat
     5881 ggcactttct aaactagata gcctttatat ggcagtggta gcagaccatt cgaaaaatcc
     5941 acatcaccaa gggaagttag aagacgctga gcaaatcagt ctcaacaatc cgacttgtgg
     6001 ggatgtcatc aacctctctg tcaagtttga tgcagaggac cgtttggaag atattgcttt
     6061 tctaaattca ggatgcacga tttcaactgc ttctgctagt atgatgacag atgccgtttt
     6121 aggaaaaacc aaacaagaaa ttttagaact ggcgactatt ttttctgaaa tggttcaagg
     6181 gcaaaaagat gagcgtcaag accaacttgg agacgcggca ttcttgtcag gtgttgccaa
     6241 attccctcaa agaatcaagt gtgcaaccct agcttggaat gcccttaaga aaacaattga
     6301 aaatcaagaa aaacagtaag acaagtttct tttgtcttat gaattattag aaatgaagaa
     6361 agaaaggata ttatggctga agaaagagta gaaccaaaac caattgacct tggtgaatat
     6421 aaatttggtt tccatgacga tgtagagcct gtcttatcga caggaaaagg actcaacgaa
     6481 ggtgttattt gtgaattatc tgctgctaag ggtgagcctg agtggatgtt ggagttccgt
     6541 ttgaagtctt atgaaacctt caaaaaaatg cccatgcaaa cttggggagc agacttgtca
     6601 gagattgact ttgatgactt aatctactac caaaaaccat ctgacaaacc agcccgttct
     6661 tgggatgatg tacctgaaaa gattaaagaa acctttgaac gtatcgggat tccagaagct
     6721 gaacgtgctt atttagcagg ggcttctgcc cagtacgagt cagaagtggt ttaccacaac
     6781 atgaaggaag agttccaaaa attaggtatt atctttacag atacagattc cgcactcaag
     6841 gaatacccag acttatttaa acaatacttt gcgaagttgg taccgccgac agataacaag
     6901 ttggcagccc tcaactcagc agtatggtcg ggtggaactt ttatctacgt gccaaaaggt
     6961 gtcaaggtag atattccact tcaaacttat ttccgtatca ataacgaaaa tataggtcag
     7021 ttcgaacgta ccttgattat cgttgatgag ggagcaagcg tccactacgt agaaggatgt
     7081 acagcaccaa catattcaag caatagctta cacgctgcca ttgtagaaat ttttgctttg
     7141 gacggagctt atatgcgtta tacaactatc caaaactggt ctgataacgt ctataacttg
     7201 gtaacaaagc gtgctaaggc tcaaaaggat gccactgttg agtggattga tggaaacttg
     7261 ggtgccaaaa cgactatgaa atatccatct gtttaccttg atggagaagg agcgcgtggt
     7321 accatgctct ctatcgcctt tgctaatgca gggcaacacc aagacacggg tgctaagatg
     7381 attcacaatg ctccacatac cagctcgtct attgtgtcta aatccatcgc taaaggtgga
     7441 ggaaaggttg actaccgtgg acaagtcacc tttaacaaga actctaagaa atctgtttcc
     7501 cacattgaat gtgataccat tatcatggat gacttgtcag catcagatac tattccattt
     7561 aatgaaattc acaactcgca agtggctttg gaacacgaag ccaaagtatc taagatttca
     7621 gaagagcaat tgtattatct catgagccgt ggattgtcag aatctgaggc aactgaaatg
     7681 attgtcatgg gatttgtaga accctttaca aaagaacttc caatggaata cgcagttgag
     7741 ctgaaccgct tgattagcta tgaaatggag ggatcagttg gataaaattt gattttatgc
     7801 tcttcgaaaa tctcttcaaa ccacgtcagc atcgccttac cgtatgtatg gttactgact
     7861 tcgtcagttt catctacaac ctcaaaacag tgttttgagc aacctgcggc tagcttccta
     7921 gtttgttctt tgattttgag tattagattt actcaaaatc aaggattttg aagatgaact
     7981 tgtatcaaaa aatcgcggtt taaaatcgcg attttttata atttctcgtt aacaaagcgg
     8041 acaaactgat tccaccaaac ttttaagaag aaggcttttt caattttctt gtctgctacc
     8101 atttcgaaac tagggcgctc tgtggtgatg taaccttgac caatcaagtc cttgtcttca
     8161 taagtcaaat ggccaaccac tgttccagct tcaagtggtg ctgggattgc tttggaatca
     8221 ggtgtgaatt gaacagattg ggaagattga ttcccaacac gttcgattag atagatatcc
     8281 tctggagcca ctgcagttac tgtatcttct tttccatctt gtacaggggc tttgctatct
     8341 tgataggcat cgccttgttg aacgattttg cgaagtgtaa atgtagaaga aatataatcc
     8401 attagggaag atgtagctgt aaatcgagcg taaggattat tgtcttgatg atctgcattt
     8461 aaaacaactg tgatgactct catgcctttt tcgacagtag taccaacaaa agactctcca
     8521 gccttatctg ttgttcctgt ttttagccca tcaaaaccac cacggtaagc aggcatacct
     8581 tctaacatgt agttggttga agtgattgtc atcccagcaa aagtagaaga tggttttttg
     8641 gtgatttcta agacttgtgg gtattttttg atgaggttgc gagcaacgat agcgacatca
     8701 taagcactaa gcttattttc ctcatctttt ttagaacctg ggtaaatgtt atcccctaga
     8761 gtttcattgt taagacctgt cgtattgaca acagtggcat cctgaattcc ccattccaag
     8821 agttttgccc gcatcatatc gacgaaatct ttttctgagc cagcaatttt ctcagctagg
     8881 gcaatagcgg cgctgttggc actagatacc agagttgctt caagcaactc ttcgacagta
     8941 taattacggg cctccatagg aatattactg gcttcagaat ttgtcgtcaa ttgataagga
     9001 taatcagaaa tatctacagg agtggagagg gtaatacttc cgttttccaa agcttcatag
     9061 accagataaa cagtaatcaa ttttgttatg gaagcaattt cgacaggttg cgttgcatcc
     9121 ttctcataga gaattttacc agtatttgcc tcaacagcaa tcgcatgttt agcggcaatg
     9181 gtaaaatctt gagcaacagc agtagaagca ccccctaaaa gagagacagt taacaaagtt
     9241 aaaaatattt ttttcatagt agtcttattc tatcataaag aaaaaaaata ttcttgcttt
     9301 aataattcat ctgttaagct ttttgaaaat atggtaaaat aaagtgaggg aggtaactca
     9361 tgtttcgtag aaataaatta tttttttgga ccacagaaat tttactctta accatcatct
     9421 tttacctatg gagacagatg gggtctttga ttaacccttt tgttagcgtg cttaatacaa
     9481 ttatgattcc atttttatta gggggctttc tttattattt gacaaaccct attgttactt
     9541 tcttaaataa agtctgtaaa ctcaatcgtt tgcttggtat tttaattacc ttgtgtactt
     9601 tggtctgggg aatggtcata ggtgttgtct atctcttacc tattttgatt aatcagttat
     9661 ctagtttgat tatatctagt caaactattt atagtcgagt acaagactta atcatagact
     9721 tatctaatta tcctgcgctc cagaatttgg atgtagaagc tacaattcag cagttaaact
     9781 tatcctatgt tgatattctt caaaatatcc taaatagcgt atcaaatagt gtggggagcg
     9841 tcttgtcagc tcttatcagt actgttttga ttttgattat gactccagtt tttttggttt
     9901 atttcttatt agatggacat aaattcttgc ccatgcttga aagaacgatt ctaaagaggg
     9961 atcgcttgca tattgcaggc ttattaaaga atttaaatgc gacgattgct cgctatatta
    10021 gtggagtttc gattgacgca atcattatag gttgtttggc ttatattggc tatagtatta
    10081 ttggtttaaa atatgcttta gtttttgcca ttttttctgg tgtagccaat ttaattcctt
    10141 atgtggggcc aagtattggt ttgattccta tgatcatcgc aaatatattc actgatcccc
    10201 atagactgct gattgcagtg atttatatgc ttgttgttca gcaggtagat ggcaatatct
    10261 tatatcctcg aattgtagga agtgttatga aggttcatcc aatcacgatt ttagttttac
    10321 ttttgttgtc aagcaatatc tatggtgtag ttggaatgat tgtcgcagtg ccaacctatt
    10381 ctatcttgaa agaaatttct aagttcttat cccgtttgta tgaaaatcat aaaataatga
    10441 aagaacgaga aagagaatta gctaagtaaa agtcaggaga accctgattt ttctttactg
    10501 gaagtggcct ttagattaga agactgaaaa taagttaaag tcttaaacta attttcacag
    10561 ctaagaatag tagaagttaa tctgataaaa atcgaaaaaa ccagtggaat tccgtgtcag
    10621 ggtaagttcc actggttttc atagtctatt aaagttcgaa tgaaacctat ttatagtata
    10681 ttgaaactag aatagtacac ctctaattct aaaacattgt tagaaatcga tttgactgtc
    10741 ctgatctatt cgtcctattc ttatttcatt ttactatatt ttggtgcaat aagtgaaaag
    10801 tagtccgaat aatataagga ttgattttat agtttttaaa ctcaaatgaa ttaaaataaa
    10861 gagagtacga aaattctcat ctgaaagtat tttagaataa ttttcttcgt gaatttcttc
    10921 aaaacagata gcttcatctt aggtatgtgt taattggatt tcgtaagctc tatttacaat
    10981 cgtaaagtta tgcactgaga aagttgtagt tagtcttcca gtttgctaaa tttctcgaat
    11041 agtatctttt ttaggtatgt gatttctttt tgcatttttg agttagataa ggtataatgg
    11101 ttttattgtc ttttggggtc gttacggatt cgacaggcat tatgaggcat attttgcgac
    11161 tcgtgtggcg acgtaaacgc tcagttaaat ataactgcaa aaaataacac ttcttacgct
    11221 ctagctgcct aaaaaccagc aggcgtgacc cgatttggat tgctcgtgtt caatgacagg
    11281 tcttattatt agcgagatac gattaagcct tgtctagcgg cttgataaga gattgataga
    11341 ctcgcagttt ctagacttga gttatgtgtc gaggggctgt taaaataata cataacctat
    11401 ggttgtagac aaatatgttg gcaggtgttt ggacgtgggt tcgactccca ccggctccat
    11461 tattcctttg cattcttttg cattccttgg taaaacgttg ttaaatcaac gttttttatt
    11521 tttatctttg gtattccttt gcattctttt gctaaaaagg gagtcacaaa cagaccctat
    11581 tttaaaaaaa ggatagaaaa aaggatacaa catttgtcgc atcctaaaaa taatcttttt
    11641 tccacggaag acatgggatt cgaacccacg cacgctatta cacgcctacc gcgtttccaa
    11701 cacggcctct taagcctctt gagtaatctt ccaatactta ctcaaatagt ctaccataaa
    11761 ggctcttatc ttgcaataaa aattctagaa atatgaaaaa tgatagattt tgaaagaaaa
    11821 tgataaaaaa tgcttgactt cgaaagaaag tatgatagaa tgaatagtgt aaacgataac
    11881 aggaggtgat tcagtgttaa aaacagaacg taaacaacta attttagagg agttaaatca
    11941 acatcatgta gtttctctag aaaaattagt tagtttgcta gaaacgtcag aatcaacggt
    12001 tcgaagagac ttggatgagt tggaagcgga aaacaagctt cgtcgtgtgc atggtggagc
    12061 agaactcccc cactccttac aggaagaaga aaccattcaa gaaaaatatg tcaaaaacct
    12121 tcaagaaaag aagttgctgg ctcagaaagc agcctctctc attaaagaaa aagatgtcat
    12181 ctttatcgat gctggaacaa caactgcttt tttgattcat gaattggtca ataagaatgt
    12241 tacagttgtg accaactcca ttcaccatgc cgctcagttg gttgaaaagc agattccagc
    12301 tgtcatggtt ggaggaaacg tcaagacggc gacagatgct agtatcgggg gcgttgctct
    12361 taaccagatt aaccaattgc actttgaccg tgcctttatc ggaatgaatg gtgttgacga
    12421 tggctattat acgactcctg atatggagga gggagctgtg aaaagagcta ttttggagaa
    12481 tgccaagcag acctacgtct tggtggattc gtcaaaaatt ggacaaactt gctttgccaa
    12541 ggtagcacca ctcaaacgcg ctatcgttat cactagtcaa gggcatgagc tcttgcaggt
    12601 tattaaggag aaaacggagg taatagaagt atgatttata cagtcacact caatccatcc
    12661 attgactata tcgttcgttt ggaccaagtc aaagttggta gtgtaaatcg tatggacagt
    12721 gatgataagt ttgctggtgg gaaaggaatc aatgtcagcc gtgtcttgaa acgtttgaat
    12781 ataccaaata cagcgacggg atttatcggt ggctttactg gtaaatttat cacagatact
    12841 ttagcagagg aagaaatcga gacacgtttt gtccaggtgg cagaagatac tcgtatcaat
    12901 gttaaaatca aagcagacca agaaacagaa atcaacggaa cgggtccaac tgttgaatcg
    12961 gttcagctag aagaattgaa agctatttta tctagtctga cagcagaaga tacagttgtc
    13021 tttgcaggtt caagtgctaa aaatctaggc aatgttatct ataaggattt gatttccttg
    13081 acgcgccaga ctggtgcgca agtggtctgt gactttgaag gacagacctt aattgatagt
    13141 ttggactacc agcctcttct tgtaaaacca aacaatcatg aacttggagc gatttttggg
    13201 gttaaactcg aaagtttaga tgaaattgag aaatacgctc gtgagttact ggctaagggt
    13261 gctcaaaatg ttattatctc tatggctggg gacggtgctc ttcttgtcac atctgaggga
    13321 gcttactttg ctaaaccaat caaaggaaca gtcaaaaatt cagttggggc tggtgattct
    13381 atggttgctg gattcacagg tgaatttgtc aaatcaaaag acgcagtaga agccttcaaa
    13441 tggggagtgg cttgcggaac ggcaactacc ttctcagatg acttggcaac ggcagaactt
    13501 attaaagaaa catatggaaa agttgaggta gaaaaacgat gaaaattcaa gacctattga
    13561 gaaaagatgt catgttgcta gatttgcagg caactgaaaa aacagctgtc atcgacgaga
    13621 tgattaaaaa tttgacagac cacggttatg taacagattt tgaaacattt aaagaaggaa
    13681 ttttggcgcg tgaagctttg acttctactg gtttgggtga tggaatcgca atgcctcaca
    13741 gcaaaaacgc tgctgtcaaa gaagcgacag ttctatttgc taagtcaaat aagggtgttg
    13801 actacgagag cttggatgga caagcaactg acctcttctt catgattgca gctccagaag
    13861 gtgccaatga tactcacttg gcagccttgg cagaattgtc tcaatacttg atgaaagacg
    13921 gttttgcaga caaacttcgt caagcaacat ctgcagacca agttatcgaa ctttttgacc
    13981 aagcttcaga aaaaactgag gaacttgttc aagcacctgc taatgactct ggtgacttta
    14041 tcgtagctgt tacagcttgt acaacaggta ttgcccacac ttacatggcc caagaagccc
    14101 ttcaaaaagt agctgctgaa atgggggttg gtatcaaggt cgaaaccaac ggtgctagcg
    14161 gtgttggaaa tcaactaact gcagaagata tccgtaaggc taaagctatt atcattgcag
    14221 cagacaaggc cgttgaaatg gatcgatttg atggaaaacc attgatcaat cgtccagttg
    14281 ctgacggtat ccgtaagaca gaagagctaa ttaacttggc tctttcagga gatactgaag
    14341 tctaccgtgc cgctaatggt gcaaaagctg caacagcctc taacgaaaaa caaagccttg
    14401 gtggtgcctt ctacaaacac ttgatgagtg gtgtatctca aatgttacca ttcgttatcg
    14461 gtggtggtat catgattgcc cttgccttct tgattgacgg tgctttgagt gttccaaatg
    14521 aaaaccttgg caatcttggt tcttaccatg agttagcttc tatgttcatg aaaattggtg
    14581 gagctgcctt tggtttgatg cttccagtct ttgcgggtta tgttgcctac tctattgctg
    14641 aaaaaccagg tttggtagca ggtttcgtgg ctggtgctat tgccaaagaa ggttttgcct
    14701 ttggtaaaat tccttatgtc gcaggtggtg aagcaacttc aactcttgca ggtgtctcat
    14761 ctggtttcct aggtgccctt gttggtggat ttatcgcagg tgccttggtt cttgccatca
    14821 agaaatacgt taaagttcct cgttcactcg aaggtgctaa atcaatcctt ctattgccac
    14881 ttcttggaac aatcttgaca ggatttgtta tgctagctgt gaatatccca atggctgcaa
    14941 tcaacactgc tatgaatgac ttcctaggtg gtcttggagg aggttcagct gtccttcttg
    15001 gtatcgtcct tggtggaatg atggctgttg acatgggtgg accagttaat aaagcagctt
    15061 atgtctttgg tacaggtacg cttgcagcaa ctgtttcttc aggtggttct gtagccatgg
    15121 cagcagttat ggctggagga atggtgccac cacttgcaat ctttgtcgca actcttcttt
    15181 tcaaagataa atttactaag gaagaacgta actctggttt gacaaacatc atcatgggct
    15241 tgtcatttat cactgaggga gcgattccat ttggtgccgc tgacccagct cgtgcgattc
    15301 caagcttcat ccttggttca gcagtagcag gtggactcgt tggtcttact ggtatcaaac
    15361 tcatggcgcc acacggagga atcttcgtta tcgcccttac ttcaaatgct ctcctttacc
    15421 tcgtttctgt cttggtagga gcaatcgtaa gtggtgtggt ttatggttac ctacgcaaac
    15481 cacaagcata aaaaatagaa aaatgaaaag attggaccgt ttggtgcagt ctttttctct
    15541 tcccgaaatg cctgtgaaat atggtataat agaagaatgg caaacaagaa tacaagtaca
    15601 acaagacgga gaccgtctaa agcagaactg gaaagaaaag aagcgattca acgaatgttg
    15661 atttcgttag gaattgcgat tttattgatt ttcgcagcct tcaaattagg ggctgcaggt
    15721 ataacccttt ataatttaat tcgcttgcta gtgggtagcc tagcttatct ggcgatattc
    15781 ggcctattaa tctatctctt ctttttcaag tggatacgaa aacaggaagg actcttatct
    15841 ggttttttca ccatatttgc tggcttactc ttgatttttg aggcctactt ggtttggaaa
    15901 tatggtttgg acaagtccgt tctaaaaggg accatggctc aggttgtgac agatctgact
    15961 ggttttcgaa cgactagctt tgctggaggg ggcttgatcg gggtcgctct ttatattcca
    16021 acagcctttc tcttttcaaa tatcggaact tactttattg gttctatctt gattttagtg
    16081 ggttctctcc tagtcagccc ttggtctgtt tacgatattg ctgaattttt cagtagaggc
    16141 tttgccaaat ggtgggaagg gcacgagcgt cgaaaagagg aacgctttgt caaacaagaa
    16201 gaaaaagctc gccaaaaggc tgagaaagag gctagattag aacaagaaga gactgaaaaa
    16261 gccttactcg atttgcctcc tgttgatatg gaaacgggtg aaattctgac agaggaagct
    16321 gttcaaaatc ttccacctat tccagaagaa aagtgggtgg aaccagaaat catcctgcct
    16381 caagctgaac ttaaattccc tgaacaggaa gatgactcag atgacgaaga tgttcaggtc
    16441 gatttttcag ccaaagaagc ccttgaatac aaacttccaa gcttacaact ctttgcacca
    16501 gataaaccaa aagatcagtc taaagagaag aaaattgtca gagaaaatat caaaatctta
    16561 gaagcaacct ttgctagctt tggtattaag gtaacagttg aacgggccga aattgggtca
    16621 tcagtgacca agtatgaagt caagccggct gttggtgtaa gggtcaaccg catttccaat
    16681 ctatcagatg acctcgctct agccttggct gccaaagatg tccggattga agcaccaatc
    16741 cctgggaaat ccctaatcgg aattgaagtg cccaactccg atattgccac tgtatctttc
    16801 cgagaactat gggaacaatc gcaaacgaaa gcagaaaatt tcttggaaat tcctttaggg
    16861 aaggctgtta atggaaccgc aagagctttt gacctttcta aaatgcccca cttgctagtt
    16921 gcaggttcaa cgggttcagg gaagtcagta gcagttaacg gcattattgc tagcattctc
    16981 atgaaggcga gaccagatca agttaaattt atgatggtcg atcccaagat ggttgagtta
    17041 tctgtttaca atgatattcc ccacctcttg attccagtcg tgaccaatcc acgtaaagcc
    17101 agcaaggctc tgcaaaaggt tgtggatgaa atggaaaacc gttatgaact ctttgccaag
    17161 gtgggagttc ggaatattgc aggttttaat gccaaggtag aagagttcaa ttcccagtct
    17221 gagtacaagc aaattccgct accattcatt gtcgtgattg tggatgagtt ggctgacctc
    17281 atgatggtgg ccagcaagga agtggaagat gctatcatcc gtcttgggca gaaggcgcgt
    17341 gctgcaggta tccacatgat tcttgcaact cagcgtccat ctgttgatgt catctctggt
    17401 ttgattaagg ccaatgttcc atctcgtgta gcatttgcgg tttcatcagg aacagactcc
    17461 cgtacgattt tggatgaaaa tggagcagaa aaacttcttg gtcgaggaga catgctcttt
    17521 aaaccgattg atgaaaatca tccagttcgt ctccaaggct cctttatctc ggatgacgat
    17581 gttgagcgca ttgtgaactt catcaaggct caggcagatg cagactacga tgagagtttt
    17641 gatccaggtg aggtttctga aaatgaagga gaattttcgg atggagatgc tggtggtgat
    17701 ccgctttttg aagaagctaa gtctttggtt atcgaaacac agaaagccag tgcgtctatg
    17761 attcagcgtc gtttatcagt tggatttaac cgtgcgaccc gtctcatgga agaactggag
    17821 atagcaggtg tcatcggtcc agctgaaggt accaaacctc gaaaagtgtt acaacaataa
    17881 aaaaatagct tctttccgag tttggaggga agctatttta gtggctattg attgctttta
    17941 ttttctgaag ttggcgcatt ggactgtttt tcgttttcag tagcaggttt acttgaagca
    18001 ggagtagaag agtcctgagt tgctgttttc tgatcttctt ttttctcttc cttgacgcta
    18061 gattttggtg tttcctcttg ctgtgttttt tcttgactag tgttagtctc tttagttgga
    18121 ctggtgtttt ccttagggga ttccttttgg atttctttga caatggttgt cgtctggctt
    18181 gtcgtaggtt cttttttaat atttttgtta ttatccaagg cgttcataat cgtgatactt
    18241 gcggtaatca agccaaggat actagcgatt gtagcaacgg ttttttgaaa gactgtaaag
    18301 ccttttttga tgtgttctgt ttttggtttt gaagttctac gatagttaga catacattct
    18361 ctcctttgat tatgatttat tatactctgt ttctttaaaa aaatcttaaa aaaagagaaa
    18421 ttgccctttc ttgtcgcaag ctccgtttca tgatacaata gaggaagtga ttttagaaag
    18481 cgtgagaaac atgatttact ttgataattc agcgacgacc aagccttatc ctgaagccct
    18541 tgaaacctat atgcaggtcg cttcaaaaat tttaggaaac ccatctagtc tccatagttt
    18601 gggagaccaa gcaacacgaa tcttagatgc ttctcgccaa cagattgcag atttaatcgg
    18661 taagaaaagc gatgaaatct tctttacctc tggtggaaca gaaggggata actgggttat
    18721 caagggtgtg gcctttgaaa aagctcagtt tggcaagcac atcattgttt cagccattga
    18781 acatccagca gtcaaagagt cagccctctg gttgaaaagt caaggatttg aagtggattt
    18841 tgctccagtt gataagaaag gcttggtcga tgttgaggcg ttagcaggtt tgatacggcc
    18901 tgatacaatc ctcgtttcta tcatggctgt gaacaatgaa atcggctcta tccaacctat
    18961 tgaggctatt tcagaattct tggcagacaa gccgactatt tccttccacg ttgatggggt
    19021 tcaggcgctt accaagattc cgactgaaaa gtatctgaca gaacgagtgg attttgcgac
    19081 tttctctagt cacaagttcc acggggttcg aggtgttggc tttgtctata tcaaatctgg
    19141 caagaagatt acacctcttc ttacaggtgg tggccaggag cgagattatc gttcgacaac
    19201 tgaaaatgtg gcagggattg cagcgacagc caaggccctc cgtttgtcta tggaaaagct
    19261 agatatcttt aggagcaaga ctgggcagat gaaggcagtg attcgccaag ctcttctgaa
    19321 ctatccggat atttttgtct tttcagatga ggaagacttt gcacctcata ttctgacttt
    19381 tggaatcaaa ggtgttcgag gtgaagtcat cgttcacgcc tttgaagact atgatatttt
    19441 catctcaaca acctcagctt gttcatctaa ggcaggaaaa ccagccggta ccttgattgc
    19501 catgggagtg gacaaagata aggccaagtc agctgtgcgt cttagcctag acttggaaaa
    19561 tgatatgagt caggtcgagc agtttttgac caagttaaaa ttgatttaca atcaaactag
    19621 aaaagtaaga taggagcatt catgcagtat tcagaaatta tgattcgcta cggagagttg
    19681 tcaaccaagg gtaaaaaccg tatgcgtttc atcaataaac ttcgtaataa tatttcggac
    19741 gttttgtcta tctataccca agttaaggta acagcagatc gcgaccgtgc ccacgcttac
    19801 ctcaatggag ctgattacac agcagttgca gaatctctca aacaagtttt tggaattcaa
    19861 aacttttctc ctgtttataa ggttgaaaaa tctgtagaag ttttgaagtc ttctgtccaa
    19921 gagattatgc gggacatcta caaggaaggt atgaccttta agatttctag caagcgtagc
    19981 gaccacaact ttgaacttga tagtcgtgaa ctcaaccaaa cacttggagg ggctgtattc
    20041 gaagccattc caaatgtgca agttcaaatg aaaagtcctg acatcaatct tcaggtggag
    20101 attcgtgaag aagcagccta tctttcttat gaaaccattc gtggggctgg tggtttgcca
    20161 gttggaactt caggtaaagg gatgctcatg ttgtcaggag ggattgactc acctgtagca
    20221 ggttatcttg ctcttaagcg tggggtggat atcgaggcag ttcactttgc tagtccacca
    20281 tatactagtc ctggtgccct caagaaagcg caggacttga cccgtaaatt gaccaagttt
    20341 ggcggaaata tccagtttat agaggtgcct ttcacagaga ttcaagagga aatcaaagcc
    20401 aaagcgccag aagcttattt gatgactcta actcgtcgct ttatgatgcg gattactgac
    20461 cgtattcgtg aggtacgaaa tggtttggtt atcatcaatg gggaaagtct aggtcaagta
    20521 gccagccaaa cccttgaaag tatgaaggct atcaatgctg ttaccaacac tcccatcatt
    20581 cgtcctgtgg ttaccatgga caagttggaa atcattgaca tcgcccagga aatcgatacc
    20641 tttgacattt caatccaacc gtttgaagac tgttgtacca tttttgcacc agatcgtcca
    20701 aaaacaaatc ctaaaattaa gaatgcggag cagtacgaag cgcgtatgga tgttgaaggc
    20761 ttggttgagc gagcagtggc tggaatcatg attactgaaa tcacacctca agccgaaaaa
    20821 gatgaagttg atgacttgat tgataatcta ctctaattca gaaaatccaa aagaatagcg
    20881 aaaatcagta aaaaaagtta gttttttctc taaaaacagg taaaaaacta acttttttta
    20941 tttttatgat ataatgatat aaaattttga atatagagag ttttctgaca atgaatcaat
    21001 cctactttta tctaaaaatg aaagaacaca aactcaaggt tccttataca ggtaaggagc
    21061 gccgtgtacg tattcttctt cctaaagatt atgagaaaga tacagaccgt tcctatcctg
    21121 ttgtatactt tcatgacggg caaaatgttt ttaatagcaa agagtctttc attggacatt
    21181 catggaagat tatcccagct atcaaacgaa atccggatat cagtcgcatg attgtcgttg
    21241 ctattgacaa tgatggtatg gggcggatga atgagtatgc ggcttggaag ttccaagaat
    21301 ctcctatccc agggcagcag tttggtggta agggtgtgga gtatgctgag tttgtcatgg
    21361 aggtggtcaa gccttttatc gatgagacct atcgtacaaa agcagactgc cagcatacgg
    21421 ctatgattgg ttcctcacta ggaggcaata ttacccagtt tatcggtttg gaataccaag
    21481 accaaattgg ttgcttgggc gttttttcat ctgcaaactg gctccaccaa gaagccttta
    21541 accgctatat cgagtgccag aaactatcgc ctgaccagcg catcttcatc tatgtaggaa
    21601 cagaagaagc agatgataca gacaagacct tgatggatgg caatatcaaa caagcctata
    21661 tcgactcgtc gctttgctat taccatgatt tgatagcagg gggagtacat ctggataatc
    21721 ttgtgccatc catagtgaaa tcccttggtc agaaaatcta ccagattgtc tgagattttt
    21781 tgcagaaaaa tggtaagtta agaaaggaaa aaacgaaatg catattgaac atcttagcca
    21841 ctggagtggt catcctaacc gtgaaatgta ccttaaccgt tatggacatg gtgggattcc
    21901 agttgtggtc tttgcttcat caggtggtag tcacaacgaa tactatgatt ttggcatgat
    21961 tgatgcctgt gcttccttta tcgaggaagg ccgtgtccag ttctttaccc tatctagtgt
    22021 ggatagtgag agctggttgg ctacttggaa aaatgctcat gaccaagcgg aaatgcaccg
    22081 tgcctacgaa cgttatgtga ttgaggaggc cattctttta tcaagcacaa gacaggttgg
    22141 tttgatggca tgatgacgac aggttgctct atgggagcct atcatgcact taatttcttc
    22201 ctccagcatc cagatgtctt taccaaagtg attgctctca gtggtgttta cgacgcacgt
    22261 ttctttgtcg gtgattacta caacgatgat gctatttacc aaaactcgcc agtagattat
    22321 atttggaacc aaaacgacgg ctggtttatt gaccgttacc gtcaggcaga gattgtgctg
    22381 tgtacggggc ttggagcctg ggaacaagat ggtttaccat ccttttacaa gctcaaagaa
    22441 gcctttgacc agaaacaaat tccagcctgg tttgctgaat ggggacatga tgtcgcccac
    22501 gactgggaat ggtggcgtaa acaaatgcct tatttcctcg gtaatctcta tttataaaag
    22561 gagttaccta tgaattacct tgttatttct ccctactatc cacaaaactt tcaacagttt
    22621 accatcgaac tagctaataa aggcatcaca gtcttgggaa ttggtcaaga gtcttacgag
    22681 caattggatg agcccttgcg caatagcttg accgagtatt ttcgtgttga taatcttgag
    22741 aacatagatg aagtcaaacg tgcagttgct tttctctttt ataaacatgg tccaattgac
    22801 cgcatcgagt ctcacaatga atactggctt gagctagacg caacactcag agaacaattc
    22861 aatgtttttg gtgccaaacc agaggatctc aaaaagacga aatataagtc tgaaatgaag
    22921 aaacttttca aaaaagcagg tgttcctgtg gtacctggag ctgttatcaa gacggaagca
    22981 gatgttgatc aagcagtgaa agaaatcggt cttccaatga ttgccaaacc tgataatgga
    23041 gtgggagcag ccgcaacctt taaacttgag acagaagacg atatcaatca cttcaagcaa
    23101 gaatgggacc attcaaccct ttatttcttt gaaaaatttg tcacttccag cgaaatctgt
    23161 acctttgacg ggctcgtgga caaggatgga aagattgtct tctcaacaac ctttgactac
    23221 gcctatacac cgcttgacct catgatttat aagatggaca attcttatta tgtgctcaag
    23281 gatatggatc ctaaactgcg caagtatggg gaagcaattg tcaaagaatt tggtatgaaa
    23341 gaacggtttt tccatattga gttcttccgt gagggggacg attatattac catcgagtac
    23401 aataaccgcc ctgcaggtgg ttttaccatt gatgtttata actttgctca ttccttggac
    23461 ctttatcgtg gctatgcagc tattgtcgca ggagaggagt tcccggcgtc agactttgaa
    23521 actcagtatt gtttggctac ttctcgccgt gcaaatgctc actatgttta ttcagaagag
    23581 gatttgcttg ccaaatatag ccagcagttc aaggttaaaa aagtcatgcc agctgccttc
    23641 gcggaacttc aaggagatta cctgtatatg ctgacaactc cgagtcgaca agaaatggat
    23701 cagatgattg ctgatttcgg acaacgtcaa gaataagaac tatcggatta aggaaattca
    23761 ctcccttaat ccttttgttt tgtctggtaa aaaataagag catcctaaca aggtagctat
    23821 cataaaactt gttcgataac tatttgaagc aggattaggt ggctagaaat tgaattttaa
    23881 catttcaatt gagtcatagt attgtgtttt cgtatcctta aatcagctaa aaggatccat
    23941 gacgacacct atacgatata gttttcagga taccaaacaa gtctattaat actcaatgaa
    24001 aatcaaagag caaactagga agctagccgc aggttgctca aaacactgtt ttgaggttgt
    24061 ggatagaact gacagagtca gtatcatata cctacggcaa ggtgaagctg acgtagtttg
    24121 aagagatttt cgaagagtat aaatcttatt gatgaactgc ttgcagtctg agaaaaaatg
    24181 agcttggata ttatttccaa actcacttaa agtcaatttc aatccactag aacaagccta
    24241 gtacagttcc atcgctttca acatccatgt tgagagctgc tggacgtttt ggaagacctg
    24301 gcatggtcat aacatcacca gttaaggcaa cgatgaagcc tgcacctaat tttggtacca
    24361 attcacgaat ggtaatttca aagttttctg gtgctccaag cgcatttgga ttgtctgaga
    24421 aactgtattg agttttagcc atacagattg gcaatttgtc ccaaccgttt tgaacgattt
    24481 gagcaatttg tgtttgagct ttcttctcaa agttcacttt gctaccacga tagatttcag
    24541 tgacaatttt ttcaatcttt tcttggacag aaaggtcatt atcgtacaaa cgtttatagt
    24601 tagctggatt ttcagcaatt gtcttaacaa ctgtttcggc aagtgctact ccaccttctg
    24661 ctccatcagc ccagacacta gccaattcaa ctggtacatc gattgaggca cagagttctt
    24721 ttaaggctgc aatttcagct tctgtatcag atacaaattc gttaatagct acaactgctg
    24781 gaataccgaa cttacggata ttttcaacgt ggcgtttcaa gttagcaaaa cctgcacgaa
    24841 ctgcctctac attttcttca gtcagagcgt ctttagccac accaccattc atcttaaggg
    24901 cacgaagggt tgcgacaata acaactgcat ctggagatgt tggcaagttt ggtgtcttga
    24961 tatcaaggaa tttctcagca ccaaggtccg caccaaaacc agcttcagta acagtgtaat
    25021 cagccaagtg aagggctgtt gtcgtcgcca aaacagagtt acagccatga gcgatattgg
    25081 caaatggacc accgtgtaca aaggcaggtg taccgtaaat tgtctgaacc aagtttggct
    25141 taatagcatc cttcaaaatc aaagccaagg caccctcaac ctgcaaatca cctacagaaa
    25201 caggcgtacg gtcatagcga taaccaataa cgatattcgc caaacgacgt ttcaagtcct
    25261 cgatgtccgt tgccaagcaa agaattgcca tgatttctga agcaactgta atatcaaaac
    25321 catcctcacg tggaataccg tttagaggac caccaagacc aacagtcaca tggcggagcg
    25381 cacggtcgtt caagtccaca acgcgtttcc agaggatacg acgttgatca atttccagct
    25441 cattcccttg gtgcaagtgg ttgtcaatca aggcagaaag ggcattgttg gcagttgtaa
    25501 tagcatgcat atctccagta aagtggaggt tgatgtcttc cattggcaga acttgtgcat
    25561 acccaccacc agcagcacca cccttgatcc ccatgactgg accaagagac ggttcgcgga
    25621 tagcaatcat ggttttcttg ccaatcttgt tcaaggcatc cgcaagacca atggtaagtg
    25681 tcgactttcc ttcacctgca ggtgttgggt tgatggcagt aaccaagatc aatttaccga
    25741 ctggattgct ctcaactgca cgaattttat caaagctgag tttagccttg tactttccgt
    25801 acaactccaa atcgtcataa gaaataccaa gtttctctac aacatcaaca attggcttca
    25861 actcaatact ctgtgcgatt tcaatatctg ttttcattca aaattcctct aacctcttat
    25921 atgataattc attatatcac aaaacaagat ttttaacatc ctaaaactct ctaaacgttc
    25981 gtaaatatct ctgtttttaa ggcttttaga gtcctttctt aaattttata tggttttata
    26041 gtttgaaact atagtaaatc ttcgttttta ccaaaaattt atcactttca ttttacttac
    26101 cgcttatttt tgtgtacaat agtgctatga aaattttagt tacatcgggc ggtaccagtg
    26161 aagctatcga tagcgtccgc tctatcacta accattctac aggtcacttg gggaaaatta
    26221 tcacagagac tttgcttttt gcagggcatg aagtttgttt aattacgaca aaacgagctc
    26281 tgaagccaga gcctcatcct aacctaagta ttcgagaaat taccaatacc aaggaccttc
    26341 taatagaaat gcaagaacgt gttcaggatt atcaggtctt gatccactca atggctgttt
    26401 ctgactacac tcctgtttat atgacagggc ttgaggaagt tcaggctagc tccaatctaa
    26461 aagaattttt aagcaagcag aatcatcagg ccaagatttc ttcaactgat gaggttcagg
    26521 ttttgttcct taaaaagaca cccaaaatca tatccctagt caaggaatgg aatcctacta
    26581 ttcatctgat tggtttcaaa ctgctggttg atgttaccga agatcatctg gttgacattg
    26641 cacgaaaaag tcttatcaag aatcaagcag atttaatcat cgcgaatgac ctgactcaaa
    26701 tttcagcagg tcagcaccga gctatatttg ttgagaaaaa tcagcttcaa acagtccaga
    26761 ctaaagaaga aattgcagaa ctcctccttg aaaaaattca agcctatcat tcttagaaag
    26821 gaaaactatg gcaaacattc tcttggctgt aacgggttca atcgcctctt ataagtcggc
    26881 agatttagtc agttctctaa aaaaacaagg ccatcaagtc actgtcttaa tgactcaggc
    26941 tgctacagag tttatccaac ctttgacact acaggtactc tcacagaatc ctgtccactt
    27001 ggatgtcatg aaggaaccct atcctgatca ggtcaatcat atcgaacttg gaaaaaaagc
    27061 agatttattt atcgtggtac ctgcaactgc taacactatt gcaaaactag ctcacggatt
    27121 tgcggacaac atggtaacca gtacagctct agccctgcca agtcatattc ccaaactaat
    27181 agctcctgct atgaatacaa aaatgtatga ccatccagta actcagaata atctgaaaac
    27241 attagaaacc tacggctatc agctgattgc tcctaaggaa tccctactag cttgtggaga
    27301 ccacggacga ggagctttag ctgacctcac aattatttta gaaagaataa aggaaactct
    27361 cgatgaaaaa acgctctaat attgcaccca ttgctatctt ttttgctacc atgctcgtga
    27421 tacactttct gagctcactt atctttaacc tttttccatt tccaatcaaa ccgaccattg
    27481 ttcatattcc tgtcattatt gccagcatta tttatggtcc acgagttggg gttacacttg
    27541 gatttttgat ggggctactt agcttgacgg ttaacacgat tacgattcta ccgacaagct
    27601 acctcttctc tcccttcgta ccaaacggaa acatctactc agctatcatt gccatcgtcc
    27661 cacgtatttt gattggttta actccttact tagtctataa actgatgaaa aacaagactg
    27721 gtctgatttt agctggagcc cttggttcct tgacaaatac tatctttgtc cttggaggaa
    27781 tcttcttcct atttggaaat gtttataatg gaaatatcca acttcttctg gcaaccgtta
    27841 tctcaacaaa ttcaattgct gaattggtca tttctgcaat tctaacccta gccattgttc
    27901 cacgactaca aaccttgaaa aaataaaaac aatctccgct ttcaaacctg aaggtggaga
    27961 ttttgctcta tacagtttct ttttaatgaa atctttacta tagtaaaa
//
LOCUS       DAVLXF010000019        27522 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLXF010000019 DAVLXF010000000
VERSION     DAVLXF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.019"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            437..670
                     /locus_tag="VYO12_000759"
     CDS             437..670
                     /locus_tag="VYO12_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1587401.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            953..2902
                     /gene="mutL"
                     /locus_tag="VYO12_000760"
     CDS             953..2902
                     /gene="mutL"
                     /locus_tag="VYO12_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1587402.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3227..3694)
                     /locus_tag="VYO12_000761"
     CDS             complement(3227..3694)
                     /locus_tag="VYO12_000761"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1587403.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(3695..4900)
                     /locus_tag="VYO12_000762"
     CDS             complement(3695..4900)
                     /locus_tag="VYO12_000762"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1587404.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(4920..5555)
                     /gene="ribE"
                     /locus_tag="VYO12_000763"
     CDS             complement(4920..5555)
                     /gene="ribE"
                     /locus_tag="VYO12_000763"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1587405.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5540..6640)
                     /gene="ribD"
                     /locus_tag="VYO12_000764"
     CDS             complement(5540..6640)
                     /gene="ribD"
                     /locus_tag="VYO12_000764"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1587406.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(6774..6888)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7045..7638
                     /gene="ruvA"
                     /locus_tag="VYO12_000765"
     CDS             7045..7638
                     /gene="ruvA"
                     /locus_tag="VYO12_000765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1587407.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            7648..8211
                     /locus_tag="VYO12_000766"
     CDS             7648..8211
                     /locus_tag="VYO12_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1587408.1"
                     /translation="MTKRCSWVKMTNPLYITYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8208..8885
                     /locus_tag="VYO12_000767"
     CDS             8208..8885
                     /locus_tag="VYO12_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1587409.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALIHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9022..10052)
                     /locus_tag="VYO12_000768"
                     /pseudo
     CDS             complement(9022..10052)
                     /locus_tag="VYO12_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
     gene            complement(10307..10990)
                     /locus_tag="VYO12_000769"
     CDS             complement(10307..10990)
                     /locus_tag="VYO12_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1587410.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11002..11946)
                     /locus_tag="VYO12_000770"
     CDS             complement(11002..11946)
                     /locus_tag="VYO12_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1587411.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12078..14909
                     /gene="uvrA"
                     /locus_tag="VYO12_000771"
     CDS             12078..14909
                     /gene="uvrA"
                     /locus_tag="VYO12_000771"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1587412.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILASVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            14902..15963
                     /locus_tag="VYO12_000772"
     CDS             14902..15963
                     /locus_tag="VYO12_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1587413.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASSLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16095..16493
                     /gene="spx"
                     /locus_tag="VYO12_000773"
     CDS             16095..16493
                     /gene="spx"
                     /locus_tag="VYO12_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1587414.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16559..17128
                     /locus_tag="VYO12_000774"
     CDS             16559..17128
                     /locus_tag="VYO12_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1587415.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGTATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17215..17481
                     /locus_tag="VYO12_000775"
     CDS             17215..17481
                     /locus_tag="VYO12_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1587416.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17485..17904
                     /gene="ruvX"
                     /locus_tag="VYO12_000776"
     CDS             17485..17904
                     /gene="ruvX"
                     /locus_tag="VYO12_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1587417.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            17920..18225
                     /locus_tag="VYO12_000777"
     CDS             17920..18225
                     /locus_tag="VYO12_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1587418.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18484..19734
                     /locus_tag="VYO12_000778"
     CDS             18484..19734
                     /locus_tag="VYO12_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1587419.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKDGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMREGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            19731..20273
                     /locus_tag="VYO12_000779"
     CDS             19731..20273
                     /locus_tag="VYO12_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1587420.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            20419..21951
                     /gene="cls"
                     /locus_tag="VYO12_000780"
     CDS             20419..21951
                     /gene="cls"
                     /locus_tag="VYO12_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1587421.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIMEIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22209..23747
                     /locus_tag="VYO12_000781"
     CDS             22209..23747
                     /locus_tag="VYO12_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1587422.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     YFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILY
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            23867..26074
                     /gene="nrdD"
                     /locus_tag="VYO12_000782"
     CDS             23867..26074
                     /gene="nrdD"
                     /locus_tag="VYO12_000782"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1587423.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTD"
     gene            26086..26226
                     /locus_tag="VYO12_000783"
     CDS             26086..26226
                     /locus_tag="VYO12_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587424.1"
                     /translation="MGKYQLDDKGCAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26270..26776
                     /locus_tag="VYO12_000784"
     CDS             26270..26776
                     /locus_tag="VYO12_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1587425.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            26769..27359
                     /gene="nrdG"
                     /locus_tag="VYO12_000785"
     CDS             26769..27359
                     /gene="nrdG"
                     /locus_tag="VYO12_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1587426.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27367..>27522
                     /locus_tag="VYO12_000786"
     CDS             27367..>27522
                     /locus_tag="VYO12_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1587427.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNPNY"
BASE COUNT         8111 a         5259 c         6005 g         8147 t
ORIGIN      
        1 agtatatatt agaaatgttt atgttcattc aaaagcataa tgactataac tgaaaaagaa
       61 aagtgatatc tttaaggttg ttcttcttgg tggtgagatt cgtgagacaa cccaagcttt
      121 tgtcggaaag attaccaatg ctttgatgga taggatgtac tttagcaaga tgtttttagt
      181 ggtaacggta tcgtggatgg acgtgtaata acctcttctt tcgaggagta ttttactaaa
      241 aaactagcct tggagcgttc cccagaaacg gacttactca ttgactcttc aaagatttgg
      301 ggagaagatt ttgcttcatc tgttccttga aaaaagtcac agcagtcatc acagacgata
      361 gtactgaaca aaactatgaa gagttagaaa tttatacgca ggtgattgta taaaggatct
      421 ggaaatagat aagaagttga ttagtattga cctaggtggt acaaatatta agattactgt
      481 tctttcaaat gacggtgaga ttgaaacttt gtggagtatt acaacagata caagtgagaa
      541 aggttctcaa attatatcgg acatcatcag ttctattaaa aataaattga ccgaacggaa
      601 tattcctgat agcgaccttc ttggaatcgg tatgggaagt tgctcatcat actttccttg
      661 taaatcatag gggctataaa ctctccgtct acttgtcctg caacaattga agtctgctca
      721 aaacgccgtc cgctaatctt ttcatagact ttctcccttt taggagccta gctttctagt
      781 ttgttctttg atttttattg agtataccac tattttactc cctctggcaa gggactttgt
      841 ctatgtggag ggattgggct cctatgtggt ggagcttttc tgttctttct gaaatatggt
      901 ataatagcac taatcaattt ctaggaaaat agatacagaa aggggctgaa agatgtctca
      961 tattattgaa ttgccagaga tgctggcaaa ccaaatcgcg gctggagagg tcattgaacg
     1021 tcctgccagt gtggtcaaag agttggtaga aaatgccatt gacgcgggct ctagtcagat
     1081 tatcattgag attgaggaag ctggtctcaa gaaggttcaa atcacggata acggtcatgg
     1141 aattgcccac gatgaggtgg agttggccct gcgtcgccat gcgaccagta agataaaaaa
     1201 tcaagcagat ctctttcgga ttcggacgct tggttttcgt ggtgaagcct tgccttctat
     1261 tgcgtctgtt agtgtcttga ctctgttaac ggcggtggat ggtgctagtc atggaaccaa
     1321 gttagtcgcg cgtgggggtg aagttgagga agtcatccca gcgactagtc ctgtgggaac
     1381 caaggtttgt gtggaggatc tctttttcaa cacgcctgcc cgtctcaagt atatgaagag
     1441 ccagcaagcg gagttgtctc atatcattga tattgtcaac cgtctgggct tggcccatcc
     1501 tgagatttct tttagcttga ttagtgatgg caaggaaatg acgcggacag cagggactgg
     1561 tcaattgcgc caagcaatcg cagggattta cggtttggtc agtgccaaga agatgattga
     1621 aattgagaac tctgacctag atttcgaaat ttcaggtttt gtgtccttgc ctgagttgac
     1681 tcgggctaac cgcaattata tcagcctctt catcaatggc cgttatatta agaacttcct
     1741 gctcaatcgt gctattttgg atggttttgg aagcaagctt atggttggac gttttccact
     1801 ggctgtcatt cacatccata tcgaccctta tctagcggat gtcaatgtgc atccaactaa
     1861 gcaagaggtg cggatttcca aggaaaaaga actgatgact ctggtttcag aagctattgc
     1921 aaatagtctc aaggaacaaa ccttgattcc agatgccttg gaaaatcttg ccaaatcgac
     1981 cgtgcgcaat cgtgagaagg tggagcaaac tattctccca ctcaaagaaa atacgctcta
     2041 ctatgagaaa actgagccgt caagacctag tcaaactgaa gtagctgatt atcaggtaga
     2101 attgactgat gaagggcagg atttgaccct gtttgccaag gaaaccttgg accgattgac
     2161 caagccagca aaactgcatt ttgcagagag aaagcctgct aactacgacc agctagacca
     2221 tccagagtta gatcttgcta gcatcgataa ggcttatgac aaactggagc gagaagaagc
     2281 atccagcttc ccagagttgg agtttttcgg acaaatgcac gggacttatc tctttgccca
     2341 agggcgagat ggactttaca tcatagatca gcacgctgct caggaacggg tcaagtacga
     2401 ggagtaccgt gaaagcattg gcaatgttga ccagagccag cagcaactcc tagtgcccta
     2461 tatctttgaa tttcctgcgg atgatgccct gcgtctcaag gaaagaatgc ctctcttaga
     2521 ggaagtgggc gtctttctag cagagtacgg agaaaatcaa tttattctac gtgaacatcc
     2581 tatttggatg gcagaagaag agattgaatc aggcatctat gagatgtgcg acatgctcct
     2641 tttgaccaag gaagtttcta tcaagaaata ccgagcagag ctggctatca tgatgtcttg
     2701 caagcgatct atcaaggcca atcatcgtat tgatgatcat tcagctagac aactcctcta
     2761 tcagctttct caatgtgaca atccctataa ctgtcctcac ggacgtcctg ttttggtgca
     2821 ttttaccaag tcggatatgg aaaagatgtt ccgacgtatt caggaaaatc acaccagtct
     2881 ccgtgagttg gggaaatatt aaaagtataa aaaagtctgg gaaaaatttt caaaatcaga
     2941 aaaacgcata aaatcaggtg ttcaaaaacc ttgattttat gcgttttatc atagaaatat
     3001 ttacttcatt ttttcctaat tcttttcgaa actcttttta aaccacgtca gttttatcag
     3061 taatctcaaa acagtgtttt gagctaattt tgccagtttt gtctgtaaca tcgaagttgt
     3121 gttttaccac tctgcgactg gtttcctagt ttgctctatg attttcacag agtattaaat
     3181 tgcgattttg ccaagtttct ttattcgtct aaaagtagag tctgttctat gcgtctaatg
     3241 tacgaattag gttgaccatt tcaatagctc cttgtgcaca ctcagaaccc ttatttcctg
     3301 ctttagtacc agctcgttct atggcttgtt caattgtatc tgtcgttaac acaccaaaca
     3361 taacaggaat ttcgctattt aaactgattt gggcgattcc cttagatacc tcgctacata
     3421 cataatcata atgacttgta ttccctctaa tgacagctcc caagcagata attgcatcat
     3481 attttttact ttttgccatt tttgatgcaa tcagtggtat ttcaaaagct cctggaaccc
     3541 aggctacctc gatatctttc tcgtttacat tctctctttt gagattatct agtgctccag
     3601 ataataattt tgaagttata aattcattaa atctcgctac aacaatacct attttaatat
     3661 tgtttgctac taaattacct tcataagtgt tcatttattt ttcctccata tttaaaatgt
     3721 gacccattcg atttttcttt gtttctaaat aaaaactatc gtaaggattg gcttctattt
     3781 cgattgatat tctactggaa atggtaattc catatttttc taactgttca accttgtcag
     3841 gattatttgt cagtaaatga agtgactgaa gtcccagatc tttaagcatt tgtgctccaa
     3901 tatgatattc tcttaaatca ccttcaaagc ctaatgcaag attggcatca agcgtatcca
     3961 tgccttgatc ttgtaaatga taggctttta atttattgat aagtccaatt cctcgtccct
     4021 cctgtcgcaa gtaaagtaag acacccgaac cattctcaac aatcattttc atagctttat
     4081 cgaattgctg tccacaatcg caacgtaaag agcctaaaac atctcctgtt aaacattcgg
     4141 agtggacccg acataataca ttggcttcat cctctatgtt tcccataata agagcaagat
     4201 gatgttcccc atttagttta tctatatagc taattgcttt gaaattaccg tatctagtag
     4261 gcatattgac agttgaaact cgttctacca gctgatcata tacttttcta tattcttgta
     4321 attctttgat ggtaattagt ggaatgttgt gtttcttcga gaactgaatt aaatcatctg
     4381 ttctcatcat tttgccatca tgattcatta tttcacaaca taggccacac tcttttagtc
     4441 cagctaattt taataaatca acagttgctt ctgtgtgtcc atttctttct agaacaccac
     4501 ctttttttgc aattaaagga aacatgtgtc ctggcctgcg aaaatcagag ggtgttatat
     4561 cttcagctac acacatacgt gcggtcagtc ctctttcctc ggcagaaata cctgtggtcg
     4621 tttctttata atcaattgaa actgtaaaag cagtcttatg attatctgta ttgttttcaa
     4681 ccataggtga aagcattaat tgattagcta aactttcgct cataggcata caaattaatc
     4741 ctttggcata agtagccata aaattaacat tttctgttgt agctgcttgt gcagaacaaa
     4801 ttaagtctcc ttcattttct ctatccttgt cgtctataac aagaacaagt cgtcccttct
     4861 gcaatgcttc taatgcttct tgtatttttc gatattccat tgactgatta tcctttctgc
     4921 taaaatccat tttgatataa tagttcctta gatatttctg attttggaga gttatccatc
     4981 agtttttgca catatttacc taagatatca ttttcaagat ttactgtact cccgacttgt
     5041 ttactcttaa gaatggtttg ttccaaggta tgagggataa cagatactga aaagtttact
     5101 ttggagactt tagcgacagt cagactaatg ccgtcaattg taatagatcc tttttcaact
     5161 attaaatcta aaatttcttt ttgtgtgttg atttgatacc atacagcatt atcatctttt
     5221 tttattgacg agatttttcc tgtaccatca atgtgtcctg taacgacgtg acccccaagt
     5281 cgaccgttga cagataaggc tctttctaga ttcacctcac ttccatgttt taatagagta
     5341 agagctgttc gactccatgt ttcattcatt acatcaactg taaaggattg atgattgaaa
     5401 tgagtaactg taagacagat accatttact gctatactat cgcctaaatg gatatccgtt
     5461 aatatttttg aggctttaat tgatagttta caattacgag agtctttctg tattctttca
     5521 acttttccga tttcttcaat tattcctgtg aacatggata aatcacttca ctttctatga
     5581 gatagtcatt tcctatttga gaaaaagcat aaggtttcaa tctaatagcg tcatttggca
     5641 aagaaatgcc ttcacctccg acaggaaact tggcactgcc tccaaaaatt tttggtgcaa
     5701 tatatatttt cagctcatca acaatttgtt gttccaaagc actccaattc attagactgc
     5761 ccccttctag aactaggcta tcaatctgca tgtttcctag atgttgcatt aaactcgata
     5821 agtctatatg attgcctttt ttctttatgg aaagtatttc acagccatga ttttgatata
     5881 gcttcatttt atttttgtct tcagaggaag tggcaatgta agttttaata tcatttgctg
     5941 tttttacgat tttagaggta agaggagttc gtaaatgtgt atcgcatatg atacggatag
     6001 gatttttccc ttcctccaat ctacatgtca gcaaaggatc gtcttgaata acagtattga
     6061 ctcccaccat aattgcacta acatagtgtc gtaactgatg cacatgcttt cttgcttctt
     6121 cttcagtaat ccatttggat tgatttgttt tagtggctat ttttccatcc attgacattg
     6181 catatttcat aaaaacatag ggtacatgct gggtaatata ctttctaaaa ctttttatta
     6241 agttaagaca ctcattttct aaaattccaa cagtaacttg aagattattt tcctcaagta
     6301 tctttactcc ttttccagat acaataggat tacagtctag gcttccaatg actactcttg
     6361 taataccact atcgattata gcatctatac agggaggtgt tttcccgaag tgacaacagg
     6421 gttcaagtgt tacataaagc gtcgctccga caggggattt tctacagttt ttaagagcat
     6481 ttctctcagc atgtgggcca ccaaaaaact catgataacc ttgtccgata atgtgattat
     6541 cttttacaat aactgcgccg accataggat tgggattgac gtaaccagcc cctttttgtg
     6601 ccagttttat tgctaatttc atatattttg aatcgctcat ctcactacct ccaaaaaaat
     6661 ataccttgaa taggggacta ctcaaggcat acaaaagaaa acttatgcga ttaacaaaaa
     6721 tgctctgaaa tgacaagtaa tcatttcaga gcacgcaaaa agcacaaata tacttttatc
     6781 ttctttcatc cagactatac tgtcggcttt ggaatttcac caaatcatgc ctttcggctc
     6841 gtgggctata ccaccggtag ggaattgcac cctgccctga agatagttat tcaattacag
     6901 atgattatag tacttaattt tgaatatgtc aacagataaa taccgattgt ttttgatata
     6961 ctgtatttgt gataatcgat tctcgctcct cggataaaga aaatatgata tactagataa
     7021 acgaaataag agagaaggaa cactatgtac gcatatttaa aaggaatcat taccaaaatt
     7081 actgccaaat acattgttct tgaaaccaat ggtattggtt atatcctgca tgtggccaat
     7141 ccttatgcct attcaggtca ggttaatcag gaggctcaga tttatgtgca tcaggttgtg
     7201 cgtgaggacg cccatttgct ttatggattt cgctcagagg atgagaaaaa gctctttctt
     7261 agtctgattt cggtctctgg gattggtcct gtatcagctc ttgctattat cgctgctgat
     7321 gacaatgctg gcttggttca agccattgaa accaagaaca tcacctactt gaccaagttc
     7381 cctaaaattg gcaagaaaac agcccagcag atggtgctgg acttggaagg caaggtagta
     7441 gttgcaggag atgaccttcc tgccaaggtc gcagtgcaag caagtgctga aaaccaagaa
     7501 ttggaagaag ctatggaagc catgttggct ctgggctaca aggcaacaga gctcaagaaa
     7561 atcaagaaat tctttgaagg aacgacagat acagctgaga actatatcaa gtcggccctt
     7621 aaaatgttgg tcaaatagga gcagagaatg acaaaacgtt gttcgtgggt caagatgacc
     7681 aacccgctct acatcaccta tcatgatgag gagtggggcc agcccctcca tgatgaccaa
     7741 gcattgtttg agttgttgtg tatggaaacc tatcaggcag gcctgtcttg ggaaacggta
     7801 ctcaacaaac gccaagcttt ccgagaagcc tttcatagct atcaaattca ctcagtcgca
     7861 gagatgactg acactgaatt ggaagccatg ctggagaatc cagctatcat tcgaaataga
     7921 gccaagattt ttgctacacg cgctaacgcc caagcctttc tacagttaca ggcagagtac
     7981 ggctcttttg atgcctatct ttggtctttt gttgagggga aaactgtcgt taacgatgtt
     8041 cctgattatc gccaagcccc agctaaaaca cccttatctg agaaattagc caaagatctc
     8101 aaaaaacgag gcttcaagtt cacaggccca gtcgccgtat tgtcttttct acaggctgca
     8161 gggctagttg atgaccacga gaatgattgt gagtggaaag gtcttaaatg atgtctaaca
     8221 aaaataaggg aattctgatt tttgcgattc tctatacagt cctctttgtg tttgatggcg
     8281 ttaaattgct ggcttcttta atgccatctg ccattgcaaa ttatcttgtt tatgtagttt
     8341 tagctttata tggctccttc ttgttcaagg atagattgat ccaacaatgg aaggagatta
     8401 gaaagactaa aagaaaattc ttctttggag tcttaacagg atggctcttt ctcattctga
     8461 tgactgttgt ctttgaattt gtatcagaga tgttgaagca gtttgtggga ctagatggac
     8521 aaggtctaaa tcagtctaat attcaaagta cctttcaaga acaaccacta ctgatagctg
     8581 tttttgcttg tgtcattgga cctctggtag aagaattatt tttccgtcag gtcttattgc
     8641 attacttgca ggaacggttg tcaggtttac taagcattat tctggtagga cttgtttttg
     8701 ctctgattca tatgcacagt ttggctctat cagagtggat tggtgcagtt ggttacttag
     8761 gtggaggcct tgccttttct attatttatg tgaaagaaaa agagaatatc tactatcccc
     8821 tacttgttca catgttaagc aacagcctct ccttaatcat tttagctatc agtatagtaa
     8881 aatgaaatga gaacaggaca aatcgatttc taacaatgtt ttagaagtag aggtgtacta
     8941 ttctagtttc aatatactgt aatatgtgat gaaaatgcca gtaatgatac cgagaaaaaa
     9001 gctgagaaac ttttcccagc tttatttgtt atagtcaaag agaatgactt gttcctgtgc
     9061 atctacatga gcatggaccc caaagggtac aattgctctt ggagttgcgt ggccgacatt
     9121 cagattatag acaatcggga tattgctgtc aatgatatcc aatagtgcct ctttatagtc
     9181 gtcatggaaa gtttcatcca taggttttcc gaccaagagt ccattgatga ccgcgaatat
     9241 gccagtgtcc tttaaagtta gcaacatctt tttgaagtct tctggcttag gcttttcttc
     9301 gcttgtttcg agcaagagga ttttcccttc ccagtctgac aagtcaggga aaagtttgta
     9361 tttttggcag agttccgtgc tatctgcgta tcgagagttg tcaaagatat cgtagaggga
     9421 ttcgaggcaa ccaccgagga ttttcccctc gaactgggca cttccttgca acaagtcaaa
     9481 acctgtattt gtatgactga cacgaggtgt tcccagggcc gtgggactaa aatcagttcg
     9541 ttcctcatac caaacgtcac tagggcggat ttctgaaatt cttcccgtct caatcaattc
     9601 tttaaagtag tgaaggctat aggctagcat ttctttgtct aattcacaaa tgtctgctaa
     9661 aaaggattga ccataaaaag tcttgattcc taatttatgc aacatgaggt ggttcatggt
     9721 tgtatccgag aagccaagaa aaattttttg tttgataccc ttttggagtt ggtcattttc
     9781 aaaaagataa ggtagcaagc gataggcatc gtctccaccg atggcacata ggatcatgtc
     9841 gatctatcat cagaaaaggc atgaatcaaa tcctctgcac gagcttcagg atggtccttg
     9901 ataaagtcta atccttttaa cgaatggggc aaaaagatgg gattgagtcc cagatccttg
     9961 agacgttgga tacccaagtc cacttcgtgt ttgacaaaat cctctccgat aatgccacta
    10021 gataaactaa caataccaat agtagaaacc atatctcatc ctcctagaaa tagattgagc
    10081 ctattttatc acaaaatata agagaaagct atgtgggatt tcagaagaat acttttaata
    10141 ctcttcgaaa atctcttcaa accacgtcag ttttatttgc aacctcaaag ctgtgctttg
    10201 agcaacctgc agctagcttc ctagtttgct ctttgatttt cattgagtat aactcaaaaa
    10261 agtaagtgaa aaagcaaccg cacctgcagt tgcttttatc gttttcttat tcttgatagc
    10321 gtgttggtcc tgcacttgca ctacggatat tcaagcgttt cttgagatag aagcgaaggg
    10381 ctaggagggc tgctccaata atagctagtg gcaatggatc cagtactggg ttaaggctag
    10441 ctggtaggaa gcttgttgca aagaagacaa gcaaccaaag gaacatagaa gctaggataa
    10501 ctagtacaga tttccagaaa ggtggacgtt gactgcgatc catatctggt ccatagtatt
    10561 ggtaaacaaa gtagtacatc aagtagaagg caaatccacc aaccagtcca actaatagaa
    10621 gagtaatcaa tccatagccg aaagcttgat ctgccgcaaa gaaggttgtg agggcgctga
    10681 caagggcaaa gaggctagtg atgaaaagag ctgagtccat aatcatcagt tttggatcat
    10741 cattttcttt tggatgctct ttttcgtact gctctttgac agtgaagcta tgagcccaat
    10801 gagttggtgc gccgtatagg gaacgggcag ttgtaccttt agattgctcc tcaaggattt
    10861 ggggaatgac ttcctcaaaa atagtctgga tttcagcgtc tgttttccca tctttgatga
    10921 attgttgggt agcaatgtgg acaaactctt ggtttttctt agttaatttt tgtagatcaa
    10981 tttgagacat aggaactcct cttagaacca ttttttatgg atgagataga gagtgagcga
    11041 gacactcata gcaaaggcga taaagacgat taaccagaag gcatttggct ctccgtttag
    11101 ggggatttca ttatccttaa agttcatccc gtaggcagaa aagaccatgg ttgggatgga
    11161 catgacgatg gtcacaaggg ccaaggtttt catgatgttg ttctggttgt tagaaatgat
    11221 agaggcaaag gtctctgtca tagaatgcaa gacgtttcca taaatatctg ccatctcgat
    11281 ggcctgttgg gtttcaatca gggtgtcttc aagcaggtct tcgtcttcaa ggtatttctt
    11341 gatattgctg gttgaactgg tcaatttctt aatcacgcgc tcatttgttt tgagggaggc
    11401 cttgaaatag acgatagttt tttccaattc catgagctca atcaattctt cattacgagt
    11461 tgattgatgc agttgacttt cgatttgttc actcttgcgg tcgattgaac gaagggctgt
    11521 taggtaaagc tctgcattgc gataaagaat ttgaaagata aaacgtgaac gcatgaaggt
    11581 atagaaatta cgcaatcgac ggttgataaa gacatcaagg acaggtagtg gttccaaaca
    11641 cgtagtgata atggtttcct cagtgatgat aataccaagc gggatggtta cgtagtaggt
    11701 gcggttattt ctttcctccg tgaccggcac gtctacgata atcagggtat actcgtcttc
    11761 aatggtaata cgagacattt cttccgcatc gagcggtgct cgaaggtcgg caatatcaat
    11821 atcgaaggtg ttggcgattt cgagtgattc attttgagtc ggattgacga gattgatcca
    11881 agtacccggt tcaagcgtat cgatctcttt aaattcagtt gttgtagaga gaaaaacttg
    11941 tttcatatcc cttatccttt ctcatttttc agattttttc acactgtact attatactac
    12001 aaaatcggcc ttttgggtaa tagaatctca ctttttttgg tataatggta agcaataatg
    12061 gactagaaag aacaaagatg caagataaaa ttgtcattca tggggcgcgt gcccataatt
    12121 taaaaaatat tgatgtggag attccgcgag acaagttggt tgtcgtgact ggcttgtcag
    12181 gttcagggaa gtccagtctg gcttttgata ccctctatgc ggagggacaa cgtcgctatg
    12241 tagagagttt gtccgcctac gctcgtcagt tcttgggaaa tatggaaaaa ccagatgtag
    12301 atgccattga tggtctcagt ccagctattt ctatcgacca gaaaacgact agcaaaaacc
    12361 ctcgctcgac ggtgggaaca acgactgaaa tcaatgacta tctgcgtctc ctctacgcac
    12421 gtgtggggac gccttactgt atcaacggac atggagctat caatgcttct tctgtggagc
    12481 aaatcgttga taaggttttg gagttacctg aacgccagcg cttgcagatc ttggcctctg
    12541 tcatccgcaa gaaaaaaggc caacataaga gtgttatcga gaaggttcag aaagacgggt
    12601 atgttcgtgt ccgtgtggat ggggaagtct atgatgtgac cgaagtgcca gagttgtcta
    12661 agagcaagca acacaatatc gatgtcgtgg ttgaccgtat tgttatcaag gagggcattc
    12721 gtagtcgtct ctttgattcc attgaggctg cccttcgtat tgcagaaggt tatgtcatta
    12781 tcgacaccat ggacgactct gagttgttgt tctctgagca ttatgcctgt ccagtttgtg
    12841 gttttactgt cccagagtta gagcctcgtc tcttctcttt caatgctcct tttggctctt
    12901 gtagtgagtg tgacggcttg ggcatcaagc tggaggtgga tactgatttg gtagtgccag
    12961 atgccagcaa aaccttacgt gagggagcgt tagcaccttg gaatcctatc tcatccaact
    13021 actatccaaa catgttagag caggccatga aagtatttgg agtggctatg gataagcctt
    13081 ttgaggacct gtcagaagaa gataagaact tgattctcta tgggtcagat ggtaaggaat
    13141 tccatttcca ctatgagaat gaatttggtg gcgtgcgcga tatcgacatt ccgtttgagg
    13201 gagttatcaa taatatcaaa cgtcgttacc atgaaaccaa tagcgattac actcgcactc
    13261 agatgcgtct ctacatgaat gagctgacct gcggaacctg tcaaggctat cgtctcaatg
    13321 accaggcctt gtctgtccgt gtgggcggcc agcaagggcc acatattgga gaaatctcag
    13381 acctgtctat cgctgaccac ttggacttgg tgagccagtt aactttgtct gaaaatgaag
    13441 ccatcattgc tcgtcccatt ctcaaggaaa tcaaggatcg tttgaccttc cttaataatg
    13501 tgggtcttaa ctatctaacc ctgtcacgtt cagcaggaac cctttcaggt ggggaaagtc
    13561 agcgcattcg tttggcaacc cagattggtt ccaacctatc aggcgtcctc tatatcctag
    13621 acgagccgtc aatcggtctt caccagagag acaatgaccg cctgattgcc agtctgaaaa
    13681 agatgcgtga cttgggcaat actcttatcg tggtggaaca cgacgaagat accatgcgtg
    13741 aagctgatta tctgattgac gttggtcctg gtgccggtgt ttttggtggt gagattgttg
    13801 cagcaggtac acctaagcaa gtggctcgca acagcaagtc tatcacaggt cagtacttgt
    13861 caggcaaacg tgtcatccca gtaccagaag agcgccgtgt cggaaatggt cgttttattg
    13921 aagtgatagg agcgcgtgag aacaacttgc aaaatgtcac ggctcgcttt ccactaggaa
    13981 aatttatcgc agtgacaggt gtgtcgggtt cagggaaatc gactctaatc aacagcattc
    14041 tcaaaaaagc cattgcccag aagctcaacc gtaattcaga caaacctggt aagtttaaga
    14101 ctattacagg gattgagcat gtcgaccgtt tgattgatat tgaccagagc cctatcggac
    14161 ggacgccgag gtctaaccca gctacctata caggagtttt tgacgatata cgtgaccttt
    14221 ttgctcagac aaatgaagcc aagattcgag gctacaagaa ggggcgcttc agtttcaacg
    14281 tcaagggagg tcgctgtgaa gcttgctcag gtgacgggat tatcaagatt gagatgcact
    14341 tcttgccaga tgtttatgtg gcttgtgaag tttgccacgg aacccgctat aacagtgaaa
    14401 ccctagaagt tcactacaag gaaaagaata tttcgcaggt cttggatatg acggtcaacg
    14461 atgcggtaga atttttccaa cacattccga aaattcaacg caaactccag accatcaagg
    14521 atgtagggct aggctatgtg accttgggac agccagctac caccctttct gggggagaag
    14581 cccaacgtat gaagttagct agcgaactcc acaaacgctc gacaggaaaa tctttctaca
    14641 ttctggatga gccgacgaca gggcttcata cagaggacat tgctcgcctg ctcaaggttt
    14701 tagctcgctt tgtagacgat ggcaatacag tcctcgtcat cgagcacaat ctggatgtta
    14761 tcaagacggc agaccatatc attgacttgg gacctgaggg cggtgtcggt ggtggaacca
    14821 tcatcgtaac aggaactcca gaagaagtag ctgccaatga agccagctat acaggacact
    14881 atttgaaagg aaagttacat catgaataaa cgcgtacaag catttctagc taaaatgcaa
    14941 gaaaaagaac tagatggtat catcatcaac aatcttaaaa acgtctatta tttgactggt
    15001 ttttggggct caaacggaac agtctttatc agccgtgacc gtcaggtctt agtgacagac
    15061 tctcgctata tcatcgcagc taagcaagaa accagtggtt ttgagattgt ggctgatcgt
    15121 gatgaattgg ctgtcattgc aggaattgtt aaggacatgg gcttgactcg tatcggtttt
    15181 gaagatgaga tttcagtatc ttattaccac cgtatgcagg cagcttttgc aggtttggac
    15241 ttgcttccac aaactcaatt tgtggaaggt cttcgtatga ttaaggatga ggcagagatt
    15301 gcagcgattc gcaaggcttg ttctatctca gaccaagctt tccgcgatgc gcttgacttt
    15361 atcaaaccag gaaaaactga aattgaaatt gccaacttcc ttgatttccg catgcgtgag
    15421 ttgggagcat ctagcttatc ttttgatacg atcctagcta gcggtatcaa ttcttctaaa
    15481 ccccatgccc atccaatgca caaaccagtg gagttgggag aagccattac catggacttc
    15541 ggctgtctct atgaccacta tgtcagtgat atgacccgga ctatctatct agggcatgtt
    15601 agcgatgagc aggcagagat ttacaatacg gttctaaaag ctaaccaagc cttgattgac
    15661 caagctaagg caggcttagg tttccgtgac tttgacaaaa tccctcgtga tattatcatt
    15721 gaggcaggtt atggcgacta ctttactcac ggcattggcc acggtattgg tctggatatc
    15781 catgaggaac cctactttag ccagacttct acagaaacta ttaagacagg tatggccttg
    15841 accgatgaac caggtatcta tatcgaaggc aaatatggcg ttcgtatcga ggatgatatc
    15901 ctgattacag agacaggttg tgaattattg accctagctc caaaagagtt gatagtcatt
    15961 tagttgtttg tcaagaaaaa agtacgaaaa tgacgaaaaa agtcaaaaaa aattaaaaat
    16021 aggtcgcaag tcggatgttt tttatggtat aatagactaa actgatagta acatgtagcg
    16081 aaaggggtag gtacatgatt aaaatttata cagtctcaag ttgtactagc tgtaaaaaag
    16141 caaaaacctg gctcaatgcc caccagttaa gttataaaga acaaaacctt ggtaaagaag
    16201 gaattacgag agaagaatta ctggatattc taaccaaaac agataacgga atagccagca
    16261 tcgtttcgtc taaaaatcgc tatgccaaag cccttggagt ggatattgaa gatttgagtg
    16321 tcaatgaagt tctcaatctg attatggaaa caccgagaat tttaaagagc ccaatccttg
    16381 tagatgaaaa acgcctgcaa gttggctaca aggaagacga tattcgtgcc ttcctaccac
    16441 gctctgtccg taatgtagaa aatgcagaag cacgtttacg tgcagctcta taaacatcaa
    16501 ggctgggagc acttcccagt cttattctat tttaatttca aaaagaaaga agagagaaat
    16561 gaaaaaaata gttcttgtta gtctagcttt cctttttgtc ctggttggtt gcggacagaa
    16621 aaaagaaact ggaacagcta caaaaacaga aaaggatacg cttcagtcgg cattgccagt
    16681 tattgaaaat gccgagaaga atacagttgt aaccaagact ttggtcttgc ccaagtcaga
    16741 tgatggtagc cagcaaactc aaaccattac atacaaggac aagacttttt tgagcttaac
    16801 gattcaacaa aaacgtccag tttctgatga gttgaagact tatatcgacc aacatggagt
    16861 ggaagaaact caaaaagctc ttcttgaagc ggaggagaag gataagtcta tcattgaagc
    16921 tcgtaaattg gcaggtttca aacttgaaac aaaactattg agcgcaacgg aacttcaaac
    16981 aacgactagt tttgattttc aagttctgga tgtcaagaag gcttcccagt tggaacatct
    17041 gaagaatatt ggtttggaaa atcttttgaa aaatgaacca agcaaatata tttcagatag
    17101 attggcaaat ggcgcgacag aacaatagaa aatccaaata aatagactgc ctgaattgta
    17161 gaaggtaagg aattatgcta taatggtact attctaagga aagagggtgt tagaatggga
    17221 tttactgaag aaacagtacg ttttaaattg gacgattcca ataaaaaaga aattagcgaa
    17281 actttgacag atgtttatgc ttcgttgaac gataagggtt acaacccaat taaccaaatc
    17341 gtaggttacg tattgagtgg agaccctgcc tacgttcctc gttataataa tgcacgaaat
    17401 caaatccgta agtatgagcg tgatgaaatc gttgaggaat tggttcgtta ctacctcaaa
    17461 ggacaaggag tcgatctata acctatgaga attatgggat tggacgtcgg ttcaaaaacg
    17521 gtaggggtgg cgattagcga tccgcttggt tttacagctc aagggcttga aatcatccag
    17581 ataaatgaag aacaaggcca atttggtttt gaccgcgtta aggaattggt tgatacttac
    17641 aaggtagaac gatttgtagt gggcttgcct aaaaacatga acaatacaag tggaccgcgc
    17701 gtagaagcta gtcaagccta cggagcaaag ctagaagagt tttttggttt accagtagac
    17761 tatcaggatg aacgcttgac aacagtggct gctgagcgca tgttgattga acaagcagat
    17821 atcagtcgca ataagcgcaa gaaagtcatt gataagttag cagctcagct gattttacaa
    17881 aattatttag atagaaaatt ttaatataaa ggagaggcta tgtcacacga tcataaccac
    17941 gaccacgaag aacgtgaact aatcacacta gtagatgagc aaggaaatga aaccttgttt
    18001 gaaatccttt tgacaattga tggaaaagaa gaatttggta aaaactatgt tcttctagta
    18061 ccagttaacg cagaagaaga cgaagacgga caagttgaaa tccaagctta ctcattcatt
    18121 gaaaatgaag atggaacaga aggcgaattg caaccaatcc cagaagactc agaagacgaa
    18181 tggaacatga ttgaagaagt cttcaacagc tttatggagg agtgatacag tctgggagac
    18241 tgtatcagcc caactcccag aaatggggaa gagttggaac gtccagtgaa catttttacc
    18301 ccaactctta gaacttgaga agagttggaa catctagtgg atgtttttag cccatattca
    18361 cattcatagt agctagttat tagctaacca ggtaagaggt cgggacgaaa atcctggcct
    18421 tgtttttatt agtaaccata ctttaatgcg ataattgatt ggacttttgt caaggagatg
    18481 tgtatgtttg aagtagaaga atggctccat agtcggattg gtttgaattt tcgatcaggt
    18541 ttgggccgaa tgcagcaagc ggtggatttg ttaggaaatc ctgagcagtc ttaccctatt
    18601 atccacgtaa cagggactaa tgggaaagga tctaccattg cttttatgag ggaattattt
    18661 atggggcatg gcaaaaaagt tgcgaccttt acctcccctc atatcgtctc tatcaatgac
    18721 cgaatctgca ttaatgggca acctatagca gacgcagact ttatccgttt gactgatcag
    18781 gtcaaagaga tggagaaaac gcttctgcaa actcctgccc agttgtcctt ttttgaattg
    18841 ctgaccttag ttgcttttct ttattttagg gagcaggagg tggatttggt tttattagaa
    18901 gtgggaattg gtggcttact tgacacgacc aatgtggtaa ctggagagct tgctgtcatc
    18961 acctccattg ggcttgacca tcaagaaacc ttgggtgata gtctagaagc aattgcagag
    19021 cagaaagctg gtattttcaa ggatggtaag aaggcagtga ttgcgaaatt gcctccagaa
    19081 gctaggcttg tctgtcagaa aaaagccgaa tctttagctg ttaaccttta tcaggcaggt
    19141 caagattttt taatgctgaa tggtgatttt tcaagctctt tactaaatct ttcacagctg
    19201 aacataggct tagaaggagt ctatcagcag gagaatgcag ccttggcgtt gcaaactttt
    19261 cttcttttta tgagagaagg aaaggaagct gttgatgaac aggctgtaag aaaggccttg
    19321 gaacagaccc attgggctgg tcgcttggag cgtattcgcc cacagattta cttggatggt
    19381 gctcataacc tccctgcctt gactcgcttg gttgagttta tcaaagaaaa agagcaggaa
    19441 ggctatcgac ctcaaatcct ctttggatcc ttgaaacgga aggattatca agggatgttg
    19501 ggttatctga ctgaaaattt acctcaagtg gaactcaaaa tgaccggctt tgactatcaa
    19561 gggtctttgg acgagacaga tgtcacaggt taccatgtaa ttccctctta ccgagaattt
    19621 atcagcagtt tcgaagaaag ggcagacgct caagacttgc tgttcgttac agggtctctc
    19681 tattttatct cagaagtacg gggctacctg ctggaccgtg agcagataaa ttgaccccct
    19741 tgtttgaact tgttatacta ggggaattgc tggttagaga aaatttctct aaattggtag
    19801 cagaaaggaa attcatcatg aaattaaaaa gattcacact ttctcttgct tctctagcaa
    19861 gttttagtct cttagtagct tgttcacaaa gagctcaaca ggttcaacag cctgttgctc
    19921 agcaggtcca acagcctgct caacagaata ccaatactgc aaatgcagga ggtaaccaaa
    19981 atcaagcggc tccagtacaa aaccaacctg ttgctcaacc gaccgatatt gatgggactt
    20041 atactggtca ggatgacgga gaccgtatca ctttagtggt aactggaacg actggtacat
    20101 ggactgagct cgaatctgac ggggatcaga aagtcaaaca ggttacattt gattcagcaa
    20161 atcaacgcat gattattggc gatgatgtca aaatttacac tgtaaacggt aatcaaatcg
    20221 tcgtagatga tatggataga gacccatcgg accaaatcgt tttaactaaa taagactatg
    20281 caagtagaaa gggctggatt tttcagcctt tttactttta cctagaaaat aatcataaat
    20341 acttgccttc taccgaatac ctgcgttata ctagtatcaa ttacttgttt ttagcattca
    20401 aactatcaag gaggggatat gaaatatagg aaatttcaat tattgatgtc caagtatggc
    20461 tttagtcttt cgattatgct acttgaactt tgtcttgttt ttggtctctt tctttattta
    20521 ggacgcatgg ctcccatttt atggattact gtcctcattc tactgagtat catcacaatc
    20581 atttcgatag tcaaccgtaa tacgactcct gagaataagg taacctggtt gttagtagcc
    20641 tttgtgccag tatttggtcc cttgctctat ctgatgtttg gtgaaaggcg attgtccaaa
    20701 aaagaaatca aacaactgaa gaagctaggc tctatgcatt tccaagaagc aaatagccag
    20761 ctactaaaag agaaattaaa agaaagtgac aaggcagctt atggagtcat caagtcctta
    20821 ttgagtatgg ataccaatgc tgacatctat gatcaaactg cctctacatt ttttcctaac
    20881 ggagaagcta tgtggaaaaa gatggtagaa gatcttaaaa aggctgagaa atttattttc
    20941 ttggaatatt acattataga agaaggtttg atgtggaatc gcatactaga tatactagag
    21001 caaaaggtag ctcagggtgt agaggttaag atgctctatg atgatatcgg ctgtatggct
    21061 actttaacag gagattatgc acatcgactt cgtcagctgg gcatcgaggc ccataaattc
    21121 aataaagtta ttcctcgttt gacagtggct tataataaca gagatcatag aaaaatattg
    21181 attgttgatg gtcagatagc ctatactggt ggggtcaatc tggcagatga gtacattaac
    21241 cacgtcgaga gatttggtta ttggaaggat agtggaattc gcttagacgg actagcagta
    21301 aaagccctga cacgcttatt tttgaccact tggtacatta atcgaggaga aattagtgat
    21361 tttgatcaat atcatttaga aaatcattct atcccgagtg acggtttaac cattccatac
    21421 ggaagtggac ccaagccaat ttttcgagcg caggtaggga aaaaagttta tcagagttta
    21481 atcaatcaag caacggaatc ggtctatatt acgacacctt atttgattat agattatgat
    21541 ttaacagaga caatcaaaaa tgcagctatg agaggggtcg atgttcgaat tatcacccct
    21601 tacataccag ataagaagtt cattcagtta gtcacgagag gggcttatcc cgaccttctt
    21661 tctgctggtg ttcggattta tgagtatagt ccaggtttta ttcatagtaa gcagatgttg
    21721 gtagacgaag attttgcggt ggtggggaca atcaatctcg actaccggag cttggtacac
    21781 cattatgaaa atgcagtctt actctataaa actccttcta taatggaaat cgcccgagat
    21841 tttcgaaata tatttgcaga ttctcaggaa gtctatcctc attctatcaa aacgagctgg
    21901 tatcaaaagc ttgtaaaaga aatcgcccag ctattcgccc ctatcttata agaaatctag
    21961 aacttaggat acaaaccaga cctatttttc ttgtctttta cgaatagaaa gaaagaggag
    22021 gggaattcat gctaactaag gaagaagtga atgatatgat tgagttcaaa cagacacatt
    22081 tacaccgctt tcgggaaatt gaaacctttg tgaaactatg caaaaaatca ctcaaacagc
    22141 catcgcaagc tgacaatcct aggacttttt gatatactaa gacagataaa ttgaacagga
    22201 gaaacgatat gagtgtgtat ggtagagtag aagaagttca taaggagaat cgtgaacctc
    22261 tagaatacca aatcgaacaa gaatcgcatc atcgtgaatc aagtcgtctt cctttggtga
    22321 aaattttact atggagtacg cttgtaacgg ggataactct aggagtaccg ctattactcg
    22381 acttaatgag tgcacaagaa gtgcaggatt tttatgcagg ttgggcgctt catcagacag
    22441 ggaagattta cagcgactat tatggaagtc aaggtttgct ttattatttg ctgacttacg
    22501 tgagtcaggg cggatatttc tttgccatct ttgagtggtt agccttggta gcaggaggat
    22561 ttttcctttt tagatcagcg gacaccttga cagagcaagg agaccaagct ggacatctgg
    22621 tgactatttt ttacatgcta gttacaggtc ttgcttttgg tggaggctat gcgactcttt
    22681 tagcgcttcc tttcttattc gcagccttta gtttagttgc ggcttaccta agcaatccaa
    22741 gccatgataa gggatttgta cggattgggc tagctttggc aggcggattt ttctttgctc
    22801 ccttatcatc gctcctgttt attgctgtag tgagtttagg cttgttggtc tttaaccttg
    22861 ggcatagacg ttttgtgcat gggttttatc agtttcttgc agtggcttta ggtttttcac
    22921 ttgtctttta tccaactgcc tactatagtg ctgcaacagg aagttttggg gatgcgatta
    22981 gtggtattcg ttatcctatt gacagtattc gctttgattt tacttctaaa attttagaga
    23041 atatgttttt ttatggcttg ttgtcccttg gtttgggatt tgtggtttgt atctttttag
    23101 gtctctttca atccaaaccc tttaaactat acgtcatttc ggttcctgca agtcttgtgg
    23161 taattttagg tttgatttta cttttctttt cacaagagcc tctgcacgct tcttatttga
    23221 tggtcgtctt ccctgttttc ctacttttat tggtaaccaa tattaagagt caacagaggg
    23281 ggcgtagtgc tagaagaagc cgaagagaaa cgccagttag cctatggagt cgtttcttca
    23341 aaggaaatct atatctgcta gtttttgggt ttgtctatct tttgtctgtt ccttttttga
    23401 tgaagtttgt cctttatcca gtaccttatc aagaacgtaa tcgtcttgct gatttggtaa
    23461 aagaggagac aaatacggaa gatgctatct atgcatggga tgatactgcg actctttatc
    23521 gtaagagtga gcgcttgtcg ccatcggcga ttttgtaccc gttgcactat acagcaactg
    23581 aagaaaatcg taataagtta cttaatgact tgaaagaaaa acaacctaag gtgattgtgg
    23641 taaatgacaa ggtggtagtc tggtctgaag tggaaacact cttaaaagaa aattaccaac
    23701 aagtaaagac tgattactca gagtttaaag tctataaaat taaataacca aatcaatatc
    23761 ttgtgtattt ttaaaaattt taggattttt aacacaagat attgattttt ctttttagag
    23821 tggtataata ctttttagaa agaacatttt agaaaagagc atgcatatga ttgcactaga
    23881 agaaaaaatt acaattttgc caactctctt cgtcgagaaa cgagatggga gacgtgttgt
    23941 atttgatgtg gacaagattg acaaggctct ccacaaggcg gctgacaagg ttatggatgt
    24001 gacacccctg gttgaaaaac gcctcaatga tctgactgag cgaattatta cagaaattca
    24061 tagtcgcttt ccacagggaa ttaagattta cgaaattcaa aatatcgtag aacatgaact
    24121 ccttgaagcc aaagaatatg cactggctga ggagtatatt acttatcgga cacagaggga
    24181 ttttgagcgc tcaaaagcga cggatatcaa ctttagtatt cataaacttc tcaacaaaga
    24241 ccagacagtt gtcaatgaaa atgctaataa agacagtgat gtctttaaca ctcagcgtga
    24301 tttgacagca gggattgttg ggaaatcaat cggactgcaa atgcttccta agcacgtagc
    24361 caatgcccac caaaaggggg atatccacta tcacgatttg gactacagtc cctatacccc
    24421 tatgaccaac tgctgtttga ttgattttaa gggtatgttg gaaaatggtt ttaagattgg
    24481 aaatgcagag gtagagagtc ccaagtctat ccagactgcg acagcacaga tttctcaaat
    24541 cattgccaac gttgcttcta gccagtacgg tggctgttca gctgaccgta tcgatgaaat
    24601 tttggcgcct tatgcagaga agaactatca aaaacatctc aaagatgcag aagagtgggt
    24661 attgcctgaa aaacgggaag attacgcttg gaagaaaacg caaaaggaca tctacgatgc
    24721 catgcaatct cttgagtatg aaatcaatac tctcttcact tcaaatggac aaacaccttt
    24781 tacttcgcta ggttttggtc tgggaaccag tcgttttgaa cgagaaattc aaaaagctat
    24841 tttaaacatt cgcatcaagg gtcttggttc agaacaccgt acggctatct ttcctaaact
    24901 tattttcact ctgaaaagag gccttaattt agaggaagga actcccaact acgacatcaa
    24961 gcagttggct cttgagtgtg caaccaaacg gatgtatcca gatgttttat cttacgataa
    25021 gattgttgac ttgacaggat ccttcaaggt gcctatgggt tgccgttctt tcctccaagg
    25081 atggaaggat gaaaatggtg tagaagtcaa ttcaggtcgc atgaatctgg gtgttgtgac
    25141 ggttaatctg cctcgtattg ctcttgagtc tgaaggtgat atgaataagt tctgggaaat
    25201 cttcaacgag cgaatgaata tcgcagaaga tgctcttgtt taccgtgtcg aacgcactaa
    25261 agaggcgaca ccagcgaatg ctcctatcct ttatcagtac ggtgcttttg gccatcgtct
    25321 aggtaaagaa gaaagtgttg accagctctt taagaatcgt cgtgcgaccg tttcgctggg
    25381 ctatatcggc ttgtatgaag tagcgacagt tttctttggt aacagctggg aaagtaatcc
    25441 agatgctaag gaattcacgc tagacatcat tcacgatatg aaacgccgtg tagaagagtg
    25501 gtcagaccaa tatggctacc atttctctat ctactcaaca ccatccgaaa gtctgacaga
    25561 ccgtttctgt cgactagata tagacaagtt tggctctatt cctgatatca cagacaagga
    25621 atactacacc aactctttcc actacgatgt tcgtaaaaat ccaacaccgt ttgaaaaatt
    25681 ggactttgag aaagtctatc cagaagcagg tgcgtcaggt ggtttcatcc attattgtga
    25741 gtatccagtc cttcagcaaa atccaaaggc cttggaagct gtctgggatt atgcttatga
    25801 ccgtgtaggc tatctaggca ccaatactcc gattgaccgt tgctacaagt gtgactttga
    25861 aggggatttt gaaccaactg agagagggtt tgcttgtcca aactgtggca atagcgaccc
    25921 taaaacagta gatgtggtga agcgaacttg tggctaccta ggtaatcctc aagcaagacc
    25981 gatggtcaac gggcgtcaca aggaaatcgc tgcgcgtgtc aaacatatga atggttcaac
    26041 gattaaaata gctgggcatc aagtaacaga ttagaaagaa atgaaatggg aaaatatcaa
    26101 ctagacgata aggggtgcgc acaagtgacc cgttatcacg agaaacactc taaaggtgga
    26161 gctggtaaga aagaacgctt gcttagcttc agagaacaat ttttaaacaa gaacaagaaa
    26221 aaataaaagt gagagccagc tctcgctttt ctcatagtgg gaggtaagga tggaattacg
    26281 cagaccaaga ttagcggata agaaagctgt tttagatatg atgacagagt ttgaaaaatt
    26341 tcagtcgcct cacgacggcg gtttctggga tacagagaac tttgtgtatg aagactggtt
    26401 agaaagcaat caggaacagg aaatggggat taatctgcct gaaggatggg ttcctgcaat
    26461 tcagttagtg gctttttctg agaaaggtca agcagttgga tttcttaatc tccggttgcg
    26521 cctcagtaac tttctactag aagaaggtgg ccacattggc tactccattc gtccatctga
    26581 aagaggcaag ggttatgcaa aagagactct ccgtcagggc ttgcaagttg ctaaggaaaa
    26641 gaacatcaag aaagctctgg tgacctgtag cgtgaataat cctgctagca gagcagtcat
    26701 tctagcaaat ggtggaatat ttgaggatgc tcgcaatgga gtcgagcgtt attggataga
    26761 ggtagcgaat gaataatcca aaaccacaag aatggaaaag cgaggaactt agtcaaggtc
    26821 gtatcattga ctacaaggcc tttaactttg tggacggcga aggcgtgcgt aactctctct
    26881 atgtatcagg ctgtatgttt cactgcgagg gatgttataa tgttgcgact tggtctttta
    26941 atgctggcat tccctataca gcagaattag aagagcagat catggcagat cttgctcagc
    27001 cctatgttca aggtttgact ttgttgggag gggagccttt tcttaatact gggattctct
    27061 tgccacttgt taagcggatt cggaaggaat tgccagacaa ggacatctgg tcctggaccg
    27121 gctacacttg ggaagaaatg atgttggaaa ctccagataa actggaattc ttgtcactga
    27181 ttgacattct tgtcgatgga agatatgatc gaactaagag aaatcttatg ctccagtttc
    27241 gaggttcatc taaccaacga attatcgatg tgcaaaaatc gctcaaaagt gggcaagtag
    27301 tgatttggga caagctcaat gacggaaaag aaagctatga acaggtgaag agagaatgaa
    27361 gaaaaaatga agaaaaaaga cttagtagac caactagtct cagagatcga gacggggaaa
    27421 gtcaggacac tgggaatata cggtcatgga gcttcaggta aatcaacctt tgcacaggaa
    27481 ttgtaccaag ctttagattc tactacagta aaccccaatt at
//
LOCUS       DAVLXF010000020        26988 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLXF010000020 DAVLXF010000000
VERSION     DAVLXF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.020"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            131..679
                     /locus_tag="VYO12_000787"
     CDS             131..679
                     /locus_tag="VYO12_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1587428.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            789..1688
                     /locus_tag="VYO12_000788"
     CDS             789..1688
                     /locus_tag="VYO12_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1587429.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1713..2987)
                     /gene="serS"
                     /locus_tag="VYO12_000789"
     CDS             complement(1713..2987)
                     /gene="serS"
                     /locus_tag="VYO12_000789"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1587430.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3201..3572)
                     /locus_tag="VYO12_000790"
     CDS             complement(3201..3572)
                     /locus_tag="VYO12_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1587431.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKVSRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3575..4939)
                     /locus_tag="VYO12_000791"
     CDS             complement(3575..4939)
                     /locus_tag="VYO12_000791"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1587432.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREIGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYYAFFGE
                     SKED"
     gene            5309..6094
                     /locus_tag="VYO12_000792"
     CDS             5309..6094
                     /locus_tag="VYO12_000792"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1587433.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6188..6622
                     /locus_tag="VYO12_000793"
     CDS             6188..6622
                     /locus_tag="VYO12_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1587434.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6622..7596
                     /locus_tag="VYO12_000794"
     CDS             6622..7596
                     /locus_tag="VYO12_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1587435.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7656..7880
                     /locus_tag="VYO12_000795"
     CDS             7656..7880
                     /locus_tag="VYO12_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1587436.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            8000..8974
                     /gene="fabK"
                     /locus_tag="VYO12_000796"
     CDS             8000..8974
                     /gene="fabK"
                     /locus_tag="VYO12_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1587437.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            8967..9887
                     /gene="fabD"
                     /locus_tag="VYO12_000797"
     CDS             8967..9887
                     /gene="fabD"
                     /locus_tag="VYO12_000797"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1587438.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9921..10652
                     /gene="fabG"
                     /locus_tag="VYO12_000798"
     CDS             9921..10652
                     /gene="fabG"
                     /locus_tag="VYO12_000798"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1587439.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            10674..11909
                     /gene="fabF"
                     /locus_tag="VYO12_000799"
     CDS             10674..11909
                     /gene="fabF"
                     /locus_tag="VYO12_000799"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1587440.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11912..12397
                     /locus_tag="VYO12_000800"
     CDS             11912..12397
                     /locus_tag="VYO12_000800"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1587441.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12394..12816
                     /gene="fabZ"
                     /locus_tag="VYO12_000801"
     CDS             12394..12816
                     /gene="fabZ"
                     /locus_tag="VYO12_000801"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1587442.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            12828..14195
                     /gene="accC"
                     /locus_tag="VYO12_000802"
     CDS             12828..14195
                     /gene="accC"
                     /locus_tag="VYO12_000802"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1587443.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVKKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14232..15098
                     /locus_tag="VYO12_000803"
     CDS             14232..15098
                     /locus_tag="VYO12_000803"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1587444.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15095..15862
                     /locus_tag="VYO12_000804"
     CDS             15095..15862
                     /locus_tag="VYO12_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1587445.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            16021..16206
                     /gene="briC"
                     /locus_tag="VYO12_000805"
     CDS             16021..16206
                     /gene="briC"
                     /locus_tag="VYO12_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1587446.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            16224..16697
                     /locus_tag="VYO12_000806"
     CDS             16224..16697
                     /locus_tag="VYO12_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587447.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            16710..16946
                     /locus_tag="VYO12_000807"
     CDS             16710..16946
                     /locus_tag="VYO12_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1587448.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(16996..17418)
                     /gene="nusB"
                     /locus_tag="VYO12_000808"
     CDS             complement(16996..17418)
                     /gene="nusB"
                     /locus_tag="VYO12_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1587449.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQMVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17411..17800)
                     /locus_tag="VYO12_000809"
     CDS             complement(17411..17800)
                     /locus_tag="VYO12_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1587450.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(17822..18382)
                     /gene="efp"
                     /locus_tag="VYO12_000810"
     CDS             complement(17822..18382)
                     /gene="efp"
                     /locus_tag="VYO12_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1587451.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(18511..19953)
                     /gene="gatB"
                     /locus_tag="VYO12_000811"
     CDS             complement(18511..19953)
                     /gene="gatB"
                     /locus_tag="VYO12_000811"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1587452.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNTEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(19953..21419)
                     /gene="gatA"
                     /locus_tag="VYO12_000812"
     CDS             complement(19953..21419)
                     /gene="gatA"
                     /locus_tag="VYO12_000812"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1587453.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQTTLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLSAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(21419..21721)
                     /gene="gatC"
                     /locus_tag="VYO12_000813"
     CDS             complement(21419..21721)
                     /gene="gatC"
                     /locus_tag="VYO12_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1587454.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            21934..23478
                     /locus_tag="VYO12_000814"
     CDS             21934..23478
                     /locus_tag="VYO12_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1587455.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNKRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            23906..24104
                     /locus_tag="VYO12_000815"
                     /pseudo
     CDS             23906..24104
                     /locus_tag="VYO12_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            24215..24391
                     /locus_tag="VYO12_000816"
     CDS             24215..24391
                     /locus_tag="VYO12_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1587456.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            24507..24695
                     /locus_tag="VYO12_000817"
     CDS             24507..24695
                     /locus_tag="VYO12_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1587457.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            24852..25217
                     /locus_tag="VYO12_000818"
     CDS             24852..25217
                     /locus_tag="VYO12_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1587458.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            25220..26887
                     /locus_tag="VYO12_000819"
     CDS             25220..26887
                     /locus_tag="VYO12_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1587459.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         7764 a         5123 c         6218 g         7883 t
ORIGIN      
        1 gtgtgataaa ctaatataac aacgaaaatc ttatgagaga ataaagcttt atcttagata
       61 aacttaggaa tcaggatttt ataagatttt ccaataatat tagtgtcaat aataagaaaa
      121 gaggtatctt atgacaacat ttactatcca tacagtagag tcagcaccag cagaagtgaa
      181 agaaattctt gaaacagtag aaaaagacaa caatggctat attcccaacc taatcggtct
      241 cttggccaat gccccgactg ttttagaagc ctaccaaatt gtctcatcta tccaccgtcg
      301 caacagcctg acacccgttg agcgtgaagt ggtgcaaatc acggcagccg tgaccaatgg
      361 ttgtgccttc tgtgtcgcag gtcacacagc cttttccatc aaacaaatcc agatgaatga
      421 tgacttgatt caagctcttc gcaatcgtac tccaattgaa acagatccta aattggatac
      481 cctagctaag tttaccttgg cagttatcaa taccaagggt cgtgtaggag atgaagcctt
      541 gtctgagttt ttagaagctg gctacactca acaaaatgcc ttggatgtgg tttttggtgt
      601 cagcctagca atcctctgta actatgccaa caacttagct aatacaccaa ttaatccaga
      661 attgcaacct tatgcctaat tcatatctga gtaaaatgaa gtctgaaata atcggacttc
      721 gtttttttaa atcctcattt aagcgctttt atgttatact gaaactaaca tgattattgg
      781 aggttaggat gaaaaaactc cccttagtat tttcaggttg tttgctaggt ttggcaggag
      841 ctggaaatct tattttagat acgttgccgg ttctatccca tctttttagt ctgactggtt
      901 tggttttatg gatttacttt ctaattctgc atctctttaa ttggaaagaa accaagcaag
      961 aattgaccaa gccccctctt ttgtcaggaa tggcaacctt tcctatggct gggatgattt
     1021 tatcgaccta tgtctttcgc gtattctctt atcttccttt ggtagcacaa gggatttggt
     1081 ggttttcatt tctcttggat ttgaccttga ttgctggttt taccatcaag tttgcttgtc
     1141 cagggcggag ggttcatgcc actccaagct ggacggttct ctatgtgggg atagcagtgg
     1201 ctgccttgac ctatcctctg gtaggtatta tcgaaattgc ctatgcgacc ttgagttttg
     1261 gttttctctt gaccttctat ctctatcccc ttatttatag cgatttaaag aaacatccac
     1321 tcccactagc cttgcttgga caagaaggaa tctactgtgc tcctttctct ctactcttgg
     1381 cttctctagt tcgagtagga ggaaccagcc tgccgacttg ggtcttgatt gtcatgattt
     1441 tggcttctca atccttcttt ttctttgttt taactcgtct gcccaacatt ttaaaacaag
     1501 gttttcaacc agccttctca gccctcacct tcccaaccat tatcacagcg acctcgctca
     1561 agatggctca gggaattttg aaacttccat ttctggatta cctggtattg gctgaaacca
     1621 ttatatgcct aactatttta ttctttgtac taggtgctta tctgatttgg ttacgaaaaa
     1681 aggtctagct agaaatagct aaaccttatt ttttatggtt tgatgacttc agctccaccc
     1741 atgtatggac gaagtgcttc tgggatggtc acagaaccat cttcattttg gtaattttca
     1801 agaattgcag ccactgtacg tccaactgca agtccagaac cgttcaaggt atgaaggagt
     1861 ttcaccttgc catctgcttc atcacggtaa cggatttggg cacgacgggc ttggaaatct
     1921 tctgtgtttg aacagcttga gatttcacgg taattgtttt gtgctggaat ccacacttcc
     1981 aagtcgtaag tcttcgcagc tgagaagccc atatctccag tagagagagc aacgacacgg
     2041 tatggaaggt tgagtttttg aagaatgttt tcagcgttgg ctgtcatttt ttccaattct
     2101 tcgtaagatt cttctggttt ggcaaatttg accatttcaa ccttgtggaa ttggtgcaaa
     2161 cggatcaagc cacgcgtatc acgaccggca gaaccagcct cagaacggaa tgacggactc
     2221 atggcagtga agtagattgg aagatctttg ccgtctaaga tttcatcacg gtagtagttt
     2281 gtcagaggaa cttcagcagt tggaatcaag acaaagttgg tatcgctgag ttcaaaagta
     2341 tcttccttaa attttggata ctgaccagta ccaaacatag aatcatggtt gactatgtaa
     2401 ggtgtgatga cttcagtata gccttctttt ccatgttcat ccaacataaa gttgtagata
     2461 gcacgttcca aacgagcacc gaggccttta tagaagagga agcgagcgcc tgttacctta
     2521 ccaccgcgtt cccagtcaag gataccaagg tcttcaccga gatcccagtg agctttaggt
     2581 tcgaagtcaa actcgcgtgg agtaccccaa cggcgaactt ccacattgtc gtcttcgtca
     2641 gtcccaacag gaacgctgtc agctgggata tttggaagag tcgttgtaaa ttctgtcaat
     2701 ttagcatcga tttctgccaa ttcagcatcc aaggctttaa cctcagcaga tagattttgc
     2761 atggcagcaa tcttgtcatc tgtattttcc ttgttgcgct tagcttgggc aatctcagca
     2821 gaaactgtgt tacgttctgc tttgagagtt tcaaccttga ccaagatgtt acgacgttta
     2881 gcatcgattt ctttcatttc attcaagaca gcagcatcta caccacgtgt agctaatttt
     2941 tctgcgacag cttcaaaatc tgtacgaata cgtttgatat ctaacataag aactccttta
     3001 tgaaaaaagc acgcctgaca aaacgttgga gtggcagggc cacggttcca tccaacttca
     3061 caggtgtgca cttgattgtg tatgtaattg ttactaacgg tagaatttca cctatccccc
     3121 ctatctgctc gcagtacccg cagactttct gaaagaagaa gataacctac ttatccgttg
     3181 ctatgattat actaaagttt ctactttttt gcaaatagat ttttaaattt ttggctaatt
     3241 gtctgaatca gggtcggaag tttgacgacc ttgtcattgc ctagtttttc gcgtgcaatt
     3301 ttgagaatgg cacctgagtc ttttgaagca aagaggaatt ttcctttgtc tgtaaagact
     3361 tcgaagtggc ggctgacttt gcgtccagtg acattggctc caatctgatt gatatggctc
     3421 caaggaatct ggataaattg ttcgacattg acatctgggt aaaattccaa agcctgatct
     3481 ccgacaagga atttcccaac tttcccagcg atagagaggt aggaagtgcc tgtcgtactg
     3541 aggagtactg ttttgttaag tgattgagcc atgcttagtc ttccttactt tctccaaaaa
     3601 aggcatagta gagggcttta attgctgctt tctcttggtc tttattgaca acaaacataa
     3661 tagaaacttc actagaacct tgagacatca tctggatgtt gattttgttt tcagatagag
     3721 cgcgtgtcgc agtagcagtc actccgatat ggctcttcat tttttcacca acaatcataa
     3781 tgatagaaag gtcgtgttcg atttctgcat gatctacttt agccttttga accaactgac
     3841 gcaggatttc ttcttccttg atgggagtta gttggcgaga acggagaatg atagaaagat
     3901 cgtcgatacc tgttggcata tgttcccaac cgatgttaag ttcttccagg atttgcagaa
     3961 ccttgcggcc aaatccaatc tcacggttca tgaggtattt cgacatatta atgctgacaa
     4021 agcctgagtc accagcaatt cccacaactg gaaattcatc attactgtgt tttagaacga
     4081 tacgagtacc tggatggtca gggttgttgg tattcttgat aaccagagga atttttccac
     4141 ggtaggcagg aagaagagcc tcgtcatgaa ggactgagaa gcctgcatag gccaactcgc
     4201 gcatttcacg gtaggtcaac tcaggaatcg agtgtggttg gtggataata ccagggtggg
     4261 ctgcaaagat accatcaacg tccgtaaagt tttcatagag gtcagctttg acaccagcag
     4321 caatgataga acctgtaata tcagatcctc cacgtgagaa agtacagatt tgattttcct
     4381 tagtgacacc aaagaaacca ggaatgacaa ggacttcatt ggtgtttgtc aattcttcaa
     4441 tcttgtcata acttgatgga atgatgcgag cgtgaccagg ttcacttgtg accacaatcc
     4501 cagcttctct agggtgcata tagcgtgcat cgataccatt ttggttaaag taggcagcaa
     4561 tcaatttggc attgttattt tcaccggctg ctaggaaagt atcgtagaga aattcatttt
     4621 cttcaatagg aagagtggcc aaggcgtgaa tgcttttaga aattttttct agcacagctg
     4681 gttttagtcc caattcacta accatagcag catagcggtc gataatccag ctttggctct
     4741 tgctaatatc gttacccgca acatagtcgc ggtagtattt aatcagggca tccgtaacct
     4801 tagtatcttc agcattgcgt ttaccaggcg cagaaacgac tacaaaacga cgctctgaat
     4861 cgcttttgac gatgtttaaa actttttcta attgactagc agaggcaaga gaactacctc
     4921 caaatttaac aactttcata agaactccta aagtaagtat tttatacgat tatagcagaa
     4981 agaaaggctt ttttcaatga agaaaataag atgctttcta gcctaaagcg agcctttctt
     5041 atcggctgag acactttcag tcgatttttt cttgctttct gctcctaaaa aagatatact
     5101 ttgacagtga aataatttaa cactcaatga aaatcaaaga gcaaactagg aagctagccg
     5161 cagactgctc aaagcactgc tttgaggttg taaataagac tgacgaagtc agctcaaaat
     5221 actgttttga ggttacagat ggaagctgac gcggtttgaa gagattttcg aagagtataa
     5281 actgcttata aaataaaaag gagccctgat ggaacacatt atttatcagc ttgaagagga
     5341 cttggcaatc cttaccttga accgtcctga ggtcgcaaat ggttttcata ttcccatgtg
     5401 tgaggagatt ttagaagctc tgactttggc agaagaaaat ccagctgtgc attttatctt
     5461 aatcaatgcc aatggaaagg tcttctcagt tgggggagat ttggtagaga tgaagcgggc
     5521 agtggatgag gatgatattc catcattgac aaaaatcgca gagttggtca atacgatttc
     5581 ttataaaatc aagcaaatag ccaaacctgt tttaatggaa gttgacggtg ctgttgcagg
     5641 tgccgcagcg aatatggctg ttgcggcaga tttctgtttg gcgacggata aggctaagtt
     5701 tatccaagcc tttgttggtg ttggtttggc tccagatgca gggggaattc atctcttgag
     5761 ccgcagtatc ggtgtgacgc gtgctgctca attagctatg acaggagagg ctttaacggc
     5821 agaaaaagcc ctagaatggg ggttggttta ccgcgtttct gaagctgaaa aacttgaaaa
     5881 gacgagagaa cagcttctta aaaaattaag acgtgcttca agtaattcct atgctgccat
     5941 taagaagttg gtttgggaga gccaatttaa agactggcag ggttacgcta ctttagaact
     6001 gaacctacag aaatccttag ctcaaacaga ggatttcaaa gaaggagtgc gagctcattc
     6061 ggagagaaga agacctaaat ttacaggaaa ataaaaaata cttgcatcat tctttgaatt
     6121 ttgatataat tctttctgtc aaatgttttg attgtaaaag ttttttgaag aaggagggat
     6181 atgaaaaatg gactaccaac gaatcaatga atatttaaca tctatattta acaatgtcct
     6241 tgtaattgag gaagtgaact tgagaggtag tcgttttaag gatatctcca tcaaagaaat
     6301 gcatacgatt gatgtcatcg gtaaggctcc agatgtgact ccaagtcaag tgtcaaaaga
     6361 gttgatggta actcttggaa ctgttacgac aagtttgaac aatttagagc gtaagggcta
     6421 cattgagcga gttcggtcag aacaggatcg tcgtgtggtg catctgcatt tgacaaagaa
     6481 gggtcgcttg attcatagac tgcataaacg cttccacaag gccatggtag aaaaaattat
     6541 tgatggcatg agcgaggaag aaattgctgt catgggtaaa ggcttgacta atctttacca
     6601 atttttggag gatttgaaat aatggctttt gcaaaaataa gtcaggttgc tcattatgtg
     6661 ccagagcaag tggttacaaa tcacgacttg gctcagatta tggataccaa tgatgagtgg
     6721 atttcaagtc gaacgggaat acgacaaagg catatttcaa gaacagaatc taccagtgat
     6781 ttggctacag aggttgctaa gaaactgatg gcaaaagctg gaataacagg aaaagaactg
     6841 gattttatca tcctagctac cattactcca gattcgatga tgccctctac agctgctcgt
     6901 gttcaagcta atattggtgc taataaagcc tttgcttttg acttaaccgc ggcttgcagt
     6961 ggatttgtat ttgctctttc aactgctgaa aagtttatcg cttctggtcg ctttcaaaaa
     7021 ggcttggtga ttggtagtga aaccctctct aaggcagtcg attggtcgga tcgatcaaca
     7081 gctgtgttgt ttggagatgg tgctggtggt gtcttgttag aagctagcga gcaagagcat
     7141 ttcttagctg agagtctcaa tagcgatgga agtcgcagcg agtgtttaac ttatgggcat
     7201 tcaggtttgc attctccatt ttcagatcaa gaaagtgcag attcattttt gaagatggat
     7261 ggacgcacag tctttgattt tgccattcga gatgtagcca agtctatcaa gcagactatt
     7321 gatgaatctc ctatagaggt gacagacttg gattatctgc tacttcatca agccaatgac
     7381 cgtattttgg ataagatggc tagaaaaatt ggtgttgacc gagccaaact tccagccaat
     7441 atgatggaat atggcaatac cagtgcagcc agtatcccga ttttactttc agagtgtgta
     7501 gaacaaggtc tcatcccttt agatggtagc cagactgttc ttctatcagg cttcggtgga
     7561 ggtttgacct ggggcacgct cattcttaca atttaggtaa tcatgtggtg aacacattgt
     7621 tataaaaaaa ctatttattt taaaggagtc ctatcatggc agtatttgaa aaagtacaag
     7681 aaattatcgt tgaagaactt ggaaaagacg catcagaagt aacacttgaa tcaacttttg
     7741 atgatttgga cgcagattca ttggacttgt tccaagtaat ctcagaaatc gaagatgctt
     7801 ttgatatcca aatcgaagca gaaaatgact tgaaaacagt tggtgacttg gttgcttacg
     7861 ttgaagagca agcaaaataa gcagaatatt aagtagaagg agtagggaga aacccactcc
     7921 ctcttgttta ggtaatagtt tgactgtcaa attatggtga aatcgaacta ttacgtaagc
     7981 aaggaaggtt ggaggcacta tgaaaacgcg tattacagaa ttattgaaga ttgactatcc
     8041 tattttccaa ggagggatgg cctgggttgc tgatggtgat ttggcagggg ctgtttccaa
     8101 ggctggagga ttaggaatta tcggtggggg aaatgccccg aaagaagttg tcaaggccaa
     8161 tattgataaa atcaaatcat tgactgataa accctttggg gtcaacatca tgctcttatc
     8221 tccctttgtg gaagatatcg tggatctcgt tattgaagaa ggtgttaaag ttgtcacaac
     8281 aggagcagga aatccaagca agtatatgga acgtttccat gaagctggga taatcgttat
     8341 tcctgtcgtt cctagtgttg ctttagctaa acgcatggaa aaaatcggtg cagacgctgt
     8401 tattgcagaa ggaatggaag ctggggggca tatcggtaaa ttaacaacca tgactttggt
     8461 gcgacaggta gccacagctg tatctattcc tgttattgct gcaggaggaa ttgcggatgg
     8521 tgaaggtgct gcggctggct ttatgctagg tgcagaggct gtacaggtgg ggacacggtt
     8581 tgtagttgca aaagagtcga atgcccatcc aaactacaag gagaaaattt taaaagcaag
     8641 ggatattgat actacgattt cagctcagca ctttggtcat gctgttcgtg ctattaaaaa
     8701 tcagttgact agagattttg aactggctga aaaagatgcc tttaagcaag aagatcctga
     8761 tttagaaatc tttgaacaaa tgggagcagg tgctctagcc aaagcagttg ttcacggtga
     8821 tgtggatggt ggctctgtta tggcaggtca aatcgcaggg cttgtttcta aagaagaaac
     8881 agctgaagaa atcctaaaag atttgtatta cggagccgct aagaaaattc aagaagaagc
     8941 ctctcgctgg acaggagttg taagaaatga ctaaaacagc ctttttattt gctggtcaag
     9001 gtgcccagta tctagggatg ggacgggatt tctatgatca gtatccgatt gttaaagaaa
     9061 cgattgatcg agcgagccag gtactaggtt atgatttgcg ttatctcatc gatacggaag
     9121 aagacaaact caatcagacc cgctatacgc aaccagccat tctagcgact tcggttgcta
     9181 tctaccgttt attgcaagaa aagggctatc agcctgatat ggttgctggt ttgtctcttg
     9241 gagaatactc tgccttggtg gcaagcggcg ccttggattt tgaagatgcg gttgccttgg
     9301 tagctaagcg tggagcctat atggaagaag cggctcctgc tgactctggc aagatggtag
     9361 cagttctcaa tacgccagta gaggtcattg aagaagcctg tcaaaaagct tctgaacttg
     9421 gagtggttac tccagccaac tataacacac ctgcacaaat cgtcattgct ggagaagtgg
     9481 ttgcagttga tcgagcggtt gaacttttgc aagaagcagg tgccaaacgc ttgattcctc
     9541 ttaaggtgtc aggtcccttt cacaccgctc tccttgagcc tgctagccag aaactagctg
     9601 aaactctagc tcaggtaagt ttttcagatt ttacttgtcc cctagtcggc aatacagaag
     9661 ctgctgtgat gcaaaaagag gacattgctc agctcttgac gcgtcaggtc aaggaacccg
     9721 ttcgtttcta tgaaagtatt ggggtcatgc aagaagcagg cataagcaac tttatagaga
     9781 ttggaccggg gaaagtcttg tcaggctttg ttaaaaaaat tgatcaaact gctcacttag
     9841 ctcatgtgga agatcaagcg agtttagtag cacttttaga aaaatagact aaaataagta
     9901 gaagttttga aaggaaaaaa atgaaactag aacataaaaa tatctttatt acaggttcga
     9961 gtcgtggaat tggtcttgcc atcgcccaca agtttgctca agcaggagcc aacattgtct
    10021 taaacagtcg tggggcaatc tcagaagaat tgctcgctga gttttcaaac tatggtatca
    10081 aggtggttcc catttcagga gatgtatcag attttgcaga cgctaagcgt atgattgatc
    10141 aagctattgc agaactgggt tcagtagatg ttttggtcaa caatgcaggg attacccaag
    10201 atactcttat gctcaagatg acagaagcag attttgaaaa agtgctcaag gtcaatctga
    10261 ctggtgcctt taatatgaca caatcagtct tgaaaccgat gatgaaagcc agagaaggtg
    10321 ctatcattaa tatgtctagt gttgttggtt tgatggggaa tattggtcaa gctaactatg
    10381 ctgcttctaa ggctggcttg attggcttta ccaagtctgt ggcacgcgag gtcgctagtc
    10441 ggaatatacg agtcaatgtg attgctccag gaatgattga gtctgatatg acagctatct
    10501 tatcagataa gattaaggaa gctacactag ctcagattcc gatgaaagaa tttgggcagg
    10561 cagagcaggt tgcagatttg acagtatttt tagcaggcca agattatcta actggtcaag
    10621 tgattgccat tgatggtggc ttaagtatgt agcgaaagct agaggtgaaa agaatgaaac
    10681 taaatcgagt agtggtaaca ggttatggag taacatctcc aatcggaaat acaccagaag
    10741 aattttggaa tagtttagca actgggaaaa tcggcattgg tggcattaca aaatttgatc
    10801 atagtgactt tgatgtgcat aatgcggcag aaatccaaga ttttccgttc gataaatact
    10861 ttgtaaaaaa agataccaac cgttttgata actattcttt atatgccttg tatgcagccc
    10921 aagaggctgt aaatcatgcc aatcttgatg tagaggctct taatagggat cgttttggtg
    10981 ttatcgttgc atctggtatt ggtggaatca aggaaattga agatcaggta cttcgccttc
    11041 atgaaaaagg acccaaacgt gtcaaaccaa tgactcttcc aaaagcttta ccaaatatgg
    11101 cttctgggaa tgtagccatg cgttttggtg caaacggtgt ttgtaaatct atcaatactg
    11161 cctgctcttc atcaaatgat gcgattgggg atgccttccg ctccattaag tttggtttcc
    11221 aagatgtgat gttggtggga ggaacagaag cttctatcac accttttgcc atcgctggtt
    11281 tccaagcctt aacagctctc tctactacag aggatccaac tcgtgcttcg atcccatttg
    11341 ataaggatcg caatgggttt gttatgggtg aaggttcagg gatgttggtt ctagaaagtc
    11401 ttgaacacgc tgaaaaacgt ggagctacta tcctggctga agtggttggt tacggaaata
    11461 cttgtgatgc ctaccacatg acttctccac atccagaagg tcagggagct atcaaggcca
    11521 tcaaactagc cttggaagaa gctgagattt ctccagagca agtagcctat gtcaatgctc
    11581 acggaacgtc aactcctgcc aatgaaaaag gagaaagtgg tgctatcgta gctgttcttg
    11641 gtaaggaagt acctgtatca tcaaccaagt cttttacagg acatttgctg ggggctgcgg
    11701 gtgcagtaga agctatcgtc accatcgaag ctatgcgtca taactttgta ccaatgacag
    11761 ctgggacaag tgaagtatca gattatatcg aagctaatgt cgtttatgga caaggcttgg
    11821 agaaagaaat tccatacgct atttcaaata cttttggttt tggaggccac aatgcagttc
    11881 ttgctttcaa acgttgggag aatagataag tatgaattta aacgatatta aagacttgat
    11941 gactcaattt gaccagtcaa gtttgagaga attttcttat aaaaatggga cggatgagtt
    12001 gcagtttagc aagaatgaag caagacctgt gcctgaagtt gcaactcaag tcgctccagc
    12061 acccgttcta gcaacaccga gtccagtagc tcctacatct gctccagcag agactgtagc
    12121 agaagaagtt ccagctccag ctgaagcaag tgtggctact gagggaaatc ttgtagagag
    12181 tccacttgtt ggagtggttt acttggctgc tggaccagat aaacctgcct tcgttacagt
    12241 tggtgatagt gtcaaaaaag gtcaaacatt ggtaattatc gaagccatga aagtcatgaa
    12301 tgaaatccca gctcctaagg atggtgtggt aacggaaatt ctcgtctcta acgaagaaat
    12361 ggttgagttt ggtaaaggat tggtacgtat caaatgatcg atattcaagg aatcaaagaa
    12421 gctcttcccc accgttatcc tatgcttcta gtggaccgtg tcttggaagt gagcgaggat
    12481 accattgttg ctatcaaaaa tgtgaccatt aacgaacctt tctttaacgg ccactttcct
    12541 caatacccag ttatgccagg tgttctgatt atggaagcct tggcgcaaac tgccggtgtg
    12601 ttggagttat caaaacctga aaataaagga aaactggtct tttacgctgg tatggacaag
    12661 gttaagttca agaagcaagt tgtaccaggc gaccaattgg ttatgacagc gacttttgta
    12721 aaacgtcgtg gcaccatagc tgtggttgaa gcaaaggctg aagtggatgg caagcttgca
    12781 gccagtggta cccttacttt tgcaattggg aactaaggag gttctccatg tttcgaaaaa
    12841 ttttaattgc caatcgtggt gaaattgcgg ttcgtattat ccgtgcggca cgtgaattgg
    12901 ggattgcgac ggtagcggtt tattcaactg ctgataagga agctcttcat acgcttttgg
    12961 cagatgaagc agtttgtatt ggtcctggca aggcaacaga gtcttatctc aatattaatg
    13021 cagttctatc agctgcagtc ttgactgagg cagaagctat tcaccctggt tttggatttc
    13081 tcagtgaaaa ttccaaattt gcgaccatgt gtgaagaagt aggtatcaag tttatcggtc
    13141 catctggtca tgttatggat atgatggggg ataagatcaa tgcgcgtgct cagatgatta
    13201 aagcaggtgt gcctgttata ccaggttcag atggagaagt gcataactct gaagaagctt
    13261 tgattgttgc tgaaaaaatt ggctatcctg ttatgctcaa ggcttcagca ggtggaggtg
    13321 gtaaagggat tcgtaaggtt aaaaaaccag atgacctcgt ttctgccttt gaaactgcct
    13381 ctagtgaggc caaggccaat tatggcaatg gtgccatgta catagaacgg gttatctatc
    13441 cagctcggca cattgaggtt caaatcctag gtgatgagca tggacatgtg attcacttgg
    13501 gtgaacggga ttgttctctt caaaggaata accaaaaggt tttggaagaa agtccctcga
    13561 ttgcaatcgg aaaaacgctg cgtcatgaaa taggtgctgc tgctgttcga gcggcagagt
    13621 ttgttggcta tgagaatgca ggaaccattg aatttcttct tgatgaagca agtagcaatt
    13681 tctatttcat ggagatgaat actcgtgttc aggtagaaca tccagtaaca gagtttgttt
    13741 caggtgttga tatcgttaag gaacagattt gcattgcggc aggtcagcct ttgtctgtta
    13801 agcaagaaga tattgtccta cgcggtcatg ccatcgagtg tcgtatcaat gcagaaaacc
    13861 cagcctttaa ctttgctcca agtccaggta agattactaa tctctatctg ccaagtggtg
    13921 gagttggctt gcgcgtggat tcagcagttt atccaggtta taccattccg ccttattatg
    13981 atagtatgat tgccaaaatc atagtacacg gcgaaaatcg ttttgacgcc ttgatgaaaa
    14041 tgcaacgtgc cctctatgaa ttagagattg aaggagtgca gaccaatgca gatttccagc
    14101 ttgacctcat ttcagatcgc aatgtcattg ctggggatta tgatacttcc ttcttgatgg
    14161 aaaccttctt acctaaatat caagaaaaag aataaaatga ctttaagagt ttaaaccgaa
    14221 aaggggaatc aatggctcta tttagtaaaa aagataagta tattcgaatc aatcccaatc
    14281 gttcggttag ggaaaaacct caagctaagc cagaggttcc agatgaatta ttttcccagt
    14341 gtccaggctg taagcatacc atctatcaga aggatctggg aagtgaacgt atctgtccgc
    14401 actgtagcta tacctttcgt atttctgccc aagaacgctt ggctttgacg attgatatgg
    14461 gaaccttcaa agaattgttt acagggattg aaagcaagga tcccttgcat ttccctggtt
    14521 accaaaagaa actggcatct atgcgtgaaa aaacaggtct gcatgaagcc gttgtgacag
    14581 gaactgctct tattaaaggt cagactgtgg ctcttgggat tatggattct aactttatca
    14641 tggcttctat gggtacggtt gtaggtgaaa aaatcactcg tttgtttgag tatgcgactg
    14701 tcgaaaaatt gccagttgtc ctattcacag cctctggtgg agcccgtatg caggaaggaa
    14761 tcatgagtct catgcagatg gctaagatct ctgcggcggt taaacgccat tcaaatgctg
    14821 gtctctttta cctgaccatt ttgacagatc caacgactgg tggtgtgaca gcttctttcg
    14881 ctatggaagg cgatatcatt ctggctgaac cacagagctt ggttggtttt gctgggcgtc
    14941 gtgtgattga aaatacggtt cgtgaaagct tgcctgagga tttccaaaag gcagaattcc
    15001 tattagaaca tggctttgtg gatgctattg tcaaaagaag agacttacca gatacgattg
    15061 ctagcctagt cagattgcat ggagggagtc ctagatgaat attgcaaaaa tagtcagaga
    15121 agcgcgtgag cagagtcgct tgacaacctt ggactttgcg acaggcattt ttgatgaatt
    15181 tatccaatta catggtgacc gttcttttcg tgatgatggt gcagttgttg gtggtattgg
    15241 ttggcttgga gatcaagctg taacagtggt tggtatccaa aaaggcaaga gtttgcaaga
    15301 caacctcaaa cggaattttg gccaaccaca tccagaaggc taccgaaagg cactgcggtt
    15361 gatgaaacag gctgagaaat ttggccgtcc agttgtgacc tttatcaata cagcaggtgc
    15421 ttatcctggt gtcggagcgg aagaacgtgg tcaaggggaa gctatcgctc gcaatctcat
    15481 ggaaatgagt gacctgaaag ttcctattat cgccattatt atcggtgaag gtggttcagg
    15541 cggggctctg gctctagctg tcgcggaccg tgtctggatg ctggaaaatt ctatctatgc
    15601 cattctcagt ccagaaggct ttgcttccat tttatggaag gacggtactc gcgctatgga
    15661 agcagcagaa ctgatgaaaa tcacttcgca tgaactgtta gaaatggacg tggtggataa
    15721 ggtgatttct gaaataggac tttctagtaa agaactgatt aagagtgtca aaaaagaact
    15781 ccaaacggag ctggctagac tttcacaaaa accgctagaa gagttgctgg aagaacgcta
    15841 tcaacgattt agaaaatact aagaatgact aaatgagact agaaaggtct cattttttat
    15901 ggaaaaatgt aattcaggtc gaaaaattgc cagttgagga gatgggctat aaaatagaca
    15961 aaaaaagaaa tatactataa atgtgaacct acaacaatct attttaagaa ggtacttgca
    16021 atgacaggta caaatacatt tacagttctt tcaactgagg acttggagca aacttcaggt
    16081 ggtcttgctg tttgggaaga tggatatagt agatggttat attatagaga atttgctccc
    16141 tatatgaggc aaggggcact taattcttat atagatgctt ggaagtacgg cttccgagca
    16201 gggtaatctt tcaaaaaagt acgatgagaa acacaaaaaa actaagacaa tttggaatat
    16261 ttttgcttat tattttacta tctacctatt taccacaaac aattggattg tatgtgacga
    16321 tcattttagg gttaggagct gatgtctatt ctcttatttt aacaatggga ttagtgggaa
    16381 gtttccttct attaatttgg aggttaaaaa agaaaaagat gctctttatc tttgagaaaa
    16441 agagctggaa ctggtcattt gtcttctact tatttgcaac ttatgtggtt tatcagattc
    16501 tcggtaattt ttgggcacgc tatgctcatt tgattaatca taggaatatt catgatgagt
    16561 attttaccgt gcttttttca aatggacaac caaccttttt atcgactatc ttatcttttg
    16621 tcctgcctgt aatcatcggt cctgtttttg aggagactct tgatagaggg tattttatga
    16681 ataccttctt tccctagtcc aagtactatt tggatgttat cctatctgct atcatctttg
    16741 ggattagcca tttgatacta tctcacagag atccaattag tttattatat tatagtctga
    16801 ttggtttctt ttttgccctt gtttatcgtt ctaccgacaa tctgagatta acgattctct
    16861 gccatagttt ttttaacttt ctcaatcatg caaaacctat ctggattttt gtttacaatt
    16921 atatctatta tcattttttt agatagtggg aggcaaagaa aaaagtgttt gatatctatc
    16981 aaacactttt tctttttatt gttcttctgt tacaaactgg ctgagcagtc cattgataaa
    17041 acgggcagat ttttgatcgg agaagtcctt tgcaagctcg atagcttcat taacagcaac
    17101 catctgagga gtgtcaaatg aagtgatttc aaagactccc aagcgaagga ggtttctctc
    17161 cacgagcgtt aagcgttcaa tggtccaacc tgcttttaaa tgctgagtga tttgcttatc
    17221 tagttcttcc tttttagctt gaacaccaga aacgaggtct atcaaaaagg ctggaagttg
    17281 tacatccgta tcttcacgat catgagtata ggcgaaacga caagcagttt cgacatccgt
    17341 accgaactca aggctcatga gagcttgaaa agcgcattta cggagttggc gtctagattc
    17401 taatagtgga ctagtcattg aggaagtcct cgtcaaatag atctttcaat tctggttttg
    17461 gtgttttatc tgggacgata cctgcaacgt gaatattgat agcagcgagt tctacatcag
    17521 ccatattacg gacggcatct ttgacagctt tctggatagc aaccgctacc ttaggaactt
    17581 ttactccgta ctcaaggtag agatagatat ctgctgtgag ttcttcgtcc acgtttttaa
    17641 gataaatgcc acggccgagt gaaagttttg aaagggtatc agacactgat ctgtttgaaa
    17701 aagagtgaac accctctacc tttgcagtag cgatagcaat gattttttca agtacacgtg
    17761 gggcgataac gatttcgcca agttgttctt caattcccat agaatgacct ctttctagag
    17821 attaggcacg agaaacgtaa gttccttctg cagtgttgat aacgagtttt tgtcctgctt
    17881 cgatgaagtc tggaacgttt acgacaagtc cagtttccat cgttgctggt ttaccagaac
    17941 ctgtaacagt agcacctttg atagatggtt gagtttcagc aactgtcaac tcaacagtag
    18001 taggaacggt gacaccgatc acttcagttc cgtagaattg gattttcaca tcagagtttt
    18061 caaggatgta aagcaattcg ttttcaacat tgactacagg gatttcgtat tggtcgtaag
    18121 tttctgtatt catgaagtat gctgtgtcat ccattttgta caagtattga gctgggacag
    18181 tctcgataat agcttgttca aatttttcct ctggacggta gcttgtgtca aatgtagaac
    18241 cagtacggac atcacgcaat ttcatacgca tgatcgtgtt tcctttacct ggtttgtggt
    18301 gactagcttc caaaacgcga atcaatttgc cgtcagctgt ttcaaaggtc ataccagctt
    18361 ttaatttact tgcttcaatc atgtttttac ctctttaata aatattatta ctcttcattt
    18421 taccataaaa tactctggaa ataaagccaa aatagttatt gggagttggg agagaagaaa
    18481 aaaccaacct tagggctggt tttgttctgt ctagttttct ttcaacttcg ccaattcctg
    18541 tgcaagtagt ttaagggcaa cttgtgggtt ggcttggcct ttggttgcct tcataaggaa
    18601 tcctgtaaag gccttgtcgg cgttacgttt gcctgacttg aagtcggcaa cagcagcttc
    18661 gttatcggca aagacttggt ggatgattgg gatcaagata gctggatctg aaatttgaac
    18721 catacctgct ttttccacgt attcacgcgc gccaccgcca tttttagcta gatggacaaa
    18781 gactttcttg gcaatcttag atgaaatagt accgtcttcg atgatggcaa tcatttcaac
    18841 caagttttct ggtgtcaatt cgatttgttc cagtgtttta ccttcagtat tcaagaactg
    18901 agcgacttcc ccttggagcc agttagagac ttgtttggca tcaccaccta gggcaacagc
    18961 tttttcaaag aagtcagaag tgactttatt agcagtcaac tgactagcat cgtagtctga
    19021 taaaccaagg tcagatacat aacgcgcacg acgttctttt ggaaactctg gcaactcagt
    19081 ccgcatttcc tcaatccact cgtcagaaat ttcaaagagg ggtaggtctg gttctgggaa
    19141 gtagcggtag tcagcagccc cttccttgac acgcatgagg atggttgctt tattcgcttc
    19201 atcgtaacgg cgtgtttctt ggcggatttg accacctgag cgaagaattt cagcctggcg
    19261 ttggacttcg tattcaagac ctttacgaac gtttgagaag gagttgaggt tcttcaattc
    19321 agtcttggta ccgaatttct cttgaccata aggacgaagg gagatgttgg catccacacg
    19381 catcgaacct tcctccatct taacgtcaga aatgccagcg tactggataa cttccttgag
    19441 ggctgtcaga taagcatagg cttcttcagg agaacgcata tctgcctcag atacaatctc
    19501 aatcaaggga accccttggc ggttgaggtc aacataagag tagccatctg taccatgggt
    19561 gtttttacca gcgtcttcct ctaggtgggc acgttcgata ccgattttct tggtcgtacc
    19621 gtcttctagt tcgacttcaa tccagccatt atatccgatt ggttcatcaa attgagaaat
    19681 ttggtaggct ttggggttat caggatagaa gtagttcttg cggtcaaagt gcatcttttt
    19741 gtggatgtcc atgttgaggg caagagcagc cttgattccg gcatcgacaa cccctttatt
    19801 gagaactggc agaactcctg ggaaagacca gtcaatcacg ttagtgttgg cattttgatc
    19861 atttccgaag tgggcagaag taggtgagaa gatttttgaa ttggtgttga gctctacgtg
    19921 gacttcaagt ccaatgactg tttcaaagtt cattagttgt cacctccaaa aatcacgggt
    19981 tgttgtttgt ggtagtctgt tgttgcttca aaagcagcag cagcttggta aatggtttcc
    20041 tcagagtact tgggaccaat caattggagt ccgacaggta gaccttgaga gaatccagca
    20101 ggaatcgaaa ttccaggcag tcctgccaag tttacaggta tggtcaatag gtcggctaag
    20161 tacatggcaa ctgggtcatg gttgagagaa tccaagtcat aggcaacact tggagcagtt
    20221 ggacccaaaa tcaaatcgta atccgcgaag actttttcga aatcttgaat gataagtgta
    20281 cggacctgtc cagccttctt gtagtaggca tcgtagtaac ctgatgaaag actgaaagta
    20341 cccagcatga tacggcgctt cacttcttca ccaaaacctt ggcttcggct gtttacatag
    20401 atttcatcaa ggttggttgc atcttctgcg cgatagccgt aacggatacc gtcaaagcgt
    20461 tgcaagtttg atgaagcttc tgatgaagcg atgatgtagt atacggcaac tccgtattta
    20521 gagtgaggaa ggctgacttc ttcgacaata gcacccaatt tttcaaagtg tttggccgcg
    20581 tttaagattg tttccttaac ttctggatca attccttcac cgaggtattc cttaggcaaa
    20641 gcgattttca tacccttgat gtcttggccg atttttgaag taaagtcggc gatgcgaaca
    20701 ggagcagaag tagagtcttt agcatcttcg ctggcaatag cgctgagcaa gagggcattt
    20761 tccttaacag taggagcaaa aggtccaatc tggtctaatg agctaccaaa ggcaatgaga
    20821 ccgaaacgtg aaactgttcc gtaggttggt ttgagaccaa cgattccgtt gaaggcagca
    20881 ggttggcgga tggaaccacc agtatcagaa ccaagtgaca agcgaacttg tcctgaggct
    20941 acagctgcgg cagaaccact tgatgaccca ccaggaacct tgctgtggtc ccaagcgttt
    21001 ttagttgctc cgtagtgtga agtttcacct gaaccaccca tagcaaattc gtccatgttg
    21061 gtctttccaa cgacaatcat gcccttggtt tttgcattgg caacagctgt cgcatcaaag
    21121 attggctcat agttgtagag catttttgag gcagcagttg tgagaatacc gtctgtagag
    21181 atgttatcct taacagcaag tggaattcct gaaaggacat tgtcagcatc aattccagct
    21241 tcatcaatgg ctttagcttg aacaagagct tgctcctcag cgatggtgac aaatgaattg
    21301 agagcttcct cacgagactt gatattttca agtgttgttt gggtcaattc tgttgcagaa
    21361 atttccttag agacaaggag attgtgcaac tcttcaatag ttttattgtt aaaagtcatt
    21421 aggcatctcc tccatcgtct aggatagctg gcaccttgat atagtagttg tctttttcag
    21481 gtacgttttt aaacaagcga tcacggtctg ttccttcttc ggccacatca gggcggagta
    21541 cagtcttgcg gtcagccata gtcgtagtag gtgcgacacc agttgtgtca acttcgccca
    21601 gcaattcaac catgtcaaca atcttagaca aggtggtcgc aaaggcagca gtttcttttt
    21661 cagagaatct taattttgaa agattggcaa cgtgtgttac ctcttcttgc gtaattttca
    21721 tcttgcatcc tttcgtgaaa tgatgatttt tagtctgttc tattttacca tattttccta
    21781 taaataaggg agggggaagt gaaaagaaat agaaattgaa aaaaatgcta aaatccgata
    21841 taatggagtg ttgaaaggaa tggtaaaatc caatgtaaaa atcattctca gctattgaaa
    21901 caagaaaaat agagaatcaa agaaagagaa cttatgaata ttcaagaaga aattaagaaa
    21961 cgtcgtacct ttgccattat ctcccacccg gacgcgggga aaacaaccat cactgagcag
    22021 ttactctact ttgggggtga aattcgtgag gctggtacgg taaaagggaa gaaaacaggg
    22081 acttttgcta aatctgattg gatggatatc gagaagcaac gtgggatttc tgttacttca
    22141 tctgttatgc aatttgacta cgacggcaag cgcgtgaata tcttagacac gccagggcac
    22201 gaggacttct cagaagatac ctatcgtacc ttgatggcgg tggatgctgc ggtcatggtc
    22261 gtggactctg ccaaggggat cgaggctcaa accaagaaat tgtttgaggt tgtgaaacat
    22321 cgtggcattc cagtctttac ctttatgaac aagctggacc gtgacggtcg tgagcctttg
    22381 gatctcttac aagaattgga agaaatcttg ggcattgcta gctaccctat gaactggcct
    22441 atcgggatgg ggaaagcctt tgagggcttg tatgacctct ataacaaacg tttagagctt
    22501 tataaagggg atgagcgttt tgctagtcta gaagatgggg acaaactttt tggtagcaat
    22561 cctttctacg agcaagtcaa ggatgatatt gagcttttaa atgaagccgg gaatgagttt
    22621 tcagaggaag ctattctggc tggagaattg acgcctgtct ttttcggttc agccctgaca
    22681 aactttggtg tgcagacctt ccttgaaacc ttcctcaagt ttgctccaga accacatggt
    22741 cacaagaaaa cagacggtga aattgtggat ccttatgaca aggatttctc aggctttgtc
    22801 tttaaaatcc aagccaacat ggaccctcgt caccgtgacc gtattgcctt tgtccgtatc
    22861 gtatctggcg aatttgagcg cggcatgagt gttaatctcc ctcgtactgg taagggggct
    22921 aaactgtcta atgttaccca gtttatggcg gaaagtcgtg agaatgtgac caatgccgtg
    22981 gcaggtgata ttatcggggt ttacgataca ggcacttatc aggttgggga taccttgaca
    23041 gttgggaaaa acaaatttga atttgaacca ttgccaacct ttactcctga aattttcatg
    23101 aaagtttctg ctaagaatgt catgaagcaa aaatccttcc acaaggggat tgagcaacta
    23161 gtgcaagaag gagccgttca gctttataag aattaccaaa caggtgagta catgctaggc
    23221 gctgtaggtc aactccagtt tgaagtcttt aagcaccgta tggaaggaga atataatgcg
    23281 gaagtggtca tgagcccaat gggtaaaaag accgttcgtt ggatcaagcc tgaggatttg
    23341 gatgaacgga tgtcatcaag tcgcaatatc ttagccaaag accgttttga ccaaccagtc
    23401 tttctctttg aaaatgactt tgccctccgc tggtttgcgg acaagtatcc agacgtagag
    23461 ttagaggaga agatgtgact cagtaccaac aattggaact aaagttccaa attgttggac
    23521 gctagtcgct atttggcgaa ctagctaact gcctcactaa ctacgtttgg caaatagaat
    23581 cgatttgcca aactccgtgt cgtaatctaa taatcggtaa ccgctatggc ggcttaccta
    23641 aacgcttcac taggaaaaat ccacgaatgg tattgatttg tggatttttc cgtcgtagca
    23701 gaaagtaggt agcctagcta actgccttac taactacgtt tggcaaatga aatcgatttg
    23761 taaaactccg tgtcgtaatg taaaaattgt aaaccgtaat gggacttcat ctaaaatctc
    23821 cactggtatg gttttacgaa cgaatgggcg aaaataatgg ttacgtagaa acatatgatg
    23881 ggaataaaaa gaaatgagat aaagagtgaa agaagagaaa aaagcgattg tcttaggtgc
    23941 agataatgct tatatggata aagttgaaac aacaataaaa tctctttgtg ttcatctatt
    24001 ataatcttaa gttttatgtt tttaatgatg accttccgcg agaatggttc caattgatgg
    24061 aaaaaagact agagactttg aatagtgaga ttgtgaatgt ttagattgac agtagtattc
    24121 tgaaaggtta tcgtctcccc tttgagggac tttcatatgc ggctttttta ggtattttat
    24181 tccaaagtat gtaagcgagt ctcgtgtact gtatttggat ttagatattg ttgtaagaaa
    24241 atcgatagat gagctgtggg atttggactt gacggctata cccttagctg cagttagaga
    24301 tgatttttat acacataatt ttaattctgg tgttctgtta atcaacaatg gcatgtggag
    24361 agcagaaaat gtcacgcaag acctgattta agacacaaat aaaaaatctt ccaattccct
    24421 tgcaaaaaac aagaaagtgt gataacatag tacggtatgt ggtgctagca catccgctat
    24481 attagatcta ataggaggaa aacacaatgg ctaaagtatg ttactttaca ggtcgtaaga
    24541 ctgtatcagg aaacaaccgt tcacacgcga tgaaccaaac aaaacgtgcc gtaaaaccaa
    24601 accttcaaaa agttactgtt cttatcgatg gtaaacctaa aaaagtttgg gcttcagctc
    24661 gtgctttgaa atcaggtaaa gttgaacgcg tttaataata aaagcaaagg agaccttagg
    24721 gtcttttttc ttttattgtg ggtataacat cgtttgaaaa atagagtaaa tatccgctga
    24781 tacatcattc tgcttttaca cttgggctga aatatgataa aatagagtat caactagttg
    24841 aggtaaaaag gatgactgta aaaattaata caaaagatgg tcaaatcgaa ctaacagatg
    24901 aagtgattgc aaccgtcgta ggtggagcag caactgagat ttttggtgtg gtcggtatgg
    24961 ctagtaaaaa tgccctcaaa gataatttcc aagccctgct aggtaaggaa aattattcta
    25021 aaggtgtcgt cgtaaaggca gccgaagatg gcagtattgc agttgatgta tataccgtgt
    25081 taagctacgg aacaaagatt agtgaagtgt caaaaaacat tcaagagcgt gttgccttta
    25141 gcttggaaaa ccagcttgga attactgctc agactgtaaa tgtctacatt caaaatatca
    25201 aagttgtagg agaataaccg tgtcaaaaat tactactagc ttatttcaag aaatggtgca
    25261 ggctgcatca actcgcttga ataagcaagc tgaatatgtc aattcattaa acgtctttcc
    25321 agttccagat ggagatactg ggacaaatat gggaatgacc attgaaaatg gtgctaaaga
    25381 agttgcagac aagccagctt ctacagttgg agaagtagcg agcattcttg ccaaagggct
    25441 tttgatgggt gcgcgtggga actcaggagt gattacgtct cagcttttcc gtggattttc
    25501 ccaagctatc aaggataaag acgagttaac aggtcaagac ttggctctgg ccttccaatc
    25561 aggtgtggaa gttgcctata aggcagtgat gaaacctgtt gaaggaacga ttttaacagt
    25621 ttctcgtgga gctgctatcg gtgctaagaa aaaagctgag caaacagatg acgctgttga
    25681 agtcatgcgc gcagccttgg aaggtgctaa aacagctcta gctaaaacac cagacatgct
    25741 tccagtattg aaagaagttg gcgttgtgga ctcaggtggt caaggattgg tcttcatcta
    25801 cgaaggtttc ctttcagccc ttactggcga atatattgca tctgaggact ttgtagcgac
    25861 tcctgccaac atgagtgaga tgatcaatgt agagcatcat aagtctgtag ctggtcacgt
    25921 agcgactgag gacatcacgt ttggttactg tactgaaatc atggtagctc ttaagcaagg
    25981 tccaacctat gctaaagatt ttgactacga tgaattccgt aactacttgg atgagcttgg
    26041 ggattctctc ctcgttgtta acgatgatga aattgtcaaa gtccatgttc atacagaaga
    26101 tccaggactt gttatgcaag aaggtctcaa atatggtagc ttggtcaagg taaaagttga
    26161 caatatgcgt aaccaacacg aagcacaggt tgagaaagaa gctactcaag ttatcaagtc
    26221 ggctgaagaa aaagagtatg ctttgattgc tgtggtggct ggtaaaggtc tagcagatat
    26281 cttctgttct caaggcgtgg attatgttat cgaaggcggt caaaccatga acccttcaac
    26341 agaagacttt atcaaggctg ttgaacaggt caatgcccgt aacatcatct tcttgccaaa
    26401 caacaagaac atcttcatgg cagctcaatc tgcggcagaa gttttggagc aaccagcagt
    26461 agtggtagag gctcgcactc ttcctcaagg tatgacaagt cttcttgcct ttgatccaag
    26521 caagtccatt gaagaaaacc aagagcgtat gacagctgct cttagcgatg tcgttagcgg
    26581 aagcgtcaca acagccgtgc gtgatacaac gatcgatggc ttagaaatcc atgaaaacga
    26641 taatctaggt atggtggatg gaaaaattct tgtgtcaaac cctgatatgc accaaacatt
    26701 gactgaaacc ttgaaacata tgttggatga agatagtgaa atcgtaacct tctatgtcgg
    26761 tgaagacgga agcgaagaac ttgccaatga aatcgctcaa gaaatcgtag aagaattcga
    26821 agacgttgaa gtcgagattc accaaggtca acaacctgtt tacccatacc tatttagtgt
    26881 ggaataagat taatgaagga ctgagaaatc agttcttttt tcttttttag taaatgaaat
    26941 cggtatcttt ttaataaaaa caaaaataac attcataaat aaacgtta
//
LOCUS       DAVLXF010000021        26079 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLXF010000021 DAVLXF010000000
VERSION     DAVLXF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.021"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            205..2184
                     /locus_tag="VYO12_000820"
     CDS             205..2184
                     /locus_tag="VYO12_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1587460.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2251..3747
                     /locus_tag="VYO12_000821"
     CDS             2251..3747
                     /locus_tag="VYO12_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587461.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3747..4673
                     /locus_tag="VYO12_000822"
     CDS             3747..4673
                     /locus_tag="VYO12_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587462.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4682..5749
                     /locus_tag="VYO12_000823"
     CDS             4682..5749
                     /locus_tag="VYO12_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587463.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5760..6686
                     /locus_tag="VYO12_000824"
     CDS             5760..6686
                     /locus_tag="VYO12_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587464.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            7253..8113
                     /locus_tag="VYO12_000825"
     CDS             7253..8113
                     /locus_tag="VYO12_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1587465.1"
                     /translation="MSQFITHQSLYATREGFVDKLAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQINLIKKVLPKAKTIGILYTQSEPNSVVQKDEAKRLLKEKGFT
                     VVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQVSIKHKVPVFGGSTE
                     MIAVGGLYNYGTNYEELGRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            8125..9018
                     /locus_tag="VYO12_000826"
     CDS             8125..9018
                     /locus_tag="VYO12_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587466.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            9031..9834
                     /locus_tag="VYO12_000827"
     CDS             9031..9834
                     /locus_tag="VYO12_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1587467.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMNLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(9867..10577)
                     /gene="treR"
                     /locus_tag="VYO12_000828"
     CDS             complement(9867..10577)
                     /gene="treR"
                     /locus_tag="VYO12_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1587468.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            10762..12729
                     /gene="treP"
                     /locus_tag="VYO12_000829"
     CDS             10762..12729
                     /gene="treP"
                     /locus_tag="VYO12_000829"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1587469.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATNPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            12907..14532
                     /gene="treC"
                     /locus_tag="VYO12_000830"
     CDS             12907..14532
                     /gene="treC"
                     /locus_tag="VYO12_000830"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1587470.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMTDYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            14788..15036
                     /locus_tag="VYO12_000831"
     CDS             14788..15036
                     /locus_tag="VYO12_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1587471.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            15243..16037
                     /gene="racE"
                     /locus_tag="VYO12_000832"
     CDS             15243..16037
                     /gene="racE"
                     /locus_tag="VYO12_000832"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1587472.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            16034..17005
                     /locus_tag="VYO12_000833"
     CDS             16034..17005
                     /locus_tag="VYO12_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1587473.1"
                     /translation="MTNKIYEYKDDRDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            16981..17502
                     /locus_tag="VYO12_000834"
     CDS             16981..17502
                     /locus_tag="VYO12_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1587474.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            17499..17960
                     /locus_tag="VYO12_000835"
     CDS             17499..17960
                     /locus_tag="VYO12_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1587475.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            17951..18691
                     /gene="xerD"
                     /locus_tag="VYO12_000836"
     CDS             17951..18691
                     /gene="xerD"
                     /locus_tag="VYO12_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1587476.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            18691..19419
                     /locus_tag="VYO12_000837"
     CDS             18691..19419
                     /locus_tag="VYO12_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1587477.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            19438..20007
                     /locus_tag="VYO12_000838"
     CDS             19438..20007
                     /locus_tag="VYO12_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1587478.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            19994..20716
                     /locus_tag="VYO12_000839"
     CDS             19994..20716
                     /locus_tag="VYO12_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1587479.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVVNKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            20716..20958
                     /gene="yidD"
                     /locus_tag="VYO12_000840"
     CDS             20716..20958
                     /gene="yidD"
                     /locus_tag="VYO12_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1587480.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(21183..22148)
                     /locus_tag="VYO12_000841"
     CDS             complement(21183..22148)
                     /locus_tag="VYO12_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1587481.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(22244..22996)
                     /locus_tag="VYO12_000842"
     CDS             complement(22244..22996)
                     /locus_tag="VYO12_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1587482.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            complement(22993..23949)
                     /locus_tag="VYO12_000843"
     CDS             complement(22993..23949)
                     /locus_tag="VYO12_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1587483.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSFFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(23939..24898)
                     /locus_tag="VYO12_000844"
     CDS             complement(23939..24898)
                     /locus_tag="VYO12_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587484.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(25321..26022)
                     /locus_tag="VYO12_000845"
     CDS             complement(25321..26022)
                     /locus_tag="VYO12_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEV1587485.1"
                     /translation="MTKTILVTGGTSYISSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNAAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
BASE COUNT         7502 a         5021 c         5902 g         7654 t
ORIGIN      
        1 cctaagctcc aaaatttctg tgtcaaattt cctattttca ctgtagtctt tgacacttcg
       61 aacgacaatt tgaatcatat attcaaaaaa aatgaaaaat ttgtgccaac aagcctaaat
      121 attctgaaaa tttgtttgat ttttaatgga taatgtgata taatggttgt caatctataa
      181 ttcacataaa cagaggagta gaaaatgaaa aaaaatcgtg tatttgctac agcaggtctt
      241 gttttattag cagcaggtgt acttgcagca tgcagttctt caaaatcatc tgattcatca
      301 gcccctaaag cttatggcta tgtttataca gcagacccag aaaccttgga ctacctgatt
      361 tcaagtaaaa atagtacaac agtagtgact tcaaatggga ttgatggttt attcactaac
      421 gataattacg gtaatcttgc tcctgcagtt gcagaggatt gggaagtctc taaggatggt
      481 ttgacctaca cttataagat tcgtaaaggg gttaaatggt ttacctctga tggagaagaa
      541 tatgcagagg tgacggctaa agatttcgtg aacggtttaa aacacgcagc agataaaaaa
      601 tcagaagcta tgtatttagc tgaaaattcg gttaaaggct tggcagatta tctatcagga
      661 acttcaacag atttttcaac agttggtgtc aaggcggttg atgattatac gttacaatac
      721 actttgaacc agcctgaacc gttctggaac tctaagttga cctattctat tttctggcct
      781 ctgaatgaag aattcgaaac atcaaaagga agcgattttg ctaaaccaac agatccgaca
      841 tccttgcttt ataatggtcc attcttgttg aaagggttga ctgcaaaatc ttctgtagag
      901 tttgtaaaaa atgagcaata ttgggataaa gaaaatgtcc acctagatac tatcaatcta
      961 gcttactatg atggatcaga tcaggagtcg ctagagcgta acttcactag tggagcttat
     1021 agttatgccc gtctttaccc taccagctcc aactattcta aggttgcaga agaatacaag
     1081 gacaatatct attacacaca atcaggctct gggattgctg gtctgggtgt gaatattgat
     1141 cgccaaagtt acaactatac ttctaaaact acagattcag agaaagtagc tactaagaag
     1201 gcattgctta acaaagattt ccgtcaagcc ttgaattttg ctcttgatcg ctcagcttac
     1261 tcagctcaaa tcaatggtaa agatggagca gctttagcag ttcgtaattt atttgtaaaa
     1321 ccagactttg tttcagctgg tgagaagacc tttggtgatt tagtcgctgc tcaacttcct
     1381 gcttatggtg atgagtggaa aggtgtgaat ttagctgatg ggcaggatgg tttattcaat
     1441 gctgacaagg ccaaggcaga gtttgcgaaa gctaagaaag ctttagaagc agacggcgtt
     1501 cagtttccta ttcatctgga cgttccagta gaccaagcat caaaaaacta tatatctcgt
     1561 attcagtcct ttaaacaatc tgtagaaaca gttcttggtg ttgaaaatgt cgttgttgat
     1621 attcaacaaa tgacaagtga tgaattcctt aatattactt actatgctgc caatgcttca
     1681 tctgaggatt gggatgtatc gggaggagtt tcatgggggc cagactatca agacccatct
     1741 acttacctgg atattttaaa aacaactagc agtgaaacta caaaaacata tttaggattt
     1801 gataatccaa atagcccttc agtagttcaa gttggtttga aagaatacga taaattagtt
     1861 gatgaagctg ccaaagagac aagcgacttg aatgtccgtt atgaaaaata tgcagcggct
     1921 caagcatggt tgacagatag ttcactcttt attcctgcta tggcttcttc tggtgcagca
     1981 ccagtgcttt cacgaattgt tccatttact ggagcttctg cgcaaacagg ctctaagggg
     2041 tcagatgttt acttcaaata tttgaaatta caagataaag cggtgactaa ggaagagtat
     2101 gaaaaagctc gtgaaaaatg gttgaaagaa aaagctgaat caaatgagaa agctcaaaaa
     2161 gaattggcaa gtcatgtgaa gtaaataatt tctaatagaa ctttctctcc atgaggagaa
     2221 ggttcttttg ggatttttaa aggaaataat atgaaaaaat atatttttat gcgtgttttg
     2281 cggtcattgg tttcgatttt cttagtaacg actttgacct acacgattat ctataccttg
     2341 gttcctcgaa aattgatttt caagcaggat cctaactata ataaaattgc gacaacggct
     2401 gataaacgtg ataactatga aaatactgtg tttgagcgta tgggctacat tgagtattac
     2461 gatactaaag agttgcaaga aaaggcaagt agcatggatt cttctgtaac agtagaagca
     2521 aatgcgacca ataaagctat ttatgaaaag tacatcaatc aattaggtca tggttggact
     2581 ttgggagaat ttactgaaag tggtcaattc tatgctactc gtgaaattcc aatttttgaa
     2641 cgtgtttttc acttctatgc taacttgatt gacattgacc atacaaataa aatccaagac
     2701 cctgaaaatc cagacttgaa acgctacctt cgttttgaaa atgatccagc tatcggatgg
     2761 tcattggtcg gttcaggaac taaacataaa tatctcttgt actttaacag tcagttccca
     2821 tttgttcatc aaaactttgt gaacttgaat ttaggtgact cttacccaac ctatgctaat
     2881 acaccagttc ttcaggttat tactcaaggt caaggacaaa ccaaaactgc ccaagttcag
     2941 ttcccaacag gtaagaaaac gtcttctgta aatatttact caagaaccta caagtcacct
     3001 agtcaggctg actctcgtga agtagctagc tatgggaaag atgatcctta tacagcgact
     3061 gaaagtaatt accaatatcc atctatgatt gtcagctctg ctattactgg tttgattggt
     3121 ttggttcttg cctatgctct tgccgtgcca cttggttcag ccatggctcg tttcaagaac
     3181 acttggattg atagcctctc aacaggggct ttgaccttct tgcttgctct tccaacgatt
     3241 gccttggttt acatcgttcg attgattgga tcatctattt cccttccaga ttcattccct
     3301 atcttgggag ctggagattg gcgttcttac gttttaccag cagtcatcct tggtttgttg
     3361 ggtgctcctg gtacagccat ttggattcgt cgttacatga ttgacttgca atctcaagac
     3421 tttgttcgtt tcgctcgtgc aaaaggtttg tctgaaaaag aaatttcaaa caaacacatc
     3481 tttaaaaatg ccatggttcc gctggtttca ggaattcctg gtgccattat tggggttatc
     3541 ggtggtgcaa cccttactga aacagtcttc gccttcccag gtatgggtaa aatgttgatt
     3601 gactctgtaa aagcatctaa taactctatg gtcgttggtc ttgtcttcat ctttacatgt
     3661 atttctatct tctcacgtct tttgggagat atttggatga ctattattga cccacgtatt
     3721 aaattgactg agaaaggagg caaataatgt ctacaatcga taaagaaaaa tttcagtttg
     3781 taaaacgtga cgattttgcc tctgaaacta ttgatgcgcc agcatattct tactggaaat
     3841 cagtgtttaa acaatttatg aagaaaaaat caactgtagt catgttggga atcttggtag
     3901 ccatcatttt gataagtttc atctacccaa tgttttctaa gtttgatttc aatgatgtca
     3961 gcaaggtaaa cgactttagt gttcgttata tcaagccaaa tgcggagcat tggttcggta
     4021 ctgacagtaa cggtaaatcg ctctttgacg gtgtctggtt cggagctcgt aactccatcc
     4081 tcatttctgt gattgcgaca gtgattaact tggttatcgg tgtttttgtc ggtggtattt
     4141 ggggtatttc aaaatcagtt gaccgtgtca tgatggaagt ttacaacgtc atctcaaaca
     4201 tcccacctct tttgattgtt attgtcttga cttactcaat cggagctgga ttctggaatc
     4261 tgatttttgc catgagcgta acaacatgga ttggtattgc cttcatgatc cgtgtgcaaa
     4321 tcttgcgcta tcgtgacttg gaatacaact tggcgtcacg tactttggga acaccaacct
     4381 tgaagattgt tgccaaaaat atcatgcctc aattggtatc tgttattgtg acaaccatga
     4441 ctcaaatgct tccaagcttt atctcatacg aagccttctt gtctttcttc ggtcttggat
     4501 taccgattac agtgccaagt ttgggtcgtt tgatttcgga ttattcacaa aacgtaacaa
     4561 ccaatgctta cttgttctgg attccattga caacccttgt cttggtatcc ttgtcccttt
     4621 tcgtagttgg tcaaaactta gcggatgcta gtgatccacg tacacataga taggagtaga
     4681 aatgacaaaa gaaaaaaatg taattttgac tgctcgcgat attgtcgtgg aatttgacgt
     4741 tcgtgacaaa gtattgacag ccattcgcgg cgtttccctt gaactagtcg aaggagaagt
     4801 attagccttg gtaggtgagt caggatcagg taaatctgtt ttgacaaaga ccttcacagg
     4861 tatgctcgaa gaaaatggtc gcattgccca aggtagtatt gactaccgtg gtcaggactt
     4921 gacagcttta tcttctcaca aggattggga acaaattcgt ggtgctaaga ttgcgactat
     4981 cttccaggac ccaatgacta gtttggaccc cattaaaaca attggtagtc agattacaga
     5041 agttattgta aaacaccaag gaaaaacagc taaagaagcg aaagaattgg ccattgacta
     5101 catgaataag gttggcattc cagacgcaga tagacgtttt aatgaatacc cattccaata
     5161 ttctggagga atgcgtcaac gtatcgttat tgcgattgcc cttgcctgcc gacctgatgt
     5221 cttgatctgt gatgagccaa caactgcctt ggatgtaact attcaagctc agattattga
     5281 tttgctaaaa tctttacaaa acgagtatca tttcacaaca atctttatta cccacgacct
     5341 tggtgtggtg gcaagtattg cggataaggt agcggttatg tatgcaggag aaatcgttga
     5401 gtatggaacg gttgaggaag tcttctatga ccctcgccat ccatatacat ggagtctctt
     5461 gtctagcttg cctcagcttg ctgatgataa aggggatctt tactcaatcc caggaacacc
     5521 tccgtcactt tatactgacc tgaaagggga tgcttttgcc ttgcgttctg actacgcaat
     5581 gcagattgac ttcgaacaaa aagctcctca attctcagta tcagagacac attgggctaa
     5641 aacttggctt cttcatgagg atgctccaaa agtagaaaaa ccagctgtga ttgcaaatct
     5701 ccatgataag atccgtgaaa aaatgggatt tgcccatctg gctgactagg aggaaggaaa
     5761 tgtctgaaaa attagtagaa atcaaagatt tagaaatttc cttcggtgaa ggaagtaaga
     5821 agtttgtcgc ggttaaaaat gctaacttct ttatcaacaa gggagaaact ttctcgcttg
     5881 taggtgagtc cggtagtggg aaaacaacta ttggtcgtgc tatcatcggt ctaaatgata
     5941 caagtaatgg agatatcatt tttgatggtc aaaagattaa tggtaagaaa tcgcgtgaac
     6001 aagctgcgga attgattcgt cgaatccaga tgattttcca agaccctgcc gcaagtttga
     6061 atgaacgtgc gactgttgat tatattattt ctgaaggtct ttacaatcac cgtttattta
     6121 aggatgaaga agaacgtaaa gagaaagttc aaaatattat ccgtgaagta ggtcttcttg
     6181 ctgagcactt gactcgttac cctcatgaat tctcaggcgg tcaacgtcaa cgtatcggta
     6241 ttgcccgtgc cttggtcatg caaccagact ttgttattgc agatgagcca atttcagcct
     6301 tggacgtttc tgtacgtgcc caagtcttga acttgctcaa aaaattccaa aaagagctcg
     6361 gcctgaccta tctcttcatc gcccatgact tgtcggttgt tcgctttatt tcagatcgta
     6421 tcgcagttat ttacaagggt gttattgtag aggttgcaga aacagaagaa ttgtttaaca
     6481 atccaattca cccatatact caagccttgc tttcagcggt accaatccca gatccaatct
     6541 tggaacgtaa gaaggtcttg aaggtttacg acccaagtca acacgactat gagactgata
     6601 agccatctat ggtagaaatc cgtccaggtc actatgtttg ggcgaaccaa gccgaattgg
     6661 cacgttatca aaaaggacta aactagtaat ggttttataa tttccatgtc aatttttatg
     6721 gaaattataa aacttttttt gttggactga tttgaaaaac tttgaaagct tcctgaaaga
     6781 atttttgaaa aattttaaaa attatgaaat gttattgaca ggtgatgaaa aaggtggtag
     6841 gacaggaagc atcaaaaaag aaagcagaaa aagaaatgaa taagagacaa gccgtaacta
     6901 tgaatcaaaa cttttacttt agctacttta gatagtgttt gtagacatgt caccatggat
     6961 gcaaacagtt caagcaattt gtttgtatct atgtggctaa gatttttgat tgtggtccat
     7021 atagatgaat atctaaatgg actagctaag ttgtaacttg ttaggttatt tcaagtatcc
     7081 gtttagatag tatctaggcg gttttttgtt ttatcttttc tgagaaggag tttcattatg
     7141 aaagttgttc gaaaatcact agccccctct tggtagtggg gctcctcttg acttctctga
     7201 tcagtttgca tcagttgaag gcagataaga aaaagatgtc tttcgtatcg gtatttcgca
     7261 atttattacc caccagtcct tatacgctac tagagaagga tttgtggata agctggccaa
     7321 acaaggctat gttgaggggg aaaatattga gattgatttg caaaatgcac agggagaaca
     7381 aagaaatcta aaaacgattt cccagcaact agcagaatcc agcgatgtcg ttctagccat
     7441 cgcaaggcct tctgctcaga gcttggccaa tacgacgcaa acgacaccag ttatcttttc
     7501 agctgtaaca gacccagtca gtgccaagtt ggttgagtca agagaacatc ctgggggcaa
     7561 tgtaactggg acaagcgatc agtcatcaga tgccatttca acccaaatca atttgataaa
     7621 gaaagtgttg ccaaaggcta aaacgattgg aatcctctat actcagagcg agcccaattc
     7681 ggttgttcaa aaggatgaag ctaagcgact tctgaaagaa aaaggcttta ccgttgttga
     7741 aaaaacaatc ttggacagta ataacgtcaa ggcggcagca gagagtttga tggcagaggt
     7801 ggatatggtt tttgtcccaa ctgataatat catttcgtta accatggaaa cagttaagca
     7861 ggtttctatt aaacacaagg ttccagtatt cggtggttca acagaaatga ttgctgttgg
     7921 tggcttgtat aactacggaa ccaattatga agaattggga cgtcagacag ctcgcatgct
     7981 gattcgtgtt ttaaaaggtg agaaaccaga aaatatagca gttgagttgc ctgaaaaact
     8041 ggaattgcat accaatcaag aaatggcaga tgcattggga attgatatta gtaagttaga
     8101 aggcaaggaa taaggaggat taagatgaat tttgtgttat ctagtttatc agaaggtttg
     8161 ttgtggtcga ttgtggcgat tggggtctac ttgactttcc gtattttgga tattgcggat
     8221 atgactgctg agggagcctt tcctctgggg gcagcagtcg ttgtatcaca gatacaggca
     8281 ggggcaaatc cttggcttgc gactttactt gctttgctgg caggtatggt agcaggtctt
     8341 gtatcaggaa tgctccacac caagatgaaa atcccagctc tcttgacagg gattgtgacc
     8401 ttgacagggc tctattcaat caatattaaa atcatgggca gtgtacccaa tctttccttg
     8461 ggagattctg caaccgtctt taaacagttg gcgagcttgg gtctgacaaa tgaaggaact
     8521 gttttctcac ttagtttggc ctgtttttta cttgtttgtt tggtcttgac ccttttgatg
     8581 aaaacagaga ttggcttggt cttgcgttcg actggggata atatcccgat gagtgaggcc
     8641 aatggagtca atgtagacac catgaagatt gttggttaca tgatttcaaa cggtttgatt
     8701 gctctatgtg gttccttgtt tgcccaaaat gatggatttt cagatgtgac ttctgggaca
     8761 ggaaccattg ttgttggttt gagctcggtg attattgcgg aagtcttgat acacgacttg
     8821 accattggag gccgcttatt atccatcgga atcggtgcta ttgtttaccg tttgattatt
     8881 ttaaatatct atgaaattcc aaatctagat caaaacttgg tacgtctctt taatgcaatc
     8941 ttgctcgctt tggttttatt tgcaccagaa ttgcaaaaga gattaaagat tcgtggtctg
     9001 aaattgagaa atgaatagga ggagaaagtt atgacaagtt tgttaacact tgaaaatatt
     9061 cacaagacat ttgaagcagg gacggtcaat gagaaccatg tcctcaaagg attagattta
     9121 gaggttgaag agggagattt tatctctgtg attggtggaa atggagcagg aaaatccact
     9181 ttgatgaata tcttggctgg caatctatcg gtggacgaag gggaccttct actggcaggg
     9241 aaatccatta aaaatctgag tgtcagaaag agagccaagg atattgcccg tgtttttcaa
     9301 gatcctaaga tgggaacagc ttctcgtttg acgattgagg agaatatggc tattgccttg
     9361 agacgcgggc agaagagagg gcttggttgg ggcgtgaagg agaaggatag aatccagttc
     9421 caagaggcct tgaaggagtt gaatattggt cttgaaaacc gcttaaaagt agatactcaa
     9481 tatctctcag gaggacaaag acaggctttg actctagtca tgacagatct gatgaaacca
     9541 aaacttttac ttttggatga acatactgcg gcacttgacc cgaaaaccag tcaaatggtg
     9601 atgaacttga cgcaaaagat tgtggagcac catcagtgga cgactttgat gattacccat
     9661 gatatgaatc atgcgattga gtacggcaat cgtctcatta tgctctatca aggcaagata
     9721 gtggtggatg tcaagggaga agagaaaaag catctgacag ttgaagacct tatgcatctc
     9781 ttccagaaaa atagtggcca aagtctggtc agtgatgaat tagttttggg ataaagaaaa
     9841 aggctgagat ctggtgtctc agtcttttat ttcttgcgtt ttgcaaaatc tacaaatcta
     9901 aatttctcta acttatggcg actttcggta aactgaaatt ggcgtccgtc ttggagatag
     9961 acttttgatt taatcgaaac gacataaggg tctttgccaa tgtccatgag aatcttgtct
    10021 cggtcgcttg agtggtcaat ggtgatttcc ttctgagcat aatcaataag gagtttgagg
    10081 ccattttcta tataagaata gatagactgc tcagcaattt ggcgagtgag atttggaatt
    10141 agttccatat ccagatagtc cgtatccaga acggatacca gatcatccac cacacgctgg
    10201 cggaccacct tccaaaccat ccgaaactct gggaaaccgg ttatcagtga ggatttttta
    10261 tcaataataa tcttgtccag actgaccacg ttggttttag agcgcagtcc aagttcttta
    10321 actagttctt ggtagctggt taggttggat acagggaaat tgacggtttc ttctttgacg
    10381 acttgagaac cttgccctct tatctttttg atcaatcctt cctcttggag gagagacagg
    10441 gcctttcgga cggtatcacg actcacttga tattgctcca taaggtcgtg ctcgctagga
    10501 aggaaatctc cgacagcgta agtctcgttt tgaatggttt cttggatttg cttaaataat
    10561 tgttggtatt tcttcatttt gcctgcttaa ataggttttt agtctagttt aatgaacacc
    10621 tgtatctatt ttaccataca agtgctagaa aatttacaaa aaaacaccct ttttatacca
    10681 aacaagttgc agacaagttt ggtatcgttt acaatatact tatcaaatca aacttgcttt
    10741 gacaagtata aggagaatca aatgggaaaa tttgaacaag aagccaaaga tctgcttcag
    10801 gcaatcggag gcaaagaaaa tgtgactgcc gtaactcact gtgcgacacg gatgcggttt
    10861 gttttaggag atgataagaa ggctaatgtt aaagctatcg agtcaattcc agctgttaaa
    10921 ggaaccttta caaatgcagg tcaatttcag gtaatcattg gaaatgacgt gcccatcttt
    10981 tataatgatt ttacagccgt ttcaggtatt gagggtgttt ccaaagaagc agccaagtct
    11041 gcagctaaga gtaatcaaaa cgtggtccaa cgtgttatga ccactctggc ggaaatcttt
    11101 actccgatta ttccagcctt gattgttggt ggattgatcc tcggtttccg taatgtcttg
    11161 gaaggtgtcc attggtcgat gttggatggc aagaccatca cagaatcctc tcagttttgg
    11221 gcaggggtta atcacttcct ctggttgcct ggtgaagcta tcttccagtt cttaccagta
    11281 gggattactt ggtctgtttc tcgtaagatg ggaaccagcc aaattttggg aattgttctc
    11341 ggaatctgtt tggtatcgcc tcagttgctc aatgcctatg cggttgcttc aacgccagca
    11401 gctgatatcg cggcaaactg ggtttggaat tttggctatt ttactgttaa tcgtatcggt
    11461 taccaagccc aagttatccc agccttgctt gcaggtttga gtctgtctta tcttgaaatc
    11521 ttctggcgca agcatatccc agaagtcatt tctatgattt ttgtaccttt cttgtcattg
    11581 attccagcct tgattttggc tcatactgtt ttgggaccaa tcggttggac aattggacaa
    11641 ggactttcat cagttgtctt ggcaggttta actggtccag ttaaatggct cttcggtgca
    11701 atttttggtg ccctctacgc tccatttgtc atcacaggtc tgcaccatat gaccaatgcc
    11761 attgatacac aattgattgc ggatgctggt ggcactgccc tatggccaat gattgctctt
    11821 tctaatattg ctcaaggctc agccgtgttt gcctattatt tcatgcatcg ccatgatgag
    11881 cgtgaggctc aggtttcact tcctgcaacc atttcagcct atctcggtgt tacagaacca
    11941 gctctttttg gggttaacgt aaaatatatt tatccatttg ttgctgggat gactggttca
    12001 gcccttgcag gcatgttatc cgttactttt aatgtaactg cggcttctat tggtatcggt
    12061 ggtttgccag gtattctctc tattcaacct caatacatgc tgccatttgc aggaactatg
    12121 ctagttgcga ttgttgttcc aatgctcttg actttcttct tccgcaaggc tggtctcttt
    12181 acaaaaacag agggcgatac gaacttgcag gcagaattcg ttgctcaaga agaagcagaa
    12241 tttgtgaacc atgaaccagt agaacttact tcggtagaaa ttatcagccc actaactggc
    12301 caagtgaaag aattgagtca agcgacgaat cctatttttg catcaggtgt catggggcaa
    12361 ggtctggtca ttgaaccaag ccaaggtgag ttgacctctc cagttaatgg gacagtgacg
    12421 gttcttttcc ctaccaagca tgccatcggc attgtctctg acgagggagt tgaattgctc
    12481 atccacatcg gtatggatac agtaggtctt gatggcaaag gttttgaaag tcttgtagtc
    12541 caaggagatc acgttacagt tggtcagcaa ctgattcgtt ttgatatgga tgtcattaag
    12601 gctgcaggtc tggtgacaga aactcctgtt atcatcacca accaagatgc ttatacagcg
    12661 actattcctg gaacttatcc gacaacgatc caagctggag catctctcat ggtcgctaca
    12721 cgaatctaat actcttcgaa aatctcttca aaccacgtca gctctatctg caacctcaaa
    12781 atagtgtttt gagcaacctg tggctagctt cctagtttgt tctttgattt tcattgagta
    12841 taattgaaat agcacaaata gggacggaag actgcttcct cccttttatc agaaaaggag
    12901 aaacagatga cacttgataa aggaaaagta gtctatcaaa tctatccaaa atcttacaaa
    12961 gacaccactg aaaacggtat tggggatttc cgtgggatta tcgaaaaaat tccctattta
    13021 gccaaactag gtgtggatat ggtctggctc aatccattct atccaagccc tcaacgggat
    13081 aatggttacg atatttcaga ttatatggca gtggatcctc tttttggtga tatggctgat
    13141 tttgaggaaa tggtgtgtgt cggtaaagag cacaagattg actttatgct ggacatggta
    13201 cttaatcatt gttcgacaga gcatgaatgg tttcagaaag ccctagctgg tgataagtat
    13261 tatcaagact ttttcttcat ccaagaccaa ccaacagatt ggcagtctaa gtttggtggt
    13321 tctgcatggg cgcctttcgg ggatactggg aaatactatc ttcacctatt tgatgagacc
    13381 caggctgacc ttaactggcg caattccaat gtccgtaagg agcttttcaa ggttgttaat
    13441 ttctggcgcg acaagggtgt caaaggtttc cgatttgatg tgatcaattt gatcggtaag
    13501 gacgaggttt cagtggactg tcctgaaaat gaagggaagc cagcttatac agacaaaccc
    13561 atcgttcata actatttgcg tatgatgaac caagccactt ttggatctga cgatagcttt
    13621 atgacagttg gggaaatgtc ttctaccacc atggaaaact gtgtcctcta ttcgtcgcct
    13681 gatcgtcagg aattatccat gacctttaat tttcatcacc tcaaggtgga ctataaggat
    13741 ggacaaaagt ggaccttggc tccctttgat tttgaagagt tgaagagtct ttaccatagt
    13801 tggggtaagg aaatgagtga taaagacggt tggagtgccc tcttttggaa caatcacgat
    13861 caaccacgtg ccttgaaccg ttttgtcgat attcaaaact tccgcaagga aggagccacc
    13921 atgctagcag ccagcattca cctgtcacgt gggacacctt atatctacat gggtgaggaa
    13981 atcgggatga ttgacccaga ctatgattct atgactgatt atgtggatgt cgaatcgctt
    14041 aatgcctatc agatgctctt agaagaagga aagagtcagc aagaagcctt ccagattatt
    14101 caagctaagt cgcgtgataa ttcacgtatt cccatgcagt gggatacttc tgaaaatgca
    14161 ggattttcaa caggcactcc atggttgaaa gttggtaaat cctataaaga tatcaatgta
    14221 gaaaatgaaa tcaaaggtct gattttcacc ttctatcaag acttgattcg actgcgtaag
    14281 gaaatgccaa tcatttcaga aggaaattac aaaccagcct ttgaagatag caaacaagtc
    14341 tacgcttttg aacgccagtt tgaggatcaa aagttactag tgctcaataa cttttatgcc
    14401 aaagaagtgg aaatcgactt gccagcagtc taccaaaatg gacagatttt gatttcaaac
    14461 tatgaagatg cagaagtgtc tgagaaaatt ctactcaaac cctatcaaac actggctatt
    14521 tatgtaaatt aatactcttc gaaaatctct tcaaaccgtg tcagttttat ctgtaacctc
    14581 aaagcggtgc tttgagcaac ctgtggctag cttcctagtt tgctctttga ttttcattga
    14641 gtataaactt agcaaggaga cctctttttc agaaaagcag ggttcttttt gctgtctatt
    14701 ccatccttga aagtgtagaa caaactggtc agggtggcta gtttgtggta taatgaaagg
    14761 gactcaaaaa gaaacaggag aaaaatgatg gatttacttt tagcaattgt attgattgtg
    14821 ctagcttttc taggaggagc tcttggagga atgtacttgg ttcgtaagca aatcgaaaaa
    14881 gaatttgctg acaacccacg tttgaatgct gaagcagttc gtactctttt gagtgcaaat
    14941 ggtcaaaaac caagcgaagc taaggtacaa caagtttacc accaaatcat ccgccaacaa
    15001 aaggcagccc ttgctaacaa taaaaagaaa aaataaataa ggaaaagtct gggatgaaag
    15061 ttccagactt ctcactatgt tggttatggt ttagggatga gactttttcc ttgcattcta
    15121 ttgatatttg attatgatta agagagaggt agttgacatt ctatcaagct gtcggtcagt
    15181 tcgttcagta caaggaatca taatgaacga tcaatcagaa aaaagactag aaagaagact
    15241 gtatggataa tcgaccaatt ggttttttgg attcgggtgt cgggggcttg accgttgtgc
    15301 gcgagctcat gcgccagctt ccccatgaag aaatcgtcta tattggagat tcggcgcggg
    15361 cgccctatgg cccccgtcct gctgagcaaa ttcgtgaata tacttggcag ctggtcaact
    15421 ttctcttgac caaggatgtc aaaatgattg tcattgcttg taacactgcg actgcggtcg
    15481 tctgggaaga aatcaaggct caactagata ttcctgtctt gggtgtaatt ttgccaggag
    15541 cttcggcagc catcaagtcc agtcaaggtg ggaaaatagg agtgattgga acgcccatga
    15601 cggtacaatc agacatatac cgtcagaaaa tccatgatct ggatcccgac ttacaggtgg
    15661 agagcttggc ctgtcccaag tttgctccct tggttgagtc aggtgccctg tcaaccagtg
    15721 ttaccaagaa ggtggtctat gaaaccctgc gtcccttggt tggaaaggtg gatagcctga
    15781 ttttgggctg tactcattat ccactccttc gccctattat ccaaaatgtg atggggccaa
    15841 aggttcagct catcgatagt ggggcagagt gcgtacggga tatctcagtc ttactcaatt
    15901 attttgaaat caatcgtggt cgcgatgctg gaccactcca tcaccgtttt tacacaacag
    15961 ccagtagcca aagttttgca caaattggtg aagaatggct ggaaaaagag attcatgtgg
    16021 agcatgtaga attatgacaa ataaaattta tgaatataag gatgaccggg actggtatgt
    16081 tgggtcttat agtatttttg gtggcgttaa cagtttgagc gactataaga cagattttcc
    16141 tctgtttgaa ttctccaaaa tatttggaga tgaagagtat ggtttcccgc tttcagttac
    16201 tgttttacgc tatggttcta cctaccgttt gttctccttt gtggtagaca tgcttaatca
    16261 agaaatggga cgaaacttgg aagttattca acgtcatggg gccctgctct tggttgaaaa
    16321 tgggcaactc ttgtatgtag aattgcctaa agaaggggtc aatgttcatg atttctttga
    16381 gacaagcaag gtcagagaaa ccttgttgat tgcgactcgt aacgaaggta aaaccaagga
    16441 attccgagct atctttgata agttaggcta cgatgtggaa aatcttaatg actaccctga
    16501 cctgcctgaa gtagcagaaa caggtatgac ctttgaagaa aatgcccgcc ttaaggcaga
    16561 aaccatttct caattaacgg gcaagatggt tttggcagat gattctggtc tcaaagtcga
    16621 tgtccttggt ggcttaccag gcgtctggtc agctcgtttc gcaggtgtgg gagcaactga
    16681 ccgtgaaaat aatgccaaac tcttgcacga attggccatg gtctttgaac tcaaggaccg
    16741 ctcggctcag ttccacacaa ccctagtcgt agccagccca aataaggaaa gtttagttgt
    16801 tgaagcagac tggtcaggtt atattaactt tgaacctaag ggtgaaaatg gctttggcta
    16861 tgatcccctc ttccttgtag gagagacagg tgagtcatca gctgaattaa ccctggaaga
    16921 aaaaaatagt caatctcacc gtgccttagc cgttaagaaa cttttggagg tatttccatc
    16981 atggcaaagc aaaccatcat tgtaatgagc gattcccatg gcgatagctt gattgtggaa
    17041 gaagtccgtg atcgctatgt gggcaaagtc gatgccgttt ttcataacgg cgattctgaa
    17101 ctacgtccgg attctccact ttgggagggc atccgcgttg ttaaagggaa catggacttc
    17161 tacgccggct acccagaacg tctggtgact gagcttggtt cgaccaagat tatccaaact
    17221 catggtcact tgtttgacat caatttcaac tttcaaaagt tggactactg ggctcaggag
    17281 gaagaggccg ctatctgcct ctatggtcac ttgcatgtgc caagtgcttg gatggaaggc
    17341 aagatcctct ttctaaatcc aggttctatc agccaaccac gaggtaccat cagagaatgt
    17401 ctctatgctc gtgtggagat tgatgatagt tacttcaaag tggacttttt gacacgagat
    17461 cacgaggtgt atccaggttt gtccaaggag tttagccgat gattgccaag gagtttgaga
    17521 ctttcttgtt ggggcaggag gaaacttttt tgacccctgc taaaaatcta gctgtgttga
    17581 ttgataccca caatgcggat catgcgaccc tcttgctcag tcagatgacc tatacccgtg
    17641 ttcccgttgt gacagatgaa aaacagtttg ttgggacgat tggactcaga gatattatgg
    17701 cctatcagat ggagcatgac ttgagccaag aaatcatggc ggatacggat atcgttcata
    17761 tgacaaaaac ggacgtagcg gttgtttcgc ctgatttcac cattacggag gtcttgcaca
    17821 agctagtaga tgagtccttc ttaccggttg tggatgcaga gggtattttc caagggatta
    17881 ttacgcgcaa gtccatcctc aaggccgtta atgccctctt gcatgacttt agtaaggaat
    17941 atgagattcg atgccaatga gagacaggat ttcagccttt ttagaggaaa agcagggctt
    18001 gtctgtcaat tccaagcagt cctataagta tgatttggag caatttttag acatggtagg
    18061 tgagcggatt tctgagacca gtctcaagat ttaccaagcc cagctagcca atctaaaaat
    18121 cagcgcccag aagcgaaaga tttcggcctg taaccaattt ctatactttc tctatcaaaa
    18181 aggagaggtg gacagctttt accgcttgga attagccaaa caagctgaaa agaagacgga
    18241 aaagccagag attctatacc tagactcttt ttggcaggaa agcgaccatc cagagggccg
    18301 cttgctagcg ctcttaatcc tagaaatggg gcttttgccc agtgagattt tagccatcaa
    18361 ggttgcggac atcaatctgg attttcaggt gttgcgaatc agcaaggctt cccaacagag
    18421 gattgtcacc attcccacgg ccttgctttc agaattggaa cccttgatgg ggcagaccta
    18481 tctttttgaa agaggagaga aaccctattc tcgtcagtgg gcctttcgtc agttagaatc
    18541 ttttgtcaag gagaaaggtt ttccatcctt atcagctcaa gtcttacgtg aacagtttat
    18601 tctaagacaa atagaaaaca aggtcgattt gtacgaaatt gcaaaaaaat taggattaaa
    18661 aacagtcctg accttagaaa aatatagata atggatatta aattaaaaga ttttgaagga
    18721 cccctggact tgctcttgca tctggtttct aagtaccaga tggatatcta cgatgtgccc
    18781 attacggaag tcatcgaaca gtatctagcc tatgtctcaa ccctgcaggc catgcgtctg
    18841 gaagtgacgg gtgagtacat ggtcatggct agtcagctca tgctgattaa gagtcgtaaa
    18901 ctccttccga aggtagcaga agtgacagac ttgggggatg acctggagca ggacctcctc
    18961 tctcaaatcg aagaatatcg caagttcaag ctcttgggtg agcacttgga agccaagcac
    19021 caagaacggg cccagtatta ttccaaagcg ccgacagagt tgatttacga agatgcggag
    19081 cttgtgcatg acaagacgac cattgacctc tttttggctt tttcaaatat cctagccaag
    19141 aaaaaagagg agtttgcaca aaatcacacg acgatcttgc gggatgagta taagattgag
    19201 gacatgatga ttatcgtgaa agagtccttg attggacgag atcaattgcg cttgcaggat
    19261 ttgttcaagg aagcccagaa tgtccaagag gtcatcaccc tctttttggc aaccctagag
    19321 ttaatcaaaa cccaggagct gatcctcgtg caagaggaga gtttcggaga tatctatctc
    19381 atggaaaaga aggaagaaag tcaagtgcct caaagctaga cttgatagag aggaaagatg
    19441 agtactttag caaaaataga agcgctcttg tttgtagcgg gtgaagatgg gattcgggtc
    19501 cgccagttag ctgaactcct ctctctgcca ccgacaggca tccagcaaag tttaggaaaa
    19561 ttagcccaga agtatgaaaa ggacccagat tccagtttgg ctttgattga gacaagtggt
    19621 gcttatagat tggtgaccaa gcctcaattt gcagagattt tgaaggaata ctctaaggcg
    19681 cctatcaacc agagcttgtc tcgggctgcc cttgagacct tgtccattat tgcctacaaa
    19741 cagccgatta cgcggataga aattgatgcc atccgtggag ttaactcgag tggagccttg
    19801 gcaaagttgc aggcttttga cctgataaag gaagacggga aaaaggaagt attggggcgc
    19861 cccaacctct atgtgactac ggattatttc ctagattaca tggggataaa ccatttagaa
    19921 gaattaccag tgattgatga gcttgagatt caagcccaag aaagccaatt atttggtgaa
    19981 aggatagaag aagatgagaa tcaataagta tattgcccac gcaggtgtgg ccagtaggag
    20041 aaaagcagaa gagctgatta agcaaggctt ggtgacggtt aacggccaag tggtgcgtga
    20101 actagcaacc actatcaagt caggcgacaa ggtcgaagtt gaaggtcaac ctatctacaa
    20161 cgaagaaaag gtctactatc tgcttaacaa accacgcggt gtgatttcca gtgtgacaga
    20221 tgataagggt cgcaagacgg ttgtcgacct cttgcccaat gtcaaagagc gtatttaccc
    20281 tgtgggtcgt ttggactggg atacatcagg tgtcttgatt ttgaccaatg atggggactt
    20341 tacagacgag atgattcacc ctcgtaatga gattgacaag gtttatgtcg cgcgtgttaa
    20401 aggtgtggtc aataaggaca atctccgccc cttgacccgt ggtcttgaga ttgatggtaa
    20461 gaaaaccaag ccagctgttt atgaaattct caaagtggac ccagtcaaaa atcgctctgt
    20521 ggtgcagttg accatccatg aagggcgtaa ccatcaggtt aaaaagatgt ttgaagctgt
    20581 tggtctccaa gtagataagt tgtctcggac tcgtttcgga cacctagact tgacaggact
    20641 ccgtccagga gaatcccgtc gtcttaataa aaaagaaatc agccaactac acaccatggc
    20701 tgtaactaag aaataatgaa acgaatttta atagcgcctg tgcgctttta ccaacgtttt
    20761 atctcaccag tctttccacc ctcttgtcgc tttgagctga cttgttccaa ctacatgatt
    20821 caggctattg aaaaacatgg ttttaagggg gtattgatgg gcttggctcg gattttacgt
    20881 tgtcatccct ggtcgaaaac aggtaaggac cccgttccag accgcttttc ccttaaacga
    20941 aatcaagaag gggaatgagg tggggtaaat agatttcaaa atgataaaaa cgcatcctat
    21001 caggtttgag tgaacttgat aggatgcgtt ttagaatgtc aaaattttat actcttcgaa
    21061 aatctcttca aaccgcgtca gctttcatct gcaacctcaa aacagtgttt tgagcaacct
    21121 gcggctagtt ttctagtttg ctctttgatt ttcattgagt attaaattga gtttgaagtg
    21181 gcttatttca aagctttttg tatgtcttca atcatgagtt ttgttgattc aagtccgcct
    21241 ccgcttagat accagaggtc tggtgttagt tggataatct taccattttt agcagcaggt
    21301 gtttcagcga taagggcatt ttctaggaca ccgtcgttgc tagagttgtc cccaccgatg
    21361 gcaagggtac ggttgatgac aaagaggatg tcagggttga tttctttgac actttcaaag
    21421 ctgacttctt gtccgtggcg tgagtcttca aattttgtat cagttggttt gaatttcaag
    21481 gtttggtaca agaaagagaa acgagatttg gcaccaaagg ctgccatttt tccttcatta
    21541 aggaggatcg caagggcttt tttgtcagag ctttcatttt tagtagcgac ttcttggatg
    21601 ctcttgtcta gcttggccaa ttcttccttg gctttctgtg taccagtttc gccgaaggca
    21661 cttgctaagg attcgatatt agccttggta gaagtccagt agtcgtcctt gcttgcttgg
    21721 aagagaacgg ttggggcgat ttctttgaat ttgtctacga atttttgtgt acgtggtgaa
    21781 gcgataatca aatcaggctc aagggcggcg atagcttcta aatcaggttc tttcatagaa
    21841 ccaacatttt tgacagttcc cactaggtct tttagataag tcggaacagt ttttgtaggc
    21901 attccgacga tatttttttc aaatcctaaa gcgcgaatag tatccgcagc gccgaggtca
    21961 aaggtcacaa tcttttcagg aactttggaa agtttgacct cgtccagtga acttttaatg
    22021 gttacctctg ttggagcaga gctactggtc tctgtctgac tagtgcttga gtttgtacta
    22081 catgcaccaa gtaggagcaa gaagctggcc actagggcag tgaaataaag tttaagggat
    22141 gttttcataa tttctccttt ttaaaatgtg ataacgattt agggagtctc ttaatcttat
    22201 tgactaagag actgaaggtt ctctaacttg agcttttatg ttactagcta tagatacaga
    22261 tctttttgtt attgatatca gctagcgtga tgggaatctc ataaagttga ctgagcaggt
    22321 cagcctgcat gatttgatcg gttcttccct tgctaaagac ctggccgtcc ttgaaggcga
    22381 caatttcatc tgcatactga ctggccatgt tgatatcgtg gaggacgatg ataatggtct
    22441 tgccgagttc ctccaccagt cgtcgaagaa tctgcatcat gctgacgctt tgcttgatat
    22501 cgagattgtt gagtggttcg tccagcaaga taaagtccgt atcctgggcc agtaccatag
    22561 cgataaagac gcgctggagt tgcccccctg acaggctatt gatgtagcgg tcttttaagt
    22621 tggtcagttc caaatagttc agagtttctc ggattttttc ccagtcttct gatctaagtc
    22681 gacctcggct gtagggaaaa cgtccaaaac tgaccagttc ttcaacagtc aatttggctt
    22741 ggtaattgat tttctgtttt aggatggtta gttcttgagc cagttcttgc gaattccagc
    22801 tctcgatttc acgtcctttg atactgagaa ctccctgatc tttcttggtt agtctgctca
    22861 tgatggagag gagagtcgat tttccagcac catttggacc aataaaggct gtcagttttt
    22921 gaggactgac ttcaagcgaa atgccttgca aaatatcctg tttttgaatg gatttgtcaa
    22981 tgttttccag tttcactgac gagacctcct atatagtaag ataaagaata agaagccacc
    23041 cacactctca atgatcatac tgatacgaat ttccagtgca aagactcgtt caatcaaggc
    23101 ttgccccaag gttaagctaa taaatccaac cagaatggcc actataaaga gtaacttgtg
    23161 ctgatagtct ttgacaatca ggtaggtgag gttggccagc ataaagccga agaaggccat
    23221 aggtcctacc aaggcagtgg ccgttgaggt caaaagcacg attccccaga ggagctcttt
    23281 ctgttctttt tcaacatcga gtcccaatat ctgagccgtt tctctttgca ggtgcaagac
    23341 atctagaacg actgcttttc gaaagaaaaa gattgtcaaa gcgaggatga tcagagaacc
    23401 gatggctagg atggaagtgt taagatgttg aaaggaggca aaaagactat tttgcagttt
    23461 atcgtattcg tttggatcca ttaggacttg aaggaaggtg ctgatatttc gaaagagact
    23521 tctgagcgct agacagatca gcaggatgaa gaccaggtct tgcttcatca gtgtcttcaa
    23581 gtaaccttgt aaggcgagaa agaagaagga ctggacaaga agtaagacta ggaattctaa
    23641 gataggggat ttgccaagtt gaagaaactt gctttcaaaa accagtagta gggtttgtag
    23701 taggacgtag aaggattcaa ttcccaaaat actaggcgtc aggaagcgat tttccgtcag
    23761 ggtttgaaaa ctaatggtcg aaatcccagt cgcgatggct accaagagat aaacgatgat
    23821 cttttgggaa cgcaacttcc aagcaaaggc tgacaagtga gtgatgggcc aaaagtagag
    23881 aagacaagct ccgatggcaa gaataatgag aatccagaag agcttggtat gtttgctttt
    23941 agtctgcatc ttttcgtccc cctctccaga gaagtaggat aaagacgaga ctaccgatga
    24001 ttcctagcaa gagactgaca gacaactcat agggcctaat cagaactcgg gataggatat
    24061 cgcaagccag aactagattg gcaccaacca gtgcgaccat gagtttggtt tgacttagat
    24121 tatctccata gcgcttgcga acaagattgg gaacgataac tccgagaaat ggtaagccac
    24181 ccacggtaat catggtgacg cttgtcgtta gcgccaccag aaagagggcc agtttttcaa
    24241 gtagggagta ggaaatcccc aaactctcgc tggtttcttt ccccagattc atgatggtga
    24301 aggtttggga taatttccaa acggttatca ggatgatgag gcctaagaag agccactcat
    24361 actgatgggt ctgaatcatg gagaaggagc cctgggtcca ggcagtcata ctctgaacca
    24421 gattgaaacg ataggcgata acttctgtga ctgagccgat aatcccgcta tagatgatcc
    24481 caatcagagg caacatccac ctttccttta cagtaaaaat ggtcataaag gctaggaaga
    24541 agagggtgaa tacgatggat gaaacaaaag cgaagagcat cttttgggtc agactagccg
    24601 atggaaagac aaaaaggctc agcaccattc ccagtttggc ggcttcagtc gttccaactg
    24661 tactcggtgc agcaaactga ttttgggtaa tagtctgcat gagaaggcct gccatactca
    24721 tactagaggc agtcaggaga atactgatag ttcttgggag acgggactct tgaaagagga
    24781 gccaggtctg ctggtcgaaa tcaaatagct ttccccatga aaaatcactg gtcccaatgc
    24841 taatagagag aaagactagg agtagaagta agccaattaa ataatgagaa agtttcatac
    24901 cccgtccttt catgtagatt tggtatcgaa agatatctgc ggatataaat gtaacattat
    24961 ttttctaatc tgtcaataaa atttctgata atttaataaa tacaacaagg agagagcaac
    25021 aagactttct cctttgttat cctattctaa aatgttttta ccttaatctg ataaaataat
    25081 atcttcgagg gagtagctag ccgtccaatc aagatattgt ttagcttttg aagcatctgc
    25141 taggacactg gctgggtcac tagcacgtcg agcaacaatc tcgtgtggga ttttttaatt
    25201 tagtaattct tcagcagttt taaagatttc tttgatagta tagccttttt tagttcctaa
    25261 gttaaagatt tgagaagaac tgtcttcttg aaataggtag ttcattcctt taacatgagc
    25321 ctatgcaagg tccaagacat aaatgtaatc tcgaatacat gaaccgtcac gtgtatcgta
    25381 gtcatctcca aatattttta agctatcatt ttgtcccaat gcggtcttgt tgatatttgg
    25441 aatgatgtga gttggatttt tcacacgcag accgtttgaa gcatccattt cagccccagc
    25501 agcattaaag taacggaaaa taacatattt ccagtcgtag cgattggcca tccagtaaat
    25561 cattcgttcg cccatcagtt ttgtctctgc ataagggttg acagggtcga gcagggtatc
    25621 ttcagtcacc ggcttgtcaa tacagttatt tccatagaga gaagcagtcg aagagaacat
    25681 gattttttga atgccaactt cagataagac tttgagaact tggttcatac cagcaacgtt
    25741 ggcagtgaag tatttacttg gattttcaat actttcgctc acaacaattt cacctgcaca
    25801 atggaggaca gcatcaatct gattttcttc taagtaagcc tttaaggcgc tagcatcata
    25861 aacatccagt tctttaaagc tagcacgact atccacagcc gctcgatttc ctgtggagag
    25921 attatcgagg acatgcacct gatagccagc atttaaaaga gctttaacgg tatgggagct
    25981 aatgtagcta gtcccacctg taacaagaat tgttttggtc atgatttttt ccttttctag
    26041 ttttgtacta tagtaaattg aaataagata tgaacaact
//
LOCUS       DAVLXF010000022        24682 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLXF010000022 DAVLXF010000000
VERSION     DAVLXF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.022"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(72..269)
                     /gene="blpI"
                     /locus_tag="VYO12_000846"
     CDS             complement(72..269)
                     /gene="blpI"
                     /locus_tag="VYO12_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEV1587486.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            550..2703
                     /gene="blpA"
                     /locus_tag="VYO12_000847"
     CDS             550..2703
                     /gene="blpA"
                     /locus_tag="VYO12_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HEV1587487.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLVIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILKACEVAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2714..4075
                     /locus_tag="VYO12_000848"
     CDS             2714..4075
                     /locus_tag="VYO12_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1587488.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSIISSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTSNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPIITDTREVLITYYVASDYLPLLDKGQTVRLKLEKIGNHGITIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            4132..4287
                     /gene="blpC"
                     /locus_tag="VYO12_000849"
     CDS             4132..4287
                     /gene="blpC"
                     /locus_tag="VYO12_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1587489.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDILYSLNIIKHNNTKG
                     LHHPIQL"
     gene            complement(4332..5672)
                     /locus_tag="VYO12_000850"
     CDS             complement(4332..5672)
                     /locus_tag="VYO12_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1587490.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGIGLTFERIFKAFLLKILL
                     AFVFVMIGYMVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQEQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRREILDTGFQK
                     SLTKINWIMGAYYLVIQSLSYFEYEQGIQSTTVRHLILVFYLLFFMGVIKKLDTYLKD
                     KLHDRLDQEQVLRYRDIERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYDLVLRDSSQKLQDNKYDLGRLVNVRDRALKSLLAGKFIKAREKDIVFNVEVPEEI
                     QVEGMRLLDFLTIVSILCDNAIEASAEAGQPHVSIAFLKSGAQETFIIENSIKEEGID
                     ISEIFSFGVSSKGEERGVGLYTVMKIVESYPNASLNTTCQDQVFRQVLTMIPTE"
     gene            complement(5686..6423)
                     /locus_tag="VYO12_000851"
     CDS             complement(5686..6423)
                     /locus_tag="VYO12_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1587491.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIREQDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(6428..6766)
                     /locus_tag="VYO12_000852"
     CDS             complement(6428..6766)
                     /locus_tag="VYO12_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1587492.1"
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEKASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7042..7371
                     /locus_tag="VYO12_000853"
     CDS             7042..7371
                     /locus_tag="VYO12_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1587493.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7539..8588)
                     /locus_tag="VYO12_000854"
     CDS             complement(7539..8588)
                     /locus_tag="VYO12_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587494.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8585..9316)
                     /locus_tag="VYO12_000855"
     CDS             complement(8585..9316)
                     /locus_tag="VYO12_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587495.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9384..9794
                     /locus_tag="VYO12_000856"
     CDS             9384..9794
                     /locus_tag="VYO12_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1587496.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9804..10091
                     /locus_tag="VYO12_000857"
     CDS             9804..10091
                     /locus_tag="VYO12_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587497.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(10457..11593)
                     /gene="dnaJ"
                     /locus_tag="VYO12_000858"
     CDS             complement(10457..11593)
                     /gene="dnaJ"
                     /locus_tag="VYO12_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1587498.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(11798..12151)
                     /locus_tag="VYO12_000859"
     CDS             complement(11798..12151)
                     /locus_tag="VYO12_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587499.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDYTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(12164..13987)
                     /gene="dnaK"
                     /locus_tag="VYO12_000860"
     CDS             complement(12164..13987)
                     /gene="dnaK"
                     /locus_tag="VYO12_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1587500.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14467..14991)
                     /gene="grpE"
                     /locus_tag="VYO12_000861"
     CDS             complement(14467..14991)
                     /gene="grpE"
                     /locus_tag="VYO12_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1587501.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15018..16052)
                     /gene="hrcA"
                     /locus_tag="VYO12_000862"
     CDS             complement(15018..16052)
                     /gene="hrcA"
                     /locus_tag="VYO12_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1587502.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRIADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16216..16725)
                     /locus_tag="VYO12_000863"
     CDS             complement(16216..16725)
                     /locus_tag="VYO12_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1587503.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            17332..19665
                     /locus_tag="VYO12_000864"
     CDS             17332..19665
                     /locus_tag="VYO12_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1587504.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAMVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDKEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            19678..21141
                     /locus_tag="VYO12_000865"
     CDS             19678..21141
                     /locus_tag="VYO12_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1587505.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTNDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21141..22709
                     /locus_tag="VYO12_000866"
     CDS             21141..22709
                     /locus_tag="VYO12_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1587506.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            22766..23563
                     /gene="psrA"
                     /locus_tag="VYO12_000867"
     CDS             22766..23563
                     /gene="psrA"
                     /locus_tag="VYO12_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1587507.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            23574..24176
                     /locus_tag="VYO12_000868"
     CDS             23574..24176
                     /locus_tag="VYO12_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1587508.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSINTGLSYKKGD
                     LSINKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIG
                     KFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQAL
                     YNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(24124..>24682)
                     /locus_tag="VYO12_000869"
     CDS             complement(24124..>24682)
                     /locus_tag="VYO12_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1587509.1"
                     /translation="NKDETTSYPIYEIPKAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
BASE COUNT         7944 a         4826 c         4512 g         7400 t
ORIGIN      
        1 atattttgta agtaagtaaa taagacagta aattaatagc gataataata ctatatttaa
       61 gaatcataat cttacaaaga ggacataatt cctgaaccta cacaaataag tgttgctgct
      121 cccccagtta tcggaccagt cgcagcagct aatagtactg ctccaataca accaccgatt
      181 gcagatccta aattgcctct tcctccacta actatttcga gttcttcatt atccataaca
      241 gaaaattgtt ccatcatttt tgtattcatg acaaatactc cttttttatt tttaattttt
      301 gtcttgttgc aactttgaca agtttagtat atcattattt tttaattttt ttcatccaaa
      361 tcttgaattg ccatcgaaac gtcttgaatt agctttttta tttcaagcca cctctaaatg
      421 tttaaaaaaa ataatttcta atcacttttt taccattcag gaagttttaa tgactattca
      481 acatttcata aaatatgaac ttagttttat ggcataatat acctatccac tatatgaaag
      541 gaattgccaa tgacttctta taaacgtaca tttgttcctc aaatagatgc gagagactgt
      601 ggtgtcgctg ccttagcctc gattgctaaa ttctatggtt cagatttttc tctagctcac
      661 ttgagagaac ttgcaaagac caataaagaa gggacgactg ctcttggcat tgtaaaagcc
      721 gctgatgaaa tgggctttga aacaaggcct gttcaagcag ataaaactct ctttgacatg
      781 agtgatgtcc cctatccatt tatcgttcac gttaacaaag aaggaaaact ccaacattac
      841 tatgttgtct atcaaacaaa gaaagactat ctggttattg gtgatcctga cccttctgta
      901 aaaatcacta aaatgtcaaa agaacgcttt ttctctgaat ggactggagt agctattttt
      961 ctagctccca aacccagcta tcaaccccat aaagataaaa agaatggtct actaagcttc
     1021 cttcctctga ttttcaaaca aaaatctctc attgcttaca ttgttctctc aagcttattg
     1081 gtcactatta tcaatatagg tggttcttat tatctccaag gaatcttaga tgaatacatc
     1141 ccaaatcaga tgaaatcaac tttaggaatc atctcagttg gtctggttat cacctatatc
     1201 ctccaacaag tcatgagctt ctccagagat tatctcctaa ccgttctgag ccagagatta
     1261 agcattgatg tgattttatc ctatattcgc catatttttg aacttcccat gtctttcttt
     1321 gcgacacgtc gtacaggaga aatcatttca cggttcacag atgctaactc tattatagat
     1381 gccttggctt ctaccattct ttctcttttc ctggatgttt ctattctgat tcttgtaggg
     1441 ggcgtcttac tggcacaaaa ccctaatctc ttccttcttt ctcttatttc tattcctata
     1501 tacatgttca tcatcttttc ttttatgaag cctttcgaaa aaatgaacca tgatgtcatg
     1561 caaagtaatt ctatggttag ctctgccatt atcgaagata tcaacgggat tgaaactata
     1621 aagtcgctca cgagtgaaga aaatcgctat caaaatatag acagcgaatt tgtagattat
     1681 ttggaaaaat cctttaagct cagtaaatat tctattttac aaacaagttt aaagcaggga
     1741 acaaaattag ttctgaatat ccttatccta tggtttggcg ctcaattagt catgtcgagt
     1801 aaaatttcta tcggtcagct gattaccttt aacacacttt tttcttactt tacaactcct
     1861 atggaaaata ttatcaacct ccaaaccaaa ctccaatctg cgaaggtcgc taataaccgt
     1921 ttgaacgaag tctatctagt cgaatctgaa tttcaagttc aagaaaaccc tgttcattca
     1981 cattttttga tgggcgatat tgaatttgat gacctttctt ataagtatgg ttttggacga
     2041 gataccttaa cagatattaa tctcacgatt aaacaaggag ataaggttag cctagttgga
     2101 gttagtggtt ctggtaaaac aactttagcc aaaatgattg tcaatttctt tgaaccctac
     2161 aaagggcata tttccatcaa tcatcaggat attaaaaaca ttgataaaaa agtcttgcgc
     2221 cgtcatatta attacctacc ccaacaagcc tatatcttta atggctctat tttggaaaac
     2281 ttaaccttgg gcggtaatca tatgattagt caagaagata ttctaaaagc ttgtgaagta
     2341 gctgaaatcc gtcaagacat tgaaagaatg cctatgggct atcaaactca gctctctgat
     2401 ggagctggtc tatcaggagg acagaagcaa cgaatcgctc tcgctcgtgc tcttttaact
     2461 aaatctcctg ttttaatact agatgaagct actagcggtc ttgatgtctt gactgagaaa
     2521 aaagttatag ataatcttat gtccctaact gataaaacca ttctctttgt agcccatcgt
     2581 ctcagtatag ccgaacgaac caaccgtgtc attgttcttg accaggggaa aatcattgaa
     2641 gttggtagcc accaagagtt aatgcaggcg caaggtttct accatcatct attcaataaa
     2701 taaggagaat gtcatgaatc ctaatctttt tagaagcgtc gagttttatc agagacgtta
     2761 ccataactat gcgacagtgt taattatacc tctttcatta ctatttactt tcatcttgat
     2821 tttctccctt gttgccacaa aagaaattac tgtcacttcc caaggagaaa tcgcccctac
     2881 aagtatcatt tcatctattc agtcaaccag tgataatcct atccttgcta atcatttagt
     2941 ggcaaatcaa gtagttgaaa aaggggactt actcatcaaa tactctgaaa caatggaaga
     3001 aagtcagaaa actgccttag caactcaatt acaaagactt gagaagcaaa aagaaggact
     3061 tggaattttg aaacaaagct tagaaaaagc gactgatctt ttttctggcg aggatgaatt
     3121 tggctaccat aataccttta tgaattttac taaacaatcc catgatattg aactgggtat
     3181 cacaaagact aacactgagg tttcaaatca agctaatctt gccaatagca gttcatcagc
     3241 tattgaacaa gaaattacaa aagttcaaca acaaattgga gaatatcaag agctgagaga
     3301 tgctatcata aataacagag cacgcttacc aactagcaat ccacaccagt caattttgaa
     3361 tcgttatctt gtagcctcac aaggacaaac acaaggaacg gcagaggagc catttttatc
     3421 tcaaattaat caaagtattg caggtcttga atcatctatc gcaagcctca aaattcagca
     3481 agctggtatc ggaagtgtag caacttatga taatagttta gcaaccaaaa ttgaagtact
     3541 ccgcactcaa tttttacaaa cagcttctca gcaacaacta accgtggaga accaattaac
     3601 agaattaaaa gtacaactag atcaagcgac acagcgctta gaaaacaata ccttaaccgc
     3661 tccaagtaaa ggtatcgttc atctgaacag cgaatttgaa ggtaaaaata gaattccaac
     3721 tggtacagaa attgctcaaa tattccctat catcacagat acaagagaag tactaatcac
     3781 ttactacgta gcttctgact atctacctct actagataaa ggacaaactg taagattaaa
     3841 actggagaag attggaaatc acggcattac catcatcggc caacttcaga caattgatca
     3901 aactcctacc agaacagagc aaggcaatct ctttaaatta accgctcttg caaaactatc
     3961 taatgaggat agtaaactca tccaatatgg cttacaaggt cgcgtcacta gtgtaactgc
     4021 aaagaaaaca tattttgatt atttcaaaga taaaatttta acccattctg attaattttc
     4081 agataacact ctataactat ttattatctt atcaaaaagg agaatcataa catggataag
     4141 aaacaaaact taacttcatt tcaagaacta acaactaccg aactcaacca aattacaggt
     4201 ggaggactgt gggaagatat cttatacagc cttaatataa ttaagcataa caatacgaaa
     4261 ggacttcatc atccaataca actataaaaa ataagaccga gtaacaagaa ctctcggtct
     4321 tatttttttc atcattctgt aggtatcata gtaagcacct gacgaaagac ttgatcttga
     4381 caggtagtat ttaaactggc attgggataa ctttccacaa tcttcataac ggtatagaga
     4441 ccgacacctc tctcctcccc tttagaacta actccaaagg agaagatttc agaaatatcg
     4501 atgccctctt ctttgatgga gttttcaatg ataaaagtct cctgtgctcc actttttaaa
     4561 aaggcaattg aaacatgagg ttgaccggcc tctgcactcg cttcaatagc attatcacaa
     4621 aggatagaca caatggttag aaaatcaagc agtctcatcc cctcaacctg aatctcttca
     4681 ggaacttcga cattaaagac aatgtccttt tctctagctt ttataaattt tccagctagg
     4741 agacttttga gggcacgatc ccgaacattc accaatctgc ccaggtcata tttattgtcc
     4801 tgcaatttct gactggaatc ccttaagacc aagtcataga cctcttttat ctgctccata
     4861 tcctcctctt caatacccag acgtaaactt gtcaagaggt tggtgtagtc atggcgaaaa
     4921 ctccttactt ccttgtaaag ttcctctata tgccgactat agcgttctat atctctatag
     4981 cgcaagacct gctcttggtc cagtctatca tggagtttgt ccttcaaata ggtatccaat
     5041 ttcttgataa cccccataaa aaataggagg taaaacacta gaatgagatg gcgaacagtc
     5101 gttgattgaa taccttgctc atattcaaag taagacagac tttgtatcac caaatagtaa
     5161 gcccccatta tccagttaat cttagtcagg gacttttgaa aacctgtatc tagaatctcc
     5221 cttctcaaac tagtaaaatc atagtccaac catttcaaaa aggctagaga aatgaagaaa
     5281 ttgaaaatta ttatacataa cccagtaaat gagtagccat catatacttg ctcttgtccc
     5341 aaaaatggaa gcacaaaata ggagactcct ctataaaaga gattcaccaa tatcattgga
     5401 aagagaccat aaaagaaaag gagtttttta ggaagccctc ttaacaataa gaaagacaag
     5461 cctatgccgt acaagggttc cataaaataa gataggtaaa catttcctac catatagcca
     5521 atcattacaa aaacaaaggc caacagtatc ttcaaaagaa aggccttaaa aatcctctca
     5581 aaagtgagac caattccatc taccttaaag aaaatgacaa tttttaatcc gtgagtaaca
     5641 agtgtataca acaatatcca agcaacattc ataaactctc ctagctcagt gtaagttatt
     5701 aatagcctca gacacttccc tgaccttata acgcgcgatc agacagcttc caccattggg
     5761 aaagaaaagg agtttttctt tcttatccaa atgcaccaca tttgcaggat tgatgagaaa
     5821 agagcgatgg cactgcaaga gacggggttc ctgcttaaaa acctcctcta aactcgccgt
     5881 aaattctagc ctgtccgtct tggtatagag aataacacga tggggtcttg gggatgtttc
     5941 gagatagtaa acctctttga aaggatattg gaattgggca aattttgatt taaagtaaaa
     6001 gcaatcttcc gccagacttt tactatcttg actattggca tagaggaggg ctgtctcgat
     6061 acgagattca aactcctctg ccgaaagggc cttatcaatg tagtccaaag ctgacacttg
     6121 gtagcgaaag gacagaggca taaactccga gtgagtcgtc acaaagacga ttagggcata
     6181 agggtcttgt tcccgaatct ttctagctac ttccagtccc ttcatctctt catttcgaat
     6241 ctcaatatcc aaaaagaata gctgatgggc ccccttctca tgcacctctg ccagcagttg
     6301 gtccggcttg ccaaatacct caaaagagct aggaatgata tgatgtgctt tcaaaagttt
     6361 ctcaatcgtc gtttcaattc tagtctgttg ggaaaaatca tcttctaaaa caaatattct
     6421 catcttctta ctctccctct ttcaaccatt tttgacgtat ttctctatag cgacgtctgg
     6481 catacttgac agcatattga ccttcttctc cgagaaaaag gattctttct tgaaaatcaa
     6541 tcgattttaa tttatcaaga ttaaccaaac aatttcgatg acatctcatc aaagtttccc
     6601 cacattggtt ctcaagatta cttaaatttt gaagcatatt aaaactagct ttttctgtaa
     6661 caatctgtac ggtatgggct ttagttggat gtgtttggat atagtagata tcgtcgatgt
     6721 ttactttata gactgttttc tgtgtctgaa taatcatatg tttcattgga ttctcctcct
     6781 aagaaaagaa taacagaaaa ggtctagccc aagcacaccc aatcctaaac agtctttgaa
     6841 aactcctaaa aggattattt tctacaacat catgatgagc ttacaactat tttatcataa
     6901 aattcactca gcgcaattca agacatttca atgacaatta aagatttgaa taaaaaaaaa
     6961 gaattaaaag tgataaacta acagtatcaa aacacagttg ccagaagtaa gactggcacc
     7021 cagattaaag gaggcattcc catgacagat acagacccca tcaaaagagc tcatactttg
     7081 attactgact taaacaaagc ctaccaagca tgcaaacagg caagcgctga cgacgtccgc
     7141 tttcaggagc aattaaactc tattcttggt tttctagcca aggctgaaac agtggataat
     7201 cgattcttga ttgaactaga aaaattttac cagacttcca gtcttctcat gggactcagc
     7261 gctcttgatc cagatgctcc aactcgcgcc gcttggcggg cctatgaccg cttccacttt
     7321 gaccaagtca agaccaagtt aatactcaat gagaatcaaa gagcaaacta ggaagctagc
     7381 cgcaagctgt acttgagtac ggtaaggcga cgctgacgtg gtttgaagag attttcgaag
     7441 agtataagtc tctacggacc aaccattatc ctgtagtaac aaaaaagaag ctgagacaga
     7501 ctgaactcaa cttctttttt agtgtcgtat cagcaatgtt agtcctgcat ctgacgtttt
     7561 acctgataag gcaaatacaa gactagtaaa accaaaccag ctcctagtaa ggctagaagg
     7621 gccagttttt cttggaaggt aatcaaccca acaactaatt ccacaagtaa aacaaaactg
     7681 gtcaatcctc gaactacctc ctgtaagcct ttttcctttt gccccttgtc cagcggaaag
     7741 agttgggtca aatactggta gtcaaaggca tgatagaggg ccagcaactg gaagagcaag
     7801 aggtagttaa agagaactac cactgctgtc gcaatccaag cttgctcgat aaaaacctgc
     7861 gccagtacgg aaagtaatag aagacgaaga ctgagagcaa agaggtcgcc atttcgcaga
     7921 taagaacgca gatagagatt ttgccaaatc ttcccaggca ccttctgaac agcctttaag
     7981 ataaagtcca gataggcacg acgcttaacg ctgtttgaaa ttcccttgac ctgcgtaaag
     8041 agggcaaaga aacgaagcaa gacttgctta cgcttgcttt cttgagaaat aacatagtcc
     8101 cagtccagtc cagtttcagt gaaaaatttg ctggcctttt gacgaaagtg gaaatatttt
     8161 cctaccccca ataaaagcac atagagcaga aaaactggca agccataacc cattgctaaa
     8221 aataaaggcg caaataacag caagaaaagg gtctgtacaa agagccaaaa gactagggaa
     8281 atgccagttt gacgcttgag atggagcttg atttcctctt ctccaactaa gagaaagagc
     8341 ttgtctggag cctccatata ggtggcaatt cctccccaaa gtaaaagtaa aacagacgta
     8401 attcctacaa acaaaaggat aggccaatga ttttcaggaa aatgttgtaa gagttgactg
     8461 tactggtagg ctagaaagcc caacaggaca agcaggaaca agacaaagtg gtcattgagc
     8521 acatagcgca gataaccaag acactcctta cgaaaggcct gctttctctt taaaaacaag
     8581 tctttcatag gtcctcctct tttgtcagag ccaagtaaat atcattcaaa ctagcctcag
     8641 gcatatcaaa ggcttcgcgt agttgcagga gattgccttt ggcacgcacc tctcccttgt
     8701 gaagaatgac aaaggcatca cacatcttct ccgccgaatc cagcacgtgg gtactcatga
     8761 gaatagactt gcccttttgc ttctccactt ccaaaagctg aatcaaatca gaaatagcca
     8821 gcggatcaag accaaggaaa ggctcatcca cgatgaaaag acttggatcc accacaaaag
     8881 cacagataat catgaccttc tgcttcatcc cttttgaaaa atgaacaggg aaccagtcta
     8941 atttctgttc caaacggaac atttttaaca agggctctac tcgttcgaaa gccacttttt
     9001 gctcaatacc gtaagccata gcaaccgttt cgatatgctc tctgagggtc aattcctcat
     9061 acagactagg cgtctcagga atgtagccaa tctgcttgcg gtagctagtc gcatctcctt
     9121 gcagagtcag gccattgata ttgatggagc cactataagg tgccaacaga ccgataatct
     9181 cattgatcgt cgttgatttc ccagcaccat tgagaccaat caaaccgacc aactgcccac
     9241 tttcaacagt aaaggacaca tctttcaaaa caggaacatg aacatagcca cctgtcaggt
     9301 ttttaatttc taacatattt tctccaaatc tggtataatg tagctatatt atatcaaaat
     9361 tcagtacagt agaggtagat tttatgtcag attgcatttt ttgtaaaatc atcgcagggg
     9421 aaattcctgc ttcaaaagta tatgaagatg agcaggtcct tgcctttctt gatatctctc
     9481 aagtaacact aggacacacc ttggtcgtgc caaaagaaca ctatcgcaat cttttggaga
     9541 tgaatgctac gagcgccagc caactctttg cccaagtacc aaaagtagct caaaaagtca
     9601 tgaaagtcac taaggctgct ggtatgaata tcatttccaa ctgtgaagaa gtcgctggtc
     9661 aaacagtttt tcatactcac gttcaccttg tgcctcgcta cagtgctgac gatgacctca
     9721 agattgattt tatcgcccac gaaccagact ttgacaaact tgctcaagtc gctgaaacca
     9781 tcaaaaacgc ttaaggagtt gctatgaaat tatccaacct actgctattt gcaggagctg
     9841 cagccggaag ttatctggtt acaaaaaatc gccaaaccat cacagatgaa gtcttaaata
     9901 ccactgaccg cgttcaagct atcaaagacg atgtggatat tatccaaaat agcctgcaaa
     9961 tcatcgacca gcaaaaagaa cttatcaagg aataccaaga agacttgact tacaagttta
    10021 aggtcttgga aaaggatatc cagactagac tagctgtgat aaaagaaatg cagggaactg
    10081 aagataagta aaaagagccc ccggctcttt tttaaaacgt aaacaagtta tagtgaattg
    10141 aatttagaat agcacatcat agtttctaaa gcattgttag agattacttt aaaatcctct
    10201 tatcaatttg ttcatattct atttcaatct actatatata tcccatcaac tatgcatcat
    10261 aatttaggta actcatactc aataaaaatc aaaaagcaaa ctagaaagct aggcacagac
    10321 tgctcaaaac accgttttaa ggttgtggat agaactgacg aagtcagctc aaaacactgt
    10381 tttgaggttg tagatacgac tcacatatat acggcaagcc gacgctgacg tggtttgaag
    10441 agattttcga agagtattat tctccatcaa aggcatcttt aatatggtca aagaagcctt
    10501 ttttctttgg atttactttc aagtcaccag cagccgcgaa ttctttcaag gctacttttt
    10561 ggcggtcgtt caagcctgtc ggtgttacga cattaacagt aacgtattgg tcaccaactg
    10621 caccgccacg aaggctcggt gcccccttac tacgtaggcg gaatttctta ccagtctgag
    10681 ttccctctgg aataaccaat tcaacatcac cgtgaacagt tggaatatct actgtatcac
    10741 caagagccgc ttggacaaag ttgaggttga gattgtagaa gatagtcgtt ccttcacgtt
    10801 caaacttgtc gctagcttct acagaaacta ctacatacaa gtcaccataa ggtccaccgt
    10861 taaagcctgc ttcaccttga ccagcgaggc gaatttgttg acctgtttcc acaccagcag
    10921 ggattttcac atgtacgcta tgagcttgtt tctcatgacc tgttccatga caggttgtac
    10981 atggatattt gatttctttt cctcgaccgt gacagacatc acaggttact tggcgacgca
    11041 tcataccaag aggagtctgc gtatcgacgt taatgacacc agcgccatga cagcgtccac
    11101 aagtgactgg acttgtccct ggcttagcac cagagccatt acatgtacga cagccagctt
    11161 cacgatgata cttaacttcc ttctcagttc cgaagatagc ttcttcaaag gtcaaattga
    11221 cacgatactg gagatcatct ccttggcgag gagcgtttgg attgcgcgaa gaaccgcctc
    11281 cgccgaagaa acttgagaaa atatcctcaa aaccaccgaa gccacctgcc ccattgaaac
    11341 cgccgaaacc accagctcca ccaaaaccac cattggcgcc tgcagcacca tactggtcat
    11401 aggcagcacg tttttggtcg tcactcaaag tctcataggc ttcttgaact tccttgtact
    11461 tgtcctcagc accaggctcc ttgttgatat ctgggtgata ttttttggaa agcttacgat
    11521 aagccttttt gatctcgtct gccgaagcgt tttttgacac ccccagacga tcataaaatt
    11581 cagtattgtt catacaagat accaagaccg taaaattcga ctgaaaaata ggaaatctga
    11641 cgctggagcg acgctcctag acagatttct cttttttccg agaatttagg tcgggttcaa
    11701 ttcctttctt ttatattgat gagataatac tccagccact cggccctatc cctttttacc
    11761 gaaaaacaga ggctgggttg caacctctgt ggttagatta ttcatttgaa ataaaaatgg
    11821 ttgtattatc tctagtagta atcttcaaag accttttagc taaatcaaac tcaaatttat
    11881 tatagtgtgg gtcttgtgtt ttatataggt gtaactcctg acatatatga tccgaaaagt
    11941 aaatcataac atctttaaac acagaagtgt agtcatattt actcttacca acagctactt
    12001 ctgtaaattc tttataaatg acatttccat ttctttccac agtcaccagc atttctgttg
    12061 gattataatc caaattaaat aattcttcaa aaaactttat cttttcatga taatctgcaa
    12121 tgtcagaaag attattttta ttaacactca taacactagc cccttacttt tccgtaaact
    12181 ctccgtctac gacgtcatcg cctgcgtttc ctgttgcttg tgcgccttct gctccttctt
    12241 gagcttgttg cgctgctgcg gcttgttcgt agagtttaac agcaagtcct tgagcttttt
    12301 cgttcaatgc ttcaagtttt gctttcatgt cgtccaagtt gttgtcttct tgagctttct
    12361 taaggtcatc aagggcagct tgggcagcgt cacgttctgc gtcgaagcct ttaccttcag
    12421 tttccttgat tgtcttttca gtcgcaaaga ttgcttggtc tacttcatta cgaaggtcta
    12481 cttcttcttt acgtttctta tcggcttcag cgtttgcttc tgcatctttc atcatgcggt
    12541 cgatttcttc gtcagtcaaa cctgagttcg attggatgac aatagtttgt tctttttgag
    12601 ttccaaggtc tttggcctta acagacacga taccgttctt gtcgatatca aatgttactt
    12661 cgatttgagg aattccacga ggtgcagctg ggatatcagt caattggaag cgtccaagag
    12721 tcttgttatc tgctgccatt gggcgttcac cttgaagaac gtggatatca acggctggtt
    12781 ggttgtctgc tgctgttgag aagacttgtg atttagatgt tgggattgta gtgttgcgat
    12841 cgataagttt tgtaaatact ccacccattg tttcgatacc aagtgacaat ggcgttacat
    12901 caagaaggac aacatccttg acatcaccag taatcacacc accttggata gccgcaccca
    12961 tagcaactac ttcatcaggg tttactgatt tgtttggttc tttaccagtt tcagctttaa
    13021 cagcttcaac aacggcaggg atacgagttg aaccaccaac aaggataact tcgtcgattt
    13081 ctgacaagct caaacctgca tctgaaaggg cttgacgaac tggaactttt gtacgttcaa
    13141 caaggtcacg agtcaaatca tcaaatttcg cacgagttaa agtcatttcc aagtgaagag
    13201 gtccagcctc acctgcagtg ataaatggca agctgatttg tgttgaagtt acaccagaaa
    13261 ggtctttctt cgctttttca gccgcatctt tcaaacgttg cattgccatc ttgtcagtag
    13321 acaagtcgat accgttttct ttcttgaatt ctgctaccaa gtggtcaatg attttttggt
    13381 caaagtcgtc accaccaagt ttgttgtccc ctgcagttga caatacgtcg aagacaccgt
    13441 cacccaattc aaggatagag acgtcgaatg taccaccacc aaggtcaaat accaagattt
    13501 tttcttcttt gtcagtcttg tccaaaccat aagcaagagc tgctgcagtt ggttcgttaa
    13561 caatacgttc tacttcaaga ccagcaattt taccagcgtc ttttgttgct tgacgttgag
    13621 cgtcgttgaa gtaagccgga actgtgataa cagctttggt tactttctca ccaaggtagt
    13681 cttcagcgta gcctttcaag tattgaagga tcatagctga gatttcttgt ggagtgtatt
    13741 cttttccatt tgcagaaact ttttcagaag ttcccatctt agatttgata gagataactg
    13801 tatctgggtt tgtgactgct tgacgttttg cagcatcacc aacgatgatt tctccgtttt
    13861 tgaatgagac tacagatgga gttgtgcggt ttccttctgg gtttgcgatg attttgcttt
    13921 cagttccttc aagaactgca actgctgagt ttgttgtacc taagtcaata ccgataattt
    13981 tagacatgtg tttttctcct tgttagttaa ttttctattt ttctttttga tactcttctt
    14041 aagtggcgac gccgtcagag cttgacttcg tcaatctctt tgactaaact ttgagcctaa
    14101 ggtctcaaaa tttgcgcaaa tagcgccacg gcgaagagta tcgtgtttgg ttcgcttcgc
    14161 tcactattac aaagccaaac atattttatc tttcttcata gtttattgtc gtttcggaca
    14221 agttttctat tatgtaaatt gcgacacgaa gagtcgaaat cgattttatt tcgacgacga
    14281 gttagtaagg aagctaggca aacgccatag cgattgccgt tttctgacga gttgctttag
    14341 ctaccgtcag aattgccgaa tcgaacacgc cctaagctct gagtaaaaaa gatagatttt
    14401 cttgtgttca tcgaacactt cgtcaatctc ctatttttac tgcgagcttt ttacgggctt
    14461 tgtatcttag ttatacacca ctaccattgc tgggcgtagg atgcggtcat ggagtttgta
    14521 gcctttttga aagacttgag cgatggtatc tactgggtgt tcatcgtctg ctgggagagt
    14581 ttggatggcc atatggtagt tatggtcaaa ttcgccatct gctgcgattt cttcaattcc
    14641 ttcttctttc aaagcgtgaa tcaagctttc ttgcaccatc cccaagccct tcttcacatc
    14701 atctgtcaaa ccttcaactg caagtgcacg ctcaaggttg tcaagagatg gtaagattgc
    14761 ttttgccaag tcctggctac gataacgttg caagttttga cgttcttcat tggcacggcg
    14821 ttggatattt tgcatttctg catgagcgcg aagatatttg ttttcgaact catctgcacg
    14881 ttcatttgcc aagtccaact cagacttttc aggagttgtt tcttcagctg tttccacaac
    14941 ttcctcttct tgaacttctt ctacttcttc attttttata tcttgggcca tgttcgcctc
    15001 ctttaatgat ttcaatctta atgtacttcg taatgattac tgctgaggta gcggtaaaaa
    15061 tctgtcaact tcatggtcaa aacacggttg accacattga tttgattgat tagctgttgg
    15121 taatccagat taactggacc gataatggct agaattccaa ctccccgata aggaatgagg
    15181 aacttactac taatcaccgc taggtcagct aaacaggact cttgaccgtc tgcaatacga
    15241 acattttgca tctgatcctc acgcaacccc tcacgaatct ccaaggccac cttttgcggt
    15301 tggtcaaaga actgataggc tgctagattg gcaaaattca agagattgac cttgcccgcc
    15361 atcacaatgt tttcgttgaa catttcctta aagatgtgtt caaagagatc gatgacatta
    15421 tccgttgttg taaagtaacg ctggataatc tgcggaatct ccgtccgaat cttgtagtga
    15481 atatctaaaa cggtgtgacc gaggaaacgt tcctgaatga tgctcttcag tttcagcaaa
    15541 tcctcctgca agaagttcct tggaatcaga aactgactag taaccgttcg cgactcgtct
    15601 agggtaaata ccgccaaggc tgtatgttgc cccaaaacaa cgatatcaaa ggctgtcaaa
    15661 cgttgcctgc tcggctcaac atccagtgcc actaccgtac agccactcag gtctgttagc
    15721 aagttagcag cctcttgcag aatatcctcc aatttgaaga attcctgatc aaaggctttg
    15781 acaatctcat atacctcatt ttcagccagc cggtgaaaat ccagtgagtg tttcacatag
    15841 tactgaaaac cagcaacact tggcatccga ccgcttgaag tatgagcctt ctcaagcaac
    15901 ccttgttttt ctagttccgc catgtcatta cgaatggttg cactgctaga gttaatagac
    15961 tcttgcaagg cttttgatcc gacaggttcg tgcgttttgg taaagatgtc aataatcaga
    16021 tttaaaatat cctgctgacg ctctgtaacc atctcatcac tcctctctgt ctgatcaatt
    16081 agcactcaat gcatccaagt gctaacttat gattctatta tacaccctta gaagagaatg
    16141 tcaagacaaa aactcaaaaa attagcactc ttttatcaag agtgctaaaa atcaatctga
    16201 agacctagaa ccttgctatt catctactcg gtatttcctt tttagtctga aataaccatg
    16261 aattagataa gaaatcagaa aagcatataa aacaaaaata agaaagaaag acatagaaag
    16321 atcctctcta aacaaactcg tacaaacaac aagaccacct aagaaagtag ttgtacagga
    16381 acgaagtcta gatcgcataa cttcccattt gagcccctta ctcatataga cttgatcctc
    16441 tggaagtaaa tcagaagacg atttacgaag aatcgaacaa gaacctgctc ctatcaattc
    16501 ccaacctcta tctctaaaat cttgcagttc atgcttatat tttttaagaa atctagaatc
    16561 atagatacgg tagatgacat cgtctggttg acattggtca aaatagaaca aaccaaaacg
    16621 actcgttcta tacctccaac ctttcaaatg catctcatgt aaatattctt cttccttgtc
    16681 caaatcaaca atagtgaaaa tccgaaattc tactctgcta ttcattgtct taccccaaaa
    16741 ttagaaaaca tgcctggcgt tatttattag ataattcttt ccgcttttga ctcaatctcc
    16801 aaaaaatata agaaatctga atcgcaaaaa ctatcaataa aacccaatct attatgaaag
    16861 tcaaaaacac ttcccaactg aaagaactac ctccagtgac aaactttgag aaaaacggta
    16921 gtagagctaa aaagagaagc aaaataggaa gcatccgcat tgttaaaatc cttttggcat
    16981 aaaaaaatct ttatttaaac gaaaatatta tggcaaaatt tacgccagtt tttgaacggc
    17041 tgatgtagat attttatact ttcaaaatgt ttaaatgtga ttatttattt ttgaaaaata
    17101 gctcaccagc ccgactgaaa gtgcttataa atgataataa gtcgcctgcc gaaaacagcg
    17161 aaaaatagcg gtgttatgcg gagataatct gacgcgatgc gaaagtatat tgcatactta
    17221 ttttcaacaa tttagcagag tatttttata agtgtgatat aatagaagta taatttgttc
    17281 tgatagttta ttttatggag aagtagattt ttagaatgcg gagggttcaa tatggttgag
    17341 tttataaagt ctaagaaaga aatgagtgag gaggatatta aagcaaattt catcactcct
    17401 gctattgtat ccaaaggatg gaaaaatggt gagcatatcg cttacgaaga atacttcact
    17461 gatggtcgaa ttgaggttag aggagataag gctcgtcgta aagaaggaaa aaaatcagac
    17521 tattcactgt attaccaatt tggaactcga attgcaattg ttgaggcaaa ggataataaa
    17581 cacagcgttc gagcaggatt acaacaagct attgaatatg gagagatttt agatgttcca
    17641 tttgtttatt cttcgaatgg tgatggcttt attgaacacg accgtatcac gagagaagaa
    17701 cgtgagctgg agttagacga attccctact cgtgaagaat tattttctcg tatgacgaag
    17761 gaaaaaggat tgacgtacga aattacagaa gctatctcaa ctccatacta tacagacgcc
    17821 ttctcaatga aaacgccacg ctattatcag caaatagcta tcaaccgtac tattgaaaca
    17881 gttgccagag gacaaaaacg agtaatgttt gtgatggcaa caggaacggg gaaaacgttc
    17941 atggcttttc aaattattca tcgccttcga aaagctggtt tggctaaacg agttttattc
    18001 ttagcggata gaaacatctt agtagaccaa acgatggctg aagactttag gccattcgaa
    18061 aaggtaatga cgaaaattac accaaaactt ttgactgctc ctgaaaaatt aaattctttt
    18121 gaaatttatc tagggcttta tcagcaacta actggtgaag atggaactga aacacattat
    18181 caaaaatttg acaaagactt ctttgattta atcgtaattg atgaagcgca ccgtggttca
    18241 gctaaggaaa acagtaactg gcgtaagata attgattatt tcagttctgc gacacagatt
    18301 gggatgaccg ctactcctaa agaaaccaag aatgcttcca atacggaata ctttggtgag
    18361 ccaatctata cttatagttt aaaacaggga atcgaggatg gttttttggc tccatatcgt
    18421 gttatgaggg ttaatttaga tgtggatgtg gatggttatc gtccagaaac tggaaaagtt
    18481 gatgctaacg gacaattaat agaagatagg tactacggca ggaaagattt tgataaaacc
    18541 attgtcattg atgatagaac gcaaagagtt gccaagtttg tttctgatta tatgaagcaa
    18601 aacaatgcac gatttgataa aacaattgtt ttttgtgttg atattgacca tgccgagcga
    18661 atgcgtgctg cacttgtaaa agagaatcta gacttagtcc aagaagacta tcgttatgtc
    18721 atgcaagtaa ctggtgacaa cgctgaagga aaagctcaac tggataactt tatggatgtc
    18781 aattctaatt ttcccgctat ggtaacaacg tctaaattat taacgacagg agttaatgct
    18841 aaaacatgtc gtttgattgt tttagactct aatatccaat ccatgactga atttaaacaa
    18901 attattggtc gtggcacacg tctttatcct caaaagggga aagaattttt tacgattatt
    18961 gattttcgaa atgttaccaa tttgtttgct gaccctgatt ttgatggtga tcctgtgaag
    19021 gtgctggaaa caggtgcgaa aacagtcagt ggttctacgc ccggtttcgt agataaggaa
    19081 ggtgacccag tagaaaaata tatcgttaca gacaagcagg ttaccattct taattctact
    19141 gttcaagtat tggatgaaaa cgggaaactg attaccgaaa gcctgaccga ctacactcgg
    19201 aagaatatct taggtagcta cgccactttg aacgatttta tcacagtttg gcatacggcg
    19261 gataaaaaga agcttatctt agacgaactt tataaaaaag gagtttatct agatgctatt
    19321 cgagagtcgg agggaatatc agaacaagaa atcgatgatt ttgatttact cctaaaactt
    19381 gcctatggtc aaaaagaatt aaccaaaacg gaacgtatca ataaactcaa acaaagcgga
    19441 tatttatata aatatagtga ggaagcgcgt gctgttttgg aaattttact gaacaaatac
    19501 atggataaag gtattggaga actcgaaagc attgaaacat taaaacttcc agaatttcag
    19561 atatatggtg gaaccttcaa aatcatcaat acttattttg gagataaaaa acgatattta
    19621 caagcaatta aagaattgga gcaagagcta tttacagtag cttaatgaaa ggaaagtatg
    19681 tcaattacat catttgtaaa aagaattcaa gatatcactc gaaacgatgc tggtgttaat
    19741 ggtgatgctc aacgtattga gcaaatgtct tggttattat tcttaaaaat ttatgatagc
    19801 cgtgaaatgg tttgggaatt agaagaagac gagtatgagt caattatccc agaggaatta
    19861 aaatggcgaa attgggctca tgctcaaaat ggggaacggg tattgacagg cgatgaatta
    19921 cttgattttg tcaataacaa gttattcaaa gagttgaaag agcttgaaat aacttcaaat
    19981 atgcctattc gaaaaacgat tgttaaatca gcttttgaag atgcgaacaa ctatatgaaa
    20041 aatggcgtct tgttacgcca agtcatcaat gttattgatg aagttgattt caatagccct
    20101 gaagatcgtc attcgtttaa tgatatttac gaaaaaattc ttaaagatat tcaaaatgct
    20161 gggaactcag gagaatttta tacgccacgt gcagcgactg attttattgc cgaagttctt
    20221 gacccaaaac ttggagaatc aatggcagac cttgcttgcg gaacaggagg cttcttgact
    20281 tcgactctga accgtttaag tagtcaacgt aaaactagtg aagataccaa aaaatataat
    20341 acagctgttt ttggtattga aaagaaagca tttcctcatc ttttagcagt tacaaatctg
    20401 tttcttcacg aaattgatga ccctaaaatt gttcatggaa atactttgga gaaaaatgtt
    20461 cgtgaatata cgaatgatga aaaatttgac attattatga tgaatccacc ttttggaggg
    20521 tcagaattag aaacaataaa aaataacttt ccagcagaat tacggagttc tgaaacagct
    20581 gatttattta tggctgtcat tatgtatcgt ttgaaagaaa atggtcgtgt tggagttatt
    20641 ttacctgatg gttttctatt tggtgaaggt gtaaaaactc gcttgaaaca aaaactggta
    20701 gatgagttca acttgcatac gattattagg ttgcctcata gtgtctttgc accgtataca
    20761 ggaatccata cgaacattct tttctttgat aaaacaaaga aaacagaaga aacttggttt
    20821 tatcgtttag atatgccaga tggttataaa aatttctcga aaactaagcc gatgaagtca
    20881 gaacacttca atcctgttcg tgactggtgg gaaaatcgtg aagagattct ggaaggtaag
    20941 ttctacaaat ctaaatcatt tacacctagt gaattggctg agttgaatta taatttagac
    21001 cagtgtggtt ttccaaaaga ggaagaggaa atcttaaatc cctttgagtt gattcagaat
    21061 tatcaagcgg aaagagcaac tttaaatcat aagattgata atgtattagc tgatattttg
    21121 cagttgttgg aggacaaata atgacaccag aacaacttaa agcaagtatt ctccaaagag
    21181 cgatggaagg gaaattagtg ccgcaaaatc ccaatgacga acctgcaagt gaattattaa
    21241 agagaattaa agctgaaaaa gaaaaactta tcagtgaagg aaaaatcaaa cgagataaaa
    21301 aggaaactga gatatttcgt ggtgatgatg ggaaacatta tgggaagttt gctgatggaa
    21361 gtactcaaga aattgatgtt ccttatgata ttcctgatac ttgggagtgg gtgaggtttt
    21421 ctacattggt tgaaattgtc agaggtggct ctccacgacc aatcaaagat tatcttactt
    21481 ctgaagtaga tggaataaat tggataaaaa taggtgatac tgaaaagggt gaaaagtata
    21541 taaataatgt taaagaaaaa atcaaaaaat cagggcttaa caaaactaga tttgtaaaaa
    21601 aaggtacatt tttgttaact aattctatga gttttggtag accttatatt ttgaatgttg
    21661 atggtgcaat acacgatgga tggttggcta tttcgaacta tgaaaactca ttaaataaag
    21721 attatctatt ctatattctt tcatcaaatg tagtttattc tcaatttcta tctctaatta
    21781 gtggagctgt tgtgaaaaac ttgaatagtg ataaagttgc ttctattctt atccctctcc
    21841 ccccactatc cgaacaacaa cgcataatag aagcaatcga atcagcttta gaaaaagtag
    21901 atgaatatgc tgaaagttat aatagactag aacagctaga taaagaattt ccagataaac
    21961 taaaaaaatc tattcttcaa tatgctatgc aaggaaaatt agttgaacaa gacccaaatg
    22021 atgaatcagt cgaagtttta cttgaaaaaa tacgagcaga aaaacaaaaa ctctttgaag
    22081 aaggcaagat taaaaagaaa gatttggaca tttctattgt ttcccaagga gatgataact
    22141 cttattatga ggaagtacct tgtgaaatac cagaaagttg ggagtgggtg aggttaaacg
    22201 atataacctc ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc
    22261 ctgtgattgc tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta
    22321 ttgatcccga aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa
    22381 tgtggaattc aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa
    22441 atccttatgg ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcgggag
    22501 ttattaattg ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg
    22561 aagaaaaagc gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat
    22621 atttaatccc cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat
    22681 ttttcgccca tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta
    22741 tcaaacataa gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg
    22801 tttagctgaa aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta
    22861 cgaactcaaa atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc
    22921 taaaactgtc aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga
    22981 tgatttgaaa ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag
    23041 ccatgctgat tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca
    23101 ttttgtcgtt tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa
    23161 agtagagcat gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag
    23221 actgtacatt cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg
    23281 ccgaagtggt tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc
    23341 tcaacagtta aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca
    23401 ttcttttaga catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct
    23461 tgcagatttg atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc
    23521 tactgaacaa caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca
    23581 aactgactac ctgctatttt tgtgattatg gctcttatta tgggaacata cctatgaatt
    23641 gggttgttat aaaaataaaa gatatttttt caataaatac aggtctttct tacaagaagg
    23701 gcgatttaag cattaataaa ggtgttagaa ttatacgtgg tggtaatatt aagcctttag
    23761 aattttctct gttggataat gattactaca ttgacacaca attcatctcc tctgagcaag
    23821 tttatttaaa acataatcag ctaataacac ctgtatcaac ctctttagaa catattggaa
    23881 agtttgcaag aatcgataaa gactatgatg gtgttgtggc tggtggattt attttccaat
    23941 taacaccatt cgaaagttca gagattattt caaaatttct attatttaac ttgtcctctc
    24001 cgttatttta taaacaattg aaagcaataa ctaaactatc aggtcaagct ttatataata
    24061 ttcctaaaac tacactaagc gagctattaa ttccgttagc tccttttgag gaacaggaac
    24121 ttattactca aaaagttgag aaactttttg aaaaagtaaa tcaactttgg aaatgattct
    24181 tttcatctct tcatgattag aaatagggat taataattcg gagatactgg tactatttaa
    24241 tgttttccct ttgatagcat cttttgaatc acctaaagta gagataagtg gcaaaaatat
    24301 cattaagtaa tctctgataa tattttcttt attagcatag gggaatatcg atataatggc
    24361 ttcattatga gtggcaggaa tatccaatat ggcaactttt ccaatagata atttaaaact
    24421 cattaataaa gttcctttag gtgaaatgtc tattttcttt gattttaatg ctaatttaga
    24481 aatagattct ctcgtattag ttacataacc agatataggc atatctgata tagataccca
    24541 gggtatttca gttccccaaa aagtagcttc actgcgtgga ggagtttttc ctattctgaa
    24601 gttaactagg ctagcaaatt taatatatct ccatgctttt gggatttcat atataggata
    24661 agaggttgtt tcgtctttgt tc
//
LOCUS       DAVLXF010000023        24100 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLXF010000023 DAVLXF010000000
VERSION     DAVLXF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.023"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            650..2803
                     /locus_tag="VYO12_000870"
     CDS             650..2803
                     /locus_tag="VYO12_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1587510.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLFSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNSLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2816..4165
                     /locus_tag="VYO12_000871"
     CDS             2816..4165
                     /locus_tag="VYO12_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1587511.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQSEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQFFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4335..5042
                     /locus_tag="VYO12_000872"
     CDS             4335..5042
                     /locus_tag="VYO12_000872"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1587512.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIVG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5244..8969
                     /locus_tag="VYO12_000873"
     CDS             5244..8969
                     /locus_tag="VYO12_000873"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1587513.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFTQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVCVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9062..10504
                     /locus_tag="VYO12_000874"
     CDS             9062..10504
                     /locus_tag="VYO12_000874"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1587514.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10541..11563
                     /locus_tag="VYO12_000875"
     CDS             10541..11563
                     /locus_tag="VYO12_000875"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1587515.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11560..12105
                     /gene="purN"
                     /locus_tag="VYO12_000876"
     CDS             11560..12105
                     /gene="purN"
                     /locus_tag="VYO12_000876"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1587516.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12189..12698
                     /locus_tag="VYO12_000877"
     CDS             12189..12698
                     /locus_tag="VYO12_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1587517.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12723..14270
                     /gene="purH"
                     /locus_tag="VYO12_000878"
     CDS             12723..14270
                     /gene="purH"
                     /locus_tag="VYO12_000878"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1587518.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDATYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14392..15654
                     /gene="purD"
                     /locus_tag="VYO12_000879"
     CDS             14392..15654
                     /gene="purD"
                     /locus_tag="VYO12_000879"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1587519.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKTFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15845..16336
                     /gene="purE"
                     /locus_tag="VYO12_000880"
     CDS             15845..16336
                     /gene="purE"
                     /locus_tag="VYO12_000880"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1587520.1"
                     /translation="MTKPIISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTP
                     DLMFKHAEEAYSRGIKVIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSN
                     ELI"
     gene            16323..17414
                     /gene="purK"
                     /locus_tag="VYO12_000881"
     CDS             16323..17414
                     /gene="purK"
                     /locus_tag="VYO12_000881"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1587521.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEVD
                     LEAAYALAESADCVLEEFVNFALEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17424..17651
                     /locus_tag="VYO12_000882"
     CDS             17424..17651
                     /locus_tag="VYO12_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1587522.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            17714..19012
                     /gene="purB"
                     /locus_tag="VYO12_000883"
     CDS             17714..19012
                     /gene="purB"
                     /locus_tag="VYO12_000883"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1587523.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19067..23005)
                     /gene="strH"
                     /locus_tag="VYO12_000884"
     CDS             complement(19067..23005)
                     /gene="strH"
                     /locus_tag="VYO12_000884"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1587524.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKADVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYDDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKIISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAVTHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQEEDIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKVLPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23318..24034)
                     /locus_tag="VYO12_000885"
     CDS             complement(23318..24034)
                     /locus_tag="VYO12_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1587525.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIEFISCDRDH"
BASE COUNT         6568 a         4779 c         5726 g         7027 t
ORIGIN      
        1 ttaattagaa aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa
       61 taatcaaaac tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaagaggagt
      121 cttatagtaa cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct
      181 attctgacta ggaatagatc ataccagagg tagcttagaa atagcagaga cattagaaat
      241 tgaagtaata aatgggatgt cgtaagtgtt actatcaatg atttatttgt ttcaagcttg
      301 cctagggtga cagtaaaaaa tcaatttcct ttcaatagca tatttttagt gggcaggact
      361 cttgttctgc ctattttttt atccaaaaag tgcagttggg agggagatag gctcatttgg
      421 gaaggaagtc cagtttttgt ttagtgattg gggtaagata gttgttatca gatgagttta
      481 tactcttcga aaatcaaatt caaaccacgt caacgtcgcc ttgccgtata tatgtgactg
      541 acttcgtcag tcctatctac aacctcaaaa cagtgttttg agcagcctgc ggctagtttc
      601 ctagtttgct ctttgatttt cattgagtat tagggaaaag gagatgaata tgaaatttgg
      661 gaaacgtcac tatcgtccgc aagtggatca gatggactgc ggtgtagctt cattagccat
      721 ggtttttggc tactatggta gttattattt tttggctcac ttgcgagaat tggctaagac
      781 gaccatggat gggacgacgg ctttgggctt ggtcaaggtg gcagaggaga ttggttttga
      841 gacgcgagcc attaaggcgg atatgacgct ttttgacttg ccggatttga cttttccttt
      901 tgttgcccat gtgcttaagg aagggaaatt gctccactac tatgtggtga ctgggcagga
      961 taaggatagc attcatattg ccgatccaga tcccggggtg aagttgacta aactgccacg
     1021 tgagcgtttt gaggaagaat ggacaggagt gactcttttt atggcaccta gtccagacta
     1081 taagccttat aaggaacaaa aaaatggtct gctctctttt atccctatat tagtgaagca
     1141 gcgtggcttg attgccaata tcgttttggc aacactcttg gtaaccgtga ttaacattgt
     1201 gggttcttat tatctgcagt ctatcattga tacctatgtg ccagatcaga tgcgttcgac
     1261 actagggatt atttctattg ggctagtcat cgtctacatc ctccagcaaa tcttgtctta
     1321 cgctcaggag tatctcttgc ttgttttggg gcaacgcttg tcgattgacg tgattttgtc
     1381 ctatatcaag catgtttttc acctccctat gtccttcttt gcgacacgca ggacagggga
     1441 gatcgtgtct cgttttacag atgctaacag tatcatcgat gcgctggctt cgaccatcct
     1501 ttcgattttc ctagatgtgt caacggttgt cattatttcc cttgttctat tttcacaaaa
     1561 taccaatctc tttttcatga ctttattggc gcttcctatc tacacagtga ttatctttgc
     1621 ctttatgaag ccgtttgaaa agatgaatcg ggataccatg gaagccaatg cggttctgtt
     1681 ttcttctatc attgaggaca tcaacggtat tgagactatc aagtccttga ccagtgaaag
     1741 tcagcgttac caaaaaattg acaaggaatt tgtggattat ctgaagaaat cctttaccta
     1801 tagtcgagca gagagtcagc aaaaggctct gaaaaaggtt gcccatctct tgcttaatgt
     1861 cggcattctc tggatggggg ctgttctggt catggatggc aagatgagtt tggggcagtt
     1921 gattacctat aataccttgc tggtttactt taccaattct ttggaaaata tcatcaatct
     1981 gcaaaccaag cttcagacag cgcaggttgc caataaccgt ctaaatgaag tgtatctagt
     2041 agcttctgag tttgaggaga agaaaacagt tgaggatttg agcttgatga agggagatat
     2101 gaccttcaag caggttcatt acaagtatgg ctatggtcga gacgtcttgt cggatatcaa
     2161 tttaaccgtt ccccaagggt ctaaggtggc ttttgtgggg atttcagggt caggtaagac
     2221 gactttggcc aagatgatgg ttaattttta cgacccaagt caaggggaga ttagtctggg
     2281 tagtgtcaat ctcaatcaga ttgataaaaa agccctgcgc cagtacatca actatctgcc
     2341 tcaacagccc tatgtcttta acggaacgat tttggagaat cttcttttgg gagccaagga
     2401 ggggacgaca caggaagata tcttacgggc ggtcgaattg gcagagattc gagaggatat
     2461 cgagcgcatg ccactgaatt atcagacaga attgacttcg gatggggcag ggatttcagg
     2521 tggtcaacgt cagagaatcg ctttggcgcg tgctctcttg acagatgcgc cggtcttgat
     2581 tttggatgag gcgactagca gtttggatat tttgacagag aagcggattg tcgataatct
     2641 catagctttg gacaagacct tgattttcat tgctcaccgc ttgactattg ctgagcggac
     2701 agagaaggta gttgtcttgg atcagggcaa gattgtcgaa gaaggaaagc atgctgattt
     2761 gcttgcacag ggtggctttt acgcccattt ggtcaatagc tagaaagagg agaggatgaa
     2821 accagaattt ttagaaagtg cggagtttta taatcgtcgt taccataatt tttccagtag
     2881 tgtgattgta cccatggccc ttctgctcgt gtttttactt ggctttgcaa ctgttgcaga
     2941 gaaggagatg agtttgtcca ctagagctac tgtcgaaccc agtcgtatcc ttgcaaatat
     3001 ccagtcaact agcaacaatc gtattcttgt caatcatttg gaagaaaata agctggttaa
     3061 gaagggggat cttttggttc aataccaaga aggggcagag ggtgtccaag cggagtccta
     3121 tgccagtcag ttggacatgc taaaggatca aaaaaagcaa ttggagtatc tgcaaaagag
     3181 cctgcaagaa ggggagaacc actttccaga ggaggataag tttggctacc aagccacctt
     3241 tcgcgactac atcagtcaag caggcagtct tagggctagt acatcgcaac aaaatgagac
     3301 catcgcgtcc cagaatgcag cagctagcca aacccaatcc gaaatcggca acctcatcag
     3361 tcaaacagag gctaaaattc gcgattacca gacagctaag tcagctattg aaacaggtgc
     3421 ttccttggcc ggtcagaatc tagcctactc tctttaccag tcctacaagt ctcagggcga
     3481 ggaaaatccc caaactaagg ttcaggcagt tgcacaggtt gaagcacaga tttctcagtt
     3541 agaatctagt cttgctactt accgtgtcca gtatgcaggt tcaggtaccc agcaagccta
     3601 tgcgtcaggg ttaagcagtc aattggaatc ccttaaatcc caacacttgg caaaggttgg
     3661 tcaggaattg acccttctag cccagaaaat cttggaggca gagtcaggta agaaggtaca
     3721 gggaaatctt ttagacaagg ggaaaattac ggcgagtgag gatggggtgc ttcatcttaa
     3781 tcctgagacc agtgattcta gcatggttgc agaaggtgcc ctactagccc aactttatcc
     3841 atctttggaa agagaaggga aagccaaact cacagcctat ctaagttcaa aagatgtagc
     3901 aagaatcaag gtcggtgatt ctgttcgcta tactacgact catgatgccg ggaatcaatt
     3961 ttttctagat tctactatta caagtattga tgcgacagct actaagactg agaaagggaa
     4021 tttctttaaa atcgaggcgg agactaatct aacttcggag caggctgaaa aacttaggta
     4081 cggggtggaa ggccgtctac agatgattac gggcaagaaa agttatctac gttattattt
     4141 ggatcaattt ttgaacaaag agtaatgttc gtgtttttag agttaaataa tttttaaact
     4201 gtgagaaaga ttcttcttgc agttttttct ttacaatttt tgaaaaacat atgctattta
     4261 ttcggttaaa ttcttgtgtt ttttggtttt ttgtggtaaa atgtgctcaa gtaatacgaa
     4321 aggcgaactt taaaatgtca aaacaattga tctattcggg aaaagctaaa gatatctata
     4381 caactgagga tgaaaatctt attatttcaa cttacaagga ccaggcgact gctttcaacg
     4441 gtgtcaagaa ggagcagatt gtaggtaagg gagtcttgaa taatcagatc tcatctttta
     4501 tttttgagaa attaaatgcg gctggtgtgg cgactcactt tgtggagaaa ctttcagaca
     4561 cggaacaact caataaaaag gttaagatta ttcctttgga agtcgtgctc cgcaactata
     4621 ctgctggttc cttttcaaaa cgttttggtg tggatgaggg aatcgccttg gagactccga
     4681 ttgtcgaatt ttactacaaa aatgatgatt tggatgatcc atttatcaat gatgagcatg
     4741 tgaaattcct acagattgcg ggtgaccagc agattgccta cttgaaggaa gaaacgcgtc
     4801 gtatcaatga actattgaaa gtctggtttg ctgagattgg gcttaaattg attgacttta
     4861 agctagagtt cggttttgac aaggatggca agattatctt ggcagacgaa ttttcaccag
     4921 ataactgccg cttgtgggac gctgatggca accacatgga taaggatgtt ttccgtagag
     4981 gattgggaga actaaccgac gtttatgaga ttgtttggga aaagttgcag gaattgaaat
     5041 aatctgtttg caacggaaaa ccttcgtctc tcaactaaaa ggactcaggc tgaaaaggtc
     5101 ccccagacct tttcactctg tagagaacta ggtgaactaa cagatgttta cgaaattgtc
     5161 tgggaaaagt tgcagggttt aaaataacaa cctcaaggct gtttgggaat attgcaagag
     5221 ctgaaataaa ggaataagaa ttgatggata aacgtatttt tgttgaaaaa aaggctgatt
     5281 ttcaggtcaa gtcagagagt ttggttagag agctccagca caacttggga ctgtcaagct
     5341 tgaaaagtat tcgtattgtg caagtatatg atgtatttga cttggctgag gacttgtttg
     5401 cacctgcaga gaagcacatt ttctctgagc agggaaccga ccatgtttta gatgaagtat
     5461 ctgtgcaggc ggatcttgct aactatgctt tctttgccat tgaaagtctg ccagggcagt
     5521 ttgaccagcg tgcagcttcg tcacaggaag ccttgctttt gttgggaagt tcgagtgacg
     5581 tgacagtcaa cacagcccaa ctttacttgg tgaataaaga tattgatgcg actgagttgg
     5641 aagctgtcaa aaactacctg ctcaatccag ttgattctcg tttcaaggat atcacgacag
     5701 ggattgccaa gcaggagttt tcagagtcag acaagaccat tccaaaattg actttctttg
     5761 aaagctatac agcagaagac tttgctcgct acaaggccga gcaagggatg gccatggaag
     5821 tggatgattt gctctttatc caagactact ttaagtcaat cgggcgcgtg ccaactgaga
     5881 ctgaactcaa ggttttggac acttactggt ctgaccactg ccgtcatacg acttttgaga
     5941 cagagttgaa acacatcgac ttttcagctt ctaaatttca aaagcaattg cagtcaacct
     6001 atgacaagta tattgccatg cgcgaggagt taggtcggtc tgaaaaacca caaaccttga
     6061 tggatatggc gactattttc ggtcgttatg agcgtgctaa tggacgattg gatgatatgg
     6121 aagtctctga cgaaatcaat gcctgctcag ttgaaattga agtggatgtt gatggtgtca
     6181 aggaaccttg gctcctcatg tttaaaaatg aaacccacaa ccatccaaca gaaattgagc
     6241 catttggtgg agcggctacc tgtatcggtg gagctattcg tgatccgttg tcaggccgtt
     6301 cctatgttta ccaagccatg cgtatttcag gtgctggtga tattacagca ccgatttcgg
     6361 aaactcgtgc tgggaaattg ccacaacaag tcatttctaa aacagcagct catggttatt
     6421 cttcatatgg taaccagatt gggcttgcca caacctacgt tcgtgaatac ttccacccag
     6481 gctttgtagc taaacgtatg gaacttggtg ccgttgttgg tgcggctccc aagggcaatg
     6541 ttgtccgtga aaaacctgaa gcaggtgatg tgatcatcct tctcggaggc aaaacaggtc
     6601 gtgatggtgt cggtggtgcg acgggctctt ctaaggttca aacagttgag tctgtagaga
     6661 ctgctggtgc tgaggttcaa aaaggaaatg ccatcgaaga acgcaagatt cagcgcctct
     6721 tccgtaatgg caatgtcact cgtctgatca agaagtccaa tgactttggg gcaggcggcg
     6781 tctgtgtggc tatcggtgaa ttggcagacg gtcttgaaat cgacctcaac aaggtgcctc
     6841 ttaaatacca gggcttgaat ggtacagaaa ttgccatctc tgaatcacaa gaacggatgg
     6901 cggtcgtggt tcgtcctgaa gatgtggata ccttcgttgc cgaatgtaac aaagaaaata
     6961 ttgatgctgt tgtggtggcg acagtaactg aaaaaccaaa tcttgtcatg cactggaatg
     7021 gtgagacaat cgttgacttg gagcgtcgtt tccttgacac caatggtgtg cgcgtggttg
     7081 tcgatgccaa agttgtggac aaggatgtca aactcccaga agagcgtcaa acatctgctg
     7141 aaacactgga atcagatacc cttacggttc tatctgacct caaccatgca agtcaaaaag
     7201 gattacagac tatctttgac tgctctgttg gacgctcaac ggttaatcac ccacttggtg
     7261 gtcgttacca actcacacca actgaggcat ctgtgcagaa attgccagtt caacacggtg
     7321 tgactcatac tgcgtcggtc attgctcaag gtttcaaccc atatgtagct gaatggtctc
     7381 cataccacgg tgctgcttat gcggttatcg aagcaactgc tcgtttggtg gctgctggtg
     7441 ccaactggtc caaggctcgt ttctcttacc aagagtattt cgagcgtatg gataaacaag
     7501 cagagcgttt cggtcagcca gtagctgctc ttctaggttc tattgaagca caaattcagc
     7561 ttggcttgcc atctatcggt ggtaaggact ccatgtctgg tacctttgaa gaattgaccg
     7621 ttccgccaac cttggttgcc tttggggtga cgacggcaga tagccgtaag gtgctctctc
     7681 cagaatttaa agctgttggg gaaaatatct actacatccc aggtcaagcc ctctctgcag
     7741 agattgattt tgacttgatt aagaaaaatt ttactcagtt tgaagccatc caagctgacc
     7801 ataaagtgac atctgcatca gctgtcaaat acggtggtgt agttgaaagt ttggctcttg
     7861 ctacctttgg aaactatatt ggtgcagagg tgaccttgcc tgaacttaaa acagctttga
     7921 cagctcaatt aggcggcttt gtcttcacat ctcctgaaga aattgctgga gtagagaagg
     7981 ttggacaaac gaaagcagac tttacactga ctgtcaacgg tgtgaagcta gatggacaca
     8041 agcttgacag tgcatttcaa gggacattgg aagaagttta cccaacagaa tttacccaag
     8101 cgaaagaact agaagaagta ccagctgtgg catcagatgt tgtgattaaa gccaaagaaa
     8161 aggttgaaaa acctgtggtt tacatcccag tctttccagg aaccaactca gaatatgatt
     8221 cagctaaggc cttcgaaaaa gaaggtgcag aggtcaattt ggtgccattc gtgaccttga
     8281 atgaagaagc tattgtcaag tcagttgaaa ctatggttga caatatcgac aagactaata
     8341 ttctcttctt tgctggtgga ttctcggctg cggatgaacc agatggttca gctaagttta
     8401 tcgtcaatat cctgcttaat gaaaaagtgt gtgtggctat tgatagcttt atcgcccgtg
     8461 gtggtttgat tatcggtatt tgtaatggat tccaagcctt agtcaaatcg ggtctcctac
     8521 cctacggaaa ctttgaagct gctaacagta ctagcccaac cctcttctac aatgatgcca
     8581 accaacacgt ggccaagatg gtggaaactc gcattgccaa taccaactca ccatggttgg
     8641 ctggtgtgca agtgggcgat atccacgcta ttcctgtttc gcacggtgaa gggaagtttg
     8701 tcgtgacggc tgaggaattt gcagagctcc gtgacaatgg acaaattttc agccaatacg
     8761 ttgactttaa cggtaaacca agtatggatt ctaagtacaa tccgaatggt tctgtccatg
     8821 ccatcgaagg aattaccagc aagaatggtc aaatcatcgg taagatgggc cactcagaac
     8881 gttatgagga tggtcttttc caaaatatcc caggcaataa agaccaacac ctgttcgcat
     8941 cagcggttaa acatttcact ggaaaataag acttacagat tttctaatag atagtatcag
     9001 taatgtaaaa gtcatgtaaa tctagctctt gatgattaca aatgaaaatt aggtataaaa
     9061 aatgacatac gaagtaaaat ctcttaatga agaatgtggt gttttcggta tttggggaca
     9121 tccagatgct gctaagttga cctattttgg actccacagt cttcaacacc gtggtcagga
     9181 gggggcagga atcctctcca atgatcaagg acaactgaag cgccatcgtg acatggggct
     9241 tttatcagaa gttttcagaa atccagccaa tttggataaa ttgacaggag ctggtgcgat
     9301 tgggcatgtg cgttatgcga ctgctggcga agcttctgtt gataacatcc aacccttcct
     9361 cttccgtttt cacgatatgc agtttggttt ggctcataat ggaaatctga ccaatgcagc
     9421 ctctctcaag aaagaactgg aacaaagagg agcaattttc agcgcgactt cggactctga
     9481 aatcttggct cacctcattc gtcgcagtca taatcctagc ctgatgggca aaatcaagga
     9541 agcgctcagc cttgtcaaag gtggttttgc ctatatcttg ctgtttgagg acaagttgat
     9601 tgcggctctt gaccctaatg gctttcgtcc gctttctatc gggaaaatgg ccaacggagc
     9661 ggtggttgtt tcctctgaaa cctgtgcttt tgaggtcatt ggtgccgagt ggattcgtga
     9721 tttgaagcca ggtgagattg tgatcattga tgacgagggc attcagtatg acagctatac
     9781 agatgatacc cagttggcga tttgttctat ggagtatatc tactttgccc gccctgattc
     9841 taatatccac ggtgtcaatg tccatacggc acgtaagaga atgggagcgc aattggcgcg
     9901 agaatttaag catgaggcag atattgtagt tggtgtgccc aattcttccc taagcgcggc
     9961 tatgggattt gcggaagaat caggcttacc aaatgaaatg ggtctgatca aaaaccaata
    10021 cacccagcga acttttatcc aaccgactca agaattgcgg gagcaaggag tgcggatgaa
    10081 actgtctgct gtttcgggtg ttgtcaaagg caaacgtgtg gttatggtgg atgattccat
    10141 tgtacgtgga acaacctctc gtcgtatcgt tcagctcttg aaagaagcgg gtgcgactga
    10201 ggttcacgtt gccattggaa gtcctgcact agcgtatcca tgtttctacg ggattgatat
    10261 ccagacccgt caggagctga ttgcagccaa tcatacggtc gaagaaactc gccaaatcat
    10321 tggtgcggac agtctgactt atctttcaat tgatagcttg attgagtcga ttggtatcga
    10381 aacagatgcg ccgaacggtg gtctctgtgt cgcttacttt gacggtgact acccaacgcc
    10441 tctctatgac tacgaagaag actatcgtag aagtttggaa gaaaagacca gtttttacaa
    10501 gtaggcgaca gattctccat taaagaaaag gaaaaaacaa atgacaaata aaaatgcata
    10561 tgcccagtcg ggtgtggatg ttgaagcggg ttatgaagtt gttgaacgga ttaaaaagca
    10621 cgtggcccgt acggagcgtg caggtgtcat gggagctctt ggtggctttg gtggtatgtt
    10681 tgacctttcc aagactgggg ttaaagaacc cgtcttgatt tcagggactg acggtgtcgg
    10741 aaccaagctc atgttggcta tcaagtacga caagcacgat accatcgggc aggactgtgt
    10801 ggccatgtgt gtcaacgaca tcattgctgc aggtgcggaa cccctctatt ttctcgacta
    10861 cgtagcgaca gggaagaatg aaccagctaa gctagaacaa gtggttgctg gtgtggcaga
    10921 aggttgtgtg caggctggtg ctgccctcat cggtggggaa acggctgaaa tgccgggcat
    10981 gtacggcgaa gacgactatg acttggctgg ttttgcggtc ggtgtggctg aaaaatctca
    11041 aatcattgac ggttcaaagg tggtagaggg agatgttctt ctcggacttg cttcaagtgg
    11101 gattcactca aatggttact ctttggttcg tcgtgtcttt gcggattaca caggtgagga
    11161 agtcctacca gaattggaag gcaagaaact taaggaagtt ctacttgagc cgactcgtat
    11221 ctatgtcaag gctgtcttgc cgctcatcaa agaagagttg gtcaacggca ttgcccacat
    11281 cacaggtggt ggctttatcg aaaatgtccc tcgtatgttt gcagatgacc tagctgctga
    11341 aattgatgaa agcaaagttc cagtgcttcc aattttcaaa gcccttgaaa aatatggtca
    11401 gattaaacac gaagaaatgt ttgaaatctt caatatgggt gtgggactta tgttggcggt
    11461 cagccctgaa aatgtagagc gtgtaaaaga attgttggat gaagcagtct atgaaattgg
    11521 tcgcatcgtc aagaaagaaa acgaaagtgt cattatcaaa tgaaaaaaat agcggttttt
    11581 gcctctggta atggctcaaa ttttcaggtg attgccgaag aatttccagt ggagtttgtc
    11641 ttttcagacc atcgtgatgc ctatgtgctt gagcgtgcaa agcagctcgg cgttctgtcc
    11701 tatgcttttg aactcaagga gtttgagagc aaggcagact acgaagcagc ccttgtcgaa
    11761 ctcttggaag aacaccagat tgacttggtt tgcctagcag gctacatgaa aatcgttgga
    11821 ccaaccttat tgtcggctta tgaaggtcgg attgtcaaca ttcatccagc ctacttgcca
    11881 gaatttccag gagctcatgg gattgaggat gcttggaatg ctggcgtggg tcagtctggt
    11941 gtgaccattc actgggtgga ttcgggtgtg gatacaggcc aggtcatcaa acaggttcgt
    12001 gtgccacgac tagctgatga taccattgac agatttgaag ctcgcatcca tgaagcagag
    12061 tacaggctgt atccggaagt agtgaaggct ctatttacag attgactttt tgatgattca
    12121 tatgatatct ttgattttaa attggagtca gtgtttgttg aagacggctt caaacggagg
    12181 tatttgtaat attagaatct aaaaaaacaa ctcgatatgt attttatgtc tatctgatgt
    12241 tattaacttg gggaatctta tttaagtttg aaacaaatcc tgaatttata gcatttttct
    12301 tagctccaag gtatatcaat tggattccat tttcagaacc actaatagtc gatggaaaaa
    12361 ttgtttttgc tgaaatgtta tttaatctga tttcctttat tccattaggt gtttgtttcc
    12421 ctttgataaa aactaattta tctagtttaa gaatagtcgg gacaggtttc ttgattagtt
    12481 tattgtttga gtgcttacag tatattttag caataggtat aacagatata acggatttga
    12541 ctttaaatac gctaggtgtc tgtgtaggct tactgattta tcaaattttt ataagagtgt
    12601 tcaaatcaca gactagaaaa tggatcaata tcttaggtat gcttagcctt ggttttgctt
    12661 atcttgtttt actgttactg catttaattg gtgtttaact aatgattaaa aaggagaata
    12721 taatgactaa acgcgtctta atcagcgtct cagacaaagc gggcattgtt gaatttgccc
    12781 aagaactcaa aaaacttggt tgggagatta tctcgacagg tggaactaag gttgcccttg
    12841 ataatgctgg ggtggatacc attgctatcg atgatgtgac tggtttccca gaaatgatgg
    12901 acggtcgtgt gaagaccctc cacccaaata tccacggggg gcttctcgct cgtcgtgact
    12961 tggatagcca cttggaagcg gctaaggaca acaagattga gctcattgac cttgtggtgg
    13021 tcaaccttta cccatttaag gaaactatcc ttaaaccaga tgcgacttat gctgatgcag
    13081 ttgaaaatat cgatattggt gggccatcta tgcttcgttc agcagcgaaa aatcatgcca
    13141 gtgttacagt tgtggtagat cctgctgact acgctgtggt tttggacgaa ttggcagcaa
    13201 acggcgaaac ctcttatgaa actcgccaac gtttagcagc caaagtattt cgtcacacag
    13261 cggcttatga cgccttgatt gcagaatact tcacagctca agtgggtgaa agcaagcctg
    13321 aaaaactcac tttgacttat gacctcaagc aaccaatgcg ttacggtgag aatcctcaac
    13381 aagacgcgga cttttaccag aaagctttgc ctacagacta ctccattgct tcagccaaac
    13441 agctcaacgg gaaagaattg tcatttaata atatccgtga tgcagatgct gctatccgta
    13501 tcatccgtga cttcaaagat agtccaaccg ttgtggctct caaacacatg aatccatgtg
    13561 gaattggtca agctgatgac atcgagactg cttgggacta cgcttatgag tctgacccag
    13621 tgtctatctt tggtgggatt gtcgtcctca accgtgaggt ggatgctgcg acagctgaga
    13681 agatgcacgg cgttttcctc gaaatcatca ttgcaccaag ctatacggat gaagcgctag
    13741 ccattttgat caataaaaag aaaaacttgc gtatccttgc cttgccattt aatgctcaag
    13801 aggctagcga agtggaagca gaatacacag gtgtagtcgg tggacttctc gtgcaaaatc
    13861 aagacgtggt caaggaaagc ccagctgact ggcaagtggt gaccaaacgt cagccaaccg
    13921 agacagaagc gactgctctt gagttcgctt ggaaggctat caagtacgtc aaatcaaatg
    13981 gtattatcgt gaccaacgac cacatgacac ttggtgttgg tccaggtcaa accaaccgtg
    14041 tggcttctgt tcgccttgcc attgaccaag ccaaagatcg tctggacggg gcggtccttg
    14101 cttcagatgc cttcttccca tttgcggata acgtggaaga aatcgctaaa gcaggaatta
    14161 aggccatcat ccagcccggt ggctctgtcc gtgaccaaga atccatcgaa gcagcggata
    14221 aatacggctt gactatggtc tttacaggtg tgagacattt tagacattaa gaagataaaa
    14281 gggaagaaaa cagtttcttt ccttttttgg cttaaaatgc taactgaaac aagattaaaa
    14341 cgaacttttt tgatataata ttggtaaata attcgcaaaa gaggttgagg aatgaaactg
    14401 cttgttgtcg gttcgggtgg tcgtgagcat gcgattgcta aaaagttact tgaatcaaaa
    14461 gatgtggaaa aagtctttgt agctcctggg aatgatggga tgactctgga tggtttggaa
    14521 ttggtaaata tctctatttc cgaacattct aaattgattg acttcgcaaa gaccaatgat
    14581 gttgcttgga cctttatcgg tccagatgat gcccttgctg ctggtatcgt ggatgatttt
    14641 aaccaagctg ggcttaagac ctttggtccg actagggctg cagcggagct ggagtggtcc
    14701 aaggattttg ccaaggaaat catggtcaaa tatggtgttt cgacagcagc ctatggcaca
    14761 ttttcagatt tcgaggaagc aaaggcttat atcgaaaagc atggtgcgcc tatcgtagtc
    14821 aaggcggatg gcttggcgct tgggaagggt gtcgtcgttg ctgagacggt tgagcaagcg
    14881 gtcgaagccg ctcatgagat gcttttggac aataaatttg gtgactcagg tgcgcgcgtg
    14941 gttattgagg aattccttga aggagaggaa ttttcactct ttgcctttgt caatggtgat
    15001 aagttctaca tcatgccaac ggctcaggac cacaaacgtg cctacgatgg cgacaaaggg
    15061 cctaacacgg gtggtatggg tgcctatgcg ccagttccac acttaccaca gagtgtagtt
    15121 gatacagcgg ttgacaccat tgtcaagcca gttctagaag ggatgattaa agaaggtcgc
    15181 ccttatctgg gagttcttta cgcagggctt atcctgacag ctgatggacc gaaagtcatc
    15241 gagttcaacg ctcggttcgg agatccagaa actcagatta tcttgcctcg cttaacctct
    15301 gactttgctc aaaatatcac agatatcctg gatagcaagg agccaaatat catgtggacg
    15361 gacaagggtg tgactctggg tgtggttgtc gcatccaagg gctacccgct agactatgaa
    15421 aggggcgttg agttgccagc caagacagaa ggcgatgtca tcacctacta tgcaggggct
    15481 aagtttgcgg aaaatagcag agcactgctc tcaaacggcg gacgagttta tatgctcgtt
    15541 accacagcag ataccgtcaa agaagcccaa gccagcatct accaagaact atcccaacaa
    15601 aaaatagaag gactcttcta ccgaacagat atcggaagca aggcaattaa gtaaagatat
    15661 aagaataacg cgccgtagtc gccaaacacg ataatggtcg tcgtggtgaa aagaccagaa
    15721 cagtgaatgt tctggtcagg gggaaatttg gagaccgtag gctcaaagtt taggaatgaa
    15781 accgaaggtt tgcttccgcc tccatcacct aagatcatta ttaaaaaaga agaaaaggaa
    15841 aattatgacc aaaccaatta tttccatcat catgggctca aaatccgatt gggcaaccat
    15901 gcaaaaaaca gcagaagtct tagaccgctt cggtgtagcc tacgaaaaga aagttgtttc
    15961 tgcccaccgt acacctgatc tcatgttcaa acatgcagaa gaagcatata gccgtggtat
    16021 taaggtcatc atcgcaggtg ctggaggcgc agcccacttg ccaggaatgg tagctgccaa
    16081 aacaaccctt cctgtcatag gggtacctgt caagtctcgt gctcttagtg gagtggattc
    16141 actctattct atcgttcaga tgccgggtgg ggtgcctgtt gcgaccatgg ctatcggtga
    16201 agctggagcg actaacgcag ctctctttgc cctccgtctc ctctctgtag aagataagtc
    16261 cattgcggat gcacttgcca actttgctga agaacaagga aaaatcgcag aggagtcgtc
    16321 aaatgagctc atctaaaaca atcggaatta tcggtggcgg tcaactgggt cagatgatgg
    16381 ccatttctgc tatctacatg ggccacaagg ttatcgcgct ggatcctgcg gcggattgcc
    16441 cggtctctcg tgtggcggaa atcattgtgg caccttataa cgatgtagac gccctccgtc
    16501 agttggcaga ccgttgcgat gtcctcactt acgagtttga aaatgtcgac gctgacggtt
    16561 tggatgccgt tatcaaggat ggacaactcc ctcaagggac agacctgctc cgcatttcgc
    16621 aaaatcgtat ttttgaaaag gactttttgt caaacaaggc tcaagtcact gtggcaccct
    16681 acaaggtcgt gacttctagc ctagacttgg cagatatcga cttgtcgaaa aactatgtcc
    16741 tcaagactgc gactggtggc tacgatggtc atggacaaaa ggttattcgt tcagaagtag
    16801 acttggaagc agcctatgcg ctagcagaat cagcagactg cgtcttggaa gaatttgtca
    16861 actttgccct tgagatttct gtcatcgtgt caggaaatgg caaggaggtg acgtttttcc
    16921 cagttcagga aaatatccac cgcaacaata tcctgtctaa gaccatcgta ccagcccgca
    16981 tttctgaaag tctagtagac aaggctaaag ctatggcagt gcgaatcgca gaacaactca
    17041 acttgtctgg aactctctgt gtggaaatgt ttgcgactga tgatgacgtt attgtcaatg
    17101 aaatcgcccc acgaccacat aactctgggc actattctat tgaagcctgt gatttctctc
    17161 agtttgacac ccatattctc ggtgttctcg gagcaccatt accagtcatc aaactccatg
    17221 cgccagccgt tatgctcaat gtcctaggtc aacacgtcga ggctgctgaa aagtatgtcg
    17281 cagaaaatcc aagcgcccac ctccacatgt atggtaaaat agaagcaaag cataatcgta
    17341 agatgggaca tgtgaccttg tttagtgatg taccggatag tgtggatgag cttggggaag
    17401 ggattgattt ttaggacaag tctatgatac aaattatcgt taatacattt attgaaaagg
    17461 ataagactgg agcagttgtt gaagtgttgt atgccagtgc tgaccaagat aaggtacaag
    17521 ctaaatatga agaactagct gcacaatacc ccgaaaatta tttagctatc tatgatgtac
    17581 cgctggatac ggatttgaat acactagatc attacccgtc tgtgtttatt ggaaaagagg
    17641 agtttgagta gaaatcttgg tttacctaga tagcttattc ccaacagctt aagaagaaag
    17701 gaaaaattaa cacatgatca accgttactc tcgccctgag atggcgaata tttggagtga
    17761 agaaaataaa taccgtgctt ggcttgaggt ggaaatcctc tctgacgagg catgggctga
    17821 gttgggggaa atccctaagg aagatgtggc tttgattcgc aagaaggcgg actttgacat
    17881 cgaccgtatt ttggaaattg agcaggagac gcgccacgat gtggtggctt tcacgcgtgc
    17941 ggtttctgag actcttggtg aagagcgcaa gtgggttcac tatgggttaa cttctaccga
    18001 cgtggtggat actgcttatg gttacctcta caagcaggcc aacgacatca tccgtcgtga
    18061 ccttgaaaac ttcactaata tcatcgctga caaggccaag gagcacaagt tcaccatcat
    18121 gatggggcgt actcatggtg tgcacgctga gccgacaacc tttggtctta aattggcaac
    18181 ttggtacagc gaaatgaaac gcaatatcga gcgcttcgag catgcggctg ctggtgtaga
    18241 agctggtaag atttctggtg cggttgggaa ctttgccaat atcccaccat ttgtagagga
    18301 gtatgtctgc gataaacttg gcatccgtgc ccaagaaatc tctacacaag tccttcctcg
    18361 tgaccttcac gctgagtact ttgcggttct tgccagcatt gcgacttcaa tcgaacgtat
    18421 ggcgactgag attcgtggtc tacaaaaatc tgagcaacgc gaagtagaag agttctttgc
    18481 taaagggcaa aaagggtctt cagcaatgcc tcacaaacgc aacccaatcg gttctgaaaa
    18541 tatgactggt ctggcgcgtg tcattcgtgg tcacatgatt acggcttatg aaaacgtcgc
    18601 tctctggcac gaacgcgata tttctcactc atcagctgag cgtatcatca caccagatac
    18661 gaccattttg attgactaca tgctcaaccg ttttggaaat atcgtcaaga acttgacagt
    18721 cttcccagaa aatatgatcc gaaacatgaa ctcgactttt ggtcttatct ttagccaacg
    18781 ggctatgttg acattgattg aaaaaggcat gacccgtgag caagcctatg acttggtgca
    18841 accaaaaaca gcctactctt gggacaacca agtagacttt aaaccacttc ttgaggcaga
    18901 ttcagaagta acatcacgtc tcacacaaga agaaatcgat gaaatcttca acccagttta
    18961 ttacaccaaa cgagtggatg atatctttga acgtcttgga ctaggtgatt aattaaaaaa
    19021 taaacagcga gcttcaatct cgctgtttat tttttatcga aaagacttag tcttcttttc
    19081 ttttagtgag tccataggct gctagtgtgg acatgagtcc tgcgactact agtcctgcag
    19141 aatcgtgagt tcctgtttca ggaagttttt tctctgttac cacaggagct ggatcttgag
    19201 gaagaacttt gctttcctca gcaggagcag ttgatggagc tggttggctt gggatttcta
    19261 gttttggttt ttcttcagca atagcggctt gtccgttttc atcgcctaca tgtgttacca
    19321 tagttccgac ttcgactatt tgagtaacgg cttcctgtgc tacgacacta tttacaagtg
    19381 ttttcacttc cttaccatcg gcagaagtgc tcacagagta gaagttgcta cgatgtccat
    19441 tgacgccctt agtaatgact tgtgtttttc ctttgagtaa gagtggattt tcacgagtca
    19501 ctgtggtaaa tggaatgtct tcttcttgga tatccagtct aggttttacc tcagtagttg
    19561 gtgcaagacc actttcatca cccttgtgag ttacaggagc gccaacttca accacttggt
    19621 ttataacttc tttggttacc tggctatcaa ggactgtttc tgttgttttt ccattttcag
    19681 tgagtacaga gatgtaatga gttcgttcac ctttgactcc tgctgtgata atattttcct
    19741 gaccggctgg gaggttagga ttttctttct tgataacttc aaatggaatt tcttcagttc
    19801 ttgtgatgag ttctggtctg gtttcaacat tggcagccac ttcattttca tctaggcttc
    19861 ctgaatgagt tacagctggt ttgaggcctt gaagagcggc ttttaggttg gctacaagtg
    19921 tgtcaagctc agcttgttta ttacggttga ggttgtaatt tagagctgtt ttagctgcgt
    19981 caagggcctc aagactttct ttactatatc cttctaagtt tgtaggaatt ttagctaatt
    20041 cttcgcggag agcattataa ttagcacgga agtagtcttt gttgtggtct gcaaaggcag
    20101 tcatgagttc aaagatttcc tcttccttgt attcagcgct tggtctatct gcccagattg
    20161 aaagcatact tccgactgtt ggaagatcta cttcaggata tttggtagaa gctagttgat
    20221 tgaatggtgt ttttccagta ttctcaatag ctttcttgag gaaaccacca ccatcttctg
    20281 gtttttgacc aagaatgtag taccagtcac cgttggtatt caagaattta tagcctttgc
    20341 ttgctaggta ttgaggtgat gcgaggttat atccccacca gcctttagac cagtaagaaa
    20401 tcaagacatc tttgtcaaac tgaacatcgt ccttgtcttc atagtagaag ccatcgttga
    20461 aggccattgg ttgaagccct ctttctttgg ccatagctgc gagggtgttg gcatattcgg
    20521 caaatttgcc atagagttga taccacttga ggtagtacca gccttgggca ctagtcgcat
    20581 cgttggcgta ttcgtcagta ccaaagttga aaatctttgt tttacctgca aagaagtcca
    20641 tgtatttacc gatgagggct tttacaaagt tcatcgcttc ttcgtttttc aagtccatag
    20701 ttgtttttga aactttatca aagtgggctt gaggattttt aatacctaat ttttccatgg
    20761 caaccagcat agcatccatg tgacctggac tgttaatagc tgggatgaga ccgatgtcct
    20821 tagatttagc gtattcaatt agctctgtca cttctgcctg tgttagtgca gtaccgtttg
    20881 gatcgtcgta gtaagcttta gttccttcga taatagcttt tttaacgtca tcactagcat
    20941 aggtttttcc gttggcagta atggtcatat catcgagtag aaagcgaagt ccgtcatttc
    21001 ctagaaggag atggacatca gaatatccga gctcactggc cttgtctacg atgcgtttga
    21061 gctggttcag agtaaagtat ttgcgtccag catcgattga gattatcttg tttttggcaa
    21121 gtttttcaac ctcacgttta gcttcttctt ctttttgagc ttcaggcgtg agggtcaagt
    21181 tgttgacagt ttcttgaagt ttagcaatgg cttgatcaat cgtatcttgt tgggcacggc
    21241 taaggttgct atcgagagag cgaatagctt tttcagcttc ttttacggcc gtgacgcttt
    21301 ctgcagtata acggttcagg tcttttggta cctcgttaag tgcttgctct gcagattcat
    21361 aatcagctgc gaagtattca gcgttggcat ttgcaaaatg acgcatgagt ttgaagaggc
    21421 gtgatggtga ataacgtgca gatggagtgt cagcccaagc agctaccata ccaccgatga
    21481 ttgggatatc agctccttct gtttttggta cagaagtgat tggtgtgttt ttaataccat
    21541 tgagcccctg atcgagattg taccagcctt ggccatcagc gtttcgtcca agaacgtagt
    21601 accaagcatc attggtatta aggatttggt gacctttttc agctagtagt ttagaagaag
    21661 cgacatcgta gcctccccaa ccaccagtcc acatagaaac gatgatgtct ttgtcaaaac
    21721 taccaaagct tgtgtcgcta ttgtagtaga taccgtcgtt aaaggccatt ggtttgagac
    21781 cgtgcgattt tacaatacga gcgaggtcat tggcgtaggc aataaatttt tcatagcctt
    21841 ttacagggta gccttcgttt ggatagtatt tatcagcttg aagcacactc caacctttag
    21901 catctgtcgc atcattggca tattcatcaa gtccgatgtt gaagatttca gtctttttcg
    21961 cgaaataagc agcatacttg tcgataaggg cttttgtaaa agcgacagct tgttcgttgt
    22021 caagatcgac agtacgggct gattccttcc caaaatagct aaagttaggg ttttggattc
    22081 ccaattcttt catggcattg agaatcgcat ccatgtgccc aggactattt actgtcggaa
    22141 tgagaccgat acctttatct ttggcatagt taatcagatc tgtcatttga ctttctgtta
    22201 agtgattgcc gtttggatcg tcgtaataat catttgtacc tttttcaatg gcgcgtttga
    22261 catcgtcact ggcataggtc ttgccgttag ctgtgatgct catatcgtcc aacatgaaac
    22321 ggagtccatc atttccgact aataggtgta aatcagtgta gccataatgt ttcgctttat
    22381 cgatgatttc cttgagctgt tctggtgaga aatatttacg tccagcatca atagaaacaa
    22441 ttttcttttt cgctagtttt tcatttacag ttgcagcacg ttcctttcct gcctctgttt
    22501 ccggtttgtc agcctctgct ttcgcttcat cttttttagc tggtttatcc ttgtcagtct
    22561 tgtctgtatt tgactcttta gaatcaacct ctttcgcttc ttccttttta gggctagctt
    22621 cttctgcctt tttattagca gtttcttttt cagcagaagt tggagttacc acgtctgctt
    22681 tatcactagg agttgaacta acttcctctt gtggtttttc ttctgttttt ggaagactag
    22741 ctaccttatc agtagctgga gtttctgttt ctacagtttt tggagcttct ggttgaagct
    22801 ctgctttagg tgtttcctca gtccgatttt cggatgattg aggggaatca gaaaccgtat
    22861 ggatggtcgg ttggttttct gtagtagtag gagtaactcc atcggctgca acagtctgtg
    22921 cttggaaggc aaatccaatt agaacagaag ctgctcctac agcgtattta cgaatagaaa
    22981 aacgctgttg tttttcatgt ttcattgcaa aacctcctga ttgcattgtt atattgatag
    23041 cgattatata aatcaacgcc tttattttat ttcttatatt aacgagagtc aagaggagat
    23101 gacaaaaaac tataataagt ataaaaaaat ataaaattta aacttaagat ttcagattgg
    23161 tcgaaaatac atatatatat ctagtataat ttttggttct atttctataa aatattccac
    23221 aaattataga atcttccaaa aataggtaag cgctaccttt ttggtgtggt ataataagca
    23281 tagaaaaagc ccgagcgatt aggctcaggc tttcttctta gtgatcacgg tcacatgaga
    23341 tgaattcaat cttgtagtaa tcagatcgtt tgtaagtttc actgtattct aaaacttggc
    23401 cagttgattc gagtttggtg attttagttt gtaggacagt agggaattgt tcatcgactc
    23461 cgaggactga agccgcatgt tctggagttg gaaagactat ttcgttgatt tcttcaaagt
    23521 gttcatcatt catgtgaatg tggtagtcta acttgaaacg attatagata gaactatagt
    23581 attcaaggtt tggataattt gcgttgatat attgttctgg gatgtaggat gtatggtaga
    23641 tataaacgac accgtttgat tcgcggatac gttcaatctt gtagtagaat tgatcgccgc
    23701 gtagacccaa tttttccaag taaacaagct tgtttccgcg ttcaattgaa agaacagtta
    23761 ccttatcatc tttagcattg aagagttcaa tatctgaaaa ctctacaagc ttgtgtttgc
    23821 gtgcacgtga aacgaaggtt ccttttcctt gttggcggac aatatagcca tctttggcaa
    23881 ggtcgtttaa ggcgcgaaca actgtgatag agctgacatc gtacattgaa atgagttctg
    23941 cttcagtgta aaatttatct ccactgctaa actgcccaga gatgatttta ttttttaatt
    24001 cgtcttttat gtattgatat ttgggaattg ccatattttt acctctattt tccttctcca
    24061 tagaaaattt taccataaaa gcgaaaaaaa tagtagtctt
//
LOCUS       DAVLXF010000024        21650 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLXF010000024 DAVLXF010000000
VERSION     DAVLXF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.024"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..2361)
                     /locus_tag="VYO12_000886"
     CDS             complement(<1..2361)
                     /locus_tag="VYO12_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEV1587526.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSKN
                     NLTVTSSGQDISKKYADEVESHLQSILKDVNKNLKKVQHTQNVDFNKKLSRIKTKYLY
                     GLKEKSEAELTLKTKETKEELTAAFEQFKKDTLKSGKKVAEAEKKAKAQKEEDRRNYP
                     TNTYKTIELEIAEAEVGVAKAELELEFAQAQVQIPQDTEKINAAKSKVEAAKSNVKKL
                     EKIKSDIEKTYLYKLDNSTKETPKPRVRRNSPEIKAKGRVKNYEEANIELSKYMTDLY
                     KLDNSTKETPKSRVRRNSPQVGDSRELKETIDKAKETLSTYMVTRLTKLDPSVFWFAD
                     LLMDAKKVVEEYKTKLEDASDKKSVEDLRKEAEGKIESLIVTHQNREKENQPAPQPGG
                     QAGGSMVVPPVTQTPPSTSQSPGQKATEAEKKKLQDLIRQFQEALNKLDDETKTVPDG
                     AKLTGEAGKAYNETRTYAKEVVDKSKKLLSQTAVTMDELAMQLTKLNDAMSKLKEAKA
                     KLVPEVKPQPENPEPKPQPEGEKPSVPDINQEKEKAKLAIATYMSKILDDIKKHHLKK
                     EKHHQIVALIKDLDKLKKQALSEIDNVNTKVEIENTVHKVFADMDTVVTKFQKGLIQN
                     TPQVPEAPKSPEVPKVSDTPKAPDTPQVPEAPKSPEVPKVPEAPKAPDTPQVPEAPKS
                     PEVPKVPDTPKAPDTPQVPEAPKSPEVPKVPDTPKAPDTPQVPEAPKAPDTPQIPEAP
                     APETPAPAPEAPKTGWKQENGMWYFYNTDGSMATGWLEYNGSWYYLN"
     gene            complement(2440..2967)
                     /locus_tag="VYO12_000887"
     CDS             complement(2440..2967)
                     /locus_tag="VYO12_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1587527.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            complement(3050..4381)
                     /gene="hk06"
                     /locus_tag="VYO12_000888"
     CDS             complement(3050..4381)
                     /gene="hk06"
                     /locus_tag="VYO12_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1587528.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIYE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            complement(4378..5031)
                     /gene="rr06"
                     /locus_tag="VYO12_000889"
     CDS             complement(4378..5031)
                     /gene="rr06"
                     /locus_tag="VYO12_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1587529.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDEITQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            complement(5352..7784)
                     /locus_tag="VYO12_000890"
     CDS             complement(5352..7784)
                     /locus_tag="VYO12_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1587530.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(7786..8244)
                     /locus_tag="VYO12_000891"
     CDS             complement(7786..8244)
                     /locus_tag="VYO12_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1587531.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            complement(8367..9092)
                     /locus_tag="VYO12_000892"
     CDS             complement(8367..9092)
                     /locus_tag="VYO12_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587532.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLE"
     gene            complement(9092..10099)
                     /locus_tag="VYO12_000893"
     CDS             complement(9092..10099)
                     /locus_tag="VYO12_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1587533.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(10138..10896)
                     /locus_tag="VYO12_000894"
     CDS             complement(10138..10896)
                     /locus_tag="VYO12_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587534.1"
                     /translation="MRNLRSILRRHISLLGFLGVLLIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            complement(10859..11149)
                     /locus_tag="VYO12_000895"
     CDS             complement(10859..11149)
                     /locus_tag="VYO12_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1587535.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(11220..11322)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(11405..12751)
                     /gene="cbpD"
                     /locus_tag="VYO12_000896"
     CDS             complement(11405..12751)
                     /gene="cbpD"
                     /locus_tag="VYO12_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1587536.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(12840..13106)
                     /locus_tag="VYO12_000897"
     CDS             complement(12840..13106)
                     /locus_tag="VYO12_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587537.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(13108..14460)
                     /gene="dnaB"
                     /locus_tag="VYO12_000898"
     CDS             complement(13108..14460)
                     /gene="dnaB"
                     /locus_tag="VYO12_000898"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1587538.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(14503..14955)
                     /gene="rplI"
                     /locus_tag="VYO12_000899"
     CDS             complement(14503..14955)
                     /gene="rplI"
                     /locus_tag="VYO12_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1587539.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(14952..16925)
                     /locus_tag="VYO12_000900"
     CDS             complement(14952..16925)
                     /locus_tag="VYO12_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1587540.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQIIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(17061..17609)
                     /gene="raiA"
                     /locus_tag="VYO12_000901"
     CDS             complement(17061..17609)
                     /gene="raiA"
                     /locus_tag="VYO12_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1587541.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(17689..18351)
                     /locus_tag="VYO12_000902"
     CDS             complement(17689..18351)
                     /locus_tag="VYO12_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1587542.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(18348..19646)
                     /locus_tag="VYO12_000903"
     CDS             complement(18348..19646)
                     /locus_tag="VYO12_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1587543.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            19702..20337
                     /locus_tag="VYO12_000904"
     CDS             19702..20337
                     /locus_tag="VYO12_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1587544.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            20622..21542
                     /gene="cysK"
                     /locus_tag="VYO12_000905"
     CDS             20622..21542
                     /gene="cysK"
                     /locus_tag="VYO12_000905"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1587545.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLVLAPDNGERYLSTALYEL"
BASE COUNT         6259 a         4907 c         3617 g         6867 t
ORIGIN      
        1 gttgagatag taccatgagc cattgtattc tagccagcct gttgccattg aaccatcagt
       61 attgtagaag taccacatac cgttttcttg tttccagcct gtttttggag cttctggagc
      121 tggagccgga gtttctggag ctggtgcttc cggaatttgc ggtgtgtctg gagcctttgg
      181 tgcttctgga acctgcggtg tgtccggagc ctttggtgta tctggaacct ttggtacctc
      241 tgggctcttt ggtgcttctg gaacttgcgg tgtgtccgga gcctttggtg tatctggaac
      301 ctttggtacc tctgggctct ttggtgcttc cggaacttgc ggtgtgtctg gagcctttgg
      361 tgcttctgga acctttggta cctctgggct ctttggtgct tctggaacct gcggtgtgtc
      421 cggagccttt ggtgtatctg aaacctttgg tacctctggg ctctttggtg cttctggaac
      481 ctgcggtgtg ttctgaatta agcctttttg gaatttagta acaaccgtat ccatgtctgc
      541 aaataccttg tggactgtat tctcaatttc tactttggta tttacattat caatttcaga
      601 aagtgcttgc tttttaagtt tatcaaggtc cttaataaga gcaacaatct gatgatgttt
      661 ttctttcttc agatgatgtt tctttatatc atctaaaatc ttgctcatgt atgttgctat
      721 agcaagttta gctttttctt tctcctgatt aatatctggt acgcttggtt tctcaccctc
      781 tggttgtggt tttggctctg ggttttccgg ctgtggttta acctctggta ccaatttcgc
      841 tttagcttct ttcaatttag acatggcatc gttcaatttg gttaattgca ttgccaattc
      901 atccattgtc actgctgtct gtgatagaag cttcttgctt ttgtcaacaa cttctttcgc
      961 ataagttcta gtctcattat aggcttttcc agcttctcct gtgagtttag ccccatctgg
     1021 aacagtcttt gtttcatcgt ctagtttgtt caaggcttct tggaattgac gaatcaagtc
     1081 ttgtaacttt ttcttttcag cttcggtcgc cttttgtcct ggactttggg aagttgatgg
     1141 aggtgtttgc gtcaccggtg gtacaaccat tgaaccacct gcttgtcctc ctggttgggg
     1201 tgctggttgg ttttcttttt ctctattttg gtgagtcacg ataagagact ctatttttcc
     1261 ttctgcttcc tttcgcaagt cttctaccga ttttttatct gaagcatcct ctaattttgt
     1321 cttgtattct tccacaacct tcttagcatc cataagaaga tctgcaaacc aaaaaacaga
     1381 tggatccagc ttcgttaaac gagttaccat ataggtagac agagtttctt tcgctttgtc
     1441 tatcgtttcc ttaagttctc tcgaatcgcc tacttgcgga gaatttcttc gcactctaga
     1501 ttttggcgtt tctttggttg agttatctaa tttatacaaa tcagtcatat atttagaaag
     1561 ttcaatatta gcctcttcat aatttttgac tctgccctta gcttttattt ccggagaatt
     1621 tcttcgcact ctaggttttg gcgtttcttt ggttgagtta tctaatttat acaaatacgt
     1681 tttttcaata tctgatttaa ttttttctag ttttttaaca ttacttttag cagcttctac
     1741 tttagattta gcagcattaa ttttctcagt atcttgaggt atttgaactt gagcttgtgc
     1801 aaactcaagc tcaagctctg ccttggcgac ccctacttct gcttccgcaa tttccagttc
     1861 gattgttttg taagtattgg ttgggtagtt acggcgatct tcttcttttt gagctttggc
     1921 ttttttctca gcttctgcta ccttttttcc tgatttcaat gtatcttttt taaactgctc
     1981 aaaagctgcg gttaactctt cttttgtttc ttttgttttt aacgtcaact cagcttccga
     2041 cttctctttt aatccataca aatacttcgt tttaattctg ctcaactttt tgttgaagtc
     2101 gacattttgg gtatgttgaa cttttttcaa atttttattg acatccttca atatactttg
     2161 tagatgcgac tcgacttcat cagcatactt cttcgatata tcttgcccac tagatgtaac
     2221 cgtgaggtta ttcttactcc taaccccttc tgcatggact actcctccta agaacaagct
     2281 ggcaactact acactagcta ctccaatact aaatttacga attgaataat gtacttttct
     2341 ttcgcttttt gatgcaaaca tgtttatttc cttctatatt ttttctttaa ccttactata
     2401 tcacttcaat atgaactgta tatgagtcga attttgtatt tatctcttta tgcttccatt
     2461 tgggattata acttcatgta atagcttttc ctcttcagct attcgtcgat acaaaataac
     2521 tgcataaact ggaaaaacta aaacaaaagt catataggca tgactcaaca aggtcaagcc
     2581 aatcaactca ggtaaaatat ttaaaaagta attagggtgt tttactgtcc taaacaagat
     2641 atgatctaca tatttgtgat tattgacaag cataagcttc actgtccaaa tatctcccaa
     2701 caagtgaatc accaacatca acatcagcat agaaaaaata agcaagacta aaccaaccat
     2761 gcccatgcca tcaaaaattg tcttgtgcac cattgcctca accaagcaag agagataaaa
     2821 aacaatatga gccatagtta atcgttttgt attctcaact ccaaattctt tcccgccatt
     2881 gctaaggatt cgtttctcat tctctatcga acgctttaaa aaaatcaatc gaataaagaa
     2941 tgttactaaa ataatcgcta ataccatatt agtatccttt cttttatcta cacaaagaat
     3001 aacacactta tgttaaccct atatgaactt taataaaaaa ctaatctgtc tacaagctaa
     3061 tcttaaattc cataccgtgt tcataaggga gaaaactata atccatttga tagctttcta
     3121 aaatattatt cacaatgtaa agacccaaac cggaactttc atccttatct gtcacgattt
     3181 gagaatgata aaatatatca aataaatgtt ctagttcttg gtcacttaga ggcgcacatg
     3241 tatttttgat agagagatag ccatcttgct cggatatgat tacgcgccca tttttatctg
     3301 aatatttaat tgcattacta atcaggtttg tcaaaacctt atccaatgcc ctaagactca
     3361 tgacgactct ggtagcatct gtcaattgat tttcaattgt aacacctatt gaatgagcta
     3421 attcttgata acgtgataaa atatctacta aaactggctt aacagtcaaa gtctcacgac
     3481 actctgtcca ctcttggaat ttagaagact ccagtacttc ttccaataag tggctcatct
     3541 ggtcaatctt atttatactc tttgcaatat attttggatg atctttgtaa tctccaatat
     3601 tatgctgcat attctctagg ataattctaa gactggctaa aggggttttc aactcatgtg
     3661 atgctccgcg gacaaaggaa accttttgat tttgcaattt tacaatttgc tcattacgac
     3721 tttccaactc ataaataacc ttcaacaagt gctcatacat accattaatc tgttttccaa
     3781 cttcaccaac ttcatctttc ctactttcat caaaacgaat attgtcatcc aaatcttgca
     3841 ttttactagt cacttctgaa atgtaaaaaa gaggattgag caagcgttta gtataaaaat
     3901 aagaaaaaac aaaggaaaaa gcaattgtaa ccaaaaatgt atatgggaga tacaaaagca
     3961 aaatattctt tgcttctttg tagacatcca ccccgtgaac aaattgcact gttacctgtt
     4021 taccatctgc tgtactaaca gacatatcta acatcacgat ataatttgat agctttccat
     4081 ctttaatatc caagtcatgc acaagaggga gccgcttatc tacaatatct cttttaaggt
     4141 gagcagatat ggtcaaggac tttgaataag actggagtaa gcttggaatt tcatcagatg
     4201 tcttagtctt taaatactcc gtaaacactc ttgcgctctc attaaacttt tccccctcca
     4261 gttgaatata ataaaaagga aaggtcaaat aaatagctat atgaatgact agaccaacac
     4321 cacctagaat tgcaaaactt cttaaaaagg acttggttaa taatttagga ttttttatca
     4381 taaactaatc ttatacccaa catttttcac agtcacgata caatctaaag ctaatttttt
     4441 gcgaagagta cgaatataga catcaacgac cctatcacaa ggtaaatcag ctacatcttt
     4501 tgaaatttct tccaatatct gctctctact taaaacttga tttttatgct gaatcaaaca
     4561 ctttagtacc agtaattcct ttggtttgag atcaatttct tcatttttat aatgtgcttt
     4621 gtaagaggta aaatccactg ttacatcctg atatcgccaa atatcctcta tgacgtagta
     4681 acgtctgata agcgccttaa tacgcttgac aagcagtacc aaagaaaaag gttttgtcac
     4741 atgatcatca gcatagagat caaatacctg tgactgagta atttcatctc ccagagcact
     4801 caagacgatg acaggaatat cgtgcttttg attgatttct tttaacactt caaaaccact
     4861 cttcctaggc atcattaaat ccagtaccat gagatggata gggagatctt gaaatttttc
     4921 caagacctct tgtccatcct tagccataat gacatgataa ccctcttctg tcagaaatgc
     4981 tgcaattcct tctctaatca tttcctcgtc atctgcaact aaaatgttca tctctctccc
     5041 tttctactac cagaactcca tacatctatt tcctgtatgc tatttgaaaa tttcctacca
     5101 gcctatcatc tatgataata gaatggattt catttatccg ctactttttg tcaacaacaa
     5161 cgtcaataca agaaaatcat tcatcttgca gcttatactc ttcgaaaatc tcttcaaacc
     5221 acgtcagctc tatctgcaac ctcaaaacag tgttttgagc aacctgcggc tagctgccta
     5281 gtttgctctt tgattttcat tgagtttcac tccactgtga cttatggtca gatgctggta
     5341 cttttattct tttatgcaat atcaaatttt aactggcctg ctttgacacc aatcttaagt
     5401 gtgctgcctg ccactaaatc tcccttgaga agaagttctg ccaacttgtc ctccacttct
     5461 gtttgcaggg ttctgcgaag tgggcgagct cccatctctg ggtcatatcc ttgatttgct
     5521 aacaatttca gagctgaagc ttgtaatttc aagtcaatgc ctttttcagc caaacttgcc
     5581 actaaaggct tgaccataat cttcaccact tcctgcatat gatcactaga taggctatgg
     5641 aagaccacct tctcatcaat acggttgatg aattccggtc tataagcttt tttcagttct
     5701 tcaaacatgc gtttttccat attttcctgg tcaaaacgaa tatccttagc cccaaaacca
     5761 acagtcttat catcacgaag ggcagtcgca cctaggttcg atgtcataat gataatggta
     5821 tttgaaaaat cgaccttgcg tcccttgcta tctgtcaaga caccgtcatc cagaacctgc
     5881 aagagaacat taaagatatc tgggtgagcc ttctctacct catcaaagag gagaacggaa
     5941 tagggtttat tgcgaacctt ctctgtcaac tccccacctt cttcgtaacc cacatagcct
     6001 ggaggagctc catttagacg gctggctgcg aatttctcca tatactcact catatcaaag
     6061 cggataaggg ctgattcgtc atcaaagaga acttctgcca aagccttggc caactcggtc
     6121 ttaccgacac ctgtgggtcc taggaacata aaggaaccga tcggacgctt gtgactgcga
     6181 attcctgact gattacggcg aatcgcacga ctaatacttg aaacagcttg atcttgaccg
     6241 ataacccgtt tatggagttc tgcttcaaga tttagatatt tcttagcgtc agtctgagtc
     6301 agcttttgaa ctgggattcc tgacaagcga ctcaaggtgg tcaaaatatc agactctgtc
     6361 accaagtctt tgtagacagg tacttcctct tcttttgcga ttagctgggc tgcctgtttc
     6421 cacttgccat ccatcagggc cttgtcagct ggactcaaat ctgaatcgtc tgcttttaca
     6481 tgctttgcct tattttgcac tgttgctgct gcttcgtcca agaggtcgat agcagagtct
     6541 ggcaagtgac gactggttaa ataacgatga gccatcttaa ccgctgtttc aaccgcttca
     6601 tctgtgattt gtacacggtg atgtttctca taagtcgcct tcaaaccttg taaaatagtc
     6661 atactatctg ccacacttgg ttcttcaatc gtcactttag cgaaacgacg agaaagtgcc
     6721 gcatcttttt cgatatgttt ttgatattct tcctgagtag tggcaccaac cgttctcaaa
     6781 gttccacgcg ccaaggctgg tttcaagata ttggccgcat ccagagtcga atcaatcccg
     6841 ctaccagaac ccatgatggt gtggagttca tcgataaaga ggatgacttg gccatcttct
     6901 tcaatatcct tgatgatatt attcatgcgt tcttcaaagt caccacggaa gcgtgtccct
     6961 gcaacgacat tcatcaaatc aagttctaac acgcgcatct tagccatttc cgcaggcacg
     7021 tcaccactag caatacgctg ggcaagacca agggccagag ctgttttccc gacaccagca
     7081 tccccaacca agacagggtt gttcttagtc ttacggctca agatttgaat catacgtgag
     7141 atttccttgt cccgaccgat gactggttct aacttgccag aacgcgcttg ctctgtcaaa
     7201 tcatgcgtat agtcctcgag accaccacta ggagtctgcg gcatgcccat catattggcc
     7261 atagaatttt gcttgtcagc tactgtacga tggcgttggc gtaaagcctt gagatcttca
     7321 cgagtccagc ctgcacgttc ttctaaattt cgacgaaggg cagcaatctt gacctgatct
     7381 ttcttgtctt cataagaaaa accagccctc tccaagatac gagtcgccaa ggcattgcca
     7441 tcatgcaaaa tcgcatagag gacgtgctct gtccctagta ccttagcatg gaccactgac
     7501 gctacatact ctgcttcatc aaaaagaacc tgcaaacgac gggagaacgg caattccgta
     7561 aaggtttcat cctggctata gtccgtttca gtcagttcca aagccacctc ttctaaacgg
     7621 tccatctcat acggataatc atttaaagtt gcccctgcta cactataact gtgattagac
     7681 atggcaatca acaagtgcca cgactctaga taacgagctc caaaatgtcc agcaaccatg
     7741 taggcacttt cgatacattc attcaatgct tttgaatagt tcatcttact tcccttttct
     7801 atctacctct tgtatgacct gacgtagcat gtttgctcga acaactggag cttcttctcc
     7861 taaaacgcga tccaaagcta ctgattctag caaattcatc tcctgcttgg tcatcaattc
     7921 ctgctcaacc aaaagctgga gaatatcctc ataaatttct tgactgactc gctcaccaat
     7981 cgagtaaagc agatcccgga gcatttcatg atgactagaa aactcaatcc gtcctatacg
     8041 aatgtagcct ccgccaccac gcttactttc aaccaagtag cctctacttt ccgtaaagcg
     8101 tgtcttgatc acgtagttaa tctgactagg aacaacctga aaggtatctg ccaactgact
     8161 ccgttgcaac tccacgatac cagattgatc taaaatcgcc ttgatgtagg cctcaatatg
     8221 atccgatgta tttttaaatc tcataacaaa cccacctctc tctttaaacc ttgaccttgt
     8281 ttgactatta taccgaaatt ttctcatttt taaaagaaaa agggcgctgg taaaggataa
     8341 tcttcaccaa ctccctattt ttctacttat tctaagccta attctgccaa gatttgacgt
     8401 ttgtaggcaa tcttttggac ttccttgtcc tcatcttcag accaatctag tttaatttct
     8461 gaaacaatct gcccagggcg atttttcaag atatagatgc ggtcgctgag attgagggcc
     8521 tcctcaatac tatgcgtgat gatcagagtt gttagctgca actgtttgtg aatctcaaga
     8581 taccaagcgt ggagttccat ctttgtcatc tcatccaagg cgctaaaggc ctcatctaag
     8641 agaaagagct tgtgcccaaa aaggtaggtc cggagtaagg ctacacgctg gcgcatccca
     8701 ccgctaagtt catgaggata cttgtctctt acagctgtca gctggaaggt cgcaagaatt
     8761 ttatccgctc gggaaatagc ttctgcctta tccacctttt gaatcaagag gggcagaatg
     8821 atatttccaa gcaccgtctt gtgctccaag agcagatcct tttgcaacat ataactcacg
     8881 cgccccttgg gattttcttc accatcaagg acaattctcc ctgactgaac ttctaaaatc
     8941 ccagcgatta gattaaagag ggtggtcttt ccaacaccac ttgggcctag gatggaaacc
     9001 acttcgcctg aagtcacctg tagattgata tcctctaaaa tcctctcctg accataggca
     9061 taactgacgt gctctagtct aatttctgtc attatttcac aaattcgttg gtgaagcctt
     9121 tgtctgtcaa gtcttcttta aggataccat tttctttatc ccatttgtag aaagcattcc
     9181 agcgagctgc gtcaaattga ccccattttt ccttgtcgct tgcgtattct tttgacaagt
     9241 atttttgaga ttcgatgaca aagtcacgtt tttccttgag ttcaggtgca ttcttgatga
     9301 gaatatctgc agcttcttct ggatgttcca tggcatattg gtagcctttt ttgatggctt
     9361 ggatgacttt gcgagcttct tctttgttat ctttcagata gtcgttgttt gcgatgataa
     9421 ctggtgaata gtagtcaaac tccttgacat agtctttcaa gtacatgaag ttagcatcta
     9481 caccttgaga tttagcaagg ataccatccc aaccgtagta aatccaagca gtatcaaaga
     9541 cgccattggc aatcggtgtg attgagtttg agtcgttatt tggtactttt tcaaccttct
     9601 caaagtctcc accttgagat tctaccaagg ttttcaacat agcaagttca gttgggtcat
     9661 tccatgtccc atatttctta ccaaccaagt cttttggact gcttacatta tcagatttac
     9721 gagagatgat tcctgatgta ttgtgttcaa caatagctgc aacggcagtg attcctgctc
     9781 ctttttccaa tttcttagcc atgtagtctt ggaaatacac tgcaaatggt gcctttccgt
     9841 tgataaccaa gtcagaagaa ctttcttctg gtggcaattt caaatcaaca tccactccag
     9901 cttctttgaa ataacctttt tccttggcaa cataaagccc tgtgtggttg gtatttggtg
     9961 tccagtctag gataaagtca accttcttaa gttctgcctc tttgttgtct ttagaagcag
    10021 ttccttgacc acaggccaca agcacaacag ctacaagagc tgttacaagc gttaaaaaca
    10081 ctttccatgt tttcttcatt ttgatttcct cttttctttt tcagaaacat tctaattcta
    10141 cgaacgtttc catttaatca catatttttc actgatatcg accagcttca tacccaaaag
    10201 actgataatc gacaccagaa taataatggc aaacatggta tcatactgaa acaatttttt
    10261 agactgaatc atataaacac caagaccttc aaaacctccc aaccactcag ataccacagt
    10321 tgtgataaag gcgtaggaga cactgaccct cagacctgca taaaagtaag gcaggctaac
    10381 tgggatttta aaatgccaca ggatttgcca aggcttggcc cgcatcagac taaacaaggt
    10441 cagcatatcc ttgtcgcaat gcctaaaacc gtccaaaata ctaacgatga tgggaaaggt
    10501 tgtcgttaag ataatcaaga caatcttggg caaaatccca taacctagcc acaagaccag
    10561 gataggagct atggcaatgg tcggaatggt ctgaatgacc accatcatag ggtaaatcag
    10621 gtcattgagc caagtcaaac tatccatgag cacagccata agacaggcaa tcaaaactcc
    10681 caaaatcagc cccagtaaag ccactctcaa ggtcgcccag ctatggtgcc agagaaattc
    10741 tctgtcacga acaaagggct ggagaatttc aagaggtgtc ggcaggataa acttggggag
    10801 aagtttaaga aaacctgcta actgccagat taacaagact ccgagaaagc ccaatagact
    10861 aatgtgtcgt ctcagtatac ttctcaagtt tctcatcaat acttaaaatc tctcctacat
    10921 ttattttgac attggcaaag acattgtctg cctcctgccc tgccacttcc agcgcttctt
    10981 ttagaatgcg cataagctca tcaaactccc cttccaagac cgtttcaaat ggtgtcacta
    11041 ccatcgtcac ttcttgagtt tgcagataag caatgacctg atcaataaca gctatccgat
    11101 caatcccctg tgctaggggt aaaacttgca aggcaatgct tgctttcata agaacctcct
    11161 cttctcgttt tcctttttaa aaaaagaaaa acctttacca tacatggaaa aggtgcaaaa
    11221 ttagactaag tatcgttccc tccgctggca ttacccagat caggtgcggt cgaagtttaa
    11281 cacttcctct cagactgtac acagactccc atatattata tgtatatatg atagcgcaaa
    11341 ataagaaaaa tgtcaaggaa actgcttaca gaaaagagca ggaaatttct cctactccaa
    11401 ttttctatac tcgttctcca tcactgttta ctctgtagcc atccaccgtc gtattaacag
    11461 ctaaggcgcc tgaagagtaa gaataatacc acttaccaga gacctggtac cagcctgttt
    11521 tcattgcccc tgaactaccc agataatacc acgagccatt ctcctttaac catcctgtct
    11581 gcatttcacc agataatttc aaataatacc agctagaacc gtctttcagc catcctgttg
    11641 atttagaacc atttgatttg aaatgatacc aactaccatt tattttcttc caaccgacag
    11701 ttgaactatt attggatcca tagctagaga tatttcctga ttgattctga taattttgtg
    11761 aagaagtcac tccctctagc tgtatatagc gacgacttcc attataggca gtataactca
    11821 accacttgta gccgtctttt tcaagtatct gatcataatg aaccttctct ccaggataat
    11881 agtaatcaat cacagttccg ctaactaggg gttcagtttt gatagcagac ttggtcttaa
    11941 aataatgagt tccacctgtt gaagaaagaa cagaattacc tagaggattt ttattcacag
    12001 cctccaattg aacataacga tagcttccat tataggcagt ataactcaac cacttatagc
    12061 cgtctttttc aagtatctga tcataatgaa ccttctcccc aggataatag taatcaatca
    12121 cagttccgct agctaggggt tcagttttaa tagcagactt ggtcttaaaa taatgagttc
    12181 cgcctgttga agctgaggat tgactattcc caacactgcc gccatccaaa tctttaaaat
    12241 gaataaatcc tgtcatcgtg tttgctttta taactcgttt attataggat tctgtataac
    12301 catagttata ttcctcaatc tcaatctgat ctcccattac atttgacacc caggcaacat
    12361 gaccataagt tcctgcagta gaccaagtaa tggaaccaat cgtcggtgta ttatctacac
    12421 gataaccttc ccgacgagca cgatgtcccc attcattcgc atttccataa gctgccggaa
    12481 tttcaaaacc attgacatta ctcaaacgaa aggctacaaa agaagtacac tgacgagaat
    12541 acatgcgcca ctgatcaatc tcctggctcc catttttata ataagcagga taatcatccc
    12601 cacgcgcaat cgatccattt cctccggaat aggcatagac actatcacca gctgctagca
    12661 tcaatcctac tactaaaaaa ggaacaagct ttttaactga catcttcaag taataacttg
    12721 tccctcttgc tataaacggt aaaattttca ttcttcctcc ttgaaaaata atataaagcg
    12781 atgattgcct tcacctaaat tattcggaga aaaaagaaaa agtgagaaaa tttctcactt
    12841 tagtcaagat aatgaatcaa attcttttct gtaagaatat ctgagtaagt aaaggattca
    12901 acgtaaacat taacttgttt atctccttcc acatccccaa actccacaat aaatagagat
    12961 ggataaactt caattagctt acccaatcta tttttttggc gcttacgacc attctccaaa
    13021 gtcatttcta cgacttgtcc ctcatgtgcc ttgatttctt ctttgatttt tttcatcttg
    13081 gctacatctg taaatgcatc tgacatctta tgcctccctc tttgagatac ttgaaaattt
    13141 attgtattct ttttggaaaa tcaattccac tgttccacga gctccactac ggtttttctc
    13201 gataataact tccaccttat tatttgggat accctcctct tcttcaccac cacgttcata
    13261 gtagtcatcg cgatagagaa aagctacgat atcagcgtcc tgctcaatag acccagattc
    13321 acgaatatca gacaagaccg gtctcttgtc ctgacgttgt tctacaccac gagaaagctg
    13381 actcagagcg attactggaa ccttcaattc cttggctagt attttcaact gacgagaaat
    13441 ttcagaaact tcttgttgac gattttctcg accagttccc gtgataagtt gcaaatagtc
    13501 tatcaaaatc aaaccaagat ttccagtttc ttgagccaat ttacgagaac gagaacgaat
    13561 ctctgtaatc cgaatacctg gcgtatcatc gatatagata ctggcgttag ctagattacc
    13621 ctgagcaata gtatattttt gccactcctc atctgtcaat tgccctgtac ggatagaatg
    13681 tgactccact aagccttctg cagctaacat acgatctacc aagctttccg cacccatttc
    13741 gagtgaaaaa atagcaaccg ttttgtccaa cttagtccca atattctgag cgatattcaa
    13801 ggcaaatgct gtcttaccaa ctgctggacg agctgctaag ataatcaact cctcctcatg
    13861 aagtcctgtt gtcatatgat ccaaatcacg ataacctgtc gcaatacctg taatatcggt
    13921 cgtttgttgc gagcgagctt ccagatttcc aaagttgaga ttcaacacat ctcgaatgtt
    13981 cttaaacccg cttcgatttg cattttcact gacatcaatc aacccttttt ctgcctgagc
    14041 aataatttca tcagctggtt gtgacgcttc gtaagcttgg ttgacagact ctgtcaactt
    14101 ggcaattaaa cgacgtagca ttgctttttc tgcaacaatc ttagcataat actccgcatt
    14161 agcagaagtt ggcacagaat taacaatctc aaccaagtaa gacaagccac caatattctg
    14221 taaatcacct tgattatcaa ggatagtacg aaccgttgtt gcatctatgg catcaccacg
    14281 atcggataaa tcgaccatgg cttggaaaat caaacgatgg gcatacttaa aaaagtcccg
    14341 agactcaatg tattctcgca caaaaacaag tttactctca tcaataaaga tagcccctaa
    14401 aacggattgc tcagctaaga tatcttgagg ttgtactcgt aactcttcta cttctgccat
    14461 cagacttccc ttcttttaca atcttgtcaa gaaagtgtaa acttatcctt ctttcacacg
    14521 aagattgatt acacttgtga tatcttgata gattttcact ggcacatcaa tcaaaccaac
    14581 cgctcgaatc ggagcttgta cttgaatatg acgtttatca atcttaattc caaattgctt
    14641 ttgcaattct tctgcaatct tcttattggt aatagaacca aaggtacgac catctggacc
    14701 aactttttca acaaattcta caacagtttc ttctgcttca agttgtgctt taattgcttt
    14761 tccttctgca atcatctcag cgtgagcttt ttcttccgat ttttgtttac cacgaagttc
    14821 acctacagct tgagcagtcg cttctttggc tagattcttt ttgataagaa agttttgcgc
    14881 ataccctgtt ggtacttcct taatttcgcc ttttttacct tttcctttaa catctgctaa
    14941 aaagattact ttcattcttc tttctccttt tcctttattt catttaatac aatttctgtc
    15001 agtttttcac ctgcttctga caaggttaca tctttaattt gagctgctgc caaattaaag
    15061 tggcctccac cgcctaactc ttccataatc cgttgtacat tcagtttact acgacttcga
    15121 gctgagatag agataaatcc ttgtgtattc ttcgcaagaa caaaactcgc ttcaatacct
    15181 gacatggcta acatggcatc tgctgcctta ctaataacaa ctgtatcata gcatttcgtg
    15241 tccttagcct ctgctattag tacatctgaa cctaatttac acccctgtaa aataagttca
    15301 ttgacctcac gatattcttc aaaatctgtc gcagcgattt cctggatagc aatactatca
    15361 cttccgcgcg ttctgagata gctagcaaca tcaaatgtcc gactagttac tcgcgaggtg
    15421 aaatttttag tatccaacat cataccagcc atcaaaacac ttgcttgcat acgactcaaa
    15481 cgatttttct tagaattctg gaactgaatc aattccgtta ccaactcact ggcactactt
    15541 gcaccacttt cgatataagt aataaccgca ttatctggaa aatcctgatc ccttctatgg
    15601 tggtcaataa caatgatttg ggtaaataaa tcataaaatt cttttgataa tgttaaggct
    15661 gtctttgaat ggtctacaag aatcaacaaa gaacgattgg tcaccatccc cattgcatcc
    15721 ttaacagaca acaacttcgt aactccttct ttttctatga atgaaacagc tcgttcaata
    15781 tctggagaca tttgttcttc atcataaaga gcatagctat tttcaatcac attgctggcg
    15841 aacaactgca tacctacagc agagcccaaa gcatccatgt ctaaattttt gtgaccgact
    15901 acaaaaacct gatctacact ccgaatctta tctgaaatag ctgtcatcat agcgcgcgta
    15961 cgagtccgtg tacgcttgat tgaagcagca gacccaccac caaaataaac tggatttttc
    16021 gtttcgtcgt tttccttaac aaccacctgg tcgccaccac gtacttcagc caagttcaaa
    16081 ttgagcaaag caactttccc tatctcatca tgatttccat cgccataaga aaatcccata
    16141 cttaaggtca agggcaactg tctctgtttc gactcttctc tgaaagcatc aataacagaa
    16201 aatttatcat tcatcaagcc ctcaagcacc gtgtagtcag taaatagata aaatcgatcc
    16261 atacttaccc gacgagaaaa catcatgtat ttttctgaaa actctgatat aaaattagct
    16321 acaaaactat tgatttgact aatatctgac tcagaagttg catcctccaa atcatcataa
    16381 ttatccacag agacaatccc aatcactggt cgacttgtta ccaattcatc tgttatggct
    16441 tgttccctgg atacatctac aaaatacaaa acaccggaag aagcatccat atgaacagca
    16501 taacgcttct caccaagctt ggcataagta gacggatttc ctactgaagc cttgataatc
    16561 gtttgaacag cttctaaatc aaaatcacca tcttccttgg tcaaaatcaa ttcagcatag
    16621 ggattaaacc actcaacctc tccagaagat aaattcaatt tcataacacc tacaggcatc
    16681 tgttccaata gagctgtcaa actttcttcc gcttggtggt ttacatactg tatctgttct
    16741 acatcactcc ttgtataatg cactctcagt ttcttaaata aaaaaacata gcctcctaca
    16801 aaaagaaaca aaattaaaac cgtcaacaga ttattattaa caaaaataat gaaagtggat
    16861 aagactccaa acgcaatcaa tcctactaga ataggaaaaa ttggacttac ataaaatttt
    16921 ttcattcaaa acctcttggc acccattata ccataatacc cctcaaaaag cgacttttta
    16981 aaagtgtaat cagtaattct atcaattata agaaaaaggt agtttacaat tcaataaacc
    17041 tacctttaca catattgaaa ttaagattct ttaacctcta acaaaccaat ttcgccatcc
    17101 tcacgacgat aaatcacatt ggttgtctga tcttcaacat ccacatagat aaagaaatca
    17161 tgccccaata aatccatttg tagaattgct tcttccaaat ccattggttt taaatcaatt
    17221 tgttttgaac gaacaacttt agactggaca atatttgaat cttccaccaa agcatctgta
    17281 aataattgac cagttgctac cttattttta tttttacgct cgatttttgt tttattttta
    17341 cgaatctgac gttcaatttt atcagttaca aggtcaattg aaccatacat atcttgagat
    17401 acatcttctg cgcggagagt aatagatcca agcggaatcg ttacttccac tttagccgtt
    17461 ttttcacgat aaacttttaa gttaattcgg gcatccaact cttgttctgg ttggaagtac
    17521 ttttcgatct tttcgagttt agaaactaca taatcacgaa ttgcttctgt tacttctagg
    17581 ttttcaccac ggatactata tttaatcata tgagtacctt ctttctaaac atttttgttt
    17641 ttatgatttt attataacgc tttcattcta tttttgcaaa ttttttcctc atcttacaag
    17701 ggaaaatgtt tttacatcct tagcaccagc ttcttccaac agtttcttaa cacgatttat
    17761 agttgctcct gtagtataga tatcatctat aagtaggatt tttttaggaa tagtgactcc
    17821 acttttaata aagaaaggaa gttctgtccc caagcgctct gaacgatttt tagaagaact
    17881 ggctctctct tctcttttct ctaataaatc cagatactca aagcctgctg cctctaccaa
    17941 gccctcaacc tgattaaatc ctctgttagc atatctatca ggacttaggg gaattacaac
    18001 aaattgatac tctttgtact ttttcaactc ctcacttaaa aatgaagcga aaacttttct
    18061 taacaggaag tctccatcaa acttataccg actgaaaaaa tccttcatag cttgattgta
    18121 agtaaaaatc gctctatgac tgacttcaac tccttcttta caccaaagtt gacagtcttg
    18181 acactttgtt gacaactctg ttttcataca atttggacag ttctcttccc caattctttc
    18241 aaaagtagaa tcacagtctg aacaaagaca agagtcatca ttcctcagaa gtaagagact
    18301 actaaaagtt aaaacagtct tcatagtctg cccacataac aagcacttca tagaccagcc
    18361 tccttattca tcatctgaat ttccttaatc gccttcttga ttgaagcatt taacccatca
    18421 tggaagaaaa gcaaatctcc tgtcggtcta tccatgcttc gtccaactcg tccaccaatc
    18481 tgaatcaaac tagacttggt aaacaaacga tgattggcct ctactacgaa aacatccaca
    18541 caagggaagg taactccacg ctccaagatt gtcgtactga taagtattgt cagttctcca
    18601 tctcgaaaag cttgtacttg ctctaatcga tcctctgtta cagaagatac aaagccaatt
    18661 ttctcatttg gaaattgctc ctgtaagatt tcttctaact gctccccttt cttaatttct
    18721 gaagcaaaaa tgagtaacgg ataagctgtc tttctctgct tctcaatata ggactttaac
    18781 tttggtgaca aacgattctt gtctaagtag cgattaaaat ccgataacca aattggtttt
    18841 ggaataatca acggatttcc atgaaaccgt ctcggtaaac tcagtctttt tagttctcct
    18901 aaacggacct ttttatctaa ctcattggtc gaagtcgctg ttaaaaagat tctcaatcca
    18961 ttctccttta cactattctt gacagcgtgg taaagcgtgg gattatcaac ataaggaaaa
    19021 gcatctactt catccactat cagcaaatca aaagcttgat aaaacttcaa taactgatgg
    19081 gttgttgcaa caactagtgg tgttcgaaaa taaggttccg attctccatg tagcaaagct
    19141 atcccgcaag aaaaatcctg ttgcaggcgc ttgtacagct ccaaacaaac atctatgcga
    19201 ggactagcca aacacactgc accacccgca ttgatcactt tagccactac ttgataaatc
    19261 atttctgtct ttccagctcc tgttaccgca tgaactaagg ttggcttttg cttgtctact
    19321 gcttgaagca gtccctctga caccttctct tgaaaaggag ttaattggcc gcgccatttg
    19381 agaacatctt gcttcggaaa atcctcctgc ggaaaatagt ataaagtttg atcacttctg
    19441 actcgcttca tcagcaagca ctctcgacaa tagtaagcac cgatgggcaa ataccattct
    19501 tctagaatag tactattaca acgttgacag aaaagtttcc ccttctcctt tctcattgct
    19561 ggaagtttct ccgccaactg acgttcttct tctgttaatt cattctcagt aaataaacga
    19621 ccgagataat ctaaatttac tttcatactt ctttattcgt aaaaactagc actttagatt
    19681 attttttagt acaattaaat catggaattt aggacaatta aagaggacgg ccaagtccaa
    19741 gaagaaatca aaaaatctcg ctttatctgc catgccaagc gtgtttatag cgaagaagag
    19801 gctcgtgact tcattactgc catcaaaaaa gaacactaca aagcgacaca taactgctct
    19861 gcctttatta ttggagaacg tagtgaaatt aaacgtacaa gtgatgatgg tgagcctagt
    19921 ggtactgctg gtgttcccat gcttggggta ctagaaaatc acaatctcac caatgtctgt
    19981 gtggtcgtga cacgctactt tggtggtatt aaactaggcg ctggaggact aattcgtgct
    20041 tacgccggca gtgtcgcctt agctgtcaaa gaaattggta ttattgaaat aaaagaacag
    20101 gctggcattg ctattcaaat gtcttatgct cagtaccaag agtacagtaa cttccttaaa
    20161 gaacatgatc tcatggagct ggatacaaac tttacagatc aagtcgatac gatgatttat
    20221 gttgataaag aagaaaaaga aactattaaa gctgcacttg tggagttttt taatggaaaa
    20281 gtcactttaa ctgaccaagg tttacgagag gttgaagttc ctgtaaactt agtgtaaaca
    20341 atgaataata cagcgtttcg ttgacattct cacaactact ttagcgagca aaataaaaag
    20401 atgcgtacca aaatatacta gaaaatgaag caattcaaac gaaacctgat atcgttttcc
    20461 ttcacaccta tttactagaa ttaactgaac gcaatcactt gaaaattaat gactttgatc
    20521 tatgatatat agaaatccta tcgcttcgat aggatttcta tattttacaa atggtatgga
    20581 tagcgttata ctaaagatat cttatacaaa gaggtattca tatgtctatt tataacaaca
    20641 ttactgaatt aatcggtcaa acaccgattg ttaaacttaa caacatcgtg ccagaaggtg
    20701 ctgcagacgt ctatataaag cttgaagcat ttaatcctgg ttcatctgta aaagaccgta
    20761 ttgcccttag catgattgaa aaagctgaac aagatggtat tctgaaacct ggttctacta
    20821 ttgttgaagc aacaagtgga aacactggta ttggactttc atgggtaggt gctgctaaag
    20881 ggtataaagt cgtcatcgtt atgcctgaaa ctatgagtgt agaacgacgt aaaattatcc
    20941 aagcttatgg tgctgaactc gtcctaactc ctggtagcga gggaatgaaa ggtgctattg
    21001 ctaaggctca agaaatcgct gctgaacgtg atggtttcct tcctcttcaa tttgacaatc
    21061 cagctaatcc agaagtacac gaaagaacaa caggagctga gatactagct gctttcggta
    21121 aagatggatt agatgccttt gttgctggag taggtaccgg tggaacgatt tctggtgttt
    21181 ctcatgcact caaatcagaa aattctaaca ttcaagtttt tgcagtagaa gcagatgaat
    21241 ctgctattct atctggtgaa aaacctggtc ctcacaaaat tcaaggtatc tcagctggat
    21301 ttattcctga tacacttgat actaaagcct atgatggtat cgttcgtgta acatcagatg
    21361 acgctcttgc actcggacgt gaaattggtg gaaaagaagg cttccttgta gggatttcct
    21421 cagctgcagc tatctacgga gccatcgagg ttgccaaaaa attaggtaca ggtaaaaaag
    21481 tccttgtcct agcaccagat aacggtgaac gttatctctc tacagcactt tatgaattgt
    21541 aaccgtccaa taacgaagta tattgaaaaa tctccagact agagaactca cggatagttc
    21601 ctaatctgga gatttcttat ttgcactttt cttgtacaac tttagtccat
//
LOCUS       DAVLXF010000025        21292 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLXF010000025 DAVLXF010000000
VERSION     DAVLXF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.025"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            32..328
                     /locus_tag="VYO12_000906"
     CDS             32..328
                     /locus_tag="VYO12_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1587546.1"
                     /translation="MDTPDENGYVADNYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDLVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGASIG"
     gene            449..1393
                     /gene="manA"
                     /locus_tag="VYO12_000907"
     CDS             449..1393
                     /gene="manA"
                     /locus_tag="VYO12_000907"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1587547.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(1414..2777)
                     /locus_tag="VYO12_000908"
                     /pseudo
     CDS             complement(1414..2777)
                     /locus_tag="VYO12_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3005..3754
                     /locus_tag="VYO12_000909"
     CDS             3005..3754
                     /locus_tag="VYO12_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1587548.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(3774..4229)
                     /locus_tag="VYO12_000910"
     CDS             complement(3774..4229)
                     /locus_tag="VYO12_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1587549.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4249..5424)
                     /locus_tag="VYO12_000911"
     CDS             complement(4249..5424)
                     /locus_tag="VYO12_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1587550.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDNQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5483..6328)
                     /locus_tag="VYO12_000912"
     CDS             complement(5483..6328)
                     /locus_tag="VYO12_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1587551.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6453..7010
                     /locus_tag="VYO12_000913"
     CDS             6453..7010
                     /locus_tag="VYO12_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1587552.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7029..7496
                     /locus_tag="VYO12_000914"
     CDS             7029..7496
                     /locus_tag="VYO12_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1587553.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7585..8214
                     /gene="upp"
                     /locus_tag="VYO12_000915"
     CDS             7585..8214
                     /gene="upp"
                     /locus_tag="VYO12_000915"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1587554.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8388..8978
                     /gene="clpP"
                     /locus_tag="VYO12_000916"
     CDS             8388..8978
                     /gene="clpP"
                     /locus_tag="VYO12_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1587555.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9057..9305
                     /locus_tag="VYO12_000917"
     CDS             9057..9305
                     /locus_tag="VYO12_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1587556.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9407..10567
                     /locus_tag="VYO12_000918"
     CDS             9407..10567
                     /locus_tag="VYO12_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1587557.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDSLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10835..11704
                     /locus_tag="VYO12_000919"
     CDS             10835..11704
                     /locus_tag="VYO12_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1587558.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11708..12664
                     /locus_tag="VYO12_000920"
     CDS             11708..12664
                     /locus_tag="VYO12_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1587559.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12664..13428
                     /locus_tag="VYO12_000921"
     CDS             12664..13428
                     /locus_tag="VYO12_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587560.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13428..14138
                     /locus_tag="VYO12_000922"
     CDS             13428..14138
                     /locus_tag="VYO12_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587561.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14446..15102
                     /locus_tag="VYO12_000923"
     CDS             14446..15102
                     /locus_tag="VYO12_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1587562.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIISL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15210..16305
                     /gene="prfB"
                     /locus_tag="VYO12_000924"
     CDS             join(15210..15281,15283..16305)
                     /gene="prfB"
                     /locus_tag="VYO12_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1587563.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16323..17015
                     /gene="ftsE"
                     /locus_tag="VYO12_000925"
     CDS             16323..17015
                     /gene="ftsE"
                     /locus_tag="VYO12_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1587564.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17008..17934
                     /locus_tag="VYO12_000926"
     CDS             17008..17934
                     /locus_tag="VYO12_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587565.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            18220..20400
                     /locus_tag="VYO12_000927"
     CDS             18220..20400
                     /locus_tag="VYO12_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1587566.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEV
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     NAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20452..21267
                     /locus_tag="VYO12_000928"
     CDS             20452..21267
                     /locus_tag="VYO12_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1587567.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
BASE COUNT         6523 a         4013 c         4642 g         6114 t
ORIGIN      
        1 tagtattatc gtagaaaatg gactcggtgc tatggataca ccggatgaga atggttatgt
       61 agcagataac tatcggatta cttacttaga ggcccacatc aaggccatgc gagatgccat
      121 ttaccaagac ggggttgact tgcttggtta tacgacttgg ggctgtatcg atctggtttc
      181 agctggcaca ggggaaatga ataagcgcta tggctttatc tatgtagatc gagataatgt
      241 aggaaacgga gctctcaaac gtagcaagaa gaagtccttc tattggtaca aggatgtcat
      301 tgatagcaat ggtgcaagca tcgggtagag cacatttgtt tactattttt ataaaatctt
      361 ctccctgctt tataagatgt aaaaaagagt tcaattcgaa ctcttttttt gctataatga
      421 ggggagaaaa atcagacagg agatcgacat gtcagaacca ttatttttac aatcagttat
      481 gcaagaaaaa atctggggtg gagccaagct acgtgatgag tttggctacg acatcccaag
      541 tgaaaaaatc ggagaatatt gggccatctc agcccatcca aatggagtct ctaaagttgc
      601 caatggtcgt tacgagggaa cagatcttgc tactttgtat gcggaacacc gtgaattatt
      661 tggcaatcgt ccagaacctg tatttccact tttgaccaag atcctcgatg ccaacgactg
      721 gctcagtgtc caagttcacc cagacgatgc ttatggactc gagcatgaag gcgaactcgg
      781 aaaaacagaa tgctggtaca ttatcgcagc ggatgaaggt tcagagatta tctacggtca
      841 caatgccaag tcaaaagaag aactccgcca gcaaatcgag gataagaact gggatgcgct
      901 gttgacaaaa gtgccagtta aagcaggaga tttcttctat gtaccaagcg gtaccatgca
      961 cgctatcggg gcgggtatct tgattcttga aacccagcag tctagcgata ccacttaccg
     1021 tgtctatgac tttgaccgaa aggacgataa gggcaacttg cgtgaacttc accttgaaaa
     1081 atctatcgat gtcttgaaca ttggtgaacc cgcaaatagc cgtccagtga caatcaaagc
     1141 agatgatcta cgttccactc tccttgtatc caatgatttc ttcgcagttt acaaatggga
     1201 aattactgga aaagttaact ttgaaaagac agctgactac agcttgttga gtgtcttggc
     1261 tggtcaaggt caactaactg ttgacgggaa aaactatcca atccaaaaag ggagacactt
     1321 tatcctacca agtgatgttg aatcttggac tttagaaggg caagatttgg aattgattgt
     1381 tagtcatcca taaaaagaaa gggcctgagt tcactactca agtccttttt gattacataa
     1441 attgggcgat gaagaccacg atgatgattg gaatgacgaa acgaagaaac gaaggaggaa
     1501 gagccagact tggaaaagta cttgtttcca ggctctttca tcgagatgga gttcctccat
     1561 aacaagagcc tttttaaaga tatagcctgt aaaaagtgaa aggcagagag ctccaaatgg
     1621 catgagaaga ttggaaacca agaaatccat ggcatcaaag aaagtcttac caaagatgtg
     1681 aacatccgcc ataactccat aggatagggc tgaaggaatg ccaaagacaa aggtcaaaat
     1741 tcctaaaata acactccatt tggcacgttt gctgttatcc tggttggtga tattgcccac
     1801 attgatctcc agcatcacga cagaaaaagt gactggcgca aaaaggaaga gcaagaggaa
     1861 aaggacgtag aaaatggttc caaaaggcat cttgtcaaag agttgaggca agacgataaa
     1921 gagcaagctc ggtccccctt cagactggat attgaagggg gaccgagctt ggaaaatggc
     1981 tagacctgcc atgatggata tcgagatatt catggctacg atggagattc ctgactggac
     2041 taggttggtt ttcttgtcca agtaagaagc ataggtcaac atgactgtaa cccctagtga
     2101 gagggcaaag aaagattgtc ccagagcata gaggagacca gcgctagtca gttttgaaaa
     2161 gtctggtttg aggaagtaaa gaaccccttc catggcattt ggcaaactga gagagcgacc
     2221 gatgataaca acaaagatga taaagagcag gggcatcatg actttcgaag ctctttcaat
     2281 ccctttttga accccacgtg atacaataaa gatatttaat aagataaagg ctgcttgagc
     2341 tcctagggca atggctggat ttgaaatgat tgaagtaaat aactgagcat aatcacccgt
     2401 tccaccaagt tggaacaatt tcccaaactc aatacctaga tagactagaa tccatcctcc
     2461 gataacactg taaaaagata agaggataaa gagggcaaag gcgccaatcc aaccgataaa
     2521 gttgtacttg ttattcttgc ccagttttcc aaaggttttg atagcggaaa cgccagcact
     2581 acggccaagg gcaaactcag ccagcaggag agggaaaccg attaaaatag tggaaatgag
     2641 aaagattagt aaaaagcctc catcgccatt agcagcagtc atgtagggaa acttccaaac
     2701 ggaaccaagc ccgatggcca gccagcagat gctagaataa aaccaagttt cgacccccat
     2761 tgtgattttt ctgacatagt taaactctga tctccttttt caaaataaga aaaagctact
     2821 tgagttgtca aatagccttc tgtttgattg atagacttga ctatcctatc atgttttcta
     2881 aggtctgtca agaaaatcat tttcaagttt tcacaccttt ctcaaaaaag ttaaaaaatt
     2941 ttctcaaaaa cgcttgactc tgacctaagg ggaggggtta tactatcatt gtaaggagga
     3001 aatcatgtac catataaaag aagctgcgca gctttcaggt gtctctgtca agaccctgca
     3061 tcactatgac aagataggac tcttggtccc cttaaagtcg gaaaacggct atcgaaccta
     3121 cagtcaagag gatttggaac gccttcaggt cattctttac tacaaatatc taggcttttc
     3181 tttagagaaa atagcagagc tgttaaagga agaaaggaca gatttattgc cccatttgac
     3241 taggcagttg gactatctaa ctcgcgaaag gcaacatctg gataccttga tttccacctt
     3301 gcaaaaaact attcaagaac aaaaaggaga aagaaaaatg accattgagg aaaaattcac
     3361 gggatttagc tatcaagaca atcaaaaata ccaccaagaa gcggtagaga aatatggtca
     3421 agaagtcatg ggacaagcgc tcgaacgcca aaaaggtcac gaagacgagg ctacggccgc
     3481 ctttaaccaa gtctttcaaa ctttggcaca aaatcttcaa gttggtttac ctgcaacagc
     3541 aaccgaaaac caagagcaag cagccaagct cttgcaagcc attcgcactt atggatttga
     3601 ctgctctatt gaggtattcg gtcatatcgg taaaggttac gtctataacc cagagtttaa
     3661 ggaaaacatt gacaagtttg gttctggaac agcccagtac acgtcagatg ccattgcagc
     3721 ttacgttcag acaaatgcag aaaaaatagg ctaggaattt cctagcctat tttttacttc
     3781 aaatcataaa gccagtcgtc accgtttttg tagtaaaaga attcactgag atcttcttct
     3841 agaaacacac gaagcatatc agacatatca tcggttgcaa gttttagatg agaaagattt
     3901 tcaaagtcct cccaccaaac tttcccttcg tctgaagact ggagttcacc agtaaagtgt
     3961 tctgtcttgt aaaaaaggac gacataacga taatccttgt cgtcatacca gtttttgata
     4021 ccacagagtt ggggtttgga aatgatcaga ccagtttctt ctttcacttc acgaatgaca
     4081 gcatcgacaa aggattcgcc acgttcaaca tgaccaccag gaaaagtaat gccaggccag
     4141 tcgggactaa ctcggtcttg gaccaggacc ttatctccgt ttttaatcat acacatgtta
     4201 acaaattcga ctgcctctct tctgttcatt cttcacaacc tttaatcttt aatcataatg
     4261 cagacttccc gccacccagc cggtacagag ggcagaagtg atgttaaagc cacccgtgtg
     4321 ggcattgata tccataactt cgcctgcaaa gtggaggcca ggtaccagct tactttcaag
     4381 ggttttagga ttgatttcct tgagactgac tccacccttg gtaacaaagg actttgcaag
     4441 ggacattttt ccagttacag gaattttaag ttctttaatg gactggacaa gctgttctcg
     4501 ttccttttca gtcagctgtt tgactttttc aggatatcct tgtacaaaaa attcggccaa
     4561 gcgttctggt aacaaggttt ttaaagcgtt tttcaaggat ttttcccgat tttcttctag
     4621 aaatgtaacc aagtccttct cagaaagttg aggcaaaaca tcgagtgaga gaacctcccc
     4681 acctttgaca aagctagaca tgcgtagggc agcaggacct gacaaaccaa agtgggtaaa
     4741 gagtaaatca tgagtgatga catgcttacc ataacttagg gtcacatcgt ccaaagaaat
     4801 cccttgtaag gctttatgtg gaaaatctgt taataaagga ctttcagcag cctcaagatc
     4861 ggtgatggta tgcttaaaat ggcgagcaat ctcgtgacca aaaccagtcg aaccagtcga
     4921 aggataagac ttaccacctg ttgtgacaat gagtttctca caagtgaagg tttgatccgc
     4981 tgacttaagg acaaactggt tatctacttt tttaacagaa acgatttcta tttgagtagc
     5041 aacttgacca cctagttcgg tgattttctt ttccaaagct tcgataatag tccgagactt
     5101 gtcactggct ggaaagacgc gtccgtggtc ttcgacctta agtttaacac cattttctgt
     5161 aaaaaagttg atgatgtcat gattatcgaa ctgggagaaa acactgtaaa gaaagcgtcc
     5221 gtttccagga attccagcta gcaggttgtc taagctacca ttgttggtca cattgcaacg
     5281 tcccccacca gtcccagcta atttttttcc aagtttccga tttttttcga tgaggagggt
     5341 tttctgtcca taaaagctac tggaaatcgt agccatcata ccagcaggtc ccccaccgat
     5401 gacaatagta tcaaaatgtt tcatagctct attgtaccac aaaaaaacaa gagatgatgg
     5461 tcacctcttg tcaagaatgc aattaatcaa tttcatagcc catcagcaaa ccgccctctt
     5521 ctgcatagaa actgcagaga ccagaggttg gtagaatttt aatatccgct tgtgggaagg
     5581 tttcacggat tcgctctgag agctgttgac aacatttttc gttattgcgt tgggccatga
     5641 caatacggcc accagcatat ccagctttta ctaactcatc ataggcagct tgaactgatt
     5701 tctttgatcc ccttgctttt tgtagcaatt cgagagtccc agtttcacta gcttttccga
     5761 ccatacgaat gttgagaagg ccaacgaccg taccgataag cttgctcaaa cggccgttct
     5821 tcaccaagtt atcgactttg gctaggacaa agagcaactt agttttttct tgataggcgg
     5881 tgatagcttc aaccacttct tcaaaagaca agccctggtc aatcaagtca ttcaattttt
     5941 ctacgagtag gtcaacttca ccaccagcag ataaactatc aatcacatga atcttagtgt
     6001 caggatggtc ttccagataa atattctttg ctagttgagc actattgtga ctgccagaaa
     6061 gggtacctgt gatggttact aggaaaatgt ttttggcacc ttcaaatgct cgcaaatagt
     6121 catctgggct tggacaagct gattttgaag cttctgcagt tgcatacatg gtttccatca
     6181 tttggtcaat atcgagactg gcgtcatcaa caaagacctg atcagctact tgaatggtta
     6241 aggggacact tacaaaggtt gtgttaatag ctggtgttgg cagttgacga taatcacaac
     6301 cagagtcagc aataatcttc caagtcatag aaattctcca tctttgtcag ttatacattg
     6361 acaaaggttc tgtctttttt tacaattata tcatgaaaac tcttgaaaca aaagcatcat
     6421 ccgtctgttg tgacaagtag aaagaaaatg ttatgtctga acgtagaatc tctgaaaagt
     6481 ctcttgaaaa tctcagaaaa tcaaatcaag aatccaattt attaaccaga gaagccattg
     6541 aaacagccct cttgcaactc ttggaaaaaa aggagctgac caagattagt atttctgaat
     6601 tggtcaaacg tgcaggtgtt tcgcgtgcgg ccttttatcg caattatgac tccaaagagg
     6661 aaattttaga gagcgtcttt aaacgaactg tccacaatat tatggaacag ctgcatcatt
     6721 acgatttaaa gacagacctt tatttggttt gggttcacct tttccgggag gccagaaagg
     6781 aagctagagt aattcaattg gccttggatt accatctgga aaaaatcttt gtccaagcca
     6841 tgcaggaatt tctagaaaaa taccatggga aatcaaaagg tgtcagctct tatcttcatt
     6901 ccttctggag ctcagccatc gtctctgtcc ttctaaaatg gatcaaggat ggcatgaagg
     6961 taccagctga aaagattgca gatttaggtt taccattttt taaaaaatag agaaaaagga
     7021 gaaaagagat gactgaaaaa agactggcct gggatgagta ttttgcagcc caagccctac
     7081 taattgcgaa tcgttccact tgtaaacgtg ctaaagtggg cgcgattctg gtaaaagata
     7141 ataaggttat ttccactggt tacaatggtt cggtgtcagg gactgagcat tgtattgatc
     7201 acgaatgttt ggtcattgaa ggccactgtg ttcgcaccct tcacgctgag gtcaatgcta
     7261 tccttcaagg tgctgagcgt ggtgttccca aaggctttac agcctatgta acccattttc
     7321 cttgtctcaa ctgtacaaaa caattgcttc aggtcggctg caagcgcgtg gtttatatca
     7381 accagtaccg aatggacgac tacgcccaat acctttatca agaaaaaggg acagaattga
     7441 cccatttacc acttgagaca gtacagacag ctcttaaaga ggctgatcta atgtaaaaat
     7501 tatcaaaaat aaatggttta gaaagatttt taaaccgttt tttggtataa tgagaagaat
     7561 aaattgaaag aaggaattcc aaaaatggga aaaattgaag ttattaatca cccactgatt
     7621 caacacaaat tgtcaatctt gcgtcgtaca gatacttcta caaaagcttt tcgtgagcta
     7681 gtagatgaga ttgcaatgtt gatggggtat gaagtacttc gtgatcttcc actagaagat
     7741 gtggaaatcg aaacaccaat tacaaaaaca gttcaaaaac aattggcagg taagaaattg
     7801 gccatcgtcc caatcttgcg tgcaggtatc gggatggttg atggtctctt gagcttggtt
     7861 ccagctgcta aagttggcca catcggtatg taccgtgatg aagaaacact tcaaccagtt
     7921 gaatacttgg tgaaattgcc tgaggacatt gaccaacgtc aaatttttgt agtagaccca
     7981 atgttggcaa caggtggctc agcaatcttg gctgttgatt ctcttaaaaa acgtggcgca
     8041 tcaaatatca aatttgtctg ccttgtatct gctccagagg gtgtaaaagc ccttcaagaa
     8101 gctcatccag atgtagaaat ctttacagca gccttggatg aacgtttgaa cgaacacggt
     8161 tatatcgttc caggtcttgg agatgctgga gaccgcttgt tcggtacaaa ataagatcga
     8221 aaagaagaat gacttggaaa agtatttcca gtcacgaaag gaggttgggt ttttgtttct
     8281 gtctaatgaa agcagagcaa aaatttgacc ttttttgacc aagatattat aatagtctta
     8341 tcttcagtca tttgaccaac aaaattaaaa ctcaaaagga gaaatgaatg attcctgtag
     8401 ttattgaaca aacaagccgt ggagaacgtt cttacgatat ttactcacgt cttctcaaag
     8461 accgcatcat tatgctgaca ggtccggttg aagacaatat ggctaactct gttattgccc
     8521 aattgctttt cttggatgcc caagatagta caaaagatat ttacctttat gtcaatacac
     8581 caggtggttc tgtttcagct ggtttggcaa tcgtagatac catgaacttt atcaaggcag
     8641 atgtccaaac cattgttatg ggaatggctg catctatggg aacagtcatc gcatcaagtg
     8701 gagcaaaagg caaacgtttc atgcttccaa atgctgaata catgattcat caaccaatgg
     8761 gcggtacagg tggtggtacc caacaaactg atatggctat cgctgcagaa cacttgctca
     8821 aaactcgtaa taccttggaa aaaatcttgg ctgaaaattc aggtcagtca atggaaaaag
     8881 tccatgcaga tgcagaacgt gataactgga tgagcgccca ggaaacactt gaatatggct
     8941 ttattgatga aattatggcc aacaattcat tgaactaatg atgatagaag gcaaactcga
     9001 ctgggtttgc tttttttggt ataataggga gagatttctt agaaagagga tttatcatgt
     9061 ttgaaaaagt caatcgctct ggcttgatta tctatcttta ctataatcgt gatgccaaaa
     9121 aactgcagga ttatggagat attacctatc attccaagaa acatcgttac ttacaactct
     9181 atgttccaac tcaagaagtg gagcaattgg tcggacgctt gagcaaggaa aaatttatta
     9241 aaaaagttag agtttgtcat atccaagagt tggaaacacc ctttgtgggc aatctttatc
     9301 gataggaaaa cgttatcatc gaaaaagttc aaggaaaaca ttgacaattt tctgataatt
     9361 cggtatattc ttaacagact atttaagaaa taaggagaca aaaaagatga agaaaaaatt
     9421 tgccctatcg tttgtggcgc ttgcaagtgt agcacttctt gcagcctgtg gagaagtgaa
     9481 gtctggagca gtcaacactg ctggtaactc agtagaggaa aagacaatta aaatcgggtt
     9541 taactttgaa gaatcaggtt ctttagctgc atacggaaca gctgaacaaa aaggtgccca
     9601 attggctgtt gatgaaatca atgccgcagg tggtatcgat ggaaaacaaa tcgaagtagt
     9661 cgataaagat aataagtctg aaacagctga ggctgcttca gttacaacta accttgtaac
     9721 ccaatctaaa gtatcagcag tcgtaggacc tgcgacatct ggtgcgactg cagctgcggt
     9781 agcgaacgct acaaaagcag gtgttccctt gatctcacca agtgcgactc aagatggatt
     9841 gactaaaggt caagattacc tctttattgg aactttccaa gatagcttcc aaggaaaaat
     9901 tatctcaaac tatgtttctg aaaaattaaa tgctaagaaa gttgttcttt acactgacaa
     9961 tgccagtgac tatgctaaag ggattgccaa atctttccgc gagtcataca agggtgaaat
    10021 cgttgcagat gaaactttcg tagcaggtga cacagacttc caagcagccc ttacaaaaat
    10081 gaaagggaaa gactttgatg ctatcgttgt tcctggttac tataatgagg ctggtaagat
    10141 tgtaaaccaa gcgcgtggca tgggaattga caaaccaatc gttggtggtg atggattcaa
    10201 cggtgaggag tttgtacaac aagcaactgc tgaaaaagca tcaaacatct actttatctc
    10261 aggcttctca actactgtag aagtttcagc taaagctaaa gccttccttg acgcttaccg
    10321 tgctaagtac aatgaagagc cttcaacatt tgcagccttg gcttatgatt cagttcacct
    10381 tgtagcaaac gcagcaaaag gtgctaaaaa ttcaggtgaa atcaaggata gccttgctaa
    10441 aacaaaagat tttgaaggtg taactggtca aacaagcttc gatgcagacc acaacacagt
    10501 caaaactgct tacatgatga ccatgaacaa tggtaaagtt gaagcagcag aagttgtaaa
    10561 accataatag aaaaatgttg aaatagggaa tgagcctttg actcactccc tgtttcgata
    10621 tttaatactc ttcgaaaatc tcttcaaact gcgtcaacgt cgccttggat tatatatgtg
    10681 actgacttcg tcagtcttat ctacaacctc aaagcagtgc tttgagcaac ctgcggctag
    10741 tttcctagtt tgctctttga ttttcattga gtataagaac ctatcaaaaa gtgagggaaa
    10801 accctcggaa ttataaatag aaagagtgaa tcttatgctc caacaactcg taaatggttt
    10861 gattctaggt agtgtttacg cgctgttagc cctaggatat accatggttt acggaattat
    10921 caagctcatc aacttcgccc atggtgatat ttatatgatg ggagccttta tcggttattt
    10981 cttgatcaat tctttccaaa tgaatttctt tgtagcgctt attgtagcta tgctagcgac
    11041 agctattctt ggtgtcgtga ttgagtttct tgcttaccga cctttgcgtc actctactcg
    11101 tattgctgtt ttgattacgg ctattggggt ttctttccta ttggagtatg gaatggtcta
    11161 tctggttggt gccaataccc gtgccttccc tcaagcgatt caaacagttc gatatgattt
    11221 gggaccaatt agcttaacaa atgtgcagtt aatgattttg gccatttcct tgattttgat
    11281 gattttgtta caagtcattg tccaaaagac taagatgggg aaagccatgc gtgcagtatc
    11341 agtagatagc gacgcggcgc aattgatggg gatcaatgta aaccgtacga ttagctttac
    11401 cttcgctttg ggttctgctc ttgcgggtgc ggctggtgtt ctgattgctc tttattataa
    11461 ctctcttgag cctttgatgg gggttactcc aggtcttaaa tctttcgttg ccgcagtact
    11521 tggtggtatc ggaattattc ctggtgcggc tcttggtggc tttgtgattg gtctattgga
    11581 aacctttgcg acagcctttg ggatgtcaga tttccgtgat gccattgttt atggaatctt
    11641 gttgttgatc ttgattgtcc gcccagctgg tatccttggt aagaatgtga aagagaaggt
    11701 gtaaacgatg aaggaaaatt taaaagttaa tattctatgg ttactccttt tgttagctgg
    11761 ctatagcttg attagtgtac tggtttcagt cggagtactt aatctattct atgtacagat
    11821 tttacaacaa attggaatta atattatttt ggctgttggt ctcaacttaa tcgttggttt
    11881 ttcaggacaa ttttcacttg gtcatgctgg tttcatggcg attggtgcct atgcagcagc
    11941 tattattggt tctaaatcac caacctacgg tgccttcttt ggagctatgc ttgtaggggc
    12001 tttgctttca ggagcagttg ccttacttgt cggcattcca accttgcgct tgaaggggga
    12061 ctatcttgcg gtagcaactc tgggtgtttc tgaaattatc cgtatcttta tcatcaatgg
    12121 tggaagtctt acaaatggtg cggcaggtat cttagggatt cctaacttta caacttggca
    12181 aatggtttac ttctttgtcg tgattacaac cattgcaacc ttgaacttct tgcgtagccc
    12241 aattggccgt tcaaccctct ctgttcgtga agatgaaatc gctgctgagt cagttggggt
    12301 taatacgact aaaattaaaa tcatcgcttt tgtctttggt gccattactg caagtattgc
    12361 tgggtcactt caggcaggat ttatcgggtc tgttgtaccg aaagattaca ccttcatcaa
    12421 ctcaatcaac gttttgatta ttgttgtatt tggtggactc ggttccatta caggtgcgat
    12481 tgtttcggct attgttctgg gaattttgaa tatgcttctc caagatgttg ctagtgtgcg
    12541 tatgattatt tacgctttgg ccttggtatt ggtaatgatt ttcagaccag gtggactcct
    12601 tggaacgtgg gaactgagcc tatcacgttt ctttaaaaaa tctaagaagg aggaacaaaa
    12661 ctaatggcat tacttgaagt aaaacagtta accaaacatt ttggtggtct aacagctgtt
    12721 ggagatgtga ctcttgaatt gaacgaaggg gaactggttg gattaatcgg tccaaacgga
    12781 gctgggaaaa ccaccctttt caaccttttg accggtgttt atgaaccaag cgagggaaca
    12841 gtaaccctag atggtcacct tttgaatggg aaatcacctt ataagattgc ctctttggga
    12901 cttggacgta ctttccaaaa tatccgtctt tttaaagatt taacagtttt agacaatgtt
    12961 ttgattgctt ttggaaacca tcacaaacag catgttttta ctagtttctt acgcttacca
    13021 gctttttaca agagtgaaaa agaattaaag gctaaagctt tggaattgtt gaaaatcttt
    13081 gatttagatg gtgatgcaga gactcttgct aaaaatcttt cctacggaca acaacgtcgt
    13141 ttggaaattg ttcgtgccct tgctacggaa cctaaaattc tcttcttaga tgaaccagca
    13201 gcaggtatga acccacagga aacagccgaa ttgactgagt taattcgtcg tatcaaagat
    13261 gaatttaaga ttacaatcat gttgattgaa cacgatatga atctggtcat ggaagtaaca
    13321 gaacgtatct acgtacttga atatggccgt ttaattgctc aaggaactcc agacgaaatt
    13381 aagaccaata aacgcgttat cgaagcttat ctaggaggtg aagcctaatg tctatgttaa
    13441 aagttgaaaa tctttctgtg cattacggta tgatccaagc agtccgtgat gtaagctttg
    13501 aagttaatga aggagaagtt gtttccctta tcggtgccaa cggtgcaggt aagacaacta
    13561 ttcttcgcac cttgtcaggt ttggttcgac caagttcagg aaagattgaa tttttaggtc
    13621 aagaaatcca aaaaatgcca gctcagaaaa tcgtggcagg tggtctttca caagttccag
    13681 aaggacgcca cgtctttcct ggcttgactg ttatggaaaa tcttgaaatg ggagctttct
    13741 taaagaaaaa tcgtgaagaa aatcaagcta acttgaagaa ggttttctca cgctttcctc
    13801 gtcttgaaga acggaagaac caagatgcag ctactctttc aggaggggaa caacaaatgc
    13861 ttgccatggg acgcgctctt atgtcaacac caaaacttct tcttttagat gaaccatcaa
    13921 tgggacttgc cccaatcttc atccaagaga tttttgatat cattcaagat attcagaagc
    13981 aaggaacaac cgtcctcttg attgaacaaa atgccaataa agcactcgca atctctgacc
    14041 gaggatatgt attggaaaca ggaaaaatcg tcctatcagg gacaggaaaa gaactcgctt
    14101 catcagaaga agtcagaaaa gcatatctag gtggctaaaa caatccagtg gattgtttta
    14161 gtcggcagat gaagattacg aagtaatcat caatatagtc cgggggacct ttttagccgg
    14221 tagattgaga ttgcaaacaa atctgcatct acattgaaag cttaatttct aataattgaa
    14281 aaaatcgaat gaaaagtttc ttaccttcat tcacagagct cgattttaga gctctttttg
    14341 ctagcttatt catacttttc taaattttga aaaagaaatg taagcgtttg atagatttgc
    14401 aaaaagattg tataataggg ataagaatag aaaaggagaa gtctcatggc agttaaagat
    14461 tttatgaccc gcaaggtagt ttatattagt ccagatacaa cagtatctca tgcagcagat
    14521 ttgatgagag agcaaggatt gcaccgcttg cctgttatcg aaaatgatca attggttggt
    14581 ttggtgactg agggaaccat tgcacaagca agtccatcta aagcaacaag tctttctatc
    14641 tatgagatga attatcttct gaataagaca aaagtaaaag atgtcatgat tcgcgatgtt
    14701 gtcactgtct caggctatgc tagtctagaa gatgcaactt atctgatgtt gaaaaataag
    14761 attggtattc tccctgtcgt agataaccat caagtatacg gagttattac tgaccgtgac
    14821 gttttccaag cctttcttga aattgcaggt tatggcgaag aagggattcg tgtacgcttt
    14881 gttacagaag atgaagttgg tgttcttgga aaaattattt ctttgattgt agaagaaaat
    14941 ttgaatatct cccatacagt caatattccg cgtaaggatg gtaaggtcat tatcgaagtg
    15001 caaatcgatg gatcaattga tttaccagcc ttgaaagaaa aatttgaagc aaatggtatt
    15061 caagtggaag aaatcgctcg cacttcagca aaagtcttgt aagaagggaa gcccaaaggc
    15121 ttcttttttc atgaaaaggg gattagagca aaagatggaa agaaatgata aaatatgcta
    15181 taatgaaatg atgtaaaaaa ggagtattta tggacatttc agtaattcgt cagaaaattg
    15241 acgcaaatcg tgaaaaatta gcttctttca gggggtctct ttgacctcga agggctagag
    15301 gaagagattg ccatcttgga aaacaagatg acagaacctg atttttggaa cgataatatt
    15361 gcggcccaaa aaacgtcgca agaattgaat gaattaaaaa acacttacaa taccttccat
    15421 aagatggaag agttgcagga tgaagtcgaa attttattgg attttttggc tgaagacgag
    15481 tcagtgcatg atgaactggt agcgcagtta gccgaacttg ataagataat gaccagctac
    15541 gagatgactc tactcttgtc agaaccttat gaccacaaca atgccatctt ggaaatccat
    15601 ccaggttctg gtggtactga ggcgcaggac tggggtgata tgttgcttcg tatgtatact
    15661 cgttatggta atgctaaagg ctttaaagtg gaagtgttgg attaccaagc aggtgatgag
    15721 gctggtatta agtcggtaac tttatcattt gaagggccta atgcctatgg tctcctcaag
    15781 tcagaaatgg gtgttcatcg cttagtgcga atctcaccat ttgactctgc caaacgtcgc
    15841 catacctctt tcacatctgt agaagtgatg ccagaattgg atgatactat tgaagtggaa
    15901 atccgtgaag atgatatcaa gatggatacc ttccgttcag gtggtgccgg tggacaaaac
    15961 gtcaataagg tttcaacagg tgtacgttta acccacattc caactggaat tgttgtccaa
    16021 tcaacagtgg atcgtaccca gtatggaaat agagatcgcg ccatgaagat gttgcaggct
    16081 aagctctatc aaatggagca agagaagaag gctgcggagg tagattctct caaaggtgag
    16141 aaaaaggaga tcacttgggg aagtcaaatc cgttcttatg tcttcacgcc ttatactatg
    16201 gtaaaagatc accgaactag ctatgaggtt gctcaggtag ataaggttat ggatggggac
    16261 ctagatggtt ttatcgatgc ttatctcaag tggcgaatta gctaagatag aaaggaactc
    16321 acatgtcaat tattgaaatg agagatgtcg ttaaaaaata cgacaacgga acgactgctc
    16381 tacgcggtgt ttcggttagc gttcaaccgg gggaatttgc ttacatcgta ggaccttcag
    16441 gagcagggaa gtcaactttt attcgttctc tgtatcgtga agtaaaaatc gataaaggaa
    16501 gtctatcagt tgctggtttt aatctggtta agatcaaaaa gaaagatgtc ccgcttctac
    16561 gtcgtagtgt tggggttgtc ttccaagatt ataaactgtt accaaagaaa actgtctatg
    16621 aaaatattgc ttacgctatg gaagtaatcg gggaaaatcg ccgtaatatc aaaagacgag
    16681 tgatggaagt tttggacttg gttggattga agcataaggt tcgttctttc ccaaatgaac
    16741 tctcaggtgg ggagcaacag cggattgcga ttgcgcgtgc aattgtaaat aatcccaaag
    16801 tattgatagc tgatgagcca acaggaaatc tggatccgga taattcatgg gaaattatga
    16861 atctcttgga acggattaac ctacaaggaa caactatttt gatggcgact cataatagcc
    16921 agattgtaaa taccttgcgc caccgtgtca ttgccattga aaatggccgt gtcgttcgtg
    16981 acgaatcaaa aggagagtat ggatacgatg attagtagat tttttcgcca tttatttgaa
    17041 gccttaaaaa gtttgaaacg aaatggttgg atgacagtag ctgctgtcag ttcagtcatg
    17101 attactttga ccttggtggc aatatttgca tctgttattt tcaatacagc gaaactagct
    17161 acagatattg aaaataatgt ccgtgtagta gtttatatcc gaaaggatgt ggaagataat
    17221 agtcagacaa ttgaaaaaga aggtcaaact gttacaaata atgactacca caaggtatat
    17281 gattctttga agaacatgtc tacggttaaa agtgttacct tttcaagtaa agaagaacaa
    17341 tatgaaaaat taaccgagat aatgggagat aactggaaaa tctttgaagg agatgccaat
    17401 cctctctatg atgcctatat tgtagaggca aacactccaa atgatgtaaa aactatagcc
    17461 gaagatgcta aaaaaattga aggtgtctct gaggttcaag atggcggtgc caatacagaa
    17521 agactcttca agttagcttc atttatccgt gtttggggac tagggattgc tgctttgtta
    17581 atttttatcg cagttttctt gatttcaaat accattcgta ttaccattat ttcccgcagt
    17641 cgcgaaattc aaatcatgcg cttggtcgga gctaaaaaca gttatatccg tggaccgttc
    17701 ttgttagaag gagcctttat cggtttattg ggagctatcg caccatctgt tttggtcttt
    17761 attgtttatc aaattgttta ccaatctgtc aacaaatcgt tggtagggca aaatctatcc
    17821 atgattagtc cagatttatt tagtccgttg atgattgccc tactatttgt gattggggtt
    17881 ttcattggtt cattgggatc aggaatatcc atgcgccgat tcttgaagat ttaggtaaaa
    17941 tagctgcttt tatgaggaga ttgtaaaatc tccttttttg ctacaagagt ttttgaaaag
    18001 agatgcgcag aagaaaagag cttccaaaga agtcccccag agaagacttc ttgatggcta
    18061 atttgaactt gaaatttggc gcactagaaa ttttttagaa aaaagtgtaa tttttttata
    18121 aaagccttta caaaaaaaat taaagtggta taattaaatc atagaaagtg caaacgtttt
    18181 cgtaaaacgt ttgcctaaat aaaaataaag gagatttgaa tgatgaaaga tacattcaaa
    18241 aatgtcttgt ctttcgaatt ttggcaaaaa ttcggtaagg ctttgatggt agttatcgcg
    18301 gttatgccgg ctgctggttt gatgatttca atcggtaagt ctatcgtgat gattaaccca
    18361 acctttgcac cacttgtcat cacaggtgga attcttgagc aaatcggttg gggggttatc
    18421 ggtaaccttc acattttgtt tgccctagcc attggaggaa gctgggctaa agaacgtgct
    18481 ggtggtgctt tcgccgctgg tcttgccttc atcttgatta accgtatcac tggtacaatc
    18541 tttggtgtat caggcgatat gttgaaaaat ccagatgcta tggtaactac tttctttggt
    18601 ggttcaatca aagttgctga ttactttatc agtgttcttg aagctccagc cttgaacatg
    18661 ggggtattcg tagggattat ctcaggtttt gtaggggcaa ctgcttacaa caaatactac
    18721 aacttccgta aacttcctga tgcactttca ttcttcaacg ggaaacgttt cgtaccattt
    18781 gtagttattc ttcgttcagc aatcgctgca attctacttg ctgctttctg gccagtagtt
    18841 caaacaggta tcaataattt cggtatctgg attgccaact cacaagaaac tgctccaatt
    18901 cttgcaccat tcttgtatgg tactttggaa cgtttgctct tgccatttgg tcttcaccac
    18961 atgttgacta tcccaatgaa ctacacagct cttggtggta cttatgacat tttaactggt
    19021 gcagctaaag gtactcaagt attcggtcaa gacccactat ggcttgcatg ggtaacagac
    19081 cttgtaaacc ttaaaggtac tgatgctagt caataccaac acttgttaga tacagtacat
    19141 ccagctcgtt tcaaagttgg acaaatgatc ggttcattcg gtatcttgat gggtgtgatt
    19201 gttgctatct accgtaatgt tgatgctgac aagaaacata aatacaaagg tatgatgatt
    19261 gcaacagctc ttgcaacatt cttgacaggg gttactgaac caatcgaata catgttcatg
    19321 ttcatcgcaa cacctatgta tcttgtttac tcacttgttc aaggtgctgc cttcgctatg
    19381 gctgacgtcg taaacctacg tatgcactca ttcggttcaa tcgagttctt gactcgtaca
    19441 cctattgcaa tcagtgctgg tattggtatg gatatcgtta acttcgtttg ggtaactgtt
    19501 ctctttgctg taatcatgta ctttatcgca aacttcatga ttcaaaaatt caactacgca
    19561 actccagggc gcaacggaaa ctacgaaact gctgaaggtt cagaagaaac cagcagcgaa
    19621 gtgaaagttg cagcaggctc tcaagctgta aacattatca accttcttgg tggacgtgta
    19681 aacatcgttg atgttgatgc atgtatgact cgtcttcgtg taactgttaa agatgcagat
    19741 aaagtaggaa atgcagagca atggaaagca gaaggagcta tgggtcttgt gatgaaagga
    19801 caaggggttc aagctatcta cggtccaaaa gctgacattt tgaaatctga tatccaagat
    19861 atccttgatt caggtgaaat cattcctgaa actcttccaa gccaaatgac tgaagtacaa
    19921 caaaacactg ttcacttcaa agatcttact gaggaagttt actcagtagc agacggtcaa
    19981 gttgttgctt tggaacaagt aaaggatcca gtatttgctc aaaaaatgat gggtgatgga
    20041 tttgcagtag aacctgcaaa tggaaacatt gtatctccag tttcaggtac tgtatcaagc
    20101 atcttcccaa caaaacatgc ttttggtatt gtgacggaag caggtcttga agtattggtt
    20161 cacattggtt tggacacagt aagtcttgaa ggtaaaccat ttacagttca tgttgctgaa
    20221 ggacaaaaag ttgcagcagg agatctcctt gtcacagctg acttgaatgc tatccgtgca
    20281 gcaggacgtg aaacttcaac agtagttgtc ttcacaaatg gtgatgcaat taaatcagtt
    20341 aaattagaaa aaacaggttc tcttgcagct aaaacagcag ttgctaaagt agaattgtaa
    20401 tatacttgag gttggaagct gtattccaac ctcttatttt gggagaaaag aatgaaattt
    20461 ttaacactca atactcatag ttggatggag aaagaagcag aggaaaaatt ccagattttg
    20521 cttgaagata ttcttgaaaa ggactatgat ttgatttgtt tccaagaaat taatcaggag
    20581 atgacctcgt cagaggtgga ggttaatgac ctttatcaag ctttgccagc agctgagcct
    20641 attcaccaag atcactatgt tagactcttg gttgaaaagt tatctgagca agggaaaaat
    20701 tactactgga cctgggccta taaccatatc ggctatgacc gctaccacga aggtgtggct
    20761 atcttgtcta aaacacctat tgaagccaga gaaattttgg tttcagatgt ggatgatcca
    20821 acagactatc atactcgccg tgttgcccta gctgaaactg tagtcgatgg caaggagcta
    20881 gcagttgcca gtgttcatct ctcttggtgg gataaaggtt ttcaagaaga atgggcacga
    20941 tttgaggctg tcttgaaaaa attgaacaag ccacttttac tagctggaga ttttaacaac
    21001 ccagcaggtc aggaaggtta ccaagctatt ttagctagtc cattaggctt acaagacgca
    21061 tttgaagttg ctcaagagaa aagtggtagc tatactgttc cgcctgaaat tgatggctgg
    21121 aaagggaaca ctgaacccct tcgaatcgat tatgtcttta ctaccaaaga gttagcggtg
    21181 gaaaatttac atgtcgtatt tgatggtaac aagagtccac aagtgagtga tcactatggc
    21241 ttgaatgcta tgttaaactg gaaataataa ctgaaaagag gttggaacta ta
//
LOCUS       DAVLXF010000026        20896 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLXF010000026 DAVLXF010000000
VERSION     DAVLXF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.026"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            408..646
                     /locus_tag="VYO12_000929"
                     /pseudo
     CDS             408..646
                     /locus_tag="VYO12_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            790..2019
                     /locus_tag="VYO12_000930"
     CDS             790..2019
                     /locus_tag="VYO12_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1587568.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2039..4735
                     /gene="ppc"
                     /locus_tag="VYO12_000931"
     CDS             2039..4735
                     /gene="ppc"
                     /locus_tag="VYO12_000931"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1587569.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    4818..5035
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5148..6152
                     /locus_tag="VYO12_000932"
     CDS             5148..6152
                     /locus_tag="VYO12_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1587570.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6221..7087
                     /locus_tag="VYO12_000933"
     CDS             6221..7087
                     /locus_tag="VYO12_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587571.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7084..7842
                     /locus_tag="VYO12_000934"
     CDS             7084..7842
                     /locus_tag="VYO12_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587572.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            7932..9692
                     /gene="dnaG"
                     /locus_tag="VYO12_000935"
     CDS             7932..9692
                     /gene="dnaG"
                     /locus_tag="VYO12_000935"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1587573.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            9695..10804
                     /gene="rpoD"
                     /locus_tag="VYO12_000936"
     CDS             9695..10804
                     /gene="rpoD"
                     /locus_tag="VYO12_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1587574.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            10818..11147
                     /locus_tag="VYO12_000937"
     CDS             10818..11147
                     /locus_tag="VYO12_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1587575.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11262..12305
                     /gene="cpoA"
                     /locus_tag="VYO12_000938"
     CDS             11262..12305
                     /gene="cpoA"
                     /locus_tag="VYO12_000938"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1587576.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12321..13646
                     /locus_tag="VYO12_000939"
     CDS             12321..13646
                     /locus_tag="VYO12_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1587577.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            13677..13853
                     /locus_tag="VYO12_000940"
     CDS             13677..13853
                     /locus_tag="VYO12_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1587578.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            13912..14046
                     /locus_tag="VYO12_000941"
     CDS             13912..14046
                     /locus_tag="VYO12_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1587579.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14110..15420
                     /gene="obgE"
                     /locus_tag="VYO12_000942"
     CDS             14110..15420
                     /gene="obgE"
                     /locus_tag="VYO12_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1587580.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15430..15588
                     /locus_tag="VYO12_000943"
     CDS             15430..15588
                     /locus_tag="VYO12_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587581.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            16638..17897
                     /locus_tag="VYO12_000944"
     CDS             16638..17897
                     /locus_tag="VYO12_000944"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1587582.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            17913..18464
                     /locus_tag="VYO12_000945"
     CDS             17913..18464
                     /locus_tag="VYO12_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1587583.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            18457..19734
                     /locus_tag="VYO12_000946"
     CDS             18457..19734
                     /locus_tag="VYO12_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1587584.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            19750..20610
                     /locus_tag="VYO12_000947"
     CDS             19750..20610
                     /locus_tag="VYO12_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1587585.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         6380 a         3602 c         4708 g         6206 t
ORIGIN      
        1 aataactttt ccgagagata ttaaaaccat caataaaata gagcgatact ttatatgcca
       61 tgatacaaat gatatacaat gatacttctg accgttcagg ctgccatcgt aaaagagcag
      121 caagtgaaat tcttatgatg acttcatcag tcatgccacg ttaaatgtgt gagtttgtta
      181 gagaaacgca attaatcctc aaaaggttcc ccgaaccttt tgagttccac agacgcatca
      241 cgtggagtgt gtaagcttgt tgctaaaagc gtaaaaacct tggaacgaaa ggaataatag
      301 acttcctgcg aaacaaaaat acaatacaat aaaactatgg atgatgaagc aagtaaacaa
      361 ttaagcgata gccgtttcaa gagccttgta ggtgttcagc gcacgagatg ttagctgtgt
      421 taaaaacagc ttatcaacgc aacgtaaacg cgcaaaaggt ggacgaaaaa ccaaattaag
      481 cctagacgat ctcctcatgg taactattca atacatgcga gaatagcaca cttatgaaca
      541 aattgcggct gattttggca ttcacgaaag caacataatt agtcggagtc aatggattga
      601 agcaactctt attcaaaatg gttttacgat ttcaaattct gccttaattc tgtaaaaaca
      661 gtaaaattcg aaggattgta aggtaagagt ttttttcttt ctgaaaaaat ggtataatag
      721 caatcaaaac tagaaaataa aacggaattt ggaacagatt tgtctgtatc ctagtagagt
      781 ggtgatacta tgaagattag taagaggcac ttattaaatt attccatctt gattccctac
      841 ttgcttttat ctattttggg cttgattgtg gtctattcga ccaccagtgc tattttaatt
      901 gaagaaggca agagcgcctt gcagttggtt cgaaaccaag gaatcttttg gattgttagt
      961 ttgatactga ttgccttaat ttataaattg agactagatt ttttgagaaa tgagcgacta
     1021 atcattttag ttatattaat agaaatgctt ttattgttct tggctcgttt tattggtatt
     1081 tccgtaaacg gggcatacgg ttggatttcg gttgcaggaa taactattca gccagctgag
     1141 tacttaaaaa tcattattat ttggtattta gctcaccgat tctccaaaca gcaagaagaa
     1201 atagctactt atgattttca agttttgact caaaatcaat ggcttccccg tgcttttaat
     1261 gattggcgat tcgttctcct agttctgatt ggaagtttgg gaattttccc tgatttagga
     1321 aatgcgacta ttttagtctt ggtttccttg attatgtata cagttagtgg aatcgcttat
     1381 cgctggtttt caaccattct ggcgctcgta tctgccgctt ctgtctttgt cttgaccact
     1441 atcagcctaa tcggtgttga gaccttttca aaaattccag tatttggcta tgtagccaag
     1501 cgctttagtg ccttttttaa tccttttgcc gatcgtgctg atgcaggtca ccagttagct
     1561 aattcttatt ttgccatggt caatggcggt tggtttggtc taggtcttgg aaactcgatt
     1621 gaaaaacgag gttatttgcc agaagctcat acagactttg tcttttctat cgtgattgaa
     1681 gaatttggct ttgttggtgc cagtcttatt ttagctctct tatttttcat gattttgcgg
     1741 attatcttgg tcggtatccg agcggagaat cctttcaatg ccatggttgc actcggtgtc
     1801 ggagggatga tgttggttca ggtatttgtc aatatcggag ggatttcggg cttgattcca
     1861 tctacaggag taaccttccc cttcttatcc cagggtggaa atagtcttct agtcttatca
     1921 gtggcagtag cctttgtctt aaatattgat gccagtgaaa aacgcgctaa attgtaccga
     1981 gaattggaaa atcaaccaat gaaccttctg ttgaagtagg ataaagaaag gatagtttat
     2041 gtctcttcaa aaattagaaa attatagtaa taaaagtgtt gtgcaagaag aagtcttgat
     2101 tctaacagaa ttactggaag atattactaa aaatatgctt gccccagaga cctttgaaaa
     2161 aataatacag ttgaaagaat tatcaacgca ggaagattat caaggtctaa accgtctagt
     2221 gactagctta tcaaatgatg aaatggtcta tatttcacgc tatttctcta tcttgcctct
     2281 tttgattaat atttcagagg atgtggattt agcttatgaa atcaatcatc aaaataatat
     2341 tgatcaggac tatttaggta aattatctac aacgattaaa ttggtagcag aaaaggaaaa
     2401 tgccgttgag atcctagaac acttgaatgt tgtccctgtt ttgacagccc atccaacaca
     2461 agtgcaacgc aaaagtatgt tggatttaac aaatcatatt catagtcttt tgcgtaaata
     2521 ccgtgatgtt aagttggggt tgattaataa agataaatgg tacaatgatt tgcgtcgtta
     2581 catcgaaatt atcatgcaga cagacatgat tcgtgagaaa aaattaaaag tgactaacga
     2641 aatcacgaat gctatggaat attataacag ctcctttttg aaagctgtac ctcatttgac
     2701 gacggagtat aagcgcttag cgcaagcgca tggtctgaat ttaaaacagg ctaaaccaat
     2761 caccatgggt atgtggatag gtggtgaccg tgatggaaat ccatttgtta cagcaaagac
     2821 cttgaagcag tctgcactca ctcagtgtga agtcatcatg aactactatg ataaaaagat
     2881 ttaccaactt tatcgtgaat tttctctttc aactagcatt gtcaacgtca gcaagcaagt
     2941 cagagaaatg gctcgtcaat ccaaggataa ctcgatttac cgcgaaaaag agctttaccg
     3001 tcgtgccttg tttgatattc aatcaaaaat tcaggcaact aaaacctatc tgattgagga
     3061 tgaagaagtt gggactcgtt atgaaaccgc caatgatttc tacaaggatt tgattgccat
     3121 tcgagattct ctactagaaa ataagggtga gtccttgatt tcaggtgatt ttgtggaatt
     3181 attgcaggca gtagagatat ttggttttta cttagcgtca attgatatgc gacaagactc
     3241 tagcgtctat gaagcctgtg tggcagaact cttgaaatca gcaggaattc attctcgtta
     3301 tagcgagttg agcgaagaag aaaagtgtga ccttctcttg aaagaattag aagaagatcc
     3361 ccgaattctt tctgcgactc acgcagaaaa atcagaatta ttagcaaaag aattagctat
     3421 ttttaagacg gctcgtgttt tgaaagataa gttgggagat gatgtcatcc gtcagaccat
     3481 catttcacat gcaaccagcc tttctgatat gctagaatta gctattctgt taaaagaagt
     3541 aggactggtg gatacggaaa gggcgcgtgt tcagattgtt cccctttttg aaacaattga
     3601 agacttggat cattcagagg aaacaatgag aaaatatctt tctcttagcc ttgccaaaaa
     3661 atggattgac tcacgaaata actaccaaga aatcatgctt ggctactctg acagtaataa
     3721 agatggcggt tacctgtcat catgttggac cctctacaag gctcaacaac aattgactgc
     3781 tattggagat gaatttggcg ttaaggttac cttcttccat ggtcgtggtg gtactgtcgg
     3841 tcgtggtggt gggccaacct atgaagccat tacatctcaa ccgctcaagt ctatcaagga
     3901 tcgtatccgc ttgacggagc agggtgaagt aattgggaat aaatacggta acaaagacgc
     3961 cgcttactat aaccttgaaa tgctagtatc ggcagctatt aaccgtatga ttactcagaa
     4021 gaagagcgat accaataccc caaatcgtta tgaagccatt atggatcaag tagtggaccg
     4081 tagttacgat atctaccgtg atttggtctt tggtaatgag catttctatg attatttctt
     4141 cgagtcaagt ccaatcaagg ctatttcaag ttttaatatt ggttctcgtc cagccgctcg
     4201 taagactatt actgaaatcg gtggtttgcg tgccatccct tgggtattct catggtcaca
     4261 gagtcgtgtt atgttccctg gatggtacgg ggttggttca agcttcaagg aatttatcaa
     4321 taaaaatcca gagaatattg ctatcttacg agatatgtac caaaattggc ctttcttcca
     4381 atcgcttctt tcaaatgttg atatggtttt gtcaaaatca aatatgaata ttgcttttga
     4441 atatgctaaa ctttgtgaag acgagcaagt taaggccatc tatgagacta ttttaaatga
     4501 atggcaagtt actaagaacg ttatcttggc tattgaagga catgacgaac tcttagctga
     4561 caatccatat ctaaaagcta gtctggatta ccgtatgcct tactttaata ttctcaacta
     4621 tattcagtta gagttgatta aacgccaacg tcgtggagaa ttgtccagtg atcaagaacg
     4681 attgattcat atcaccatca acggaattgc gacaggattg cgtaattcag gttgataatt
     4741 ttcaagagtg aatgctaaaa gtgaatatca aaaaaattct aatagactat tgacaagtag
     4801 tttaaaaatg atataattta accattcaga aaagtaatca tacaaacttt ttagagagtc
     4861 tgtggtagct gaaaacagat aagtggcgat gatgaaaatt gggctgaatg ctatttagaa
     4921 tttgaaatta taaaaattcg gtaagcacac cttacagtgc atctcgttat tgcgagactg
     4981 agcgataggg aaattcccta taattgaggt ggtaccgcgc atcgacgtcc tcacacaagt
     5041 tttttgtgtg aggatttttt tgatggaggt tagtatggaa agaaaacgat ggcgtcgctt
     5101 gtttagataa gtgaaatatg ttaaaggaaa taaaaaggag aaacagaatg aaaaataaac
     5161 gtttaattgg aattattgct gcattagcag tcttagtagc aggaagcttg atttattctt
     5221 caatgaataa atcagaagct cagaataata aggatgagaa gaaaataacc aagattggtg
     5281 tgcttcaatt tgtgagccat ccatcccttg atttgattta taaagggatc caagatggac
     5341 ttgcagaaga aggatataaa gatgatcaag ttaaaattga ttttatgaac tcagaaggtg
     5401 atcaaagtaa ggttgcgaca atgagtaaac aattggttgc aaatgggaat gaccttgtgg
     5461 ttggtattgc aacaccagca gcccaagggt tggctagtgc aacaaaagac ctaccagtta
     5521 tcatggccgc tattacagac ccaattggtg ctaacttggt taaagatttg aaaaaaccag
     5581 gtggtaacgt tacaggggta tctgaccaca atccagctca acaacaaatt gaactcatca
     5641 aggctctgac accgaatgtg aaaacaatcg gagctcttta ctcaagtagc gaagacaatt
     5701 caaaaacaca ggttgaagaa tttaaggctt atgctgaaaa agcaggtctg acagtggaaa
     5761 catttgcagt tccttcaaca aatgaaattg cctcaactgt cactgttatg actatcaagg
     5821 tagatgctat ttgggttcca attgataaca ccattgcatc aggatttcca acggttgtct
     5881 ctagcaatca aagttctaag aaaccaattt atcccagtgc gacagctatg gtagaagtag
     5941 gtggtttggc atcagttgta attgaccaac atgaccttgg tgtggcaaca ggtaaaatga
     6001 ttgtgcaagt cttgaaaggt gcaaaaccag ccgatacccc agtcaatgtc ttttcaactg
     6061 gtaagtcagt catcaataaa aaaatagcac aagaactagg tattactatt cctgagtctg
     6121 ttctcaaaga agcaggacaa gtcatcgaat aaataaggta gggaggagga gactcttccc
     6181 attttttaaa aagatgaata tagaaaggat taaataaaat atgattgttt ccattatttc
     6241 tcaaggattt gtctgggcta ttctaggtct gggaatcttt atgacattta ggattttaaa
     6301 ctttccagat atgacgacag aaggttcctt ccctcttggg ggagctgttg ctgtcacttt
     6361 gataaccaaa ggcgtgaacc catttttagc gacacttgtt gctgtaggag caggttgttt
     6421 ggctggaatg gcagcaggcc ttctttatac aaaagggaag atcccaacct tgctctcagg
     6481 gattttggtg atgacttctt gtcactcaat catgctcttg attatgggac gtgcgaattt
     6541 aggcctgctt ggaaccaagc aaattcagga tgttttgcct tttgattcgg atttgaatca
     6601 actcttgaca ggtctcatct ttgtgagcat tgttattgct ctcatgctct ttttcttgga
     6661 cactaaactc ggacaagcct atattgctac aggggataat cctgatatgg ctagaagttt
     6721 cgggattcat actggacgca tggagctcat gggcttggtc ttatcaaatg gtgtgattgc
     6781 ccttgcaggt gccctcattg ctcagcaaga aggctatgcc gatgtgtctc gagggatcgg
     6841 ggttatcgtt gtggggcttg caagtttgat tattggagaa gttattttca agagtttgag
     6901 cttggcagag cgtttggtta ctatcgttgt aggttctatc gcttatcaat ttttagtgtg
     6961 ggcagttatc gcacttggct ttaatacaag ttaccttcgt ttatacagtg ccttgatttt
     7021 agcagtctgc ctcatgattc caacatttaa gcaaacaatc ttgaaaggag ccaagttaag
     7081 caaatgacag caattgtaga attaaaaaat gcaaccaaaa ttgttaaaaa tggctttgat
     7141 gaagaaaaga ttattttaaa tgatgtttcc ttagaaattt ttgaacggga ctttatcacg
     7201 attttgggcg gaaatggtgc tggaaaatca accctcttta acactatagc agggacctta
     7261 tcactaacta gtggaactat ccgtatttta ggtgaagatc tcactaagtt ttcacccgag
     7321 aagcgtgcca agtacctgtc tcgtgtcttc caagatccaa agatggggac agctccccgt
     7381 atgacggtcg ctgaaaatct tttaatcgcc aagtttcgtg gtgagaagcg tggattgtta
     7441 ccacgacgct tgactagcta taaggatgaa tttcaggcaa ccattgaaaa agtaggaaat
     7501 ggtcttgaga aacacttgaa tacaccgatt gagttcttat caggtggaca aagacaggct
     7561 ttgagtctct tgatggcaac cttgaagcga cctgaattac tcctgttaga tgagcatact
     7621 gctgccctgg atccaaagac tagtgttgct ttgatggaat tgacagatga atttgttaag
     7681 aaagatcagc taacagccct tatgattact catcatatgg aagatgctct caaatacggc
     7741 aatcgcttga ttgtcatgaa agaaggacgg attatccaag atttaaacca agaagaaaaa
     7801 gcaaaaatga aaatctctga ttattatcaa ctctttgaat aaagtaggga aaatgagtga
     7861 aatggtttac tttttttctg aaataaagta tactatataa agtaaactat gataacatgg
     7921 aggtattgtg tatggttgac aaacaagtca ttgaagaaat caaaaacaat gccaacattg
     7981 tggaagtcat aggagatgtg atttctttac aaaaggcagg acggaactat ctagggctct
     8041 gtccttttca tggtgaaaaa acaccttctt tcaacgttgt agaggacaag cagttttacc
     8101 actgttttgg ttgtggtcgc tcaggtgatg tctttaaatt catcgaggag taccaagggg
     8161 ttccctttat agaggctgtc caaatcttag gtcagcgtgt cgggattgag gttgaaaaac
     8221 cgctttatag tgaacagaag ccagcctcgc ctcaccaagc tctttatgat atgcacgaag
     8281 atgcggctaa attttaccat gctattctca tgacaacgac tatgggcgaa gaggccagaa
     8341 attaccttta tcagcggggt ttgacagatg aagtgcttaa acatttttgg attggtttag
     8401 cacctccaga acgaaactat ctctatcaac gtttgtctga tcagtatcgt gaagaggatt
     8461 tactggattc aggcctgttt tatctctcgg atgccaatca atttgtagac acctttcaca
     8521 atcgcattat gtttcccctg acaaatgacc aaggaaaggt cattgccttc tcaggtcgta
     8581 tctggcaaaa aacggattca caaacttcta agtataaaaa cagccgatcg actgcaattt
     8641 ttaacaaaag ttacgaatta tatcatatgg atagggcaaa aaaatcttct ggaaaaacta
     8701 gtgagattta cctgatggaa ggattcatgg atgttattgc agcctatcgg gctggaatcg
     8761 aaaatgctgt ggcgtcgatg ggaacggcct tgagtcgaga gcatgttgag catctgaaaa
     8821 ggttaaccaa gaaattggtt cttgtttacg atggagataa ggctgggcaa gccgcgatat
     8881 tgaaagcatt ggatgaaatt ggtgatatgc ctgtgcaaat cgttagcatg cctgataact
     8941 tggatcctga tgagtatcta caaaaaaatg gtccagaaga cttggcctat ctattaacaa
     9001 aaactcgtat tagtccgatt gagttctaca ttcatcagta caaacctgaa aacagtgaaa
     9061 atctgcaggc tcagattgag tttattgaaa aaatagctcc cttgattgtt caagaaaagt
     9121 ccatcgctgc tcaaaacagc tatattcata ttttagctga cagtctggcg tcctttgatt
     9181 atacccagat tgagcagatt gttaatgaga gtcgtcaggt gcaaaggcag aatcgcatgg
     9241 aaagaatttc cagaccgacg ccaatcacca tgcctgtcac caagcagtta tcggctatta
     9301 tgagggcaga agcccatcta ctctatcgga tgatggaatc ccctcttgtt ttgaacgatt
     9361 accgtttgcg agaagacttt gcatttgcta cacctgaatt tcaggtctta tatgacttgc
     9421 ttggccagta tggaaatctt cctccagaag ttttagcaga gcagacagag gaagttgaaa
     9481 gagcttggta ccaagtttta gctcaggatt tgcctgctga gatatcgccg caggaactta
     9541 gtgaagtaga gatgactcga aacaaggctc tcttgaatca ggacaatatg agaatcaaaa
     9601 agaaggtgca ggaagctagc catgtaggag atacagatac agccctagaa gaattggaac
     9661 gtttaatttc ccaaaagaga agaatggagt aataatggca acaaaacaaa aagaagtaac
     9721 aacatttgac gtacaggtag cagaatttat ccgtaatcat aagcaaaaag ggacagcaac
     9781 agatgatgaa atcaatgcaa gtctggttat tccttttacc ttggacgctg atgggattga
     9841 agatctcttg caacggattc aggatgcagg gatttctatc acagataacg aaggaaatcc
     9901 aagtgcgcgt gttcttagca atgaagaaga accagaactc agcgatgagg acttgattgg
     9961 atcaacttct gctaaggtca atgaccctgt ccgtatgtac ttgaaagaaa taggggtcgt
    10021 tcctctcttg accaatgaag aggagaaaga gttggcactg gctgttgaag ctggcgatat
    10081 cgaagccaaa caacgtcttg cggaagccaa tcttcgtttg gttgtttcca ttgccaaacg
    10141 ctatgtcggt cgtggcatgc agttccttga cttgattcaa gaaggaaata tgggcttgat
    10201 gaaggcggtt gacaagtttg actattctaa agggttcaag ttttcaactt atgcaacttg
    10261 gtggattcgt caggctatca ctcgtgctat tgcggaccaa gctcgtacca tccgtatccc
    10321 agttcacatg gttgaaacta tcaataaatt ggttcgtgaa cagcggaatc tccttcaaga
    10381 attggggcaa gatccgacac cagaacagat tgctgaacga atggatatga cacctgataa
    10441 ggttcgtgaa atcttgaaga ttgcccaaga accagtatct cttgaaactc ctatcggtga
    10501 agaggacgat agccaccttg gagactttat cgaagatgaa gtgattgaaa atccagtgga
    10561 ttatacgact cgtatcgtct tgcgtgagca attggatgaa atcttagata ctcttacaga
    10621 ccgtgaagaa aatgttctgc gtctacgttt tggactagat gatggaaaaa tgcgcacact
    10681 tgaagatgtg gggaaagtct ttaacgtaac tcgtgagcgt atccgtcaga ttgaagcaaa
    10741 ggctttgaga aaactacgcc aaccaagtcg cagcaaaccg cttcgtgatt ttattgaaga
    10801 ctaagagtga ggaaaatatg gcttatacag aagagcaaat tgaaaacatc aaaacacgga
    10861 ttttaacagc cttggaagaa gtcatcgacc ctgagttggg aatcgatatt gtcaatcttg
    10921 gtttgatcta tgagattcgt tttgacggtg acacagggca aacagagatt gacatgactt
    10981 tgacaactat gggttgtccc ttagcagacc ttttgacaga ccagatttat gatgctatga
    11041 tagaggttcc agaagtaacg gatactgagg ttaaattagt ctggtatcca gcctggacag
    11101 ttgaaaaaat gagtcgctat gcccgcattg ccctaggcat taagtaaaca tataaaagca
    11161 tgtgagagac tagaggaaaa gcgaggaaat ttcccctctt ttcctctagt ctctcctttc
    11221 ttttgctgat tttattcaaa gaaaatgata taatagtagt tatggagaaa aagaaattac
    11281 gcatcaatat gttgagttca agtgagaaag tagcaggaca gggagtttca ggtgcttacc
    11341 gtgaattagt tcgtcttctt caccgtgctg ccaaggacca attgattgtt acagaaaatc
    11401 ttccaatcga ggcagatgtg actcactttc atacgattga ttttccctat tatttatcaa
    11461 ccttccaaaa gaaacgctca gggagaaaga ttggctatgt gcatttcttg ccagctacac
    11521 ttgaaggaag tttgaaaatt ccatttttct taaagggaat tgtgaaacgc tatgtatttt
    11581 ctttttacaa ccggatggag cacttggttg tggtcaatcc tatgtttatt gaggatttgg
    11641 tagcagctgg tattccacgt gaaaaagtga cctatattcc taactttgtc aacaaggaaa
    11701 aatggcatcc tctaccacaa gaagaggtag tcagactgcg cacagatctt ggtcttagtg
    11761 acaatcagtt tatcgtagta ggtgctgggc aagttcagaa acgtaaaggg attgatgact
    11821 ttatccgtct ggctgaggaa ttgcctcaga ttacctttat ctgggctggt ggcttctctt
    11881 ttggtggtat gacagatggt tatgaacact ataagaaaat tatggaaaat ccccctaaaa
    11941 atttgatttt tccaggcatt gtatcgccag agcggatgcg cgaattgtat gctctagcgg
    12001 atcttttctt gttgcctagt tacaatgagc tctttcctat gactatttta gaagctgcga
    12061 gttgtgaggc tcctattatg ttgcgtgatt tagatctcta taaggtgatt ttggagggaa
    12121 attatcgggc gacagcgggt agagaagaga tgaaagaggc tattttggaa tatcaagcaa
    12181 atcctgctgt cttaaaagat ctcaaagaaa aggctaagaa tatttccaga gagtattctg
    12241 aagagcatct gttacaaatc tggttggact tttatgagaa acaagccgct ttagggagaa
    12301 agtaaaaagt gaggtaatct atgcgaattg gtttatttac agatacctat tttcctcagg
    12361 tttctggtgt tgcgaccagt attcgaacct tgaaaacaga acttgaaaag cagggacatg
    12421 ctgtttttat ctttacgacg acagataagg atgtcaatcg ctacgaagat tggcaaatta
    12481 tccgcattcc aagtgttcct ttctttgctt ttaaggatcg tcgctttgcc taccgaggtt
    12541 ttagcaaggc acttgaaatt gctaaacagt atcagctaga tattatccat actcagacag
    12601 aattttctct tggcctgttg gggatttgga ttgcgcgtga attgaaaatt ccagtcatcc
    12661 atacctatca cacccagtat gaagactatg tccattatat tgctaagggg atattgatcc
    12721 ggccgagtat ggtcaagtat ctggttagag gtttcctgca tgatgtggat ggggttattt
    12781 gccctagtga gattgtccgt gacttgctat ctgattataa ggtcaaggtt gaaaaacggg
    12841 tcattcctac tgggattgaa ttagccaagt ttgagcgtcc ggaaatcaag caggaaaatt
    12901 tgaaagaact gcgtagtaaa ctagggattc aagatggtga aaagacgttg cttagtcttt
    12961 cgagaatctc ctatgaaaaa aatattcaag cagttttagc agcctttgct gatgttctga
    13021 aagaggaaga caaggttaaa ctggtagtag ctggggatgg cccttatctg aatgacctca
    13081 aagagcaagc ccagaaccta gagattcaag actcagtcat ctttacaggg atgattgctc
    13141 ctagtgagac ggctctttac tataaagcgg cggatttctt catttcggca tcgacaagcg
    13201 aaacgcaagg tttgacctac ttggaaagct tagccagtgg aacacctgtc attgctcacg
    13261 gaaatcctta tttgaacaac ctcatcagtg ataaaatgtt tggagccttg tactatggag
    13321 aacatgattt ggctggtgct attttggaag ccctgattgc aacaccagac atgaatgagc
    13381 ataccttatc agagaaattg tatgagattt cagctgagaa ctttgggaaa cgagtgcatg
    13441 agttttatct ggatgccatt atttcaaata acttccagaa agatttggct aaagatgata
    13501 cggtcagtca gcgtatcttt aggacagttt tgtatcttcc gcaacaggtg gttgctgtac
    13561 ctgtaaaagg atctagacgc atgttgaagg cttcaaaaac acagttgatc agtatgagag
    13621 actattggaa agaccatgaa gaatagaaag aggaacagct atgaaaaaaa caattaatga
    13681 gaagcggtcg tgataaaaag attgcgggtg tttgtgctgg ggtggcccat tatctggata
    13741 tggatccgac tatcgttcaa gtcatttggg gtgttcttac ttgctgttac ggagctggaa
    13801 ttgtagctta cattatttta tggattatcg caccagtagc aactgactat tgaaaactag
    13861 ctcaattcat gctaagataa tagaaaatag aatgtaacga aggagagaaa aatggcattt
    13921 ggagataatg gaaatcgtaa aaaaactatg tttgagaaaa taaccttgtt tatcgtgatt
    13981 atcatgctag tagcaagttt attgggaatt tttgcaactg caattggtgc cctcagtaat
    14041 ctataaaata gattcaagaa aatttagtga ctgggatttc ccagcccttt tttaaagtga
    14101 gaagaaataa tgagtatgtt tttagataca gctaagatta aggtcaaggc tggtaatggt
    14161 ggcgatggta tggttgcctt tcgtcgtgaa aaatatgtcc ctaatggagg cccttggggt
    14221 ggtgatggtg gtcgtggagg caatgtggtc ttcgttgtag acgaaggact acgtaccttg
    14281 atggatttcc gctacaatcg tcatttcaag gctgattctg gtgaaaaagg gatgaccaaa
    14341 gggatgcatg gtcgtggtgc tgaggacctt agagttcgag taccacaagg tacgactgtt
    14401 cgtgatgcgg agactggcaa ggttttaaca gatttgattg aacatgggca agaatttatc
    14461 gttgcccacg gtggtcgtgg tggacgtgga aatattcgtt tcgcgacacc aaaaaatcct
    14521 gcaccggaaa tctctgaaaa tggagaacca ggtcaggaac gtgagttaca attggaacta
    14581 aaaatcttgg cagatgtcgg tttagtagga ttcccatctg tagggaagtc aacactttta
    14641 agtgttatta cctcagctaa gcctaaaatt ggtgcctacc actttaccac tattgtacca
    14701 aatttaggta tggttcgcac ccaatcaggt gaatcctttg cagtagccga cttgccaggt
    14761 ttgattgaag gggctagtca aggtgttggt ttgggaactc agtttctccg tcacatcgag
    14821 cgtacacgtg ttatccttca catcattgat atgtcagcta gcgaaggccg tgatccatat
    14881 gaggattacc tagctatcaa taaagagctg gagtcttaca atcttcgcct catggagcgt
    14941 ccacagatta ttgtagctaa taagatggac atgcctgaga gtcaggaaaa tcttgaagaa
    15001 tttaagaaaa aattgtctga aaattatgat gaatttgaag agttaccagc tatcttccca
    15061 atttctggat tgaccaagca aggtctggca acacttttag atgctacagc tgaattgtta
    15121 gacaagacac cagaattttt gctctacgac gagtccgata tggaagaaga agcttactat
    15181 ggatttgacg aagaagaaaa agcctttgaa attagtcgtg atgacgatgc gacatgggta
    15241 ctttctggtg aaaaactcat gaaactcttt aatatgacca actttgatcg tgatgaatct
    15301 gtcatgaaat ttgcccgtca gcttcgtggt atgggggttg atgaagccct tcgtgcgcgt
    15361 ggagctaaag atggggattt ggtccgcatt ggtaaatttg agtttgaatt tgtagactag
    15421 gagactggta tgggagataa accgatatct ttccgagatg cggatggtaa ttttgtttcc
    15481 gccgcagacg tttggaatga aaagaaattg gaagaactat ttaatcgtct caatccaaat
    15541 cgtgccttga gattggcacg aactaaaaag gaaaatccat ctcagtaaag aagctaaaaa
    15601 atcccgtgcc tcatcagaca cgggattttg tggtacgaca ggcatgtata gcaaactgaa
    15661 tctggaatag cacagcatat cttctaaaat attgttataa attgatttga atttcaaaat
    15721 taaattgttt gattcttatc tcaatttgtt atagtatatc tgatgtcaaa gttctcggcg
    15781 agtcaaatag cgattcccaa gcctgactat cgtgaggtag cggattaaaa tggtctgggg
    15841 atagaccgtt ttaagtctga cgctggaaat aagaattgtc agaagaaggg atagcgaaat
    15901 cgtggctcta cgaacaggaa cgtgataata aggcgtatat agcggataag agggcctcaa
    15961 actctaaagt ccaaaaaggt agtcgtaacc tatatgcgta aatcacgaga gtaattgaat
    16021 tcgtactaag attttctatt ttcactgtaa gcttttaacg tccttatatc ttgtatacac
    16081 gaggaaagat gtacgactta tcccgtgagg tctatcacta taaagagaaa acgacagata
    16141 gaagtgatcc tgagtcacgg ttatctttaa aatagattgt ctgataggac ggtatgtata
    16201 aaacgcttct gtgaactgag agaaggggga gaagttcttg ctaaaattta gttgaacagc
    16261 cgtattccga tacttagata agagatctag tcttagctcc tactcagttt taggggataa
    16321 aaaaggggaa atagcgattc gagaaagatt atactcttcg aaaatctctt caaatcacgt
    16381 caatatcgcc ttgccgtatg tgtaggatac tgactacgtc agttccatct acaacctcaa
    16441 aacagtgttt tgagcaacct gcggctagtt tcctagtttg atctttgatt ttcattgagt
    16501 attagtaatt cagttactaa ctcgtcaact ctgatttatc caataaaatt gaaaaggatg
    16561 gaaaaaagga taaatttatg atatacttta ttttgaagac cttattagaa atcttgaaag
    16621 agtattgaaa acttagaatg agaaaaattg ttatcaatgg tggattacca ctgcaaggtg
    16681 aaatcactat tagtggtgct aaaaatagtg tcgttgcctt aattccagct attatcttgg
    16741 ctgatgatgt ggtgactttg gattgcgttc cagatatttc ggatgtagcc agtcttgtcg
    16801 aaatcatgga attgatggga gctactgtta agcgttatga cgatgtattg gagattgacc
    16861 caagaggtgt tcaaaatatt ccaatgcctt atggtaaaat taacagtctt cgtgcatctt
    16921 actattttta tgggagcctc ttaggccgtt ttggtgaagc gacagttggt ctaccgggag
    16981 gatgtgatct tggtcctcgt ccgattgact tacaccttaa ggcgtttgaa gctatgggtg
    17041 ccactgctag ctacgaggga gataacatga agttatctgc taaagataca ggacttcatg
    17101 gtgcaagtat ttacatggat acggttagtg tgggagcaac gattaatacg atgattgctg
    17161 cggttaaagc aaatggtcgt actattattg aaaatgcagc ccgtgaacct gagattattg
    17221 atgtagctac tctcttgaat aatatgggtg cccatatccg tggggcagga actaatatca
    17281 tcattattga tggtgttgaa agattacatg ggacacgtca tcaggtgatt ccagaccgta
    17341 ttgaagctgg aacatatata tctttagctg ctgcagttgg taaaggaatt cgtataaata
    17401 atgttcttta cgaacacctg gaagggttta ttgctaagtt ggaagaaatg ggagtgagaa
    17461 tgactgtatc tgaggacagc atttttgtcg aggaacagtc taatttgaaa gcaatcaata
    17521 ttaagacagc tccttaccca ggctttgcaa ctgatttgca acaaccgctt acccctcttt
    17581 tactaagagc gaatggtcgt ggtacaattg tcgatacgat ttacgaaaaa cgtgtaaatc
    17641 atgtttttga actagcaaag atggatgcgg atatttcgac aacaaatggt catattttgt
    17701 acacgggtgg acgtgattta cgtggggcca gtgttaaagc gaccgactta agagctgggg
    17761 ctgcactagt cattgctggg cttatggctg aaggtaaaac tgaaattacc aatatcgagt
    17821 ttatcttacg tggttattct gatattatcg aaaaattacg taatttagga gcggatatta
    17881 gacttgttga ggattaaacc gtagaggtgt ttatgaatat ttggaccaaa ttagcaatgt
    17941 tttctttttt tgaaacggat cgcttgtatt tgcgtccttt cttttttagt gatagtcagg
    18001 acttccgcga gatagcttca aatccagaaa atcttcaatt tattttccca acgcaggcaa
    18061 gtctggaaga aagtcaatat gcactggcca attactttat gaagtcccct ttgggagtgt
    18121 gggcaatttg tgaccagaaa aatcaacaaa tgattggttc tattaaattt gagaagttag
    18181 atgaaatcaa aaaagaagct gagcttggct attttttgag aaaagatgct tggtcgcaag
    18241 gatttatgac agaggttgtt agaaaaattt gtcagctttc ttttgaggaa tttggcttaa
    18301 aacaattatt tatcattacc caccttgaaa atgaagctag ccaaagagtt gctcttaagt
    18361 ctggatttag tttgttccgt cagtttaagg gaagtgatcg ttacacaaga aaaatgcggg
    18421 attatcttga atttcggtat gtaaaaggag agttcaatga gtaagcatca ggaaattcta
    18481 agctatttgg aggaattacc agtaggtaaa agggtcagtg ttcgtagcat ttcgaatcat
    18541 ctaggagtta gtgatggaac agcctatcga gctattaaag aagctgaaaa ccgtggaatt
    18601 gtggagaccc gtcctagaag tggaacaatt cgtgttaaat cccagaaagt tgctatagat
    18661 agattaacgt ttgctgaaat tgcagaagtg acttcttctg aggttctggc tgggcaagaa
    18721 ggtttagaga gagaatttag taagttttca attggtgcca tgactgaaca aaatatcttg
    18781 tcttaccttc atgatggggg gctcttgatt gtcggagacc gaacccgtat tcagttgcta
    18841 gccttggaaa atgaaaatgc agttctggtt acagggggat ttcaggttca tgatgatgtg
    18901 cttaaactgg ccaatcaaaa agggattcct gttctaagaa gtaagcatga tacctttacc
    18961 gtcgctacca tgatcaataa agccttgtca aatgtccaaa tcaagactga tattctgaca
    19021 gttgagaaac tttatcgccc tagtcatgag tatggttttc tgagagagac agatacagtt
    19081 aaagattatt tggacttggt tcgtaagaat cgtagcagcc gtttccctgt tatcaatcaa
    19141 catcaggtcg ttgttggcgt tgtaaccatg agagacgctg gtgataaatc accaagcacg
    19201 acaattgata aggttatgtc tcgtagtcta tttttggttg gattatcgac aaatattgcc
    19261 aatgtgagtc aacggatgat cgcagaagac tttgaaatgg taccagttgt tcgaagcaat
    19321 caaactttgc ttggcgttgt gacgcgacga gatgtcatgg agaagatgag ccgttcccaa
    19381 gtttcggctc taccaacttt ttctgagcag attggacaaa agctctctta tcaccatgat
    19441 gaagtagtca ttacagtgga accctttatg ctagaaaaaa atggagtttt ggctaatggt
    19501 gtattggcag aaattctgac ccacatgacc caagatttag ttgttaatag tggtcgcaat
    19561 ctcattatcg agcagatgct gatctacttt ttgcaggctg ttcagataga tgatatattg
    19621 cgcattcagg cacggattat tcatcatacg agacggtcag ctataattga ttacgatatt
    19681 tatcatggtc accagattgt ttcaaaagca aatgtgactg ttaaaattaa ttagaaacta
    19741 ggagaaaaga tgataacatt aaaatcagct cgtgaaatcg aagctatgga caaggctggt
    19801 gattttctag caagtattca tataggctta cgtgatttga ttaagccagg cgtagatatg
    19861 tgggaagttg aagaatatgt ccgccgtcgt tgtaaagaag aaaatttcct tccacttcag
    19921 attggggttg acggtgccat gatggactat ccttatgcta cctgttgctc tcttaacgat
    19981 gaagtggctc acgctttccc tcgtcattat atcttgaaag atggtgattt gctcaaagtt
    20041 gatatggttt tgggaggtcc cattgctaaa tccgacctga atgtctcaaa attaaacttc
    20101 aacaatgttg aacaaatgaa aaaatacact cagagctatt ctggtggttt agcagactca
    20161 tgttgggctt atgctgttgg tacaccgtcc gaagaagtca aaaacttgat ggatgtaacc
    20221 aaagaagcta tgtacaaggg tattgagcaa gctgttgttg gaaatcgtat cggtgatatc
    20281 ggtgcggcta ttcaagaata cgctgaaagt cgtggttacg gtgtagtgcg tgatttggtt
    20341 ggtcatggtg ttggcccaac tatgcacgaa gaaccaatgg ttcctaacta tggtattgca
    20401 ggtcgtggac tccgtcttcg tgaaggaatg gtcttaacca ttgaaccaat gatcaataca
    20461 ggcgattggg aaattgatac agatatgaaa actggttggg cgcataagac cattgacggt
    20521 ggattgtcat gtcagtatga acaccaattt gtcattacga aagatggacc tgttatcttg
    20581 actagccaag gtgaagaagg aacttattaa taaaaagtga aaagactact ggaagtttat
    20641 tttgataaaa aatccagtag atcttttcat aataaaacgc attgtatcaa gtgttagggg
    20701 ctgatatcat gcgtttttct gcttttaaga ttttttccaa ctttgtttgt aagcgcatca
    20761 taacaaaggg tctaggattc agggctctcc tcctatatac tattagtaaa gtaaaactaa
    20821 gggaggatat tttagtgtcg cagtctattg ttcctgtaga gattccacaa tattgtcgtt
    20881 ttgattctaa aaagag
//
LOCUS       DAVLXF010000027        20764 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLXF010000027 DAVLXF010000000
VERSION     DAVLXF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.027"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..1448)
                     /gene="pfbA"
                     /locus_tag="VYO12_000948"
     CDS             complement(<1..1448)
                     /gene="pfbA"
                     /locus_tag="VYO12_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1587586.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEY"
     gene            complement(2612..3838)
                     /locus_tag="VYO12_000949"
     CDS             complement(2612..3838)
                     /locus_tag="VYO12_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1587587.1"
                     /translation="MPNYNIPFSPPDITEAEITEVVDTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSIYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAASRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(3881..4573)
                     /locus_tag="VYO12_000950"
     CDS             complement(3881..4573)
                     /locus_tag="VYO12_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1587588.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(4746..6497)
                     /locus_tag="VYO12_000951"
     CDS             complement(4746..6497)
                     /locus_tag="VYO12_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587589.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATMVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQETSDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGV
                     TYHKMYSLQAGAMADTL"
     gene            complement(6487..8232)
                     /locus_tag="VYO12_000952"
     CDS             complement(6487..8232)
                     /locus_tag="VYO12_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587590.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLLAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGTI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(8438..8734)
                     /locus_tag="VYO12_000953"
     CDS             complement(8438..8734)
                     /locus_tag="VYO12_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1587591.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(8887..9344)
                     /locus_tag="VYO12_000954"
                     /pseudo
     CDS             complement(8887..9344)
                     /locus_tag="VYO12_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            9478..10305
                     /gene="xth"
                     /locus_tag="VYO12_000955"
     CDS             9478..10305
                     /gene="xth"
                     /locus_tag="VYO12_000955"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1587592.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            10317..10454
                     /locus_tag="VYO12_000956"
     CDS             10317..10454
                     /locus_tag="VYO12_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1587593.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLLKSV
                     N"
     gene            10565..10693
                     /locus_tag="VYO12_000957"
     CDS             10565..10693
                     /locus_tag="VYO12_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV1587594.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      11076..11171
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11571..12152
                     /locus_tag="VYO12_000958"
     CDS             11571..12152
                     /locus_tag="VYO12_000958"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1587595.1"
                     /translation="MKLLEDRILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            12152..13414
                     /locus_tag="VYO12_000959"
     CDS             12152..13414
                     /locus_tag="VYO12_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1587596.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLRVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPLDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTRFSQNVGLVKLSGIKKHLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(13465..13926)
                     /locus_tag="VYO12_000960"
     CDS             complement(13465..13926)
                     /locus_tag="VYO12_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV1587597.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(13923..14687)
                     /locus_tag="VYO12_000961"
     CDS             complement(13923..14687)
                     /locus_tag="VYO12_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV1587598.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(14868..15272)
                     /locus_tag="VYO12_000962"
     CDS             complement(14868..15272)
                     /locus_tag="VYO12_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1587599.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(15342..16823)
                     /locus_tag="VYO12_000963"
     CDS             complement(15342..16823)
                     /locus_tag="VYO12_000963"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1587600.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(16842..18020)
                     /locus_tag="VYO12_000964"
     CDS             complement(16842..18020)
                     /locus_tag="VYO12_000964"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1587601.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            18185..19192
                     /gene="galR"
                     /locus_tag="VYO12_000965"
     CDS             18185..19192
                     /gene="galR"
                     /locus_tag="VYO12_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1587602.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(19238..20275)
                     /locus_tag="VYO12_000966"
     CDS             complement(19238..20275)
                     /locus_tag="VYO12_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1587603.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(20276..20629)
                     /locus_tag="VYO12_000967"
     CDS             complement(20276..20629)
                     /locus_tag="VYO12_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1587604.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
BASE COUNT         6104 a         4646 c         3918 g         6096 t
ORIGIN      
        1 aaatattctg cactatcttt tgcaactcgt atcgcttttg ttttaggatc ggcaatatta
       61 aatatatttt cggcgataac cacctgttta tttaaatcta ataggtcttt agtgttttta
      121 tagctataag catttactaa agctgctccg ctttctcgat aatgtacact ctctccttta
      181 actttcttat caaagcgatt tcctttgatt aatacatcag tgaatcctgt aaaacgtaca
      241 cctgcataca tcatgttatc aaaatgatta tttaaaattt taatattaga ggggttctgt
      301 gtcgacaatg tttgatagtg tgtgccaatt gctgttacta attccccaga tttatcactt
      361 ttgccaaaat aggaattttg aatagtcaca ttttcagatt ttttcccatc atcattcaag
      421 gcataaggaa aaccttttct agttaatggt tcaatctgaa tgctctcctt acttatgatt
      481 tgcccatcct tcatcgtttt gggtaaggct tgcccaagaa aacgagaatt atcaactaat
      541 acatttttcg aacctgcaat ttgaatagca tgcccttgat aactatcctt gaatgttaca
      601 ttttttatag ttacgttatt tgaattccca atagcaaatg cacctgaaga atttataagt
      661 ggtagatttt ttgctttagt tccttcttca ttcaaagcac cgttcatatc aatcgtacca
      721 ccagaataac taatatcttc tgttgggccc cattctactt gcgcaccatc atccgtaaat
      781 aaacctgtca taaaaacaat ggaagggtga ttcttaatat ttataccatt tagaattgta
      841 gctttctcat tcaattctaa gtgtgtatga ctttttaaaa aaacaatttc ttttactaaa
      901 taagttcctt caggaaaata tacatttcct ccacctagcc cttgagctgc agcatctatt
      961 gcatcttgaa ttgcttgtcg atcatcatga atcccatcac ctaccgcacc ataatctttt
     1021 acacttaaag cagtatcttc aaattctttt ttgataattc tgtcacttat aacatcttcc
     1081 ttataactct tattttcatt agtagatgtt actgtagatt caactgttct gttgtctaat
     1141 tgtgaattgg aacgaatagt accattagag ctgatgctat taacacttaa cgaattatct
     1201 aaaccattag tggttgaatt agaggcaatc atttcagcag aacgattctg attaacagtt
     1261 ggtgaattat ctaaatcatt agtaattgca ttagaagact cttttgtagc catcggtgaa
     1321 gaactagtaa caacttcatc cgataaaata ccttggcttc ccaaaattga aattgccaat
     1381 agtaccgagc aagtccccac ctttaattta cgaatactga atacctcatt aggaacaaaa
     1441 tatttcatca gaacctccaa tttttttact atttttaaca attgattatt acttttctta
     1501 aaatagatag aatgacaccg ctgtcattat ctttatcata tattttccaa attgaattgt
     1561 caaggaataa cacaagaaaa tattaaaatt tatactcttc ggaaatctct tcaaactagg
     1621 tcagtgccgc cttgccctaa gtctaggtta ctgacttggt cagttctata gtagactgaa
     1681 actataatag tacacctcta cttctaaaac attgttagaa atcgatttga ctatcccgat
     1741 cgatttgtcc tattcttatt tccttttact atatctacaa cctcaaagca gggttttgag
     1801 ctatctatgg ccaacttcct agtttgctct ttagccttcc attcaaaagt gatgctatct
     1861 ttcttagctt gaatataaaa tatcccattt actatattta aatttattga tatgttgtaa
     1921 attataaatg aaagaacaaa taccttgtca tacaagcatt cgccagggat atttacctta
     1981 atatcgctca tatggcggat tgaaactaaa acactacaat atatttctta aatagacctt
     2041 ctataaaagc tagatttaac ctcgataaaa atagatgcag tgcttctaaa atactggaat
     2101 agggcatata ggtaatcgta gacaagtgtt ttatagattg tctcgcagga gatctaatga
     2161 tgtatagcaa gcatcactaa acatatggga tatttgcgtt attataatat caacatgcta
     2221 taaattctat catgcaaaag aatgactaga acatgaaaga caggcacaaa actttgaaca
     2281 atattctgaa aacaaaaaaa agacgctagt ttccacaatc tcactcatgg aaactcacgt
     2341 ctttcaaaaa atagggtgaa ttgaaaacta gaacaagaca ctagattttc taaatgatgt
     2401 ctagtaagga ttctaaatgc tataccaaat tgctcacatc ttatttcaaa ccacgggact
     2461 cccacaagaa aggaaaatcc tataaaaatt ctctctctga ggagaaagag gcctcaacta
     2521 caggtgtaaa ctattatcaa ttgaaaacaa cagtttttac actcaattag actttttagg
     2581 ggcaggatca ctttcgcttg actgtagttt gtcatttttt tgataaagtt agcacttttt
     2641 cagaaactgt tttgaaagtc tcaatgatat agtctacttc ttcatcgctt aatttagtat
     2701 gaagagggag ggtaatttca ttctcaaaga aggcataggc cttaggatag ttcgtcatat
     2761 caaatccaag attcttatag gctgtcaaga gaggaagcgg tttgtagtga acattacttg
     2821 caattcctgc tttagccaat tcttggatga tgaggttgcg ttcttctagg cttgctcctt
     2881 ctacacgggt gatgtagagg tggcgtgaag attcgacagt ttcagtcttg tgtgccaaag
     2941 gatggatgcg agaagctgca aaaccactat catagcggtc cacaatgtcc ttacggcgtt
     3001 gcaacaaact tggatagcgg tccaattgta ccaaaccaag tgaagccatg atatcggtca
     3061 tgttgcactt ataggctggt gtaacgatat cgtattccca tgaccccagt tgcatcttgg
     3121 caagagcatc cttagtttgc ccgtgaaggg aaaggatttg gaattccttg tacatctctt
     3181 cgtcatcaat cactggattg gctttccaag tcgcacttcc accttctgcc gttgtaaagt
     3241 tcttaactgc atggaatgag aaggaagtaa agtcagcgat agaaccagaa ggttgtcctt
     3301 tataaataga tcccaaagcg tgggcactat cagagacaat gacaatacgg ttaaaggcct
     3361 tttgccactt gcttgaagcg gtaaagaagt cacgtttttt ctccacgact tggaacaaac
     3421 ggtcataatc gcaaacaatc cctgcgagct ctactggaat aatcacctta gttttctcag
     3481 tgatagcttg ctcaagcagg tcatagtcca tctcaaacgt atctgcttgg atatccacca
     3541 tgacaggggt tgctcccacg tgcgtaatga cactacatga agccgtatag gtcatggctg
     3601 gaacgatgac ttcatcacca ggtcccactt ccaaaacgcg taaaatcaac tccagagcgg
     3661 ctgtcgcaga gttgagacaa acagtcttag gtgtctgtgt gtaaagagac aagcggcgct
     3721 ccagttcttt tgttttagga cctgttgtga tccaaccaga acgcagggta tccactactt
     3781 cagtaatttc tgcttctgtg atatcaggcg gtgaaaatgg aatattgtaa tttggcattg
     3841 atttgctcct tttatctgta ctcattttct tatgactact ttattttagt acctcaaaca
     3901 cggtttgaaa catgattttg atgtccccaa agaaactaaa ctctcggaga taggcgaggt
     3961 tatagcgcat cttttcagga agaacatgct ccacataggc ctgatcaact gacagacctt
     4021 tctccgtcat ttgactgata attgtgtcct catccttgta gttgatgctg gctggagagg
     4081 taatccctgc ttgcaagagc aaggttgcca tcatttcagg gctatactgc tctgtataac
     4141 gtggcacttc aggtcgtgta ccgacaaagg acatctcacc tttaaggaca ttgaccaact
     4201 gaggcagttc gtccaaacgg acacgtcgga tgaaatttcc aaccttggta atgcggctat
     4261 cgttagcaga agtcaccaga cttccttttt tatccgcatc cgtcaccatg gtacgaaact
     4321 tccaaatctt gaaccgacgg ttgtactggg tcacacgctc ttgcttgtaa atcactggcc
     4381 ctttgctatc caacttgatc caaatgctca agatgagaaa gatgggagag gtcagaacca
     4441 gtaagaccaa ggccaaaacc cagtccaagc aacgcttgaa aatcagcgaa cccttccttt
     4501 tagagacaag ctggtagtaa gactcaacct cgcttgattt catttccaca ggcaagtctt
     4561 cccatttcag catgctgttc tcctttgttt ttattatact atttgtcaac atttttcatt
     4621 ataccacaaa atggaaaagg cggtgaaaga aatctgtaat ttgaaggatt tccttctagt
     4681 actcaatgaa aatcaaagta cagctttgag attgcagata aagctgacat ggtttaaaga
     4741 gattttcaaa gagtatcggc catggcccct gcctgcaaac tatacatctt gtgataggtt
     4801 actcccagag ccaagagttc ctcatgggtt ccactctcga taatgcgtcc cttatccaag
     4861 acatagatgc agttggcatc ttgaatagta gaaaggcggt gagcgatagc aatagttgtt
     4921 cggccctgtc tcatcttcgc cagagaagct tgaaccaagc tttctgtttc agagtcaata
     4981 ttggctgtcg cttcatccaa aatcaggatt ttaggctggc tggcgactgt tctagcaaag
     5041 gcaagaagct ggcgttgccc agtagagaag ctcgaaccac gctcggaaac aggggagtcg
     5101 tacccctgag gaagttcttg aataaaggaa tctgcatcca caaaggctgc cgcagcctga
     5161 acctgctcat cactggtttc ttggtacatg gcgatattgg acttaatagt tccatgatag
     5221 aggaagggtt cctgcaagac caaaccgatg ttttttctca gctcttcttg actgaaatcc
     5281 ctgatatcca catcatccaa gagaactctc cctgactgga attcataaaa gcgcatgagg
     5341 acattgataa tagacgattt ccctgaacct gtatgaccta caaaggcaat ggtttcaccc
     5401 ttattaacag agaaagaaat gtcatccaga atcggatgtt taccgtcata tgagaaacac
     5461 acatgttcaa aacggatatt gccttcttgg actttggctt gcccattttc ttgaagaggt
     5521 tcataggtcc tctcgtctat cagggcaaag acacgacctg cagaaaccat agccgtttgc
     5581 agagttgaaa agttttgcgt cacctcaatc aaggggtcaa aaaggcggtt gatgtactgg
     5641 ataaaggcat acatggtccc gaccgttatc ccgatagaaa aaccacggta gccaaagtag
     5701 gccatcaaga ctgcatagcc tagaagtttc agcaaactca tggcaggtct caaaaagagg
     5761 gcatccaagg ctacagaacg gttggcgtag accaagtgtt cttggttgat ttcatcaaat
     5821 tctgcctgca ggcgcttctc ttgattaaag gcctgaataa tcctgattcc ctcgatattc
     5881 tctgccagct tactattgat atctgacaag agacttctgg ttttctcgat gattttcact
     5941 gacttttttc gatagagatt gaccaaaagg aaaatcaaag gaagaaagag caagactaaa
     6001 gccgtcaaac gaaaatccag caccaacatg gtataaaggg ttgtcagaaa gataaaaact
     6061 gctgagataa agctggataa aatcccagaa aacatatcac taatcgtctc ggtatcgttg
     6121 gtcaaacgag aaacgataga acctgctggc gtcttgtcaa agtaagacat gcccagtttc
     6181 tccatattgg caaaggcatc ccgacgaata tccctaacaa tactgtaaga cacgcgcgca
     6241 aagagaagat tgcctacata ctgaactaca gtttgtagga tgtagagacc atagtagacc
     6301 agcaaaacgg taacggctag ttggttaaga ttgctgagat actggtcgat aaagtgggaa
     6361 gccacgaggg gtatgacact tttaatgacc atcgtcgcta ggagaaaact gagtgccaaa
     6421 aaggtcagga gtccataagg cttgagataa gacatcaagc gcttcaatac agcccattgt
     6481 tcttgtttat tctgcatctt cttctccttt catttccaac tgctgagact ggtaggtttg
     6541 ggcataccag ccatccatcg ctagcaagtc ttcgtgcgtg cctcgttcga taatttgacc
     6601 attttgtaga actaaaataa aatctgcatg gacaacagca ctgaggcgat gggcagtgat
     6661 aatggttgtc ttgtcctttc gcgtctcctt gaggttgtcg ataatcgcat actctgtctt
     6721 ggcatctacg gcggataagg aatcatccaa aatcaagata tcagggtcta aaatcatagc
     6781 ccgactcata gccaaccgtt gcttttgacc accagaaaga ctgactcctt tttcaccaat
     6841 cagcgtatca aatccttgag gcatgtctac aatatcttgg taaactcggg ctagcttagt
     6901 agcttcctcg accgctgaaa ggggcaagtt aggattgcca aagcggatat tgtctaggat
     6961 tgaagtcgca aagagaaact ggtcctgagg aacatagccc atgagactgc gaaggtctgt
     7021 cagacgatag tcccgaatat cgtgaccgtt tagataaata gtacccttat ccacatcgta
     7081 ttcacgcaag aggagcttga ttaaggacgt tttcccagag cctgtctgcc caaccaagcc
     7141 cagtgtttgc ccttttgcca aactaaagtg aatatccgtc agtgtttcct cattttcaaa
     7201 agcaaagctg tcaatggcat actccaaacg cccattttca ataccatcca gaggaaactc
     7261 agggtcttgt acaggagatt cctgagacaa aagattttca atccgctggt aagaaacctt
     7321 ccctcgctga gtagtattaa agaggaaacc gatggccaga agaggccaga ccagcatatc
     7381 caaatagctg ataaaggtga ctagattccc aactgtaatc tgcccttcct gaaccatcaa
     7441 ggagccaacc aaaagcgtta aaacatagga cgaaccaaca aacaagagaa ccatagggtc
     7501 aaagagacta tcatatttca tggtttgcag gttcttttgg aaggttaatt cattgactgc
     7561 ctgaaaagac ttcaactcgt ctgcctgata accgaaagac ttggtcactt tgatacctga
     7621 tacggactcc tgtaccttgt tattgagttc agaaaaagca gcttgggatt cgccaaaggc
     7681 cttatgagtc tttctcccta ggcgactagt cgtataggcc atgaaaggta gggggagaat
     7741 ggcaacaaga gtcatctgcc atgagatgct aaagagcatg gtcaacaaag tcaccagagc
     7801 cgtgatagag gcatccaccg cagacatgac accgccacct gctaaacgag tcaaggcatt
     7861 gatatcattg gttgcgtgtg ccatcagatc acccgtccga taggtttgat aaaaggctga
     7921 cgacattttt gtgaaatgct taaacaagcg agaccgcatg atctgtccca agcaataaga
     7981 ggtcccaagg atatacatac gccacacata gcgcaaatag tacataccaa aggctgcaag
     8041 tagcaagtaa aataggctaa gaaggaggtc ctgctgagtt aattgccccg atgtgatggc
     8101 atcaatgact cgccccataa ccataggagg aatgagattg aggacggaaa ccaagaccag
     8161 ggccacaatc ccgactagat aacggcgttt ttctaacttg aaaaaccacc agagtttttg
     8221 aataatggac ataaaatccc tttctgattg caaatggaaa cctgaggcca atactcaatg
     8281 aaaatcaaag agcaaactag gaaactagcc gcaggctgct caaagcactg ctttgaggtt
     8341 gtagatagaa ctgacgaagt cagtaaccta catacggcaa ggcgacgttg acgcggtttg
     8401 aagaaatttt cgaagagtac aagaccccag gtttttctta ttcataagtt acgactgtaa
     8461 cagcaccctt gtcatattca gcaataaaga tattggctac attgtcatgc ccttgtttac
     8521 tgaggttatc aagcaaccac tcttcgctac gaccaatcga ttccaagaca tctacttgaa
     8581 tcacaccgtc tgtcacaact ggatacttag gattttcatc tcccatttgc acaacgatga
     8641 gttgcccatt ttgctcttgc acagctcgtt tgacttgctt catctggaaa atcccttggc
     8701 tacgaagttt gagggctaca tccgatgcag acaaaccaac tgaacgacag gcttatgggt
     8761 caatattccc atttttgata aggagagttg gcttaccatc aatcaaacgt ttcacaaagt
     8821 gaacattgtt gttgagctat ttgaaagtca agatcagttt gattaaaatt tcgatataat
     8881 taagtgtcat ttgttcacat ccaccagttc aattgcgtct gtcttataca attcgacttt
     8941 ctctaacagg tacttgtctg gctcacttcc gtttagggca cgataatagc gatctcccac
     9001 cttgataagt gcgccatctg tgtttgtgga agtattaaca tagacttctg acttgtctac
     9061 tcccaaatct ttggaaacaa cctcgatgaa atgaagtgaa gtttgaaatt tattgttaga
     9121 aacttgattg ttttgataag tcgtgatgct gaccaaaagc agagctaaat aaggccagag
     9181 ctgaaatcat gaccaactca cgaaatttgg ttcctttttt atcatggtat gccttgaaag
     9241 caaaaaaaaa ctgtcacagc taatagaaca atcccaaagc caaccatgat accattttgc
     9301 tgaccgattt ggctgagtac atagtcataa gagtagaatt tcatgtattt tcactccctt
     9361 tcataaaatc attcttaatt ataaacgaaa gagagtaatt tttcaaacca tacgttgggg
     9421 aaatagatct ttttttggta taataaaatc tataatctga atgaaaaagg taactttatg
     9481 aaacttatct catggaatat tgattcccta aacgctgccc taactagtga ctcagctcgt
     9541 gccaaattgt cccaagaagt cctacaaacc ttggtcgctg aaaatgctga tatcattgct
     9601 atccaagaaa ccaagctttc tgccaaagga cctacaaaga aacacgtgga aattttagaa
     9661 gaactcttcc caggctacga aaacacgtgg cgctcttccc aagagcctgc ccgtaaaggc
     9721 tatgctggaa ccatgttcct ttataagaaa gaacttacac ccactgtcac tttcccagaa
     9781 atcggtgccc cttctaccat ggacttggaa ggccgcatca tcactctaga atttgatgca
     9841 tttttcgtaa cccaagttta cactccaaac gctggtgacg gtctcaaacg cttggaagaa
     9901 cgccaagtct gggatgttaa atatgctgag tatttggctg aactagacaa agaaaaacca
     9961 gtccttgcga ccggtgacta caacgtagcc cacaatgaaa tcgaccttgc aaatcctgct
    10021 agcaaccgcc gttcacctgg atttactgac gaagaacgtg ctggatttac caaccttttg
    10081 gcaactggat ttacagacac cttccgccat gttcatggcg atgttcctga acgctacact
    10141 tggtgggcac aacgcagcaa aacttctaaa atcaacaata caggctggag aatcgactac
    10201 tggctcacaa gtaaccgtat cgctgacaag gtcactaaat ctgatatgat tgactcaggt
    10261 gctcgccaag accatacacc gattgtcttg gaaattgact tgtaaggatt ttctatatgg
    10321 actacaatgc ggtcattccc gagttgcttg tatcgaatat cgaacagtcc cggtccttct
    10381 actgtggttt actgggtttt aggattgaat accaacgtcc agaagaaaac tttctcttga
    10441 agagtgtcaa ctaatgttag aggagggaac aaaggatcaa ctagctgaac tgacctaccc
    10501 ctttggtcgt ggagtcaatc tctcctttgg cataaaggat gttccaaaac tctattaaag
    10561 agtgatgaag gcaaactatc ctatttatcg tcccttgacc aaaagaaagt ttcgtgtcag
    10621 tgatccctat atctatcctc ataaatttgc agttttggat ccagatggct attttttaag
    10681 atttagcgag tagaataata aaaattggga aaagtcttta aagttagaaa aaggcatatt
    10741 gtcaggtgtc taaaaacttg atagtatgcc ttttatgatg gaaaaatctt ctaatactct
    10801 tcgaaaatct cttcaagcca cgtcaacgtc gccttaccgt aggtatatga tactgactct
    10861 gtcaattcta tccacaacct caaaacagtg ttttgaggtt gtggatagct tcctagtttg
    10921 ctctttgatt ttcatttagt ataaatgtta gcttacaatg gagtttttgt tcaccccctc
    10981 ttctgtttgt tcgtttttta aatcaaaaca ctaacaaaat agttgattta taaagttatt
    11041 tagtgtcaaa taaaaacatt ggctcaagcc tatttaaaat tgaatatcgt tttacttgtt
    11101 tatgtcgtga attggcacga cgtttctaca aggtgccgga acacctaaca ataagtaagt
    11161 cagcagtgag atatggtcgt ttttgccatg cctatttggg ggacttactt agagattaag
    11221 taggtccttt ttgaatattt ttaatatttc ccttaattag tgcggacggg aggtgaaatt
    11281 atcctgtgaa taatttcagg aattcaggga atttcatccc taatttttga gcccctagct
    11341 ctttcatttc taggcttagg ctaaaatagt tccccgaact attttactct caaaaatccc
    11401 gtcatagaca ataacaagat tattgtctat gataccacta catcgggaaa tatttgataa
    11461 gactattacg agcgaagcga gtcttatcaa atatatagta ttagcaaaag ggatcactat
    11521 agaaacattt caacaacaag taaattagct tttagacttt aggaggtctt atgaaattat
    11581 tagaagatcg catcctcaag gatgggcata tcttgggtga taacatcctc aaggtagatt
    11641 cctttttaac ccaccaagtt gactttagct tgatgcgaga gattggtaag gtttttgcgg
    11701 aaaaatttgc tgctgccggc attaccaagg tcgtaaccat tgaagcgtcg ggtattgccc
    11761 cagccgtttt tacagctgaa gccttaaacg ttcccatgat tttcgccaaa aaagctaaga
    11821 acatcaccat gaacgaaggc atcttaactg ctcaagtcta ctcctttacc aagcaggtga
    11881 ccagcactgt ttctatcgct ggaaaattcc tctcaccaga ggacaaggtt ttgattatcg
    11941 acgatttcct tgctaatggc caagctgcta aaggcttgat tcaaatcatc gaacaggccg
    12001 gtgccacagt ccaagctatc ggtatcgtga ttgagaaatc cttccaagat ggtcgtgatt
    12061 tgcttgaaaa agcaggctac cctgtcctat cacttgctcg cttggatcgt tttgaaaatg
    12121 gtcaggtcgt atttaaggag gcagatctct aatgcaaact caagaaaaac actcgcaagc
    12181 agccgttctg ggcttgcagc acttactagc catgtactca ggatctatcc tggttcccat
    12241 catgattgcg acagcccttg gctattcagc tgagcagttg acctacctga tttctacaga
    12301 tatcttcatg tgtggggtgg caaccttcct ccaactccaa ctcaacaaat actttgggat
    12361 tggactccca gtcgttctta gagttgcatt ccagtcggtc gctcccttga ttatgattgg
    12421 gcaaagccat ggtagtggcg ctatgtttgg tgcccttatc gcatctggga tttacgtggt
    12481 tcttgtttca ggcatcttct caaaagtagc caatctcttc ccatctatcg taacaggatc
    12541 tgttattacc acgattggtt taaccttgat ccctgtcgct attggaaata tgggaaataa
    12601 cgttccagag ccaactggtc aaagtctctt gcttgcagct attactgttc tgattatcct
    12661 cttgatcaac atctttacca aaggatttat caagtctatc tctattttga ttggtctggt
    12721 tgttggaact gccattgctg ctactatggg cttggtggac ttctctcctg ttgcggtagc
    12781 tccacttgtc catgtcccaa ctccactcta ctttgggatg ccaacctttg aaatctcatc
    12841 tattgtcatg atgtgtatta tcgcaacggt gtctatggtt gagtcaactg gtgtttatct
    12901 agccttgtct gatatcacaa aagatccact cgacagcacg cgccttcgca acggttaccg
    12961 cgcagaaggt ttggccgtac ttctcggagg aatctttaac accttccctt acaccagatt
    13021 ttcacaaaac gttggtttgg ttaaattgtc aggcatcaaa aaacacctgc caatctacta
    13081 cgcagctggt ttcctggttc tccttggact gcttcctaag tttggtgccc ttgcccaaat
    13141 cattccaagc tccgtcctcg gcggtgccat gctggtgatg tttggttttg tatctattca
    13201 agggatgcaa atcctcgccc gagttgactt tgctaacaat gaacacaact tccttatcgc
    13261 agctgtttca atcgctgcag gtgtcggtct caacaacagt aatctctttg tcagcatgcc
    13321 gacagccttc caaatgttct tctcaaacgg aatcgtcgta gccagcctac tcgccattgt
    13381 cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt
    13441 gtgtgacaaa ataaaaacat ctacctattc atatatgcta gtgtcatagt cctgaaaatc
    13501 atctgcatca agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc
    13561 atctggattt tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt
    13621 atactttttc agttttagat tgctttccaa taattcacct attaacaata atctatgata
    13681 ggagctatgt agccactctg gcaatttatc tcctacttcc cacccatatt tttccttcat
    13741 caaagctagt tgtttttcgt caccaaattt catattttca gataaccatc tcacacaatc
    13801 gggatctgat aaggtacaga cgattaaact taggttatcg taacggcttt cagtttcata
    13861 atcaggccag atgactttta agaaatcata cacctcttct gatgcaacaa gttgtttcgt
    13921 tttcataatt tccgatactt tcctctacct ttaaattcta ttatttcttt gtctcttaat
    13981 atttgaagct gttgcctaat cttttctttg atatgattgt tcttaacaaa gatatttttt
    14041 aggtcacttt caaaacgata catatcttca agggtaaatt ctgaaccctc tatcttatct
    14101 atacaattta gaatttctat tgtccaacct cttgatgaca gagagctctt ccttaaaaat
    14161 aaaccttgct taaattcttt tgtaactttt tctggatctc taacttgtcc atcttgcaca
    14221 agaaatatcc ttcctttaga aggtacttgt gataaatcaa tgttacaacc aatccaacct
    14281 gctcgtctag cagttggtgc aagtggtttt ctttgaataa tcgatttcgg tgtaacaaat
    14341 tgcttcggaa ggacaagaaa gttatttacc tcaaaatttt ttgtgtaagt taaaaaaaag
    14401 aaattaggat tattatctgc ctgcacacgc ttcatcatcg ttgcataagc accatcattg
    14461 attgttgatg aaaaatttcc ttttttgctc tttagttcaa actcctcact acaatgatta
    14521 cagtaaaaat ctgctacagg ccgattattt tcaaaatgat ttaagggatt gttcccacaa
    14581 ttagggcaat aagactgacg ataaacccaa tcctccgtta agatacgtgc cttttggcta
    14641 ttacttttat aggtttctac taattctaaa ttaaagtgta attccattta aaaattcctc
    14701 actaatatga gaactcactc gatataagca attaaaatct actataacta gtataccaca
    14761 tatatgtacc tattacattt gtatttcatc actctctttt atatttcctt ctaacatatc
    14821 tcataaatta aaaatgagtg agataaattt atcccactca cctttcttta tatacttacc
    14881 cgtctttcct cagacctttg gcctgtgaca aacatggtaa aggttgcttt acagacattt
    14941 ctgccctctt gattggtgat atccacatcc atcacacagg ttgtacgacc ttgatggaca
    15001 cattctcctt taatggtcaa tacatcgtcg agttttcctg ccttaaggta gttgatagag
    15061 gattggagtg tcaccccatc aagtcccagc gagataacca ccaaaccact gatttggtca
    15121 catagcgtga atagatagcc accatgggca ttgccatagt agttgagcga cgagttcact
    15181 actttggtcg tcaccacaac gtgaccatct ctcatttttt caatttcgta attttcaaag
    15241 gcagatatag cgtcaaaatg aaagtctttc atcgtttcct cctgatattt caaacgacac
    15301 tatgatacta ctttttatag gactgtgcaa ggagaaagct cctagtctag taaaattccg
    15361 acatgttcca caaagcgcat aaaggctgtc tgcccttgtt ctgtctgctt gtagactcct
    15421 gcatcctcaa gtacacgcgc aaagatagca cccacagagt ccttgacgat tgcaagggct
    15481 ttttctttat ccgttaggtc tggatgttgg gatttgagtt ggtctgccca ctcctgatga
    15541 taatcggcaa ctgtaacagc ttctcctaca agatagctcg cgacttgctc cacttcttct
    15601 ttcagacgtg gtggcaagat tgccaagccc atgacctcaa tcaagccgat attttccttc
    15661 ttgatatgtt ggacatcctt gtggggatga tagatgccat caggatgctc tgctgaagtc
    15721 tgattgtctc gcaagaccaa gtccaactca aactgtccat cacgcttacg cgctattggt
    15781 gtgatggtgt gatgcggtgt cccgtctgtc tctgctaaaa tctgcacact agaatctgaa
    15841 tactgacgcc attcctggaa aatcttatca gctaagttaa tcaaatcctc tttggaatcc
    15901 gaagtcaagc gtaggacaga cattggccac ttgataattc cagccttgac ctgctcaaaa
    15961 ccagcaaatc ggaaggtctt ttgcaaggga gccaattcca taggaaatac gtgacggcct
    16021 ccctgataat gatcgtgagt tagaatagag ccccccacaa tcggcaggtc ggcattagat
    16081 ccagcaaaat atcctggaaa ctgctctacg atagccaaca gacgttcaaa actctgacga
    16141 ctaatggcca tgggacgatg ctggccatct aaaaagatac aatgctcatt aaagtaagca
    16201 tagggcgaat actggaaccc ccattcctga ccaaccattt caaaacggat aatccggtgg
    16261 ttgctgcgag ctgggtggtt gacccgacca tggtaaccct cattctctaa acaaagttga
    16321 cactgaggat aattactagc ttgcaccaac ttggctaccg caatctcttt cggatccttt
    16381 tcaggcttag agagattgat ggtaatttca agttctccat aatcagatgg aacacgataa
    16441 gcgatatttt tagcaatggc cttgagtttg atgtaatcat ttttctgact gagttgataa
    16501 aaatcctcta tcgcttgctc aggagagtgg acataggctt cccagaagtc acgattgacc
    16561 tgactcggat aaggagtcac caagtccatc agttcagtac caaggatttc acgcgcagtc
    16621 tgactatcct caatcatctc taatcgaacg gcttcctcaa ctagctggtc cttgaggtca
    16681 atcaatttat ccagattggt ctcaacttcc aaaacacctt ctcccactcg tgccaaaaca
    16741 cggttggtca ggtagattcg atccatttcc tcaaatgaac tttcagaaat gacatgtgtt
    16801 acaaatttat ctactaagat cactatagag cctccttttg actagtcaag gacgcgagtg
    16861 ccacctgcaa cttcagcgat atagaagctt ggagcgtatc caactacttc ctcgtagtgt
    16921 ttgcctacag cttccttaaa ggcctcaaca gtatcttttt gaaccaaggc aatggcacag
    16981 ccaccaaaac cagcccctgt catacgagca ccgagaactc cttcttgtgc ccaagctgtg
    17041 tgaacaagag tatccaattc caaaccagtt acttcataat catgctccag agaaacgtgt
    17101 gacgcattca tcaaacgacc aaatgtttcc aaatcacctg cttgaagggc tgcttgagct
    17161 ttaagggtac gttggttttc aagcacagca tggcgagcac gtttcaaacg attttcatct
    17221 ttaatcaaat agctatattg gtcaacggcc cactcgtcca attcacccag ggtctgaata
    17281 tccaaggcaa cttgcaattc ttccactgct ttttcacact cagcacgacg ttcattgtat
    17341 ttagagtccg ccaattcacg gcgtttgttg gtgttcatga taacaacgac attgtccttc
    17401 aaatcaagtg gcaccaagtc gtattctaaa gtattggtat ctaagtaaat agcacgttgg
    17461 tcagccccca taccaatagc aaactggtcc atgataccag agttgactcc gataaagttg
    17521 ttttctgttt gttttccgat tttaaccaaa tccaaacggt ctagttttaa atcaaagaga
    17581 tgctctgcca cgactcctgt caagagctcc aaggatgctg aagaagacaa accagcacca
    17641 tttgggatat tcccataaac ataaaaatca aaacctttgt caatcacgtg cccagcttct
    17701 tgcaagaaat gaagaacccc ttttggataa ttggtccagt tgtgctcttt ttcaaacttg
    17761 aggtcagcga gaggcacttc gataatgccc ttgtcctcaa agttagctga gtagaaacgc
    17821 aagacttggt cgtcacgctt acgagctgca ccgtaagttc ccaaggaaat agcagcagga
    17881 aaaacgtgcc caccgttgta gtctgtgtgt tcaccaatca aattaatacg gcctggtgaa
    17941 aagaaggttt ggtctgcttc ttgaccaaaa acagcaagaa agtctttgcg aaggacttca
    18001 gtagtaagat gttgtgccat atgaattctc ctttgactgt ccgttaagcc atcttaacgt
    18061 gtaatcgttt tctttctatt gtatccaagg aatttcccaa ggtcaatcct ttttactaaa
    18121 attttactaa aatatcagta aaaagaagaa aaatatgata tactaaccta atcaaggagg
    18181 atttatggct accttaaaag acattgcaca gctagcctct gtctcaatcg cgaccgtatc
    18241 ccgtgtcctc aaccgcgacc agagcctatc tgttacagaa gaaaccagac accgtatttt
    18301 aaccgttgct gaagagctag gctacaccaa acacctcaag acaggcgagt cccacaagcc
    18361 caagcaaaag attgccatta tccaatgggt cagcgaacaa ggggagctag acgacctcta
    18421 ctactaccag attcgcctag gaatagaaaa aagagcccaa gagttggact acgatatctt
    18481 gcgctatttt aatgaccatc cttttacctt aagcgaggaa gtgattggga ttctctgcat
    18541 cggaaaattt agccgagctc agatttctgc ctttgaagaa taccaaaagc ctcttgtatt
    18601 tctagacagc gatacacttt ccctgggaca tacctgtatt atcacggatt tttacactgc
    18661 tatgaaacag gttgtcgatt atttcctcag tcaagggatg gaccgcatcg ggattctaac
    18721 agggcttgag gaaacaacag accaagaaga aatcattcag gacaagcgtc tagaaaactt
    18781 caaaaactac agtcaagcga ggggaatcta tcatgatgaa ctggtctttc aaggaagatt
    18841 tactgcccag tctggctatg acttaatgaa ggaggccatt cagagcttgg gagaccaact
    18901 tccgccagca tttttcgcag ccagcgatag tttagctatc ggtgctctcc gtgccctcca
    18961 agaagctgga atcagcctgc cagatcgcgt cagcctcatt tcattcaacg acaccagtct
    19021 gaccaaacag gtctatcctc ccctctctag tattacagtt tatactgaag aaatggggcg
    19081 agcaggtatg gatattctta acaaggaagt cctccacggt cgcaaaattc ctagcctgac
    19141 catgctggga accagactga cattaagaga aagtacccta aatcaagaat aggataacat
    19201 aaaaaacgaa tagagttcta aaactcctat tcgtttttta ttcgattaca atcatagact
    19261 taatggtctt acgttcatcc atatctttgt aggcttggtc gatatcttcc agtttataac
    19321 ttgaagtaaa gacgcgacct ggattgatat caccatcaag gacggctttt agtaaaaatt
    19381 gcttatcgta tgttgtagca gaagctgccc cacctgctac agagatattt tgcataaatg
    19441 tcgaaccaag agcacgatta ttatagtgtg ggactcctac aaagcccata cgccctccat
    19501 tatgaagaac acctagcgcc tgttctatag cagcctccgt accaacacat tcaagtgctg
    19561 cgtctgctcc tccaccgagg atttcacgca ccttggtaat tccttcttga ccacgttctg
    19621 caacaacagc tgtcgcacct gactccatag ccatcttttg acggtcttca tgacggctca
    19681 taaggataat ttgtgatgct ccacgcatct tagccgcgat gacagcacat tgaccaacag
    19741 ccccatcacc gataacaaca accttgtccc ctttttgaac atttgcaaca cgcgccgcat
    19801 gatagcctgt cggcatgaca tctgcaagag tcaaaaggga cttgagcatc ccttctgtat
    19861 agtcagaagg ttgaccaggg attttaacca gcgcccagtt tgcatagtgg aagcgaatat
    19921 attctgcctg aaaatcaccc cccaaattat tgccaatatg attgtcgcaa gaaccgtcaa
    19981 atccagcaag acaggcatca cactcaccac atccatgtgt aaaagggaca atcacaaaat
    20041 cacctggttt caccgtcgta atggcttccc cagcttcttc aacaatccca atcgcttcgt
    20101 gtccactatt tttgtgtcca gctttcgttt ctggattacg gtacctccat aaatctgaac
    20161 cacaaacgca cgcacgaacc acacgaataa tcacatcatc cgcttctatt atttgcggac
    20221 gttcaatgct agcaagtcca acctgacctg cctttgtata tactgctgat ttcatttaaa
    20281 attttccttc cttataaagt ttaattttga gatttaaacg atttaaagct gtctgtagct
    20341 gagacaagcg ctcctctaat ttttgctttt cctcttctaa aataccaagc ctctcctctc
    20401 tcgtttcatc tcccttttgg tagagcgaca tatagtcaac taaactatct acagagacac
    20461 ccgccgaacg aaaacactta ataaattcca gcgcttcgat atcctgatct tgaaaatcac
    20521 gaatcccagt agcagtacga gtaatcggtg gcacaagacc aacccgttca taataccgaa
    20581 tcgtatccgc tgaaattccc aacaaatcac tggcagattt aatattcaca cgctaacctc
    20641 ctctcatttc attcactgaa ttcacgctat tctaactcac ttatcttatt atataacttt
    20701 gagttgactc caagtcaagc attttgtatc taatcgaaaa atttttatca ttaacaatca
    20761 tata
//
LOCUS       DAVLXF010000028        20313 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLXF010000028 DAVLXF010000000
VERSION     DAVLXF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20313
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.028"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(237..1844)
                     /locus_tag="VYO12_000968"
     CDS             complement(237..1844)
                     /locus_tag="VYO12_000968"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1587605.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2234..2821)
                     /locus_tag="VYO12_000969"
     CDS             complement(2234..2821)
                     /locus_tag="VYO12_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1587606.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2949..3260)
                     /locus_tag="VYO12_000970"
                     /pseudo
     CDS             complement(2949..3260)
                     /locus_tag="VYO12_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3158..3574)
                     /locus_tag="VYO12_000971"
     CDS             complement(3158..3574)
                     /locus_tag="VYO12_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587607.1"
                     /translation="MKVNIVDLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFFGLKSRRRENGFQLGILHYLRIISPL"
     gene            complement(3602..4252)
                     /locus_tag="VYO12_000972"
     CDS             complement(3602..4252)
                     /locus_tag="VYO12_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1587608.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIR
                     GDLPDYLTILFRSLTHLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASFCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4242..4805)
                     /locus_tag="VYO12_000973"
     CDS             complement(4242..4805)
                     /locus_tag="VYO12_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1587609.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(4970..5204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5258..5761)
                     /locus_tag="VYO12_000974"
     CDS             complement(5258..5761)
                     /locus_tag="VYO12_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1587610.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(5954..6784)
                     /locus_tag="VYO12_000975"
     CDS             complement(5954..6784)
                     /locus_tag="VYO12_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1587611.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6769..8451)
                     /locus_tag="VYO12_000976"
     CDS             complement(6769..8451)
                     /locus_tag="VYO12_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587612.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHCPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNLSIS
                     KGQVQLQNELAKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8562..8810)
                     /locus_tag="VYO12_000977"
     CDS             complement(8562..8810)
                     /locus_tag="VYO12_000977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587613.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVR"
     gene            complement(8817..9365)
                     /locus_tag="VYO12_000978"
     CDS             complement(8817..9365)
                     /locus_tag="VYO12_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1587614.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9380..10228)
                     /locus_tag="VYO12_000979"
     CDS             complement(9380..10228)
                     /locus_tag="VYO12_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1587615.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10416..11765
                     /gene="trkA"
                     /locus_tag="VYO12_000980"
     CDS             10416..11765
                     /gene="trkA"
                     /locus_tag="VYO12_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1587616.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIETKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11769..13208
                     /locus_tag="VYO12_000981"
     CDS             11769..13208
                     /locus_tag="VYO12_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1587617.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13333..15012)
                     /locus_tag="VYO12_000982"
     CDS             complement(13333..15012)
                     /locus_tag="VYO12_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1587618.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            complement(15082..16494)
                     /gene="lacG"
                     /locus_tag="VYO12_000983"
     CDS             complement(15082..16494)
                     /gene="lacG"
                     /locus_tag="VYO12_000983"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1587619.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKYNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(16504..16848)
                     /locus_tag="VYO12_000984"
     CDS             complement(16504..16848)
                     /locus_tag="VYO12_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1587620.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(17016..18902)
                     /locus_tag="VYO12_000985"
     CDS             complement(17016..18902)
                     /locus_tag="VYO12_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEV1587621.1"
                     /translation="MVIRVFDKQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRRDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRNYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWKNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(18904..20226)
                     /locus_tag="VYO12_000986"
     CDS             complement(18904..20226)
                     /locus_tag="VYO12_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1587622.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
BASE COUNT         6262 a         4526 c         3514 g         6011 t
ORIGIN      
        1 gatacaatcc agttcctaaa actttgctag aaattaattt ggctttccga attaatttgt
       61 tcagatttta tttcatttca atatactgat aaatccattc actagcacag ggcaagaggg
      121 agtcgctatt gatttcaacg gtagcaggta catacagagg actcctacct cagatatact
      181 gcaatatcat cgtatgcaaa aaacctctga gatttttctc aaaggttctg attttgctaa
      241 ttgctgttct caaccgctgc agtaacaaag gcagtgtaga gttcttctgg tcggtttgga
      301 cggcttgaca gttcagggtg atactgacaa gctacaaaga atttattttc aggaatttcc
      361 acgatttcta ccaaacgatt gtctggagaa actcctgaaa agacaaaacc tgctgcctca
      421 aactgctcac ggaaggcatt attaaactca taacggtgac ggtgacggcg ttgcaccact
      481 tcttgattgt gataagcagc agcagcctta gagccacgtt tcaacttaga cggataaagt
      541 cccaaacgaa gggttccacc catatcctca atatcaatct gatcacgcat gatatcaatg
      601 atagggtatt ttgtttctgg tgcaagctct gcagaattgg caccttcaag acctaaaacg
      661 tgacgagcaa actcgataca tgtcaactgc attcccaagc agactcccaa cattggaaca
      721 tcattttcac gcgcatagcg gatggcttgg attttccctt ctgtaccacg ttgaccaaaa
      781 ccacctggta cgatgatccc gtccgcatca gacaagagtt ctgctacatt ctctgctgtc
      841 acatcattgg cattgaccca attgatttta acttctgcat cattgacata gccagagtgt
      901 ttcaaggctt cgaccactga gatataggca tcttgcaact ccacatactt accaacaagg
      961 gaaatcttaa cttgtttctt gaggttcatg accttgtcca ccatggctga ccattctgtc
     1021 atagccgctg ctggtgcgtc taatttcaaa tgatcacaaa caatttggtc catcccttgt
     1081 gcctgcaagt tcagtggaat ttggtaaagg tgttcaacat ccaacgattc gataacggct
     1141 tctggtgcca catcacagaa ctgggccagt ttatttttaa ttccttgacc agctggctct
     1201 tctgtacgaa taaccaacat atttggttgg attcccaagc cacgcaattc tttgacagag
     1261 tgttgggttg gtttggtttt catttcacca gcagccttga ggtaaggaag caaggttgta
     1321 tggatataca tgacattatc cgcacccaca tctgccttca tctgacgaag agcctctagg
     1381 aatggcaagg actcgatatc tcctactgtt ccaccaacct ctgtgataat gacatcagag
     1441 tcggtcgtta gagcggcacg cttgattttt tctttcaaag catctgtgat atgaggaatg
     1501 acttgaacag ttgccccaag gtattctcca cggcgttctt tacgaagaac ttcactgtaa
     1561 attttcccag ttgtcacgtt ggaatatttg ttgagattga tatcgatgaa acgttcatag
     1621 tgacccaagt ccaaatctgt ctcagctccg tcatctgtca caaaaacttc cccgtgctgg
     1681 taaggactca tggttcccgg atcaatattg atataagggt caaacttttg aatggttact
     1741 ttgagaccac gatttttcaa gagacggcct agactcgctg ccacaatccc tttcccaata
     1801 gacgatacca caccaccagt tacaaaaata tatttcgtag acatagattc ctctttctaa
     1861 aatgctcaag gtcttgttaa ctacgagggg atatagaaaa caagacccta ctaataagaa
     1921 taatctggga cgactgcacc agattcacaa ctaatactct tcgaaaatct cttcaaaccg
     1981 cgtcaacgtc gccttgccgt agatatgtgt tcctgacttt gtcagtctta tctacaacct
     2041 caaaacagtg ttttgagcag cctacggcta gtttcctagc ttgctctttg attttcattg
     2101 agtataaaat acaagaagat aacttcttga aaaaacaaaa atagctccct aagaactagg
     2161 gagccccgac ctctaaaaga ggtgcccaaa caatatgata cctaaaaata ggataattgt
     2221 caatagctaa ctcttattcc tcgctggttt cagcgtcgtc atctgaaaac tcgtcgtcgt
     2281 cttcgttgag atccacatct tctcccaagt catctggagc gatttcgttg atttctgcat
     2341 cataagcttc cacttcattt ttttcatcat ctggattttc atcatcgtat gaaagagctg
     2401 gatctgcttc gtatgcatct tcgtcttctg gatcatctgc attgtagtca atggcatctg
     2461 aatcaccatc cataaaggca ttgacacgtt ttttcttagc ttttggtgct acttcatcgt
     2521 cgtcattttc ttcaagagcg atgatttctt cgtcgatttc gtccacacca taccatgaac
     2581 gaagacccca tttgttgtcc ccaagtgaga tgaagctacc gtcaaagttc aactctgtgt
     2641 agaacagagg caaagcttcg cggatatcgc tgtttgatgt tccaaggtag ttttgaattt
     2701 cgtttacaag atcgctaaaa tgcatctcat gatcgcgacc acgaagttct aatatagcac
     2761 gcgctacctc aatcatagat agttcacttt tttcttgccc agcaaatact tctaattcca
     2821 aagcgtttct cctcatttat actactatcg ccagagcgaa cagactctga cctcatttta
     2881 tcatttactc tttattttac gataattttg cggaatagtc aaaggttaag ggggagaaag
     2941 tggcaggatt agactaattc caatataaaa ctccttcctt tttctgttgc tccattttcc
     3001 acaaatccaa gcgacttgaa acacctccta gaagcatgat tgtaggtgta gatttccttg
     3061 actctcaatt ctttccatcc ttttactcga gccaattcaa tcaaagcact tagaatcttt
     3121 tttccaagtc ctcgatgttg gtaagcggaa ttcccaatca caatggggag attatcctga
     3181 gatagtgtaa tatccccaat tggaaaccat tctcccttct ccttgacttc aatccaaaaa
     3241 agctcaccat gccgatccaa agaggtaaga tatgattttt taaatcaaaa acagagtaaa
     3301 tctttctttc ctcgcaagct tgcgcataga gatgatatag ttcatcacca ccatctctat
     3361 cccactcagc agaaatcgta tcccgacctg ccaataaagc ctgataagcc ctgtgatgcc
     3421 catctgtaat cagcaaacaa tctccaaagg caagaatact gattggatcg acttggtttg
     3481 tttctgccga ctggtaaagc atctgaatat cttgcaactt cttttctgat aaatatagtt
     3541 gagtcggatg aagatctact atattgactt tcatttcttt ctcctaaagg gaattcgata
     3601 ctcacttctg tttgccttta aatcgccatt ggaagcggag cttgtcatag aagggaaact
     3661 cgataaacaa gactcccaaa cccacacaga aactggcaag aacatctgat gggtagtgaa
     3721 ctcccagata gactcttgat accagcacac tgactaggta gaggccaagg acgatttgta
     3781 cgatttttct ccagacctga tctttaatcc gctgactaag aataacaatc aaagtcccta
     3841 ccatcagcgt tacagctaga gaatgcccac ttgggaagga aaatcccttc tcctccacca
     3901 gatgtaagat agctggtcgt gggcgctggt agatattttt aaaggtcacg attaaaagac
     3961 ctgccaaagc cagatttccc agcatgaaga aactttctat cttccatcgc ttacgataaa
     4021 agacaaaagc tataatgaca acccaagtga taatcactgg aatatcaatc agatgtgtga
     4081 gggatcgaaa aagaatagtc aaataatctg gtaagtctcc tcgaatggca gtctgaatcg
     4141 attggtcaaa accgaccagc gtttcagggt aaaatttgac catatagcca agaataacga
     4201 aaagtaaaag ggcaaaactg cccttcatta aaaatgtttg tttatctttc atagtgtttt
     4261 aaggttggtt tcaaaaggac gtacaacaac cagaatgaaa cggaaaagat tacaccctca
     4321 atcaagttaa aaggtaatac catggtcatt aggtagttgg aaagtcccaa aatttttcca
     4381 atatcaaagt tagcaaactt agcgtacaaa ggaacagcat aaacatagtt gagaaccaac
     4441 atggccaagg ttaaaccaac agttccagct agagagccta gtaggaaacg aagggttgtc
     4501 cgttcctttt tccaaatcaa agcaaatacg atgacaaaaa ctcccaaagc tacgatattc
     4561 atcggcaaac caatgtaagt attcactcct tggctgttaa gaagcaattt caatagtgag
     4621 cgaagcaaga gcactcctag agcagcaggc aaatccatga ccaccagacc cacaaggact
     4681 ggcaagatac taaattcgat cttgaggaaa gatgccgctg gtaaaagcgg aaagtcaaag
     4741 tacatcagca caaatgagat ggctgataga attgcaatgg tcgaaagtcg acgtgtgttt
     4801 gtcataacag gttcctccaa ttttctataa aatcagaaga agttggaaag gattcctcta
     4861 tctattctca ctttttatat cccaaaagtt ccctcttact ctattaaaga aaaacaaagc
     4921 aagtggttac aatccggcta taaatctatc aaaacagaca aagctattct ttcgtcttct
     4981 cccatccaga ctatactgtc ggttgtggaa tctcaccaca tcagcttgcg ctcgcggact
     5041 tctttaaaga gaaggaaata gagatttttc ttttagacta ggcgacaagc tgtaggcata
     5101 acttctggtt aggcaaagct tgtcaacaaa gtataaaagg aaaagatcat ttcctagtta
     5161 aagtcaccgc cggtcgggaa tcacaccctg ccctgaagac tcttttatca taacaaaaaa
     5221 cgcttgcaag cgcaagcatt ttgttcattt tcatttttta tttcaattta tccacttcat
     5281 aggtatggac gagctcaaga cctgcaaagt tctgctggcg aagagcctca tagacaatca
     5341 tgcagacggt attagacaca ttgagactgc ggacatgttc atcattcatg ggaatacgga
     5401 gagccttctc aggatgttct cgcataaaat cttcaggtaa gcccttgtct tcacgtccaa
     5461 agagaaaata atggtcctcg tcagtcgata aatccacctc agaatagact ttttccgcga
     5521 atttcgaaat cagatagagt tttcccttca tctgagatat gaaatcttcc aaactctcgt
     5581 aaaaataaat ctctagctta tcccagtaat ccaatccagc ccgcttcatc ttgcggtcat
     5641 caataggaaa gcccataggc ttgatgatgt ggaggggaga attggtcgca gcgcaagtac
     5701 gcgcgatatt gcccgtattt tgtggaattt gaggttcaaa taatacaatg tgatttgtca
     5761 tgacttgctt cctttcacca ttgcaaaaaa atagccacac tgcccggagt caagctcagc
     5821 aaacagcgtg gttaaggcat cgttaactta cctcacaaca ggtttgaagt aaatcagcga
     5881 aactactttc ttagtataac actttcagaa tcattgtcaa tagaaatgac ttgatttttt
     5941 caattttttc aagctatttc caagggttgt aaaatcgtcc ctgattctgc aagataagta
     6001 gtaaactaac tactaaaaac aaggttgcca agagcaaggt aatatagtct ccttttttca
     6061 aggcctgata actataccat gtgcgttttt tctctttccc aaagcggcga agctccatgg
     6121 cagtcgcaat ggtatcaatg cgttctagcg agctaaaaat caagggcgta ataatgagca
     6181 gattgccttt gattcgttgc ataagagaag ctttcttgga taattccatc ccacgcgcct
     6241 cctgagacat cttgatagta aagaattctt cctgcaaatc tggaatatag cgcaaggtca
     6301 ggctgacaga ataagcaatc ttatagggca caccaatttg atttaaactg gaagcaaact
     6361 gactaggatg ggttgtcatc aaaaagataa tagccagagg aatggtacaa agatacttaa
     6421 tggccaaatt tagcagataa aagagctcct ggctggttag agtgtagaca ccgattccct
     6481 gccaaatcac acttctctct ccataaagtc caaccccata ctcgggagaa aagagataga
     6541 ccatcaaaac gtttaaaacg gcaaataccg tcgcaaaaac ggctacaaag gaaacatctt
     6601 taaagcgaat ttctgataaa tagaggagaa agactgaaaa gatggcaatc agcaagagca
     6661 ttctggtatc atagctaatc atggccgcca atgataccag aatgaaaaag agaagtttcc
     6721 cagctcctga caagcgatga atcacagtat ctctatgctg gtaaccgatt aatttagctt
     6781 gcatccctct ctcctttctt tgtaaaatgc cgttaaatcc agtggatcca catctagttt
     6841 cttagccaag ttaaagatgg aggtttcttt tagattggct tttactaaca gctcaggatc
     6901 gctcaacaga ctggctggaa cagtatcggc aatcaattct ccatccacca tgacaaggac
     6961 ccggtctgaa taatccagca tcaattgcat atcatgggta atcatgacaa tggtatgccc
     7021 tttttgatgt aactcttcga gaaattccat aatctcagta tagttcttct gatcttgacc
     7081 tgcagtcggt tcatctagga gaataatttc agctcctaag accaaaattg aagcaatggt
     7141 gacacgtttt ttctgaccaa atgacagggc agaaataggc caattacgga attcataaag
     7201 tccacagatt ttcaaggttt catatactct cgtttcaatt tccttctcat ccacacctcg
     7261 caaacggagc cctagagcca cctcatcaaa aatcatattg gttgaaatca tttgattagg
     7321 attttgtagc acatatccta ctcgttccgc ccgctctgca acagaatcgc cttttatatc
     7381 ctgtttttcc caaagatagc gtccttccgt ctgaataaag ctacttatag ccttggctag
     7441 agttgatttc cctgctccat tttttccgac aatagcaatc ttttcaccct ttttaatatc
     7501 taaatgtagg gattttaaaa tcggtctatc atcataagaa aaagatactt cctctagtct
     7561 aaagagtgac tgcaatgctg gggtttcttt tgccagttca ttctgcaact gaacctgacc
     7621 ttttgagata gacaagttat ccagattcgc taattgttct tccttgacta aatccacacc
     7681 caattgacgg agagtggtga gataaagggg ttcgcgaatt ccattttggg tcaacaaatc
     7741 agtcgccagt aactggtcag ggctcccatt aaaaaggata cgaccatcgt ttatcaagac
     7801 aatccgatcc acagggcaat gcagaacatc ctccaaacgg tgctcgataa taagggtcgt
     7861 cgtcccctcc tccttatgaa tctggtcaat caattcgata atatcctgac ctgacttggg
     7921 atcaagattg gcgagtggct catcaaacaa gagaatcgga ctttcatcaa tcaagacacc
     7981 agccagactg actcgctgct tttgtccacc tgacaaatcc tgagggcgct gatccagtaa
     8041 aggaagaagg tccagctttt cagcccattt ataaacacga cctttcatct catctagggc
     8101 tgtcacatca ttttccagag caaacgccaa atcttctgcc acagacaagc caataaactg
     8161 cccatctgta tcctgcaaaa ctgtgctaac cagatgagac ttatcataga tgctcatatc
     8221 aaaggctgtt tgacccttta tcaaaaattc tccatatgtc tgacccttgt aaatattggg
     8281 aataatccca ttcaaacact gacccaaggt agatttacct gatccagatg gtccaacaat
     8341 taagactttc tctcccttgt aaatggtcaa gtctatccct tgcaaggtcg gttcttgttg
     8401 tgtttcatac cggaaagaga aatccttcca ctcaattata gcttctttca tcttactctc
     8461 ttcattcgct tcttagactt ctattttatc ataaatcaag cccttcttgc agtctctcct
     8521 attaaaatct tagcgccaaa aagattccta tcctagctta cttacctaac taatctataa
     8581 acatcgaaaa agactagttg cccagccttc cccatcattt tatactcttc gaaaatctct
     8641 tcaaaccacg tcagcttcgc cttgccgtag gtatggttac tgacttcgtc agtttcatct
     8701 acaacctcaa aaccatgttt tgagctgact tcgtcagttc tatccacaat ttcaaaacac
     8761 tgttttgagc aacctgcggc taacttccta gtttgctctt tgattttcat tgagtattag
     8821 tcctttttca aacttcctgc acgagtttgg gttcctgcat aggcaagtaa gagaagagtt
     8881 cctgcaatag ctacagatac accattggca attcccgcaa caatcccttg tgcaaatact
     8941 ttttctgccg cttcttgata aatcacaaca tctccaagtg gtgccaagac accccaaaca
     9001 agggcatttg caagtagttg aatgaggtta aaaataagaa tatctttcca gtcaaaaaca
     9061 ccattgatca cgcgaacgta ctttctaaaa agtcccacaa ctaaaccaaa gagtccgcta
     9121 gcgataatcc aagtccacca tagaccataa ccagcaagag agtccttgat tgcatgacca
     9181 atcaacccga caagcaaacc gataatcggt ccaaaaataa tagaaagtag cgcttgtacc
     9241 gcatactgaa gctggatgct tgtatttgga acaggggttg gaatgttgat catcccgatg
     9301 acgacaaaga gggcagcgcc aattccgaca gcaacaactt gtttaattgt aaatttaatt
     9361 tccatactat tctcctattt tatccttcta ttttctttat ttcaatggtc caagatgaac
     9421 cgacacctac attataggcc ttggcaaagg aaccttggtt gatagccaaa cctaaacgat
     9481 agagagagtt gatgtaaagg atgggttgcc caattctcac atctgcaaat gatttgccat
     9541 agacaacctg attttgatag accagcatat cagcatgata gatggtcact tcaaaacgat
     9601 caccaaattc tggttccagc ttgtaaaatt cttcccgtgt gatagaggtc caaagcgaac
     9661 cgaaacgcac atccagaata tcaatggctc ccttcaccag atgatcttct atgatggtcg
     9721 ctacgactgg aagctctaca atctgttcca cactgagctc tggccctact tcctcaaaag
     9781 taatgtgacc actggccagt ttagcaccag tataggcata gacatcacga ccgtggaagg
     9841 tataagaatg ctctgtgttt tgacgcctat tggccacctc agaaatctca cgaatggcta
     9901 caatgccaac gtgtttcttg ataaaggaaa gcgtcccatt atctggcgtg acaatgtatt
     9961 gattttttgc agtcttggca actacactct tacgtttcga accgacacct ggatcgacaa
    10021 ccgatacaaa cgtcgttccc tcaggccagt aatccaccgt ctgaaagaga cgatagctcc
    10081 cctcaaaaat attataaggc gtgatatcgt gcgtcaagtg atggattttt aaggttggag
    10141 actcttctaa agccactcca atcatagccg ataccgcacc atcaaccaga ccaaagtctg
    10201 attgtaatac cagtaaatta ttattcattt tatctccttg tatatccttt atcataccat
    10261 atttcagaga aagtcgctaa tagctatttc aattggttag ggtatagttt taccttatag
    10321 cgaattttca ctaaaagctc ttgttattag ctagtaggtg cattttttct tgaaaaaggg
    10381 tatgatagtt acatcatgaa aaagtaggtt ttattatgaa aattatcctt gtcggagggg
    10441 gaaaagttgg ttttgccctc tgtcgctcct tggttgcaga aaagcatgat gttttgctga
    10501 ttgagcaaga cgaagctgtt ctcaatcata ttgtcagtcg ctttgatatc attggtatcc
    10561 ttggtaacgg ggccgatttt gccattcttg agcaagccag cgtccaagat tgtgatatct
    10621 ttatcgccct gactgagcac gatgaagtca acatgattgc agcagttcta gccaagaaaa
    10681 tgggagctaa agaaactatc gttcgggtgc ggaaccctga atattctaac tcttatttca
    10741 aggaaaagaa tattctcggt ttttctctta tcgttaatcc tgagctcttg gctgcccgcg
    10801 ctatcgcgaa tatcattgac ttccccaacg ccctgtctgt cgaacgcttt gctggtggac
    10861 gcgttagcct catggaattt gtcgtcaagt ccaccagcgg tctttgccaa atgcccattt
    10921 ctgattttcg taaaaaattt ggtaatgtca ttgtctgtgc gatagagagg gatcatcaaa
    10981 ttatcattcc aagcggtgac atgactgtac aggataaaga tagaatcttt gtcactggta
    11041 accgtgtcga tatgatactc ttccataatt attttaaatc acgcgccgtg aagagccttc
    11101 tcatcgttgg agctggtaga attgcctatt atctattagg tatcctcaaa gatagtcgta
    11161 tcgaaaccaa ggttattgaa atcaatcctg aaatcgctag cttctttagc gagaaattcc
    11221 caaatctcta catcgttcaa ggagatggaa ccgcaaaaga tatcctgctg gaagaaagtg
    11281 ctcaacacta tgatgccgtt gcgactctaa caggagtcga tgaggaaaat ctgattacat
    11341 ctatgttcct tgacagggta ggtgtacaga aaaatattac taaggtcaat cgtaccagtc
    11401 tcctcgagat tatcaatgcg cctgattttt caagtatcat cacacctaaa agcatcgctg
    11461 tagatacgat tatgcacttt attcgtggtc gagttaatgc ccagtattca gaccttcaag
    11521 ccatgcacca tctagccaat ggccaaatcg aaaccctgca attccatatc aaggaagcca
    11581 ataaaatgac tgccaaacct ctttctcaac tgaaattgaa aaaaggggtt cttattgcag
    11641 ccattattcg aaagggcaag actattttcc caactgggga ggatatgttg gaagttggag
    11701 acaagctcct agtaacaacc ttgttgccaa acatcaccaa gatttatgac ttgattgcga
    11761 ggtaagaaat gaataaaagt atgattcgtt acctcctttc aaagttactt ttgattgaag
    11821 ctgttctcct cttggttcct gtggctatcg ctgtctatta ccgtgaatcg agccaagtct
    11881 ttacagccct cttttcgacc atagggattc tcgtattgtt aggcggttca gggagtttac
    11941 agaagccaaa aaatcaacgg atttatgcca aggagggagt cttgatcgtt gccctctgtt
    12001 ggatcctttg gtctttcttt ggtggtctcc cctttgtctt ttctgggcaa atccctagcg
    12061 ttattgatgc cttttttgaa attagttctg ggtttacaac tactggagca agtattttga
    12121 acgacgtttc ggttctcagc cgttccctcc tcttctggcg aagttttacc cacttgattg
    12181 gagggatggg agtgcttgtt tttgcacttg ctattatgga caatgccaaa aatagccacc
    12241 tagaggtgat gaaggctgag gttccaggtc ctgtttttgg caaggttgta tccaaactaa
    12301 aaaacactgc ccagattctc tatctccttt atctagctct cttctccctc tttgtcatca
    12361 tctattatct agctggtatg cctctctacg atagttttgt cattgctatg gggacagcgg
    12421 gaactggagg ctttaccgtc tataacgacg gaattgccca ctatggcagc tcactgatta
    12481 cctatctggt cagtatcgga gttctggttt ttggggtaaa tttcaacctc tactactacc
    12541 tcatgctccg tcgcatcaag gccttctttg gtgacgaaga acttcgggct tacttggtca
    12601 ttgtactggt ttctacaggc ttgattagcc tcaacaccct ctacctctac ccagggtttt
    12661 caaagagctt tgaaatgacc ttcttccagg tttccaacat cattacaaca actggttttg
    12721 gatatggaga tattaccaac tggcccctct tctcccagtt tatcctcctt ttcctcatgg
    12781 caatcggtgg ctctgctgga tcaactgcag gtggactcaa gattattcga ggcctcatcc
    12841 tttcaaaaat tgccaaaaac caaattttat caatcctatc gccccaccgt gttttgactc
    12901 tccatgtgaa tcaaacggtg attgataaag atacccagca taagattctc aagtactttg
    12961 tcatctatgc tatgattttg ctagcgctta tctttattgt cagcctagat agcaatgatt
    13021 ttctggtcgt gaccagcgct gtctttagct gtttcaataa tatcgggcct attctaggaa
    13081 ccacttctag tttctcaatc tttagtccca tctcaaaaat tctcctctcc tttgcaatga
    13141 ttgcagggcg cttggagatt tacccaatcc tccttctctt tatgaaaaga acttggtcta
    13201 agagataaaa tacaaaatcc ctctttttac aattgtgaaa gagggatttt tatattaaaa
    13261 agggggaacg tttttaaatc caagcctgtc tgtaagcaaa gttcttactc aaataaaaga
    13321 tggaaaaact tactacacag cttggtaatg ctccaataca aattgtaagg ctttacttgg
    13381 attctttgtt aaatgaatat attccattcc tctggtagca actatctgaa tacctaatct
    13441 ttctgcatcc accttcatct ctctataata actccgaact tgtggagcca gaataattaa
    13501 atcataaaca cccataatat cataatgagc tccgtacgct cctgaattcg caatcactct
    13561 aacttctgct aattgagcgc cctcgttaat tgcattggct aattgcgcac ttgttccaga
    13621 ccctgcacaa agaaccagta ctttcaactc cttacttttt atttcatcta actcaccagg
    13681 cattatttca cttgtctcct cctctaaaat aatatcattt gagcttgcaa catcttcttt
    13741 catcagtaac tgtctatcat acgctctaca gaatggcaaa taaatcaata tgtcgacaac
    13801 taaaatcaaa gataaaaata caaaggagat agattgaaaa ttcgttccaa ttaacaatcc
    13861 caagggacca ggaaaggccc aaggtaactg gatataaaat ccattcattc caaagaaatc
    13921 aataaagacc tttcctagaa atacattcac tggtggagtc atcaaaaaag gtacaaaaag
    13981 atagggattc aaaataactg gcataccaaa tagaagaggt tcattcactg caaataaaac
    14041 aggagtaatt gtagctttac ctaccgattt taattgttta gaccgcataa agaaaatcaa
    14101 aataaatggt acaacaaagg tagcccccgt tcctccgata gcagcgatat aatttccgaa
    14161 attaggcgtt aaagaatgat aaggaaactg cccatttgca agaagttgag cattctcttc
    14221 catattgctc agttgtaaag ctgtaacagc aggtaagaca atagatggtc catgaattcc
    14281 aacaaaccaa agcaaagcgc acataaacca aatcaacatc atagcaggat atgattctgc
    14341 ccccttaaaa ataggagtca atagttgttg aaatacttgg gcaaaaggaa catctaaact
    14401 aaaccgagat acaatatcta acaaaccact aataagtaaa acaaaagaaa aaggaaaaat
    14461 atctctaaaa gcttgtgata tagccccagg aacttcctta ggtaaatgaa tagtaatgtc
    14521 tcgcttaata cagaatcggt aaacatttac agtaataaag gcactgacaa acgaagttat
    14581 caatccttga gatcctaaat agtttgtcga caaacttgta gatcccgttt tctcatcaac
    14641 tacaagcgtt acagttaata caagatagcc acatatggcc gccaacattg cagaagtatc
    14701 atttattacc tttccaggag gcatttttct attaacattt ccagccaatg actttgcaac
    14761 tgtccctgaa acaataacac ccaaaactcc catggttagc atatacactt tcctcatcca
    14821 ggcaatagct ggactcggta actctactcc gaaattttta ggaatcataa taataatcat
    14881 caaaaagctt gaaaacataa ttaaaggcat agtagcaatg aatccatctt taatagccat
    14941 catataatta ttagtagaaa ttttttcaaa tttcccttgt tgcttttcaa tggcagcgac
    15001 taacttatcc ataaaaactc ctcctgtaaa aaatttaagc tcatagttaa aatatattaa
    15061 acatcagtca aatcgctaac cttaattccg ttttagccct tttacccaaa gagcactttt
    15121 cttaatatat ctcttctgtg tttcataatc aacaaagaaa agaccatatc gcttattgta
    15181 gccattcgcc caagaaaatt gatcttgcaa agaccatata aaatagcctt gaatattgac
    15241 tcccctatct cgagcctcta aaactttgtg taaatgttgc tcaatataac ggattctttt
    15301 actgtcatca ataatatcat tctcgccctc tggcttaacc tctttaaggg ctgtaccatt
    15361 ttcagttaaa taaatgaccg gatgttgagg atattcttct ttgatacgaa gcaacatatc
    15421 aaataaccct tgaggataaa tattccaatc ccaatctgtt gtcggaatac caggcttttt
    15481 tacaaattca cctagagcat ttaatttaaa agaagactgt ccttttattc ctgttgaatt
    15541 aaattcttgt ctattttcac ctttgtattc acgaataaat tctgaacgat aataattcat
    15601 cccaaacatc gtattaagag gtgcagctct tttcataatt tctaaatcac catcttcaat
    15661 aataaagcta gaattattag cttccaatat ttgattcaag tgaaaaagcg tgtcatcact
    15721 gtagtagccc aaaaaagttc catctaatag aaatttatta ttataaacat cataccgttt
    15781 agctgccaaa atattttctt tttgcccatc aataggatag cctggcttta aagcatgaat
    15841 acaacctacc tttccctcaa tccctaattg atgaaattcg aggactgcaa gagcatgcgc
    15901 caacaacatg ttatgatttg cttgaattgc ttcagataat tgaaaatggt gattcggagg
    15961 aaactgacct cctatgtatt ggcctgcagc aagagacatc agttcattga ttgtaaacca
    16021 atgcttgact tctgtaaatt cttggaaaca aaagcgagca tatcgtatga aacgatcaat
    16081 attctctcta ttcaaccaat cccctgtttc taacattttc tgaggcgaat caaaatgatg
    16141 taaagaaaca aacggaatca cattatattt taagcaggac tgaaagactc tatggtaata
    16201 acgaacagcc aatatattcg gctccccatc tatatcagga aatatacgaa cccaagaaat
    16261 agataaacgc aaagcctgca aaccatgttc tgccgccaaa gctatatcct cttcgtaacg
    16321 ataataaaaa tcactagctg gatctggtaa gaacggacta ttttcttgca aataaacatc
    16381 ccacatattt attcctttgc catctaccct agttgcaccc tctacttgat aagcagcggt
    16441 agcagctcct aataaaaact ccttcggtaa ggctttaacc tgtaaatttt ccatgactaa
    16501 tcctcattgc aaaccaatat gttcttcaat ctttcgaatt cgttcatatt catcaataaa
    16561 aaactttacc tgatcataca atagcatcgt tgtcatcaaa gtatcttgac catgaatcat
    16621 gataaaactc acttcaaaat tatctccata tgcctcctcc gctaataaat tagtctgttc
    16681 tcgatgggca gacactattg agtcgtttgc agactcaacc aattctcttg cctgttcaaa
    16741 atatccatct ctcgcaaacg ccaaagcatc catcaaatct gaccttgcat ctcctgcgta
    16801 agcgacaatt gtaaaaccaa gcatatttat ttcttctttt gatttcatct ttcttcctcc
    16861 tcaatcttga accatctttt acacatcatt attaaaatga cttcgctacg atacaaatag
    16921 aaagtcgacc ctaaatatct taaaaatcct taaatttcat aatatttagg atcaatcttc
    16981 tctgaatacc ttggttcatt ttctagactt atgatttata agtggaaacc agcgtaattt
    17041 tttgctactt caattacacg ataaacatcc aaattagatt ggaatagcag ttcgtgccca
    17101 cggatataat tcaacacatt tttccactca tcttgagctt gccttgtttt tccggttgct
    17161 tgaagatata aagccttcgc catcttgaca acatattcac gatgataact gagctgtttc
    17221 cattcatcct tttgactatt taataactta gaaatatttt cctcaataat tggtaaaaaa
    17281 ttatcagcta gattgtaagc tatataataa tgattcgcta aaacatcact ttgacggctg
    17341 ccaattgcat taaaataatc acaagaggaa taaatggata atttttctaa atattcaaca
    17401 acagactgcc aattttcccc atacaaagca gaaaagtatt cttcaatcaa ttcttcataa
    17461 tttcttgtct tcttccagag catttgcccc atgacataat taggaaaatt atgagggaat
    17521 ccggcacgta attcttgaca cgaaatgtac ccgttcaaat gtaggttgga aagataagat
    17581 acatctctgt aaatagtttg actaattttc atatagccta aatcgccata atgagcacgc
    17641 cctaaaggat agtcatatac gaaactatct cctttaaatg ctttttgcca ctcaaaaaga
    17701 taagataaat tttcctcaag agaattcgga agtataattt tattacgcag ataaggttta
    17761 ggcgtaggta tggaattgtc aaaatctaca tctgcataac tcatttcaaa tgttcttgta
    17821 atcggtgcaa acatcatggt aaagcgttca ggattatcta atttttcttt ctgaggtgcc
    17881 cataacaact catgataaag cagaaaacaa atctttgtat ctaatccctc actcgttaaa
    17941 gccctatcca attgattgag aatacgaata tactgatccg aaaccaattc ttgtctacag
    18001 ttttcgcatt cacaaatatt attacgagca tccgacaacc atacatgtaa gtagttaaca
    18061 tcaggtcttt tcttggcata atccttgata atttctacca tcttatcagc tacatctgga
    18121 tttgaaaaat ccaggctggt taaaatcgga gccgtattaa acaattctcg tttcccgttt
    18181 atttcagcga catagggttt cttctcctct gaaatactaa gacctgattc ccagccaaat
    18241 tttgaagagt aacctaaaac ttcacctgtc catccatgac caacacgatg atgaataaga
    18301 cctctttttt gcaattcttt atccaaccta tcactcaatt cttgtactaa ttcatttgaa
    18361 aattgttctt tatttagata tggattaaat tcatgttcat accaacgttt caaaaaagag
    18421 tagggatttt caaactggat gaaaaaactg ttcatcccaa tcttaggtag ccaatcaatg
    18481 aaatctagta tattttcaaa tgaatccgct ccctctatac atactcccct atgatagtaa
    18541 ctggctgttt catctataga tagctgttta tctaaaaaat cttcaaatcg taactctgga
    18601 acaaagtcat gtccgcgccc tggtctagta tacacaaccc caaattcatg aaacattcgg
    18661 taaattccaa taagtaaacc tacaattgta ttagactcaa ttctcccctt acccttgtct
    18721 aatgagataa gaacatggtc tcttctatcc tctttattga ctaatcctac aaaaatatcc
    18781 gcttccttct cctcgacaag ttctatgtca gtcttaaaga cccgatttat atagtaactt
    18841 aattcctcta aggcaaagct agaataagta tttttctgct tatcaaatac tctgataacc
    18901 atcttatttc cctccaacag tagctttttc tttttcaaga ttgcgtttat ctgcaatttt
    18961 gaagaatgga taataaattg ctacagaaac caaaatcaaa acaacttgta atagagctcc
    19021 tcgccaacta gctcctgtcg caaggaagcc tcctatgatc ggtggcattg tccacggaag
    19081 gattacacct gttgtatagg gtactaatcc tacagccatt gatacatagg taattaaggc
    19141 attgattgta ggagtagcaa taaacggaat cagcataatc ggatttaaaa ctgttggaaa
    19201 cgtaaataga atagctgtat tgatattaaa aatagacggt ataatagcta gcttacctaa
    19261 tgttttattc gcacgactct tactaaatag gaagagacaa atcgcaagac caatagtcgc
    19321 tccaccgcca ccaataaata caaataaatc tttaaacggt aatgtaataa tgtgttggag
    19381 tgtttgtcct gcagccacag cttcttggtt ttctgtagta aattgtaacc aaactgggtc
    19441 tacaaaagca tttaaaactt gtcccccatt aactccacag aaccaaaaga atgagtttac
    19501 aataacacat agaatcatac ctggaagcgt tcctgcaatt aacttaagcg gtgttccaac
    19561 aattgcacct aggagtccgt tgagacctcc tgcaactcct gccgcttcaa gaccttttaa
    19621 gaccagagcc cacaaaacaa aagtagtaaa gccaggtaaa agagctgaaa atgatttact
    19681 taccacatct gggacaccac ttggcatttt tatcgtaatt ccgcgctgga taaacatacg
    19741 ataaatctct gctgtaacca atccaatagt aatcgccata aacaaagaag atgcattcag
    19801 actagaaaat ggtattgcgc cgccaaataa ctgcttgacc tgctccccat ttttatcata
    19861 aaccatactc gaaaaacgag gtgccatcaa tacaaaactg gataaggcta tgatccctgc
    19921 cgacggacca tctgtaccat atccttccga caacctgtag gcaataccga aacaagcgac
    19981 caaggagata atcgctactg ttgactgatt catagttgtc aagatactta gcaatccaac
    20041 actattcagc caatttgtaa aagcctcttg aggccaactg attagaataa gaaataaaga
    20101 gccgactaaa atcaaaggca ttgccatcat aaatccttca cgaatagctt tcaaatgccg
    20161 ttggttgcca actttttcac caaacggagc gacttttgtt tctaaaaacg ctaaaatatt
    20221 gttcatcacc ggaactcctt tttttatttt ttataaatta aatataccac taacagaaag
    20281 cgttgtcaat atattttttg ttatttttac aat
//
LOCUS       DAVLXF010000029        20275 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLXF010000029 DAVLXF010000000
VERSION     DAVLXF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.029"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(96..245)
                     /locus_tag="VYO12_000987"
                     /pseudo
     CDS             complement(96..245)
                     /locus_tag="VYO12_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(399..2030)
                     /gene="rlmD"
                     /locus_tag="VYO12_000988"
     CDS             complement(399..2030)
                     /gene="rlmD"
                     /locus_tag="VYO12_000988"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1587623.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2104..3078)
                     /locus_tag="VYO12_000989"
     CDS             complement(2104..3078)
                     /locus_tag="VYO12_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1587624.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSAHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3152..3766)
                     /gene="pmp23"
                     /locus_tag="VYO12_000990"
     CDS             complement(3152..3766)
                     /gene="pmp23"
                     /locus_tag="VYO12_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1587625.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3766..4740)
                     /locus_tag="VYO12_000991"
     CDS             complement(3766..4740)
                     /locus_tag="VYO12_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1587626.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4748..6004)
                     /locus_tag="VYO12_000992"
     CDS             complement(4748..6004)
                     /locus_tag="VYO12_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1587627.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6066..6497)
                     /locus_tag="VYO12_000993"
     CDS             complement(6066..6497)
                     /locus_tag="VYO12_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1587628.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            complement(6499..7101)
                     /locus_tag="VYO12_000994"
     CDS             complement(6499..7101)
                     /locus_tag="VYO12_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1587629.1"
                     /translation="MMDRIRQELEKGGAVVLPTETVYGLFSKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLSEISFEEA"
     gene            complement(7085..7924)
                     /gene="prmC"
                     /locus_tag="VYO12_000995"
     CDS             complement(7085..7924)
                     /gene="prmC"
                     /locus_tag="VYO12_000995"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1587630.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7924..9003)
                     /gene="prfA"
                     /locus_tag="VYO12_000996"
     CDS             complement(7924..9003)
                     /gene="prfA"
                     /locus_tag="VYO12_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1587631.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9000..9590)
                     /locus_tag="VYO12_000997"
     CDS             complement(9000..9590)
                     /locus_tag="VYO12_000997"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1587632.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9714..9896
                     /locus_tag="VYO12_000998"
     CDS             9714..9896
                     /locus_tag="VYO12_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1587633.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10033..11406
                     /gene="mnmE"
                     /locus_tag="VYO12_000999"
     CDS             10033..11406
                     /gene="mnmE"
                     /locus_tag="VYO12_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1587634.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11453..12388)
                     /gene="dapA"
                     /locus_tag="VYO12_001000"
     CDS             complement(11453..12388)
                     /gene="dapA"
                     /locus_tag="VYO12_001000"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1587635.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12444..13520)
                     /locus_tag="VYO12_001001"
     CDS             complement(12444..13520)
                     /locus_tag="VYO12_001001"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1587636.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDMDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(13862..13934)
                     /locus_tag="VYO12_001002"
     tRNA            complement(13862..13934)
                     /locus_tag="VYO12_001002"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13899..13901),aa:Thr,seq:ggt)
     gene            complement(14034..14543)
                     /locus_tag="VYO12_001003"
     CDS             complement(14034..14543)
                     /locus_tag="VYO12_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1587637.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14541..14637)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14666..15097
                     /locus_tag="VYO12_001004"
     CDS             14666..15097
                     /locus_tag="VYO12_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1587638.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15179..15556
                     /gene="mscL"
                     /locus_tag="VYO12_001005"
     CDS             15179..15556
                     /gene="mscL"
                     /locus_tag="VYO12_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1587639.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15598..16692)
                     /gene="hemH"
                     /locus_tag="VYO12_001006"
     CDS             complement(15598..16692)
                     /gene="hemH"
                     /locus_tag="VYO12_001006"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1587640.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGMRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENIKMPYLIKKMLEKAGKLPKE"
     gene            16846..18069
                     /gene="pepT"
                     /locus_tag="VYO12_001007"
     CDS             16846..18069
                     /gene="pepT"
                     /locus_tag="VYO12_001007"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1587641.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18105..18437)
                     /locus_tag="VYO12_001008"
     CDS             complement(18105..18437)
                     /locus_tag="VYO12_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587642.1"
                     /translation="MDALLDQLSANSKDKTALLESTKAILVLNHQIQSKASASEETSP
                     ARNAEANGDNTSAENQPNATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTT
                     GNQEKPVE"
     gene            complement(18442..18735)
                     /locus_tag="VYO12_001009"
     CDS             complement(18442..18735)
                     /locus_tag="VYO12_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587643.1"
                     /translation="MSKANQLLERIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHFYLESNNGSDADF"
     gene            18700..18921
                     /locus_tag="VYO12_001010"
     CDS             18700..18921
                     /locus_tag="VYO12_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587644.1"
                     /translation="MDSFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19058..19267)
                     /locus_tag="VYO12_001011"
                     /pseudo
     CDS             complement(19058..19267)
                     /locus_tag="VYO12_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
     gene            complement(19388..19530)
                     /locus_tag="VYO12_001012"
                     /pseudo
     CDS             complement(19388..19530)
                     /locus_tag="VYO12_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262445.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(19564..19704)
                     /locus_tag="VYO12_001013"
     CDS             complement(19564..19704)
                     /locus_tag="VYO12_001013"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1587645.1"
                     /translation="MLEITLKSPYQFAHILFQSTIVPHGGHYHFIPESDLSAGELAAA
                     KV"
     gene            complement(19748..20275)
                     /locus_tag="VYO12_001014"
                     /pseudo
     CDS             complement(19748..20275)
                     /locus_tag="VYO12_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5851 a         4258 c         3835 g         6331 t
ORIGIN      
        1 ctatttattt tttgagtttt aaactttggt agaagtcttg aactccttat atttacataa
       61 ctatttgcct ctataactat tatgacacga aaccaacaag caagttcaag cacctccata
      121 tctttattca aatgaggact aagatattca caaactttat cataaacctc tttatctttt
      181 ttcataaaag gggcatacaa cttagcaaat ttatcctaaa attttttgtt gcctttatca
      241 atcatttcaa cactccttta aatataaaaa tgatcctatc aatctttata ttgagcctat
      301 tttctaattg ataccttgca agaaaggaat aatagctgtt cctataccaa gtaggaatgt
      361 aataaatgca gaaagtcata tactgtcatt cttttctatt aaatcatttt gaagtgtctc
      421 aaagcatcca tgatggcttc ttctttttca ggtgtacact gtggaataat ttgtttatcc
      481 tttttagact tgttgtaatg ttctcgtaat tctattccac atttcttttt tatctgtgca
      541 atatataatg tcgaaacttt taattcaaat ttattccaaa catattcttt gatttgagca
      601 tatgttgctt tgctctccgc acttgtcaaa ttcatctcat ccagctcaat ttcaacactt
      661 atgtgcttat cgacatcgag tttggacaaa agtgctaccg tctcgacgtg atgcgtttga
      721 ggaaatagat ccaccggctg gactttcttc aattcatatc ccaattcttg gtagagtttg
      781 atatcacgcg ccatggttgc gacattgcag gagatatagg cgatgcgatc ggctcctgtt
      841 tgggcgcttg ctttgataaa gctttctgtc aagcccttgc gtggaggatc aaccaagata
      901 acggttggtt gaatcccatc tttaagccaa ttcttcatag cattttcagc tgtgtcacag
      961 acatagtggg cgtttgaaat attgttcagc tgagcatttt ttttactatt ctcaaccgct
     1021 tctggaatca gttcaacacc gtagacttct ttgacatgct tggcgactga taaaccaatg
     1081 gttccaatac cagaataggc atcaataatc acgtcatctt tttttaactc tgcaaagtca
     1141 atggctgttt gatagagttt ctccgccatt tcagtattga cttggtaaaa ggctgggcca
     1201 gcgatttgga agtcatttcc caacatctgg tccgtaatat agtcttgacc ataaagagtg
     1261 cgccactcct taccaaaaat cgcattggta ttctggtcgt tgatattttg catgacagac
     1321 acaatctctg ggaactgctt gataacttgt tcaatcaatt ggtcaacacg aaaaactttt
     1381 ggacgagttg tcaccaaaac gaccatgatt tgtcctgaat agtgaccacg acgcaccaca
     1441 agattccgaa tcaatccaga ctgttccttt tcgtcataag gttttaaatc aaaacgacgg
     1501 agcaggtctc gaagagctac tacgacttgg tcaatgacag gatcctggat aaagaaatct
     1561 tcaaggggca tgaggttgtg cgaattctta cggaaaaatc ctgtttccaa gacaccattc
     1621 actcgacgaa cgggcacctg cgccttattg cgatacttga ctggatgttc cataccaagc
     1681 gtttcagcaa cttctacatc tgcaattcca gcaatcttgt agagactgtc cttgacttgc
     1741 ttggttttaa acttgagctg ttctggataa gaaaggtgtc ctaaatccgc gattcctgaa
     1801 cgcaggtaag ccaaatctag atcttgatta cggtgtggtg actggacaag gtatttttca
     1861 acttttccaa agccaatctt tttattgacc ttgaggacac gcatgagaat tttttcactc
     1921 ggtaaagcat tctctacaaa aaagaccaaa ccatctacct tggcaactcc tgccccttca
     1981 tgggtcaagt caacaatttc aacttctaca atatcatttt tctttaacat tcttttctct
     2041 ttctattggc cgacaaaaaa gacggcaacg ttacggttac catccattat accatgaaat
     2101 ttcttaagaa ccaaaactct tgaagaagtt gacaagggct tgccagaggg agcttaagaa
     2161 gttaccaccg tcctctagcg ccttatccgc atcaaagtta aggttgatat ttttaaaact
     2221 gtcgccagct tgtgatacga tgctttgttt aaggtcattt agggttttag tgaaatctgc
     2281 attgctgagg atatcactct ttgagagatt caaggcaaaa ttgatgatga tattgatctg
     2341 gtttcctgtt atgacctgat caagtttgta attttttaag gtatcttcaa caatcttgcg
     2401 tacatcttct tctgtcagat ttcccttgct ttctttagct ttggcgagtc ctgacttgat
     2461 atcagctagg gcaacgttta atttattagc atcatagcct gatttgtcct tgttttcagc
     2521 attgatatct gacaaagcct ttagctcttc ttgagccaaa tctttattgg cttgcggcac
     2581 cttggctcca ttagcttcta gcgaatagta gatcccagcc aaagcactct cacctgtaac
     2641 tggaataggg gctgctacag tgattttggc atgttccata cccagcgtta ctgctgcgtt
     2701 tcggtacata tcctgagtca ccttagtgat attttctggt gtttcaatct tgacctcaag
     2761 tggcgatttg tcacctagct tttgaatctt ggctgatgaa tacaactgta agctagagtc
     2821 attggccaca ttcatgattt tagaataaac atcaggtgtc atggtcttga gttctttggt
     2881 atctgttgag gcattgtagc ccagtttttt aagagtttga tttttttggt cttcagatag
     2941 ggaggaacct aggacatatt caggttggac ataggtttca tcgataactt tttgaacatc
     3001 tgttgctgca tgggcgctat tcatagctgt tactgcccac aagaccgcag cactggtcag
     3061 aaagagtttc tttctcatag ggaatttcct cctttacttt tctagagtaa tatatctatc
     3121 ttaaagaaaa cttataacaa aaacacctgg tctagccaga tgttgaaaag agagtgaaac
     3181 atttgatgat gtaaaggtta agtcgtacct gtctagaata ataatagttt cctccattta
     3241 catagagttc agcaccgtga aaaatggaaa tggggtgaat ataactataa gtctttccag
     3301 tcgtattacc aagcaagggg gcaacagtct cacgagagta ctgtttggct agagccaagg
     3361 tattttcctt gccattttgg gcgataaaat cgatataggc aggtccaaaa ttataggctt
     3421 gaacagctgt ccagatatct acacccttct tctgcgccag atagagattg cctgtcagag
     3481 tttgaatgcc ttgccgaatg ctagaggcat tatcattgat ggtattggtg gaaccacttg
     3541 cagactcact agactgcata acatcgcctt cttttccttt tgtttcagta taaatcatag
     3601 caagcacaag ctcttcgttt gctggggtgt ctttttcact caatatttct cgcaccatgg
     3661 gttgataggt catgacttgt ttgacatctt gatgaacgcg gtaagcttta tatccagcaa
     3721 aaaggaagac tgctagtaca agcactcttc gaattcgttt aaacattatt tactttggat
     3781 atcctcgata tttttgatta agatagagta ggttccattt tcgttttgga taaactcaac
     3841 agactcggcg tcttgataga cgttattggg aacgatgagc tcaattccat ttgataagga
     3901 gagtttttgg ttttcaaatt tctttaattg gcgactggca tcaatttcat caaattgaac
     3961 aggttctggt acggcttctt tgacttggtc aataaagctc aaacgagccg tcagattgtt
     4021 gtcaaaaagg tcattagcca atttctcagg tgacaattca ttgctttctt ctaggttgtt
     4081 gaaaatagct gatttgacct tggattgaaa ttgaaaatca tctgtgttaa aagattcagc
     4141 aattctctgg gctgtttttt ccagttcctt gatagatttt ttaggagaaa tcttaggagc
     4201 gacagcaaga agattatctg aaaaatagtt caaaaaagtc ccgttgtact tgattcgttt
     4261 ttcaatcagg tgatacttgc tactctgaag attgaccacc aaggcctcat cagctcctgt
     4321 tccaaatcca ggcaggttat tctgagttag cttgattgga ttatcaactt ctcctccgag
     4381 gtgggtcaag gtctcccgca gggcaattcg caagaaagcg aaatgttcta caccttcttt
     4441 agaaaattga acaaaaatca agtcattggt cttgagattt tcagaaatgc taaactcctc
     4501 tttccagaga ttagccagcg ttactgatgt ctccaacaaa tcgtctgtaa tatgattgaa
     4561 gaagggattt tcttcttcga aaatcccagt cttggcttca tctgaataca catgttcaat
     4621 ttttttacgt aggtattctt cgatttttgg agtaatattg agaaacttat ctgctaagaa
     4681 cagctcggta tcatccggac tgaactggtg aataatggct ttcttaatat aaatgtccat
     4741 aaaagtttta ttcctcgtat aatgggaagg catctgtcaa ttctttgact gcacttctca
     4801 cttcttctaa tacagcctca ttttctgaat tcttaagggt tttaatgatg agttcagcca
     4861 ctttgcgact ttcttcttca ccaaatccac gtgcagtaat agctgctgct ccgatacgaa
     4921 tcccacttgt cttgaatggt gacaagcttt cgtaagggat tgagttttta tttaaggtaa
     4981 tattgacttc atccaacaag ttttgagcaa ctttgccgtt ttctacaact ttagtcacat
     5041 caacaaggaa gagatggttt tcagttccac ctgaaataat acggaaatca gggtcttgca
     5101 agaagacatc tgccatagcc ttgctgttct taattacatt ggcagcatat tccttgaagg
     5161 ctggatccaa aacttctttg aaggaaactg ccttagccgc cacaacatgc tctaaaggac
     5221 cgccctgaat acctgggaaa atagctgaat tgattttttt agcaagttct tcgtcattgg
     5281 tcaaaatcaa accaccacga ggtccacgaa gggttttgtg ggtcgttgtt gttgtgatat
     5341 gagcgtatgg aactgggctt ggatgaaggc cagccgcaac caagccagcg atatgggcca
     5401 tgtccaccat gagcttcgca ccgacagcat ctgcgatttc acggaatttt gaaaaatcga
     5461 taatttgaga ataggctgaa gcaccagcta caatcagttt tggttttact tcttgggctt
     5521 gtttcaagat agcatcaaag tctaagagtt ccgttttagg atcaacacta taagaaacaa
     5581 agttgtaggt ttgaccagag aagctaacag gagccccatg agtcaaatga ccacctgatg
     5641 ccaaatccat tcccataacc gtatcacctg gctcaatcaa ggacatgtaa gccgcacagt
     5701 tagcttggct tcctgaatgt ggttgaacat tggcaaattt agcaccgaaa atttcttttg
     5761 cgcgttcaat agcaagagtc tctacaacgt ctactacatc agttccacca taataacggc
     5821 gtcctgggta accctcggca tatttatttg tcaagataga cccttgagct gccataacag
     5881 ccttggaaac tacgttttcc gaagcaatta actcgatatt attttgttgg cgttcttctt
     5941 ctttggcaat agcattccag agatcagcat catatgcttt aaaatcatct ttgtcaaaaa
     6001 tcataggtct tctcctttat tgtgtgacta gtccattagt ttgattttac aataagaaaa
     6061 tcaaactaac agatgcgaat aaaccgtttc tgcattttat cacaagtata gccaactttt
     6121 tcataaaatg catgagcacc cagacgatga ttggcagaat ttaagcggat aaacccataa
     6181 ccacatcttt ttgcttcttc ttccaaccct tgtagtaaac ttttaccaat accttgacct
     6241 tgcgcttgag gtgaaactgc taaagctaag atattaaatc ctgctttgga atagagtgat
     6301 tcgtaaactt cagcgtggac atatccaagt aagacatgat tagctgcatc ctcatagcca
     6361 agtaggaaat gatgggaatc ctgagacagt ctagctagtt ggctagccgt ttcctctgga
     6421 ctaaaagtat aacccaaagc ctcttggttg atgtcacata tcgctttcac atctgtttct
     6481 tgcaaatctc ttagcatttc aagcctcctc aaaagaaatc tctgacaacc gagcaagaat
     6541 atcttctcgt ttaattgcgc cttggcgtaa gattttcacc ttgtctccag acaaatccac
     6601 aatagttgaa tcctgtccag ttagaaaagc atcgtcttcc agacccagaa cctcttggtc
     6661 aaaatccttc agaatttgtt caaaggttac accacttgcc tgacctgaga tattggcaga
     6721 cggcccaatc aagggacctg tctctcgaat taaatccagt gtgatagggt gactgggcat
     6781 ccgaaatcca atagttgcaa ggtcagaatt tacccaatag ggaactcggt cattggcttc
     6841 gagaataatg gtcaagggac ctggcaaaaa ggtctctaca agtttttgta gataagctgg
     6901 ctgattcttt gaaaagtgca agatgtcctc gaaagaggcg atattgagat tgagtgcctt
     6961 atctctagga cgacgtttga gttggtaaac atggtcaact gctttttcat ctaaggcctt
     7021 ggaaaaaaga ccataaacag tctctgtagg tagaacgaca gctccaccct tttccaactc
     7081 ttgtctaatc ctgtccatca tcaaccacaa ccatcctatt ttgaccaaat tggtctttga
     7141 gtgttcgaac tcttttttca ggaagatgtt ttctaaaaag ttcgggaaca ctttgacctt
     7201 gcttgtatcc aatttcaagg taaatcttac caccatcctt gagatagtct tttgcatctt
     7261 ccgcaattct acagtaaata gctaggccat cctcgtctgc aaagagagct agatgaggct
     7321 ccgaatgcaa aacattcaag cctacctctg actcatcttc acgagagata tagggtggat
     7381 tggatacaat tatatcatat ttttcagaaa tttctgcaaa acaatcagat tttttaaaaa
     7441 atatattaag attttgatcg ctagcattct ctgatgcaag ctctaaggcc tcttgagaaa
     7501 catctgctgc tgtcactgac caatctggtc tattttttgc tagagcgaga gcaatagctc
     7561 cactacctgt tccaatatcc agaactgaga gattcgtctc aagattttca gttagaataa
     7621 gctccactaa ctcctctgtt tctggacgag gaatcaaaac ccgttcatcc acctttaaat
     7681 gcattccata aaaatctgcc tgtccaatga tgtactgagc tggcttgtga gctgctagtt
     7741 gctggtaaat atcttctaca aattgttttt cttcctctgt tgtcacctcc tgctggaggg
     7801 caaaaataaa gtctgtaaaa gatagatttt tcagactacg atagacaaaa gagaggcttt
     7861 ccgcttcctc tccttgtctt atcaactctt cttcaaaatt tgaaaataat tgagctaatt
     7921 tcattatttg tttaattctt ctagtttttg tgtttggtca taaagcacca aggcatccac
     7981 aacttcgtcc aatttaccag acaaaatcgt atctagtttt tggagggtca agccgatacg
     8041 gtggtctgtg acacggtttt gtgggaagtt ataagttcgg atccgttctg aacggtcacc
     8101 agtaccgatt gtcgacttac gctcagcgtc ttgttcatcc tgagcaatct gagcaaagtg
     8161 gtcagcgacg cgtgcacgga taatcttcat agccttctcg cggttcttct gctgggtacg
     8221 ttcttcctgc atctcaacct tgatattggt tggcaagtga acgatacgaa cggcagtcgc
     8281 aaccttattg acgttctgtc caccagcacc agaggcgtga tagatgtcga cacgaaggtc
     8341 ttttggatca atgtcgtatt caacctcttc aacttctggc ataacaagaa ctgtcgctgt
     8401 cgaagtatga acacggcctt ggctttctgt cacaggaaca cgttgcacac ggtgggcacc
     8461 tgattcatac ttaagcttag agtatacaga ctgacctgaa accatagcaa ccacttcttt
     8521 aaaaccaccg acaccattca tagaggcttc catgacttca aagcgccaac cttgggcttc
     8581 cgcatacttt tggtacatag ttagcaaatc tccagcgaaa agtgccgctt cgtctccacc
     8641 agctgctcca cggatttcaa ggatgatatt cttgtcatcg tttggatcct ttggaaggag
     8701 caaaattttc agtttttctt catattcttc tttttcagcc ttggcatctt tgagttcttg
     8761 cttggccaat tcttccaagt ccgcatctcc gcctgattcc ttaatcatct cttcggcatc
     8821 gacgatattt tgaaggactt gtttatactc acggtaggct attacggtgt cacgattgga
     8881 agcttcttct tttgaaagct ccataaaacg cttggtgtct gaaacgacat cagggtcact
     8941 cagcaattct cctagttctt cataacggtc ttctacagct tgtagttgat catagatgtt
     9001 catttttctt cattctcctt attgatttca ggggcaaaat aatgtttacg gcaaaccgag
     9061 atataggttt cattaccacc aatctggatc tgttctccat cgtaaacggg cagtccatcc
     9121 tgtgttcgca acaccatggt cgcctttttc ttgcaatact gacagatggt cttgatttcg
     9181 tcaatcttgt ccgctaaaag caagagatat ttggaacctt cgaacaattc attgcgaaag
     9241 tcatttttca agccaaaagc catgacgggt atgtctaact cgtccacaac acgagctagg
     9301 tcataaacat ggtggcgttt gagaaactgg gcttcatcga ccaaaacaca gtaaggtttt
     9361 tctggtaggt ctcggatata gccaaagata tccgttgttt cctcaatcgc aagggcaggg
     9421 cgtttcatgc caattcgact cgacacatag ccaacaccgt cacgcgtatc cagagccgag
     9481 gtcataatga caacaccttt tccttgctcc tcgtagttat aggccacttt gagaatctca
     9541 atcgttttac cagagttcat ggtcccataa cgatagtaca actgtgccat gtttcttgct
     9601 tcacgtccat ttctaaattt ttgctacatt ctagtatatc ataattttct taagctttaa
     9661 acggcaaaat gtggtaaaat agaagaaatc aaaaactagt ggaggaagct attatgccat
     9721 ttgtacgcat cgatttattt gaaggacgca cgctcgagca aaagaaagct cttgctaagg
     9781 aagtaacgga agcggttgtt cgcaacactg gagcacctca atctgctgtc catgtcatca
     9841 tcaacgacat gccagaagga acttacttcc cacaagggga aatgcgtacc aaataagcta
     9901 gcttaagcag aattgcttag gctttttcaa tctccaagta gcattcattg aagaaatatc
     9961 ctaaatttgt tacaatttga aaagaaactt ggagaatttc caagaaaaga gcaattaatt
    10021 aaaggaaaca ttatgattac acgtgaattt gataccatcg ctgctatatc tactccacta
    10081 ggtgaagggg ctattggtat tgtccgcctg agcggaacag acagttttgc tattgcgcaa
    10141 aagattttta aaggaaaaga cttgaacaag gttgccagcc acactctcaa ctacggtcac
    10201 attatcgatc ctctgactgg taaagtcatg gacgaggtta tggttggggc tatgaagtct
    10261 ccaaagacct tcactcgtga ggatattatc gagattaaca cccacggtgg gattgcggtg
    10321 accaatgaga ttctccagct agctatccgt gagggagctc ggttggcaga acctggtgaa
    10381 tttaccaaac gtgccttttt aaacggtcgc gtagatttga cacaggccga ggcggtgatg
    10441 gatatcatcc gcgccaagac agacaaggcc atgaacattg cagttaagca attagacggc
    10501 tccctttctg acctcattaa caatacccgt caagaaatcc tcaatacact tgcccaagtt
    10561 gaggtcaata tcgattatcc tgaatatgat gatgttgagg aagctactac tgccgttgtc
    10621 cgtgagaaga ctatggagtt tgagcaattg ctaaccaagc tccttaggac agcccgtcgt
    10681 ggtaaaatcc ttcgtgaagg aatttcaacg gctatcattg gacgtcccaa cgttgggaaa
    10741 tcaagccttc tcaacaacct cttgcgtgag gacaaggcta tcgtaacaga tatcgctggg
    10801 acaacacgag atgtcatcga agagtacgtc aacatcaatg gtgtacctct caaattgatt
    10861 gatacagccg gtattcgtga aacagatgac atagtggaac aaatcggtgt tgagcgttcg
    10921 aaaaaagccc tcaaggaagc cgacttggtt ctactagtgc taaatgccag tgaaccactg
    10981 actgcgcaag acagacaact tcttgaaatt agccaagata ccaatcgcat tattctactt
    11041 aataaaaccg acctgccaga aacgattgaa acttcgaaac tacctgaaga cgttatccgt
    11101 atttcagtcc ttaaaaacca aaacatcgac aagattgaag agcgaatcaa caacctcttc
    11161 tttgaaaatg ctggcttggt cgagcaagat gctacttact tgtcaaacgc ccgtcacatt
    11221 tccctgattg aaaaagcagt tgaaagccta caagccgtta atcaaggtct tgagctgggg
    11281 atgccagttg atttgcttca agttgacttg actcgtactt gggaaatcct cggagaaatc
    11341 actggggatg ctgctccaga tgaactcatc acccaactct ttagccaatt ctgtttagga
    11401 aaataagaaa aatccatgat ccttcattcg gtcatggatt ttattgtctt tattagtaat
    11461 ctggtcttaa gacccctgtt acagttgcct tagttgcttc gtagtcgcca tctacgacaa
    11521 ccttgataat gcgtttggca tcttcttctg gtgctggaac aagaggtaga cgagtgggtc
    11581 cagcttcaaa tcccatatag ttaagaactg ccttaactgg agcaggactt ggataagaga
    11641 agagagcatt aaccttagga atgaatttac gctgaattgc tgcggctttc ttcatatcgc
    11701 tttctgcaat ggcagtaaac atctcgtgca tttcatcccc atttgtatga gaggcaacag
    11761 aaataacccc atccgcccca aggttcatgg catggaaagc atctccatcc tcacctgtat
    11821 aaatcaagaa ctcttcaggc ttgtgctcaa tcaagtaagc catattagcc aagctagtac
    11881 attctttgac accgataata tttggatggt cagccaaacg aagcatggtt tctggagtca
    11941 attcgacaac tacacgccct ggaatgttat agataataat tggtaggtca gaagcatctg
    12001 caatagcctt aaagtgctga tacatccctt cttgagaagg tttgttgtag taaggaacaa
    12061 tagcaagccc agctgcgaaa ccaccaaatt ccgctacttc tttgacaaac tcaatagagt
    12121 cacgcgtatc attagtacct acacccgcaa tcaaaggaac gcgtccattg acaacctttt
    12181 gtacagccgc aaacaactcc aactcctcat cgtgggtcaa agttggactc tcagcagtcg
    12241 ttcctgcgag aagaattcca tccgtatgat gggccaataa atgctcaatc aaggctggaa
    12301 tagcatcaaa gttaatggaa ccatcctcat ggaagggggt aataaaggct gtaatgattt
    12361 tacattcttt taaatcttga taagacacga aaacacctct ctatttcaaa gaaggagttc
    12421 atctgaactc ctaaacgata tgactatttt aattcaaatt tcaattcggc tgttggacga
    12481 accaatccac gttcatgaag agtttcagca atctgaactg agttccaagc agcacctttg
    12541 agaaggttat ctgaaacaac ccacatgtga attccttttt ctgcatccat gtctttacgg
    12601 atacgaccaa caaaggtatc acgcgaacca actgcattga tagcttgagg atagatttga
    12661 tgagctacat catcttcaag aacagcacct gggaaggctg cgatagctgc ttttacttct
    12721 tcgattggag ccacttcttt tgtttcgata taaacagact cagagtgagc tgacaagact
    12781 ggaatacgca cacatgttgc agatactgca atgctatcat cttccataat tttcttagtt
    12841 tccttggtca tcttcatctc ttcgtacgtg taatcattat cagtgaaaac atcaatttgt
    12901 ggaagagcgt taaaggcgat aggataatgt ttcttgtcac cacctgaagg caagatttcc
    12961 gcatgcaaat cacgtggttt cacaccatca ttcaagactt cacgaagttc acgttgtgtc
    13021 tcaagaattg ctcccatacc agcacctgaa acggcttgat aagttgaaac aatgatacgg
    13081 tccaagcccc atttttggcg aaccggctca agagccacca tcatttggat tgttgaacaa
    13141 ttagggcagg caatgattcc gttgtgagca tcaagtgcat gagcattgac ctctggaaca
    13201 accaaaggaa catctggatt ttgacggaaa taagatgtat tatctactac taccacgcca
    13261 gcttttactg cgtatggtgc atacttagct gatgtagaac tacctgctga aaagagagca
    13321 atatcaactc cttcaaaagc tgtttcagtc gtttcttcaa ttgtaatatc ttgatcttta
    13381 aatttcaatg acttacctgc tgaacgtgca gaagcaaggt aacggatttt atcgattgga
    13441 agtgttgatt cttccaacat ttttatcatc tgagcaccga cagcacctgt cgcgccgact
    13501 acagcaactg tatatcccat aaataacctc tttcggaatt ttctaaaaat ttctataata
    13561 gaagtattat actacttttt ccatgaattg caaagctttt tcattatttt ttggaaaata
    13621 aaaatcccag tcgctagact agggactaag aggcatcttc atgtgatgag caggttcaca
    13681 caactcatca aggtccgctc ctgcgttatg acctccttat gctcaatagt agtccgaaga
    13741 ctacctatcg actcatcgca tattctatat tactagagat acttgatttt gtcaataaga
    13801 aattttttcg atcgagtata acaaaaaact cttgtattca agagtttccc tatacatatc
    13861 atgctaattg ccgggattga accggcgacc tcatccttac catggatgcg ctctgccaac
    13921 tgagctaaat cagcttactt aagaagtata ctataatcag aaaaacttgt caagtttcct
    13981 tagaaatttt ccataggaaa aagccaggta agaactacct agcttatctt cttctattta
    14041 cttaaatcaa ttcgacgacc aattttacca ataataatcg ctccaataat caatgaagca
    14101 aattcaccga ttcctgttgt aaaccaagta aggaagaatg gtaattctgc tacaatattt
    14161 aactctgcag cgatagtaaa catggaaatt gaaaagagga ttgaaaagaa gaaatgatct
    14221 tttcgaatca atccgttaaa gaggtaatcc ttgctatatt ttgcaaaaag ccaaacacct
    14281 aaactgagga atactaaggt tgaaccacca ccaacaaaga catctagcag tccgaagcta
    14341 aagaaattag caatcataca accgatggta actccgatga tgtacttagg attgtaaaaa
    14401 gccataaagt tcatcatttc tgaaatacga aactgataag caccatagct gatggcattt
    14461 aggggcggtg tgacagtcaa aacgacgtag atagcagcga cgattgcaat atctgcaaca
    14521 tcacgaatag ttaatttttt catcttttct cctttggcgg ttatccgcgt gtaatatgct
    14581 tggtgaaaga agctaagcac caagggttga ttcattcaac cttactagta tagcacaata
    14641 gcctgcttta tgctatactt aaagcatgaa aaaacctatt caaaaatttt ttaacaatga
    14701 aatcttagcc tatctctttt ttggccttgc gacaactcta gtttcgattc tctcacgact
    14761 agtcatttat caactaagtc atcaggaact ccttgctacg gccttggcaa atattatcgg
    14821 tatcttattt gcctttatca caaatgatac gattgtattt aagcaaaaaa agaaaaatag
    14881 acttatccgt ttagtgaaat tttctcttgc tcgcctttct acttttctgt tagacttgtt
    14941 tttaactttt ctctttgtaa cccaatttcc tcacatcata gggcaattcg taactgaaaa
    15001 cattgataag ataaatgcta ttgaaacaat aattgcacaa atattcatta ttatcatcaa
    15061 ttatatcttg agcaaggtct atatttttag aaaataatgc tccatttatg agccttttaa
    15121 ttgccttcta taatgattta ggatacaatg aaacacgaaa cattgaaagg aaaacagtat
    15181 gttaaaaaat ctaaaatcgt tcttgcttcg aggaaatgtt attgaccttg ctgtcggtgt
    15241 tgtcattgcc tctgcttttg gtgctatcgt tacttcactt gtaaacgaca ttatcactcc
    15301 tcttatttta aatcccgctt tgaaagctgc taaagtcgaa cgtatcgctc aactttcttg
    15361 gcatggagtc ggctatggta actttttaag tgctattatc aattttatct ttgtaggtac
    15421 cgccctcttc tttattatca agggcattga aaaagcacag aaactgactg gcataaagaa
    15481 agaaaaaact gccgaaaaaa aaccaaccga attggaagtc cttcaagaaa taaaagctct
    15541 ccttgagaaa aaataatcga ttaaaaggat ctagcgcaaa gctaaatcct tttttcttta
    15601 ctctttgggt aacttgcctg ctttttcaag catttttttg ataagataag gcatcttaat
    15661 attctcccgc ccttttttct caatcagttt tttcacaaat tctggcattt gtaaatcttc
    15721 agattcagct aaaatgtttt tcgtctcgtc tgaaggaact aactcatcaa aggtctcttc
    15781 ttctgggaga aattccttaa actcttgatt ttcattgact ctaactgtat tgaccaaagc
    15841 atcaatcaaa cgagaatctg tatttggcat tggtggacga tggtagttta ccccaaattc
    15901 ctgacataaa tcataacatt ccacatcatt gtcaaacaag acctcaatat gctcactgat
    15961 gaaactaata ggtacaaaaa tataatggtc tggatgttct gtctgttctc tgagatactc
    16021 caaaacatct ggtttaatcc atggaatacc aatatcactc tcactctgcc aagtgttggt
    16081 atattgttct gaactcaagc ccagtttttc agcgactagc ttgctatttt cgaaaatttg
    16141 gtctatataa ggatctccaa agtccaaggc aaaaataggc acactatggg ctgaaaagat
    16201 gactttaaag ctgtcttgct ttacttcttc ttttaaaatc ttagcaattt catctgccca
    16261 atagtttaag agcgcttctt cctgatacca gtccttaatg accaaaaatt gaatttgttt
    16321 gctttctaaa aatttttcat agcccatgac agagtagaaa gaataatggg gctccaaaat
    16381 caagcaaata cactgttcaa ttccgtctgc ttccatttga ccgatcacat ctggaataaa
    16441 gggactggaa aacttattag caaagtagac actgtattca ttccccagcc tagcttctac
    16501 caaggcaacc tcttgacggg taattttttg cagaggcgtg cctccaattc gtacataatt
    16561 atcatagaga gtctgaatct cgtggtcttg gggtctcatc ccacgacgaa tatttgtgaa
    16621 aaaatcagct acaccttcaa aggtaatctc ttctggtgaa ccgaaggtca tcattaaaat
    16681 tgcttttttc atgactgctt ccttgtgatg tttttatcaa gtttatttta tcattaattc
    16741 atcgataagt aaacgctagc atagggacat tctcaatctt ctgtcttttg tttttctctt
    16801 ctttctatga tacaatggaa aaaataaatt caaaaggagt tttttatgac ttatccaaat
    16861 ctcttggacc gcttcttaac ctacgttaag gtcaacacgc gctctgatga acactctact
    16921 actactccaa gtacacagag tcaggttgac ttcgcaacaa atgtcctaat tcctgaaatg
    16981 aaacgtgttg gactgcaaaa tgtttactat ctaccgaatg gttttgctat tggaaccttg
    17041 ccagccaacg atccgtcttt aacacgtaag attggtttta tatcgcacat ggatactgct
    17101 gattttaatg ctgaaggagt caatccacag gtaattgaaa actacgatgg tggtgtgatt
    17161 gaactaggga attctggttt caaactcgat ccagctgact tcaagagtct tgaaaaatat
    17221 ccaggacaaa cgctcatcac aacagatgga acaagcttgc taggcgctga tgacaaatca
    17281 ggaattgctg aaattatgac agccattgaa tatctaactg ctcatcctga aattaagcac
    17341 tgtgagattc gtgttggttt tggtccagat gaagaaatcg gtgttggtgc caataaattt
    17401 gatgcagaag attttgatgt ggattttgcc tacactgttg atggtggtcc actaggtgaa
    17461 cttcagtacg agactttctc agccgctggt gctgaattgc atttccaagg ccgtaatgtc
    17521 caccctggta ctgccaaagg gcagatggtc aatgcccttc agctagcaat tgattttcat
    17581 aatcaacttc cagaaaatga ccgacctgag ttaactgaag gttaccaagg tttttaccat
    17641 ctaatggatg tgacaggtag tgttgaggag gcgcgtgcaa gctacatcat tcgtgatttt
    17701 gaaaaagatg cctttgaagc gcgtaaagca tccatgcaat ctatcgctga taagatgaat
    17761 gaagaacttg ggagcgaccg tgtcactctc aacttgacag accagtacta caatatgaaa
    17821 gaagtcattg aaaaagatat gactccaatt accattgcta aagccgttat ggaagatcta
    17881 ggtatcacgc ctattatcga accaatccgg ggtggaacag acggctctaa gatttccttt
    17941 atgggaatcc caactccgaa tatctttgca ggtggcgaaa atatgcacgg acgttttgaa
    18001 tacgttagcc ttcagactat ggaacgtgca gttgatacca tcattggcat cgtagcttat
    18061 aaaggctaaa aagacgaggt agctcagcta cttcgccttt ctttttattc tactggtttt
    18121 tcttgatttc cagtagttgt agaagattct gttgttttat tttctgaagt tgattcagca
    18181 ggtttagaat ctcttgtatt gcttggtttg ttttcgtcgc tagcagtttc aatgttagat
    18241 tctgcagttg cgtttggttg gttctcagca ctggtgttat caccatttgc ttcagcattt
    18301 cttgctggac ttgtttcttc acttgcgcta gcttttgact ggatttgatg attcaaaact
    18361 agaatagctt ttgtcgattc aagtaaagct gttttgtctt tactattagc agaaagttga
    18421 tctaataatg catccacctt atcaaaagtc cgcatcagat ccattattac tttctaaata
    18481 aaagtgaagc gacatgagaa tatcgtagag tttttgatag agtacaagtg tctgaggatc
    18541 ttgctcagca ttttcctttt cttgttgaag ggcgctagcg atacgagtca agacatcttt
    18601 tacctgactg tttacttcat ccaagtctgc atcagccttg tttgtggcag cttttagatt
    18661 ttctacttct tctgccaaag attgtctgat tccttcttca tggattcgtt ccaagagttg
    18721 atttgccttg ctcaaaagac tttctacttc ttccttgcta tctgtcgcag attattggtt
    18781 gctatctacc atgtactcct aaaacaggag agttataatc caagattaca aggccttaca
    18841 gaaataagaa atccagataa gacaatgttc gtccaagacg ctattcgctt cgcacagcag
    18901 cacggattca atatgcttta attttaaagt ttaggtgtca agacctcttt ttagtgtgcc
    18961 caaaatttag agaagtaatc aatcaactaa cttttatttt tttcaaactt tcagtaaact
    19021 gacctaaagc taactcaatc tgtctttgtt cgataggctt gtctttgtag atgcttctgc
    19081 tatcagctag aagttgatct acttttgcca agactgcctt ctcatcaaaa gttccaggtt
    19141 gatagttgga ttgcagggat ggaatcttgt ttttcaaagc cgcttcatat cccttagttt
    19201 gaaccttgat gtagtgattg tggtcgccac gaggaatcac aaaaccttct gaatcttcac
    19261 ttataattcg attggcatca aaaccatgac catcttcttc ctcatggtgg acatgtagtg
    19321 acggattact taatacagaa ctagaagaac ttcctacctc ttccgtgtta gagtgtgatg
    19381 ggggattgtt aagagatgac ttaggaatat agtgatagtg accccatgtc ttactatata
    19441 agcatcacct gtatctctga caatatcatt agggttaaag acataaccat catctgctgc
    19501 agaaacacca ttattcggtg tcaccgacaa agattgactg agagctgtag tattctctga
    19561 taattatact tttgcagctg ctaattcacc tgccgacaag tcactctcag gaatgaaatg
    19621 atagtgacca ccatgtggta ctatagtaga ttgaaataga atatgagcaa attgataagg
    19681 ggattttaaa gtaatttcta acaatgattt agaaactatg atgtgctatt ctaaattcaa
    19741 ctcactatat ataaccatca tcggtagtat aacgtccctg taattttact acagatactt
    19801 ctgcactagc tcctttatcg tctttaccat gttcttgttt ttggcgattg atttcatctt
    19861 ttattcgtac attttctgca tgagcttgat ctttaaggta aacataatac tttccatcta
    19921 ccttaataat atatcctccc ttaacctaac tgacgatatc ttgatctttc ggctgatagt
    19981 tgggggcttt cattaatagc tcttcactaa agagcgcatc caaaggaact ttaccattat
    20041 agcagtgata atgatcgcca tgagaagtta cataaccttg atctgtaatc ttaataacaa
    20101 tttgttttgc ttgaattcct tctttttgac taacctagtc tggagtcaaa ttttcagtct
    20161 tcttagtgtc tttattactg tttacatatg aaacacgatt tttatctgta ttggcctgtt
    20221 agctatgttg gttcagagca taaacacaca gacttaagga aaggataaca acaga
//
LOCUS       DAVLXF010000030        19641 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLXF010000030 DAVLXF010000000
VERSION     DAVLXF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.030"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..619)
                     /locus_tag="VYO12_001015"
     CDS             complement(<1..619)
                     /locus_tag="VYO12_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1587646.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KRTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENY"
     gene            complement(728..1246)
                     /locus_tag="VYO12_001016"
     CDS             complement(728..1246)
                     /locus_tag="VYO12_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1587647.1"
                     /translation="MRTYENKEELKAEIEKTFEKYISEFDNIPENLKDKRVDEVDRTP
                     AENFAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1473..2342)
                     /gene="rsmI"
                     /locus_tag="VYO12_001017"
     CDS             complement(1473..2342)
                     /gene="rsmI"
                     /locus_tag="VYO12_001017"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1587648.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2345..2662)
                     /gene="yabA"
                     /locus_tag="VYO12_001018"
     CDS             complement(2345..2662)
                     /gene="yabA"
                     /locus_tag="VYO12_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1587649.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2702..3592)
                     /locus_tag="VYO12_001019"
     CDS             complement(2702..3592)
                     /locus_tag="VYO12_001019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1587650.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3589..4227)
                     /locus_tag="VYO12_001020"
     CDS             complement(3589..4227)
                     /locus_tag="VYO12_001020"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1587651.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4447..5244)
                     /gene="proC"
                     /locus_tag="VYO12_001021"
     CDS             complement(4447..5244)
                     /gene="proC"
                     /locus_tag="VYO12_001021"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1587652.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            complement(5248..6510)
                     /locus_tag="VYO12_001022"
     CDS             complement(5248..6510)
                     /locus_tag="VYO12_001022"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1587653.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASFLPRLEQ
                     VLVAERKEAGLESIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6520..7650)
                     /locus_tag="VYO12_001023"
     CDS             complement(6520..7650)
                     /locus_tag="VYO12_001023"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1587654.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7730..9607)
                     /gene="cbpE"
                     /locus_tag="VYO12_001024"
                     /gene_synonym="pce"
     CDS             complement(7730..9607)
                     /gene="cbpE"
                     /locus_tag="VYO12_001024"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1587655.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAETGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSLISVVKVNG
                     KKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQTS
                     DSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPSFQ
                     AGWHKSVYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFYLT
                     DSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYMGQWYY
                     FAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKANDW
                     YFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKSAT
                     IKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSESE
                     Q"
     gene            complement(9610..10497)
                     /locus_tag="VYO12_001025"
     CDS             complement(9610..10497)
                     /locus_tag="VYO12_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1587656.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLSLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10487..10948)
                     /locus_tag="VYO12_001026"
     CDS             complement(10487..10948)
                     /locus_tag="VYO12_001026"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1587657.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10945..11853)
                     /locus_tag="VYO12_001027"
     CDS             complement(10945..11853)
                     /locus_tag="VYO12_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1587658.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11846..12052
                     /locus_tag="VYO12_001028"
     CDS             11846..12052
                     /locus_tag="VYO12_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1587659.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLHYLPFSR
                     QSYFQNQFKKISGYTPLQYRKEKR"
     gene            complement(12104..12775)
                     /locus_tag="VYO12_001029"
     CDS             complement(12104..12775)
                     /locus_tag="VYO12_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1587660.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12829..13050)
                     /locus_tag="VYO12_001030"
     CDS             complement(12829..13050)
                     /locus_tag="VYO12_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV1587661.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13321..14130
                     /locus_tag="VYO12_001031"
     CDS             13321..14130
                     /locus_tag="VYO12_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1587662.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     NVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14311..15186)
                     /gene="aguB"
                     /locus_tag="VYO12_001032"
     CDS             complement(14311..15186)
                     /gene="aguB"
                     /locus_tag="VYO12_001032"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1587663.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15196..16281)
                     /gene="aguA"
                     /locus_tag="VYO12_001033"
     CDS             complement(15196..16281)
                     /gene="aguA"
                     /locus_tag="VYO12_001033"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1587664.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16278..17405)
                     /gene="nspC"
                     /locus_tag="VYO12_001034"
     CDS             complement(16278..17405)
                     /gene="nspC"
                     /locus_tag="VYO12_001034"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1587665.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17405..18664)
                     /locus_tag="VYO12_001035"
     CDS             complement(17405..18664)
                     /locus_tag="VYO12_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1587666.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(18661..19521)
                     /gene="speE"
                     /locus_tag="VYO12_001036"
     CDS             complement(18661..19521)
                     /gene="speE"
                     /locus_tag="VYO12_001036"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1587667.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDREGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         5667 a         4559 c         3499 g         5916 t
ORIGIN      
        1 tgtaattctc aagctgtgag ttcattatga tttgtaaatc cgatatatct tcagcagcta
       61 aaaacacatt ttgaaatgtt ccaagtccgt tttcaccaaa gctatattct ctcttactat
      121 acttactctt attattctct atatagttag agtcaaggtt cttaacctct tgaagttcat
      181 tttctttact tctgaggtta aaatctttta cttctgaagt taagtttttt gacttctgta
      241 agtcattttc ttttacctga aatatactca taaagtcttt aacataaatg atgttcggct
      301 taccaagtcc aagccttact ctttcgatca gtcctattcc tttttttaca tcaagctcat
      361 ctaatgtttt tatggcagtt ggctttgaga tatttcttct tttcataatt tcttcgacag
      421 taaaatagat aaatactctg ccttccttgt ctatccagtt attcttaaat gacattcctg
      481 tccgtttaag aagtatggaa taaaggataa ttgcttctgc tgataagccc cgaaattctt
      541 ctctatcaac taatatctcc ggtactttta agaagttaaa tctttccgct tctctgttat
      601 aaaaatattc aaagtccatg caagatcacc tcccttctta aaaatttgca aagaaaaaga
      661 cgatagtttt tcttctatcg cctttggtaa ccatttttat gaaatttttt aaagttaaaa
      721 atataattta taatactatc ttcttccatt ttctgatttt agttctgaaa gttccaaaag
      781 gtgcaaccgt atttacatga ataaacttat acacttccca agtcgctgtt ttagtcgctt
      841 catcagccca ctttctcata tgcggttcaa ataattcttc ctcactcaac gaatcaatca
      901 ttgcagagat agaattaata ttttcattta attttgcttt caactcttgc agagataaat
      961 gagcgtaggt atctgtgaac cactgatata attcaccaag ttgattccat ttaaatttat
     1021 ccgatggtgt ttttacttga agcccctttc tttcatcttc ttcccattta agaaccaagt
     1081 tggtccagcc aacctgataa gcaaagtttt ctgctggagt tctatcaact tcatcaactc
     1141 tcttatcttt taaattttct ggaatattat caaattctga aatatatttc tcaaatgttt
     1201 tctctatctc agcttttagt tcttctttat tttcgtatgt tcgcaaaaaa tcaccttctt
     1261 tctgatttat caatcctttg atttaaaatg accttatttt tctatttata ccttctagga
     1321 acgctataac tgctgctcca accatcggta ttccgtatgg gcttaataaa aaacttaata
     1381 ccaaagcttc tataccaagt gatacatctt tatattatca tcactaatta agttaaacag
     1441 aaacagacag aattattaca tcctgtctcc ctttattgtt tttcttccca gtcgtggtag
     1501 gcagcgtaga gctgactttt attccactgg taaatcttag cgacttcctt gatagcttgg
     1561 tttttcttca caccttgctg gatgcgggtt tgaatttcta cgaacaagtc ttcctcgtcc
     1621 ttttcctcca caccctgact ggcaccctca acaatgagaa gacattcgcc cttgagtggc
     1681 gtttcagcaa tgctttctaa taactcagag atagtacctc gttggtattc ttcatagatt
     1741 ttggtcaatt ccctgaccaa gacaactgag cggtcaccgt agacttctaa catattttcc
     1801 aacgtgtctg ctacacgatg aggtgattca taaaaaatct gtgtttcagg ataatctttt
     1861 ttcaagccaa aaaattgctt ctgctgacct gattttctcg gtaaaaaacc gtaaaagata
     1921 tgtggctgtg gcgctaaacc actggcaatc aaggcagaaa ttcctgcaga ggcacctgga
     1981 actgtcacaa ctgcaatttc ttcctcaata gctgccttaa ctaaatcatg accagggtct
     2041 gaaatgctag gcaaaccggc atcagagacc tgagcaatac tttgccctgc tttcaagaaa
     2101 ccaatcaaat caggaatttt ttccttggca ttgtgctcat gaaaactgat ctgcttggtg
     2161 gaaatgtcaa aatgcttgag caaaagacct gtattgcgcg tatcctcagc agcaatccaa
     2221 tctacttctt tcaaggtctg gatagctcga aaggtcatat catctagatt gccaatcggc
     2281 gttgccacta gatacagctt gccataggga gactgcccct taaaactttt ttgaatctgc
     2341 atgcctactc cctgtatagc aactcgtcac aaaacataca ttcctcgtcc tgctctcgac
     2401 gttgtccata aaaatcatta catacgtgaa atccatcacg gtaaatgcga cggacacttt
     2461 cacgaacatg cttggccttg acaggagcat ctgcttccac ctcacccaag cgttctcgca
     2521 acttactatt ttccaagcga agagctgtat tttcctctac caggctcttg agatttttct
     2581 tgatggcttc cacatcggct aaggttacca ataattgttg ggaaaaatca tccagcgcgt
     2641 caaataattc ttttttgtcc ataaaacagc cctttccttt ctttatcatt catttttgag
     2701 tttatatttc tttcaagacc agatattcca tggcattttg aaagctgaca ttagcttgcc
     2761 acatttttct agcttctagt aaatcttgta gaatcactct tactcttacc tgcaagaggt
     2821 cctgcccaca gagaacttca agaatccgta aaacctgatc ctgtttttcc ttatcatctg
     2881 ccaagttggc taatttggca acctgtagat aactttcttt tttcttagct actaaccaag
     2941 tcagcaggcg ttcactttca tcgaccaagg tccaaaaact tgcctgatgt gccaactttt
     3001 ctgcttcagc tcgcgattga ctaaacttag ctaaaagagt cgcttttttc ttaacaagtc
     3061 ccatttgttc taagagtaag ataagttttt cttcttgctt tttaaagtgg aagatctgag
     3121 tccgacttcg gattgtcggt aacatctttt cctcatcgct agtcaagaag aaaatataaa
     3181 cttcactctg gggttcttcg atgaccttga gcagagaatt ggctgcgttg ggatgcattt
     3241 tatccgcttg ctcgatgata aagacctgtt gctggctttc aatccctgct tgagaaaact
     3301 gtcccaccaa ttcccgaatg cgttctgtct tgatgacctg attgactggc ttaatcaagg
     3361 tgacatctgg aaactcttcc tgttcaatca gcttgcaact tcggcatttc tcacatggta
     3421 agacgccaac tttatccgta caaaagaggc tcttagctaa aaattgcgcc atttccaagc
     3481 ttccaaagaa acctgaaaag agataggcgt gattgagctg gtcttgttct aagatacgga
     3541 caaaacggtc aaactgagct ggttgccaag cctttagttg atcttgtttc atttggccaa
     3601 gcccattccg tcaaacaaga cagccttggt agtttccaca acttgctcca aagggagact
     3661 agcatcaatc ttgacaatgc gatttccctc tttatccaga agagaaaggt agccttgacg
     3721 aactttttta tgcaagtcca acccttccaa atccaaacga ttaacctcgc ggtcactatt
     3781 agcagcaata cgagccagcc cttcttccac ctcgatgtca aaatagagtg tcaaatcggg
     3841 tttgaggcca tctgtcgcaa actgattgag ccagtcaatg gcttcaatat ctaagccacg
     3901 accaaatccc tgataggcaa cagaactatc gataaaacga tccatgatga ccaacttgcc
     3961 agcttcaagg gctggaagaa ctttttccac caaatgctgt ctgcgactgg caatatagag
     4021 aagtagctct gttttagcat ccatctgagt atgacttgga tccaaaatca cttcccgaat
     4081 cttctcccca atcaagactc cgccaggttc acgggtcgtc aacacctcta ctcctttttc
     4141 ctctaaaatt ggtagcagag cctctaaaac actggtcttg cctgctccct ctggtccctc
     4201 aagagagact aaaaatcctt ttgacatgtc taactcattt cttttttact acttctattc
     4261 tatcaaaaaa ataggggttt gtgacaatct ttttgtcttc tcatttcata ctcaatgaaa
     4321 atcaaagagc aaactaggaa gctagccgca ggctgtactt gagtacggca aggtgaagct
     4381 gacgcagttt gaatttgatt ttcgaagagt atcaaccacc ataaaagagg cagataaaac
     4441 tacctcttat ttacctagtt cttgtgttcg tttataggct tgatgaactg cctccatgac
     4501 tatgcctcga aaagcatgcg cttctaggct tgctacgcca gcaatagtcg aaccgcctgg
     4561 actacaaact tgatctttca aaactccagg atgttgctgg ctttctagga ccaattgccc
     4621 agctcctacc acagtttggg ctgccatttt caaggctgtt tctcgtggta atcccgtctg
     4681 aacacctgca tctgccaaag cctcaataaa gagatagaca aaggctggtc cacagcctgc
     4741 aagacctgtc gctgcattga ttaagctttc tcctaattca accaagagac cagccttgga
     4801 taaaagctga caaaagagct cactgtcctc agccctgcaa ttaggagaca aggcataact
     4861 aatcactcct tgcccgatag aagcaggggt attaggcatc atacgaataa ttcggtgttg
     4921 acttgggata agacttgcta gtttttctaa ggtcaatcca gctgccatcg aaatcaaaag
     4981 aagactttct cttttttcaa ggatggtctg gtattgagaa agcagttcag aaaactgagc
     5041 aggcttaact cctagaaaaa tcacatctgc ttctgcaaac atttcttcat tgctggaagc
     5101 ctgaccacca aagtctgcaa tgaaagcatc taccttagct tgactacgat tggcaaggag
     5161 aatctcatct gacgtcctag tctgcaagac agattttgcc aagctagcac ccatattccc
     5221 caaaccgata aatccaatct tcatctctta ctcccttatc tgcccatcac cagtgaccac
     5281 atacttgtag ctagtcaatt ccttcaagcc cataggacca cgcgcgtgca atttctgagt
     5341 agaaatcccc atttcacaac caagaccaaa ttgtcctcca tctgtgaaac gagttgaggc
     5401 attaacatac accgctgcag agtccacttg atctgtaaag tatgctgcag cttcagcatt
     5461 ttccgtcaca atggcatccg aatgatgggt actgtgggat tcaatatgcg caaccgcttc
     5521 ttctaaactg ctcacaacct taacagctag aatatagtct aaaaactcgg tatcaaagtc
     5581 ttgtgcttga gcagcttgac ctgaaacaaa ctggcttgct ttgctatcta ggcggaattg
     5641 aattgattcc agtccagctt ccttacgctc tgcaaccaac acttgctcca agcgaggaag
     5701 gaagcttgct gccttgtttt catgaaccag cagaacctcc atggcattac aaacagaagg
     5761 acgactggtt ttagcattgt tgatgataga cagcgccttg tcttcgtctg catccttatc
     5821 cacatagaca tggacaatcc cagtccctgt ctcgataaca ggtacaatcg cattctcaac
     5881 cactgcattg atcaagccag ctcctccacg aggaatgaga aggtctagat agcccttggc
     5941 cttcatcata gcataactac tttcacggct agtatcctcc accagttgaa tcacatttgg
     6001 atgaatagta gtcgtctcca agcccttctt caaggctgtg acaatggcat gggttgtttg
     6061 ataggcatcc ttaccactac gaagaacaac cgcatttcca ctcttaagag tcaaagcagc
     6121 cgcatcagac gtcacatttg gacggctttc atagataata ccgatgacac ccatagctac
     6181 acgttttttt gtgataacca aaccattttc aagctgactt gtttctaaaa cttcaccgat
     6241 tggatctggt aaggcaacca cttcacgaat tcctcttgcc atcgcttcta tacgatctgc
     6301 atccaaataa agacgatcca acatcacatc tgagattttc cccttagccg ctgccatatc
     6361 gagggcatta gccgctaaaa tttcctcagt agcagccact aagtgatcag ccatggctag
     6421 caaggcttgg tttttcactt cttcactagc tgtgttaatc gattttttaa cagcctgtac
     6481 ctgttcaaat tgttcttgtc tactcaccat agtttacctc taaaattctg taaaaagtag
     6541 ttggatttca ggagtaatgg aaatccagtc gtcacggtaa atcaagacac ccttggcttt
     6601 ttgagaacgc aacatatcct ccaaagcaga tgctccaaat tgcacgcgtc cttttccaag
     6661 tgattttcca ctttccttgt caaatactgt cacgatatca ccgtaagaaa agactccttc
     6721 tgcttcaacg ataccagata agagaagact ctttccatgt tgagagagag cttccgcagc
     6781 ccctttatca acccaaatag aaccttgact ctgagcatag aaggcaagcc attgtttctg
     6841 ggtacgaagc cccttctctt gagcaacaaa gtaagaacca tcctcggtct cctctgccgc
     6901 ctcaatcatg gaatctgatt tcaaggatga gcagatataa acaggaactc ctgattccgt
     6961 cgcgatagtt gcagccttga ttttggttaa cataccccca gttccgtttg acgaaccagc
     7021 tccaccagcc atatcaataa tctcacgatt gatggtctcg attctctcca agcgtttggc
     7081 tcttggatct gaattaggat ttccagtata gagaccgtcc acatctgtca agagaaccaa
     7141 aaggtctgct tggaccatcg ccgctacttg ggcacttaga gtgtcattgt ccccaacctt
     7201 gacctcatca ataacgacac tatcattctc attgatgata ggaattgccc cacggttgag
     7261 taaaaccgac aaagcctgat gggcattttt ataacgacgc ttatccacaa agtcatcttg
     7321 ggtcagcaag atttgtgcag aaacgatttg acgcaagaga agattggtcg tatattcttc
     7381 caacaaaagt ccctgcccta ccgctgctga agcctgttta tcagcaatct tagtcggacg
     7441 ctttttaaat cctaaggctc caaaaccagc cgcaatggca cctgaagaca ccaaaatcaa
     7501 ctcatgacca gcctcgtgca gcatagccaa ctgctgggta atatccttta ccttactacg
     7561 tgataaactt ccatcctcat ttgtcagaga agaagtaccc accttaaaga caatccgttt
     7621 gtatttcata gtctcactcc gattattcat atttatccat tataacatga ataacaggaa
     7681 atagaaaaga gttgatagga aaaagaaggc cctaaaacct tcttttctac tactgttctg
     7741 attccgattt gtttacactc gtttcagagc tttgtgaagt tgaggatgtc aaatcttgac
     7801 tagttgaaga atttgtaaca ctttcatgtt gactcgtttc tttattttca agatcctttt
     7861 tcactacttc tttggattct tttatttctg aatgacttgt agttttaatt gtagcagatt
     7921 tctcgatcga aacatccact aaccaggcac cacttgaatc cacagtatag ccttctggtg
     7981 tcttaccatt cacaagtaat tgaccatttt ctttcaagaa gtaccattta tccttgtcct
     8041 tgatccaacc cacagctcgt gaaccgtctg tcttgtagaa ataccaatca tttgcctttt
     8101 tatgccagcc ttgcttcata gctcctgaat cttccagata ataatgttga ccagcaactt
     8161 ctatctcacc tgtcttcatg acaccagtag aatccatata gtaccaggtt tctttatctt
     8221 ttacccagcc cattttcatt tcccctgatg gagcaaagta ataccattgc cccatatatt
     8281 gaagccatcc tgtcttcata gaaccatcaa catccaaata ataccactgc tctttatctt
     8341 gaacccaacc aatttccatc tgattttctt tgttaaaata ataccagatt ccatcaattt
     8401 tcttccaatt tttagcagaa gcaccagagt ctgtcaaata gaaccaatga ttgttccatt
     8461 ttttccattg attctgtaac aagatacccg tttggttaaa gtaataccat tcaccttcga
     8521 tttcattcca accgacagca tactctcctg tagaatcagg cgcttgatac caccagttcc
     8581 catatacact cttatgccaa ccagcttgaa aacttggaat cggcttgtag gatgttgaaa
     8641 tattgacaaa accgtctttt cgaatatcaa aaactgtcgc atcatagtct ttgctggctg
     8701 cgttgattct ctcaattcct cgttctttga gccaattaac atactcacta tcaacaccat
     8761 ttttccaagg tagactatcc gaagtttgaa caatcaaact cggactcaaa tttttaatga
     8821 aatccttggt atttgatttg ttggtatcat ggtgatgatt aaacttcatc aaatcaactt
     8881 ttccaatgag aggaccatac ttgtcttctg ctccatgaac attatctaaa tcgcccccaa
     8941 ggtaaatttt cttgccattg actttcacca cgctaatcaa ggaattgtca tcccaaattt
     9001 tctttaattc acccgatgaa tcagtttcat tttcataatt atagagctga atatccatgt
     9061 ccccaaactg aaaatgagca tccccttgtg tgatattttg aataactgaa acacctgttt
     9121 ctgcagcagt ctgtaaaacc ttatcatagc catacagatt atcccataga cgttcagaat
     9181 tagtaatacg actatcacta tatttcttaa gatagactcg gtcaactgga taggtagaca
     9241 gtaattcatc aacatttcca atatgatcac tgtgggtatg ggtcaccaaa ataaaatcaa
     9301 gtttttggac acccaattcc ttcaaacgac gaaagacacg gtctgttaga acatgcttat
     9361 aagacgtttc aattccttct ctccatggat agcgagaatc acttccatct gggaaatcat
     9421 aatcttctcc tgtatccacc atggcaaaat gtccattgct ttcaagaata atcgcatcac
     9481 tgccaccttc ttgaacattg ataaagtgga ttttatttcc tgaactttct tgagcctgaa
     9541 cattcccata ccatgacaaa cctaaaaaag cgcctacaag tgctaaacta gttaatttct
     9601 ttttcattct tactttctca atctctccaa ggtttcctta aaaatctctg ggatatctgc
     9661 tttaaattcc aaggtcttac ctgttctcgg atgagtaaaa cctaaagtct tggcatgaag
     9721 aaattgtcca tgtcctttca aagtcttgcg aggaccatag acctcatcac cagcgactgg
     9781 atggccgata taagccatgt ggacacggat ttgatgagtg cgccctgtct ccagttgcaa
     9841 ctctactaag ctataatcgc caaagcgttc caagacgtga aaacgcgtca ctgcaggctt
     9901 ccctttagca gttacagcct gtttcttacg gtctttttca ctccggccaa tcggcgcttc
     9961 aattacacca cgatcattag gcagatttcc atgaacaatc gcccaatatt tgcggagaga
    10021 ctttttatcc ttgagttctt gggcaagtgc tagatgcgca tcatcgtttt tagcaatcat
    10081 gagaagacct gacgtatcct tatcaatacg gtgaacaatc cctggacgca gaaccccatt
    10141 gatacccgac aagtccttaa tatgatacat gagggcattt actagggttc cactggtatg
    10201 accagcactc gggtgcacaa ccattccctg aggtttgtta acgacagcca catcctcatc
    10261 ttggtagact atttctagcg aaagatcctc agccacatac tctaatacct ctggttctgg
    10321 cacatggtaa gtgacgacat caccctcttg gactgtgtat ttagctttct tgacttgacc
    10381 attgaccaag acctggcctg atttaatttg ttcattcgcg agactacgtg ataattctga
    10441 caaatctgac aaagccttat ccaaacgcag accaccagtt tcaattttaa tttccattta
    10501 tttcctcttt tagcattgca atcaataaaa taatcactcc aaccgtcaga tagctatctg
    10561 ccacattgaa aattgcaaag ttgataaagt caaggtggaa catatccaca acaaagccct
    10621 gactgaccct gtcaataaag tttccaagac cacccgcgat tattagagtc aaacccaaga
    10681 ccatccagaa tgagtcctcc atgtgtttat gtaaatacca aatggcacct atcacgacaa
    10741 ccagagtaat gacagcgaat aacagctgct gatcttgtaa gatagaaaag gctgcacctc
    10801 gattttgcag gtaggtcaag ctaacgaaat tgggaatcca ggagcgcact tcacccagtg
    10861 gaatctgctg gacgatatag gatttgacca actgatccag cccaattaaa agcagtacaa
    10921 tgactgccac tattgctctt tttttcatga tttcctcttt tgatcaaaat attcttgcat
    10981 gacttctacg aagagagtcc cagcttgact aagctccact tcttcacgtt taacatagac
    11041 catgcggtta tctaggttat ccttgagacg aataactgta atgccattaa cactgtcact
    11101 atctaaaaat ccagaacctg tcgcataggc gtccgtccgc tccaaaatac cattcaaggt
    11161 ggcacggtct gtcacattaa acatctgtga gctagcgctg gtatcgacaa agttctctga
    11221 ataataaagg tactcgtctt tctcttgagt gaaacgaacc gttggtaaat ccgctaaatc
    11281 ctccatgact aattcctctt tctgggctaa aggatgaccc tcacagagat aaatatgggt
    11341 atggaaagga atcaattcga tgacctccag acctaacttt tcaacccgtt gcataatccc
    11401 ctttttattt tgattgttga ggtagataat cccaatctca ctatgccctt gcgccacttc
    11461 atctaatatt tgaacagtag ttgattcaaa aatacggaag ttcttatagt caggatagcg
    11521 ctctgaaaag gccgtaatag ttggtggtaa gaagtcatag tgctggctag caacggaaaa
    11581 ttcatctttt tcttcttcag gattggcata ctgattttga aaaatatcaa atcctttaac
    11641 caattcttgc gctttttcat aaaattccat cccacgacgg gtcaagaaag tccctgagct
    11701 ggtccgacgg aaaatcttaa agcccaactc tttttccaaa tcacgaacag aaatagacag
    11761 actcggctga ctaacataca tcttttcagc agcttcacga aaagtaccac tattggcaat
    11821 agccacaaca tagcgtaatt gttgaatgtt catcttctac ccccaacttc tttatctttt
    11881 cattatacca tattttagaa gttttccaaa aaggaaaaaa gaacatccta ttcttcttaa
    11941 ctatcttcac tatcttcact atctgccttt ctcacgccaa tcttattttc aaaatcaatt
    12001 caaaaaaata agtggctaca caccacttca gtatcggaaa gaaaaacgtt gactatttgt
    12061 gaaaaaagaa aatgccggaa aattccgaca tttttttagt aagctaactt cctgaaaata
    12121 ggagtaacca aacatcccag aaggcagtga tattgatgag aatatgaact agtattggat
    12181 agtagagatt tttagtcatg cgatacaata gagctaaaat cagacctcca ctagcataaa
    12241 tgaaaaaagc aagaggagtt aaagcaaaat tgatatgaat ataaccgaaa ataatagcag
    12301 aaagcaaaac atctccgtac caaggtgagt ttttgaaaaa ggttgtcata agcacacctc
    12361 gataaatcaa ttcctcagca ataggggcga tgaagcaaac gatgagcaag aaataaggga
    12421 actcctgtct ccccatcatt tctaccgttt cattcaaaga aatctgattt gaagacaggg
    12481 atatgaaata cgaaaagagg aagtcagaca tatatgaaat gatgtagccc agtaaaaggt
    12541 agatgaagta cctcagctgc cacttgaaat gaaaaatttt ctttcctgca aaaaccagta
    12601 gatagatgac cgctaagaaa agaactccgc tctccatcaa aagcagaatc tgaaaaaatt
    12661 cacgacttgc tggtagatag ggctttgcga ggtgattgaa aactctccaa aaccaagttg
    12721 atttgtaaaa aattaaggac aatgctagta aaatttgaat agcccgtttt ttcatattaa
    12781 atcctctgtt ttctcccctt gtttcttcat attcctaata aattttactt aaaatctcgc
    12841 agcacttcct ttgcaaagat gattgcctcc tccaatatat cctaaatcat aggtgcctct
    12901 gggacaaaat ccatgaaccg gcagagttaa tactggttct atttcccaat cgtacctacc
    12961 attaggattt ttaccatagg gatctgctcc accttgtatc agctctaatt cttcatttgt
    13021 tagatttata ttttttgtca ttaaaatcat gatatatttc ctccattatt cttcagatta
    13081 gttgagtaat ctactactca tacctactta caaaaaaact attttaagct agttttttta
    13141 tgcttgtcta gttgcaatat tgacaagttt agtatatcat atctttgcca aatatcctct
    13201 tcaaatcttg aattgtcatc gaaacgtcct aaactgcaaa atcaattagt ctcaagcttt
    13261 ctatcaattt cttctccaaa atatgctata ataatagcaa aagataaaga aggaagacct
    13321 atgattaaac tactagcctt ggatatggac ggcaccctcc tcaatgaagc caaggaaatc
    13381 ccacaagctc acattactgc tattcacaaa gccattgaaa aaggtgtcaa actggttctc
    13441 tgtacgggtc gccccctttt cggtgtcctc ccctactaca aaaaactggg gcttgacctc
    13501 cagaacgaat atgttattgt taacaacggt tgttcaactc accaaaccag tgattggggt
    13561 ctagttgact ggaaagaact gagtccagct gacatcgaat acctctatga ccttgctgaa
    13621 aagagtaatg ttcagttgac actttttgac gagtcacatt attttgttct cggtggcaag
    13681 cccaatcaag ttattgaaaa tgatgctaaa ctagtatttt cagacctgac tgaaatttct
    13741 cttgaggaag cgactagtgg aaagttccgg atgttccaag gtatgttttt aggaacaaaa
    13801 gaacaaacag acgattttga gcagcgtttt gctgaagagc tttgccaacg attcagtgga
    13861 gttcgttcgc agcctgtcat ttatgaagca atgccacttg gaacgacaaa ggctactgct
    13921 ctttcacgac tagctgagat tttgaagatt gattcctcag agattatggc catgggcgat
    13981 gctaataacg atatcgaaat gctccagttt gcagggcttg ggattgcaat gggaaatgcc
    14041 agcgattatg tcaaatctct tgcggatgcc gttacctcaa gcaacgaaga agacggcgtt
    14101 gcgcgtgcta ttgagaaata tattctataa aaaagaaaag caaatagaca gaagttactg
    14161 ctatttgctt ttttgctaat attttaaaat acggactaat caatagacaa gaatagcgaa
    14221 tcataaaaat cgatttacta gatgccatag tatcttatag ttgctaataa gaagttagtc
    14281 tagcagaatg aatctctcat ttctcccaca ctaatctgta atttgtctat acatttctgg
    14341 tcttctatct cgaaacaagc cccagtttag gcgttcactc gctcccttgt ctaggtcata
    14401 agtagctaac agaacagctt cttcttgtct ttcagctcgt tctagaatag ctcctgtttc
    14461 atccgtcata aaggaggaac cgtagaagtc aagactggaa ctctgtccgc cattttcctc
    14521 actaggagta acctcctcta aaccataacg attggctgcg atgactggaa caatattcgc
    14581 tgctgcgtgc ccttgcatag tacgttgcca gtgaccacaa ctatctgtat ccaaaatcgg
    14641 ctctgaaccg atggctgttg gataaaagag caattctgcc ccattcagag caagacagcg
    14701 ggctgtttcg gggaaccatt gatcccaaca gataccgata ccaatcttag catagcgagt
    14761 attccagacc ttgaaaccag tgttaccagg cgtgaaatag aatttttctt gataataatg
    14821 gtcatctggt atgtgggtct ttcgataaac gcccagcact tccccatctg catcaataac
    14881 ggcaatagag ttatacaaga cattaccatc tttttcatag aaactgattg gtaaaacaac
    14941 ttgtagttcc ttagcaatca ccttaaaatg ctgaatggca gtattttccg ctacagattg
    15001 ggcatactgg tagtagtcat actgacgttc ctgacagaaa tagggatgtt caaacaactc
    15061 gggcaagaga ataatttggg ctccttgctc agcagcctga cgtactaaac gctctgcggt
    15121 ttggatattt gttgccacat ccttagcgca ttgcatctga atggttgcaa ctcttacatt
    15181 tctcatcttt ttctcctatt ctggaatttg ttgggtgata cagtggatat tgccaccacc
    15241 taagagaata tctctggctg gtattccgac aactttacgg tctgggaaac acttgctgag
    15301 gatatctaag gccacttggt cgtttacatc ctcaaactgt ggaaccaaga cagccttgtt
    15361 ggcgatataa aagtttacgt aggaagctgc tagtcgttca cctgcgtatc gcttttcttc
    15421 tccttcttca tagatgtagc ctggcaaatc ttcttctgtc acaacttgtc gaactgcagg
    15481 gataggcaat ttatgaatgg tgaagtgaca accttttgca tctgtttcct gttctaagag
    15541 ttcgagatct gcttttgaca tggcatactg gggatcattt tcgtcatctg tccaagctaa
    15601 aacaacctca gcaggaccaa caaaggcagc aacattatcg acgtgttcat tggtttcatc
    15661 ctgataaata ccataaggaa gccaaataac tttttcagca ccaagacttt ctaataatgt
    15721 gttttcaatc tcctctttag tcaggttagg attgcgacca ggactaagca agcaactttc
    15781 agttacgaga atagttcctt gaccatcgct atggattgcg cctccttcca gtacaaaagg
    15841 tttagcatca tagacaggct tttccaaggc ctcagcaaaa cgactggcta cttggtcatc
    15901 ctcttcataa tcttgataaa gaccatcata ggtgcctccc caagcattga aggcccaatc
    15961 cacggctaat ttcttacctt tatcattgac gagaatggtt gggccagtat cacgcgccca
    16021 ggcatcattg gtgggaatgt ctaaataaac aaccttgtct ccaagatagg attgggcttc
    16081 agatagatag gcctgctcca ccaaaagata gactctttcc ccttctgcta tggtctcgat
    16141 gatctgagta aatgatcttt tagcagcctt tccttgaaaa ggccatgatc ctggtcgagt
    16201 cggccatatc atgagggtac catgatgggg ttcgtactct gctggcatgt gatagcctaa
    16261 tttttttgga ctgtccatca tgataatctc cctttaaagt cttgatagcc aaaagctttg
    16321 agtaagctac agtctccctg ttcgtccatg agatagagac ttggcaatcc aataccatta
    16381 aaggtattat ttttgacaaa agaataaatg gccatgtctt gaaaataaag tctgtctccg
    16441 atttggactg gattttcaaa actataatca ccaatcacat cgcccgtcag acaggtatta
    16501 gaagaaagtc tgtaggtatg ggctttttcc tgtgcctcaa agccatttct caaaggtgga
    16561 cgatagggca tctcaagtac atcaggcata tggcaggtcg cagaggcgtc taaaaccaag
    16621 atttccatac cattttctac aatatctaat acctcagttg ctaaataacc cgcattaagc
    16681 gcaatggctt caccaggctc gatatagatt tcaagattgt aagtttttcg gatacgcttg
    16741 atttctgaaa tcagcaaatc cacatcgtaa ccttctcttg taatatgatg accaccaccc
    16801 atattgagcc attttacctc atgtaagtag ggaccaaact gttcttctac tgctttcaaa
    16861 gttgtttgta aatcatctgc tccctgctcg caaagggtat gaaaatgaag tccgtcaacc
    16921 aaatctagca aatcactcgg aatcttgtct atagtaactc caaagcgaga accaggtgca
    16981 caagggtcat agagcgcgtg atctccttga gttgaacact gagggttgag gcgcaaacca
    17041 acactgacac cagcctctcg acaacgagcc ccatgcttac gcaactgtct ctctgagtta
    17101 aagacgatat ggtctgttat cccaagcaat tcctccatat cctcatcctt gaaagctggt
    17161 gcaaaaacat ggacttcacc aggaaattct tcccttgcca atttggcctc atagagtcca
    17221 ctagctgtcg tacctgatag atactggcta atcaagggat aagttttgta gagggaatat
    17281 gccttctggg caagcaagac cttgcaaccg gcctcttctt gtacatattg tagaatgcgg
    17341 caattagctt ctaacttggc caagtcaata acataggctg gtgttggtac ttgttctaac
    17401 ttcattagtc caccatttgt ggattttcaa ccacaaccca tggcaaacca tactcattca
    17461 aagcttccat gaatggatct ggatctaact cctcaaggtt atacactcca gcttgtttcc
    17521 aagttccgtt catgactaat tttgtcccaa tcatggctgg aactcctgtc gtataagaaa
    17581 tagcttgcga accaacctct gcgtaacatt cctgatggtc gcagacattg tagatataga
    17641 tagtcttttt gacaccgtct ttgacacctg taaagataca tccaatattg gttttaccta
    17701 cagtacgtgg accaaggcta gcaggatctg gaagcaaggc tttcaaaaat tgaattggaa
    17761 caatttcttg gccgttaaag ttaatggtat ccgtacgaag gagtccaaca ttttcaagac
    17821 atttcatgtg cgtcaagtaa gattgaccaa aagtcataaa gaaacgaatg cgtttgactc
    17881 caggaatgtt cttggccagc gattcgattt cttcatggtg aaggagatac atatcttttt
    17941 gtccaacttg agggaaatca tactcacgct tgatagacat agcttcgact tcgacccatt
    18001 tcccatcttc ccagtaagaa cctggcgcag aaacctcacg gagattaatt tctggattaa
    18061 agttggttgc aaatggataa ccgtggtcac cgccattaca gtctaaaatg tcgatataat
    18121 ggatttcatc aaaatagtgt ttgagggcat aagctgaaaa gacactagtt acacctgggt
    18181 caaaaccaga accaagaaga gcagtcaagc ctgcttcttt gaatttctct tgataagccc
    18241 actgccatga gtagtcaaag taggctgtaa aaccaagttc cttacaacgt ttttcgtaga
    18301 tagcacgcca ctcagggtct tctgtgtctt ctgcttcgta gttggctgta tcgatatagt
    18361 gaacacctgt tgccaaacaa gcatccataa tggttaaatc ttgataaggc agagctacat
    18421 tcaaaacagc ttctggtttg tagctttcaa tcagggcaat cacttcttca accttgtcag
    18481 catcaagtgc tgcagtttca attttagtac ttgttttgcc ttctagcttc gctttcaagt
    18541 catcgcattt tgacttggta cggctagcaa tcataatctc tgtaaatgtt tcgctatctt
    18601 gacaaatctt tgaaatagca acttgggcaa cgcccccaca accaataact agtaaacgac
    18661 tcattttttt ccttcctctt cttctaaaat gtcctcaaca tacttgggca acataaagga
    18721 tcccacgtgt aagtttgcag tgtagtattc tgtgaaaagc tggcgttttt tccaaccttc
    18781 cctgtcaaaa tctttgacag ggtggtattt tttcgatgca aatccaaaca accaatagcc
    18841 agctgggcta gttggaatat gggcctgata aacccgactg attggaaagg cttgattgac
    18901 cttgcggtgc atgcttcggc aggccgactc atcctcgtca aagaagggac tcccatgctg
    18961 gtaaatcatg atgccgtctt ccttcagagc tcgataacta ttgccgtaga attctttggt
    19021 aaagagtcct tccgtatggc caaatggatc tgtcgcatcg ttgatgataa tatcgtaatc
    19081 atcttcgcag tttcgcaaaa agcgtagccc attttggtag taaatggtaa cacgaggatc
    19141 atctagccct gcagcaaagt ctgggaaata ctcacgacag acctcgacca acatttcatc
    19201 cggttccacg atatcgattt gttccagttc aggatagagt gttaatactt gggcaacacc
    19261 gccgtcacca cccccaataa ccaatacttt ctttggattt gggtggacag ccatgggaac
    19321 gtggacggtc atttcattgt agacgaaatc atccgcatct gagaacaaga catggccatt
    19381 taaaatcagt attttcccaa aagctggcgt atccaagact tcgatatcct gccattcact
    19441 ttttccagcg taaagttgct tggctgttct cagagacaat ttgacatctg gagtatgaac
    19501 ttcagaaaac cataaatcca tctatttctc cttatattat atttagcgca gttagctact
    19561 gccaaagccc aagtggtata cttggaataa gccactgtgg attagttcat ttcctccaat
    19621 cgcttgacga tttccgaaag g
//
LOCUS       DAVLXF010000031        19112 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLXF010000031 DAVLXF010000000
VERSION     DAVLXF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19112
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.031"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            150..843
                     /locus_tag="VYO12_001037"
                     /pseudo
     CDS             150..843
                     /locus_tag="VYO12_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            897..1286
                     /locus_tag="VYO12_001038"
     CDS             897..1286
                     /locus_tag="VYO12_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1587668.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(1947..2975)
                     /gene="queA"
                     /locus_tag="VYO12_001039"
     CDS             complement(1947..2975)
                     /gene="queA"
                     /locus_tag="VYO12_001039"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1587669.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            3126..3833
                     /locus_tag="VYO12_001040"
     CDS             3126..3833
                     /locus_tag="VYO12_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1587670.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            3911..4420
                     /locus_tag="VYO12_001041"
     CDS             3911..4420
                     /locus_tag="VYO12_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587671.1"
                     /translation="MNKEEFLKIKEAYKSARTEERKSIIGFITKKKDKEGNFLFTKSK
                     DKPYTTRNQYSGGGGNKKYTSGSRLSRPYDLSNHMWIDLSYKGNDILISLQSFDIDPN
                     SKELHVLYDRIGILFEQSKKIPIFKDCYTITKVSDAFLKMETTNWELPLSKADMEEMV
                     NYIINHYEE"
     gene            4500..4676
                     /locus_tag="VYO12_001042"
     CDS             4500..4676
                     /locus_tag="VYO12_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1587672.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            4806..5741
                     /locus_tag="VYO12_001043"
     CDS             4806..5741
                     /locus_tag="VYO12_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1587673.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINIADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            5734..6522
                     /locus_tag="VYO12_001044"
     CDS             5734..6522
                     /locus_tag="VYO12_001044"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1587674.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            6523..6906
                     /locus_tag="VYO12_001045"
     CDS             6523..6906
                     /locus_tag="VYO12_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1587675.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            6922..7311
                     /locus_tag="VYO12_001046"
     CDS             6922..7311
                     /locus_tag="VYO12_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1587676.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIHITEE"
     gene            7395..8525
                     /locus_tag="VYO12_001047"
     CDS             7395..8525
                     /locus_tag="VYO12_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1587677.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            8530..9267
                     /locus_tag="VYO12_001048"
     CDS             8530..9267
                     /locus_tag="VYO12_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1587678.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            9278..10051
                     /locus_tag="VYO12_001049"
     CDS             9278..10051
                     /locus_tag="VYO12_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1587679.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            10041..10658
                     /locus_tag="VYO12_001050"
     CDS             10041..10658
                     /locus_tag="VYO12_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1587680.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            10868..11269
                     /locus_tag="VYO12_001051"
     CDS             10868..11269
                     /locus_tag="VYO12_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1587681.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            11269..11547
                     /locus_tag="VYO12_001052"
     CDS             11269..11547
                     /locus_tag="VYO12_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1587682.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            11537..12310
                     /locus_tag="VYO12_001053"
     CDS             11537..12310
                     /locus_tag="VYO12_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1587683.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSSTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            12311..13615
                     /locus_tag="VYO12_001054"
     CDS             12311..13615
                     /locus_tag="VYO12_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1587684.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            14189..15067
                     /gene="pstS"
                     /locus_tag="VYO12_001055"
     CDS             14189..15067
                     /gene="pstS"
                     /locus_tag="VYO12_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1587685.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            15067..15984
                     /gene="pstC"
                     /locus_tag="VYO12_001056"
     CDS             15067..15984
                     /gene="pstC"
                     /locus_tag="VYO12_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1587686.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            15974..16858
                     /gene="pstA"
                     /locus_tag="VYO12_001057"
     CDS             15974..16858
                     /gene="pstA"
                     /locus_tag="VYO12_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1587687.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            16869..17672
                     /gene="pstB"
                     /locus_tag="VYO12_001058"
     CDS             16869..17672
                     /gene="pstB"
                     /locus_tag="VYO12_001058"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1587688.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKDFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            17685..18443
                     /gene="pstB"
                     /locus_tag="VYO12_001059"
     CDS             17685..18443
                     /gene="pstB"
                     /locus_tag="VYO12_001059"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1587689.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            18455..19108
                     /gene="phoU"
                     /locus_tag="VYO12_001060"
     CDS             18455..19108
                     /gene="phoU"
                     /locus_tag="VYO12_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1587690.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
BASE COUNT         5608 a         3477 c         4390 g         5637 t
ORIGIN      
        1 tatattgttt aacgtttgat agaaaagatg ctatactaga agtaggatgt aatggcaaaa
       61 tctaatacta ataagcgcta tgcaatacgt aaactcggaa agatttttgg ttctgcggta
      121 atcgggctaa ctttattttt aggaggaatc tgaatacaag ttttgttcat gctgctgatg
      181 ggattcaata tgtcagagat gatactagag ataaagaaga gggaatagag tatgatgacg
      241 ctgacaatgg ggatattatt gtaaaagtag cgactaaacc taaggtagta accaagaaaa
      301 tttcaagtac gcgaattcgt tatgaaaaag atgaaacaaa agaccgtagt gaaaatcctg
      361 ttacaattga tggagaggat ggctatgtaa ctacgacaag gacctacgat gttaatccag
      421 agactggtta tgttaccgaa caggttactg ttgatagaaa agaagccacg gatacagtta
      481 tcaaagttcc agctaaaagc aaggttgaag aagttcttgt tccatttgct actaaatatg
      541 aagcagacaa tgacctttct gcaggacagg agcaagagat tactctagga aagaatggga
      601 aaacagttac aacgataact tataatgtag atggaaagag tggacaagta actgagagta
      661 ctttaagtca aaaaaaaaga ctctcaaaca agagttgtta aaaaaggaac caagccccaa
      721 gttcttgtcc aagaaattcc aatcgaaaca aaatatctcg atggcccaac tcttgataaa
      781 agtcaagaag tagaagaagt aggagaaatt ggtaaattac tcttactaca atctatactg
      841 tagatgaacg tgatggaaca attgaagaaa ctacttctcg tcaaattact aaagagatgg
      901 taaaaagacg tataaggaga gggacgagag aacctgaaaa agttgttgtt cctgagcaat
      961 catctattcc ttcgtatcct gtatctgtta catctaacca aggaacagat gtagcagtag
     1021 aaccagctaa agcagttgct ccaacaacag actggaaaca agaaaatggt atgtggtatt
     1081 tttataatac tgatggttcc atggcaacag gttgggtaca agttaatggt tcatggtact
     1141 acctcaacag caacggttct atgaaagtca atcaatggtt ccaagttggt ggtaaatggt
     1201 attatgtaaa tgcatcgggt gagttagcgg tcaatacaag tatagatggc tatagagtca
     1261 atgataatgg tgaatgggtg cgttaacaga ttcctccctg cgacaaaatt gataatagta
     1321 tgtctgaata tataattata tataaaaagc tatagataag gtcatcttat agcgactata
     1381 aaaaagtgaa caagcctagt cttctgtaca ttagaattca gatttgttca ctttttgttt
     1441 aatactcaat gaaaatcaaa gagcaaacta gaaaactagt cgcaggttgc tcaaagtact
     1501 gctttgaggt tgtagataga actgacgaag tcagtcacat atataatcca aggtgacgtt
     1561 gacgcggttt gaagagattt ttgaagagta taagtctttg attttgagac taaggagagt
     1621 cagggtcaaa gtagtgaatg attctgaaat aactcataat agtctcttga gaggacagat
     1681 aagagtaaca aatatggtgc tactcatgtg tcttgcattt tgatttacaa acagcgaaga
     1741 aaatatagga agctgaaata aaatatagat gaatcaattt ggagagtcaa cgcaatctat
     1801 ggcaatattt tcgaagctag ggggaagcat tttaatttca atgtactagg cctcttctac
     1861 agctagctag agtttcaatt gtagatgttc tttgaaataa tatcttagcg atttattggt
     1921 attagaagat ttagagaaat tctttctcaa taaataaaca tggcgtcacc aaaactgaag
     1981 aagcggtagt gttcttggat ggcatggtgg taggcatcta agactaattc acggcctgca
     2041 aaggcagaaa ccaacatgac cagagttgat tttggcaggt ggaagttggt tgagaaggca
     2101 tccacgacct tccactcata cccaggtttg ataaagatat tggtccaacc agaatctgct
     2161 tggatttgcc catcaaactt ggaaccaata gtttccaagg tgcggataga agtggttccg
     2221 acagcgatga cacgaccacc attttctttg acagagcgaa gggtggcagc agcttcctca
     2281 gaaagttgat agaactctga gtgcatttcg tgttcgtcca gattatccac agaaacaggt
     2341 ctaaaggttc cgagtccgac atggagagtc agatagacta gatgaacacc cttagcttgg
     2401 atttctgcca gcagttcttt ggtgaagtga agaccagcag tcggtgctgc agcagagccg
     2461 ctttccttgg cgtagacggt ttgataacgt tcacggtcat ctaatttttc gtggatataa
     2521 ggtggcagag gcatttctcc cagactttcc aagacttcta ggaaaattcc ttggtattca
     2581 aagcggacaa tgcgtccccc gtgggtcaat tcttctgtaa cgacagcgct gaggcggcca
     2641 tcaccaaagc tgatacgagt accgaccttg aggcgtttgg caggtttagc cagaacttcc
     2701 cactcgtctc cactagtgtt cttaaggagg agaagttcca catgacctcc tgtctccact
     2761 ttttgaccat agaggcgggc agggagaact cgggtgtcgt tcatgacaag ggcatcacca
     2821 ggttccagca tatcaataat agagtggaag tgtttatctt gcatttctcc tgtctcacgg
     2881 ttgacgatga ggagtttgga ggcatcacgt ttttcaaggg gcgtttgggc aatcaattcc
     2941 tctggcaagt ggaaatcaaa atcagctgta ttcatatatt tgttcattct ttctaagttc
     3001 taatcctatc cattataaca cgtttcaagt ttggacgctc tttttttaga aattaaatcg
     3061 ttttcacttg acaaaaattg gtctatacca tataataaat atagacagaa atggaggatg
     3121 aaaagatgaa agttattaaa gttgaaaacc aagttcaagg tggaaaagtt gcttttgaga
     3181 ttttgaagga aaaattggct aatggagctc aaactctagg acttgcgaca ggaagtagcc
     3241 cacttgaatt ttacaaggaa attgttgaga gtgaccttga tttttcaaat ctaaccagtg
     3301 tcaaccttga tgagtatgtg ggccttgatg gggacaatcc acagtcttat cgttacttca
     3361 tgcaagaaaa cttgttcaac caaaaaccat ttaaagaaag tttcttgcct cgtggtgtta
     3421 aggacaatgc tgaagctgaa gttgaacgct acaaccaaat tttggctgac catccagttg
     3481 acctccaaat cttgggaatc ggtcgcaatg ggcatatcgg ctttaatgag cctggtactc
     3541 catttgacag tcaaacgcat ctagtagaac ttgaccagtc tactatcgaa gccaatgcac
     3601 gcttctttgc caagattgaa gacgtcccaa cccaagctat ttcaatgggg attaaaaaca
     3661 ttttggatgc caagtctatt attctttttg cttacggtga gtcgaaagca gaagccattg
     3721 ccggaacagt atctggccca gtgactgaga acctacctgc aagtagcctc caaaatcacc
     3781 ctgatgtaac catcatcgca gatgctgaag cgcttagctt actcgaaaaa taaaaagtat
     3841 aagaaccgct tgctctttgg agtgagtggt tttagacata taaaacttca acttaacttt
     3901 aggaggacac atgaataaag aagaattttt aaaaataaaa gaagcatata agagtgctag
     3961 aacggaagaa agaaaaagta taataggctt cattacaaaa aagaaagata aggaggggaa
     4021 ttttcttttt actaagtcaa aagataaacc atataccaca agaaatcaat attcaggagg
     4081 cggaggaaat aaaaagtaca cctcgggtag tagattatct aggccatatg atttatcaaa
     4141 tcacatgtgg attgatttgt catataaagg aaatgacatc ttaatctctt tacagtcttt
     4201 tgatattgat ccaaatagta aagaacttca cgtcttatat gatagaatag gcatcttatt
     4261 tgaacaatct aaaaaaattc caatttttaa agattgctat acaataacta aagtcagtga
     4321 tgcttttctt aaaatggaaa caacaaactg ggaattacct ttatctaaag cagatatgga
     4381 agagatggtg aactatatta ttaatcatta tgaagaataa aaaacttagt ttcctcttga
     4441 caattattcc ttttacgtgt agaatgaaat agatattgaa cttgaaggga gtgaaaaaaa
     4501 tgtctaaaac agtagtacgt aagaatgaat ctcttgacga cgcacttcgc cgtttcaaac
     4561 gtgcggttac taaagctggt actcttcaag aaacacgcaa acgtgaattc tatgaaaaac
     4621 cttctgtaaa acgtaaacgt aaatcagaag tagctcgtaa acgtaaaaaa ttctaattga
     4681 aatgaaaggc tagagaaatc tagtcctttt tcttttaaat aaatactcca aagcctgcaa
     4741 aaatctgaaa cttcctccta caatttgata taatagagag aagaactcat ttgaaggagg
     4801 aaatgatgtc ggttttagta aaagaagtga ttgaaaagct tagactagac attgtctatg
     4861 gtgaaccaga attgcttgaa aaggaaatca atatagcgga tattacgcga cctggtcttg
     4921 aaatgacagg ctattttgac tactatacac cagagcggat tcaacttttg gggatgaagg
     4981 agtggtctta tctgatcagc atgccttcca acagccgtta tgaagttttg aaaaaaatgt
     5041 ttctacctga gacaccagca gtcattgttg cccgtggttt ggtggttcca gaggagatgt
     5101 taaaggctgc tagagaatgt aagattgcta ttttaaccag ccgtgcagct accagtcgtt
     5161 tatctggaga gttatctagc tatctggatt ctcgtttggc agaacgtacc agtgtgcacg
     5221 gtgtcttgat ggatatttat gggatgggcg tcttgattca gggagatagt ggaattggta
     5281 agagtgagac aggtcttgag cttgtcaaac gtggtcaccg tttggtagcc gatgaccgtg
     5341 tcgatatctt tgccaaggat gagattactc tctggggtga accagctgaa attttgaaac
     5401 acttgattga aattcgtggg gttggtatta tcgatgttat gagtctctac ggtgcgagtg
     5461 ctgtcaagga ttcttcacag gttcagcttg ctgtctattt ggaaaattac gatacgcata
     5521 agacctttga tcgtcttgga aacaatgcag aggaacttga agtttctggc gtagccattc
     5581 ctcgtattcg tattccagtt aaaacaggtc gtaatatctc tgttgtgatt gaggcagctg
     5641 ccatgaatta tcgtgccaag gaaatgggct ttgatgcgac ccgtttgttc gacgaacgac
     5701 tgacaagtct catcgctcga aatgaggtgc aaaatgcttg atccaattgc tattcaacta
     5761 ggacccctag ccattcgttg gtatgcctta tgtattgtga caggcttgat tcttgcggtt
     5821 tatttgacca tgaaagaagc acctagaaag aagatcatac cagacgatat tttagatttt
     5881 atcttagtag cctttccctt ggctatttta ggagctcgtc tctactatgt tattttccga
     5941 tttgattact atagtcagaa tttaggagag atttttgcca tttggaatgg tggtttggcc
     6001 atttacggtg gtttgataac tggggctctt gtgctctata tctttgctga ccgtaaactc
     6061 atcaatactt gggattttct agatattgcg gcgcctagcg ttatgattgc tcaaagtttg
     6121 gggcgttggg gtaatttctt taaccaagaa gcttatggtg caacagtgga taatctggat
     6181 tatctacctg gctttatccg tgaccagatg tatattgagg ggagctaccg tcaaccgact
     6241 ttcctttatg agtctctatg gaatctgctt ggctttgcct tgattctgat ttttagacgg
     6301 aaatggaaga gtctcagacg aggtcatatc acggcctttt acttgatttg gtatggtttc
     6361 ggtcgtatgg tcatcgaagg tatgcgaaca gatagtctca tgttcttcgg acttcgagtg
     6421 tcccaatggc tgtcagttgt ccttatcggt ctcggtataa tgatcgttat ttatcaaaat
     6481 cgaaagaagg ccccttacta tattacagag gaggaaaact aaatgttaga agttgcatat
     6541 attcttgttg ccttagcttt gattgtcttt ttggtctatc tgatcattac tgtacaaaag
     6601 cttggtcgtg tcatcgatga aacagaaaag acgattaaaa ccttgacttc agatgtggat
     6661 gtgaccttgc atcacaccaa tgagttgttg gctaaggtca atgtcttggc agatgatatc
     6721 aatgtcaagg tggctacgat tgatccactc ttcagtgctg ttgcagattt atctctatct
     6781 gtttcagacc tcaatgacca tgcgcgtgtc ttgagcaaga aagcttcatc agctggttca
     6841 aaaacactca agactggtgc aagtctgtca gctcttcgtc ttgcaagtaa atttttcaaa
     6901 aaataaaaaa ggagaatcct tatgggtaaa ttatcctcaa tccttttagg aacggtttca
     6961 ggtgcagctc ttgccttgtt tttaacaagt gataagggca aacaagtttg cagtcaggct
     7021 caagattttc tagatgattt gagagaagat ccggagtatg ccaaggagca agtctgtgaa
     7081 aaactgacag aagttaagga gcaggctaca gattttgttc tgaaaacaaa agaacaggtt
     7141 gagtcaggtg aaatcactgt ggacagtata cttgctcaag ctaaatccta tgcttttcaa
     7201 gcgacagaag catcaaaaaa tcaattaaat aatctcaagg aacaatggca agaaaaagcc
     7261 gaagctcttg atgactcaga agagattgtg attcatataa cagaagaata aaccatcacc
     7321 atctccggac ggactatgta tctggggatg gtgattttta tctggaatct agtctttgtg
     7381 gtataataat tactatgcag aaaaaaccaa cgtcagccta tgtgcacatc ccattttgta
     7441 cccagatttg ttattattgt gatttttcaa aggtcttcat caaaaatcag ccagtcgaca
     7501 gctatttaga gcatctgctg gaagagtttc gttcttatga tattgaaaag ttgtcaaccc
     7561 tttatatcgg tggtggaaca ccgacagccc tgtcggctcc gcaactggag gtgttactga
     7621 atggcttgac taaaaacttg gatttgtctg tcttggaaga gttgaccatt gaagccaatc
     7681 caggcgattt ggatgcggat aagatagctg ttttgaaaaa ttcggctgtc aatcgtgttt
     7741 cgctaggtgt ccagaccttt gatgataaga tgttgaaaaa gattgggcgc agtcatttgg
     7801 agaaggatat ttatgaaaat atcgatcgcc tgaaactggc tggttttgac aacatctcca
     7861 ttgatttgat ttatgctctg cctggtcaga ccatggagca agtaaaggaa aatgtggcta
     7921 aagccattgg attggatatt ccccacatga gtttgtatag tttgatttta gaaaaccata
     7981 cggtctttat gaaccggatg cgacgtggga aattgcctct gcctaaggag gaactagaag
     8041 cggagatgtt tgagtacatc attgcagagc tggagcgagc gggttttgag cattatgaga
     8101 tttctaattt ctccaaaccc ggttttgaaa gtcgtcataa tctcatgtac tgggacaatg
     8161 ctgaatacta tggtattggt gcaggggcat ctggttatgt caatggagta cgctataaaa
     8221 atcatggtcc cattcgtcat tatctcagtg cggttgagga aggcaatgct tgtattacag
     8281 aagatcacct gagtcaaaag gagcaaatgg aagaagaaat gttcttggga ctccgcaaga
     8341 aatccggggt ttccatggcg cgatttgagg aaaaatttgg acagtctttt gctggacttt
     8401 atggagaaat tgtcagagat ttggttcaac aaggcctcat gcagattgag ggtgaccacg
     8461 ttcgcatgac aaagagaggt ctctttttgg gagatactgt agcagaacga tttattttgg
     8521 agtaggatca tgggcttaac ttatcaaatg aaaatgaaaa ttccttttga tatggctgat
     8581 atgaacggtc atatcaaact tccagatgtg attttgctgt cactgcaagt ttcagggatg
     8641 caatcgattg aactgggagt tagtgataag gccattttgg aagactataa tctggtctgg
     8701 attatcgcag aatatgatat tgaggtggtt cgtttgcctc gttttgcgga agaaattacc
     8761 atcgaaacgg aagccttgag ctacaatcga cttttttgct accgtcgctt tactatttat
     8821 aatgaagcgg ggcaggagct catccatatg atggcgacct ttgttctcat ggaccgcgac
     8881 agtcgaaaag tccatgctgt tgaacctgag attgtggctc cttaccagtc tgattttgat
     8941 aaaaaactta tccgtggacc gaagtatgaa tccttggaag agccgatcag caaggattac
     9001 catgttcgtt tttacgactt ggatatgaat ggtcatgtca ataacagtaa atacttggac
     9061 tggatttttg aggtcatggg agcggatttt ttgacccact atattcccaa gaaaatcaac
     9121 ctcaagtatg tcaaggaagt tcgaccaggt ggggtgatta catcggctgt tgaacggact
     9181 ggactggaaa gcaagcatga aattacaagt gatggggtta tcaatgccca agcaatcatt
     9241 acttggcaag aaataaaaaa ggtttaggga gaaatagatg aaatataaag gctatttaat
     9301 cgatttagac ggaaccattt ataaggggaa agaccgaatc cctgctggag agacttttgt
     9361 tcatgaattg caaaagcggg acattcccta tctctttgta accaacaata caacccgcac
     9421 tccagagagt gttaaggaga tgttggctca gaattttaat atcgatacgc ctctatcgac
     9481 tgtctacaca gcgactttgg caaccatcga ctatatgaat gatttggggc ttgaaaagac
     9541 cgtctatgtc gtcggagaag caggactcaa ggaagccatc aaggcggctg gttatgtgga
     9601 agacaaggaa aaccctgcct acgtggtagt tggtctggat tggcaagtcg actatgaaaa
     9661 atttgccaca gcaactcttg ctattcaaaa gggtgcccat tttatcggaa ccaatcctga
     9721 cctcaacatc ccgacggaac gtggtctttt gccaggtgct ggctcactga ttacgctgct
     9781 tgaagtagcg acacgagtga agccggttta tatcggaaaa ccaaatgcca tcattatgga
     9841 caaggcggtt gagcacttag gtttggaacg tgaagagtta atcatggttg gggacaatta
     9901 tttgactgat atccgggctg ggattgacaa tggcattcca acactcttgg tgacgacagg
     9961 ttttaccaag gcagaagaag tagctggcct gccaatcgca ccgacacatg tggtttctag
    10021 ccttgcggag tgggattttg atgaaaacta aattgacctt ttggggctct atgctctttc
    10081 tcctctccct ctcaatcctt ctgaccattt atctggcttg gattttctat cctatggaga
    10141 ttcagtggct aaacttaacg aatcgagtct atctaaaacc agaaaccatt caatacaatt
    10201 ttcatatctt gatgaattat ctgaccaatc cttttagtca ggtcttacag atgcctgatt
    10261 ttcgttcgtc agcagctggt ctgcaccatt tcgcagtggt caagaatctc tttcatttgg
    10321 ttcagctagt agctctagtg acactgccaa gtttctatgt ctttgtcaat aggattgtga
    10381 aaaaggactt tttgtctctt tatcgaaaaa gtctcctgac tctagtagtc ttacctgtga
    10441 tgattggact tgggggagtt ttgattggtt ttgaccaatt ctttactctt ttccatcaaa
    10501 ttctctttgt gggagatgat acctggcttt ttgatccagc caaggatcct gttattatga
    10561 ttttgccaga gaccttcttt cttcatgcct tcctcctctt ttttgccctc tatgaaagct
    10621 tctttgggta tctgtgtctg aaaagtcgta ggaagtgaaa tgaaagatgt ttttaatttg
    10681 catatataaa aatatatatc gtttaaaacc atcgttaaga gtgaattaac caaatccaat
    10741 tgtgtcaatc gttcgcaaac agcctatttt tcttgaaaaa ataggctttt tttctaaaaa
    10801 tccctagtca agcgccttat ttttttgtat aatagaagta gcaaagtata gataagaaga
    10861 gaaaagcatg attacactat ttctatcacc gagctgtaca tcatgtcgta aggctaaggc
    10921 ctggttagaa aaacataagg ttccctttgt ggaacacaat attatgacca gtcctttaac
    10981 aagaaaagaa ttgcaacaca tcctttcctt gaccgaaaat ggtactgatg acatcatttc
    11041 aactcgttca aaaatttttc aaaaattgaa tattgatgta gagagtattt cggtatcgga
    11101 attgcttcat ttgattgagc agtatcctag tcttttgcgt cgtccaatta ttattgatgc
    11161 caagcgtatg caaatcggtt ttaatgaaga tgagattcgt gcttttctcc ctcgtagtta
    11221 tcgtaagcaa gaactaaaag aagcaagaat gagagctggt attagttaat gaacaaacag
    11281 tatagttacc cactagattt gtcgtggagc actgaagaac ttgcttcagt gctttctttt
    11341 tttaatgatg ttgaagctgc ctatgaaggc aaagtagagg ctaaaaagtt actggactcc
    11401 tataagggat tcaaagcggt cgtgccaagc aagagcgaag agaaacgttt gggacgagaa
    11461 tttgaaacgg ttagtggcta ttcactttac cgagcagttc aggctgccaa agaaaaaggg
    11521 gaaggaaaga tttctcttgg aaagtaaatt tgaatttgct aaagagcttg ttaagaaagc
    11581 aggccagtac attcttgacc atatgcagga agacttgcgt gttgaaacca agtcctcttc
    11641 aacagatttg gtgaccagac tggacaaaga agttcaggaa ctcttggttg gtgagatttt
    11701 gtcccgttat cctgaggata agatttgtgc tgaagaaggt tgtctgcgag cctcggttca
    11761 agagggcaag gtttgggtca ttgatcccat tgatggtacc aataattttg tagcccagca
    11821 ggaagatttt gctgttatga tggcttattt tgaaaatggt cagggacagt ttggtctgat
    11881 ttatgatgtg gtcaaagggg attgttacca cggtggtggg gaatttccag tttgtctaaa
    11941 tgataggccc ctagctcctt ttaaaactaa accacttgga gattttctca ttgcagggaa
    12001 tagtggtatg ctggaaacca atgaatgggg gctggctgat ttgagccgat cggttcttgg
    12061 tgttcgtgtc tatggaagtg cggccattag ttttgccaag attttgtcag ggcgtttgtt
    12121 gacctatctc acttatctgc aaccatggga ttatgccgca gctagtattt taggggaaag
    12181 tctgggctat cgggttgtga ccctttttgg tgaagctcct gattttcaaa ccagacagcc
    12241 ggtcatgatg gtgcctcttg agatgcagga ggaaattcag tcctatattt acgaaaggaa
    12301 aagaacttaa atgcaatttc cagaaggatt tgttgaaaaa tataaagaga tactaggaga
    12361 tgaggcaaga gattttcttg cctcttttga ggaggaagcg gtttcggcct ttcgggtcaa
    12421 tcccttaaaa gaagagcaac tttccttttc tgatgccatt actcaaaccc cttggggcca
    12481 ctatgggaag gtttcaggga aatcgcctga gcatgctaca ggtttagttt attcgcaaga
    12541 acctgctgct caaatggtgg ctcaagtagc ccaacccagt cctggtatga aggtcttgga
    12601 cttggctgct gcaccggggg gcaaatcaac tcaactggca gcctatctag caggtgaggg
    12661 gctacttgtt tccaatgaaa tttcaagcaa acgggctaag attttggttg aaaacatgga
    12721 gcgttttgga gcgacaaatg tcgtggtgac caatgaatct gccgaccgct tggtcaaggt
    12781 ctttaaaggc tattttgact taatcgttct tgatgcccct tgctctggtg aaggaatgtt
    12841 tcgtaagcaa cctgatgcta tggactattg gagcttagat tatccgagtc aatgtgctag
    12901 cttgcaaaga gaaattctgg aggatgcagt gaccatgtta gctgaaggtg gtcgtctggt
    12961 ttattcgacc tgtacctggg cacccgagga aaacgaagag attgtcaatt ggttgctgga
    13021 agagtatgat tttgacttgt tgccagtaga gcatatcaat ggaatggtag ctggtattga
    13081 cctgccagaa acggctcgga tgtaccctca tcagtttaag ggagagggtc agtttgttgc
    13141 ccatctacag tttaagggta acaatccagc tcctaaattt aaggcaagta agagtaacct
    13201 cagccgtgaa caagttgcct tgtggcagga atttgcccaa aaccatttga aggtcaatct
    13261 accgggtatc ttgcagactt ttggagacca gctttatctc ttgccagaac ttttgccaga
    13321 tttagggaag ctcaagattg ctcgcaatgg actgcatctg ggcactttta agaagaaacg
    13381 ctttgagccc agtttcgctc ttggtttagc cttgaaaccg agtcaggtcg aacagtcggt
    13441 tgaaattggc caagaagcgt ttgtcaagta tgcggctgga gaaaccgttc agctggcaga
    13501 aagtctgcca aatggttggt accaagtttt ggttaaaggc aatggcttgg gctttgcaaa
    13561 ggttactgga aatgttttga aaaattattt tccaaaaggc ctcagattca aatgaaaatg
    13621 ctagtcaaat atcttattct atgttatagt gaaagtatgg atttttttag taggatgatt
    13681 cttgatatct tgattgccat cacatgtagt tttactactc gatgaaaatc aaagagcaaa
    13741 ctaggaagct agccatagat agctcaaaac actggtttga agagattttc aaagagtaca
    13801 agaagtttag ttattagcgt tcttaccgct tgtaaactag atttctcata aaatagaatc
    13861 ttttcctttt agttgtaaac tagtctggga gagtagagag gtttgagata cctttctagc
    13921 ttttggatta tcatctgagg agagtaattt cccttgcatt aaaaagggga aaaagagaca
    13981 cgaaatgatt ataatgggtg acaatggggg aagggataga caagagattt tatccacata
    14041 tgaaaaaagg aggttaggaa agagtaatat agcctatatt atataaataa tcaattgcgc
    14101 agaaatttgg taagaattca tgcgtcaact cataaagaac tacttaaaaa attcacagta
    14161 ttcataatta ttttcgagga gaaaaacagt gaaaaaaaga aaaaagcttg ctctgtctct
    14221 tatcgctttt tggctgacgg cttgtttagt aggctgtgct agctggattg atcgtggaga
    14281 atccataacg gctgttggct caactgcctt gcaacccttg gttgaagtag cggcagatga
    14341 atttggcacg atccatgttg gaaaaacggt caatgtccaa gggggaggtt ctggtacagg
    14401 cttgtcccag gttcagtctg gggcagttga tataggaaac tcagatgtat ttgctgagga
    14461 aaaagacgga attgatgctt ctgctcttgt tgaccacaag gtcgcggtag ctggcttggc
    14521 tctgattgtc aataaggagg ttgatgttga taacctaacg acagagcaac ttcgtcaaat
    14581 cttcataggt gaggtaacca attggaaaga ggttggtggt aaggacttac ccatctctgt
    14641 tatcaatcgg gcagccggct ctggttctcg tgctaccttt gatactgtca ttatggaagg
    14701 tcagtctgcc atgcaaagtc aggagcagga ttcaaatgga gcggtaaaat caatcgtatc
    14761 aaaaagtcca ggagctatct cttatttatc tcttacctat atagatgatt cggtcaaaag
    14821 catgaagttg aatggctatg acttaagtcc agaaaatata agtagcaata attggccctt
    14881 gtggtcttat gagcatatgt atacattggg gcagcccaat gagttggctg cagaatttct
    14941 caattttgtt ctctcggatg agacccaaga agggattgtc aaaggattga agtatattcc
    15001 gattaaggaa atgaaggttg aaaaagatgc tgccggaact gtgacagtgt tggaagggag
    15061 acaataatga atcaagaaga attagctaag aaaatgttgc ttccatcaaa gaattctcgt
    15121 ctggagaaat taggaaaagg tttgaccttt gcctgtcttt ctttgatagt catccttgtg
    15181 gccatgattt tggttttcgt agcgcaaaaa ggcttgtcga ccttctttgt caatggtgtg
    15241 aatatctttg actttctttt gggaggaact tggaatcctt ctagtaaaga atttggtgcc
    15301 cttcctatga ttttgggttc ctttatcgtt accattctct cagcccttat cgcaacaccc
    15361 tttgctattg gtgcagcagt ttttatgacc gaagtatcac caaaaggggc gaagattttg
    15421 caaccagcta ttgaactcct ggttgggatt ccttcagtag tgtatggatt tattggcttg
    15481 caagtcgtcg ttccctttgt tcgcagtgtc tttggtggga ctggttttgg gattttgtca
    15541 gggatttccg tcctctttgt catgattttg ccgaccgtaa cctttatgac aacggatagc
    15601 ttgcgtgcgg ttcctcgtta ttatcgtgaa gccagtttcg ctatgggagc cactcgctgg
    15661 cagactatct ggcgtgtgat cttgaaggcg gcccgttctg gtattttcac tgcagtggtc
    15721 tttgggatgg cgcgtgcctt tggtgaggct ttagctatcc agatggttgt cggaaactca
    15781 gctgttatcc caacttcctt gaccacacca gctgcaactt taacttctat attaactatg
    15841 ggaattggga acactgtcat gggaactgta aataataatg ttctctggtc actggccttg
    15901 gtactgctct tgatgagttt agtttttaac agtgtgatta aattgattac gaaagaaaga
    15961 ggaaagaaaa attatgcgcg ctaagaaatt agataaactt gcaacagctg tcctctatac
    16021 gattgctagc atcattgtga caatcttggc ttccttgatt ctctatatct tggttcgggg
    16081 cttgccccat atctcttggt ctttcttgac tggaaggtct tctgcttttc aagcaggtgg
    16141 tgggattggc attcagcttt acaattcctt tttcctattg gtcattacct tgattatttc
    16201 tgtacctctt tctatgggag ctgggattta cttggctgaa tatgctaaaa aaggtcctgt
    16261 taccaacttt gtgcggactt gtattgaaat cttgtcctct ttaccatcag tggtggtggg
    16321 tctctttggt tacttgatct ttgtagtcca gtttgagtat ggattttcaa tcatttcagg
    16381 tgccttggcc ttgacagtct ttaacttgcc tcagatgacg cgtaatgtag aggatagttt
    16441 gaaacacgtt caccataccc aacgtgaggc tggtctggct cttgggattt ctcgctggga
    16501 gacagtggtt catgttgtta ttccagaagc gcttccaggt attgtaacgg gtgtcgtctt
    16561 ggcatctggt cgtatctttg gcgaagctgc agctctgatc tatacagcag ggcaatcggc
    16621 gccagctctt gactggtcta actggaatat cctcagtgtg actagcccca tctctatctt
    16681 ccgtcaagca gaaaccttgg ctgtccatat ctggaaagtc aatagtgaag gcactattcc
    16741 agatggaacc attgtatcag caggttctgc cgctgtgctc ctgatcttta tcctgatttt
    16801 taactttgga gctcgtaagt tcggaagcta tctacacaag aaattaaccg ctgcctaaag
    16861 gagaagccat gtcaacatat aactgggatg agaagcatat ccttaccttt cctgaagaaa
    16921 aagtagccct ttctactaag gatgtccatg tttactatgg taaaaatgaa tccattaagg
    16981 ggattgatat gcaatttgaa agaaataaaa ttacagcttt gattggtccg tcgggatcgg
    17041 ggaaatctac ctacttacgc agtctcaatc gcatgaatga taccattgat attgctaaag
    17101 taactgggca gattctctat cgtggaattg atgtcaaccg tccagaaatc aacgtttatg
    17161 aaatgcgtaa acacattgga atggtttttc aacgccccaa tccatttgct aagtcaattt
    17221 accgtaatat tacctttgcg catgaacgtg ctggagttaa ggataagcaa gtcctagatg
    17281 aaatcgtaga aacctccctt cgtcaggctg ccctctggga tcaggttaaa gacgatctcc
    17341 acaagtcagc cttgacctta tcaggtggtc agcaacaacg tctctgtatc gctcgtgcca
    17401 tctctgttaa gccagatatc ctcttaatgg atgagccagc ttcagccttg gatccgattg
    17461 cgaccatgca actagaagag accatgtttg agctcaagaa agactttacc atcatcattg
    17521 taacgcataa tatgcagcag gctgctcgtg caagtgacta tacaggcttc ttttacttgg
    17581 gtgatttgat tgagtatgac aagactgcaa ctattttcca aaatgccaag ctacagtcca
    17641 ccaatgacta tgtatctggt cactttggtt agaaaggaaa ccgtatgaca gatgcgattt
    17701 tacaggtatc agacctgtcc gtttattata ataaaaagaa ggctttgaat agtgtttccc
    17761 tatctttcca acctaaggaa attacagcct tgattggtcc atctggatca gggaagtcaa
    17821 ccctcctcaa gtctctcaac cgcatgggag atctcaatcc agaggtgacc acaactggat
    17881 ccgtggtgta caatggtcac aacatctaca gtccgcgtac agatacggtt gaattacgta
    17941 aggaaatcgg aatggttttc caacaaccta atcctttccc tatgactatc tatgagaatg
    18001 ttgtctacgg gcttcgtatc aatggaatta aggataagca ggttctggat gaagccgtag
    18061 aaaaagcctt gcaaggtgcc tctatctggg atgaggtcaa ggatcgtcta tatgattcag
    18121 ctattggatt gtcaggtggt caacagcagc gtgtctgcgt ggcccgtgtc ttggcaacta
    18181 gtcctaaaat catcctcttg gatgagccaa cttcggcttt ggatccgatt tcagctggta
    18241 aaattgagga aaccttgtat ggtctaaaag acaagtacac catgcttctg gtaacccgtt
    18301 ccatgcagca agcttcacgt atctctgata agacaggatt tttcctagat ggagatttga
    18361 ttgaatttaa tgataccaag cagatgttcc ttgatcccca acacaaggaa acggaagact
    18421 atattacagg aaaatttgga taaggagatg aaagatgtta cgatctcaat ttgaagaaga
    18481 tttagagaaa ttacataacc agttctacgc tatgggacaa gaagtgctct cacaaatcaa
    18541 tcgtacggta cgtgcttttg tcacgcatga ccgtgacctg gcaaaagagg tcatcgaaga
    18601 tgatgcagaa gtaaatgaat acgaagtgaa actggaaaag aaatcatttg aaatgatcgc
    18661 actccaacaa ccagtctctc aagatttgcg tacagtcttg actgtcctta aggctgtatc
    18721 agatgtggag cgtatggggg atcacgctgt agccattgcc caggcaacca tccgtatgaa
    18781 gggggaagaa cgcatcccag ctgtagagga agaaattaaa aaaatgggac gtgaagttaa
    18841 aagcgttgtt gaagcagcac ttgatcttta tcttaatggt tctgttgacg acgcataccg
    18901 ggtggcctcc atggatgagc aaattaacca ctattttgaa actatccgtg accttgcgac
    18961 tgaagagatt aagaaaaatc cagaagccat tgttacgggt cgtgattatt tccaagttat
    19021 ttcctacttg gagcgtatcg gtgactatgc taaaaatatc tgtgaatggg ttgtctactt
    19081 tgaaacaggt aagattgtcg aactataaaa ta
//
LOCUS       DAVLXF010000032        19052 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLXF010000032 DAVLXF010000000
VERSION     DAVLXF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.032"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            352..1398
                     /gene="pheS"
                     /locus_tag="VYO12_001061"
     CDS             352..1398
                     /gene="pheS"
                     /locus_tag="VYO12_001061"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1587691.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1398..1907
                     /locus_tag="VYO12_001062"
     CDS             1398..1907
                     /locus_tag="VYO12_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1587692.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1984..4389
                     /locus_tag="VYO12_001063"
     CDS             1984..4389
                     /locus_tag="VYO12_001063"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1587693.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQINVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARDGEKLVTLDGEERDLAETDLVITVADKPVALAGVMGGQATEISEKS
                     SRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVIQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIVSLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4457..5458)
                     /locus_tag="VYO12_001064"
     CDS             complement(4457..5458)
                     /locus_tag="VYO12_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1587694.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5614..6029
                     /locus_tag="VYO12_001065"
                     /pseudo
     CDS             5614..6029
                     /locus_tag="VYO12_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6155..6421
                     /locus_tag="VYO12_001066"
     CDS             6155..6421
                     /locus_tag="VYO12_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1587695.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            6682..8931
                     /gene="metE"
                     /locus_tag="VYO12_001067"
     CDS             6682..8931
                     /gene="metE"
                     /locus_tag="VYO12_001067"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1587696.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEANRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8995..9861
                     /gene="metF"
                     /locus_tag="VYO12_001068"
     CDS             8995..9861
                     /gene="metF"
                     /locus_tag="VYO12_001068"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1587697.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10420..12633
                     /gene="pnp"
                     /locus_tag="VYO12_001069"
     CDS             10420..12633
                     /gene="pnp"
                     /locus_tag="VYO12_001069"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1587698.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDYKPKKEFTETPKDSE"
     gene            12649..13266
                     /gene="cysE"
                     /locus_tag="VYO12_001070"
     CDS             12649..13266
                     /gene="cysE"
                     /locus_tag="VYO12_001070"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1587699.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13278..14162
                     /locus_tag="VYO12_001071"
     CDS             13278..14162
                     /locus_tag="VYO12_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1587700.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14244..15587
                     /locus_tag="VYO12_001072"
     CDS             14244..15587
                     /locus_tag="VYO12_001072"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1587701.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLAAQGIKLLDTKDGVRWTRD"
     gene            15580..15966
                     /locus_tag="VYO12_001073"
     CDS             15580..15966
                     /locus_tag="VYO12_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1587702.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            15970..16854
                     /locus_tag="VYO12_001074"
     CDS             15970..16854
                     /locus_tag="VYO12_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1587703.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            16991..17446
                     /locus_tag="VYO12_001075"
     CDS             16991..17446
                     /locus_tag="VYO12_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587704.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            17443..17589
                     /locus_tag="VYO12_001076"
     CDS             17443..17589
                     /locus_tag="VYO12_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587705.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            17732..17902
                     /locus_tag="VYO12_001077"
                     /pseudo
     CDS             17732..17902
                     /locus_tag="VYO12_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(18393..18473)
                     /locus_tag="VYO12_001078"
                     /pseudo
     CDS             complement(18393..18473)
                     /locus_tag="VYO12_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            18481..18843
                     /locus_tag="VYO12_001079"
     CDS             18481..18843
                     /locus_tag="VYO12_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1587706.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT         5770 a         3624 c         4271 g         5387 t
ORIGIN      
        1 ttacaaaaat tataaatagt taatttagat aaattgttta tataaattta gataagaata
       61 agctaacaag cagaagtaag ataggtcttg caagaatggt aaaaatctga taaactaagt
      121 agtaattgaa taggaatctg aaagactagt agcccaaggg aagtatatca gggaggagag
      181 ccgtgactgc aagctctcta tatgaaagct gggtgaattc acttgcgcat ggatttagaa
      241 atgaaggtct gacttgtcag atgaaaacgg atggtaccgc gtgtcaacgc tccgagtgga
      301 gtttttggca tgtggttttc tttttatcta cgagagactg atggaggaaa tatgtcaact
      361 attgaagaac aattaaaagc gcttcgcgaa gaaacgctgg ctagcttgaa gcagattact
      421 gctggaaatg aaaaagagat gcaagatttg cgtgtctctg tccttggtaa aaagggttcg
      481 ctcactgaaa tcctcaaagg gatgaaagat gtttctgctg agatgcgtcc aatcatcggg
      541 aaacacgtca atgaagctcg tgatgtcttg actgctgctt ttgaagaaac agctaagctc
      601 ttggaagaaa agaaagtcgc ggctcaactg gctagcgaga gtatagatgt gacgcttcca
      661 ggtcgtccag ttgcgactgg tcaccgtcac gttttgacac aaaccagtga agaaatcgaa
      721 gatatcttca tcggtatggg ttatcaagtc gtggatggtt ttgaagtgga gcaagactac
      781 tataactttg aacgtatgaa ccttccaaaa gaccacccag ctcgtgatat gcaggatact
      841 ttctatatca ctgaagaaat cttgctccgt acccacacgt ctccagttca ggcgcgtgct
      901 atggatgccc atgatttttc taaaggtcct ttgaagatga tctcgccagg gcgtgtcttc
      961 cgtcgcgata cggacgatgc gacccacagt caccaattcc accaaatcga aggcttggta
     1021 gttgggaaaa atatctctat ggctgatctt caaggaacgc ttcagttgat tgtccaaaaa
     1081 atgtttggtg aagagcgtca aattcgtttg cgtccatctt acttcccatt cacagagcca
     1141 tctgttgagg tggatgtttc ttgcttcaag tgtggtggag aaggctgtaa tgtatgtaag
     1201 aaaacaggtt ggatcgaaat tatgggggcc ggtatggttc acccacgtgt ccttgaaatg
     1261 agtggtatcg atgcgactgt atactctggc tttgcctttg gtcttggaca agagcgtgta
     1321 gctatgctcc gttatggaat caacgatatc cgtggattct accaaggaga tgtccgcttc
     1381 tcagaacagt ttaaataatg attagaaaag tagaaatggc agatgttgag gtgttggcta
     1441 aaattgccaa acaaaccttt cgtgaaacct ttgcgtatga taatacggaa gagcagttac
     1501 aggaatactt tgaagaggct tatagtctga aaactttgtc aactgagttg ggaaatcctg
     1561 actctgaaac ctatttcatt atgcatgagg aggagatagc tggttttctc aaagtcaact
     1621 ggggaagtgc tcaaactgag agagaattag aggacgcttt tgaaattcaa cgcctctatg
     1681 tgctacaaaa attccaagga tttggactag gtaagcaact gtttgaattc gcacttgaac
     1741 ttgctacaaa aaatagtttt tcttgggctt ggctaggtgt ttgggagcat aatacaaaag
     1801 ctcaagcgtt ttataaccga tatggttttg aaaaatttag ccaacatcat tttatggttg
     1861 gtcaaaaagt agatacggat tggttactga gaaagaaatt aaggtaagaa gaggattctt
     1921 ctattgaaat gataggaaag gaaaagaact agagtgacaa ctgtcattct ggagaactaa
     1981 attatgcttg tatcttataa atggttaaaa gaattggtgg acattgatgt gccatcacaa
     2041 gagttggctg aaaaaatgtc aactacaggg atcgaggtag agggtgtcga atcaccagct
     2101 gctggtctct caaaaattgt cgtcggtgag gtcttgtctt gcgaagatgt gccagagact
     2161 cacctccatg tttgtcagat taacgttggc gaagaagaag agcgtcagat cgtttgtggt
     2221 gccccaaatg tgcgtgctgg gatcaaggtc atggtggctc ttccaggagc tcgtatcgct
     2281 gataactaca aaatcaaaaa aggaaaaatc cgtggtttgg agtcacttgg aatgatctgt
     2341 tcacttggtg aattgggaat ttctgactca gttgtgccta aggaattcgc agatggcatc
     2401 caaatcttgc ctgaagatgc cgtgccaggt gaggaagtct tttcttacct agacttggat
     2461 gatgaaatca tcgaactttc catcacacca aaccgtgcag atgccctttc tatgtgtgga
     2521 gtggctcacg aagtggcagc catctatgac aaggcagtca actttaaaaa atttactcta
     2581 acagaaacta atgaagctgc ggcagatgcc ctttctgtca gcattgagac agacaaggcg
     2641 ccttactatg cagctcgtat cttggacaat gtgactatcg caccaagtcc acaatggttg
     2701 caaaaccttc tcatgaacga aggcatccgt cccatcaata acgttgtaga cgtgacaaac
     2761 tacatcctgc tctactttgg tcaacctatg catgcttttg acttggacac atttgaaggg
     2821 actgacatcc gtgtgcgtga agcgcgtgat ggtgaaaaat tagtgaccct ggacggtgaa
     2881 gaacgagact tggctgagac agacctcgtg attacagttg ctgacaaacc agtagccctt
     2941 gccggtgtta tgggtggtca ggctacagaa atttctgaaa aatctagtcg tgttatcctt
     3001 gaagctgctg tttttaatgg caaatctatc cgtaagacaa gtggtcgcct gaaccttcgt
     3061 tctgagtcat cttctcgctt tgaaaaagga attaatgtgg caacagttaa tgaagccctt
     3121 gatgcggcag ctagcatgat tgcagagctt gcaggcgcga cggtgcgtaa gggtatcgtt
     3181 tcagcgggtg agcttgatac ctctgatgtg gaagtttctt caacccttgc tgatgttaac
     3241 cgtgtcctcg gaactgagct gtcttatgct gatgtagaag acgtcttccg tcgtcttggc
     3301 tttggtcttt ctggaaatgc agacagcttt acagtcagcg taccacgtcg tcgttgggat
     3361 atcacaatcg aagctgatct ctttgaagaa atcgctcgta tctatggata tgaccgcttg
     3421 ccaaccagcc ttccaaaaga cgatggtaca gctggtgaat tgactgtgat acaaaaactc
     3481 cgccgtcaag ttcgtaccat tgctgaagga gcaggtttga cagaaatcat cacctatgct
     3541 ctgacaactc ctgaaaaagc agttgagttc acagctcaac caagtaacct tactgaactc
     3601 atgtggccaa tgactgtgga tcgttcagtc ctccgtcaaa atatgatttc agggatcctt
     3661 gataccgttg cctacaacgt ggctcgtaag aataaaaact tggcccttta tgagattgga
     3721 aaagtctttg aacaaacagg taatccaaaa gaagaacttc caaatgagat caacagcttt
     3781 gcctttgcct tgacaggctt ggttgctgaa aaagatttcc aaacagcagc agttccagtt
     3841 gatttcttct atgctaaggg aatccttgaa gccctattta ctcgtttggg actccaagta
     3901 acctatacag caacatctga aatcgttagc cttcatccag gtcgtacagc cgtgatttca
     3961 ctcggtgacc aagttcttgg tttccttggc caagtgcatc cagtcactgc caaggcttac
     4021 gatattccag aaacgtatgt agctgagctt aacctttcag ccatcgaagg ggcgctccaa
     4081 cctgctgttc catttgtgga aatcaccaaa ttcccagcag tcagccgtga cgttgccctt
     4141 ctcctcaagg cagaagtgac tcaccaagaa gttgtagatg ctatccaagc tgccggcgtg
     4201 aaacgtttga cagatatcaa actctttgac gtcttctcag gtgaaaaact gggacttggt
     4261 atgaagtcaa tggcttatag cttgaccttc caaaatccag aagacagctt aacggacgaa
     4321 gaagtcgcac gctatatgga aaaaatccaa gcgtctctcg aagaaaaagt gaatgcagaa
     4381 gtgcgttaaa cgatagtagg atacaaattc cccctctagt ttatgaacat tagaggggtg
     4441 attttatata aaataattaa aatgtgattt ctgtaaaaat acatctatga tcagagtttt
     4501 gaaaacttac aatgtctaaa tctttaacat agtagtggtt tttaggcaat aaaatatgat
     4561 ctattgttgc attaaaaagt tttggacttt ggctattcca agttcctctt tcaaaaggtg
     4621 gcagtgcatt taatgcgtcc ctatgagagc ttatttttgc aagtgctcca tgacgcatag
     4681 ttgcattaaa atcacctgca ataatagcct ttggatattt tgaagccaat tgattatgaa
     4741 tgatgtttaa gtcttgcttc cagatttcca ttaaacctgg cagaggagga gcagtatgca
     4801 aggcaatgat atctggtata ttttgttttc tcgaatgtaa tacaattgtc ccgaaccgtg
     4861 ttgtatgaaa agttttagct tctgtataga aaccataact tttcttgaca atcacagtca
     4921 caggagctat tccactattg gtaggtggag aagtgaaaat atcatagttg gccatagaaa
     4981 gtccaacttg atgaaacaat agtttgattc tctggttttc ttgctcacct ctgatattgg
     5041 tagctagttc agggaatata gccatatcgg cgtcaaaatg gctaaaaatt cgctcaatat
     5101 gttgtgcttc tatttgatta gcgacgttcc aagttactaa ttttaaggtt ttagtcttat
     5161 ttgatgcaga agaaagggtt ttatctgttc cgaataatag taatatgagc gatagtaaga
     5221 gcaaaatgcc agagtaaaac cgtatttgtt tatttttctt gtaaaaagat aggaaaaaaa
     5281 ataagaaact taaaatagca gccatagttg cagtagctaa ggggaaggca taaatctgtg
     5341 ccaagatagg ttgggtagat agccaaatgt taagattaga agatgggaaa aaagaataag
     5401 ttatgatagg gatagaaaag agaatgaccg aaagggcaga gagaaatgtt ttaatcattg
     5461 tgtgagtacc tcaatttgca atggttgctc ggagctaatg tgactttgtg actttacttg
     5521 attgatattc tttttcttgt cttcctcata gattaagtac aacccaagta aaaaaagtcc
     5581 aatttaagat tgaaatgatt aaaaggcaca gaaatgcaag acaattatac tacaaaaggc
     5641 aaacatttga caatcgatag ccgtcgctta atcgaacgat ggaaaaaaga aggaaaatca
     5701 aatagagaaa ttgcctctct acttggaaaa gctcctcaaa ctatccacac tgaaatcaag
     5761 cgtgggacag tccgacaatg tcttggaaaa gggcgcttca aagagattta ttctgccgac
     5821 tacgctcaac agtcttaaga aactaaagga aaagattctc cactatacat aaccaaaaac
     5881 ataaccaaaa attttcgcct gaaatgatgg tgatggctaa aggggttaac gtgggaattt
     5941 caaccattta ctattggatt catcgtggaa aattggggtt aagcaagcag gaactgcttt
     6001 atcctagaaa aggaaaatcg cttaagaaat aggctagcac caactttaaa cccactggtc
     6061 aatctatcga atcataattc gctcattttt ttaaaaatac atagacgtaa aaaaaagagg
     6121 gttaagtgat gaagaatagt gctattggga gtaattggaa ggatgtccat ctgagctctt
     6181 tagcaaggag gaaatccttg aaagtgatat gcgagtggct atcatgagcg agttgattga
     6241 ggctaggtat gaacaaggaa tcagtcagaa aaagctggaa gaagtcagtg gaataagcca
     6301 gcctgtcata gctaggatgg agacaggaaa gactagtcct cagttggata cagtcttaaa
     6361 agtcctagcc agtttaggaa agacactagc aatcgtccca cttgaacagg gaaaaagttg
     6421 actagaatca gttatagtct tttctaataa caggtcatag tcacttataa gaattactaa
     6481 taacatgttt tagtattcta attggaatat agctgtaaag taagaattac aaaggatttg
     6541 agacgtttgc ttcaagtctt tttcttttgt ccgaaacaga tatatagttt caaactatat
     6601 caaaccctaa ttttttacaa ttatacagac gctttctttt ctgttaagat agtttcaaca
     6661 acaaattttg gaggacacat catgtcaact acaatcatcg gtttccctcg tttgggcgaa
     6721 ttccgtgaat taaaatttac aactgaaaaa tactttagaa aagaaatctc agaagaagaa
     6781 cttttagcag cagcaaaaga cttgcgtgct aaacactgga acattgtcaa agaaaaaggc
     6841 atcactgaaa ttccatcaaa tgacttttct cactatgata acttcctaga tgcggctttc
     6901 cttttcaacg tggtgcctgc atccgttcaa aacttggact tgtctgacct tgagcgctac
     6961 ttcgctttgg gacgtggtta ccagggagaa aaaggggacg ttcgcgccct tccaatgaag
     7021 aaatggttca acactaacta ccactacatc gttcctaaat ttgaaaaaga cactcaagta
     7081 aaattggctg gtcacaagat tttcgatgag ttccaagaag ccaaagaact tggattgaac
     7141 actcgtcctg ttcttgtagg tcccttcact ttccttcaat tgtcagactt tgaagaaggc
     7201 gtgaaagcag acgacttcgt agatagctta gtggctgctt accaagaagt ttttgctaaa
     7261 ttggctgaac ttggtgcgac tcgcatccaa ttggatgaag cggctcttgt aaaagacttg
     7321 acagctgaag aaaaagctct cttcttgaac ctttacaaca aactcttggc tgacaaaaaa
     7381 ggtcttgaag tcttgcttca aacttatttc ggtgatgttc gtgacgtata cgctgacctt
     7441 gtaaacttac cagtagatgc tatcggtctt gactttgttg aaggtaagaa aactcttgaa
     7501 ctcgttaaag gtggcttccc agctgacaag actctctatg taggtattgt caatggtaaa
     7561 aacatctggc gcaacaacta cgaaaagagc ttggctgttc ttgagcaaat tccagctgaa
     7621 aacatcgttt tgacaagctc ttgctcactt cttcatgtgc catttacaac tgctaatgaa
     7681 gaatttgaac cagcactctt gaaccacttt gcctttgcag ttgaaaaatt ggatgaaatc
     7741 cgtgacttgg atgctatccg aaatggtcaa ggttcagaag ctcttgcagc caacaaagaa
     7801 ctctttgcga ctgagcgtgt tggtgaaaat gctgaacttc gtgcgcgtat cgctggcttg
     7861 acagacgcag actacactcg tttgccagcc tttgcagaac gtgaagctat ccaagaagaa
     7921 gctttcaaac ttccagctct tccaacaaca acgattggtt cattccctca aacaaaagaa
     7981 gttcgtgcca aacgtttggc ttaccgtaaa ggtgaattgt ctcaaaaaga gtacgacgct
     8041 ttccttgctg aaacgattga cgaatggatc aagtggcaag aagatattga ctttgatgtc
     8101 cttgttcacg gtgaatttga gcgtaatgac atggttgagt acttcggtca aaacttgtca
     8161 ggatacctct tctctaaaaa tggatgggta caatcatacg gtatgcgtgg ggtaaaacca
     8221 ccaatcatct ggggtgatgt cactcgtctt aaccctatca ctgttaaatg gtctagctat
     8281 gcacaaagtc gtacaaacaa acctgttaaa ggtatgttga ctggacctgt taccatcctt
     8341 aactggtcat tcccacgtga agacatctct atcaaggatt caactctcca aatcgccctt
     8401 gctatcaagg atgaagtact tgaccttgaa gctgctggtg tgaaaatcat ccaaatcgac
     8461 gaggctgctc ttcgtgaaaa attgccactc cgccgtagcg actggtacga agactacctt
     8521 gactgggcta ttcctgcctt ccgcttggta cactcaacag tagcgccaga cacacaaatc
     8581 cacactcaca tgtgttactc agaatttaca gatatcatcc cagctatcga taacatggat
     8641 gcagacgtta tttcctttga agctaaccgt tcaaaccttg aaatcttgga cgaactcaaa
     8701 gcgaaaaact tccaaacaga agtgggacct ggggtttacg atatccactc acctcgtgta
     8761 ccaaatgaag gcgaaatcga caacacaatc gaagccatcc ttgctaaagt gccaagcaag
     8821 aaagtttgga tcaaccctga ctgtggtttg aaaacacgtg gtatcccaga aacaaaagaa
     8881 agcttgatcc gccttgttga agcagctaaa gctgcgcgtg agaaattgta agattggatt
     8941 tctctccata cagtggttgt tcaaaaagaa atcaactaga aaaggttatt acatatgtca
     9001 cgccaaacac cgtcactctc atttgaagtg ttccctccaa acccagccgt gggtaatgat
     9061 aacattattt cagccttgca ggatatgcag gagttggctc cccattttat cagtgtaact
     9121 gccagcaata ataaatttaa tatcaaggaa acgacggttc gtttggctga ctttatccaa
     9181 aatgatttgg cgattccgac cattgcccac ttgccagcta tttatctaac taaggacaag
     9241 gttgctgaaa ctattgctga cttggacaaa gttggggtgc agaaaatctt ggctcttcgt
     9301 ggggatatta ttccagacgt ggaaccacaa aaggatttcc gctatgcaac cgacttgatt
     9361 gagtttatca aggaacaaac ccctcacttt gatattattg gagcttgcta tccagaaggg
     9421 catccagatt cgccaaatca gatttcagat attcaaaatc ttaagaagaa agtggatgca
     9481 ggctgttcga gcctcgtaac tcagctcttc tttgacaatg agcgcttcta tgattttcag
     9541 gacaagtgta tcttggctgg gattgatgtt cccattcatg caggaattat gccaattctg
     9601 aatcgaaatc aggctctccg actcttgaag acttgtgaga atatccatct tccacgcaaa
     9661 tttaaagcca tcttagacaa gtatgagcat gaccctgagt cgctcagagc agcaggactt
     9721 gcctatgcag tggaccaaat cgtggacttg gtaactcagg atgttgccgg tgtgcatctc
     9781 tatacgatga ataatgctga tacagcaaaa tacatccatc aagcaaccca tgccttgttt
     9841 aatcaccagt ctctaggata ataaaagcaa accattcttc tcaggtgagg ggaatggttc
     9901 ctttttaatg gtaaagacct cactttttaa gaaaaatatg ataaaataga ttctgtacgt
     9961 acttgataca aagatgaagg tattaaataa tacaatgcat ttaaactgta agatttaacg
    10021 acacgagtga gtcaaatcga tgttatttga cgaaagagtt agtaaagcat ttagctaatc
    10081 gcccgatagc gattagcgtg aaaggtttag atactttaat attaaacctt tctaatgatg
    10141 tatcttgaaa actgaacacg cctacaactc tgtggaaaaa gataaatctg cctaggagca
    10201 gtcgctccgt cgttcgattt cctattttcc tttgagttgc ttgacggctt agcatcttga
    10261 gtaattgaac acggccgaaa agctgtgtaa aaaagataaa ctgtcttgtc ttcatcgaag
    10321 acttcgtcag tttcctattt ttactttgct tttgacgtcc ttggtatctt gatctttgta
    10381 ggcaaggcgt ataatttcat caatccaaag gggattaaaa tggcaaaaca agtgtttcaa
    10441 acgacttttg cgggtcgtga gttaattgta gagactggtc aggttgctaa gcaagcaaat
    10501 ggctctgttg tcgtacgtta cggtgagtca actgtcttga ctgctgccgt tatgtctaag
    10561 aaaatggcaa ctggggattt tttcccactc caagtcaact acgaagaaaa aatgtatgcg
    10621 gctgggaagt ttcctggtgg ctttatgaaa cgtgaaggac gtccttcaac agatgcgacc
    10681 ttgacagcgc gtttgattga ccgtccgatt cgtcctatgt ttgcggaagg tttccgtaat
    10741 gaagtccaag tcatcaatac agtgctttct tatgatgaaa atgcatctgc accaatggct
    10801 gctatgtttg gttcatcttt ggcactgtct atttcagata ttccatttga cggaccaatt
    10861 gctggggtac aagtgggata tgtagatggc caaatcatca tcaacccaag tcaagaacaa
    10921 gcagagcaat ctcttcttga attgacagta gctggaacca agcacgctat caacatggta
    10981 gagtctggtg ccaaagaatt gtcagaagaa atcatgttgg aagcgcttct taaagggcac
    11041 gaagctgtca aagaattgat tgccttccaa gaagaaatcg ttgctgctgt cggtaaagaa
    11101 aaagcagaag tggaattgct tcacgtggat gctgaattgc aagctgaaat cattgcagcc
    11161 tacaacagtg acctccaaaa ggcagttcaa gtagaagaga aattggcccg tgaagctgca
    11221 actcaagcag tgaaagacca agtgactgcc gtttacgaag aaaaatatgc gaaccacgaa
    11281 gaatttgacc gtattatgcg tgatgtggct gaaatcttgg aacaaatgga acacgcagaa
    11341 gtgcgacgtt taattacaga agacaaggtg cgtcctgatg gtcgtaaggt cgatgaaatc
    11401 cgtcctttgg atgcggttgt tgacttcctt cctcgtgtac atggttcagg tctctttact
    11461 cgtgggcaaa ctcaagctct ttcagtcttg accttggctc cgatgggaga aactcaaatc
    11521 attgatggtt tggatccaga gtacaagaaa cgctttatgc accactataa cttccctcaa
    11581 tattctgtag gggaaacagg tcgttacggt gcgccaggtc gtcgtgaaat cggtcacggt
    11641 gcccttggtg agcgtgctct tgctcaagtc ttgccaagct tggaagaatt cccatacgct
    11701 atccgtctag tagcagaagt tttggaatca aacggttctt catctcaagc ttctatctgt
    11761 gcgggaactc ttgcccttat ggctggtggt gtgccaatca aggcgccagt agctggtatt
    11821 gctatgggac ttatctcaga tggaaataac tacacagtat tgacagatat ccaaggtttg
    11881 gaagatcact ttggagatat ggacttcaag gttgcaggta ctcgtgatgg gattacagcc
    11941 cttcaaatgg atatcaagat tcaagggatt actgcagaaa tcttgacgga ggctcttgct
    12001 caagccaaga aagcgcgttt tgaaatcctt gatgtcattg aagcaaccat tccagaagtt
    12061 cgtctagaat tggctccaac tgctccgaaa attgatacga tcaagattga tgtggacaag
    12121 attaagattg tcatcggtaa gggtggagaa accatcgaca agattatcgc tgaaacaggt
    12181 gttaagattg atatagacga agaaggaaat gtgtctatct actctagtga ccaagatgct
    12241 attaaccgtg ccaaagaaat tattgctggt ttggttcgtg aagccaaagt ggatgaagtt
    12301 taccgtgcta aagtcgttcg tatcgagaaa tttggtgcct ttgttaacct ctttgataag
    12361 acagatgccc ttgttcatat ctctgagatg gcttggactc gtaccaatcg tgtagaggat
    12421 ttggtagaaa tcggggatga agttgatgtt aaggttatca agattgatga aaaaggccgt
    12481 atcgatgcct ctatgaaggc tcttctacct cgtccgccaa aacctgagca tgatgaaaaa
    12541 ggtgaaaagt ctgagcgccc tcaccgccca cgtcatcaca aggattacaa acctaagaaa
    12601 gaatttacag aaacaccaaa agattcagaa taagaaaagg agaaatgtat ggggtggtgg
    12661 cgcgaaacca ttgatattgt aaaagaaaat gatccagcgg cccgcaccac tttggaggtt
    12721 ttgctgactt atccaggtgt caaggccttg gcggcccacc gtctctcgca ttttctctgg
    12781 aagcacggct tcaaactatt agctcgtatg tacagtcagt tttggcgctt ttggactcag
    12841 attgagattc atccaggagc ccagattgat tcaggtgttt ttattgacca tggttctggt
    12901 ctggtgattg gagagacagc gattgttgaa aaaggcgttc ttctctatca cggagtgact
    12961 ctcgggggaa cagggaaaga ctgtggcaaa cgccatccga ctgtacgaaa gggagccctc
    13021 atatcagccc atgcccaagt tatcgggcct gtggaaattg gtgaaaatgc caaggtcggt
    13081 gctgcagcag ttgtcgtagc agatgtacct agtgacgtga cggttgtcgg tattccggcc
    13141 aagattgtcc gtcttcatgg taagaaagat gagcctgtca ttcacgaagt cgaagaaaaa
    13201 cgagagtatt atgtcaataa actcgagcag gctaaagatg ccagtcacag atcgtctggt
    13261 ttgtagagga tacctatatg attcaagcaa gaaacaagtt aagccaagag gagctatctg
    13321 aggcgaaaaa agtaattaac tgttgccaaa actatgacgg tacctatcgc gatccctatc
    13381 tctctaacat gcttaatttt gacccaaaca tgcccgcctt tttcctttat tatgaaaaag
    13441 gcgaacttgt tggtttatta actgtctatg cagatgacca agatgtagaa gtgacgatac
    13501 tggttcatcc aggtcatcgc cgtcagggga ttgcgcgtgc attgtttact agttttgaga
    13561 gagaaacagc ttctttcccc attcggtcag tcacttttca gacagaacgt atttttttag
    13621 agaatcatcc tgactttgcc agtaactggg gactaatcga ggatgaagag acagaaactt
    13681 ggttaggtaa ggatagaaga ccttatccgt tagcaaatgt ttcccatctt gaaattttgt
    13741 tagcagatag ttcgtatcag gatcaaatta gtcaattaaa atttcaggca ttttcagagg
    13801 aacatgaatc gagagaagtt gtggatagat atgtcgctga agctctgaaa gatccagaaa
    13861 gccgactata tattttatta aaagctggtc aggttattgg aacttgtacg gttgatttat
    13921 cgactaatac gaattacctc tacggtttag caatattgga acctgaacgt ggaaaaggct
    13981 atggaagcta cttagcaaaa tccctcgtca accaactaat tgagcaaaat gacaaggaat
    14041 ttcagattgc agtggaagat agcaatgtag gtgccaaacg tttgtatgaa aaaattggct
    14101 ttgtcaaaca gactcaggtg gtttatctga atgagaaagg agcaagggat tccgaagtgt
    14161 agagatattc ggactgaaat ttacttgaac ttttagtgat gaaactaatt gttcttggat
    14221 ttcagctttc ctgattatga tttatgatta aaatctatga caccatgtct cgtgatttgc
    14281 gagaatttgt cccgattgag gacggcaaga tcaagatgta tgtttgtggg ccaacggtgt
    14341 acaactatat ccacgtggga aacgcccgtt cgacagtagc ttttgatacg attcgtcgct
    14401 attttgagta ccgtgggtat aaggttgcct atatttccaa ttttacagat gtggatgata
    14461 agattatcaa ccgtgccagg gaagaaggca tcacgcctca ggaggttgcg gataagtaca
    14521 tcgctgcctt tcgtgaggat gtgacggcct taggcgtgaa acctgcgact cgccatccgc
    14581 gtgtagtgga gtttatggca gacatcatcc gttttgtgga agacttgatc gagaaaggct
    14641 ttgcctatga gagtcaaggg gatgtctatt tccgtgtaga aaaatcccac aactatgcta
    14701 aattggctaa taaaaccttg gaagatttgg atctaggtgc ttcaggtcgt accgatgaag
    14761 aaacggctcg taaggaaaat cctgtagatt ttgctctatg gaaatcttcc aaaccaggtg
    14821 agatttcttg ggatagtcct tggggacctg gacgtccggg ttggcatatt gagtgttcag
    14881 tcatgtcgac agagatttta ggtgatacca ttgatatcca cggtggtgga gctgacctag
    14941 agtttccaca ccacaccaat gaaattgccc aatctgaagc taaaacaggc aaggcttttg
    15001 ctaactactg gatgcacaat ggctttgtca atatcgacaa tgtcaaaatg tctaagtcct
    15061 tgggtaactt cattacggta cacgatgccc ttaaaactct tgatgggcaa gtacttcgtt
    15121 tcttctttgc gacccagcac taccgtaagc ctatcaactt cacggaaaag gcagttcgcg
    15181 atgccgagac caatctcaag tatctgaaga atacttacga acaaccattt acaggaaacg
    15241 tggatgccca agagttacaa aactttaaag ataagtttgt agcagctatg gatgaagatt
    15301 ttaatgctgc caacggtatc acagttgtct ttgaaatggc caaatggatc aactcaggga
    15361 actatgatgc aagtgtcaag caagctcttg cagatatgtt agaaattttt ggaattgtct
    15421 ttgttgagga agttttggat gcagagattg aagacttgat tcaaaaacgc caagaggcgc
    15481 gtgccaatcg agactttgag acagcagacc aaatccgaga ccaactggct gctcaaggca
    15541 ttaaactatt ggatacaaaa gatggagtga ggtggacacg tgattgatgt caatctcatt
    15601 aacgggattg cgctagcctt tgagggggat gcggtgtatt ctatgtatat tcgccgtcac
    15661 ctcatcctca aaggtatgac caaacccaat aaactccatc aagaagcaac caagtatgtg
    15721 tcagccaagg ctcaggctcg cctgattgct ctcatgttgg aggagcaggt cctaacggaa
    15781 aaagaagaag aaatctataa acgtggccgc aataccaata gccacacaaa ggctaaaaat
    15841 gcagatgtcg tgacttatcg tatgtccacg ggatttgaag cggttatggg ctatctccat
    15901 atgactgaga atctggaacg tcttgagagt ttggtttcat ggtgcatcca aaaagtggag
    15961 ggctagaaca tgagggcaaa agaactacaa gactggtttc ctgaggctcg gatttcagac
    16021 caaccagtag agaaagaggg ctatctcacg ctccctttag cttctcagca gtggattttg
    16081 ctggaggaag ctgggctcag cgagcgtgaa aagcagttgg ttgccctttt gacccagcag
    16141 gagcaggctc gttcgctaaa cccttggtat tcctatctgg ttgaaggcaa gggacaggca
    16201 ccgcaagttt ttaaaaagat tcagttggtt tattgccatc tttcttattt tcagcaggaa
    16261 aatctggctt cttggctaga tatgatgcgg actctttttc cgaattgtca gacagtgcta
    16321 caggtcggag ctcaggatta tgttttcgtg cttcaacaag acaaatacac ttctgtaaga
    16381 gatattttaa gtgatacgat tgaagcggtt gagtatgact ttggacttcg tctttctatc
    16441 atgttgggtc aggtttggtc tcagacagga catcaagccc tatcagactt aatcaaagct
    16501 gagcgggatt tgttcaagac atggtggcgt cagggtcacc aaggtgttca tactttttct
    16561 cagctctatc tttggagtat gggagaaaga ctcgtggact tgaagccaat taaagaatgt
    16621 ctacaccaga tgattttgga tcaagatcag attcaggaaa tcattctctc tctttgggaa
    16681 aatagtgctg ttctcactaa aacagcccag caactctatc tgcaccgcaa ttctctccaa
    16741 tacaagattg ataaatggga agagttgaca gggcttcagt tgaaagagtt gaccgacctg
    16801 accttgtgtt atcaattgat tttaggttct ttgtcaacta tagttggttt gtaaagaagt
    16861 taatatttgg agaagaggat tgccatcttc tccattttta tgtgcagagt tatagtggct
    16921 tgatgctggg aaagtacact gtgactgcta aaacattcct agaagttgct ttgatttacc
    16981 taatctattt atgcaaattt tatgttattt tactataaca gttgttgcta agccaaataa
    17041 tagtggggaa gttcatttag acgtaagcat tgaagataat cagggaggta gtgggcataa
    17101 tttcagttct gtttcaagta gctcacaaac agctaaatat gaagaaactg gttataataa
    17161 caattcatca ttatatataa cgattgataa aacgtctgat gcaacagctc ttttgaaatt
    17221 aaagttgaat aatgttgata atcaacctgc tactgaagtt cctagttcag gaattactgt
    17281 aaaattaaat gctaaagata atgctggaaa ctggacaagt gcttcgaata aaaaagaaat
    17341 aacagtaaaa attgtttctg ctaaaccgac atatccagac aaaatcttag tgaaaaatcc
    17401 tgataatata aaagatacag aaaaaatgcc attattgaaa aattgaaaga ggcaaataaa
    17461 aatcatccag caggagctcc aacctttgct aaaggtgaag gagagcatgc aaatgatatt
    17521 gtagcaactt attcagatgg tacaacttat tatgtaccgt taaatgatgt gacaaaatat
    17581 gcgaggtagt ggctgtacca ctcacttatt cacctccccg tgatttgtag tagtgatagg
    17641 ctttctcact attattataa aacaaaataa agatcacaac actttttcat tctgtgttgt
    17701 gccttgagtg aaacgaaagg aatgaattat aaatatgaaa agtatagtca ctagcataga
    17761 tgagcgcttg cgtactcgcc tacgagtgat tatctggaag caatggaaga agaaatcgag
    17821 acgattatgg ggattgctta agttaggggt tcctaaatgg atagcagata aggtatctgg
    17881 ctggggcgac cactatcaat tatagtaaaa tgaaataaga ataggacgaa tcgttcagga
    17941 cagtcaaatc gatttctaac aatattttag aagtagaggt gtactattct agtttcaatc
    18001 tactatagta gctcagaagt cggtacttaa acgtgctata tcaaaaccag tccttgaaaa
    18061 acgtggactg gtttcgtgtt tggattatta ccttgaacga catgcgttaa aagttagttg
    18121 aaccgccgta tgccgaacgg cacgtacggt ggtgtgagag gggctagaga ttatccccta
    18181 ctcgatttcg aaatctagtg gaatgaatct ggaatagtcc atcgagcttt ctaatactct
    18241 ttgaaaatct cttcaaacca cgtcaacgtc gccttgccgt gtgtatggtt actgacttcg
    18301 tcagttctat ccacaacctc aaaacagtgt tttgagctga ctacgtcagt tctatccaca
    18361 acctcaaaac agtgttttga gctgactacg tcagttctat ccataacttc aaaacagtgt
    18421 tttgagcaac ctgcggctag tttcctagtt tgctctttgg ttttcattga gtataacaga
    18481 ttgttagaag ttggtttaaa tttcctaatc agtttgttca catttacctt cgatatatta
    18541 tatcccatag ttaaggttgg tcatacagat gattatagtc atggagccgt aaaacttagt
    18601 gtttctttag ttgacaaaga tgccatgaaa aaaatatctg taactgtaat aggatatttt
    18661 gaaataaata tagatgaaaa tatcaccgat attctatacg taaatggtac tgctattctt
    18721 tatcattatt tacgttctat tgtttcaata gtttcggcaa ttgatagcag tgaagcaatg
    18781 ttgctaccta ccattaatgt tttagagtta ctagataaat ctcaaccttt tgaagaagaa
    18841 taatttatta gctcactaaa ttgagggtaa ggaaaagtaa aatcagtaag aaaaatgtct
    18901 tgcattatac agcaaccttt tgggaatgag tagatggatt gaataaaatt ttattaagag
    18961 tggatgattt atctgtagat tattattgga cagttagtct tgaagtagtc taagaattag
    19021 gttataatca gtagaatcct tgctaataat ga
//
LOCUS       DAVLXF010000033        17565 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLXF010000033 DAVLXF010000000
VERSION     DAVLXF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.033"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(85..1026)
                     /locus_tag="VYO12_001080"
     CDS             complement(85..1026)
                     /locus_tag="VYO12_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1587707.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VYO12_001081"
     CDS             complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VYO12_001081"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1587708.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            complement(2952..3032)
                     /locus_tag="VYO12_001082"
                     /pseudo
     CDS             complement(2952..3032)
                     /locus_tag="VYO12_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3074..3304)
                     /locus_tag="VYO12_001083"
     CDS             complement(3074..3304)
                     /locus_tag="VYO12_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587709.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(3322..3963)
                     /locus_tag="VYO12_001084"
     CDS             complement(3322..3963)
                     /locus_tag="VYO12_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1587710.1"
                     /translation="MKLLKNLGWILLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(3975..4709)
                     /locus_tag="VYO12_001085"
     CDS             complement(3975..4709)
                     /locus_tag="VYO12_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1587711.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(4712..4909)
                     /locus_tag="VYO12_001086"
     CDS             complement(4712..4909)
                     /locus_tag="VYO12_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587712.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(4909..5115)
                     /locus_tag="VYO12_001087"
     CDS             complement(4909..5115)
                     /locus_tag="VYO12_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1587713.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(5125..5439)
                     /locus_tag="VYO12_001088"
     CDS             complement(5125..5439)
                     /locus_tag="VYO12_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587714.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(5706..5783)
                     /locus_tag="VYO12_001089"
                     /pseudo
     CDS             complement(5706..5783)
                     /locus_tag="VYO12_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5780..7069)
                     /locus_tag="VYO12_001090"
     CDS             complement(5780..7069)
                     /locus_tag="VYO12_001090"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1587715.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(7147..8394)
                     /locus_tag="VYO12_001091"
     CDS             complement(7147..8394)
                     /locus_tag="VYO12_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1587716.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNAYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLDLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(8585..9436)
                     /gene="rgg"
                     /locus_tag="VYO12_001092"
     CDS             complement(8585..9436)
                     /gene="rgg"
                     /locus_tag="VYO12_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1587717.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELLV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            9560..9901
                     /locus_tag="VYO12_001093"
     CDS             9560..9901
                     /locus_tag="VYO12_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1587718.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIEDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            9959..10039
                     /locus_tag="VYO12_001094"
                     /pseudo
     CDS             9959..10039
                     /locus_tag="VYO12_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10155..11858)
                     /gene="ilvD"
                     /locus_tag="VYO12_001095"
     CDS             complement(10155..11858)
                     /gene="ilvD"
                     /locus_tag="VYO12_001095"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1587719.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIIDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(12091..13023)
                     /locus_tag="VYO12_001096"
     CDS             complement(12091..13023)
                     /locus_tag="VYO12_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1587720.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     NFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(13020..13877)
                     /locus_tag="VYO12_001097"
     CDS             complement(13020..13877)
                     /locus_tag="VYO12_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1587721.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(13881..15227)
                     /locus_tag="VYO12_001098"
     CDS             complement(13881..15227)
                     /locus_tag="VYO12_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1587722.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(15240..15524)
                     /locus_tag="VYO12_001099"
     CDS             complement(15240..15524)
                     /locus_tag="VYO12_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1587723.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(15528..17558)
                     /locus_tag="VYO12_001100"
     CDS             complement(15528..17558)
                     /locus_tag="VYO12_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1587724.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT         5559 a         3829 c         3065 g         5112 t
ORIGIN      
        1 agatagaact gacgaagtca gtaacataca tacggtaagg cgacgctgac gtggtttgaa
       61 gagattttcg aagagtatta atcactaatt atctatctca acaaatcttc ctagaatatg
      121 aacattttcc gagacagaga caaaggagct tggatccact tgtgtcataa tctgtttaaa
      181 ttcattaaac tctgcacgtg taatgacagt gattaaaact gcctttctct cgtgattata
      241 ggttccttct gcatcatgga tcatggttgc tccgcggtgc aattttttat ggattttttc
      301 aattaccttc tctggatgat ttgtcacaat catggcctgc atacgctttt gcttagtaaa
      361 gactgcgtct gtcacacggc tagagacaaa gatggtaatc atagaataaa gagcgtattt
      421 ccaaccaaag gtcaaacctg ctatcagcat gatagttcca tttaccaaga aagaaatact
      481 accaacattc ttacccgttt tcttgcgaat agtcaggctg acgatatccg tcccaccact
      541 ggagatattg tttcgaagag caaaaccaat ccccaaaccc ataacaacac ccccaaaaag
      601 ggcattgata atgggatcct ccgtcaaggt tgccacaggg acaaactgga taaagaagga
      661 actcatagat accgtgataa aggtaaagac ggtgaactta tggccaatct gataccaagc
      721 taagaccatc aaagggaagt taatggcgta gaagcttagc gaaatcggaa tatgaaaacc
      781 aaaccagtga ttactcaagg cagagataat ctgtgccaga cctgttgcac cactcgaata
      841 cacatgccct ggttggaaaa agaaattaac tgctactgct gataaaaaac catagaccag
      901 agaggccgaa atcttctcat catacttttc tcgagagata ctttgtaaga cacgtaaaat
      961 ttttatctga taagcaaagc ggcgcagata atagcgccac cgcttaattc gttttgcttg
     1021 tttcatcttc ttctacttgt aagctgagtt cctctagttg tttgagagcg actgttgatg
     1081 gagcttgtgt cattgggtca gttgccttgt tgttcttagg aaaggcaatg acttcacgga
     1141 tattttcttc tccagcaagc aacatgacaa aacggtcaag cccgatagcc aaaccaccgt
     1201 gtggtgggaa accatagtcc atggcttcaa gaaggaaacc aaactggtca ttggcttctt
     1261 cagctgagaa accaagagcc ttgaacatgc gttcttgaag gtctttttgg ttgatacgaa
     1321 ggctaccacc accaagctca taaccgttca agacgatatc gtaagcaatg gcacgaacct
     1381 tagccaaatc accttctaat tcgtgagcag tctcttcctg tggaagtgtg aatggatggt
     1441 gggcgctcat gtagcggcct tcttcttcag accattcaaa catcggccag tcaaccaccc
     1501 aaaggaagtt gaacttatca ttatcaatca agccaagctc tttagcaata cgtccacgaa
     1561 gggcacccag tgttgcatta gccacttcaa gcgtatccgc cacaaagaga accaagtcct
     1621 tatcttcaag agcaagcgct gttgtcaatt cttcttggat accagtcaag aacttggcaa
     1681 ctggtccgtt taattctcca tcaaccacct tgacccaagc aagacctttg gcaccatact
     1741 gtttggctac ttccgtcatc ttgtcgatgt ctttacgtga atagttgtcc gcagctcctt
     1801 tgaccacaat cgcttttaca gcaggtgctt ctgaaaagac tttaaagtct acacctttga
     1861 ccacttctgt caagtcctga agcaacatgt caaaacgagt atctggcttg tcagaaccgt
     1921 aaagagccat agcatcatcg tatttcatac gagggaacgg tagcgttact tcgatgcctt
     1981 ttgtttcctt catcacgcgc gcgatcaagc cttctgtaat atcttggatt tctttctcag
     2041 taaggaagga cgtttccaag tcgacctgag taaattcagg ctggcggtct ccacgcaagt
     2101 cctcgtcacg gaaacattta acgatttggt agtaacggtc aaaaccagca ttcatcaaga
     2161 gctgtttcgt gatttgtgga ctttgaggaa gagcgtaaaa atgcccctta ttaacacgag
     2221 acggcactaa ataatcacgc gccccttcag gcgttgactt agaaaggaat ggtgtctcca
     2281 cgtcgataaa ctccaactca tccaaatagt tgcggataga gtgggtcacc ttggcacgaa
     2341 gtttaagatt ttccaacatt tctggacgac gaaggtcaag gtaacggtaa cgcaaacgtg
     2401 tatcgtcatt tgcctcaatg ccatccttaa tctcaaatgg tgttgtctta gctgtgttaa
     2461 gcacaataag agctgtcacg tttaactcaa ccgcaccagt tggcaactta tcattggctt
     2521 gctcacgcgc agcgacctga ccagtcacct caataacaaa ttcgctacga aggctttcag
     2581 ctgttgccat aacctctgca gatacttttt cagggttgat aaccaactgc atgattcctt
     2641 cacggtcacg aagatcgata aagatcaaac caccaaggtc acgacgacgg ccaacccatc
     2701 ctttcaaggt tatttcttgt ccgatgtgtt cctcacgaac acgaccagca tacatactac
     2761 gtttcattat ttctctcctc ttttattctg ttactatttt accataaaag cgcaggctct
     2821 tcatgaaaat catcagaaaa gtttgccagt ctttaaaagt caggtgaaag ccctaaaaat
     2881 tagcgctaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt accgtaggta
     2941 tggttactga ctccgtcagt tctatccaca acctcaaaac agtgttttga gcaacctgcg
     3001 gctagcttcc tagtttgctc tttgattttc attgagtata atacaaaaat ccgatgaact
     3061 tcaccggact cttttatttt gaatttttgc ctgctttacg cttttcagcg atttcggctg
     3121 cctttcgagg caagacaatt tccgttatgt aagccgtccc aaaacgcagt acacctgcaa
     3181 taggagcaaa gacaactgct agatagttat agaagaaatc gcctttgaag gcataagcta
     3241 gcgctccaat gatgaaaaat agaacgactg cctgaatcac tgctaataaa attactcgtt
     3301 tcatgtgacc tcctgactct attatagcat gagaatcatc aaaaagccga ctaaattatt
     3361 caaagcgtga agagaaatac tgtagaccag accttttctg ctaatgtaag ccaaacccaa
     3421 actaaaacca aggctaaaat agacaaaaaa ttgttgcaca tcacctggaa aatgaatcaa
     3481 ggcaaataga agactagata ccagaagaaa aatcagggtt cgtttactat tgtcctgctt
     3541 aggaaagaga tagcgtgcta acatccctct aaaaacaatc tcttccgtca aaggagcaaa
     3601 aataaccaca gcaaagaatg agaaaagtgg ttgagacaag gtcaagtctg tcgctatttg
     3661 ctgatttact gaaggatcat ctggcaagaa gaattgaacg accagagata agaaccaaac
     3721 caaggcagga agccaaataa atcggttaaa gccgctcttc tcaatataaa caggagcttt
     3781 ctgataccat ttgtaaatgc cgtacacata tactccagcc aagaccacat agagtagagt
     3841 aacagcatag ggtgaagcgc ctaaagcaag cgacgcagtc gcgagcccct gaataaagcc
     3901 atagataaag aaaaaggata gaagggctag aagaatccag ccaaggtttt taagtaattt
     3961 catagataac tcctttattt gaaataacgt tttaccatag gtaactgcat cacattgata
     4021 taaacatgga tggctcctac aagcaagaaa gctagtaact gaatctctcc tgtcaagaaa
     4081 gaaatgataa taagaaaaat atataaggct ggtaagacat attggtgtaa ttggaataaa
     4141 attcgaaaac tctgttccaa attagcctga cgctcccctt catcataaga atttatatag
     4201 ttcaagacat cctttggtgt agcgaaaaat tccaaatcaa actgacgaac aatcgcaatg
     4261 gttttaaaaa gagatttttg agcgactaag aataccacaa agagtaagaa agaaaggaaa
     4321 aatgtttgag ggtttgtatg caatataatc acctcactta atgaaataaa aatagccaat
     4381 ggaatcgcta cacctgtaat attaaaagca atggttccaa actcaagatt ccgatacatt
     4441 tgcacataat aggtttcatt cagatcgtca tccatttcct cttgatacaa ggaatgaaat
     4501 tttctgcttt tctttaagaa attgaaagtc aaaaacatac taatgaaacc tatcagtaaa
     4561 caaatagctg atatccatgg catcaaggct tttacatcta aaataatttc gtgggattcg
     4621 acacgtgcct taaacatccc tacaaacatg cccaagaacc cccccaagac aatagacatc
     4681 aaaaataaca atctacgttt ctttttcata ttcattctcc tttttcactt gctagatttt
     4741 tggatttctt ttcaatccat tcagttactg ggatgagagc aaagtagacc caaacaaatt
     4801 ggtcgctttg atagggatta aaccagctta ggtccatccc aatcagtaga aatacgctga
     4861 ctaataaagc tatgaccact acataataaa tcactttata cttgttcatc actcgtcctc
     4921 ctccaaacga aataccgatt cgactgtttc gttgaaaatt tgagatattt tcagggcaat
     4981 gataatggat ggggtgtact catcccgttc tagtaggcta atggtctgtc tggaaacccc
     5041 tgccagtttg gctaggtcgg tttgattgag accatcgcga gctcgaagct cttttagacg
     5101 attttttagt tgcatgttac acacctactc tccgtcaaat tcaacggttt ggatatcttc
     5161 aatacgttgc aacttgaatt tttctttccc ctttttatca acactacgta gtttgaccca
     5221 ttcctcgtca acatccacaa cttcccagtt atctggccca atatacactc ccgttataat
     5281 tggttccttt ccaatcattt cttgcaataa tctcgacatt tctgcgtttc ctttctcttt
     5341 tcgctcaagt tttttgattt tattctctag tttcttgatt tttttagaat tattagaata
     5401 aaagaaaatc ataaatagta taaatactag tacccacatt ataactcctt tctgcttcct
     5461 atttcttaac ttgaattcat tgtaacacat ctttttcttt ttgacaatta tagttgtcaa
     5521 aaagtttatg atttttgtca ttttgcaaaa gaaaaaggtc aggagtaagt tcctgaccac
     5581 tttatctatc attaatactc ttctaaaatc tcttcaaacc acgtcagctt caccttgccg
     5641 taggtatagt tactgacttc gtcagtttca tctacaacct caaaaccatg ttttgagctg
     5701 acttcgtcag ttctatccac aacctcaaaa cagtgttttg agcaacctgc ggctagcttc
     5761 ctagtttgct ctttgatttt cattgagtat aaaatcctag tttttcaaag atttctgaga
     5821 agttttggct gattgtctca agtgacactt gcacttcttc tcgggtttgg ttgttcttga
     5881 ccgtcacttg tccgctttcg acttcgctct ctcctagggt gatgagggtc ttagccgcaa
     5941 agacatctgc tgacttgaac tgagctttga gcttacggtt gaggtaatca cgctctgctt
     6001 tgaaaccttg ttggcgaaga gcttggacta gttctagggc tttgacattt gctccatcac
     6061 ccaagactgc gatatagaca tctagggcgt tttcgatagg gagggccacg ccttgttttt
     6121 caaggatgag aagcaggcgc tctacaccaa gcccaaaacc aaatccagca gtttcagggc
     6181 ctccaaagta agcaaccaaa ccatcgtagc gaccacccgc acagactgtc aagtcattgc
     6241 cctcaatctc tgtgataaac tcgaaaatgg tgtggttgta gtagtccaga ccacgcacca
     6301 tattggtatc gatgatgtaa tctactccaa gattttccaa catctgacgc acagcatcaa
     6361 aatgagtttg gctttcttca tcaagaaagt ccaagataga cggcgcattc tctactgcca
     6421 ccttgtcttc tttttcctta gagtccaaga cacgaagagg attttcctcc aagcgacgtt
     6481 ggctatcctt agacaaggtc tccttgagcg gtgtcaaata gtcaatcaag gcttggcggt
     6541 aggctgcacg gctctcagga tttccaagag tgttgaggtg caatttgaca ccttgaatac
     6601 cgatttcctt caaaaaatgg gctgccataa cgattgtttc cacatcggta gctggattgc
     6661 tagagccaaa acactcaaca ccaatctggt ggaattggcg caagcgccct gcctgtggac
     6721 gctcataacg gaacataggt cccatgtagt agaacttgct tggcttttgc acttctgggg
     6781 cgaagagttt attttccaca taggaacgga caacgggcgc agttccttct ggacggaggg
     6841 taatatggcg gtcacccttg tcatagaaat cgtacatttc cttggttacg atatccgttg
     6901 tatctccgac agagcggctg ataacctcgt aatgctcgaa aataggcgta cgcacttctg
     6961 cgtagttgta gcgcttgaaa atctcacggg caaagccctc aacgtactgc cacttagcag
     7021 actcagcagg taaaatatcc tgcgttcctt ttggtttttg taatttcata gggaatcctc
     7081 tttaaactta atagtcttat tttaccataa atagagggat taaaacagtg agaaaaaaat
     7141 taggatttag atatcatttt tgagattaag aattgtcaaa aaaatagcta gcaaggaaag
     7201 accaacaaat agcatccaag tcaactgtat attccatacg gctactagtg aaaaacaagc
     7261 tgttcccaca ggtatggata aggtaaacaa tagatctaaa aaattactag tacgagctag
     7321 aacctctgga gctagatttt tcatgagcat ggcactaatc tttggttgaa ctttaccaga
     7381 cacatacaga gtaaagaaga gaaatagcaa accaagcacg acttgattga ataaattagc
     7441 caaaccaact agactaagtc ctacagtcgc ccacatcatc aatctaggca aggactgctt
     7501 cccaaaataa gcattgcccg taaggctact gatgatgatt gctactaaaa cacagaattg
     7561 attgataaat agtgcctctg tataagaaaa attcaagaga gaatggctca aaaagaagat
     7621 attataaatt ccacccaaag cgccacccaa ggaattaata agcaagacag caaagagcat
     7681 aaaaccaaag tttttctgcc cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt
     7741 taggaactgg tctttgatag aaagcttctc attttttaag ttttcaccat cagcagatga
     7801 cattgacagg ctcaatttgc tttttcctaa aaagaggata gtggctgata ctaggaaaaa
     7861 gcaggcattg attcccgcaa cgagagaaaa attgttgacc gatagagcta agagccagac
     7921 tccgaaagct tgaccaccaa tagctgaaat ataggtgatg aactgtgaaa aagaataagc
     7981 ctccatcaga tcatcttcag ctactttttc cttaataaga ggcatacgca ggccacctgc
     8041 aaaatcactg atgatatcac taatgacatt gatcaaacac aggctagaaa aggcaaagag
     8101 actagcttgc tgaacaacta gggctgctag aaaaaataga accgcctgaa acaaaccgct
     8161 atagaccatc catttgacct tgtccctcgt gtaatctgcc cgaatccctg caaaaactgt
     8221 aaagaaggtc ggaagaatca tgacaatatt cgccatagca acagcaaaag atgcttgtga
     8281 caaggtcgat gcatagacga taaagaccag gttgaaaatc gaagcaccaa aagcattgaa
     8341 gaagcgtgat aaagtcaaaa ttctataagc tggatttcta aacaatagtt tcatagataa
     8401 tctccctcct tgttctaagt tctacatcaa gaatagcttt agtatagcac ttatgaattt
     8461 gcgaaacgat tcttttgaat atatgcaaca aacaaaatga gcaaaaaaac gatagaactt
     8521 ttgtaatgac ctgagagagg gtaacagttc tatcgcttca aattttttct tggtttgcag
     8581 atactcaaca ttcggtcgta tttctatagt attcggcaga tttattacag ccaagcatct
     8641 caaaaatacg gacagcatcc tccatctttt tctggccttc cttgactcta ccttgcttgc
     8701 tatcaaggag accttctgcc cacagataaa caattcggaa ataggtctca ttttccttgt
     8761 agaaatgctc ttcgataaca cgtttaaaat aataggcatt ggtaaattct tcacactcaa
     8821 tactagctaa aaagccattc aatagtatag tatgaaaaag gtttcgattg ccagacattt
     8881 ccattagaaa atcagattta cgtaccattt ctcgtacata tctagtaaaa agagaaacag
     8941 ataaaaatgg agaactgact gaaaataaat tgagttcata gattccccag atctcggtag
     9001 aaaacaaata atcatgaagg acttttcctt cctctgctgt taactctacc ctttcatcta
     9061 tgctcttcat ataagacttg ataataatgg catttagaat atgtttctgt ttgttgtgag
     9121 aatgggcatg cttttgatac tccctgcgat ataagtcctc aagaggtgct atattctttg
     9181 gttccaagac atctgtaatt tcttttctca actcagaatc tgtatcatac tggaaacctc
     9241 tcgccagaaa gaggatctcc tccacactgg cagatatatt ttccagagca aatagaaact
     9301 tttccaccaa aagctcactc tgacctgttt caaaacggga caacatagac ggcgaaaatt
     9361 gtcctccggt tgcttgtctc agtgagatat ttcttgactc tcgtaattgt ctaaagactt
     9421 ttccaatctg ctccatagac ttccccttga ttccgtattt tcttcatttt atcatatttt
     9481 tcagaaaatt catcaaaaac ttgccaaatt gtcagaatta tgagaaaata gaggatattt
     9541 atcacgtgga gggactgcta tgagagacga tatcaaaatc aatgaccgtg ctttggcctt
     9601 gcaagaccaa attatcgaaa aactagagaa agtttttgat acagatgtgg aattggatgt
     9661 ttacaatcta ggtctgattt atgaaatcaa tctggatgaa acggggctct gcaagattgt
     9721 catgaccttc accgatactg cctgtgattg cgccgaaagc ctgcctattg aaatcgtggc
     9781 aggtctgaaa caaatcgagg gtatcgaaga tatcaaggtt gaagttacct ggtcgcctgc
     9841 ttggaaaatc acacgaatca gtcgctatgg ccgtattgcc cttggactac cacctcgtta
     9901 agcaggccaa tcacttttaa agatgaaaaa agcaagccga aaatggcttg ctttttgata
     9961 ctcaatgaaa atcaaagggc aaactagaaa actagccgca ggttgctcaa aacactgttt
    10021 tgaagttatg gatagaactg acgaagtcag ctcaaaatac tgttttgagg ttgtggatag
    10081 aactgacgaa gtcagtaacc atacctacgg caaggcgacg ttgacgtgat ttgaagagat
    10141 tttcgagtat gagtttattt tttacctgac ttgtccatat tccagaagtc tgtcacggct
    10201 ccgcgtgaag cagatgatac gatgtgggca tatttaccga ggacaccacg gctgtaaagt
    10261 ggtggcaagg ttgtttctgc cttgcgtttt tcaagttctt cttcggatac ggccatggaa
    10321 atttctttgg tatcttggtc aaccgtaacg atatcgccgg tacggagata ggcaattggt
    10381 ccaccatcct gagcttcagg agcgatatgt ccaacaacca gaccataagt accaccagag
    10441 aaacgtccgt ccgtcaagag ggccacctta tctccctgac ctttaccaac aatcattgaa
    10501 gaaagtgaca gcatctcagg cataccagga ccacctttag gtccaacaaa acgaacaacg
    10561 actacatcgc catcaatgat ttcatctgtc aggacggcct gaatcgcatc ttcttctgag
    10621 tcaaagacct tagctggccc aacgtgacga cgcactttaa cacctgatac cttggcaact
    10681 gcaccgtcag gagcaaggtt cccgttcaag atgataagcg gaccatccgc acgttttgga
    10741 ttttcaagtg gcatgataac tttttggcct ggagtcaagt ctgcaaagtc agccaagttt
    10801 tcagctacag tcttaccagt acatgtgatg cgatctccgt gaaggaaacc atttgccaac
    10861 aagtacttca taaccgcagg gacaccaccg acttcgtaga ggtcttggaa gacatactga
    10921 ccagatggtt tcaagtcggc caagtgaggc acacgttctt gaatcgtatt gaagtcctca
    10981 agtgacaagt caacatttgc ggcatgggca atggcgagca agtgaagagt ggcgtttgta
    11041 gaaccaccga gagccatcgt tacagtaata gcatcttcaa aggcttcacg agtcaagata
    11101 tctgatggtt tgagaccaag ttccaacatc ttaacaacag cacgtcctgc tgcttcgata
    11161 tcttctttct tatcagctga ttcagctggg tgagaggatg accctggcaa actcatccct
    11221 agaacttcga tagcagttgc catggtatta gcagtataca taccaccaca accaccaggg
    11281 ccagggcagg cattacattc aagacgtttc acgtcctcag ctgtcatgtc accgtggttc
    11341 cattttccga taccttcaaa gacagaaacc aagtcgatat ctttaccatc aagatttccc
    11401 ggtgcaatag ttccaccata ggcgaaaata gctgggatat ccatattagc aatagcaatc
    11461 atagatccag gcatgttctt gtcacagcca ccgatagcga cgaaggcatc cacgttgtga
    11521 ccactcatag ccgcctcgat ggagtccgcg atgatgtcac gagatgttag agagaaacgc
    11581 ataccaggcg ttcccatagc gatcccgtcc gctacggtaa tggttccaaa ctgtacaggc
    11641 caagcgcctg cagatttgac accttcttta gccagtttcc cgaaatcatg caagtgaatg
    11701 ttacatggtg tattttccgc ccaagtcgaa atcactccca caatcgatgt ttcaaagtcc
    11761 ttatctgtca taccagtcgc acgaagcata gcacggttag gtgatttaac catgctgtca
    11821 taaatgctac tgcggtgacg tttatctaat tcagtcatct tatccctccc atttcagttt
    11881 ttactattat agcacaattt aaaggcaatg aacagaataa aattcttgaa ttttcagaaa
    11941 attctatacg catgtgaaat atttaaaatt aaaaacaaca aagcggatta gtgcactttc
    12001 tgatgaccag aatatgcttt ttaatccgct ttctttaaat aacgtactgt aatttttaca
    12061 gaaattcttt cagataagtg tatttaacat ctatcttgca ttataaattt ctagaacctt
    12121 ctcttttata ttcgattcac tcaaaccata ctcattaaga agataatcca ttttccctac
    12181 ttgaccgaat ctttcttgaa cacccatccg atgaattttt gttattccat catcagagaa
    12241 taattcacat aaagcactgc caattccacc tatctgattg tggttttcta cagtaaatat
    12301 agtttttcca cttaacattg tttttatctg ttctggtatc ggtttgattc taaataaatc
    12361 tatcacacct actgaataac ctaatttaga cagttcatct gcaactcgaa tacttggagc
    12421 aaccattatg ccagaagcaa cgattacaag atcttcacca tgtcttaact caatgtagcc
    12481 cttagaaaaa tcttctccac cttgatatac agcctctgga gcttttctaa ttgttcgaat
    12541 atattttagt ccttttaagt ctaatgtctg attcaatatt tcacgaaatt ggatatcatc
    12601 agttgcttca aaaatgattg atttaggaat taaacgtaac aatccaattt cttcaaatgg
    12661 catatgtgtc ccaccattca tttctgcagt tactcctgca tctgatccaa tcacagtggc
    12721 atccaattgt gcgtatccaa gagaaataaa taattgatca aatactcttc gtgaagcaaa
    12781 aggaccaaat gtatgaagat aaggtctaaa cccctgaata gacaagcctg ctgcaaggcc
    12841 gaccatttct gcttccataa tcccaacatt cacataacgg tctccaaagt tcttttcaag
    12901 attattagta gccatcgaac ttgacaaatc ggcttctaag actactatat cagaataact
    12961 ttgattagcc tctaaaagga agtctctata tacatgccgt aattctttcg tacttctcat
    13021 cattctgttt cctccaattc ctgacttaat ctttctacaa ctgaagttaa catttgtttc
    13081 tcctctacag tagggcgaag atgatgattg gatttcattt cttccagctc ttgaacccct
    13141 tgacctttaa tagtatctaa tacaatgcac ttaggtgatg aattatttga ctgttttaat
    13201 tggacaatcc cttcataaat ttctctaata tctgaaccct tgaccctaat ggattcaaat
    13261 ccaaatgctg aaaatttttc tacgaaatca cctggattac aaatatcctt tgtaaaacca
    13321 tctaattgtt ttttgttatc atcaacaaat acaattaagt tggataactg ttgatgagaa
    13381 gcaaactgta tagcctccca acattgtccc tcatttaact caccatctcc aacaatagca
    13441 taagtataaa agggactctt tcttattctc tgaccatatg caagtccagt tgcaacacta
    13501 attccttgtc ctaaagagcc cgttgtcata tctatgcctg gcgttagatt tctatcagga
    13561 tgagacggta atttggttcc atttgtattt aaagaatata agaattcttt gtcaaagaaa
    13621 ccattcaaat agagtgtact gtatagagct ggtcctccat gaccttttga taatatgaaa
    13681 taatctctat ctcgtgctgc aaatatttct ggagtcattg gcattatttc accataaagc
    13741 actgctaaaa cttctacgat agacagactt cctccgtaat gtccgaatcc aagatgattc
    13801 aatgttctaa gagtatttaa tcggatgtta gtcgcaaatt ttcttaaccc atcttctcta
    13861 tttttactta aaatcatccc ttattcctcc tttgcagatg gctttttaat aaaggatact
    13921 ccaaacataa ctgctagaat aagaacaaga ccaatcacaa tacctgcttg tgagccaaat
    13981 tgatttaaca ttcctaaaat aattcctgat agaccaaaat ctgcatctga gaaagttgat
    14041 ccttggaaac caagtcctcc caaaactggc attaaaaaga ctggaagaaa actgattaaa
    14101 ataccttgta aaaatgctcc aatagtggcg ccacgaacac caccagatgc attcccaatg
    14161 acacctgcag tcgctccaca gaagaaatga ggcacaacac ctggtaagat aacaaccgtt
    14221 cctgaagcaa tcataattgc catacttact aaaccaccaa caaaactaga gataaatcca
    14281 attagaactg cattgggtgc ataagtataa acaatcggac aatccaaagc aggttttgaa
    14341 ttaggtacaa gacgctctga aataccttta aaggctggaa caatttcgcc caaaataagg
    14401 cgaacacctg ctaaaataac aaatacccct gctgcaaatt gacctgctaa ttgtaaagca
    14461 taaactagac cacttgtacc actactgatt tctttttcta tatattctga ccctgcaaag
    14521 atagctacaa taatgtaaat aactgccata gataaagtga tactaacagt actatcacgt
    14581 aaaaaagcta aactctttgg aaatttaatg tcctctgttg attttgattt gtcaccgata
    14641 aggctaccag taaaaccact caaccaatat cccaaagaac tgaaatgacc taaagccacc
    14701 ttgtcatttc cagttaattg aaccatatat ttttgcacaa atgctgggga aatactcata
    14761 ataataccga gtgctaatcc tcctagtaag atgagaggca agctagtaaa gccagcaact
    14821 gataaaatga ccgcaatcat acatgccata tatagagtgt ggtgccctgt taaaaaaata
    14881 tatttaaatc gagtaaaacg agcgattaag atattgaaca ccatgcctgc aaacataatc
    14941 attgcagtag ctgagccata tgttgttaaa gctacagcta caattgcttc attattcggc
    15001 acaacgccag ataaatgaaa agcatgctca aacatggtac caaatggatt taaagaattt
    15061 tgtacaattc ctgcaccacc agatacaact aagaaaccaa caaaggtctt aattccacct
    15121 ttaataatat caggtaattt cttcttctga agaactaatc ctaaaattgc aattaaagct
    15181 actaaaatag ctggtgtact aacaatatcc aatatgaact tcatcatgac gctagcctcc
    15241 tatataagtc ctttttcttc acaaagttta gtaattaatt ctcgtagttc atccatatca
    15301 ataatactat ttaagatacg aacatctcca agatgactag ctgaatcagc tagatcacga
    15361 ccaacaatcc aaatatcagc tgcatttgga tctgctccac ctaaatcata atgttcaact
    15421 tctacatccg aaacattcaa atcactcaat acagattcaa tattcatctg taccataaaa
    15481 cttgaaccta atcctgaacc acaagctgta ccaattttta acattatcta atcctcctgt
    15541 ttaattatca ttttaatgtc atcatagttt tttgatgata ttaaagtttg aacatgattt
    15601 ttatctctta aaattgttgt taaatgtgac aaagccttta aatgactctc attatcaatg
    15661 gctgcaatac aaatcaacaa tcttacctct tgttctggat tatccaataa ataaatcggt
    15721 tcttccaaaa ctaacattga cattcctatt tcattcacac cttcatctgg ccgagcgtga
    15781 ggaattgcta ctcccttccc taaattaata aaaggtccaa actcttctac tttttgaatc
    15841 attgcctcag ggtagttctc agttatctta tcttgatcca aaagcggttt agctgctaaa
    15901 cgaatcgcct ccttccatcc taatttttgc gaactaacct gataggtttc tttggtaata
    15961 agttgttcta gcactggtac aatttccttt ctatcatttt tttggtaaag ataattcttt
    16021 aacgccaatc ttaattccaa ttcttgtgta ataattccat atcttttgac aatattcagg
    16081 atttgttcaa tctcaaaatc tccatactct aaattcggaa aatcttttaa cactagttct
    16141 actagttgta ttgcttgctc ttcagtcatc ataaccgaaa ctagataatt tggcttttct
    16201 gtctccacct ttatggtaga aaaaaccata tcatagtcac tactagcttt cacctgtaaa
    16261 tcatcaatct ttgaggttcc tataaactca atttgaggaa ataatgctaa tagattctct
    16321 tttaacatca atgaagaact aacaccatta ggacaaatga ttgctgcttt atagcatttt
    16381 tgaggcaaag tatctgcttt ctttaaataa cctccgaaat ggataacaaa atatgctgtt
    16441 tcactatcag gtatgggatt gtcaatagcg tccatcaagg gcatcaaaga atctttgact
    16501 aattcaaata aatcaggata atgttcttta acatgcaata catattcatt tgaactaggt
    16561 aggccgaact ttaatctata gtaagccggt ataaggtggc ggcgaagatt ttctctcaat
    16621 ccttcccttt gtttaaaatg taacaaagaa atatcttcca ttctacttat aatagcctct
    16681 gttaattgat taaagtaaac cggagcaaca tctacttcac cttcaaagca acttgataat
    16741 aaaactgtga tatagcgata atcatcctca gaaaacaccg tatctataat tcccaaatca
    16801 accactgtat ccaataaaat agtggttata tcttgaataa caggagatac taatgtctct
    16861 gaaagacata ctctttcaac atccctttga tacctacaca gaatgaatac taaaccgaaa
    16921 aggcaaactt ttaattgatt aacaataggt actagttgta gcttctcata ataatcttta
    16981 actacctgat ttatcaaatc ataagttaat gaataccccc aactggataa aacataatcc
    17041 aaaccccaaa tccctatgga ggattccagc aactcactaa ccatttgaaa agctaagcgg
    17101 tgcttattcc actctgaacc gtgtaaagta taaccttttg ctctactgta ccctagctcc
    17161 aaatcattat ctaacataat ctttcttaat gattgaatat cagataaggt tgtattctta
    17221 cttactttca aaaagtcttg gtaatgatta ttcgatataa aatctaatcg gcaaaaagtg
    17281 taaagataga ttaaagctaa gcgagtcgac tttggtaaaa ccaattcatc cgacttaata
    17341 atatctgtca aagactgctt cgtacgattt gataaactat agcgaccttg ttttttatcc
    17401 agcactatcc ctttattagc tagataaggc actaaataat ctattccttc tttgacttcc
    17461 tttataggta agctcacctt aacagataat tcatataatg atagctcaca atgatccatc
    17521 aaagtcatca aaataactag tgctctataa tcaaacattt ctctt
//
LOCUS       DAVLXF010000034        17459 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLXF010000034 DAVLXF010000000
VERSION     DAVLXF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.034"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(47..1384)
                     /locus_tag="VYO12_001101"
     CDS             complement(47..1384)
                     /locus_tag="VYO12_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1587725.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(1394..1660)
                     /locus_tag="VYO12_001102"
     CDS             complement(1394..1660)
                     /locus_tag="VYO12_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1587726.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(1925..2311)
                     /gene="rplQ"
                     /locus_tag="VYO12_001103"
     CDS             complement(1925..2311)
                     /gene="rplQ"
                     /locus_tag="VYO12_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1587727.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(2323..3258)
                     /locus_tag="VYO12_001104"
     CDS             complement(2323..3258)
                     /locus_tag="VYO12_001104"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1587728.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(3301..3684)
                     /gene="rpsK"
                     /locus_tag="VYO12_001105"
     CDS             complement(3301..3684)
                     /gene="rpsK"
                     /locus_tag="VYO12_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1587729.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(3702..4067)
                     /gene="rpsM"
                     /locus_tag="VYO12_001106"
     CDS             complement(3702..4067)
                     /gene="rpsM"
                     /locus_tag="VYO12_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1587730.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(4085..4201)
                     /gene="rpmJ"
                     /locus_tag="VYO12_001107"
     CDS             complement(4085..4201)
                     /gene="rpmJ"
                     /locus_tag="VYO12_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1587731.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(4226..4444)
                     /gene="infA"
                     /locus_tag="VYO12_001108"
     CDS             complement(4226..4444)
                     /gene="infA"
                     /locus_tag="VYO12_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1587732.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(4561..5199)
                     /locus_tag="VYO12_001109"
     CDS             complement(4561..5199)
                     /locus_tag="VYO12_001109"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1587733.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(5350..6660)
                     /gene="secY"
                     /locus_tag="VYO12_001110"
     CDS             complement(5350..6660)
                     /gene="secY"
                     /locus_tag="VYO12_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1587734.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(6673..7113)
                     /gene="rplO"
                     /locus_tag="VYO12_001111"
     CDS             complement(6673..7113)
                     /gene="rplO"
                     /locus_tag="VYO12_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1587735.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(7258..7482)
                     /gene="rpmD"
                     /locus_tag="VYO12_001112"
     CDS             complement(7258..7482)
                     /gene="rpmD"
                     /locus_tag="VYO12_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1587736.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(7454..7948)
                     /gene="rpsE"
                     /locus_tag="VYO12_001113"
     CDS             complement(7454..7948)
                     /gene="rpsE"
                     /locus_tag="VYO12_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1587737.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(7966..8322)
                     /gene="rplR"
                     /locus_tag="VYO12_001114"
     CDS             complement(7966..8322)
                     /gene="rplR"
                     /locus_tag="VYO12_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1587738.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(8406..8942)
                     /gene="rplF"
                     /locus_tag="VYO12_001115"
     CDS             complement(8406..8942)
                     /gene="rplF"
                     /locus_tag="VYO12_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1587739.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(9134..9532)
                     /gene="rpsH"
                     /locus_tag="VYO12_001116"
     CDS             complement(9134..9532)
                     /gene="rpsH"
                     /locus_tag="VYO12_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1587740.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(9817..10086)
                     /gene="rpsN"
                     /locus_tag="VYO12_001117"
     CDS             complement(9817..10086)
                     /gene="rpsN"
                     /locus_tag="VYO12_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1587741.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(10104..10646)
                     /gene="rplE"
                     /locus_tag="VYO12_001118"
     CDS             complement(10104..10646)
                     /gene="rplE"
                     /locus_tag="VYO12_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1587742.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(10670..10975)
                     /gene="rplX"
                     /locus_tag="VYO12_001119"
     CDS             complement(10670..10975)
                     /gene="rplX"
                     /locus_tag="VYO12_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1587743.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(11053..11421)
                     /gene="rplN"
                     /locus_tag="VYO12_001120"
     CDS             complement(11053..11421)
                     /gene="rplN"
                     /locus_tag="VYO12_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1587744.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(11447..11707)
                     /gene="rpsQ"
                     /locus_tag="VYO12_001121"
     CDS             complement(11447..11707)
                     /gene="rpsQ"
                     /locus_tag="VYO12_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1587745.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(11732..11938)
                     /locus_tag="VYO12_001122"
     CDS             complement(11732..11938)
                     /locus_tag="VYO12_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1587746.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(11948..12361)
                     /gene="rplP"
                     /locus_tag="VYO12_001123"
     CDS             complement(11948..12361)
                     /gene="rplP"
                     /locus_tag="VYO12_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1587747.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(12365..13018)
                     /gene="rpsC"
                     /locus_tag="VYO12_001124"
     CDS             complement(12365..13018)
                     /gene="rpsC"
                     /locus_tag="VYO12_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1587748.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(13031..13375)
                     /gene="rplV"
                     /locus_tag="VYO12_001125"
     CDS             complement(13031..13375)
                     /gene="rplV"
                     /locus_tag="VYO12_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1587749.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(13387..13668)
                     /gene="rpsS"
                     /locus_tag="VYO12_001126"
     CDS             complement(13387..13668)
                     /gene="rpsS"
                     /locus_tag="VYO12_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1587750.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(13772..14605)
                     /gene="rplB"
                     /locus_tag="VYO12_001127"
     CDS             complement(13772..14605)
                     /gene="rplB"
                     /locus_tag="VYO12_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1587751.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(14623..14919)
                     /locus_tag="VYO12_001128"
     CDS             complement(14623..14919)
                     /locus_tag="VYO12_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1587752.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(14919..15542)
                     /gene="rplD"
                     /locus_tag="VYO12_001129"
     CDS             complement(14919..15542)
                     /gene="rplD"
                     /locus_tag="VYO12_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1587753.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKLVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(15567..16193)
                     /gene="rplC"
                     /locus_tag="VYO12_001130"
     CDS             complement(15567..16193)
                     /gene="rplC"
                     /locus_tag="VYO12_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1587754.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(16410..16718)
                     /gene="rpsJ"
                     /locus_tag="VYO12_001131"
     CDS             complement(16410..16718)
                     /gene="rpsJ"
                     /locus_tag="VYO12_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1587755.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(16984..>17459)
                     /locus_tag="VYO12_001132"
     CDS             complement(16984..>17459)
                     /locus_tag="VYO12_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1587756.1"
                     /translation="NLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDIL
                     ALQAGMDVLTIEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELK
                     RRLARDTTVRNRYASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
BASE COUNT         4848 a         3849 c         3282 g         5480 t
ORIGIN      
        1 tatacacgtc taatcttaaa tagaacttaa aatttctcct attttcttaa tttttaaaac
       61 tatgaattgg tgctgggatt tgtccaccgc gagagatgaa gtcgacagac gaagccccat
      121 taaccttcat aacaggtgca gttcctaata gaccaccaaa ctcaatcata tcgccttctc
      181 ttcctttggg aatgatacga acagctgttg ttttcatgtt gataacaccg attgctgctt
      241 catccgcaat catagccgca atggtttcag caggcgtatc ttctgggatg gcaatcatat
      301 ccaatccaac agaacagata gccgtcatag cttctagttt ttctaaatta agagagccat
      361 tttgcactgc agcaatcatt ccttcatcct cagaaacagg gataaaggca ccagataaac
      421 caccgacttg gttgcaggcc atcactccac cctttttaac ttggtcgttc aagagggcca
      481 aggcagccgt cgttccatgc gtgccaactg tttctagccc catttcctca aggacacgtg
      541 ccacagagtc tccaaccgca ggggttggtg ccaaactcaa gtccacaata ccaaactcca
      601 cacccagtct ctcactggcc atttgaccaa ccaattgacc gatacgagtg attttaaagg
      661 cagttttctt aactgtttcg gctactacat caaagctctg tccacgaact ttttccaaag
      721 cacgtttcac aacaccagga ccagaaactc cgacattgat gataacatct gcttccccaa
      781 caccatgaaa ggcacccgcc ataaatggat tgtcctcaac agcattagcg aatacaacca
      841 acttggccac tcccatatct gaaagatttg ctgtttcctt gataattcgt cccatatctg
      901 ccacagccgt catattaata ccagacttgg ttgagccgat attgactgac gagcagacct
      961 tatccgtctc agccaaagcg cgaggaatgg aattgatgag aatctcatct cccttttgat
     1021 aacctttttg tactaaggca gaaaaaccac caataaagtc cacaccaatc tctttcgcag
     1081 ccttatcaag cgcttttgcc agaaccacgt agtccgtcgc atctgtcgct gccccaatca
     1141 gagaaatagg tgtcaccgat acacgcttat taacgatagg aattcccaac tcagccgcaa
     1201 tttcatcacc aacagctact aaattagccg cctttgtcgt aattttttga tagattttct
     1261 ccgcagcacg attgatattt ggatcgatac agtccaaaag agaaatcccc atggtaatgg
     1321 ttctaatatc gaagttttgc tcctcaatca tggcgatggt ttcagtaact tgtctaatat
     1381 ccatgatgac ctcctagata ttatacatag cttcgaaaat cgctgcactc tgaatattga
     1441 tttttacatt caaagtttgc ccaaaagctt caaattcatt acgaagatag gtaaaatctt
     1501 gcttttcatc actagataca acagccatca tcgtaaaata ttcatccaat acagtttgag
     1561 agatatcgtc aatattcaat cctaattctg caattttacc agaaacacct gcaacaattc
     1621 cagatttatc tttaccaaca acagttataa tcgctttcat aagtaaactc caattcttct
     1681 taaaataaga aaccttaaca ttaaacagga ttcttttcaa atagtatagc ataattctaa
     1741 cgaaaatact caaaaacgcc tgcttacgaa gttgttctta agaaaaaaac aatttcgtaa
     1801 acaagcgtct actatcccaa cttatgatga gtgttcccgc taggaccgaa atcctagcgg
     1861 tagaccagag ctagactaag agcacaagac tccatcatca taacactcaa caaaattgat
     1921 gattttatac taattcgatg atcgccattg gcgctgcatc accacgacgt ggttcagttt
     1981 taaggatacg agtgtatcca ccgttacgtt cagcataacg aggtgcgatt tctgagaaca
     2041 atttttgaag tgctgtagta gaagtgtact tatcagttgc ttcatcatag ttttcagatg
     2101 cgatttcatt acgtacgaaa gcagctgctt gacgacgtgc atgcaaatca ccacgtttac
     2161 ctagagtaat cattttttca acagttttac ggatttcttt agcacgagct tcagttgtca
     2221 cgattgattc gttgatcaaa aggtcagttg tcaaatcgcg aagcattgct ttacgttgtg
     2281 agctagtgcg tcctagttta cggtaagcca tgtattcctc ctttatttat cttttaatcc
     2341 aagacccaaa tcaatgagtt tgagtttcac ttcttccaaa ctcttgcgtc caagatttcg
     2401 tactttcatc atctctgctt cagatttttc tgtcaaatca tgcacagtat tgataccggc
     2461 acgttttaaa cagttgtatg aacgcacaga caagtccagt tcctcaatcg tacgatctaa
     2521 aatacggtcg tcagattcag tatcagcttc tttcatcact tcagttgact tagcaatctc
     2581 agtaagattt gtaaacaaat caagatgttc tgtcaaaata cgtgctgaaa gccctaaagc
     2641 atcttctgga ataattgttc catttgtcaa gatttcaagg gttaatttgt cgaaaccatc
     2701 attgctacct acacgagcag gttccacttg atagttgact tttgtaactg gtgtataaat
     2761 agaatctaca gcaagtgttc caactggtgc attatccttt ttattttcat cagcaggtac
     2821 atatccacga ccactgttaa cagtcatagt cgcttttaga gaagaacctt caccgattgt
     2881 aaagagataa tgatctggat ttacaatttc aatatcgcta tctgtcaaaa tgtcaccagc
     2941 tgttacttca gcaggacctt caacatccag ttcgatgatt ttttcgtctt caacgtacga
     3001 tttcactgca attcctttaa tgttcagaat gatttgcatc acgtcttcac gaacacctgg
     3061 aactgtgtca aactcatgta acacaccatc aatgttgata gatgtcacag ctgctcctgg
     3121 tagagaagct agaagtacac gacgaagaga gttaccaaga gttgtaccgt agccacgttc
     3181 aagtggttcg attacaaact tgccataatc tttattttca tcaatttttg ttatatttgg
     3241 tttttcaaac tcgatcattt agttactccc tcttaaacga aaagcagtgt aatgcgatga
     3301 ttatacacgg cgacgttttg gaggacgagc accattgtgt ggcactggag tcacatcacg
     3361 aattgctgtt acttcaagac cagcggcagc aagcgcacga atagctgact cacgaccaga
     3421 acctggacct tttacagtaa cttcaactga tttaagaccg tgttcttgtg cagatttagc
     3481 agcagcttca gaagccattt gagcagcgaa tggtgtagat ttacgagaac ctttgaaacc
     3541 aagagcacca gctgatgacc aagcaattgc attaccatgc acatcagtaa tcataacaat
     3601 agtgttatta aatgtagcgt gaatatgagc aataccagat tcgatattct ttttcacacg
     3661 acgtttacgt gttggtttag ccaagacttt tacctcctat attatttttt cttaccagca
     3721 atcgcaacag ctttaccttt acgagtgcga gcgttgtttt tagtattttg tccacggaca
     3781 ggaagtccac gacggtgacg gataccacgg tatgaaccga tttccatcaa acgtttgatg
     3841 ttcaagttta cttcacgacg aaggtcacct tcaactttga ttgcatccac ttcacgacgg
     3901 atagcatctt cttgatctga tgtaagatca cgtacacgaa catcttctga gattccagca
     3961 gcagccaaaa ttttcttaga tgttgcaagt ccgataccat aaacataagt caatgagatt
     4021 actacgcgtt tgtcatttgg aatatcaact ccagcaatac gagccatgtt ttctcctttc
     4081 tatcttatcc ttgacgttgt ttgtgttttg gatttgctgg gcaaattacc ataacacgac
     4141 cattacgacg aataacttta cagtattcgc aaattggttt gaccgatggt cttactttca
     4201 tttcttatcc ctccaagttt ttcgattatt taaagcggta agtgatacgt ccacgtgtca
     4261 agtcatatgg actcatttcg acagtaacac gatctcccgc taaaatacga atatagtttt
     4321 tacgaatttt accagaaact gttgctaaaa tctgatgtcc attttcaagt tcaaccgtaa
     4381 acattgcatt cggcattgta tcaactactt tgccttcaac ttcaatcaca tcgtcttttg
     4441 ccacgcaaaa gcacctccat aaatttcgat ttgatgcctc tagacacaga gacaacaatt
     4501 ataagtcaga ctatttcagt ataacatttg tagcggattt ttgcaagtgt gaaaaacgct
     4561 ttatttcaaa tttgtcaata ctttttcaat atctgagaag acatcattga tatcttgatt
     4621 accttcgatg tcatgaacca aacctttggc acggtagtga gcaatgattg gttctccttg
     4681 agcaatatta acatccaaac gacgttttac tgtctcaggc ttatcatctt cacgttggta
     4741 gtaatcttct tctttatagt caactggtgg gttaaagacc ttgtggaaag tttctccagt
     4801 tacgcggtgg atgatacggc cactcaaacg ttccaagagg ctgtcagggt tcacttcaat
     4861 attgataata ccttctagtt caatgccaag ttcagccaat gttttgtcca aggcatgagc
     4921 ttgttcaatt gtacgtgggt aaccatccaa taagaatcct gtttctttaa tatcatcttg
     4981 tgaaaggcgt tcttttacga ttccatttgt aacttcgtca ggaaccaatt cacccttgtc
     5041 aatatatgac ttagcaagaa cacccatttc agtttgattt gccattgcag cgcggaacat
     5101 atcacctgtt gagatatgtg caacatggaa ttgttctacg atttttgctg cttgagttcc
     5161 cttacctgca ccaggtaagc ccataatcaa aagattcatg atttgatctc cttattttat
     5221 ttttaaatag aagcaaaaag tcttttcacc cctatttaaa aacaaaatag aagaggggag
     5281 atccaactct tacaaatgtc agagtttaaa cctccactcc ctcagtattt actgattcag
     5341 taaatacttt tattctgttc tgtccatgaa accaacatac ttacgtttca ataggtaacc
     5401 ttccaattgc ttgattcctt cgatacctgt agagataatg atcaagagac ttgttccacc
     5461 aaaggcaaca acatcagaaa gaccaaatac atctttagct gcaatcggta aaatggaaat
     5521 cacaccaaga aagagggaac caacagttgc aagacgacga agaagtttag acatatattc
     5581 ttctgtacct ttaccaggac gaactccatg gatataggca ccactctttt gtaggctctc
     5641 tgctgctttt tcaggattaa tctgtacaaa cgtatagaag aatgtaaaga gaataatcaa
     5701 caaagcatac atggcaatac cagttggaga agtagttgcc aacatctctt gtgctaccct
     5761 tacccaagcc caatcatgac ctgtggcact caaaaactga agaatagccg caggcgctgc
     5821 agtaatcgaa ctggcaaaga taacagggat aactccagca gggtttacct ttaacggaag
     5881 gtaagagcta gatggagcac cttgtgcaac cttagtatat tggattggaa ttttgtattc
     5941 tgcttgttga acataagttg taaagtaaat aatcaacaat acagtaataa tcaaaatgat
     6001 tacgaaaatg atagatgaag tgatacggct acttgggacg ttcacaaagt agtccacata
     6061 gatgccctga atcatctctg gaattgagga aacaatcccg gcaaagataa tcatggaaac
     6121 accgtttccg tatcccttat ctgtaatttg ctcacccaac caagtgacaa tcatactacc
     6181 agctgttaag atgataccaa tcgtcagaaa aacttgtgga gttaaagcag ttttaatcaa
     6241 ttgagctcca gccaaggtat taaaaccagc tgtaatcccg atagattgca caaaagcgag
     6301 aactagagca atataacgag tagcttgatt caattttctt cgacctactt ccccttgttt
     6361 accccactct acaaacttgg gtaaaatatc catttgcaag agttggacaa caatagaagc
     6421 ggtgatatag ggactaactc ctagggcaaa aatcgaaaag ttttttaggg cattccccga
     6481 caccaagctc aacatgttta agaaggataa tccacttaaa gcattcaagc tattggcatt
     6541 cacaccagga actgtaatgc tagttccgat acgaaagacc aaaacgataa aaattgtaaa
     6601 taaaattttt gatcgaacct gcttgacttt aagagcttct cttaataatt taaaaaacat
     6661 aggtcacctc tcttagatga cttctactga accaccttta gcagtgatag cttcttcagc
     6721 tgatttagag aatttagctg ctttcacagt caatttctta gtcaactcac cgttaccaag
     6781 aattttaata cctgactttt cagctttaac aattcctgct tcgataagaa caactggagt
     6841 tacttcagca ccatcttcaa agacgttcaa ttggtcaagg ttcacaattg cgtattcttt
     6901 agcgttgatg ttagtgaatc cacgttttgg aagacgacgg aacaatggag tttgtccacc
     6961 ttcaaaacca aggcgaactc cgccaccgct acgagctttt tgaccttttt gaccacgacc
     7021 agatgtttta ccgttacctg atgaagtacc acgaccaacg cggttacgta ctttacgaga
     7081 accttctgca ggtttcaatt catgaagttt catttttatt ttctcctctt ttgtaaaatg
     7141 ctagcgccga taagggagaa aaggttgtct cccccatcaa ctcgcctata cgatgtcatc
     7201 atagatgact atatctagtt ttaggggatg gtacagtgca catcccctaa aacttcatta
     7261 gtttacttct tcaactgtta ctaagtgaga tactgctgta atcataccac ggatagcagc
     7321 gttatcttct ttaataacag agctgttcaa tttgccaagt ccaagtgcta caacagtttt
     7381 acgttgtgat ggaatgcgtc cgattggaga cttagtcaaa gtaattttaa tttgagccat
     7441 tttatcccct ttcttatgcc aaatcagaaa ctgaaatacc acgaagggca gcaatttctt
     7501 cagcgcgttt caattgtttc aaaccttcaa cagttgcacg aacaatgttg attggagtgt
     7561 tagaaccaag tgatttagat gtaatatctg ccacacctgc caattccaca acggcacgaa
     7621 ctgcaccacc agcggcaact ccagaacctt ctacagcagg tttcaacaat actttagctc
     7681 caccgaattc tgaaagaact tcgtgtggga ttgttgttcc aaccatagga acttcgatca
     7741 agtttttctt agcatcatct actgctttac ggattgcttc tggaacttct tgagctttac
     7801 cagtaccaaa tcctacgcga ccattgtggt caccaacaac aacaagagct gcgaaacgaa
     7861 gacgacgtcc acctttaaca acttttgtaa cacggttgac agcaactacg cgttcttcta
     7921 attcaactgc attgtcttta aatgccattt tctagtgtcc tcctattaga atttcaatcc
     7981 gttttcacga gctgcatcag ccaaagcttt cacacgtccg tgatatagat atccaccgcg
     8041 gtcgaacacc acttctgaaa tacctttagc gtttgcacgt tctgcaacga gtttaccgac
     8101 agcaacggct tgttcagttt tagttccttt tgaaacttct ttatcaagag ttgaagcact
     8161 tgcgagcgtt acacccgcta cgtcatcaat cacttgagcg tagatgcctg tattagaacg
     8221 gaatacgttc aaacgtgggc gatcagcagt tccagagagt tttccgcgaa cgcgacggtg
     8281 gcgtttttgg cggagtttgt ttttatctgg ttttgaaatc acagttttca cctctttagt
     8341 tttaaatcgt gtgctatgca caaagttgga aaataggttg gtggttgatg atcaaccact
     8401 caacattatt tacctgtttt accttcttta cggcgaacga attcaccaac gtaacggata
     8461 cctttacctt tatatggttc tggtgaacga aggctacgta cgtaagcagc tgtttgacca
     8521 actacttctt ttgaaattcc gctaacaacg attgttgttg ggtttggaag ttcaaaagta
     8581 attccttctg gagcttcaac ttcgtctgga tgagatttac caacagccaa aacaagttta
     8641 gatccttgaa gctgtgcacg gtaaccaacc ccacgcattt caagttcttt cttgaatcct
     8701 tctgatacac caacaaccat gttgttcaaa agggcacgag tagttccgtg gatagttttc
     8761 atttcttttg aatcgtttgg acggtgaaga gttatttcag taccttccac acggatttca
     8821 atatcttttg agaactcacg agtaagttct cctttaggtc cttttacagt tacaacgttg
     8881 tcattgttag cgagttcaac accagcaggc aacacgataa ctttattacc aatacgtgac
     8941 atgtttattt tctcctgtta aattgtcagg ccagaacggc cagttttcac ggggttcaga
     9001 tacttattta gttcaagagc tgaactgaac acgctctaag aactgtgcaa aatagataag
     9061 ttggcttgtt tgtaaacaaa cttctccaac ttcctaattt tgctttgttc tttacgagct
     9121 ttgtatcttg attttaccaa acgtaagcga taacctcacc accaacattc ttttggcgtg
     9181 cttctttatc agtaagcaaa ccttcagaag ttgaaaggat ggcaattcca agtccgttaa
     9241 gaacttttgg aaggtcttca cgttttttgt agacacgaag tcctggttta gaaacacgtt
     9301 tcaagttagt gataactttc tcaccatttg gtccgtattt aagaaataca cggatgacgc
     9361 cttgtttgtc atcttcaatg atttcaacgt tttttacaaa accttcgcgt ttaaggattt
     9421 cagcaatccc ttttttgatg tttgatgcag gtacttcaag tacttcgtgt ttagcttggt
     9481 tagcattacg aatacgagtt aggaagtctg cgattgggtc agtcataacc attttgtttt
     9541 tctcctctta ctagtagttt gcaagttgca cttgctagtt aatacatgat acaaagagcg
     9601 taacagcacg agcaaaaata gacaatttaa tgcagaagca atgctccaaa gcaaattagt
     9661 cttttttgcc aaagctgtag ctcgtgttca aattggcaag cccaattgaa cccgggctaa
     9721 actctgtgtg aaaaagataa actttcctag aaactttagt ttcttcgtca agtttcctat
     9781 ttttacttgg agttttgacg ctcttgatat cttaaattac caagatgctt ttgttacacc
     9841 aggaatttga cctttatgcg caagttcgcg aaaagcgata cgactcagac caaatttgcg
     9901 gtaaactgaa tgtgggcgcc ccgtaaccct acaacgatta tgtaaacgag tcggtgaggc
     9961 gttgcgaggt aatttagata aaccttcgta gtcccctgcc gcctttaatg cagcacgttt
    10021 ttcagcataa cggtcaacaa ttttttggcg tttagcctct ctagctacca ttgatttttt
    10081 agccattaga tttacctcct atattatttt gcaaaaggca ttccaaggcc tgtaagcaat
    10141 gcacgtgact cttcgtcagt gttagcagtt gttacgataa cgatgtcaag accacgagtt
    10201 ttgtcaacgt catcgaagtt gatttctggg aagattaatt gttctttcac accaagtgtg
    10261 tagttcccgc gtccatcaaa tgattttgtt gggacaccgt ggaagtcacg tacacgtgga
    10321 agtgaaactg ataccaattt atccaagaat tcgtacatac gttcaccacg aagggtaact
    10381 tttgcaccga tcgcaacacc ttcacgaaga cggaagccgg cgattgattt tttagcttta
    10441 gtgataagtg gtttttgacc tgagataagt gccaattctt cagcagcttt ttcaaggctt
    10501 ttagcgtttg atacagcttc accaacaccc atgttcaaaa caatcttatc tactttaggc
    10561 acagccatca ctgatgagta gttgaattgt tctgtcaaag caggaactac ttcattaaga
    10621 tatttttctt ttaaacgatt tgccattata cttctccttt ccttcgtgat taatcaagca
    10681 cttcgcctga ttttttgttg tagcgaactt ttttaccgtc tacaaatttg taaccaacac
    10741 gaccagctac accatttttg tccaaaactt gaacatttga tacgtggata gctgcttctt
    10801 tctcgatgat accaccttga ggaagctcgt tagttggacg ttggtgtttc ttaacaatgt
    10861 taacaccttc aacgataact ttgtttactt ttggaagggc agtaaggaca acagcttctg
    10921 ttcccttatc tttaccagcg attacgcgaa ctttgtcgcc tttttttaca aacattaggt
    10981 ttctccttga tttttcttac gcccataagg gcaccctagc ttgaagctag gggactagtt
    11041 tgttcctaaa aattaaagta cttctggagc aagtgacacg atcttcatga aaccaccttc
    11101 acgcaattca cgtgcaactg ggccaaagat acgtgttccg cgaggagttt tgtcttcacg
    11161 gatgataact gctgcgtttt cgtcaaattt gatgtatgaa ccatcagcac gacgagcacc
    11221 tgatttagta cgaacgataa ctgctttaac aacgtcacct tttttaaccg caccaccagg
    11281 agtagcttgt tttacagatg ccacgataac atcaccgatg tttgcaaatt tacgtcctga
    11341 accaccaaga actttgatag tcaagatttc gcgagcaccg ctgttgtctg cgactttcaa
    11401 acgagtttct gtttgaatca tttcagtttt ctcctttcag gtttgattag atgatgaccg
    11461 cttcttcaac aacttctaca agacggaaac gttttgtagc tgaaagcggg cgagtttcca
    11521 tgatacgtac gatatcgcct tctttggcaa cattgttttc atcatgagct ttgtattttt
    11581 tagagtagtt aatacgttta ccatagactg ggtggttacg ttttgtttca actacaactg
    11641 tgattgtctt gtccattttg tcagatacaa cacgtccaac aagaacttta cgattattgc
    11701 gttccattga aatttctcct tccctagtct attatttcgc ttcagattga actgttttga
    11761 tacgagcgat ttgtttttta acttctttca agcgagctgt ttgttccaat tgaccagtag
    11821 cagcttggaa acgaagttca aacaattctt ttttcaattc gttttcgcgc ttcgcgagtt
    11881 cttcttgaga aagaccacga agttctttaa caaattcttt tacttcatta agtttcatgc
    11941 cttctcctta ttctgcttca cgttttacga atttacattt aactggcaat ttgtggctag
    12001 caagtcgaag cgcttcacgt gcaatctctt cagatacacc agcgatttcg aacatcactt
    12061 taccacgttt aactggtgct acccaacctt caggtgcccc tttaccagat cccatacgca
    12121 caccgatagc tttagcagtg tatgatttgt gtgggaagat tttaatccaa actttaccac
    12181 cacgtttcat gtaacgagtc atggcgatac gagcagcttc gatttggcgg ttagtgatcc
    12241 agtggctagt tgtagcttga agaccgtatt caccgaatgc tacttctttt ccaccttttg
    12301 cttcaccgcg catttttcca cggaactcac gacggtgttt aacacgttta ggtactaaca
    12361 ttggttattt acctccttta gtgtttttac gagctggaag aacttcacca cggtagatcc
    12421 atactttaac accaagttta ccgtatgtag tatctgcttc ttcccaagcg taatcgatat
    12481 ctgcacgaag tgtgtgaagc ggaacagttc cttcagagta tccttcagca cgggcgatat
    12541 ctgcaccgtt caaacgacct gatacttgag ttttgattcc tttagctcca gcacgcattg
    12601 cacgttggat tgcttgtttt tgtgcacgac ggaaagcaac acgttgctcc aattgacgag
    12661 caattccttc acctacaagg tgagcatcca aatcaggttg tttgatttcg atgatgttga
    12721 tgtgtacttg ttttccagtc aatttgttaa gttttgcacg gagtgcatca acgttagcac
    12781 cacctttacc gataaccata cctggtttag cagtgtgaag tgaaacgtta actttgttta
    12841 ctgcgcgttc gatttcaata gttgaaactg ctgcgtcagc aagttctttt tgaacgaatt
    12901 tacggattgc aagatcttca tgaaggtaat ccgcgtattc tttttcagca taccatttgg
    12961 catcccaatc acggatgatg ccgacacgca taccaattgg atgtactttt tgacccacga
    13021 ttttacctcc ttatttttct gcaacagcta cagtgatgtg agctgtacgt ttgttgattg
    13081 gtgaagctga acctttcgca cgtggacgga aacgtttcat agttggtcct tcgtttgcga
    13141 atgcttcaga tactaccaag ttagctttat ccaaaccaaa gttgttttca gcgttagcta
    13201 cagctgagtt caaaactttc aagatgattt cagcagcttt gtttggagtg aatgtcaaga
    13261 ttgcgattgc atcggctacg cttttaccac ggatgttatc aagaacaaga cgtgatttac
    13321 gaggtgaaac acgtactgta cgagccattg ctttagctga agtaatttct gccatttatg
    13381 ttctccttat tttctacgtg ttttcttgtc gtctgcagcg tgacctttgt aagtacgagt
    13441 tggtgcaaat tcaccaagtt tgtggcctac catgtcttct tggatgtaaa caggtacgtg
    13501 tttacgtccg tcataaactg caatagtgta accaatgaaa cttgggaaga tcgttgaacg
    13561 acgtgaccaa gttttaataa cttttttctt ttcgtcgtta gcttgagctt caactttttt
    13621 catcaaatgc tcatcgacga aaggtccttt tttaagactg cgtcccattt ttatattttc
    13681 tcctttaaat gttgtaccac agcggcttgc actcctatgg agcgctaccg agctggcgga
    13741 tttactagtt gcttaagcga ctagtttaat attatttctc gttgcgacga cgaacgataa
    13801 gtttgtcaga tttcgctttc ttgttacgag ttttaagacc aagagcaggt ttgccccatg
    13861 gagtagatgg tgctttacga ccaactggtg ctttaccttc accaccaccg tgtgggtgat
    13921 cgttagggtt cattacagaa ccacgaactg ttgggcggat acctttccaa cggctacgtc
    13981 ctgctttacc aaggtttaca agtccatgtt gttcgtttcc gacaacacca actgtagcac
    14041 ggcaagttcc aagaatcata cgaacttcac ctgattgaag acgaacaaga acatatttac
    14101 cttcagaacc caatacttga gcagatgcac cagcagcacg taccaattca ccaccacgac
    14161 ctggtttcaa ctcgatgttg tgaatcaaag taccaactgg gatgttagca agtggaagag
    14221 cgtttccgac tttgatatct gcttctggac ctgaaacgat acgttgacct acttcaagac
    14281 cttttggagc gatgatgtat gctttcacac cgtcagtgta gtgtacaaga gcgatgtttg
    14341 cagaacggtt tggatcgtac tcgattgttt taacaactgc ttcaacgttg tctttattac
    14401 gtttgaagtc aaccaaacgg tagaaacgtt tgtgtccacc accttggtga cgaactgtga
    14461 tacgaccgtt gttgttacga ccagccttgc tcttcaatgc aacaagcaat gatttttcag
    14521 gagtgcttgt tgtgatttca gcgaaatcca aagaagtcat attacggcga ccgtttgttg
    14581 ttggtttata aacacgaatt cccacgatat ttcctcctta gattattcag cttcagcagc
    14641 aaacaactcg attgctttag aatcagctgt aagtgtgatg atagcttttt tagttttgtt
    14701 agtaaaacca gtgtaacgtc caacacgttt agcttttggt tttacgttga ttgtgttaac
    14761 attggcaact ttaacacctt cgaaagcagc ttcaacagct tgcttgatca aaagtttgtg
    14821 tgcacgagtg tcaacttcaa atacatattt ccctgcttca agttgagcca ttgagctttc
    14881 agtgatgaca ggttttttga taacatcata caaattcatt atgcaagaac ctcctcgatt
    14941 ttagagatag ctgcttgtgt gacaagaagt ttgtcgctat ttgcgatgtc aagaacactt
    15001 gcagttgtag cagttgcaac tttcacgttt ggaaggttac gagctgaaag agctgcgaat
    15061 tcatttcctt cttcaaggat aacaagaact ttagaatcga tgctcaatgc tgcaagaact
    15121 tttgcaaatt cagcagtttt tggagctgta aatgaaagag cgtctacagc tacgaatttg
    15181 ttttcagcaa ctttttcaga gtaaactaat ttaagagcta ggcgacgaac tttttgtgga
    15241 agtttgtagc cgtatgaacg tggagttggt ccgaagacaa caccaccacc acgccattgt
    15301 ggtgagcgga tagaaccttg acgagcacgt ccagttcctt tttgacgcca tggtttgcgt
    15361 ccaccacctg atactgcaga gcggttttta acagcgtgtg ttccttgacg aaggcttgcg
    15421 cgttggctga tgattacatc aaacacaact gattcatttg gttcgatacc aaatactgca
    15481 tcgttaagaa caacttggcc agcttcttta ccagtttggt caaataatgt tacgtttgcc
    15541 attgtgactg atttcccctt tctttattat ttaccagctt taactgctga tttgatagtg
    15601 ataagagatt tcttagcacc tggtacgtta cctttgataa ggataacgtt cttttctgga
    15661 acaacttgta caacttcaag gttttgaatt gttacgcggt cgccacccat acgtcctgca
    15721 aggtttttac ctttgaatac gcggttaggt gcaacaggcc ccatagaacc tggacgacgg
    15781 tggtaacgag aaccgtgagc cattggtcca cgtgattgtc cgtggcgttt gataacacct
    15841 tggaaacctt tacctttaga agtaccagtt acatcaacaa catctccagc tgcgaatgtt
    15901 tcaactgtga tttcagcgcc aacttccaag ccttcaacgt ttttgaattc acgaatgaag
    15961 cgcttaggag ccgtgttagc tttcgctaca tgtcctttag caggtttgtt gctcaatact
    16021 tcgcgtttgt catcgaaacc aacttggata gcgttgtatc cgtctgtttc aacagtttta
    16081 acttgaagaa caacgtttgg agttgcttca ataactgtta cagggatcaa ttcgccagct
    16141 tcagtgaaga tttgagtcat tcccactttt ttccctaaga ttccttttgt catgagaaaa
    16201 tagttccttt tctatatttt ttattcaaaa agtttttaac gagcgttttt tatgctcaag
    16261 tctaagatac aaagggcgtc aaactcaaag tgaaaatagg aaactaacgc agtgtctagc
    16321 ggacaatagg aagtttatct ttttcacacc gagtttagcc cgtgttcaat tagattgaaa
    16381 caccagcctc tgctctttta tattttagat taaagtttga tttctacgtt tacaccactt
    16441 ggaagatcca atttcatcaa ggcatcaact gttttttgag ttgggttaac gatatcgatc
    16501 aaacgtttgt gtgtacgcat ttcaaattgt tcgcgagagt ctttgtattt gtgagtcgca
    16561 cgaatgattg tgtagaggct acgttcagtt ggaagtggga ttggacccgc aacttgtgca
    16621 cctgtacgag tagctgattc tacgattttt gcagccgctg tgtcaagcgt acggtgttcg
    16681 taagctttca aacggatacg gatttttttg tttgccatct ttttctcctt ttcgtctatt
    16741 taagataata ggctagctcc acaagaaaac cgacgcggtg ttgcgtggca atgcaaccga
    16801 gcgtgtcgca acctcttgca tcaaaactaa ggctgtaatt tacagcacca taatagaata
    16861 acacaaatcc cctgcgattg caagggattt gttaggtttt ttcgtttttt tgggatgaaa
    16921 ctgttttcct actttttctt tctaaaatta gggattttct tctatatttc ttctatacat
    16981 aagttaagtc ctcttgacat caaatttatc ttctgattgg tccactaaga tatccgcctt
    17041 agactcagtt tctttatagt atcgcagata ctgctcccgt ctcatctgat ggctagccaa
    17101 tataaaggat gcatagcgat ttctcacagt cgtatctcta gcaaggcgtc gctttaattc
    17161 ggtctcctca tccgtgtaga aacagatggt tttttcaaag agttccttgg gtagaaaacc
    17221 aacagacatc ccttcgacaa tcaaaattgg tttggctcca gacaagacct cactagcctt
    17281 ccaaggttct tcaattgtca agacatccat acccgcctgc aaggcaagga tatctctctg
    17341 caaactctcc agttcatgcg ccactggcag actggctgtc accttttgat tcggcgcgtc
    17401 cttgggtact accaaatagc gtccggaggt gatataggga tctgtttcta gcaaattta
//
LOCUS       DAVLXF010000035        17021 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLXF010000035 DAVLXF010000000
VERSION     DAVLXF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.035"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            86..1111
                     /gene="trpS"
                     /locus_tag="VYO12_001133"
     CDS             86..1111
                     /gene="trpS"
                     /locus_tag="VYO12_001133"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1587757.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1263..2741
                     /gene="guaB"
                     /locus_tag="VYO12_001134"
     CDS             1263..2741
                     /gene="guaB"
                     /locus_tag="VYO12_001134"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1587758.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSINEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2792..3889)
                     /gene="recF"
                     /locus_tag="VYO12_001135"
     CDS             complement(2792..3889)
                     /gene="recF"
                     /locus_tag="VYO12_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1587759.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSKSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(3892..4260)
                     /gene="yaaA"
                     /locus_tag="VYO12_001136"
     CDS             complement(3892..4260)
                     /gene="yaaA"
                     /locus_tag="VYO12_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1587760.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4419..5669
                     /locus_tag="VYO12_001137"
     CDS             4419..5669
                     /locus_tag="VYO12_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1587761.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5666..6949
                     /locus_tag="VYO12_001138"
     CDS             5666..6949
                     /locus_tag="VYO12_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1587762.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            6991..7812
                     /locus_tag="VYO12_001139"
     CDS             6991..7812
                     /locus_tag="VYO12_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1587763.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7823..8368
                     /gene="pgsA"
                     /locus_tag="VYO12_001140"
     CDS             7823..8368
                     /gene="pgsA"
                     /locus_tag="VYO12_001140"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1587764.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8365..9192
                     /locus_tag="VYO12_001141"
     CDS             8365..9192
                     /locus_tag="VYO12_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1587765.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9177..10016
                     /locus_tag="VYO12_001142"
     CDS             9177..10016
                     /locus_tag="VYO12_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1587766.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10009..10803
                     /locus_tag="VYO12_001143"
     CDS             10009..10803
                     /locus_tag="VYO12_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1587767.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10862..11680
                     /gene="mreC"
                     /locus_tag="VYO12_001144"
     CDS             10862..11680
                     /gene="mreC"
                     /locus_tag="VYO12_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1587768.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11680..12174
                     /gene="mreD"
                     /locus_tag="VYO12_001145"
     CDS             11680..12174
                     /gene="mreD"
                     /locus_tag="VYO12_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1587769.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12268..13446
                     /locus_tag="VYO12_001146"
     CDS             12268..13446
                     /locus_tag="VYO12_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1587770.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13670..14449
                     /gene="rpsB"
                     /locus_tag="VYO12_001147"
     CDS             13670..14449
                     /gene="rpsB"
                     /locus_tag="VYO12_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1587771.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGHQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14528..15568
                     /locus_tag="VYO12_001148"
     CDS             14528..15568
                     /locus_tag="VYO12_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1587772.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEDGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15814..16137
                     /gene="tnpB"
                     /locus_tag="VYO12_001149"
                     /pseudo
     CDS             15814..16137
                     /gene="tnpB"
                     /locus_tag="VYO12_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(16278..16565)
                     /locus_tag="VYO12_001150"
                     /pseudo
     CDS             complement(16278..16565)
                     /locus_tag="VYO12_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16556..16954
                     /locus_tag="VYO12_001151"
     CDS             16556..16954
                     /locus_tag="VYO12_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1587773.1"
                     /translation="MKAPIPKAPLAHSFGSASIIAHTIHQKFNLKVPNYRQEEDWAKM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
BASE COUNT         5320 a         2734 c         3602 g         5365 t
ORIGIN      
        1 agcaaggctg atttattttg ataaattacg gttatttcat taaaaaaatg ctataattga
       61 aaggactata tcgaaggaga acaaaatgac taaacccatt attttaacag gagaccgtcc
      121 aacaggaaaa ttgcatattg gacattatgt tggaagtctc aaaaatcgag tattattaca
      181 ggaagaggat aagtatgata tgtttgtgtt cttggctgac caacaagcct tgacagatca
      241 tgccaaagat cctcaaacca ttgtagagtc tatcggaaat gtggctttgg attatcttgc
      301 agttggattg gatccaaata agtcaactat ttttattcaa agccagattc cagagttggc
      361 tgagttgtct atgtattata tgaatctagt ttcgttagca cgtttggagc gaaatccaac
      421 agtcaagaca gagatttctc agaaaggatt tggagaaagc attccgacag gattcttggt
      481 ctatccaatc gctcaagcag ctgatatcac agctttcaag gctaattatg ttcctgttgg
      541 gacagatcag aaaccaatga ttgagcaaac tcgtgaaatt gttcgttctt ttaacaatgc
      601 atataactgt gatgtcttgg tagagccgga aggtatttat ccagaaaatg agagagcagg
      661 gcgtttgcct ggtttagatg gaaatgctaa aatgtctaaa tcactaaata atggtattta
      721 tttagctgat gatgcggata ctttgcgtaa aaaagtaatg agtatgtata cagatccaga
      781 tcatatccgc gttgaggatc caggtaagat tgagggaaat atggttttcc attatctaga
      841 tgtttttggt cgtccagaag atgctcaaga aattgctgat atgaaagaac gttatcaacg
      901 aggtggtctt ggtgatgtga agaccaagcg ttatctactt gaaatattag aacgtgaact
      961 gggtcctatt cgtgagcgcc gtattgaatt tgctaaggat atgggagaag tttataatat
     1021 gattcaaaaa ggtagtgaaa gagcgcgtga agttgcgggt caaaccctat ctgaggtaaa
     1081 aggggcaatg ggacttcatt actttaatta agtttatttt acaagaaact atcatttcta
     1141 tctcgaagaa aagatagttt tttatttacc cctgtaacat ttgacaaatt tttatagaat
     1201 ggtatgatag atacaatata aaaagagtca agctcaaaaa gaaagaaaag aggaaacttc
     1261 gaatgtctaa ttgggacact aaatttttga aaaaaggttt tacctttgat gatgtattgc
     1321 ttattccagc tgaaagtcat gtgttgccta acgatgcaga tttaacaact aaattggcag
     1381 ataatttgac tttaaatatc ccaattatta ccgctgccat ggacacagtt acagagagtc
     1441 aaatggccat tgctattgct cgtgcaggtg gtctcggagt tatccataaa aacatgtcaa
     1501 ttgctcaaca agcagacgag gttcgtaagg taaaacgttc tgaaaatgga gttattattg
     1561 atccgttctt cttgacgcct gaacatacaa ttgctgaagc agatgagctt atgggtcgtt
     1621 accgcatcag tggtgttcca gttgttgaaa cacttgaaaa tcgtaaattg gttggtattt
     1681 tgacaaaccg agatcttcgt tttatttcag attataatca accaatttca aaccatatga
     1741 ctagtgaaaa tcttgttact gctcctgtgg gtacggatct tgcaacggct gagagtattc
     1801 ttcaagagca tcgtattgaa aaacttccgt tggtcgatga agaaggcagt ctttctggtt
     1861 tgatcactat caaagatatt gaaaaagtta ttgagtttcc aaatgcggct aaagatgaat
     1921 ttggtcgtct tctagttgca ggtgcagtag gtgttacttc agatacattt gaacgtgcag
     1981 aggctctttt tgaggcagga gcggatgcga ttgttattga tactgcacat ggtcattctg
     2041 caggtgtctt gcgtaaaatt gccgagattc gtgctcattt cccagatcgg actttgattg
     2101 ctggaaatat tgctactgct gaaggtgcac gtgcccttta tgaagcgggt gtagacgttg
     2161 ttaaggttgg tattggacca ggttctatct gtactactcg tgtgattgct ggtgttggtg
     2221 ttccgcaagt aacagctatc tacgatgctg cagctgttgc gcgcgaatat ggtaaaacga
     2281 ttattgctga cggtgggatc aagtattctg gagatattgt aaaagcactt gctgcaggtg
     2341 gaaatgctgt tatgcttgga tctatgtttg ctggaactga tgaagctcca ggcgaaactg
     2401 aaatcttcca aggacgtaaa ttcaagactt accgtggtat gggatcaatt gctgctatga
     2461 agaaaggttc aagcgaccgt tatttccaag gttctatcaa tgaagcaaac aagctcgttc
     2521 cagaaggaat tgaaggtcgt gttgcttata aaggagcggc agctgatatt gttttccaaa
     2581 tgattggtgg tattcgctct ggtatgggtt actgtggtgc agctaacctt aaagaactac
     2641 acgataatgc tcaatttatt gaaatgtctg gtgctggttt gaaagaaagc catcctcatg
     2701 atgtgcaaat tactaatgag gcaccaaatt attctatgta aaaaacaatg aaaagaactc
     2761 cagtgaaaac aggagttctt ttacaatgtt gtcaatttcc atttacagca gctttaccat
     2821 cctgaatagt gaagatactt agattttctg gtagattttg aagatggtct aagcttgttg
     2881 ttgtgataaa ggtttggatt gattgagaaa tcgtttctaa taattttaat tgtctagtgt
     2941 tgtcaagttc actcatcaca tcgtcaagca gtaatatagg agattctgtg gtaatgcttt
     3001 ccattaattc gatttctgct aattttatcg agaggacgag actacgatgt tgaccttggc
     3061 ttccgaaact agcatccatc ccatttatat aaaaagaaat gtcatctcga tgaggaccga
     3121 caccagtatt ctttttaaat aaatctctgg atttactttt ttctaaagca attttgaaag
     3181 attcggataa gttttgtttg tcagttatat tgacagaaga ttgataggat attgacaact
     3241 cttcgatctg attagagagt tcaaaatgtt tcttacgccc aaatgattct agttttttta
     3301 tgaaatctaa gcggtgattc attacacgac atccataatc aactagctga tcatctaaca
     3361 cagaaaggaa tgtttcatct attttttgat ctgattttag gtaagtgttt ctttgcttta
     3421 ggatgtggtt ataattggtt aagtcagata agtagattgg cttaatttgc ccaagttcca
     3481 tatcgatgaa ttttcgtcga atcgaaggtg ctcctttaat tagttgtaaa tcttcaggag
     3541 caaataagac aacattcatg tgtcctacat aatctgaaag gcgtgcctgt tttaagtgat
     3601 taacttttgt tacacggcct ttttgtgtta gttcgatttc taggggaatg gatccagttt
     3661 ttttctgaac gagacctgaa agatgaagtt gttcctcatc aaaatgaatg agatttttat
     3721 ctgttcgagt tcgatgacta cgcgttaagg ctaaaaaata gatagcctct aacatgtttg
     3781 tttttccttg tgcattacgt cccaaaaaga cattcaattt aggattaaag tctattttcg
     3841 tttctttgta attacgaaaa gtcttgagag atagatgttg tagccacatg attatctacc
     3901 agggaatcgt ggagcttgtt ggcttttagg tgatgaagca ggtttcgatt tgtctttttt
     3961 aactccctta ttcatctttt tgacaagttt agcgatccgt tctttttcaa ctttatcagc
     4021 ttggtattca tcttgttctt cagaagtagg ttgtgtcaac aagatgtcaa tgttcatgtc
     4081 agggatgtca actttatcgc caatacgaag tttttttcca cgacgactct ctaattccct
     4141 attaaagtaa acagaatgtt cagagagaaa tgatttaata gctcctccgc tatgtgtaat
     4201 tccaagttct ttgagtagtg cttggagggt aataaattct tcaaataatt tgtattccat
     4261 aattcacctc aatctttggt attataccat attttcctaa aaatagtgag agagacagcg
     4321 agaaatgtaa gcgattttta tttcaaaagt gttacaggga acaagaaaat ttcaggtttt
     4381 cgtgatataa tagaagtctg tatataagga ggtaaatcat ggagttagtg catggaattt
     4441 caacacattt tatccaatca aaaaagttta aaacaaacaa aattaccgtg cgttttaccg
     4501 ctccattatc ccttgatacg attgcaggtc acatgttgag tgcaagtatg ctagagactg
     4561 ctaatcagat gtaccccact tctcaagatt tgaggagaca cttggccagt ctatacggta
     4621 cagatatgtc aaccaattgt ttcagaagag ggcaaagcca cattatagaa ttgacattta
     4681 cctatgttcg tgatgagttt ttaagtagga aaaacgtgct aacctctcag attttggaac
     4741 ttgtaaaaga aactcttttt tcacccgtag tagttgataa tgggtttgat ccggccttat
     4801 ttgaaattga gaaaaaacaa ttgctagcaa gtttagcagc tgatatggat gattcttttt
     4861 attttgcaca taaagaattg gataaattgt tttttcatga tgaacgtctt caattggaat
     4921 atagtgattt acgaaatcgt attttagctg aaactccaca aagttcttac tcttgtttcc
     4981 aagaattttt agccaatgat cgaatagatt tctttttcct aggtgatttt aatgaggttg
     5041 aaattcaaaa tgtattagaa tcatttggct ttaaaggtcg aaaaggagat gtgaaggttc
     5101 agtattgtca accttattct aatatccttc aggaaggtat ggttcggaaa aatgtgggac
     5161 aatccatttt ggaattaggt tatcattacc gttctaaata tggtgatgaa caacatttac
     5221 ccatgattgt aatgaatggt ttacttggtg gatttgctca ctctaagctc tttacaaatg
     5281 tccgtgaaaa tgctggatta gcttatacca tttcaagtga gcttgattta tttagtggat
     5341 tcttgaggat gtatgctggt atcaatcgag aaaatcgtaa ccaggctcgt aaaatgatga
     5401 ataatcaact gcttgattta aaaaaaggtt attttacaga gtttgagtta aatcagacca
     5461 aggaaatgat tcgttggtcg ttgttacttt ctcaagataa tcaatcttca ttgattgaac
     5521 gtgcttatca aaatgcctta tttggaaaat cttcagcaga ctttaaaagt tggattgcaa
     5581 agcttgaaca aattgacaaa gatgctattt gtagagtagc taataatgtg aaactacaag
     5641 cgatttactt tatggaagga atagaatgac aaaggttgtt tttgaagaaa aatactatcc
     5701 agctgtaaaa gaaatggttt atcgaactcg tttggccaac ggattgacag ttgctctttt
     5761 gcctaaaaag gaatttaaag aggtttacgg gagtgtcact gtacagtttg gttcggtaga
     5821 tacgtttgtc acagaagttg acggagatgt aaaacaatat cctggaggaa ttgctcattt
     5881 tcttgaacat aaattatttg agagagaaga ttctagtgat ttgatgtcgg cttttacgag
     5941 tctaggtgca gatagtaatg cctttacaag ctttacaaaa acaaactatc ttttttcagc
     6001 aacggattat tttttagaaa atttagattt acttgatgaa ttggtaacat cagcacactt
     6061 tactgaagct tccattttga cagagcagga tattattcag caagaacgag aaatgtacca
     6121 agatgatcca gattcgtgtt tattcttttc aactttagcg aatttgtatc ctggtacacc
     6181 tttagcaact gatatagttg gaagtgagga gtccatttcc caaatcaatc taactaattt
     6241 gcaagaaaat tttacaaagt tttacaaacc tgtaaacatg tctctgtttt tagttggtaa
     6301 ttttgatgtg gagcgagtac aggactattt tgaaagcaaa gaactgaaag attcagattt
     6361 tcaggaagta gcaagagaaa agttgttttt acagcctgta aagccaacag atagtatgag
     6421 aatggaagta tcttctccca aactagcgat tggagttaga ggtaagcgag aagtttctga
     6481 agcggattgc tatcgacatc atattttatt aaaattattg tttgcaatga tgtttggttg
     6541 gacttcggat cgttttcaaa aatgttatga atcaggtaaa attgatgcat ccttatctct
     6601 ggaagttgaa gtaacaagtc gctttcattt tgtcatgttg acaatggata cgaaagagcc
     6661 agttgctttg tctcatcaat ttaggaaggc tattcgtaat tttacaaagg atttagatat
     6721 tacagaggaa catttagata ttatcaaaag agagatgttt ggcgaatttt tcagtagcat
     6781 gaactctctt gaatttattg caacgcaata tgatgctttt gaaaatggtg agacaatttt
     6841 tgatttgccg aaaattttac aggaaattac tttagaggat gtccttgatg ctggacatca
     6901 tttaatagat gatggtgaca tagttgattt tacaatattc ccatcgtagt aacctattat
     6961 aatagacact agaaagaagg gatgacaagt atgagaaaaa aaacaattgg agaggtttta
     7021 cgattagcta gaatcaatca gggattgagt ttagatgaat tgcagaaaaa gacagaaatc
     7081 cagttagata tgttggaagc aatggaagca gacgatttcg atcaacttcc aagtcctttt
     7141 tacacgcgtt ctttcttgaa aaaatatgca tgggctgttg agttagatga ccaaattgtt
     7201 ttggatgctt atgattctgg gagtatgatt acttatgagg aagtagatgt tgatgaagat
     7261 gagttgacag gtcgtagacg ttcaagtaag aaaaagaaga aaaaaacatc atttttacct
     7321 ttattttatt ttatcctttt tgctttatcg attttaattt ttgtgactta ttatgtttgg
     7381 aactatattc aaactcaacc agaggagcct tctctttcta attacagtgt ggttcaatca
     7441 acaagttcaa ctagctctgt tccccactcc tcaagtagta gttcttctag tatagaatca
     7501 gctataagtg tatcaggcga aggaaatcat gtagaaatcg cttataagac aagtaaggaa
     7561 acagttaaat tgcaattggc agtttcagat gttacaagtt gggtcagtgt ttcagaaagc
     7621 gaacttgagg gcggtgtaac cttatcgcca aagaagaaaa gtgcagaagc aacagttgca
     7681 actaaaagtc ctgtaacaat tacgttaggt gttgtaaaag gtgttgattt gacagtagat
     7741 aatcagactg ttgatttatc gaaattaaca gctcagactg gacaaatcac tgtaaccttt
     7801 actaaaaatt aaggaaaaac gaatgaaaaa agaacaaatt cccaatctct taacaatagg
     7861 tcgaattctc tttataccta tttttatctt tattttaacg ataggaaatt cgatagagag
     7921 tcatatagtt gcagctatta tctttgctgt tgccagtatt accgactatt tagatggata
     7981 tttagctcgt aaatggaatg tggtcagtaa ttttggtaaa tttgcagatc ctatggcgga
     8041 taagttacta gttatgtcgg cttttattat gttgattgag ttaggtatgg ctccggcttg
     8101 gattgttgca gtgattatct gtcgtgagtt agctgtgaca ggtttaaggc ttttattggt
     8161 tgaaactggt ggaacaattt tagcagcagc aatgcctgga aaaattaaaa cttttagtca
     8221 gatgtttgct attattttct tgctattaca ttggactttg cttggtcaag ttctacttta
     8281 tgtagcctta tttttcacta tctactctgg ctatgactat ttcaagggta gtgcctatgt
     8341 atttaaaggg acatttggtt cgaaatgaaa tcaataattg atgtaaaaaa tctttctttt
     8401 cgctataaag aaaatcagaa ctactacgat gtgaaggata ttacgtttca cgtgaaacgt
     8461 ggagaatggc tttcgattgt agggcataat ggtagtggta aatcaactac ggttcgatta
     8521 attgatggct tactggaagc agaatccgga gagattgtaa ttgatggcca acgtttgact
     8581 gaggaaaatg tttggaatat acgtcgtcaa atcggtatgg tttttcaaaa tccagacaat
     8641 caatttgttg gagcgactgt tgaagatgat gttgcttttg gtttggaaaa tcagggactt
     8701 tctcgtcaag aaatgaaaaa gagagtggaa gaagctctgg ctttagttgg catgttggac
     8761 tttaaaaaga gagagccagc gcgtctatca ggtggtcaaa agcaacgtgt ggccattgca
     8821 ggtgttgtag ccctaagacc agctatttta atcttagatg aagcaacgag tatgttggat
     8881 cctgaggggc gtagagaact tattgggaca gtaaaaggaa ttcgaaaaga ctatgatatg
     8941 acggtcattt ctattaccca tgatttggaa gaagtcgcca tgagtgatcg cgtattggtc
     9001 atgaaaaaag gggaaattga atcaactagt agtccaaggg agcttttctc tcgaaatgat
     9061 ttagatcaaa ttggattaga cgatcctttt gctaatcaat taaaaaaatc tttgagccag
     9121 aatggctatg atttacctga aaattatttg acagaaagtg agctagagga taagctatgg
     9181 gaattgctct agaaaatgtg aattttacat atcaagaagg tactccctta gcttcagcag
     9241 ctttgtcgga tgtttctttg acgattgaag atggctctta tacagcttta attgggcaca
     9301 caggtagtgg taaatcaact attttacaac tcttaaatgg tttattggtg ccaagtcaag
     9361 ggagtgtgag ggtttttgat accttaatca cctcgacttc taaaaataaa gatattcgtc
     9421 aaattagaaa acaggttggc ttggtatttc agtttgctga aaatcagatt tttgaagaaa
     9481 cggttttgaa ggacgttgct tttggaccgc aaaattttgg agtttctgaa gaagatgctg
     9541 tgaagactgc gcgtgagaaa ctggctctgg ttggaattga tgaatcactt tttgatcgta
     9601 gtccgtttga gctgtcaggg ggacaaatga gacgtgttgc cattgcaggc atacttgcca
     9661 tggagccagc tatattagtc ttagatgagc caacagctgg tctagatcct ctagggagaa
     9721 aagagttgat gaccctgttc aaaaaactcc accagtcagg gatgaccatc gtcttggtaa
     9781 cgcatttgat ggatgatgtt gctgaatatg caaatcaagt ctatgtaatg gaaaagggac
     9841 gtttagtaaa ggggggcaaa ccaagtgatg tctttcaaga cgttgttttt atggaagaag
     9901 ttcagttggg agtacctaaa attacggcct tttgtaaacg attggctgat agaggcgtgt
     9961 catttaaacg attaccgatt aagatagagg agttcaagga gtcgctaaat ggatagtatg
    10021 attttggggc gttatatccc aggggattcg attgttcacc gattggatcc acgtagcaaa
    10081 ttgcttgcta tgatgctact gattttgatt gtgttttggg ctaataaccc cttgacgaat
    10141 ctgattcttt ttatagcgac agggatattt attgccttgt caggagtatc tctttcattt
    10201 tttattcagg gcttgaagtc tatgtttttc ttgattgcct tcacaactat ttttcaacta
    10261 tttttcattt ctaatgggaa tgttttattt gagttttcgt ttgtgagaat cacggattat
    10321 gctttgcaac aagctgggat tattttttgt cgctttgtat tgattatttt cttttcaact
    10381 ttgttaacct taacaaccat gcctttaagt ttggcatcag ctgtcgaagc tttattagca
    10441 cctttaaagc gtgtgaaagt tccagttcat gaaattggat tgatgctgtc tatgagtttg
    10501 cgttttgtcc caaccttgat ggatgatacg actcggatta tgaatgcaca gaaagctcgt
    10561 ggagtggatt ttggagaagg aagcatcgtt caaaaagtaa aggcgatgat tcccattttg
    10621 attcctcttt ttgcgacaag tttaaaacgt gcagattcct tggctatcgc tatggaagcg
    10681 cgtggctatc agggtggaaa aggcagaagt caataccgac aattgaaatg gactctaaag
    10741 gatacgctga ccattcttgt tattctcgta cttggttgtt gtttattttt cttaaaatct
    10801 tagtagcttt caggaattga taaaaagtta ctgtaacagt ttttgatata aaggtaggga
    10861 tatgaaccgt tttaaaaaat caaaatatgt cattattgtt tttgtcactg ttctgcttgt
    10921 gtcagctctc ttagcgacga cttattcaag tacaattgtg acaaaattag gagatggaat
    10981 ctcattggtt gatagagttg tacaaaaacc ttttcagtgg tttgattctg tcaaatcaga
    11041 tttggctcat ttgacacgaa catataatga aaatgaaagt ttgaagaaac agctttacca
    11101 attagaagtt aaatcaaatg aggtggaaag tttaaagaca gaaaatgaac aactgcgcca
    11161 attgcttgat atgaagtcta aattgcaagc cacaaagact ttagcagcag atgttattat
    11221 gcgttctccg gtatcttgga agcaggagtt gaccttagat gcaggtagat caaaaggtgc
    11281 ttctgagaac atgttagcta ttgcaaatgg tggcttgatt gggagtgttt caaaagtaga
    11341 ggagaactct actatagtca accttctgac aaatacggaa aatgctgata agatttctgt
    11401 taaaattcaa catggctcta ctacaattta tggaattatt attggctatg acaaggaaaa
    11461 tgacgttctt aaaattagcc aattaaatag taatagcgat attagtgcgg gagataaggt
    11521 gactactggt ggattaggaa actttaacgt tgctgatatt cctgttggtg aagtggttgc
    11581 cacaacgcat agtacagact atttgacacg agaagtaact gttaaattga gtgcagatac
    11641 tcataatgta gatgtgatag aattagtggg gaattcataa tgagacagtt gaagcgagtt
    11701 ggagtatttt tattgcttcc tttctttgtt ctaattgacg cccatattag ccagcttctg
    11761 ggctcatttt tcccccatgt acatttggct agtcattttc tttttctatt tctcttattt
    11821 gagacgatag aagtatcaga gtatctctac ctagtctatt gttttgttat aggcttggtt
    11881 tatgatgttt actttttcca tctaataggg attacaactc tcttatttat cttattggga
    11941 gccttccttc ataaattgaa tagtgttatt ttgttgaatc gttggacaag aatgctagct
    12001 atgattgtgc tgacattcct gtttgaaatg ggtagttatc ttttggcttt tatggtaggg
    12061 ttgacagtag atagcatgtc gatttttata gtctatagct tggtaccgac gatgatttta
    12121 aattttttat ggattactgt ttttcaattt atttttgaaa aatattatct ataagaacga
    12181 catataaatg taacaaaggc gtaatattta ttaggccttt ttttggtata ctagtattgt
    12241 ctttaaaaga aggagtatct acgtaatatg aagaaaaaaa tcttagcgtc acttttatta
    12301 agtacagtaa tggtttctca agtagctgtt ttaacaactg cgcatgcaga aacgactgat
    12361 gacaaaattg ctgctcaaga taataaaatt agtaacttaa cagcacaaca acaagaagcc
    12421 caaaaacaag ttgaccaaat tcaggagcaa gtatcagcta ttcaagctga gcagtctaac
    12481 ttgcaagctg aaaatgatag attacaagca gaatctaaga aactcgaggg tgagattaca
    12541 gaactttcta aaaacattgt ttctcgtaac caatcgttgg aaaaacaagc tcgtagtgct
    12601 caaacaaatg gagccgtaac tagctatatc aataccattg taaactcaaa atcaattaca
    12661 gaagctattt cacgtgttgc tgcaatgagt gaaatcgtat ctgcaaacaa caaaatgtta
    12721 gaacaacaaa aggcagataa aaaagctatt tctgaaaaac aagtagcaaa taatgatgct
    12781 atcaatactg taattgctaa tcaacaaaaa ttggctgatg atgctcaagc attgactacg
    12841 aaacaggcag aactaaaagc tgctgaatta agtcttgctg ctgagaaagc gacagctgaa
    12901 ggggaaaaag caagtctatt agagcaaaaa gcagcagctg aggcagaggc tcgtgcagct
    12961 gcggtagcag aagcagctta taaagaaaaa cgagctagcc aacaacaatc agtacttgct
    13021 tcagcaaaca ctaacttaac agctcaagtg caagcagtat ctgaatctgc agcagcacct
    13081 gtccgtgcaa aagttcgtcc aacatacagt acaaacgctt caagttatcc aattggagaa
    13141 tgtacatggg gagtaaaaac attggcacct tgggctggag actactgggg taatggagca
    13201 cagtgggcta caagtgcagc agcagcaggt ttccgtacag gttcaacacc tcaagttgga
    13261 gcaattgcat gttggaatga tggtggatat ggtcacgtag cggttgttac agctgttgaa
    13321 tcaacaacac gtatccaagt atcagaatca aattatgcag gtaatcgtac aattggaaat
    13381 caccgtggat ggttcaatcc aacaacaact tctgaaggtt ttgttacata tatttatgca
    13441 gattaattta cagagggact cgaatagagc cctcttttca ggttttaccg tgacaatccc
    13501 tattaaaaat tatatcaaaa tagcttgaaa atattggaaa agtatggtag aatgaaaatt
    13561 gtcgtgtgaa cgataatact cattcttgat gaattgtgaa gcagttgccc ttgggtcgtt
    13621 ttgcgagttg aagtcaagaa gaggaaaaaa acaaaaagga gaaatactca tggcagtaat
    13681 ttcaatgaaa caacttcttg aggctggtgt acactttggt caccaaactc gtcgctggaa
    13741 tcctaagatg gctaagtaca tctttactga acgtaacgga atccacgtta tcgacttgca
    13801 acaaactgta aaatacgctg accaagcata cgacttcatg cgtgatgcag cagctaacga
    13861 tgcagttgta ttgttcgttg gtactaagaa acaagcagct gatgcagttg ctgaagaagc
    13921 agtacgttca ggtcaatact tcatcaacca ccgttggttg ggtggaactc ttacaaactg
    13981 gggaacaatc caaaaacgta tcgctcgttt gaaagaaatt aaacgtatgg aagaagatgg
    14041 aactttcgaa gttcttccta agaaagaagt tgcacttctt aacaaacaac gtgcgcgtct
    14101 tgaaaaattc ttgggtggta tcgaagatat gcctcgtatc ccagatgtga tgtacgtagt
    14161 tgacccacat aaagagcaaa tcgctgttaa agaagctaaa aaattgggaa tcccagttgt
    14221 agcgatggtt gacaccaata ctgatccaga tgatatcgat gtaatcatcc cagctaacga
    14281 tgacgctatc cgtgctgtta aattgatcac agctaaattg gctgacgcta ttatcgaagg
    14341 acatcaaggt gaggatgcag tagcagttga agcagaattt gcagcttcag aaactcaagc
    14401 agattcaatt gaagaaatcg ttgaagttgt agaaggtgac aacgcttaat ttatacaaat
    14461 agtaattacc taggagggcg gggcttagcc cggctctcct attttcaaaa aatataggag
    14521 aattaaaatg gcagaaatta cagctaaact tgtaaaagag ttgcgtgaaa aatctggtgc
    14581 cggtgttatg gacgctaaaa aagcgcttgt agaaacagac ggtgacatcg aaaaagcgat
    14641 tgaattgctt cgtgaaaaag gtatggctaa ggcagctaag aaagctgacc gtgttgctgc
    14701 agaaggtttg actggtgttt atgttaacgg taatgttgca gcagttattg aagtaaacgc
    14761 tgaaactgac ttcgttgcaa aaaacgctca attcgttgaa ttggtaaata ctacagctaa
    14821 agtcattgct gaaggaaaac ctgctaacaa tgaagaagct cttgctttga taatgccttc
    14881 aggtgaaact cttgaagctg catacgtatc tgcaacagca actatcggag agaaaatctc
    14941 attccgtcgc tttgcattga ttgaaaaaac agacgcacaa cactttggag cataccaaca
    15001 taacggtgga cgtatcggtg ttatttcagt tgttgaagat ggagacgaag cacttgctaa
    15061 acaattgtca atgcacatcg cagcgatgaa accaacagtt ctttcttaca aagaattgga
    15121 tgagcaattc gttaaagatg agttggcaca attgaatcac gttatcgacc aagacaacga
    15181 aagccgtgca atggttaata aaccagctct tccacacttg aagtatggat caaaagctca
    15241 attaactgat gatgttattg ctcaagctga agctgacatc aaagctgaat tggctgcaga
    15301 aggcaaacca gaaaaaatct gggacaaaat tattccaggt aaaatggatc gcttcatgct
    15361 tgataacact aaagttgacc aagcttacac acttcttgca caagtttaca tcatggatga
    15421 cagcaagaca gttgaagcat accttgaatc agttaacgct tcggtagttg agtttgctcg
    15481 ctttgaagtt ggtgaaggta tcgagaaagc tgcaaacgac ttcgaagctg aagttgcagc
    15541 tacaatggca gcagccttga ataactaata atagaaagga agcaaattgc ttcctttttt
    15601 gtatagggct tagatttttc ctgaaattgt tttttcccca gtctcactcg attagaataa
    15661 ttaaaacaaa aagccctata ataagggcta agtgcattta ggagactata cttcaaattc
    15721 atagtaagcg cgtcataaca aggtatctat cctaattccc caacttccag tatactagat
    15781 aaaaaataat tatcggagga aaggtattgt actatgataa ttcaactaag tgatttaggt
    15841 caagttcacc ttgtttgtgg caagacagat atgaggtagg gaatagactc attagcctat
    15901 gtagttaaaa cccactttga attggattct ttctccggtc aagtctttct cttttgtggt
    15961 ggacgtaaag accgctttaa agccctttac tgggatggtc aaggattttg gctactatat
    16021 aaacgctttg agaacggaaa actgacttgg ctaagtacag aaaaggatgt caaagctctc
    16081 gcacctgaac aagtagattg gcttatgaag ggcttttcta tcactccaaa aatatagtag
    16141 attgaaacta gaatagtaca cctctgcttc taaaacattg ttagaaatcg atttgattgt
    16201 cctgttctta tttcatttta ctataaatcc atcagaaagt tgtgatttct attgaaatga
    16261 ggactttctt tttacactca tctgctttca aaaagcattc tagtccatct ccgattaacg
    16321 atggacttta tcacctcctt ctccagtcct tgtatgacat cttccaaagt tcgaaaggct
    16381 ttattcttaa atccacgttt acgaatctct ttccacactt gttcaatggg gttcatctct
    16441 ggtgtgtatg gaggaataaa tgcaaagcca atattagtcg gaatctttaa ggtacttgat
    16501 ttatgccata tagcattgtc cataacgagt aaaagataat catctggata agcttgtgaa
    16561 agctcctatt cctaaagccc ctttagcgca tagctttggc tcagcttcta ttatcgctca
    16621 caccatccat cagaagttta atctgaaggt acccaattat cgccaagaag aagattgggc
    16681 taagatgggt ttaccaatca cacgcaagga aatctctaat tggcatatca agacgagtca
    16741 atactatttg gagcccctct ataacctctt gcgagagaga ctattgactc agcccttact
    16801 tcatgcggat gaaacttctt atagggtgct agagagtgat agtcagctga cttactattg
    16861 gacttttttg tctgggaaag ctgagaaaca agggattacg ctttaccatc atgtcctaat
    16921 cgatttgttc atatcttatt tcaatccatt ataaatagtg agaaatatct atcctatctt
    16981 ctagaatgtc ttccaaacgt ggaaactctc gtaaacaaag a
//
LOCUS       DAVLXF010000036        16735 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLXF010000036 DAVLXF010000000
VERSION     DAVLXF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.036"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(108..1742)
                     /locus_tag="VYO12_001152"
     CDS             complement(108..1742)
                     /locus_tag="VYO12_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587774.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLPILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1723..2472)
                     /locus_tag="VYO12_001153"
     CDS             complement(1723..2472)
                     /locus_tag="VYO12_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587775.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            2651..4105
                     /locus_tag="VYO12_001154"
     CDS             2651..4105
                     /locus_tag="VYO12_001154"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1587776.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANKELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4195..6813)
                     /gene="alaS"
                     /locus_tag="VYO12_001155"
     CDS             complement(4195..6813)
                     /gene="alaS"
                     /locus_tag="VYO12_001155"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1587777.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6835..7320)
                     /locus_tag="VYO12_001156"
     CDS             complement(6835..7320)
                     /locus_tag="VYO12_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1587778.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7595..7834)
                     /locus_tag="VYO12_001157"
     CDS             complement(7595..7834)
                     /locus_tag="VYO12_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587779.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLNDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8095..9165)
                     /locus_tag="VYO12_001158"
     CDS             complement(8095..9165)
                     /locus_tag="VYO12_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1587780.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYEAFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9162..9935)
                     /locus_tag="VYO12_001159"
     CDS             complement(9162..9935)
                     /locus_tag="VYO12_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587781.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9932..10738)
                     /locus_tag="VYO12_001160"
     CDS             complement(9932..10738)
                     /locus_tag="VYO12_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587782.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10719..11876)
                     /locus_tag="VYO12_001161"
     CDS             complement(10719..11876)
                     /locus_tag="VYO12_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587783.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(11935..12885)
                     /gene="murB"
                     /locus_tag="VYO12_001162"
     CDS             complement(11935..12885)
                     /gene="murB"
                     /locus_tag="VYO12_001162"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1587784.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(12997..13749)
                     /locus_tag="VYO12_001163"
     CDS             complement(12997..13749)
                     /locus_tag="VYO12_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1587785.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13759..14490)
                     /gene="budA"
                     /locus_tag="VYO12_001164"
     CDS             complement(13759..14490)
                     /gene="budA"
                     /locus_tag="VYO12_001164"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1587786.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14632..15903
                     /locus_tag="VYO12_001165"
     CDS             14632..15903
                     /locus_tag="VYO12_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1587787.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15923..>16735)
                     /locus_tag="VYO12_001166"
     CDS             complement(15923..>16735)
                     /locus_tag="VYO12_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1587788.1"
                     /translation="KSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVVA
                     TSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGKA
                     DLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGTV
                     PESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATVS
                     LKMKDGDANAVALRKNSDDLKEVVDKVIQKLKNEGTYQSYLEKAASLTEVEE"
BASE COUNT         5030 a         3780 c         3315 g         4610 t
ORIGIN      
        1 ttcctcccta tatttttgat taccaatctt aatcatttac aactacattc taacaaacta
       61 taaaagcgtt tgtcgaattg aatttatcaa gcaagcaacc aactatctca tcttttttct
      121 atttctgcca atatgcgtga caggtaataa tgatagccaa aaatagcaag agcaagcaag
      181 acgataagag ctcctactcc caagttgatg gcaaggatag gggagagaga ctgaaccaag
      241 aatatgctcc caattacaag ggccatcagg attgcactat aaataaacaa taaaactatg
      301 gcgactatgc catttgaacg attcaccagg tccgtaatgc tactccaatt ggttgacaga
      361 tttttaacgt ccttaaagta atggtggcaa gaaaggatga cactggcaat gatccagact
      421 acaagaaggt aaatcatcga aaggatgggc aagcctagat atagagaaag accgagcaaa
      481 gtcagaactg gtaaaaagga ctgaacagca aagataatcc aaaatttcac tttcacataa
      541 cgagcaaagt caaagggtaa actcttcaga aaatcaacat tttccctctc caaggacaag
      601 gcaattgaat gcaggctggt gatattgtta ttgacaactg ctataaacac tcctacaaaa
      661 aacaagggta accagtatgg aggatgaatg tctggaacta tctgagaatc tcggattttg
      721 gaaatcagac tgatcatcat gagataagga aggaaagcac ttgtaaaaag cactgtaatc
      781 acaccagtcc cctgtcccaa gagggtgagg tggtagcgta aaaccatacg gaaaaagccc
      841 tttttagtag ttgaaatcct ctccttgctg cgacgttctt ttttgacctt ctcctcactg
      901 ttaagcagga tcacgtcata aaaacgagga aggaccttct ttttggtcag ataaagcagg
      961 aagagagtta gtcctatcca agcgagcaga cccactaagg cttctgtcga aaaaggctcc
     1021 actgctattt tgtaaaagat atgaagagga taaaggagaa atggaatgtc tctaactttg
     1081 tcaacaatac ttccaaaagt cgactgaaga aagaagataa atattaaagg tatgagaact
     1141 cctatcccaa tcatcacatt cgaaaaaata gactgatact ttctgaagac cctagtctga
     1201 gccaagaaat gtactgccac taccgtcact aaagtaacag agacaaataa taaggtcaag
     1261 gacagtagca tcaaaggcaa acccagccaa agagaaggag ctagactaat atagagggct
     1321 agaaaataag ctaggattgg tacaattcca gttagagctg gcaagaggac agacagacct
     1381 ttggccatta taatctctga ttctttaaag gcatagggcc tataggaggc caggtcctta
     1441 ctctcataaa agacattgta aaaggcagtg aaagaagttg aaaaggccag caccagtaaa
     1501 atagcaacca tcgagctaaa ataaataggc atttcctcaa aaggaaaatg aatggctata
     1561 ttactaaaac agatgagcat caagagactg gaaaaaatgt aagaacttag gactctagca
     1621 gaaacattta cttttttccc aggattctta gcctgcttct ttcgtagatt agccagatta
     1681 gcttcttgag atgaatagag gatattgata tcaactaatt ttttaatgac cttgagacgc
     1741 atccgcaacc tcctcttttc taccagcaag actaaggtag atactttcca aaaactggtc
     1801 tgggtggtct ttcctcaagt tctctacact accacaatag atcaaatgcc cctttttcaa
     1861 aatagcaatc cgatcacaga cttgctcggc tacctcaagg acatgagttg aaaacaagac
     1921 cgtcttgcct ttttgtgcat gttccttcat catttgcttc aaatcaaagg cggcctgggg
     1981 atccaaacca gtcaagggtt cgtccaaaac ccaaatatca ggagcagaca agagtgcccc
     2041 aatgacaaag actttctgac gcattccgtg agaaagagtt tcaataacct gatagcggtt
     2101 ttcagcaaaa tcaaaaacgt tcaatagcct agctagacta gtctccaagt cagagctagt
     2161 cagatcatag gatgaagcga tcaattccca aaattcattg gccgttaagc gtaaaaataa
     2221 gtcaggcgag tctgctacgt agccaatctt tcgtttgatc gccaagcgat tttccgataa
     2281 ctcctgaccg tctaccaaaa tacgaccact gctgggtgaa atgatactga ctagggattt
     2341 tatggtggtc gattttccag ccccattatg accaatcaag cccataatct ctccattttc
     2401 aatctgcaaa ttgagattac tcaaggcctc tttatcacca tacaacttac taacattttg
     2461 aaattcaatc atgggacaac tcctatcttt atctgtatta tacatcgcta tactagcact
     2521 ttgatacaaa aaaatcaata ctttatttta agtagttaaa atagtttcct tctatcttac
     2581 gcaaacgttt gcgccgagag atactttatg atagaataaa gaacaagatt gacaagtaag
     2641 aggaaacatc atgcaaaatc aaacactcat gcaatacttt gaatggtatc tgccccacga
     2701 cggtcaacac tggacgcgtc tggctgaaaa tgctccacac ctagctcatc tggggatcag
     2761 tcacgtctgg atgccaccag ccttcaaggc aaccaacgaa aaagatgtcg gctatggggt
     2821 ctatgactta tttgacttag gagagttcaa ccaaaaaggg actgtccgca ccaagtatgg
     2881 tttcaaagaa gactatcttc aagccattca agcccttaaa gaacagggaa ttcaacctat
     2941 ggccgatgta gttctcaacc acaaggctgc tgccgataac agggaagcct ttcaggttat
     3001 cgaagttgat cctgtagacc gtacagttga acttggagaa cccttcacca tcaatggctg
     3061 gactagtttt accttcgatg gtcgccaaga tacctataat ggcttccact ggcattggta
     3121 ccacttcacc ggtacagact acgatgccaa acgcagtaaa tctgggattt atctgatcca
     3181 aggggacaac aagggctggg ctaacaagga attggtcgat aacgaaaacg gaaactacga
     3241 ctacctcatg tatgccgacc tagactttaa acatcctgaa gtcatccaaa acatctatga
     3301 ctgggctgat tggttcatgg aaacgactgg tgtagctggt ttccgtttgg atgccgttaa
     3361 gcatattgac tctttcttta tgcgcaactt catccgcgat atgaaggaaa aatacggtga
     3421 cgatttctat gtttttggtg aattttggaa cccagacaag gaagccaatc tggactatct
     3481 cgaaaaaacg gaagaacact ttgaccttgt cgatgttcgt ctccaccaga atctctttga
     3541 agccagtcaa gctggcgcaa actatgacct tcgtggcatt ttcacagata gcctggttga
     3601 actcaagcct gacaaggctg tgacttttgt cgacaaccac gatacccaac gaggacaagc
     3661 ccttgagtct accgttgaag aatggttcaa gccagcagcc tatgccctca ttttgttacg
     3721 ccaagacggc cttccatgtg tcttttacgg agactactat gggatttcag gccagtatgc
     3781 tcaagaagat ttcaaagaaa tccttgaccg cctcctagcc atccgaaaag acttggccta
     3841 tggagaacaa aatgactact ttgaccatgc taactgtatc ggttgggtac gttcaggtgc
     3901 tgaaaatcaa tccccaatcg cagtccttat ctcaaatgac caagaaaaca gcaagtcaat
     3961 gtttgtcggt caagaatgga ctaatcaaac ctttgtagat ttacttggta accaccaagg
     4021 tcaagttaca attgatgagg aaggttatgg acaattccct gtctcagcta gatccgtaag
     4081 tgtctgggca gtcaatacca tctaatagct cataataacc aagctaggtc caagcggatt
     4141 tggctttttt gtattcacaa aaagacctac ccaaatggat agatctttac ttgattacaa
     4201 tttacctgct actgcatcca acaattcttg gatcttagct tggttgcttc ctcctgccat
     4261 ggccatgtct ggtttaccac caccacgtcc atcgatgatt ggtgctaatt ctttgacaag
     4321 gtttcctgca tgaaggtctt ttgtcttgct tgctacaagg acattgactt tgtcaccgat
     4381 agcggcaact aggacaagaa gatcagagta gtctttttgt ttccagttat ctgcaaaagt
     4441 acgaagggca ccggcatcgg atacagacac ttgactagca atgtaacgat gaccgttgac
     4501 ttccttaaca tctttgaaga tatcgcctgc ggctgcagct gcggcttttt ctttcaactc
     4561 agcattttct ttttgaagtt gacgaagttg ttcttgaagt ccttctacct tgtgaggtac
     4621 ttccttgact tgaggtgctt tcaaggttgc tgcgatagct ttaagagcat cctcttgttc
     4681 acgataggct tcaaaggctt ccttaccagt cactgccaag atacggcgag ttcctgaacc
     4741 gattccttct tctttgacaa ttttgaagag accaatctca gaagtgttgt caacatgagt
     4801 accaccacaa agttcaatag agtagtcacc gatagtcacg acacgaactt ccttgccgta
     4861 tttctcacca aagagggcca tagctcccat ttctttagca gtgtcaatat ccgtttcaac
     4921 tgtcttcact tcaagtgctt cccaaatttt ctcgttaact tgctgttcaa tcgcacgaag
     4981 ttcctcagca gttactgctt ggaagtgggt aaagtcaaag cgaaggaatt caacttcgtt
     5041 aagagatcct gcctgtgttg cgtggtttcc aaggatattg tgaagggcag cgtgaagcaa
     5101 atgagtcgca gtgtggtttt tcatgacacg gtgacggcga ttgctatcaa ttgccaaggt
     5161 atattcttgg ttcaaggcaa gcggtgcaag gacttcaact gtatgaaggg cttgaccatt
     5221 tggggctttc tgaacattgg tcacagtagc cacaatctta cctgactcat ccaagatttg
     5281 tccgtagtca gctacctgtc cacccatttc agcataaaat gacgtttccg caaagataag
     5341 agaggcagtt ccttctgaaa cagctcctac ttctgcattg tcagcaacga tagctaccaa
     5401 tttagaagac aattggctag tattgtagtt gaagacactt tctacagtga tgttttgaag
     5461 agtttcattt tgcataccca ttgagccacc cttgacagct gacgcacgcg cgcgttcttg
     5521 ctgttctttc atggctgctt caaaaccttc acggtctaca gtcataccag cttcttcagc
     5581 gatttcttca gtcaattcaa ctgggaaccc ataagtatca tagagtttaa agacatcttg
     5641 accagcaatg acagattgac ctttttcttt caagtctgcc acaatgcctt gggcaaagtg
     5701 ttgacctgag tgaagggtac gggcaaatga ttcttcttcg ctcttaacga ttttctcaat
     5761 aaagtcacgt ttctcaagca cttctgggta gtagctttcc atgatttttc caacagttgg
     5821 aaccaatttg taaaggaaag gctcgttgat acccaatttt tgaccatgca tagaagcacg
     5881 acggagaaga cgacgaagaa cataaccacg accttcattt cctggaaggg caccatcacc
     5941 gatagcaaat gaaagagaac gaatgtggtc tgcgataacc ttgaagctca tgttgtcgcc
     6001 atcttggtca taaaccttac cagacaattt ctcgacttca cggataatcg gcatgaagag
     6061 gtccgtttca aagttggtct tagccccttg gataacggcc accaaacgct ccaaaccagc
     6121 gcccgtatca atgttcttat gtggcaattc cttgtattcg ctacgaggaa cagcagggtc
     6181 tgcgttaaat tgtgacaaaa cgatgttcca gatttcaata taacggtcgt tttcaatatc
     6241 ttctgcaagc aggcgaagac cgatattttc tgggtcaaag gcttccccac ggtcaaagaa
     6301 gatttctgta tctggtccag aaggtcccgc accgatttcc cagaagttgt cctcaattgg
     6361 aatcaagtga cttggatcca ctcccacttc aatccagcgg ttgtaagaat ctttatcgtc
     6421 tggatagtag gtcatgtaaa gtttttcagc agggaaatca aaccattcag ggcttgtcaa
     6481 aagctcataa gcccaagtga tagcttcgtc acggaagtaa tccccgatag agaagttccc
     6541 cagcatttca aacatggtat ggtgacgcgc agtcttccct acgttttcga tgtcgttggt
     6601 acggatagcc ttttgggcat tggtaatacg tggattttca gggataatgg tcccgtcaaa
     6661 gtatttctta agggttgcta ccccagagtt gatccacaaa agagttgggt catttacagg
     6721 aaccaaactt actgatggtt ctactgagtg acctttggtc gcccagaaat caagccacat
     6781 ttggcgtact tgtgcactag atagttgttt catattgtct ccttattcac ttgtttaatg
     6841 tgattggctt tccagcattt ccacatagtc aatcgcgaca cagagggaaa tgactaggtc
     6901 tgcataagcg tcttcaagaa ccgttacggt ataggtagag gtcagatgga agagttcctt
     6961 cttaatttcc gcaatcaact gatcgcgatc atccagcaat ttgaaattca aatcccagat
     7021 attgccctcg atacgaagac ctagattatc aaactcatac ttatctcgcc agaaggtcaa
     7081 cttcttacga atgacaaaac tcgagccatc ccgaagctga atctcaaaac gaggaagcaa
     7141 ggtcaagatt tctttactga tctgactgac ttgttcacca gccgcatcat agatggtaaa
     7201 agttttggga atcttaaaaa atgatccctc cacctgatag gcaatttctc ccctgtcatc
     7261 cttgatagcg aagcgttcgc ctccaagacg aaacttttgt ttgacaagaa atgttttcat
     7321 caacacctcc aaaaatcaaa agacaagctc atatcacgaa gggcgaaaaa ccgcggtacc
     7381 accttcattc aatgaacttg tcattctctt gttcttatgc aattgtatga ttgagtagca
     7441 tgacttccta gcttagatgg ctcgcagcac cgccatttct ctggactaag acaagtgaaa
     7501 atcaattctc aactttctta ttataacgtt tttttaagct tgcgtcaact ggaaatgatc
     7561 tccgttgaat tagaccaatt ccctacatct ctgattactt tttcaggata tattttttct
     7621 tactgccatt tttcttttta tcccaaattt tcatattact aaacacagct actagaatat
     7681 ttccaaatat aaaggtgcct atcacccaat atatggactc agttgttagg tattgtcgat
     7741 ccaagccatc atttaaatgg aatagtatag cagtttggtt aacaatcata aaggttggcc
     7801 agaaactttt tttgaaaaaa gtagacattt tcattatttg ttgccgcttt ctgtaaggtt
     7861 aatactcaat aaaaatcaaa aagcaaacta ggaagctagc ttcaagctgt acttgagtac
     7921 ggcaaggcaa cgctgacgtg gtttgaagag tataggctta gtatactact aggcaagcaa
     7981 ataagcaaat aaacaaataa acaactagaa tagaaaaaga tagggctcta aaaactgact
     8041 tctattcctt aaaaacgaac cagcttgact gattcgtctt cttacgttta tctcctactt
     8101 ccgatacatt ttaaactgta ggaagaggtc gctatatttc cctgtccatt tatggtcaaa
     8161 tttctcataa acttctaggt gtttcatggt ttcaacatcg ggatagaagg ccttatcttc
     8221 ctttgtttcc tctgggagca attccttcgc tggtaggttt ggtgttgaat agccgacata
     8281 ctccgcattt tggagagcat tttcaggttt caacataaag ttgataaagg cataggctga
     8341 gtcttgattt ttaactgttt tgggaatgac catattgtca aaccaaagat tgctggcctc
     8401 tgtcggtacc acataacgta gattttcatt tttttctaac atttggctgg cttcaccaga
     8461 gaaggtcacg ccgattgcaa cattattctg aatcatgtag cccttcatct cgtccgcaac
     8521 gatagccttg atatttggag tcagtttgta gagcttatcc actgtctctt ccaactgctg
     8581 cggatccttg gagttgaggc tgtagccgag ggaattgagt cctagtccca gcacctcacg
     8641 cgccccatca aaaagcatga tagaattctt atactccagc ttccaaaggt catcccaatg
     8701 ctcaggcgct tcatctacca tggtttcgtt gtagacaatt cctaaggttc cccagaagta
     8761 agggatggag aatttattac ctgggtcaaa ggactggttg agaaactctg gtccgatatt
     8821 ttcgattcct tcaatttttg aataatcaag cggaaccaag aggtcttcgt ccttcatctt
     8881 gttaatcatg tattcacttg gaatggcaat atcgtaggtc gttccaccct gctttatctt
     8941 agtgtacatg gcttcgttgg agtcaaaagc ctcgtactga acttgaattc ctgtttcttc
     9001 tgtaaactga gtcaagagtt caggatcgat atagtctccc cagttataga taaccaattt
     9061 ttgactatct cgactattga ttttactatc taaatgagtc gcaattcccc acaagacaag
     9121 gataatcgct gcaattcctg ctaaaaatga atagattttt ttcatgcttg ctcctccttc
     9181 tcacgagaga taaagtaata acctacaact aggataatac taaagagaaa gactagagca
     9241 gaaagggcat tgatttctag cgaaatcccc ttgcgagcac gagagtaaat ctcgactgat
     9301 agggttgaaa agccatttcc tgttacaaag aaggtcacgg caaagtcatc taacgaatag
     9361 gtgaaggcca tgaaataacc agcaatgata gacggagtca ggtaaggaag catgatttcc
     9421 ttaaacatct gaaattgact agctcccaag tcataggccg catgaatcat gtcgccattc
     9481 atttccttga gtcgaggcaa gaccatcaag accacgatag gaatggagaa ggccacatga
     9541 ctagatagaa cggtcaaaaa gccaagtgaa aacttgagtt gggtaaagag aatcaagaag
     9601 ctagcaccaa tcataacgtc aggcgcaacc atgaggatat tattgagtga tagaaaggct
     9661 tcttggtatt tcttacgaga ctggtagatg taaatggcac caaaagtccc gataatggtc
     9721 gctatcaagg ctgataggaa ggccaagaaa aatgtctgag ccaaaatcag catgagtctc
     9781 ccatctccaa acatggtttc aaagtgagtc cagctaaaac ctgtaaagct attcatatca
     9841 tcaccagcat taaaggcata gccaatcaag taaaagatag gcaggtagag gaccagaaag
     9901 accagtccca gataaaggtt ggcaaatttt ttcatcgttc tctcctttcc ttagtcaccc
     9961 acatggtgat gaacatggtc aggatgagaa tcacaccgat agttgaaccc ataccatagt
    10021 tgtcattggt tagaaaattc tgctcaatag ccgtccccaa ggtgataacg cggttcccac
    10081 caatcaaacg ggtcagcatg aagagactca aacttgggat aaagaccgac tgaaccccac
    10141 ttcgcacacc gttcatagat agagggaaga tgacatgacg gaaggtctcc cacttagttg
    10201 caccaaggtc gtaactagca ttgatgagat tattatccat atcgtctaag acattgaaaa
    10261 tcggcaaaat cataaagggg agctcgatgt agcttgcgac aaagataaag gaaaaatcgg
    10321 taaaaagcaa ctgttgtgaa ccaattccga taaattccaa gaattggtta atagagccat
    10381 tttgaccaaa aatcccgata aaagcatagg ctttaaggag caaattgatc caggtaggaa
    10441 ggataatcag catgagccag agttgacggt gcttgagacg ggtcaaaaag agggctgttg
    10501 gatagctgat aagcaaggtt acaaaggtca caatccctgc ataaagcact gagttgaaac
    10561 tcattttgag gtaggtcaag ttttgtgacg caaagtaaga tttataattt tctaaactaa
    10621 actggccttc aatgttgaaa aaggattgtc cgaaaatcaa gaccaagggt gccaatacaa
    10681 agagggcaat ccaaagcatg tagggcacta caaagagttt agagcttgtt ttcttcatct
    10741 ctttcctcct cgatcgcatt gatcagacct gcttcttgct cttcgatttc cacgtattct
    10801 tcgatacgag catcgaactc ttcttcggtt tcattgagac gcatgatgtg gatgtcttct
    10861 ggttcaaagt ccagaccgat ttcctcaccc acgatggcct tacgggttga gtggatcatc
    10921 cattcatttc caagttcgtc ataggcgata atttcataat gcaccccacg gaagagctgg
    10981 gtatcgacct taacttggag cttgccttct tcaggaaggg taatgcgcaa gtcctctgga
    11041 cgaataacga cctcaacagg ttcatttggc ttcatcccac catcaaccgc ttcaaagcgt
    11101 ttgccgttaa attcgaccaa gtagtcctca atcatggtac ctggcaagat gtttgactcc
    11161 ccgataaagg tggcaacaaa gtggttgatt ggctcatcgt agatgtccac aggggttcca
    11221 gactggacaa tctcgccatc attcataacg aaaatccagt cactcatggc aagagcttct
    11281 tcctgatcgt gagtgacaaa gacaaaggta atgcccaatc gttgttgtaa ttcacgcaat
    11341 tcgtactgca tgtctgttct caatttcaag tccagcgctg ataaaggctc gtccaacaag
    11401 accacacggg gttggttgat gatagcacgg gcgatggcca cacgctgacg ttgtcctcca
    11461 gaaagtttgc ggatggaacg tttttcataa ccttccaact gaaccatctt gagaacttcc
    11521 gctacacgct gctcaatttc tttcttatca attttacgca agcgaagtgg aaaggcaaca
    11581 ttttcaaaca cattcatatg tgggaacaag gcataggatt ggaagacggt atgtacgtcg
    11641 cgcttgttgg ttggaatatc attgatacga acaccgtcta gcatgatatc tcctgtcgtc
    11701 gcatccagta aacctgcaat aatgtttagg atagttgatt tccccgaacc agatgcacct
    11761 agaagggtgt agaatttccc ttcttccaac tcaaagttga tgtctttgag aaccttggtg
    11821 ttgctgtctt caaaaacttt agagacgttt ttgaattcga taattggttt tttcaattgg
    11881 cataaattcc ttctttttca tagattaacc gatcggggct ctgtcaggtc cccactacct
    11941 cttgcaggga gtaaaaccac ctgcatacat cttcgctacc gataggcttt cacccaagat
    12001 ccggacttct ctttcaagcg taatacctga gtgttccttg actttttcga taaccgattg
    12061 gatcaagtcc tcgtagtctt tggccgttcc atctgcgaca ttgatcataa atcctgcatg
    12121 cttttctgac acttctacgc caccgatacg atagcctttc aagccagctt ctgaaattaa
    12181 ctgacctgca aaatgcccga ctggacgctt aaagaccgag ccacaagatg ggtattccaa
    12241 aggttgcttg agttcacgta ggtgcgtcaa gcggtccatt tcctgcttga taacctgatg
    12301 ggttcctgga gctagggcaa atttaactga caagacaact gcaccagact cctgaatagc
    12361 tgaatggcgg taaccaaaag ccaagtcttt agcagacagg gtttcgattt ctccatcctt
    12421 ggtcaagacc ttacaagact gcaagatgtg agcaatctcg ccaccatagg cacccgcatt
    12481 cataaagaca gcaccgccaa cgcttcctgg aataccacaa gcaaactcaa agccagttaa
    12541 actatgacgg agggcaatgc gagttgtttc aatcaagtta gccccagctt ctgcttcaat
    12601 ggtatagcca tcaacagaaa cgttattgag cttgtcacac aagatgacaa atccacgaat
    12661 cccaccatca cgaacgatga tattgcttgc attgccaaga accatccaag ggatattttc
    12721 ttggttggca aatttcacaa cgcgagccaa ctcaaaacga tttcgtggaa agaccaaata
    12781 atcagcctct ccacctactt ttgtataact atagctatgc aagggttcct taaaacggat
    12841 atcaattcct tctaagattt caagcatttt ttctcttaca gacatgtcac tcttcctttt
    12901 acaaaattca ttccattata ccatttttag agacatttga cgaccataaa aataccttgt
    12961 ttggattttg cataagaaaa agaggttccc ccctttttat gattttttac aaaagatttc
    13021 cttggttcca taggcgacca gaacgagctc cagtgctaga atcacttcaa ccaagactgg
    13081 atttgtcaac cagcctactt ggaaaagaga tggtgccaga tcaaagaagg catgcaagcc
    13141 ataggctgct aggagataaa tccatttctt ctggcgaaca gcttggtaaa cccaaactgt
    13201 caaaagtaat tggaaaccaa gcgccaagat tcgctcaaaa ccaagcaaat aaatctgcca
    13261 gaccgaaagt gactgaatgg tttttaacat attttcagac agtaattgca taacctgtgg
    13321 attctgagtt tgaactgccg aaagaacgat gtaaagattg agtaaactaa taaggcctag
    13381 aaaaatcaac tccaagccac catgccctag gccataggcc aaggcatctg ccttttccaa
    13441 acttctcttt ttctccaacc atttgaagaa aacaagacga gccgtttcct caaaaaatgc
    13501 tgccatggct aggccataga taatatagat aagcggatga tcttgcaaga gggcaatact
    13561 accgtctttt tgaggatgca aaatcaagat atgcaccagt ttttccaaaa tctgcgaaga
    13621 gacaaagaaa gcgactgcac ccaagcccaa gacagctaga ttaatctggt atttcttttt
    13681 ggcaaaccaa atgcttccta tcagaaaagc tagcaacagc aacatggtaa taatgatatg
    13741 aatggtcatt ttcttctcct attctgcctt ttcaatatct tttttcatct cgtcaacatt
    13801 gaacttagca aacaagtatt gacggtcttg gactgggaaa cgttggtcca actggtcaac
    13861 tgctcccacc tcgataatgc cttccttgat gacaaaatcc atgacatgtc caccgaaggt
    13921 caaatcatct gatatgaagt gcagatggta gcctgccaca ctgaccccat ggaaaatctc
    13981 aggcgtccag aaaccaacaa tagtccccgc aacattgtca cgactatatt ccggttgatg
    14041 ggttgcgaca tcagcaaact tggtatcggg tgttgactta ggaatcatac gcacatgcat
    14101 atgtgaaaat tccccccgaa tcttgataga gcggaaaaga ttttccccat cataataaga
    14161 ctcaattcgt tctttcaatt ccttgtctgt catctcaaag cgctggcgga aaatgacctc
    14221 tgcctgatgc ggtaccactg cagcgtaagg aataagggca tctggtgaca cttccacaat
    14281 ttctggctgg tctcctgacc ctttggcctg ataagccttg ccatccaaga caatcaattc
    14341 cccatcaatg gaatccaagg ttcccaaacc aaggtcacca tgttctagca attctcctac
    14401 tgtcatagta ccaccataaa ggccagccat caaggctcca agagtattgt attgaaataa
    14461 tttcactggt tcctgcacgt tcttatccat tcactttctt gtctgttatt ctataaatct
    14521 tactcctaag tataccacat ttgcccctag atgtgaacga gagaaacgct ctagacattg
    14581 ccaagaagga aaaaaaaggg tacaatgtaa caaaatcaag ggaggtctgg aatgaagaaa
    14641 caaagcaagt acaaagaggt cgtttcctat ctgaaaaatg gtatcgagtc tgaacgattt
    14701 ccgacgggta gtcgcctgcc ttctatccgt caactgagcc ttgactttca ctgcagcaag
    14761 gacaccattc aacgagccct gctggaatta cggcacgaac aatacctcta tgccaagcct
    14821 cagagtggct actatgtatt agaacaaggg caacatcaag acctagaaat cgaggttacc
    14881 gacgaacatg ccagtgccta tgacgatttc cgactctgtg tcaatgaaac cttgattgga
    14941 cgagaaaact acctcttcaa ctactatgac aatcaagaag gattagaaga cctaagacag
    15001 tccattcaca aactcctatt tgagcaagct ctctactgca aggctaacca actagtactg
    15061 acttctggaa cccaacaagc cttgtttatc ctctctcaaa tatcctttcc tagacaagcc
    15121 aaggaaatct tggtggaaca gccaacctac catcggatga atcgcctctt gattgcacag
    15181 gggctggact atcaaacgat tgaacgaggc attgatggga ttgacttgga ggagctggaa
    15241 ggccacttca aaacaggaaa aattaaattt ttctacacca ttcctagatt tcactatcct
    15301 ctgggacatt cctattctga gcaagacaaa cgatctattc ttaacttagc tgccaagtat
    15361 gatgtctata tcgtagagga cgattatctg ggtgatttgg actccaagaa gggccaaacc
    15421 ttccactatc ttgatacaga ggagcatgtc atttatatca agtccttctc gaccagcctt
    15481 tttcctgccc ttcgtattac agcactcatt cttccaaatg ctatcaaaga agcatttgtg
    15541 gcctacaaaa atatcctaga ctacgacagc aacctcatta tgcaaaaggc cctgtcactc
    15601 tatattgaca gtcaattgtt tgaaaaaaat cgtttggctc gcttgaccaa tcatgaatct
    15661 taccaaaaac aaatcgagga aaggataact aaaacacctt gtccccttcc tcattatccc
    15721 ctacacgatg gtttattgct agacctgaga cagtatccta aaatcgccag tctcaaacac
    15781 agtcaactgg gcttggactt ctttgaagag gcctatttaa gcacctgtcc ttatcaattt
    15841 gccaaggtgt ccctagacaa tctggaaaat gttttaaact atttaaaagc agaattggaa
    15901 tgacatccaa ctctgctttt tcttattctt caacttcagt tagacttgct gctttttcaa
    15961 gatagctttg gtaagttcct tcattcttaa gtttttggat gaccttgtct accacttctt
    16021 tcaaatcatc actatttttt ctaagagcaa cggcattggc gtcgccgtcc ttcatcttca
    16081 agctgacagt tgcgacagct aagccagcgt ttttagcagc ataactaagt gcaacaggct
    16141 catccatatg aacagcatct acttttccag cctgcaattc attgactgct tcacccatat
    16201 tagttaggga agttaattga gcttttggca attgttcctt gaccattgat tctggaacag
    16261 tccctttttg ggctgcaata ttagcacttt ctaggctagt taaatccttg tatttttcca
    16321 catcagcctt acgaaccaag aaactaatct tgttttcata gtatgggatt gaaaaatcaa
    16381 agacttcttt tctctcgtca gtagcactaa ttcctgcaac tgctaggtca gccttaccag
    16441 tttgaagact ggtcaaaaca ttgtcaaaac tcatgcttga gatttccaac ttaaccccaa
    16501 gttcatcagc gatagcctga gccatgtcga tgtctgcacc gactacctgg ttctttccat
    16561 caaccaatga ttgaaattca aagggtgcat agtcaggact ggttgccaca actaatttcc
    16621 ccttttgctt aatggcctca acagctgact gagaaccatt agaacctgac tggcaagcta
    16681 ctaagaagaa acttgcaaga aaattacata aaacaaatat ccattttttt gattt
//
LOCUS       DAVLXF010000037        16318 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLXF010000037 DAVLXF010000000
VERSION     DAVLXF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16318
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.037"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            56..373
                     /locus_tag="VYO12_001167"
     CDS             56..373
                     /locus_tag="VYO12_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1587789.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FEF"
     gene            612..2171
                     /locus_tag="VYO12_001168"
     CDS             612..2171
                     /locus_tag="VYO12_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1587790.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLSKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIHSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(2215..3114)
                     /gene="htpX"
                     /locus_tag="VYO12_001169"
     CDS             complement(2215..3114)
                     /gene="htpX"
                     /locus_tag="VYO12_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1587791.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(3116..3676)
                     /locus_tag="VYO12_001170"
     CDS             complement(3116..3676)
                     /locus_tag="VYO12_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1587792.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            3770..4483
                     /gene="rsmG"
                     /locus_tag="VYO12_001171"
     CDS             3770..4483
                     /gene="rsmG"
                     /locus_tag="VYO12_001171"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1587793.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            4737..6020
                     /locus_tag="VYO12_001172"
     CDS             4737..6020
                     /locus_tag="VYO12_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1587794.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMLATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6215..7786)
                     /gene="ffh"
                     /locus_tag="VYO12_001173"
     CDS             complement(6215..7786)
                     /gene="ffh"
                     /locus_tag="VYO12_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1587795.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKVLAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(7798..8130)
                     /locus_tag="VYO12_001174"
     CDS             complement(7798..8130)
                     /locus_tag="VYO12_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1587796.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(8221..8598)
                     /locus_tag="VYO12_001175"
     CDS             complement(8221..8598)
                     /locus_tag="VYO12_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1587797.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            8670..9974
                     /locus_tag="VYO12_001176"
     CDS             8670..9974
                     /locus_tag="VYO12_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1587798.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            9987..10793
                     /gene="yidA"
                     /locus_tag="VYO12_001177"
     CDS             9987..10793
                     /gene="yidA"
                     /locus_tag="VYO12_001177"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1587799.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETDTGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTLVL"
     gene            complement(10848..10919)
                     /locus_tag="VYO12_001178"
     tRNA            complement(10848..10919)
                     /locus_tag="VYO12_001178"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10884..10886),aa:Arg,seq:cct)
     gene            complement(10961..11308)
                     /gene="rplS"
                     /locus_tag="VYO12_001179"
     CDS             complement(10961..11308)
                     /gene="rplS"
                     /locus_tag="VYO12_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1587800.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(11427..11756)
                     /gene="crcB"
                     /locus_tag="VYO12_001180"
     CDS             complement(11427..11756)
                     /gene="crcB"
                     /locus_tag="VYO12_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1587801.1"
                     /translation="MVIVYLAIACGFGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(11750..12124)
                     /gene="crcB"
                     /locus_tag="VYO12_001181"
     CDS             complement(11750..12124)
                     /gene="crcB"
                     /locus_tag="VYO12_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1587802.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(12121..12387)
                     /locus_tag="VYO12_001182"
     CDS             complement(12121..12387)
                     /locus_tag="VYO12_001182"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1587803.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(12503..12946)
                     /locus_tag="VYO12_001183"
     CDS             complement(12503..12946)
                     /locus_tag="VYO12_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1587804.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            13050..13985
                     /locus_tag="VYO12_001184"
     CDS             13050..13985
                     /locus_tag="VYO12_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1587805.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            14039..14323
                     /locus_tag="VYO12_001185"
     CDS             14039..14323
                     /locus_tag="VYO12_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1587806.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(14380..14958)
                     /locus_tag="VYO12_001186"
     CDS             complement(14380..14958)
                     /locus_tag="VYO12_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEV1587807.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKT
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSIAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(14933..15946)
                     /locus_tag="VYO12_001187"
                     /pseudo
     CDS             complement(14933..15946)
                     /locus_tag="VYO12_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            complement(15949..16318)
                     /locus_tag="VYO12_001188"
                     /pseudo
     CDS             complement(15949..16318)
                     /locus_tag="VYO12_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         4899 a         3682 c         3033 g         4704 t
ORIGIN      
        1 ttttttagtg ctatactata gggaaagagt acatcagatc aaggaggatg ctcacatgga
       61 agacaagaaa ctcattcaac tcctatccaa gttaaataaa agctaccaaa actgtaaaca
      121 gggtacggca gatgatattc gactacaaga gctgctaaac actactatgc aagagctcaa
      181 aaaaacggaa cagttgaaca acagtatctt aattgatctt gagaaatttt accaacctac
      241 cagtcttctg attggactgg gtagcctaaa actaaacgat caagcacgca ctgcttggcg
      301 aaactatgat aaattccatt acgagcatgt caaacacgta ctaagtctct atggacctgt
      361 ttttgaattt tagagcatag aatttccagt tttctgttga caaaatttcc ttaaaggtat
      421 aatataaaga tactaatact cggaggtaag ggagacatga acaactaagt ctatcaaata
      481 aagaaccttt atttagtaga tcttgttttt gtctcttttt gtgtgctctt ttatgctctt
      541 tttctggcat gttaatagag tttttttgac atagactttg ggctctacta ggtaaagtag
      601 agctttttgt tatgcactat gaacattcta gaaagggaaa tcatatgata aaaatcaatc
      661 atctaaccat cacacaaaac aaagatttac gagatcttgt atctgaccta accatgacca
      721 tccaagacgg ggaaaaggtt gctattattg gtgaagaagg aaatggcaaa tcaaccttac
      781 ttaaaatttt aatgggggaa gctttgtctg atttcactat caagggaaac atccaatctg
      841 actatcagtc actggcctac attcctcaaa aacttcccga ggagctgaaa aagaaaactt
      901 tacacgacta cttcttttta gattctattg atttagacta cagtatcctc tatcgtttgg
      961 cggaggaatt gcattttgat agcaatcgtt tcgcaagtga ccaagagatt ggcaatctat
     1021 cagggggaga agctttgaaa attcagctta tccatgagtt agccaaaccc tttgagattc
     1081 tatttttaga tgaaccttca aatgacctag accttgagac agttgattgg ctaaaaggcc
     1141 agattcaaaa gaccaggcaa accgttattt tcatttccca tgatgaagac tttctttctg
     1201 aaacggcaga cactattgtt cacttgcgac tggtcaaaca ccgtaaagaa gcggaaacgc
     1261 tagtagagca tttagactat gatagctata gtgagcagag aaaggctaat tttgccaaac
     1321 aaagtcagca agctgctaac aaccaaagag cctacgataa aaccatggaa aaacatcgga
     1381 gagttaagca aaatgtagaa actgcgcttc gagctaccaa agatagtact gccggtcgcc
     1441 tattgtctaa aaagatgaaa actgtcctct cacaagaaaa acgctacgaa aaggcagctc
     1501 agtccatgac tcaaaaacca cttgaagagg aacaaatcca acttttcttt tcagacatcc
     1561 aaccattacc agcttctaaa gtcttagtcc aactggaaaa agaaaatttg tccattgacg
     1621 accgagtttt ggttcaaaaa ctacaactaa ctgtccgtgg gcaagaaaaa atcggtatta
     1681 tcgggccaaa tggtgttggg aaatcaactc tgttagccaa attaaagcaa cttctgaatg
     1741 ataaaagaga gatttccctt ggttttatgc cacaagatta ccacaaaaaa ctacaattgg
     1801 atttatcacc aatagcttat atcagcaaaa ctggggaaaa agaggaactt cagaaaatcc
     1861 actctcacct agccagtctc aatttcagtt atccagaaat gcagcatcaa attcgctcct
     1921 tatctggcgg acaacaggga aaactcctgc ttttggatct agtcctgcgc aaaccaaact
     1981 ttctcctgct ggatgaaccc acacgaaact tttctcccac ttctcaaccc caaatcagaa
     2041 aactctttgc aacctatcca ggcggtctca tcactgtttc gcatgaccgt cgtttcttaa
     2101 aagaagtctg ctcgatcatc tatcgcatga cagaacacgg tttgaagcta gttaatttag
     2161 aagatttata aatttgcaac atagcaaaaa tccagagacg acctctggat tcttttacat
     2221 ctgttttaaa cgttcaatcc gttctgagat aggtgggtgg gtataaaaga gtttttggaa
     2281 ccccccacct ttcttgggat cattgatata aagagcactg ctagcatcat cgacgtggcg
     2341 actcatcggt ttgctattgt ccaacttatc tagggcatta atcattccct ggggattgcg
     2401 agtcagctca acactagatg catctgccag aaattccctc tgacgagaaa tagcgagctg
     2461 aaccaaggtt gcagcgagag gtgccagtac aatagctagt agggaaacca ctagcataat
     2521 gatttcaaga ccatttccat ctcggtcatc atcacttcgt ctgcgacctg ctccacccca
     2581 ccacatcata cgacctgcca taccagaaag catggtgata gcactagcaa gggcaactgc
     2641 aatagtcgaa atacggatat cataattacg aatatgactg acttcatgtc ccataacagc
     2701 ttctagttct tcacgattca tgatagctag tagacctgaa gtcgcagcaa cagccgcatt
     2761 ttgaggatta gaacctgtcg caaaggcatt taaggctgga tcatcaatga tgaaaacacg
     2821 gggcatagga atctgagcga ccagagccat atcttccact acatggtaga ggtctggtgc
     2881 cgtttgctca tccacctcac gcgctccatt catggacatg acaatctctg tcgattgaaa
     2941 aatcatagac aaagcgtaga taaagccgat aatcagtgcg ataaccaaac caccaagtcc
     3001 agatcttata aagagataac caaccgcata accaacaaga gctaagagta ggaaaaatac
     3061 cagcaacaaa atccaggttt ttcgtttatt gcttgcaatt tgatcaaaca acatcttagt
     3121 cacctaaacc gctaaaatca actttaggaa ccgacttttc ctcttcaggt gtttgaagga
     3181 aatctgccgc tttaaatcca aacattccag cgataatatt gctcgggaaa gtttctaatt
     3241 ttacattgta gttgctgaca acactgttat agagttgacg agagtaagaa attttatttt
     3301 ctgtgtttgt caactcctct tgcaatttaa caaagttagc actagctttc aaatctggat
     3361 agctttctgc aactgcaaaa atacctgaaa cctgacgagt gagggcatca ctggctttca
     3421 tagcttctgc tggtgaagtc gctgccgcca cttggttacg tagttctgcc accttttcaa
     3481 gggtagaacc ttcatatttg gcataacctt ttacagtctc aatcaagttc ggcaagaggt
     3541 catttcgacg tttcaactga acatcaatct gactccaagc ctccttggtt tgcatacgat
     3601 ttttaaccaa accgttatag ctaacaatca caaaaataac aataagagcg ataactccaa
     3661 gaataatcca agtcataata taagtccttt ctgcttttag attagtacca gtatatcaaa
     3721 ttttctatga ttgtggtaaa ataagatgat actaaagaag gaaataacta tgaaaccaaa
     3781 aacattttac aacttgcttg ccgagcagaa tcttccactt tcggaccagc aaaaagaaca
     3841 atttgaacgt tattttgagc tcttggtcga gtggaatgag aagattaatt tgacggcgat
     3901 tacggacaag gaagaagttt atctcaaaca tttttacgat tcgattgcac ccattcttca
     3961 aggtttgatt cccaatgaaa ctatcaaact tcttgatatc ggggctgggg caggatttcc
     4021 tagtctacca atgaaaattc tctatccgga gttagatgtg accattattg attcactcaa
     4081 taagcgcatc aacttcctac aactcttggc tcaagaactg gatttgaacg gagttcattt
     4141 ctaccacgga cgtgccgaag attttgccca agacaagaac ttccgtgctc aatatgattt
     4201 tgtaacagct cgtgcggttg cccgtatgca ggtcctatct gaattgacca ttccctacct
     4261 taaggttggt ggaaaactat tggcactcaa ggccagcaat gcgcctgagg aattattaga
     4321 agctaagaat gccctcaatc tcctttttag taaggtcgaa gacaatctca gctacgccct
     4381 accgaataga gatccgcgct atatcacagt ggtagaaaag aaaaaagaaa caccaaataa
     4441 atatccacgt aaggctggta tgccaaataa acgcccactt taaatttttt agtaaacaaa
     4501 tgtttacaaa atcagcctcg ctcttttatt tctaggctcg ggaaaaaatg atttacaaaa
     4561 tcattttttt ctgctatact atcctaagca aaggttttta atgtcatccc gtgaggtgac
     4621 gaagacgcag aaatatttaa aactctttaa aatctaaatt ttaaagaagt cttactctga
     4681 gggcctattg ctgtaaaata atgggctctt ttttgatgcc caaaagtgag gtttatatga
     4741 aacaagaatc aactgttgat ttgttactag acgttgacca acgtccttcg gctggaaaag
     4801 gaattctcct tagtttccaa cacgttttcg ccatgtttgg tgcgaccatc ttggtaccat
     4861 tgattttggg aatgcctgta tctgttgccc tttttgcttc aggtgttgga acactcatct
     4921 acatgcttgc tactggtttt aaagttccag tttatctagg ttcttcattc gcctttatca
     4981 cagctatgtc actggctatg aaagaaatgg ggggggatgt atctgctgct caaacagggg
     5041 ttatcttgac tggtttggtc tatgtccttg ttgctaccag catccgattt gtaggaacaa
     5101 aatggattga taaactcttg ccaccaatca ttatcggtcc tatgatcatc gttatcggtc
     5161 ttggacttgc aggttcagct gttaccaatg caggtcttgt agcagacgga aattggaaaa
     5221 atgctctggt agccgttgtt actttcctaa ttgctgcctt tatcaataca aaaggaaaag
     5281 gcttcctacg aatcattcca ttcctctttg ccattatcgg tggttacctc ttcgcactaa
     5341 ctcttggttt ggttgacttt acgcctgtcc tagaagctaa ctggtttgaa attcctggtt
     5401 tctacttgcc atttagcaca ggtggtgcct ttaaagagta caatctttac tttggtccag
     5461 aagccatcgc tatcttgcca atcgctatcg taacaatttc tgaacatatc ggagaccata
     5521 ctgttttggg tcaaatctgt ggtcgtcaat tcttaaaaga accaggtctt caccgtactc
     5581 ttcttggtga cggtatcgca acttctgttt ctgccttcct tggtggacca gccaatacaa
     5641 cttacggaga aaatacaggg gttatcggta tgactcgtat cgcttctgtc tcagttatcc
     5701 gtaacgctgc cttcatcgcg attgccctca gcttccttgg taaattcact gccttgattt
     5761 caactattcc aaacgctgta cttggtggta tgtcaatcct tctctatggg gttatcgcca
     5821 gcaatggttt gaaagtcttg attaaagaac gtgttgattt cgctcaaatg cgaaacctca
     5881 tcatcgcaag tgctatgttg gttcttggac ttggaggagc tatccttaaa cttggtccag
     5941 ttacactttc aggtactgcc ctttcagcca tgacaggaat catcttgaac ttgatcttgc
     6001 catacgaaaa taaagactaa gagtctaaat acacctaatc cactcagaca gctgagtgga
     6061 tttttcgtat accataataa aagtgtctta acaaaattat taaaatcaaa aaacgtataa
     6121 tatcagatat tctaaaacct tgatactgta cgttttatca tagaaatttt tactttattt
     6181 tctcatcaaa tgagatttgc atcaatctct tgtcttactt gcgtttcttc ttcgctttct
     6241 tcattttgtt agccatacgt ttcatggact gtttcatggc aaattcacca attttacctt
     6301 tcaaaccgcc accaaacatc tggctcatat ctggcattcc tgctcctccg agagctgata
     6361 agtcaggcat accgccttgt cccatcattc cttcaagggc agacatatcc attcctccca
     6421 tatttggcat atttttagga aggttatttg gattaatccc catttgcttc atcattttat
     6481 tcatatcccc agacataaca ccctgcatga gctgtttagc ctggttaaag tccttgatga
     6541 atttattgac ttcgacgaat gtatttccag aaccagcagc aatacgacgg cgacggcttg
     6601 gatttaacaa atctgggttt tcacgctctt caggtgtcat cgaagacaca atggcacgtt
     6661 tacgagcaat ctggcgttca tccaccttca tgttttgaag ggctggattg ttggccatac
     6721 ctggaatcat cttgagcaag tcttccatcg gccccatatt ttgcacctga tctaattgat
     6781 cgatgaaatc attaaaatca aaggtgtttt cgcgcatctt ctcagccatt tcaagggctt
     6841 tttgttcatc gtattcctga gaagctttct caatcaaagt gagcatatcc cccatgccaa
     6901 ggatacggct agacatacgg tctgggtgga aggtttcgat atctgtaatt ttttcacctg
     6961 taccagtgaa cttgattggt tttccagtga tgtgacgaac agacagagca gcaccaccac
     7021 gagtatcacc atcaatcttg gtaaggatga ccccagtcac ttccaactga gcattaaact
     7081 cacgcgcaac attggctgct tcctgaccaa tcatagcatc aacgacaagc aagatttcat
     7141 ttggttgagc caatactttc acatcacgaa gctcattcat gaggagctca tcaatctgca
     7201 aacgacccgc agtatcaatc aagacatagt cgttatgatt agtttgggct tgctccaaac
     7261 cttgacgtac aatctcaaca gctggtactt ctgttccaag tgcaaagaca ggcacatcaa
     7321 tctgttgtcc caaggtctta agctggtcaa tggcagctgg acgataaata tccgccgcaa
     7381 tcatcaaagg acgagcattt tcttctttct tgagtttgtt ggccaattta ccagcaaagg
     7441 ttgttttacc agccccttgt aaaccaacca tcatgatgat ggttggaatc ttaggtgact
     7501 tgataatttc tgccgtatca gaacctaaaa cggctgtcag ttcctcatca acgattttaa
     7561 taatctgttg cgcaggatta agcgtatcaa tgacctcatg cccgactgca cgctcacgaa
     7621 ctttcttgat aaagtccttt acaacaggca aggcaacgtc ggcctcgagc aaggccaagc
     7681 gaatttcttt ggttgcctct tggacatcag attcagagat ttttcctttt ttacgtagat
     7741 ttttaaagac gttctgcaaa cgttctgtta aactttcaaa tgccattttt cttcctctta
     7801 ttctctatta tcaatgcttg ttaaaatttc tatctgctcc tgcagaaagt catccttggg
     7861 atagcgctcc aaaatctgat caaaaatctg actgcggaca atatagtccg agtacatgtg
     7921 caatttcatc tcataatctt ccagaatctt ttctgttcgc ttgatattgt catagacagc
     7981 ctgacgactg acaccgaact cctcggcaat ttcagcaagg ctgtaatcat cagcgtagta
     8041 gagctcgata taattcattt gcttatctgt caaaagcgcc gcataaaatt caaagagcgc
     8101 attcatacga ttggtttttt cgatttccat aacttttatt ataccaaaaa ttagcctaat
     8161 ctaccacact aggaagccga tccaagaaga tagatagcta aatttgaaaa agacatgagc
     8221 ctagccccaa gtaatttcca attgatagct ggcaaaggga tgtccctctt gattttgtag
     8281 ttgataatct agttcaatct tttgcccatc cacttgataa cgattcgttt ggataataaa
     8341 ctcctgcata cccataggtg tgggaatata ggctaaacta tcactatcct ttagaaagcg
     8401 cataatggtc ttgggattgg aaaatcgact catcacaagt tcttgaccat gaaatttaat
     8461 cactactttt tccttttcct cattatagaa aagcaggtag ctataatctc ctttttcatg
     8521 cacttccaca tcataaagct ggtcaatcac ttccaactgc tcatcaaact gaattgtatt
     8581 tcgcatccga atcttcacat caggccctct ttcttgtctc ttgtcctact attttaccaa
     8641 aaagagcagg attttgctat aatggtcata tgaacgaaaa agtattccgt gaccctgttc
     8701 acaactacat ccatgtcaat aatcaaatca tctatgactt gattaataca aaagaatttc
     8761 aacgtttgcg ccggatcaaa caactgggaa cttccagtta taccttccac ggtggagagc
     8821 atagtcgctt ctctcactgt ctaggagtct atgaaattgc acgacgcatc acagagattt
     8881 tcgaagaaaa atatcctgag gaatggaatc ctgccgagtc tctcttgacc atgaccgctg
     8941 ctctcctaca cgaccttggg catggtgcct actcccatac ttttgaaaat ctctttgata
     9001 cagaccatga agccattact caggagatta ttcaaaatcc tgagacagag attcaccaag
     9061 tcctgctaca agtggcacct gatttcccag aaaaggtggc cagtgtcatt gaccatacct
     9121 atcctaataa gcaggtcgtg cagctcattt ctagccagat tgatgcggac cgcatggact
     9181 atctcttgcg tgactcctat tttacaggag catcctatgg ggaatttgac ctgactcgaa
     9241 tcctccgagt cattcgtcct atcgaaaatg gtatcgcctt tcagcgcaat ggcatgcacg
     9301 ccatcgaaga ctacgtcctc agtcgctacc agatgtacat gcaggtttat ttccaccccg
     9361 caacacgcgc catggaagtt ctcctacaga atcttctcaa acgcgccaag gaactctatc
     9421 ctgaggacaa ggatttcttt gcccgaactt ctccacacct cctgcctttc ttcgaaaaaa
     9481 atgtgacctt gactgactat ctggctctgg atgatggcgt gatgaatacc tacttccagc
     9541 tttggatgac cagtcctgac aagattcttg cagatttatc gcatcgcttt gtcaaccgca
     9601 aggtctttaa atccattacc ttttcacaag aggaccaaga tcaacttact agcatgagaa
     9661 aattggttga ggatatcggc tttgatcccg actactacac tgccattcat aagaactttg
     9721 acctccctta tgatatctat cgtcccgaat ctgaaaaccc acggacacag attgagattt
     9781 tacaaaaaaa tggagaactg gccgaactct ctagcctgtc tcctatcgtc caatcccttg
     9841 ctggcagtcg ccacggagat aatcgctttt attttccaaa agaaatgttg gaccaaaaca
     9901 gcatctttgc aagcattacc cagcaatttt tacacttgat tgagaacgat cattttaccc
     9961 caaataaaaa ctagaagagg aattttatga gtattaaact aattgccgtc gatattgacg
    10021 gaaccctagt caacagccaa aaggaaatca ctcctgaagt cttttctgcc atccaagatg
    10081 ccaaagaagc tggtgtcaaa gtcgtgattg caactggccg ccccatcgca ggtgttgcca
    10141 aacttctgga cgacttgcag ttgagagacc aaggtgacta tgtggtgacc ttcaatggtg
    10201 cccttgtcca agagactgat actggccatg agattatcag cgaatccttg acctatgagg
    10261 attatctgga tatggaattt ctcagtcgca agcttggtgt tcacatgcat gctattacta
    10321 aggacggtat ctataccgcc aatcgcaata tcggaacata cactgtgcac gaatcaaccc
    10381 tcgtcagcat gcctatcttc taccgcactc ctgaagaaat ggctggcaag gaaattgtca
    10441 agtgtatgtt catcgatgaa ccagaaatcc tcgatgctgc gattgaaaaa ataccagccg
    10501 aattttacga gcactactct atcaacaaat ctgctccttt ctacctcgaa ctccttaaaa
    10561 agaatgtaga caagggttca gctattactc acttagctga aaaactagga ttaaccaaag
    10621 atgaaaccat ggctatcgga gacgaagaaa atgaccgtgc catgctggaa gtcgttggca
    10681 accctgtagt catggaaaac ggaaatccag aaatcaaaaa aatcgccaaa tacatcacca
    10741 aaacaaatga cgaatccggc gttgcccatg ctattcgtac attggtactg taaaaggaaa
    10801 gaatagataa aaaccgctca gctgagcggt ttttgatgtc taattactgc cccctgcagg
    10861 aatcgaacct gcaactactc cttaggaggg agttgttata tccattgaac taagggagct
    10921 agataaaaac tctgctgaaa agcagagttt tttagtcgaa ttaacgacgg atttctttga
    10981 tacgagctgc tttaccttga agagcacgca agtagtacaa tttcgcacga cgtactttac
    11041 cgtaacgaac aacttcgatt ttttcaacac gtggagtgtg gattgggaag atacgctcaa
    11101 cacctacacc gttagagatt ttacgaactg tgtagttttc tgagattcca gcacctttac
    11161 gtgcgataac aacaccttca aaaatctgga tacgttcacg gttaccttcg acaactttcg
    11221 cgtgtacacg aacagtgtca ccaggacgga atgatgggat atctgtacga agttgacctt
    11281 cagtcaagct ttggattaat ggattcattt tattctccta tctttgtcaa tcttgaggaa
    11341 tcttcctcag cggataaact gtatttttgt gcgtccatta cacacaagat atagtttacc
    11401 aaatttctaa aaaaaagtaa agaaatttat agcaaaattc ctaaaaaaat cgcaaccaaa
    11461 ccacctatgt aagtcaaagt caggtaagaa taaaagacct tcttatcact tagcagtctt
    11521 tgaagttcgt cattcaaggt cgaaaaagtt gttaaaccgc cacaaaatcc tgttgctaga
    11581 atagcatata cttccttaga ctctacatga ttgtagaata ctccaattaa aaaacaacct
    11641 aaaagattag ctatgagcgt tccgagtggc aatttagaag cttgattata gcgagaaaag
    11701 aaataccgta cgagtgctcc gaaaccacaa gcaattgcaa gatagacgat taccatttct
    11761 tcctccctag aaaataagct aagagtaggc ctccaccgat gctcaaaagc aaatacagga
    11821 ctaaactaag ataacgccct gtatcaagca gcttcacagt atcaagcatt aggctggaaa
    11881 aagttgttaa acctccgcaa aaacccgtcc ccagtgctaa aatcaagccc ttactagtcc
    11941 ccttatagac cagatagccc ttgacaagaa aaatcaagca gaaaattccc agatagttga
    12001 caaagagagt gccccaagga aagtctggac tggctggtaa ccaggtggaa actagatatc
    12061 ggacaagtcc gcccaacata gcagctagaa aaattcctag cggataaaat tgttcttttt
    12121 tcatttgata ttttgatcct gataatcacg cgaacgtttg agtatgtccg aaaaagtcgc
    12181 gacaatagtc tcctgatagc gcttgtcctc tacacgactc ctaacctttt caaaaataac
    12241 ttcttctctc ttagtatcta aaatcggttt acctgaagct ttcttataag caaccacccc
    12301 ctcaactaaa tgcattcgtt cttctaacag tttgacaatt tggtcgtcga tttgatcgat
    12361 ttcttgacga ataatatcta aatccataca gtctcctcct ttatttgaat tattgtatca
    12421 aaaagccatc aattaggcta gtaaaatccc aactctcgat aagaaaaatg cactgattga
    12481 ttacatcagc gcacttttat gtttatccta ctttagcagc caattcttct gcgaattgtt
    12541 ccaagcgttc aatatcttct tcctcggcag aaagatcaac tttaacacac tctgaacctt
    12601 tttctgctcc tgttgacaca aagacacggt caaagtcatc aacagcctta cagaattcat
    12661 cgtagaaggt atctcctgag ccgaccactc cgtagatttt gccattcaag ttgagatctg
    12721 ctaggtcttc gtagaagtcc atcatctcat ctggcaattc accatcacca taagtatagg
    12781 tcgcaacgat agcgatgtct gcttccaaga agtctgaagc gtcaacagtt gtacattcat
    12841 caacatcaac atccaagccc aagtcacgta atttgtctgc tacaatatct gcaatttctt
    12901 cggtattacc ggtcatactg gcaaatacaa tttttgctaa tgccatatcg tcctcctcaa
    12961 tttatctttc tccattatat cacatctttt tcattttaaa aataaaaatt cttgtgctac
    13021 aatgaaatga gaaagaattg aggttattta tggagatttg ccaacaaatt ttagaaaaaa
    13081 ttaaagaata cgacaccatt atcattcatc gtcatatgaa accagaccct gatgccttgg
    13141 gaagtcaggt gggattgaaa gccttgctgg aacatcattt cccagaaaaa accatcaaag
    13201 ccgtcggttt tgatgaacca actcttactt ggatggctga gatggatctt gttgaagata
    13261 gagcctacca aggcgcactt gtcatcgtct gtgatacagc taatactgct cgtatcgatg
    13321 ataagcgcta tagtcaaggt gattttctca ttaagattga ccaccatcca aatgatgatg
    13381 tatacggtga cctgtcttgg gtcgatacta gttcaagtag cgctagcgag atgattaccc
    13441 tatttgccca aacaacccaa ctagccttgg cagatcgcga tgctgagttg ctctttgcag
    13501 gaattgtcgg tgatacaggt cgcttcctct acccttctac cactgcacgg actcttcgcc
    13561 tggctgctta tttgagagaa cataactttg actttgcggc tctcactcgc aaaatggaca
    13621 ctatgagcta caaaattgct aaactgcaag gctacatcta cgaccatctg gaagtggatg
    13681 aaaatggtgc tgctcgcgtt atcctgagtc agaaaatctt gaaacaatac aatataaccg
    13741 atgctgaaac tgcggccatt gtaggtgcac ctggacgcat tgacagagtg agtctctggg
    13801 gaatttttgt cgaacaggct gatggccact accgagttcg cttacgcagt aaagtccatc
    13861 ctatcaatga aattgccaag gagcatgatg gtggaggcca ccctctagca agtggtgcta
    13921 attcctatag cctagaagaa aacgaaatca tctaccaaaa gttaaaaaac ttgcttaaaa
    13981 actgataaaa tacttgccaa acttttcaga atctgataga ctagtatagt aacaatctat
    14041 ggctcgcaaa gagaccatgg cagaaaggaa atattgcaaa atgaaaaaag atatccatcc
    14101 agaatatcgc ccagttgtct tcatggacac aactactggt taccaattcc ttagcggttc
    14161 aacaaaacgc tctaacgaaa cagttgagtt cgaaggcgaa acttacccat tgatccgtgt
    14221 ggaaatttca tcagactcac acccattcta cactggacgt caaaagttca ctcaagcaga
    14281 tggacgcgtg gatcgtttca acaaaaaata cggtctcaaa taatgataag agaacagttt
    14341 tggctgttct tttttgtttc ttgaaatcaa ctgctgtttt catgttccag actcatctgt
    14401 aggttcgatt tccatgctac taggcaggaa ggaaatagct gtttcaacac gtccataatg
    14461 agctatacta ttgtcacgaa ccacactttc attgatggtc caagtggaat tcattttctt
    14521 aaaagcttct cggacttttt ccaaatcttt ggaggcaatg gcctgctcta aggtttcaaa
    14581 acgaggactt agacggttta ccaatttttc cttttccgca tctaggtcaa caggattttg
    14641 ttctttttca aaagctaata gagctgagga aatttcggtg agtttggctt cagtaatatc
    14701 tccagataga gctaactttt ccttaacaat cttaccaacc tcagaatcct tattttctac
    14761 tctttcgaag tctgatgcca tcttcgtaac gagtttctgg gtctcagtct gaccaccatt
    14821 tttaaccgtt ttggatgcat ctgtgatttt cacaaagaaa aaactcaagc tttcttgggc
    14881 acctgctggg gaaagaatta aaaatgacaa agctaaaact agtaaccagc ttttagcctt
    14941 ccaataattt ctgaccaatg tagcctcctt tctccacacc accaaagcaa gtaaaaagtc
    15001 cactacctat gtgagtgatg tattcattca tcttatcagt agcacctagg ttagtttgaa
    15061 ccttaacaaa gttatcagga tccttctgga aagaaatgaa gagcaatccc gtatcaaact
    15121 gaccagtctt ttcatcaatg ccatctgagt aagaataaga acgacgtaaa agtggcttat
    15181 ccacttcttt ggccaaacaa acatgcgaat catcaggtaa gagactcaaa tccacttcat
    15241 caaactcatt tttcttacca aagggggcac cgctttcctt gtaacgacca aaggtatttt
    15301 ctcgttcttc gagactagtg cggtcccagg tctctaaaaa catctgaatc cgacgaactg
    15361 ccatataaga accattttcc atccagtcct tactatcagc ccagataacg cggtcaaagt
    15421 cttgctcctt ggttggattt gctgttccat ccttaaaacc aaagaggttc cacggagtct
    15481 ccattcgatc cccgatagct gcaaatccag actgactcca acgaaggggc accgcatttc
    15541 tccccttacg gatgagattg cgaatagcgt gaaaggcaat ttgctcatca tctgcacaag
    15601 catgaatcac aatatctcca ccagtatatt tttcacgtaa ttgctcctta ggaaatagag
    15661 gtaaatccct gaacagtcga gggcgcttgt tttccaagtt catccttttc aagaaacttg
    15721 cagatacacc aaaagtcagc gtcaaacgat gaggattgag cccaactgtc tcaccagtat
    15781 cgctcggagg aaagagagca ttctgtccat cttttttaac caattccccc tcgaccaact
    15841 tggcactata atccgtccaa tccttgaata gctggataat tttatctcta tccgtcgtat
    15901 gcaggtccaa gacaacaaaa tagatattct tttgcatggg cgtactattt ggtacatcaa
    15961 gcgaacctta tgaaaaggtc tactggtttt aaagcgcttt cttttcttct tgaattgaga
    16021 ttttattttt agacgcgaca atggtttgag cgtctgcttt cctaggatgt tctggacgtg
    16081 gtgtaatatt tcgccaacca tggcgcttca acagttgata gaaggcatca cgtgtgtagg
    16141 aacgacctaa ctccttttta taagcctgaa ataaggcatc aattgtaaca aattctcctg
    16201 cctctgtagc cttcaaatga cgggcaagaa aggctttctc ttcctcaact gtcatatatg
    16261 catggttacg accaccacgt gtttcttgaa ggagagagtc gagtccgaac tcctcata
//
LOCUS       DAVLXF010000038        16037 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLXF010000038 DAVLXF010000000
VERSION     DAVLXF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.038"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            372..755
                     /locus_tag="VYO12_001189"
     CDS             372..755
                     /locus_tag="VYO12_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1587808.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            807..1496
                     /locus_tag="VYO12_001190"
     CDS             807..1496
                     /locus_tag="VYO12_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1587809.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1538..1771
                     /locus_tag="VYO12_001191"
     CDS             1538..1771
                     /locus_tag="VYO12_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587810.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFLGAGYVVGFWLLILNENQRAN"
     gene            1922..2533
                     /locus_tag="VYO12_001192"
     CDS             1922..2533
                     /locus_tag="VYO12_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1587811.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFVPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYKSWPLLYYL"
     gene            2694..3488
                     /locus_tag="VYO12_001193"
     CDS             2694..3488
                     /locus_tag="VYO12_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1587812.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETVPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3485..4120
                     /gene="trmB"
                     /locus_tag="VYO12_001194"
     CDS             3485..4120
                     /gene="trmB"
                     /locus_tag="VYO12_001194"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1587813.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4246..4725
                     /gene="rimP"
                     /locus_tag="VYO12_001195"
     CDS             4246..4725
                     /gene="rimP"
                     /locus_tag="VYO12_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1587814.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4769..5905
                     /gene="nusA"
                     /locus_tag="VYO12_001196"
     CDS             4769..5905
                     /gene="nusA"
                     /locus_tag="VYO12_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1587815.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5927..6220
                     /locus_tag="VYO12_001197"
     CDS             5927..6220
                     /locus_tag="VYO12_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1587816.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6213..6512
                     /locus_tag="VYO12_001198"
     CDS             6213..6512
                     /locus_tag="VYO12_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1587817.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6529..9321
                     /gene="infB"
                     /locus_tag="VYO12_001199"
     CDS             6529..9321
                     /gene="infB"
                     /locus_tag="VYO12_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1587818.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9572..9922
                     /gene="rbfA"
                     /locus_tag="VYO12_001200"
     CDS             9572..9922
                     /gene="rbfA"
                     /locus_tag="VYO12_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1587819.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            10201..10980
                     /locus_tag="VYO12_001201"
     CDS             10201..10980
                     /locus_tag="VYO12_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587820.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQDPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            10982..11164
                     /locus_tag="VYO12_001202"
     CDS             10982..11164
                     /locus_tag="VYO12_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587821.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(11507..11581)
                     /locus_tag="VYO12_001203"
                     /pseudo
     CDS             complement(11507..11581)
                     /locus_tag="VYO12_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11612..11845
                     /locus_tag="VYO12_001204"
     CDS             11612..11845
                     /locus_tag="VYO12_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1587822.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            11845..13179
                     /locus_tag="VYO12_001205"
     CDS             11845..13179
                     /locus_tag="VYO12_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1587823.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            13189..13443
                     /locus_tag="VYO12_001206"
     CDS             13189..13443
                     /locus_tag="VYO12_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1587824.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            13539..13934
                     /locus_tag="VYO12_001207"
     CDS             13539..13934
                     /locus_tag="VYO12_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1587825.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            14347..14796
                     /locus_tag="VYO12_001208"
                     /pseudo
     CDS             14347..14796
                     /locus_tag="VYO12_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
     gene            14783..15340
                     /locus_tag="VYO12_001209"
     CDS             14783..15340
                     /locus_tag="VYO12_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1587826.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAACALIDLAFKELGLHKIELTCFGYNLQSQRVSEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            15342..>16037
                     /locus_tag="VYO12_001210"
     CDS             15342..>16037
                     /locus_tag="VYO12_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1587827.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEY"
BASE COUNT         4890 a         2884 c         3644 g         4619 t
ORIGIN      
        1 aaccccgtta ctgttcattt taaatattat tggcctgtct tgcttttcag tgctaacata
       61 cattaacata aaagagatac tcttaaatat aagtaaatga tgatttggtt agtcctagaa
      121 ccaatcaact tatgtttatc ggcttggcag attttatgtt tgtcatttgt ttgtatagag
      181 gtatttcaga aacagaattc tatcaacaac taatcgctta tataggtgta ttctctgctt
      241 gtttatcacg cttctgctca tgtggggctt gaagtattat aaaaagtagc tttctatcct
      301 tttctgacag gatatttgag tagaaggctc tattaaattg ttagtgaaat aaatgaatgg
      361 aggtatttaa tatgaaatac agattatttt ttgttatttt cttgagtagt gtgttggata
      421 ttcttttagg gacattttta caaatctcta tcgtatctat tgggtggctt gttctttaca
      481 gtggtttgtt tgaagcagga gttttccttc ttgctaataa aggggtggcg gtaaaaatca
      541 aggaagtaga tattcgaaat cgctttaaat ttatttttgg aaaaacctta tggtttcaaa
      601 ttcttttgct catctttttg ataatcaaac tttatcttgg tttggatgcg aggttgattt
      661 tattctatgg acatattttc attgtcttta atgccttaat gtatctatta tctagtagtc
      721 aggttagcct taaaaaaaac aaactgtctt cttaatctta cagcagtaga gcctgacaag
      781 gtgaggtcat agtagggagg atttctatga aaaagtatca acttctattc aaaataagtg
      841 cagtcttctc ttacttattt ttcgtatttg gtctttctca gctgacgctt atcgtccaaa
      901 actattggca attttcttct cagataggca atttattctg gattcaaaat atcttgagtt
      961 tactttttat tggagtcatg attgtggttc ttgttaagac aggccatggt tatctcttcc
     1021 gcattccaag aaaaaaatgg ctttggtatt cgattttgac agtattagtg gtagtgttac
     1081 agatctcttt taacgttcag acagctaaac atgttcagtc aactgcggaa ggttgggctg
     1141 tattgattgg ttatagtggg actaactttg cagagctagg tatttatata gccctgttct
     1201 ttctggttcc actgatggaa gaattgattt atagaggatt actgcaacat gctttcttta
     1261 agcattcgcg atttggtctt gatttgcttc ttccttctat tttatttgct ctccctcatt
     1321 tttcaagcct gcctagtctg ttagatatct tcgtctttgc aacagttgga atcatctttg
     1381 ctggtttgac ccgctatacc aagagcattt atccatccta tgcggtgcat gtgatcaata
     1441 atattgtagc gaccttcccg tttttgctca cttttctaca tagggtcttg gggtaaaaaa
     1501 acaaaaggct tgcttttcag ccatagagga ggtcatcatg tataaacact tatttttcct
     1561 agattccaaa actttagatc ggttgacacc ctatattcta gtcttggctt ctgacaccat
     1621 tgcttttaat gtttttgtgc taacctttgt atctgcggtg gtttttaatt tcctaaattc
     1681 catgctagct ttaatggcta tcttcttagg agctggctat gtggtcggat tttggttact
     1741 aatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgt acttgagtac
     1801 ggcaaggcga cgttgacgtg gtttgaattt gattttcgaa gagtataaaa tggtttgttt
     1861 tggaaaggct agaactaaag gatgacgtgt aggaagtctg tttgttgagg aggataattt
     1921 tatggagttt tttgataaat ttcatgcctt gtgttttgga tttttagtac taataattgt
     1981 cattacagtt ccttatacga ttaaccatgg ggattttttt caaaatgaat ctgcattgat
     2041 tattgtaagt cttcttgtaa cctcgctgag tgttgcttat gctagaaagt ttgaaatgat
     2101 ttcttttggg atgttaagca agaaacaact tttgcttttc attgcaatct ttcttctaag
     2161 tgtgcttgag acgctggttt atattcattt cttcgctgtt tcttctggct caggggtcca
     2221 acacttggcg gaagtcagca gaggaatttc cctgtctttg attttgacta cctcagtttt
     2281 tgtccccatc caggaggaac tcattttcag aggacttctt caaggtgcgg tttttgacaa
     2341 ttcttggtta gggcttgtgc taacttcctc tctcttttct ttcatgcatg gaccttctaa
     2401 tgtcccttcg tttatttttt atctacttgg gggcttgtta ctgggctttg cttataaaaa
     2461 gagtcaaaac ctatgggttt ctactctagt acatatgctt tacaaaagtt ggccactctt
     2521 atattattta taaaaattat gaaaaggtaa gcagaagacg gtgcttacct ttttcaagct
     2581 ataatgatac ccaagccaat ccagaggctt ttctttgaga atcgggttag agtggttgac
     2641 gaataggcca aaaactagta gaatagtaag gaaactttat acggaggaaa gaaatggatt
     2701 tgggtgataa tgagctaaca ctgactccca tacctgggaa aagtggcaag gcttatatgg
     2761 gtagctatcc tgatgggaag cgcatctttg taaaaatgaa cacctctcca atcctacctg
     2821 gtctagctag agaacaaatt gctccacaat tattatggag tcgtcgtttg gcagatgggc
     2881 gtgatatgtg tgctcaagaa tggttgacag gcaagatatt gaccccctat gatatgaatc
     2941 gtaagcaaat cgtcaatatt ttaacccgtc ttcatcgctc acgtccgttg atgacacaat
     3001 tgagtcgttt gggctatgcc atggaaacac ctgtagattt actacagtct tggcaggaaa
     3061 cggttccaga tgctttgcgt aaaaatcatt ttatcagtga agtgatggct gatttacgtc
     3121 agactattcc aggatttaga gaggaccatg cgaccattgt ccatggagat gtacgacata
     3181 gtaattggat tgagacagat agtggcttga tttatttagt agattgggat tcggttcgct
     3241 tgaccgatcg catgtttgat gtggcccata tgctctgcca ttatatttca gaacatcagt
     3301 ggaaggaatg gttgacctac tacggttaca agtacaatca aacggtatta agtaaattgt
     3361 attggtatgg tcaattgtct tatttgagtc agatttccaa gtattatatg aaccaagatt
     3421 tagaaaatgt caatcgggag attcatggtt tgcgtcattt ccgagacaag tatggaaaga
     3481 gaagatgaga gttagaaatc gtaaaggggc aacagaatta ctagaggcaa atccccagta
     3541 tgtggtcctc aatcccttgg aagccaaggc aaaatggcgg gacttgtttg gcaatgataa
     3601 tcccattcat gtggaagttg gaagtggaaa gggtgccttt gtttcaggta tggccaagca
     3661 aaaccctgac atcaactata tcgggattga tattcaaaag tctgttttga gctacgcttt
     3721 ggacaaggtg cttgaagctg gagtgtctaa catcaagctc ttgtgggtag atggttctga
     3781 cttgactgac tactttgaag acggtgagat tgatcgcttg tatctgaact tttcagatcc
     3841 atggccgaaa aaacgccatg aaaagcgtcg tttgacctac aagacctttt tggatacctt
     3901 caaacgtatc ttgcctgaaa atggagaaat tcatttcaag acggataacc gtggcttgtt
     3961 tgagtacagt ttagtgagct tttctcaata tggcatgaaa ctcaatggtg tctggctaga
     4021 tttgcatgcc agtgattttg aaggcaatgt catgacagaa tacgagcaaa aattctcaaa
     4081 caaggggcaa gttatctacc gagttgaggc agaattttaa gagatagcct aaaattaggc
     4141 tgtacaagtg cttttgcttt acataagttg gcaaacgtgc tatactgata gtaagaatat
     4201 gaaaagtgag gcggggaaat atcttcgcct cttgcttatg aggaggtgga cgcaatcgca
     4261 acaatcgtag aattagtcag agaagttgta gaacctgtca tagaagctcc ttttgaactc
     4321 gtggatatcg agtatggaaa gattggcagt gacatgattc tcagtatttt tgtagataaa
     4381 cccgaaggaa ttaccttgaa cgacacggca gacttgacag aaatgatcag tcctgtccta
     4441 gacaccatca agccagatcc cttcccagaa caatatttcc tagaaattac cagtccaggt
     4501 ttggaacgtc ctttgaaaac caaggatgcc gtcgctggag cggttggaaa atacatccat
     4561 gtcgggctct accaagccat cgataagcaa aaggtctttg aaggaacttt gttggccttc
     4621 gaagaggacg agttgactat ggaatatatg gacaagacgc gtaagaaaac cgtccaaatt
     4681 ccatacagtt tagtatcaaa agcacgttta gcagttaaat tatagaaaaa gaaaggatag
     4741 cttttgagga ttcaaaagtg aagaaaacat gagtaaagaa atgctagagg ccttccgcat
     4801 tttggaagaa gacaagggaa tcaaaaaaga agatatcatc gacgcagtag tagagtcgct
     4861 tcgttccgct tatcgcagac gctatggtca gtcagacagc gtagctattg acttcaacga
     4921 aaaaacaggt gactttacag tttatactgt ccgtgaagtt gttgatgaag tatttgatag
     4981 ccgtttggaa atcagcttga aagatgctct tgccattaat tcagcttatg aacttggaga
     5041 caaaatcaag tttgaagaag caccagctga gtttggtcgt gtagcagccc aatctgccaa
     5101 acaaaccatc atggaaaaaa tgcgcaagca aacacgtgcc atcacttaca atacttacaa
     5161 agaacatgag caagaaatca tgtctggtac agtagaacgc tttgacaacc gctttatcta
     5221 tgtcaacctt ggtagtatcg aagcccaatt gtcaaaacaa gaccaaattc ctggagaagt
     5281 ttttgcttct catgatcgta tcgaagttta tgtttacaag gttgaagaca accctcgtgg
     5341 tgtgaacgtc tttgttagcc gtagccatcc agaaatgatc aaacgtttga tggagcaaga
     5401 aattccagaa gtttatgatg gaactgttga aatcatgagc gtggctcgtg aagcaggtga
     5461 ccgtacgaag gttgctgttc gtagccacaa tccaaacgtg gatgctatcg gtacaatcgt
     5521 tggacgtggt ggtgctaata tcaagaagat tactagcaaa ttccacccag ctcgttacga
     5581 tgctaaaaat gaccgcatgg taccaatcga agaaaatatc gatgttatcg agtgggtagc
     5641 agatccagct gaatttatct acaatgccat cgctcctgct gaggttgacc aagttatctt
     5701 tgatgaaaac gacagcaaac gtgccttggt ggttgttcca gataacaagc tttctcttgc
     5761 cattggtcgt cgtggacaaa acgtgcgctt ggcggctcac ttgactggtt accgtatcga
     5821 tatcaagtct gctagcgaat ttgaagccat ggaagacgct gcttcagtag agttggaagt
     5881 agaaaacgat actgtagaag aataaaagct gctagaggag ggaaagatga aaacaagaaa
     5941 aatccctttg cgcaagtctg ttgtgtctaa cgaagtgatt gataagcgtg atttgctccg
     6001 cattgtcaag aacaaggaag gacaagtctt tattgatcct acgggcaagg ccaatggccg
     6061 cggcgcttat atcaaactag acaatgcaga agccctagag gcgaaaaaga agaaggtctt
     6121 taaccgcagc tttagcatgg aagtggaaga aagcttttat gacgagttga tcgcttatgt
     6181 ggatcacaaa gtgaaaagaa gagagttggg acttgaataa gcaaaagata agtaatctct
     6241 tggggcttgc tcagcgagca gggcgcatca tatcgggtga agaattggtg gtcaaggcca
     6301 ttcaagacgg caaggccaag ttggtctttc tagctcatga tgctggaccc aatctgacca
     6361 agaagattca agataaaagt cattattatc aagtagaaat tgtaaccgtg ttttcaacac
     6421 tggaattaag catagcagtc gggaaatcga gaaaggtttt ggctgtaaca gatgctggat
     6481 ttacaaagaa aatgaggtct cttatggaat agaagaggag gacatgattt gtctaagaaa
     6541 agattgtacg aaatcgcaaa agaacttgga aaagaaagta aagaagttgt agcgcgtgca
     6601 aaagagttag gcttggatgt gaaaagccac tcatcaagtg tggaagaagc tgtcgctgca
     6661 aaaattgctg ccagctttaa gcctgcagct gctccgaaag tagaagcaaa acctgcagca
     6721 ccaaaagtaa gtgcagaaaa gaaagccgaa aaatctgagc cagctaaacc agctgtagct
     6781 aaggaagagg caaaaccggc tgagccagtt gctccgaaaa cagaaaaagt agcagtgaaa
     6841 ccgcaaagtc gtaatttcaa ggctgagcgt gaagcccgtg ctaaagagca ggcagaacga
     6901 cgcaagcaaa ataagggcaa taaccgtgac caacaacaaa acggaaaccg tcagaaaaac
     6961 gacggccgta atggtggaaa acaaggtcaa agcaaccgcg acaatcgtcg ctttaatgac
     7021 caagctaaga agcagcaagg tcagcaaaaa cgtagaaatg agcgccgtca gcaagaggat
     7081 aaacgttcaa atcaagcggc tccacgtatt gactttaaag cccgtgcagc agccctaaaa
     7141 gcagagcaaa atgcagagta cgctcgttca agtgaggaac gcttcaagca gtatcaggct
     7201 gctaaagaag ccttggctca agctaacaaa cgcaaggaac cagaggaaat ctttgaagaa
     7261 gcggctaagt tagctgaaca agcacagcaa gttcaagcag tggttgaagt cgtccctgag
     7321 aaaaaagaac ctgcagtgga tacacgtcgt aaaaaacaag ctcgaccaga caaaaatcgt
     7381 gacgattatg atcatgaaga agatggtcct agaaaacaac aaaagaatcg aagtagtcaa
     7441 aatcaagtga gaaatcaaaa gaatagtaac tggaataaca acaaaaagaa caaaaaaggc
     7501 aataacaaga acaaccgtaa tcagactcca aaacctgtta cggagcgtaa attccatgaa
     7561 ttgccaacag aatttgaata tacagatggt atgaccgttg cggaaatcgc aaaacgtatc
     7621 aaacgtgaac cagctgaaat cgttaagaaa cttttcatga tgggtgtcat ggccacacaa
     7681 aaccaatcct tggatgggga aacaattgaa ctcctcatgg tggattacgg tatcgaagcc
     7741 aaacaaaagg ttgaagtgga taatgctgac atcgaacgtt tctttgtcga agatggttat
     7801 ctcaatgaag atgaattggt tgagcgtcca ccagttgtta ctatcatggg acacgttgac
     7861 cacggtaaaa caaccctttt ggatactctt cgtaactcac gtgttgcgac aggtgaagca
     7921 ggtggtatta ctcagcatat cggtgcctac caaatcgtgg aaaatggtaa gaagattacc
     7981 ttccttgata caccaggaca cgcggccttt acatcaatgc gtgcgcgtgg tgcttctgtt
     8041 accgatatta cgatcttggt cgtagcggca gatgacgggg ttatgcctca gactattgaa
     8101 gccatcaacc actcaaaagc agctaacgtt ccaatcatcg tagctattaa caagattgat
     8161 aaaccaggtg ctaacccaga acgcgttatc ggtgaattgg cagagcatgg tgtgatgtca
     8221 accgcttggg gtggagattc tgaatttgtt gaaatctcgg ctaaattcaa ccaaaatatc
     8281 gaagaattgt tggaaacagt ccttcttgtg gctgaaatcc aagaactcaa agcagaccca
     8341 acagttcgtg cgatcggtac ggttatcgaa gcgcgcttgg ataaaggaaa aggtgcggtc
     8401 gcaacccttc ttgtacaaca aggtaccttg aatgttcaag acccaatcgt tgtcggaaat
     8461 accttcggtc gtgtccgtgc tatgaccaac gaccttggtc gtcgtgttaa agttgctgga
     8521 ccatcaacac cagtctctat cacaggtttg aacgaagcac cgatggcggg tgaccacttt
     8581 gccgtttacg aggatgaaaa atctgcgcgt gcagcaggtg aagagcgtgc caaacgtgcc
     8641 ctcatgaaac aacgtcaagc tacccaacgt gttagccttg aaaacctctt tgataccctt
     8701 aaagctgggg aactcaaatc tgttaatgtt atcatcaagg ccgatgtaca aggttctgtt
     8761 gaagcccttt ctgcctcact tcaaaagatt gacgtggaag gtgtcaaagt gactatcgtc
     8821 cactcagcgg tcggtgctat caacgaatca gatgtgaccc ttgccgaagc ttcaaatgcc
     8881 tttatcgttg gtttcaacgt acgccctaca ccacaagctc gtcaacaagc agaagctgac
     8941 gatgtggaaa tccgtcttca cagcattatc tacaaggtta tcgaagagat ggaagaagct
     9001 atgaaaggga tgcttgatcc agaatttgaa gaaaaagtta ttggtgaagc ggttatccgt
     9061 gaaaccttca aggtgtctaa agtgggaact atcggtggat ttatggttat caacggtaag
     9121 gttgcccgtg actctaaagt ccgtgttatc cgtgatggtg tcgttatcta tgatggtgaa
     9181 ctcgcaagct tgaaacacta taaagacgac gttaaggaag ttacaaacgg ccgtgaaggt
     9241 ggattgatga tcgacggcta caatgatatt aagatggatg atgtgattga ggcgtatgtc
     9301 atggaagaaa tcaagagata agattttttg ctcctttctt aggtggtgag ggacgcaagc
     9361 aaaccgatgg tttcattgct tatttttgag cctagggtct caaaaatacc ctgtgatggg
     9421 actgataaat cagttccatc actttcacca cggcgaaaga agcagatgac ttcaaattga
     9481 acttcgtttc aatttaaact gaaaatcaag aagtttaaaa tagctaggtc tgctggccta
     9541 gcttttggtt caaagtagag aaaggaatat catggcaaat catttccgta cagatcgtgt
     9601 gggcatggaa atcaagcgtg aagtcaatga gattttgcaa aagaaagtcc gtgatccacg
     9661 tgtccaaggt gtgaccatca cagatgttca gatgctgggt gacttgtctg ttgccaaggt
     9721 ttattacacc attttgagta accttgcttc ggataaccaa aaagcccaaa tcgggcttga
     9781 aaaagcaact ggtaccatca aacgtgaact tggtcgcaat ttgaaattgt acaaaatccc
     9841 agatttgacc ttcgtcaaag acgagtccat cgagtatgga aacaagattg acgagatgct
     9901 acgcaatctg gataagaact aaagaagagg ggttgcccct cttttttggt ggaggaaaat
     9961 aggttgaatt tgaaatggaa aaatattctt ttataataga ttgaaactag aatagtacgc
    10021 ctctacttct aaaacattgt tagaaatcga tttgactgtc ctgatcgatt tgtcctgttc
    10081 ttgtttcatt ttactataaa aaaggggttc tgtatttttt aatgttatct aattagaaaa
    10141 tgcttttttt gtaggaaata taatatgata aggtgcaaaa aagaaataag gagtttgtat
    10201 atggctgaac aagacttagc tatgcaagta ttgcaacaag tggtgaaact acctgttgtt
    10261 aaggttgatc gttcgaaatt tttagtggat aagttttcca aagaattgga tccaaaagat
    10321 attcctacct tattggaaca agacccaacg actcttctat ctcaagaaat attagatcgt
    10381 gtagctaatg cttgtattcg ggacaatgta ttattagcga gtgggacttc tgttttggca
    10441 ggattacctg gagggcttgc tatggcaatt accattccag ctgatgtggc tcaattttat
    10501 gctttctctc tgaaattggc tcaagaatta ggttatattt atggttatga ggatctttgg
    10561 gcttcacgcg aggagttgag tgaagatgct caaaataccc tcttgcttta tctaggcgta
    10621 atgttagggg tgaatggaac cgctgctttg ctacgtgctg gtggtataac aattgccaaa
    10681 caggtaatga aaacagtgcc taataaagct ttaacaaaga cgctttggta ccctattttg
    10741 aaaaaagtct taaaaatatt tggtgtgaat cttaccaagg gagggctggc caaaggaata
    10801 gggaaattta ttcctatctt gggtggtatc atttcaggtg gtttaacctt tgcaactatg
    10861 aaaccaatgg gggaaagctt gcagaaagaa ttatccaagc tagtcaacta tagtgaagtt
    10921 caatatcaag aagatgttga aacaatccga aaagaggctg aaatcatcaa aggagagtaa
    10981 tatgaatcct atcaaagctt ttgctaaaat ttatggtaat tactttttga ccgtgcaagg
    11041 tgtaaaagtg atgaaaacga taaagaaagc tgaccatgtc gttgttggtc tgggcaaact
    11101 ttttattgca gataagctaa tggatacggc tcggtggctc attaagccag aggagagaga
    11161 atgaaatttt tttggtcttc ttgctattct ttttatcaaa ccgattattg ggattgtgaa
    11221 attcttttgg atgatcattt cttttgcagt tcaattgctg ttttacaaga tattggattg
    11281 gttctttaaa cttatctaga tggtaatcca agttgcagag aactagcagg aactccactg
    11341 ctagtttttt attctctttc catatggtat aatataagca gtaaaatcat tttatactct
    11401 tcgaaaatct cttcaaacca cgtcagcttc accttgcagt atatatgtta ctgacttcgt
    11461 cagttctatc cacaacctca aaacggtgtt ttgagctgac ttcgtcagtt ctatctacaa
    11521 cctcaaaaca ctgttttgag caacctgcgg ctagcttcct agtttgctct ttgattttca
    11581 ttgagtatta gaacatacaa tggaggtcgt catggacaat atcatcgatg tgtcaattcc
    11641 tgttgcagaa gtggtggaca agcatccaga agtcttggaa attctagtgg agttgggttt
    11701 taaacccctt gccaatccct taatgcgcaa tacagttggt cgtaaagtat cacttaaaca
    11761 gggttctaag ctagcaggaa ctcctatgga caagattgta cgcacactgg aagcgaatgg
    11821 ctacgaagtg attggattag actaatgaca gatgaacgga ttcatatcct acgggatatt
    11881 ttgttagaat tgcacaatgg cgcctctcct gagtcggttc aagatcgctt tgatgcgacc
    11941 tttacgggcg tgtcagccat cgagatttcc cttatggagc acgagctgat gaactcggat
    12001 tcgggcgtca cttttgaaga tgttatggaa ctctgtgatg tccatgccaa tctttttaaa
    12061 aatgctatca aaggtgtcga agtttcagat actgagcatc caggtcaccc agttcgtgtc
    12121 ttcaaagaag aaaatctggc tctccgtgcg gccttgattc gcattcgtag attgttagat
    12181 acctatgagt ctatggaaga cgaggaaatg ctggcggaga tgcgtaaggg tttggtgcgt
    12241 cagatgggac ttgtgggtca atttgacatc cattaccaac gtaaggaaga actcttcttt
    12301 cctatcatgg agcgctatgg acacgattca cctcccaaag ttatgtgggg agtggatgat
    12361 cagattaggg aactctttca aacagctcta acgacagcca agtcactacc agaagtgtca
    12421 attagcagtg taaaggaagc ttttgaagct tttgcgacag agtttgaaag tatgattttc
    12481 aaggaagagt ccatcctcct catgattctc cttgagtctt ttactcagga tgactggctt
    12541 cagattgcgg aggagagcga tgcctatggc tatgccatca tccgtccgtc agagaaatgg
    12601 gtgccagaac gacagagctt tattgaggaa aagattgcag aggagcctgt acagctagat
    12661 acggcagaag gtcaagttca acaagtcata gatacgccag aaggccattt taccattacc
    12721 tttaccccta aggaaaagga agctgtgctg gaccgccata gtcaacaggc ttttggtaat
    12781 ggctatcttt cagtcgagca ggccaatctc atcctcaatc atctccctat ggagattacc
    12841 tttgtcaata aagaagatat tttccagtat tacaatgaca atacgccagc tgatgagatg
    12901 attttcaaac ggacgccgtc ccaagtcggg cgcaatgtcg aactctgcca tccgcctaag
    12961 tacttggaca aggtcaaaac tatcatgaag gggcttcgtg agggaagcaa agacaagtat
    13021 gaaatgtggt tcaagtctga gtcgcgaggt aagtttgtcc acatcaccta tgctgcagta
    13081 cacgatgaag acggagaatt ccaaggagtg ttggagtatg ttcaggatat ccagccctac
    13141 cgtgagattg atacggacta ttttcgtgga ttagaataag gagaaaaaat gagttacgaa
    13201 caagaattta tgaaggaatt tgaagcttgg gtcaataccc aaatcatgat taacgatatg
    13261 gcgcacaagg aaagtcaaaa agtttacgaa gaagaccagg acgagcgtgc caaagatgcc
    13321 atgattcgct acgagagtcg cttggatgct tatcagttct tgcttggtaa gtttgaaaac
    13381 ttcaaagcag gcaagggatt ccatgatttg ccagaaggct tgtttggtga gcgaaattat
    13441 taaacgagaa agattcttga tttttcacta aaatcttgat agaatgttta tgttaaatcc
    13501 ttgtcagagc agggattttt tattgaaagg attttatcat gtcaaagaaa ctcaatcgta
    13561 aaaaacaatt acgaaatggc ctccgtcgcg caggtgcctt ttcaagtact gtgactaagg
    13621 ttgtagatga gacaaaaaaa gtcgtgaagc gtgcagaaca gtcagcaagc gcagctggta
    13681 aggctgtttc taaaaaagtt gaacaagcag tagaagctac caaagagcaa gctcaaaaag
    13741 tagctaattc tgtagaagat tttgcagcaa atttgggtgg acttccactt gatcgtgcca
    13801 agactttcta tgatgaagga atcaagtctg cttcagattt caaaaactgg actgaaaaag
    13861 aactccttgc cttgaaagga atcggcccag ctaccatcaa gaaattgaaa gaaaatggca
    13921 tcaagttcaa gtaatttttc ttgagccttg catttccgaa aaaatcttgc tacaatagag
    13981 ccattagagg tgttttgaat cccacatttt acagaaagtg gcggcgctga gaagtccaca
    14041 aatgtgtcaa aactggttgc taatggatga aaaattgaaa taaaagtgtc tttttgcttt
    14101 aaagacgaga gttgcgggca actgcccgaa attgggtggt accgcggata aacacattcg
    14161 tccctgtcat gtagatggca gggcttttct tttacgtctt agtcaaaggg ggttgttatg
    14221 aagcaatctt ttaacacaag taaactatat tatggttttc ctgtttttat tttaggatat
    14281 caagattaga attttggcta taacatcacg acctgtagtt cctcttatag tctaggagat
    14341 tgggttgtga ttggagtcgt tgcaagagag aatgccgcag agcagatcaa acagtatcaa
    14401 aaatttactg tgaatatttc tgatgaaact tctatgcttg cgatggagca ggctggtttt
    14461 atcagtcatc aggagaaatt ggaacgtttg ggagtgcatt atgaaatttc tgaatgaact
    14521 cagactccta ttttagacgc ctgtccactt gttttagatt gtcgggtaga taggattgtt
    14581 gaggaagacg gtatttgcca catctttgcc aagattcttg agcgacttgt tgttccagaa
    14641 ctcctggatg aaaagggaca ttttaaaaat caactgtttg ccccaaccta tttcatggga
    14701 gatggatatc agcgcgttta tcgctatctg gacaagcgtg tagatatgaa gggcagtttc
    14761 atcaaaaaag cgaggaagaa ggatggcaag aactgagctg ccagataaaa tcgaaacaga
    14821 acgtctcgtt ttacgagtcc gtactgtggc ggatgctgag gatatctttg actatgctag
    14881 tttgccagag gttgcctatc cagcaggttt tcctccagtc aagaccttgg aagatgagat
    14941 ttattatctg gagcacattc ttccggagcg taatcaaaag gaaaatctcc cagcaggcta
    15001 cgggattgtc gtcaaaggaa cggataagat cgttggctct gtcgatttca accatcgcca
    15061 tgaagatgat gtgctggaaa tcggctatac cttacaccca gactattggg ggcgaggtta
    15121 tgttccagaa gcagcgtgtg ccttgattga tctagctttt aaagaacttg gtcttcacaa
    15181 gattgaacta acttgctttg gatataacct tcaaagtcaa cgagtctcgg aaaagcttgg
    15241 ctttaccctc gaagctcgca taagagaccg aaaggatgtt caaggaaacc gctgtgacag
    15301 tctgatatat ggcttgctga agagcgagtg ggaggaataa gatgagcgat gtaaaagagg
    15361 aagtaagctc tctatctgag aagcagttac gccaaattga tgttgagtat gctgaattaa
    15421 atgatagtga tattatcgag cgattagctt atttagaaat taacaacaat gaaaaacgta
    15481 ttgtcatctc tgatattgaa ccaaccaagg aaataatgtc agtatcggac caaatttttg
    15541 agattcaaaa gaattttcaa aaaatcaaga atatgttcga attgtttatt tcagatgtct
    15601 cggatttttt aagtattaaa aataaacttg agtcaaagga attagaaatt gaagaggcag
    15661 atgtaaatcg tttcatgatt catttattat ccagtggaaa attgtttgtc gattttaacg
    15721 aaaaccaaat taaacaaaaa tattccaaag atagtgagga gtttgattgt atacatggtt
    15781 ttgcaagcta tcagtatgat accaattttg cttatcgatt ttgccattct ttgagaaatt
    15841 attcgcaaca tactgactta ccaataaatg aggtaaaagc tgttagccca gatgatgaga
    15901 ctgttataat agatttctat attgatttag attatctttt aaattccaac tttaaatgga
    15961 aaaaattgaa aggggagttg ataaaactaa accaagaaac atctaagatt gacgctatag
    16021 ctctagtgaa agaatac
//
LOCUS       DAVLXF010000039        15726 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLXF010000039 DAVLXF010000000
VERSION     DAVLXF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.039"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            211..1998
                     /locus_tag="VYO12_001211"
     CDS             211..1998
                     /locus_tag="VYO12_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1587828.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYIAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEERGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1995..2471
                     /locus_tag="VYO12_001212"
     CDS             1995..2471
                     /locus_tag="VYO12_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1587829.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2499..3404
                     /locus_tag="VYO12_001213"
     CDS             2499..3404
                     /locus_tag="VYO12_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1587830.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3391..4206
                     /locus_tag="VYO12_001214"
     CDS             3391..4206
                     /locus_tag="VYO12_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1587831.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4213..4617
                     /locus_tag="VYO12_001215"
     CDS             4213..4617
                     /locus_tag="VYO12_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1587832.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4916..6076
                     /locus_tag="VYO12_001216"
     CDS             4916..6076
                     /locus_tag="VYO12_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1587833.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATILVSFARSGNSPESVATVDLAKSLVDELYQVTITCATDGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEY"
     gene            6198..7235
                     /locus_tag="VYO12_001217"
     CDS             6198..7235
                     /locus_tag="VYO12_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1587834.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSITPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPVGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7442..8296
                     /locus_tag="VYO12_001218"
     CDS             7442..8296
                     /locus_tag="VYO12_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1587835.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIVQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            8446..8703
                     /locus_tag="VYO12_001219"
     CDS             8446..8703
                     /locus_tag="VYO12_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1587836.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8871..8999)
                     /locus_tag="VYO12_001220"
     CDS             complement(8871..8999)
                     /locus_tag="VYO12_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HEV1587837.1"
                     /translation="MDRTDEVSNHIYGKVKLMWFEEIFEEYKFLHNKTHITKVLMN"
     gene            complement(9033..9125)
                     /locus_tag="VYO12_001221"
                     /pseudo
     CDS             complement(9033..9125)
                     /locus_tag="VYO12_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9161..9457)
                     /locus_tag="VYO12_001222"
                     /pseudo
     CDS             complement(9161..9457)
                     /locus_tag="VYO12_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(9447..9608)
                     /locus_tag="VYO12_001223"
     CDS             complement(9447..9608)
                     /locus_tag="VYO12_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1587838.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            9678..10442
                     /locus_tag="VYO12_001224"
     CDS             9678..10442
                     /locus_tag="VYO12_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1587839.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            11055..12434
                     /locus_tag="VYO12_001225"
     CDS             11055..12434
                     /locus_tag="VYO12_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1587840.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIASRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12448..13113
                     /locus_tag="VYO12_001226"
     CDS             12448..13113
                     /locus_tag="VYO12_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1587841.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13227..13322
                     /locus_tag="VYO12_001227"
                     /pseudo
     CDS             13227..13322
                     /locus_tag="VYO12_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            13468..14007
                     /locus_tag="VYO12_001228"
     CDS             13468..14007
                     /locus_tag="VYO12_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1587842.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            14074..14484
                     /locus_tag="VYO12_001229"
     CDS             14074..14484
                     /locus_tag="VYO12_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1587843.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            14600..>15726
                     /gene="pavB"
                     /locus_tag="VYO12_001230"
     CDS             14600..>15726
                     /gene="pavB"
                     /locus_tag="VYO12_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1587844.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADG
                     LNPTPGQVLPEETPGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     SPSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLG"
BASE COUNT         4391 a         2998 c         3586 g         4751 t
ORIGIN      
        1 ttctatacaa aattatttta ggtcctatat tccgaattta aagagcgttt gaaatgggtc
       61 taaaaatgtt tgaaactaat tgaatgtgtg tataatagta aaaatgcagt aaatatcccg
      121 acaatttcta ttgacaattc aaacagattg gtttataatt aatataacaa caaatgaaag
      181 cgcaaacttt cgcggtcgga aggtagtttt atgacacgat ttgagatacg agatgatttc
      241 tatctcgatg gaaaatcatt taagatttta tctggtgcca ttcattattt tagggttcct
      301 ccagaggatt ggtatcattc gctctataac ttgaaggctc ttggttttaa tacggtagag
      361 acttatattg cttggaattt acacgagcct cgtgaaggtg agtttcattt tgacggtgat
      421 ctggatttag agaaatttct ccaaatagcg caggatttgg gtctctacgc aattgtgcgt
      481 ccgtctccat ttatctgtgc ggaatgggaa ttcggtggct taccagcttg gctcttgacc
      541 aagaacatgc gaattcgctc atccgaccca gcatatatcg aggcagttgg tcgctactat
      601 gatcagttat tgccaagact ggtgcctcgt ttgttggaca atggtggcaa tattctcatg
      661 atgcaggttg aaaatgagta tggttcttac ggagaagata aggcttacct gagagcgatt
      721 cgacagctaa tggaagagcg tggcgtaacc tgtcctctct ttacatcaga tggtccatgg
      781 cgagctactc tgaaagctgg aaccttaatt gaagaggacc tctttgtaac aggaaacttt
      841 ggttctaagg caccttacaa cttttcgcag atgcaggaat tctttgatga acatggtaag
      901 aaatggccac tcatgtgtat ggagttctgg gatggttggt ttaatcgctg gaaagaaccg
      961 attatcacac gggatcctaa ggaattggca gatgcagttc gagaggtttt ggaacaaggc
     1021 tctatcaatc tttacatgtt ccacggtggt acaaactttg gtttcatgaa tggttgctcg
     1081 gctcgaggaa ctttggacct gccacaagtt acgtcttatg attacgatgc ccttctggat
     1141 gaagaaggaa atccaactgc taaatatctt gcagtcaaga agatgatggc aacacatttt
     1201 tcagagtatc cgcagttgga accactctac aaagagagta tggagttgga tgctattcca
     1261 ctagttgaaa aagtttcttt gtttgaaacc ttagatagct tgtcaagtcc tgtagaaagt
     1321 ctctatcctc aaaagatgga ggagctggga caaagttatg gctacctact ttatcgaaca
     1381 gaaacaaact gggatgcaga agaagaaaga cttcgtatca ttgatggtcg agatagggcc
     1441 cagctgtatg tcgatggtca gtgggttaaa actcaatatc agacagagat tggggaagat
     1501 attttttatc aaggtaaaaa gaaagggcta tctaggttag atatcttgat agaaaatatg
     1561 gggcgtgtca actatgggca taagttctta gcggatacgc aacgtaaggg aattcggaca
     1621 ggggtctgta aggatctgca tttcttacta aactggaaac actatccact tccactagac
     1681 aatcctgaga aaattgattt ttcaaaagga tggactcaag gacaaccagc cttttacgct
     1741 tatgacttta cagtcgaaga gccaaaagat acttacctag acttgtctga gtttggtaag
     1801 ggagttgcct ttgtcaatgg gcagaatcta ggacgttttt ggaacgttgg cccaactctc
     1861 tcactttata tccctcatag ctatctcaag gaaggtgcca accgcatcat tatctttgaa
     1921 acagaaggtc aatataaaga agagattcat ttaactcgta aacctacact aaaacatata
     1981 aagggggaaa acttatgaca attgtaggat gccgtattga tggacgtttg atccacggac
     2041 aagtagccaa tctttgggct ggaaaactaa atgtttcacg cattatggtt gtagacgacg
     2101 aagttgtcaa caacgatatt gaaaagagtg gtttgaaact tgcgacacca ccaggtgtga
     2161 aattgagtat tttgccagtt gagaaagctg cagccaatat tcttgctggt aaatacgata
     2221 gccaacgtct ctttatcgtg gctcgtaaac cagaccgctt ccttggcttg gtagaagcag
     2281 gtgtaccact tgaaaccctt aatgttggga atatgtctca aacaccagaa actcgttcta
     2341 ttacacgttc tatcaacgta gtagacaagg atgtggaaga cttccacaaa ctggcagaaa
     2401 aaggtgttaa acttactgct cagatggttc caaatgatcc aatttcagac tttttgagct
     2461 tattaaaata ggaaaaaaat ttttaggagg tcattgttat gatacaatgg tggcaaattt
     2521 tacttctcac tttgtactca gcttatcaaa tctgtgatga gttgacgatc gtttcatctg
     2581 caggttcccc tgtatttgct ggtttcatta ctggtttaat catgggagat gtgactactg
     2641 gtttacttat cggtggtaac ttgcaactgt tcgttcttgg ggttggtacc ttcggtggtg
     2701 cttctcgtat cgacgcaact tctggtgcgg ttcttgcgac agccttctct gtttcacaag
     2761 gaattgatgc accgcttgcc attactacaa tcgctgtacc agtagcagct ctcttgactt
     2821 acttcgacgt tcttggtcgt atgactacta ccttcttcgc tcaccgtgtg gatgctgcaa
     2881 tcgaacgctt tgactataaa ggtattgaac gcaactactt gcttggtgcg attccgtggg
     2941 ctctatctcg tgcccttcca gtcttctttg cccttgcttt tggtggtgcc tttgtacaat
     3001 cagtagtaga cttcgttgaa gcctacaaat gggttgcaga tggcttgaca cttgcaggac
     3061 gtatgcttcc aggtcttgga tttgcaatct tgcttcgtta ccttccagtt aaacgtaacc
     3121 ttcactacct tgctatggga tttggtttga cagctatgtt gactgttctt tactcatatg
     3181 taacaggtct tggtggcgct gttgctggta tcgtaggtac tcttcctgct gaagttgctg
     3241 aaaaaattgg tttcgtgaac aacttcaaag gtttgtctat gattggtatt tctatcgtag
     3301 gtattttcct tgcagtgctt cacttcaaaa atagccaaaa agtagctgta gcagcacctt
     3361 ctacaccatc agaaagtggg gaaatcgaag atgacgaatt ctaattacaa acttacaaaa
     3421 gaagatttta atcaaatcaa caaacgtagc ttgtttactt tccaattagg ttggaactac
     3481 gaacgtatgc aagcttctgg ttacctttac atgatcttgc ctcagttgcg taaaatgtat
     3541 ggtgatggaa ctcctgaatt gaaagaaatg atgaaagttc atactcaatt cttcaatact
     3601 tcaccattct tccataccat tatcgctggt tttgaccttg ccatggaaga aaaagatggt
     3661 gtaggttcaa aagacgctgt taacggtatc aagacaggtt tgatgggacc attcgctcct
     3721 cttggggata caatctttgg ttcacttgta cctgctatca tggggtcagt cgcagcaact
     3781 atggctatcg ctggccaacc ttgggggatc ttcctttgga ttgcagttgc agtagcgtat
     3841 gacatcttcc gttggaaaca gttggaattt gcttacaaag aaggggttaa ccttatcaac
     3901 aacatgcaaa gtaccttgac agctttgatt gacgctgcat ctgtacttgg tgtcttcatg
     3961 atgggtgctc ttgtagcaac agtgattaac tttgaaattt cttacaagtt gccaatcggt
     4021 gaaaagatga ttgatttcca agacatcttg aaccaaatct tcccacgttt gcttccagca
     4081 atctttactg cctttatctt ctggttgctt ggtaagaaag gtatgaactc tactaaagct
     4141 atcggtatta ttatcgtact tgctttggct ctttctgccc ttggtcactt tgcacttgga
     4201 atgtaattcc ttatgactaa atcattaatt ttggtgagcc atggtcgctt ctgtgaggag
     4261 cttagaggta gcacagaaat gattatgggc ccacaagaca acatttacac agtagctctt
     4321 cttccagaag atggcccaga agaatttact gctaaatttg aagctgttat tgaaggattg
     4381 gatgatttcc tagtttttgc ggatcttctc ggtgggacac cttgtaatgt ggtgagtcgc
     4441 ttgatcatgg aaggtcgtga tattgacctt tacgcaggga tgaatcttcc aatggtgatt
     4501 gaatttatca atgcgagcct tacaggcgca gatgcggact acaagagccg tgctgcagaa
     4561 agcattgtga aagttaatga cctgttagcg ggcttcgatg atgacgaaga tgaataatac
     4621 tcttcgaaaa tctcttcaaa ctacgtcaac gtcgccttac cgtaggtata tgttactgac
     4681 ttcgtcagtc ttatccggca acctcaaaac ggtgttttga gctgacttcg tcagtcttat
     4741 ccggcaacct caaagcagtg ctttgagcag cctgcggcta gtttcctaca gattttagtt
     4801 ggaactcgat tcaattcatg tgacaacgtg aaaatcgtta gagcatttta tatagaatat
     4861 acatgggaat ggagcttact cccattccta tatttaatag aaaaagagga actcaatgct
     4921 acattataca aaagaagact tgctcgaatt gggtgcagaa atcactacgc gtgaaatcta
     4981 ccaacagcct gatgtatgga gagaagcttt tgaattttat caagcaaaac gtgaagaaat
     5041 tgcagccttc ctacaagaaa tcgctgataa acatgactat attaaggtta tcttgacagg
     5101 tgctgggact tctgcttatg tgggagacac cttgctacct tactttaagg aagtctatga
     5161 cgaacgcaaa tggaatttca atgctattgc gacaacagat atcgttgcca atccagcaac
     5221 ctatttgaaa aaagatgtgg caactatcct tgtgtctttt gctcgtagtg ggaactcgcc
     5281 tgaaagtgtg gcgactgttg atttggccaa atccttggtg gatgagcttt atcaagtgac
     5341 gattacttgt gcaacagatg gtaaattggc tcttcaagct cacggtgatg accgtaatct
     5401 cttgctcttg caaccagctg tctctaatga tgctggattt gccatgactt ctagctttac
     5461 gtctatgatg ttgacagctc tcttggtctt tgatcctaca gaatttgctg ttaagtctga
     5521 acgttttgaa gttgtatcta gtcttgcccg taaagtttta gacaaggcag aagatgtcaa
     5581 agagctcgtt gatttagact ttaaccgtgt catctatcta ggcgctggtc ctttctttgg
     5641 acttgctcat gaagctcagc tcaagatttt ggaattaact gctggtcaag ttgcgaccat
     5701 gtatgaaagc ccagttggct tccgtcacgg tccaaaatct cttatcaacg acaatacagt
     5761 tgttttggtc tttggtacaa cgacagacta cactcgtaag tacgacttgg acttggttcg
     5821 tgaagttgct ggtgaccaga ttgctcgtcg tgttgtgctt ttgagtgatc aagcttttgg
     5881 tcttgaaaat gtcaaagaag tggcccttgg ttgtggcggt gtcttgaatg atatttaccg
     5941 tgtcttccct tacatcgttt atgcccaact ctttgcccta ttgacttcac tcaaggtaga
     6001 aaataaacca gatacaccgt ctcctacagg tacagtaaac cgtgtagtac aaggtgtcat
     6061 aattcacgaa tattaaaagt aagacagtgt ttatgaattc ttgacaagag gatttgtaaa
     6121 ttatcagata aaccatagat tgtcagtacg ctttctatgg tttgtttgct tgagagaaat
     6181 agtaaaagga gaacagaatg aaagcataca cagagcgtgt atttggaaat gttgagggcg
     6241 aggatgtctt ggcctatcga tttgagacag acggtggcta ccaacttgag gttatgactt
     6301 atggcgcgac catcttgcgc tatgtcgcac ctgacaaggc tggaaatttt gccaatgtta
     6361 tcttgggatt tgatgacttt gatagttatg taggcaatag tcccaagcat ggagcaagtg
     6421 taggtcctgt agcgggtcgt attgcaggtg cgacctttga gctcaatggt aagacctatg
     6481 accttgaggt taataatgct agcaactgta atcacagtgg ttcaactggt tgggattcca
     6541 gcttgtttga agttgaagaa gtaagcgatc atggcttgac tctctacaca gaacgtacag
     6601 atgggacagg agggttccct ggaaatctca agatttggat cagttatcac ttggaagaaa
     6661 ctggtgccta tgaaattagc tacaaggtaa cgaccgatca ggatacgctg gtcaatccaa
     6721 ccaaccacag ctatttcaac ttgtctggtg atttcacgca gacgattgac cgtcatgtct
     6781 tccaactaaa cacagagggc atttactcaa tcactcctga cggtgttcct gccaaaactc
     6841 cagaagccaa ccgtgatgtg gtcaaacaca tctacaatgg taccttgttg aaggatatct
     6901 ttgcagaaga agatgagcaa atccagctgg catcaggttt ggatcaccca tttgcccttc
     6961 ctgtaggcca tgacaatgct ggattccttt atgaccaaaa ttcaggtcgc ttcctgcttt
     7021 tcaagacaga agctccttgc tttgtggtct acacagcaaa ctttgtggat gaaagtgtca
     7081 tcataggagg tcagccaatg ctacagcaca atgggattgc tcttgaagcg caagctttac
     7141 cagatgccat tcacagtgac cttaaaggcc aagtcattct taaagctggt caaaccttca
     7201 ccagtaagac acgttatgaa cttgttgtga aataaaaaag ctggtcaaaa agtctttaaa
     7261 attaaaataa cgcatattgt caggtgatga aaagtttgat actatgcgtt ttattatgaa
     7321 atgatttact ttatcttatc ctgaaatggt gttatttttg agttatctgg cttgactttc
     7381 ttgatggaaa ttatataata gttgtaataa caataattac aacgagaaaa gaggaccatt
     7441 tatgacctat gaatacaaga gtcacattta tttggcagag acagttttaa atgtaaagga
     7501 tttggccagt caaacagcct tttatcagca agttattggt ttagaaatct tatctcaaac
     7561 tgagacagag tccattctag gccttggtgg aaaagtcttg gtacagctga ttcaagcaca
     7621 agagagcgga gaagtgaggg aacattatgg tctttaccat ttggccattc tcttgcccac
     7681 acgcaaggct ttggctgatg tcttgaaaca cctgacagat ttacagattc ctcttgtcgg
     7741 cggtgcagac catggttaca gtgaagctat ttacttggaa gatttggagg gaaatggcat
     7801 tgaactctat cgagataagc cagtttccac atgggatatt cgagaagatg gacgcattat
     7861 cggagtgacg gaggctcttg cggcgcagga catttatgag ttgggggaaa gagtagagcc
     7921 ctttatcctg gcagaaggta caagaatggg gcatattcat ctttccgtca aggatagtcg
     7981 aaagtccagc cagttttatc aaaaggtgtt agggctagag gataaattca gtgtgcctag
     8041 cgctagttgg attgcggctg gagattacca tcatcattta gcagttaacg aatggggagg
     8101 aaaaggtctg gcttcgcgta agcaaggctt accaggttta gcctactatg tcatcgaagt
     8161 cgcacataaa gaagaactgt taacgattgt ccaacgagca caagaagttg acgcaccaat
     8221 caaatggatg acatcgagcc aattggaaat cacagactca gatggcatcg tgacccgtat
     8281 tcgtttagca aggtaaaatt aggaatttct aataagttat aagtattgct agtttgcttt
     8341 ttgaacggtc tttggtaaca gaactgcgtg agctggatgg gtgagaattt tgacaaagaa
     8401 gctgattaga gatataatga agaaaaatcg agcaaggaaa aagaaatgac aaattcaaag
     8461 tatattactt gtttgaaacg ttcagagggc cagttgtgtg ggattcaaaa gatgattgaa
     8521 ggagatcgtg actgtgctga tattgtgacg caactgacag cagtgagatc tagtgtagag
     8581 cgcgtgattg agatgataat taccgaaaat cttactgaat gtatcaatca gccactagat
     8641 gattctgaag ctcaaaagga acgcctagaa aaggctatcc gatacttgat taaacggaaa
     8701 taaatgaatg ttttgagacg atatgtgcaa aggtctatta actattagaa tagtttaatg
     8761 tgagtcaaaa gaagttgtaa aaaatcttta aaattaaaaa aacgcatagt atcaggtgtt
     8821 gaatgtactg acccccaaaa gttagatttt ttctgtctaa cttttggggg tcagttcata
     8881 agaaccttgg taatatgcgt tttattatgt agaaacttat actcttcgaa aatctcttca
     8941 aaccacatca gcttcacctt gccgtagata tggttactga cttcgtcagt tctatccaca
     9001 acctcaaaac agtgttttga gctgacttcg tcagttccat ccacaacctt aaaacagtgt
     9061 tttgagcagc ctgcggctaa cttcctagtt tgcactttaa ttttcattga gtattacttc
     9121 atcttctcat gaaattgagt ttttgcccag ctcctcttga ttattctact tgaccgggcc
     9181 aagtattcat accaccttct acgttggtaa cggtgaggcc ttgggagctg agaaattggc
     9241 aggtagatgc agagcggact cctccttgac aaataacata gtattcatgg tcaggtttga
     9301 gttctttgta gccttgctct aaggtactta atggaagatt tttggcacct ggtgcatgtc
     9361 ctgcttggaa ttcatgtgct tcacggacat cgataagttc tagattttca ttttagtatt
     9421 ttccataaaa gtcagccatg ctgatactag tttccatatt ctactccttc tgggttagcc
     9481 attttgtata gtgaataagc gccgtcaagg ttttggacgg taaatcctgc ttgcttgagg
     9541 ataggctctg cgatatagct gcgcaaacca ctgtggcagc tgacgatgta ggcttgtttt
     9601 ttgtccagtt catcagcttc ctccttgaca ctttgtcagt ttttgataca atagtacaaa
     9661 attagaggag gtgggctatg attcagaaac atgcgattcc cattttagag tttgatgata
     9721 atcctcaggc ggttatcatg cccaatcacg aggggttgga tttgcagttg ccaaagaagt
     9781 gtgtctatgc ttttttaggt gaggagattg accgctatgc gagggaagta ggggcgaact
     9841 gtgttggcga attcgtttct gctaccaaga cctatccagt ctatgtcatc aactacaagg
     9901 gtgaggaggt ctgtctggct caggctcctg ttggctccgc tccagcagcc cagtttatgg
     9961 attggttgat tggctatggt gtggagcaga ttatctctac tgggacctgt ggtgtcctag
    10021 ctgatataga ggaaaatgcc tttctagtcc ctgttcgcgc tttgcgagat gagggagcca
    10081 gttaccacta tgtggcacct tgtcgttata tggaaatgca gccagaggct attgctgcta
    10141 ttgaggaagt tttggaagac agagggattc cttatgaaga agtcatgacc tggacgacag
    10201 acggttttta ccgagaaacg gctgaaaagg tggcttatcg taaggaagaa ggctgtgctg
    10261 ttgtggagat ggagtgttct gctcttgcgg cagtagctca attgcgtggg gttctctggg
    10321 gtgaattgtt gttcacagca gattctctag cggacttgga ccagtacgac agtcgtgact
    10381 ggggctcgga agcttttaat aaggcgctag aactgagttt agcaagtgtt caccaccttt
    10441 agttgtacag gcaaaggatt tgttttatca taaaatgtct agctcatact tttcaaaaat
    10501 atgtttaaac gaggtcacct tcctcttgtc ctaggcatgt tgaggttggg aaaaatcttt
    10561 aaaatcagaa aaacgtatca tatcaggtga tgaaaatttt gatactatgc gttttatgtt
    10621 ggtaagattt agagtgagat gaaatgatac tcttcgaaaa tctcttcaaa ccaggtcagc
    10681 ttcaccttgc cgtaggtata tgttactgac ttcgtcagtc ttatccggca acctcaaaac
    10741 ggtgttttga gctgacttcg tcagttctat ccacaacctc aaaacagtgt tttgagcaac
    10801 ctgtgactag ctttctaagc gatgccttgg ttctcattgc ctataatcaa aaagagaaat
    10861 tttctcctga aaagcatata gagtagctgg cgttaaaagc tcctgtcttg cttttttgac
    10921 ctatagtcac atctatcaag tattgttctt gcctaagcta tcaataaaaa ggtggcattt
    10981 tttaggcttg gtgttagtga attttgcctt atcctatcta agtcatttcg agctttttat
    11041 ggtacaatgg aaacatgtta ttcaaattat ctaaggaaaa aatagagcta ggcttatctc
    11101 gtttatcgcc agcccgtcgt atttttttga gttttgcctt ggtcatttta ctaggctctc
    11161 ttcttttgag cttgcccttt gtccaagttg aaagctcacg agcgacttat tttgatcatc
    11221 ttttcactgc tgtctctgca gtctgtgtga caggtctctc aacccttcca gtagctcaca
    11281 cctataatat ctggggccaa ataatctgtt tgctcttgat tcagatcggt ggtctagggc
    11341 tcatgacttt tattggggtt ttctatatcc agagcaagca aaagcttagt cttcgtagcc
    11401 gtgcaactat tcaggatagt tttagttatg gagaaactcg atctttgaga aagtttgtct
    11461 attctatttt tctcacgacc tttttggttg agagcttggg agctattttg cttagttttc
    11521 gccttattcc tcaacttggc tggggacgtg gtctttttag ttccattttt ctagcgatct
    11581 cagccttctg taatgccggt tttgataatt tagggagcac cagtttattt gcttttcaga
    11641 ccgatttact ggtcaatctg gtgattgcag gcttgattat tacaggcggc cttggtttta
    11701 tggtctggtt tgatttggct ggtcatgtag gaagaaagaa aaaaggacgt ctgcactttc
    11761 atacgaagct tgtactattg ttgactatag gtttgttgtt atttggaacg gcaactactc
    11821 tctttcttga gtggaacaat gctggaacga ttggcaatct ccctgttgcc gataaggttt
    11881 tagttagctt ttttcaaaca gtgacgatgc gaacagctgg cttttctacg atagattata
    11941 ctcaggctca tcctgtgact cttttgattt atatcttaca gatgtttcta ggtggggcac
    12001 ctggaggaac agctggggga ctcaagatta cgacattttt tgtcctcttg gtctttgcac
    12061 gaagtgagct tctaggcttg cctcatgcca atgttgcgag acgaacgatt gcgtcgcgaa
    12121 cggttcaaaa atcctttagt gtctttatta tctttttgat gagcttcttg ataggattga
    12181 ttctgctagg gataacagcc aaaggcaatc ctccctttat ccacctcata tttgaaacca
    12241 tttcagctct tagtacagtt ggtgtaacgg caaatctgac tcctgacctt gggaaattgg
    12301 ctctcagtgt tatcatgcca cttatgttta tgggacgaat tggtcccttg accttgtttg
    12361 ttagcttggc agattaccat ccagaaaaga aagatatgat tcactatatg aaagcagata
    12421 ttagtattgg ttaagaaagg aaagagcatg tcagatcgta cgattggaat tttgggtttg
    12481 ggaatttttg ggagcagtgt cctagctgcc ctagccaagc aggatatgaa tattatcgct
    12541 attgatgacc acgcagagcg catcaatcag tttgagccag ttttggcgcg tggagtgatt
    12601 ggtgacatca cagatgaaga attattgaga tcagcaggga ttgatacctg cgataccgtt
    12661 gtagtcgcga caggtgaaaa tctggagtcg agtgtgcttg cggttatgca ctgtaagagt
    12721 ttgggggtac cgactgttat tgctaaggtc aaaagtcaga ccgctaagaa agtgctagaa
    12781 aagattggag ctgactcggt tatctcgcca gagtatgaaa tggggcagtc tctagcacag
    12841 accattcttt tccataatag tgttgatgtc tttcagttgg ataaaaatgt gtctatcgtg
    12901 gagatgaaaa ttcctcagtc ttgggcaggt caaagtctga gtaaattaga cctccgtggc
    12961 aaatacaatc tgaatatttt gggtttccga gagcaggaaa attccccatt ggatgttgaa
    13021 tttggaccag atgacctctt gaaagcagat acctatattt tggcagtcat caacaaccag
    13081 tatttggata ccctagtagc attgaattcg taaagaggga tgacccctct tttttgatgc
    13141 ccaagatggc aaatagagac agaagcccct tgtcttctag taaaagttct tcaaaggctg
    13201 gactttatgg taaaatagaa ggaagtgaca agagagagta atactcaatg aaaatcaaag
    13261 atcaaactag gaaactagct gcgggctgct caaaacactg ttttgaggtt gtggatagaa
    13321 ctgacgaagt cagctcaaaa cactgttttg aggttgcaga tagaactgac gaagtcagta
    13381 acatctatac ggcaaggcga cgttgacgcg gtttgaagag attttcgaag agtataagaa
    13441 aaaatcagtc ccctaaagga gcagattatg aagttattgt ctatcgccat ttctagctat
    13501 aatgcagcag cctatcttca ttactgtgtg gagtcgctag tgattggtgg tgagcaagtt
    13561 gggattttga ttatcaatga cgggtctcag gatcagactc aggaaatcgc tgagtgttta
    13621 gctagcaagt atcctaatat cgttagagcc atctatcagg aaaataaatg ccatggcggt
    13681 gcggtcaatc gtggcttggc agaggcttct gggcgctatt ttaaagtagt tgacagtgat
    13741 gactgggtgg atcctcgtgc ctacttgaaa attcttgaaa ccttgcagga acttgagagc
    13801 aaaggtcaag aggtggatgt ctttgtgacc aattttgtct atgaaaagga agggcagtct
    13861 cgtaagaaga gtatgagtta cgattcagtc ttgcctgttc ggcagatttt tggctgggac
    13921 caggtcggaa atttctccaa aggccagtat accatgatgc actcgctgat ttatcggaca
    13981 gatttgttgc gtgctagcca gttctaactg cctgaacata ctttttatgt cgataatctc
    14041 tttgtcttta cgccccttca gcaggtcaag accatgtact atctgcctgt cgatttctat
    14101 cgttatttga ttgggcgtga ggaccagtct gtcaatgagc aagtgatgat taagtgcatt
    14161 gaccagcaac tcaaggtcaa tcgactcttg atagaccaac ttgatttgtc ccaagtgagt
    14221 catcccaaaa tgcgagaata tctgctgaat catattgaac tcacgacggt gatttccagt
    14281 accctgctca accgatctgg aacagcggag catctggcaa aaaaacgcca attgtggacc
    14341 tatattcagc agaaaaatcc agaagtcttt caggctattc gtaagaccat gttgagccgt
    14401 ttgaccaaac attctgtctt gccagatcgc aaactgtcca atgtcgtcta tcaaatcacc
    14461 aaatctgttt atggatttaa ttaatataag tgttttataa gagggattta agaaaaattt
    14521 taactttttc ttagtccttt ttaatttcag gagattatac tagagtcatc aaataaagaa
    14581 agactctaag gagaatccta tgaaattcaa tccaaatcaa agatatactc gttggtctat
    14641 tcgccgtctc agtgtcggtg ttgcctcagt tgttgtggct agtggcttct ttgtcctagt
    14701 tggtcagcca agttctgtac gtgccgatgg gctcaatcca accccaggtc aagtcttacc
    14761 tgaagagaca ccgggaacga aagagggtga cttatcagaa aaaccaggag acaccgttct
    14821 cactcaagcg aaacctgagg gcgttactgg aaatacgaat tcacttccga cacctacaga
    14881 aagaactgaa gtgagcgagg aaacaagccc ttctagtctg gatacacttt ttgaaaaaga
    14941 tgaagaagct caaaaaaatc cagagctaac agatgtctta aaagaaactg tagatacagc
    15001 tgatgtggat gggacacaag caagtccagc agaaactact cctgaacaag taaaaggtgg
    15061 agtgaaagaa aatacaaaag acagcatcga tgttcctgct gcttatcttg aaaaagctga
    15121 agggaaaggt cctttcactg ccggtgtaaa ccaagtaatt ccttatgaac tattcgctgg
    15181 tgatggtatg ttaactcgtc tattactaaa agcttcggat aatgctcctt ggtctgacaa
    15241 tggtactgct aaaaatcctg ctttacctcc tcttgaagga ttaacaaaag ggaaatactt
    15301 ctatgaagta gacttaaatg gcaatactgt tggtaaacaa ggtcaagctt taattgatca
    15361 acttcgcgct aatggtactc aaacttataa agctactgtt aaagtttacg gaaataaaga
    15421 cggtaaagct gacttgacta atctagttgc tactaaaaat gtagacatca acatcaatgg
    15481 attagttgct aaagaaacag ttcaaaaagc cgttgcagac aacgttaaag acagtatcga
    15541 tgttccagca gcctacctag aaaaagccaa gggtgaaggt ccattcacag caggtgtcaa
    15601 ccatgtgatt ccatacgaac tcttcgcagg tgatggcatg ttgactcgtc tcttgctcaa
    15661 ggcatctgac aaggcaccat ggtcagataa cggcgacgct aaaaacccag ccctatctcc
    15721 actagg
//
LOCUS       DAVLXF010000040        15668 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLXF010000040 DAVLXF010000000
VERSION     DAVLXF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.040"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            112..3213
                     /locus_tag="VYO12_001231"
     CDS             112..3213
                     /locus_tag="VYO12_001231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1587845.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQVDD
                     SKKKLDVTLFSDLYRQVDQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSDQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            3296..4303
                     /gene="pfkA"
                     /locus_tag="VYO12_001232"
     CDS             3296..4303
                     /gene="pfkA"
                     /locus_tag="VYO12_001232"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1587846.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4362..5867
                     /gene="pyk"
                     /locus_tag="VYO12_001233"
     CDS             4362..5867
                     /gene="pyk"
                     /locus_tag="VYO12_001233"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1587847.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6092..6385)
                     /locus_tag="VYO12_001234"
                     /pseudo
     CDS             complement(6092..6385)
                     /locus_tag="VYO12_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(6551..6776)
                     /locus_tag="VYO12_001235"
                     /pseudo
     CDS             complement(6551..6776)
                     /locus_tag="VYO12_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7624..8496
                     /locus_tag="VYO12_001236"
     CDS             7624..8496
                     /locus_tag="VYO12_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1587848.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8642..8986)
                     /locus_tag="VYO12_001237"
                     /pseudo
     CDS             complement(8642..8986)
                     /locus_tag="VYO12_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9129..9440
                     /locus_tag="VYO12_001238"
     CDS             9129..9440
                     /locus_tag="VYO12_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587849.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            9444..10160
                     /locus_tag="VYO12_001239"
                     /pseudo
     CDS             9444..10160
                     /locus_tag="VYO12_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(10290..11105)
                     /locus_tag="VYO12_001240"
     CDS             complement(10290..11105)
                     /locus_tag="VYO12_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1587850.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11102..12007)
                     /locus_tag="VYO12_001241"
     CDS             complement(11102..12007)
                     /locus_tag="VYO12_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1587851.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            complement(12382..12465)
                     /locus_tag="VYO12_001242"
                     /pseudo
     CDS             complement(12382..12465)
                     /locus_tag="VYO12_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            12509..14638
                     /locus_tag="VYO12_001243"
     CDS             12509..14638
                     /locus_tag="VYO12_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1587852.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIADLEKEAEKFVCEGFATGKEALAGAVDI
                     LVEALSEDVTLRAMTYQEVLRHSKLTSQVKDESLDEKQVFQIYYNFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNINTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKQIDTEREKVNLSLLAPNETD"
     gene            14625..15074
                     /locus_tag="VYO12_001244"
     CDS             14625..15074
                     /locus_tag="VYO12_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1587853.1"
                     /translation="MKLTDYVKQVSLEDFGRSFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTRRYRCGLCRGKLLLINQPED"
     gene            15133..15405
                     /locus_tag="VYO12_001245"
     CDS             15133..15405
                     /locus_tag="VYO12_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1587854.1"
                     /translation="MNSKFYKMRRNRMISGVLVGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT         4832 a         2793 c         3376 g         4667 t
ORIGIN      
        1 atacaaatga atagaaaata aattagtaag ctgatgaaat ttttctcaag agaagccatt
       61 tataggtgaa aatggtataa tatagtgaga aggatagagg agaagtgtaa attgatcgca
      121 caactagata caaaaacagt ctatagtttt atggaaagcg tcatttcgat cgaaaagtat
      181 gtgagagcag ctaaagaata cggctacact catttggcta tgatggatat tgacaatctt
      241 tatggcgctt tcgactttct agagattaca aaaaaatacg gcattcatcc tttgctaggg
      301 cttgaaatga cagtgtttgt agatgatcag gaagtgaatt tgcgtttttt agctctatct
      361 agtgtgggct atcagcagtt gatgaagctt tcgacagcca agatgcaggg ggagaaaact
      421 tggtcagtcc tgtcccagta cctggaggat atcgcggtca ttgtgcctta ttttgataga
      481 gttgagtcgt tagaactagg ctgtgattac tatatagggg tttatccaga aacactagca
      541 agcgaatttc atcatcctat cttacctctt tatcgggtca acgcttttga aagcagggat
      601 agagaagttc ttcaagtttt aacagcgatt aaagaaaatc taccgctcag agaagttccc
      661 ttgcgttcga gacaagatgt ctttatatca gcaagttctt tagagaaact attccaagag
      721 cgttttccgc aagctttgga caatttagaa aagcttattt caggcatttc ttacgacttg
      781 gatactagtc tgaaactgcc tcgttttaat ccagctagac cagcagtaga ggagttgaga
      841 gagcgtgctg aactggggct tgttcagaag gggttgacta gtaaagaata tcaagataga
      901 ctagaccaag aattgtctgt tattcatgat atgggctttg atgattattt cttggttgtt
      961 tgggatttgt tgcgttttgg acgatcgaat ggctattata tgggaatggg aaggggttct
     1021 gcagtaggca gtttggtttc ttatgcctta gacatcacgg ggattgaccc agtagagaaa
     1081 aatctgattt ttgaacgctt tcttaatcgt gaacgctata ccatgcctga tattgatatt
     1141 gatattccag atatttatcg tccagatttt atcagatatg ttggtaataa atatggtagt
     1201 aaacatgcgg cacaaatcgt tactttttca acctttggag ccaagcaagc tcttcgagat
     1261 gtcttgaaac gctttggtgt gccagagtat gaattatctg caattactaa gaaaatcagt
     1321 tttcgtgaca atcttaagtc ggcctatgag ggcaatctcc agtttcgtca gcaaatcaat
     1381 agtaagttag aataccaaaa agcttttgag attgcttgca agatagaggg ctatccaagg
     1441 caaacctctg tccatgcggc tggtgttgta attagtgacc aagatttaac caactatatt
     1501 cctctaaagt atggtgatga aattccactg actcagtatg atgctcatgg agttgaggct
     1561 agcggacttt tgaagatgga ctttctggga ctacgaaatt tgacctttgt ccagaagatg
     1621 caagagttgc ttgctgaaat agaaggtatt caccttaaaa ttgaagaaat agatttggaa
     1681 gacaaagaaa cgttagattt atttgcctct ggaaatacaa aaggtatctt tcaatttgag
     1741 caaccaggtg ccattcgtct gcttaagcgt gtgcaaccag tctgttttga agatgtcgtc
     1801 gcgactactt ctctaaatcg accgggtgct agtgactata tcaataattt tgtggcaaga
     1861 aagcatgggc aggaagaagt gactgttctg gatccagtac tggaggatat tttggctcca
     1921 acctacggca taatgctcta tcaggagcag gttatgcagg ttgcccagcg acttgccgga
     1981 tttagtcttg ggaaagccga tattttgcgt cgggctatgg ggaaaaagga tgcctctgcc
     2041 atgcatgaga tgagggcttc ctttattcaa ggttcattag aagctggtca tactgtggaa
     2101 aaagcagagc aggtctttga tgttatggag aagtttgcag gttatggttt taacaggtca
     2161 cacgcctatg cctactcagc cttggccttc cagttggctt atttcaaaac gcattatcca
     2221 gccatttttt atcaggtcat gttaaattct tccaacagtg attacttaat agatgcactt
     2281 gaagcaggtt ttgaagtagc ctctctatcc atcaacacca ttccctatca cgataaaatt
     2341 gccaacaagg ccatctatct aggtttgaaa tccattaaag gagtcagtaa tgatttagct
     2401 ctctggatta ttgaaaatag accttattct aacattgaag attttatagc taaattacct
     2461 gagaattatc tgaaacttcc tctgctagaa cctttggtaa aagttggtct tttcgattca
     2521 tttgaaaaaa atcgtcaaaa agtatttaat aacttagcta atctatttga atttgtgaaa
     2581 gagttgggaa gtttgtttgg agatgctatt tatagttggc aggaatcgga agattggacg
     2641 gaacaagaaa aattttatat ggaacaagag cttttaggga taggtgtcag caaacatcca
     2701 ctacaagcta ttgcaagtaa ggctatttac ccgattaccc caattggaaa tttgtcagaa
     2761 aatagctatg ctattatctt ggttgaagtt cagaagataa aagtaattcg taccaaaaag
     2821 ggtgaaaata tggctttctt acaggtggat gacagtaaga aaaaattgga tgttacactc
     2881 ttctcagatt tatatcgaca ggttgatcag gaagtaagag aaggagcatt ctactatatc
     2941 aaagggaaag tccagtcacg tgacggtcgt ctgcaaatga ttgcacaaga aataagagaa
     3001 gcagtggctg agcgcttttg gattcaggtt aaaaatcatg actcggatca agagatgtca
     3061 cgtattttag accagtataa aggcccaatc ccagtcatca tccggtatga agaggaacag
     3121 aaaaccatcg tttctcccca tcattttgta gctaaatctg atgaattaga ggcgaaattg
     3181 aatggaatag ttatgaaaac gatttatcgc taaaaatacg gaaaatagaa gaattttaaa
     3241 atcaaatgtg atataatcaa gaagaatgtt aaaagaaaaa ggagcataaa ccaatatgaa
     3301 acgtattgct gttttgacta gtggtggaga cgcacctggt atgaacgctg ctatccgtgc
     3361 agttgttcgt caagcaattt cagaaggaat ggaagtgttt ggtatctatg acggatatgc
     3421 tggtatggtt gccggtgaaa ttcaccccct agatgcagct tcagtaggag acatcatttc
     3481 tcgtggtggt acttttcttc actcagctcg ttatccagag ttcgctcaac ttgaagggca
     3541 acttaaaggg attgagcaat tgaaaaaaca cggaattgaa ggtgtagttg ttatcggtgg
     3601 tgacggatct taccacggcg ctatgcgttt gactgaacat ggcttcccag ctattggtct
     3661 tccaggtaca atcgataacg atatcgttgg tactgacttt acaatcggtt ttgacacagc
     3721 ggttactact gccatggacg ctatcgataa gattcgtgat acatcatcaa gtcaccgtcg
     3781 tacttttgta atcgaagtta tgggacgtaa cgctggtgat atcgctcttt gggctggtat
     3841 tgcaactggt gctgatgaaa tcatcatccc tgaagcaggc ttcaagatgg aagatatcgt
     3901 agcaagcatc aaagctggtt atgaatgtgg taaaaaacac aatatcatcg tcttagctga
     3961 aggtgtgatg tcagcggctg aatttggtca aaaacttaaa gaagctggag atacaagcga
     4021 ccttcgtgta acagaacttg gacatattca acgtggtggt tctccaactg cgcgtgaccg
     4081 tgttttggcg tcacgtatgg gtgcacatgc tgttaaactt cttaaagaag gtatcggtgg
     4141 tgttgcggtt ggtattcgta acgaaaaaat ggttgaaaat ccaattcttg gtactgcaga
     4201 agaaggggca ttgtttagcc ttactgcaga aggtaagatt gtggttaaca acccacacaa
     4261 agccgatatt gagctatcta gcttgaataa gagcttgtca taatttacaa tttagttaat
     4321 aagaccaaaa aggtcatata tataaaggag tcacaaaaat catgaacaaa cgtgtaaaga
     4381 tcgttgcaac tttgggtcct gcggtagaaa tccgtggtgg taaaaaattc ggtgaggatg
     4441 gatactgggg tgaaaaactt gatgttgaag cttcagctaa aaacattgct aaattgattg
     4501 aagctggtgc taacacattc cgattcaact tctcacacgg cgaccaccaa gaacaaggtg
     4561 agcgtatggc aactgttaaa cttgcggaaa aaattgcagg taaaaaagtt ggtttccttc
     4621 ttgatacaaa aggacctgaa atccgtacag aattgttcga aggtgaagct aaagaatatt
     4681 catacaaaac tggtgaaaaa attcgtgttg caactaaaca aggaatcaaa tcaactcgtg
     4741 aagtgattgc gttgaacgtt gctggtgctc ttgatatcta tgatgatgtt gaagttggtc
     4801 gtcaagtttt ggttgacgat ggtaaacttg gtcttcgtgt ggttgctaaa gatgatgcaa
     4861 ctcgtgaatt tgaagttgaa gttgaaaacg atggtatcat cgctaaacaa aaaggtgtga
     4921 acatccctaa cactaaaatt cctttcccag ctcttgctga acgcgataac gacgatatcc
     4981 gtttcggtct tgaacaaggt atcaacttca tcgcaatttc attcgtacgt actgcaaaag
     5041 atgtgaacga agttcgtgca atctgtgaag aaactggaaa cggacatgtt caattgttcg
     5101 ctaaaatcga aaaccaacaa ggtatcgata acttagacga aatcatcgaa gcagctgatg
     5161 gtattatgat tgctcgtggt gatatgggta tcgaagtacc gttcgaaatg gttccagttt
     5221 atcaaaaaat gattatcaag aaagtcaatg ctgcaggtaa agttgttatc actgcaacaa
     5281 acatgcttga aacaatgact gaaaaaccac gtgcaactcg ttcagaagta tcagacgtat
     5341 tcaacgctgt tatcgacgga actgacgcta caatgctgtc aggcgagtct gcaaacggta
     5401 aatacccact cgagtcagta actacaatgg ctacaatcga caagaacgct caagctcttc
     5461 ttaatgaata cggacgtctt gattcagatt catttgagcg taactctaag acagaagtaa
     5521 tggcttctgc tgttaaagat gctactagct caatggatat caaattggtt gtaactctta
     5581 ctaagacagg tcatactgca cgtttgattt ctaaataccg tccaaatgct gacatcttag
     5641 cattgacatt tgacgaattg acagaacgtg gcttgatgtt gaactggggt gttattccaa
     5701 tgttgacaga tgctccatct tcaactgacg atatgttcga aatcgctgaa cgtaaagcgg
     5761 tagaagcagg tctcgttgag tcaggcgatg atatcgttat cgttgctggt gtgccagtag
     5821 gagaagctgt tcgcacaaac acaatgcgta tccgcacagt acgttaagaa aaatataaaa
     5881 acctatcata tccagcttta gagcttgtgt gataggcttt ttgtatagag ggtaagaaat
     5941 aggcaaaact ttcataatgg attgatactc ttcgaaaatc tcttcaaacc acgtcagcgt
     6001 cgccttaccg tactcaagta cagcttgcgg ctagcttcct agtttgctct ttgattttca
     6061 ttgagtatga aataagatat gcacaaattg attagaaagt caaatgaatt tctacaaatg
     6121 ttttagcaat cgtaatgtac ttgtctagat tcgatctgat atattttcga tttaatgata
     6181 tggtatttaa aacctccaaa gtagcttact ccattctttt acttacgtga gtgtagatgt
     6241 tatttactgt tttagcgttt ttgtgttcca ctctaaccat tatagcattc ttctcagcta
     6301 gtgtactaag gagtgtgtgc ctgaaaatat gggaactaag gggctggttt atcggtttct
     6361 ctagtttagt atttgccttt tgcaaagtga tcttaaatgc ctttctctaa atttacatat
     6421 cactattgtt taacaaaatc taatctatct taggtcactt attctttttt tgaaatgtag
     6481 aatgaacttt ttcaaagttt ttcgaatctt ttaaaatctg tttgttttat atcgccattc
     6541 tccccctttt ttaattctcc ctatatagcc tgacaacttt cccgatggta cgaatatggt
     6601 tgctttcgtc taggtggatg tcggagtatt cgggattgag tttttttgag gtagccttgg
     6661 cggagtttct tgacatagtt agtgccgtct acttggaaga tgccgatggt attatagtca
     6721 atctgtgggg tattcttgat aaataggtag tcgctgtttc ttatctttgg ctccatggac
     6781 ttgctgacga cataagcgat tgggtcgtag tcgtctggga taatggaaac tccataacta
     6841 aatcgtcgtc ctgcatcaag cggctacctg cagagataaa ctacctaaca cgagagtaag
     6901 tagtctgtct gtagtcgtcc agtctgatga tttttacgat acttcgtttt tctgatcata
     6961 cagttgcctc tcggcatagg tcagaacttt accttgtctg ggtggtgccc gttggtcgta
     7021 gatagattgg atatcgctag gagaatcctt ttgaactgga ggaaagaggg catcaatcaa
     7081 gctactgaat actttaacta agtcaaatat agtatttttc ttagtagacc taaccctttt
     7141 ttcataattt ctaatggtgt ttttacttat acctatctta gtacccaatt cttattgagt
     7201 ccaaccatta ctagtctata ttgttttata gttgattgag tttggaatag tacgctgtag
     7261 ctgctaaaac atttctagga attaatttgc ctttcctaat agagttgttc atatcttatt
     7321 tcaatctact atatttttca cctctaacaa tcgcaatctc ttctttatcc atgaacgaaa
     7381 acgctttcta tctttgtaag taaagcatca cacgaaatcc acgaaaatga aaacctttgt
     7441 tgtgttttcg taaaaaattt gttgacagag cacgaaacgc ctactataat taaatcaagt
     7501 ttaaggaaat aacaaaaaaa caaatcggag ggaaagacga tgaacacatt aaatgagaaa
     7561 gtaatcaata tctgtaaagc agtagttaaa gaaactttaa tccaagacat ttaggagaaa
     7621 gaaatgaaga aatcaagaaa attagccaca ttaggaatct gttcagcctt gtttttaggc
     7681 ttggctgctt gtcaacaaca acatgctact tctgagggga cgaatcaaag gcaaagcagt
     7741 tcagcgaaag ttccatggaa agcttcatac accaacctaa acaaccaggt aagtacagaa
     7801 gaggtcaaat ctctcttatc agctcacttg gatccaaata gtgttgatgc attttttaat
     7861 ctcgttaatg actataatac cattgtcggc tcaactggct tatcaggaga tttcacttcc
     7921 tttactcaca ccgaatacga tgttgagaaa atcagtcatc tctggaatca aaagaagggc
     7981 gatttcgttg ggaccaactg ccgtatcaat agttattgtc ttttgaaaaa ttcagtcacc
     8041 attccaaagc ttgaaaagaa tgaccaattg cttttcctag ataacgatgc gattgataaa
     8101 ggaaaggtct ttgattcaca agataaggaa gagtttgata ttctattttc gagagttcca
     8161 actgaggcaa ctacagatgt caaggttcac gctgaaaaga tggaaacatt cttctcacaa
     8221 tttcaattca atgaaaaagc tcgaatgctg tctgtagtct tgcacgacaa tctggatggc
     8281 gagtatctgt ttgtaggtca cgttggggtc ttagtacctg ctgatgacgg tttcttattt
     8341 gtagagaaat tgactttcga agagccctac caagcgatta aatttgctag taaggaagat
     8401 tgctacaagt atttaggcac caagtatgcg gattatacag gcgagggact ggctaagcct
     8461 tttatcatgg ataatgataa gtgggttaaa ctttaatagt gttatgatat gacagtaatc
     8521 ttaggatttt tatttctatt tttgatgagt tggtgggtta tttcaatcgt taatagccag
     8581 aaaccacgca aaaatgaaac atttattggc tatatccaaa gatacgatat tgatgggaat
     8641 ggctatcaat cattaccgtg tcctcagaac tgagaggagt tcttgaaatc gtaaaactac
     8701 tttgaacaag agttacttca acccattggc tccgacggat taagttgctt tcgtgaacac
     8761 caaaatcagc cgcaatttct tcataagtgc ggtattctcg cacatattga agagtggcca
     8821 taagaaggtc ttctaggctt aatttaggtt ttcgtccccc ttttgtgtgt ttaagttgat
     8881 aagcagtttt taatacagct aacatctctt caaaagtggt gcgctgaaca ccaacaagac
     8941 atttaaatcg tgcatcagtt agttgcttac ttgcttcatc attcatagaa ctactatacc
     9001 atattttgtt tcgcaggaag tctaatgcta aaatcttatc agtgagaaga tttcttaaag
     9061 tttattcttt gtagtttcat ctgtattagt acataattta acttagagaa aataggagga
     9121 ctaatcgaat ggaattaaaa gattttacag aaaaagaaca ggaaatgatt aagaaaaggc
     9181 ttacaatgtc taatattagt gacaaagaaa ctactgagaa gattctggcg ctagtaccac
     9241 aagacttgat taagcgaatc ccgttttttg tcagaaaaca tgctacaaca cgtacgatta
     9301 aacgcatttc aattgaacac cctgaactct acgctgtagc tcaaacaagt ggtgagattc
     9361 cagaaaaaaa acgcgaagaa ttgcgtcaga ttatcacaac tatctttgaa caaaagatga
     9421 ataagcatag tattaagtag agaatgaaaa aatattttat tggcggtttg ggaagcaatg
     9481 cctatcatag caaggatttt ctttaagaac tagattcgca ggtctatttt ctaaatccat
     9541 atgaaaagca tcttcgagat gaaacagaat tgaaatcatg gtttaaaaat gagattgtag
     9601 aggaagaatc tatctgtctg ataggtcatt ctattggagg cgatttagct cgttatttcg
     9661 catcggaatt tgaagaagta aagaaactga ttcttttgga tggtggctat ctagatttag
     9721 ataagatttt acctttggat acagagttag aggaaactaa aaattatatc aaatctcaaa
     9781 ttatttcgga cttagatgtt cttacttcta aagaaaaatc tgaagcaaag cattggtcag
     9841 aaaatatgga gaaagctgta agacagtcct atcactggaa tgttgagtat aatagatatg
     9901 agttggctat aaattatgaa aatatagaag cgatactccg cctacggaga aaaatacaag
     9961 cttttaagag agaagtggga gataccttgt ttattagtcc tcgctatcct aatgaagcta
    10021 catggagaga ggaagcccta aaagaattgc cagactattt tgatactatt tttttagaaa
    10081 actttggcca tgagctttat actcaagcac ctaaagaaat cgctagtcta atgaatgagt
    10141 ggctcgctta ttttctatga gctacttgta ttgctggaaa tggtataaga tgaaaatcta
    10201 caagtcacta attttcatat aacaaaaaaa cgaacaggct ctttttctgt tattcagaag
    10261 agaacttgtt cgtttttatc ttgattttct taaaaagctc tatatacata gggattttct
    10321 ggtttatcga ctttggtaaa gctgtcgatt tccaaggttg gaaggttttg tttgagttct
    10381 ttatggtagt gattgaccag ttttcctttg gctgtaatcc aggtgttgtt ttcatactgc
    10441 cgggtattgc ccttggtcag caatccatag acaccagaat ctgcgataca gtggataatg
    10501 ccgaatcgga acagaaattg actattggca tgactggggt cgttatagac aaagcctgta
    10561 aactggattg tcttgccctc aaattcatct ggatagtcgt agatagcctc catgacttcc
    10621 atatagtttt cattagtgat ctgaatacta tcttgggata agtatttatc cgccgccgtt
    10681 cgcatttcct tttcataggc tgattttgaa aaataagaac tagtatctgg tttcaaatat
    10741 tggcttgtag tcccttcact tgtctgaata gctagatccg ttccttccga tagggggaaa
    10801 tgataacctt tagcagaaac ggtctgagaa tccaagctaa cagttgggaa agttaagccg
    10861 atgacaagtg gaatagccag aagagaaata ctcgtcatct tggccaatcg gctgttcaga
    10921 tgactgtggg ttttgacttg cttcatccag atatacaatt gaacaattgc caagataaag
    10981 gaaagcacca tggagatata ggccagatag gaatagtgca tgttgatgta ctggtttagt
    11041 ttgcccgaca gatggaggta aatagtcagt tcaaaatagc cagctaaaac taaaaatcga
    11101 atcataggat gactccaatc aagagagaat aggctaaaac gaccagagtc acaatcgtaa
    11161 tgaagtgact aataaatcgt gctttcaagt aatttttcat catgagaata tttttgatat
    11221 ccagcattgg gccaatgacg agaaaggcca gaactggtgc caaaccgaaa ctcgagagta
    11281 gagaagcacc tataaaggca tccgcctcac tacagagcga aagaagaaag gctaaaagca
    11341 tcaagagcag gatagcaaaa agaggagtcg cactgataga ggtcagaatc cgagtcggaa
    11401 cgtagacctg tattatagaa gcaaagaggc agccaaatac caaataacgc cccgtatcaa
    11461 aaaattcatc aatggcctgc acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag
    11521 aaaaatcatg ctcatgacaa gccagacgat tttctttctg aatcggttct tgccagaaaa
    11581 atcctagaaa aattcctagt attacagcca caagaatgga acccagagct cgtaatagtg
    11641 cgacatggaa ggagttgcca aaggcagaat aggtcgcaaa aagaacaatg ggattgataa
    11701 caggtgctgt cacaagaaaa ggaacggccg tgtaacttgg aacctttttt tccagaaaac
    11761 gattgatgat ggggacgatt ccacattcac aagaagggaa aagtataccg acaaaggtcc
    11821 caaaaaagat tctcccccaa cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga
    11881 tataaacttc aatcagccct gagacaatgc ttcctatcag aacgaaggga agggcttcaa
    11941 tgataatgga gagaaaaatg gctccagttt gaagtacact agatgggagt tgttgaaaag
    12001 cagtcatcta gtttttcttt tctgctttga cttttttctc tttgtcatca gggaaagtaa
    12061 cttgtttcaa atcagggagt ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt
    12121 ttgtaaattt gacagccata ggtgtacctc gattctaaat tctacctcta ttatactatt
    12181 attttgcgga aaagggagtt ttttaatttg ttttcgatag ttgatacact tcgaaaatct
    12241 cttcaaacca cgtcagcttc gccttaccgt agtacggtta ctgacttcgt cagttctatc
    12301 cacaacctca aaacagtgtt ttgagctgac ttcgtcagtc ttatccacaa cctcaaaaca
    12361 gtgttttgag ctgacttcgt cagtcttatc cacaacctca aaacagtatt ttgagcagcc
    12421 tgcggctagc ttcctagttt gctttttgat tttcattgag tatgaaaagg attggagaaa
    12481 gccttccagc ttgtggtata atagatttat ggataaaaaa tatgaaaaaa tctctcagga
    12541 tttgggagtg acgttaaagc aaattgatac cgttctaagt ttgacagctg aaggggcgac
    12601 tattcccttt atcgcgcgtt atcgcaagga catgactggt agtctggatg aggtggcgat
    12661 taaggctatt attgatttgg ataaaagtct gacaaatctc aatgaccgta aggaagctgt
    12721 cttagctaag attcaagaac aaggtaagtt gaccaaggaa ttggaagaag ctatcttagt
    12781 tgccgaaaaa ttagcagacg ttgaagaact ctatcttcct tataaggaaa agcgtcgtac
    12841 caaggcaacc attgcccgtg aagctggact ctttcctctt gctcgtttga ttttgcagaa
    12901 tatagctgac ttagagaaag aagctgaaaa gttcgtctgt gaaggatttg cgactggtaa
    12961 ggaagctttg gctggtgcag ttgatatctt ggtcgaagcc ttatcggaag atgtgacact
    13021 gcgtgctatg acttatcagg aagtgctgag acactctaaa ctcacttctc aagtcaagga
    13081 tgaaagtctt gatgaaaaac aggtttttca gatttattat aatttttcag agacagttgg
    13141 aactatgcaa ggctatcgta ccttggctct caatcgtggg gagaaacttg gtgtcttgaa
    13201 gatcggtttt gaacatgcga cggaccgtat tcttgccttc tttgctactc gtttcaaggt
    13261 gaaaaatgct tatattgatg aagttgttca gcaatccgtt aagaaaaagg tcttgcctgc
    13321 tattgagcgt cgtattcgga cagaattaac tgagaaagct gaagagggag ctatccaact
    13381 tttttctgac aatctgcgca atctcctctt ggttgctcca ttgaaagggc gcgtggttct
    13441 tgggtttgac ccagcctttc gtacaggtgc caagctagct gtcgtggatg caacaggaaa
    13501 aatgctgaca actcaggtca tctatcctgt taaaccagca tcagctcgtc aaatcgaaga
    13561 agccaagaaa gatttagcag atttaattgg tcaatacggt gtagagatta ttgccattgg
    13621 aaatggaacg gccagtcgtg aaagtgaagc ttttgtagcg gaagttctga aagatttccc
    13681 tgaagtcagc tatgttatcg ttaatgaaag tggtgcttct gtctattctg ccagcgaact
    13741 tgctcgtcag gagtttccag acttgaccgt tgaaaaacgc tctgccattt ctatcgcccg
    13801 tcgtttgcaa gatcctcttg cggaattggt caaaatcgat cctaagtcaa ttggtgtcgg
    13861 tcaataccaa cacgatgtca gtcagaagaa actatctgag agtctggact ttgtcgtgga
    13921 tacggtggtt aaccaagtcg gtgtcaatat caatacggcc agtccagctc ttctttctca
    13981 cgtggctgga ctcaataaaa ccatttctga aaatattgtc aaataccgtg aggaggaagg
    14041 aaaaatcact tcacgctccc aaatcaagaa agttcctcgt ttgggggcta aggcctttga
    14101 acaggcagct ggtttccttc gtatccctga aagtagcaat atccttgata atacaggagt
    14161 tcacccagaa aattacgctg ccgttaagga actcttcaaa cgcttggaca ttaaggacct
    14221 gaatgaagag gcccaaagca aactcaagtc gctttcagtc aaggaaatgg cgcaagagtt
    14281 agaccttggt ccagaaactc ttaaagatat cattgcagat cttctcaaac caggtcgaga
    14341 tttccgtgat tcctttgatg ctccagttct ccgtcaagat gtcctagata tcaaggactt
    14401 agtggttgga caaaagctag aaggagtggt gcgtaacgtt gttgatttcg gtgcttttgt
    14461 tgatatcgga attcatgagg acggtctcat ccatatttcc catatgagtc gcaagtttat
    14521 caaacaccct agtcaagtgg tggcagttgg agatttggtg agcgtttggg ttaagcaaat
    14581 tgatactgaa cgtgaaaaag ttaatctgtc tctcctagct ccaaatgaaa ctgactgatt
    14641 acgtcaagca ggtttcacta gaagacttcg gcagatcttt tatccatcat gtccagtgga
    14701 ataggcgtct acgttcgaca ggtgggcgat ttttccccaa agatgggcat ttggatttta
    14761 atcccaaggt ttatcaggaa ctggggttgg acgtttttag gaaaattgtc cgacatgaac
    14821 tctgtcatta tcacctctat tttcaaggga aaggctatca acataaggat cgagatttta
    14881 aggaactgtt gaaagcagtg gatggattac ggtttgtacc atccttgccc aatagtaact
    14941 ccaagccaat caagctctat cgttgccaat cctgccagca aacttatcag cgtaagcgaa
    15001 gaattgatac cagacgctat cgctgtggac tttgtcgagg taaattgcta ctaataaatc
    15061 agcctgagga ctgatgaaag ccgagcctac ccgtgatata ctatgtctaa cttagcagaa
    15121 agaggaaata tcatgaacag taaattttat aaaatgagac gaaatcgcat gatttcagga
    15181 gttttggtag gcctgtcaga caagtggaac tttgatgtta cccttgtacg ttttctgttt
    15241 gccattttta cagtcgcaaa ctttggactt ggggtgatta tctatatcat cctagcttct
    15301 atcatgccaa ccaaggaaga aatcgaagca gaaatgtacg gaacaggccc acgtaaacgc
    15361 aaagaagctc aagccattga cgataatgaa ggctggtttt ggtgaggttt tacaaaagat
    15421 ttggaatacg caaaaagctt taaatcaata gtctaaactg cttgatttaa agctttttta
    15481 cttttatact caataaaaat caaagtgcaa actaggaagc cagccgcagg ctgtaattta
    15541 gtcggcaatg tgaagttaac atagtttgca ctttgatttt tgaatagtat taataatatt
    15601 tcgccagctt catccacaaa ctatagtgga ttggaactag aatagtacac ctctacttct
    15661 aaaatatt
//
LOCUS       DAVLXF010000041        15368 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLXF010000041 DAVLXF010000000
VERSION     DAVLXF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.041"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            118..2283
                     /locus_tag="VYO12_001246"
     CDS             118..2283
                     /locus_tag="VYO12_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1587855.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2283..3023
                     /locus_tag="VYO12_001247"
     CDS             2283..3023
                     /locus_tag="VYO12_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1587856.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3172..4659
                     /locus_tag="VYO12_001248"
     CDS             3172..4659
                     /locus_tag="VYO12_001248"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1587857.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(4703..5980)
                     /gene="ftsY"
                     /locus_tag="VYO12_001249"
     CDS             complement(4703..5980)
                     /gene="ftsY"
                     /locus_tag="VYO12_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1587858.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEAERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5984..6802)
                     /locus_tag="VYO12_001250"
     CDS             complement(5984..6802)
                     /locus_tag="VYO12_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1587859.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVSEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6802..7596)
                     /locus_tag="VYO12_001251"
     CDS             complement(6802..7596)
                     /locus_tag="VYO12_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1587860.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            complement(7593..11132)
                     /gene="smc"
                     /locus_tag="VYO12_001252"
     CDS             complement(7593..11132)
                     /gene="smc"
                     /locus_tag="VYO12_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1587861.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENACKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIVIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11123..11821)
                     /locus_tag="VYO12_001253"
     CDS             complement(11123..11821)
                     /locus_tag="VYO12_001253"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1587862.1"
                     /translation="MKELQTVLKNHFAIEFANKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(12004..12075)
                     /locus_tag="VYO12_001254"
     tRNA            complement(12004..12075)
                     /locus_tag="VYO12_001254"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12040..12042),aa:Arg,seq:ccg)
     gene            complement(12139..13125)
                     /locus_tag="VYO12_001255"
     CDS             complement(12139..13125)
                     /locus_tag="VYO12_001255"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1587863.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVSQLKADTPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13300..14616)
                     /locus_tag="VYO12_001256"
     CDS             complement(13300..14616)
                     /locus_tag="VYO12_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1587864.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14603..>15368)
                     /locus_tag="VYO12_001257"
     CDS             complement(14603..>15368)
                     /locus_tag="VYO12_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1587865.1"
                     /translation="SLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVS
                     PTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKF
                     YIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIR
                     LRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEV
                     ETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY"
BASE COUNT         4402 a         3432 c         2792 g         4742 t
ORIGIN      
        1 attcaagaaa gaaaaggaaa aatcaatgtt atattttctg acacggaaaa tataaaaatt
       61 tgcctttttt tactttttct gatataatga gagaatattc ggaaaaggag actaaaaatg
      121 aagaaaaaat ttctagcatt tttgctaatt ttattcccaa ttttctcatt aggtattgcc
      181 aaagcagaaa cgattaagat tgtttctgat accgcctatg caccttttga gtttaaagat
      241 tcagatcaaa cttataaagg aattgatgtt gacattatta acaaagtcgc tgagattaaa
      301 ggctggaaca ttcagatgtc ctatcctgga tttgacgcag cggtcaatgc ggttcaagct
      361 gggcaagccg acgctatcat ggcagggatg acaaagacta aagaacgtga aaaagtcttc
      421 accatgtctg atacttacta tgatacaaaa gttgtcattg ctactacaaa gtcacacaaa
      481 attagcaagt acgaccaatt aactggcaaa accgttggtg ttaaaaacgg aactgccgct
      541 caacgtttcc ttgaaacaat caaagataaa tacggcttta ctattaaaac atttgacact
      601 ggtgatttaa tgaacaacag cttgagtgct ggtgccatcg atgccatgat ggatgacaaa
      661 cctgttatcg aatatgccat taaccaaggt caagacctcc atattgaaat ggatggtgaa
      721 gctgtaggaa gttttgcttt cggtgtgaaa aaaggaagta aatacgagca cctggttact
      781 gaatttaacc aagccttggc tgaaatgaaa aaagatggta gtcttgataa aattatcaag
      841 aaatggactg cttcatcatc ttcagcagtg ccaactacaa ctactctcgc aggattaaaa
      901 gctattcctg ttaaggctaa atatatcatt gccagcgatt cttcttttgc accttttgtt
      961 ttccaaaatt caagcaacca atacactggt attgatatgg aattgattaa ggcaatcgct
     1021 aaagaccaag gttttgaaat tgaaatcacc aaccctggtt ttgatgctgc tatcagtgct
     1081 gtccaagctg gtcaagccga tggtatcatt gctggtatgt ctgtcacaga tgctcgtaag
     1141 gcaacttttg acttctcaga atcatactac actgctaata ccattcttgg tgtcaaagaa
     1201 tcaagcaata ttgcttctta tgaagatcta aaaggaaaga cagtcggtgt taaaaacgga
     1261 actgcttctc aaaccttcct aacagaaaat caaagcaaat acggttacaa aatcaaaacc
     1321 tttgctgatg gttcttcaat gtatgacagt ttaaacactg gtgccattga tgccgttatg
     1381 gatgatgaac ctgttctcaa atattctatc agccaaggtc aaaaattgaa aactccaatc
     1441 tctggaactc caatcggtga aacagccttt gccgttaaaa aaggagcaaa tccagaactg
     1501 attgaaatgt tcaacaacgg acttgcaaac cttaaggcaa acggtgaatt ccaaaagatt
     1561 cttgacaaat acctagctag cgaatcttca actgcttcaa caagtactgt tgacgaaaca
     1621 acgctctggg gcttgcttca aaacaactac aaacaactcc ttagcggtct tggtatcact
     1681 cttgctctag ctcttatctc atttgctatt gccattgtca tcggaattat cttcggtatg
     1741 tttagcgtta gcccatacaa atctcttcgc gtcatctctg agattttcgt tgacgttatt
     1801 cgtggtattc cattgatgat tcttgcagcc ttcatcttct ggggaattcc aaacttcatc
     1861 gagtctatca caggccaaca aagcccaatt aacgactttg tagctggaac cattgccctc
     1921 tcactcaatg cggctgctta tatcgctgaa atcgttcgtg gtggtattca ggccgttcca
     1981 gttggccaaa tggaagccag ccgaagcttg ggtatctctt atggaaaaac catgcgtaag
     2041 attatcttgc cacaagcaac taaattgatg ttgccaaact ttgtcaacca attcgttatc
     2101 gctcttaaag atacaactat cgtatctgct atcggtttgg ttgaactctt ccaaactggt
     2161 aagattatca ttgctcgtaa ctaccaaagt ttcaagatgt atgcaatcct tgctatcttc
     2221 tatcttgtaa ttatcacact tttgactaga ctagcgaaac gcttagaaaa gaggattcgt
     2281 taatggcaaa attaaaaatt gatgtaaatg atttacacaa gcactatgga aaaaatgaag
     2341 tcctaaaagg aattacgact aagttctatg aaggagatgt tgtttgtatc atcggtcctt
     2401 caggttctgg taagtcaact ttcctccgta gcctcaatct tttagaagaa gtcactagcg
     2461 gtcacatcac tgtgaacggc tatgatttaa ctgaaaaaac aaccaatgtt gaccacgtcc
     2521 gtgaaaatat cggcatggta ttccaacact tcaacctctt ccctcatatg tctgtattgg
     2581 acaacatcac ctttgctcct attgagcaca agttgatgac taaggaagaa gctgaggaat
     2641 tgggaatgga gttgcttgaa aaggttggac tagcagataa agctaatgcc aatccagata
     2701 gcctatcagg tggtcaaaaa caacgtgtgg ccatcgctcg tggcctagca atgaatccag
     2761 acatcatgct cttcgatgaa ccaacttctg cccttgaccc tgagatggtt ggagacgtac
     2821 ttaacgttat gaaggaattg gctgagcaag gcatgaccat gattatcgta acccatgaga
     2881 tgggatttgc tcgtcaggtt gccaaccgcg ttatctttac tgcagatggc gagttccttg
     2941 aagacggaac acctgaccaa atctttgata acccacaaca ccctcgtctg aaagagttct
     3001 tagataaggt cttaaacgtc taaactcaaa ctgtaaggat ttccttgcag tttttctacc
     3061 tcgtattgga atttttgatt tttcggaaaa ttatgttaga attaagttta tgaaatgagg
     3121 tttcctcata cctagcaaga ctaggaataa aaatagaaat taggtagcta gatgtcatct
     3181 aaggttattg ttacaatttt cggtgcgagt ggagacctgg ctaaacgcaa gctctaccct
     3241 tcccttttta gactatataa atccggcaat ctttccaagc actttgccgt tattggaact
     3301 gcccgtagac cttggagtaa ggaatatttt gaatctgtag ttgtcgagtc catccttgat
     3361 ttggcagata gtaccgagca agcccaagaa tttgctagcc acttctacta tcaaagccat
     3421 gatgtcaatg attcggaaca ttatattgct ttgcgtcaat tacaagctga gcttaatgaa
     3481 aaataccaag ctgaacacaa taagctcttc ttcttgtcta tggcacctca gttctttgga
     3541 accattgcca aacacctcaa atctgaaaac attgtcgatg gcaaaggttt tgagcgcttg
     3601 atcgttgaaa aaccatttgg tacagattac gcaactgcaa acaagttgaa tgacgaactc
     3661 ctagcaacat ttgacgaaga acaaattttc cgtatcgacc attatcttgg taaggaaatg
     3721 atccaaagca tctttgcagt tcgctttgca aacttgattt ttgaaaacgt ttggaacaag
     3781 gattttatcg acaatgttca aattaccttt gcggagcgct tgggtgtaga agaacgtggt
     3841 ggctactatg accaatccgg tgccctccgt gacatggtcc aaaaccacac tctacaactt
     3901 ctttcgctcc tcgccatgga caaaccagca agcttcacaa aagacgagat tcgtgctgaa
     3961 aagattaagg tctttaaaaa cctctatcat ccaactgatg aagaactcaa agaacacttt
     4021 atccgtgggc aataccgctc tggtaagatt gatggcatga aatacatctc ttatcgtagc
     4081 gagccaaatg tgaatccaga atcaacaact gaaaccttta catcaggtgc cttctttgta
     4141 gacagcgatc gattccgtgg tgttcctttc tttttccgta caggtaaacg actgactgaa
     4201 aaaggaaccc atgtcaacat cgtctttaaa caaatggatt ctatctttgg agaaccactt
     4261 gctccaaata ttttgaccat ctatattcaa ccaacagaag gcttctctct tagcctaaat
     4321 gggaagcaag taggagaaga atttaacttg gctcctaact cacttgatta ccgtacagat
     4381 gcgactgcaa ctggtgcttc tccagaacca tacgaaaaat tgatttatga tgtcctaaat
     4441 aacaactcaa ctaactttag ccactgggat gaagttggtg cgtcatggaa gttgattgac
     4501 cgtattgaag agctctgggc cgaaaatggt gccccacttc atgactataa agctggaagc
     4561 atgggacctc aagccagctt tgacctactt gaaaaattcg atgccaaatg gacttggcaa
     4621 ccagatatcg cctatcgtca agatggtcgt ttcgaataaa aaaatcctgc aagtttgtac
     4681 cttgcaggat ttttgcttct gattagatta agccttccaa gagacctttc ataaagtttt
     4741 ctgagttaaa ctctccaata tcatcgattt tttcaccaaa accaatcaat tttacaggaa
     4801 tattgagttc ttcacgaatg gctagaacca cacctcctcg agcagttcca tcaatcttag
     4861 tcaaaacaat tcccgttaaa ggtgtgattt tcgaaaattc tttggcctgt actagggcat
     4921 tttgacctgt tgatgcatca agtgccaaga aggtttcatg tggtgcttct ggcacaacac
     4981 gtttgataat acgaccaatc ttttccaact cagccataag gttatcctta ttttgcagac
     5041 gaccagcagt atcaatcatg agaatatcga taccttcagc cacggcacgt tccataccat
     5101 caaagaccac gctggctgga tcagcttttt caggtccagt tactactgga acatctactc
     5161 gtcggcccca ttcagctagc tgagctactg cacccgcacg gaaggtatct gctgcaacca
     5221 gcatgacctt cttaccagct cgtttgtagc ggtgggctag ttttccgata gaagttgttt
     5281 tcccaacacc attcacacca acaaagagca taactgtcaa gttatcttgg aagtggatgc
     5341 tttcatcgta gctaccatcc ttttcataaa gctcaaccaa tttctcaatg atgacacgac
     5401 gaagtgcatc aggtttcttg gcattttcaa gcttggcttc gtaacgtagt tcctccgtta
     5461 agttagaagc gacttggaca ccaacatcac tcataatcag cagttcttcc agttcctcga
     5521 aaaattcttc gtcaacagag cggaagttag caaagaaggc attcaagcgg gcaccgaaac
     5581 ctgtacgagt tttcttaaga ctgcggtcat atttttcctg aacggtttct tctgcttgag
     5641 gcctttctgc ttcaagaact tcagaattat tttcttctat agtctctaga aactcagtat
     5701 tcccttcttc tgagtgtgga aattcttcga cttctttttg agaaacttct acaactggct
     5761 ctaaatccag actttcttca actgtgtctt ggatttcctc ttcttggaac acagcttgtt
     5821 caacaatttc aacctctgct tcttcctgag aaacttcctc aacttctgtg aaggtaggat
     5881 caacatcttc agacaaatca agattttcca gagcttcttt tacaacttct tcgattttag
     5941 gttcttcttt ttttccgaat agacggtcaa acaatcccat atcttagttc tcctttagca
     6001 catattcttc gatagcccag gcgacagctt cctcatcgtt ggtcatcggc gtcactacat
     6061 ttgcggctgc ctttacttca gaaacagcgt tttgcatagc aacaccaaga cctgcccatt
     6121 caatcataga gaggtcattg gcctcgtcac cacaagccat cacttgactt tggtcgattc
     6181 caagatggct gattagtttt gccaaacctg ttgctttatg aacattcttt ggtgaccatt
     6241 ctagcaacat ttcacgtgat ttaaagattt catattggtc aaacaattct ggagaaatct
     6301 tctgaatggc tgcatccaag ggttcttgag caaaggcagt cacgcatttg ttgtaggtca
     6361 tttgactaga taagtcttca aagtccactg gaacaaaggt caaagctgga ttgaatttgg
     6421 cataaagact ttcttggtcc gattggattt gataaactgt tccttctgag atggcatcaa
     6481 gaggcagtga taatttctct gtttcttcat acaaacgtgc cacatcatca tatgaaaaga
     6541 ctgttttatc aaggatttct cctgtatttt tctgaactaa tccaccatta aaagtaatgg
     6601 tatactcatc ttcatggccg tcagtcccta actcatggag aaagaagtcc atggctttta
     6661 agggacgacc agttgtcaat acgaccttga taccacgatc acgcgcagct ttcaaggttt
     6721 ccttggtacg atccgtcagc cttttatcag tagtcagcaa ggtcccgtcc aagtccaatg
     6781 caatcaattt tatatctgcc attataagcc ctccatataa gctataaccg accgttcctt
     6841 atggtgacca atcacagtct ttgctaattc taaaatttca ggtcgtgcat tttcaggagc
     6901 tacaggatgt cccacaacct gcatcatatg taagtcatta agattgtctc caaaagccat
     6961 gacctgatcc attgtgatac caagtttttt agctaattca acaatagcta cacccttatc
     7021 gacatagtcc agaacaatat caatagattc aaagccagtt gtcatggcct taacaccagg
     7081 gacatgttca ttaacccaag cttcaccagc ttctagcgtt tcttctgtga agttggttgt
     7141 aaatttgaaa atgtcatctg tgatatcttc caaactcgct actttttgga tattttcatt
     7201 ataatgctga ctcactttca aataggtctc atcaaccgta tctagaacat atgaaccctt
     7261 cttacccgtc aagagcagtt tattgatatc tacataaggt gacgttttca gcttttcaaa
     7321 agttgccaga taaaagtcac gagacatagt cgcttcatac aagtcctgac cttgatactc
     7381 taccaaactg ccattttccg cgatgaaaat aatgtcatca cgaacatcag caaataattt
     7441 ttccagagac agaaatcccc gacccgaagc caccgcaaag taaatccctt tttccttgta
     7501 ggaagccaag agagacttga gacgatccat atcaaagcgt ccattcccat ctaggaaggt
     7561 tccgtccata tccgttgcta ctagtttaat tgtcatcctt caatactttc taaatctttt
     7621 aacttaactg aaacaatctt tgaaacaccc gattcttgca tggtcactcc atagatggaa
     7681 tcagccgctg ccatggttcc cttacggtgg gttacaacga taaactggct gtccttgtca
     7741 aagcggttga ggtaatcccc aaaacgttta acattggctt catccagcgc agcttccacc
     7801 tcatccaaga tgacaaaagg aatggtcttg acacgaataa tggagaaaag caaggcaaga
     7861 gccgatagag ctttttcacc accactcatg aggttaagcg actggatttt cttacctgga
     7921 ggttgaacag aaatctccac accagctgtt aaaaggtcgc cctcagtcaa tatcaagtct
     7981 gcctgacctc cgccaaacat ctgcttgaag gtcactttaa aggactcacg aatagcttca
     8041 aaggttgatt taaagcgttc cttgacctca tcattcatct ctgtaatggt ttcaaggagc
     8101 agatttttcg ctgacaaaat atcatctcgc tgactattta gaaagtccag acggttgtga
     8161 acttcttcgt actggtcaat agcttccaag ttgacaggtc ccaatgagcg aatagccttc
     8221 tctaaatcct gcacctcttg ctctgccaga ttgaggtttt ccaattcatg tgccttttct
     8281 agtgcttcag tatagctaat ctggtactgg tctgttaatt gattttgtag atggcgcaag
     8341 cgctcgctga ccttttcttt cttggcttca gcacgtgttt gcttgcgaat ccactcctca
     8401 ttctgctggc gagcttgatc caagtgactg gcaatatcat ccagttgacc ttctatatca
     8461 tccaactcaa actgcttgcg aatcaaacct tgttggagat ttgttttctg agttttggat
     8521 tcttccgcct gttgactgag caattctgta tcaaccttct caagattgtc aactttttct
     8581 tggagcatgc gctgaatttc ttcttgttcg atattaagat tgtccaattc cttgcctaaa
     8641 cgctcaatat cagctacttc ataacgtttt tgcccttgca gttctgtctt aagcaagcga
     8701 gcttgcgcca cctcttcctg caaattttga tagcgttctt ggatggcgtt tttattagac
     8761 ttaatttctt caatctcagc ttccagattt tgcttttcac tggcgatagt agcaaggctc
     8821 tcttgacatt tttccttatc cgcttgccaa tctccctcag aaagacgatc tatttcctct
     8881 tcttggagtt tccaaagagt ttccagttct tcaacttgct gactagtttg ctgataagcg
     8941 agggacaagc cttgctcctg aatacgtgcc tgctctcctt gagatttgat ggcttctaat
     9001 ctttctgtca atgcagccat ctggtcttgc aaggtcttca aagccgcttc ttctgaaccc
     9061 aagcttgctt catctgcagc aatttctttt tgtaattgct ccagttctgg cttgatgaca
     9121 atactgttat tttggcgatt ggcaccaccc gcataggaac cacccgtgcg taattctgtc
     9181 ccatccaatg tcaccatacg aacctgataa cgaacttgtc gagcagcttc acgcgcatgt
     9241 tctacggtat caaagatagc cgtcgtagct agcaagttct tgaaaatggc ttccagtcta
     9301 gtatcaaaag tcaccaactc atctgccatc ccaaggaaac ctgggcttac agcgatagca
     9361 tcttggttct gactagaaat cgtacgcgcc ttaatagtgg tcaaaggaag aaaggttgca
     9421 cgaccggctc tgttccgttt aaggaagtca atagccttgg ttgccgcatt ctcatcttct
     9481 acaataatgt gctgactact cgctccaagc gcaatttcta gggcagtttg ataatgcata
     9541 tcaaaggtca gatgctcact gactgcacca ataatcccac ctaggcgatc tttttcttgg
     9601 agaacactct taacacctgc ataaaagtta ctatgatttc tcaggatatt ttccaaactt
     9661 tgagctctgg cctgcttgtt tttgagatta tccagacggt caaagagttg gctttgttga
     9721 gcttgatagg aagttttctg ctcctcttgc tccttggcaa tagcttggta gtcagccaat
     9781 aatttctgaa cctgctcctt ggcagtttca agctcttcct tttgctgact agccttctct
     9841 ttagctatag ctaattgctc tttcagcttt tctagttgat ctgcttgttt ttgagaaagc
     9901 tgacgactat tttccaactc attctcaata cgggtcaact ggtttgagac atccgcttct
     9961 tcttgtaaaa gagctacaaa gcgttcacgt aagagctcaa tcatctgatc aggatcgtct
    10021 gagaaagcca gcaattcagc ttctaaacga ttgagttttt gattattttg gactagattt
    10081 ccctctaaca gagctaaaga gctttcttta tcagactttt ctttgctgag tgaatttctc
    10141 ttatcctcca aagcagccaa acgagcttgt gcctcctgtt gattcaaggc cacttgctcg
    10201 gactccagtt tcgatagggc taattttctt tctaaatcac taatcagact agtcaagtcc
    10261 atcaaactgc cttggtcttt ggccatttca gcctgtaaat cttggcgttg ctttttaaga
    10321 gtttgatttt cttcttctaa tttttcacgc ttttggtaat aactcatcaa gagttcttga
    10381 acctgagcca gctcttcttc tgtcgactct agttctgcct tattttcctt gatttgagca
    10441 accagaacgt ctaaataaat agccttacgt tgtccttcca agtctaaaaa cttacaggca
    10501 ttctcagctt gcttctcaag aggcttgatt tgattatcca actcgtagat aatgtcctct
    10561 aagcggtcta gattatcctg agtttgttgc agtttactct cagtttcttt tctgcgagtc
    10621 ttgtatttta aaactcctgc agcttcttca aaaatagctc gtcgttcctc aggcttggaa
    10681 ttaaaaatct cctcaacctt cccttgggaa ataatagaga aggaatctcg tcccaatcca
    10741 gtatccaaga agaggtcatg aatatcacgc agacggactt tcttgccgtc aatcttgtat
    10801 tcgctatctc cactacgata gatatggcgt tccaccctga tttcttgacc tgcatccttg
    10861 ataaatccgt catgattatc cagagtcaca actacagaag cataattgag cggtttgcga
    10921 ctttcggttc cagcaaagat gacatccggc atcttgcccc cacggagact cttgacacta
    10981 gactccccca aagcccaacg cagactttct gtaatattgg actttccaga tccattgggt
    11041 ccaacaactg ccgtcacacc ttggtcaaaa acgaccttgg tcttatcagc aaaagacttg
    11101 aacccctgaa tttcgatttc ctttaaatac atgaatccag ccccttctca acggcatttt
    11161 tggcagcttc ctgctctgct aatttcttag aacgaccttg gccttgaccg atgctcttac
    11221 cttcaacaag aacttctaca tcaaagacct tatcgtgagc aggccctgtt tcagaaatca
    11281 cctgataacg aatagccaca tcaccattga cctgaagtaa ctcttggaga tgggttttat
    11341 agtctgtaat catctcaaat tcgcctgctt caaccttagg aatcatgact tgatagataa
    11401 attccttgac cttggccaca tccttatcca aaagaagggc accaagaaag gcttcaaagg
    11461 catcaccaag aatggtgtca cgattgcgac caccagattt ttcttcccct ttacccaact
    11521 tgataaactg gtcaaactgg caatcacgcg caaaaccagc taaactctcc tcacggacaa
    11581 tcatagcacg gagttttgat aggtcacctt caggcttttt aggatatttt ttatatagat
    11641 attctgaaat caataactgt agaacagcgt ctcctaaaaa ttccaagcgt tcattgtgtg
    11701 aaatttttaa gaggcggtgc tcattggcat aactcgtatg agtaaaggca gtttccagta
    11761 aatttttgtt tgcaaattcg attgcaaaat gattctttag tacagtttgt aattctttca
    11821 taccaacctc tttctaactg ataatagtcc tttttattat atcaaaaaaa gccccctgag
    11881 tcactctaaa acgggactgg aaagcatttg ggaattcttt agacagagat tctcagtttt
    11941 agcggcaaat ttgggtcagg ataaagaaaa aagccctatt aaaggctttt taggatgttt
    12001 acatccaccc tgagggaatc gaacccccat ctcaagaacc ggaatcttac gtgatatcca
    12061 ttacactaag ggtggaaact tgttttatta taacagaaat ttgctctaat aacaagtttt
    12121 ttggtcaaag accccgtctt agtgggaagc atccccattc cagatggagt ttttcacgat
    12181 cacataatca acgtgtttaa ggtcagcaac ctgacgtcca cctgcataag aaatagcact
    12241 ttgaaggtct tgttccatct cagttaaagt gtcttgcaga tgacctttag caggaagcaa
    12301 gatacgtttg ccttccacat ttttgtaagc acctttttga tattgtgagg ctgaaccata
    12361 atattctttg aactgttcac catcgacttc aatcgttttc cctggacttt caatgtgtcc
    12421 tgcaaagagg gaaccaatca tgatcatgct agcaccgaag cggatagact tggcaatatc
    12481 accgtgagta cgaattcctc catcagcgat aatcggttta cgcgcagcct tggcacacca
    12541 gcgtagagca gccaactgcc aaccacctgt accaaaacca gtcttaacct tggtgataca
    12601 aaccttacca ggaccgattc cgaccttagt agcatccgca ccagcatttt ccaattcacg
    12661 cacagcttct ggtgttccca catttccagc aatgacaaag gtatctggca attctttctt
    12721 gatgtgttga atcatagaaa tcacgctatc cgcatgacca tgagcaatat caatagtgat
    12781 atactcagga gtatcagcct tgagctggct aacaaaatca tactcataat ccttaacacc
    12841 gacagagata gaagcaatga gcccttgatt gtgcattcgt ttaataaaag gaatgcgtcc
    12901 tgcctcatca aaacggtgca taatgtagaa gtaaccacct ttagccagtt gctctgctac
    12961 attttcatcc aaaatcgtct gcatattcgc tggcacaaca ggtagtttaa aggtgtgatt
    13021 tcctaaagtg acacttgtat ccgcttctgc acggctttta atgacacatt tatttggaat
    13081 caattgaata tcttcgtaat caaaaattgg aaattcattt aacatatcga tgtctcgttt
    13141 cttttgtaat gacctaccta tgctcttgca tcactacgcc ttttccgacg tttccttaat
    13201 ttattataaa tcaaagtaca gtttttgtca aattatttta tgaattaaaa tatatcgttc
    13261 gggttttact tgtgttaaaa ataaaagaaa gaggggaaat tattttctcc ccacttcttc
    13321 atcaataatc gctttaaaat tttcttctga attttccatc attttacaaa atgtactata
    13381 gaatgaggca ttctgaggga ttcgaataga ccacttcttc aattgagctc catagccagc
    13441 tacttttcct atttcactat ttactgactg ctccatacta ggaaaaatca gtccagcctt
    13501 ctcagctttt aaaatataag aataggaaat cagctggaat aagtcctcac ggttgattcc
    13561 tttttcagtc aattccagtt ttttatattt tgcatctaga acaatctttc gttctctgtc
    13621 ataaaaatct ggatatactt ttcgtttccc aacagaaaat actgaaattc catccgtctt
    13681 atccttattt ctgggatgta caaaaccttt tggcaacaag gtgtaaacat actcttccca
    13741 aagccaggca acatcaaaga gaataccata gattttttga tcttgatacc ctaaaccgtg
    13801 cttttcttgg tttaggatca tcagacaaag ttcttgtaag tttctgtact cgtgaaagta
    13861 tgcatgacgt ataggtttag attgatttcc ccgaataatc ttagcacgat cagctagttt
    13921 ataagagggc gttacacgca cgatttcaga tacgttttca cgactagttg agagattatc
    13981 tagtacccct tgaccaatgc ttttctgatt cttaatgtat tcaatagtgt gacggaccaa
    14041 ctgcatgagg ggattatcat aggtgaactc tctcgttgcg taggcaatat ttcccgtgaa
    14101 aggaagattt ttcttgagat ggtttcttac atcaatcact cccttaacat gactgtcgtt
    14161 atgagaaaat ctctggtatt ccttataaag accttttcga atagcagctt gtagatactt
    14221 ggggaagaga tacatcaaaa gttgataaag cctctcttca cgagacaaag caacatctaa
    14281 actagtgaga ttgatatgaa gaaccttgtt taagagataa tgcaaaaaat ggtcattact
    14341 ctcatcagaa aatcgtgagg aaatcgttaa tctttcctga ccatatccaa gaaaaccaat
    14401 cacgttccct gtcttgattt tctgattgac tgtttcaaaa atcttttggt ccttatccaa
    14461 atcaggagta tgagtcaaat catttggaaa aatgaaaata cgttcatctt gggatagact
    14521 ttccaaggtt ctgtctaaaa gaagattgct tagtttagga tattcttcaa caaatttttc
    14581 tttaataatc ttgtgttgat tatcagtaat ccgcatcatc gttttcatcg ccttcattgt
    14641 catcagctac tgcctgatct ttttgctcat tatttgtcag ttcaaatgct tttttcaaag
    14701 tttccaaagt ttcaacctca tcataagaac ctcgcaagta gtcttctagg agaggcttaa
    14761 tataatcaga ccagagtaat tcatagtcaa aatctacatc cttcaactta agaaaataac
    14821 ttggtccaat atgataatga ctgtttaatt cctgaacatt ttcgatagca gcgttcaagt
    14881 ttcttagacg aatttttgct tcttctgcat ggatattcag ctctttatcc aacatgccaa
    14941 cttgaccctc ggcagtaact tcaacaaaac gaaaacgacg acgcatagca aaatcaaagg
    15001 tatccactga acgatcaata tcattcatag ttccgatgat gtaaacattt tcggggatat
    15061 agaacttttc atcagtttcg tgtagatttg catattgggt agaaacactt cctttttcac
    15121 cacgatagcc agggtcgata gagaaaaaga gttcgccaaa aatcttagaa atctccccac
    15181 gattgatttc atcgatgatg aagacgaatt tcttatcagt atcaatttct gttggggaaa
    15241 catagtcttt caaaccaaat ctctttctca atgtttctag gacagttttt ccaccacttt
    15301 tgtagtattc ttgcttatta taatttttat ctttatacaa ctcgtaaaca tatttgctag
    15361 gtagtgac
//
LOCUS       DAVLXF010000042        14776 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLXF010000042 DAVLXF010000000
VERSION     DAVLXF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.042"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(120..1019)
                     /locus_tag="VYO12_001258"
     CDS             complement(120..1019)
                     /locus_tag="VYO12_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1587866.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(1091..1651)
                     /gene="rnmV"
                     /locus_tag="VYO12_001259"
     CDS             complement(1091..1651)
                     /gene="rnmV"
                     /locus_tag="VYO12_001259"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1587867.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(1651..2424)
                     /locus_tag="VYO12_001260"
     CDS             complement(1651..2424)
                     /locus_tag="VYO12_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1587868.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPMIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIRLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(2729..3622)
                     /locus_tag="VYO12_001261"
     CDS             complement(2729..3622)
                     /locus_tag="VYO12_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1587869.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEVTPEY
                     KLETTPGGDKGGNIGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRLVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(3906..4040)
                     /gene="rpmH"
                     /locus_tag="VYO12_001262"
     CDS             complement(3906..4040)
                     /gene="rpmH"
                     /locus_tag="VYO12_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1587870.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(4185..5399)
                     /locus_tag="VYO12_001263"
     CDS             complement(4185..5399)
                     /locus_tag="VYO12_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1587871.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(5555..5848)
                     /locus_tag="VYO12_001264"
     CDS             complement(5555..5848)
                     /locus_tag="VYO12_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587872.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            5997..6449
                     /locus_tag="VYO12_001265"
     CDS             5997..6449
                     /locus_tag="VYO12_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1587873.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(6484..7872)
                     /locus_tag="VYO12_001266"
     CDS             complement(6484..7872)
                     /locus_tag="VYO12_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1587874.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELHRQL
                     KAYERHKERNKS"
     gene            7938..8900
                     /locus_tag="VYO12_001267"
     CDS             7938..8900
                     /locus_tag="VYO12_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1587875.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HTLDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLTMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(8897..9088)
                     /locus_tag="VYO12_001268"
     CDS             complement(8897..9088)
                     /locus_tag="VYO12_001268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587876.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHVFEVVDRTDEVS
                     NHTYGKATLTWFAEIFEEY"
     gene            complement(9152..10162)
                     /gene="ccpA"
                     /locus_tag="VYO12_001269"
     CDS             complement(9152..10162)
                     /gene="ccpA"
                     /locus_tag="VYO12_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1587877.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(10587..11186)
                     /locus_tag="VYO12_001270"
     CDS             complement(10587..11186)
                     /locus_tag="VYO12_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1587878.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVAVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(11188..12285)
                     /locus_tag="VYO12_001271"
     CDS             complement(11188..12285)
                     /locus_tag="VYO12_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1587879.1"
                     /translation="MLERLKSIHYMFWTSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMCEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     DSMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(12285..13022)
                     /locus_tag="VYO12_001272"
     CDS             complement(12285..13022)
                     /locus_tag="VYO12_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587880.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGDINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(13024..13908)
                     /locus_tag="VYO12_001273"
     CDS             complement(13024..13908)
                     /locus_tag="VYO12_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587881.1"
                     /translation="MDMIKVQGLHKNIKGKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELVAFFQSIY
                     PNPLSNQEIDQLLQFDKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVQDLKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(13895..14089)
                     /locus_tag="VYO12_001274"
     CDS             complement(13895..14089)
                     /locus_tag="VYO12_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587882.1"
                     /translation="MKKAFLYLFIGLSLVVWLVEMFTGWFDQALLRQFIRGAWGLGFM
                     IFIAFPMGMEWLKGEYHGHD"
     gene            complement(14086..14277)
                     /locus_tag="VYO12_001275"
     CDS             complement(14086..14277)
                     /locus_tag="VYO12_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587883.1"
                     /translation="MKNKMIVAMSLVAAGVMTYLMFSGLDEDFYHFPWELFAGFGMMS
                     WLIREGLKLVSDVKKEFEK"
BASE COUNT         4368 a         3376 c         2631 g         4401 t
ORIGIN      
        1 ttaaaatcta actatttcat attattttta gcttatatct aaaaaaacag ttctaaagac
       61 gaactttaga actgttttga tacgaggaca aggacatttt tgtcactgat tcactatgtt
      121 tataaatatt ttttgacatc ctcttgccat tcctctgtca atttctttac tagaaatcca
      181 tctccaatta gcttcgcaaa tgttttagaa gactgataaa atgattcagc taaactaaaa
      241 tctctttcta ctcttaatgc ttgcttccac aaaaacataa atacaagagg tttcttttga
      301 taatcttgag ttttatccat aatttcttgt aaacacgata gataatcatt aaaacaatca
      361 gttactccta ccttttcaag acaacacatt cctgaaataa gagtatctct aattataaag
      421 taattctcta tttgagttac cttcttctgt tccaaaagaa agagcattag cttttcgatt
      481 tgatttataa atttggcaag atcttttcta atgagcattt gataaaaaaa caaacgaatc
      541 aacaacatat catttattga ataagcttct ttttctataa tctgaggcat atgttcctca
      601 agtattaact ctgcatattc aggccttcta gttctagaag tatccaaaca cgcctgcaat
      661 acttcacatg ctaattgttc ttctttagga agattatcaa agtatgtttt ataaatctct
      721 tccaaacacg cttccttctt atcgtactcc tcttctttac catagattgg ttcacgtaaa
      781 atctggtatt tcaattctaa ataagcagaa ggaagagctg aaaaatccgg cataaggcta
      841 taaactggaa ctcctagccg gcgagcaata taggctaact tgggcaaact cggttgcgaa
      901 gctccacttt caattcgcga cagttgacga actgtcagtt cttgctcatc tccacaaaaa
      961 tctggacgac taattccttt ttctatccga atctctttaa ttttttgacc aattaacatc
     1021 agacgcctcc tcttctacct gcttcgaaaa atactgtgaa acttaaaaaa ttgtaacggc
     1081 tacatctttc ctactcataa gatttcatag cttcttccac ttctgccaaa gtaaccccaa
     1141 acaactctag gcgtttgagg agttgcttgc cgttagaata gccgattcgg agagtctctc
     1201 cgagatattc tctacgctta cggctgtctg cccctgctag aaaaccaagg cgaatcaaat
     1261 cgctacgact aatgtcaaac tgactctcat gttcaaattg ttctgtcacc tgagctagag
     1321 ccgttttcag gtcttcatag ctggcatgct caattcccag agaacgcccc ttggtcttgg
     1381 acttgggaac agcttcatct cgcttgagaa aggcatgctg aactgttgga atggccgtca
     1441 taatcatgcg ccgaatccgc tccccattaa aatctgggtc tgtaaagaca atgactccat
     1501 gacgttggtg tagacgctga atccgctcta tgtcctggtc attgatggca gaacctcgag
     1561 tctcataggt ctccacatcg aaataacgct tgagattgac cgtatcatca cgaccttcaa
     1621 ccacgataac ttgggaaatt ctctctttca ttacttgctg tccaatccaa aaatgcgttc
     1681 tgcatttgca gtcgttgcta ccgccagctc ttctgtcgtc ataccacgca agtcagcgat
     1741 aaagtcgacc acatagcgag tataggctgt tttattttca cgaccacgct tgggtacagg
     1801 tgctaagtaa ggcgcatctg tttctaccaa catcttgtcc aaaggtaact ctttagctgc
     1861 ttcttggagg tcagttgcct tcttgaaggt caccactcct gagaaggaaa tggtcatacc
     1921 aagatccaca aacttctctg cccactcaag cgtccctgaa aatgaatgca tgataccacc
     1981 acgaggacca acgccctcac tcttgataat ctcataggta tcttccagcg catcacgggt
     2041 atggacaaca aaaggcaaat ccaagtcctt agatagccga atctgacggc gaaaaacctg
     2101 ctcctgcacc tctttgggcg ctgtcatcca atggtaatct aagccaattt cacctaaagc
     2161 cacaaccttg ggatgtttta acttatccaa caagtaagcc tcaacttcct ctgtataagt
     2221 accagcttct gtaggatgcc aaccaatagt cgcatagagc tgctcatact catctaccaa
     2281 ctccaaggca cgctcaatca tcggtttatc aaaaccaaca atattcatct gtatcacacc
     2341 catctcagca gccaaggcaa tttcttctgc ctcacgacct gcaaattctt ctacattcaa
     2401 gtgtgtatgt gtatcaaaaa tcatctcttc taacctcgtt ttctatcttc tattatacca
     2461 aaaaagagga ggggcaccta atttttcggt ttcccctcct ctcttcaata gagagctatt
     2521 ctgctatctt ttctatccga tattgcccat ctcctattcc acagttagag acagaagaga
     2581 ttggctacat tgatagaata ttcctattca gctaaagtat gtgattatct tgggttctca
     2641 cttttctcac ccatcactag gattgatgac ttctcaaaat cccccttata atctaacttt
     2701 aagtagagaa tagaattttc tagctgttct attttctacg tctagtcttt cttcctagca
     2761 aagcaaaacc acttgttgcc accactagac caagactagc aagtgctgca cttgatgctg
     2821 aacctgtgtt aggtaattgt ttgccagcag gtgactggta ggtaacatct tgcattgttc
     2881 ctgcagttgc ttgcggtgca cttgttgtct taggagcagc attaggagcc actagtctct
     2941 taggaactgt aaccatggca atggcttgca ctccaatcgt ttctgcttcc ttcacatctt
     3001 ccatagtttt cgcattttca atctctttga gagctgcttg tttttctgct tccactcttg
     3061 ctaaaagttc tgctttttct ttatcagaaa gcggtgcgcc tttgatttca tcctgcttgt
     3121 ccttggctgc tttttcaatg gcatttttag ctgattcttt ttcagtagct aattgcttag
     3181 aagcttgtga ttgagctgag ttttgtgaac ctgtgtgagc tgatccaccc gcctggctac
     3241 caccgccttc attcgcatta gcatctgagc ttccaatatt gcctccctta tctccacctg
     3301 gtgtagtttc tagcttatac tctggtgtaa cttcttgagt tacagattca cctttcgcag
     3361 attgttgaac tgtatctttt cctagaatcg tcactactga accatctggg aatgtgattg
     3421 ttgctgtacc atctccagct acattgattg tcgctccgtc taatgctgaa ccatttgctt
     3481 gtaaaatagc aaccttaact ttttcttcat ctgttaaatg agttatatct tttacatcta
     3541 ctctttgagc aggtgcttta acagttgatt tttcagcagt cggtttagtg ctatcttgtg
     3601 cttttggatc ttgtgctacc aattgatttc ctggaattgt aatagctgaa ccatctttaa
     3661 agacgattgt tgctgtacca tctccaccta cttcaacttt atagtaaaat aaaataataa
     3721 caggacaaat cgatcaggac agtcaaatcg atttctaaca atgttttaga aacagaggtg
     3781 tactattcta gtttcaatct actataacat cgcttccgaa tccatttgca tctactgaac
     3841 ttttttacaa aaataaaaat cagtagactc gagttcctac tgatttttga tagaattcgt
     3901 ttggattatg cagccaaaac tttgcgtcct ttacgacgac gagctgccaa tacgcgacga
     3961 ccatttttag ttgacatacg gttacggaat ccgtgtttgc gcgcacgacg aagtttactt
     4021 ggttgataag tacgtttcac gatgaatacc tcctcataga ttttgtattc gtttagccgg
     4081 ctataaagtg atcagttact aaacatactt tactattcta tctgattctt tcacttttgt
     4141 caatagctct aggcaaatgt ttccagcttt ttcgaatgca agccctatct acgatactgt
     4201 ttcaagaaac gagtcatctt ttcttggatt tgggctaact catcaacacg aggaaggtaa
     4261 acgatacgga agtggtctgg ttcctgccag ttaaagcctc gaccatgaac caagagaacc
     4321 ttttcctgct tcaagaaatc aaggacaaac tgctcatcat catcgatacg gtacatattg
     4381 cggtcgattt ttgggaagat atagagcccc gccttgggtt taacggcaga caaacctgga
     4441 atatcttgaa tggcattata gatgaaattt ctttgctcgt agattcgtcc accaggaaga
     4501 agcaattcat cgactgattg gtgacccccc aaggaagttt gtacgacttg ttgggccaaa
     4561 acgttagagc aaaggcgcat attggacagc atattgagcc cttcgatata gcccttaaca
     4621 tgagtcttag ggccagacaa gaccatccaa cccacacgga aacctgctat gcggtgggat
     4681 tttgacagac cattcatgct gacacagaag acatctggtg ccaagctcgc cacaggcgta
     4741 tgcacatgtc cgtccattac catgcggtca tagatttcat ccgcaaagat aatcaaatcg
     4801 ttttgacggg caatctcaat aatctccagc aagagttcct taggataaag ggctccagtt
     4861 gggttatttg gattgataag gacgattgcc ttggtattgg aagtgatttt tgacttgata
     4921 tcgtcaatat ctgggtacca ttcaacagct tcatcacaga tatagtgaac ggcatttcct
     4981 ccagctaggc tgacagccgc cgtccataga ggatagtctg gcattggcac taagacctca
     5041 tccccattgt ccaaaagccc ctgcatggac atgacaatca actcactgac accatttcca
     5101 aggtaaatat catcaatatc tacgtttggg aatttcttca gctggcaata ctgcatgatg
     5161 gccttacggg ctgagaaaat ccctttggag tcagaatagc cttcactatc acgcgcattc
     5221 ataatcaagt catgaatgac ctcgtctggc gctgtaaagc caaattctgc tggatttcct
     5281 gtattcagac gtaaaatctt ttctccattc gctcgcatcc gcatggcttc ttccaataca
     5341 ggaccacgga tatcataagc aacatgctct aacttactag acttgttata ttctttcatc
     5401 ttgtttcctc aattcatcag gatagtaaca ttataccact agaaagagaa tgcgacaagt
     5461 ttactgaaat gtgttactag tttcgtatag aagaaaaacc ttgctaaagg cgaggttaag
     5521 aacgtagaga tagcaggtat tttgcaaaga cacatcacat accaaaagat aaacctagaa
     5581 aaagcaagcc tagaaaggag tcataaagaa gattaaacat gaggaaaagc ccccatatca
     5641 tcaagtaatc ccaacgccca cttcgttttg caactagaaa taagattaga tggtagagta
     5701 catgaaagac taaaaatcca ataaaacttg gataaagaag cggtaccaga ctctctaatt
     5761 gacagatcat gatcactttt accaagactg aaactaggat aattccataa taaaggaaat
     5821 ttgatttttg aaaagagttt ttcatcattc tcctttcctc ctatcctttc ttttatttta
     5881 tcatatattt atatagagtt taagccctaa tattcttttg aaaaatactt tttttgcttt
     5941 acttctatag tgaaaaggtt tacaattata gtaagaaaat ttcaggaggt ttcattatgg
     6001 cacaacgtta ccaaaatatc atggtcgcaa tcgatggttc taaggaagcg gacttggctt
     6061 ttgtcaaggg agttcattct gctctacgaa acgacgctaa actcaccatc gcacatgtca
     6121 ttgacacacg cgctctccaa agcgtatcca cctttgatgc tgaagtttac gaagaactcc
     6181 aagtcgacgc tgaaagtctg atgaaagagt acgaaaaacg tgctaaagat gctggggtag
     6241 cagatgttca tatcgtcatt gaaatgggaa atccaaagac cctgctagca cgtactattc
     6301 cagatgccga ggaagtggac ctcatcctcg tgggcgcaac tggtctcaac gcctttgaac
     6361 gcctcttggt cggctcttca tctgaataca tactccgcca tgctaaggtc gatttgctgg
     6421 ttgtgagaga acaagaaaaa accttataat cacaaagaaa aggagcccct agctcctttt
     6481 tgtttacgat ttatttctct ctttatggcg ttcgtaagcc ttgagctggc gatgcagttc
     6541 ctttttaata gcaggttctg gagcatattt ttcttcccaa ttatctggtt ttaagatttt
     6601 atgggtcact ggatcaaaat gagccttgcc atctggaaaa attttcccca tattggcctg
     6661 atggacaata tcaaaaatac gttctgggtc cacccccatc aagacaaaac tgccgtaggt
     6721 gaagtaaagc gtgtcaatca aggcatccac ttgccctatc aaatcttgct gagcaggtgt
     6781 cttcttggct actttacctg ctgccttatc aagggcctga tgaagttgcg atacagcttg
     6841 accaaaatct tcttcagaag gactggctgc tcgaacaaac tccaccaatt cttctatttt
     6901 aaaaccagcc ctatgggttg caccctctaa atcccaagct cgaggttctt cttgggttcg
     6961 ttcatccatc atgtggtgga aggtcttgac cttattgaaa tgatagtcac ggctgacaaa
     7021 gactttttct gaagacaaaa caccaaaatg ttccaaggcc ttgtggatcc catcttgttg
     7081 attgctagtg gtaatgtgct tggcagcttc cttgacactg ctactaccat ttcccatagc
     7141 gaccgacata ccgacgccag ccagcatttc aaggtcatta tctgagtcac caaaagccat
     7201 gacttggttg aggtcaaagc catattcttt cccaactcgg cgaatgcctt ctaatttaga
     7261 atttccctga ttgatgacat ccgatgcaaa aggattgcta cgtgtcaatt tcaagtcttc
     7321 aaaatcagct gccgccttct cagattcttc tggtgtcatc agcatcaaaa cttggtagat
     7381 aggctgattc atcaggtgaa gcaggtcctc ttccttttgg ggaacaacct tgctgaccat
     7441 gcgattaaaa gactgactca ccgtccgagt taaaacagag ggaacgaagc gactaattcg
     7501 ttgggaaaaa gaacccagac caaaggacat gattttagaa cccaacatgg catccttggt
     7561 ccctagagca atctccgtgc cctctttttt agcatagcta attagatggc gcaaatgtaa
     7621 cttggaaata gggctcgtga acaagactct gtctttacta aagatatact ggccattata
     7681 ggttaccgca aaatccagat ccaaatcgtc catcaattcc ttaacaaaaa aaggtcctcg
     7741 ccctgtcgct acgccaacta gtaccccttg ttctttgaca atcttaatcg cgtccttagt
     7801 ggatttcaaa acactcttgc gattgttgac caaggttcca tcgatatcaa aaaaaacagc
     7861 tttgacttcc atcctattcc aatctcccct tttgtgatac aatgattata ccacatttca
     7921 gaaagagtga gtaaatcatg cctaagaaaa tccttgtttt acatacgggt ggaactattt
     7981 ccatgcaggc cgatgcttct ggcgctgttg tgacgagttc agataatccc atgaaccatg
     8041 tgtccaaccc acttgaagga atccaagtcc acaccttgga cttttttaac cttccaagtc
     8101 cccatatcaa acccaaacat atgctggtcc tctaccagaa aattaaagag gaagcagata
     8161 actacgatgg agtggtgatc acacacggaa ccgatacttt agaggaaaca gcctatttcc
     8221 ttgataccat ggaagttccc catatgccta tcgttctaac aggagccatg cgtagctcca
     8281 atgagctcgg tagtgatggt gtttataatt acctaagtgc tttacgagtg gccagcgatg
     8341 acagggctgc tgacaaagga gttttggtcg ttatgaacga tgaaatccac gctgccaagt
     8401 atgtcaccaa aacacatacg actaatgtca gcaccttcca gactccaaca catggccccc
     8461 tcggtctcac catgaaacag gaaatcctct acttcaaaac agctgaacct cgtgttcgct
     8521 ttgaccttga tcacatacaa ggtttagtcc ctatcatctc ggcttatgct ggtatgacag
     8581 atgagctgat tgatatgctg gatttagaac acttggacgg tttgattatc caagccttcg
     8641 gagctggtaa tattcccaaa gaaacggctc aaaaattaga aagccttctg caaaaaggaa
     8701 ttccagtcgc tctggtatca cgatgcttta acggtattgc cgagcctgtt tatgcctacc
     8761 agggtggggg cgtacagttg caaaaagcag gcgttttctt tgttaaagaa ctcaacgccc
     8821 aaaaagctcg cttgaaactc ctcatcgccc tcaatgccgg actaacagga caggctttga
     8881 aagactatat ggaaggctaa tactcttcga aaatctctgc aaaccacgtc agcgtcgcct
     8941 taccgtatgt atggttactg acttcgtcag ttctatctac aacctcaaaa acatgttttg
     9001 agctgacttc gtcagttcta tctacaacct caaaacagtg tgttgcgcag cctttaacca
     9061 gccgcctagt ccgctctatg gtattcatta agtcaacatc tcttgtttaa gagcacaaaa
     9121 tcaggaaatc ttctcgattc cctgattttt tctatttacg ttttcgtgtt gagctacgtt
     9181 ctgtcaaacc atgaggtaag agaacttcac gttcttccaa ctcttcctta tgcataatct
     9241 tggtcaacat acgcatacta atggcaccaa ggtcataaag aggttgggca atcgttgtca
     9301 agtttggacg ggtaaagcgt gagatttgtg aatcatcact agtaataatt tcaaaatctt
     9361 ctggcacaga aacaccctta tcagccaaac cgttcaagac tcctgctgcc aactcatcac
     9421 ctgtcacaac tgctgcagtt gcatttgatg aaatcaaacg ctctgctaag gcgtaaccat
     9481 catcatagct atatttagat tcaaatacca aaccctcact ataagtgatt cctgcttttt
     9541 tcaaggtttc cttgtagcca actaaacgaa ccttaccatt gatgtcatcc actagcggac
     9601 cgctaacgaa agcaatacgc tcattttctt tagcaaggta actcactgca tcaattgttg
     9661 cttgcttata gtcaatattg acacttggca actggtgctc aacatcgaca gttcctgcga
     9721 gaacaatcgg agtacgtgaa cgcgaaaatt ctgagcgaat tttatctgtc aagtgataac
     9781 ccatatagat aatgccatct acctgctttg aaaagagggt attgacaaca gaaacttctt
     9841 tctcgttatc ttcatcgcta ttagctagga caatattgta cttgtacatt tctgcaatat
     9901 catcaatccc cttagccaaa ctcgaaaaat aaccattggt aatatttgga atcacgacac
     9961 cgacagtggt tgtcttttta cttgcaagac cacgcgcaac tgcatttgga cgataatcca
    10021 aacgatcaat tacctctagc acttttttac gggtattctc ttttacattt ttattgccat
    10081 tgaccacacg gctgaccgtc gccatggaaa cacctgcttc acgagcgaca tcataaatgg
    10141 ttactgtatc atctgcattc attccttttc ctgtcctttc tatctcacac attcttttac
    10201 aagtagaggt actgattgaa gctctatatc tacttacaaa agtgaagatg tgaaaatttc
    10261 gttttcatat ttctacttat tccattctat cactaattgt aaacactttc aagtgttttt
    10321 tgaagattga ttgaaaaaat ttcatagaaa acctaggttt agctccttgc taccacctta
    10381 gactaaacaa aaaggaggaa actaagccct cctaaagtta tagtaaaatg aaataagaac
    10441 aggataaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa gtagaggtgt
    10501 actattctag tttccatcta ctataggtat tgttccattc actaccgtca attttagcac
    10561 atagtcttca tgaaaatatt atatcatcat aaccaaccag attcttttgc gatattagct
    10621 gcctctgttc gattaccagc atccagtttt gaaagaatat tggtgacata gtttcgaata
    10681 gttccgtttg agaggtaaag ctgatctgcg atttcttggt tggataagcc ccgggcgatt
    10741 ccctttaaga cagcaatctc ttgttctgtc aacggattgg gacgggtcat caccacttcc
    10801 atcaattcag gcgaatactc cttgcgtcct tcgaggacgg tgtgcaaggt ttgcatgagg
    10861 tctgcaatgc ttcgttcctt taatacataa gcatccactc cagccttgac cgcacgttca
    10921 aaatacccag cacgcttgaa ggtcgtcacc acagccacct ttgtttcaag cctttctgat
    10981 cgtatccact ccaagacttc aagacctgtc ttaacaggca tttctacgtc aaggatggcg
    11041 atatctacag actccttttc taatagttgg attgcttctt gcccattctt ggcttgaagg
    11101 acagactcca catctggttg aagcgtgagc aattgacaca tggcatctcg caacatactt
    11161 tgatcttctg cgactaatac tttcatctta gtttctctcc ttataaggta gtcgaacttg
    11221 cacttccgtt ggatgtttct gactgattac acttacttct cctgaaaatg gaaaaacacg
    11281 atctcggact gtatggagct catctccttt tagaaaagca aagccgcagc catcatcact
    11341 tactgttaaa atgagttcct tctctgtccg ttctaatttt aagtaagctt tagacgcttt
    11401 ggcatgtttg atgatattgg tcaccaactc aagcaaaatc atagaagcca ttgaatccaa
    11461 ttcttgagta aggctagcag tatctagttg gttatctgtt tccacctcaa ttccagcaat
    11521 ttctaacatc tttttcacag tctctagttc ggatgtcaaa gttctagact taagattttc
    11581 cacaatggtt cgaacttcac acattgattc tttgctaatt tgctgtattt ctcttaattc
    11641 cttttccact tgtggataag cctgcatttg aaatagctgc aaggctagat ctgtcttgac
    11701 actaagcata gcaaaggtat gtcccagact atcatgcaaa tcctgaccga tacgattgcg
    11761 ctcattttct gctagcaata gatttatctg ggtattttgc ttgacctgag cttctttcaa
    11821 atcctcgaca aggcgaattc gaaccgagcc caaagtcatc aaatcgacaa aagtaagaat
    11881 tacaagcata tagactagaa actcaacttc gattctctga aaaatcaaaa gtcgccccac
    11941 aacaaggact tgagcaagga gaaatgtcca tacatgtaaa gactttaaac tacgtacgcc
    12001 aaaatgataa attaagagat tggaaaggaa aaagaaaaac cagatataat tcccagcaac
    12061 aaaggcggta ttcccagcta catagaccag catgagcccc cagaaaagcc aagataggcg
    12121 ctggttctta gttattaaaa cacccaaata cgtcactaca aatagaatat caaccaataa
    12181 atgccaagca ggaagctccc caatcactac aggtaggatg ggaaaaagca taaaaatcaa
    12241 actggtccaa aacatatagt gtatgctttt cagtctttca agcattaagc attctcctta
    12301 tgaccttgaa ggtaaatagt caaaccaaat aaaactgctg aaaaaacaag taaataaact
    12361 gtggctgata gattgatgtc accctcgttt aagaaggtct tgagcaactc catcaattga
    12421 taggtcggga gacacttacc aactgcttgc atccagtctg gaaataaaga aatgggcatc
    12481 cagagtccgc ctaaaacagc caatcctaga taaagaagat tgcccacaac agacatcagc
    12541 tgactagttg gtaagagagt caaggtcaag ccaagtgcta caaaagcaat actccccaca
    12601 atcagcaaaa tcgcagctcc aacccatttt actagaggca tgtctacacc tcttacaaaa
    12661 tgtccaactg taaaaacaac cagaattgag accaaataat caaccagcat acttgttatc
    12721 tttgatagat aatattctac catatttaca gggctatgac gcaatgtttt ctgccagttg
    12781 ttcatcttat cggtatgtaa aacagctgga aatgagagga tagttgtcga catcatggaa
    12841 aaggcagtca tggagatgag gtaatcacgc ataaaattcg cgagttcacc tggtgtgtcc
    12901 tgatagatac cagaaaaaaa taaatagaaa gccgtcggca tccctaccga caatagataa
    12961 tagattaatt gtcgtttggt caataaaaat tctatcttac taagtgctat ccatcgtttc
    13021 atcttagtta tctcccttct gcgtctcttc aaagattgta ttcaacaaac tacgattatt
    13081 aacttcgatt tcttgcatcc tacatcctgc ttgggctaat agttcccaga aagcatctgc
    13141 ttctcgagtg actacttgta aagaatcttg ctttagggtc cagttttcaa ccaaatttga
    13201 ctgctctacg acttccttgt aagctatagg aaggataaag tgcttttcta tttcctcgct
    13261 acgcatagct agaggcgtcg tatctcgaat caactctccc ttatttaaga gcaaaatgcg
    13321 gtctgcagtg tgttccactt cctcaatata atgggacgaa tagagaatgg taactccctg
    13381 cgcttttaga tcctgaacaa tttcccaaaa acgttggcgg gttgaggtat ccatggccgc
    13441 agttggctca tctaaaaaga caagttttgg tcggccaatc aaggtcaaga caaaagagaa
    13501 aagacgcttt tgcccacctg acaatttttc tgccaattgc tctttctgtt gcttgtcaaa
    13561 ctgcaacagt tgatcgattt cctggttgct caagggattt ggataaatgc tttgaaagaa
    13621 agcaaccaac tctttgacct ttaatttctg aacgatgaca ttttcttgag gcagataacc
    13681 tctaatatag tctaactgag aactcgtcac tgacaagcct tggatggata cttgaccgct
    13741 tgtgaccagt ttatctccaa gcagacagtc caagagtgtg gtcttcccag caccatttgg
    13801 cccaatcaag gcgacgcatt caccttcagc tacctcaaag gaaatatcct tcaaaatagc
    13861 ctttcccttg atgtttttat gtaatccttg aaccttaatc atgtccatga tattctcctt
    13921 tcaaccactc cattcccata ggaaaggcga taaaaatcat aaatcctagt ccccaggcac
    13981 cacggatgaa ttggcgaagc aaggcttgat caaaccaacc tgtaaacatt tctaccaacc
    14041 ataccactag tgatagtcca ataaaaagat agagaaacgc ttttttcatt tttcaaactc
    14101 ctttttcaca tctgagacta atttcaaacc ttctcggata agccaggaca tcattccaaa
    14161 tcctgcaaat agctcccaag gaaaatgata gaaatcttca tccaatcctg agaacatgag
    14221 ataagtcatg actcctgctg ctactaaact cattgcgaca atcattttat ttttcatctc
    14281 ttcttcctcc gtttcatact tcaattatag acttttgaag ttggcggagc tagatgcttc
    14341 tgtcactaga aaatatgaca aatgtcataa aaattctgta caaaacaagc aagatacact
    14401 atataataaa acacaagtag aaaaaatcta aggcagcttt caaaagagat atcaaaccca
    14461 attcacacca taatgtaaac taatacttat ttaaaatcaa aaagagcgga aatttttatc
    14521 gcacaaacac atatattata agcagagcga aaaactcttc aattactttc ttaaaagttt
    14581 gtaagaatta tacaatctgt taaattttaa tttatgtacc taaactacag tattcattga
    14641 acaaattagg aaacaaaaaa gtatacgctg ctaaaatgaa ttactgaaat tcaaaaaaga
    14701 gagaagcaaa ctgattctct cttaatgtat ataatatcta gttaaaacaa aaagatacgc
    14761 ttacgtatct ctgaaa
//
LOCUS       DAVLXF010000043        14131 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLXF010000043 DAVLXF010000000
VERSION     DAVLXF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.043"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            82..879
                     /locus_tag="VYO12_001276"
     CDS             82..879
                     /locus_tag="VYO12_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1587884.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1027..2286
                     /locus_tag="VYO12_001277"
                     /pseudo
     CDS             1027..2286
                     /locus_tag="VYO12_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2382..3656
                     /locus_tag="VYO12_001278"
     CDS             2382..3656
                     /locus_tag="VYO12_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1587885.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3666..4544
                     /gene="truB"
                     /locus_tag="VYO12_001279"
     CDS             3666..4544
                     /gene="truB"
                     /locus_tag="VYO12_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1587886.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4785..5087
                     /locus_tag="VYO12_001280"
     CDS             4785..5087
                     /locus_tag="VYO12_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1587887.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKHQS
                     VMADLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5195..5833
                     /gene="udk"
                     /locus_tag="VYO12_001281"
     CDS             5195..5833
                     /gene="udk"
                     /locus_tag="VYO12_001281"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1587888.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5961..7301
                     /locus_tag="VYO12_001282"
     CDS             5961..7301
                     /locus_tag="VYO12_001282"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1587889.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7279..7491
                     /locus_tag="VYO12_001283"
     CDS             7279..7491
                     /locus_tag="VYO12_001283"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1587890.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7488..8363
                     /locus_tag="VYO12_001284"
     CDS             7488..8363
                     /locus_tag="VYO12_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1587891.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQVPFE
                     TESIVSVVESLRING"
     gene            8356..9171
                     /locus_tag="VYO12_001285"
     CDS             8356..9171
                     /locus_tag="VYO12_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1587892.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9164..9595
                     /locus_tag="VYO12_001286"
     CDS             9164..9595
                     /locus_tag="VYO12_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1587893.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9602..11269
                     /gene="recN"
                     /locus_tag="VYO12_001287"
     CDS             9602..11269
                     /gene="recN"
                     /locus_tag="VYO12_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1587894.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDMTEAALTQARELLRNREK"
     gene            11271..11999
                     /locus_tag="VYO12_001288"
     CDS             11271..11999
                     /locus_tag="VYO12_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1587895.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12078..13901
                     /gene="lepA"
                     /locus_tag="VYO12_001289"
     CDS             12078..13901
                     /gene="lepA"
                     /locus_tag="VYO12_001289"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1587896.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13982..>14131)
                     /locus_tag="VYO12_001290"
     CDS             complement(13982..>14131)
                     /locus_tag="VYO12_001290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587897.1"
                     /translation="HSFEVVDKTDEVSSKHGFEVVDETDEVSNHTYGKATMTWFEEIF
                     EEYKN"
BASE COUNT         4177 a         2577 c         3358 g         4019 t
ORIGIN      
        1 cttttatgaa atacagaaaa cgcttcctaa aacctaaagt ttgtgatata attatcaaga
       61 aaaaagattt aggagtttat tatggtttct tcagaattta tctcaaagat tgaatttgct
      121 tgcaataaga aagaaagtct ttatagtcaa agcaaattta agtatgcgat tcgttcgatg
      181 ttcgcaggtg catttttaac cttcagtact gctgcaggtg cagttggggc tgacttgatt
      241 aataaaattg caccaggtag tggacgcttc ctctttccat tcgtttttgc ttggggcttg
      301 gcctacattg tttttttgaa tgccgagttg gtcacttcaa acatgatgtt cttgactgct
      361 ggtagtttct taaaaaaaat ctcttggaga aaaacagctg agattttact atactgtacc
      421 ttgttcaacc ttatcggagc cttgatagca gggtggggct ttgctcattc ggcagcctat
      481 gcgaatctga tacacgatag tttcatctca ggtgttgttg agatgaagtt aggccgctca
      541 aatgaattgg tcttgcttga ggcgattttg gcaaatattt tcgtaaatat tgcgattctg
      601 tcatttattt tggtcaaaga tggtggtgcc aaactttggc ttgtgttgtc agctatttac
      661 atgtttgtat tcttaacaaa cgagcacatt gcggcgaact ttgcttcttt cgcgattgtg
      721 aaattcagtg ttgctgcgga ttcaattgcc aacttcggtg ttggaaatat gcttcgccac
      781 tggggtgtga ctttcatcgg aaactttatc ggaggaggcc tcttgatggg tcttccatat
      841 gccttcctca ataaaaacga agatacttat gtagattaag aaaatgagca cgattgagtc
      901 gtgctttttt cattttcaaa ataaggtaat agctatttct tatatcaaaa tatagaaaac
      961 tgatatttgt aaactataac tcaaggtgct acaatatcct taataaaatg atatggaggt
     1021 caccttatga cttgtgattt taaatctgaa actctacaac tacatgctgg tcaagttgtg
     1081 gctccagcta ctaagtctcg tgcagtgccg atttatcaaa caacattttt tgtttttgat
     1141 gacacgtagg aaggtgccga tctgtttgcc ttgaggaaac cagggaacat ttatactcgt
     1201 atcaccaatc ctacaacagc tgcccttgaa ggtggtgttg aagcgctagc aacagcatca
     1261 ggtatgactg cagtgactta tacgattttg gcgcttgccc atgctggtga ccatgtagtg
     1321 gctgcttcga ctatttacgg tggaaccttc aatcttttga aagaacccct tcctcgttat
     1381 ggtatcacaa caacctttgt cgatgttgat aatttggagg aagtagaagc agctatcaaa
     1441 gacaatacca aacttgtctt gattgaaacc ttgggtaact ccttgattaa tattccagac
     1501 ctggaaaaac tggcagagat tgctcataaa catcaaatcc cacttgtgtc agacaatact
     1561 tttgcaatac cttatttgat taacgtcttc tctcatggcg ttgacattgc cattcactct
     1621 gcgactaagt ttatcggtgg gcatggtaca actattggag gaataattgt cgatagtggt
     1681 cgttttgact ggatggcttc agggaaattc cctcaatttg ttgacgaggg ttcaagctgc
     1741 cacaatttga gctatactcg tgatgtgggt gcagtagcct ttattatagt tgttcgagtt
     1801 caattgcttc gtgatacagg tgcagccttg tcaccattca atgctttcct cttgctacaa
     1861 agacttgaaa ccctttcact tcgtgtggaa cgccatgtac aaaatgctga gacaattgtt
     1921 gattttcttg tcaaccatcc taaggtagaa aaggtaaatt atccaaaact tgcagatagt
     1981 ccttattatg ccttggctga gaaatacttg ccaaaaggtg tcggttcaat ctttaccttc
     2041 cacgtcaaag gtgacgagga agaagcacgc aaggtcattg atcatttaga aatcttttct
     2101 gaccttgcaa acgcggcaga tgctaaatcg cttgttgtcc atccagcaac aatcactcac
     2161 ggtcaattgt cagaaaaaga cctagaagca gcaggtgtca caccaaatca aatccacttg
     2221 tctatcggtc ttgaaaatgt agaagatttg attgaagact tacgcttggc cttggaaaaa
     2281 atttaaagta aaagaagata aacagtgggt tttgactcac tgtttttgat tttccttcat
     2341 gcatgatata atggttacag aagtctagaa agaggaacga tatgaacgaa atcaaatgtc
     2401 ccaactgtgg ggaagtcttt acagtaaatg agagtcagta tgccgaactc ttgtcccaag
     2461 tgagaacggc agagtttgat aaggaactac acgataggat gaagcaggaa ctggccttgg
     2521 cggagcaaaa ggccatgaat gagcaacaga ctaaactggc tcagaaggat caagaaattg
     2581 cgcaattaca gagtcagatc caaaactttg atacagaaaa agaattggcc aagaaagagg
     2641 ttgaacagac aagccatgag gctctcttgg ctaaggacaa ggaagtacag ctcttagaaa
     2701 atcagttggc taccttgcgt ttggagcatg aaaatcaact acaaaagacc ctttctgacc
     2761 tagaaaaaga acgggatcag gttaaaaacc aactactttt gcaggaaaag gaaaatgaat
     2821 tatctttggc ttctgttaag caaaactacg aagcccagct caaggcagct agtgaacaag
     2881 tcgagtttta taagaatttt aaggctcaac aatctacaaa agcgattggg gaaagcctag
     2941 aacagtatgc agagagtgag tttaacaagg ttcgtagttt cgcctttcca aatgcttact
     3001 ttgagaagga taacaaggtc tcttcgcgtg ggtctaaagg ggactttatc ttccgtgagt
     3061 gtgatgaaaa tggagttgaa atcatttcta tcatgtttga gatgaaaaac gaagcagacg
     3121 gaacagagaa gaagcacaag aatgcagatt tttacaagga attggacaag gaccgtcggg
     3181 agaagaactg tgagtatgcc gttttggtga ccatgcttga ggctgataat gactacttta
     3241 acacagggat tgttgacgtc agtcacgagt atgaaaaaat gtatgttgtt cgtcctcaat
     3301 tctttatcca attgattggt ctcttacgta atgcggcgct aaattcccta aaatacaagc
     3361 aggagttggc cttggttcgc gagcaaaata ttgacattac gcattttgag gaagatttgg
     3421 atgcctttaa gctagctttt gctaagaact ataattcagc ttcgactaac tttggaaaag
     3481 ctattgatga aatcgacaag gccatcaaac gcatggaaga ggttaagaaa ttcctgacta
     3541 catctgaaaa ccaactccgt ctagctaaca acaaattgga agatgtctct gttaaaaaat
     3601 tgacccggaa aaatccaaca atgaaagcga agttcgaagc actgaagggg gagtagaaag
     3661 caaaaatgaa cggtattatc aacttaaaaa aagaagcagg aatgacctcg catgatgcgg
     3721 tttttaaact gcgtaagatt ttgggaacca agaaaattgg tcatggtgga accttggatc
     3781 cggatgtggt gggtgttttg ccgattgcgg ttggcaaggc gacacgcatg gtcgagttta
     3841 tgcaggacga gggtaagatc tatgaggggg aaattactct gggttattcc acgacgactg
     3901 aggatgctag tggggaagtg atcgcagaaa cccctgtttt gtctcccttg gatgaaaagc
     3961 ttgttgatga agcgattgct agcttgactg ggcctattac ccagattccc cctatgtatt
     4021 cggcagttaa ggttaatggt cgcaagctct atgagtatgc gcgtgctggt caggaagtgg
     4081 agcgtccaga acgtcaggtg accatttatc aatttgagcg aacaagtccg atttcttatg
     4141 atggccaact tgctcgattc acttttcgtg taaaatgcag taaagggact tacatccgta
     4201 ctttgtcagt tgatttgggt gaaaagcttg gttatgcggc tcatatgtcc catttgactc
     4261 gtactagtgc tgctggctta caattagaag acgctcttgc cttggaggaa attgctgaaa
     4321 aagtagaggc tgggcaatta gattttctcc atcctttaga gattgggaca ggtgaccttg
     4381 tcaaagtttt cctaactcca gaagaggcta cagaagttcg ctttggtcgt tttattgagc
     4441 tagaccaaac ggacaaagaa ctggctgcct ttgaagatga taaattgtta gccattctag
     4501 aaaaacgggg caatctctat aagccaagga aggtttttag ctagatcgtt taggaataaa
     4561 aatcgggtga tagataacaa ttgcttgata aaaccccata ctaatagtag aatggttttg
     4621 ggaattataa tattccaatt gttgcgagtt gtaggtactc aaataatcta tatagaaatt
     4681 tagaggtgtg aaatgaagca atttaaaatt ctttcagata aatatttaga gtccattaca
     4741 ggttctggtg ggaacttagg cccaggattt ggtgtgataa ttccatgatg cgaaatgagt
     4801 ttcgagaaag ggtggagcaa cttcttcaac aaaaagaaat aaatgaaaat agtgagttga
     4861 gtcacctgtt tcgtcttgct atacaaaatt tagacagaaa tgaaaaacac caatcggtca
     4921 tggccgattt gagtcaaggg ttgtcacttt acctcatgac gcatcattac caggcaccta
     4981 agtctgtcat tgattttggt ttatggattg ccaaagctcc tagccaggaa agagggagac
     5041 tggctttctt gcagatgctt gctcaaaccc tacagggctt tcgttaaaac tgtcgaacat
     5101 cagagggaag actctggtgt tttttgctga tgagactata aattttgtaa aaaatgtgat
     5161 agaatagacg acggataaaa taacggagga tagcatgcaa aatagaccaa tcattattgg
     5221 agtgacaggt ggttctggtg gtggtaaaac cagtgtttca agagccattt tatcgcattt
     5281 tcctgatgaa aagatttcca tgattgagca tgattcatac tacaaggatc agtctcattt
     5341 gacctttgaa gagcgtgtca aaaccaacta tgaccatcct tttgcctttg atacagactt
     5401 gatgatcgag cagattaagg aattgttggc agggcgtccg gtggacatcc cgacctacga
     5461 ctatacagag catacacgga gtagcaagac ctatcgtcag gagcctcaag atgtctttat
     5521 cgttgagggc attttggtct tggaggacaa gcgtctgcgc gatttgatgg atatcaagat
     5581 ttttgtggat acggatgacg atgtgcgtat tattcgtcgt atcaagcgtg atatggagga
     5641 acgtggccgt agccttgata gcgttattaa ccagtactta ggtgtggtca aaccaatgta
     5701 ccaccagttt atcgagtcaa ctaagcgtta tgctgatatc gtcattcctg aaggggttag
     5761 caataccgtg gctatcgacc tgttgacgac caagattgca aagattttgg aagaagctcg
     5821 aaacagcaaa taatcacatg tggaggcctt gcctcctttt tctatttttc cctcataatg
     5881 gtacataaat ggagattttt aggcatattt ttggtataat aatacccata aaagagcaag
     5941 taaatgaata gtaggtggag atggaaaagt atttatcggt aacaactttg accaaatatc
     6001 tgaaaatgaa attcgataaa gacccatact tggaacgggt ctatttaact ggtcaagttt
     6061 ccaactttcg taaacgacct actcaccaat atttctccct aaaggatgac catgcagtta
     6121 ttcaagcgac catctggtct gggatttatc agaaattagg gtttgacctg gaagaaggaa
     6181 tgaagatcaa tgtgattggg cgtgtacagg tctatgaacc aagtggtagc tactccatca
     6241 tcattgaaaa agctgagcct gatggggttg gggcgcttgc gattcagttt gaacaactta
     6301 agaaaaaatt gacagaagaa ggcctgtttc aagaacgctt caagcaagct ctaccccaat
     6361 tttctaagag aattggggta gtaaccagcc gtagtggagc cgttattcga gatattatca
     6421 cgaccgtcag caggcgattc ccaggtgttg acatccttct ttatcctacc aaggttcaag
     6481 gtgaaggggc tgcggaggaa attgctagaa atattgcgcg tgctaatcaa cgggacgatt
     6541 tggatttgct cattattggt cgtggaggtg gttctattga ggatctctgg gcctttaacg
     6601 aagaaattgt ggtacgagct atttttgaat ctcgtttgcc agttatctct agtgtggggc
     6661 atgagacgga tgtgaccttg gcagattttg tggcagatcg acgcgctgca acgccaacag
     6721 cggcggctga actggcaaca cctgtgacca agttggatgt attaactcat ttgcaaaatc
     6781 aggaaaaacg gatggcaacg gcagtccgaa atgttctatc taagaaacaa gaggctttga
     6841 aaaaatgcag tcagtctgtt atctttagac aacctgagcg cttgtatgac ggttatttgc
     6901 aacgcttgga ccaactgcaa ctgcgtttga aacaaagttt gcgaactcgg atttctgata
     6961 acaaacaatt agttcaagca agaactcatc aattagtaca attatcacct gttaccaaaa
     7021 tccaacgcta tcaagaccgt ttaggacagt tggacaagct cttaggtagc caaatggcgt
     7081 tagtttatga cgccaaggtt gctgaggcca agcgactttc ggaagctttg ctcatgttgg
     7141 atactagccg aatcgtggcg cgtggttatg ctattgtcaa aaaagaagag tccgttgtag
     7201 attcggttga gagtttgaag aaaaaagatc aagtaacgct tttgatgcga gatggtcaag
     7261 tagaattaga ggttaaagat gtcaaaacaa aagaaatttg aggaaaatct agcagaactg
     7321 gaaaccattg tccaaagttt ggaaaatggt gaaattgctc tggaagatgc gattactgcc
     7381 tttcaaaagg gcatggtctt gtcaaaagag ctccaagcta cgctggacaa ggctgaaaag
     7441 accttggtca aggtcatgca agaagacgga acagaaagtg attttgaatg aaaaagcaag
     7501 aaaaattagc tcttgtcgag tcggctttgg aagattttta tggagaccag cagtttgcct
     7561 ctagtttacg ggagtctgtt ctctattcta ttcatgctgg tggcaagcgt attcggcctt
     7621 ttctcttgtt agaagttctg gaagccttgc aggttgccat caaacctgct cacgcgcagg
     7681 tagctactgc cttggagatg attcatacag ggagcttgat tcacgatgac cttcctgcta
     7741 tggatgatga cgattatcga agagggcggt taaccaatca caagaaattc ggtgaagcta
     7801 tggccatttt ggctggagat gccttattct tagacccata tgccttgatt gcgcaggcag
     7861 atttgccaag tcagattaag gtggacttga ttgccaactt atcccttgct tcaggtagtc
     7921 tgggtatggt ggcagggcaa gttttggata tggagggcga acaccagcac ttgtctctgg
     7981 aagaacttca gactattcat gccaataaga ctgggaagtt actagcctat cccttccaag
     8041 cggcagctat tatagctgaa ttgtcacctg aaatgcaggt gaagctgaaa actgtgggtg
     8101 aattgattgg acttgctttt caagtcagag atgatgtact ggatgtgaca gctagttttg
     8161 aggaaatcgg taagacacct caaaaggatc tgcaggcaga aaaatcaacc tatcctgcct
     8221 tgttgggctt ggaagagtcc attgcctttt gtaaccaaac cctggatcaa gctaatgaaa
     8281 aattggaaga aattgcccag caggttccct ttgaaacaga atcgattgta agtgtagtag
     8341 aaagtttgag aatcaatggc taaggaaaga gtggatgtac tagcttataa acaggggttg
     8401 tttgaaacga gagagcaggc caagcgaggt gtgatggctg gcctagtcgt agcagtcctt
     8461 aatggagaac ggtttgacaa gccaggagag aaaattccag atgataccga attaaaactc
     8521 aagggggaga aactcaagta tgtcagccgt ggtggtttga aactggaaaa ggccttgcag
     8581 gtctttgatt tgtcggtgga tggcgcgact acgattgata tcggggcctc tactggaggt
     8641 tttaccgatg tcatgctaca gaatagtgcc aagttggtct ttgcagtcga tgttggtacc
     8701 aatcagttgg cttggaaatt acgccaagac ccacgagttg tcagcatgga gcagttcaat
     8761 ttccgctatg ctgaaaagac tgatttcgag caggagccga gctttgccag tattgatgtg
     8821 agtttcattt cccttagtct gattttgcca gccttgcacc gtgtcttggc tgatcaaggt
     8881 caggtggtag cacttgtcaa acctcagttt gaggcaggac gtgagcagat tgggaaaaat
     8941 ggaattattc gagatgctaa gattcatcag aatgtccttg aatctgtaac agctatggca
     9001 gtagaggcag gtttttcagt ccttggcttg gacttttctc ccatccaagg tggacatgga
     9061 aatattgaat ttttagcgta tttgaaaaaa gaaaagtcag caagcaatca gattcttgct
     9121 gagattaaag aagcagtaga gagggcgcat agtcaattta aaaatgaata aaaaagaaag
     9181 acttgaaaaa attagaagat tggtgacaga ttatcaaatc ggcacgcaag aagaaattgt
     9241 agaacatttg aaagaagcag gtatcactgc cactcaggcg acggtatccc gagatatcaa
     9301 agagttaggt attgtcaaaa ttcctttgag agacaacacc tatgtctacg aattaccaaa
     9361 atcaatcgta aaaagtctgc aactggctga agacaatatc gaatcggctg aattgatgga
     9421 taagatgatc aatctccaag ttattccagg aaatacggct tttgtaaaag ctcagttaat
     9481 cgatactttt gcagacaaga tttttagttg tttgactgat gatagctcga ttttagtcat
     9541 tgccagaagt ggaagtctag cagaggaaat ctttgaacaa gtaaaaaatt ggtaggtcta
     9601 tatgttactt gaaatttcga taaaaaactt tgccattatt gaggctattt ccctcaattt
     9661 tgaaaagggg atgactgtcc tgactggtga aacgggtgca gggaagtcga ttatcattga
     9721 tgccatgaat atgatgttgg gagctcgtgc gacgacagat gttattcgtc atggtgcacc
     9781 aaaggcagag attgaggggc ttttctcagt tgagaatagt cgccttttac aggaaatttt
     9841 tgatgagcaa ggtttagaat taggtgatga aattatcatc cgtcgggaaa tcttgcaaaa
     9901 tggtcgtagt atcagccgtg taaatggtca gatggttaat ctgtctgttt tgcgagctat
     9961 tgggcaacat cttgtagata ttcatggtca gcatgaccat gaggagttaa tgcgtcccca
    10021 actgcatatc cagatgttgg atgaatttgg tgatgccgct ttttgggact tgaaagaaac
    10081 ctatcaaacg agttttgatg cctatcggaa aatgcgcaag caggttctgg aagtcaagaa
    10141 aaaccaacag gaacacaagg cacgtatcga aatgttggaa tttcaaatgg cagagattga
    10201 ggcagcaaac ttgcaggctg gagaagactt ggctctcaat caagagcgag ataaactcct
    10261 caaccacaaa aatattgcgg atacactgac caatgcctac agtatgttgg acaatgaaga
    10321 tttttcaagt ctggccaatg ttcgttcagc tatgaatgac atggaaagtg tcgaagagta
    10381 tgatcctgaa taccgtgaaa tttcaagctc tctgtctgag acctactatg ttttagaaga
    10441 cattagcaaa cgtttggaag ctatcattga ggaccttgat tttgatggca atcgcctgat
    10501 gcaggttgag aatcgtttgg acctccttca tactattact cgtaagtatg gtgggactgt
    10561 tgatgatgtt ttgctttatt ttgctaagat tacggaagaa tacaatctct tgacaggcaa
    10621 taatctttcg tctgaggaca tggaagcaga gcttaagaag ttggaagtca atcttgtcaa
    10681 tttggcaggt caacttgctt ctgctcgtca taatttggct cagcaactcg aagctgagat
    10741 taaacaagaa ctgcaagatc tttatatgga aaaagcccag tttcaggttc gttttagtaa
    10801 gggaaaattc agtcgtgagg gaaatgaaat ggttgagttt tatatttcaa ccaaccctgg
    10861 agaagacttt aaacccttgg ttaaggttgc ttctggaggg gaattatctc gtctcatgtt
    10921 agccattaag tctgcctttt cacgtaaaga aggcaagact agcattgtct ttgatgaggt
    10981 ggatacggga gtttcaggtc gtgttgctca agctattgct cagaaaattc ataaaattgg
    11041 tcagtatggt caggttttgg ctatctccca tttgccacaa gtaattgcga ttgcagatta
    11101 tcaatttttt attgagaaga ttagtaatga ccattcaacg gtttcgactg ttcgtctctt
    11161 gacagtcgaa gagcgagtgg aggaagttgc caagatgttg gcaggtgatg atatgacaga
    11221 agcagccctg acgcaagcca gagaattgtt gagaaacagg gagaaataag atgacagatt
    11281 attatgtaat tggagatgtt cacggaaaag ctgggatgct ggaagacctt ctcaaaacct
    11341 gggatggtca gacccagttg ctctttctag gtgatttgat tgacagaggt gaggatagtc
    11401 accgtgttct tgaaatggtc aaggacttgg tggacaatca aggggctatc tgtttgtctg
    11461 gaaaccacga gtatatgttt ttgacttggc tagatgaccc agaagaaagt tatgaccatt
    11521 atcgtcgcaa tggtggagat acaaccatta actctatcct aggtcgtccc ttggatgcac
    11581 cagttgatgg agtagaagat gccaagcggg ttgccgctga agcggcagac ttggtcgaat
    11641 tcattcgtca aatgccattt gtagtagaga cagacaagta tatctttgtt cacgcaggta
    11701 ttgatttgac cttggacgac tggcatgaaa caacagatta taaaaaagta tggcttagaa
    11761 aaccattcca cggagctgaa aatcatactg gaaaaaccat tgtcttcggt catacaccgg
    11821 tctatggttt gttacagcaa gaacgcggta catctgagct ttggactaca gaagatggca
    11881 agattggaat ggatggagga gctgtttatg gtggtgtcct tcacgggatt gtctttacag
    11941 accaaggaat gacagaacac cactttatcg aaaatgacgg ctttgtcgct gaagattagt
    12001 actcctagca gggtatggtc ttgtcaaaat atcaaaaaca atttataata aatagatacc
    12061 ctgaaaggaa gagaatcatg aacttagaag aattgaaaaa acgacaggag aagatccgta
    12121 acttctctat tatcgcccat attgaccacg gaaaatcaac tctagcagac cgcattttgg
    12181 aaaaaacaga gacggtttca agtcgtgaaa tgcaggccca gcttttggat agcatggatc
    12241 tagagcggga acgtggaatt actattaagt tgaatgccat cgagttgaat tacactgcaa
    12301 aagatggaga aacttatatt ttccacttga ttgacacacc agggcacgtt gactttacct
    12361 atgaagtttc acgttcgcta gctgcctgtg agggtgctat tttggtggtc gatgcggctc
    12421 aaggaattga ggctcaaact cttgccaacg tttatctggc tttggataat gatttggaaa
    12481 tcatgcctat cattaataaa attgacctgc cggctgcaga tccggagcgc gtgcgtacag
    12541 agattgaaga tgtgattggt ttggatgcca gtgaagcagt tttggcttct gccaaggctg
    12601 ggattgggat tgaagaaatc ctcgagcaaa ttgtagaaaa agtaccagca ccaacgggtg
    12661 atgtgacggc gccacttaag gccttgattt tcgactctgt ttacgatgct taccgtgggg
    12721 ttatcctcca agtgcgtgtc atggacggag tggtcaaacc tggtgataag attcagctca
    12781 tgagcaatag taagaccttt gatgtggccg aagtcggtat ttttacacca aaagcggttg
    12841 gtcgtgattt ccttgcgact ggtgatgttg gttacattgc ggcgtctatc aagacggttc
    12901 aggatactcg tgtgggtgat accgttacct tggcaaccaa tcctgcggca gaaccattac
    12961 atggttataa gcagatgaat cctatggtct ttgcgggtct ctacccaatc gaatcaaaca
    13021 agtacaatga cctgcgtgaa gcgcttgaaa aattgcaact gaatgatgct agtcttcagt
    13081 ttgaaccaga aacatctcag gcacttggat ttggttttcg ttgtggattt cttggacttc
    13141 tccatatgga tgttatccag gaacgtttag agcgtgagtt caatattgac ctcatcatga
    13201 cagctccgtc tgttatttac aaagttaatt tgaccgacgg tgagtctatg gatgtgtcta
    13261 acccatctga gtttccagac ccaactaaga ttgcgaccat tgaagagcct tatgtcaagg
    13321 cgcaaatcat ggtaccacag gagttcgtcg gagcagtaat ggagctagct cagcgtaagc
    13381 gtggggactt tgtgactatg gactatattg atgataaccg tgtcaatgtt atctatcaaa
    13441 ttcctcttgc tgaaattgtc tttgacttct ttgataaact taagtcttca acacgtggtt
    13501 atgcaagctt tgactacgaa ttgtcagaat atcgcccatc taagctggtg aaaatggata
    13561 tccttctcaa tggagataag gtggatgctc tcagctttat cgttcacaag gactttgcct
    13621 acgaacgtgg gaaactcatc gttgataaac tcaagaaaat catccctcgc caacaatttg
    13681 aagttccaat ccaagcggct attggacaca agattgtcgc tcgtactgat atcaaggccc
    13741 ttcgtaagaa cgtacttgct aaatgttatg gtggtgacgt ttctcgtaag cgtaaactcc
    13801 ttgaaaaaca aaaagctggt aagaaacgca tgaaatccat cggatcagtt gaagttccgc
    13861 aagaagcctt cctcagcgtc ttgagtatgg atgaagaata agttatcaga aatgcctttc
    13921 cttgttcaca ggaaatttat ataagaatca atagtaaagc aggcttagat ggtctgcttt
    13981 tttagttttt atactcttcg aaaatctctt caaaccacgt catcgtcgcc ttaccgtatg
    14041 tatggttact gacttcgtca gtttcatcta caacctcaaa accatgtttt gagctgactt
    14101 cgtcagtctt atctacaacc tcaaaactgt g
//
LOCUS       DAVLXF010000044        14129 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLXF010000044 DAVLXF010000000
VERSION     DAVLXF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.044"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            167..781
                     /locus_tag="VYO12_001291"
     CDS             167..781
                     /locus_tag="VYO12_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1587898.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGNAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFALILSSVVTGKVG
                     SLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAVLRLVF"
     gene            885..1715
                     /locus_tag="VYO12_001292"
     CDS             885..1715
                     /locus_tag="VYO12_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1587899.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKIFDKITVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            1756..1833
                     /locus_tag="VYO12_001293"
                     /pseudo
     CDS             1756..1833
                     /locus_tag="VYO12_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            1937..2767
                     /locus_tag="VYO12_001294"
     CDS             1937..2767
                     /locus_tag="VYO12_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1587900.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2921..3775
                     /locus_tag="VYO12_001295"
     CDS             2921..3775
                     /locus_tag="VYO12_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1587901.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
     gene            3875..5248
                     /locus_tag="VYO12_001296"
     CDS             3875..5248
                     /locus_tag="VYO12_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1587902.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAYFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQRHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEDRMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            5241..6302
                     /locus_tag="VYO12_001297"
     CDS             5241..6302
                     /locus_tag="VYO12_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1587903.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEESSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            6304..6996
                     /locus_tag="VYO12_001298"
     CDS             6304..6996
                     /locus_tag="VYO12_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587904.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIVIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(7026..7595)
                     /locus_tag="VYO12_001299"
     CDS             complement(7026..7595)
                     /locus_tag="VYO12_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1587905.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7730..8344
                     /locus_tag="VYO12_001300"
     CDS             7730..8344
                     /locus_tag="VYO12_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1587906.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFISSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            8346..9992
                     /locus_tag="VYO12_001301"
     CDS             8346..9992
                     /locus_tag="VYO12_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1587907.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKVSPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIINVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            10004..11290
                     /locus_tag="VYO12_001302"
     CDS             10004..11290
                     /locus_tag="VYO12_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1587908.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            11351..11884
                     /locus_tag="VYO12_001303"
     CDS             11351..11884
                     /locus_tag="VYO12_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1587909.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11962..12432
                     /gene="nrdI"
                     /locus_tag="VYO12_001304"
     CDS             11962..12432
                     /gene="nrdI"
                     /locus_tag="VYO12_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1587910.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12725..13987)
                     /locus_tag="VYO12_001305"
     CDS             complement(12725..13987)
                     /locus_tag="VYO12_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1587911.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIIIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         4222 a         2644 c         3209 g         4054 t
ORIGIN      
        1 gtgttttgag ctgacttcat cagttctatc tgcaacctca aaacagtgtt ttgagcaacc
       61 tgcggctagt ttcctagttt gctctttgat tttcattgag tattattaag acaatatgag
      121 gagaaagatg aaagaaaaag gattttagga gggggcgcag gcagccatgc caacggctct
      181 tggctatgtc agtatcggcc tggcctgtgg gactatcggt gcgccctatg tgacacctgt
      241 tgagatgggc ttgatgagtc tctttgttta tgctgggaat gcccagtttg ccatgttggc
      301 actgattgtg gttcaagctc ctgtggcagc tattgctatg acggtttttc taatcaactt
      361 gcgtctcttt ttgttgagtt tacacgcatc gacttatttc cgtcatacca gtctttggta
      421 caatatcggt atgtctagta tcttgacaga tgagacctat ggcgttttga tgggtgaatt
      481 ggcccataca gacaaggtaa atcctatgtg gatgcacgga aacaatctta acagctatgt
      541 ggcttggttt gtggggacag tagtcggaac ggctctgggt ggcctgctac caaatccaga
      601 aatctttgcc ttgattcttt caagcgtagt gacaggtaag gttgggagcc ttcctcaaat
      661 taaatggcta gacttcttag ccgtctttcc aacagcttgg gtagcctttc gctaccgcaa
      721 tctagtcgga acagttctct ttggagtagt cttgattgcc gtcttgcgtt tggtctttta
      781 aatcagccat aaagaaaacc tatcacagat atatatatca tataatggtg taaatgattt
      841 ttttctgtta agactataag gtattctatt ttggaggaaa tgacatgaaa aaaatcgtta
      901 aatactcatc tcttgctgcc ctagggcttg ttgctgcagg tgtgctagca gcttgctcag
      961 gtggtgctaa gaaagaagga caagcagcta gcaagaaaga aattattgtt gcaactaatt
     1021 cttcaccaaa accattcaac tatgaagaaa atggcgaatt gactggttac gagattgaag
     1081 tcgttcgcgc tatctttaaa gactctgaca agtatgatgt caagtttgag aagacagagt
     1141 ggtcaggtgt ctttgcgggt cttgacgctg accgttacca aatggctgtt aacaacatca
     1201 gctacactaa agaacgtgcg gagaaatacc tttatgcagc cccaactgtt aaagacccta
     1261 acgttcttgt agtcaagaaa gacgattcta gcatcaaatc gcttgatgat gttggtggaa
     1321 aatcaacaga agttgttcaa ggaacaacat ctgctaaaca gttagaagac tacaacaaac
     1381 aacactcaga taatccaact atccttaact atactaaagc aaacttacaa caaatcatgg
     1441 gccacttgag cgatggtcag tttgactaca agatttttga taaaatcact gttgaaacag
     1501 tcatcaagaa ccaaggtttg gacaacttga aagtcatcga acttccaagc gaccaacaac
     1561 cttacgttta cccacttctt gctaaaggtc aagatgagtt gaaaacattt gtagacaaac
     1621 gtatccaaga actctacaaa gacggaactc ttgaaaaatt gtctaaacaa ttcttcggag
     1681 acacttacct accggcagaa gctgatatta aataatttct tgaaatcatc cattctgagt
     1741 aagatgggtg atttttcagt tcgctaccgc aatctagtcg gaacagttct ctttggagtg
     1801 gtcttgattg ccatcttgcg tttggtcttt taaattaccc ataaaaaaaa cttatcacag
     1861 agatagatat catataatgg cgtaaatgct ctttttctgt taagattata aggtattcta
     1921 ttttggagga aatgacatga aaaaaatcgt taaatactca tctcttgctg ccctaggact
     1981 tgttgctgca ggtgtgcttg cggcttgctc agggggtgct aagaaagaag gagaagcagc
     2041 tagcaagaaa gaaatcatcg ttgcaaccaa tggatcacca aggccattta tctatgaaga
     2101 aaatggcgaa ttgactggtt acgagattga agtcgttcgc gctatcttta aagattctga
     2161 caaatatgat gtcaagtttg aaaagacaga atggtcaggt gtctttgctg gtcttgacgc
     2221 tgaccgttac aatatggctg tcaacaatct tagctacact aaagaacgtg cggagaaata
     2281 cctctatgcc gcaccaattg cccaaaatcc taatgtcctt gtcgtgaaga aagatgactc
     2341 tagtatcaag tctctcgatg atatcggtgg aaaatcgacg gaagtcgttc aagccactac
     2401 atcagctaag cagttagaag catacaatgc tgaacacacg gacaacccaa ctatccttaa
     2461 ctatactaag gcagacttcc aacaaatcat ggtacgtttg agcgatggac aatttgacta
     2521 taagattttt gataaaatcg gtgttgaaac agtgatcaag aaccaaggtt tggacaactt
     2581 gaaagttatc gaacttccaa gcgaccaaca accgtacgtt tacccacttc ttgctcaagg
     2641 tcaagatgag ttgaaatcat ttgtagacaa acgcatcaaa gaactttata aagatggaac
     2701 tcttgaaaaa ttgtctaaac aattcttcgg agacacttat ctaccggcag aagctgatat
     2761 taaataattt cttgaaatca tccattctga gtaaggtggg tggtttctca gtttttaggg
     2821 atatagtttt aaactatata tctgactatc taataacaat ttgtgaaatc aaacaaattg
     2881 tgagatacta gtacggtatt atttttaagg agaaagaatc atgaaaatca aaaaatggct
     2941 tggcctagca gcccttgcta cagtcgcagg tttggctctt gcagcttgcg gaaactcaga
     3001 aaagaaagca gacaatgcaa caactatcaa aatcgcaact gttaaccgta gcggttctga
     3061 agaaaaacgt tgggacaaaa tccaagaatt ggttaaaaaa gacgggatta ccttggaatt
     3121 tacagagttc acagactatt cacaaccaaa caaagcaact gctgatgggg aagtagattt
     3181 gaacgctttc caacactata acttcttgaa caactggaac aaagaaaacg gaaaagacct
     3241 tgtagcgatt ggagatactt acatctctcc aatccgcctt tactcaggtt tgaatggaag
     3301 tgccaacaag tacactaaag tagaagacat cccagcaaac ggagaaatcg ctgtaccgaa
     3361 tgacgctaca aacgaaagcc gtgcgcttta cttgcttcaa tcagctggct tgattaaatt
     3421 ggatgtttct ggaactgctc ttgcaacagt tgccaacatc aaagaaaatc caaagaactt
     3481 gaaaatcact gaattggacg ctagccaaac agctcgttca ttgtcatcag ttgacgctgc
     3541 cgttgtaaac aataccttcg ttacagaagc aaaattggac tacaagaaag cacttttcaa
     3601 agaacaagct gatgaaaact caaaacaatg gtacaacatc attgttgcga aaaaagattg
     3661 ggaaacatca cctaaggctg atgctatcaa gaaagtaatc gcagcttacc acacagatga
     3721 cgtgaaaaaa gttatcgaag aaacatcaga cggtttggat caaccagttt ggtaataaga
     3781 aacagggagg tgggagagag aattccacct cttgcttttg tatagagcac ggagtgtaaa
     3841 gaagactatt cgttcataga aaggtagaga gaatatggtt tttcctagcg aacaagaaca
     3901 gattgaaaaa tttgaaaagg atcatgtagc ccagcattat tttgaggttt tgcgtacctt
     3961 gatttctaag aaatcagtct ttgcccagca ggttggactc aaggaagtcg caaattatct
     4021 gggtgagatt ttcaagcgtg ttggagctga agtggagatt gatgagagct atacagcgcc
     4081 ctttgtcatg gcatatttca agagttcgcg tccagatgcc aagaccttga ttttctataa
     4141 ccactatgac actgtgccag cggatgggga tcaggtctgg acagaggatc cttttacgct
     4201 ttcggtccgc aatggcttca tgtatgggcg tggggttgat gacgacaagg gtcatatcac
     4261 agctcgcttg agtgctttga gaaaatatat gcagcgccat gatgatttac ctgtcaatat
     4321 cagctttatc atggagggag cggaggaatc ggcttcaaca gacctagata agtatttgga
     4381 aaagcatgca gacaaactcc gtggggcgga tttgttggtc tgggaacaag ggaccaaaaa
     4441 tgccttggaa caactggaaa tttctggtgg caataagggg attgtgacct ttgatgccaa
     4501 ggtaaaaagc gctgatgtgg atatccactc gagttatggt ggcgttgtgg aatcagctcc
     4561 ttggtatctc ctccaagcct tacagtctct tcgtgctgcg gatggccgta tcttggttga
     4621 aggcttgtac gaagaagtac aagagcccaa tgaacgagaa atggccttgc tagaaactta
     4681 tggtcaacga aacccagagg aagttagtcg gatttatgga ttggagttgc ctctcttaca
     4741 ggaggatcgg atggcctttc taaaacgttt ctttttcgag ccagcgctta atatcgaagg
     4801 aatccagtct ggttatcaag gtcagggtgt taagactatt ttacctgcag aagccagtgc
     4861 caagctagag gttcgtctgg ttccgggcct agaaccgcat gatgttctgg aaaaaattcg
     4921 gaaacagcta gacaaaaatg gctttgataa ggtagaatta tactatacct tgggagagat
     4981 gagctatcga agcgatatga gcgcaccagc cattctcaat gtgatcgagt tggccaagaa
     5041 attctatcca cagggcgttt cagtcttgcc gacgacagcg gggacaggac ctatgcatac
     5101 ggtctttgat gccctagagg taccaatggt tgcattcggt ctaggaaatg ccaatagccg
     5161 agaccacggt ggagatgaaa atgtgcgaat cgctgattat tacacccata tcgaattagt
     5221 agaggagctg attagaagct atgagtagag atattatcaa gttagatcag atcgatgtga
     5281 cttttcatca aaagaagaga accattacag cggttaagga tgtgaccatt cacatccaag
     5341 aaggggatat ctacggaatc gttggatatt ctggagcagg gaaatcaacc cttgtacggg
     5401 tgattaacct cttgcaaaaa ccatctgcag ggaaaattac cattgacgac gatgtgattt
     5461 ttgatggtaa ggtcaccttg acggcagagc agttgcgtcg taaacgtcaa gatatcggga
     5521 tgattttcca gcattttaac ctgatgagcc aaaagacagc agaggagaat gtagcctttg
     5581 cccttaaaca ctctggactc agcaaggaag aaaagaaggc taaagtagct aagttgttgg
     5641 acttggttgg tttggcagat cgtgctgaaa actacccttc acaactatct ggagggcaaa
     5701 aacagcgtgt ggcaattgcg cgtgccttgg ccaatgatcc aaaaatcttg atttcagacg
     5761 agtcaacttc tgcccttgac cctaagacaa ccaagcagat tttggccttg ttgcaagatt
     5821 tgaaccaaaa attaggcttg actgttgtcc tgattacgca tgaaatgcag attgtcaaag
     5881 acattgccaa ccgtgttgca gttatgcagg atgggcattt gattgaagag agtagtgtgc
     5941 ttgaaatctt ctcagaccct aaacaacctt tgactcaaga ctttatctca acagctacag
     6001 gtattgacga agccatggtc aaaatcgaga agcaagaaat cgtggaacac ttgtctgaaa
     6061 acagtctctt ggtgcaactc aagtacgctg gatcttcaac agacgagcca cttttgaatg
     6121 aattgtacaa gcattatcaa gtaatggcta atattctcta tgggaatatc gaaatcctcg
     6181 atggtactcc tgttggagaa ttggtggtgg tcttgtcagg tgaaaaagca gcgctggcag
     6241 gtgctcaaga agccattcgt caagcaggcg tacagttaaa agtattgaag ggaggacagt
     6301 aagatggaat cattgattca aacctattta ccaaatgtct ataagatggg ttggtctggt
     6361 caggcaggct ggggaacagc tatctaccta accctctata tgacagttct ttccttcatt
     6421 atcggaggct tcttggggct agtggcaggt ctctttctcg tcttgacagc gccaggtggt
     6481 gtcttggaga ataaagtcgt attctggatt ttagacaaaa ttacctcaat ttttcgtgcg
     6541 gttcccttta tcatcctctt ggcaatcttg tcaccacttt ctcacttgat tgttaaaaca
     6601 agtatcgggc caaatgcagc ccttgtccca ctttcttttg cagtctttgc cttctttgcc
     6661 cgtcaggtgc aggttgtctt ggctgaattg gatggcggtg tcattgaggc ggctcaagcg
     6721 agcggagcga ctttctggga catcgtgggt gtttacctat cagaaggtct tccagatttg
     6781 atccgtgtga cgactgtgac cttgatttcc cttgttgggg aaacagctat ggccggtgcg
     6841 gttggagctg gtggtatcgg taacgtagcc atcgcttatg gatttaaccg ctacaatcac
     6901 gatgtgacca tcttggcaac catcgttatc attttgatta tctttgcaat ccaattctta
     6961 ggagatttct tgactaagaa attgagccat aaataaaaaa gagccgtgtg gctcttttta
     7021 actgatcaga ttttctgggc aaatttttta ctcaaggctt gtccaatcaa ggcacccact
     7081 agggctccga tgacaatact tgcgataaat agaaggacag ttccagggtt tggagcgacc
     7141 atgatgcggt cgatatattc ttgggatttt cctcttgcca gaagagtagc catataggct
     7201 ttgggcgcaa tccacataag caagattggt cctgttgtac taaaggcgaa aataatgaaa
     7261 gaaaggaagt tctttgtttt gtccttgtat tttcctaaat gagctactcc atctgctagg
     7321 aggccacaga taattccagg aaggaaggaa ccagcaccgt gtttagttcc caagaaaaag
     7381 agggcaatga caaggccgat agtggtaatg gctccaaagc gcggaacttt tgcgactagg
     7441 atcatataga cgctaccgcc gacaagggca gtaaaggcag gcgcataaaa catgtttcca
     7501 gtttggtcaa agagattgcc caaaaggaca ccaatcccca tgcagaggaa gtaaagaact
     7561 gcaaaaagca gtgtagttaa gatagatttc ttcatgaatt gtctcctgat aattttttca
     7621 caattctcat tttaccatgg tttggtggga ttgtaaatgg atactagaag tttttgaaaa
     7681 cgcttgaaat atgatataat ggtttcatag ttatttttag gggaatatca tgggtagatt
     7741 tttagacttt gtctttaatc gtttcttttt agggatgatt gcgacagcct tcttttggct
     7801 attaacttta gcaggaggga ttatccttgg tctagcgccg gctagtgcca ccttgatgag
     7861 cttatatgca gaacatggtt atagctttcg ggaatacagt ttgaaggagg cttggtctct
     7921 ttacaagcaa aattttatct caagcaacct gattttctat agctttttag gtgtgggtct
     7981 agttttgacc tatggtttgt atctcttggt gcaattgcct catcagacca ttgttcattt
     8041 gattgcgacc cttttgaatg tcctagtagt tgccctgatc tttttggctt atacagtatc
     8101 tttaaaatta caagtttatt ttgccttgtc ctatcgaaat agtctcaaat tatccttgat
     8161 tggcatcttt atgagtctag cagctgtggc taaggttctc cttgggactg tgctacttgt
     8221 agcaattggt tactatatgc ctgccctgct attttttgta ggaattggga tgtggcattt
     8281 ctttatcagt gatatgttgg aacctgtcta tgaaatcatc catgaaaaat tggcgacaaa
     8341 atagaatgaa gcacttttgg ctacatacgc ttctaagaac ctatagttca gtgatgatca
     8401 ttatcattgc gagttttgca atcttactct cttacgctga ctgggattca cgtgaaaagg
     8461 aagcccagag agtagcccag cgtgtaactg ctagaacagt gagtgaaatt gaatattacc
     8521 atagagagtc aacccagata gctcaggctt tagttgaaaa ccaagctcgt attgagggaa
     8581 tctataaata ctttagcctt agcatgccag actattttta ctggcaatta gagcggaaag
     8641 tttcgcctta tatatcagtc tctctgtatg aaaatgttga tgacctctat gttcgaaatg
     8701 attttgtaac tggggtggcc attgcttttc aagattacaa ggaagtctat gtttctacta
     8761 aagacaaacg tagtggagaa aaaatcaggg ctgaggattt caaaccagca ggaaatagtt
     8821 ttgccattcc agtgtcagat ccagtgtcag atcaagactt aggagtgatt tacatctcct
     8881 tggatcctgc tgttttatac catgccattg ataatactag aggtcatact ccgatggcag
     8941 taacagtgac ctcacctttt gatacggaga tttttcatat tggtgagaca gttgataagg
     9001 agagtgaaaa ttggctagtt ggcttaactt ctcatggtta tcaggttcag gtggcagttc
     9061 ccaaaaactt tgttttacaa ggaacggtga ccagctctgc tttgattgtg ggcttgagcc
     9121 ttctctttat tgtcattctt tatctgactt tgaggcagac ctttgctaat tatcaaaagc
     9181 aggtagtgga tttggtggat tccatccaag ctattgccca aggacaagaa ggtcttcgca
     9241 ttgatacgct tgaaaaggat caggaattgc tcctaatcgc ggagacgacc aatgatatgt
     9301 tggatcgatt ggaaaagaat atccatgata tttaccagtt agagcttagt caaaaagatg
     9361 ccaatatgcg agccttgcag gcgcaaatca atcctcattt tatgtataat acgctggagt
     9421 tcttgcgcat gtatgcagtt atgcagagtc aagatgagtt ggcagatatc atttatgaat
     9481 tcagtagtct cttgcgtaac aatatttccg acgaaagaga gaccctcctc aaacaggaat
     9541 tagaattttg ccgtaaatac agctatctct gcatggttcg ctatcccaag tccattgcct
     9601 atggtttcaa gatagatcca gagttagaga atatgaagat tcccaagttt accttgcaac
     9661 cgctggtaga aaactatttc gcgcatggtg ttgaccacag gcggacagat aatgtgatta
     9721 gcatcaaggc tcttaaacag gatggttttg tggaaatttt ggtggtcgat aatggtagag
     9781 gaatgtcggc tgaaaagttg gcaaatatcc gagaaaaatt aagtcagaaa tattttgaac
     9841 accaagccag ctacagtgat caaaggcagt ctatcggaat tatcaatgta cacgagcgtt
     9901 ttgtgctcta ttttggagac cgctatgcca ttacgataga gtctgcagag caagccggtg
     9961 ttcagtatcg tattacaatt caagatgagt agaaagggag aaaatgtata aagtattatt
    10021 agtagatgat gagtacatgg tgacagaagg tctgaagcgt ttgattccct ttgataagtg
    10081 ggatatggag gtcgtcgcaa cagccagtca tgccgatgaa gctctagaat atgttcagga
    10141 aaatcctgtc gatgtcatca tttccgatgt caatatgcca gacaaaacag ggcttgatat
    10201 gattcgggag atgaaagaga tcttaccaga tgctgcctat atcctgctct caggttatca
    10261 ggagtttgat tatgtaaaaa gagcaatgaa ccttagtgtg gtggactatt tggtcaagcc
    10321 tgttgataag gtagagctgg gaaatctgct ggagaagatt gcaggtcagc tcggcgagag
    10381 agggaagaaa agtcagactc ttagtcaaga attagacgag gctggatttg ttagttattt
    10441 aggggataag gagaattggt ggataggtct atccaaggaa aaacaaggtt ccttcaccat
    10501 tccctactat gtcttgggcc aagactggca gattttcatt tctgaccaac ccctagatgg
    10561 tttagtcgtt acaccttttg aagctcctta tcaagaacac tttgaacgct ggaagctgaa
    10621 tgctgagaaa accctctttt acggttctgt aaatttgcag cagtctgaga gtctctttgc
    10681 ctattacgaa ccgatttata gggttatcat tcagggaaat ctcaatcaaa tcgtagaaga
    10741 gttaaatctc ttggagaagg tagttcttga aaatacgccg cgtgttccga ttactaaaca
    10801 gctttttatt cagtttgtca tggatgtttt ccatttattt gaacatctca aagctgatga
    10861 cctgacagac atcgtcaaaa ccattcatgc tattcaatcc ttcgatgaat tggttcctta
    10921 tatcaaggaa actctgagca gctttttcgg tcaataccgt atgaatgaaa atgtggtcag
    10981 tgtgctggaa gtcattggtc gtgattacca aaaagagctt tccctcaagg atatcagtaa
    11041 ggctctcttt atcaatcctg tctatctagg gcagttgatt aagcgtgaaa ccgattcgac
    11101 ttttgcagag ttactaaaca aacaacgtat taaggctgcc caacaacttt tgctttcaac
    11161 tagtgacagc atcgaagata tttgttatgc tgttggttac agtaaccttg gatatttcta
    11221 taaagttttc cgaaaattgt gcggaaaatc gccaaaagcc taccgaaaac aggtagaaac
    11281 tatactataa gatttgtatt cctttacaaa atgtgctata atataaatat tattattagg
    11341 aggttctatc atgaaaaaga aaccaattta tctatgggtc ttgctaatct tgtctgccct
    11401 tatttcagtt ccgtctctgt ttggaattgt gagtcccttg cctagtaaag aagcccttcg
    11461 tgctgctcag aaacaagttg caggggtcaa tgctcagcaa ttagaagacc agcttaatta
    11521 tacctataga gtagcagaag catctcattc tatttttaat gttgctttga ttgtgctatc
    11581 tactatttta gtggtcgtag cgattgtctt ccttgttcgt aaaaatttgc aatatgcaaa
    11641 ctatacttac gttggctatg ttttgttagc tattattggt tcgatttatg gctatgttgg
    11701 tttacaagac gctgtgcagt tggttcaaga tgagagcatg cgtttgacag tgagtattgg
    11761 ttcaaaagct gttagcattt tctatatcgt catcaatgtt ctcttcctag cccttgtctt
    11821 ctataagatg tggcgacaac agaaagcctt ggcagaagaa gaggaaacag aagagcttgc
    11881 ctaagtattg acaaaaaaga actatcaaga tacaatcttg gtagttgttt tttgattata
    11941 gataaaatca tggaggtaca tatgaagaca atttctttag tttatattag tttgagtggc
    12001 aacactgaaa gttttgtgac gcgcttgaaa gactatctct tgtcccagta caaagggatt
    12061 gaggttcaaa agattcatat caaggatttg gtcaaggaag gcaaaaattt ctacgaaatg
    12121 gaccatccct atgttgcttt tttgccgacc tatctcgaag gtgggaatgg cgtggataac
    12181 ggagatgttg agattttgac gacaccggtg ggagatttta ttgcctatgg taacaatgct
    12241 agtaagtgtt ttggtgtggt tgggtcagga aatcgtaact ttaataacca atactgccta
    12301 acagccaagc aatacagtca acgctttggc tttcctgtat tggctgactt tgaaatgcga
    12361 ggcatgctgg aagatatcaa acatgtcgca gctattatcg cagatttgta tgagttggaa
    12421 aaggagaatt aatactcaat gaaaatcaaa gagcaaacta ggaagctagt cgcaggttgc
    12481 tcaaagcaca gctttgaggt tgcagataga gctgacgtgg tttgaagaga ttctcgaata
    12541 gtataaagtt cttataagag gtggttgaag ttgttcaact gttttttgag tataaacagt
    12601 ctttaagaac gtaaaaaagc ataagttcag gtcttcaaaa ccttgttctt atgcttttta
    12661 tcatagaaat atttgctgaa ttttctcttg aaatgaaacc ttatcttcat ctcgcgcttg
    12721 tcccttaccc tgcttttccg attgcgagtg cgtagataaa gaagatggcg aagccaaaga
    12781 ggaaaatcaa tcccgcaatg gcaaggtgtt tgagccaaga aggaagattt ttgttttcac
    12841 gtgttaccaa ggcgtaattc aaaagagcaa agaaaggtgt tgtcaggaaa gagccaatca
    12901 tggcaaagcg gagcatggtt gaaacctgac cagcgaagaa cttgataatg atgataccga
    12961 tgatagcagt gatggtcatc cagatgttca aagatttacg attatcctct ttttgactga
    13021 ttagcagtcg gagagattcc tgattaacgc gagaatagcc atcgataaca gttataactg
    13081 ttccaaagat acagaggaag gcaataaagg taatcaagta acgggaccat tcgccaagaa
    13141 cagaggcata catgcccacg aattgagaga tgtatttggc tgaagcagct tcaaccgcct
    13201 gccctgtagg atactgaatc agtgctccca gtgccacaaa gaagacggct aggatagctg
    13261 ttccaatata accagtgtta aagtcaaaca gagcgtcttc tgtgttaaag ttgacggtct
    13321 ttctcttttc agctgaccaa agtgaattga tggctgaaat ttcaatagga gccggcatcc
    13381 atcctaggag ggagacgatg aagggcagag ctgccatttg ccaaggtgtc ttctcgacaa
    13441 aatcagaact gtattctgga tgcttgaccg ccgcaatgat aactgcaaga acagtcgcaa
    13501 tggttaaggc agacataatc catttgacca tgccgtctaa aagtttgtag cctccaaaga
    13561 gtagcatagc ccaaatgatt gcaacgagaa tgagggacca ctgagtaatg ctaagtccaa
    13621 tcattgggaa ggcactggcg atgatagctg agcacagaat ggcaacacca gccgtgttga
    13681 ccatagccga aaagacattg aggataaaga aaatccagag atagagtttt cctttttcgg
    13741 cataaccttc aaccaaagtc tttccagtat cagctgtgta ttcagcacca aaacggaaaa
    13801 atggatattt aaagacattg gctaagatga ccaagagaag tagagaccaa ccgtaagaac
    13861 cgccagcttg agttgaggat acaatgtggg aacctccaac agcggcagtt gccattagga
    13921 ttccaggtcc cattgctttt agcttgcttg cccaggttga ttgatttaag gaaatagctt
    13981 gcgacatgat aaatactcct ttttgaattt tcagaataat ctgtatatgt aatctatatt
    14041 ctacaaaaaa ttttggaaaa atgcaagagt attttgaaaa aagatagaaa atttacaaag
    14101 aaaatctgaa ataactgtta tgatataga
//
LOCUS       DAVLXF010000045        13778 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLXF010000045 DAVLXF010000000
VERSION     DAVLXF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.045"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            103..1584
                     /locus_tag="VYO12_001306"
     CDS             103..1584
                     /locus_tag="VYO12_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1587912.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1946..2947
                     /locus_tag="VYO12_001307"
     CDS             1946..2947
                     /locus_tag="VYO12_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1587913.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3098..4015
                     /locus_tag="VYO12_001308"
     CDS             3098..4015
                     /locus_tag="VYO12_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1587914.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4026..4916
                     /locus_tag="VYO12_001309"
     CDS             4026..4916
                     /locus_tag="VYO12_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1587915.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4945..6561
                     /locus_tag="VYO12_001310"
     CDS             4945..6561
                     /locus_tag="VYO12_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1587916.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVENLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6571..7890
                     /locus_tag="VYO12_001311"
     CDS             6571..7890
                     /locus_tag="VYO12_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1587917.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8100..8172)
                     /locus_tag="VYO12_001312"
     tRNA            complement(8100..8172)
                     /locus_tag="VYO12_001312"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8137..8139),aa:Lys,seq:ctt)
     gene            complement(8437..9708)
                     /locus_tag="VYO12_001313"
     CDS             complement(8437..9708)
                     /locus_tag="VYO12_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1587918.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            9978..10178
                     /locus_tag="VYO12_001314"
     CDS             9978..10178
                     /locus_tag="VYO12_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587919.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            10382..10561
                     /locus_tag="VYO12_001315"
     CDS             10382..10561
                     /locus_tag="VYO12_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587920.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10698..10877
                     /locus_tag="VYO12_001316"
     CDS             10698..10877
                     /locus_tag="VYO12_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1587921.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            10914..11366
                     /locus_tag="VYO12_001317"
     CDS             10914..11366
                     /locus_tag="VYO12_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1587922.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11657..12127
                     /locus_tag="VYO12_001318"
     CDS             11657..12127
                     /locus_tag="VYO12_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1587923.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFIMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12183..12725
                     /locus_tag="VYO12_001319"
     CDS             12183..12725
                     /locus_tag="VYO12_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587924.1"
                     /translation="MLEKVKQFFRSRSAKTEPSVDILPRNRFEDLDFERVLKSGARRL
                     VNEEGRYAEDGKITELEFPEDFAEFEFLVGFKTEEEEQFQQLLARLNSIDNAIQSYLE
                     SEMQQPIPQYAKDLGYTQKRWEKTFYFHPWILSGEENPPNLRYVADYVNDEFTVYFAK
                     KHGRWQAYWDAECQKVIEES"
     gene            12775..12939
                     /locus_tag="VYO12_001320"
     CDS             12775..12939
                     /locus_tag="VYO12_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEV1587925.1"
                     /translation="MELLNSRMDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPG
                     GGKSVRENLI"
     gene            13049..>13778
                     /locus_tag="VYO12_001321"
     CDS             13049..>13778
                     /locus_tag="VYO12_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1587926.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATI"
BASE COUNT         4493 a         2218 c         2827 g         4240 t
ORIGIN      
        1 taaaagattt taggaaaagt cgttatatca tgctatacct attctttgtg gtataattgc
       61 aagaggttta atccgataat ttataaagag agaaagatat tcatgagaga tttattatct
      121 aaaaaaagtc ataggcaatt agaattatta gaattattat ttgaacataa acgttggttt
      181 catcgttctg aactagcaga gttattaaat tgtacagaac gtgcagtcaa agatgatcta
      241 tcccatgtta aatctgcttt tcctgacttg atttttcatt cttctactaa tggtatacgc
      301 attattaata ccgatgatag tgatattgaa atggtttacc atcatttctt taaacattca
      361 actcattttt cgattttaga attcatcttc tttaatgaag gttgtcaagc tgagagtatt
      421 tgtaaagaat tttatatcag ttcatcttcg ctctatcgta ttattagcca aatcaataaa
      481 gtgattaaaa ggcaatttca atttgaagtc agtctgaccc ctgttcaaat cattggaaat
      541 gagagagaca ttcgttactt ttttgcacaa tatttttcag aaaaatatta tttcctagaa
      601 tggccatttg aaaatttttc atcagagcca ctatctcaat tgttagaatt ggtttataag
      661 gaaacaagct ttccaatgaa tttgtcaacg catagaatgc taaagttgct cctagttacg
      721 aacctatata gaataaagtt tggtcatttt atggaagtag ataaagattc ttttaacgac
      781 caaagtttgg attttttaat gcaggcagaa ggaatagaag gtgttgctca gagttttgaa
      841 tcagaataca atatctcttt agatgaagaa gttgtttgcc aattatttgt gtcttacttt
      901 caaaaaatgt ttttcataga tgaaagtctc tttatgaaat gcgtaaaaaa ggatagttat
      961 gttgaaaaat cttaccatct attgagtgat tttattgatc agatttcagt caagtatcag
     1021 attgaaatgg agaataagga taatctgatt tggcatctgc ataataccgc acatctgtat
     1081 cgtcaagagt tgtttactga gtttatttta tttgatcaaa aagggaatac aatcaggaac
     1141 tttcaaaata tttttccaaa atttgtttca gatataaaaa aagagctttc tcactattta
     1201 gagactcttg aggtttgttc tagttcaatg atggtaaatc atctatctta taccttcatc
     1261 actcatacca aacatttggt gattaatttg ctacaaaatc agcctaaact aaaggtttta
     1321 gttatgagta attttgatca gtatcatgca aaattcgttg cagagacact ttcttattac
     1381 tgtagcaata attttgaact ggaagtttgg actgaattag aattatcaaa ggaatcttta
     1441 gaagattcac cttatgatat cattatttct aattttatta ttcctcctat tgaaaataag
     1501 agactcatct attcaaataa tataaacacg gtctcactca tatacttgtt aaatgccatg
     1561 atgtttattc gattagatga gtaaccaaaa ataacaaaaa aacgctattt cgcgtttgaa
     1621 ctgtacctca aaagttagac agaaaaaatc taatttttgg ggtgtttttg ttaagaaaag
     1681 accttcaatc atttaagcaa aatgtacatc tagatttata gccctctaaa actcttgctt
     1741 tttttgtaga agatacgaaa aaagccataa aaccaaggct tttcctgttt tatgtagatg
     1801 tttacacatg aaaattgaag tattttcaaa agctaattgg agtgatttta ttcatgatgg
     1861 gataaatgat ctggccaaaa atgattgaga aatagttgct taaaaagcat ttgcatgata
     1921 ctatatattg aaaggaggtg ataatatggt agaacaaaga aaatcaatta ccatgaaaga
     1981 tgttgcttta gaagcaggag ttagtgttgg aactgtttca cgtgtaatta ataaagaaaa
     2041 aggcattaaa gaagtaactt tgaaaaaagt ggaacaagcg attaaaactt tgaattacat
     2101 tccagattac tatgctagag gaatgaaaaa aaatcgaaca gaaacgattg caatcattgt
     2161 accaagtatc tggcatccct tcttttcaga atttgctatg catgtggaaa atgaagtcta
     2221 taagagaaat aacaaattac tcttatgttc tataaatggt acaaatagag agcaagacta
     2281 tctggagatg ttgcgtcata ataaagttga tggagtggtt gccattacct atagtccaat
     2341 tgaacattac ttgacgtcag gaattccctt tgttagtatt gaccgcacat actcagatat
     2401 tgccattcct tgtgtttcat cagataatga tgcaggtgga agagaagcag ctaaacaatt
     2461 aattagtaaa ggatgtcagc atttagcctt tgtgggcgga cataatacaa ccattaatga
     2521 aaccaaacgt cgtagaattt catttgaaaa gtatgtccaa gagcataaaa tacctcttag
     2581 tatttttgat ttggatgaga cagttactga ctattatgga aagctagaga gtttcttgga
     2641 agaaaattct tctatagatg gaatttttac aatcaacgat tttacagctt tggatgtgat
     2701 tgaaatacta gaaaaaaatg gcaaacgtat tcctcatgat gtccaaatta ttggatacga
     2761 tggaattaaa atggctggag atagagatta tctactttca actattgaac aaccattgga
     2821 agaaatggca aaagaagcgg ttcgtatttt gtttgatata attgatggaa agactgttaa
     2881 tttgcagaca atcctaccag taaaatttgt tgaaggaaaa acaacaaaaa atgaaaataa
     2941 gtcttgacag aaagcgctat caatgataga atgaattcag ataaaaagat ttatttttaa
     3001 aacaaaaatg aaacgtttca aaaaaagaaa taaaaagaca gcgccaagcg ctatctttta
     3061 tagaaaaaat gaaacgtttc aaaaaaagga ggttgctatg aatagcaaag cgaagcaagt
     3121 ttctctttgg gaaagaatca agaaacaaaa actcttgtta ttgatgactg tccccggttt
     3181 agttttaacc tttatcttta aatacatccc tatgtatggg gttttaatcg catttaaaga
     3241 ttacaatcct ttaaaaggaa ttttagggag tgattggatt ggtttttctg agtttacaaa
     3301 attcatatcc tctcccaact ttggtatctt gttagccaac acattaaaat taagtatcta
     3361 tggtttattg cttggctttt taccaccaat cattcttgcg attatgctca atcaactctt
     3421 gagtgaaaaa gtcaaaaaac gaattcagct cattttatac gcaccaaact ttatctcagt
     3481 cgttgttatt gtcggtatga ttttcctctt cttttcagtg ggaggaccaa tcaacaattt
     3541 tctttctatg tttggaatga aggctgactt cttgacaaat ccagacttct ttagaccctt
     3601 atacatcttt agtggtatct ggcaaggaat gggctgggct tcaacgctct acacggcaac
     3661 attggtaaat gtagatccag ccttagtaga agcagctcga ctggatggag ccaatatctt
     3721 ccaacgaatc tggcacattg atattccagc tcttaagcct attatggtta tccaatttgt
     3781 tttagctgca ggtggaatta tgaatgtcgg atatgaaaaa gcattcttga tgcagacatc
     3841 gttaaatttg ccaacttctg aaattatctc gacatatgtc tataaagttg gtcttgtatc
     3901 aggagactat tcttactcaa cagcggttgg tttgtttaat gcagtgatta acgtagtatt
     3961 gcttgttgca gttaaccaaa tcgttaaacg catgaataat ggtgaaggaa tttaaggagg
     4021 aaagtatgaa aaattcgatt atggatacaa aatttgatag acgtatctta ctcttaaata
     4081 aaatcattat tgtctttatc gttttgatga ctttgcttcc tttactttat atcgtcgtag
     4141 catcctttat ggatcctaag gttctggtta gtagagggat tagctttaat ccagccgatt
     4201 ggactgtaga aggttaccag cgtgtattca gtgaccaatc tattctaaga ggttttatca
     4261 attctctact atactctttt ggatttgcag ctttaacagt attgctatct gtgtttacag
     4321 cttatcctct ttctaagaaa gacttggttg gacgtcgttg gattaactac ttcttgattg
     4381 tgactatgtt ctttggtggt ggtttagtcc caacttactt gctcgtaaaa gaattgggaa
     4441 tgctcaatac tccatgggct atcattgttc caggtgctgt caatgtttgg aatattattc
     4501 ttgctagggc ctatttccaa ggattgcctg aagaattagt tgaagctgct gtcattgatg
     4561 gagcgaatga cttgcagatt ttctttaaaa tcatgcttcc tcttgcaaaa ccaattatgt
     4621 ttgttctctt cctctatgct tttgtaggac agtggaactc atactttgat gcaatgattt
     4681 atatcaagga tccaaacttg gaaccattgc aacttgtact tcgtaaaatt ctcattcaga
     4741 gccaaccagg tcaagacatg attggagcac aagcggctat gaatgaaatg aaacgtttag
     4801 ctgaattgat taaatacgca actattgtca tttccagctt gccattgatt gttatgtatc
     4861 cattcttcca aaaatacttt gataaaggaa ttatggctgg ttcacttaaa ggataaaaaa
     4921 agaaaaaata aaaggagttt tctcatgaaa ttcaaaacat tctcaaaatc agcagttttg
     4981 ttgacagcta gtttagcagt acttgcagcc tgtggctcaa aaaatacagc ttcaagtcca
     5041 gattataagt tggaaggtgt aacattcccg cttcaagaaa agaaaacatt gaagtttatg
     5101 acagccagtt caccgttatc tcctaaagac ccaaatgaaa agttaatttt gcaacgtttg
     5161 gagaaggaaa ctggcgttca tattgactgg accaactacc aatccgactt tgcagaaaaa
     5221 cgtaacttgg atatttctag tggtgattta ccagatgcta tccacaacga cggagcttca
     5281 gatgtggact tgatgaactg ggctaaaaaa ggtgttatta ttccagttga aaatttgatt
     5341 gataaataca tgccaaatct taagaaaatt ttggatgaga aaccagagta caaggccttg
     5401 atgacagcac ctgatgggca catttactca ttcccatgga ttgaagagct tggagatggt
     5461 aaagagtcta ttcacagcgt caacgatatg gcttggatta acaaagattg gcttaagaaa
     5521 cttggtcttg aaatgccaaa aactactgat gatttgatta aagtcctaga agctttcaaa
     5581 aacggggatc caaatggaaa tggagaggct gatgaaattc cattttcatt tattagtggt
     5641 aacggaaacg aagattttaa attcctattt gctgcatttg gtatagggga taacgatgat
     5701 catttagtag taggaaatga tggcaaagtt gatttcacag cagataacga taactataaa
     5761 gaaggtgtca aatttatccg tcaattgcaa gaaaaaggct tgattgataa agaagctttc
     5821 gaacatgatt ggaatagtta cattgctaaa ggtcatgatc agaaatttgg tgtttacttt
     5881 acatgggata agaataatgt tactggaagt aacgaaagtt atgatgtttt accagtactt
     5941 gctggaccaa gtggtcaaaa acacgtagct cgtacaaacg gtatgggatt tgcacgtgac
     6001 aagatggtta ttaccagtgt aaacaaaaac ctagaattga cagctaaatg gattgatgca
     6061 caatacgctc cactccaatc tgtgcaaaat aactggggaa cttacggaga tgacaaacaa
     6121 caaaacattt ttgaattgga tcaagcgtca aatagtctaa aacacttacc actaaacgga
     6181 actgcaccag cagaacttcg tcaaaagact gaagtaggag gaccactagc tattctagat
     6241 tcatactatg gtaaagtaac aaccatgcct gatgatgcca aatggcgttt ggatcttatc
     6301 aaagaatatt atgttcctta catgagcaat gacaataact atccaagagt ctttatgaca
     6361 caggaagatt tggacaagat tgcccatatc gaagcagata tgaatgacta tatctaccgt
     6421 aaacgtgctg aatggattgt aaatggcaat attgatactg agtgggatga ttacaagaaa
     6481 gaacttgaaa aatacggact ttctgattac ctcgctatta aacaaaaata ctacgatcaa
     6541 taccaagcaa acaaaaacta gaggttgatt atgggagata agaaatacac agtagaaaaa
     6601 gccaatcgtt ttatagcaga aaataaacat ctcgttaata ctcaatataa gcctgaagaa
     6661 catttttcag ctgagattgg ttggatcaat gatccaaatg gatttgtcta ttttcgtgga
     6721 gaataccatc tcttttatca attctatcca tatgatagtg tttgggggcc tatgcactgg
     6781 ggacatgcta aaagtaagga cttggtgact tgggagcact tgccagtggc acttgctcct
     6841 gaccaagatt atgaccgaaa tggttgtttc tcaggctctg ccattgtcaa ggatgatcgc
     6901 ctctggctca tgtacactgg acatatcgaa gaagaaaccg gtgtccgcca agtgcaaaat
     6961 atggcatttt cagatgacgg gattcacttt gaaaagattg aacaaaatcc agttgcaact
     7021 ggatcagact tgccagatga gttgattgct gctgattttc gtgatccaaa actctttgaa
     7081 aaagatggac gctattactc cgtagtagct gccaaacaca agaataatgt gggctgtatc
     7141 gttctactag ggtccgataa cctagtagaa tggcagttcg aatccatctt tttaaaaggg
     7201 gtagaacacc aaggctttat gtgggaatgc ccagattact tcgagttaga tgggaaagat
     7261 tgccttatta tgtcacccat gcgttatcag cgtgagggag actcatatca taacatcaac
     7321 tcatcgcttt tgttcacggg taaggtagat tggggagaaa aatgttttat cccagaatca
     7381 gttcaagaaa ttgatcatgg ccaagacttc tatgcgcctc aaacattgtt ggacgatcaa
     7441 aatcgtcgta tcctgattgc ttggatgcag acatgggggc gtacccttcc aacccatgac
     7501 caagaacaca aatgggcatg tgccatgact ttacctagaa ttttaagatt ggaagatggc
     7561 aaactaagac aattccctgt taaaaaaggc caatatcaaa tccaaataga taaagattgt
     7621 cattaccact taggaaatga tatagattat cttgaatttg gttatgatag taatgcgcag
     7681 caagtttaca ttgatcgtag ccatcttatt caaaaaattc taggtgaaga agaacaggac
     7741 actagtcgac ggtatgtaga tattgaagct aaagaattgg aagttgttct agataaaaat
     7801 tccatcgaga tttttgtcaa tcaaggtgaa gcaagcttga ctgcaactta ttacttaacg
     7861 gtgccagctg agctatcacg aattgattaa aaattaagtt atttctccta aagaaaaagt
     7921 tctctttcta aaatagtgga aagaggactt tttgtgtttt gggtatataa gcttagttta
     7981 tggtatttgt aaaattggtg ttggattatg atttaagcta gttttctaaa gaatttgaaa
     8041 aaaattttat ttaagcaaaa aaaacccttg gttccaaggc ttttcctgtt gtatttatat
     8101 gccccctaca gggatcgaac ctgtgaccca cggattaaga gtccgctgct ctgccagctg
     8161 agctaaggag gcaaagaaaa aagctgtatt ggtgccgaaa cttcacggtt tgtattgaac
     8221 ccgcgcaatt aagcaggtgg gcaactcgct ctaactgaag ctgtttccgt gtgagacggc
     8281 ctacatgctg ttagaagact tttgtttccc taataataca aaaaatagtc ggtcaacact
     8341 taagtgtgaa gtcgtacacc acagcgtttc tatacttata tgataccact ttttcaaaaa
     8401 aaatcaagag gaaagtgcaa ttttttgaaa aggatttcag atttttcgca aacggtcgat
     8461 agcttccttt ctttgagcca aagcccgttc atatttacca gtatcttctg cttggaaata
     8521 gtgatgatta cgaatttttt ctggcagata gtcttgcttg acccaatttc caggatagtt
     8581 gtgtggatag agatagtctt gggcattccc cagttccttg cttccactgt agtgcccatc
     8641 acgtaggtgt cgcggaatag gcaagtgccc tgatgttttg aggtcagcaa gtgccttatc
     8701 catagctaca taggctgagt tggattttgg agaaagggcc aaatcaatca cgacattggc
     8761 aatgagaatg cgggcttctg ggaacccaat cttctgggca gcatccagag cagtcacggt
     8821 atgaatctgg gcctcaggat tggctaagcc gatatcttca taggcgataa cagtcaagcg
     8881 acgagcgaga ctaggcagat ccccagcctc aatcaagcgg gcagtatagt ggagactggc
     8941 atccacatct gagccacgaa tagacttttg cagggcagat agaacatcat agtgaccgtc
     9001 tccatccttg tccatagtga tgtagcttct ctgaagacta ttttccatga tgtctaaggt
     9061 gatgtggcga atgccctcgt cattctcagg ggtagagaga acagctaagt ccagtgagtt
     9121 aaaggcagag cgaaggtctc cgtttgtaga ggttgcgatg aaatccagcg catcctcatc
     9181 tagttctatt ggaaaatcaa aaccacgttc agggttactt agagctatct gcagggcctc
     9241 tttgacgtct tggttagaca gaggttccaa ctcgaaaatt tgaactcggc tacgaatggc
     9301 aggagtgaca gagaagaaag gattttcagt cgttgctcca atcatgatga ccagtccact
     9361 ttccaagaga ggcaagagga agtcttgctt ggtcttatct agtcgatgaa tttcgtctag
     9421 caataggacg agaccaccag aaaatttagc ttcttccgag atttcttgca gtcgcttttt
     9481 actatcaact gtcgcattga aagttcgaaa ggcatacttg gtcgttccag cgatggcaga
     9541 ggcaatactg gttttgccga ttccaggagg gccatataga atcatggagg acaggcggtt
     9601 ggcttccacc atgcggcgga tgatttttcc aggtccgacc agatgctcct gaccgatgac
     9661 ctggtcgatg gttttagggc gcatgcgaag cgcgagattg tctggcatag cagtcctttc
     9721 tagcgtggat ttgagtagaa tgactcatct actatatctt actatgtcca ttctagccta
     9781 ttagttgtga tatttcaatg gttttgtaat aatcagggag tgagatagaa tcttttttac
     9841 tatttaattt aatgaggaga ggtatcagca tgagagattt gattttcctt aaagctcaaa
     9901 ataggatagt tgagcctaaa gaagtggaag tgctgacttg agaaataata ttgctgaatg
     9961 tcattgtatg aactataatg aaaagagagg tgataagcaa tggaaatgat ggaccaagtc
    10021 aggaaatttt gatttttaca aaacaaattc gtcattggat tcttagtgat caagttattt
    10081 cagggaaaag aaaacttttc ttccgggaag atactccaaa agagatttta gatttgtacg
    10141 aaaacattaa atctaaactt gattttgctt accaataagt ttattctaat aatgggttga
    10201 aaaaatatga aaaatagaat aattgatgtt tttgaagtag taaatcgcat ccttgtaata
    10261 actgttgaaa atcctgattt cgaagacttg agagttaatc atactcaatg aaaatcaaag
    10321 agcaaactag gaaactagcc gcaggctgta cttgagtacg gtaaggcgac gttgacgtgg
    10381 tttgaatttg attttcgaag agtatcagtt tgtaaaaatt ggagataaaa aatatagagt
    10441 acgcagtgtt cctatgattc attcaactcc gcctcaatct gtcttagatc gagatacttt
    10501 tacaattgac tatacagatg atgaattgct cgataaagaa gcagttttta ctactcatta
    10561 gaaaaatagg tctgaataaa ttggcttccc ttagaaccac atctaggggc ttttatgtat
    10621 aatagaataa taagtatcac tcctttacaa tacgtgtaac acgtgttata atggtgttgt
    10681 caggaggtaa aagcgctatg cctatgacac aaaaagagat ggttaaactc cttacggccc
    10741 atggttggat aaagactaga ggcggtaaag gctcccatat taaaatggag aagcaagggg
    10801 aaagacttat cacaatccct catggtgagc tgaataagta cactgaaaga gggataagaa
    10861 agcaagctgg gttgtaaaat tcagcccctg ttttttgata tggaggtagt gaaatgttag
    10921 ttacgtatcc agctttattt tattatgacg acacagacgg aacagaagcg acttattttg
    10981 tccatttccc agattttgaa tactcagcta cacaaggaga ggggatttct gaggctttgg
    11041 ctatggggtc ggagtggcta gggataactg ttgcagattt gatagagagt gatggagagt
    11101 tacctcagcc atcagatatc aatagcttgt ctttaattga taatgatcct tttaaagatg
    11161 atgaagattt cgtgtcaacc tatgaccttg ataaatcttt tatttctatg gtatctgttg
    11221 atgtatcaga atacctagga agtcaggaac ccataaaaaa gactttgacc ataccaaaat
    11281 gggcagataa gttgggacga gaaatgggac ttaacttttc tcagactttg acagacgcta
    11341 ttgcagataa gaaagttcaa gcctaagatt aagcgtggta tcgtggtaat agtcaataaa
    11401 aaagcacgtc cacttgtgct agttactcgc ctattgattt tatagattta ttagcccttt
    11461 cgagggcttt ttatattgat ttttatgaga aatgaagaaa tcagactttt aagaaaatca
    11521 ctgataccaa gggtttaaat gaggtaatat ggtataatta ggacataaaa taattttgtg
    11581 gtaagatggt agtatctatt ttagcatatt tccgagcaat cggggcgatt aaagagtcgc
    11641 atagaaagag gacaaaatgg caacatacgg atttttagat gttttagagg aagagttgga
    11701 caagaacttt ccctttgact ttgagattag ctgggacaaa cgcaaccatg cggtagaagt
    11761 gagttttcta ttggaagcgc aaaatgctgc aggtgtggag atggtggatg aagacggaga
    11821 ggtttcatca gatgacattc tttttgagga agcggtcctt ttctacaatc ctgccaagtc
    11881 aacagtcaat gaggaagact atttgactgt tattccttac ctgcctaaaa aaggattttc
    11941 ccgtgaattt ttagcttatt ttgcgctttt ccttaaagat actgctgagg ttgggctaga
    12001 tgtcctcatg gactttttgg aagacccaga agcagaggaa tttatcatgg aatggaacca
    12061 agaagtcttt gaagaaggaa aagttggctt ggaagaggga gaattttatc cttatccaag
    12121 gtattagaaa agattctaag tggacatttt taaataatga attcaaggag aagggggaga
    12181 ttatgctaga aaaagtcaaa cagtttttca ggagcaggtc agctaaaact gagccgtcag
    12241 ttgatattct tccccgcaat cgctttgagg atctagattt cgagagagta ttgaagtcag
    12301 gagctcgtcg ccttgttaat gaggaaggac gctatgctga ggatgggaaa atcacagaac
    12361 ttgaatttcc tgaagatttt gcggaatttg aatttctagt tggttttaag acggaggaag
    12421 aagagcagtt tcagcaacta ttagctcgtt taaatagtat tgacaatgct attcagtcct
    12481 atttggaaag tgagatgcaa caacccatcc ctcagtatgc caaggatctc ggctatactc
    12541 agaagaggtg ggagaaaacc ttttacttcc atccttggat attaagtggt gaggaaaacc
    12601 cgcctaatct tcgctatgtt gcagattatg taaatgatga gtttacggtt tattttgcta
    12661 aaaaacatgg cagatggcaa gcatactggg atgcagagtg ccaaaaagta atcgaagaaa
    12721 gctagctcgt attttcctag agagtaattg ttatcataga aaagagtaaa tatcatggaa
    12781 ttattgaata gcagaatgga tttccaaggt tgcaagattg ccctcttttg tggggataag
    12841 ctactgacga ttttgcgtga tgacaaggac gatatccctt gtcccaacat gtgggagttg
    12901 ccaggtgggg gaaagagtgt cagggaaaat ctgatatagt tttgcattaa aagaatatcc
    12961 tttcaaggtt attctttttt aattttgtcc caaaataatt gactttgtcc caaaaatata
    13021 gtattatttg tataaggagg aatgaatcat gaaaaaatca gatgttcttg atttaataaa
    13081 gtatcattat gagggcagag agacagaatt tagaaatcaa tccatagcaa ttgctcggaa
    13141 tttcaataaa catggtgata cacaaatagc acaatatatt atggggttaa tgtctcaaag
    13201 tgatagattc atgccacaaa tagaaaatcc tagtgagtat ttgactcctg ctaaactaga
    13261 tattggtcct ctcccattgc ctctttcaat tatgaatgat ttaaaaggaa taataaacgc
    13321 tgtcaatcat catattggaa taaataaatt tttatttgtg ggatctccag gtactggaaa
    13381 aactgaaagt gtaaagcagg tggctagact tattggcaag gaattacttg tagtagattt
    13441 cagtcactta gtagatagta agctagggca aacagtcaaa aacttagcaa ctctttttaa
    13501 tgaaatcaat aatcttcctt tcaagcaaaa ttatatcatc ctatttgatg aaattgattc
    13561 catagtgttg gatagggtta atcagaatga tttaagagaa atgggacgag tgacttctgc
    13621 ctttttaaag gagttggata ggctgtcacc agaaattgta ttaattgcaa caacaaatct
    13681 ttttgaaaat cttgataaag cagttacgag aagattcgat gccataattg actttgaccg
    13741 ttatactgat gaagataaag tagaagtagc tactatta
//
LOCUS       DAVLXF010000046        13752 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLXF010000046 DAVLXF010000000
VERSION     DAVLXF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13752
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.046"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(116..2374)
                     /locus_tag="VYO12_001322"
     CDS             complement(116..2374)
                     /locus_tag="VYO12_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1587927.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2603..2833)
                     /locus_tag="VYO12_001323"
     CDS             complement(2603..2833)
                     /locus_tag="VYO12_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1587928.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3023..3706
                     /locus_tag="VYO12_001324"
     CDS             3023..3706
                     /locus_tag="VYO12_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1587929.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3706..4440
                     /locus_tag="VYO12_001325"
     CDS             3706..4440
                     /locus_tag="VYO12_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1587930.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4594..5451
                     /locus_tag="VYO12_001326"
     CDS             4594..5451
                     /locus_tag="VYO12_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1587931.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAMDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5593..6465
                     /locus_tag="VYO12_001327"
     CDS             5593..6465
                     /locus_tag="VYO12_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1587932.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6747..7430
                     /gene="rpiA"
                     /locus_tag="VYO12_001328"
     CDS             6747..7430
                     /gene="rpiA"
                     /locus_tag="VYO12_001328"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1587933.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7444..8655
                     /locus_tag="VYO12_001329"
     CDS             7444..8655
                     /locus_tag="VYO12_001329"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1587934.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8657..9199
                     /locus_tag="VYO12_001330"
     CDS             8657..9199
                     /locus_tag="VYO12_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV1587935.1"
                     /translation="MTRYSLLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEDLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9215..10024
                     /locus_tag="VYO12_001331"
     CDS             9215..10024
                     /locus_tag="VYO12_001331"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1587936.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGGFKGLLKAILAEL"
     gene            10500..10895
                     /locus_tag="VYO12_001332"
     CDS             10500..10895
                     /locus_tag="VYO12_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV1587937.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10905..11480
                     /locus_tag="VYO12_001333"
     CDS             10905..11480
                     /locus_tag="VYO12_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1587938.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11552..12016
                     /locus_tag="VYO12_001334"
     CDS             11552..12016
                     /locus_tag="VYO12_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1587939.1"
                     /translation="MELEGYNALSLAEVEVFRFIATNAETATQVSKPVQPISQTPVKD
                     KTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGCNQTAGFVLNLPIKENM
                     RNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTLNSKVSDDDVL"
     gene            12674..13384
                     /gene="deoD"
                     /locus_tag="VYO12_001335"
     CDS             12674..13384
                     /gene="deoD"
                     /locus_tag="VYO12_001335"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1587940.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13407..13640)
                     /locus_tag="VYO12_001336"
                     /pseudo
     CDS             complement(13407..13640)
                     /locus_tag="VYO12_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
BASE COUNT         4064 a         2528 c         3096 g         4064 t
ORIGIN      
        1 agattcagtc tactatatat cataatggac aaagggcaag ctgtcaagaa attacagtta
       61 ttttagtaaa aaaagaagca gaaaaatttc attttctact ccttttctat aggatttatt
      121 tttctttttc agaactttta acttcagctt ttttggcaga tttaatctga atgtttccct
      181 tgctagtcat aactgctttg agatcttttt cgcttggatt ttcaaggtag tagtcagtga
      241 ttgtgtcctc aatatagtct tgaatagtcc gacgaagtgg gcgtgctccc atttttggat
      301 cataacctag gtcaaccaac ttttccttga ccttatcagt tacatccaaa cgaatgttgt
      361 tgctagagag gcgcttgtta acatctgcta gcatgagctc gacaatctga aggaggttat
      421 ccttgctgag agccttaaat tcgataatgc catcaaaacg gttcataaac tctgggctaa
      481 agaagttacc gagttcaccg aggacagaat tggtacgtcc ttctctagca gcaccaaatc
      541 caacgctagc ttcggtcttt cctgtacctg catttgaggt catgataatg atggcatcct
      601 tgaagctaac ggtgcgtcct tgcccgtctg tcaaacgacc atcgtccaag acttgaagaa
      661 acatgtgcat aacatctggg tgagcttttt ccacttcatc gagaaggatg agagaatatg
      721 gattgtggcg aactttttca gttaattgac cagcctcatc atagccaaca taacctggag
      781 gggcgccgac caacttagcc acactatgtt tttccatgta ttcactcata tcaaagcgaa
      841 tcatactatc agcagaacca aaaagttcga tagccagttg tttggaaagt tctgtcttac
      901 cgacaccagt tggcccaacg aagaggaagc ttccgattgg gcggttaggg gtaccaagtc
      961 cgacacgatt acggcgaata gccttggcaa tcttatcgac tgcatcatct tggccaataa
     1021 catgagactt gagatcttcg gctagatgga tgagttgaga ttgttctttc tctttcaaat
     1081 caccaacagg gatattggtt ttctgctcga taatgtgctc aatagttttc tcgctgatga
     1141 taggagtatc ctggtctgtg atctttttct tttgcatttc cttatacttg gcaatctggt
     1201 cgcggaagta ggccgccttc tcaaaatctt cttctcgtgt agcttgagac ttgagatttt
     1261 cagcctcaat caagcgctga tcaattactt taggatccac aaaattcaag gtcaagttca
     1321 tcttagaacc agcttcatct aggaggtcaa tggccttgtc aggcaagaag cgatcttgga
     1381 tgtagcgatt ggaaagagtt gcagctgctt caatcgcagc atcggtatat tgaacgtggt
     1441 ggtaatcttc gtatttcttt tgaatccctt tgagaatagt gattgtttcg tccaccgttg
     1501 gttcatcgac tttaacaggc tgcatacgac gctcgagggc agcatccttt tcaatgatac
     1561 ggtattcatt gagggtagta gcaccgacta gttgcagttc tccacgagca agggctggct
     1621 tgaggatatt tcctgcgtcc atattaccat cactcgcaga accagcacca acaatttcat
     1681 ggatttcatc gataaagagg atgatgtctt cacgtttgcg aatttcttcc atgagttttt
     1741 gcatgcgttc ttcaaattgt cctcgaatcc ccgttccttg aactaagcta accacatcca
     1801 gacggatgac ttgtttacct tggagtttat gtggcacatc gccatcgaca attttctgag
     1861 ctagaccttc gacaacggcc gtttttccga caccaggttc accgataagg acaggattat
     1921 tcttggttct acgattgaga atctcgatga cacggataat ctcatcgtcg cgcccaataa
     1981 cggggtcaat gtctccacga cgggcaattt cagttacatt aataccaaat tcttccagca
     2041 ggcctttttc ttggctaggt ggcggagttt gagcagatcc acgattttgg gaaccataac
     2101 cgccgtttcc accgtaacct ccacctgatt gggttggggg aataggagga gtattgctag
     2161 aaggtctgaa attgtttaga tcattgaaga aatcaccaaa gggatcgaag tcacgattgt
     2221 tcagatccgt catacctttg aagaggctat tgttaggatc tgttttgata atcttatagc
     2281 agttttgaca gaggtcaatt tgtttttgtt ttccattgag attggtgtaa agatgaattg
     2341 ttgagtcgtt aattttacag ttttgacaaa gcatacatct acctcatttc tttctttagc
     2401 ctgacttgtt taaaggtcaa aaatagtcaa attctatact ctcattataa caggattggg
     2461 gtgtaaagca agtaaaaaga ttgaagcttt gagaagttgt tacaatgaaa atttttgtga
     2521 atttggacta taaaaaaatc ctatttgtga gacaaaaagg attttactta gacatgcagg
     2581 gacaatccca gcttgtttta gattagtaga gaagttcgta aatttccatt gcgattaagt
     2641 caatgctatc aaacgtatac gtttcgcgat cttctacgtc acgaagggta aagattgggc
     2701 cgttttcttc gtcgtggctg tacgcaactt ctgcaacaac aactccttcg cgttcaaaat
     2761 tacgttttaa atttccgcca tcgtttgcca ttgcttcaag gcggttgatg attctaacca
     2821 aatgtgattc cattttgatt ctccttcatt tcttatcgtt actattttac cataaaggag
     2881 gccaacttga ctagaaaaaa ctaagatttc tcactaattc tgctagttta aagattttta
     2941 aataaattgg ataaaagatt taaattttaa ttcaaaacat gttataatca tacagtattc
     3001 tattttagaa agcagtgtga ctatgaattt ttctttttta cctaagtatt taccttattt
     3061 taactatggg gctgttgtga cgattcttat ttctatctgt gttatctttt tgggaactat
     3121 tttgggtgtt gtcttggctt ttgggcaacg ttcaaagttt aaaccgcttg tttggttggc
     3181 caacttgtac gtttggattt tccgtgggac accgatgatg gttcaaatta tgattgcctt
     3241 tgctcttatg catatcaatg ctccgactat tcagattgga attttaggtg ttgatttttc
     3301 gcgtctgatt ccagggattt tgattatctc tatgaatagt ggtgcttatg tttcggagac
     3361 tgttcgtgcc ggaatcaatg cggttccaaa aggtcagcta gaggcggctt attcgctagg
     3421 gattcgtcct aaaaatgcga tgcgttatgt gattttgcca caagcagtca aaaatatctt
     3481 gccagcattg gggaacgaat ttatcaccat tatcaaggac agctccctct tatcagctat
     3541 tggggtcatg gagttgtgga atggggctac aacagtttct acaacaacct atctaccttt
     3601 aacaccactt ttatttgcag cattttacta cttgattatg acctctattc tgacagtagc
     3661 cttgaaagct tttgaaaaac atatgggaca aggagataag aaataatgac agaaaccttg
     3721 ataaaaattg aaaatttaca taaatctttt ggaaagaatg aagtattgaa gggcatcaac
     3781 ctcgagatta aaagaggaga agttgtcgtt atcatcggtc cttcagggag tgggaaatct
     3841 accttgcttc gctctatgaa tttgttggag gaagcaacca aggggaagct tatctttgag
     3901 ggagtcgata ttacggacaa gaagaatgac ctgtttgcca tgcgtgagaa gatgggcatg
     3961 gttttccaac aatttaacct ctttcctaat atgactgtga tggaaaatat taccttgtca
     4021 cctatcaaga ctaaaggtga aagcagggaa gttgctgaga agagagccca agagcttttg
     4081 gaaaaagttg gtttgccaga taaggcagac gcttatccac agagtctgtc gggtgggcaa
     4141 caacaacgga ttgccatcgc gcgtgggttg gctatggaac cagatgtttt gctctttgac
     4201 gaaccaactt cagccttgga tcctgaaatg gtaggtgagg tattggctgt tatgcaagac
     4261 cttgccaagt cagggatgac tatggttatc gtaacacatg agatgggatt tgcccgtgag
     4321 gtggcagatc gtgttatctt tatggcagac ggtgtggttg ttgaagatgg gacacctgag
     4381 cagatttttg aacaaaccca agaacaacgg actaaagatt tcttgagtaa ggttttataa
     4441 tctattttaa aatttcaaga caagattagt gaaaagttca accagagctt tttcttatag
     4501 tttgaaacta taggataacc taggaaagaa gtgttagagg gcactagcag tttttggtat
     4561 aatggaagat atttgaaaga aaagagaagt gatatgacac agattattga tgggaaagct
     4621 ttagcggcca aattgcaggg gcagttggct gaaaagactg caaaattaaa ggaagaaaca
     4681 ggtctagtgc ctggtttggt agtgattttg gttggggata atccagccag ccaagtctac
     4741 gttcgcaaca aggagaggtc agctcttgcg gctggttccc gtagcgaagt agtgcgagtt
     4801 ccagagacca ttactcaaga ggaattgtta gacctgattg ctaaatataa tcaggatcca
     4861 gcttggcatg ggattttggt ccagttgcca ttaccaaaac atatcgatga agaggcggtt
     4921 ttattagcca tggatccaga aaaggatgtg gatggtttcc atcccctaaa catgggacgt
     4981 ctttggtctg gacatccagt catgattccc tcgacacctg caggaattat ggaaatgttt
     5041 catgaatatg ggattgactt ggaaggtaaa aatgcggtcg tcatcggtcg ttcaaatatc
     5101 gttggaaaac ctatggctca gcttcttttg gctaaaaatg cgacagtaac cttggcccac
     5161 tcacgaactc ataatcttgc caaggtggct gctaaagcag atattcttgt agtcgcaatc
     5221 ggccgcgcca agtttgtgac tgctgacttt gtcaaaccag gtgcggttgt cattgacgtt
     5281 gggatgaatc gagatgaaaa tgggaagctc tgtggagatg ttgattatga ggcagttgca
     5341 ccacttgcta gccatatcac gccagtccct ggaggtgtcg gtcctatgac cattaccatg
     5401 ctgatggagc aaacttatca agcagcactt cggacattgg atagaaaata agagaaacat
     5461 tcgattaata gagtgtattt tctatagcta tatctaaaat ggcagaaatg aatattaaat
     5521 tttagatata agattacaaa aggaggtctg cgtctccttt ttattgtata ataaaagtga
     5581 gaggaaaaaa ggatgaaagt gattaatcaa accttattag aaaaagttat tattgaacgt
     5641 tctcgttcaa gtcataaagg agattatggt cgtctgctgc tgcttggtgg tacttatcct
     5701 tatggaggtg ccatcatcat ggctgcttta gcagctgtaa aaagcggtgc aggattggtg
     5761 accgttggaa cggacaggga aaatatcccg gctctgcaca gccatttacc tgagcctatg
     5821 gccttttctc ttcaagacca gcaattgtta aaagagcaat tggagaaggc agaagttgtc
     5881 ttgctggggc ctggtttacg agacgatgct tctggagaaa atctagtaaa acaggtcttt
     5941 gttaatttaa gccaaaatca gattttgatt gtagacggag gtgccttgac cattcttgct
     6001 aggacaagtt tgtcatttcc ctcaagccag cttatcctaa ctcctcacca aaaagaatgg
     6061 gaaaaattat ctggaattac gattgaaaag caaaaggaag atgcaacggc tagtgttctg
     6121 acttccttcc ctcaaggaac aattttggtg gagaaaggcc cagctactcg tatttgggaa
     6181 gttggccaat ctgattatta ccagttacag gttggtggtc cttatcaggc gactggggga
     6241 atgggagata ctctggctgg gatgatcgca ggatttgtag gccaattccg acaagccagt
     6301 ctctacgagc gtgtggcagt agcgacccat cttcattcag ccatagccca agaactatct
     6361 caagaaaatt atgtggtctt accgacggaa atcagcaggt atcttcccaa aataatgaaa
     6421 attatttgtc aacaagagag ggtaagtaaa gataaacttg tttaaaatag acagataaat
     6481 ctacttcctt ctcttaattt atgattcttg aaatgcgcct gctttaaggc taaactagac
     6541 gtatcaaagt taagattttg tttttgtaac tggaaagaga tgaacaaatc aaatacataa
     6601 aagataaact catttttatt cgtttggtaa aaataaaatg catattttta aagaaagatt
     6661 gatgaaaaat gtagatttga tgtttttact tgaataaaaa gcggtttcat agtaaaataa
     6721 gaactgagaa tgatggaggt aaatcggtgg aaaatctgaa gaaaatggca ggtatcaagg
     6781 ctgctgagtt cgtgagcgat ggaatggtcg ttggacttgg aacaggctcg actgcctatt
     6841 attttgtcga agaaatcggt cgtcgtatca aggaagaagg attgcagatt acagctgtaa
     6901 caacttctag tgtgaccact aaacaggcag aaggccttaa tatcccgctc aagtctattg
     6961 accaagtaga ctttgtcgat gtgacggttg atggcgcgga tgaagtggat agtcagttta
     7021 atggaatcaa aggcggtggt ggtgcccttc tcatggaaaa ggtggtcgca acaccatcaa
     7081 aagaatacat ttgggtggtg gatgaaagca agctggtcga aaaactaggt gcttttaaat
     7141 tgccagtaga agtggttcag tatggtgcag agcaggtctt tcgtcatttt gaacgagctg
     7201 gctacaaacc aagtttccgt gaaaaagacg gccaacgttt tgtgacggat atgcagaatt
     7261 ttatcattga cctcgccttg gatgtcattg aaaatccaat tgcttttgga caagaattgg
     7321 accatgtcgt tggtgttgtg gagcatggtt tattcaacca aatggtggat aaggtaatcg
     7381 ttgctggacg agatggagtt cagatttcaa cttcaaaaaa aggaaaatag aagggggcat
     7441 aagatgtcta aatttaatcg tattcatttg gtggtactgg attctgtagg aatcggtgca
     7501 gcaccagatg ctaataactt tgtcaatgca ggggttccag atggagcttc tgacacactg
     7561 ggacacattt caaaaacagt tggtttgaat gtcccaaaca tggctaaaat aggtcttgga
     7621 aatattcctc gtgaaactcc tcttaagact gtagcagctg aaagcaatcc aactggatat
     7681 gcaacaaaat tagaggaagt atctcttggt aaggatacta tgactggaca ctgggaaatc
     7741 atgggactca acattactga gcctttcgat actttctgga acggattccc agaagaaatc
     7801 ctgacaaaaa tcgaagaatt ctcaggacgc aaggttattc gtgaagccaa caaaccttat
     7861 tcaggaacgg ctgttatcga tgattttgga ccacgtcaga tggaaactgg agagttgatt
     7921 atctatactt cagctgaccc tgttttgcag attgctgccc acgaagacat tattcctttg
     7981 gatgaattgt accgtatctg tgaatacgct cgttcgatta cccttgagcg tcctgccctt
     8041 cttggtcgca tcattgctcg cccttatgta ggtgaaccag gtaacttcac tcgtacggca
     8101 aaccgtcgtg acttggctgt atctccattt tccccaactg ttttggataa attgaatgag
     8161 gctggtatcg atacttatgc tgtgggtaaa atcaacgata tctttaacgg tgctggtatc
     8221 aaccatgaca tgggtcacaa caagtcaaat agtcatggaa ttgatacact attgaagact
     8281 atgggacttg ctgagtttga aaaaggattc tcattcacaa acctagttga ctttgatgcc
     8341 ctttacggcc atcgtcgtaa tgctcacggt taccgtgatt gcttgcatga gtttgatgaa
     8401 cgcttacctg aaattatcgc agctatgaga gagaatgacc ttctcttgat tactgcggac
     8461 catggaaatg acccaacgta tgcaggaacg gatcacactc gggaatatat tccattgttg
     8521 gcctatagcc ctgcctttaa aggaaatggt ctcattccag taggacattt tgcagatatt
     8581 tcagcgactg ttgccgataa ctttggtgtg gaaactgcta tgattgggga aagtttctta
     8641 gataaattgg tataagatga cgcgctattc tttgctggtg agaggtatca atgttggtgg
     8701 taagaataag gtcgtcatgg cggagcttcg tcaagaattg acaaacttgg gactggaaaa
     8761 ggttgagagc tacatcaaca gtggcaatat tttctttact tcgatagatt ccaaagccca
     8821 attggttgaa aagctagaga ctttctttgc agtccattat ccatttattc agagcttttc
     8881 tttactgagc ctagaggact ttgaggcgga acttgaaaat ctaccagctt ggtggagcag
     8941 agacttggca cgaaaagatt ttctctttta cactgaggat ttggatgtgg accaagtcat
     9001 cgcgacagtt gaaagtttag agctgaaaga tgaagtgctt tattttggaa aacttgggat
     9061 tttctggggg aaattttctg aagaatccta ttctaagact gcctatcata agtacttgct
     9121 gaaggtgcct ttctaccgcc acattactat tcgtaatgct aaaacctttg acaaaattgg
     9181 tcaaatgcta aaaaaataat aaaggagaca cacaatgaca tttttaaaca aaatccatga
     9241 aactgctact ttcctgaaag aaaagggaat tgcagcccct gagttcggtc taatccttgg
     9301 atcaggactt ggagaattgg cagaagaaat cgaaaatcca gttgtagtag actatgctga
     9361 gattccaaac tggggccgtt caacagtagt cggtcatgct ggtaaattgg tatatggtga
     9421 actggcaggt cgcaaggtct tggctcttca agggcgtttc catttctatg aagggaatcc
     9481 tctggaagtg gtgactttcc cagttcgtgt gatgaaagtt cttggatgtg aaggtgttat
     9541 tgtaaccaat gcagctggcg gtatcggatt tggtcctggt accttgatgg ctatctcaga
     9601 ccatatcaac atgacggggc aaaatccatt gatgggtgaa aacttggatg actttggccc
     9661 acgtttccca gatatgtcta gggcttacac accagaatac cgtgccactg cccatgaagt
     9721 ggctaaaaaa cttaatatca agcttgatga aggtgtctat atcggagtta ctggtccgac
     9781 ttatgaaaca ccagcagaaa ttcgttccta taagacactg ggagcagatg cagttggtat
     9841 gtctacggtt cctgaagtta tcgtggcagc ccactctggc ttgaaagttc tgggaatttc
     9901 atgtatcact aactttgcgg ccggtttcca agaagaactc aatcacgaag aagttgtaga
     9961 agtgactgaa cgtgttaaag gtggtttcaa aggcttgctt aaagcgattc ttgctgaatt
    10021 gtaagaaaaa agatttaaaa gggggagtgc ctctgtcttt tcaggattga ctgcctatcc
    10081 gtattaaaga agaaacagag gaatactatg agcttcttcc tgctcttata actgaaagaa
    10141 gcggaagaat aggtatgtct gatctgatag ccagcattgt gaaagacaag attctaggat
    10201 actagcatta gcttcctagc caagcagact agtatgataa ggagagatga gaatgaattg
    10261 actttctgaa tttctcagtc ttatcatata tagcacaatg agatttcgct tgagtctgct
    10321 tgtaaataaa cgaaaagaaa gataagaaat aatgaaaatt ggtcaacgaa ttatgcgctt
    10381 tggcataaaa aattaagtat cggagttgta tctgttgtag tcggctttga tttctagctc
    10441 cagctggaat ttcagccaat gaagtaaagc aagatgtaac atctgaagtg gtaataggtg
    10501 tgctagattc taaggaggaa ttgaaagagt cagaaaatga tgctccaaaa ctagaaactc
    10561 ctcttagaga ggagccaaga ctagctcctc aaacgcttcc ggaagcaagt gaagttcttg
    10621 aaaacaaaag ggaagagtca aaagtagaga taacagagcc agctcaagcg gatgatatcc
    10681 gcaaggttgt tggggaatta gccaaggata taagtattac taagttgtat atgacaggtc
    10741 attctcttgg aggctaccta gctcagattg cagcggttga agattaccaa aaatatcctg
    10801 atttttataa ccatgtattg aggaaagtga caactttcag tgctcctaaa gtgattactt
    10861 ccagaactgt ttgggatgct aagaatggtt tctgagatgt tggtttggaa agtcgtaaat
    10921 tagctgttag tggaaaaatt aagcattatg tggttgataa tgacaatgtt gtgactccct
    10981 tgattcataa taatcgtgat attgttacat ttacaggtaa ttcacgcttt aaacaccgtt
    11041 ctcgtggcta ttttgaaagt ccaatgaatg atattcctaa ctttaatatt ggtaaacaag
    11101 ctaccttgga taaacatggt tatcgtgatc cgaaattgga taaagtgcga ttctttaaga
    11161 aacaggctct acctcaatct tctagtcaac caagcgctga accaatggaa aatattgcct
    11221 taggaaaaca ggttactcaa agttcgacag ctttcggagg agatgctaga agagctgtgg
    11281 atggcaaagt cgatggtaac tatggtcaca attctgtcac tcatacaaac ttccaatcta
    11341 agccttggtg gcaagtagat ttggctaaag aagaaaccat tcgccaaatc aatatttaca
    11401 accgaacaga cactgcccag gatagattgg caaactttga tgtcattctt ttagacagtt
    11461 ctggtaaaga aattgagtga aaacgtataa tatctcctaa agatgtgtca gcacaaatta
    11521 cgattaacca taaaaaacgc gctatgttcg gattgagcta gaaggctata atgccctcag
    11581 tcttgcagaa gttgaagttt tccgctttat agctacgaat gctgaaacgg cgacacaagt
    11641 ttctaagcca gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat tgacaattca
    11701 acacagtgga gcttacattg cccgctactc cataacttgg gaagaagttc cagtagataa
    11761 agatggaaac caagttgttc gtagtcattc ttgggaagga aacggttgca accagactgc
    11821 aggttttgtc ctcaacctcc caatcaaaga aaatatgaga aatctgcgag ttaagattga
    11881 gaaaaagacg ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttt
    11941 agctcaaccc caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga
    12001 cgatgatgtc ttgtaatctg atggtacaat gacagttagt ttgtctagtt tataagaaag
    12061 tactacctga gcttgaatag aactcaggta gctctctgtg aaagaacaaa attaatactc
    12121 aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt tgctcaaagc actgctttga
    12181 ggttgtagat aagactgacg aagtcagtca catatataat ccaaggcgac gttgacgtgg
    12241 tttgaagaga ttttcgaaga gtataaacag aaaggtagag cgcgtgttct aatttgaaca
    12301 cgagtagaaa acttttctaa aaacaaaaac gaaaggatgg gtaaactgta ttcgctgaac
    12361 tgaatacggg cgactctcct ctaaatcaaa attaagaaag aaaggaattg accccaccct
    12421 aaaagcagtg ggaaaaagat agttggtcta gcgagcatcg ctcactgcgc ccaactccta
    12481 ttttcccttc gctttttgac gggtttggta tctttctcaa tataaaatat aaaataaaga
    12541 aaggtagagc gtgtgttttg atttgaacac gagcggaaaa ctcggaaaat agataatctg
    12601 actgaaaaat caggatttct cgtcggcttc ctaattttca gtcgttttct tctcgctctt
    12661 tgtatcataa attatgtcta tccatattgc tgctcagcag ggtgaaattg ctgataaaat
    12721 tcttcttcct ggggatcctc ttcgtgctaa gtttattgcg gagaatttcc ttggtgatgc
    12781 tgtttgtttt aacgaagtgc gtaacatgtt tggttacact ggtacttaca agggtcaccg
    12841 tgtatctgtc atgggaactg ggatgggaat gccatctatt tcgatttatg cgcgtgagtt
    12901 aatcgtagac tacggtgtga agaaattgat tcgtgtggga actgcaggtt ctttgaatga
    12961 agaggttcat gttcgtgaat tagttttggc gcaggcggct gcaaccaact caaacatcgt
    13021 tcgtaatgac tggccacagt acgattttcc acaaattgct agctttgatt tgcttgataa
    13081 agcctaccat atcgccaaag aacttggtat gactactcac gttgggaacg ttttgtcatc
    13141 tgatgtcttt tactcaaatt actttgaaaa gaatatcgag cttggtaaat ggggagtcaa
    13201 ggctgtggaa atggaagcag cagctcttta ctatcttgct gcccaatacc atgttgatgc
    13261 gctagctatc atgaccatct ctgatagctt ggtcaatcca gacgaagaca caactgcaga
    13321 agaacgtcaa aataccttca ctgatatgat gaaggttggt ttggaaacct tgattgcaga
    13381 ataattatag ccaaaaagga agccctttat gaattgtagg acttcctttt cttatccaga
    13441 aattgatcta gctctctctg atttcgaaga atagtgactt tatgtgaata ttcttggcaa
    13501 agtttttggt aattttcttt ttgagttttg ctacgtccat cccaaagaat ccatctgata
    13561 aactcccagt caaagcgttc agggcaatct gccgccatac tttctctgac ttttccacgg
    13621 tatttaagat aacgcttaaa gggctctata atatttgtag tgggtaaatc ccttatagat
    13681 attatggagc ctatttttgt gtagaaaaaa agtcccatat gacctataat gaaaagcgac
    13741 aaaacaactc at
//
LOCUS       DAVLXF010000047        13710 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLXF010000047 DAVLXF010000000
VERSION     DAVLXF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.047"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(771..1266)
                     /locus_tag="VYO12_001337"
                     /pseudo
     CDS             complement(771..1266)
                     /locus_tag="VYO12_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1244..1582)
                     /locus_tag="VYO12_001338"
     CDS             complement(1244..1582)
                     /locus_tag="VYO12_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1587941.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            2048..2911
                     /locus_tag="VYO12_001339"
     CDS             2048..2911
                     /locus_tag="VYO12_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1587942.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            complement(3097..3567)
                     /locus_tag="VYO12_001340"
     CDS             complement(3097..3567)
                     /locus_tag="VYO12_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV1587943.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(3533..4339)
                     /locus_tag="VYO12_001341"
     CDS             complement(3533..4339)
                     /locus_tag="VYO12_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1587944.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(4546..5187)
                     /locus_tag="VYO12_001342"
     CDS             complement(4546..5187)
                     /locus_tag="VYO12_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1587945.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(5184..7292)
                     /locus_tag="VYO12_001343"
     CDS             complement(5184..7292)
                     /locus_tag="VYO12_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1587946.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(7344..7640)
                     /locus_tag="VYO12_001344"
     CDS             complement(7344..7640)
                     /locus_tag="VYO12_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1587947.1"
                     /translation="MIGEINHEKISVASLMVCGILATTNALAVWVEGGQWNYGVGWTG
                     TFGYSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            8045..8365
                     /locus_tag="VYO12_001345"
     CDS             8045..8365
                     /locus_tag="VYO12_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1587948.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(8417..9004)
                     /locus_tag="VYO12_001346"
     CDS             complement(8417..9004)
                     /locus_tag="VYO12_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1587949.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQKLVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            9246..9917
                     /gene="sdaAB"
                     /locus_tag="VYO12_001347"
     CDS             9246..9917
                     /gene="sdaAB"
                     /locus_tag="VYO12_001347"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1587950.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            9926..10798
                     /gene="sdaAA"
                     /locus_tag="VYO12_001348"
     CDS             9926..10798
                     /gene="sdaAA"
                     /locus_tag="VYO12_001348"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1587951.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            10820..11452
                     /locus_tag="VYO12_001349"
     CDS             10820..11452
                     /locus_tag="VYO12_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1587952.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            11502..11759
                     /locus_tag="VYO12_001350"
     CDS             11502..11759
                     /locus_tag="VYO12_001350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587953.1"
                     /translation="MKIKEQTRKLAAGCSKHCFKIVDGTDEVSSKHCFKVVDGTDEVS
                     SKHCFEVADRTDEVSSKHCFKVVDGTDEVSNHIRQGDVDVI"
     gene            complement(11845..13695)
                     /locus_tag="VYO12_001351"
     CDS             complement(11845..13695)
                     /locus_tag="VYO12_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1587954.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
BASE COUNT         4291 a         2966 c         2351 g         4102 t
ORIGIN      
        1 aaagtcaata tatctcactc ttttcaacga caagaaagag gctgataatc gaccaacctc
       61 tgtttttaga tacagttcaa gtccaaacta ttctttatag aaaagtccga taaattcgag
      121 caatagttaa catacttaca aatataactc ccaaaataga taacactatt ttcacaaaac
      181 tcagttccga acctatgaca tatatatgaa aaagaagggc tagtcctaca catatataca
      241 ataagtagcc aacaagtttt atctcaccaa ttcctgcaaa aaaatgtttc atgtgacgat
      301 tctccattta ctccattaaa tcagttttat tttagactat cataacacaa tcacctcttc
      361 aataaaaacc atttgcgaaa tactattctg aagcattgat agctcagatt tctcagaata
      421 gagctgtcct aactatcgag cagctcaaat acatatatat gagcaattct aagcctagaa
      481 ttgctataaa ataaatgttt ccaggctatt ctaagtcttt gactgcttaa aattttacat
      541 tctgaaggat tctaaagcta gaacagctca aaatcacgag aactaaggta ttcgaaactt
      601 cagagtaata caatccccat aggaattctc cattcttatt aagcatgatt aaaatgtaat
      661 cctaacaagt gatcctcgac tgtgtttgta gcacttagtt acctagataa gactatttcc
      721 ctttatcata tctgatttta agccgttcta agatttaacc tatgtttcta ttacttgcta
      781 tttttaaata attcgtattc ctttgctttg ataaatggaa ctgaaaaatt agaaaaaaga
      841 agtaaaaccg aaaacaaata gcctgctttt tgaatcacta aaagatatcc taaaaataag
      901 taagctataa catccaatgc gaaaagaaaa aatattctcc tcttcattaa agtacttgtg
      961 tcaaataatc tgatgaataa aaattttatc gtagtatcta cacaaatttt tatcaaaaag
     1021 ataagagcca atacacctgc tgctgcacca ataagccaac ctccaaggcc tccaacctta
     1081 atatagttta ttagtttttt tgttaaaact gcagctccag tcaatgctat aaaagttcct
     1141 gggctcatag ctaaagctgc gcaaattcct tgacaagcac tcttgcttaa ataagccccc
     1201 cccatcaaga tacatcatct cctcttgctc aagagcaaca taattatttg gtaatacgag
     1261 ttccatttat tttctccttt atactatgta ataccacaaa atgattttga cctaaatcat
     1321 tcttaacttg aagaatcgca ggaaatgtta attttggagt caaatcttcc agtgccactc
     1381 gaacagcttt tactgtaaaa ttcaacttat tcagacctga aacaatacca ctgacagtcg
     1441 ttccatacat gtctgtacca ataatttccc gaatcttcat gattgatagt tcctttttgt
     1501 aagataaaag aactgttgaa acaactgccg cagcacaatc tgactgatca tgttgcatcg
     1561 tacaaggata tttttttaac atagaaatac tcaattcttt agaattaatc taacttttct
     1621 ttgatgtcac ccaaacctca atcactgccc agtagttctt gaatcagttt attttcaatc
     1681 gtatcattct ttgttcttga tatctttcca agcattgtag tacagcagat ttttgtccaa
     1741 ggactgagcc tcctttaaca actcaagctc ccttacatta tatcgaccag ccccatggaa
     1801 agattatgac cagactaatc aatgatatca tacatactag aatacaaaat atttttttat
     1861 tcatactttt tctcctatct ttattttcta cctacatcat acctcttatt aagctcattt
     1921 taaaattacg ttaacttttt tcacaaattt atttcgactt tcttttttac tttttacatt
     1981 gctatgatac aatattttta acaagatttt attatttgtt aacttttttc acagaaagga
     2041 gaaaatcatg cgttatgatt tcggaaaggt ctataaagaa atacgtgagt caaaggggct
     2101 gacccaagaa gaggtctgtg ggggtgttct ctcaagaacc agcttatcaa aaatcgaaag
     2161 tggcaagaca accccaaaat atgaaaatat ggaatttctt ctccgtcaaa tcaatatgag
     2221 ttttgaagag tttgaatata tctgtcaact ttatcaacca agtcaacgca cagagattat
     2281 gcaaacctat ctcaatatgc gttccattat cgggaccagc gatcttgtca atttatttca
     2341 aaaatgccaa gactacctca agacccacca cgatctccct atagaagaaa tcagagatat
     2401 gcttgaagtt gtcatttata tccgtcaaca cggggcaggg gaactatcag atcacgcaga
     2461 acaggttgtc aaaaaacttt ggagaaaaat tgaaaaacaa gatacatggt atgagagtga
     2521 tctaaaaatc ctcaatacca ttcttttcag ttttcccatt gaatacctcc atctcatcac
     2581 tggaaaaata ttgcaacgcc tagaagtcta taaaaactat caacatttat atgacttgcg
     2641 aatagcaatc ctgctcaacc tttccacact ctatctatac aatcaagata aaaacatgtg
     2701 taagcaaatc tgctacactt tactggagga cgccaagaat aagaaaagct acgataggct
     2761 ggctatctgc tatgtccgca ttgggatttg tacagacgat tctaaactta tccaaaaagg
     2821 tttctcactc ctagagctga ccgaggaaac ttctatgctg tctcatctca aaaaagaagt
     2881 agagatctat taccaagcga aggaaagata aaaaagccga gggagctcct cgaccttttc
     2941 atagaatcgc cgaacgattt aacgagaaag tatgactttt acgtttatcc caactcaatt
     3001 atgacatttt tttcaaaagt caatatatct cactttttca acgacaagaa agaggctgat
     3061 aatctaccaa cctcttattc tgaacccatc actccatcac tttttagctt cattcgcttt
     3121 cttagcgact gcaatctggt attcgacttg gtcattcccc ttaccggtac aaccatgagc
     3181 aattgtagtc gctcctatct gatgcgctat ttcaaccaat tttttagaaa tcagagggcg
     3241 gctcaaggca gataccaagg gatacttttg ttcataatag gcatgtgact gaagagccac
     3301 tagcacataa tctgtagcaa attcgtcctt aacatcaatg acataagatt ctactgcccc
     3361 aaccttaaga gccttatcat ggatgaaatc taagtctttc ccttcaccca catccataca
     3421 aactgaaaca acatcatagt ctttctttaa ccaggtaata gcaactgatg tgtccaaacc
     3481 gccagaatag gccaaaatta ctttttcttt actcatgttc ttttccttct ttctattttt
     3541 caatggatgc tttaaaggca tcctcaatga gtttgtctaa ttccccagac tccttcaact
     3601 tttgaatggt tttatcgact gcctctttca attccttgct atcttttttc atggctaccg
     3661 cgtaggaatc atcttgctct ttttcaaaat tgaggtctgc gattgctaaa tcaggattat
     3721 tttccacaaa tcccttggaa acaggttctt caaagataac ggcatccact tgtcctgatt
     3781 ttaaatctgt gattaaattc ccatttttag gcagagatac gagggaagaa ttttgtagca
     3841 aatctttcgc catcgtctct tgaatcgaac ctttctgcgc tccaaccttt ttctgcgcca
     3901 agtcgtttac agactgataa gtagtcaagt cagatttttt gacaatgagt ttattttttg
     3961 cagtatagta gggaatggaa aagtcaaaca ccttgctccg ttcatctgtc ttagaaacac
     4021 ctgatatggc aaggtcggct tttcctgatt gaacactagc cagtacatta tcaaaactca
     4081 tgggagatag ttccaattcg acacctagtt ctgttgcgat agccttggct aattcgatat
     4141 ctgaacccac aatctgattt ttcccatcaa ccactttttg gtattcaaat ggagcaaaat
     4201 ccggattgag ggctacaacc aattttcctt tggacttgat ggcttcaatt ccagccgatt
     4261 gattgttgct acaagcaaat agtaggggga gcatcactaa accaaacatc gtcatcaaca
     4321 ccttcttcat cttattcata ataaagccta ctttatgttt attccttttg tttgtataaa
     4381 caatacatct atattcatcc tttgtcaagc ctaaattgaa aaaattttca atttgaaaca
     4441 aaaaaaacct agaaatacta cataaaatgt cattcctagg cgtatttatg ctatttttct
     4501 ctggaaaaca tgagtattca gccagtcaaa aggagctgaa tgaactcatt ttccctcgcc
     4561 caattcaatg acacgatgac attgttgggc tacataagca tcgtgggtca caataatgac
     4621 tgttttccct tctcgattca tctctaagag aaacttcaag accaaatctc tattttcagg
     4681 atccagcgaa cctgtaggtt catcggctaa aatcagctgg ctgggtttta agatggctct
     4741 agcaactgca attcgttgtt gttcgccacc agacaactcg gagacccttt gatgcaaagt
     4801 agctgacaaa cctactctct ctaaaatctc ttccaccttt ttgagcttgt ctttcttagg
     4861 caatttcaca tatttcagcg ccagcatgag attgtactcg accgtttcat catcaatcag
     4921 ggcaaaattt tgaaacagat aagagatatg ttcacggatt attgtttgcg acttagcaga
     4981 attaaccgct agatttgtct gaccaaaaat ctcataccgt ccgctataat caccatctat
     5041 caaacccaat aaatttaaca aggtcgactt cccactacca ctcttaccaa caatagctac
     5101 caaatccccc tgatcaatcc tgagagataa gttatccaaa atcacttttc ccccaatggt
     5161 tttggtaata tttttcaact caatcataag atgccccctt tcaataactc tactagactt
     5221 cttttctcca tcctagaagc taagcctagc acaaatagta tatccagaca tgtaaaacct
     5281 gcaaacagta gaagtggtaa gaacgcatgg gcaaagaaaa tcaagactag aagagggaaa
     5341 ctatagccca gcaagagcag aacgaggaga ggacggtagc gatcgaccag tttccacccc
     5401 ataaacttct tggtaatgat atccctgcgc ttcaataaga aagttgttac tagtaagaag
     5461 taggaaatca tcatgctaag gagaccaaac aaagcaaaga gtaggttaaa attccgaaca
     5521 gcatctctat aagaatccac tttctcttgt tgaatggctt gaatagatga aaattttaaa
     5581 taatttccat ctgacaattt ctcaactaac tctgtaatct ctttttgatg ttgaaccgta
     5641 ttttcaattt taatcggatt atttaagcca gttgttgaca gggaggcttt ctcatcccac
     5701 atcatatcag aatcattgac caagctaata attggattgg agagattttc ctttcgctta
     5761 tcactatatg ggaaaaatga ccaatctcct tcataatagg caatctcgac atccatctcc
     5821 tctatcgttc gtttttgctg ctcttcatac ttcatcgaat gaaaggcaat taacttcccc
     5881 aagagctgat ttttatcttc ttcacctttc gtacttgctg gcatcaaaat aactttttta
     5941 ataccggtat ctggtagctt gaatcccttg ctctttagaa aattgcgatt ggcatagtaa
     6001 acatccaccg tatctgttaa ctgatattgc tgaatctgtt ctgattggac aaaatttttt
     6061 acaggaagac tgctactctg cacatagccc gcctgcgttt tttctaccaa atcctgataa
     6121 aatcgataga aataatctgt agatttccct gaccctgcta gctcttcttg ccacagatta
     6181 tcattgagtt tgaaggtttc taaggtcagg taattacctt gacttaccca ctgttgctga
     6241 taagcaagtt ctttgttttc ttgttctaaa cttctgccca ccccaatcag taaggccgtc
     6301 agtaaaatag ttgtccctat tttcatcaca taattgaaga taagaccaaa tttgaaagat
     6361 gaaaaacctt tcagcagaga gctgattgtc attttttgga ttaagaggta agtcaaccaa
     6421 ctgataaaga gataaagctg caacagcaaa aaatgagaca accacagcat aggaaacaaa
     6481 tcttttggct tataatcaag caagaaaaac acgcctagat tgatcacaag agccccacct
     6541 aggaggaggt aaaggttgcc ttttacaaca tcagctaaaa cagccctatc ttgaaaacca
     6601 agtaattttt gtaccccaac tcttttcatc tccatcatcg gttgatacac tgtcactaac
     6661 acaagaagca aaatagccaa gacaaaaaca atggcagata aaagcaaatc tcgatttatg
     6721 acttccactg cacttttgta ggtcggctct agcaaggtag cctggtctat cttgaaaaaa
     6781 tcgctccatt tctgtacaat cctatccttg tccatctctt gtgtagaagt tatcgtatag
     6841 cgaccattta aactacgaga tgtatccttg atataggttt gaaaagtcat aagctgaata
     6901 ggtttggctt ttagaaaggt cggaatcgta ccaagtttat tggaaatttc tttattacta
     6961 tagactcctt caccatctgt ggtaaaatca agagaagaaa tcccaaactc ttggtagggg
     7021 aaggtatctt tatcaaaaac accagacttg accacctcat caccactgtc tgttttgatg
     7081 atggagactt tatactcctt tgatacatcc tcaaaaaatc gaagaacaga cgctgcaggt
     7141 tcgttaatat ctttcaaata caaatccaaa gaatctacag tctttcccat ttcttgaatc
     7201 cgaaccactt ctcgtgtata gtttacatta aaaacaaaaa aagtggtaca cagaagcagg
     7261 agtaacatac agaaattact gatttttttc ataaaagtgc ctccactaca gttttccctc
     7321 tccaaatagg agagggaaga attttactca aatccataga aatattccat tcctgttggc
     7381 ggaatcttgg tgagggaagc tctagcccat gcctcaggtt ttgcataatc cttagaggtt
     7441 ctacccccat gtctaacagt tgctgtatga tatcgagtag aatgtaagta atcagaatat
     7501 ccaaaagttc ctgtccaacc tactccatag ttccattgac caccctcaac ccatactgct
     7561 aaagcatttg tagtagcaag tataccacat accattaaag atgcaacact aattttctca
     7621 tgatttattt ctcctattat cgagagctgt tccgtaaaaa cagctatagt aatttatcaa
     7681 caagtatcgt agtccattgg actcaccttc cttctatctc atttcatttt taacaacgaa
     7741 cttactctta accgggagaa tggtgcgctt ctgtatcagg tccttgaaca accagagtag
     7801 tattaagcac actctaacta ttactcaaga atcagtccaa taaacaatat ggatagcgct
     7861 ttcaaatttt taaactccta tcccatcctt ttatctataa aataagtgaa aatataataa
     7921 ctgtcaagta actgaagtga attttataaa aaaattacaa gccaaatttg taaagtttac
     7981 actaagccgc tagggcaatc gtctatcaga atatccgttt atttgtcaat aatccgagaa
     8041 aatcttgcaa cgcttagaag tctataaaaa ctatcaacat ttatatgact tgcgaatagc
     8101 aatcctgcta aacctttcca cactctatct atacaatcaa gataaaaaca tgtgtaagca
     8161 aatctgctac actttactgg aggacgccaa gaataagaaa agctacgata ggctggctat
     8221 ctgctatgtc cctattggga tttgtaggga tgatgcgaaa cttatccaaa aaggtttctc
     8281 actcctagag ctaaccgagg aaacttctat gctgtctcat ctcaaaaaag aagtagagat
     8341 ctattaccaa gcgaaggaaa gataaaaaag ccgagggatt tcctcgactc tttacatatt
     8401 atttgttcgt tagttcttaa taccagccat tgttaagcca gaagttttta gcagctgtcc
     8461 atgaaccgta acgtcctgca acgtaggcat ctgctacacg ttcttggttt tcagctgagt
     8521 agtcaccgtt caggtatgaa tctgttaatt ggtaacgtcc gatataacgt ccatttgtag
     8581 ctgtatagct accacctgat tctttttgag cgatccattc tttggcttct gcttcagatc
     8641 cgcttacagt tgaaacaact gtttcactta ctactggagt ttctgccact ggagctgaaa
     8701 cagtttcatc ttgagcggct ggtgccgcat aagtagctgg cgctggtgtt gcaacaggcg
     8761 ctacagggcc atcgataacc aacttttgat caacataaat caaatgaatg ttatcaatgt
     8821 ggttgttttc tgccaatttt tcaactgttg tgttgtgagt ttcagcgatt tctgaaagtg
     8881 tatcaccttc tttaacagtg taagttgatg attcttgagc agatacaaat gatggagcaa
     8941 atactgcaaa caaggcagct actcctgcaa gagttgcttt aatcttttta gttattgatt
     9001 tcatattcga aaattctcct tctttctata gttctatcat accctttaaa tattaccgtt
     9061 tcttgacact tttatgtaga aatattacaa aattattttt tatttcccta aataaggtgt
     9121 tttttgtcat aaaagatact atttttatgc tatttgaggt attagaaagg ttttcatcca
     9181 caattaaagc caagcccctc attctttgat ataatagaga taagaatttt tataagggga
     9241 aactgatgaa atcacttcgt tttcaatctg tctttgatat catcggacca gttatgattg
     9301 gcccatctag tagccatacc gctggtgctg ttcgtattgg gaagattgtc tcttccattt
     9361 ttgatgatac tccgacagaa gttgaattcc aactttttaa ctcatttgcc aagacctatc
     9421 gtggtcacgg aacagaccta gcccttgttg caggtatttt aggaatggat acagatgatc
     9481 ctgaaattcc aaatagtctg gaaattgccc acaagcgtgg tatcaagatt gtctggacca
     9541 ttcagaaaga cagcaatgcc cctcacccta acaccactaa aattactgtt aaaaatgccc
     9601 acaagaccat cagcgtgact ggtatttcta tcggtggagg aaatattcag gtaaccgaac
     9661 tcaatggttt tgccgtctct ctcaatatga atacaccgac tatcatcatc gttcatcaag
     9721 atattccagg tatgattgcc cttgttacag aggcgctttc ccgctatggt atcaatatcg
     9781 cccagatgaa tgtcactcgt gaaaaagctg gtgaaaaagc cattatgatt atcgaagttg
     9841 acagtcgcaa ctgtgatgag gctatcgaag aaattcgaaa aatccctcat ctccacaatg
     9901 tcaatttctt taaataggag gaagcatgtt ttattctatc aaagaattgg tcgagcaagc
     9961 agatctggac tttcaaggaa atgtcgcaga actcatgatt acaacagagt ttgaattgac
    10021 cggtcgcgaa cgtgaagaag tcttccttct catggaacgc aatctggaag ttatgaaagc
    10081 ctctgtccaa cttggcctca atgaaaataa atctcgtagt ggcctgacag gtggagatgc
    10141 tgccaaattg gatcactaca tcgaaaacgg aaaaactctg tcagattata cgattctctc
    10201 ggctgcccga aatgccatcg cagtcaatga acacaatgcc aaaatgggct tggtctgtgc
    10261 cactccaacc gctggaagtg ctggctgtct gccatccgtt ctcactgctg ctattgaaaa
    10321 attagacctc agccacgagc aacaattgga tttcctcttt gctgctggtg cctttggact
    10381 agtcatcgca aacaatgcct ccatctcagg tgctgagggt gggtgtcaag ctgaagttgg
    10441 ttcagcctct gctatgagtg ctgccgcctt gactctggct gcaggtggaa caccttatca
    10501 ggccagtcaa gctattgcct ttgtcattaa aaatatgcta ggcctcatct gtgaccctgt
    10561 tgcaggtttg gtcgaagttc cctgtgtcaa acgtaatgcc atgggagcta gctttgcttt
    10621 catcgcagca gacatggcct tggcaggtat cgaatctaaa atccctgtgg atgaagtgat
    10681 cgatgccatg taccaagtag gagcaagcat gccaactgcc tttcgtgaaa cagctgaagg
    10741 tggactcgct accaccccta ctggtcgtcg cctccaaaaa gagattttcg gagaataagc
    10801 ttatcaaata ggagaaacca tgacctctat cacagcgatt tttttcgatt tggatggaac
    10861 cctcgttgat agttctatcg ggattcacaa tgcctttacc tataccttta aagagctggg
    10921 ggtgcctagc cctgatgcca aaactattcg tggttttatg ggaccacctc tcgaaagtag
    10981 ttttgcgacc tgcctgtcca aagaccaaat ttctgaagcc atgcagatat atcgttctta
    11041 ctataaggca aaaggcatct atgaagctca actctttcct cagattatag acttgcttga
    11101 ggagttatcg agcagttatc cactctacat caccacgaca aagaatacat ctactgctca
    11161 agacatggcc aaaaacttgg aaatccatca tttctttgat ggtatttatg gttctagccc
    11221 tgaagcaccc cataaggcag atgtcattca ccaagcctta cagacacatc aactagcacc
    11281 agaacaagcc atcatcatcg gagataccaa gtttgatatg ctgggagctc gagaaacagg
    11341 tattcagaaa ttggccgtca cttggggatt tggagagcaa gcagatctac taaactatca
    11401 acctgattat atcgctcaca aacccttaga agttttggcg tattttcaat agcatagact
    11461 gggtaacaac tccatttcag aagaaaatga agtaatactc aatgaaaatc aaagagcaaa
    11521 ctaggaaact agctgcgggc tgctcaaaac actgttttaa gattgtagat ggaactgacg
    11581 aagtcagctc aaaacactgt tttaaggttg tggatggaac tgacgaagtc agctcaaaac
    11641 actgttttga ggttgcagat agaactgacg aagtcagctc aaaacactgt tttaaggttg
    11701 tagatggaac tgacgaagtc agtaaccata tacggcaagg cgacgttgac gtgatttgaa
    11761 gagattttcg aagagtataa gtctctacat aataaaacgc atattaccaa ggttgtttaa
    11821 cttgataata tgcgtttttc ttttttattc aatgtgggtt gtttgattag caaaggcgat
    11881 aatagcttgc ttcaactcat ctccactgag agtgcggaac tctccaatct tttgattgat
    11941 ggattctaaa ggcatgacat taaccttacc aacgaaaatc ttatccataa cttgattgtc
    12001 aacgagttcg gttgatacca agagttcttc gtagagtttt tcacctgggc ggattccaac
    12061 ttcaacgatt ggaatttcac tctcagtgtg gccacttaga agcaccatct tcttggccaa
    12121 gtcataaatc ttgactggtt tgcccatatc aaggataaag acttccccat ctttggcata
    12181 agcaccagca tggataacca gacgactagc ttctggaatg gtcataaagt aacgggtcat
    12241 gcggaagtct gtcaccgtta caggcccacc ttcagcaatc tgacgttcaa agactggaat
    12301 gacactacca cggctaccaa gaacattccc aaaacgaact gcacagtagg ttgattggct
    12361 acgttggtta aagccagtga caatcaactc cgccacgcgc ttggttgctc ccataacatt
    12421 tggtggatta actgccttat ctgtcgaaat cataaccatc ttaggcacct tggcttcatc
    12481 aacggctcta gcaacattgt aagttccacg gatattgttt ttgaaggctt cttttggatt
    12541 gcgctccatc ataggaacat gcttgtgggc tgccgcatga taaacaatag caggtttgta
    12601 ctgctcaaag acttgcaaca aacgatcata gtcttgaatg tccgcaatca caggtacata
    12661 atcaatccct tggaacttac gaatcaattc atgataaaca aggtagattg agttttcccc
    12721 atgaccgagc aagacaatgc gttcaggatt gaagcgacta acttgacgac agatttcaga
    12781 accgattgaa cctccagctc ctgtgactaa gatggtctta cctgtcagtt ctgcacccag
    12841 acgcgattcg tcaagacgga tttcctgacg acccaaaagg tccgtaatat caattttttg
    12901 gaagccagta cctgcttggt gaaggccctg aacaacagtt tcaaccttag gcatcttgta
    12961 acatttgaca cccagcttat tacacatctg caagatacgc tcatattctg acggatccag
    13021 cgacggaatc gcaacgatga cacgctcgat ttgatggcgt ttggctaatt caggcagatt
    13081 gtcataagag cccaaaacag gaataccacc aagtttttga ccctttttct tagaatcctt
    13141 atccaaaata ccgaccagtt ctaattcact ggttggatgt tggtaactat ccataaagag
    13201 agccccacca tcaccggcac caatcaagaa ggtccgacgg tgttctccat caccactacc
    13261 ttttttgcgt ctggagtaga ttaactgcca agtaatccgt ggcaataaaa tcaagaaggt
    13321 actcaacaag ataaagagaa tgatgaaacg gatggagaag agtggcaaga aggcataaca
    13381 gatactatat gacaagacac tgctagcagt cacaccaaaa aagattttca tgaaatccgt
    13441 aatcttgctg taacgactaa tgctcgcgtt caacccccaa aaaccaatca tcaattgata
    13501 gaacaagaag gccaaactcg tatagataat gtagtcaaca ggtgctggat taatcagccc
    13561 ataaaataaa atataagata caattatgga aaccaccata ctgaaaatat caaatattcc
    13621 ccagaaaacc tgcttttgtt gtttatttaa aatttccacc agatcaatca cataatctgt
    13681 tagtttttta ttcatagaaa tctactcctc
//
LOCUS       DAVLXF010000048        13530 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLXF010000048 DAVLXF010000000
VERSION     DAVLXF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.048"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(58..819)
                     /locus_tag="VYO12_001352"
     CDS             complement(58..819)
                     /locus_tag="VYO12_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1587955.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(852..1478)
                     /locus_tag="VYO12_001353"
     CDS             complement(852..1478)
                     /locus_tag="VYO12_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1587956.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(1494..1811)
                     /locus_tag="VYO12_001354"
     CDS             complement(1494..1811)
                     /locus_tag="VYO12_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1587957.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(1808..2107)
                     /locus_tag="VYO12_001355"
     CDS             complement(1808..2107)
                     /locus_tag="VYO12_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1587958.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(2392..2583)
                     /locus_tag="VYO12_001356"
     CDS             complement(2392..2583)
                     /locus_tag="VYO12_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV1587959.1"
                     /translation="MSYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGAMIANDWVKDNGK"
     gene            complement(2744..3418)
                     /locus_tag="VYO12_001357"
     CDS             complement(2744..3418)
                     /locus_tag="VYO12_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587960.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLAIIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(3424..3870)
                     /locus_tag="VYO12_001358"
     CDS             complement(3424..3870)
                     /locus_tag="VYO12_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1587961.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(3984..4487)
                     /locus_tag="VYO12_001359"
     CDS             complement(3984..4487)
                     /locus_tag="VYO12_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1587962.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVFIPATGFVILSLLRKKINAPRPYEEWDIKPLLDRDSPGQSM
                     PSRHVFSATIISMTCLHASLTVGFILLVLSALLGLVRVLGGVHYPKDVVVGYICGLAW
                     GVLFFLF"
     gene            complement(4484..4846)
                     /locus_tag="VYO12_001360"
     CDS             complement(4484..4846)
                     /locus_tag="VYO12_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1587963.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGEAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(4972..6771)
                     /locus_tag="VYO12_001361"
                     /pseudo
     CDS             complement(4972..6771)
                     /locus_tag="VYO12_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(6810..7259)
                     /locus_tag="VYO12_001362"
                     /pseudo
     CDS             complement(6810..7259)
                     /locus_tag="VYO12_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(7383..8099)
                     /locus_tag="VYO12_001363"
     CDS             complement(7383..8099)
                     /locus_tag="VYO12_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1587964.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(8980..10395)
                     /gene="ply"
                     /locus_tag="VYO12_001364"
     CDS             complement(8980..10395)
                     /gene="ply"
                     /locus_tag="VYO12_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1587965.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(10406..10738)
                     /locus_tag="VYO12_001365"
     CDS             complement(10406..10738)
                     /locus_tag="VYO12_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587966.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(10809..11417)
                     /locus_tag="VYO12_001366"
     CDS             complement(10809..11417)
                     /locus_tag="VYO12_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587967.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(11430..11888)
                     /locus_tag="VYO12_001367"
     CDS             complement(11430..11888)
                     /locus_tag="VYO12_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1587968.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            12013..12412
                     /locus_tag="VYO12_001368"
                     /pseudo
     CDS             12013..12412
                     /locus_tag="VYO12_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13176..13436)
                     /locus_tag="VYO12_001369"
     CDS             complement(13176..13436)
                     /locus_tag="VYO12_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1587969.1"
                     /translation="MRERSATGAQDLSKSIKKHLNDITRLTASLLGDEKLSAIISSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT         4086 a         3061 c         2405 g         3978 t
ORIGIN      
        1 ctattcgtag gaaatcaaaa aaagttacag aaatttgtaa cttttcgagg aaatttctta
       61 ttttttatga atcatgaaac ctgtcgcctg ttgattggcc ataatggtca tatctgtaat
      121 ctgaacacgg cgaggctgac tggtcacata gactactgtg tctgcaatat cctgagcttg
      181 caaggcttct attccttggt aaacggacgc agcccgctct ttatcaccat gaaaacgaac
      241 agttgagaaa tctgtttcga caatcccagg ctgaatggtt gtcaccttga tatccgttgc
      301 gatggtatca attcgcagtc catccgaaaa ggtcttaacc gcagccttgg tagctgagta
      361 aacagcggca ccagcatagg cgtagattcc tgcggttgac cccatattga taatatgacc
      421 ttgattgact tttatcattg ctggcaagaa acagcgagta accgccatca gacctttaac
      481 attggtatcc aacatggtca acatatccaa ctcctcatag tcttgatagg gagccaagtc
      541 aagagccagt ccggcgttgt tgaccaagat atcaatcctc cctatcgttt ctagaatatc
      601 agagcaaaca gtcttcacca tgaccatatc cgtcacatct agtggaaaag tccagacggt
      661 ttgatttgga aagcttactg caaactccga cttgaggatt tctaatctgt ctatccgtcg
      721 tcctgttaga acgacatcct caccctgctc cagataagca cgcgcaatcg cttcaccgat
      781 tcctgaggtt gctcccgtaa tcactacatt ttttgccatc ttatttcctt ctatcagatg
      841 ctgacaagtt cctaggcagt ccagtgtttc gctgggtcga acggtgttcc gacaacttgg
      901 tcttctgata attcaagcac cccacgtttt tgcggagcat ttggcaaatg caattcacga
      961 ggactacaca tcatcccaaa actcttttca ccacgaagtt cgcctgggaa aatgaaattc
     1021 ccttttggca tcatggcacc aggaagagcc acaatggttt tcaacccaac acgcgcatta
     1081 ggtgctcctg caacgatttg cactatcttg tcacttgcga ctgcaacttg gcagatgttg
     1141 aggtggtcac tgtctggatg agctaccatc tcgacaattt caccaacaac aaacttaggt
     1201 tccttatcat taacaatttc ttctgtaaaa ccttccgcct gtaattcttg gttcaaacga
     1261 gcgacttgct catctgataa aaagacttga ccgcgctctg caatttcaaa caagctcgaa
     1321 acctcaaaaa tgttccaagc tactgtttcc ccattttctt tgaggaaaac acgggctacc
     1381 ttgcctttgc gttccacatt cagtttggca tctccgctat ttttcacgat gaccataagg
     1441 acatcaccga catgttcttt gttatacgta aaaatcattg tttccttttt ctcctatttc
     1501 aatcctgcta aaaaatcgtt aatttgctcc ttacttttac ggtcgcgatt gacaaaacga
     1561 ccgatttcct tgtccttttc tagaacaaca aggctaggaa ttccgtaaac atcccagagt
     1621 ttggccaaat ccatatactg atctcggtcc attcgaataa aggtgaactc tggattggtc
     1681 tcttcaatct ctggtaaggc aggataaata taacgacaat cgccacacca gtctgccaca
     1741 aaaaggaaga ccttcttgcc cgctttttcc actaaagatg ctaattcttc taaacttgct
     1801 ggctgtatca taagacttcc tcctcataga ctaggtcttc attttcatag acaaaggtat
     1861 aatgacggcc atcctcaaaa atgacgccac caagcaagct ctctagactg ctttcataga
     1921 cttgaacata gagggtcgca atttccccca tgtctgaaaa atggtctcgc acaatctctg
     1981 tcaactcttc ctgagtcttc atgagcttac ggtcatctgc aacttttttc gtagcaagag
     2041 caaggcttcc gatacctagc agagccaagc ctgccatcca cattttttta gctttcatac
     2101 cattcatttt aacacaaaaa aggcttcagg acaaatgagg aagcagcaga aaagcaagta
     2161 aaaagcctct tcctttaagg aaaaggactt cttatactaa gagcaaacta gaaagctagc
     2221 cgcaggctgc tcaaaacacg gttttgaggt tgtagataag gcgacgttga cgtggtttga
     2281 agagattttc aaagagtatt attcttattg ccaggcacct aagttgccaa cgtagtaacc
     2341 atcaggtgtg taggtattgc gaagcatctt acctgatgaa gccagataat actacttgcc
     2401 attgtctttg acccaatcat tcgcaatcat ggcaccagaa gaacttacat aataccattc
     2461 tcccttgtca taaacccaag tactgacttt catggttcct gagcaattaa aggcaaaaaa
     2521 actgtccaat atcattcgtt ttttaaaagc atttgacact acatcatcta cgctgtagga
     2581 catagatgca tcaaacgtaa aagccaaaat atcgaaaaat atacaacttt taaaggcagc
     2641 atttcatttt ttaaagtcta acaaccatag aactgaaatc agagcaaggc atcaatttcc
     2701 tactcttagt acgtctattt tgatggttga agtgaagatt tttttattta acgtagacaa
     2761 tttccaactt cccaaaagcc acatctccac ggataatcaa ggttttgctg gttggggcta
     2821 caggagcgtc agccttagct gcaccaaagg aggtttctac tttcaaatct acacgccagt
     2881 gttgtggaac atagacggtt gcatttccga aggccacttc aatatttaaa gttgcaaaat
     2941 cacctagcat ctctgcatta tcatagtaga ttttagcatc cccaaaagcg acttccactt
     3001 ggtcatctac gagatcttga tcttgcttat agaaggtccc gctaccaaaa gcgacttcct
     3061 tatccgtgac gactgttttt ttcccattgt accaccattt tttttcattc cagaacttac
     3121 ttgaatgcgt cagataacca acaccaagta ccaccaagat gctagcccaa ataagagaat
     3181 gattggtaac tggtaacaag tcataagcgt aatttgcaat gatgatcgcc agtaaacctg
     3241 taaaaactgc cgaagtgaga tgacgtctaa ggatggactc aatggactta taagcaaaaa
     3301 aaacaatacc tagtaaaggc catattttac catccaaaga aggaattcca aaattccctt
     3361 gcagcaagat ccaagctgct aaaaccaata aaacaatacc aaatgctttc tttttcatgt
     3421 tttttacctc atgtttctta gattttcttt taggagggat tggtaatgcc gtgagacatg
     3481 aacctccttg tgcgtctgat aaaaggaaat ggtgcccgtt cctgaaaagg acttgtccac
     3541 tgagtaaatc tgacggatgt ttgcgatcgt tgacttggaa actcgattaa aatagcgagg
     3601 caagatagac tccaactcat agagcttgag gcgaacctca taagcttcct tctggttatg
     3661 agcgtagatc ttgctccctt ctgtttcaaa gaagagaatt tctgacaggt ctagataata
     3721 ttcacctgtc cccttgtaaa aagtcaacct cggagccttt gactcttgca agagccgttg
     3781 caagtctgca atttcatctg tcaaagtggg ggtcttgatg acaatttcag tctcctctaa
     3841 attgccgtca atctcaattc gtaacttcat cacatctcct ttctgactct actatatcaa
     3901 agctaagtta ggaatacaag ggcaaaaaag caagtggtca atttgaagca gtaagtggtt
     3961 gcaagaccaa cttaacttac aggtcaaaat agaaagaaaa gcacacccca cgcaagacca
     4021 caaatataac caactactac atccttggga taatgtacac cacctaagac tctcacgaga
     4081 ccgaggaggg ctgacaagac caacaagata aagcccacag ttagactagc atgtaagcaa
     4141 gtcatggaga taatggttgc tgaaaagaca tgacgactgg gcatagactg accaggacta
     4201 tctctgtcaa gcaagggttt aatatcccat tcctcataag gcctaggggc attgattttc
     4261 ttacgaagaa gggataaaat cacaaaacct gtagcaggga taaacacata aaccccaact
     4321 tgctgaccaa gtcctagttg caggtaagtg gtgactagca agatcagata gaccaggggc
     4381 atggcgactg tcatcaaacg attgaaactg cgtaatagga ataaaagaat tggacgacta
     4441 gttagtctgg agctgatatt tcgataccat tcttgataat ttttcattta tgcttctcat
     4501 acttttctag aacttgttga gatgttttcc ccttggcaag ccaatccact agcttgtcca
     4561 agcgacgtat ttcctgcatc agaggatcat caatttcctc aatacggaca ccgcaaatct
     4621 tccctgtaat tgcaattcgt tcagggacat aatatggtgc ttgtcgaaaa aaatctccgt
     4681 aagtaacatc cctatccaga caagcaagaa cgtcactcac ttcataccct gtcaaccaac
     4741 tggtcacttg gtgaactgac tctgcctctc caccctttcg ctcaactttt gcaatcaagg
     4801 ctaaatagac agcagcaaat ttcatattgt ataatttctg actcatactt ctctctttct
     4861 atgtgtacta gtataaataa gaaaaagaag gccgtcaagc cttcttttga tttattcttc
     4921 tgcttcatct tctgtaaatt gactattgta caagtcagcg tagaagccac cttacaccat
     4981 cagttcctca tagttgcctt gctcgatgat atttccatct ttcatgacca agatcaagtc
     5041 tgcatttcgg atggttgaca agcggtgggc aatgacaaag gatgtgcgtc cttccatcaa
     5101 acggtccatg gctttttgga tcaattcctc tgtccgtgtg tcaacagaag aagtcgcctc
     5161 atccaaaatc aaaagcggtg catccttaag aagggcacga gcaatagtca atagttgttt
     5221 ttgtcctaca gacaaggtca cggtgtcatc caagatggta tcatagccat ctggcaaggt
     5281 cataataaag tggtgaattc ccacagcctt actagcttcc atcattcgtt catcactaat
     5341 ccctatttga ttatagatga gattgtctcg aatagttcct tcaaagagcc aggtatcctg
     5401 caagaccatt gaaaaggcat catgcacttc tgaacgcgtc atagccttgg tatccacacc
     5461 atcaatgcga atacttccct tatcaatctc atagaatttc atcaaaagat tgacaatggt
     5521 tgtcttacca gccccagtcg gcccaacaat ggcaaccctt tgaccagcat gagctgtcgc
     5581 agagaagtca tagtcttgaa cattgacacc gtccaccaga atttctcctg ctgacacgtc
     5641 gtagaaacgt ggaatcagat tgaccagagt tgatttacca gaacctattg acccaataaa
     5701 ggccactgtt tgaccagttt ctgctttaaa gctaacatgt tcaataactg cctccgaatt
     5761 tgccgcatag cggaaggtca catccttaaa ctcgacctga cctttgaagt tttcatcagt
     5821 cagctgcact tgagcagggt tttggataga agaatgcaaa tctaaaactt gattaatccg
     5881 cttagcagag accatagttc ggggaagaac gatgaagagt gctcccatga gaaggaagtc
     5941 catgacaacc tacatggcat aagacatgaa aacaatcatg tcactaaaga gaggcagacg
     6001 cgctatcgga gcagcgtcgt taatcacata ggccccaatc cagtaaatcg ccacactcaa
     6061 accacttgaa atccccatca tgataggatt caaaatagcc ataagacggt tgacaaacaa
     6121 attttaacgg gtcaattcat catttactgc tgcaaatttt tcattttgat aatcctctgc
     6181 attgtaggca cgaacgacac gaatacctgt taaactctca cgagtgatac tgttcagttt
     6241 atctgtcagc ccctgaatca aggactgttt tggaaaggct agcgtcatca aaacggtcgt
     6301 catcaggacg ttgacaatca ctgccacaag tacggcccag agccagtatt ctgaatgacc
     6361 taaaatcttc ccaatagccc agatagccat aattggacca cacgttacca cttgcaagtc
     6421 catagtaatc aacatttgaa cttgagtaat gtcattggta gtacgcatca agaggctagg
     6481 aattgaaaat ttcttaatct ctgtctgcga gtaatccaaa actcggttaa aaatatcact
     6541 tctcagccta ctagtataag aagccgccac tcgggatgca aaaaatccaa ctgcaactac
     6601 ggacaagaag gcaagaaagg gcattcccat catcatgctt gccggctgcc acaactcatc
     6661 taaattagtt tcttgactac ctagcaaatc cgtaattttc gagatatagg tcggcacttc
     6721 caactctaga tagaccgaaa agcaagtaaa gagaatggct agtaaaatca tcccccattc
     6781 ttttctacta attcttttgg ttaatttctt tattctctcc tcctattccc ttgatatttt
     6841 gcctgtagtt gaccgagaac tttctcaaaa atcagtaatt catcttcatc aatgtcttcc
     6901 atcaactgct tgtctatgcg ttcaaaaaaa gccttaacct gttgcatctg agaacgtgct
     6961 ttgtccgtca gacgaacaaa cttagcccgc ttatcaacag gactcgcctc caattccacc
     7021 aaaccatttt gcactatacg cttaaccaga ttactagcaa cagacttggt aatattgagt
     7081 tcctgctcga tatctttaat caagaccaag tcttgatttt tctagcgatt atccaaaaaa
     7141 cgcacaacct gaccttgcgg cccacccata aattcaatgc cgcaacgttt ggcttccttt
     7201 tgcaccatca ggtaaatttg atgaccaaaa cgcttaaaga ctaacatcgg tttatccata
     7261 atctccccct tctaaataaa aatagttctc tggagaataa ttaaatttct atgagaacta
     7321 ttttcttgat taaaaaaatc ccaagtgatt ttctcactta ggatcatgtt ctataggtta
     7381 aattaaaacc catctacgtt cgtataaatc ttttggacgt cttcgtcgtc ttcaagaacg
     7441 ctgtaaagtt tttcaaaggt ttcaaggtct tcgcctgaca attccacttc tgactgagga
     7501 atcatttcca attcagtcac ttggaattct tcaataccag actcacggag agcaacgata
     7561 gccttgtgaa ggtcagttgg cgctgtgtaa actgtgattg taccttcttg tgcttctacg
     7621 tcatccacat ccacatccgc ttcgagcaat tgctcaaaga ctgcgtccgc atcttcacct
     7681 ccaaatacaa taacaccttt gttgtcaaaa aggtaagaaa cagaacctga agcgcccatg
     7741 tttccgccgt ttttaccaaa ggctgcacgg acattggctg ctgtacggtt aacgttagaa
     7801 gtcaaagtat ccacaattag catagagcca tttggcccaa aaccttcgta acgtccttct
     7861 gtaaaggttt cgtctgtgtt tcctttggct ttatcaatcg ctttatcgat aatgtgtttt
     7921 ggcacttggg cttgtttagc acggtcgata acgaatttca aagctgagtt tgattctgga
     7981 tctggatcac cttttttagc tgctacatag atttctacac caaattttgc atatacttta
     8041 gagttagctc catctttagc cgttttcttg gctacgatat tggcccattt acgtcccatt
     8101 aggaatctcc ttttttcaca ttttaatctt tcttattata acacaagttt ttttgatttt
     8161 cactagagga aatggatttt attagcaaat caagctagga tagcacttta cctgctaaga
     8221 tggtcttgcc tttctatctt tatcaacaga cactcatcca cattcaaaaa acaaactaga
     8281 ccattatctg caaatagaaa gtttcagcca agtttgacaa agtcagctca aattactgtt
     8341 tgaagtttgt agatataagc gacaaaaaca atcatactgc accttttgtt gacagtctac
     8401 tccagacata tcatagttca agtaaatact ttgaaattca acagttctta taggcgctat
     8461 tgtattctaa gaaatcaata gaagagtttc taagcaaacc tctaatactc aataaaaatc
     8521 aaaaagcaaa ctagaaagct agccgcaggt tgctcaaaac actgttttga ggttgcggat
     8581 aggactgacc tggtttgaag agattttcga agagtataat ttacgtgttc ccaagatgga
     8641 gaaattagac tagtacactg gcacttctaa accattgcta gcaattgatt tgttcatatt
     8701 taatttcatt ttttccataa atgggtatta gatataaaca gcaaaatatt tccgatacgt
     8761 gtcattcttg aatttccaat catctaaaac aagtaaagga taatcaatcc cctgtatatc
     8821 aaggaattgg ctaccctttt tactttttta cacattctgt ttgatagatt cattttaaca
     8881 tcacgagcat actccaatgg aaatcgctag gcaagagata aactttcaga tattcgcaga
     8941 gagctcatcg cctctttttg tcgcaaacat tcttctctcc taatcatttt ctaccttatc
     9001 ctctacctga ggatagagag ttgttcccca aatagaaatc gtccgcttac gcactagtgg
     9061 caaatcggtt ttttcataaa ccgtacgcca ccattcccag gcaagcccgg tacactctct
     9121 aattttgaca gagagattac gaacattccc ttttaaagga atactagtgg taaagtgagc
     9181 cgtcaaatcc tgcccatttc tgtcccaagc cttaggagtc aagacttcct taccttgatg
     9241 atcataggat aattcatccc aagtaatata atattgggca acataggcac cactatgatc
     9301 cagcagtaaa tctccgtttc tgtaagctgt aaccttagtc tcaacatagt ctgtactatt
     9361 ttgaaaggtc gcaactacat tgtcacgtaa aaaagaagtt gtataggaaa tcggcaagcc
     9421 tggatgatct gctgtaaagc gactgccttc ttgaatcaag tcctctacca tatccacctt
     9481 gcctgttaca actcgggcac ccgaacttgg gtcgccccct aaaataaccg ccttcacttc
     9541 tgtattgtcc aaaatctgct tccactctgt ctgaggagct accttgactc cttttatcaa
     9601 agcttcaaaa gcagcctcta cttcatcact cttactcgtg gtttccaact tgagatagac
     9661 ttggcgccca taagcaacac tcgaaatata gaccaaagga cgctctgcag aaattcctct
     9721 ctgttttaaa tcctctaccg ttacagtatc ttgaaacaca tctcctggat ttttaacagc
     9781 atctacgctg actgtataat aaatctgctt aaaattaaca atctgaatct gcttttcgcc
     9841 tgaatggaca gagttaaaat caatatcaag agaattccct gtcttttcaa agtcagaacc
     9901 aaacttgacc ttgagttgtt ccatgctgtg agccgtgatt ttttcatact gcattctagc
     9961 tgggacatta ttgacctgac cataatcttg atgccactta gccaacaaat cgtttaccgc
    10021 tccgcgaaca cttgaattgc tggggtcttc cacttggaga aagctatcgc tacttgccaa
    10081 accaggcaaa tcaatactat aagtcatcgg agcacgatcg accgcaagaa gagtgggatt
    10141 attctctaac aaggtctcat ccactacgag aagtgctcca ggatagaggc gactgtcgtt
    10201 ggtagctgtt acagaaatat cacttgtatt tgtcgacaag ctccgcttct ttctttcgat
    10261 aacaacaaac tcatcgggta gctgattacc ctctttgatg aaacgatttt caatactttc
    10321 tccctgatgg gtcaagagtt tctttttatc gtaattcata gctagtataa agtcatttac
    10381 tgctttattt gccatcttat acctcctaat aagttcctgg attgagttgc ataaactcag
    10441 acttgttcag cgaaatcagc cgtggttgga ctaagtgatc caaaatttcc tcgtacaatt
    10501 cttctgagac attgcgtcgc cgtctggcta gataagaagt cggaataacc gtattatcca
    10561 acataaatac cttatctaag tcaatcaagg ttggtcttgt aaaaggatta cgagctagat
    10621 ccggctcttc tatcataaag ttcttgacca aacgtctggt caagagagct ggtttgaagg
    10681 tctgattttt aaccaactct ttgtttttag tcatgctgtt gtcaatacag atatacatat
    10741 gattcttcac agccaaatcg ctactaatag tcggaaaagg caaataaaga gctacaacat
    10801 ctcctctctt aatcaagcaa gagcaccccc ttttctccta atgtaacata gacaggattg
    10861 accaagtctt ctgattgact cagaatttcc aaagtttgag tttggcgcgc tgtcaattta
    10921 gtagcatctt gtctcttcaa tacaaaatgc ttgtcgccaa taaccttgac aatataatcc
    10981 ttctccaaag ctgactggta aatccacatc agatgttgtc tgtcctgaga actcaagaga
    11041 gaaggatttt caagcctccc gatagtctga taaaaatcaa aaacaggagc taactcctgc
    11101 caatctgatt ggctagttgt caaggctaga aaaagggctt tgcgagctga tacttcttgg
    11161 ttagccttga gagttacttt cccctccaag ttttttagaa atcgggaaac tccagaaagc
    11221 aaatttttct ctaactgcga gaaataaaaa cctttcgttc ccagacataa gtctttcatg
    11281 tcgctttctc tagcaaataa gagctcaaac atttgatagt aaaagaaaaa tatctggcac
    11341 tgggtcgcgc tcatcttttc cttatcggct tcttttttta accagagcaa gggcgacagg
    11401 tagctggatt gagacatttc ctctacctcc tactcttttt taactggagc atctgcacta
    11461 gctgccactt cttttgactg gatactttcc cactggttaa tctcctctga gataagacct
    11521 tcgcatgtct tgacaaatag ggcaaaagcc ttggtctttc ctgcatattt ctccgtttgg
    11581 cattgataga ggaatttttc tttctccagg agttgcgcag ttttttggta agaaatccaa
    11641 ttttcctttg cattatacaa attgataatc ccctcacaca gcaagccgag actggataag
    11701 gcaaccgaaa tcaaacggta gcgatcacct ggcataggaa tagcacaaaa gacagctatg
    11761 aggaaacctg ccacgatttc tgttattttt aataccttat agcgcctacg atgttgaacg
    11821 cttttcttta aaaaatgagc tatctgtacg tctaatcgct ctgtcaggta catttcttct
    11881 ggcgtcatat tcgtaactcc tttcatttac tttgataacc atagtataac atatttgaat
    11941 atcaaagtat agtaaaaact ggactataac ttattagact ccctgcgaaa caaaatatgg
    12001 tatagtagtt ctatgaatta tgaagcaagt aaacaattaa ctgatacacg atttaagcgt
    12061 cttgttggtg ttcagcgtac cacttttgaa gagatgttag ctgtattaaa aacagcttat
    12121 caaaaaagtc ggacaagctg gtaaaatctt ggctgacagt aattatcaag ggctcatgaa
    12181 gatatatcct caagcacaaa ctccacgtaa atccagcaaa ctcaagccac taacagttga
    12241 agataaagtc tataaccatg cgctatctaa ggagagaagc aaggttgaga acatctttgc
    12301 caaagtaaaa acgtttaaaa tgttttcaac aacctgtcga aatcatcgta aacgcttcgg
    12361 attacgaatg aatttgagtg ctggtattat caatcatgaa ctaggattct agttttgcag
    12421 gaagtctatt atatatatgc acagacatat gaaatctttt tgatacagcc ttctttttac
    12481 atagagagac agcagttatg atatatttat ccattcacaa catcttctgt accaatacta
    12541 caatattggt acattttgat tacacaatcg tttcaaaaaa tatgatggaa gcctgagtat
    12601 tttatagtaa ttcccttcct atcaactaga tcaaccagtt gagtgttgaa tgttgaaaca
    12661 ccctagcctc agcaaaggac tatgatcagc atttcctagc caataggacg ctccaaatca
    12721 cctattctaa gtccatcact agcttggtca gtaagataca gactgcaagg caagactgac
    12781 gtggttgatg agattttgga agagtattac aatcatagat tcaccagtag acttgaccgc
    12841 agatttaact atagtagatt gaaactagaa tagtacactt ctgcttctaa aacattgtta
    12901 gaaatcgatt tgactgtcct gatcaatttg tcctgttctc gtttcatttt actatactaa
    12961 aaaaccacct gaaacaaatc aagtgactag aggataaaaa caaagactgg gcaaaaagtt
    13021 ttgaaaagca aaaaacgcat aatatcaggt cttgaaaaac cttgatacta tacgtttgat
    13081 tgtgggaaga ttgacttcat tttctcctga aattgaagtt ttttctagtt tccagatata
    13141 gacgaattca atatagccat ctcgctacaa ttattttaac catcgagaaa atttttcaga
    13201 atttcaaaaa tttcattttg atccaatgaa atgtcatcat tttgaagaat agttgacttc
    13261 acaggctcta attctatcac aaagcggtgc atgtctgctt ttaccgcact acttgatatt
    13321 atagccgata acttttcatc tcctagcaag gaagctgtca aacgggtaat gtcattcaaa
    13381 tgctttttaa tggacttact taaatcttga gcacctgtag cagagcgctc tctcatatcc
    13441 agccaagctt tagccttaaa aataattaat actcaatgaa aatcaaagag caaactagga
    13501 agctagctgc aggctgctca aaacactgtt
//
LOCUS       DAVLXF010000049        13342 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLXF010000049 DAVLXF010000000
VERSION     DAVLXF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.049"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..516
                     /locus_tag="VYO12_001370"
                     /pseudo
     CDS             1..516
                     /locus_tag="VYO12_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            708..2999
                     /gene="pcrA"
                     /locus_tag="VYO12_001371"
     CDS             708..2999
                     /gene="pcrA"
                     /locus_tag="VYO12_001371"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1587970.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            3042..3722
                     /gene="radC"
                     /locus_tag="VYO12_001372"
     CDS             3042..3722
                     /gene="radC"
                     /locus_tag="VYO12_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1587971.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3719..4408)
                     /locus_tag="VYO12_001373"
     CDS             complement(3719..4408)
                     /locus_tag="VYO12_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1587972.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4426..5067)
                     /locus_tag="VYO12_001374"
     CDS             complement(4426..5067)
                     /locus_tag="VYO12_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1587973.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(5150..5365)
                     /locus_tag="VYO12_001375"
     CDS             complement(5150..5365)
                     /locus_tag="VYO12_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1587974.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(5373..5720)
                     /locus_tag="VYO12_001376"
     CDS             complement(5373..5720)
                     /locus_tag="VYO12_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1587975.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5725..6840)
                     /locus_tag="VYO12_001377"
     CDS             complement(5725..6840)
                     /locus_tag="VYO12_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1587976.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6850..7809)
                     /locus_tag="VYO12_001378"
     CDS             complement(6850..7809)
                     /locus_tag="VYO12_001378"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1587977.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7922..8491)
                     /locus_tag="VYO12_001379"
     CDS             complement(7922..8491)
                     /locus_tag="VYO12_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1587978.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8619..9290
                     /locus_tag="VYO12_001380"
     CDS             8619..9290
                     /locus_tag="VYO12_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1587979.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            9274..10092
                     /locus_tag="VYO12_001381"
     CDS             9274..10092
                     /locus_tag="VYO12_001381"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1587980.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            10089..10985
                     /locus_tag="VYO12_001382"
     CDS             10089..10985
                     /locus_tag="VYO12_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1587981.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            11029..12003
                     /gene="pta"
                     /locus_tag="VYO12_001383"
     CDS             11029..12003
                     /gene="pta"
                     /locus_tag="VYO12_001383"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1587982.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(12319..13339)
                     /locus_tag="VYO12_001384"
                     /pseudo
     CDS             complement(12319..13339)
                     /locus_tag="VYO12_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3901 a         2531 c         2878 g         4032 t
ORIGIN      
        1 gttttacgat ttcaagaact cctctcagtt ctgaggacac ggtaatgatt gatgcgacgg
       61 aagtaaaaat caatcgccct aaaaaaagaa ttagcgaatt attctggtaa aaagaaatgc
      121 cacgctatga aggctcaagc gattgtcaca agtcaaggga gaattgtttc tttggatatc
      181 gctgtgaact attgtcatga tatgaagttg ttcaaaatga gtcgcagaaa tatcggacaa
      241 gctggaaaaa tcttggctga cagtggttat caagggctca tgaagatata tcctcaagca
      301 caaactccac gtaaatccag caaattcaag ccgctaacag ctgaagataa agcctataac
      361 catgcgctat ccaaggagag aagcaaggtt gagaacatct ttgccaaagt aaaaacgttt
      421 aaaatgtttt caacaaccta tcgaaatcat cgtaaacgct tcggattacg aatgaatttg
      481 attgctggca ttatcaatca tgaactagga ttctagtttt gcaggaagtc tattgaggta
      541 ttgagctagt ttatgaaaaa attgggtgaa aaagtcgagt gttttagaaa cccacagtgt
      601 agtattctag tttcaatcca ctatattttg ctactccccg taaagtttct attttccctg
      661 atttctgata taatagaaat attgacttca agagtaagga agagaagatg aacgcattat
      721 taaatggaat gaatgaccgt caggctgagg cggtgcaaac gacagaaggt cccttgctaa
      781 tcatggcagg agctggttct ggaaagactc gtgttttaac ccaccgtatc gcttatttga
      841 ttgatgaaaa gctggtcaat ccttggaata tcttggccat tacctttacc aacaaggctg
      901 cgcgtgagat gaaagagcgt gcttatagcc tcaatccagc tactcaggac tgtctgattg
      961 cgaccttcca ctccatgtgt gtgcgtattt tgcgtcgcga tgcggaccat attggctaca
     1021 atcgtaattt tacaattgtg gatcctggtg aacagcgaac gctcatgaaa cgtattctca
     1081 aacagttgaa cttggatcct aaaaaatgga atgaacgaac tattttgggg accatttcca
     1141 atgctaagaa tgatttgatt gatgatgttg cttatgctgc tcaagctggc gatatgtata
     1201 cgcaaattgt ggcccagtgt tatacaacct atcaaaaaga acttcgtcag tctgaatccg
     1261 ttgactttga tgatttgatt atgctgacct tgcgtctctt tgatcaaaat cctgatgttt
     1321 tgacctacta ccagcaaaaa ttccaataca tccacgttga tgagtaccaa gataccaacc
     1381 acgctcagta ccaattggtc aaactcttgg cttcccgttt taaaaatatc tgtgtggttg
     1441 gggatgcgga ccagtctatc tacggttggc gtggtgctga tatgcagaat atcttggact
     1501 ttgaaaagga ttaccccaaa gctaaggttg ttttgttgga ggaaaattac cgctcaacca
     1561 aaaccattct ccaagcggcc aacgaggtta ttaaaaataa taaaaatcgc cgtcctaaaa
     1621 atctctggac tcaaaacgct gatggggagc aaatcgttta ctatcgtgct gatgatgagc
     1681 tggatgaggc tgtatttgta gccagaacca tcgatgaact tagtcgcagt caaaacttcc
     1741 ttcataagga ttttgcagtt ctctatcgga ctaatgccca gtcccgtaca attgaggaag
     1801 ccctgctcaa gtctaacatt ccttatacca tggttggcgg aaccaaattc tacagccgta
     1861 aggaaattcg cgatattatt gcttatctca accttattgc taatttgagt gacaacatta
     1921 gttttgagcg tattatcaac gagcctaaac gtggaattgg tctaggtaca gttgagaaaa
     1981 tccgtgattt tgcaaatttg caaaatatgt ctatgctgga tgcttctgct aatattatgt
     2041 tgtctggtat caagggtaag gcagcccaat ctatctggga ttttgccaat atgatgcttg
     2101 atttgcggga gcagctagac cacttaagca ttacagagtt ggttgagtcc gtcctagaaa
     2161 aaacaggtta tgtcgatatt cttaacgccc aagcgactct agaaagcaag gcaagggttg
     2221 aaaatatcga agagtttctt tctgttacga agaactttga tgacaccact gatgtgacag
     2281 aagaggaaac tggtctggac aaactgagtc gtttcttaaa tgacttggct ttgattgccg
     2341 acacagattc aggtagtcag gagacatcag aagtgacctt gatgaccctg catgctgcca
     2401 aaggtctcga atttccagtt gtctttttga ttgggatgga agaaaatgtc tttccactta
     2461 gtcgtgcgac tgaagatcca gatgaattag aagaagagcg ccgtctagcc tatgtaggta
     2521 tcacgcgtgc agagaaaatt ctctatctga ccaatgccaa ctcacgcttg ctttttggtc
     2581 gtaccaatta taaccgtccg actcgtttta ttaacgaaat cagttcagac ttgcttgagt
     2641 atcaaggtct ggctcgtcct gcaaatacaa gctttaaggc atcatatagc agtggtagta
     2701 tttcctttgg tcaaggtatg agtttggctc aggctcttca agaccgtaaa cgcggtgctg
     2761 ccccaaaatc gatccagtca agcggtcttc catttggtca atttacagct ggcgcaaaat
     2821 cagcatctag cgaggcaaat tggtccattg gtgacattgc tctccacaag aaatggggag
     2881 agggaactgt tctggaagtt tcaggtagcg gtgctaggca ggaattgaaa atcaatttcc
     2941 cagaagtagg tttgaaaaaa cttttagcca gtgtggctcc aattgagaaa aaaatctaat
     3001 tttccatcct tctcacgaat aataaagtga ggaggatttt tatgtacagt atttcattcc
     3061 aagaagattc actattacca agagaaaggc tggccaagga aggagttgaa gcgcttagta
     3121 accaagagtt gctagctatt ttactcagga caggaacacg tcaagctagc gtttttgaaa
     3181 ttgcccaaaa agtcttgaac aatctttcaa gcctaacgga tttgaaaaaa atgaccctgc
     3241 aggaattgca gagtttgtct ggtattgggc gtgttaaggc catagaatta caagctatga
     3301 ttgaactggg gcatcgtatt cacaaacacg agactcttga aatggaaagt attctcagca
     3361 gtcaaaagtt ggccaagaag atgcagcagg aattagggga taaaaaacaa gagcacctgg
     3421 tggcactcta tctcaatact caaaatcaaa tcatccatca gcagaccatt tttatcggtt
     3481 ctgtaactcg tagtatcgct gaaccgcgag agattcttca ctatgcaatc aagcatatgg
     3541 cgacttctct tatcttggtc cacaatcatc cttcaggagc ggtagcgcct agccaaaatg
     3601 atgatcatgt cactaaactt gttaaagaag cctgcgaatt gatggggatt gttctcttgg
     3661 accatttgat tgtctctcat tctaattact ttagttatcg tgaaaagaca gatttaatct
     3721 aaagttcatt aacgacatag tcaaagagtt ttttatcttt gggacgattt tcaaaaagaa
     3781 gttctggatg ccattggaca ccgagaaagg cgacatcatc cgtactcatg acagcctcaa
     3841 tgataccatc tttaggatca tgagccacaa cttttaaatt tggtgctaag tccttgatgc
     3901 tctggtggtg gaaggagttg atatgagaga tttctccata gatttcttgg agaacggtat
     3961 ctggttctgt taccaagcgt tgagttgtgt actcaacaga agaatcctgc caatggtctt
     4021 cgatatcttg gtacaaagtt ccacccatgg caacgttaaa gagttgggta ccacggcaga
     4081 cagagaaaat gggctttttc tgtttaatag cttccttgat gagggccagt tcgaagatat
     4141 ctctttgaag gttatagtca tcgctatcaa tggttttggg ttcgccataa aattttggat
     4201 cgacattttg cccacctgtc aagatgagct tgtcaatcaa actgatatag tggcaggcca
     4261 tttcttgatc accaatcggt aggatgatgg gaatccctcc agcatcttta acgccttcaa
     4321 caaagccttt tgctgcgtag ctcatcatga tgtcatcatc tggatgagct ttttcgtttc
     4381 ctgtaatccc aataactggt tttttcataa aatgattttc gctttctaat cctcttttcg
     4441 catgaagtag aggagggttt ggagttcact tgtcaaatcg acatactgaa cgaccacgtc
     4501 ttttggtaaa tgcagatgga ctggtgaaaa actgagaatt cctttcacac cagcatcaac
     4561 caagagatta gcaacctctt gtgacttgac gctgggaaca gttaggatag cagtcttcac
     4621 atcagcatcc ttgattttat ccttgatctg agaaatcccg taaatgggaa tcccgtcagg
     4681 agtttgggta ccgacttcag gatggtcgtc taggtcaaag gccatgataa tcttcatctt
     4741 gttacgttcg tggaagcggt agtggagaag ggcatggccc atatttccaa taccaaccag
     4801 catgacattg gtaatagagt tgtcattgag caaatcggca aaaaatgtca ttagtttttt
     4861 gacatcatag ccaaaaccac gacgaccaag ttcaccaaaa taggaaaaat cacgacgtac
     4921 ggtcgctgaa tcaataccga tagcctctgc aatttgctta gagttggcac gttcaatctt
     4981 ttctgcatga aatctcttaa aaattcgata gtagagagag agtctttttg ctgtagcttt
     5041 tggaatagca aactgtttat ctttcacaaa atcacaacct ttctattctt ctattttata
     5101 gaaacattgt gaaaaaatca acaaaaataa gaaaaaacta agaaaaatct tagttttgat
     5161 gtaaaaaatc tgcatgagat agaaaacggt agaggtctcc gaccagaccc tgataaactt
     5221 tttggcccct aaaagtcaga gaagtcacat aaagtgtatc tggtaaggtt acacatcctg
     5281 acaaagtcaa catgagagcc tcatgatcct catacttgag agtacgctct acatgatagc
     5341 agtccttata ggtcagttca aacattttgg ctctatcttt ccgattttgt aaagacacca
     5401 cgttctacca agctatccat gaggaagtag aatttttcct gatgaatatg gtggtcttct
     5461 gatttgaaaa tatcaactag acgaaggcta aacttgtcag tgatattgat tttagcccct
     5521 gtaagttcct tgttaatgat gattttgagt tggaagcctt caccgctgtt tggcactttt
     5581 tccaaaaggc gagtcagttc atagttacca accttagttt caaaaaaggt gttatctttg
     5641 agggtgaatt ttttaacaga agggctaaga gtgtaatcgt aacgacaatt ttttaactga
     5701 atgatttttt caaatgccat atggctaacc tccgataatt tcttttaagg tttttgcgag
     5761 ggtttgtagg tcttcaacgg tattttgtgg cgacaaactg atgcgaaggg attccttcaa
     5821 gcgttctgaa tttgcgccat acatggcttc aagaacatgg ctggattgga caacgcctgc
     5881 agtacaggct gagccagtag agattgaaat tccagctaaa tctagccgaa ggagtaagag
     5941 gtcatttttc tgaccaggaa atccaatatt gagaacataa gggagatgat gttttcctct
     6001 attcaggtaa tactgaatgc cctccagctc tgccagaaag gcggtttcta gattttgtac
     6061 atgttgaaaa tgttcttctt gtttttctag gtcttctttt agggctgcaa ccatgcctac
     6121 gatagcaggc agattttcag ttcctgcacg ttttttctgt tcctggtctc cgccatgtag
     6181 ataggaatca aagtccatgc tagatgcgta gagaaaaccg attcccttag gaccatggaa
     6241 tttgtgggca gaagcagtga gaaaatcaat gcccagttct tctgaatgaa ttgggatttt
     6301 accaatagcc tgaactgcat caacatgata ggcagcaggg tgttgcttga gtatttggcc
     6361 aatttcagcg atgggcagta ggtttcctgt ctcattattg gcaaacatgg tagaaaccaa
     6421 aatcgtatcg tcacgtaaag ccttttgaat ttgctgggct gtgatttctt gattttctgg
     6481 ctggataatg gttgcttcaa acccaaagtg ttgaaccaag taatcaattg tttcaaggac
     6541 agcatggtgc tcgatggcag ttgtgatgat atgttttcct tgttcttggt gacgaagaca
     6601 gtagccaatg atggtagtat tattgccttc agtcccacca gaagtgaaaa agatatgttg
     6661 aggttttgtc cttagtaact gggctagttc ctgacgggct tctcgcaaga gtttgccagc
     6721 ttgacgacca tgaccatgaa tactagaagg atttccgtgg gtttcttgca taaccttggt
     6781 catagctgaa atagcaactg ctgacatagg agtcgttgca gcattgtcca aataaatcaa
     6841 agaatcacct tatttctttt tattgtaggc aaagagtggg ctgactggtt ttctttcgtg
     6901 aatacggacg atagcatcac caattaactc actagcagtg atgtagcata catttttagg
     6961 agttttttct tttgttgcta ctgaatcagt cacaagaatt tctttaatat tagtattgtc
     7021 aagaagctca gcagctccct cgacgaagag accgtggcta gaaacagcat aaatttctgt
     7081 agctccttca cgttcaacga ttttagaagc ttcagagaag gtacgtcctg tatttaaaat
     7141 atcatcaatc aagatagctt tcttaccttc aacatcacca ataatataac cttcgttacg
     7201 agttgcatcg tcttgagggt agtcgataat ggcgatagga gcatcaagat attcagccag
     7261 gctacgcgca cgtttgacac ctgaattttt agggctaacg acaacaacat ctgaaccaag
     7321 caatccttta tcgcagtaat gttttgcgaa taggggaaca gtgaaaagat tatccactgg
     7381 aatatcaaag aaaccttgaa cctgaacggc atgcaaatca agagtcagga tacgatcaac
     7441 tccagcctta accagcatat tggcaactag ttttgctgta agtggctcac gaggacaagc
     7501 aatgcggtct tgacgtgcat agccaaaata tggaaggaca acgttgatac tgtgggcact
     7561 tgcacgcaca caagcatcga ccatgattaa caattccatt aggtggttgt tgacagggaa
     7621 acttgttgat tggatgatgt aaacatcata accacggaca ctttcttcga tatttacttg
     7681 gatttctccg tctgaaaatt gacgtgatga tagttttcca agtgggacac caacagcttg
     7741 ggcaattttt tgtgcaatct cttggttaga gttgagtgcg aaaagtttca tgttttttct
     7801 atctgacatt atagaccgtc ctctgtaaac tttataaatc ctagttatat ttaccttaca
     7861 tatatgaact gggatttgtg tatttttatc ttttctattt taccaaaaaa tggagattat
     7921 ttcagctatt tttcatactt ttgacaaatc gaaccaattt tgaaggagct ttttggtagg
     7981 aaatctgatt tttctctaaa aattgtcgga aatcctgttt gccttgctca tgattttcca
     8041 cttcaagctc caattcgtaa tctgttatat caaagtatcg gctctgatcc agtgccatga
     8101 gaccaatagc tgttttcatt tcatagcgaa gcgttgttag acaaccaaga acctgccagt
     8161 tcttactttg gataccatgt ttcgccaatt catccagtac tagtccttga ggaagttctt
     8221 ccttactcag atagtcctca gcatctttta gttgcaattt ttggttgtat tccatgtttc
     8281 caacactctg cgggactttg agtgtcaact cagcccagtc ttcaaaggtt cgaatgcgca
     8341 tagcgacttt cttttctcgc agttcaaaat caggcgtgtc gatgtagtaa tttgtttgaa
     8401 gaacaggagt gacacctgtg aactggtctt ttagacgatt gtattcatct tttttcaata
     8461 gtgttttcaa ttcaatttct aaatgtttca tttttcttac ctttttttat cgttgaaagc
     8521 ggatttatgg tataataagc attgtattta ttgtatatga atctggagaa aaaatcaaag
     8581 atatttttga cggataatat gagaacaagg gagaatatat gaccttagaa tgggaagaat
     8641 ttctagatcc ttacattcaa gctgttggtg agttaaagat taaacttcgt ggtattcgta
     8701 agcaatatcg taagcaaaat aagcattctc caattgagtt tgtgaccggt cgagtcaagc
     8761 caattgagag catcaaagaa aaaatggctc gtcgtggcat tacttatgcg accttggaac
     8821 acgatttgca ggatattgct ggcttacgtg tgatggttca gtttgtagat gacgtcaagg
     8881 aagtagtgga tattttgcac aagcgtcagg atatgcgaat catacaggag cgagattaca
     8941 ttactcatag aaaagcatca ggctatcgtt cctatcatgt ggtagtagaa tatacggttg
     9001 ataccatcaa tggagctaag actattttgg cagaaattca aattcgtact ttggccatga
     9061 atttctgggc aacgatagaa cattctctca actacaagta ccaaggggat ttcccagatg
     9121 agattaagaa gcgactggaa attacagcta gaatcgccca tcagttggat gaagaaatgg
     9181 gtgaaattcg tgatgatatc caagaagccc aggcactttt tgatcctttg agtagaaaat
     9241 taaatgacgg tgtaggaaac agtgacgata cagatgaaga atacaggtaa acgaattgat
     9301 ctgatagcca atagaaaacc gcagagtcaa agggttttgt atgaattgcg agatcgtttg
     9361 aagagaaatc agtttatact caatgatacc aatccggata ttgtcatttc cattggcggg
     9421 gatggtatgc tcttgtcggc ctttcataag tacgaaaatc agcttgacaa ggtccgcttt
     9481 atcggtcttc atactggaca tttgggcttc tatacagatt atcgtgattt tgagttggac
     9541 aagctagtga ctaatttgca gctagatact ggggcaaggg tttcttaccc tgttctgaat
     9601 gtgaaggtct ttcttgaaaa tggtgaagtt aagattttca gagcactcaa cgaagccagc
     9661 atccgcaggt ctgatcgaac catggtggca gatattgtaa taaatggtgt tccctttgaa
     9721 cgttttcgtg gagacgggct aacagtttcg acaccgactg gtagtactgc ctataacaag
     9781 tctcttggcg gtgctgtttt acaccctacc attgaagctt tgcaattaac ggaaattgcc
     9841 agccttaata atcgtgttta tcgaacactg ggctcttcca ttattgtgcc taagaaggat
     9901 aagattgaac ttattccaac aagaaacgat tatcatacta tttcggttga caatagcgtt
     9961 tattctttcc gtaatattga gcgtattgag tatcaaatcg accatcataa gattcacttt
    10021 gtcgcgactc ctagccatac cagtttctgg aaccgtgtta aggatgcctt tatcggtgag
    10081 gtggatgaat gaggtttgaa tttatcgcag atgaacatgt caaggttaag acctttttaa
    10141 aaaagcacga ggtttctaag ggattgctgg ctaagattaa gtttcgaggt ggagctattc
    10201 tggtcaataa tcaaccgcaa aatgcaacgt atctattgga cgttggagac tacgttacca
    10261 ttgacattcc cgctgaggaa ggctttgaaa ccttggaggc tattgagcat ccattagata
    10321 ttctctatga ggatgaccac tttctagtct tgaataaacc ctatggagtg gcttctattc
    10381 ctagtgtcaa tcactctaac accattgcca attttatcaa gggttactat gtcaagcaaa
    10441 attatgaaaa tcagcaggtt cacattgtta ccagactaga tagggacact tctggcttga
    10501 tgctctttgc caagcacggt tatgcccatg cacgattaga caagcagttg cagaagaaat
    10561 ctatcgagaa acgctacttt gctttggtta agggagatgg acatttggag ccagaagggg
    10621 aaattattgc tccgattgcg cgtgatgaag attccattat taccagacga gtggctaaag
    10681 gcggaaagta tgcccatact tcatacaaga ttgtagcttc ttatggaaat attcacttgg
    10741 tctatattca cctgcacact ggtcgaaccc atcaaatccg agtccatttt tctcatatcg
    10801 gttttccttt gctgggagat gatttgtatg gtggtagtct ggaagatggt attcaacgtc
    10861 aggctctgca ttgccattac ctatcctttt atcatccatt tttagagcaa gacttgcagt
    10921 tagaaagtcc cttgccggat gatttcagta accttattac ccagttatca actaatactc
    10981 tataaaaact gtctcagagt ataattatta tcttaaagga gaaaactcat ggaagttttt
    11041 gaaagtctca aagccaacct tgttggtaaa aatgctcgta tcgttctccc tgaaggggaa
    11101 gagcctcgta ttcttcaagc aacaaaacgc ttagtaaaag aaacagaagt gattcctgtt
    11161 ttgcttggaa atcctgaaaa aattaaaatt tatcttgaaa ttgaaggaat catggatggt
    11221 tatgaggtca tcgaccctca acattatcct caatttgaag aaatggtttc tgccttggtg
    11281 gagcgtcgca agggcaaaat gactgaagaa gatgtacgca aggttttggt tgaagatgtc
    11341 aactactttg gtgtgatgtt ggtttacttg ggcttggttg atggaatggt gtcaggagcg
    11401 attcactcaa cagcttcaac agttcgtcca gctctacaaa tcatcaaaac tcgtccaaat
    11461 gtaactcgta cttcaggagc cttcctcatg gttcgtggta cggaacgtta cctatttgga
    11521 gactgtgcca ttaacatcaa tccagatgca gaagccttgg ctgaaattgc catcaactca
    11581 gcaatcacag ctaagatgtt tggcatcgaa cctaaaattg ccatgttgag ctattctact
    11641 aaaggttcag ggtttggtga aagcgttgat aaggtcgttg aagcaactaa aattgctcac
    11701 gacttgcgtc ctgaccttga aatcgatggt gagttgcaat ttgatgcagc ctttgttcct
    11761 gaaactgcag ctctgaaagc tcctggaagt acggtagctg gtcaagcaaa tgtcttcatc
    11821 ttcccaggta tcgaggccgg aaatatcggt tacaagatgg ctgaacgcct gggtggcttt
    11881 gcggctgtag gacctgtttt gcaaggttta aacaagccag ttaatgatct ttctcgtgga
    11941 tgtaatgcag atgatgttta caagttgacc ctcatcacag cagctcaagc agttcatcaa
    12001 tagtgaaaac tataaagcga tatactatgc tatactgtag ttatgaaact atgtacgaaa
    12061 agcactgcca ttaattcctg agaactaaat tactgattgg tgtcaaaaag gaaaacttcc
    12121 aagcgatgat atcctgtcta tacacgacct atagaaatct gtaatatgta tgtccgtaaa
    12181 acgataattt ccctttttga ttttaaatga gtatgaaaag agaatttttt catgagaaaa
    12241 cgcataatat taagtgtctt gacacctgct attatgcgtt ttctggctct ttgtcaactg
    12301 tagtgggttg aagaaaagct aagctcgaga aaggacaaat tttgtccttt cttttttgat
    12361 attcagagcg gtaaaaatcc gttttttgaa gttttcaaag ttccgaaaac caaaggcatt
    12421 gcgcttgata agtttgatga gattattggt cgcttccaat ttggcgtttg aatagggtag
    12481 ttgaagggcg ttgacgattt tctctttgtc ctttagaaag gttttaaaga cagtctgaaa
    12541 aagaggatga acctgcttta gattgtcctc aatgagtccg aaaaatttct ccggttcctt
    12601 attctgaaag tgaaacagca agagttgata gagctgatag tgatgtttca agtcttgtga
    12661 atagctcaaa agcttgtcta aaatctcttt attggttaaa tgcatacgaa aagtaggacg
    12721 ataaaatcgc ttatcactca gtttacggct atcctgttga atgagtttcc agtagcgctt
    12781 gatagccttg tattcatggg atttcggatg atggcttgtg ttctgctctc aagaacagtt
    12841 atgatattga gcttatcaaa gtcctgagca ataaagctca tctccatctc ccgattgaaa
    12901 cagtcactcc ccggactgtt tcaacgtccc aagacataat ctcaggaaga cgcgaaaaat
    12961 catgctcaaa gtgaaaatca ttgagcttac gaataacagt tgaagttgag atggaaagct
    13021 gatgggcaat atcagtcata gaaatctttt caattaactt ttgagcaatc ttttggttga
    13081 tgatacgagg gatttggtga ttcttcttga cgatagaagt ttcagcgacc atcatttttg
    13141 aacagtgata gcacttgaat cgacgctttc taaggaggat tctagtaggc ataccagttg
    13201 tctcaaggta aggaatctta gacggttttt gaaagtcata tttcttcatt tgacttccgc
    13261 actcagggca agatggggcg tcgtagtcca gtttggcgat gatttccttg tgtgtatcct
    13321 tattgatgat gtctaaaatc tg
//
LOCUS       DAVLXF010000050        13158 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLXF010000050 DAVLXF010000000
VERSION     DAVLXF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.050"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(132..1334)
                     /gene="rpsA"
                     /locus_tag="VYO12_001385"
     CDS             complement(132..1334)
                     /gene="rpsA"
                     /locus_tag="VYO12_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1587983.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1496..1567)
                     /locus_tag="VYO12_001386"
     tRNA            complement(1496..1567)
                     /locus_tag="VYO12_001386"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1533..1535),aa:Gln,seq:ttg)
     gene            complement(1577..1657)
                     /locus_tag="VYO12_001387"
     tRNA            complement(1577..1657)
                     /locus_tag="VYO12_001387"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1621..1623),aa:Tyr,seq:gta)
     gene            complement(1707..1937)
                     /locus_tag="VYO12_001388"
     CDS             complement(1707..1937)
                     /locus_tag="VYO12_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1587984.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2105..2749)
                     /gene="pcp"
                     /locus_tag="VYO12_001389"
     CDS             complement(2105..2749)
                     /gene="pcp"
                     /locus_tag="VYO12_001389"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1587985.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2764..3687)
                     /locus_tag="VYO12_001390"
     CDS             complement(2764..3687)
                     /locus_tag="VYO12_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1587986.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3684..4373)
                     /locus_tag="VYO12_001391"
     CDS             complement(3684..4373)
                     /locus_tag="VYO12_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1587987.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4442..5116)
                     /locus_tag="VYO12_001392"
     CDS             complement(4442..5116)
                     /locus_tag="VYO12_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1587988.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGFAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQIPK
                     RTRKIH"
     gene            complement(5172..6194)
                     /locus_tag="VYO12_001393"
     CDS             complement(5172..6194)
                     /locus_tag="VYO12_001393"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1587989.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGSVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6324..8795)
                     /gene="parC"
                     /locus_tag="VYO12_001394"
     CDS             complement(6324..8795)
                     /gene="parC"
                     /locus_tag="VYO12_001394"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1587990.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(9215..11158)
                     /gene="parE"
                     /locus_tag="VYO12_001395"
     CDS             complement(9215..11158)
                     /gene="parE"
                     /locus_tag="VYO12_001395"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1587991.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KVGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRSDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11294..11935
                     /gene="plsY"
                     /locus_tag="VYO12_001396"
     CDS             11294..11935
                     /gene="plsY"
                     /locus_tag="VYO12_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1587992.1"
                     /translation="MITIILLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(11980..13158)
                     /locus_tag="VYO12_001397"
                     /pseudo
     CDS             complement(11980..13158)
                     /locus_tag="VYO12_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3758 a         2965 c         2309 g         4126 t
ORIGIN      
        1 acttcaaccc actacagttg acaaagagcc cctttctcga gcttagcttt tcttcaaccc
       61 actacagttg acaaagagcc agaaaaatcc cttgtttagt aaacaaagga ttttgtgaat
      121 tttcaatttg attaaagttc gatgtcacca aacaaatcag ccattgaaaa tcctgtttgt
      181 gtttctggaa gttcgaaatc acgcttttct tgacgtcttg gacgacgtgg acgagcagca
      241 cgtttttctt ctttttgtcc ttcttcttgg gctggacgtt cttcaagagc tttaatagaa
      301 agtgacacgc gttctgcatc tgcgttaact tcaagaactt taacttgaac ttcttgacca
      361 actttaagag cttcttttgg attttcaatc cgtttgtgtg aaatttgtga tacgtgaaca
      421 agtccatcga tacctggcaa tacttcaaca aatgcaccga agtcagtcaa acgtttaact
      481 gttccttcta ctacatcacc tttagccaat ttttgctcaa cgccatccca tggtcctggt
      541 actgttgctt taagtgaaag tgatacacgt ccttcttctt cgttaagatc aaggattttc
      601 acttcaattt cttcaccaac agttacaact gattttggtg atacgttacg ttcatgtgac
      661 aattcagtca agtgaaccaa tccgtcaaca ccaccaaggt cgacgaaagc gccgaagctt
      721 gtgatacgag caactttacc agttacaaca tcaccaacag ccaatttacc gaatacttca
      781 gcgcgagctg ctgcagtagc tgcttcaaca acttcacgac gtgaaaggat gaagcggttt
      841 tctttagcgt taacttcttt gattttagta tcaaattctt gacctacaaa acgctcagca
      901 ttacgtacga aacgagtatc caacattgaa gctgggataa atccacgaac accttcaaat
      961 tctactgaaa gtccaccttt aacggcacgc gttcctttaa cagtaacaac ttcttcttcg
     1021 cgaccaacaa gtttgtccca tgctttgcga gcttcaaggc gttttttaga tacaaggtat
     1081 gtaactgtat cagtatcttt accaactact tgacgaagta caagaacatc caatacttct
     1141 cctactttaa caaagtcatt gatatctgca tcacgatcgt ttgtcaattc gcgaagagtc
     1201 aagacacctt caacaccagt tccagagatt gcaacgttag cttgagtcgc atcaactgtc
     1261 aatacttcag cactaacaac atcaccagtc tcaacttgac taacgctatt tagcaaatct
     1321 tcaaattcat tcatctaaaa aatcctccaa caatcaagtt tttcttaaac ttgacatact
     1381 tataattttt ttcctaagca cccgcaagga catgttcata tcattcacgt ctttcaatta
     1441 taaaaataaa ataccctaag atattttgct ttttatcttg attattacct aagaactggg
     1501 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
     1561 taccccatta atgaaatgga gagagaggga ttcgaacccc cgaacccgaa ggagcggatt
     1621 tacagtccgc cgcgtttagc ctcttcgcta tctctcctac aatcaacatg gactattata
     1681 tcatgaaaat ttcaaaaaaa caagacttat tttgctaaat tataattttc aatcaaaatt
     1741 tccacagctt tttccaattt tttataaaaa ctatcgacat tcccattctt tataatggca
     1801 aattgtccgt ctaattcggc aatttctccg ataacttttt taccgatagt caatgtataa
     1861 ccttcaaaac tgtctttacc aacattaact ttggcatccg ctacttgaat ttcgattttt
     1921 ttatctttct tgctcatttc atacctctct tcactttttc tattttacaa gaaaattcta
     1981 aaactagcaa gaaaaaactc tatatcaggc ttctctgata tagagtttta tgtaaacaac
     2041 ctcaattgat ttagcatagt taatagttag taaacaagca taattgacta tcctttttag
     2101 gttatcagtg agtctcaccg cctactaact gcaaatcctt atctttgtag tctacaatag
     2161 cttcaatagc tacctctatg cctcttacaa tatcacatag actcattcct gcagtattcg
     2221 gcttatttac tacttgttcc atcatatatg gaatatgcat gaaacctgct ctcatattag
     2281 ggaatttttt atccactaaa taaagagctt ggtacatcaa atgattgcaa acaaaggttc
     2341 ctgcactatt ggatacaact gccggaagtc cctgtttttt gatagcttgt accatcgctt
     2401 tgataggtaa actactaaaa taggccgatg ctccatcaat acgaatcggt gtatcaattg
     2461 gttgattgcc ttcgttatca ggtatgcgag catcatcttg attaatagcc actcgttcag
     2521 gtgttaagcc ggtcctgccg cctgcttgtc caatacaaag tacagcatct ggttgatatc
     2581 gtaatatttc tgcctctaaa acttctgacg acttataaaa aaccgttgga atttctaccc
     2641 agcgaacttc agccccatta atctcagatg gtaataattt tacagcctcc aaagctggat
     2701 taatcttttc acctccaaaa ggattaaaac ctgtaaccaa tattttcatt ttattttcct
     2761 ttactaaaat gcgagaaagt acattaagaa tatgtgaata acaatcatta ctagagcaac
     2821 acctgcttga gcctttataa cgccattctg atctttcata tccatcaatg ctgctggtag
     2881 agcgttaaaa ttagcagcca ttggggtcaa taaggtccca caataacctg ctgtcatggc
     2941 aagagcacca gccacaattg gattagctcc cagagcaaat acaaagggaa ctccaacacc
     3001 tgctgtaata acggtgaatg ctgcaaaagc atttcccata atcattgtga atagaaccat
     3061 tccaagaaca taggccaaaa ctcctataaa gcgactatct gaaggaacaa taccgctaat
     3121 cagatgagag ataacatcac caacacctgc tacagtaaaa atagccccca aagcccctaa
     3181 taattgagga acaataccac ttgttgaaac ttgctgagtc attcgattat tttctgataa
     3241 cagactctta gggtgactat tggtaatcac aagaacagaa attgtagcaa acaaggcggc
     3301 aaggctaatc gaaatcttgc taaattctgg aatcatttgc gctaagacca acgcaagtat
     3361 tgccatcagc ataactggaa taaaaatttt atttttcaac ctgttagatt caatattggc
     3421 tttcatttca tctaaggatg gcaaggttcc gatacggact tgcttaaaca atgttaacag
     3481 cgataatagg attacaataa taccaatact catatttggc atataggaac cacctataaa
     3541 cgtaatagac aatagagtcc aaaatgcaga tgtcccaagt cgaactgggt ttgttttatc
     3601 tttataacta caataggctg tatggagaaa ttgacaacca atcacaatat aggtcaactc
     3661 taatagttgc tttgccaact ctgtcatttt tgttctcctc ccctagtctt ttttgatatc
     3721 aattttttat caaataaata attgtaaatc cccactacaa taagtgttat aacagcaaca
     3781 ataatagatg tagaagcaat ccctgcataa ttgctttcat agcctaactg atctaatgtt
     3841 ccccctatca agaggactcc cccagcacct acaaacgtat tttgagcaaa gaaatttcca
     3901 aaattttcat tcgcagccgc acgcgctttt attgtctcat cttcaacctc tgttaacttt
     3961 ctacctaatt gagactctgc agctgcttct cccataggtt gaaccaaagg tctgacaaac
     4021 tgagggtgtc ctcctagacg aattgaaaag aaaccagcta actctcgaat aaagaaataa
     4081 actgtataga agtttccaac tgtcagacct ttaatctttc gaatcaaatc gattgatcgt
     4141 tgcttgagtc caaaggtttc tgacagcccc acaagaggca aggtaaccat aaaaatcgtg
     4201 agcactcgct gattgctaaa ttcttttccc aaaatctcca aaaattcaac gagagaaaca
     4261 cctgaaacta aagctgtaac caaaccagct aagactactg ttgcaattgt atcaaatttt
     4321 aaaataaaac ccacaacaat gattgctatt cctattaatc taatccactc catatcaaac
     4381 tcctttatat tcaaaatgac agtattttaa aaattttatc aagatcaata ccattcctta
     4441 tttaatgtat ttttctagtt ctttttggta tttgccattt tttaaaaacc tcgttatatt
     4501 cttttgttgt aacaatatct ttttgcaatt tcattccttt aaagatatat ggatccccct
     4561 taataccaac ttgtgagtat ggttttgaga atggtactac gttacttaca actggagaac
     4621 caccagatga agctgttggc atcaataatg aactatctgt cgaccaagct tgagctttgg
     4681 catatttttc atatctcttc tctaggtcag tggtctcaga aacagcatct tctaacaatt
     4741 tcttatattt atccaaacca ggtttagcta caacatcctt atcttttcct ttcgtaatac
     4801 caaggtgttt catggcaaaa ccagattttg gatccataat attcaagtaa gacgctggat
     4861 cttgatagct tggagcccat cctgtactat tcaaatcata gtctttttga gaaggagcaa
     4921 cattgccgta tttatcattt tccatcaaac catcaataac atttccaata acgtctgtcg
     4981 tcgatgttcg agtcgctata ctgtagccca atgatgctgg atctactgca tagacataag
     5041 aaaatgttgt cggtgcatcg gcttctttat cagtttttcc acaagccact aaaatagctg
     5101 acgtgctcag gaccactcct gctgttaaga gccacttttt ctatttcata aaaaatctcc
     5161 ttcggtttat tttaatctac ttttacaatc caaccttctg gcgcttcaat atcgccaaac
     5221 tgaatacccg tcaattcatt atataattta cgcgtcacag aacctacttc tgtttcacta
     5281 tagaatacat ggaaatcatc accatgttga atacctccaa ttggagaaat aaccgctgct
     5341 gtaccacagg cacctgcctc tacaaaacgg tcaagattat caattggaac atcaccctca
     5401 ataggagtta atcccaagcg gtgttctgcc aaataaagca aggaatactt ggtaatagat
     5461 ggcaagatag atggactcaa tggtgttaca aattcattat cagctgtaat tccaaagaaa
     5521 ttagctgatc cgacttcttc aatctttgta tgagttgatg ggtccagata gataacatct
     5581 gagaaatggc gtgacttggc catttttcct ggtaagagac ttgcagcata gtttccacca
     5641 accttagccg cacctgtacc atttggtgct gcacggtcgt actcatcctg aatcaagaag
     5701 ttggttggga ccaaaccacc tttaaagtaa tttccaactg gcatagcaaa gatggtgaaa
     5761 atgtactctt ctgccggttt taccccgata atatctccga caccaatcaa aagagggcga
     5821 agatataagg ttccacctat tccgtatggt ggtacgtatt cttcattcgc acggacaact
     5881 gctttacaag cttctacaaa catgtctgtc ggaacttgtg gcatcaagag acggtcacat
     5941 gtacgttgca ggcgtttagc attttcatca ggacggaaca gttgaacact gccatcctta
     6001 gtacgataag ctttcaaacc ttcaaatgct tgttgtccat agtgaagact tggagaagac
     6061 tctgaaatat gcaaagttgc atcctctgta agctctcctt gatcccattg tccatttttg
     6121 aaatgagcaa gatagcgata aggtaatttc atataggaaa aaccgaggtt ttcccaatca
     6181 atcgttactg tcatattcca ctccttattc taaaaaccta tttcttatat tctacactat
     6241 ttttctaaaa tagcaagtat attttgtaat tttcagaaaa ttcctccaat aaaaaccatc
     6301 ttagaactga ttcttcattt cacttattta tcttcagtaa ctacttcctg aagataagcg
     6361 tcaaaaactt cttcatctga aatcgtgtca gaaatgaagc ttccattgct agtgcgttct
     6421 gacaagttca agtcttgcaa tcggctttca tagattgttc ctttattgga ttggacaagc
     6481 agagtttggt cgttcacatc cacttccgta ctgaagaaat cgccaacaaa tccttgctct
     6541 gcaactgctc ctgccaagaa gacacgatgc ggtttgtttt tcaactcacg caagacttgt
     6601 aatcctcgtt tggcacggct ggttgctaga atttcctcaa tggaaacacg tttcaagctt
     6661 ccacgctggg tcaagaggta gaaggacgaa gtattacaga taaagccaga ttggaggaca
     6721 tcatcttctt tcaaattcat agccttgaca cctgctgcct tagcaccgac aaccggaacc
     6781 tcttcgatat tgaaacgcag ggcataacca ttttgactaa ccaagacaac atcatctagt
     6841 ttaatcggag ccactgctac aatctgatct gtatcgtctt tgagcttagc atacttgaca
     6901 gacttagatc tataggtccg ccatggagtg aattcttttc gctctacccg tttgatttga
     6961 ccaaggcgag tcgctgcaaa gtaggttgtc gcatcgtcaa actgatccag tacttccaca
     7021 taaaggattt cttcattcgt ttcaaagttt gtgatggttt ggctcagatg ctctccgatg
     7081 tccttccaac gaatatctgc caactcatgg attggtctgt agatgacatt tccaagactt
     7141 gtgaacatca agaggtgctg ggttgtcttg gcagattgaa caaaaatcaa acggtcatca
     7201 tcacgcttgc caatttcttc caaggtggaa gccgcaaagg aacgtggact ggtacgcttg
     7261 atgtaacctg ccttggtcac gctgacgtag gtatcttcct cagcgataag actagctgta
     7321 tcaatctcaa ttgctttcgc agtgtcttct aaagaactca aacgaggagt tgcaaatttc
     7381 ttcttgacct cacgaagttc tttcttcatg agattgtaca tagtcctttc atcaccaata
     7441 atagccgcca gcatagcaat cttctcacga agctctgctt cttcttcctg caagacaacc
     7501 acatcggtat tggtcaaacg gtacagttgc aaagttacga tagcctcagc ctgttcttcc
     7561 gtaaaatcat agctaacttt gaggttttcc ttggcgtccg ccttattctc agaagcacgg
     7621 ataagagcaa tgacttcatc caaaatcgaa atcacacgaa tcaaaccttc gacgatatgg
     7681 agacgtttct cagccttttc tttgtcaaag cgtgaacgcg ccaaaatcac ttctcgacgg
     7741 tgagcgatgt agctagacag gattggaaca atcccaacct gacgaggtgt gaaattgtca
     7801 atcgccacca tattaaagtt gtagttgatt tgtaggtcgg tgtacttaaa taagtagttg
     7861 agaacaagct cagtattagc gtctttctta agttcgatag cgatacgaag accatcacgg
     7921 tcagactcat cacgaacctc agcaatccca gctaccttgt tattaacacg aacatcatcg
     7981 attttcttga ctagattggc cttattgatt tcataaggaa tctcagtaat aacgatttgt
     8041 tccttaccac cttttagctt ttcaatttca gtcttggaac gaacaaccac gcgccctttc
     8101 ccagtctcat aagctttctt gatttcatca cgaccctgaa taatagcccc tgtagggaag
     8161 tctggtccag gcaagaattc catgagttta tcaatctttg cagttgggtg gtcaatcatg
     8221 taaactgcag catctatgac ctcagctaaa ttatggggag gaatgtctgt ggcataacca
     8281 gccgaaatcc cagtcgaacc attgaccaag aggtttggaa aggctgctgg caagaccgtt
     8341 ggttctttct ccgtatcgtc aaagttccat gcaaaaggaa ctgtcttttt ctcgatatcc
     8401 tgaagaaggt agcctgcaat ttcagacaaa cgtgcctcag tataacgcat agccgcagga
     8461 ggatctccgt ccatagaacc gttattaccg tgcatttcaa ctagaatctc acgatttttc
     8521 cagttctgtg acatacgaac catggcatca tagatagaag aatccccgtg tgggtggaaa
     8581 ttccccatga tgttcccgac tgacttggcc gacttacggt agctcttgtc aaaagtattg
     8641 ctatccttat tcatagaata aagaatacgg cgctgaaccg gcttcaaccc atcacgaata
     8701 tctggcaaag cccggtcttg aataatgtac ttggagtagc gaccaaagcg ctctcccatg
     8761 atgtcctcca gggacatgtt ttgaatgtta gacataagat acaaagccca taaaatacca
     8821 agtgaaaata gaaaattctt gaagtaagca aactcacaag agaatttatc tttttcacac
     8881 aatatctagg gcgtgttcaa ctcctttcaa agaatgtaga gtaggttttt atgcagtaaa
     8941 agatatttta cgggaattcg tcccgtgttc agttacgata agtaaccaaa ctatcctgtt
     9001 tgtatttttc aatatgaaaa tctggttttc caaaattagt cttagtttgt gtcttagccg
     9061 ctcccttaaa cgcctctttg agataagcac tcatagaaga ttcttcatta ataattctgc
     9121 aattttttca aaccaagatt ttcaaactgc tttttcacat agtcattcac atccgactct
     9181 aatttccagt ttactaacat attattttct ttcattaaaa cactgtcgct tcttctagcg
     9241 taaacttgac attatcttca atccatttac ggcgtggttc taccttatct cccatgagaa
     9301 cattgacgcg acgttcggcg cgcgctaaat cttcaattgt gacacggatg agggtacgtg
     9361 tttctgggtt catggttgtt tcccagagct ggtccgcatt catctcacca agtcctttgt
     9421 atcgttggag ggtagcgcct ttaccgaact gtttacgtag ttcttctagt tctccgtccg
     9481 tccaagcgta ggccacttct tctttcttgc ctttaccttt ggacatcttg taaagaggtg
     9541 ggagggcaat atagacatga cctgcctcga ctagcggacg catgtaacgg tagaaaaatg
     9601 tcaagagcaa ggtctggata tgggcaccgt cggtatccgc atcggtcatg ataatgatct
     9661 tatcatagtt ggcatcttca atagagaagt ctgctccaac acccgcacca atggtataaa
     9721 tcatggtatt gatctcttca tttttgagga tatccgccat cttggccttg gctgtattga
     9781 taaccttacc acgaagaggt agaatagcct ggaacttgcg gtcacgacct tgtttggcag
     9841 aaccaccggc agagtccccc tcaactagat agagttcatt cttagcagga ttcttagatt
     9901 gggctggggt caatttccca gacaacaagc ccttatcttt cttgtttttc ttcccatttc
     9961 ggctctcatc acgcgcctta cgtgctgctt cacgagcatc acgggccttg atagccttgc
    10021 ggatgaggtt agaagctaat tccccatttt ccataaggaa aaaggtcaac ttatcagcca
    10081 ctattccatc cacaactggg cgagctaagg ggcttcctag tttatccttg gtctgtcctt
    10141 caaactgcaa gtgttcttca ggaactaaga tagaaagaac ggccgctagt ccctcacgat
    10201 agtctgaacc ttcaaggttt ttatcttttt ccttgagaag acctgtttta cgtgcatagt
    10261 cattcatgac cttggtaatg gcagacttga gtcctgtctc gtgcgttcca ccgtccttgg
    10321 tgcgaacgtt attgacaaag gatagaatgt tatctgagaa tccgtcattg tactggaggg
    10381 ctacttccac ttgaaaacca ttgtcttccc cttcaaagta aagaactggc gtcaagattt
    10441 ccttatcttc gttgagataa gaaacaaaat cttgtactcc attctcatag tggaactcaa
    10501 tcgcttcatc tgatcgcttg tccgttaaag acaaggtcac atttttcaag agaaaggctg
    10561 attcattaag gcgctctgaa atggtattgt acttgaaatc tgtcgtagaa aagatagtcg
    10621 cgtcaggcat aaaagtaact ttggtgcctg ttttagactt gggtgctgta ccgactttct
    10681 tcaaagtcgt gacaggtttt ccaccatttt caaaacgttg cttgtaaact gcgccatcac
    10741 gggtaatttc aacttctaac cagctagaaa gggcgttaac aacggaagaa cccactccgt
    10801 gaagtccacc tgatgtctta tagccacctt gaccgaattt ccctccggca tgaagaatgg
    10861 taaagataac ctcaacagtt ggaattccca tagcgtgcat acctgtcggc atcccacgtc
    10921 catggtcttg aaccgttaga ctaccgtctt tattgatagt tacatcaata cgatcaccaa
    10981 acccagacaa ggcttcatcg actgcattat caacgatttc ccaaactagg tgatgaagac
    11041 cagcgccatc ggtcgatcca atatacatcc ctggacgttt tcggaccgca tccaaccctt
    11101 ctagcacctg aatagcatca tcattataat tgttaatatt gatttccttt tttgacacaa
    11161 ggaacctcct attcgttcat ctttactatt ctacaggttt tccaaggatt ttgcaaaatt
    11221 tttctttctc cgatgtgaca atttcagcag agattctctg cttttctttc ccaattcatg
    11281 atataatagg agtatgatta caataatttt attaatccta gcctatctgc tgggttcgat
    11341 tccatctggt ctctggattg gacaagtatt ctttcaaatc aatctacgcg agcatggttc
    11401 tggtaacact ggaacgacca ataccttccg cattttaggt aagaaagctg gtatggcaac
    11461 ctttgtgatt gactttttca aaggaaccct agcaacgctg cttccgatta tttttcatct
    11521 acaaggcgtt tctcctctca tctttggact tttggctgtt atcggccata ccttccctat
    11581 ctttgcagga tttaaaggtg gtaaggctgt cgcaaccagt gctggagtga ttttcggatt
    11641 tgcgcctatc ttctgtctct accttgcgat tatcttcttt ggagctctct atcttggcag
    11701 tatgatttca ctgtctagtg tcacagcatc gatcgcggct gttatcgggg ttctgctctt
    11761 tccacttttt ggttttatcc tgagtaacta tgactctctc ttcatcgcta ttatcttagc
    11821 acttgctagt ttgattatca ttcgtcataa ggacaatata gctcgtatca aaaataaaac
    11881 tgaaaatttg gtcccttggg gattgaacct aacccatcaa gatcctaaaa aataaaatgc
    11941 cagttctgtg aactgccccc caaaagttag acaaataatt tatccaaagg atttagttct
    12001 gtactgcaca ggactaagtc cttttagttt taccttaatt cgtttattgt tgtagtaatc
    12061 aacatagtct acaatagctt gttccaattg cttaagcgac tgaaacgact tctcataacc
    12121 gtaaaacatt tccgatttca gaatcccaaa gaaggactcc atcataccat tgtctgggct
    12181 gttaccctta cgtgacatag atgcttgaat tcccttactc tctaggaacc gatgataaga
    12241 atcgtgtcgg tattgccagc cttggtcact atggagaatc gtattctcat agtgcttctc
    12301 tgtgaatacc tgttccaaca ttgtttgtac ttgttctaag ttgggtgaag ttgaaagatt
    12361 ataggcgata atttcgctat taaagccatc taaaactggt gataagtaaa gcttttgagt
    12421 acttgctgga atggcaaatt ctgtcacatc tgtgtagcac ttttccattg ttttagagcc
    12481 ttcaaattgg ccttgaatga gattctctgc tttcttacca acgtctcctt tatgagaaga
    12541 atattttcgt ttctgtcgca ttttagcttg taaattgagt acttttatca agccttgaac
    12601 tcttttatga tttaccagat aagcacgatt tcttagttct aaatgaaccc ggcgataagc
    12661 ataatttcct ttgtgttcga taaagatgga ttgaatttca gctttaagct cttggtcctt
    12721 atctgttttg tctagctgtt tcaagtgata gtagtaggtc caacgagcta gtttaatgga
    12781 ttttagaaga agaactaacg aaaactcagt cattaattct tgaacaattt ctgtccttct
    12841 tctttctctt tttcctcctt caatcggagt tctcttaact tttttaggat ggcattctcc
    12901 gctctcaggt actcattttc tgcttgaaga tattctaatt ctgtcctctc ttcaggtctc
    12961 gtttttggct tacgtcccat tttaggtact ctccctcttg ttttctcaac aatagtatac
    13021 ccgtttttcc tgtattgtgc tagccagtta agaagtatcg tacgacttgg gaggccgtat
    13081 tcaagagaaa ctctatcttt agtccagcct tcatgtcaga ctttattaat catttcttgt
    13141 tttaaatcag gagaatag
//
LOCUS       DAVLXF010000051        13142 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLXF010000051 DAVLXF010000000
VERSION     DAVLXF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.051"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            466..759
                     /locus_tag="VYO12_001398"
                     /pseudo
     CDS             466..759
                     /locus_tag="VYO12_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            866..1217
                     /locus_tag="VYO12_001399"
                     /pseudo
     CDS             866..1217
                     /locus_tag="VYO12_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1493..2799
                     /locus_tag="VYO12_001400"
                     /pseudo
     CDS             1493..2799
                     /locus_tag="VYO12_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2839..3849)
                     /gene="tsaD"
                     /locus_tag="VYO12_001401"
     CDS             complement(2839..3849)
                     /gene="tsaD"
                     /locus_tag="VYO12_001401"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1587993.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3839..4276)
                     /gene="rimI"
                     /locus_tag="VYO12_001402"
     CDS             complement(3839..4276)
                     /gene="rimI"
                     /locus_tag="VYO12_001402"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1587994.1"
                     /translation="MIEIKRIQQQPALAQDIYAVMAAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLTVQETLFEAEVLQIAVKGACQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4273..4956)
                     /gene="tsaB"
                     /locus_tag="VYO12_001403"
     CDS             complement(4273..4956)
                     /gene="tsaB"
                     /locus_tag="VYO12_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1587995.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5134..5319
                     /gene="cibA"
                     /locus_tag="VYO12_001404"
     CDS             5134..5319
                     /gene="cibA"
                     /locus_tag="VYO12_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1587996.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5321..5473
                     /gene="cibB"
                     /locus_tag="VYO12_001405"
     CDS             5321..5473
                     /gene="cibB"
                     /locus_tag="VYO12_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1587997.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            6291..6524
                     /locus_tag="VYO12_001406"
     CDS             6291..6524
                     /locus_tag="VYO12_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1587998.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6526..8205
                     /locus_tag="VYO12_001407"
     CDS             6526..8205
                     /locus_tag="VYO12_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1587999.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8400..10313)
                     /gene="mnmG"
                     /locus_tag="VYO12_001408"
     CDS             complement(8400..10313)
                     /gene="mnmG"
                     /locus_tag="VYO12_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1588000.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWASFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10323..10778)
                     /locus_tag="VYO12_001409"
     CDS             complement(10323..10778)
                     /locus_tag="VYO12_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1588001.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(10919..12040)
                     /gene="mnmA"
                     /locus_tag="VYO12_001410"
     CDS             complement(10919..12040)
                     /gene="mnmA"
                     /locus_tag="VYO12_001410"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1588002.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            complement(12441..>13142)
                     /locus_tag="VYO12_001411"
     CDS             complement(12441..>13142)
                     /locus_tag="VYO12_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1588003.1"
                     /translation="PAPKPAPAPKPETPKTGWKQENGMWYFYNTDGSMATGWLQNNGS
                     WYYLNANGAMATGWAKVNGLWYYLNSNGAMATGWLQYNDSWYYLNTNGAMATGWLEYN
                     GSWYYLNANGAMATGWLEYNGSWYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAK
                     VNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKANQWFKVSDKWYYVNGLGALAVN
                     TTVDGYTVNENGEWV"
BASE COUNT         3733 a         3063 c         2597 g         3749 t
ORIGIN      
        1 tataatcatc aataaccttt gaatctctag atacgattat caaaatagga tatagagaca
       61 tccttctgat ctgcttttta caaagtccaa ttatatgcgg atctatacct ccacaatgtc
      121 cattattata cctaactata atatgagccg aaaacactat atccttaatg tctccatatc
      181 catcagggat attaatattt atttttccac aactatattg cattgtaacc atctccttaa
      241 acgacgcatt atgatatttg atagagaaat ttttatgaat aactcaataa ttttatagta
      301 aatcatgctt atatctcaaa gatacctatt ttatcttgtc tcgaccttct ccaaagaatt
      361 gctataatac tattacaaat ccatctgcac tacacttcaa attttagcac tgtataaaaa
      421 cgtttcaata cactaacttc aagaaaactt ccactattaa ttgaaaaaat tgatagagat
      481 aaattaaaaa tctatattga aactcatccc gatgcttatt tgactgaaat agctgctgaa
      541 ttcaactgtc ctccaacaac tattcattac gctctaaagg ctatgggata tagtctaaaa
      601 aagagccgta cctactgcga acaagaccca gaaaaagtaa atcggttcct taaagaattg
      661 aatcacttaa gctacctgac tcctatttat atttatgaga caggggttga gacctatttt
      721 tatctcgaat atgatcgagc cttgagcagg cagttagtct ctctggaaga agatataatt
      781 atttgaatta agatcgagac aacgcacacc agagattgcg atactgttat agaagtacta
      841 atgccctttt ttgtttcaat atactatggc tccgatgacc tataaagata cgatgacgag
      901 tgactttttc gaagcttgct tccaaaaatt cttactacct actttagata caccatccct
      961 tatcattatg gaccatgcaa ggtttcacag aatgaacatg tgtaaggagc agggcataga
     1021 ctgttaccac ttcctaccta ttcacccgag tataatccca ttgagaaaat atgggcttac
     1081 atcaaaaaca tctcagaata atattgtcaa attacgatgc ttttcttgag gcacttttgt
     1141 cctattcttg tttcagccga ctatactccg ttattgggca gctacggaac agtcgatggg
     1201 acgatggggg gacataaaaa aatcctccag ttttgttttt tataacagta tactggagaa
     1261 ttgacaatct cggtagatac ctcgttatag cgcggttact tattaggcag ttacaaaaca
     1321 actgtgaaca gaaaacattc cagagtcaga caagactttg gaatgctttg gctctataat
     1381 ttctgtagtg ggtaatccca ccccaggaat tatagggtcg tttcttgtag aaaaaaagcc
     1441 ccatatgacc tataatgaaa agcgtctaac caactcatta gaaagggttc atatggaaca
     1501 acttaagaat accacagatt tgctcggatt ggaagacaaa aatatcaaaa tcttgtctgt
     1561 tctgaaatac caaacccatc tagtcgttca ggcaaagttg gattcccccg ctcctccttg
     1621 tcctcattgt caagggaaga tgatcaaata cgacttccag aaagcctcta aaattccgct
     1681 tctcgactgt cagggtttac ccacggtact gcatctcaaa aagcgccgct ttcagtgcaa
     1741 gaattgcctt aaggtggtcg tttctcaaac atccattgtc aagaaaaatt gccagatttc
     1801 caacatggtg agacaaaaaa tcgctcagct cctccttgaa aagcagtcta tgactgagat
     1861 tgcccacaga ttggcggtct caacttccac cgtcatccga aaactgaggg aatttaagtt
     1921 tgaaaccgat tggaccaagt tgccaaaagt tatgagttgg gatgagtata gcttcaaaaa
     1981 gagcaaaatg agcttcattg cccaagattt tgagtccaaa tccatcctcg caattttaga
     2041 cgggcgaact catgcggtga ttcgaaacca tttccaacgc tatcagagag aggttcggga
     2101 gctggtcgag gtcatcacca tggacatgta cagcccttat tatcggctcg ctaagcaact
     2161 ctttccaaag gcgaagattg ttcttgaccg cttccacatt gtccaacatc tgagccgagc
     2221 tatgaaccga gtacgaatcc aaatcatgaa ccaatttgac cgaaaatcct tggagtatcg
     2281 ggcgctcaag cgcttttgga accctcgctt tttcgtttct aggctcgggc taaatcagtc
     2341 cactggactg atttactaca ccagtatagc ttcaagctct gtcagaaacg attctatcag
     2401 cccacgtttc gaatgcactt aacccatcgg gaagtacgag ataagctgct ttcttactct
     2461 gagggattac aggttcacta cgaactctat caactcctgc tctttcattt tcaagagaag
     2521 aatgccgacc atttctttgg attgattgag caagaactgc caacggttca tccgcttttt
     2581 caaacggtct tttggacttt tttaagggat agagataaga ttatcaacgc acttaagctg
     2641 ccttattcca acgctaaact tgaagcgacc aataatttga ttaagattat caagcgcaaa
     2701 gcctttggtt tccggaactt taacaatttt aaaaaacgga ttttgatgac tttgaacatc
     2761 aaaaaagaga gtacgaattt cgtactctcc agattgtagc ttttcgccta cccactacac
     2821 ttgacaaaga gccactcttt attccatggt atcaaaggca agacttggtt tggcattgag
     2881 gtcccagcct gcgaagtttt ctttgttcca ctcgctgacg ctggcatagg caatcatacc
     2941 tgcattgtct ccgcagagtc gcagaggggg gatgataacc ttgacatctg tgatttcggc
     3001 tgctaggcgt tctctgagac ctttattggc tgccacacca cctgccacaa ccagggtttt
     3061 aacaggatat ttctccaaag ccttcttggt ttttgccatg agaatgtcca taactgctgc
     3121 ttggaaggaa gcacacaaat cttctgtaga caggctttct cccttttgct cggcattgtg
     3181 ataaagattg ataaaggcag atttcaaacc tgagaaggag aactccagat tatcttcctt
     3241 aatcatggca cgggggaaat cataaatatc ctgcccctga tgagccagct cgtcaatctc
     3301 acgacctgca ggataggtca agcccatgac acggccgacc ttatcataag cctcaccaac
     3361 cgcatcatca cgggtttccc caacaatctt ataatctcct gcctccgaaa cataaaccaa
     3421 ctctgtgtgc ccaccgctaa ccaagagggc tagcaaggga aactccaaag gttccacact
     3481 ttgagccgcc atgagatggc cagccatgtg gttaacggga atcagcggaa gtccatgtgc
     3541 ccaagcaaag gccttggcag ctgacaaacc aactagtaag gctccgacca agcccggtcc
     3601 ataggtaacc gccacagctg tcacgtcctc ttctgtaatc cctgcttctg ctagcgcctc
     3661 ctcgatacag gctgtaatga cctcgacatg gtgacgactg gctacttctg gcactacgcc
     3721 cccaaaacgt ttgtgactct caatttgact agcaatgaca ttggacaaga gctcatcgtc
     3781 gtttttcaag acggcaacac tggtctcatc acaggatgtc tcaaatgcta aaatatatct
     3841 atccttcatc tatttctctc ttcatgataa tggcgtcctc gactgggtca tggtagtagg
     3901 cttttcgctc agcgatgacc gccatttttt ctttcttgta aaatgcttgc gctcgttgat
     3961 ttgactgtct aacttcgagg aaaatttcct tgtctgtcgg caattgagca aacaaggctg
     4021 acgcaattcc ctgaccctga caggctcctt tgacagcgat ttgcaggact tctgcttcaa
     4081 agagagtctc ctgaacagtt agaaagccaa tcacttctgc cccatcatag gccagagcat
     4141 accaagtctg gtcttgggac agatctgctt ggatttgttc tagtgtccaa ggactgactg
     4201 ggtaaacagc tgccatgaca gcgtagatat cttgagccaa ggcaggttgc tgttggatac
     4261 gtttgatttc tatcataggc gtttaatgta agactcacca gactcggtat ggttcttgag
     4321 ccagttttcc tctgcctcaa cacgtttgag gtaatttggc acaaaatcat gcaaggagtc
     4381 tgcttccttg tcccaggcca aaagagctag attagctgca ttgggcaatg tttctttgta
     4441 atcagtcctt ggcaagtgtt tttgaatctg ctcaacaaag gggccaactt ctccgacaaa
     4501 ggttacctga ctagcgccct tgattagctc aatgactcgc tcaaaaggca gatgtgcttc
     4561 tgccatgaca ggtttggcat tttcataaaa tcctgcataa acattattgc gacgcgcatc
     4621 catcaaagga acaaacaaac cttcttgttg atatggcact agagccaaga gactagacat
     4681 accaaccaac tcaatgttca gggtgtaagc taaggtctta gcagttgcta ccgcaattcg
     4741 caagcctgta tagctacccg gcccttcagc taccacgatt cggtccaaat ccttgggtgt
     4801 ccaatccaaa cttgccatca aaaaatcgat ggcaggcata agagtaatac tgtgattttt
     4861 cttaatatta atcgtcgtct cggcaagaac ctgcttatcc tctaaaatag ccagagaaag
     4921 agccttgctg gacgtatcaa aagctaatac tttcataaca cattcctatc tttttgtctg
     4981 cttactatta tactacaaaa gctggcacat gggaattttc tttgccccca gacaagagtg
     5041 ccctcactta actaaaaata atttaaaaaa atgctcactt ttccttttct tttccgaata
     5101 taaaagtgaa caagaaaaaa ggaggaaagt tcaatgacaa attttgacat tcttgacaat
     5161 caatttttat ccttatctga aaatgaatta tcagatattg atggcggtct cgctcccttg
     5221 gttatctttg gagtagcagt atcttggaag gctattgcag gtggaacagc acttataggt
     5281 tctggtttgg cagctggtta ttttttagga ggagattaat atgatgaaag atttgaacaa
     5341 ttatcgtgaa atttctaata aggaattgca agaaatcaag ggtggctttg gtgtcggtgt
     5401 tggtatcgct ttatttatgg caggttatac cattggaaaa gaccttcgta aaaagtttgg
     5461 taagtcatgc tagataagaa acacattttt agaaggataa attttattgt cttcatctct
     5521 tacagtttgc tcagcattct caatgatttg aacattacta ccatcccttt accattcgat
     5581 ttatctgttt gtattgtttt atttttatgc ttcaactcta tttttgagta gaacaatgac
     5641 tcccataaaa ataataagct ttgaaaattc cattgtcatg tcatgttaga aaaatgcaaa
     5701 gaccacctca tcttgataga tggggtggaa ttttcgtgtc gtaaatctac tatctctaca
     5761 ttcccaaaca aaaaaaacca gcataagcag ggcatctaag catttaatcc aaagtaaaat
     5821 acaaaccaaa cgacataggt cacgaggagg agaaaaagcg agtagagagt cacaaaggta
     5881 attttccaca agaacttggt ttgtcatcgt tccagtttgg caaatagaag atttcctcca
     5941 taaacgcaag caacaaaaac gataaaagct agcaaacgaa ctccgatagc aaaagcaaat
     6001 aagttataca tagggcaacc tccttgactt aaaatctata tggaattatg acaagtaata
     6061 aacttcactt ccgttatcaa cataacacat tttctttatt tttgcaaacg attaccaaag
     6121 aaatcgcccc ctaactttct cgtttccgtc ttttactaat ttttcatttt gtggtataat
     6181 tgaaataatt gtaacgaatc aaggtcaatc tagacacaaa atggaatgaa atcaagcaaa
     6241 tatctgctaa aagtttggaa taagctgacc tgtaaataga aaggaactat atgatttaca
     6301 aagtttttta tcaagaaaca aaagaacgta gcccacgccg tgaaacaaca cgcgcgcttt
     6361 acctagacat cgatgccagc tcagaacttg agggtcgcat cactgctcgc caacttgtcg
     6421 aagaaaatcg cccagagtac aatatcgaat atatcgaact cttgtctgac aaattgctcg
     6481 attacgaaaa agaaactggc gccttcgaaa tcacggagtt ctaatatggc ctacactctt
     6541 aaacctgaag aagtcggcgt ttttgccatc ggtggtctag gagaaatcgg gaaaaacact
     6601 tacggaattg aataccaaga cgagattatc atcgtcgatg ctgggattaa attcccagaa
     6661 gatgacttgc ttggtatcga ctatgtcatt cctgactact cttacatcgt agacaatatc
     6721 gaccgcgtca aggctgtttt aatcacacac ggacacgagg accacattgg tgggattccg
     6781 ttcctactca agcaagcaaa tgtccctatt tatgctggac cgcttgcctt ggctttgatc
     6841 cgtgggaaac tcgaagaaca cggcctcttg cgcaacgcca aactttacga aatcaaccac
     6901 aacaccgagt tgacctttaa aaatctcaag gcaactttct ttagaacgac tcactctatt
     6961 ccagagcctt tggggattgt cattcatact cctcaaggga aaatcgtctg tacgggtgac
     7021 tttaagttcg actttactcc agttggagaa cctgcggact tgcatcgtat ggctgcgctt
     7081 ggtgaagaag gcgtgctctg tctcctgtct gactcgacaa atgcggaagt accaaccttt
     7141 accaactctg aaaaagtcgt tggtcagtcc attatgaaga ttatccaagg tattgaagga
     7201 cgtatcatct ttgcatcctt tgcctcaaat atcttccgtc tccagcaggc aacagaagct
     7261 gctgttaaga ctggacgcaa gattgcggtc tttggtcgtt ctatggaaaa ggccattgtc
     7321 aacggaatcg atcttggcta catcaaagct cctaagggaa cctttatcga gccaaatgaa
     7381 atcaaagatt atcctgcagg agaagttctt atcctctgta caggtagtca gggtgagcct
     7441 atggcagccc tctctcgtat cgccaacgga acccaccgtc aagtacaatt acaaccaggt
     7501 gataccgtta tcttctcttc tagtcccatc cctggaaaca ctactagcgt caacaagctg
     7561 attaacatca tttctgaagc tggtgtcgaa gttatccacg gtaaagtgaa caatatccat
     7621 acatctggac acggtggtca gcaagagcaa aaactcatgc tctgcttgat taagccaaaa
     7681 tacttcatgc ctgtccacgg tgaataccgc atgcaaaaag tccacgctgg actagcagtg
     7741 gatactggtg ttgagaagga caatatcttt atcatgagca atggcgatgt gcttgccctt
     7801 actgctgact cagctcgtat cgcaggtcat ttcaacgccc aagatatcta tgtcgatgga
     7861 aatcgtatcg gtgaaattgg cgcagctgtc ctcaaagatc gtcgcgatct atctgaagac
     7921 ggtgtcgttc tggcagtcgc aactgttgac ttcaaatcgc agatgattct atctggtcca
     7981 gacatcctca gccgaggctt tgtctacatg agagagtctg gcgacttgat tcgccaaagc
     8041 cagcgtatcc tcttcaatgc cattcgtatc gcactgaaaa ataaggatgc tagcgtgcaa
     8101 tctgtcaatg gtgccattgt caacgctatt cgccccttcc tctatgaaaa taccgaacgt
     8161 gaaccgatca tcatcccgat gatcctcaca ccagatgaag aataaagcaa gaaaacagcc
     8221 ccgtcttcgg agctgttttt ctctatgctt tcttttgaga ttaaaactca atgaaaatca
     8281 gaccagaagg ctaggaaaat gcataacttg agttagctcc catagttcgg ggaactatgg
     8341 gaggttggag atgaatcaaa gccaagcttt gaactcattc gtaagaagcc gacgacgtat
     8401 cattttgatt tttgaagagt tttagaaata ctacgatttt taccttccag atacaccatc
     8461 aaaatagaaa tatctgctgg gtttactccc gaaatacggc tggcttggcc gatggtttct
     8521 ggattgatga gtttgaactt ctgacgagct tccgtcgcaa tagaatcgat gtcatcccag
     8581 tcgatattgg ctggaatgcg tttttcttcc atacgtttca tcttggcaac ctgatccatg
     8641 gctttggaaa tatagccttc gtacttgatt tctgtttcaa tcaattcgat aatcttgtca
     8701 tccaagtctt ctgcagctgg tccgatgaag gccaccacat cttggtaaga aacttctgga
     8761 cggcgaagga attccttggc tgtcaccgca tctgtcaacg gcttgaagcc catttcctca
     8821 accttagcat tggtttcctt gactggcttg agtttgatac tgtctaggcg tttcatctca
     8881 ttatcaaatt gatttttctt gatttcaaaa ctagcccagc gttcatcatc cacaaggcca
     8941 atctcgcgtc ccatctcagt caagcgcata tcagcattgt catgacgaag aatgagacgg
     9001 tattcagcac gacttgtcaa gagacggtag ggttcaatgg ttcccttggt caccaagtcg
     9061 tcgatcatca ccccgatata accgtcactg cgcttcaaaa tcaactcagg cttgccttgg
     9121 attttcagag ccgcattgat acccgcgata atcccttggc cagcagcttc ttcataacct
     9181 gatgttccat ttgtctgacc agcagtgaag agacctgaga ttttcttggt ttccaaagtc
     9241 gcacgcaact gatgaggcaa gaccatatca tactcaatag cataacctgt ccgcatcatc
     9301 tctgcatttt ccaaaccttt gatggaatga accaagtcac gctggacatc ctcaggcaga
     9361 ctggttgaaa gtccttgaac atagacttcc tcagtattgc gcccttctgg ctcaaggaag
     9421 agttggtgac gttccttgtc cgcaaagcgc acaatcttgt cttcaatcga cggacagtaa
     9481 cgaggcccca ctcccttgac cacacctgta aacataggcg cacggtggag gttgttttgg
     9541 ataatctcat gactggtacc attggtgtag gtcaaccagc atggtacttg gtccttgaca
     9601 taatcctcat cacgtgaagt gtatgagaag tgattaggtg cttcatctcc tggttggatt
     9661 tctgtcacgt cgtagttgat agaagaggcc ttgacacgtg gaggggttcc tgtcttgaaa
     9721 cgaccgattt cgagacccag ttccttgaga ttgtcagcta ggttaataga agccaagctg
     9781 tggttaggac ctgatgagta cttgaggtct ccgatgataa tttccccacg gagagcagtc
     9841 cctgtcgtca caataacagc cttagcagca tattcttgat gggtggctgt acgcacaccg
     9901 acaaccttac catcttccac caaaatctca tcaatcatgg tttgacgaag ggtcagattt
     9961 tcttgatttt caactgtctt gcgcatttcc ttagagtaaa gttccttgtc agcctgcgca
    10021 cgaagagcac gaacagctgg ccccttccct gtgttgagca tcttcatctg gatgtaagtc
    10081 ttgtcaatag tcttggccat ctcgccaccg agggcatcga cttcacgcac gacaatcccc
    10141 ttggcagaac caccgataga gggattacaa ggcatgaaag ccagcatttc aatattgatg
    10201 gtcgcaagca ggaccttaca gcccatacgg ctagcggcca aggaagcctc aaccccagcg
    10261 tgtcccgcac caattacaat aatatcgtat tcttcagtaa aatgataagt catgtttctc
    10321 tcctattcct caagatgaat gtgtcttagt tggccttccc aatctggtag ggctgttttt
    10381 aaaaaggctg gaactagctg gatatcctca agcttatcca agtcaatcca ctcacagggc
    10441 tgccttttct catcttcctg catggtcaac ggggcatctt caagcaaatc caccagataa
    10501 tgaaactcga tattgtgata ggaaacgccg tccacttcaa aacgattttc aaccacaaaa
    10561 gctagctgcc cagcttgagc tttaacaccc agttcttcct tcacttcacg gactaccgcg
    10621 tcttccgttt tttcgttaac ttgaatcgca ccgccgataa tgtaatactt gcccttgtct
    10681 ttggtgacta gaagcttgtg attttggaca atcaaggctg tcgcccgaac accaaaaacc
    10741 gtattgtcta cttttgtccg aaagtcttgt tgagtcattc ttgtcctttc ccttaaacga
    10801 cacaaaaaca gtcaaaactc caaagaagtg caggacaaaa aagcctgcaa catccatgag
    10861 ctttgaccat catttctatt gctgttatta ttgtagcaaa ttgagaaaat ttgtcaatct
    10921 aaatgtactg acaaacttgt ccatcgcggt aagcattgtc aatcaaaccg ccacctagac
    10981 actcttcgcc atcgtaaaag acaactgcct gtcctggtgt aatcgcgcgt tgtggttccg
    11041 caaagataac ttctgccttg tctcctttga catggacggt caccttagag tcaggctgac
    11101 ggtaacggaa tttagccgta cattctagcg taaactcttc tggcatttca cgagtaaagt
    11161 ggacttggct agcctctagg ctggttgaca tgagcgaatc atggtagaaa ccttggccta
    11221 catagagaat attcttgctt agatcttttc cgacaacgaa ccaaggggca ttgtcaccac
    11281 cgtgttgccc accgataccg agtccgccac gctgaccgat tgtatagtac ataagaccag
    11341 catgctcgcc catatcacga ccatccacag tcatcatacg accaggctga gctggcaggt
    11401 agttgctgag aaagttttta aagttctttt ctccgataaa gcaaatccct gtcgagtctt
    11461 tcttcttagc agtcgaaaga cctgcttctt ctgctagttt tcgaacttca ggctttttca
    11521 aatgtcctag tgggaacatg gttttttgaa gttgttcttg cgaaagttgg ctgaggaaat
    11581 aggtctggtc cttgccattg tccacgccac gaagcatgtg aacagtgcca tcctcatcac
    11641 gcgccactcg agcataatgc ccagtcgcta catagtctgc ccccaaggtt atggcatagt
    11701 ccaaaaaggc cttgaacttg atttccttgt tgcacataac gtccggattt ggcgtgcgcc
    11761 ctgcacggta ttccgctagg aaatactcaa aaacgcggtc ccagtactct ttttcaaaat
    11821 tgacagagta gtagggaatg ccaatctggt ctgccaccgc aaccacatcc ttgtaatctt
    11881 cggtcgccgt acagacgccg ttttcatctg tgtcatccca gttcttcatg aagataccga
    11941 tcacatcgta gccctgctcc ttgagcaaaa gagccgtcac cgacgaatca acaccaccac
    12001 tcatccccac gacaacacgt gttttagagt tatcactcat ggtaagtctc ccatctattc
    12061 gtttattcac gattgaaggt cgtgtgtgct tcacgattga aggtcgcttg agcagtattc
    12121 attataacac gcttggttag agaagacaag gaagagctta aaaattgttt tttaaaactt
    12181 atagtcaata tacagattat gtgaatgatg tgagtatacc ttcttcttat cttcattcaa
    12241 ataactataa taaataaaac aacccatttt ctctaatcta tttttcaagt tagagaaaag
    12301 gagtcgtttt tttaatgaca tttcctgaaa tcataagata ggtacctaaa gaatacgaac
    12361 ataaatctat tcaatcgaac cttatctttg tttcaaaatt aaaatgtcaa atgttcttaa
    12421 catgatttaa tttaatcggc ttaaacccat tcaccgtttt cattgacagt atatccgtct
    12481 acagttgtgt tgactgcaag ggcacctaag ccattgacat agtaccattt atctgatact
    12541 ttgaaccatt ggtttgcttt catagcacct gatgcttcaa gatagtacca ggtatctcca
    12601 tctttcaccc aacctgttgc cattgaaccg ttagcgttga ggtagtacca tgaaccgttg
    12661 actttagccc aacctgttgc catagcgccg ttagcgttta gatagtacca tgaaccattg
    12721 tattggagcc aacctgtcgc catagcgcca ttgctgttga ggtagtacca tgagccattg
    12781 tattctagcc aacctgtcgc catagcacca ttagcgttga ggtagtacca tgaaccattg
    12841 tattctagcc aacctgtcgc catagcacca ttagtattga ggtagtacca tgaatcattg
    12901 tattggagcc atcctgtcgc catagcgcca ttgctgttga ggtaatacca taagccgttg
    12961 actttagccc aacctgttgc catagcacca ttagcgttga ggtagtacca tgagccatta
    13021 ttttgtagcc agcctgttgc cattgaacca tccgtattgt agaagtacca cataccattt
    13081 tcttgtttcc agcctgtttt tggagtttct ggtttaggag ctggagctgg tttaggagcc
    13141 gg
//
LOCUS       DAVLXF010000052        13079 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLXF010000052 DAVLXF010000000
VERSION     DAVLXF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.052"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            31..1377
                     /gene="celB"
                     /locus_tag="VYO12_001412"
     CDS             31..1377
                     /gene="celB"
                     /locus_tag="VYO12_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEV1588004.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNSLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IIFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            1757..1933
                     /locus_tag="VYO12_001413"
     CDS             1757..1933
                     /locus_tag="VYO12_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588005.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALSLGMT"
     gene            2335..4548
                     /locus_tag="VYO12_001414"
     CDS             2335..4548
                     /locus_tag="VYO12_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1588006.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEDEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(4770..5246)
                     /locus_tag="VYO12_001415"
     CDS             complement(4770..5246)
                     /locus_tag="VYO12_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1588007.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTNPKQIEEAIQTLL"
     gene            5469..8672
                     /locus_tag="VYO12_001416"
     CDS             5469..8672
                     /locus_tag="VYO12_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1588008.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSTWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDIASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKSGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            9052..9437
                     /gene="tnpA"
                     /locus_tag="VYO12_001417"
                     /pseudo
     CDS             9052..9437
                     /gene="tnpA"
                     /locus_tag="VYO12_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9816..10445)
                     /locus_tag="VYO12_001418"
     CDS             complement(9816..10445)
                     /locus_tag="VYO12_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1588009.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(10455..11456)
                     /locus_tag="VYO12_001419"
     CDS             complement(10455..11456)
                     /locus_tag="VYO12_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1588010.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(11487..12128)
                     /locus_tag="VYO12_001420"
     CDS             complement(11487..12128)
                     /locus_tag="VYO12_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1588011.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(12147..12962)
                     /locus_tag="VYO12_001421"
     CDS             complement(12147..12962)
                     /locus_tag="VYO12_001421"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1588012.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         4088 a         2244 c         2781 g         3966 t
ORIGIN      
        1 attattatgt tttattaagg aggtctattt atggatcaac aaaacgggtt gtttggtttt
       61 cttgaaaacc atgttatggg accaatgggc aaacttgctc agtttaaagt agtgcgtgct
      121 atcacggctg caggtatggc tgctgtacca tttactattg taggatcaat gtttttggta
      181 ttcagtattt tgccacaagc tttctcattt tggccaattg tggcagatat tttctctgct
      241 tcatttgata aattcacatc actttacatg gttgcaaact atgcgactat gggttctcta
      301 tctctttatt tcgttctatc acttgcatat gaattgacaa aaatttatgc agaggaggaa
      361 gaactcaata tgaattctct taatggtgcc ttgctcgcct tgatggcttt tgtcatgaca
      421 gtaccgcaaa tcatttttga tggtggaatg atgaagactg tgacaagtct aaaagaaggt
      481 gcagtaattg cagatggatg ggcaatggga aatggagtcg cacgttttgg tacaacagga
      541 atttttaccg caatcattat ggcaattgtg actgttctta tttatcgtat gtgtgttaaa
      601 cataattggg ttattaaaat gcctgaagct gttccagaag gagtttctcg tggatttacc
      661 gctttggttc cgggatttgt tgttgcattt gttgttatct ttatcaacgg tcttcttgta
      721 gcaatgggaa cagatatttt taaagtcatt gcaattccat ttggttttgt atccaatctg
      781 actaattcgt ggattggttt aatgattatt tatctattga ctcaactact ttggattgta
      841 ggtatccacg gtgcgaacat tatttttgca tttgttagtc caattgctct tgctaacatg
      901 gctgaaaatg ctgctggcgg gcacttcgct gttgcaggtg aattttctaa tatgtttgta
      961 attgcaggtg gttctggtgc aactttagga ctatgtttat atattgcttt tgcctctaaa
     1021 tctgaacagc ttaaagcaat aggacgagca tctgtagttc cagccttatt taatattaat
     1081 gaaccattaa tttttggatt acctattatc tataatccag ccttggctat accatttatt
     1141 ttagcaccaa tggttactgc tactatttat tacgtagcga attctctaaa ctttattaag
     1201 ccaattatcg cacaggttcc atggccaact ccagtaggga ttggagcttt cttagggaca
     1261 gcagatcttc gagctgtatt agttgctcta gtatgtgcat ttgcagcatt cctagtctat
     1321 cttccattca tccgtgtata tgatcaaaaa ttggtgaaag aagagcaagg tatctaataa
     1381 gtaaaaaagt atactcgatg atagaatcca tatagtttaa ataatgagcg ttaacattag
     1441 cagtgaagag gtctttttaa gatattcttc tctgcttttt catatttttt cgctatgtag
     1501 agtatgaagc aaaatagaaa gtagttcgaa tagttgataa acagggaatt gctgttattt
     1561 aaaaaaaaca ataaagtaaa gtatgttatc gtagaatacg atacaatagt aaaaaagcaa
     1621 aaagaatgcg ttttatattt gggtttgagg cttataaaat aggataagcg tactgaaaac
     1681 ttagatatga caaacaaaat aaaattttta aaagctacag agaaacatgg tataatgtat
     1741 ctaaaggagg tagcatatgt ccttagatat agataaagaa aagatgacaa tcatgggaat
     1801 agcttttgaa aaccgatccg tttttaagag tgtttggtat gctttaagta caaatatgat
     1861 tgaaggatgg cgacctacgg ttagtgatgt tgaaaaatta cgagatgaag ctctttctct
     1921 ggggatgact taatgaagcg caactgttat gagtcttatg actatgttga tccggataat
     1981 ttatataccg atccgggttc ttcagttcta agaaataagc aggaaggaaa aaagcacgcg
     2041 agcttgaata tcgtatgatt gctagtaaga gtttgaaatt atttatcaat cctatcttag
     2101 ttaatagttt aaaagtagtt tgtttcacaa ctgcataagc ttagaaaagt tgaaaagtga
     2161 taactcttta ataaactctt ggtttgttaa aagatatttt gtccactaca tttttttcaa
     2221 ttgactatta tttgtcatag atataacaga tagaacattg cattcagaat aatgaaaaac
     2281 gcatcaaaaa tggtgcgttc tgtttttatg ctaccataaa aggaggaaaa caaattgaaa
     2341 atttttaagg gagagtttta tcgaatctct gtattaacag acaagctagt aaggttagaa
     2401 tactctcgaa ctggaaattt tgaggataga acgacacaac ttatctataa tagagatttt
     2461 ggccaagttt cgttagatta tatcgagaca tcaaatgtac tagatattat gacggactat
     2521 tttcatctgc actttaataa aggagaattt aacgccgaaa atttatttat agaattaaaa
     2581 ggaaattttg ccgtatatgg tagtcgctgg tattttggtg aatctattga aacgttaaaa
     2641 ggaacagctc ggactctgga taaggcagat ggagcaatct cgttagaaga tggaattatt
     2701 agccgaaatg gtatagcctt attggatgat tctcaaggat ttatttggga tgaacaatct
     2761 ggttatattg agagagaaaa tcaaattgac ctgtatttct ttgcctatgg gcatgattat
     2821 agaggagcaa tcagagactt ttaccatttg actggttcaa cacccttgtt gccaagatat
     2881 gctttaggca attggtggag tagatattgg ccttatacgt cggatgaata cttggattta
     2941 atagacagat ttgaaacaga gaaaattcca ttatctatcg gtgtgttaga tatggattgg
     3001 catataactg acattccagc tcgttttgga agtggctgga caggatatag ttggaataga
     3061 aacttaatac caaatccaga acagttattg caacaacttc atgatagaaa gttaaaactc
     3121 tccttaaatg tccatcctgc tgatgggata cgggcttatg aagaagctta tcctcaaatc
     3181 gcaaaacggt tggggttaaa tgtagaacta gaagaacctg ctatttttga tttttttaat
     3241 ccctctttta gggaagccta ctttaaagat gttcattatg agctagaaaa gcagggagta
     3301 gatttttggt ggattgactg gcaacaaggg acacaaggta tgctagatcc actttggctt
     3361 ttaaaccatt atcactatca ggatagttgt aaaaatgcag aaggtggttt gattttatca
     3421 agatatgcag gtcctggtag tcaccgctac cctgttggtt tttcagggga cactattatt
     3481 agttggaatt ccttaagatt tcaaccctat tttacagcga cagcatctaa tatcggttat
     3541 agttggtgga gtcatgatat cggtggacat atgttggggg attatgacga agagctacaa
     3601 actagatggc tacagtttgg tgtttttagt ccgattactc gattacatag ttctagaagt
     3661 ccttttaata gtaaagaacc ttggttcttt tcagaaacaa catctaagat tatgaagaaa
     3721 taccttcgtt tgagacatca gatgattccc tatctatata ccatgaatgt aaagacacat
     3781 gaggaaggtg ccccattaat cagtccaatc tattatttct atccagagaa tgatgagagc
     3841 tataatgttc caaaccaata cttttttgga acagaactga tggtggctcc cattgtagaa
     3901 aagatggatt tgacattcca atctgcaaaa gtagatgtat ggttccctga agatgaatgg
     3961 tatgacttct tttcagagaa aaaatacaca ggtggtgtga agttaagtgt ttatagggac
     4021 atctcgacta cgcctgtgtt tgcaaaaagt ggtgcaatca ttcccttggt tggttctgag
     4081 ataggtatgg gtgttgattt acctgaggtt gtagattggt atgtattccc agggaaacaa
     4141 cattcttttg aaatgcttga agatcaaaat ggtcaaagat ataaaacaag attatcaatc
     4201 gattgggaaa tgggaatggt agagttagca ttacaaggag attctagtat cgttccaagc
     4261 aatagaaaac atagaattca ttttaaagga acgaatgtgt ctataattga attgccaaat
     4321 aagaatgata cagctaaatt tgaatggaaa gataataaaa ggacatcttt aaatgacgaa
     4381 gtttttagac tactaaagac agcttctctt ccatatgaat taaaagatag attgttaaat
     4441 caattcatca atgcgaaaaa ctctcatgac ttaatgaata tcttgcatca tcaggataag
     4501 gaattgagag ggcgtttgtt ggaaatgata tttactagcc aaaactaatt gaaataatta
     4561 gaaagtcatt tgtttagtgt atatgaagat tctgtatggt attcgttatc actggtcaca
     4621 ttgaaaatgt gaaataagca cctacaacat agactttaca acacttcatg ttttatatca
     4681 tggggtgttt ttctatttaa gtcaaagccc atcaaaaaat ctcactattc atcaggttaa
     4741 aggaaaccta atcatagtga gattgtgaat tatagtagag tttgtatcgc ctcttcaatt
     4801 tgttttgggt ttgtttttga agaaaagcgt tcaaagactt gcccatcacg accaatgaga
     4861 aacttagcga aattccattc gattcgtttt cctagtgggc cagatttatg gtcttttaac
     4921 cagacataga gagggtctgc ttccttaccg ttgaccttaa tcttggcaaa acgtgggaag
     4981 gtggtttgaa aatgtaggct acagaaggcg ttgatttcct ctgcgctgcc gggtgcttgt
     5041 cccataaact gattgcaagg gaaatccaat atttcaaagc cctgatcttg atagcgttca
     5101 tagagttctt gaagtccctg gtactggggc gttaaaccac atccagtagc agtgttgaca
     5161 atcaagagaa ctttaccacg atagctatcc aagggagttg cttggttgtt ttggttcaaa
     5221 acggaaaaat cataaagtga agtcatgagg gcctcttttc tttttctaca ttgtaacagt
     5281 ttttactttt ttcgtcgagt cggatggaag atattgttga agaataagcc ttcaatatga
     5341 tagaaaccgt ttacaaataa ctttaaaaat ggtatgattt aattaaaccg gtttactttg
     5401 ttgctataaa tctctaattt gagatttagt tcctattgat tttacaatat gtttattgga
     5461 gtgtatacat gcaaacaaaa acaaagaagc tcattgtgag tttgtcttca cttgttttat
     5521 caggattttt attaaaccat tatatgacag ttggagcgga agaaacgact acgaatacca
     5581 ttcagcaaag ccagaaggaa gttcagtatc agcaaaggga tacaaaaaat ttagttgaaa
     5641 atggtgattt tggtcagacg gaggacggaa gcagtccgtg gacaggaagc aaagctcagg
     5701 ggtggtcaac ttgggtagac cagaagaata gttctgcaga tgcctcaact cgagtcattg
     5761 aggctaagga tggggctatc actatctcaa gccatgagaa attaagggca gcgcttcacc
     5821 gtatggttcc tattgaagct aagaaaaagt ataaactgcg tttcaagatt aaaacagatc
     5881 ataaagtcgg gattgccaaa gttcgtatca ttgaggaaag tggtaaggac aagcgattgt
     5941 ggaattctgc aacgacgtca ggaacaaagg actggcagac cattgaagca gactatagcc
     6001 cgactttaga tgttgataaa atcaagctgg agttattcta tgaaacagga actgggactg
     6061 tttcctttaa ggatattgag ctggtagagg tagcagacca gccttctgag gattctcaaa
     6121 cagataaaca gcttgaggaa aagattgatt taccaattgg aaaaaaacat gttttttctc
     6181 ttgcggacta tacttataag gtagaaaatc ctgacattgc ttcagtcaaa aatggaattt
     6241 tagaacctct taaggaaggg acaaccaatg tcattgtcag taaagatggc aaggaagtga
     6301 aaaagattcc tttgaagatt ctagcctctg ttaaggatgc atacacagac cgtttggatg
     6361 actggaatgg catcatcgct gggaatcaat actatgattc taaaaatgaa cagatggcca
     6421 aattaaacca ggaattggaa ggaaaggtag ctgatagcct atctagtatt tcaagtcagg
     6481 cggaccgcac ctatttgtgg gaaaaatttt caaattataa aatgtctgca aatctgactg
     6541 ccacttatcg gaaattggag gagatggcca agcaagtgac caatccttct tctcgttatt
     6601 atcaagatga aactgtcgtt cgaacagtca gggattccat ggaatggatg cataaacatg
     6661 tctacaatag tgaaaagagc attgttggga actggtggga ttatgaaatc ggtacacctc
     6721 gtgccatcaa caataccttg tctctgatga aagaatactt ctctgatgag gaaattaaaa
     6781 aatatacaga tgtgattgaa aaatttgtac cagatcccga acatttccga aagacgactg
     6841 ataacccatt caaggctcta ggtggaaact tagttgatat gggaagggta aaagtaatag
     6901 ctggtttact gcgtaaggat gatcaagaaa tttcttctac cattcgctcg attgagcaag
     6961 tgttcaagtt ggtagaccaa ggtgaaggtt tttatcaaga tggatcctat atcgaccaca
     7021 ccaatgttgc ctatacgggt gcttatggga atgttttgat tgatggcctg tctcaactgt
     7081 tgccagtcat tcaaaagacc aagaatccaa tcgataaaga taaaatgcaa accatgtacc
     7141 actggattga taaatcgttt gctcctttgc tggtgaatgg agagctgatg gatatgagtc
     7201 gtggacgctc gatcagtcgt gcaaatagcg aggggcacgt ggccgcagta gaagtactaa
     7261 gagggattca ccgaatagcg gatatgtctg aaggagaaac caaacaacgt ttgcagagtc
     7321 ttgtgaagac cattgttcaa tcggatagtt attatgatgt ctttaagaat ttgaagactt
     7381 ataaggatat cagtttgatg caatccttgt taagtgatgc aggagtcgca agtgttccaa
     7441 gaacaagtta cctatctgcc tttaacaaga tggataaaac agccatgtac aatgcagaga
     7501 aagggtttgg atttggcttg tcactctttt ccagccgtac cttgaattac gaacacatga
     7561 acaaggaaaa taaaagtggt tggtatacga gtgatgggat gttctatctt tacaatggcg
     7621 atttgagtca ctatagcgat ggctactggc caacagttaa tccatataag atgcctggta
     7681 caacagagac ggatgctaag agagcggata gcgatacagg taaagtttta ccgtctgctt
     7741 tcgttggaac gagcaaacta gatgatgcca atgcgacagc aaccatggat ttcaccaact
     7801 ggaatcaaac attgactgct cataagagct ggtttatgct aaaggataag attgcctttt
     7861 taggaagcaa tatccaaaac acttcaacag atactgctgc aactacaatt gaccagagaa
     7921 aactggaatc aagtaatcca tataaagtct atgtcaatga taaagaagcc tcccttacag
     7981 aacaagaaaa ggattatcct gaaacccaaa gtgtcttttt agaatcgtcc gattcgaaaa
     8041 agaatattgg ttactttttc tttaagaaga gttcaatcag tatgagtaag gctttgcaaa
     8101 agggagcctg gaaggatatc aatgaaggac agtcagacaa ggaagttgaa aatgaatttc
     8161 ttactattag tcaggctcat aagcaaaatg gagattctta tggctatatg ctcattccta
     8221 acgtggatcg tgccaccttc aatcaaatga taaaagagtt agaaagtagc ctcatcgaaa
     8281 ataacgaaac ccttcagtct gtttatgatg ctaaacaagg agtttggggc attgtgaaat
     8341 atgatgattc tgtctctact atttccaacc aattccaagt tttgaaacgt ggagtctata
     8401 ccattcgaaa agaaggggat gaatataaga ttgcctacta taatcctgaa acccaggaat
     8461 cagctccaga tcaggaagtc tttaaaaagc tagagcaagc agctcagcca caagtacaga
     8521 attcaaaaga aaaggaaaaa tctgaagaag aaaagaacca ttcggatcaa aagaatctcc
     8581 ctcagacagg agaaggtcag tcaatcttgg caagtctagg gttcttgcta cttggggcgt
     8641 tttatttatt ccgtagagga aagaacaact aatttgttca tcatctagat gaattacatg
     8701 aaactgtgga aaatagaaaa aatccgaaga aggccaaata tcttcggatt tttcttttaa
     8761 aaagggataa gttgatactt atcctttttt acttcattat gtgactatag cgttttgaaa
     8821 ctagaatagg acgtcataac tgctaaaaga tttctataaa ttcatttgat tttcctaatc
     8881 aatttgttcg tatcctattt cactccacta tattagtccg tctcgctccg tgaggtgcga
     8941 gacaaaacaa ccacccgcta tgcgggtgcg cgtcgaaggc tataccaaaa aactccaaac
     9001 gcgatacaat aaaggtgttc aatccaattg taaagcgaaa ggagaaaaaa tatggcacaa
     9061 aagactcata gtttatcata cacaaagtgg atgtgttcta tcatattgtg ttcaccccta
     9121 agtatagacg aaaagtcatc tataatcaat atagaagtag tttaggcgaa atatttcatc
     9181 gcttgtgtgg ttataaaggt gttgaaatga tcgagggtca cttaatgcca gaccatgtac
     9241 acatgttagt cagtattcca ctaaggctaa gtgtttcgag tttcatgggg tatttaaaag
     9301 gcaaaagtgc actcatgatg tttgacaaac acgccaatct caagtacaag tttgggaatc
     9361 ggcatttctg ggcggaaggt tattatgtaa gtacagtagg gcttaataaa gccacactta
     9421 agaaatatag tcaagattaa agaaattatc gagtggatga tttcttcacg agtatgaaaa
     9481 tttgagaacg agtaaagtat gatatatagt aaaatgaaat aagaacagga caaaatcgat
     9541 caggacagtc aaatcgattt ttaacaatgt tttagaagta gaggtgtact attccagttt
     9601 caatctacta tagcactaga taaattgagt gtaaaagaat atgaggatcc ctttagggat
     9661 agtggtaagt aataccaaag tctcttaaag aggcaagtga cgagtcaaga gcaataaggc
     9721 ttgaacaacg tgaaaaccag cgtctttagg cgctggctgg taatttgggc ttatagctct
     9781 gggataaacc acccgtttga cgggtggtta tgattttatc tgagtgtaac atactgttgg
     9841 gcaatctcgc tgatgcggtc aaagttgcct tgggaagcga gtttattgag ttcgccacca
     9901 attccaacgg cgtctgcacc agcagcgaac cattgaggga tgttgtttag accgactcct
     9961 ccggttacca ttacggaaac ttgtgggatc ggtgccttga ctgcagagat atatgctgga
    10021 ctgagagcac tacctgggaa gagtttgatg atttcactac cggcttcaag tgcagtcgtg
    10081 atctctgtga gggtaataca gcctggaatg tacggtgtgc tgtagagatt gcacattttc
    10141 gcagtttcag catggaaaga tggagaaaca acgtaatttg ctccggctag aatggcatct
    10201 ctagcagtta cggcatcaag cacagtacct gcaccgatac aaacactctg atcgtcctga
    10261 tacaagtcta caagttcctt gatgatttgt cctgcatact gattggtata ggcgatttca
    10321 atagctttga taccgccctt gatacaagca atcgaggctt gcagtccttc ttcctttgta
    10381 tttcctcgaa tgacagcgac aattttctgt ttttttagtt caataatcgt atctgatttg
    10441 gtcatgtaat tctcctaacg aatgatatct tgtgcatttg ccagtaaatt ttcaatacta
    10501 gttgcggaag tggagagatg gtctcctgga agagtgcatt tgagagttgc gctcgcaact
    10561 gcaaagtcaa tggtagtttt tagggaggaa tgatggagta gttggtaaag ggcaccagat
    10621 ataaaggcat ccccgctacc aattcgttga tagactgcag tttttagttg gacagactct
    10681 tcaaatcgtt cttccagagc ataggcttga tagacatttt tgtcttgctc atcactagag
    10741 cggagggtat ggaaaatggc cttgaaacca taggcttctt ttaaaagtcg catgcgattt
    10801 tccacctctt ctaggctagc actgtctctt ggaaacatct ctagattttg gtcatcaatc
    10861 atgagaggat caataccgaa gcaatagtca gtaaaacgtg caaacttaga aaattcatac
    10921 ttggcttcta ggactgaaat catctttgtt ctcagattga gatccattga aacgacaatt
    10981 cctcggcgct tggcttcttc caagagtagg agaaggatcg cacggacctc ttgaccgata
    11041 gctacggtga ttccactaaa atgaaaatgg ctaatcccct gaaagagaga atccatatct
    11101 agcatgtttg gccgaatctg gctgatactc gtatgcttac gatcgtagaa aacttcactt
    11161 tgacgacaac caaagccgtt ctccaaatag tagaggccga ttcgatcgcc aagccgacag
    11221 attgaactgg tatcgatttg gtgctgtttc aagaatgtga gaaaacgatc tccaatctcg
    11281 ttggcaggga gtgcggtaaa gactttcgtt gagataccca gggcttgcaa attacaagcg
    11341 atgttaattt ctgatccgcc aaaataagtc gaactggcaa catgatcgcc gatactggtg
    11401 gcatctaatg gtgagattcg aattaatggt tcgccaaaca gtaatatttt cgtcatccat
    11461 tagttccttt ctggtttaga atagctttac ttggctaata cttctttcaa ataagcagcg
    11521 acttcatcat cttggcagtt ttcaaagaag tattcttgga aatatttgcc agagatggtg
    11581 tctttgagga agtcttggtc gattgtttta aggatggtca tcaaatcatt gtgggtgatt
    11641 tgtttcacct cgtttaagat acgagcgttg cgttgttcag ggattacacg ttctcttggg
    11701 tagccaccgc ccatttcttc agcaaataag cgttcaaaca tcaatttgag ggtcagttct
    11761 gccccccagc caaatccttt ggcataaggg atagacaagg cgttaccacc attgatttga
    11821 gaataaaggt aagcgtcagt tgggtccact gctagaccac agacaacacc agggaagctg
    11881 tttaaagcaa gcatagcccc tacacccgta ccacagccgg taacaacaaa gtcaactgcc
    11941 tttgtattta aaaggatggc agccattagt ccgttctgca cataagttaa ttgactttct
    12001 ccttcttctc cacgcatacc atagttaaat aattggtagc cttttttatc tgtcgcttct
    12061 tttagactat cgtaaataat gtgattcttg ctagcttgac tattttcatt gattaatgcg
    12121 attttcataa gatcttcttt ctatttttac tcaggttgtt ttccgatgta ggctaagata
    12181 ccgccatcta catataggat gtggccattg acaaaattgc tggcatcact agcgagaaag
    12241 acagcagggc ccatcaaatc ttcaggattt ccccaacgtg cagcaggtgt ttttgcaatg
    12301 atgaactggt caaatgggtg gcgagaacca tcttcttgca actcacgaag aggtgctgtt
    12361 tgaggagtgg caatataacc cggtccaatt ccgttacatt ggatattggc tccaccgtat
    12421 tcagacgcaa tgttgcgggt caacattttc aagccccctt tagcagcagc ataagcgcta
    12481 actgtttcac gtcccagttc gctcatcatc gaacaaatat tgataatctt tccatgccct
    12541 ttctttatca tagaaggaat aactgccttt gaaacgataa atggtgcgtt taagtcaata
    12601 tcgatgacct tacggaaatc agcggcgctc atttcgcaca ttggaactcg gcggataata
    12661 ccagcgttat taacgaggat gtcaatgaca ccaacctctt gttcgatttg cttgaccatg
    12721 gcttggatac cgtcctcgtc tgtcacgtca cagacatatc catgggcttc gatgccaact
    12781 tcacgataag cttcaatccc tttattgacc agatcttgat tgatatcgtt aaagacaata
    12841 gtagcgccgg cttcagcgta ggatttggca atagcaaatc caattccata agaagcgccg
    12901 gtgatgagag caatttttcc ttttaaagaa aactgctcaa ttgagaatga tgtatttgtc
    12961 atttgatact tcctttcaaa tttgttaaga cttctctata accaaattat attctagtaa
    13021 accggttttg tcaataaata aatattttta tttataaaaa gatttatttt ccttaaaaa
//
LOCUS       DAVLXF010000053        12914 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLXF010000053 DAVLXF010000000
VERSION     DAVLXF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.053"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            70..630
                     /locus_tag="VYO12_001422"
     CDS             70..630
                     /locus_tag="VYO12_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1588013.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            631..2016
                     /locus_tag="VYO12_001423"
     CDS             631..2016
                     /locus_tag="VYO12_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588014.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILLQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVAASPELFHMNRVTGELGNRSLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            2018..2668
                     /locus_tag="VYO12_001424"
     CDS             2018..2668
                     /locus_tag="VYO12_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1588015.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFLPSIALNLLVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            2679..3437
                     /gene="tenA"
                     /locus_tag="VYO12_001425"
     CDS             2679..3437
                     /gene="tenA"
                     /locus_tag="VYO12_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1588016.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEEWKHEKAPTTSSQINHFIYDDCP"
     gene            3376..3900
                     /gene="thiW"
                     /locus_tag="VYO12_001426"
     CDS             3376..3900
                     /gene="thiW"
                     /locus_tag="VYO12_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1588017.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            3900..4703
                     /locus_tag="VYO12_001427"
     CDS             3900..4703
                     /locus_tag="VYO12_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1588018.1"
                     /translation="MQEFTNPFPIGSSSLIHCITNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            4696..5328
                     /locus_tag="VYO12_001428"
     CDS             4696..5328
                     /locus_tag="VYO12_001428"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1588019.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKIETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(5537..6328)
                     /gene="thiD"
                     /locus_tag="VYO12_001429"
     CDS             complement(5537..6328)
                     /gene="thiD"
                     /locus_tag="VYO12_001429"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1588020.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(6412..6510)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6694..7089
                     /locus_tag="VYO12_001430"
     CDS             6694..7089
                     /locus_tag="VYO12_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1588021.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVDKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            7100..7471
                     /locus_tag="VYO12_001431"
     CDS             7100..7471
                     /locus_tag="VYO12_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1588022.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKRSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            7481..9724
                     /locus_tag="VYO12_001432"
     CDS             7481..9724
                     /locus_tag="VYO12_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1588023.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKEIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNILGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            9931..11706
                     /gene="spxB"
                     /locus_tag="VYO12_001433"
     CDS             9931..11706
                     /gene="spxB"
                     /locus_tag="VYO12_001433"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1588024.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            11817..12164
                     /locus_tag="VYO12_001434"
     CDS             11817..12164
                     /locus_tag="VYO12_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1588025.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(12281..12914)
                     /locus_tag="VYO12_001435"
                     /pseudo
     CDS             complement(12281..12914)
                     /locus_tag="VYO12_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3671 a         2502 c         2857 g         3884 t
ORIGIN      
        1 gtctgctata caaagtattt aggtcggtct tcttgagtgc cccacttttc tttaaaaaaa
       61 ggagttttta tgttgaaaaa atggcagtta aaagatgtta tcttgcttgc tttcttgtct
      121 atcttttttg gtggggtttt cgttggttca ggatatgtgt ataatattct cagtctactc
      181 ttaacacctc ttggtttgca ggcctttgcc aatgaaatcc tcttcggtct ctggtgtatg
      241 gctgcgccca ttgctgccat ctttgttcca agagtcggaa gtgcaacgat tggagaagtg
      301 ctagctgcgc ttgctgaagt cctttatggt agccaatttg gtctaggagc tcttttgtct
      361 ggcttggttc aaggtttggg aagtgaattt ggttttatcg taactaagaa tcgctatgaa
      421 agttggctct ctctaactgc taatagtatt gggattacgc ttgttagctt tgtctatgaa
      481 tacattaagt taggttacta cgccttttcc cttccgtttg tcctttcctt acttgtggta
      541 cgttttattt ctgtttattt cttctgtacc atcttggttc gtgccattgt caaactctat
      601 catcagtttg caactggagg aaaagcatag atgggtctgg aactacgagc gattcagtcc
      661 ccaatcttct ctgagccgtt tgattttact tttcatgcgc aagcctttac cttgttagtt
      721 gggagcagtg gttcaggaaa atccagcctt tttcaagtca ttgcccaagt cagttctctt
      781 ccctatagtg gtcaagtcct gatagatggg agcgaggtca gtcagctttc tatcatcgca
      841 cgtgtccaga aggttggcat tctcttgcaa aatcccaatc atcaatttac catggagaac
      901 ttgtttgagg agttgatttt caccttggag aatatcggct atcaccttca ggaaattgat
      961 tctaaaatag cagaggttgt ccagcaatgt cgttgcgagg caatcttaca ccgtcccatt
     1021 catcacctat caggtgggga aaagcaaaaa gcagcgctgg ctgtcctctt tgccatgaat
     1081 cctagggtct atctcttgga tgagcccttc gcttccattg accgcaagag tagaatcgag
     1141 atattggaga ttctaaaaga gttggctctt gatgggaaga cagtcatttt gtgcgaccat
     1201 gatttatctg actataaagc ctatatcgac catatggttg agctaagaga cggaaaacta
     1261 agggaagtgt ttcaaatccc ttcctatgag atgacacagg ttgcttcaaa ggaagttgct
     1321 gctagcccgg aattattcca tatgaaccgt gtgactggtg agcttggaaa tcgctccctc
     1381 ttttcgattg ctgatttcac attctatcaa gggatttcct gtatcctggg tgacaatggt
     1441 gtcgggaaat caaccctctt tcgctctatt cttcaatttc aaaagtataa ggggcgcatt
     1501 acttggaagg gttcggtcct gaaaaagaaa aagagtttgt atcgtgatct gactggtgtt
     1561 gttcaggaag ctgagaagca gtttatccga gtcagtctgc gagaggagct tcaattagat
     1621 ggacctgatt ctgaaagaaa tcagcggatt tttcaagctt tacgatattt tgatttggag
     1681 caggcagtcg ataagagtcc ctatcaatta agtggtggtc agcaaaaaat tcttcagctc
     1741 ttgaccatct tgaccagtaa ggcttccgtg atcttgctag atgaaccttt tgcaggtttg
     1801 gatgatagag cctgccatta tttttgcaag tggattgtgg aggagaggaa tcaaggaaga
     1861 agttttctgc tcattagtca tcgtttagac cctttgattt ctgtggttga ttattggatt
     1921 gagatgacta gtcagggtct tcggcatgtg aaagaagtga ccattaccaa accacttaca
     1981 tctcagagta gcaataccca aggggaggtg agatagtatg gtcaaagtag caacccagac
     2041 accgattatc agtctcttct tgctgatttt atccttggaa acatctttcc ttccttcgat
     2101 tgctctgaat cttttggtag tcgcattttg tattctcttt atgctctatt accgtcgatt
     2161 taaaatgtta gcttggatga tcatacttgc cattttacca tcttttgcca actactgggc
     2221 agttcagtta cacggagatg cttcacaggc agtcatgctt ggaacgaggg cctttgtgac
     2281 agtttgtatc ggccttgtct ttgtttcctc tatttcacta aaagagcttc tcttgtactt
     2341 ggctcaaaag gggctatcac gctcttggtc ctatgccttg attgtggtat tcaattcttt
     2401 tcctctcatt cagcaagaaa tcaagtccct caaagaagct tgcctattac gtggtcaaga
     2461 actacatttt tggtcgccct tgatttacag taaggttctg atgacagtct ttaggtggcg
     2521 ccatctttac ctgagagctc tatctgctca cggatatgac gaacatgcac agttgaagaa
     2581 tagctatcgg actttttata ttcctaaaaa aacaaaatta atctacctgc ttttcttttt
     2641 attgcttcaa accagtctat ttttataaag gagttattat ggaatttaca gatattgcga
     2701 tggaattatc caagaaagct tggcaggctt cctttcatca tccctttatt ttacaattac
     2761 aagaggggaa tttagaacct gccattttcc gctattacct gattcaggat gcctactatc
     2821 tgaaggcctt ctcagaaatc tatcatcttt tggctgataa gacttcaaac caagagatga
     2881 aaagactctt gaaacaaaat gctcagggtt tagtggaggg tgagttattt attcgccaac
     2941 aatttttcaa ggaaatggaa atcagcgacc aggaaatgga gcaacatcca atcgctccaa
     3001 cctgttatca ttacatttct catatttata ggcaatttgc agaaccgaac ttagccatcg
     3061 cttttgcaag cttgcttcct tgtccttggt tataccatga tataggcaaa tcacttaatc
     3121 ttaaaccatc accaaatcct ctctatcaac aatggattga aacttatatt acggatgagt
     3181 tagagcaaca gatcagagag gagggagcac tggtcaatca gctctatcga gaaagtgacg
     3241 agacagacaa gcaaaaaatg ctagatgcct tccacatcag tgttcatatg gaagccaagt
     3301 tttgggaaat ggcctaccaa caccagacat ggaagagcga tttacagtct ttagaaaaag
     3361 gagaagaatg gaagcatgag aaagcaccaa ctacaagttc acaaattaac cattttatct
     3421 atgatgattg cccttgatgt agtccttaca cctatctttc ggattgaggg aatggcaccg
     3481 atgtccagtg tagtcaatat tctagcggga atcatgatgg gacctgttta tgccttggct
     3541 atggctacag tgacagcctt tatccgtatg acgactcaag ggattccgcc tttagctctc
     3601 acaggagcga cttttggagc ccttctagca ggtctctttt ataagtacgg tcgaaaattt
     3661 tacttttctg ccttgggaga aattgtggga acaggtatta ttggttctat tgtttcctat
     3721 cctgttatgg tactctttac aggatcagct gctaagctta gctggtttat ctacacgcct
     3781 cgatttttcg gagcaacctt gattggtaca gcgatttcct ttattgcctt tcgattttta
     3841 atcaagcagg aattctttaa aaaagtgcag ggatatttct ttagtgaaag gatagactga
     3901 tgcaggaatt tacaaatccc tttcctatag gctctagttc cctcattcac tgcattacca
     3961 atgagatttc ttgtgagatg ctggcaaatg ggattttggc tctgggatgc aaacctgtca
     4021 tggcagatga tccccgtgag gttcttgatt ttactaagca aagtcaggct ctcttcatca
     4081 atttggggca tttgtcagct gagaaggaga aagcaatccg tatagcagct tcttatgcag
     4141 ctcaagtttg tctcccaatg gtagtagatg cggttggcgt aacgacttca tccattcgta
     4201 agagcttagt taaagacctt ttagactata gacctacggt ccttaaagga aacatgtcag
     4261 aaattcgaag tcttgttgga ttaaagcacc acggcgttgg ggtcgatgcg agtgctaaag
     4321 atcaagaaac ggaggatttg cttcaagtct tgaaagactg gtgtcagacc tatcctggta
     4381 tgtcattctt agttactggc cccaaggacc tcatcgtttc ggagaatcaa gttgctgtac
     4441 tgggaaatgg ctgtgcagaa ttagactgga taacagggac aggagatttg gttggagcct
     4501 taacagccgt ttttctcagc caaggaaaga ctggttttga agcttcttgc ttagcagtct
     4561 cttatcttaa tatcgctgct gagaaaatag ttgttcaagg aatgggattg gaagaatttc
     4621 gttaccaagt actcaatcag ctttcgctcc taagaagaga tgaaaattgg ctagatacca
     4681 tcaaaggaga ggtttatgaa tagagaagca cttagactat atctggtaac caatcgctat
     4741 caagattccg tggaaagctt tcttgcaaaa attgagacgg cctgccgttc aggggttacc
     4801 atagtccaat tgcgagaaaa aaatctcaca accaatcaat attatcaact ggcaaaacaa
     4861 gtcaaggaaa taacagatgc ttatcaggta cccttgataa tcgatgatcg gttggatgtt
     4921 tgtcttgcgg ttgatgctgc tggtctgcat attggagatg acgaactccc agtttcggtt
     4981 gcccgacaag tcttgggtcc tgaaaaaatc ctcggtgtca ccgctaaaac ggttaaaaga
     5041 gctctggaag cggagaaatc aggtgcagat tacttgggga caggagccat ttttccgaca
     5101 accaccaagg aaaatgcacc catcaccctg atttcaacct tgaaaacaat ttgccaaacg
     5161 gttgccattc caatagttgc tattggcggc ttgacttcgg agaatattga tcagcttatg
     5221 ggcactggca tagctggtgt agctgttgta cgtgatttga tgcaagcaga agatattgag
     5281 gcaaaaacgc aagccttttt aacaaagttg catgacattc tttcctaatt aatactcaat
     5341 gaaaatcaaa gagtaaacta gaaagctagt cgcaggttgc tcaaagtact gccttgaggt
     5401 tttagataag actgacgaag tcagctcaaa acagtgtttt gaagttgcag atggaagctg
     5461 acgtagtttg aagagatttt cgaagagtat aaaaactctc taattccctt aaagtgggaa
     5521 ctagagagtt tttttcttaa tctttaaaag ttgtatggtt gactggacca gaaccatgac
     5581 cgagttgagg ggcatcttga atagcttttg tgataaaggc cttggcctta tcaactgcct
     5641 ggtaaagact cttgcccttg gctagttcag cagtaatcac tgcagcaaag gtacatccag
     5701 taccatgggt gtgacaggtt tgaattcgtg ggctttccca gacaaattgt tcattcttgg
     5761 taaagaggaa atctttagca ccacctttga gatgtccgcc tttgataacc acagactgag
     5821 gaccaaattc ttttaaaatc aggcgaccag cacgctgcat gtcttcgggg tcatggattg
     5881 aaaaaccaac aatctcttct gcttcaggaa gatttggcgt aataatagtt gctagaggta
     5941 gtaagtttgt tttgagatag tctctagcat ttgagtcaat caaggcatct ccacttgtag
     6001 caaccataac aggatcaagg acatagggac aatccagttt tttaagatag ggttggatga
     6061 tttccatgat ttcagtagta gccaacattc cagtttttac agcctgaggt ggaatatcag
     6121 aaaagacact ctccaattgg gctttcaaca tttgaggaga aacgtgctcg attagctgaa
     6181 cacctctggt attttgagcg acaagactgg ttacaacagc cattccatag acatctctcg
     6241 cttggaatga ctttaaatct gccataatgc cagcaccacc actagggtca gtccctgcaa
     6301 tggtcaaagc aacgggtaaa taagtcatct aaaacctccc aaccaactga agtttgtatt
     6361 cttaagaaca agctagtctg ttttagaaag gcaatagaag actgctagtc cccattatca
     6421 tttccacttc catcagctag cattacctag attgagtcag agggatctag cagtcgttaa
     6481 tctcagcttt acgcacccct agtggttgtt ttcttttttc tttagtatag catattttta
     6541 ttgtttatat agtaatattt gactgcaaat cgtttttcaa atgattaaaa ttctattttt
     6601 cgaagaaaat attatttcta taattgacaa atgtagattt taagagtata ctgataagtg
     6661 taattgacaa atgtagattt tggaggtatg gtaatgcaga tttcagatgc agaatggcag
     6721 gtcatgaaga ttatttggat gcagggggag cagaccagta cagatttgat tagggttttg
     6781 gcagagcggt ttgactggtc caagtccacg gttcagacac ttttggctcg tctggttgat
     6841 aaagagtgtt tgactcggaa aaaagaaggc aagtcctttg tttattcagc ccttttaact
     6901 ctagaccaaa gtcgggattt acttgtccaa gatatcaaag acaaggtttg ttcccgtagg
     6961 attaggaatt tgttggctga cttgattgta gaatgtgaat ttactcagac tgacttggaa
     7021 gacttggaag ctgtgatttc agagaagaaa tcaagtgctg taacagaagt aagatgtaat
     7081 tgtatgtaaa ggagacgtca tgttaaatag tattgtaacc attatttgta ttgctcttat
     7141 cgcgtttatc ttgttttggt ttttcaaaaa gcctgaaaaa tctggacaaa aagcccagca
     7201 aaaaaacgga taccaagaga ttcgagtgga agtcatggga ggctatactc ctgagttgat
     7261 tgtcctcaag agatcagtgc cagcccgcat tgtctttgac cgcaaggatc cttcaccatg
     7321 tctggatcaa attgtttttc cagattttgg tgtacatgcg aacctgccaa tgggggaaga
     7381 gtatgtagtg gaaatcacgc ctgagcaggc tggagagttt ggctttgctt gtggtatgaa
     7441 catgatgcac ggcaagatga ttgtagagta ggtggagact atgacagaaa ttgtgaaagc
     7501 aagcttagaa aatggcattc aaaaaatccg tatccgagct gaaaaaggct atcatccagc
     7561 ccatatccag cttcaaaagg aaattccagc tgagattacc tttcatcgtg ctactccttc
     7621 aaactgttat aaggaaattc tgtttgaaga agaaggtatc ttggaaccaa tcggcgtaga
     7681 tgaggagaaa gtcattcgtt ttacacctca agaattaggg cgacatgaat tttcttgtgg
     7741 catgaagatg caaaagggaa gctatatagt cgttgagaag actcgaaaat ctctatctct
     7801 cctgcaacgt ttttggatta ctagtatctt tactgtgcct cttgtgattc tcatgattgg
     7861 gatgttgaca ggtagcatta gtcatcaagt catgcattgg ggaacctttc tagcaacaac
     7921 gcctattatg ttagttgcag gtaagccata tatccagagt gcttgggcca gttttaaaaa
     7981 gcacaatgcc aacatggata ccttggttgc gctgggaact ctagtggctt atttctatag
     8041 cctagttgct ctctttgctg gtctccctgt ttacttcgaa agtgctggat ttatcctctt
     8101 tttcgttctt ttgggagcag tttttgagga aaaaatgaga aaaaatacgt cccaagctgt
     8161 ggagaaatta ctggacttgc aagctaaaac cgcagaagtc ttgagtgatg atagttatgt
     8221 ccaagttcct ttggaacaag tcaaggtagg cgacctgatt cgagtgcgtc ccggtgaaaa
     8281 gattgctgtt gatggtgtcg tagtagaagg tgtctctagt attgacgaat ccatggtgac
     8341 aggtgagagt ctgcctgtgg acaagacagt tggagatact gtcattggct caaccatcaa
     8401 tcatagtgga acgcttgtct ttagagcaga aaaagttggc tcagagactg ttttggctca
     8461 gattgtggat tttgtgaaga aagctcagac aagtcgtgcg ccgattcagg acttgacgga
     8521 taagatttca gggatttttg tcccagtagt tgtcatttta ggaatcatga ccttttgggt
     8581 ttggttcgtc ttgctcaggg atagtgtggt tgtgcttgga gctagctttg tgtcctctct
     8641 tctctacgga gtggcggttt tgattatcgc ctgtccttgt gccttgggac ttgcaacacc
     8701 gacagccctt atggtgggga caggacgtag tgccaagatg ggggttctcc tcaaaaatgg
     8761 aactgtctta caggaaatcc agaaagttca aacccttgtc tttgataaga ccgggacttt
     8821 gacggaaggg aaacctgtgg taacagatgt catcggcgac gaagtagaag tgtttgggtt
     8881 ggcagcctcc ttggaagatg cttctcaaca cccactggct gaggctatcg ttaagcgagc
     8941 gagtgaagct ggacttgagt ttcaaactgt tgaaaatttt cagtccttgc acgggaaagg
     9001 tgtttcaggg cgaatcaatg gaaaacaagt tttacttgga aatgctaaaa tgctggatgg
     9061 catggatatt tctaatactt atcaagataa actagaagaa ctagaaaaag aagctaagac
     9121 agttgtgttc ttagctgttg acaatgaaat caaaggcttg cttgctttgc aagatattcc
     9181 taaggaaaat gctaagctag ccatcagtca gctaaaaaaa cgtggtctcc gaacagtcat
     9241 gctgacagga gacaatgctg gtgtggcgcg tgctattgca gatcaaatcg gaattgaaga
     9301 ggtcattgca ggcgtcttgc cagaagaaaa agcccatgaa atccataaac tgcaacagtc
     9361 agggaaagta gcctttgttg gggacggtat caatgacgct cctgccctta gtgtagcaga
     9421 tgtgggaatt gctatgggtg ctggaacaga tatcgccatc gagtcagcag atttggtgtt
     9481 gacaaccaat aatattttag gagtggttcg tgcctttgat atgagtaaga aaacctttca
     9541 tcgaattcta ctcaatcttt tctgggcttt tatctacaat gttgtcggaa ttccgattgc
     9601 agcaggagtc ttttcaggtg ttggactggc tctcaaccca gaattggcag gtctagccat
     9661 ggcctttagt tctgtatctg ttctgaccag ttcccttctg ttaaatttta gtaaaataga
     9721 ctaaaagtaa tgactagatt tctttgttat aaaacagaaa tgacaacact ttcaaaactg
     9781 tattagaata gagaatagag agttttgagc agatttttag aaaagtcagc ataaatatga
     9841 tacagtggaa tagtaaaaat ttggagaacg tttccaattc tatgtaatcg tattctccaa
     9901 gtctaaaaaa attgaaggag agttatcatt atgactcaag ggaaaattac tgcatctgca
     9961 gcaatgctta acgtattgaa aacatggggc gtagatacaa tctacggtat cccatcagga
    10021 acactcagct cattgatgga tgctttggct gaagacaaag atatccgttt cttacaagtt
    10081 cgccacgaag agacaggtgc tcttgcagcg gttatgcaag ctaaattcgg cggctcaatc
    10141 ggggttgcag ttggttcagg tggtccaggt gcgactcact tgattaacgg tgtttacgat
    10201 gcagctatgg ataacactcc attcctagcg atccttggat cacgtccagt taacgaattg
    10261 aacatggatg ctttccaaga gcttaatcaa aacccaatgt acaacggtat cgctgtttac
    10321 aacaaacgtg tagcttacgc tgagcaattg ccaaaagtaa ttgacgaagc ctgccgtgct
    10381 gcagtttcta aaaaaggtcc agctgttgtt gaaattccag taaacttcgg tttccaagaa
    10441 atcgatgaaa actcatacta cggttcaggt tcatacgaac gctcgttcat cgctcctgct
    10501 ttgaacgaag ttgaaatcga caaagctgtt gaaatcttga acaatgctga acgcccagtt
    10561 atctatgctg gatttggtgg tgttaaagct ggtgaagtga ttactgaatt gtcacgtaaa
    10621 atcaaagcac caatcatcac aactggtaaa aactttgaag ctttcgaatg gaactatgaa
    10681 ggtttgacag gttctgctta ccgtgttggt tggaaaccag ccaacgaagt ggtctttgaa
    10741 gcagacacag ttcttttcct tggttcaaac ttcccattcg ctgaagttta cgaagcattc
    10801 aagaacactg aaaaatttat ccaagtcgat atcgaccctt acaaacttgg taaacgtcat
    10861 gctcttgacg cttcaatcct tggtgatgct ggtcaagcag ctaaagctat ccttgacaaa
    10921 gtaaacccag ttgaatcaac tccatggtgg cgtgcaaacg ttaagaacaa ccaaaactgg
    10981 cgtgattaca tgaacaaact cgaaggtaaa actgagggtg aattgcaatt gtatcaagtt
    11041 tacaatgcaa tcaacaaaca tgctgatcaa gacgctatct actcaatcga cgtaggtaac
    11101 actactcaaa catctactcg tcaccttcac atgacaccta agaacatgtg gcgtacatct
    11161 ccactctttg cgacaatggg tattgccctt cctggtggta tcgctgctaa gaaagacaat
    11221 ccagatcgcc aagtatggaa catcatgggt gacggagcat tcaacatgtg ctacccagac
    11281 gttatcacaa acgttcaata cgaccttcca gttatcaacc ttgtcttctc aaatgctgag
    11341 tacggcttca tcaagaacaa atacgaagat acaaacaaac acttgtttgg tgtagacttc
    11401 acaaacgctg actacgctaa aattgcggaa gctcaaggag ctgttggatt cacagttgac
    11461 cgtatcgaag acatcgatgc agttgttgca gaagctgtta aattgaacaa agaaggtaaa
    11521 actgttgtca tcgatgctcg catcactcaa caccgtccac ttccagtaga agtacttgaa
    11581 ttggatccaa aacttcactc agaagaagct atcaaagcct tcaaggaaaa atacgaagca
    11641 gaagaactcg taccattccg tctcttcttg gaagaagaag gattgcaatc acgcgcaatt
    11701 aaataattcc tctcgtcgaa aatcaaatat gaaacttgta gactttattc tttgattttc
    11761 atagagcaga actttaaaag atcggagcaa tccggtcttt ttatggagga gagtaaatga
    11821 atttaaatca attagatatt atcgtttcaa atgttcccca agtctgtgct gacttggagc
    11881 atatcttgga taaaaaggca gattatgctg atgatggttt tgctcagttt acgattggta
    11941 gtcactgcct tatgttgtca caaaatcatt tggttccttt ggaaaacttt cagtcaggaa
    12001 tcattattca tatcgaggtt gaggatgtag accagaacta caaacggttg aacgagcttg
    12061 gtataaaggt tttacacgga ccaactgtaa ccgattgggg aacagagtcc ttattagttc
    12121 aaggacctgc tggtctagtg cttgattttt atcgtatgaa ataggtcgcg tagtcatttg
    12181 acgttaacct tagctatttt taaaatagaa attgagaata tgaactattg aaagatcgga
    12241 gcaatccggt ctttttatat aaagaaattg catataatac tcatctgctt tcaaaaagca
    12301 ttctagtcca tctccgatta acgatggact ttatcacctc cttctccagt ccttgtatga
    12361 catcttgaag ttgattcatg acatcttcca aaattcgaaa ggctttattc ttaaatccac
    12421 gtttacgaat ctctttccac acttgttcaa tggggttcat ctctggtgtg tatggaggaa
    12481 taaatgcaaa accaatatta gtcggaatct ttaagatact tgatttatgc catatagcat
    12541 tgtccataac gagtaaaaaa taatcatctg gataagcttg tgaaagctct tctaaaaaga
    12601 cgttcatcca ctcagtatta catccaccag ctcttaagaa aaatgattcg cctgtatagg
    12661 catcaacagc tccataacaa tagcgaaatt ctcgtatata gtgactatgg acatgtggac
    12721 ctactcctat tggagaccaa caagatccca gtttactgat tctaccgaaa ccagcctcat
    12781 cttggtacat caagcgaacc ttatgaaaag gtctactggt tttaaagcgc tttcttgtct
    12841 tcttgaattg agattttatt tttagacgcg acaatggttt gagcgtctgc tttcttagga
    12901 tgttctggac gtgg
//
LOCUS       DAVLXF010000054        12769 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLXF010000054 DAVLXF010000000
VERSION     DAVLXF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.054"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            72..506
                     /locus_tag="VYO12_001436"
     CDS             72..506
                     /locus_tag="VYO12_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1588026.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAARGGVVNGKELFSTDAEEEEEDFESGI"
     gene            518..1708
                     /locus_tag="VYO12_001437"
     CDS             518..1708
                     /locus_tag="VYO12_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV1588027.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1719..2210
                     /locus_tag="VYO12_001438"
     CDS             1719..2210
                     /locus_tag="VYO12_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1588028.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2225..3004
                     /locus_tag="VYO12_001439"
     CDS             2225..3004
                     /locus_tag="VYO12_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1588029.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            2991..3809
                     /locus_tag="VYO12_001440"
     CDS             2991..3809
                     /locus_tag="VYO12_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1588030.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEVFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3809..4102
                     /locus_tag="VYO12_001441"
     CDS             3809..4102
                     /locus_tag="VYO12_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1588031.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4124..6025
                     /locus_tag="VYO12_001442"
     CDS             4124..6025
                     /locus_tag="VYO12_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1588032.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6085..7086
                     /locus_tag="VYO12_001443"
     CDS             6085..7086
                     /locus_tag="VYO12_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1588033.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKKLNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7196..7531)
                     /locus_tag="VYO12_001444"
     CDS             complement(7196..7531)
                     /locus_tag="VYO12_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588034.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7542..7784)
                     /locus_tag="VYO12_001445"
     CDS             complement(7542..7784)
                     /locus_tag="VYO12_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588035.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7800..7994)
                     /locus_tag="VYO12_001446"
     CDS             complement(7800..7994)
                     /locus_tag="VYO12_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588036.1"
                     /translation="MNRVKEFRKELGISQLELAKNIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8160..9110
                     /gene="rsmH"
                     /locus_tag="VYO12_001447"
     CDS             8160..9110
                     /gene="rsmH"
                     /locus_tag="VYO12_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1588037.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DRKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            9122..9439
                     /gene="ftsL"
                     /locus_tag="VYO12_001448"
     CDS             9122..9439
                     /gene="ftsL"
                     /locus_tag="VYO12_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1588038.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9443..11695
                     /gene="pbpX"
                     /locus_tag="VYO12_001449"
     CDS             9443..11695
                     /gene="pbpX"
                     /locus_tag="VYO12_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV1588039.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            11697..12677
                     /locus_tag="VYO12_001450"
     CDS             11697..12677
                     /locus_tag="VYO12_001450"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1588040.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLFAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
BASE COUNT         3849 a         2248 c         2907 g         3765 t
ORIGIN      
        1 aagataaaaa taaaggatta caatggggaa tataaagtaa accggtaaac ctaaacaaga
       61 aaggagaaaa gatgaaaatt gtacttgtag ggcatggaca ttttgctaca gggatttata
      121 gttctttaca attgattgca ggcaatcaag aaaatgtgga ggcaattgac tttgtggaag
      181 gaatgtcagc agatgaactc aagcaaaaaa tcttacttgc aatttcaaat gaagaagaag
      241 ttttaatcct aagtgatctc ttgggaggat cgccattcaa ggtttcttct accataatgg
      301 gagaaaatcc agccaaaaca atgaatgttc tctcgggttt gaacttagcc atgttaatgg
      361 aagcagtctt tgctagaatg gctcatagct ttgatgaggt tgttaataaa tcagtagtgg
      421 cggcccgggg cggagtcgta aatggtaaag aattgttttc aacggatgca gaggaagagg
      481 aagaagattt cgaatcgggt atttaaaggg taaaagaatg ataaaaaagg ttacgattga
      541 aaaaataaaa tcgcctgagc gcttcttaga agtaccactt ctgacgaaag aagaagtcgg
      601 ccaggcaatc gataaggtta ttcggcagtt agaactcaac cttgactatt tcaaggaaga
      661 tttcccgacg ccagctacct ttgataatgt ctatccaatc atggataaca cggaatggac
      721 caatggtttc tggacaggag aactgtggtt ggcttatgaa tacagtcaac aggatgcatt
      781 taaaaacatc gctcataaaa atgttctttc tttcctggat cgtgtcaata agagagtaga
      841 attggatcac catgatctcg gcttcttgta cacaccgtct tgtatggctg aatataagat
      901 aaatggagat ggagaggcta gagaagcaac cttgaaagct gcagataagt tgattgaacg
      961 ctatcaagaa aaaggtggtt ttattcaagc ttggggagac ttgggcaaga aagagcatta
     1021 ccgtttgatt atcgactgct tgctcaatat ccaactctta ttctttgctt atcaagaaac
     1081 aggcgatcaa aaatactacg atattgcaga aagtcatttc tatgcttcag ctaataatgt
     1141 aatccgtgat gacgcttcgt ccttccacac cttctatttt gatcctgaga caggtcaacc
     1201 ctttaaaggt gtaacgagac aagggtatag tgatgattca tgctgggcac gtggtcaatc
     1261 atggggagtc tatggtattc ctttgactta tcgtcactta aaagacgagt cctgctttga
     1321 cttgtttaag ggtgtgacca attatttctt gaatcgtctg ccaaaagatc atgtgtccta
     1381 ttgggatttg atttttaatg atggtagtgc tcaatcacga gattcttcag caacagctat
     1441 cgccgtctgt gggattcatg aaatgctaaa acatctccca gaggtggatg ctgacaaaga
     1501 tatttataaa catgctatgc atgccatgct tcgttccttg atcgaacatt atgcaaatga
     1561 tcaatttacc cctggtggga caagtctcct ccacggtgtg tactcatggc attcaggtaa
     1621 aggagtggat gaaggcaata tctggggtga ctactattac ctagaagccc ttatccgttt
     1681 ctacaaagac tggaacctat attggtagga ggagaaatat gacaatgcca aatattatta
     1741 tgacccgtat cgatgaacgg ttgattcatg ggcaaggaca actttgggta aaatacctag
     1801 gttgtaatac ggtcattgtt gccaatgacg aagtaagcac agacaagatg cagcaaactc
     1861 tgatgaaaac agttgtgcca gactcagttg ccatgcgttt cttccctttg caaaaggtga
     1921 ttgatatcat tcacaaggct aatcctgctc aaacgatctt tatcgttgta aaggatgtga
     1981 aggacgcttt aaccttggta gaaggtggtg tccctatcaa agaaatcaat attgggaaca
     2041 ttcacaatgc tactggtaaa gagcaagtga cacgctccat cttcctgggt gaagaggaca
     2101 aggcggccct caaggaattg agccaaactc atcaagtaac atttaatacg aaaacaactc
     2161 caacaggaaa tgatggagct gttcaagtca acattatgga ctatatttaa aagaggagat
     2221 cgttatgtcg attaatgtat ttcaagcgat tttaattgga ttatggacag ctttctgttt
     2281 tagtggaatg ctgttaggaa tttacaccaa tagatgtatt gttctgtcat ttggtgtcgg
     2341 aattattcta ggtgatttgc ctactgctct tgcaatggga gctattggtg aattggctta
     2401 tatgggattc ggtgttggtg ctggaggtac tgttccacca aacccaatcg gacctggtat
     2461 ctttggtacc ttgatggcta tcactagtgc tggtaaagtc agtccagaag cggctcttgc
     2521 cctctctact ccgattgctg tggcgattca attcttacaa actttcgcct acactgtacg
     2581 tgctggtgcg cctgaaacgg cgatgaagca cttgaaaaac cataatttga agaaattcaa
     2641 gttcactcta aatgcaacaa tttggttgtt tgcctttatt ggatttacct tgggttgctt
     2701 gggtgccctt tcaatggata ccttgttgaa actcgtagac tacattccac cagtattgct
     2761 tacaggtttg acagttgctg gtaaaatgct cccagctatc ggttttgcga tgatcttgtc
     2821 agtgatggct aagaaagagt tgattccctt tgtcttgttg ggatatgttt gtgcagctta
     2881 tctaaacatc ccaacaattg gtattgcaat tgtaggtact atctttgctt tgattgaatt
     2941 ttataacaag ccaaaaacag cggatcatgt ggtagaggag gaagcacacg atgactggat
     3001 ctaacaaatt aacaaaacgt gattatctta aaacgtcttt gcgggcattc ttttgtcaaa
     3061 atggatttaa ctatagtaac tatcaagggt tgggatatgc caatgtgatg tatcctgctt
     3121 tgaaaaaaca ctatggagag gatcaggaag ttttctacca agccttggaa gaaaactgtg
     3181 aattctataa taccaaccca cacttcctgc cttttattac cagcttgcat cttgtaatgt
     3241 tggaaaatgg ccgaccaaca aaagaaacac gtagcatcaa gatggccttg atgggaccat
     3301 tggcaggtat tggggattct ctttctcaat tctgtttagc tccattgttc tcaaccatcg
     3361 cagcttcgtt tgctcaagaa ggcttggttg tcggtccaat cttgttcttc cttgcgatga
     3421 atacgatttt aacagcgatt aaattgtcaa ctggtctgta tggatacaaa ctaggaacaa
     3481 ctgtgattga taaactaagc gaacagatgg caacgatttc tcgtattgcc aatattatcg
     3541 gtgtaaccgt aattgctggt ttggcagcga catctgttaa gattatggtg ccgattacct
     3601 ttgctgcagg ggaagttaaa gcagacgcta aacaaagtat cgtaagtatt cagggaatgc
     3661 ttgataaggt tgctccagct cttctaccag ccctatttac acttttagtt tactacttga
     3721 tcaaagaaaa gaaatggaca acatataaac tcgttatttt aacagttatc atcggaatta
     3781 tcggaagctg gcttaagatt atagcttaat ggatacaaca ttgttttatg gaatagtgat
     3841 tgtcttggca gtgagtcccc ttttactgtc aagctttcat tctattcgtc agcaaaagtt
     3901 gcttcgcaaa cagatggagc aacgacaaga gtatttagct tctttaacat ctggtgatga
     3961 agtgttgtta ttgtctggaa ttcatggaaa aatcatttct atcaaagatg acttggtctc
     4021 cttgcagatt gcaaaaggtg tggtcatcta tgtagaaaag gaaagtgtaa tgggaaagac
     4081 aaaagaactg ctttttaagt agttcttttt ctggtaatag ataatgaaag aagaaagaag
     4141 acaatttttt gaaagaatcg atggaaacca atgtcgtgat tatatcttgt cccactgttc
     4201 aaaagactat gagaaagtca agagttccct tgaacgcttg atggacaatc gttttatgtt
     4261 tgatagtcct tgggatatgg agccttgttc aaaaatccat caaatccagc cgatggtatg
     4321 ggatcaagta tttgaagatg atccagaatg ggcttatatg ctcaatcgac aagaatatct
     4381 cttgcagttt atgatagggt atctggtaga aggagataag gactatattc aaaagtgcaa
     4441 gttctttcta tttgattgga ttgagcaggt gagagaattt tctcctcaat ccttgatgac
     4501 tagaaccttg gatacgggta ttcgttcctt tacttggttg aaactactct tgcttctctt
     4561 gaaatttgac ttgctagagg agaaagaact agagaaaatt ttggtcagtc tagaaaagca
     4621 gattgacttt atgaaaagct actatcgcgc caagtacacc cttagtaact gggggatttt
     4681 acaaacaatt ccgatgcttg ctatctatca tttcttttca gataagatgg acctagaaga
     4741 agcttaccat tttgcttcag aggagttgaa acagcaaatt gagacacaga ttttaggaga
     4801 tggaagccag tttgaacagt cgattctcta tcatgtagag gtttataaag ccttgctgga
     4861 tttgtgtctc ttgcttccag acttgcaaga tagttaccaa gagttgctgg aaaagatggc
     4921 gacctatatt caaatgatga caggcttaga tggacgaact ttggcttttg gtgatagcga
     4981 ttctacagaa acgacagaaa ttttgagcct gtctgctgtg gttttgaaca aggaagacct
     5041 tcttaacggt ctggatgtta aagttgattt gcttagcctc ttgttcctgg ggcgagaaaa
     5101 ggtcaagcga ctgcaggaat ttgaaaggag agcttggcag cctaagtcca tgatctttga
     5161 agactctgga catgtctgta ttaaggatga acatcgttat ctatttttca aaaatggtcc
     5221 gctaggaagt gcccatagcc atagtgacga gaatagtttt tgcttacagt atcaaggcca
     5281 acctattttc atagatgctg ggcgttattc ttatcgggag atatatgaac gttatctctt
     5341 aaagagtgct tggagtcatt cgacctgcat tgtagatggg aaagctccgg aaagaatcac
     5401 aggatcctgg gaatatgaat actatcctca ctccctgttt tgtcaccata aagaaaggga
     5461 gggagtgcat tatattgagg gggcttattg gtcagcagaa cctgatttgc cttatcttca
     5521 cagaagaaaa atcctcatgt tggtagagga tgtctggctc ttggtggatg acatcaggtg
     5581 tcaaggtcag catgaggcgt tgactcagtt tatccttgac aaggatgtga cctatcaaga
     5641 tgggaaaatc aatcagttga gactatggag tgaagttgat tttgatttgg aagataccat
     5701 catttctcct aaatacaatg agcttgaaag aagtagcaaa ctcaccaagc gccaattctt
     5761 tgagaatcag atgctggatt ataccatcat tgcgcatgag agttttgaaa tcatccgtca
     5821 ttctgtctac cagacagatg atcgtgaagt ggaaaatgct ctggcttttg aagtgaaaaa
     5881 tgacgaaaca gacaagctga ttctgttatt aagcgaggat attggtgtag gtgaaaaatt
     5941 gtgcctcgtt gacggaacaa aaatgcgtgg aaaatgtcta gtatatgata aaataaatga
     6001 gagaatgatt cgcttgcagt gctagaaata ggcattttga atagtgaaca tgttataata
     6061 agtattagta ggaggtgttt tagattggag aagaaactga ccataaaaga cattgcggaa
     6121 atggctcaga cctcgaaaac aaccgtgtca ttttacctaa acgggaaata tgaaaaaatg
     6181 tcccaagaga cacgtgaaaa gattgaaaaa gttattcatg aaacaaatta caaaccgagc
     6241 attgttgcgc gtagcttaaa ctccaaacga acaaaattaa tcggtgtttt gatcggtgat
     6301 attaccaaca gtttctcaaa ccaaattgtt aagggaattg aggatatcgc cagccagaat
     6361 ggctaccagg taatgatagg aaatagtaat tacagccaag agagtgagga ccggtatatt
     6421 gaaagcatgc ttctcttggg agtagacggc tttattattc agccgacctc taatttccga
     6481 aaatattctc gtatcatcga tgagaaaaag aagaaaatgg tcttttttga tagtcagctc
     6541 tatgaacacc ggactagctg ggttaaaacc aataactatg atgccgttta tgacatgacc
     6601 cagtcctgta tcgaaaaagg ttatgaacat tttctcttga ttacagcgga tacgagtcgt
     6661 ttgagtactc ggattgagcg ggcaagtggt tttgtggatg ctttaacaga tgctaatatg
     6721 cgtcacgcca gtctaaccat tgaagataag catacgaatt tggaacaaat taaggaattt
     6781 ttacaaaaag aaatcgatcc cgatgaaaaa actctggtat ttatccctaa ctgttgggcc
     6841 ctacctctag tctttaccgt tatcaaaaag ttgaattata acttgccaca agttgggttg
     6901 attggttttg acaatacgga gtggacttgc ttttcttctc caagtgtttc gacgctggtt
     6961 cagccctcct ttgaggaagg acaacaggct acaaagattt tgattgacca gattgaaggc
     7021 cgcaatcaag aagaaaggca acaagtcttg gattgtagtg tgaattggaa agagtcgact
     7081 ttctaaaatg aaggaaaatg acttgcaatc tctgttaaga aataaaataa tcccacctag
     7141 aacaagctag gtgggattat ttgcctatga aatgagaaat tatgggagca agctcctaaa
     7201 tcaactgttt ttgatctact tctttaacta cttgataaaa gttatagaag taggccaaac
     7261 ttgaaatgat ggttacgact aggaatattg aaaatttcca ttggacaggg ttggttaaaa
     7321 gttgtggaaa ggatatgagg agaaagaaga gggctgcgtt gaggacaggt atccgttttg
     7381 attgtatttt ctcaagtcct ttattgagcg caggaagaaa gaggagtagg agtagtaaaa
     7441 ctgtatgaga aacagctcct gaaataaggg caaagaaaag gaaaatactg ataaaaacat
     7501 gaatgatcag tagtctagct agtgatttca taaggtacct cctaatcctg gtctttttta
     7561 gctcttgcaa tacgaagtga gtcgacaata tgtatcatca ctccgaaaaa gaaagctccc
     7621 agtatagttt taaaaatatg ttttgtattt agaagagaac tgataaaatt tggattttca
     7681 cttgttaggg tatcaatgag tggaattata aaaaatatca ctgttccata aatcgaacct
     7741 gctttcagac caggataacg taactgtttc ttttcttttt tcatgagttt gctccttttt
     7801 taaaaatctt cctcccaaaa gagactgttg aggtcagttt ggaggctgcg ggcgagattg
     7861 agacagagtt ccaaggttgg attgtacttg tcgttttcaa tcatattgat agtctgtctc
     7921 gagacaccga tattcttggc gagctcgagc tgggaaatgc ccagttcctt gcgaaattct
     7981 ttcacacgat tcatctgttc ttctttctga tttatgtcgt atatatttga ctatattata
     8041 gtcttttaaa cataaagtgt caagtatttt tgacatattt tttgaagaaa tagtagtctc
     8101 cttgtcctat ttgtctgaca agtgcaagct ggtcggattt gtggtaaaat agataagata
     8161 tgacaaaaga atttcatcat gtaacggtct tactccacga aacgattgat atgcttgacg
     8221 taaaacctga cggtatctac gttgatgcga ctttgggtgg agcaggccat agcgagtatt
     8281 tattaagtaa attaagtgaa aaaggccatc tctatgcctt tgaccaggat cagaatgcca
     8341 ttgacaatgc gcaaaaacgc ttggcacctt acattgagaa gggaatggtg acctttatca
     8401 aggataactt ccgtcattta caggcacgtt tgcgcgaagc tggtgttcag gaaattgatg
     8461 gaatttgtta tgacttggga gtgtctagtc ctcaattaga ccagcgtgag cgtggttttt
     8521 cttataaaaa ggatgcgcca ctggacatgc ggatgaatca ggatgctagt ctgacagcgt
     8581 atgaagtggt gaacaattat gactatcatg acttggttcg tattttcttc aagtatggag
     8641 aggacaaatt ctctaaacag attgcgcgta agattgagca agcgcgtgaa gtgaagccga
     8701 ttgagacaac gactgagtta gcagagatta tcaagttggt caaacctgcc aaggaactca
     8761 agaagaaggg tcatcctgct aagcagattt tccaggctat tcgaattgaa gtcaatgatg
     8821 aactgggggc ggcagatgag tccatccagc aggctatgga tatgttggct ctggatggta
     8881 gaatttcagt gattaccttt cattccttag aagaccgctt gactaagcaa ttgttcaagg
     8941 aagcttcaac agttgaagtt ccaaaaggct tgcctttcat cccagatgat cgcaagccca
     9001 agatggaatt ggtgtcccgt aagccaatct tgccaagtgc ggaagagtta gaagccaata
     9061 accgctcgca ctcagccaag ttgcgcgtgg tcagaaaaat tcacaagtaa gagggaaaaa
     9121 gatggcagaa aaaatggaaa aaacaggtca aatactacag atgcaactta aacggttttc
     9181 gcgtgtggaa aaagcttttt acttttccat tgctgtaacc actcttattg tagccattag
     9241 tattattttt atgcagacca agctcttgca agtgcagaat gatttgacaa aaatcaatgc
     9301 gcagatagag gaaaagaaga ccgaattgga cgatgccaag caagaggtca atgaactatt
     9361 acgtgcagaa cgtttgaaag aaattgccaa ttcacacgat ttgcaattaa acaatgaaaa
     9421 tattagaata gcggagtaag atatgaagtg gacaaaaaga gtaatccgtt atgcgaccaa
     9481 aaatcggaaa tcgccggctg aaaacagacg cagagttgga aaaagtctga gtttattatc
     9541 tgtctttgtt tttgccattt ttttagtcaa ttttgcggtc attattggga caggcactcg
     9601 ctttggaaca gatttagcga aggaagctaa gaaggttcat caaaccaccc gtacagttcc
     9661 tgccaaacgt gggactattt atgaccgaaa tggagtcccg attgctgagg atgcaacctc
     9721 ctataatgtc tatgcggtca ttgatgagaa ctataagtca gcaacgggta agattcttta
     9781 cgtagaaaaa acacaattta acaaggttgc agaggtcttt cataagtatc tggacatgga
     9841 agaatcctat gtaagagagc aactctcgca acctaatctc aagcaagttt cctttggagc
     9901 aaagggaaat gggattacct atgccaatat gatgtctatc aaaaaagaat tggaagctgc
     9961 agaggtcaag gggattgatt ttacaaccag tcccaatcgt agttacccaa acggacaatt
    10021 tgcttctagt tttatcggcc tagctcagct ccatgaaaat gaagatggaa gcaagagctt
    10081 gctgggaacc tctggaatgg agagttcctt gaacagtatt cttgcaggga cagacggcat
    10141 tattacctat gaaaaggatc gtctgggtaa tattgtaccc ggaacagaac aagtttccca
    10201 acgaacgatg gacggtaagg atgtttatac aaccatttcc agccccctcc agtcctttat
    10261 ggaaacccag atggatgctt ttcaagagaa ggtaaaagga aagtacatga cagcgacttt
    10321 ggtcagtgct aaaacagggg aaattctggc aacaacgcaa cgaccgacct ttgatgcaga
    10381 tacaaaagaa ggcattacag aggactttgt ttggcgtgat atcctttacc aaagtaacta
    10441 tgagccaggt tccactatga aagtgatgat gttggctgct gctattgata ataatacctt
    10501 tccaggagga gaagtcttta atagtagtga gttaaaaatt gcagatgcca cgattcgaga
    10561 ttgggacgtt aatgaaggat tgactggtgg cagaatgatg actttttctc aaggttttgc
    10621 acactcaagt aacgttggga tgaccctcct tgagcaaaag atgggagatg ctacctggct
    10681 tgattatctt aatcgtttta aatttggtgt tccgacccgt ttcggtttga cggatgagta
    10741 tgctggtcag cttcctgcgg ataatattgt caacattgcg caaagctcat ttggacaagg
    10801 gatttcagtg acccagacgc aaatgattcg tgcctttaca gctattgcta atgacggtgt
    10861 catgctggag cctaaattta ttagtgccat ttatgatcca aatgatcaaa ctgctcggaa
    10921 atctcaaaaa gaaattgtgg gaaatcctgt ttctaaagat gcagctagtc taactcggac
    10981 taacatggtt ttggtaggga cggatccggt ttatggaacc atgtataacc acagcacagg
    11041 caagccaact gtaactgttc ctgggcaaaa tgtagccctc aagtctggta cggctcagat
    11101 tgctgacgag aaaaatggtg gttatctagt cgggttaacc gactatattt tctcggctgt
    11161 atcgatgagt ccggctgaaa atcctgattt tatcttgtat gtgacggtcc aacaacctga
    11221 acattattca ggtattcagt tgggagaatt tgccaatcct atcttggagc gggcttcagc
    11281 tatgaaagac tctctcaatc ttcaaacaac agctaaggct ttggagcaag taagtcaaca
    11341 aagtccttat cctatgccca gtgtcaagga tatttcacct ggtgatttag cagaagaatt
    11401 gcgtcgcaat cttgtacaac ccatcgttgt gggaacagga acgaagatta aaaacagttc
    11461 tgctgaagaa gggaagaatc ttgccccgaa ccagcaagtc cttatcttat ctgataaagc
    11521 agaggaggtt ccagatatgt atggttggac aaaggagact gctgagaccc ttgctaagtg
    11581 gctcaatata gaacttgaat ttcaaggctc gggctctact gtgcagaagc aagatgttcg
    11641 tgctaacaca gctatcaagg acattaaaaa aattacatta actttaggag actaatatgt
    11701 ttatttccat cagtgctgga attgtgacat ttttactaac tttagtagga attccggcct
    11761 ttatccaatt ttatagaaag gcgcaaatta caggccagca gatgcatgag gatgtcaaac
    11821 agcatcaggc aaaagctggg actcctacaa tgggaggttt ggttttcttg attacttctg
    11881 ttttggttgc tttctttttc gccctattta gtagccaatt cagcaataat gtgggaatga
    11941 ttttgttcat cttggtcttg tatggcttgg tcggattttt agatgacttt ctcaaggtct
    12001 ttcgtaaaat caatgagggg cttaatccta agcaaaaatt agctcttcag cttctaggtg
    12061 gagttatctt ctatcttttc tatgagcgcg gtggcgatat cctgtctgtc tttggttatc
    12121 cagttcattt gggatttttc tatattttct tcgctctttt ctggctagtc ggtttttcaa
    12181 acgcagtaaa cttgacagac ggtgttgacg gtttagctag tatttccgtt gtgattagtt
    12241 tgtttgccta tggagttatt gcctatgtgc aaggtcagat ggatattctt ctagtgattc
    12301 ttgccatgat tggtggtttg ctcggtttct tcatctttaa ccataagcct gccaaggtct
    12361 ttatgggtga tgtgggaagt ttggccctag gtgggatgct ggcagctatc tctatggctc
    12421 tccaccagga atggactctc ttgattatcg gaattgtgta tgtttttgaa acaacttctg
    12481 ttatgatgca agtcagttat ttcaaactga caggtggtaa acgtattttc cgtatgacgc
    12541 ctgtacatca ccattttgag cttgggggat tgtctggtaa aggaaatcct tggagcgagt
    12601 ggaaggttga cttcttcttt tggggagttg ggcttctagc aagtctcctg accctcgcaa
    12661 ttttgtattt gatgtaagaa tggcaccctg atgtttcagg gtgtttttgt gtttaaatac
    12721 acaatgaaaa tcaaagaaca aactagaaag ctaactttag gctgctcaa
//
LOCUS       DAVLXF010000055        12552 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLXF010000055 DAVLXF010000000
VERSION     DAVLXF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.055"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..1072
                     /gene="dnaN"
                     /locus_tag="VYO12_001451"
     CDS             <1..1072
                     /gene="dnaN"
                     /locus_tag="VYO12_001451"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1588041.1"
                     /translation="SSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNEDAG
                     LLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYP
                     RIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDS
                     HRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISF
                     YTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGV
                     VSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRP
                     FTLVPADTDEDFMQLITPVRTN"
     gene            1137..1331
                     /locus_tag="VYO12_001452"
     CDS             1137..1331
                     /locus_tag="VYO12_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1588042.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1415..2530
                     /gene="ychF"
                     /locus_tag="VYO12_001453"
     CDS             1415..2530
                     /gene="ychF"
                     /locus_tag="VYO12_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1588043.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLIESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYESEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2601..3170
                     /locus_tag="VYO12_001454"
     CDS             2601..3170
                     /locus_tag="VYO12_001454"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1588044.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     KADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3171..6680
                     /gene="mfd"
                     /locus_tag="VYO12_001455"
     CDS             3171..6680
                     /gene="mfd"
                     /locus_tag="VYO12_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1588045.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            6748..7014
                     /locus_tag="VYO12_001456"
     CDS             6748..7014
                     /locus_tag="VYO12_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1588046.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7007..7375
                     /locus_tag="VYO12_001457"
     CDS             7007..7375
                     /locus_tag="VYO12_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1588047.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7495..8763
                     /locus_tag="VYO12_001458"
     CDS             7495..8763
                     /locus_tag="VYO12_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1588048.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8760..10037
                     /gene="tilS"
                     /locus_tag="VYO12_001459"
     CDS             8760..10037
                     /gene="tilS"
                     /locus_tag="VYO12_001459"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1588049.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPKLEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKTQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10041..10583
                     /gene="hpt"
                     /locus_tag="VYO12_001460"
     CDS             10041..10583
                     /gene="hpt"
                     /locus_tag="VYO12_001460"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1588050.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEVDYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10599..>12552
                     /gene="ftsH"
                     /locus_tag="VYO12_001461"
     CDS             10599..>12552
                     /gene="ftsH"
                     /locus_tag="VYO12_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1588051.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNKARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4319 a         1891 c         2558 g         3784 t
ORIGIN      
        1 tagttctaaa aatgccattc ctattttatc aacagtaaaa attgacgtga ccaatgaagg
       61 tattacttta attggttcaa atggtcaaat ttcaattgaa aattttattt ctcaaaaaaa
      121 tgaagatgct ggtttgttaa ttacttcttt aggttcgatc cttcttgaag cttctttctt
      181 tatcaatgta gtatctagtt tacctgatgt aactcttgat tttaaagaaa ttgaacaaaa
      241 tcaaattgtt ttaaccagtg gcaaatcaga aattacccta aaaggaaaag atagcgaaca
      301 atatccacga atccaagaaa tttcagcaag cactccttta atacttgaaa caaaattact
      361 caagaaaatt attaatgaaa cagcctttgc tgcaagtaca caagagagtc gtccgatttt
      421 aacaggtgtc cacttcgtat tgagtcaaca caaagagtta aaaacagttg caacagactc
      481 tcatcgccta agccagaaaa aattgactct tgaaaaaaat agtgatgatt ttgatgtcgt
      541 aattcctagc cgttctctac gcgaattttc agcggtattt acagatgata tcgaaactgt
      601 agagattttc tttgccaata atcaaatcct ctttagaagc gaaaatatta gcttctatac
      661 tcgtctccta gaaggaaact atcctgatac agatcgcttg attccaacag actttaacac
      721 tactattact tttaatgtgg taaacttacg ccagtcaatg gagcgtgccc gtcttttatc
      781 aagtgcgact caaaatggta ctgtgaaact tgaaattaag gatggggttg ttagcgccca
      841 tgttcactct ccagaagttg gtaaagtaaa cgaagaaatc gatactgatc aggttactgg
      901 tgaagatttg accattagtt tcaacccaac ttacttgatt gattctctta aagctttaaa
      961 tagcgaaaag gtgactatta gctttatctc agctgttcgt ccatttactc ttgtgccagc
     1021 agatactgac gaagacttca tgcagctcat tacaccagtt cgtacaaatt aagtgaaaga
     1081 ggttgagcct ggctcgcctc ttttatgata taatcgaaaa agaaaaggag agtagtatgt
     1141 atcaagttgg aaattttgtt gagatgaaaa aatcacacgc ttgtacaatc aagtcgactg
     1201 gtaaaaaggc taatcgttgg gaaattacac gtgtaggagc agatatcaaa ataaaatgca
     1261 gtaattgtga gcatgttgtc atgatggggc gatatgattt tgagcgaaaa atgaataaaa
     1321 ttattgactg agaaccctta gttagagggt tagcacttta tccctttttg tgttataata
     1381 ttagggattg aaatgaaaac ggagaatgag aaatatggct ttgacagcag gtatcgttgg
     1441 tttgccaaac gttggtaaat caacactatt taatgcaatt acaaaagcag gagcagaggc
     1501 agcaaactac ccatttgcga ctattgatcc aaatgttgga atggtggaag ttccagatga
     1561 acgcctacaa aaactaactg aaatgataac tcctaaaaag acagttccca caacatttga
     1621 atttacggat attgcaggga ttgtaaaagg agcttcaaaa ggagaagggc tagggaataa
     1681 attcttggcc aatattcgtg aagtagatgc gattgttcac gtagttcgtg cttttgatga
     1741 tgaaaatgtg atgcgcgagc aaggacgtga agacgccttt gtggatccac ttgcagatat
     1801 tgacaccatt aacctagaat tgattcttgc agacttagaa tcagtcaata aacgatatgc
     1861 gcgtgtagaa aagatggcac gtacgcaaaa agataaagaa tcagtagcag aattcaatgt
     1921 tcttcaaaag attaaaccag tcctagaaga cgggaaatca gctcgtacca ttgaatttac
     1981 agatgaggaa caaaaggttg tcaaaggtct tttccttttg acgactaaac cagttcttta
     2041 tgtagctaat gtggacgagg atgtggtttc agaacctgac tctatcgact atgtcaaaca
     2101 aattcgtgaa tttgcagcga cagaaaatgc tgaagtagtc gttatttctg cgcgtgctga
     2161 ggaagaaatt tctgaattgg atgatgaaga taaaaaagag tttcttgaag ccattggttt
     2221 gatagaatca ggtgtagata agttgacgcg tgcagcttac cacttgcttg gattgggaac
     2281 ttacttcaca gctggtgaaa aagaagttcg cgcttggact ttcaaacgtg gtatgaaggc
     2341 tcctcaagca gctggtatta tccactcaga ctttgaaaaa ggctttattc gtgcagtaac
     2401 catgtcatat gaggatctag tgaaatacga atctgaaaag gccgtaaaag aagctggacg
     2461 cttgcgtgaa gaaggaaaag aatatatcgt tcaagatggc gatatcatgg aattccgctt
     2521 taatgtctaa aaattaataa atggtgtcaa ttaggttgga aaaaaattcc aacccttttg
     2581 gcttttgaaa ggaaaaataa atgaccaaat tacttgtagg cttgggaaat ccaggggata
     2641 aatattttga aacaaaacac aatgttggtt ttatgttgat tgatcaacta gcgaagaaac
     2701 agaatgtcac ttttacacac gataagatat ttaaagctga cctagcatcc tttttcctaa
     2761 atggagaaaa aatttatctg gttaaaccaa cgacctttat gaatgaaagt ggaaaagcag
     2821 ttcatgcttt attaacttac tatggtttgg atattgacga tttacttatc atttacgatg
     2881 atcttgacat ggaagttggg aaaattcgtt taagagcaaa aggctcagca ggtggtcata
     2941 atggtatcaa gtctattatt caacatatag gaactcaggt ctttaaccgt gttaagattg
     3001 gaattggaag acctaaaaat ggtatgtcag ttgttcatca tgttttgagt aagtttgaca
     3061 gggatgatta tatcggtatt ttacagtctg ttgacaaagt tgacgattct gtaaactact
     3121 atttacaaga gaaaaatttt gagaaaacaa tgcagaggta taacggataa atggtgacct
     3181 tattagattt attctcagaa aatgatcaga ttaaaaaatg gcatcaaaat ttaactgata
     3241 agaaaagaca actaatactt ggtttatcaa catctactaa ggctcttgca attgcaagca
     3301 gtttagaaaa agaagatagg attgtgttat tgacgtcaac ttatggagaa gcagaaggac
     3361 ttgttagtga tcttatatct atcttgggtg aggaactcgt ctatccattt ttggtagatg
     3421 atgctcctat ggtggagttt ttgatgtctt cacaggaaaa aattatttca cgggttgaag
     3481 ccttgcgttt tttgactgat tcatctaaga aagggatttt agtttgtaat atcgcagcaa
     3541 gtcgattgat tttaccgtct cccaatgcat tcaaagatag tattgtaaaa atctcagttg
     3601 gtgaagaata tgatcaacac gcgtttatcc atcagttaaa ggaaaatggc tatcgaaaag
     3661 ttactcaagt acaaactcag ggcgaattta gtcttcgagg agatatttta gatatttttg
     3721 aaatatccca gttagaacct tgtcgaattg agttttttgg tgatgaaatt gatggtatca
     3781 ggtcatttga agtagaaaca caattatcga aagaaaataa gacagaactc actatctttc
     3841 cagctagtga tatgcttttg agagaaaagg attatcaacg aggacagtca gctttagaaa
     3901 aacaaatttc aaaaacttta tcacctattt tgaaatcata cctagaagaa attctttcaa
     3961 gttttcacca aaaacaaagt catgcagact ctcggaagtt tttatctttg tgctatgata
     4021 agacatggac tgtctttgat tatattgaaa aagatactcc aatattcttt gatgattatc
     4081 aaaaattgat gaatcagtat gaagtctttg aaagagactt agcgcagtac tttacagaag
     4141 aattacagaa tagtaaagca ttttctgata tgcagtattt ttctgatatt gaacaaatct
     4201 ataaaaaaca aagtccagtg acctttttct ctaatcttca aaagggttta ggaaatctca
     4261 aatttgacaa aatttatcaa ttcaatcaat atcctatgca ggaatttttc aatcagtttt
     4321 cttttctaaa agaagaaatt gaacgatata aaaaaatgga ttacaccatt attctgcagt
     4381 ctagcaattc aatgggaagt aaaacattgg aggatatgtt agaggaatat cagattaaat
     4441 tggattctag agataagaca aatatctgta aagaatctgt aaacttaata gagggtaatc
     4501 tcagacatgg ttttcatttt gtagatgaaa agattttatt gataactgaa catgagattt
     4561 ttcaaaagaa attaaagcgt cgttttcgaa gacaacatgt ttcaaatgca gagagattaa
     4621 aagattacaa tgaacttgaa aaaggggact atgttgtcca tcatatccat gggattggtc
     4681 aatatctagg aattgaaacc attgaaatca agggaattca tcgcgattat gtcagtgtcc
     4741 aataccaaaa tggtgatcaa atttctatcc ccgtggaaca gattcatcta ctgtccaaat
     4801 atatttcaag tgatggtaaa gctccaaaac tcaataaatt aaatgacggt cattttaaaa
     4861 aggccaagca aaaggttaag aaccaggtag aggatatagc tgatgattta atcaaactct
     4921 actctgaacg tagtcagttg aagggttttg ctttctcagc tgatgatgat gatcaagatg
     4981 cctttgatga tgctttccct tatgttgaaa cggatgatca acttcgtagt attgaggaaa
     5041 tcaaaaggga tatgcaggct tctcagccaa tggatcgact tttagttggg gatgttggtt
     5101 ttggaaagac tgaagttgct atgcgtgcag cctttaaagc agtcaatgat cacaaacagg
     5161 ttgtcattct agttccgacg acggttttag cgcaacagca ctatacgaat tttaaggaac
     5221 gattccaaaa ttttgcagtt aatgttgatg tgttgagtcg ctttagaagt aaaaaagagc
     5281 agactgcaac acttgaaaaa ttgaaaaacg gtcaagtcga tattttgatt ggaacacatc
     5341 gtgttttgtc aaaagatgtt gtgtttgctg atttgggctt gatgattatt gatgaggaac
     5401 agcgatttgg tgtcaagcat aaggaaactt tgaaagaact gaagaaacaa gtggatgtcc
     5461 taaccttgac cgctacgcca atccctcgta ccctccatat gtctatgctg ggaatcagag
     5521 atttgtctgt tattgaaact ccgccgacta atcgctatcc tgttcagacc tatgttttgg
     5581 aaaagaatga tagtgtcatt cgtgatgctg tcttgcgtga aatggagcgt ggaggtcaag
     5641 tttactatct ttacaacaaa gttgacacaa ttgttcagaa ggtttcagaa ttacaggagt
     5701 tgattccgga ggcttcgatt ggatatgttc atggtcgaat gagtgaagtc cagttggaaa
     5761 atactctatt agactttatt gagggacaat acgatatctt ggtgacgact actattattg
     5821 agacaggggt ggacattcca aatgctaata ctttatttat tgaaaatgcg gaccatatgg
     5881 gcttgtcaac cttatatcag ttaagaggaa gagtcggtcg tagtaatcgt attgcttatg
     5941 cttatctcat gtatcgtcca gaaaaatcaa tcagtgaagt ctctgaaaag agattagaag
     6001 cgattaaagg atttacagaa ttgggctctg gctttaagat tgcaatgcga gatctttcga
     6061 ttcgtggagc aggaaatctt ttaggaaaat cccagtctgg tttcattgat tctgttggtt
     6121 ttgaattgta ttcgcagtta ttagaggaag ctattgctaa acgaaacggt aatgctaacg
     6181 ctaacacaag aaccaaaggg aatgctgagt tgattttgca aattgatgcc tatcttcctg
     6241 atacttatat ttctgatcaa cgacataaga ttgaaattta caagaaaatt cgtcaaattg
     6301 acaaccgtgt caattatgaa gagttacaag aggagttgat agaccgtttt ggagaatacc
     6361 cagatgtagt agcctatctt ttagagattg gtttggtcaa atcatacttg gacaaggtct
     6421 ttgttcaacg tgtggaaaga aaagataata aaattacaat tcaatttgaa aaagtcactc
     6481 aacgactgtt tttagctcaa gattatttta aagctttatc cgtaacgaac ttaaaagcag
     6541 gcatcgctga gaataaggga ttaatggagc ttgtatttga tgtccaaaat aagaaagatt
     6601 atgaaatttt agaaggtctg ctgatttttg gagaaagttt attagagata aaagagttta
     6661 aggaagaaaa ttccatttga tatttttctt ctataaaata gataaaaata gataaaaatg
     6721 gtacaataat aaattgaggt aataaggatg agattagata aatatttaaa agtatcgcga
     6781 attatcaagc gtcgtacagt cgcaaaggaa gtagcagata aaggtagaat caaggttaat
     6841 ggaatcttgg ccaaaagttc aacggacttg aaagttaatg accaagttga aattcgcttt
     6901 ggcaataagt tgctgcttgt aaaagtacta gagatgaaag atagtacaaa aaaagaagat
     6961 gcagcaggaa tgtatgaaat tatcagtgaa acacgggtag aagaaaatgt ctaaaaatat
     7021 tgtacaattg aataattctt ttattcaaaa tgaataccaa cgtcgtcgct acctgatgaa
     7081 agaacgacaa aaacggaatc gttttatggg gggggtattg attttgatta tgctattatt
     7141 tatcttgcca acttttaatt tggcgcagag ttatcagcaa ttactccaaa gacgtcagca
     7201 attagcagac ttgcaaactc agtatcaaac tttgagtgat gaaaaggata aggaaacagc
     7261 atttgctacc aagttgaaag atgaagatta tgctgctaaa tatacacgag cgaagtacta
     7321 ttattctaag tcgagggaaa aagtttatac gattcctgac ttgcttcaaa ggtgataaaa
     7381 tggaaaattt attagacgta atagagcaat ttttgagttt gtcagatgaa aagctggaag
     7441 aattgactga taaaaatcaa ttattgcgtt tacaagaaga aaaggaaagg aagaatgcgt
     7501 aaattcttaa ttattttgtt gctaccaagt tttttgacca tttcaaaagt cgttagcaca
     7561 gaaaaagaag tcgtctatac ttcgaaagaa atttattacc tttcacaatc tgactttggt
     7621 atttatttta gagaaaaatt aagttctccc atggtttatg gagaggttcc tgtttatgcg
     7681 aatgaagatt tagtagtgga atctgggaaa ttgactccca aaacaagttt tcaaataacc
     7741 gagtggcgct taaataaaca aggaattcca gtatttaagc tatcaaatca tcaatttata
     7801 gctgcggaca aacgattttt atatgatcaa tcagaggtaa ctccaacaat aaaaaaagca
     7861 tggttagaat ctgactttaa actgtacaat agtccttatg atttaaaaga agtgaaatca
     7921 tccttatcag cttattcgca agtatcaatc gacaagacca tgtttgtaga aggaagagaa
     7981 tttctacata ttgatcaggc tggatgggta gctaaagaat caacttctga agaagataat
     8041 cggatgagta aagttcaaga aatgttatct gaaaaatatc agaaagattc tttctctatt
     8101 tatgttaagc aactgactac tggaaaagaa gctggtatca atcaagatga aaagatgtat
     8161 gcagccagcg ttttgaaact ctcctatctc tattatacgc aagaaaaaat aaatgagggt
     8221 ctttatcagt tagatacgac tgtaaaatac gtatctgcag tcaatgattt tccaggttct
     8281 tataaaccag agggaagtgg tagtcttcct aaaaaagaag ataataaaga atattcttta
     8341 aaggatttaa ttacgaaagt atcaaaagaa tctgataatg tagctcataa tctattggga
     8401 tattacattt caaaccaatc tgatgccaca ttcaaatcca agatgtctgc cattatggga
     8461 gatgattggg atccaaaaga aaaattgatt tcttctaaga tggccgggaa gtttatggaa
     8521 gctatttata atcaaaatgg atttgtgcta gagtctttga ctaaaacaga ttttgatagt
     8581 cagcgaattg ccaaaggtgt ttctgttaaa gtagctcata aaattggaga tgcggatgaa
     8641 tttaagcatg atacgggtgt tgtctatgca gattctccat ttattctttc tattttcact
     8701 aagaattctg attatgatac gatttctaag atagccaagg atgtttatga ggttctaaaa
     8761 tgagggaacc agatttttta aatcattttc tcaagaaggg atatttcaaa aagcatgcta
     8821 aggcggttct agctctttct ggtggattag attctatgtt tctatttaag gtattgtcta
     8881 cttatcaaaa agagttagag attgaattga ttctggctca tgtgaatcat aagcagagaa
     8941 ttgaatcaga ttgggaagaa aaggaattaa ggaagttggc tgctgaagca gagcttccta
     9001 tttatatcag caatttttca ggagaatttt cagaagcgcg tgcacgaaat tttcgttatg
     9061 atttttttca agaggtcatg aaaaagacag gtgcgacagc tttagtcact gcccaccatg
     9121 ctgatgatca ggtggaaacg attttgatgc gcttgattcg aggaactcgc ttgcgctatc
     9181 tatcaggaat taaggagaag caagtagtcg gagagataga aatcattcgt cccttcttgc
     9241 attttcagaa aaaagacttt ccatcaattt ttcactttga agatacatca aatcaggaga
     9301 atcattattt tcgaaatcgt attcgaaatt cttacttacc aaaattggaa aaagaaaatc
     9361 ctcgatttag ggatgcaatc ttaggcattg gcaatgaaat tttagattat gatttggcaa
     9421 tagctgaatt atctaacaat attaatgtgg aagatttaca gcagttattt tcttactctg
     9481 agtctacaca aagagtttta cttcaaactt atctgaatcg ttttccagat ttgaatctta
     9541 caaaaactca gtttgctgaa gttcagcaga ttttaaaatc taaaagccag tatcgtcatc
     9601 cgattaaaaa tggctatgaa ttgataaaag agtaccaaca gtttcagatt tgtaaaatca
     9661 gtccgcaggc tgatgaaaag gaagatgaac ttgtgttaca ctatcaaaat caggtagctt
     9721 atcaaggata tttattttcc tttggacttc cattagaagg tgaattaatt caacaaatac
     9781 ctgtttcacg tgaaacatcc atacacattc gtcatcgaaa aacaggagat gttttgattc
     9841 aaaatgggca tagaaaaaaa ctcagacgtt tatttattga tttgaaaatc cctatggaaa
     9901 agagaaactc tgctcttatt attgagcaat ttggtgaaat tgtctcaatt ttgggaattg
     9961 cgaccaataa tttgagtaaa aaaacgaaaa atgatataat gaacactgta ctttatatag
    10021 aaaaaataga taggtaaaaa atgttagaaa acgatattaa aaaagtcctc gtttcacacg
    10081 atgaaattac agaagcagct aaaaaactag gtgctcaatt aactaaagac tatgcaggaa
    10141 aaaatccaat cttagttggg attttaaaag gatctattcc ttttatggct gaattggtca
    10201 aacatattga tacacatatt gaaatggact tcatgatggt ttctagctac catggtggaa
    10261 cagcaagtag tggtgttatc aatattaaac aagatgtgac tcaagatatc aaaggaagac
    10321 atgttctatt tgtagaagat atcattgata caggtcaaac tttgaagaat ttgcgagata
    10381 tgtttaaaga aagagaagca gcttctgtta aaattgcaac cttgttggat aaaccagaag
    10441 gacgtgttgt agaaattgag gtagactata cctgctttac tatcccaaat gagtttgtag
    10501 taggttatgg tttagactac aaagaaaatt atcgtaatct tccttatatt ggagtattga
    10561 aagaggaagt gtattcaaat tagaaagaat aatctttaat gaaaaaacaa aataatggtt
    10621 taattaaaaa tccttttcta tggttattat ttatcttttt ccttgtgaca ggattccagt
    10681 atttctattc tgggaataac tcaggaggaa gtcagcaaat caactatact gagttggtac
    10741 aagaaattac cgatggtaat gtaaaagaat taacttacca accaaatggt agtgttatcg
    10801 aagtttctgg tgtctataaa aatcctaaaa caagtaaaga aggaacaggt attcagtttt
    10861 tcacgccatc tgttactaag gtagagaaat ttaccagcac tattcttcct gcagatacta
    10921 ccgtatcaga attgcaaaaa cttgctactg accataaagc agaagtaact gttaagcatg
    10981 aaagttcaag tggtatatgg attaatctac tcgtatccat tgtgccattt ggaattctat
    11041 tcttcttcct attctctatg atgggaaata tgggaggagg caatggccgt aatccaatga
    11101 gttttggacg tagtaaggct aaagcagcaa ataaagaaga tattaaagta agattttcag
    11161 atgttgctgg agctgaggaa gaaaaacaag aactagttga agttgttgag ttcttaaaag
    11221 atccaaaacg attcacaaaa cttggagccc gtattccagc aggtgttctt ttggagggac
    11281 ctccggggac aggtaagact ttgcttgcta aggcagtcgc tggagaagca ggtgttccat
    11341 tctttagtat ctcaggttct gactttgtag aaatgtttgt cggagttgga gctagtcgtg
    11401 ttcgctctct ttttgaggat gccaaaaaag cagcaccagc tatcatcttt atcgatgaaa
    11461 ttgatgctgt tggacgtcaa cgtggagttg gtctcggcgg aggtaatgac gaacgtgaac
    11521 aaaccttgaa ccaacttttg attgagatgg atggttttga gggaaatgaa gggattatcg
    11581 tcatcgctgc gacaaaccgt tcagatgtac ttgatcctgc ccttttgcgt ccaggacgtt
    11641 ttgatagaaa agtattggtt ggccgtcctg atgttaaagg tcgtgaagca atcttgaaag
    11701 ttcacgctaa gaacaagcct ttagcagaag atgttgattt gaaattagtg gctcaacaaa
    11761 ctccaggctt tgttggtgct gatttagaga atgtcttgaa tgaagcagct ttagttgctg
    11821 ctcgtcgcaa taaatcgata attgatgctt cagatattga tgaagcagaa gatagagtta
    11881 ttgctggacc ttctaagaaa gataagacag tttcacaaaa agaacgagaa ttggttgctt
    11941 accatgaggc aggacatacc attgttggtc tagtcttgtc gaatgctcgc gttgtccata
    12001 aggttacaat tgtaccacgc ggccgtgcag gcggatacat gattgcactt cctaaagagg
    12061 atcaaatgct tctatctaaa gaagatatga aagagcaatt ggctggctta atgggtggac
    12121 gtgtagctga agaaattatc tttaatgtcc aaactacagg agcttcaaac gactttgaac
    12181 aagcgacaca aatggcacgt gcaatggtta cagagtacgg tatgagtgaa aaacttggcc
    12241 cagtacaata tgaaggaaac catgctatgt ttggtgcaca gaatcctcaa aaatcaattt
    12301 cagaacaaac agcttatgaa attgatgaag aggttcgttc attattaaat aaggcacgaa
    12361 ataaagctgc tgaaattatt cagtcaaatc gtgaaactca caagttaatt gcagaagcat
    12421 tattgaaata cgaaacattg gatagtacac aaattaaagc tctttacgaa acaggaaaga
    12481 tgcctgaagc agtagaagag gaatctcatg cactatccta tgatgaagta aagtcaaaaa
    12541 tgaatgacga aa
//
LOCUS       DAVLXF010000056        12501 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLXF010000056 DAVLXF010000000
VERSION     DAVLXF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.056"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..428
                     /locus_tag="VYO12_001462"
     CDS             <1..428
                     /locus_tag="VYO12_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1588052.1"
                     /translation="LANDSGKKKFHAMKAQAIVTSQGRIVSLDITVNYCHDMKLFKMS
                     CRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTVEDKACNHALSKERSKVEN
                     IFAKVKTFKIISTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(480..842)
                     /locus_tag="VYO12_001463"
     CDS             complement(480..842)
                     /locus_tag="VYO12_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588053.1"
                     /translation="MPKTKTLAKLADVILWSFDFANDHAHAFFMDNVEWSHADSYFFS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1196..3076)
                     /locus_tag="VYO12_001464"
     CDS             complement(1196..3076)
                     /locus_tag="VYO12_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1588054.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVTIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RHAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3070..3939)
                     /locus_tag="VYO12_001465"
     CDS             complement(3070..3939)
                     /locus_tag="VYO12_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1588055.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILYKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQTCTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4023..6668)
                     /locus_tag="VYO12_001466"
     CDS             complement(4023..6668)
                     /locus_tag="VYO12_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1588056.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKVLHPTEGYKKMAALILPSYRVEDL
                     DGHPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(6759..8039)
                     /locus_tag="VYO12_001467"
     CDS             complement(6759..8039)
                     /locus_tag="VYO12_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1588057.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFVELDLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8210..10294
                     /locus_tag="VYO12_001468"
     CDS             8210..10294
                     /locus_tag="VYO12_001468"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1588058.1"
                     /translation="MKPLLETIDTRLGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTGQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDVIETGEANRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLKTASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(10334..12013)
                     /locus_tag="VYO12_001469"
     CDS             complement(10334..12013)
                     /locus_tag="VYO12_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1588059.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPAYG
                     NGGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGNCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3434 a         2996 c         2552 g         3519 t
ORIGIN      
        1 aattagcgaa tgattctggt aaaaagaaat ttcacgctat gaaggctcaa gcgattgtca
       61 caagtcaagg gagaattgtt tctttggata tcactgtgaa ctattgtcat gatatgaagt
      121 tgttcaaaat gagttgcaga aatatcggac aagctggtaa aatcttggct gacagtggtt
      181 atcaagggct catgaagata tatcctcaag cacaaactcc acgtaaatcc agcaaactca
      241 agccgctaac agttgaagat aaagcctgta accatgcgct atccaaggag agaagcaagg
      301 ttgagaacat ctttgccaaa gtaaaaacgt ttaaaataat ttcaacaacc tatcgaaatc
      361 atcgtaaacg cttcggatta cgaatgaatt tgagtgctgg tattatcaat catgaactag
      421 gattctagtt ttgcaggaag tctattgaaa tactaagcct acacagactg atttcaattt
      481 taccattctt catagtcaaa accatcataa tctggatatt gttctggcga cgttccaacc
      541 gaacgtacga gataagcttc ttcgtcctct gtctcgattt ctctcagcac ttggcattca
      601 aaacgccatt catcaccgaa gtcgaaaata aacttaaatt tttgtttgac acttaggcta
      661 tccagataga cattttctgt gtaacgttct tcaacatcgt cactaacaaa gctaaaaaag
      721 taagaatctg catgactcca ctcaacatta tccatgaaaa atgcgtgagc atgatcattt
      781 gcaaaatcaa aactccataa aatcacatct gctagtttcg ctaacgtttt tgtttttgga
      841 atctatatat gtcggtagca acctttttta aatttgacgg aaatgaccca agcttctatt
      901 tcttgtttta ctaattttaa gtgtagcata atacctccaa ttgctcatcg atagaattgc
      961 tcttaggaaa cattcacttt ctgctatttt atcataagtt tgtctaactg tagcatttga
     1021 aaccagataa ttcttctgtt tcacgcgaaa caccctgcgc caagcaaggt gttcttgttt
     1081 ttatactcaa tgaaaatcaa agagcaaact aggaagctag ctgcaggctg ctcaaaacac
     1141 tgttttgagg ttgtagatgg aagctgacgt ggtttgaaga gattttcgaa gagtattaag
     1201 tcgtataaat cgtcgacgct gtcgcaatgg tatgccactg gttagctgta gttgctgcga
     1261 agtccttgtc tgcgtagaag tcagtaaatg gtaggatttc aacttccagc tcgtcgatgc
     1321 ggtcaagctg accagccaga taaacctcga tacggctttc tgctcgtttg atgcgttgca
     1381 agagtccgcc catacggatg tcaactgtat ccaaaccaaa gaccttgttt tctttcagcc
     1441 attggtggct aaagagggca tggaagtctt caatttggct tctaagttct ggtaattctt
     1501 gtctggcgat ttgttgtaaa ctttctttat catccgcttg gtaggcatga cgaatgcgtc
     1561 gtcccacatc tactttgcta cttaaaatag cattcaactg ggcctgagtt tcaaagagat
     1621 aagcatagtt tccagctttt tctttaatgt tagcaagcgt ctcagcagcc tgagcgaagt
     1681 gcggtttgtc ctgttcaggt gtcatgtgtt gatcaagaat cggacaaaga atatcctgat
     1741 aaaaaacata gcggttggga ttgataccgc tgagattgcc tggtaggtct ggtaagaggt
     1801 tggcaagatc aatctgcata aaatcctcaa ccgttagacc agtattggtc ttgaagtgcg
     1861 cagacaaact atctaggtca ttgcgatagc tgagttctgc ccagatttgc aagcttggta
     1921 ggatagagaa ctgggcagtt tcaccaccat tgtctcccca accagttacg atgacttctt
     1981 taatctgatt ggcacggcag gctttattag cctcgatagt cactagacgg ctaaaatggt
     2041 tgtgaggtgt aaagccaatc cacttccaag ctccccctgc aaatgcaagg tcatggctaa
     2101 tcttgtgatg attgcggaaa ttacggttgt atttttcctc gctatcctga taataatccc
     2161 agtaaaccag agtcacacgg tctttgagac ggtctaggta gacacgagtt tcctctggaa
     2221 tttccacatc acggtcgtac tggccatccg ctgacatgag tttgaagaac atatcactcc
     2281 acatctggca gtggaaacca tatttgtcag caatatccag cacgcgctcc aagtgttggc
     2341 acatgaggag actacgatcc acaacaccgt tcaaaatcag gtagcgtccc aaaccaacca
     2401 agtgggcttc gtccatcccg atattgacct tgcgagtctt cagtttagac aacgtggcaa
     2461 acatgccatc aatcaggtca taaacctttt cttcgccaat gagaagaatg tcctctacat
     2521 cacggagctc ctggacttcc ttgacacccc atttgacaaa ggccgacaag tgggccaagg
     2581 tctggatgca tggtacaaag gtcatgtcaa actgttgggc ataggcttcg atttcctgca
     2641 actcctctgc tgaataagct ccacggaaat agccaaagta aggctgtcct tcaatctggt
     2701 aagtgtcttc catgtaaagc tcaaaggttg agtagcccat gagagccaag acctcaatca
     2761 tctgcttggc agaagccaca ttcagcaccg catttcgaga acagtcaacc atgtaagcca
     2821 aatcttcgta agctgcttgt tcctcaatct ctactttatc agcttctgct agaactgtta
     2881 ccaacaagga taaggcacga taaagttggt gaggtttgcg gtaggtcaat tgatagtgac
     2941 cttcctcacc ctcgatagag atagaggctt ggtcagactg agtgacagcc acttccacat
     3001 ctggtagaga aatatgacct tttaaaacct caatagcttg cgtttgtttg ggactaagtc
     3061 ctgtaaatct taccattgct tttcctcctg tagccagttg acaagggcac cgtagagatt
     3121 ggcatctgcg tgataggtgc aggtctggat aactggtgcg accgtgtatt cttcgtaggc
     3181 atcgacaaag tcttcaacag ccttcttaac accttggata aaatctggat tttgactgat
     3241 agagccaccc agactgatga catctggatc gatcagatac tggatattga gcaagccttg
     3301 cgtcagattg cggttcatgc gctcaatggc ttcttgacaa agagcattac cagctgcggc
     3361 ctcttggtaa atcttgcgac cgtcccaatc agtatgacca gatttttcaa tcacgtatcg
     3421 taccatattc ccagttgatg ctagttgcga ccagttatta agtttttcag caggggcaag
     3481 ggttgtcatg tagccaaatt ctccacccag accgtggcga cctcgatgaa gtctaccatt
     3541 gataatcatg gctccgccaa tccctgtccc aatcacgaca caggctgcat tttcaagctc
     3601 tggatgagct agtagttcac tgagtccaac gcagttggca tcattttcta aatggacagg
     3661 tagctgataa gagctaagcg cctcatacca agaaaagcca tggatgtagg gcaccgcact
     3721 gaagccatca atcacacctg tctcttgatt gaccgcacct ggaacgctca tagcaatccc
     3781 actgtaatcc tgttctgaca agcgttgatc tagccacgct agtaaatcct ccaagttttc
     3841 aggcgttgaa atacttgtct tatacagtat tttcccatca ggagtcagac tggcaaactt
     3901 aatcccagtc cctccgatat caatcgttgc aatggtcatt gtttttacca taaaaagggg
     3961 cgaatcagca gttagttctt actttttcgc ccctcctttc ttttgatgtt tactttttga
     4021 aattaaattt cttctttttt gatgaattct gtacgaatct cttgtggagc caatagtcct
     4081 gaatgaactg gatatggtcg ctcaagtaag tcaagaatgg tttgttgatg ttctgatatg
     4141 cgcacatttt cttgactcat attatagtaa cggaggacat agccttcttc attttccgcc
     4201 actttaaagg ctgttggaca aacttgcggt atgctgagaa cagaatggct caagaggcta
     4261 ccagtcgcaa ccacgcttcc ttcctgtcta gcaagctgaa ggctggtaaa cggtgtctgc
     4321 aaggctttgg cacgacgata ggctgagaag cgttcttggg cttggtggca ttcaagtgca
     4381 aactcgactt caaactcacg caagcattgt gcttctggcg ttgggaagta gccccagtca
     4441 cctagctcac ctgatgcacg caaaatggtc acggcaatgg tgttatcccc aaggatttcg
     4501 tattcattca atcccttatt ggatacagtc accccttttt catcgtcata cagactgaca
     4561 aaagcttgtt ggtgttgagg attttcagga ttttcccatg aagcagctgg tttgtttggt
     4621 cgtgtcacca cctcatagat actttcagaa tcattgcttg gacgcgtgtt atgagtcttg
     4681 accaagagac ggatacggtg atccttggca gtgttagtaa agcgagtctt gaagcggatt
     4741 tgtggattgt caacgaagac agtcaactca gtttccagag gaatgtttgt caattcttct
     4801 gaccgtccag cctcacgctt cataaactcg atgatacctt gttgctcttc ttctagcttt
     4861 tcatccgcgc tgacaggcac ggtcaattca tgtttgagca agattttagc atagcaagct
     4921 gtgttttcca agacctcgtg gcccttaagt tctgcaaaga ttggctctgt tccttttggt
     4981 tggaaataga tatactcgtt tccgatgtcc ccacggtctt caaagcggat aaagtcttca
     5041 taggcttcgt gagttgtctt gtcatagact gtgatgttgt catccacact caccgttacg
     5101 aatggtgtat caatcactcc gttttggtaa ataccctcac ggtgttcttg ttttccttcc
     5161 agcaattgga aggttgtcca agaaagcggc gctaggtgaa ctggaatggt cacgcgcact
     5221 tgacgagcaa tacgagcttg gcggaacttg tcttttggta aatcatactc aaaattagct
     5281 ccgaggtctt cgattgtagc ctctacagga tgaccatcca agtcctccac acggtaactt
     5341 ggcaagataa gagcagccat ctttttgtag ccttctgttg ggtgcaatac cttgaaatca
     5401 caagtcgcca catcaatcac tgtgctgaca gtatcgacct tatcatgcaa gcctgtgtta
     5461 atgacagtaa agagatagtc actttgagcc ttatccgtag caattttacc cttccactcg
     5521 ttgagcaagt tacttttaac aaagtttcct acttggttga ccttggcaaa acgcgtttcc
     5581 atctcgcggt gaacttcgtc cacgctacag ccacagatac tatcatgtgg cgcattctgc
     5641 aaaagtgttt tccaagcata ggtcaactgg tccttgtggt tgtgtccacc agtgataata
     5701 gtcaagggtt ctaccacttg ctctaggagg ttgctatttt cttggaaggc ttgttttagg
     5761 taaatgcggg atgaagaagt gttggcaagt gtgtaccagc catctgtttc ctgactggtc
     5821 aactcgcctg taacagttga taagtgttca ggaagcgcac cttctacagc ttgaacatat
     5881 tcatcaaaag aactatgaac aaagattaca tccgggaaga gttcatttgc cacacgaatg
     5941 gcttcgctca gatttttctg tacaggctgg tggtcacagc cgttcatcat caaccattgg
     6001 ttggtcgaag cgtaggcacg cacatctgac aatttttgtt tccagaaggt caaggcctca
     6061 tctttgtcaa ctgggatttc attcccatta ctgtaccagt tggcaaagag aatacctaaa
     6121 acacgactac catccacacc ctgccagtac atttctgaaa actgggatgt aaactgctca
     6181 tcttcaagga cttggttgtc aaatccaatc ggcttcacac cacgaccaaa ggccgccacg
     6241 tgaatgcctg atttttgaag aatttgaggc gcttgtccca tatttccaaa ggtatctgga
     6301 aagtagccaa tctgggttga tttaccccat ttggcagctt cttgttgacc aatcaaggta
     6361 ttgcggacat tggcttcact ggagatcaag tagtcatcct gcaagatgta aaagggacca
     6421 attttaagtt tgccctcgtc aatgtagcgt tggaccttgt cgcgattttc agggcgaatt
     6481 tgtaagtagt catcaaggac aatagtttgt ccatccaagt ggaaactctt gaactcaggg
     6541 tcattttcaa agagatcaaa gagattgtca aacaattcca ccaactgcat acgatggctt
     6601 tcaaaaggca agtaccactc acggtcccag tgactatgtg agataatatg tacaacaaca
     6661 ttttccatga agtaaaacct cattctaaat ttaaatttta acgttttaaa tgtaaacttg
     6721 tgattctgat aagaatcggt tgagctaaag cgagcccctt agcgaatatc caagtaatcc
     6781 aagaccaact cacagaacat catgttagcc caggagaacc attcacgaga gtagagggtc
     6841 ggatcatcta cgtggaagct ttcgtgcatg acacctgtac caccatcgca ggcaaccagc
     6901 tgatccagca agaatttttt ctctgcctta tctcttgttg tcaatccttg gatagaaagg
     6961 gcgattggcc agatatagcg atagaaggta tgagaacttc cgagaccgct agcgtattct
     7021 ccttggtaga agtatggatt ttcagagctc agaatggtac ggcgagtggc ttgatacact
     7081 tcatcatcga tcgaacagta gcccagatag ggcgcagcta gtagacttgg tacatttgga
     7141 tcatccatga tgctggcatt tcctaggcca tctacttcaa aagcgtaaat cttttcgccc
     7201 ttgctgttgg tggtgtaagc gtagttttcg attccttctt ggatttcatc ctgaagacgc
     7261 ttggcatcag caataacact ctggctatca gctaggtcta attcgacaaa gatttcttgc
     7321 acataaccca agactactac ggcaaacata ttagacggaa tcaagtaact atactgacag
     7381 caatcgtcac tcggacgaaa agctgaccaa gtcatacctg tcactgcaaa gtcaggtcca
     7441 aagccatcat ttaccaaggt gtcttcctta cggtccgtat ctcggacaaa acgataagga
     7501 gagttcttgt ggtcttgttc aactgtccat agatggagaa tttccttagt cgctgcgaca
     7561 aaagtctcat caaactgacc agtctcgcca gtctctttcc agaggagata agccaactgc
     7621 aaaggatagc aaagtgaatc cacctcatac ttgcgctccc agatccagcc gttaaggtct
     7681 gtgtggtcag tctcgtggtg ccctttccag ttcttctcaa tgttgaagga gttagcatag
     7741 ggatccttga gtaccaaggt catctgacgt ttgaccaaac ctgcaatggt ctgacgcagg
     7801 agggcatctc ttttagctac atgaaggtag ggtctgagtt gggctgtcga atctcgaagc
     7861 cacatggcag gaatatcccc agtcaagaca aaagttgaac catcttctaa gatttcaacc
     7921 gtattgtcca aggtatcggt gtagcaacgc tcgaaaacat ccacccactc tggatggtcc
     7981 ttagcccgct ctgctacttc atctagccat tctctaacaa tttctttcga ataaatcatc
     8041 gtgcaattcc ctctttcaaa caagtttcat tttcagtata tagcttttga gacagaaaat
     8101 acatacacaa atgatagtca tttttctaca aaatagttat ctttgaaact ccttttctaa
     8161 aggccctctt tttctgatat aatgtagaaa aacagctaaa ggaaagacta tgaaaccact
     8221 acttgaaacc atcgataccc gcttgggaac taccaacaag catgcctttt ctcggggaaa
     8281 taccctgcca tacacaggcg tgccttttgg gatgaattac tttgtgcccc agaccagtga
     8341 ccaggatgga tcttggttct tcgatccgca tctgcctatc tttcagggga ttcgattaac
     8401 tcaccagccc agtccttgga ttggggacta ctcttggctc cttctgacac ctgtcacagg
     8461 tcagcttggg ggagacagtc tctttcaccg ccaatcttcc tatgataggg ataaggcctc
     8521 tttccaacct cattatctaa agattttctc cctgcgctat caaattgaaa cccagctcac
     8581 accgacttgc tacggtgctt ctattcgttt gaagcaaaag caaggcaaag ccctctccct
     8641 ctatcttcac gcagcagatg aactgacagt agagcaagta gataagcgaa ctcttgccct
     8701 gcgacaagaa ggtaaaactg aaaccaacaa aaattcgcta acgatgttca ctgccctgca
     8761 aatgaacacg gatattcttg ctatcagcca agaagctgga gactggcgaa ttgacttagc
     8821 aagtagtcaa accgagatgc aactagccac ttctttcatc tctccttctc aagctctaat
     8881 caatctacct caagaagatt ttgatagctg taaagcaagt gcccaagcgg attgggaaaa
     8941 tctcctccat cgttttgatg ttatagagac aggagaggct aaccgaacct tctttgacca
     9001 ctgtctctac agactcttcc tcttcccaca gactttttat gaggttgatg agtcagggca
     9061 agccatccac atggatctgg ctactggtac tgtcaagcct ggtgtcctct ttagcaacaa
     9121 tggtttctgg gataccttcc gcaccacctt ccccctcttt gcccttatca taccggagca
     9181 ctatcaacgc tttttagaag gtttcctcaa tagctaccgc gatactggtt tccttccaaa
     9241 atggctggct ccagatgaac ggggtatgat gccaggtaca cttttagacg gtattatcgc
     9301 agatagcgcc tgcaaggaca tggcccccga cctagaagga gaactcttcc aagccatgct
     9361 caaaacagcc agcaaagccg accctctcgg catcaatggc cgccacggac tagcccaata
     9421 ccaagaacta ggttacctct ctaccgacca ccacgaaagt gttagccata ctctagacta
     9481 tgcctatagt gacttttgta tcgccaactg tgccaaaaaa ctaaaaaaca tagaaatcgc
     9541 tgaaacctac aaggctgctt cacaaaatta ccgccagcta tttgacgctg agacaggtta
     9601 catgcgagca cgagacaatc aaggaaactt tcaccctgac ttctctcctt atagttgggg
     9661 gcgagactac gctgaatgct ctgccattca agctacttta ggcgttctcc acgacatccc
     9721 tggcttaatc caactgatgg gtggaaaaga aacctttagc aactatcttt tgaaagcctg
     9781 tcaagatgct cccctctttg aaacaacagg ctatggttac gaaattcacg aaatgagcga
     9841 gatggctact gctccttttg gacaaatagc catttccaac cagcctagtt tccacattcc
     9901 ttatctcttc cgctacagcg attaccctga ctacactgcc cttcttatca agacgctccg
     9961 tcagaaagct tttcacccaa gttgggaagc ctatcctgga gatgaagaca atggtagtct
    10021 ctctgcttgg tacatctggt cagctctcgg attttatcca acctgtccag gaaaaccaag
    10081 ctatgatctc ggaattcctc tctttgacca tctacgagtc tacctagcta aagaaaataa
    10141 atggctagat attcatactg agcaaaacca cagccatttt aactttgtca aagaatgccg
    10201 actggacaaa acactcgtat caactattca acaccaagac ctcctaaaag ctgaacaact
    10261 aactttcacc ctcagctggc taccaaatca ctaataacca aaagaacctt tgcatcgcgc
    10321 aaaggttctt cttttatgaa acttggactt gaagttgatc cacaagctga ccatccaccg
    10381 ttaggttcag atagaaatcc aaggttttat ctcctgcgaa atacagggtt ggtagatgca
    10441 aatctttttt gctttcccct gcttggaatt gaaggacttg aatctcatcc tgataggcga
    10501 caccatggac acctgtcccc ggttgaatcg aaatcttagc ctctaaagag gagctacttt
    10561 cactacgttc aatcctaaaa tgaagagtct ctccctttgc cacagctagg cttttttctg
    10621 caaatgctag cccctgaaca gctactgttt gtgataacct cggagtttta tagagtgaaa
    10681 tcttggtcaa caaaggcaaa gcctgtgatt ctgtaatgac tacacgtatc ttctgtgctt
    10741 caacaactgc tcctcgcaag aggcgcttat gaccaactgt gaagcccctg ccaaattcct
    10801 gccagacacc atccacctct acttgcacat ggaaagcaga aatacgttgg cctaacttca
    10861 aatcttctct caactcaatt acatcaaaag ttttaggaga acctaagtcg agttctaact
    10921 ggatgggcaa gtctgcatcg cttgcccaag agctggtctc aaggccgtct gtcaaatggc
    10981 gacaagcaaa gtctgcggaa agagctggac cagatacctc agctcccaga gccaaatctt
    11041 ctttatagag ctcattgcga taggtcgcaa attcataaag tcgttcaata tcctttgcat
    11101 caaagagccc agcttgattc ggcggaatat taagcaagag tggagttcct cgccctactg
    11161 agtgaaagta gatttcgacc aactcctcga gagacttagg atcctgatcc tcatggtaga
    11221 accagcctgg acggatggaa acatctgcct ctccgattga aaaaatcgtg cccgagggat
    11281 ccccgtgctg aagatagtcc agctctgctt ctgttcctag tttatcagga ttcacctttt
    11341 gccacagtgg atcacctgca taccctcgtt cattgccaat ccatcggata ctggtgcctt
    11401 ctgttgaaaa aatcaagcaa ttgccctgca ggtcacgaat ggtttcaaac catttttcaa
    11461 attcataatt aaccttttgc gcgccctctc ctctggcacc atccatccaa acctcagcaa
    11521 atttccctcc atttccatag gcaggatttg ataagatttc cttcagctga gccagataat
    11581 aggcattgta gtccgcttct cggtccacat gatagagggg actatgggca tcccacggtg
    11641 acaggtagac ccccatatcc atatcaaact ctgtggcagc ttgggatact tcaaggagca
    11701 agtcgccctt tcctctcctc caaggactga ccttaaccga ataatctgtg tgagctgtcg
    11761 gataaaggac aaagccatcg tggtgcttga ccaccaaaat caactttttg aagcccgttt
    11821 ccttgagcac acgaacccac tcacgcgcat ccaactgact cgggttaaag cgctcaggat
    11881 cctcctgtcc agtcccccat tcttggtcat aaaaggtatt aggaccgaag tggataaaag
    11941 ctgctagttc atctcccaga taagctagct gagtctgact tggtaacggt ccatgcggtt
    12001 tgattttctt catcgttata tatctccttc tttagcaact tctcttgttc acaaggttat
    12061 ccacaggttg tggataataa aaaatgatct aaccgccagc tctttccaac tccaactggt
    12121 aggctcagga tttttctcct caatattccc tcttcaaaca gctctgtcat atggttctgc
    12181 taacacctct cagaatgcta ctctccataa ttattctcag gcttctttca ggtcattgtg
    12241 tgaagaccat tcactctcca gacttttcca cagattgtga ataagtgatt attctaaact
    12301 atcatctcgt cgactataac aaaaaataaa aagagggtca ataagataat tatctcttcc
    12361 taaatactca gaaaagattc tataatttgt acaattgaac aagaaatgct tctccaaaat
    12421 ctatcttcaa aagaatgttg catcttttta tcctttcaca caaacatttc tctttataca
    12481 gatccaagtg ataaccagat g
//
LOCUS       DAVLXF010000057        12196 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLXF010000057 DAVLXF010000000
VERSION     DAVLXF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.057"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(431..1624)
                     /locus_tag="VYO12_001470"
     CDS             complement(431..1624)
                     /locus_tag="VYO12_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1588060.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(1762..3204)
                     /gene="gtfA"
                     /locus_tag="VYO12_001471"
     CDS             complement(1762..3204)
                     /gene="gtfA"
                     /locus_tag="VYO12_001471"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1588061.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTMLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(3504..4337)
                     /locus_tag="VYO12_001472"
     CDS             complement(3504..4337)
                     /locus_tag="VYO12_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1588062.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(4351..5217)
                     /locus_tag="VYO12_001473"
     CDS             complement(4351..5217)
                     /locus_tag="VYO12_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1588063.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAAFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(5231..6490)
                     /locus_tag="VYO12_001474"
     CDS             complement(5231..6490)
                     /locus_tag="VYO12_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1588064.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(6582..8744)
                     /locus_tag="VYO12_001475"
     CDS             complement(6582..8744)
                     /locus_tag="VYO12_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1588065.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHITLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            8852..9712
                     /locus_tag="VYO12_001476"
     CDS             8852..9712
                     /locus_tag="VYO12_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1588066.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIS
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQSPSHTRKEYS
                     QYQLVRKATL"
     gene            9709..10644
                     /locus_tag="VYO12_001477"
     CDS             9709..10644
                     /locus_tag="VYO12_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1588067.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEINSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(10770..10857)
                     /locus_tag="VYO12_001478"
     tRNA            complement(10770..10857)
                     /locus_tag="VYO12_001478"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10821..10823),aa:Ser,seq:gct)
     gene            complement(10864..10937)
                     /locus_tag="VYO12_001479"
     tRNA            complement(10864..10937)
                     /locus_tag="VYO12_001479"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10901..10903),aa:Ile,seq:gat)
     gene            complement(10973..11043)
                     /locus_tag="VYO12_001480"
     tRNA            complement(10973..11043)
                     /locus_tag="VYO12_001480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11009..11011),aa:Gly,seq:tcc)
     gene            complement(11063..11135)
                     /locus_tag="VYO12_001481"
     tRNA            complement(11063..11135)
                     /locus_tag="VYO12_001481"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11100..11102),aa:Phe,seq:gaa)
     gene            complement(11139..11212)
                     /locus_tag="VYO12_001482"
     tRNA            complement(11139..11212)
                     /locus_tag="VYO12_001482"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11176..11178),aa:Met,seq:cat)
     gene            complement(11223..11312)
                     /locus_tag="VYO12_001483"
     tRNA            complement(11223..11312)
                     /locus_tag="VYO12_001483"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11274..11276),aa:Ser,seq:tga)
     gene            complement(11323..11396)
                     /locus_tag="VYO12_001484"
     tRNA            complement(11323..11396)
                     /locus_tag="VYO12_001484"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11360..11362),aa:Ile,seq:cat)
     gene            complement(11410..11483)
                     /locus_tag="VYO12_001485"
     tRNA            complement(11410..11483)
                     /locus_tag="VYO12_001485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11447..11449),aa:Met,seq:cat)
     gene            complement(11489..11562)
                     /locus_tag="VYO12_001486"
     tRNA            complement(11489..11562)
                     /locus_tag="VYO12_001486"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11526..11528),aa:Pro,seq:tgg)
     gene            complement(11578..11651)
                     /locus_tag="VYO12_001487"
     tRNA            complement(11578..11651)
                     /locus_tag="VYO12_001487"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11615..11617),aa:Arg,seq:acg)
     gene            complement(11661..11746)
                     /locus_tag="VYO12_001488"
     tRNA            complement(11661..11746)
                     /locus_tag="VYO12_001488"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11710..11712),aa:Leu,seq:taa)
     gene            complement(11754..11825)
                     /locus_tag="VYO12_001489"
     tRNA            complement(11754..11825)
                     /locus_tag="VYO12_001489"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11791..11793),aa:Gly,seq:gcc)
     gene            complement(11848..11920)
                     /locus_tag="VYO12_001490"
     tRNA            complement(11848..11920)
                     /locus_tag="VYO12_001490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11885..11887),aa:Thr,seq:tgt)
     gene            complement(11939..12020)
                     /locus_tag="VYO12_001491"
     tRNA            complement(11939..12020)
                     /locus_tag="VYO12_001491"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11984..11986),aa:Leu,seq:tag)
     gene            complement(12026..12098)
                     /locus_tag="VYO12_001492"
     tRNA            complement(12026..12098)
                     /locus_tag="VYO12_001492"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12063..12065),aa:Lys,seq:ttt)
     gene            complement(12123..12195)
                     /locus_tag="VYO12_001493"
     tRNA            complement(12123..12195)
                     /locus_tag="VYO12_001493"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12160..12162),aa:Asp,seq:gtc)
BASE COUNT         3873 a         2690 c         2175 g         3458 t
ORIGIN      
        1 aaattaaatt ataacaatat tgtagaagta tcattctagt attcaagatt cagtttacta
       61 tgtcttttca caccaacctt attccgaatt caattacttt cgtgatttac acatatagat
      121 taagactatc ttttatactt taaaatttct cgctaccttg tccactatat gctcctcgct
      181 atcacgtttc tattcatagc ctacgatttc actattgctt tctctgacaa ttcttatttc
      241 ctgcgtcaga cttaaaacga tctatcccca gaccatttta atccgctacc tcacgatagt
      301 caggcttggg gagcgctatt gtattcaccg gtagtggatc cctacagagg acttacacct
      361 cagatgcacg acatgcccat cgtataaaaa aatctcctac ccaaggtaga agatttcaaa
      421 cttataaaac ttaatccgtc atgtccgata ccaacattcg atgctccaat ggaatactgc
      481 acataactag caagaaaata aagcctgact gaatccagaa gagagccaag tcaaaaattc
      541 cgtgcacagc aaccactgta aggaaagata gataaaggcc gataatcgga cgtttccccg
      601 actcctgact catatccatc atcaagcgaa caggagcaac agaagacaaa actaataaaa
      661 tggtacccac aattccgtaa ctcagaatcg tatcaatata aagactgtgg gcatgttcat
      721 gataaggagc atgtatccga ggataagagt gcatataggt caatggccct tcaccccaaa
      781 aaggattttg cttaaacaag gccatcccag catcccagat agaaatgcgt tcttccatag
      841 aagagtctaa agtacccatt cgaactccca aatcactaga aaagaggaaa ctcaaaccaa
      901 tcgcgaagac cccaatacta agccaaaagg ccttccagtt tttaatagtc gtaaagagat
      961 agataattgc tccagcgata atagcaggaa aggcagttcg attttgagta aagttcaaac
     1021 caaagagatt aacaaagcct gcaatcacac agaatacttt caaccaattc aacttggtcg
     1081 ttgtaaacag atagaaagca atcataatac agaaacaaca aataattcca taataattag
     1141 gattaaagaa ggtcacttct gcccggttct gatgccacac ctgcatattg ggtgaaagaa
     1201 aagcatagtt aaatttcttc acaatttgga aatgttctaa actggcaaaa gcagctgaca
     1261 agacactacc aaacaagacg aactgcaaaa tcaatcgaaa gaatttatgg gataaaatcg
     1321 actgatagtg caaaaagaaa atagtaaata gaaacattcc tactgaagcc acaagaccca
     1381 tccaattttg tgcaagaatg gatataacag tactatagct aagaaaaaga agcagcatcg
     1441 gatgctcccc cattttctga agaatacttt tcatgtctcc tgtaaaaatc aaactgataa
     1501 tatataaaca gagtacaact acaaaaagat aaaagggtaa aaagatactt aggataattc
     1561 ccaataaaat cagctcttta ctagacaacc ccttcagctt ttcaataaag cctattgatt
     1621 tcaaaatgaa tcctttctct ccaaatcagc tgattcagat aatagtaagc tatcctatat
     1681 tgtaccactt ttttagcaat ttgaaaacaa aggaaacgtt ttccaaaata aaaaccctat
     1741 tttatccacc atatcaaggc ttcaaaatga tacttcaact ccattctcaa ttacccgata
     1801 agtctgattt tgcaaatcaa tttctactac tgctgttacg gacttatctt tattttgacg
     1861 tttgattaca atgctgtgag ctgttggtgt ctctatctca gtagtccctt ctagatcaaa
     1921 ggcttctgaa cggttacgga aagaaaatag attgagaagg gccttcacaa caggtcgttg
     1981 cacttctttt gctatttcct cgttgctata gtaatgacga ttaatatttc gaccttcttt
     2041 agtttcttct aataatttca agtcattctt gcctgctaat agacccacat agtaaacctg
     2101 aggaatacct ggggcaaaag cttgaattag acgagcgaga aaatacttga catcatcatc
     2161 tccaagcgct gaatagtagg ttgaattgat ttggtagata tctaagttgt tatactcggc
     2221 actagagtac ttacgtttga cattggctcc aaccttatag agttcatttg aagcatagtc
     2281 aatctcctca tcggtcagga tatccttgac atctactact ccaatcccat catgggtatc
     2341 tagcatcgta aattgcttca tcgggctcat ctttaaccac ttagccaaac gctctgttct
     2401 ggaactgtaa agagtataaa gtgtcaccat tggaagagca aaatcataaa catagtaatc
     2461 atggtctgct attttaaact gaatcgaata gtgttcatga atctcaggta aaagctctgt
     2521 cccatactca gcagcgatat ctcgaacttt gtccaataaa tcccaaatat ctggttccac
     2581 aaagaaatca ttagtatcca atttcttcac tgcataagca aaggcatcta gacgaatcaa
     2641 atcacaccca ttacttgcca agtgctgaat ggtcttacgg ataaattcca tagttacttc
     2701 tttggtcaca tcaagatcaa tctgctcctc accaaaggta ttccacaaat gttccactga
     2761 accatcttca aacacaatct cttgctttgg tgcacgatcc ttacgcttgt aaattaaatc
     2821 tacatcagac tgtgtcggac ggttttctgg ccaaaactta tcccagttta aaaagagagc
     2881 tttaaattca ctggcttcat gtttttcttg atagtcctta taatacttgg attgacgaga
     2941 aatatgatta atcataaaat caaacataag ataatatttc tcacctaaac gcttaacatc
     3001 ctcccaatca ccaaaagctg agtccacttc gtcgtagtca actggcgcaa atccacgatc
     3061 acctgttgat gggaaaaatg gtaaaaggtg aactcctcca atagcatctc caaaatgctc
     3121 ttccaaatta tcatataagt ctttaagatt atttccaagg ctatcagaat aggtaatcaa
     3181 catggtttta ttttgaattg gcatcattac tctccttttt ctaattgaag ccaagtctca
     3241 tatgatctgg cttcataaat aaaattcatt ttaaatctct atttatcatc aaactcgtac
     3301 taatatagac tgtgataaac aaagtactac tttcttgttt tctgcataga attatcaaca
     3361 agctaaactc ttcctctgtg tcaaagacta tagattccat gagctcttcg aaaatctctt
     3421 caaaccacgt cagcttcacc ttgccgtact caagtacagc ctgcggctag cttcctagtt
     3481 tgctctttga ttttcattga gtattacttc actgccccgt tgctcattcc tgaaatgata
     3541 tggcgttgga agaagagata gacaatggtg atactgataa tgccgaccac gtaagaggca
     3601 aagcttggtc cgtagtcgtt gaaatattgg cctgcgtagt tgtattggaa caaaggcaga
     3661 gtccacattt tggaatcccg gttcaagaca aggagtggca acatgaagtc attccagaac
     3721 caaagggcat tgataatcat ggttgtcgca tgcatcggtt tcatcattgg gaagatgatg
     3781 cggaaatagg ttgtaaattg attagcccca tcgatctctg ctgcttcatc cagactttct
     3841 ggaatcgaga ttttgatata gccaacatag agaaagaggg tctgtggaat cgcataggtc
     3901 aagtagagca agatcaaacc aaaggtatta gccaaaccga gtttactcat cataaccgta
     3961 atcggaatca tgatgacttg gaaaggtacg aagattccga ggattaagag ggtatacatg
     4021 atggtaaagg cttttctttt actcatattg cgagcgatgg agtaggctgc catagggata
     4081 aagatcatta ctgcaagtaa agacaagaca gtgatgacga cagagttcca ataatagcct
     4141 ccaatcccat cagctaagag acggctaaag ttgtcccatg tgaagttggt tggaaagcca
     4201 aagaaattat ctacaatatc cttagtgggt ttgaaggaac taaagagggt agcaaggagc
     4261 ggcactaaaa tcagaaccga tcctagaatc aatagaatgt atttgccaat cagggctttt
     4321 ctttcatctt gtttcatcat gcttctcctc ttaaatttca aatttcttag atactctcaa
     4381 ttggatgatc gaaatcacta caattaagag gaacaagatt acggcaatgg cattggcata
     4441 accgaattgg ttgtttttaa aggcatagtt ataaaccaag agcccaagtg aggttgtggc
     4501 attgtttgga ccaccaccgg tcatggcaaa gacttggtca aaggcagtca gcccaccttt
     4561 tagggctagg ataaagacca tagagacact tggtagcaag taaggcaatt caatgttcca
     4621 gaaaacttgc ttgctagtcg caccatcaat ccttgctgcc tctgtaatct cagttggaat
     4681 agattgcaaa ccagctagga agatgatgat gggcatagcc accccttgcc aaagaaggac
     4741 aaaggcagcc gcaaagattg ctccccactt agtccctaaa agactggttt ggagaaattc
     4801 aatatgaagg gcattcccaa tcgctggaag accgtagttg aagacttgct tgaagatcaa
     4861 agccactgtc aaaccagata aaacagctgg gaagaagaac caagcacgga agaaggtttg
     4921 gcctttgatt ttagagttca agacacgcgc aatgaagatc ccgagtgcaa tctcaccaac
     4981 caccatggca atcgtaatga ttgcggtaaa gccaatcgca ttcatgaatt ttggatccat
     5041 gaagaggagc ttaaagttgt ttaagccgac aaatttgtag ttataagtca atcctgtcca
     5101 gttggtaaaa ctgtaaaagg ctccttgaaa catcggcaca tagaagaaaa ttgcttgtaa
     5161 caagaggggg atgaccacaa aagcccatgc ccaatatttt tgtaatactt ttttcatagt
     5221 ctctctactc ctaatccaca tccgctttca tcgggttaaa gaaggcattc aaatcattga
     5281 ccatgccttg tttatcaccg gtcaagacat agttcatggt caaggtatgg aagtctgctt
     5341 cactggtcca gtattgttgc aaccagacca agtgacgatc cgtaaaggca tattcggtca
     5401 taccagcaag cggtgaatct tctcctgctt gtttgacccc ttcgatcgct gttggagatc
     5461 cgtccacatc gtagtatttt tgcatgactt ctggacgggt catatattcc acaaaggcat
     5521 tggcttcttt tggatgtttg gtggtggctg agatagacca tgccaagtct cccgcaccaa
     5581 cggttaagct ttgtcctttt tcttttcctg gaatcatgaa ggtcccaatc ttaaagttcg
     5641 gtttttgttc attaatcgct gtgatcgccc aagacccatt tggtgtcatg aggacatccc
     5701 cacgtgcgaa ggctccgata acatcggtat agccagcacc ttcccagttc ttttgcttag
     5761 agccattgat gcgaaggatg tccatgacct tgatatcatc tttcataatc ggatccgaca
     5821 atttaatggc atttggttga gaataacgaa ggtattgatt tgcttctttt cctccacctg
     5881 ttgctgtcgc aaaggctaat tgattgtaac cattgagtgt ccaagcatct gcacctgcaa
     5941 ttccaaatgg tgtttgtcct ttagcaacga tatctttgac taactgttca aattcatccc
     6001 aggtttcagg aaccttcaag cccagttctt cgaatttatc tttgttgtag taaattccat
     6061 aagcattagc tgtaaaagga acgttgtaaa ctttttcgtt tacagcatat ttttcagcgt
     6121 agccattttt cacgcgtttc aggtagtctt tgttgctcaa atcttcaaaa acacctgctt
     6181 ttgcccattc ttgcagttcg atggactgtg ggtaaatatt gaccacatca ggcacatctc
     6241 ctgcgagaac gcgtgtcttc aatacttcac cagcatttgg tacattgacg actttgacct
     6301 tgatcttagg gttttccttc tcaaaatcac gagtgatttc ttccaaggtt ttggtcattt
     6361 cttttttctg gttgaaatac tcgatggtca ctgtgccatc cgcagattta ccatagttgg
     6421 agcaagcgcc gagcccaaac aaagctaaac ctgtagttgc aagaagtccg atttttttat
     6481 accattccat tagaaagcct cctttataaa tttatacacc cttattgaac tgcaccccaa
     6541 aagttagaca gaataaatct aacttttggg gtcagtacat atcatagttt tctaaaaata
     6601 tactgtctac tcaaaaaatc tccttgggat aagataacag ttaagcccgc atacattagt
     6661 tctgcacctg agtaaacttc gccattttcc tgtaatttat atagtccctc ttcatccaaa
     6721 tcttttaatt ttaaagttgt ttccatggtc tctacaacag ataaaacgcg aacgtaggtt
     6781 acaatcgttt gatttccgta attaaattgt acagctgctt cattggatgc agtatcagga
     6841 ttaattagtc tatactgctg tcctaactga actactggtc gtaattcttt atacaagttc
     6901 acctgattag caatcgtagc tttctcttca tctgataaat ttgtcaaatc aagctcatag
     6961 cccaaatttc ccatcattgc tacatggcca cgtgtttcta atggtgtcat tcgtcccatc
     7021 tgatgattcg gtactgctga cacatgagcc cccatagaaa tggttggata gagataggat
     7081 gaaccgtatt gaattggtaa acgtgcaatg gcatcagtat tatcactagc ccagacttgt
     7141 gggaaatagc gtatcatacc aagatcattt cgtccaccac caccagagca ggactcaaag
     7201 agaatatggc tgtgcttctc tgtcagataa gaaacgagtt cgtaaagtcc cagcatgtac
     7261 tgatgagatt gcatctgtgt ctctagataa gttaatccat tccctagctt agtgatattg
     7321 cggttcatat cccatttaat gtaatcaatc tcatgataag ataggagttg atctaagaca
     7381 cttttcaagt attctactac ctgaggattg gcaagattaa gtactaattg attccgagaa
     7441 taagtatgct cataatcagg aacctgaata gcccagtcag gatgttgacg atacaaatca
     7501 ctatctacag aaatcatttc gggttctaac caaagtccaa actgcaaacc tctttcatgg
     7561 atagctgaaa tcagactttc tagacttcca cccagttttt cctcattaac aacccaatca
     7621 cctaaagcac gattatcatc aaaacgattg ccaaaccaac catcatctaa tacaaaaagt
     7681 tcaatgccaa ctttcttagc ttcatctgct aactctaaca gtttttctct ctgaaagtca
     7741 aagtaagtag cttcccagtt attgattaga attggacgtt cttttttaga aaattcactt
     7801 agagtaatgt gcttcagtac aaaattctga ctttcatgac taataccagt taatccctga
     7861 tctgaatgag tcactaaagc taccggtgtt tcaaagtatt cctcaggggc taacttccaa
     7921 gaaaagtttt ctggattaat gccaatagcc acccgaactt cattcaattg atttttttga
     7981 acaaaagctt caaagttgcc actatacatt agttgaatag caaacacatt cccagcatcc
     8041 tctgtgactc cttgttcgca tagtagaaga gctggtgttt gagcatgacc agaagcacct
     8101 cggtttgaac taatcgaaaa gattccttgt tctacctgtt gacgtctaac agtcttttca
     8161 cgagcataag caccctgcag agttactatt tcgtaatctg cagctggaaa atcagccata
     8221 aaagaaaaat ctttatggat gacaacttcc tgattactat tattatctaa tttactgtag
     8281 ctagcaatag tcgcatcatt attaaaagta gtataataca aagtcagact aagttgagcc
     8341 ttagaatctt ctaacattaa gacaagagtc tctgtatcgt ccatgctatg tggagaaggt
     8401 aagccctgtg gaccattctg accttttaaa atctttgctt ctacaaatcg aaagtctgtt
     8461 acttcagtta cactatgctg aacctgtata gttggtttcc taaaatctcc taagccatgt
     8521 tgtccaaaaa tctgtcgctg agtatctaaa ctaaaggttc gattagtagc cgttggattt
     8581 cctgaaaagg catggtctcg ttcataaaca ctattggaac ctttatagtt cttaatagtc
     8641 tttcctaaat gtttcaaaag taagtagcca tttcgatttt caataatcaa acttagattt
     8701 ttactctcaa cataaaatag attattctct atcctaactc ccatttactt cacctcatca
     8761 ctttattgat tatattttat cacctgaaat cgctttccaa aatagaaaaa tgtctcaaga
     8821 atatggtaaa atgttaggta ggaggtagca catgctagtt ttttcagaat accagactgg
     8881 aacaattgac cttgccctaa gcttttatgg atatgaggaa tgcacaccta attactcttt
     8941 tggtccagcc attcgtgata catacgtcct acattacatt agtaaaggac aaggaaaatt
     9001 tcattacaag ggtaaaattg ttgatttaaa agaaggagat ttctttctat taaaaccaga
     9061 ggaactaacc ttttatcaag cagatagtaa agaaccttgg gcctactact ggttaggaat
     9121 cactggaggg aaatcccctg attattttgc tctttctcaa atttctgatc aatcctatct
     9181 catccaatct gaaacttgtc atacccagac tactgcaaaa ctcatctcag acattgtccg
     9241 cttcgctcag attacaaaat caagtgaatt agctcaactc catatcatgg gacaacttca
     9301 tgaactgatg tttcatctgg gaactattgc tcccaatcag aaaaaaaaga atatttcatc
     9361 aacccaccaa ctctatcttg aatgcaaacg attaattgat agccactatc ctcaatcact
     9421 tacaattcaa gatttagcaa aagaactatc cgttcacaga agctacttat caagcgtatt
     9481 caaagaattt aataccttat cacccaaaga atacctactc tacgttcgaa tgcaccgagc
     9541 tagacaactt ctcgaaaata cccaagagtc catcaaggta attgcatact cggtaggttt
     9601 ttcagatcca ctccattttt cgaaagctta taaacaatac tttaatcagt ctccaagtca
     9661 tacaagaaaa gaatactctc aataccaact agtgagaaag gcaacattat gaaatcctac
     9721 caagctgtct accaaatcct atctaaagaa accgactata tcagcggaga aaaaatcgca
     9781 gaaaaactat ccctaagccg aacatcaatt tggaaagcta tcaagcgact agaacaagaa
     9841 ggcattgaaa ttaatagtat caaaaataga ggatataaac taatgaatgg tgaccttatt
     9901 cttccagaga ttctagaaga aaatcttcca attaaagtca gctttaaacc cgaaacaaaa
     9961 tcaacacaac tagatgcaaa agaagcaatt gatttaggcc atgaagcaaa tactctctat
    10021 ctagcttcct atcaaacagc aggccgaggc cgttttcaac gttccttcta ctcaccacaa
    10081 ggtggaattt atatgacact ccatcttaaa ccaaatctcc cctatgacaa attaccatcc
    10141 tacacactac ttgtagctgg agctgtctac aaagccatta agaacctaac tttaatagat
    10201 gtcgatataa aatgggtcaa tgatatctat ctaaacaatc ataaaattgg aggaatcctt
    10261 actgaagcaa tgacctctgt agaaactggc ttagtcacag atatcattat tggagtaggt
    10321 atcaatttca ctattaaaga cttccctcag gaattaaaag aaaaagctgc cagcttattt
    10381 aaagctacag ctcctataac aaggaatgaa ttgatcatag aaatctggcg tgctttcttc
    10441 gaaacaccag cagaagagct attataccta tacaaaaaac agtcattcat tctaggaaaa
    10501 gaagtcactt tcacactaga gcaaaaagac tacaagggac ttgctaaaga catctcagaa
    10561 aatggaaaac ttttagttca atgtgataac ggaaaagaaa tctggctaaa tagtggcgaa
    10621 atttctctca atagttggaa gtaaaataac acaattataa tataaacgat ataaaaataa
    10681 cttcagatta gtaattcaat taagttttac ggatctgaag ttttattgtc tctaaaaaca
    10741 aaaaagagag ttacaaactc tcattaaaac ggagaataag ggattcgaac ccttgcgcca
    10801 gttacccgac ctaacgattt agcaaaccgt cctcttcagc ctcttgagta attctccaat
    10861 taatgggcac gagtggactc gaaccaccga cctcacgctt atcaggcgtg cgctctaacc
    10921 acctgagcta cgcgcccaag ttaaaaaact tggtaatttg aacaaagttc aaagcgggtg
    10981 acgagaatcg aactcgcgac aacagcttgg aaggctgtag ttttaccact aaactacacc
    11041 cgcataaata ctatcaataa aatggcgcga gacggaatcg aaccgccgac acatggagct
    11101 tcaatccatt gctctaccaa ctgagctacc gagccttatt gcgggagcag gatttgaacc
    11161 tacgaccttc gggttatgag cccgacgagc tacctagctg ctccatcccg cgttaatata
    11221 aaaggaggat gtgggattcg aacccacgca cgcttttaca cgcctgacgg ttttcaagac
    11281 cgttcccttc agccggactt gggtaatcct ccaatattca aatggacctt gtaggacttg
    11341 aacctacgac cactcggtta tgagccgaga gctctaacca gctgagctaa aggtccgaca
    11401 agatcattat agcggcgaag gggatcgaac ccccgacctc ccgggtatga accggacgct
    11461 ctagccagct gagctacacc gccatgaatc gggaagacag gattcgaacc tgcgacacct
    11521 tggtcccaaa ccaagtactc taccaagctg agctacttcc cgagttaaaa agaaaaatgc
    11581 accctagagg agtcgaacct ctaaccgcct gattcgtagt caggtactct atccagttga
    11641 gctaagggtg ctccatatta tgccgaggac cggaatcgaa ccggtacgat cgttaccaat
    11701 cgcaggattt taagtcctgt gcgtctgcca gttccgccac cccggcctct ctaagcgaac
    11761 gacgggattc gaacccgcga cccccacctt ggcaaggtgg tgttctacca ctgaactacg
    11821 ttcgcactgt tttcttctat ctaaaaatgc cggctacatg acttgaacac gcgaccctct
    11881 gattacaaat cagatgctct accaactgag ctaagccggc tcatttgtta tatcttaatg
    11941 cgggttaagg gacttgaacc cccacgccgt taagcgccag atcctaaatc tggtgcgtct
    12001 gccaattccg ccaaacccgc atatatgacc cgtactgggc tcgaaccagt gacccattga
    12061 ttaaaagtca attgctctac caactgagct aacgagtcta aaataacttg cgttatctta
    12121 aacggtcccg acgggaatcg aacccgcgat cttcgccgtg acagggcgac gtgataaccg
    12181 ctacactacg ggacct
//
LOCUS       DAVLXF010000058        12157 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLXF010000058 DAVLXF010000000
VERSION     DAVLXF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.058"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..803
                     /locus_tag="VYO12_001494"
     CDS             <1..803
                     /locus_tag="VYO12_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1588068.1"
                     /translation="LLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCL
                     DIIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAP
                     RSIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVIL
                     AEIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACAR
                     FSPAFKAYLKTKLEQGKHYNVAIIHLAKKLIRTLFYILKKSCHLTNKK"
     gene            complement(1002..2891)
                     /locus_tag="VYO12_001495"
     CDS             complement(1002..2891)
                     /locus_tag="VYO12_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1588069.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            3029..4594
                     /locus_tag="VYO12_001496"
     CDS             3029..4594
                     /locus_tag="VYO12_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1588070.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4594..5358
                     /locus_tag="VYO12_001497"
     CDS             4594..5358
                     /locus_tag="VYO12_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1588071.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQARPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(5557..5772)
                     /locus_tag="VYO12_001498"
     CDS             complement(5557..5772)
                     /locus_tag="VYO12_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588072.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(6643..7893)
                     /gene="ilvA"
                     /locus_tag="VYO12_001499"
     CDS             complement(6643..7893)
                     /gene="ilvA"
                     /locus_tag="VYO12_001499"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1588073.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7943..8299)
                     /locus_tag="VYO12_001500"
     CDS             complement(7943..8299)
                     /locus_tag="VYO12_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1588074.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(8537..8773)
                     /locus_tag="VYO12_001501"
     CDS             complement(8537..8773)
                     /locus_tag="VYO12_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588075.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(8880..9902)
                     /gene="ilvC"
                     /locus_tag="VYO12_001502"
     CDS             complement(8880..9902)
                     /gene="ilvC"
                     /locus_tag="VYO12_001502"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1588076.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVVEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGSRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9968..10444)
                     /gene="ilvN"
                     /locus_tag="VYO12_001503"
     CDS             complement(9968..10444)
                     /gene="ilvN"
                     /locus_tag="VYO12_001503"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1588077.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(10437..12137)
                     /locus_tag="VYO12_001504"
     CDS             complement(10437..12137)
                     /locus_tag="VYO12_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1588078.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         3686 a         2752 c         2120 g         3599 t
ORIGIN      
        1 aacttctttc tgaccccaat cgtgaacaat ttcaacacga taatcggcaa gtagacctaa
       61 aaatactggc tagacatatt catcgtctca agaaaaaaca gtctgattgg aaagtacaat
      121 acactcgttg tcttgatatc atctttcctg agttggataa aatcgttgga aagcattcag
      181 aatataccta ccaactcttg acgcgctacc ctaatcctca gaaaaggatt gaggcaggat
      241 ttgataagct gatagaaatt aagcgattga ccgcttctaa aattcaggat atcctctcag
      301 tcgcacctcg ttctatcgga acaacatctc ctgctcgtga attcgaaatc atcgaaatca
      361 tcaaacatta caagaggctc attgacaagg cggaaacatg tgtcaatgac ttgatggctg
      421 agttcaactc agtcatcacg acggttactg ggattggggg tcgtttaggg gcggtcattt
      481 tagccgagat tcgaaatatt catgcctttg ataatcctgc tcaattacaa gctttcgctg
      541 gactggattc ttctatttat cagtcaggtc agattgattt agctggaaga atgatcaaac
      601 ggggttcccc tcatctgcgg tgggcactca tacaagctgc caaagcatgc gctcgctttt
      661 cacctgcttt taaggcctat cttaagacta agttagaaca aggaaaacat tacaatgtag
      721 ccatcatcca ccttgcaaaa aaacttatcc gaaccctgtt ttatatccta aaaaagagct
      781 gccatttgac gaacaaaaag tgatgtaatt tctgaaatta acacaggatt acctttgaaa
      841 aaatggactt tttcaagggc tttttgccgt gctctaaaat catcgccaaa tcatcaaatt
      901 gaaataagaa atagtttaga taaaactatt gacttttctt atagtttgtc tttcttctat
      961 cttccattat atcatacttt ttcagtctat ggtagctctc tttaaaaggc tccgatactg
     1021 ccgccacctc cgcctccaga gaagccaccg ccagaacttc cacttccaga agatacggag
     1081 taggtacttg ctgtatttgc gacactagca taatggctca tttgcgcgct tgaatgataa
     1141 aacatactgt gccagccata agctacatag agattgatat ctggattttc aacttgaatc
     1201 tgatgaacct tcatcaaatg actaaccttg tccgcatagc caaataaggt tgcatagacc
     1261 aagagacgat tccaaacaac aatactttcc aattcagcct tatccagtcg tgcaatctca
     1321 cgtaacatat tttcaaaact ggtccagaga tagtagacct cagctcccgc ttcatttaga
     1381 acaccatcac gattatctag tcgaagcttc caataataga aaacagacaa aactaaccct
     1441 agaaaaccaa gtattggcaa agggaggtaa agatagccat gaacgtctaa actgtacaag
     1501 aacaaaccaa atccgataaa taggggcaag atagtcaaga cacccatacc cacttgcaaa
     1561 gtcttttcca aaccagttaa aggacgatag taatctggga gcccccagaa ggaaactcgt
     1621 tttctcactc cttcttgcat ctggttcaat acctcttcaa aagaagattt gagttgaagc
     1681 cctcttgctt gaatccgttt ttcatcagaa actttggctc tacgataaag actatcagat
     1741 accttgtaat ccgcaaacaa attggaaaga gtggcttctt ttttgcctga aaaggctaga
     1801 tttagacagt ctctctcaaa tcttgacaaa ccatcttctt tcaccaatct caagccaact
     1861 gcatctcctt ctgaaataat agagacattc ccacggtcta tcacatctag caaggtagct
     1921 tgaataagtt gatcaaaggt gaattttcca gctcccttga ccaagggact cacttcctcc
     1981 aaggaggtcg agtagactgc ttctgataaa accataggct ctaattccat tggtggttca
     2041 tagagacgat gatttttggc atatttgact gaaggagtgg tctttcttct ataaataaaa
     2101 tagaagcaga cactcaataa caaggagatg gaaaggatcg aagggaggac ccaagtaacg
     2161 agttgtttac tctgatcttt ttctctaaca atcgagtctt ctatcttatt aaactcttct
     2221 aaacgattcc ctttcaagcc ctgatcccta gcgctagcaa aatcggtccg agaccaatag
     2281 gcatgcaact caactccacg cttagccgga agattgtcta aacggatagt ataatcaagg
     2341 ttactctttt caatcgttcc ctctctaaaa agtttccctg tatggaaaaa gagtttttca
     2401 gcccccttgt ctccccttac atgaaattca aacttttcaa tagactctga actatctgtc
     2461 agaggttgcc aatttaattc agcgatatca tcataaagga aaagtaaatt ttttaagttc
     2521 cagacgaggt caacttcaac tatgtcgccc tcctgacctg gattatagac tctcacagta
     2581 taaccatccg cttcttctgt tacttcgcta gtcacatctg ctagttctgc accgtttttc
     2641 gcagcctgaa cctttggatg agggtcaatg tcaaacccgc taggcatctt accagcacgt
     2701 ccaagtccca cgatttggcc cttaaagtcc tcctcaaact ggtaaactat cttctgtcta
     2761 aactctgccg tattatctgc atgaatatac aaatcccctt gataagagtt tatcttgaaa
     2821 tcaatggcaa aaacagagag tggaagaagg caaaacaagc ctaacaccag taagaaaaaa
     2881 gtttttttca tcaactaagc ccccttttta tctttcttat cattatagca ttttttcgta
     2941 agtaaaggct tacttatgtt gaaaaattac gcatttttcg ataaaatagg agacagttat
     3001 tttttaatag atcagaatag gagaaatcat gagaaaaata tacttatcta ttttcacaag
     3061 tctcttgctg atgctaggac ttgtcaatgt tgctcaagcc gatgaatatt tacgcatcgg
     3121 gatggaagca gcatatgctc cctttaactg gacccaggat gatgatagca acggagctgt
     3181 caaaatcgat gggaccaatc agtatgccaa cggatacgat gttcaaatcg ccaagaaaat
     3241 cgctaaggac ttaggtaaag aacctttggt tgttaaaacc aagtgggaag gtctagtccc
     3301 tgcccttact tctggtaaga ttgacatgat tatcgcaggt atgagtccaa ctgcagaacg
     3361 caaacaagaa attgcctttt cgagcagtta ctacactagc gaaccagttt tgcttgtcaa
     3421 aaaagattct gcctacgcaa gtgctaaatc tttggatgac tttaacggtg caaaaatcac
     3481 ttctcaacaa ggggtctacc tctacgactt gattgcacaa atcccaggtg ctaaaaaaga
     3541 aacagccatg ggagacttcg ctcaaatgcg acaagctctt gaggctggtg tcattgatgc
     3601 ttatgtttct gaacgtccag aagcactgac tgctgaagct gcgaactcta agttcaagat
     3661 gattcaagta gaacctggtt tcaaaactgg ggaagaagat acagctatcg ctatcgggct
     3721 tcgtaaaaat gacaatcgta ttagccaaat caatgccagc attgaaacca tttcaaagga
     3781 cgaccaagtt gccctgatgg atcgtatgat caaggaacaa cctgccgaag ctacaacaac
     3841 tgaagagact agcagtagtt tctttagcca agtcgctaaa attctttctg aaaactggca
     3901 acaactcttg cgtggtgctg gtatcactct tttaatctct atcgtcggaa ccatcatagg
     3961 tctcattatt ggacttgcca ttggtgtctt ccgtactgct cctctctctg aaaacaaagc
     4021 catttatggc cttcaaaaac tagtcggctg ggttctcaat gtctacattg aaattttccg
     4081 tggtacgcca atgattgttc aatcgatggt tatctactat ggaactgccc aagctttcgg
     4141 gatcaacctt gaccgtacac tggctgctat cttcatcgtt tcaatcaata ccggtgccta
     4201 catgactgaa atcgtccgtg gtggtatcct agcagttgac aagggacaat ttgaagctgc
     4261 gactgctctt ggtatgaccc ataaccagac catgcgtaag attgtcctac ctcaggtagt
     4321 ccgtaacatc ctacctgcaa ctggtaatga atttgtcatc aatatcaaag atacatctgt
     4381 attgaacgtt atctctgttg tcgaacttta tttctcagga aataccgtgg caacacaaac
     4441 ctatcaatac ttccagacat ttacaatcat cgccgtgatt tactttgtcc tcaccttcac
     4501 cgtaacacgt atcctacgct ttatcgagcg cagaatggac atggatacct acactacagg
     4561 tgctaaccaa atgcaaacgg aggatttgaa ataatgacac aagcaatcct tgaaattaaa
     4621 cacctcaaaa aatcctatgg acaaaacgaa gtgctaaaag acatttctct cactgtccac
     4681 aagggagaag ttatttctat catcggaagc tctggaagtg ggaaatcaac cttcctacgc
     4741 tccattaacc tacttgaaac gccaactgat ggacaaatcc tttatcatgg acaaaacgtc
     4801 ctcgaaaaag gctatgacct cacgcaatac cgtgaaaagt tagggatggt tttccaatcc
     4861 tttaacctct ttgaaaatct caacgttctt gaaaacacaa tcgtcgctca gacaactgtc
     4921 cttaaacgcg aacgcacaga agctgaagaa attgccaaag aaaacctgga aaaggtcggc
     4981 atgggagaac gctactggca agcgagaccc aaacaactct caggtggtca aaaacaacgt
     5041 gtggccatcg ctcgtgccct ttcaatgaat cctgacgcca ttctctttga tgaaccaaca
     5101 tcagctctcg atccagaaat ggttggagag gtcctcaaaa tcatgcaaga tctggctcag
     5161 gaaggcttga ccatgattgt cgtaacccat gaaatggaat ttgcccgtga tgtctctcac
     5221 cgtgttatct ttatggataa gggtgtgatt gctgaagaag gtaaaccaga agacctcttc
     5281 actaatccta aagaagaccg tacaaaagaa tttcttcaac gttatctcat actcaatgaa
     5341 aatcaaagag caaactaggc agctagccgc aggttgctca aaacactgtt ttgaggttgc
     5401 agatagagct gacgtggttt gaagagattt tcgaagagta tcaaataaaa agaaaaggct
     5461 gcatcaaccg tgcagtcttt ttgctgtcct caaaatgaaa aggcagattc tctatctcta
     5521 ctacttgaag gtcaagaatc caccgttatc caaagattaa tcttcaaatt tttcatgatt
     5581 tttttcatag aaatcaatta agcctagggc tgtttcaaat gaaatatttt ttacttttgc
     5641 acgaccctgc gccagagcaa tgatagacat ttcacgcgca ttcgtttctt tagagatacg
     5701 gtagcctgtg attttcttat cacgaaccca gccaacaact gattctactt tttcaaagtt
     5761 tgacttagcc atgtttttct cctattttct tctttataat atttaattgt atacgttttt
     5821 actacttttg tcaataaaaa aaacatatat tcaacgaaaa taattagttc agaattttct
     5881 tacttccttt ttaaatccga taatatatag gttttgctag cctctacacc ataaatattt
     5941 gctaataaaa atactgtatt attaccctct taaggtgcga aattattcaa gttcttaaaa
     6001 aatgcttctc tatttccgta agatagctta tttcttcgat ttaagaactg aagatgatag
     6061 ttctaacctt tagaaatcca agccttctct agctcaaaaa ctcctatctt tcttcctcaa
     6121 taaatcatct gagagatagg tattccttat atccttcaaa ttcatcactc ctttggatat
     6181 tgaggtgcta gcctagaagc agacacccga ttatctaaac catccttaat agagatgaca
     6241 agacagataa gctcagttaa ttacttatat atctttttaa tatggtatat ttgtcaacaa
     6301 aaagaaatca aatcatacct tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
     6361 cgcaaattgc tcaaaacact gttttgaggt ttcagataga gctaacgtgg tttgaagaga
     6421 ttttcgaaga gtattaaaga cagtatgact atgcttgtca atagatagga aatcaaaatg
     6481 aaggatggaa atctgagcca aacatcataa gtgctataat gaacagaagt aagaattgca
     6541 cgataagttt tctaaaacca taggaatcgt cccctttctt catcttcatt atagcacata
     6601 tacatcagtt gtacaaataa tatgatattt ttttattagt cctcagacaa gcatattata
     6661 aagcgtttca ttaccattta agttaatata agctggatca aaaccttcca ttctacggat
     6721 caaacctgca taatcatgct tatctgccaa agcgatccca atcaatactg ggcctgttcc
     6781 cttgctagct cgtttgatat actcaaaacg tgtgatatca tcatttggcc ccaggatatc
     6841 atttacaaac tcacgcaaag ctcctggacg ctgtgggaaa ttgaccacaa agtaatgttt
     6901 gataccatca taaatcaagg cacgctcttc catttctggc atacggttga tatcattatt
     6961 tcctccagaa atgatacaac aaatggtttt ccccttaata tattcagcta aaacctctaa
     7021 agaggcgata ctagccgctc cagcaggttc tgcgactatc ccttgcttag agtaaaggtc
     7081 aatcaaggtt tcagaaatca atccctcatc gacacctacc aaagtttgaa tatgttgacg
     7141 agttgcttca taggtcaact gacctacctt ttgcacagca atcccatccg caaatttatc
     7201 aatttccttg agtttgactg gacctccagc ctcaaaggca gctttcatgg aacgcgctcc
     7261 attcgcctct actccgataa cctcaatctc tggacttgtt tccttgatat aggtagaaac
     7321 cccggcaatg agaccgccac caccaacagg aaccaagaca gcatcaaaat caatcgattc
     7381 ttttcgagct tcttctaaaa tctcataagc aactgttcct tgacctgctt gaacatgagc
     7441 atcatcaaaa ggatcaataa aggtacgatt ttcagagact gtaaattctt gagctgcttt
     7501 ggctgaggca tcaaaggtat ctccaactag tttaatagtt acaaaatccc caccaaaaaa
     7561 gcgaacctga ccaatctttt gttgtggcgt agtaataggc ataaagatag tagcaggaat
     7621 tttcatttca ttacaagtat aggctactcc ctgcgcatga tttcccgcag aagcgcagac
     7681 taccccacgt tcacgttctt ccttgctgag ctgggaaatg gcataatagg caccacgaat
     7741 tttaaaggag cgaacacgct gggcattttc ttttttcaaa taaatcttag caccatactt
     7801 ctccgataaa taatgatcgt aatccagtgg agtattcaca accacaccgt tcaagacctt
     7861 gtgagccttg attatatctt ttgaacttaa catataaaac ctctttttct ataaaaaaca
     7921 gtcactaatg actgtttttt agttattcat ctccgaaaac ttcagtaaca ctttcatggt
     7981 atttatgagc catacgaatc gaaaactctt taggaagttc ataacgattt gtggaaatcc
     8041 aatcatataa ccattctacc aaactttctt cactatcatt acgatggaag ataaaatagt
     8101 ttaaaatttc tttatattct acatcattca aatccaacgt gtctacaaat tgactacgag
     8161 gaacaaaatt agaatctaat tttcttagtt gatctgtcaa ttctcccaat tgtaaatcca
     8221 actgaaaaaa tagtttttgc agttgcctat cagtttcatc acatcctgtc ttgatagaaa
     8281 acaaattggt aatattcata tgatttcaac ccctaaataa gtacatctaa tatcccacga
     8341 atagctgaag ctaaagtttt cgcagtccag tcatttaaac ccgattcttt caatccagat
     8401 gcaataattt gctctatttg atataaaccg ccatctatcg tctcatttat tctgcgaaga
     8461 ggaccttcaa tataggataa agcttctccg aatctaacag ctgatttagg tcctaatgat
     8521 ttaccaacta tatttgttaa tgttttttat ttctcaaaat ccatttcgct atataacgag
     8581 cagatttagt cgcaccagct ctctcaaacg gagaatgaat ttcaagacct tcttctctag
     8641 ctatttgtgc caatttgaag aattgttcaa aggtcatatg caacctacca tcttcaaaat
     8701 tagcatcatc ataaatgtta attacagcat tttcatcata cgaccaatct gctttatttt
     8761 ggtctgctag cactattacc gacggtgtaa ttacattata caactagaac caataataac
     8821 gatgcaattt ttctcataat ctttttcata aaatcatctc cagcgctata tatttctaat
     8881 tagttataga ttttgaatgc atcatcgtcg tttttaccaa caaatggcat tgctttacgc
     8941 aattctgcac caactttttc aatttcaagg ttagctgctt gttcacggta agcagtcaat
     9001 tttggacgtc cagctttata gtcatttaca aagtcatttg caaatttacc attttggatg
     9061 tctgccaaga cagccttcat attttcttta acttgttcag tgattacacg tgaacctgat
     9121 acatagtcac cgtattcagc agtgtttgaa atagattgac gcattttctt gaatccacct
     9181 tcgtagatca agtcaacgat caatttcatt tcgtgaagaa cttcaaagta agccaattct
     9241 ggagcgtaac ctgcttctgt caagacttcg aaacctgctt cgataagggc agtcaaacca
     9301 ccacaaagta cagcttgttc accaaacaaa tcttcttcag tttcttcttt gtaagttgtt
     9361 tcaagaagac ctacacgagc cgctccaaca cctttacacc agtccatagc aatgttttta
     9421 gcatttcctg ttgcatcttg gtatactgca taaagagctg gaacaccaaa tccttcttcg
     9481 taagtacgac gtaccaagtg tcccggtcct ttaggagcac acatgaagac atctacatcc
     9541 gcaggaactt tgataaattc aaagtggatg ttgaaaccat gggcaaatcc aactgcgttt
     9601 ccagcttcca agtttggagc gatttctgct tcgtacaatt cttgttgaat ttcgtctggc
     9661 gccaagatca tgataacatc agccaactta gtagcttcta ctactgtgta agtatcaaat
     9721 ccatcttctt ttgctttatc aaaagattta cctggacgta caccgataat aacgtcacga
     9781 cctgaatcac gcaagttttg agcatgcgca tgtccttgtg aaccataacc gataacggcg
     9841 atttttttac cgtcaagtgc tgctacttta acatcttttt catattccat ttgaactgtc
     9901 atagcttttc tctcttttct attatttatt gccttttagg ctggtttaat aaatttaagt
     9961 tggattttta atcgcgggta aatccagttg cacccgttcg agcaatattg cgaataccgt
    10021 atgggcgaat gactcgcaat agggcttcgc tcttttctgc atttcccgtc atctgaatgg
    10081 taatcgagct tggcgctacg tctaccactg ttgcacggaa aggttgaata atcgctaaaa
    10141 tctcagctct cttctcagct ggcgctgaca tcttaaccaa aatcacctcg cgctccaaat
    10201 gaggcttgtc tgtaatatct cgaatgcgaa tcacatcaat ctgacgattg agctgtttga
    10261 tgatttgctc cacttcatca tgagaagcaa catcaataat aatagtgata cgcgatacat
    10321 tcggatcttc tgttgctcca acagagatgc tttcaatatt aacctgacga cgagatagga
    10381 cacctgtaaa gcgattgagg actcctgaac gattttgtag ttttgctgtt aacattctac
    10441 gcatggaact gcacccccaa catctcatga ttactcttac cagccggtac cattggtaac
    10501 acctgttcct tacgagaaat atctacctca attagcatag gaacatcctc agtgatgact
    10561 tcaaggtctt gagccaaggt ctcaggattg tcaaacttat agtttttaat accataagcc
    10621 tgcgccatca attggaaatc aggaagggta tcaaagaccg actctgatgt tctgccttca
    10681 tagaaggatt cctgccactg gcgaaccatt ccaagtgaat gattgttcag cataaccacc
    10741 ttgattggca ccttgtaaat attcaaaata gccaactcct ggttggtcat ttggaaacca
    10801 ccatccccaa caaacaagac tacttcctta tctgggttag caattttagc accgattgct
    10861 gctggaattc caaagcccat tgttcccaaa ccacctgaag tcactaactg acgctcattt
    10921 tggtagggat aatactgagc tgtccacatt tggtgttgac caacgtctgt taccacaatg
    10981 gcatctccat tcgtcaattc accaattcgt tcaataactg cttgcggttg aaccacacgc
    11041 tctttcttgt cataagaacg aacacgattc ttgtctttag tgactttctc aatccacttt
    11101 tcagtattgt tgtgaactgt tggttctgct agcaacattt gcaaggcctt cttagcatct
    11161 ccaactacag gaatgtctgc actgataatc ttgccaatct cagctgggtc aatatcaatg
    11221 tgggcaacct tagcattctt agcgaaagtc ttaggattcc ccgtcaaacg gtcatcgaaa
    11281 cgagaaccaa tactaatcat aaagtccgct tccgtcatgg caatatttgc tgcgaatgac
    11341 ccgtgcatgc ctcccattcc aagaaagagt gggtgactcg ttgcaatcgt tccttgtccc
    11401 aaaagactgg ttaccactgg aatttgatag cgttctgcaa attcatttag ttccgtagca
    11461 gcctcagcat aactaattcc accaccagct aacaagactg gctttttagc cttggacaat
    11521 tgcttcaaga ttttcttgat ttgcatatca ttcggctcaa gagtcggctg ataacttggt
    11581 aaattcactt ctggtgaata aatgaagtct gtttctaaag cagatatgtc ttttggtagg
    11641 tcaattacaa ctggccctgg acggcctgta gttgcgatat ggacagcttc cgtaatgata
    11701 cgcggaatat cagctgtctc acgaacttgg taattgtact tagtgattgg catggtaatt
    11761 cctacgatgt ctgcctcctg aaaggcatcc ttcccaatcc ctgctcgcgc cacctgacct
    11821 gtaaagacca aaaggggaac gctatcgctc atagcatccg caatccctgt aatggcattt
    11881 gttgctcctg gtccactagt gacgacggca acacccaact ttccagttga tttggcataa
    11941 ccttcagctt catgcaaaca accttgctca tggcgcccta gaatgtggcg aatgccttta
    12001 aaattatata tcgcatcata aaaaggcaaa accgcaccac caggataacc aaagatggta
    12061 tcaactccta aatcacgaag tgtttccaaa actaggtccg accccgtctt aggagattct
    12121 aaactgattt tctccattgt tcccctttct ttcctct
//
LOCUS       DAVLXF010000059        12075 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLXF010000059 DAVLXF010000000
VERSION     DAVLXF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.059"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..1151)
                     /locus_tag="VYO12_001505"
     CDS             complement(<1..1151)
                     /locus_tag="VYO12_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1588079.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKEDIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVINNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTIIYLDKISQINQN"
     gene            1209..2375
                     /locus_tag="VYO12_001506"
     CDS             1209..2375
                     /locus_tag="VYO12_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1588080.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            2514..2840
                     /locus_tag="VYO12_001507"
     CDS             2514..2840
                     /locus_tag="VYO12_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1588081.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            2827..3420
                     /locus_tag="VYO12_001508"
     CDS             2827..3420
                     /locus_tag="VYO12_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1588082.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYATRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            3413..4324
                     /locus_tag="VYO12_001509"
     CDS             3413..4324
                     /locus_tag="VYO12_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1588083.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            4387..5451
                     /locus_tag="VYO12_001510"
     CDS             4387..5451
                     /locus_tag="VYO12_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588084.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIANLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLKSPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(5523..6383)
                     /locus_tag="VYO12_001511"
     CDS             complement(5523..6383)
                     /locus_tag="VYO12_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1588085.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(6505..7491)
                     /locus_tag="VYO12_001512"
     CDS             complement(6505..7491)
                     /locus_tag="VYO12_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1588086.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(7763..9238)
                     /locus_tag="VYO12_001513"
     CDS             complement(7763..9238)
                     /locus_tag="VYO12_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1588087.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(9322..10245)
                     /locus_tag="VYO12_001514"
     CDS             complement(9322..10245)
                     /locus_tag="VYO12_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1588088.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(10259..11188)
                     /locus_tag="VYO12_001515"
     CDS             complement(10259..11188)
                     /locus_tag="VYO12_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1588089.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(11507..11857)
                     /locus_tag="VYO12_001516"
     CDS             complement(11507..11857)
                     /locus_tag="VYO12_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588090.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
BASE COUNT         3598 a         2584 c         2214 g         3679 t
ORIGIN      
        1 atattttgat ttatttggct tattttatcc aaataaatta ttgttccctt gtactccttc
       61 ttccatttaa atcctctaat taaacctaca atcgtcccaa ggccataagc aaagtgaatg
      121 gaaaataaaa gaaagggcat cacaattaga aatccatttt tatgttttaa taaagtcagc
      181 aaagtgagta atgacaaaag tagaaaatag gcacctaata gtaaagttat gaatacgaat
      241 gtgatcggta acaatgctag actaaacaca agactcaaaa caaataaaca aggaacatag
      301 tgaaataatg ataaacactt aaactgaaca tgacttgtca agccaatcca caaaccattt
      361 gaatactttt gatgcagcat tttcttgaat gttggtcgaa tatactgata agatagaata
      421 cttgggctat agcggatttt ataaccatgt tctcgaattc tataatgaat atcattatct
      481 tcagttcggc caagttgctc atttactaaa ccaaccttct ggaaaacctc tcgtttatac
      541 atcccatgaa aaatagaaga aacatatcta tcctcggaac tatttcgata attggcaatg
      601 ctactgccaa acatattttc ctcaacaaga tgcaaggtct ctgcccattt tccttttcct
      661 tcgacaatcg tcggtctagg ccccccacag acaaattcac cttgttgaat aatagccaca
      721 ttgttaatta caaaactctc agtaactttt gaatgagcat caatttttaa aataaggtcc
      781 cctacagaat gtttaactcc caggttaaaa ccactagctt gatttttctt aggattgtta
      841 tacaatctaa ttgagttaaa ctctgtatct tcctttataa attgctgaat gatagctgtg
      901 gtcccatctg tggacatagc atttataaat agaatttcaa tatcctcttt aggataggtt
      961 tgatttttta agtcttcaat tagaccaggc agatattttt cttcattgta tgcactgatt
     1021 acaatggata cgagaagact ttcaacattt tgagtcacta ctactctcct gaatttttcc
     1081 ttccaccatt ctatcataat tttcaagact ttcccatttt tatcttgaaa gccagaaacc
     1141 ttacttgaca ttatagtgaa aaagccttaa aataagctgt tattaaaatc agctagaaga
     1201 ggtattatat gaaaaagcaa tcactctttt ttgttccagg tattatcctg attggtgttt
     1261 ccttgcgaac tccttttact gttttaccca ttattttggg aaatatttcg caaggtctgg
     1321 aggtagaagt tagttcgctt ggtgtcttga ccagcctgcc tctccttatg tttaccctct
     1381 tttcaccatt ttctacccaa ctggctcaga aaatcggctt ggagcatctc ttcacctaca
     1441 gcctcttctt cttgaccatc ggctcactta ttcgactaat caatctgccc ctgctctatc
     1501 taggaacctt gatggttggg gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta
     1561 tccaagccaa tcaaccaaag aaaattggtt ttctgaccac cttatatgta acgtctatgg
     1621 ggattgcaac ggctctggct tcctatctag ctgtgcccat tacacaagcc agttcttgga
     1681 aaggacttat ccttctcctc acgttactct gtctagcaac ttttttggtc tggctcccaa
     1741 atcaccgcta taatcataga ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca
     1801 tgcgtaataa acaggtttgg gcaattatta tcttttcagg ttttcaatcc ttgatctttt
     1861 acaccgtcat gacctggtta cctaccatgt ctatccatgc aggtctatcc agtcacgaag
     1921 ctggcttgct gacttctatc ttatctctga ttagcattcc tttttcaatg accatcccaa
     1981 gcctgacaac cagtttatct actcgcaacc gtcagctcat gctcactctg gtttcactag
     2041 ctggtgtggt cggcatttcc atgctctttt tcccaatcaa taatttcatt tactggcttg
     2101 ccatccatct cctcatcgga accgcaacca gtgccctctt cccttatctc atggtcaact
     2161 tttcactcaa gacaagcgcc cctgaaaaga cagcccaatt gtccggccta tctcaaacag
     2221 gaggctatat cctagcagcc tttgggccaa ccctctttgg ttacagtttt gacctgttcc
     2281 actcttgggt accatctgta gctgccctct tgctcatcga tatcctgatg actgtggccc
     2341 tctttacagt ggacagagcg gataagatcc tttaaacttc tagttttagc tagaagtttt
     2401 ttatatataa aaattttaca catttctctt gacagggctt tcttttagat gtacaatgta
     2461 tgtgtagaaa aattatatat aaaaatctta cacattagaa aaggaggttt cccatgtact
     2521 ttccaacatc ctctgccttg attgaatttc tcatcttggc tgtactggag cagggtgatt
     2581 cttatggtta tgagattagc caaaccatta agctgatcgc taatatcaaa gaatccacac
     2641 tctatcccat tctcaaaaaa ttggaaggca atagctttct gacaacctat tctagagagt
     2701 tccaaggtcg catgcgcaaa tactactcct tgacaaacgg tggtatagag cagctcttga
     2761 ccctaaaaga tgaatgggca ctctatacag acaccatcaa tggcatcata gaagggagta
     2821 tccgccatga caagaactga atacctgact cagctagaac tctatctcaa gaaactacct
     2881 gaagctgacc gtatcgaagc catggactat ttcagagagc tctttgacga tgctggagtc
     2941 gaaggagaag aagaactcat cgctagtttg ggaactccca aagaagcggc ccacgaagtt
     3001 ctatccaatc ttctcgataa aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa
     3061 attttacata tcgccttgtt agccctcctt gcagcaccta tcggcattcc tctgggaatc
     3121 gccatcctcg tgaccctgtt cgcaattctt gtagccgctt tgactgtcat tctggctttc
     3181 tttgcagttt ccatactggg tatcatcggc ggattcctat ttttagttga aagtttcact
     3241 gtcctcgccc aagccaaatc agcctttatc ttgatttttg gttctggttt actggctatc
     3301 ggtgcttctt cgctagtttt acttggcatt tcctatgcaa ctcgcttctt cggtctactc
     3361 attgttcgtc tggtacaatt tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa
     3421 atggacaaaa ggatttctca tctttggtgt ggtgactacc gttatcggct ttatcctgct
     3481 ttttgtaggt atccaatctg acgggattaa gagcctactt tccatgtcca aagaacctgt
     3541 ctatgatagc cgtacggaaa agctaacctt tggcaaggaa gtcgaaaacc tagaaattac
     3601 tctccaccaa cacacgctca ccatcacaga ctctttcgat gatcaaatcc acatttctta
     3661 ccatccatct ctttctgctc accatgatct tatcaccaat cagaacgata gaactctgag
     3721 tctcactgat aagaaactgt ctgaaactcc gtttctctct tctggaattg gtgggattct
     3781 tcatatcgca agtagctact ctagtcgttt tgaagaagtt attctccgac taccaaaagg
     3841 gagaactcta aaagggatca acatctcagc caatcgcgga caaaccacca tcataaatgc
     3901 tagccttgaa aatgcgaccc tcaatacaaa cagctatatc ctccgaattg aaggaagtcg
     3961 tatcaaaaac agtaaactca caacgcccaa tatcgttaat atctttgata cagttcttac
     4021 agatagtcag ctagagtcaa cagagaatca cttccacgct gaaaatatcc aagtccatgg
     4081 caaggttgaa ctgactgcca aagattatct cagaatcatc ctagaccaga aagaaagcca
     4141 acgaattaac tgggacatct caagcaacta tggttctatc ttccaattca caagagaaaa
     4201 gcctgaatca agaggtacgg aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa
     4261 ggatcaactc attgcgagat ctgatgataa tattgatcta atatccacac caagcagacg
     4321 ttgacaaaaa cgcttacatt tgctactatg gaaacatatt tatctaacta aaaaggaggt
     4381 tctaccatga aacaagaatg gtttgaaagt aatgattttg taaaaacaac aagcaagaac
     4441 aagcctgaag agcaagctca agaggttgca gacaaggctg aagaaacgat agccaatctc
     4501 gatacaccaa ttggaaaaaa tactcaagta gaggaggaag tctctcaagc tgaagtcgaa
     4561 ctggaaagcc agcaagaaga gaaaattgaa actcctgaag gcggtgaagc gagaacagaa
     4621 atagaagaaa agaaggcatc taattctact gaagaagagc cagacctttc taaagaaaca
     4681 gaaaaagtca ctatagctga agagagccaa gaagctcttc ctcagcaaaa agcaaccacg
     4741 aaagagccac ttcttatcag taaatcttta aaaagtcctt atatccccga ccaagctcca
     4801 aaatctaggg ataaatggaa agagcaagtg cttgattttt ggtcttggct agtagaagcg
     4861 atcaaatctc ctacaagtaa gttggaaaca agtatcacac acagttacac agcctttctc
     4921 ttgctcattc tgttttctgc atcttccttt ttctttagta tctatcacat caaacatgct
     4981 tactatggac atatagcaag cattaacagt cgcttccctg agcagctagc tcctttaact
     5041 cttttttcta tcatctctat cctagtagcg acaacactct tcttcttttc attcctcttg
     5101 ggtagtttcg ttgtgagacg atttatccac caggaaaagg actggacgct agacaaggtt
     5161 ctccaacaat atagtcaact cttggcaatt ccaatcttcc tcactgctat tgctagtttc
     5221 tttgctttct ttgatagcct acgatttaca gctctcttgt gtgtgattag cattggaatc
     5281 attctgcttg ccagtctcca tatcattaca agaccaagtc aagcaagtga aaccgactcc
     5341 ttctatcaat tattcttgtc tgtccttgtg aacggagtta ttatcctcct cttctttgta
     5401 gctgaagtgg cactgattgg agattatctt cgtatcttgg cctttcttta aaattcaatc
     5461 ctgtcatcct tggcaggatt ttttctatgt caaaaaagca agtcgattga cctgcttctg
     5521 ctttactttt cttgtgctaa tgttttaaaa tctttgtcta atatgggttg cacttctatt
     5581 tgattggcgt ctagttggtg gtaaaaagat gggggtaata cctctaagaa ttttgcatag
     5641 tccaagcgag ctatggattc atagtcttct ggtttagagc cgtctcctgt gatttgaacc
     5701 aagtcaatct cccactggac ttccttatcc accaattgct caatataggc tgcaggaaca
     5761 aacggttctc tcggtaaaac tgtcttgact ccttgagcta aatggtaaac gccatgcact
     5821 tttccttctc catcctgata gaaggaaact cttgcaaagt aggcatctgt ctcttgaacc
     5881 gcacgcacac gcgcttcaaa ctgagccaag ccatctgcct ctaaaaagct tttcaagttc
     5941 tcacgactaa agaaaatagg attaaaaatt ccttggcaaa tcgtaaaacg agctacaggg
     6001 tctgctagat aggcttgaat acttgcttgg aaataagctt caaaatcaaa accatctagc
     6061 ccttcaatat cagtccctgt ataagcaagc aaagcatcta aaaatgactt gataatctgc
     6121 cagtctgtct ttgttagtag gtcaggaaga tcgacacggt aagccttttg accatcagca
     6181 taactgacct taaagagaaa ttgtgattgc cccactatcg cacactcaat atactcgaga
     6241 cgattaagag gctgacgaag atagactaca tcatagctat gtgactccaa accgtctagc
     6301 aaggccaaga tcgacttggc attcaaaatc tcttgttctc ctaaaatgct ctgtttattt
     6361 ggaataaaaa atgtttttgc cataactgga ctcctttgaa atcgtttaca tacagtatac
     6421 cttattttcc tgaaagatac aaaaacaaac tttagatttt tgagtacaaa gcatagaaaa
     6481 acagcccctg aggactgttt gatcttatac agtagctgcc tgatccaagc tttcaccgat
     6541 agcggctagg cgctcaatca cttcagcttg tgtcaattca ttttttgaaa catagcggtt
     6601 acgtgggtga acacggcact cgtgtgagca tccacgaagg tacttgtctt cattttcttc
     6661 tgatgtcaag atacgacggt tacagaaggg atttccacag ttgacataac gttcacatgg
     6721 tgttccatca aaccagtctt tccctacgat ggttgggttg acatggttga catcaacggc
     6781 aatacgctcg tcaaagacgt acattttccc atcccaaagc tcaccttgaa cttctgggtc
     6841 tttaccgtaa gttgcgattc ctccgtgcaa ttggccgaca tctttgtagc cttcacggac
     6901 catccagcct gagaatttct cacagcgaac gccacctgta cagtaaacca cgacacgctt
     6961 gtccatgaat ttttccttgt tatcacggac ccattgtggt aactcacgga agttgcgaat
     7021 atctgggcga atagccccac ggaaatgtcc taggtcgtac tcataatcgt tacgtgtgtc
     7081 aaggacaacg gtatctttat caagaagcgc ttctttgaac tcttttggag acaagtaagc
     7141 acctgttgtt tcaagtgggt tgatgtcatt gtcaaagtcg ttgtcttcca aaccaaggtg
     7201 gacaatttct ttcttgtagc gaacaaacat cttcttgaag gcttgttcat tttcttcgtc
     7261 aatcttgaac cagagttctt ccattcctgg aaggctgtga acgtagtcca tgtatttttg
     7321 agttgtttca tagtcacctg aaactgttcc gttaattccc tcgtcagcga ctaggatacg
     7381 gcctttaagg ccgattgatt tacagaaagc caagtggtct gcagcaaatt gctctgcatt
     7441 ttcaattgga gtataaaggt agtaaagtaa gacacgaata tcttttgcca taagatttgt
     7501 tctcttttct attcttaaat tttctaaatt ttttatttat ctacactagt atacctgctt
     7561 gagaaaacga atgcaattta tttgactgga aattgtagag ggttctcgaa atttcctgaa
     7621 tggttaaaat aaggacaaga gaaaacatgg atatctatat ccttgtgcca aaaaaaccac
     7681 tgccctcccc agaccaacct gaggaaagcg gtgattctta ttttaggagt taggaatgaa
     7741 tacacgaaat caatttagct gattattttt tgtttttcaa gaattcatcg tattgttttt
     7801 gcatttcgtt caatactttt tcgtaggcac cttcagattt caatttttcc atcaattctg
     7861 gaatcgcttt atctgggtct acagtaccag tgttgatagc tgtatcaaat tgttgcattg
     7921 tgttagcaat agctgagatt tcagatttca cattgtcagt attgaagata aatccaagcg
     7981 ctggagattc tttagcttct gccaattctt tcttagaatt ttcgatttgt tggtctgtaa
     8041 cgttttcgtt gatgtaaagg atccagttgt taccagtgtt ccatccaccc atgtgagtgt
     8101 ttcctttgta gccatcaaga acgcgaacac ggttttcttt accttcaatt ttttcccagt
     8161 tcttgccttc tggaccgtaa acaagaccgt tcaaaagttc tgggttcgta ttcaagaggt
     8221 tcaagatttc cattgatttt tctttgttct tagagttgtt tgagatgaca aagttagcaa
     8281 cttgtgttgt ttggtttttc ttgatgaagt tagtgattgg tttgatttgg atatctttgt
     8341 tggcaacacg tgaaagcaag ctgttaccgt agtcagctgg tcctactgtt tcttcacgaa
     8401 cgaaccaagt atcttgttga aggtcaaatg aagtgtcact tgttgcgacg tcttttggaa
     8461 tgtagccagc ttcatagaat ttgtgaagag tcttcaagtg ttctttgaaa cgaggcactt
     8521 cgtaacggtt tacaacttta gtagtatcgc cttcaaggtc aataacgaat ggaagcccgt
     8581 ttgctactgg gtagtcaaag ttatcagatg ggatgaaaac tttaccaata gcaaatggta
     8641 ctacgtctgg agctttttct ttgatttgtt tcaagactgg ctcaagagtt tcgtaagaag
     8701 taacacctga aatatcgata ccatatttgg caaggagagt tccgttgaag gcaaagtttt
     8761 gagatgatgc tacgttggct gcaactggta cagcgtaaat cttaccattt acagtattac
     8821 ccttgatata agctggatca agtgccttgt aaaggtcttt accttctttt ttgtacaatt
     8881 ctgtcaagtc agcataagca cctttttgag catttacaat atagttatct gcaaaggcga
     8941 tatcatagtt ttcaccagat gatgtgataa ctgacatttt cttaccatag tcaccccagc
     9001 caaggtattg gatatccaat ttggcaccaa ctttttcttc gatgattttg ttggcatttg
     9061 ctaacaattc atccaagtta tctggtttgt caccgatttg gtacattttg ataacaggtt
     9121 tgtcacctga atcagcagct tttttgctgt tacctgtcaa atttccacaa gcagcaagac
     9181 ctgcagccaa agcgactaca ctagcagatg caaaagcata ttttttccag tttttcatga
     9241 taaaaactcc tttttttatt tttaaactta taaacaatgt aatgatctta acttcacgca
     9301 agggaagttt ggaaacaagt gctattcttt cacaccaccg atagtcaaac cttttacaaa
     9361 gtagcgttgg aagaatggat acaaaattgc gattggaagg gttgctacca caaccatggc
     9421 catacgacct gtttctttcg gtagagcaac tcccagttga ccagtcaagc cgaccgcttt
     9481 ggcaatgtag tccatatttt gttggatttg catgagcaaa tattgcaatg gatacaagtt
     9541 gtcactcttg atgtaaagaa gggcgttgaa ccagtcattc cagaaaccaa gagctgtcaa
     9601 aagcgtgatg gttgcgatac ctggtagtga caatggcaaa cagatttgga agaaaatccg
     9661 ggcctcactg gcaccatcga tacgagccga ttcgagaatg gcttctggaa tggtcttctt
     9721 gaagaaggaa cgcatcaaga tgatattgaa tggtgagaga agcattggaa caatcaaggc
     9781 ccaaacagtg tcaccaagct gaagtacacg ggtcaccatg atataacctg gtaccaaacc
     9841 agcgttgaac aacatactga gaaggacgaa gatggtaaaa aatctgcgat acttaaaggt
     9901 tgtccgtgaa atagcgtagg cataggttgt tgtgataaag acatttgtca atgtcccaac
     9961 tacggttaca aagacagaga tgaagagggc ttgtaggatt ttatccttaa actgtgccaa
    10021 aaactcaaaa ccgtctaagc caaattggga tgggaagaag ctatagccgt attggaggag
    10081 gcttttctcg tctgtcactg aaataatgat aacgaataca aaaggtagga tacaagagag
    10141 ggcaatcaaa cccgaaatga tactgaagaa gatatctgct ttcttactga aggagtgaat
    10201 gccgacatta tcaatttttt cttttttaat tttctttttt gccatattct cctcctttct
    10261 agaacaaagc tgagtttgga tcgactcgtc ttgcaagcaa gtttgatagg ataaccagaa
    10321 tcaaaccaac aacggattgg taaagaccgg ctgctgcagc cataccgata tctgctgtct
    10381 gagtcaaacc attaaaaaca tatacgtcca aaacgttggt tacattgtaa agctgaccag
    10441 cattgtgtgg gatttgatag aagagaccga agtctgcgcg gaagatattt ccgactgcaa
    10501 ggatggtcaa tacagttaca agcggagtca actgaggaat ggttacgttg cgaatacgtt
    10561 gccacttgct agctccgtcc actgtcgctg cttcgtagta ggttggatca attcccatga
    10621 tcgtcgcata gtacatgaca ctgctatatc caaaaccttt ccaaatacct aggaaaagta
    10681 ggagataggg ccagatgccc aggtcagcgt agaaattgac ttctttgaga ccaagacttt
    10741 ccaatagatg attgaacacc cctttatcaa tatttaggaa ggcatctgta aagaaactga
    10801 tgataaccca agacaagaag taagggaaca acatagaagt ttggaaaatc ttcaccattc
    10861 tcttagaacg gagctcgctg aggataatgg caatccctac agatacaact aaacctagaa
    10921 agataaagcc aagattgtag aggacagtat ttcgtgtgat aataaaggcg tctcttgaac
    10981 taaataagaa tctgaaatta tcgagtccga cccatttact atttatgata ctatctatga
    11041 aaccattact ggtcatgtgg tagtctttga aggcaaccac gttcccaaat actggaatgt
    11101 aaaagaatag aatcaaccag agtgcccctg gcaaaaccat caagagaaag atccagttgt
    11161 ctctcaatgt ttttgaaaac tttttcataa tttcctccct ttttattttg atatccatct
    11221 aaaaattctt ttttagactt ttgataacga ttacattatt agtatactcc tatttgcagg
    11281 ttaggttaaa ctcctaatta tagaaaaaat tccacaaatt atgtagcaga tttaaaactt
    11341 tatcaccact atcaaacaaa tgtcctaaat caattgttta ttttatctct attagcccag
    11401 tgatggcgtc actctgttat aagcatccaa caacggggta tactgaaaaa tctccagact
    11461 agggaactca gcgatagttc ctaatctgga gatttttaat atgttattag gcgtttgctt
    11521 tcaacttagc aataacctct ttaagattat caatcaactc tgcttcagta tgctcagagc
    11581 ctttttcatc tgccaagaac aaaactgctt tttgaagttc ttcttgagag ttttcaagga
    11641 catccttatc tgctgtttct ttgcccgctg tttgtcctcc atccttagtt gctgactggc
    11701 tttccttaat ggactctagg gaagcaatgg catctttgac tgtttgcaag atatcacgta
    11761 aaccttgctc tgtcaaacta tcatctgtaa gagctttctt agcctctgcc aaaaccagac
    11821 gtgctgaatc tgtggtagga ttcgatacac ctgtcaatga tctcaaaaga ttttctaagg
    11881 tttgagtctg cttactaata ctagactaaa atcaaaaagc attacataac agtgatatga
    11941 aatcaactaa agaagaaatc caaaccatca aaacactttt aaaagactct cgtacagcta
    12001 aatatcataa acgccttcaa atcgttctat agtaaaatga aacaagaaca ggacaaatcg
    12061 atcaggacag tcaaa
//
LOCUS       DAVLXF010000060        11883 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLXF010000060 DAVLXF010000000
VERSION     DAVLXF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.060"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..198
                     /locus_tag="VYO12_001517"
     CDS             <1..198
                     /locus_tag="VYO12_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588091.1"
                     /translation="NTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDLLADD
                     SLAGDIILLWRIQFGTFTTET"
     gene            680..892
                     /locus_tag="VYO12_001518"
     CDS             680..892
                     /locus_tag="VYO12_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588092.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            976..1779
                     /locus_tag="VYO12_001519"
     CDS             976..1779
                     /locus_tag="VYO12_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1588093.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGNGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(1835..3376)
                     /locus_tag="VYO12_001520"
     CDS             complement(1835..3376)
                     /locus_tag="VYO12_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1588094.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(3378..3860)
                     /locus_tag="VYO12_001521"
     CDS             complement(3378..3860)
                     /locus_tag="VYO12_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1588095.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(3860..4945)
                     /gene="rlmN"
                     /locus_tag="VYO12_001522"
     CDS             complement(3860..4945)
                     /gene="rlmN"
                     /locus_tag="VYO12_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV1588096.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMTIGARHITVSTLGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDSVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(4966..5496)
                     /locus_tag="VYO12_001523"
     CDS             complement(4966..5496)
                     /locus_tag="VYO12_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1588097.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(5652..6257)
                     /locus_tag="VYO12_001524"
     CDS             complement(5652..6257)
                     /locus_tag="VYO12_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1588098.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(6430..7467)
                     /locus_tag="VYO12_001525"
     CDS             complement(6430..7467)
                     /locus_tag="VYO12_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1588099.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(7501..8436)
                     /locus_tag="VYO12_001526"
     CDS             complement(7501..8436)
                     /locus_tag="VYO12_001526"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1588100.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(8989..10179)
                     /locus_tag="VYO12_001527"
     CDS             complement(8989..10179)
                     /locus_tag="VYO12_001527"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1588101.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(10394..11737)
                     /locus_tag="VYO12_001528"
     CDS             complement(10394..11737)
                     /locus_tag="VYO12_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1588102.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
BASE COUNT         3541 a         2602 c         2188 g         3552 t
ORIGIN      
        1 aatactattt tagaaaaatt ttataaagaa caccaagtca aacccttcat atcgcctgaa
       61 cgagatttgg atacttggct tctaagtccc aaacccgttc ccaagcgaaa tatggactta
      121 ttagcagatg attcactagc aggagatatc attttactat ggagaatcca gtttggtact
      181 tttaccactg aaacatgatt ttaaaaactc ccattcgaat atgattcaaa attcgcttag
      241 aatcaatgtt ttcccacttt ttcaaaaaat aaattttcat ctaatttaaa taactttcaa
      301 ttaagtgtga attttgctta aaatcattaa aaaacacccc atggtgtgaa ccatgaagtg
      361 ttgtaaatgc tgtgttgtag ctagttctta gcgtttagag aactggctag ctttacgagc
      421 tttcttaaga tctggttccc taaatacttt gagtacactt atggcattga tgccccaaaa
      481 catctaaaaa ctctggtaga aaaagggtat gctcttgtcg aaacttcttt tgactcactt
      541 gaccatctaa ataataccat gaaaaagaat atcctgaaga agaagaaggg agttactgga
      601 ctttctaaaa tgaaatcttg cgacctggat caagcccttc atgagcattt ttcagaagaa
      661 gaattagctg gtcactttca tgtccttcta tggacttttt tacaatggca ttgctatcac
      721 acccaatacc tatctaagcg cctggttcgt aaactttatt gcagctcttc ctctaaattt
      781 cctaattgtt gaaccaattg cccgttttat actaagttct tttcagaaac catttactgg
      841 ggaagaagtt gaagattttc aagatgatga tgaaatccca actattatct aagccagttc
      901 tgtaaactac taatatttga aatccacttc cttttagggt acaatggtta taaatgaatt
      961 tttgagagga tcagaatgaa aaaactagca acccttcttt tactgtctac tgtagcccta
     1021 gctgggtgta gcagcgtcca acgcagtctg cgtggtgatg attatgttga ttccagtctt
     1081 gctgctgaag aaagttccaa agtagctgcc caatctgcca aggagttaaa cgatgcttta
     1141 acaaacgaaa acgccaattt cccacaacta tctaaggaag ttgctgaaga tgaagccgaa
     1201 gtgattctcc acacaagcca aggtgatatt cgcattaaac tcttccctaa actcgctcct
     1261 ctagcggttg aaaatttcct cactcacgcc aaagaaggct actataacgg tattaccttc
     1321 caccgtgtca tcgatggctt tatggtccaa actggagatc caaaagggaa cggtacaggt
     1381 ggtcagtcca tctggcatga caaggataag actaaagaca aaggaactgg tttcaagaac
     1441 gagattactc cttatttgta taacatccgt ggtgctcttg ctatggctaa tactggtcaa
     1501 ccaaacacca atggcagcca gttcttcatc aaccaaaact ctacagatac ctcttctaaa
     1561 ctccctacaa gcaagtatcc acagaaaatt atcgaagcct acaaagaagg tggaaaccct
     1621 agtctagatg gcaaacaccc agtctttggt caagtgattg acggtatgga tgttgtggat
     1681 aagattgcta aggccgaaaa agatgaaaaa gacaagccaa ctactgctat cacaatcgac
     1741 agcatcgaag tggtgaaaga ctacgatttt aaatcttaaa aaccaaaaaa atacagtatc
     1801 cacattcggt actgtatttc ttttactctc attcttaagt taaattatta aaatcccata
     1861 tttggtctat ccagccttca taaaagtctg gctcgtggca gaccataagg atagatcccc
     1921 tatattcttt gagagcgcgt ttgagctcat cctttgcatc cacatccaaa tggttggtcg
     1981 gctcgtccag cactaaaacg ttgttttcac gattcatcaa gagacagaaa cgaaccttgg
     2041 cttgctctcc ccctgataat acttgaatct ggctttcaat atgtttggtt gtcaaaccac
     2101 aacgggcaag ggctgcacgg acttctgctt gattaagggc aggaaaggca ttccagacag
     2161 cttcaagagg agtttggcga ttaccgcctt ctacttcctg ctcaaaataa ccaagttcta
     2221 aataatctcc acgctccact tccccagcga ttggcgagat aatgcctaag agactcttca
     2281 agagagttgt ttttccaata ccattagcac caataatcgc aaccttttga ttgcgttcga
     2341 aggtaagatt taaaggctta gtaagaggac ggtcgtaacc aatttgcaag ttcttggctt
     2401 ggaagataaa gcgccctggt gtacgagctg gtttgaaatc aaaggatggt tttggtttct
     2461 cactttggag ttcgataata tccatcttat ccaatttctt ttgacgagac atagccatat
     2521 tacgagttgc aacacgggct ttattacgag ccacaaagtc cttgaggtct gcaatctctt
     2581 tctgctggcg ttcgtaggct gcctctagct gagatttctt catagcataa acttcttgga
     2641 actggtagta gtcaccagag taacgcgtca gctgttgatt ttccacatga tagacaatat
     2701 taataacgtc attgaggaat ggaatatcgt gcgaaatgag aacaaaggca ttctcatagt
     2761 tttggagata gcgcttgagc caatcaatat gctcagcatc caagtagttg gtcggctcgt
     2821 ccaacagcaa gatatcaggc ttttcaagga gaagttttgc caaaagcacc ttggttcttt
     2881 gcccacctga caaagaagtt acatccgtat ccatgccaaa gtccataaca ccaagagcac
     2941 gcgctacttc gtcaatctta gcatccaagg tatagaaatc acgactctcc agacggtctt
     3001 gaagttctcc tacttcttcc atgagagcat caacatccgc gccgtcttca gccattttca
     3061 tatagaggtc attgatacga gcttcagctt taaaaagctc atcaaaagcc gtacggagaa
     3121 catcacgcac cgactgtctt tcagcaagga cagagtgctg atccaagtaa ccagccgtca
     3181 catatttgga ccactcaacc tttccttcat ctggcagcat tttaccagtc acgatactca
     3241 taaaggttga ttttccttca ccattggcac cgaccaggcc gatatgttct cccttgagga
     3301 gacggaagga cacatcttca aaaattgcac ggtcaccaaa accgtgactc agatttttaa
     3361 cttctaaaat actcatttta attccttatc ttgtttttat gtaatcgttt ataaaggagc
     3421 caagccagat agccacccaa agtgttggtc cacaaatcat caatctcaaa gacgcgattg
     3481 aaatcaaaga aaaagtccaa gattaattgc gtacactcga ttccaagact cacaagaaaa
     3541 ctaaaaagaa ggaccttttt tgttttccgc aaatttggaa atagataaag gagttggaaa
     3601 atcagaggaa aaaacaagaa gacattgagg acattttgta aaaaaatcca acataattgt
     3661 ccaatgtcac tcacttcgcc cagtttccag agagaattga aaggagtcaa aagaaaaacc
     3721 aggcgtccaa gatgctgaat acctggagtt cccactccca cggtagattg ttcttgagga
     3781 gtaaagcaaa aacagacaat gcaaatgcta tagaaaatga ctccccagac caaaatatga
     3841 ttataagtct tcttcatcat taaggattta ccgctgcgac tgccttctgg cggtcacgtt
     3901 tcattgtgtt agagcgcaat tgtccacaag ctgcgtcaat atctgtacca tgctcttgac
     3961 gaaccacaca gttgacccct tttttcttaa gcgtatcata gaaagccaac acgcgctctt
     4021 tgggactacg gctatattgg tcatgctcac taactgggtt ataaggaatc aagtttacat
     4081 aagacaattt cttgatgttc ttgagcaatt cagtcaattc caaggcttgt tctacactgt
     4141 cgttgacttc attaagcatg atatattcaa aggttacacg acggtttgtt gtctcaatgt
     4201 agtattcaat agcagcaaag agtttttcaa tcggaaaggc acggttaatc ttcatgatac
     4261 ttgaacgaag ttcattgtta ggtgcgtgaa gagacacggc aagattgacc tgaacccctt
     4321 catcagcaaa gtcacgaatt ttatgagcca aacctaaggt tgaaaccgtg atgtgacgag
     4381 caccgatagt cattccttta tcatcattga tagtacgaaa gaaattcaag acattgttgt
     4441 aattatcaaa gggctcaccg attcccatga caacgatatg gctgacgcgt tcatcctgac
     4501 cacgctcatc aaagtatttc tgaaccagca tgatttgcgc tacgatttca ccgttattga
     4561 ggtcacgttg cttcttaatc aaaccagagg cacagaaggt acaaccgata ttacagccga
     4621 cctgagtggt cacacagaca gataaaccat agtgttgacg catgagtaca gtctcaatta
     4681 acataccgtc gggcaattca aagagatatt tgactgtacc atcagcagac tcttgcacaa
     4741 tacgttgttt caagggattg accacaaact ggtcattgag cttagcaatc aaatccttgg
     4801 aaaggttggt catttcttca aatgactgca cacgtttacg gtagagccat tcccagattt
     4861 gatctgcacg gaatttcttt tctccctgct ccaataccca ttcctgcatg gtttgatgtg
     4921 ccaaactatg aattgagggt ttcatttctt ctccttattc tctactcact tctgacgaat
     4981 gacaaaatga cgttgtccct tgtcctcttt ctgagaatgc tggtctttct gacgacgtct
     5041 attcttctta tctgcattcg actttcgttt agtttgagtc ggtttctttc cttttctaga
     5101 aggtgtttct tcttccgtct tacgcatttt cttgtcaaat gatgctcgct taggggcttc
     5161 attttctaag acaaaatagg cacaaccata actacaatac tctaaaaggt agtcttgtaa
     5221 acgactgatt ttttcaagtt tttcttctgt tcggtcatcc ttgtaaaaac ctcgtaggcg
     5281 aagctgttcg ttgctccagt cccccacgat ataatcaaac ttggttaata cttctgaaaa
     5341 acgctgatta aaagtcgtca catcaaaggc atccttgata ttttcaacca aggaaaaagc
     5401 tatcccttcc gtttcgacct tgtccccgtg taaatggaac tccggaccag gaaacttgtt
     5461 atagttgtat aattcaggtg caatttcttt tcgcatagat atcctttttc cacgattact
     5521 taatacttta ttctaccata atttctagca gttagcacgt ttctcataaa aatgaaaaaa
     5581 gtctgacgat tttgtcagac cagaatctta taacctaaaa agagaagaac aattcttccc
     5641 tccaactatc attatttagc agctgcgtac aattcatcta ctttattcca gttgattact
     5701 gaaaagaaag ctttgatgta gtcaggacgc acgttgcggt atttcacgta gtaagcatgt
     5761 tcccaaacgt ccaagcccaa gattggtttt ttaccttctg agattggtgt gtcttggttt
     5821 gctgttgaag tcacttcaag tttcccttct ttgttgacaa ccaaccatgc ccaacctgaa
     5881 ccaaaacgag ttgttgctgc tgcagtgaag gctgcttgga attcttcaaa tgaaccaaat
     5941 gttgcatcga ttgctgctgc cagttctgct gaaggagctg ttttctcggg agtcatcaat
     6001 tcccagaaaa gagcgtggtt caagtgtccg ccaccattgt tgataagtgc ttgacggata
     6061 tcagctggga tagattctac atcagcaagc aaggcttcaa ggtcttcacc gatttcaggg
     6121 tgtttttcta aagctgcatt ggcattgttg acataagttt gatggtgttt gtcatggtgc
     6181 aaatgcattg tttccgcatc gatgtatggt tccaaagcgt cgtatgcata tggaagttct
     6241 ggtaagataa tagccatctg taatacctct ttttctttct atatgaaaat gataacgcaa
     6301 acattcactt ttttcaagtt ttttgcttat agataaggaa atcactgaca tactttctaa
     6361 taaaataagc aaatcagcaa gaaacccacg acaagataat cctgacgcgg gttgtagttt
     6421 caacaaatgt caattgacct gactagcaat ctgtaagagt gccttttcaa aaaggaaacc
     6481 tttttcataa agacctgtct taatctgata gtctgtctca atcaaatagg aaatagcttg
     6541 cttcagaaag ctcaaagaaa gtcctctcga atctcttaat gcaaacttga tttgataagg
     6601 atttgggtta cgtcccagat aactacctaa actacttgca atctgcgatt ctgtttggcc
     6661 agactccgcc aaaatcttca cctgagtaaa agtccgaaat tgtcccagca tgactgcaat
     6721 cagtttgatt tcatcttccc cttgcaaggt caagtctctc accaaatcgc gcgcctgatc
     6781 catcttttta gtcagaataa actgagttaa atcaaaaata ttgtcctgca aggtcttggg
     6841 aattgcgtta acaatatcct cttcctcgat aacagaatcc gccttatagg actgtaaaaa
     6901 gagaagattt ttctggattt cgctaaattg aaaccccgac ttgatgagga gattttcaaa
     6961 agaatgattg gtaaactgca gaccttgttt ctgactccac ttttggaaat actggcgcaa
     7021 ttcttgttct tttacttcta ctgcatcgaa gaccttggca tcacgcttaa gtaatttgac
     7081 taaccgtctt ttgctatcca gctttccttc tgcaaagatt atcaacttgg ttgttggaga
     7141 aggattgtca aggtattcct caaatgactt aagctcatca tctgtcaaaa agcgtttctt
     7201 agcagtcgtg atatccataa aataatctaa tatcacgatt ttctcatccg caaagaaagg
     7261 aaggctgacc aactccagtt ccacatcctt gtaaactact tctttcatat caaagtaggc
     7321 aaagttgagg tcagcagaat catacccaat ctgtttcaac acttgactct tcatcacttc
     7381 aaactgaccc tgatctgtcc ctgtaaatag gctcaggctc ggtaaatttg ataaagtcaa
     7441 cttctgactt tcttcaatgg ctagcatcgt ctctcctttc ttcagatttt tcgatttaat
     7501 ttagtcaata tagcgcaatt tcccacggaa atcttctaag ctctcgtagc ctttttccac
     7561 catgattgct ttcagttcat tggtaatgcg gtcaaaagca ctgacgcctt ctttgtgaag
     7621 ggtcgttccc acctgcacca tacttgctcc acagaggatg tgttcaaagg catctcgacc
     7681 agtcagaacg ccacctgttc cgataatttg gatttgagga tttaaacgtt gataaaaggc
     7741 gtgaacattg gctagagcag tcggtttgat gtattctcca ccaattccac caaaaccatt
     7801 cttaggccga ataacgacag attcgtcttc tatatagagg ccgtttccga tagagttaac
     7861 gcagttgaca aacttgagcg gatatttgtt gaaaatagct gccgcttgat caaagtgaac
     7921 aatatcaaaa taaggtggca atttaattcc aagaggtttg gtgaagtaag caaacacttc
     7981 tgccaaaatc cggtctgttg tctcaaaatc ataggcaatc tgaggtttac ctggaacatt
     8041 tggacaggaa agatttagct cagtcagacc acgaaaatca ctctcttgga cttttttcaa
     8101 aatagtatgg gtttcctctg gagacatgcc gaccagagat aagaagaaag ttcggttcga
     8161 ctctttttcc tgcaaatcta aaagataatc caaataatag tctaagccat tatttggcaa
     8221 gcccatagag ttgatggaac caagtggaac atcttggtag cgtggctcag gattcccctg
     8281 acggaagtcc aaggtcgctg tcttagtaac aaaggttcct gccgctgagt ttttgacctc
     8341 ttctaactcc tctatcgtca tacaagccac acctgctgca ttcatcaagc aattgtcaaa
     8401 ctcaaaacca gcaatttgtg ttttcgttga taccatgatt ctaatcctcc agatttcttt
     8461 tattgctaat attataccat aattttaatt ctaggtttac tagattctgt ggttctatct
     8521 atttattcgg aaaagaaagt ctagtacaga agtcatgcta tggaaacagc tagtgtatta
     8581 gaagtttgaa caaggcttgg gtaacagact tttgaatgaa gtccaagcaa caaggagaaa
     8641 tctcaaccat gagtccattc catgactctg ttgctctaga aaaccttgca atagtcaagt
     8701 gataacgaat aaaactttgg attgaaatat ccccaagatt atctattacg aggagaaatc
     8761 ttaaccttta tcatttttaa agaaatatag taaaatgaaa caagaacagg agaaatcgat
     8821 caggacagtc aaattgattg ctaacaatgt tttagaagtt gaggtgtact attctagttt
     8881 caatctacta tacatgtaaa tctctacttt cgcaaaatgg attttcagaa cagtatccca
     8941 gtatagttaa aaactataaa aaagagagga cgttcccctc ttaaaatctt attttactgc
     9001 ttctttcaaa gcatctacct tatccaaacg ttcccatgga agatcaatat ctgtacgtcc
     9061 catgtgaccg taagccgatg tttgacggta aattggacgc ttgaggtcca gcatttggat
     9121 aatccctgca gggcgaaggt caaagatttg acgagccgct ttttcaagtt gactttcagc
     9181 tactgttcct gtaccgaaag tatcgatacg aacagaaaca ggttgcgcaa caccgatagc
     9241 ataggccaac tgcacttctg ccttcttagc aaggcctgct gcaacgatat tcttggcaat
     9301 atagcgagcc gcataagagg ctgaacgatc caccttagtc gcatctttac cagagaaggc
     9361 accaccacca tgacgagagt agccaccata agtatctaca ataatcttac gaccagtcaa
     9421 acctgagtcc ccttgaggac caccgattac aaaacgacct gtcggattga taaagaattt
     9481 tgtcttatca tcaagataag aagatggaat aacttctttg atgaccttgt caatcacatc
     9541 ttgatggatt tgttcattag tggcctctgg atcatgctga gtagaaataa cgactgtatc
     9601 tacacgtacc ggacggtcat tttcatcgta ctcaactgta acttgtgatt ttgcatctgg
     9661 acggagatag ctaatttctc cagacttacg aagttctgcc agacgacgaa ccaatttatg
     9721 actgagtgca attggcaatg gcataagctc ttctgtttca tctactgcaa atccaaacat
     9781 gagcccttgg tcacctgctc caatcaagtc cagtggatct tgatcagcat ttccacgaac
     9841 ctccaaggct tcgttaacac cttgagcgat gtcaggagat tgttccacca aagatgggtg
     9901 tactcccacc gtctcagcag aaaatccata ttctgtattg gtataaccaa tctctgcaat
     9961 ggtatcacga accacacggt taatatccac ataggcattt gtagaaattt caccaaaaac
    10021 gtggacagaa ccagtatata cagctgtttc agcagcaacg tgcgcctctg gatcctttgc
    10081 taaaatagca tccaaaatcg catctgaaat ttggtctgca atcttatccg gatgcccctc
    10141 agatacagat tcagacgtga ataatttacg ttctgacata aaaatgtccc cccttaaaaa
    10201 atattgttat agagttacaa agtactgata ggaaaactct tcgtaataaa tacctaccat
    10261 cttatcggga ctatataact ttatcattat accattttta atcgcaattt gcagtcttta
    10321 atgtagtgtt tcggtttaga tagttcgttc tcataaagca aaaagttgga gctcagtact
    10381 ccaacaattt ctattaaaat gtttgacgtt tagctttacg ctctgcacga ccgcgagcgc
    10441 gattttcagc acgcttggtt ttacggcgct tttcatcaac cgcccattga attttcttct
    10501 tataacccgg tttgactttt ttctttttct ttttaaccaa accaatcatt tcgatatcaa
    10561 gtttatcttg ttttttctca cggttggcac gacgatcacg gtcataggta tcttgaaatt
    10621 ccccgtcttt gaccatctta ggactaaact tgattcccaa tttctccaac tcacggatat
    10681 ccgagtcatc acttggctga taaagggtaa tagctgtacc tggtaggcca tttcgtccag
    10741 tacgaccaac acggtgaaca aaaaaagata agtcttgcgg aatggcatca ttgatgacat
    10801 ggctgacacc ttcaatgtca atcccacgtg ctgccaaatc tgttgcgaca atatactcaa
    10861 aatccagatt ttgcacctga ttcatgattc gcttgcgttc acgaggggca atatcgccat
    10921 ggatttttgc aaccttcaag ccttgagcag tcagatatga atgcaattca tcagcacgcg
    10981 ttttagtgtt aacaaaaatc attgccaaat acggctgcat caactgagtc aactggtaaa
    11041 tttgagcatt cttatcatgt cccttggtcg aaatcaacca attatcaatg gtgtcagaaa
    11101 taaccgtttt ggtcttaatt ttctccataa caggattgga taagtatttt ttcaagaatg
    11161 gttgcagttt ttgtgggata gtcgctgaga agaccatgaa ttgcaagtct tttggaagac
    11221 tgccagcaat cttatcaaca gtttccaaga atcccatatc caaggtcata tccgcttcat
    11281 caacaacaaa tgtcttggct ttatgaatag ctaaatcacc agatttaacc aagtcgtaga
    11341 tacggcctgg tgttccaata acaatatgag gctgattgct tgccaatttc tcaatctggc
    11401 gagccttatc cgtaccaccc acataattaa ccacacgaac ttcgacatct gagtgagctg
    11461 aaatctgacg cgctacttgg taaatttgag tagccaactc acgactcggt gcagtaatca
    11521 ctgcttgtac actatcgcta gcttcatcta attgctggaa aatcggtaac aagaaagtat
    11581 gagtcttacc tgaacctgtt tttgattctc ctactaggtc acgacctgcc aaaacaatag
    11641 gaatcaactt gtcttgcacc tctgttggag ttgtaaattt taactcctcc aaggcttctc
    11701 taatatagtt tttaaattga aatttcgtaa atgacataac atcctcgatt ctatctatct
    11761 tatcaattat accatatttt attccattac agtagtctca cttatttagg ctatttccag
    11821 tagcttctct agtaagaaaa ggctggaatt ttatacccca attattcacc cctaatctaa
    11881 aaa
//
LOCUS       DAVLXF010000061        11766 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLXF010000061 DAVLXF010000000
VERSION     DAVLXF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11766
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.061"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(127..1758)
                     /locus_tag="VYO12_001529"
     CDS             complement(127..1758)
                     /locus_tag="VYO12_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1588103.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2051..6961
                     /locus_tag="VYO12_001530"
     CDS             2051..6961
                     /locus_tag="VYO12_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1588104.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTPAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGKWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEPKKDYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHETSTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGSALAPLLEKNK
                     ALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNKVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            7051..8247
                     /locus_tag="VYO12_001531"
     CDS             7051..8247
                     /locus_tag="VYO12_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1588105.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8383..8910
                     /locus_tag="VYO12_001532"
     CDS             8383..8910
                     /locus_tag="VYO12_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1588106.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            8987..9343
                     /gene="glnR"
                     /locus_tag="VYO12_001533"
     CDS             8987..9343
                     /gene="glnR"
                     /locus_tag="VYO12_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1588107.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9380..10726
                     /gene="glnA"
                     /locus_tag="VYO12_001534"
     CDS             9380..10726
                     /gene="glnA"
                     /locus_tag="VYO12_001534"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1588108.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTVIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10822..10941)
                     /locus_tag="VYO12_001535"
     CDS             complement(10822..10941)
                     /locus_tag="VYO12_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1588109.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11429..11766
                     /locus_tag="VYO12_001536"
                     /pseudo
     CDS             11429..11766
                     /locus_tag="VYO12_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3766 a         2155 c         2549 g         3296 t
ORIGIN      
        1 agaggtgtac tattctagtt tcaatatact atatatctgc agaatactct ttcagtttca
       61 ttcagcctag tcttgcttag gaaaaagtaa gaagccatcc aattatgatg gatagcttct
      121 tggttcttag atttgttctg caaaaacctt ttcagcaagg ttcatagcgt ggtctgatac
      181 acgagtgtag tgtgagatga tgtcgataaa gttgacccca gcttgtgttg aacattcgcc
      241 tttgttgagg cgtttgatgt gggtttttct gagaacacgt tctatcttat tgattgcttc
      301 atgacgttct acaagactgc gtgctttttc aatatcattg ttttccacac tatccagagc
      361 atctttgata aagtcactag tttggcggta aacttcctct aattctttca aggcggcatc
      421 agaaaattca acatttttcc gttgaagata gtcagtcaga ttgagtagag cctccgtgtg
      481 gtctccaatc cgttccaaat cacgggagga atcaaggata ttggtgagca cttcactttc
      541 tttttggctg agagcttcgc ttgaaagggc aatgagataa cgtgttaatt gctcatcgat
      601 ggtgttaatt gcttcttcgg ttttatgccc tttttcagca actttttcat ccaagtcaat
      661 gatgtactta taggaaaggt caaaggcttt agcagcgtag tttcctaagt gcaagagctc
      721 ttttttagca tttcctagag cgatagatgg ggcttgtttg atgaaatgtt catcaagata
      781 taagggttcg tatttgacaa cctcgtcctc tccaggaata atcttggtta caaagtaagc
      841 cagagctccg ataaatggaa attggacaat ggtgttggta atattaaagg ttccgtgagc
      901 aaaggcgatg gtcatttccg gtgctagatt tagcgtagct tcaaaccaat gaatcaggac
      961 agtaaaagga actagaaaaa taacgcagac gactgttccg ataacgttga aggcaacatg
     1021 agctcctgct acccgtttag ctgcaatatt agcccctaaa gaggcaatga tggctgtaat
     1081 ggttgtcccg atattgtcac caaatagaac tggcaaagct ccctgtaggt caattagatt
     1141 gccggcgtag aggttttgta aaatcccaat ggtagccgaa gaagcttgaa ttagcaaggt
     1201 caagccggta ccgacaaaga cacccaaaac aggattctta cttagctcaa tcatatagtc
     1261 cttaaagacc tgtaaatcct tgagtggagc cattgcgccg ctcatgagat tgagggcaaa
     1321 aaagatacca ccgacaccaa agaggatgcg tccgatatta ttgactgtcc gatttttcgt
     1381 aaaaaagaga cagacggcac cgataaagag cataggtagg gcatagttac ctaatttaaa
     1441 accgatgaga aaggatgtga cagttgtccc aatattagca cccatgacaa tcccgatagc
     1501 ctgacgtaag gttaagagac cggcactgac caggccgact gtaataactg ttacaccaga
     1561 actagactga attagagcag tcatcccaat accaaccaga actccaaaga aaggattact
     1621 agtatatttg tcaatgtaaa aacgaaggcg atctccagca gcttgttgca aaccgtctcc
     1681 catggtcttg atgctatata agaatagccc cagaccacct aaaaagtgaa ataaaatttc
     1741 ctgccaatta atggacattt ctttttcctc cgaaaaataa tcgcggaata tctcctattc
     1801 tattttaaag gataaaagta aatctaacaa gtgttaatgt caagtttgta aaagaaaatt
     1861 ataattgata agtgtattat ttttaagagt ttttaacctt attttagatt tgtgcaaaca
     1921 gttattgttt tttgtctaaa aatgttttag gataaaaatg tgattatcat ggttctagag
     1981 tttgataaaa aactacagaa tataaagcgc tttctttaaa acaacaaaat caatctttta
     2041 ggaggagaaa atgaagaatc cattttttga aagacgttgt cgttacagta ttcgtaagtt
     2101 atcagtagga gcctgctcgc tgatgattgg tgctgtttta tttgctggtc cagccttggc
     2161 tgaagaaact gcagttcctg aaaatagcgg agctaataca gagcttgttt caggagagag
     2221 tgagcattcg accaatgaag ctgataagca gaatgaaggg gaacatgcta gagaaaacaa
     2281 gctagaaaag gcagaagcag ctagcgcagc taaaccagag gaaaaagcag gtgaggtggt
     2341 tgcagaaaca ccatctgcag aagcaaaacc taagtctgac aaggaaacag aagcaaagcc
     2401 cgaagcaact aaccaagggg atgagtctaa gccagcagca gaagctaata agactgaaaa
     2461 agaagtccag ccagatgtcc ctaaaaatac agaaaaaaca ttaaaaccaa aggaaatcaa
     2521 atttaattct tgggaagaat tgttaaaatg ggaaccaggt gctcgtgaag atgatgctat
     2581 taaccgcgga tctgttgtcc tcgcttcacg tcggacaggt catttagtca atgaaaaagc
     2641 tagcaaggaa gcaaaagttc aagccttatc aaacaccaat tctaaagcaa aagaccatgc
     2701 ttctgttggt ggagaagagt tcaaggccta tgcttttgac tattggcaat atctagattc
     2761 aatggtcttc tgggaaggtc tcgtaccaac tcctgacgtt attgatgcag gtcaccgtaa
     2821 cggggttcct gtatacggta cactcttctt caactggtct aatagtattg cagatcaaga
     2881 aagatttgct gaagctttga agcaagacgc agatggtagc ttcccaattg cccgtaaatt
     2941 ggtagacatg gccaagtatt atggctatga tggctatttc atcaaccaag aaacaactgg
     3001 agatttggtt aaacctcttg gagaaaagat gcgccagttt atgctctata gcaaggaata
     3061 tgctgctaag gtaaaccatc caatcaagta ttcttggtat gatgccatga cctataacta
     3121 tggacgttac catcaagatg gtttgggaga atacaactac caattcatgc aaccagaagg
     3181 agataaggtt ccggcagata acttctttgc taactttaac tgggataagg ctaaaaatga
     3241 ttacactatt gcaactgcca actggattgg tcgtaatcct tatgatgtat ttgcaggttt
     3301 ggaattgcaa cagggtggtt cctacaagac aaaggttaag tggaatgaca ttttagacga
     3361 aaatgggaaa ttgcgccttt ctcttggttt atttgcccca gataccatta caagtttagg
     3421 aaaaactggt gaagattatc ataaaaatga agatatcttc tttacaggtt atcaaggaga
     3481 ccctactggt caaaaaccag gtgacaaaga ttggtatggt attgctaacc tagttgcgga
     3541 ccgtacgcca gcggtaggta atacttttac tacttctttt aatacaggtc atggtaaaaa
     3601 atggttcgta gatggtaagg tttctaagga ttctgagtgg aattatcgtt cagtatcagg
     3661 tgttcttcca acatggcgct ggtggcagac ttcaacaggg gaaaaacttc gtgcagaata
     3721 tgattttaca gatgcctata atggcggaaa ttcccttaaa ttctctggtg atgtagccgg
     3781 taagacagat caggatgtga gactttattc tactaagtta gaagtaactg agaagaccaa
     3841 acttcgtgtt gcccacaagg gaggaaaagg ttctaaagtt tatatggcat tctctacaac
     3901 tccagactac aaattcgatg atgcagatgc atggaaagag ctaacccttt ctgacaactg
     3961 gacaaatgaa gaatttgatc ttagctcact agcgggtaaa accatctatg cagtcaaact
     4021 atttttcgag catgaaggtg ctgtaaaaga ttatcagttt aacctaggac aattaactat
     4081 ctcggacaat caccaagagc cacaatcgcc gacaagcttt tctgtagtga aacaatctct
     4141 taaaaatgcc caagaagcgg aagcagttgt gcaatttaaa ggcaacaagg atgcagattt
     4201 ctatgaagtt tatgaaaaag atggagacag ctggaaatta ctaactggct catcttctac
     4261 aactatttat ctaccaaaag ttagccgctc agcaagtgct cagggtacaa ctcaagaact
     4321 gaaggttgta gcagtcggta aaaatggagt tcgttcagaa gctgcaacca caacctttga
     4381 gtgggggatg actgtccaag acacaagtct tccaagacct ctagcagaaa acatcgttcc
     4441 aggggctact gttatcggaa gtaccttccc agatacagaa ggtggagaag gtattgaagg
     4501 tatgttgaac ggtaccatta ctagcttgtc agataaatgg tcttcagctc agttgagtgg
     4561 tagtgtggat attcgtttga ccaagccacg taccgttgtt agatgggtca tggatcatgc
     4621 aggggctggt ggtgagtctg ttaacgatgg cttgatgaac accaaagact ttgaccttta
     4681 ttataaagat gcagatggta agtggaagct agctaaggaa gtccgtggca acaaagcaca
     4741 cgtgacagat atcactcttg ataaaccaat cactgctcaa gactggcgct tgaatgttgt
     4801 cacttctgac aatggaactc catggaaggc tattcgtatc tataactgga aaatgtatga
     4861 aaagcttgat actgagagtg tcaatattcc gatggccaag gctgcagccc gttctctagg
     4921 caataacaag gtacaagttg gctttgcaga tgtaccggct ggagcaacta ttaccgttta
     4981 tgataatcca aattctcaaa ctccactcgc aaccttgaag agcgaagttg gaggagacct
     5041 agcaagtgca ccattggatt tgacaaatca atctggtctt ctttattatc gtacccagtt
     5101 gccaggcaag gaaattagta atgtcctagc agtttccgtt ccaaaagatg acagaagaat
     5161 caagtcagtc agcctagaaa caggacctaa gaaaacaagc tacgccgaag gggaggattt
     5221 ggaccttaga ggtggtgttc ttcgagttca gtatgaagga ggaactgagg acgaactcat
     5281 tcgcctaact cacgcaggtg tatcagtatc aggttttgat acgcatcata agggagaaca
     5341 gaatcttact ctccaatatt tgggacaacc agtaaatgct aatttgtcag tgactgtcac
     5401 tggccaagac gaagcaagtc cgaaaactat tttgggaatt gaagtaagtc aggaaccgaa
     5461 aaaagattac ctagttggtg atagcttaga cttgtctgaa ggacgctttg cagtggctta
     5521 tagcaatgac accatggaag aacattcctt tactgatgag ggagttgaaa tttctggtta
     5581 cgatgctcaa aagactggtc gtcaaacctt gacgcttcgt taccaaggtc atgaagtcaa
     5641 ctttgatgtt ttggtatctc caaaagcagc attgaacgat gagtacctca aacagaaatt
     5701 agcagaagtt gaagctgcta agaacaaggt ggtctataac tttgcttcac cagaagtaaa
     5761 agaagctttc ttgaaagcaa ttgaagcggc cgaacaagtg ttgaaagacc atgaaactag
     5821 cacccaagat caagtcaatg accgacttaa taaattgaca gaagctcata aagctctgaa
     5881 tggtcaagag aaatttaagg aagaaaagac agaacttgat cgcttaacag gtgaggttca
     5941 agaactcttg gctgccaaac caaaccatcc ttcaggttct gccctagctc cgcttcttga
     6001 gaaaaacaag gccttggttg aaaaagtaga tttgagtcca gaagagcttg caacagcgaa
     6061 acagagtcta aaagatctgg ttgctttatt gaaagaagac aagccagcag tcttttctga
     6121 tagtaaaaca ggtgttgaag tacacttctc aaataaagag aagactgtca tcaagggttt
     6181 gaaagtagag cgtgttcaag caagtgctga agagaagaaa tactttgctg gagaagatgc
     6241 tcatgtcttt gaaatagaag gtttggatga aaaaggtcaa gatgttgatc tctcttacgc
     6301 ttctattgtg aaaatcccaa ttgaaaaaga taagaaagtt aagaaagtat ttttcttacc
     6361 tgaaggcaaa gaggcagtag aattggcttt tgaacaaacg gatagtcatg ttatctttac
     6421 agcaccacac tttactcatt atgcctttgt ttatgaatct gctgaaaaac cacaacctgc
     6481 taaaccagca ccacaaaaca aagtccttcc aaaacctact tatcaaccgg cttctgatca
     6541 acaaaaggct cctaaattgg aagttcaaga ggaaaaggtt gcctttcatc gtcaagagca
     6601 tgaaaatact gagatgctag ttggggaaca acgagtcatc atacagggac gagatggact
     6661 gttaagacat gtctttgaag ttgatgaaaa cggtcagcgt cgtcttcgtt caacagaagt
     6721 catccaagaa gcgattccag aaattgttga aattggaaca aaagtaaaaa cagtaccagc
     6781 agtagtagct acacaggaaa aaccagctca aaatacagca gttaaatcag aagaagcaag
     6841 caaacaattg ccaaatacag gaacagctga tgctaatgaa gccctaatag caggcttagc
     6901 cagccttggt cttgctagtt tagccttgac cttgagacgg aaaagagaag ataaagatta
     6961 aatatcgaaa aatcttgtga aatctttcct tatatttcca aagtgtgata taatagtttc
     7021 gaataaaata aataaaggag tttttgtaac atggcaaaac ttactgttaa agacgttgac
     7081 ttgaaaggta aaaaagtcct cgttcgtgtt gacttcaacg taccattgaa agatggcgta
     7141 atcactaacg ataaccgtat cacagcagct cttccaacta ttaagtacat catcgaacaa
     7201 ggtggacgtg caattctttt ctctcacctt ggacgtgtga aagaagaagc tgataaagct
     7261 ggtaaatcac ttgctcctgt agcagctgac ttggcagcaa aacttggtca agatgttgtt
     7321 ttcccaggtg tcactcgtgg tgctgaattg gaagcggcaa tcaacgctct tgaagatgga
     7381 caagttctct tggttgaaaa cactcgttac gaagatgttg acggcaagaa agaatctaaa
     7441 aacgatcctg aacttggtaa atactgggca tcacttggag atggtatctt cgtaaacgat
     7501 gcattcggta cagctcaccg tgcacacgca tctaacgttg gtatctcagc aaacgttgaa
     7561 aaagcagttg ctggtttcct tcttgaaaac gaaattgcct acatccaaga agcagttgaa
     7621 actccagaac gtccattcgt ggctatcctt ggtggttcaa aagtttcaga caagatcggt
     7681 gttatcgaaa acttgcttga aaaagctgat aaagtcctta tcggtggtgg gatgacttac
     7741 acattctaca aagcacaagg tatcgaaatc ggtaactcac ttgtagaaga agacaaattg
     7801 gatgttgcga aagctcttct tgaaaaagca aatggtaaat tgatcttgcc agttgactca
     7861 aaagaagcta acgcatttgc tggttacact gaagtgcgtg acactgaagg tgaagcagtt
     7921 tctgaaggct tccttggtct tgacatcggt ccaaaatcta tcgccaaatt tgacgaagct
     7981 ttgactggtg ccaaaacagt tgtatggaac ggacctatgg gtgtatttga aaacccagat
     8041 ttccaagctg gtacaatcgg tgtgatggac gctatcgtga aacaaccagg agttaaatca
     8101 atcatcggtg gtggtgactc agctgccgca gcgattaacc ttggccgtgc agacaagttc
     8161 tcatggatta gtacgggtgg tggagcatca atggaacttc ttgaaggtaa ggttcttcca
     8221 ggacttgcag ccttgacaga aaaataagat tttataaaca aatcaaagaa gagagggatg
     8281 aaagttcctc ttttcttttg cttaaaataa aaacgcttcc tctcaactat tactcataaa
     8341 aatcaccgat ttatgataaa atggaaatag aaagttgaga ttatgagtta ttttaaaaaa
     8401 tataaattcg ataaatccca gttcaaactt ggtatgcgaa cctttaaaac aggtattgct
     8461 gtttttctag ttctcttgat ttttggcttt tttggctgga aaggtcttca aattggtgct
     8521 ttgacagccg tttttagcct gagggagagt tttgatgaga gtgttcattt tgggacttcg
     8581 cgtattctag gaaatagtat cggtggactc tatgccttgg tcttcttctt attaaatacc
     8641 tttttccacg aagccttttg ggtgaccttg gtagttgttc caatctgcac catgttaacc
     8701 attatgacaa atgtagccat gaataacaaa gcaggggtta ttggtggtgt agcagctatg
     8761 ttaatcatta ccctatcaat tccaagtggt gagacaattt cgtacgtgtt tgtgcgtgta
     8821 tcagaaacgt ttatgggagt ttttgtcgca attatcgtaa attacgatat tgatcgtatt
     8881 cgtctctttt tagagaaaaa agaaaaataa tgttacattt tataacatta tcaattgacg
     8941 tttgtctttt tttagactat aatagacaga aagaaggaaa ttgtaaatga aggaaaaaga
     9001 atttcgccga aatatggctg tttttcctat cggcagtgtt atgaagttga ccgatctatc
     9061 ggcgcgtcag attcgttatt atgaagatca agagttgatc aagcccgatc gaaacgaagg
     9121 aaatcgtcgc atgtattcct tgaatgacat ggatcgtctg cttgaaatca aagattatat
     9181 ctctgaaggt tataatatcg ctgccattaa gaaaaaatat gctgaacgtg aagcgaaatc
     9241 caagaaagcg gttagtcaga ctgaagtacg tcgtgcactt cacaatgaac tcctccaaca
     9301 ggggcgtttt gcttcagtac agtcaccttt tggtcgcggt taggcaaccg caagtagtca
     9361 tagattaagg agaaaactta tgccaatcac agctgcagat attcgtcgtg aagtcaagga
     9421 aaaaaatgtt acctttattc gtcttatgtt ctcagatatt ttgggaacca tgaaaaacgt
     9481 cgaaattcct gctacagatg aacagttaga taaggtcttg tcgaacaagg ttatgtttga
     9541 tggatcttct attgaaggtt ttgtacgtat caatgagtcg gatatgtact tgtacccgga
     9601 cttggataca tggacagtct tcccttgggg agatgaaaat ggaagtgttg caggtctgat
     9661 ctgtgatgtt tatacaacag aaggtgaacc atttgcgggt gaccctcgtg gtaatttgaa
     9721 acgagctctt cgtcacatgg aagaagttgg attcaaatcc ttcaaccttg gtccagagcc
     9781 agaattcttc ctatttaagt tggatgaaaa tggggaccca acacttgaag tgaatgacaa
     9841 gggtggctac tttgatttgg cacctactga ccttgcggac aacacacgtc gtgagattgt
     9901 gaatgtcttg accaaaatgg gatttgaagt agaagcgagt caccacgagg ttgcggttgg
     9961 acagcatgag attgacttta agtacgatga agttctccgt gcttgtgata agattcaaat
    10021 ctttaaactt gttgttaaaa ccattgctcg caaacacgga ctttacgcaa catttatggc
    10081 gaagccaaaa tttggtattg ctggatcagg tatgcactgt aatatgtcct tgtttgatgc
    10141 agaaggaaat aacgccttct ttgatccaaa tgatccaaaa ggaatgcagt tgtcagaaac
    10201 agcttaccat ttcctaggcg gtttgatcaa gcatgcttac aactatactg tcatcatgaa
    10261 cccaacagtt aactcataca aacgtttggt tccaggttat gaagcgcctg tttacattgc
    10321 ttgggctggt cgtaaccgtt cgccacttgt gcgcgtacct gcttcacgtg gtatgggaac
    10381 tcgtcttgag ttgcgttcag tggatccaat ggcgaaccct tacattgcta tggctgttct
    10441 tttggaagtt ggtttgtatg gtattgaaaa taaaatcgaa gcaccagctc ctatcgaaga
    10501 aaatatctac atcatgacag cagaagagcg caaggaagct ggtattacag accttccatc
    10561 aactcttcac aacgctttga aagctttgac agaagatgaa gtggttaaag ctgctctcgg
    10621 agatcacatc tacactagct tccttgaagc caaacgaatc gaatgggcaa gttatgcaac
    10681 cttcgtttca caatgggaaa ttgataatta tttagacctt tactaatact aatatagaag
    10741 aaagattgcc tgtgggtaaa cagtcacaca gcagtctaag ccatcaaata cctataaccc
    10801 ttaaaatagc gatttcggtc gctatttatt tttcgaaaaa tcctcttgac tcttttgcga
    10861 attagattta tcatcagacg tgtagaagaa aatcaaggag tcactatgga aaaattcaat
    10921 ttcaaaaaca atatcgggca agaaaacaag ttacttcaaa tcgaaatcta caagtttact
    10981 aacttttgca agcttcaaaa ttacacatct gtaaatattt tttcgaaaga tatatttgaa
    11041 gccatagtaa attgagacta gaatagtaca ctacatctgc taaaaatttc tagaaattaa
    11101 tttaactttc ctaatcgatt tgtttacatc ttatttcaat ctactatatt atctcaataa
    11161 atgatgatgg cgactgtttt gtttgtgctt gtcgattttt gaatcctgat cgagcggaag
    11221 aatatttctg ccgaggttgt caaggggaaa attctgaaga tattgaattt tatgatgaac
    11281 aattacaggc agaaaaggtg gaggctttga atattcgatt ggaagtaaaa aattgaatta
    11341 tctacaatct catcaacaat aaacctattt caaccccatt atctataggc gtatttttac
    11401 gcctttttat atacttgaag cacataagaa acattatggg aagtttgctg atggaagcac
    11461 tcaagaaatt gatgttcctt atgatattcc tgatacttgg gagtgggtga ggataaaatc
    11521 aatttattgg aattttgggc aaaataagcc agagaaatcc tttaggtata tagatacgtc
    11581 tagtattgat agaaaaaaga acataatcaa ctacaaaaat ctacaatatc tttcacctga
    11641 acaagcgcct tcccgtgcta gaaaattagt ttcgcagaat agtgtcttat tttcaacagt
    11701 tagaccatat ctaaaaaata ttgctgtagt tagagaactt aaagagtatt tgatagctag
    11761 tacagc
//
LOCUS       DAVLXF010000062        11527 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLXF010000062 DAVLXF010000000
VERSION     DAVLXF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.062"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            28..891
                     /locus_tag="VYO12_001537"
     CDS             28..891
                     /locus_tag="VYO12_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588110.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEAKELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1274..1894
                     /locus_tag="VYO12_001538"
     CDS             1274..1894
                     /locus_tag="VYO12_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588111.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1980..2423
                     /gene="tsaE"
                     /locus_tag="VYO12_001539"
     CDS             1980..2423
                     /gene="tsaE"
                     /locus_tag="VYO12_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1588112.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2413..2931
                     /locus_tag="VYO12_001540"
     CDS             2413..2931
                     /locus_tag="VYO12_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1588113.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQAGGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2939..3955
                     /locus_tag="VYO12_001541"
     CDS             2939..3955
                     /locus_tag="VYO12_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1588114.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4040..5296
                     /locus_tag="VYO12_001542"
     CDS             4040..5296
                     /locus_tag="VYO12_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1588115.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5351..6517
                     /gene="recA"
                     /locus_tag="VYO12_001543"
     CDS             5351..6517
                     /gene="recA"
                     /locus_tag="VYO12_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1588116.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            6824..8194
                     /locus_tag="VYO12_001544"
     CDS             6824..8194
                     /locus_tag="VYO12_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1588117.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     YIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8570..9526
                     /gene="lytA"
                     /locus_tag="VYO12_001545"
     CDS             8570..9526
                     /gene="lytA"
                     /locus_tag="VYO12_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1588118.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            9904..10065
                     /locus_tag="VYO12_001546"
     CDS             9904..10065
                     /locus_tag="VYO12_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588119.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            10052..10258
                     /locus_tag="VYO12_001547"
     CDS             10052..10258
                     /locus_tag="VYO12_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588120.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            10304..10657
                     /locus_tag="VYO12_001548"
     CDS             10304..10657
                     /locus_tag="VYO12_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588121.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKKCY"
     gene            10791..11216
                     /locus_tag="VYO12_001549"
     CDS             10791..11216
                     /locus_tag="VYO12_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588122.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVKEFSLEYMTTGDILVEAFKA"
BASE COUNT         3629 a         1860 c         2546 g         3492 t
ORIGIN      
        1 agcaaatagg aaggtttatt atctttaatg aatacactcg cagagaaatt cagattgaaa
       61 agaaaagagt tgagactctc ccaacaaact cttgcagaag gaatttgtga acaaagtcag
      121 attagtaaaa ttgagagagg gcatttcatt ccctccgcag accttttgtt caaactctct
      181 caacgacttg aagtaccatt agattatttt tttaatgaac aaattgaaat taaatctaac
      241 ctctctaatt tcaagcaatt gtctgctcga ctattagacg atagaaatta tgacgatttg
      301 gaatatattt atagaataga gattgaacga agtacttttc taacactaga agaccgaact
      361 tatcttgaat ggattaaagc tattattgac ttttatcaat atgacagtaa gtgtgaagca
      421 atttcttcat tggaaaatat attattaaaa gtctcctcaa atactctgat ttatttaaag
      481 gcgttgaata ctctatctaa tttctattcc ttagtgggtc gtgaacaaga atatgaggca
      541 aactactctc atttaataga gttgtatcag acaaaaaatt tggaccatca agagttttta
      601 tttggctaca tcagagttcg ttacaactac gctcactacc tagtatcaaa ggaaaaatat
      661 aatgaagcca tccaagaagc tcttgagacg attgaactct gtaaacaaag acagacaagc
      721 taccaactgg ctcctctact tattcttgta ggaaatgctg gagccaaatt tctagacaga
      781 gaacaagtca aaaattatta tatagaagca aaagagttat gtaagattta taacaatcct
      841 ttaatgctga tgaagataga aaattatttg aaggaattag atactgttta gttaatcttg
      901 acattatagt ttttctgaaa cgttaaataa cctgtaaggc tgatagtgaa atgaatacta
      961 tcagccttat gattatgtga ctattagttc gtctcgctcc gttaggtgcg agacaaaaaa
     1021 aacacccgct agacggattt tcatgatttc aaatttatat tcttcgaaaa tctcttcaaa
     1081 tcacgtcagc tctatctgca atctcaaaac agtgttttga gcaacctgtg gctagctacc
     1141 tagtttgatc tttgattttc attgagtatt agtctctagt gtttgtttag cgtcttaaag
     1201 agacatttga gaagattaga aacagttctc tcttttctcc tagtataatg attggtagga
     1261 agggagagag aagatgaaat caatgagaat cttatttttg ttagctttaa ttcaaatcag
     1321 tttgagtagc tgtttcctat ggaaggaatg catcttgtcc tttaaacaaa gtacagcttt
     1381 tttcatcgga agcatggttt tcgtttcagg aatctgtgct ggagtaaatt atctttatac
     1441 ccgtaagcaa gaagtccata gtgtcctagc cagtaagaag tcggtgaagc ttttttacag
     1501 tatgttactc ttaattaatt tgttaggagc tgttcttgtt ttgtcagata acttgttcat
     1561 caaaaatacg ctgcagcaag aattagttga ctttttattg ccatccttct ttttcctatt
     1621 tgggctagat ttgctgattt ttttaccctt gaaaaaatac gtgcgcgatt ttcttgctat
     1681 gctagacaga aaaaagacag tgttggtgac tattttagca acacttcttt tcttaagaaa
     1741 tccaatgacc attgtctcac ttctgattta tattggactg ggcttgtttt ttgcagccta
     1801 tcttgtccca aattcggtta agaaggaagt ttccttttat ggtcatattt tccgagatct
     1861 tgtattggtc attgttacgc tcattttctt ttagagaaag cctgtttttt atggatgtta
     1921 tgatgataag tttagtagga aaatagacac aaaagaaaag tttttggtat aataagagta
     1981 tgtacacaaa aaatgaagaa gagttgcaag ccttagggga gcgtttgggc catctattag
     2041 caaagaatga tgttttaatc ttaactggag aactgggtgc aggtaaaacg acctttacta
     2101 aaggacttgc aaaaggctta cagatttctc aaatgattaa aagtcccacc tatactatcg
     2161 tgagagagta tgaaggtcga cttccacttt atcacctaga tgtttatcgt attgaaggag
     2221 atgctgattc tatcgacttg gatgagttta tctttggtgg cggcgtgact gttattgagt
     2281 ggggaaatct cttaggagat gccttgccag atgcttattt agaattggaa attctaaaag
     2341 aagcagatgg acgccgttta aattttcagg caaagggttt gcgtgctgag aaattgttag
     2401 aggagcttca atatggagta tgaattgctc attagggaag cagagcccaa agatgcagct
     2461 gaattagtgg cctttttaaa tcgtgtgagt ttggagacag actttaccag cctagacgga
     2521 gatggtattc tcttgactag tgaggagatg gaaatattcc tcaacaagca agctagttcg
     2581 gacaatcaga taaccttact tgcattctta aatggcaaaa ttgctggtat tgtaaatatt
     2641 acagctgatc agcgtaagag agtccgtcat attggagatc tcttcattgt gattggaaaa
     2701 agatattgga ataatggctt gggaagtttg ttgctagaag aagcgataga gtgggcacaa
     2761 gcaggtggca ttctgcgtcg tctccaactg actgtccaaa ctcgtaatca agcagcagtc
     2821 catctttatc aaaagcatgg ctttgtcatt gaaggtagcc aagagcgtgg ggcatatata
     2881 gaagaaggga aatttatcga tgtttacttg atgggtaaac tgataggtta gtagaaatat
     2941 ggttaaaaaa attattggaa tggtgctagc tttactttct gtaactgtag taggagtagg
     3001 tgtttttgct tatactattt atcaacaagg gacagaaacc ttagctaaaa cctataaaaa
     3061 aatcggcgaa gaaaccaagg ttattgaagc gactgaacct ctaaccattc tgttaatggg
     3121 agtggacacc ggaaatgctg aacgaactga aacttgggtc ggtagaagtg atagcatgat
     3181 cttgatgaca gtgaatccta aaacgaaaaa aacaacaatg atgagtttag agcgggatat
     3241 tctgacgcgc attgaatcag ggaatggtca ggctcatgaa gcgaaactga actcagcata
     3301 tgcagatggt ggagcagagc ttgctataga aaccattcaa aaaatgatga atatccatat
     3361 tgatcgctat gtaatggtca atatgagagg gttgcaaaaa ctagtggatg cagtaggagg
     3421 tattacagtc aataatatcc taggtttccc aatttctatc agtgaccaag aagaatttaa
     3481 tactatttct atcggtgttg gggagcaaca tattggggga gaagaagccc tagtctatgc
     3541 acgaatgcgt taccaagatc ctgaggggga ttatggtcgt caaaaacgtc aacgtgaagt
     3601 tattcaaaaa gtcatggaaa aagctctcag tttaaatagc gttggtcatt atcaagagat
     3661 tctaaaagct ttgagtgaca atatgcagac caatattgat ttgtctgcaa aaagtatccc
     3721 taacttgcta ggctataaag attcatttaa aaccattgaa actcagcagt tgcagggtga
     3781 aggagagata cttcaaggtg tttcttacca gattgtttcg agagcacata tgttggaaat
     3841 gcaaaatata ctccgacgtt ctttgggaca agaagaagtt actcagcttg aaaccaatgc
     3901 ggttttattt gaagatttat ttggcagagc acctgttggt gatgaagata attaactttt
     3961 gatacaaata aaaaaatcaa tcgtaggaaa attcctgcga ttttttcaaa aaaatacgaa
     4021 tagataggta ggaggaaaca tgaaagcaga aatcattgct gttggaacag agattttgac
     4081 aggacagatt gtcaacacca atgcccagtt tttgtcggaa aaactagctg agattggggt
     4141 agatgtatat tttcagacgg ctgtaggaga caatgaagtt cgtctcttgt ctttgcttga
     4201 gattgccagt caacgtagca gtctggtgat tttgacaggc ggtttggggc caactgagga
     4261 cgacctaacc aaacaaaccc tagctaaatt tttagggaaa gcattagtct ttgatcctca
     4321 ggctcaggag aagttggata tcttttttgc cctgcgacca gactatgccc gaacaccgaa
     4381 taacgaaaga caagctcaaa ttgtagaagg agcgattcca ctgccaaacg aaacaggact
     4441 ggctgttgga ggaaaattag aagtagacgg agtgacctat gtcgtccttc caggtccgcc
     4501 aagtgaattg aaacccatgg tcttaaacca acttctaccc aagttgatga cagggagcaa
     4561 gctgtattcc cgagttcttc gtttctttgg gattggcgag agccagttgg ttacgatttt
     4621 ggctgattta attgataatc agatcgatcc taccttggcc ccttatgcca agacaggaga
     4681 agtcactcta cgtctgtcaa caaaggctag cagtcaagaa gaggcgaatc aagcgctgga
     4741 tatcttggaa aatcaaatct tggactgcca gactttcgaa ggaatttctt tacgagactt
     4801 ttgctatggt tatggggaag aaactagttt agccagcatt gtggtagaag aactgaaaag
     4861 gcaagggaaa accatcgcgg ctgcagagag tttgacggca ggtcttttcc aagctaccgt
     4921 ggcgaatttt tctggagttt caagtatatt taagggtggt tttgtgacct atagcttgga
     4981 ggaaaaatca aggatgttgg atattcctgc caagaatttg gaagaacatg gtgtggtgtc
     5041 tgaatttaca gctcagaaga tggctgagca ggcacgaagc aagacccagt ctgattttgg
     5101 aattagtttg accggagtgg caggaccaga tagcctagaa ggacacccag ttgggacagt
     5161 cttcataggc ttggcgcaag agcaaggaac tgaggttatc aaggttaata ttggaggcag
     5221 aagccgagca gatgtacgtc atattgcggt tatgcatgcc tttaacctag ttcgcaaggc
     5281 tttattaagt gactaacttt tgatataata gtagataggt ctgaggatca ttagaatgta
     5341 ggagaataga atggcgaaaa aaccaaaaaa attagaagaa atttcaaaaa aatttggggc
     5401 agaacgtgaa aaggccttga atgacgctct taaattgatt gagaaagact ttggtaaagg
     5461 atcaatcatg cgtttgggtg aacgtgcgga gcaaaaggtg caagtgatga gctcaggttc
     5521 tttagctctt gacattgccc ttggctcagg tggttatcct aagggacgta tcatcgaaat
     5581 ctatggccca gagtcatctg gtaagacaac ggttgccctt catgcagttg cacaagcgca
     5641 aaaagaaggt gggattgctg cctttatcga tgcggaacat gcccttgatc cagcttatgc
     5701 tgcggccctt ggtgtcaata ttgacgaatt gctcttgtct caaccagact caggagagca
     5761 aggtcttgag attgcgggaa aattgattga ctcaggtgca gttgatcttg tcgtagtcga
     5821 ctcagttgct gcccttgttc ctcgtgcgga aattgatgga gatatcggag atagccatgt
     5881 tggtttgcag gctcgtatga tgagccaggc catgcgtaaa cttggcgcct ctatcaataa
     5941 aaccaaaaca attgccattt ttatcaacca attgcgtgaa aaagttggag tgatgtttgg
     6001 aaatccagaa acaacaccgg gcggacgtgc tttgaaattc tatgcttcag tccgcttgga
     6061 tgttcgtggt aatacacaaa ttaagggaac tggtgaccaa aaagaaacca atgtcggtaa
     6121 agaaactaag attaaggttg taaaaaataa ggtagctcca ccgtttaagg aagccgtagt
     6181 tgaaattatg tacggagaag gaatttctaa gactggtgag cttttgaaga ttgcaagcga
     6241 tttggatatt atcaaaaaag caggggcttg gtattcttac aaagatgaaa aaattgggca
     6301 aggttctgag aatgctaaga aatacttggc agagcaccca gaaatctttg atgaaattga
     6361 taagcaagtc cgttctaaat ttggcttgat tgatggagaa gaagtttcag aacaagatac
     6421 tgaaaacaaa aaagatgagc caaagaaaga agaagcagtg aatgaagaag ttccgcttga
     6481 cttaggcgat gaacttgaaa tcgaaattga agaataagct gttaaagcag tggagaaatc
     6541 cgctactttt tcgatttttg attcaagttt ttagattata tatagtagat tgaaataaga
     6601 tatgaacaac tttattagga aagtcaaatt aatttctaga aatgttttag cagctacagc
     6661 gtactattcc aaactcaacc aactataata gatcgaaact agaatagtac acatctactt
     6721 ctaaaacatt gttaaaaatc gatttgactg tccttatttc attccgctat atataagttt
     6781 gctgtttctt gtcgctcctc tggaaagctg atataatagc tttatgaata aaaaacgaac
     6841 agtggacctg atacatggtc cgattcttcc ctcgctctta agcttcacct ttccaatctt
     6901 gttatcaaat atttttcaac agctctataa cactgctgat gtcttgattg ttggacgatt
     6961 tcttggtcaa gaatccttgg ctgcagtagg agcgacgaca gcgatttttg atctgattgt
     7021 aggttttaca cttggtgttg gcaatggcat ggggattgtc attgctcgtt attatggggc
     7081 tcggaatttc actaaaatca aggaagcagt agcagccacc tggattttag gtgctctttt
     7141 gagcattcta gttatgttgc tgggctttct tggcttgtat cctctcttgc aatacttaga
     7201 tactcctgca gaaattcttc ctcaatctta tcaatatatt tctatgattg tgacctgtgt
     7261 aggtgtcagc tttgcttata atctttttgc aggcttgttg cggtatattg gtgacagtct
     7321 agcagccctg ggatttctga ttttctctgc cttggttaat gtggttctgg atctctattt
     7381 tattacgcaa ttgcatctgg gagttcaatc cgcaggactt gctaccatta tttcgcaagg
     7441 tttatcagcg gttctctgct tttattatat tcgtaaaagt gtgccagaac tcttgccaca
     7501 gtttaaacat ttcaaatggg acaaaagctt gtacgcggat ctcttggagc aaggtttggc
     7561 tatgggcttg atgagttcaa ttgtatctat cggcagtgtg attttacagt tttctgttaa
     7621 tacatttggt gcagtgatta ttagtgccca gacggcagct cgacgcatta tgacctttgc
     7681 ccttcttcct atgaccgcta tttctgcatc aatgacgacc tttgcttctc agaatctagg
     7741 agctaagcga cctgaccgca ttgttcaagg tcttcgaatc ggcagtcgtt taagtatatc
     7801 ctgggcagtt tttgtttgta ttttcctctt ttttgccagt ccagccttgg tttccttctt
     7861 ggctagttcg acagatggtt acttgataga aaatggaagt ctctatctgc aaatcagttc
     7921 agccttttat cccattttga gcctcttgtt gatttatcgc aattgcttgc agggcttggg
     7981 gcagaagatc cttcctctag tttctagctt tattgaacta atcggaaaaa tcgtttttgt
     8041 ggttttgatt attccttggg caggatataa gggtgttatc ctttgtgaac ctcttatctg
     8101 ggttgccatg acagttcaac tgtacttctc actattccgt catcccttga taaaagaagg
     8161 aaaggcaatt ttggcaacca aagtgcaatc ctagttggat ttactgaata aaatccattt
     8221 cctctagtga acatcgaaaa aacttgtgtt ctcttcttca gtttggtgtt gaaaatagtt
     8281 taacagactt ttgacttctt ttatatgata taataaagta tagtatttat gaaaaggaca
     8341 tatagagact gtaaaaatat acttttgaaa agctttttag tctggggtgt tattgtagat
     8401 agaatgcaga ccttgtcagt cctatttaca gtgtcaaaat agtgcgtttt gaggttctat
     8461 ctacaagcct aatcgtgact aagattgtct tctttgtaag gtagaaataa aggagtttct
     8521 ggttctggat tgtaaaaaat gagttgtttt aattgataag gagtagaata tggaaattaa
     8581 tgtgagtaaa ttaagaacag atttgcctca agtcggcgtg caaccatata ggcaagtaca
     8641 cgcacactca actgggaatc cgcattcaac cgtacagaat gaagcggatt atcactggcg
     8701 gaaagaccca gaattaggtt ttttctcgca cattgttggg aacggttgca tcatgcaggt
     8761 aggacctgtt gataatggtg cctgggacgt tgggggcggt tggaatgctg agacctatgc
     8821 agcggttgaa ctgattgaaa gccattcaac taaagaagag ttcatgacgg actaccgtct
     8881 ttatatcgaa ctcttacgca atctagcaga tgaagcaggt ttgccgaaaa cgcttgatac
     8941 agggagttta gctggaatta aaacgcacga gtattgcacg aataaccaac caaacaacca
     9001 ctcagaccat gtggatccat acccttactt ggcaaaatgg ggcattagcc gtgagcagtt
     9061 taagtatgat attgagaacg gcttgacgat tgaaacaggc tggcagaaga atgacactgg
     9121 ctactggtac gtacattcag acggctctta tccaaaagac aagtttgaga aaatcaatgg
     9181 cacttggtac tactttgaca gttcaggcta tatgcttgca gaccgctgga ggaagcacac
     9241 agacggcaac tggtactggt tcgacaactc aggtgaaatg gctacaggct ggaagaaaat
     9301 cgctgataag tggtactatt tcaacgaaga aggtgccatg aagacaggct gggtcaagta
     9361 caaggacact tggtactact tagacgctaa agaaggcgcc atggtatcaa acgcctttat
     9421 ccagtcagcg gacggaacag gctggtacta cctcaaacca gacggaacac tggcagacaa
     9481 gccagaattc acagtagagc cagatggctt gattacagta aaataataat ggaatgtctt
     9541 tcaaatcaga acagcgcata ttattaggtc ttgaaaaagc ttaatagtat gcgttttctt
     9601 gtggagatat ttccttcaat ttttgctact atattaaaca aaaatcaaaa agcaaactag
     9661 aaagttatgc tcaaataaaa tctaaatttg acaatgtaaa ccgagtcgga tagctttaag
     9721 tactgttttg aggttgaaga tacgattttt gataggaact catcaaatag gtttaatatc
     9781 atcaatttta gatttttaag tagcatcaat aaattgcttc cttgttttgt cataattttt
     9841 ttatttaaaa aattatgaca agagtgtgct attcttttta tgagaggtgt atgaatatga
     9901 taaatgtatg tgataaatgt atgtgatgtt ggaaaaagaa taaaagaact tagaatatct
     9961 tcaaatctta ctcaagataa gattgctgag tatttgtctt tgaatcaaag catgattgcc
    10021 aaaatggaaa aaggtgaaag gaatatcacg aatggattta agtaataaag ctccaaatct
    10081 tagaaaaaag ttgggagctg atggtgaatc gccgatagat atttttaaat tggtacaaaa
    10141 gatagaaaat ttgacgctgg tattttatgg actcggaaag aatattagcg gagtctgtta
    10201 taaaggaact cagttcagtc tcattgcagt caattcagac atgtcattag gaaggtaaag
    10261 attttcttta gcacatgaac tgtatcatct ttattatgat gaggtgaaga agagttcagt
    10321 cagtcttatc ttgattggtg aaggagatga aactgaaaga aaagcggatc agtttgcttc
    10381 ttatttttta attttcccat cttcactgta taggatggtt gaggaaatca gagaaaatgc
    10441 caatagaact catcttgaag tagaagatat tataaaattg ggtcagtttt atggtatcag
    10501 tcataaagct atgttatata gattgaggaa tgatggatac cttgatgcag aagaaattaa
    10561 aaatatggat attagtgtta tagagacagc ttcaagatta ggctatgata caagtttata
    10621 tcgtcctttg tcagaaagta aaaagaaatg ttattaggat aatatattaa tctaactgaa
    10681 caacttttag aaaataacag aatttcgcaa gggaagtatg aggaactgtt actagatgct
    10741 ttcagatatg atattgtata tgggctagat gaagaggggg gagttgtcgt ttgactagtc
    10801 gtgtatttat tgatgcagat tgtatttcag tatttttatg ggttggcact gaacatcttt
    10861 tagaaaagct ctatttgggt aaaattgtta ttccacaaga ggtgtatgat gaaatcaata
    10921 tacctacaat tccccattta aaatctagga tagatcagtt ggtagctaag ggttcagctg
    10981 agattgtgag catagacatt ggaactgaag aatacgcatt atatagagat ttaacaagaa
    11041 atcatgatag taacaagatt attggtaagg gagaagcggc ttctatttcc ttagcgaaaa
    11101 agcataatgg gatattagga agtaataacc taagagatgt taaatcatat gtaaaagaat
    11161 tttctttaga atatatgaca acaggagata tactggttga agcgtttaaa gcgtaattta
    11221 ttactgaaca agagggcaat catatctgga ataatatgct taaaaagaga aggaaaattg
    11281 gtgcaaattc attttcagac tatcttcgtg gaagtattca tcaaaataga caaaaataaa
    11341 tttggataaa tcgaactcac tattcaggag gcatatgagc aattcgaaaa agaaaagtgt
    11401 caaattgagc ctataggagt agaagtgaaa tagtaagtcc tgcatagtgg atgagagaaa
    11461 agttctcctt gaagttttcc tgaactatca gtcgcatgtc aaacgatatg tagggtaatg
    11521 tgagagg
//
LOCUS       DAVLXF010000063        11516 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLXF010000063 DAVLXF010000000
VERSION     DAVLXF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.063"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            103..756
                     /locus_tag="VYO12_001550"
     CDS             103..756
                     /locus_tag="VYO12_001550"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1588123.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            766..1230
                     /locus_tag="VYO12_001551"
     CDS             766..1230
                     /locus_tag="VYO12_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1588124.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1243..2511
                     /locus_tag="VYO12_001552"
     CDS             1243..2511
                     /locus_tag="VYO12_001552"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1588125.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALFLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2525..2785
                     /locus_tag="VYO12_001553"
     CDS             2525..2785
                     /locus_tag="VYO12_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588126.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2796..4157
                     /gene="pdrM"
                     /locus_tag="VYO12_001554"
     CDS             2796..4157
                     /gene="pdrM"
                     /locus_tag="VYO12_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1588127.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4311..5279
                     /locus_tag="VYO12_001555"
     CDS             4311..5279
                     /locus_tag="VYO12_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1588128.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5295..6287
                     /locus_tag="VYO12_001556"
     CDS             5295..6287
                     /locus_tag="VYO12_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1588129.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6505..7548
                     /locus_tag="VYO12_001557"
     CDS             6505..7548
                     /locus_tag="VYO12_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1588130.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     RVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7612..9297
                     /gene="lpdA"
                     /locus_tag="VYO12_001558"
     CDS             7612..9297
                     /gene="lpdA"
                     /locus_tag="VYO12_001558"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1588131.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELES
                     EEDGYLIAILKGEGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESSLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVITDKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9361..10350
                     /locus_tag="VYO12_001559"
     CDS             9361..10350
                     /locus_tag="VYO12_001559"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1588132.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10423..11493)
                     /gene="xerS"
                     /locus_tag="VYO12_001560"
     CDS             complement(10423..11493)
                     /gene="xerS"
                     /locus_tag="VYO12_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1588133.1"
                     /translation="MKREILLERINKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAINLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3385 a         2162 c         2615 g         3354 t
ORIGIN      
        1 gtatatatag tcgatttatt aaatctttag ataaactgta aaaagtggtt tccatagcca
       61 ctttttgcta taatagaggc agtaaaaatg aaggagtcgg ctatggaaca ctcgtcttgg
      121 catgctttga ttaaggcgca attacctgag ggttatttcg ggaaaatcaa tcagtttatg
      181 gagcaggtct attctcaggg gattatttat ccacccaagg aaaaggtttt tcaggctctc
      241 ttgacaacac tgcttgaaga agttaaggtg gtaattctag ggcaagaccc ctatcacgga
      301 ccaggtcaag cgcagggctt gagtttttct gtacctgact ctatcccagc tccaccatcc
      361 ttgcaaaata tcttgaaaga attgtcagat gatatcggag ttaagaaatc tcatgatttg
      421 acagcttggg ctgagcaagg agtcttgctt cttaatgctt gtttgactgt tcctgctgga
      481 caggccaatg gtcatgctgg tcaaatctgg gagcctttta ctgatgctgt gattcaggtg
      541 gtcaatcatc tagatagacc agtcgttttt gtactctggg gagcttatgc acgtaagaag
      601 aaggcattag ttaccaatcc tcatcacttg attatcgaat cagcccatcc aagtcctttg
      661 tcggtttata gaggattttg gggttccaag cccttttcca aggccaatgc attcttaaaa
      721 gagacaggac aagagccaat cgattggctt agataaggag aaaatatgcc tcagttagcg
      781 acgatttgct acattgataa tgggaaagaa ctgctcatgc tccatcgtaa taagaaaccc
      841 aatgatgtcc atgaagggaa atggattggt gtgggtggta agctagagag aggagagacg
      901 ccccaggaat gcgcggcgcg tgaaatcctt gaagaaacag ggctcaaagc caagccagtt
      961 ctaaaaggtg tcatcacttt tcctgaattt acaccagatt tagactggta cacctatgtt
     1021 tttaaggtga cggagtttga gggcgacttg attgactgca atgaggggac gctagaatgg
     1081 gttccctatg atgaggtttt gagcaagccg acttgggaag gtgaccacac ctttgttgag
     1141 tggcttttag aggataaacc cttcttttca gccaagtttg tttatgatgg ggataaattg
     1201 ttggataccc aagttgattt ctatgaataa aggagaaagc agatgctact aatcaaaaat
     1261 ggtcgtgtaa tggatcccaa gtctggtttg gatcaagttt gtgatgtctt agttcaagat
     1321 gggaaaatta tcaaaattgc gcctgagatc acggaagaag gagcagaaac gattgatgct
     1381 actggtcttg tggttgctcc tggtttggtc gatattcatg tgcatttccg tgaacctggt
     1441 caaacacata aagaagacat tcatactggt gccctggcag ccgctgcagg tggttttact
     1501 actgtcgtca tgatggctaa taccagtcca accatttcag acgtggagac tttgcaagca
     1561 gttctccagt cagctgccaa agagaagatt aatgtcaaga cagttgcgac cattactaaa
     1621 aactttaatg gtaaaaactt gactgacttt aaggcgctct tagaagctgg tgcggctggt
     1681 ttctctgatg acggtattcc gcttgagagc agtaagattg tcaaggaagc catggaggaa
     1741 gccaaaaaac tcaatacctt tatcagcctt catgaggaag atccaggttt gaacggtgtt
     1801 cttggcttta atgaaaatat tgctagagaa catttccata tctgcggtgc tactggggtg
     1861 gctgagtacg ctatgatggc gcgtgatgtc atgattgcct atgcaactaa agcccatgtt
     1921 cacatccagc atttgtctaa ggaagaaagt gttaaagtag tggagtttgc tcaggggtta
     1981 ggtgcagaag tcacagcaga agtagcgcca cagcatttct ctaagacaga agcacttttt
     2041 ttaacacaag gtagcaatgc taagatgaat ccaccgcttc gtttggaatc agaccgtcgt
     2101 gccgttatcg aaggtctcaa atcaggtgtc atcacagtta ttgcgactga ccacgcgcct
     2161 catcatgtag atgaaaaaaa tgttgaggat attaccaaag cgccatctgg tatgactggc
     2221 ttagaaacat ccctgtctct cagcttgacc tatttagtag aagctggtga gttgagcttg
     2281 atggaattac ttgaaaaaat gacatacaac ccagccaagc tttacaactt tgaagcaggt
     2341 tacttggctg agaatggtcc agcagatatc actatttttg atgccaaggc tgaccgcctt
     2401 gtggactccc attttgcttc caaagcagct aattcaccat tcatcggtga aaccttaaaa
     2461 gggcaggtta aatataccat ctgtaaggga caaatcgtct atcaagcttg ataggagtta
     2521 gaatatgaat tattttcgaa aacgtaggga gagacaagcg aaatctaata gtggaattta
     2581 ctgtcccgca gctaatcgtt ccaaaattca atatgaaact aaagaaaaag ctgaccatgc
     2641 ggttcaatat gataacggag gtttagttag aagctattat tgtcgtacat gtgcgtgttg
     2701 gcatactact tcaaaatcta ataaacctcc gctaagtttg aaaaactatg gtctgaaact
     2761 ctttggttta gataaatcag aatagaaaga gaagtatgta taagacaaag tgtttacgag
     2821 agaagttagt attattttta aaaattttct tcccaatcct gatttaccaa tttgccaatt
     2881 attctgcctc ttttgttgat actgcaatga caggtcaata caacactatg gacttggctg
     2941 gtgtatctat ggcaaccagt atctggaatc ctttctttac atttctaaca gggattgtgt
     3001 cagccttggt gcctatcatt ggtcaccatc ttggtcgagg aaaaaaggaa gaagttgcgt
     3061 ctgattttta ccaatttatt tatttggcct tgggcctatc tgtggtattg ctggggatgg
     3121 tacttttctt ggcaccaaca atcttgaatc atattgggtt agaagcagca gtagcggcag
     3181 tagcggttcg ctatctttgg tttttatcta tcgggattat ccccttgttg ctctttagcg
     3241 tcattcgttc cttgctggat tcgctgggct tgaccaaact gtccatgtac ctcatgcttt
     3301 tgttactccc tctcaatagc ggatttaact atctcttgat ttacggtgcc tttggtgttc
     3361 cagaactggg aggggctggt gctggtttag gaacatcctt ggcctactgg gtcttgcttg
     3421 ggatttctgt tctggtttta tttaaacagg agaagctcaa agccttacac cttgagaaac
     3481 gaattccact taatatggat aaaattaagg aaggagttcg tttaggtctg cctattgggg
     3541 gaactgtctt cgcggaagtg gctatctttt cagtggttgg cttgattatg gctaagtttt
     3601 cgtccttgat tatagctagt caccagtcag ctatgaactt ttcaagtctt atgtacgcct
     3661 ttcctatgag tatctcatcg gctatggcta ttgtcgtttc ctatgaagtg ggagccaagc
     3721 gatttgatga tgcgaaaacc tatattggtc taggaagatg gactgccctc atttttgcgg
     3781 ccttcacctt aaccttcctt tacattttta ggggaaatgt ggccagtctt tatggtaacg
     3841 acccaaaatt tatcgatttg acagtgcgtt ttttaactta tagtcttttc ttccagttag
     3901 cagatacctt tgcggcgccg cttcagggaa ttttgcgggg gtataaggat acagttattc
     3961 ctttttacct tggtttgctt ggttattggg gcgtagcaat ccctgtggct acgctatttg
     4021 attctctaac agattttgga gcttattctt actggatcgg cttgattatt agtttgattg
     4081 tgagtggggc gctctaccgt tggcgtttaa ctgtgattat gaagagattt gaatctttag
     4141 caaaatccaa acgctaaaaa gtttgtgaaa aaatattctt gataagaaaa tcccttgttt
     4201 tcacggggtt ttctttttta ttttgtgaaa tttcttttag aaaaactttg acagtatttg
     4261 ccaaaaacgg taaaatagta aggtaagaag aaagttagaa aggcaagaag atgtcaactt
     4321 tagataaaaa tcttttgcta gaaatgttcc gtaagatgga agaaatccgt cgcatggact
     4381 taaaaattgc acaattagta aagaaaggga aagtgccagg aatgacgcac ttttctgttg
     4441 gtgaagaggc agctaacgtg ggggctatgt tagctctcaa tccagatgat ctgattacct
     4501 caaaccaccg tggacacggg caagctattg ctaaagggat tgacctcaac ggaatgatgg
     4561 ctgaaatcct tggtaaatac actggaacct gtaaagggaa aggtggatct atgcatatcg
     4621 ctgaccttga tgctgggaac cttggtgcca atggtattgt aggtggtggt atggggatcg
     4681 ctgtcggtgc agccctcagt cagcaaatgc aaaataccgg taaaatcgtt gtctgcttct
     4741 ttggagatgg tgcgaccaat gaaggtgttt tccacgaagc agtgaacatg gcttctatct
     4801 ggaacctgcc agtcattttc tattgcatta acaacggtta cggtatctct gcggatatca
     4861 agaaaatgac caatatagaa catatccatc aacgtagcgc cgcttatgga attcctggaa
     4921 tgttcatcga agatggtaac aatgtcatcg atgtctatga aggatttcag aaagctgtgg
     4981 accatgttcg cagtggcaat ggtccagtct tgattgaaag tgtaacttat cgctggctcg
     5041 gtcactcatc atctgaccct ggtaaatatc gtacgcgtga agaagtggaa ttgtggaaac
     5101 aaaaagatcc aatcgaaaac ctccgcaatt accttattga aaataacatt gcaagtgccg
     5161 aagaattgga agaaatccaa gcgcaagtaa aggaagcagt agaagcttct gttaaatttg
     5221 cagaggaaag cccattccca ccgcttgaat cagcctttga agatatttac gcagactaag
     5281 gagaaagaga taaaatggaa acaaaaacaa tgtccttccg tgacaccatt atccttgcta
     5341 tgtctgagga aatgcgtcgc gatgaaaatg tgttcttgat gggagaagac gtcggtgtct
     5401 tcggaggaga cttcggtact tctgttggaa tgcttgaaga atttggtcca gaacgcgtcc
     5461 gtgactgtcc gatttctgaa gctgccatct ctggagcagc agcaggagct gccatgacag
     5521 gacttcgtcc aatcgtcgat atgaccttca tggacttctc tgttattgcc atggacaata
     5581 tcgtcaacca agctgctaaa acacgttaca tgtttggtgg taaaggtcag gttccaatga
     5641 ctgttcgttg tgcagctggt aacggagttg gctctgccgc ccagcactca caatctctag
     5701 agtcttggtt tactcacatt ccaggactta aggttgtggc acctggaaca cctgcggaca
     5761 tgaaaggcct gcttaagtct tctatccgtg ataaaaaccc agttattata cttgagtaca
     5821 agtcagaatt taaccaaaaa ggggaagtgc cagttgatcc agactacaca attccacttg
     5881 gggttggtga aatcaaacgc caaggaacgg atgtaacagt tgttacttat ggaaaaatgc
     5941 ttcgccgtgt ggttcaagct gctgaagaat tagcagaaga aggaatttca gttgaaattg
     6001 ttgacccacg tacccttgtt ccgcttgata aggatatcat cataaactca gtgaagaaga
     6061 ctggtaaggt tgttctggtc aacgatgccc acaaaacaag tggctatatc ggtgaaattt
     6121 cagctattat ttcagaatca gaagcatttg actatctaga tgcaccaatt cgccgttgtg
     6181 caggagaaga tgtgccgatg ccttatgcac aaaacctaga aaatgcaatg attccaacag
     6241 ttgaaagtat caaagatgca atccgaaaaa cttataacaa agaatagagt tacataagat
     6301 aagttatgta aataaaataa agctgacgag aaactttgta tcttttaggt gcagagctct
     6361 tcgtcattaa tatcttagat tagagcacgg gctaaaagct cggaaaaaag ataaatctcc
     6421 ttggcttcat cgaagccatc gtcgatttcc tatttttcag tcgcttttga cgccctttgt
     6481 atcataaata gaattggaga ggtcatggct gatgacaagc taagagcgac tcctgcagct
     6541 agaaagttag cggatgattt agggataaac ctctacgacg tttctggctc aggtgcaaac
     6601 ggtcgtgtcc acaaagaaga cgtggaaact tataaagaca caaacgtggt tcgcatttcg
     6661 ccacttgcaa aacgaattgc cctcgaacat aacattgctt ggcaggaaat ccaaggaacc
     6721 ggtcatcgtg gtaaaatcat gaagaaggat gttttggccc tgcttcctga aaatatcgaa
     6781 aacgatagca tcaaatctcc tgctcagatt gaaaaagtgg aagaagtccc tgataacgta
     6841 acaccatatg gtgaaatcga gcgtattcca atgacaccaa tgcgtaaggt tattgcccaa
     6901 cgcatggttg aatcttactt gactgcgcca accttcaccc tcaactatga agttgatatg
     6961 actgaaatgt tggctcttcg taagagggtt cttgagccaa tcatggaagc aactgggaag
     7021 aagactactg taacagacct tctttcactt gcagttgtta agactcttat gaaacatcca
     7081 tacatcaacg cttcattgac agaagatggc aagactatta tcactcacaa ctatgtcaat
     7141 cttgccatgg cagttgggat ggataatgga ttgatgacac ctgttgttta caatgctgag
     7201 aagatgagtc tttcagaact ggttgtagcc tttaaggatg ttattggccg taccttggat
     7261 ggtaaattgg ctccaagtga gttacaaaat tcaacattca caatcagtaa tttgggaatg
     7321 tttggtgttc agtcctttgg tccgattatt aaccaaccca actcagctat ccttggtgtc
     7381 agttcgacaa tcgagaagcc agttgtcgtc aatggtgaaa ttgtgattcg cccaatcatg
     7441 agtttaggat taactattga ccaccgtgtc gtagatggta tggctggtgc taagtttatg
     7501 aaggacttga aagagttgat tgaaacccca atctcaatgt tgatttaaga gcaagagtat
     7561 tcatttttaa gatatagata aaggaaggaa gacatggcct tagaagtaat tatgccaaaa
     7621 gccggcgtgg atatgacaga aggacaaatc gtccaatgga ataaaaaagt cggagaattt
     7681 gtaaaagaag gagaaatcct tttggaaatc atgactgata aagtcagcat ggaattggaa
     7741 tccgaagaag atgggtactt gattgccatt ctcaaaggag agggtgaaac tgtccctgta
     7801 acggaagtta tcggttacct tggtgaagaa agggaaaaca tcccaacagc tggagcagca
     7861 tcgccagaag ctagccctgt acctgtagca agtacctcaa acgatgatgg taagagcgat
     7921 gatgcttttg atatcgttgt gattggtgga ggtcctgctg gttatgttgc agccattaaa
     7981 gctgcccaac tcggcggtaa ggttgccctt gttgagaaat ctgaacttgg tggaacctgc
     8041 ttaaaccgtg gatgtattcc aaccaagacc taccttcata acgctgaaat tattgaaaat
     8101 atcggtcatg ctgcaaatcg tggtatcgtg attgaaaatc ctaacttcac tgttgatatg
     8161 gaaaaacttt tagaaactaa atctaaagtt gttaatactc ttgttggtgg tgttgcagga
     8221 cttcttcgta gctacggagt tactgttcat aaaggaattg gtacaatcac taaagacaag
     8281 aacgtcttgg taaatggttc tgaattgctt gaaaccaaga aaatcattct tgctggtggt
     8341 tcaaaagtca gcaagatcaa cgtccctggt atggaatctt cacttgtcat gactagtgat
     8401 gacattcttg aaatgaacga agtgccagaa agccttgtta tcatcggtgg tggagttgtc
     8461 ggtatcgaac tcggtcaggc cttcatgaca tttggttcaa aagtgactgt tatcgaaatg
     8521 atggaccgta tagttccagc tatggatgcg gaagtttcta agaaccttcg cttgatcctt
     8581 gagcgtaaag gaatgaccat cttgactggt actaaactgc aagaaatcat tgaggaaaat
     8641 ggtcaacttc gtatcaaggt tgaaggaaaa gacgatatca tcgcaagcaa agctcttctt
     8701 tcaattggtc gtatgccaga ccttgaaggt attggagagg ttgagtttga attggatcgt
     8761 ggttgtatca aggtcaacga atacatggaa acttcagttc caggcattta tgcaccaggt
     8821 gacatcaacg gtactaagat gttggctcac gcagctttcc gcatgggtga agtttccgct
     8881 gaaaatgccc ttaaaggaaa tcatgcagtt gccaaattga atttgactcc tgcagccatc
     8941 tacactctcc ctgaagtagc agcagtaggt ttgacagaag aacaagcccg tgagaaatac
     9001 gatgttgcca tcggtaagtt taactttgct gctaacggtc gtgctattgc atctgacgct
     9061 gctcaaggtt tcgtaaaagt tatcactgat aagaaatacg gagaaatcct tggtgtacac
     9121 attattggtc ctgcagccgc agaattaata aacgaagcat caagcatcat cgaaatggaa
     9181 atcactgttg aggaaatgct gaagaccatc cacgggcacc caacttactc tgaagtgatg
     9241 tacgaagcgt ttgcagatgt tctaggaatg gccatccatt cacctaagaa aaaataaaac
     9301 aaagatagat tagactggaa ggttatttcc agtttctatc atataaagta aagggatatc
     9361 atgaaataca ttatcaatca ttcaaatgac actgctttta atattgcctt ggaagaatac
     9421 gcctttaaac accttttgga tgaggatcaa atcttcctac tttggattaa caagccatct
     9481 atcattgttg gtcgtcacca gaacactatc gaagaaatca accgtgatta tgttcgcgaa
     9541 aatggtattg aggtagttcg ccgtattagt ggtggtggag ctgtttacca cgatttaaat
     9601 aacctcaact acacgatcat ctccaaagaa gatgaaaata aggcatttga cttcaagagc
     9661 ttctcaactc cggttatcaa taccctggct caacttgggg ttaaagctga gttcacaggt
     9721 cgtaatgacc ttgagattga tggtaagaag ttctgtggca atgcccaagc ctatatcaac
     9781 ggccgtatca tgcaccacgg ttgcttgctc tttgacgttg atttgtcagt cctcgcaaat
     9841 gcccttaagg tttcaaaaga taaatttgaa tcaaaaggtg tgaaatccgt ccgtgcccgt
     9901 gtaaccaata ttatcaatga attaccaaaa aaaatcacag tcgaaaaatt ccgtgattta
     9961 ctcttggaat acatgaaaaa agagtaccca gagatgactg aatacgtctt ttcagaagaa
    10021 gaattggccg aaatcaatcg catcaaggat actaagtttg gaacttggga ctggaactat
    10081 ggtaaatcac ctgaatttaa cgtccgtcgt ggaaccaaat tcactagtgg taaggttgaa
    10141 gtctttgcta acgttactga atcaaaaatc caagacatca agatttatgg tgacttcttt
    10201 ggtattgaag acgttgctgc agtagaagat gtccttcgtg gggtgaaata cgaacgcgaa
    10261 gatgttctta aggcgctaaa aaccattgat atcacacgct acttcgctgg tattagccgt
    10321 gaagaaatcg ctgaagcggt agttggataa atcaaaaaga agttggccaa ctaaccaact
    10381 tctttttttg atatttattt acataattta aaatacgtaa aatcataaac tatccagagc
    10441 attcttttgt tcatcattaa caatatgggt atagaggtca gtgacttgtg tgctggcatg
    10501 tcctagttgg tgactgacta aaacttgtga tttagtagca tcatagagcc tagttgctag
    10561 tgtatggcgc agtttatggg gtgttacacg tactttaaaa tcctctgagt atttagcaac
    10621 cattttctca acgctagaag catcgatacg attaggaaca cctctgtaga gagttaaaaa
    10681 aagggctgta tctgtttttt ccgttttata gcgttgattc cgaatggcca gataattctc
    10741 taaataaggt ttagcaaaag cagcgacatt gactgagtca cgtttgccac cttttcgagt
    10801 aacatcaata accatcattt ttagattgag atctcttaga tctagattaa tagcttcaga
    10861 taagcgaaca ccagatgcca agagaagggc aataatggct aaatctcgtt ctttattttt
    10921 gttgaatgat gagagagctc gatttgaaag ctgttgtggg tgctcttgat cgatataagt
    10981 tagaaaacct tctgtttcat cacctagaaa gagtttttgc ttgatatttt cagctctggc
    11041 agcaagggtt tctttctttt tcttggttga aacttttttc attacattac gatagaaata
    11101 aggttccccc tgatcgtttt caacctcctc ggttagatac ttgtaaagac tagaaagtgc
    11161 tgataaggtt cgattgatag ttgtctgtga aacaccttgt tttgttgtat tagcattcag
    11221 caagggacgt tcacgtagat aaaggataaa ggattccatg tctttcttag acatattttc
    11281 caaaactgat aaaggaatat cggatatttt atcagcgttt gaaataccag actccaaaac
    11341 ccagctgaaa aatcggtcat attccttaag gtattcgtac agggttgtaa aactgtaggg
    11401 cacagccagt ttagattggt agtattccag aacataccag ggcatgagtt gttttagttt
    11461 gttgattcgt tccagtaaaa tctcacgttt catgtatttt ctccataagt aagtat
//
LOCUS       DAVLXF010000064        11040 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLXF010000064 DAVLXF010000000
VERSION     DAVLXF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.064"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            77..364
                     /locus_tag="VYO12_001561"
     CDS             77..364
                     /locus_tag="VYO12_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588134.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNTLKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLG"
     gene            974..1666
                     /locus_tag="VYO12_001562"
     CDS             974..1666
                     /locus_tag="VYO12_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1588135.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1719..4130
                     /locus_tag="VYO12_001563"
     CDS             1719..4130
                     /locus_tag="VYO12_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1588136.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGRI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDEKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4380..5081
                     /locus_tag="VYO12_001564"
     CDS             4380..5081
                     /locus_tag="VYO12_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588137.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5083..7791
                     /locus_tag="VYO12_001565"
     CDS             5083..7791
                     /locus_tag="VYO12_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1588138.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGCFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTIPGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NVFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     YKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7887..8378)
                     /gene="tpx"
                     /locus_tag="VYO12_001566"
     CDS             complement(7887..8378)
                     /gene="tpx"
                     /locus_tag="VYO12_001566"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1588139.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8501..9430)
                     /gene="psaA"
                     /locus_tag="VYO12_001567"
     CDS             complement(8501..9430)
                     /gene="psaA"
                     /locus_tag="VYO12_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1588140.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKATTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9462..10310)
                     /locus_tag="VYO12_001568"
     CDS             complement(9462..10310)
                     /locus_tag="VYO12_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1588141.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10307..11029)
                     /locus_tag="VYO12_001569"
     CDS             complement(10307..11029)
                     /locus_tag="VYO12_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1588142.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3226 a         2051 c         2534 g         3229 t
ORIGIN      
        1 tatcagaaaa tacttttttt aaaaagcaaa tatgatacaa ttttatttga aaaaaaataa
       61 aaaaggagat tttattatga aatcaagtgg tatcgtcggt cttgtgggag gaattttact
      121 tcttatttgt ccttttgtct tgttgggaac agcggtaaat acagctgcta caactcttaa
      181 tggaggagct actgcagggg ctttttcagg tgttgcctta ctcttgaata ccttgaagat
      241 tgcaaatctt gttcttggta tcattgctat tgtttactat aaaggagata agcatgtagg
      301 tgcagctccg tctgtactaa tgattgtttc tggtgaagtt agtctcattc cattcttagg
      361 atgagttggg gggattcttg ctattatcgg aggatctcta ttccttgcaa cattgaagaa
      421 attcaaatca gaagaataaa aggtatttta gcatgaaaag aacaaaaaag tttatcggta
      481 taggagtagc tctattatct ctttctcttc tagttgcatg tggaacataa agttcaaaga
      541 atacttcaac aagtaatgat gagaagacag tagcaacatc caatagttca aaagaaacaa
      601 tcactttcga tacaccggtt gtaacagacg atgcgattga atcaatacgc acttatgcag
      661 attatataga tctttataaa aatatttttg atgattattt tactaaagct gaggaaggtt
      721 tcaaaggcac agttatggaa aataatgact cgtttactaa actaaaagag tcaactcaaa
      781 aattattcga tgcgcagaaa aaaaggttaa ataatgaaga tagaatagaa acagccaaaa
      841 acaatgtgat tgccaaacat tgtcaaacag tcctttcctt tttggttttg actagctttt
      901 ttgtgaaaaa ttgtgtaaaa tagaatagat aaacgagggg aaacctcgga aaatttaaag
      961 gagaatccat ctaatggtaa aattggtttt tgctcgccac ggtgagtctg aatggaacaa
     1021 agctaacctt ttcactggtt gggctgatgt tgatttgtct gaaaaaggta cacaacaagc
     1081 gattgacgct ggtaaattga tcaaagaagc tggtatcgaa tttgaccaag cttacacttc
     1141 agtattgaaa cgtgctatca aaacaactaa cttggctctt gaagcttctg accaattgtg
     1201 ggttccagtt gaaaaatcat ggcgcttgaa cgaacgtcac tacggtggtt tgactggtaa
     1261 aaacaaagct gaagctgctg aacaatttgg tgatgagcaa gttcacatct ggcgtcgttc
     1321 atacgatgta ttgcctccaa atatggaccg tgatgatgag cactcagctc acacagaccg
     1381 tcgttatgct tcacttgacg actcagtaat cccagatgct gaaaacttga aagtgacttt
     1441 ggaacgtgct cttccattct gggaagataa aatcgctcca gctcttaaag atggtaaaaa
     1501 cgtattcgta ggagctcacg gtaactcaat ccgtgccctt gtaaaacaca tcaaaggttt
     1561 gtcagatgat gagatcatgg acgtggaaat ccctaacttc ccaccattgg tattcgaatt
     1621 cgacgaaaaa ttgaacgttg tttctgaata ctaccttgga aaataaaaaa ttgtaagcct
     1681 aggattgatt tctaggcttt ttatgttagt atggaagtat gataaggaat aaaaaacaag
     1741 attatgtact ggcctacaaa caaccagctt caaccactta catgggttgg gaagaagaag
     1801 ctttaccgat aggtaatggt tctttaggag caaaagtatt tggccgtata ggggctgaac
     1861 ggattcaatt taatgaaaaa agtctctggt ctggaggtcc acttcctgat agttcagatt
     1921 atcagggtgg aaatcttcag gatcagtatg tttttttagc tgagattcgg caggctttgg
     1981 agaagagaga ttacaatctg gctaaggaac tggctgagca gcacctaatt gggccaaaaa
     2041 cgagtcaata tgggacctat ctgtcttttg gggatattca cattgagttc agccagcaag
     2101 gtacgacttt gtctcaggtg acggactatc agagacagct gaatattagt aaggcacttg
     2161 tgacgacttc ttatgtctat aagggaacgc gatttgaacg tgaagctttt gcgagttttc
     2221 cagatgatct cttggttcag cgttttacta aggaaggggc ggaaactcta gattttacta
     2281 tagaactatc cttgacctgt gatttggctt ctgatgaaaa gtatgagcag gaaaaatctg
     2341 attacaagga gtgtaagttg gatattactg attctcatat cttgatgaag ggaagagtta
     2401 aggataatga tctgcggttt gctagttatc tagcttggga aacggatgga gatattagag
     2461 tttggtcaga tagggttcag atatcaggag ccagttatgc caatctcttc ttggccgcta
     2521 agacggattt tgcccaaaat cctgctagca attatcgcaa gaaactagat ttagagcaac
     2581 aggtgataga cttggtggac acagctaaag aaaagggcta tacccaattg aaatcaaggc
     2641 atatcgagga ctaccaagcc ttattccagc gtgttcaatt ggatttggaa gctgatgttg
     2701 acgcatccac tacagatgat ttgttaaaaa attataagcc acaagaaggg caggctttgg
     2761 aggagctgtt cttccagtat ggacggtatt tattgattag ttcgtccaga gactgcccag
     2821 atgctctacc agctaaccta cagggagtct ggaatgcggt cgacaatcct ccttggaatt
     2881 cggactatca cttaaatgtc aatctgcaga tgaattattg gccagcctat gttaccaatc
     2941 tcctagaggc ggtctttccg gtcatcaact atgtagatga tttgcgtgtc tatggtcgtc
     3001 tagcggctgt aaagtatgca ggaatcgtct ctcagaaagg tgaggaaaat ggttggttgg
     3061 ttcatactca agcgactccc tttggttgga cggcacctgg ttgggattac tattggggtt
     3121 ggtcaccagc tgccaatgcg tggatgatgc aaaccgttta tgaagcctat tcattttata
     3181 gggaccaaga ctatctcagg gagaaaattt atcccatgtt gagggaaacg gttcgttttt
     3241 ggaatgcctt tttacataag gatcagcagg cgcagcgttg ggtgtcttct ccgtcttatt
     3301 ctccagaaca tgggccgatt tcgattggca atacctatga ccaatctctg atttggcagt
     3361 tatttcatga ttttattcag gctgctcagg aattgggact ggatgaggac ttgttgactg
     3421 aggttaagga gaagtctgat ttactaaatc ctttgcaaat cactcaatct ggtcgaatca
     3481 gggagtggta tgaggaggaa gagcagtatt ttcaaaatga gaaagtggag gcccagcacc
     3541 ggcacgcttc ccatctagtg ggactctatc ctggcaatct ctttagctac aagggacaag
     3601 agtatattga agcggcgcgt gctagcctca atgatcgtgg agatggcggc acaggctggt
     3661 ccaaggctaa taagatcaat ctctgggcgc gtttgggaga tggcaatcga gcccataaat
     3721 tattggcaga gcagttaaag acatccacct tgccaaatct ttggtgtagc catcctcctt
     3781 ttcagataga tggtaatttt ggtgctacta gtggcatggc agaaatgtta ctccagtctc
     3841 atgcagctta tctggtacct ctagctgccc tacctgatgc ttggtcaaca ggttctgttt
     3901 caggcttaat ggcacgtgga cattttgaag tgagcatgag ctgggaagat aaaaaactct
     3961 tacagttgac cattttatca aggagtggag gagatttgcg agtttcttat ccagatattg
     4021 agaagagtgt gattaaaatg aatcaagaaa aaataaaagc gaaatgcatg gggaaagatt
     4081 gtatttcggt ggcaacagca gaaggtgatc ttgttcaatt ttatttttaa gaagatgtta
     4141 taaggcagta atttgaaact gccttttaat aaggatttaa gaatataagc agttttcaac
     4201 tagttgaaaa agcgttataa tgataatagg aagtaatact caatgaaaat caaagagcac
     4261 aaacaaggaa gctagccgca ggttgctcaa aacagtgttt tgaggttgca gatggaagct
     4321 gacgtggttt gaagagagat tttcgaggag tataatttgt ttgatagagg gtgggtctga
     4381 tggcttatat tgagatgaaa cactgttaca agcgttatca ggttggggac acggagattg
     4441 tggccaatcg tgatgtgaat tttgagattg aaaaggggga gctggttatt atccttggtg
     4501 cttcaggtgc aggcaagtca acagtgctta accttcttgg gggaatggat accaatgatg
     4561 aaggggaaat ctggattgat ggtgttaata ttgcggatta tagttcccac cagcgcacca
     4621 attaccgtag aaatgatgtg gggtttgttt ttcagtttta taatctagtt tctaatctga
     4681 cagctaagga aaatgtggaa ctggcttctg aaattgtgac agatgccttg aatcctgatc
     4741 aggtcttgac agatgtaggt ctggctcatc gtctaaataa ctttccagcc cagctttctg
     4801 gaggggagca acagcgagtc tccattgcac gcgcggtagc caaaaatcct aaaattcttc
     4861 tttgtgatga acctactgga gccttggatt atcagacggg caagcaggtt ttgaaaattc
     4921 tccaagatat gtctcgtcaa aagggagcga cggtgatcat cgtgactcat aatggagctt
     4981 tggcgcccat tgctgatcgc gtgattcata tgcacgatgc cagtgtcaag gatgtggtgc
     5041 tcaaccagca tcctcaggat attgacagtt tggagtacta gtatgatcaa gcgaaaaact
     5101 tattggaagg acttaattca gtccttcaca ggctccaagg ggtgtttttt atccatcttg
     5161 atcctgatga tgttgggatc tctagcctta gtaggcctca aagtaaccag tcccaacatg
     5221 gaggcgacag ctaatgctta tttaacaact gctcaaacct tggatttggc agtcatgtct
     5281 aactatggct tggatcaagc agaccaagaa gaactaaaac agacggaggg cgcagaggtc
     5341 gagtttggct atttgacaga tgtgactatg gataatgggc aggatgccat tcggctgtac
     5401 tccaaaccag agcgaatttc aacctttcag ctaagaaagg gacgacttcc tcagtcagac
     5461 aaggaaatcg ctttggccac tcatttgcaa ggccaataca gcgtgggaca ggagattagt
     5521 tttaaagaaa aagaagaggg tcattcctct ttaaaagacc atacttatac cattcctggt
     5581 tttgtggatt cggctgaaat cctctcccag cgagatatgg gctacgcagg aagtggaagt
     5641 gggactctga cagcctatgg ggtgatttta cctagtcagt ttgatcagaa agtctacaat
     5701 atagctcgtt tgaaatatca agatttagca ggtttaaatg tcttttcatc agcttatgaa
     5761 gaaaaatcca agcaacatca ggaagagctt gaacaaattt tatcagataa tggcaaggta
     5821 cgtctgcaac ttttgaaaaa agaaggacaa gagtctctag acaaggggca agagaccctt
     5881 gacaaggctc agactaattt gcaggaaggc aagcgtcgtt tagcagctgc tcaagctcgt
     5941 atacaggctc aagaaagtca actagccttg tttcctcaag ttcagagaga gcaggctagt
     6001 gctcaactta cccaagccaa gcaggaattg ggcaaggaag aggacaaact aaagcaagct
     6061 gaacaaaatc tagcccaaga aaaggaaaaa ttagaaaaac atcagcaagt cttggacgat
     6121 ttggcggagc caaggtatca ggtctataat cgtcagacca tgccaggtgg tcagggctat
     6181 cttatgtata gcaatgcttc atccagtatt cgagcagtgg gcaatatctt tcctgtggta
     6241 ctttatgccg tagcagccat ggtgaccttt acgaccatga ctcgctttgt agacgaagag
     6301 cgaactcatg cagggatttt taaggccttg ggttatcgta gtaaggatat tatcgccaag
     6361 tttctccttt atggactagt agctgggact gtcggaacgg ctctaggtag tatacttggt
     6421 cattatttgc tagccagtgt aatttcaagt gtcattacaa aaggcatggt ggtgggagaa
     6481 acccagattc agttctattg gacctatagc ttactagctt ttgtcttgag cttgttggcg
     6541 agtgtgttac cagcctatct ggtggcttgg agggaacttc atgacgaagc agcccagctt
     6601 ctacttccta aacctcctgt caaaggagct aaaatcttat tggagcgtat cggttttatc
     6661 tggcgtcgtc tcagttttac tcataaggta acagcccgca acatctttcg ttataagcag
     6721 agaatgttga tgacaatctt tggtgtggca ggttctgtag ctctgctctt tgcaggtttg
     6781 ggaatccaat cttctgtagc aggagttccg tctaaacagt ttcaacaaat ccaacagtat
     6841 cagatgcttg tctctgaaaa tcctagtgcg accaatcagg acaaggtaga gctagcagaa
     6901 gtgttgaaag ggcaggagat actagcctac cagaaaatct attctaaagc gctatacaag
     6961 gatttcaaag gcaaagctgg tcttcaaaat attactctta tgatgataga gaaggaagat
     7021 ttgactccct ttatccatct tcaacatcat cagcaggagc tgacattaaa agatggcatc
     7081 gttattacag ctaaactcgc ccagctggca ggtgtcaagg ttgggcagac tttagaaatt
     7141 gaaggtaagg aactaaaggt cgctgctatt actgagaact acgttggtca ctttatttat
     7201 atgagtcagg ctagctatga gcaactttac ggacagctac cccaagccaa cacttatctg
     7261 gtctcattaa gggataccag tgcaactagt atcgaaagtc aggcgggctt gcttatgaat
     7321 caatctgcgg tgtccagcgt tgtccaaaat gcttcagcca ttcgactctt cgactctatc
     7381 gctagctcac tcaatcagac catgaccatc ttggtcatcg tatcggttct attagctatt
     7441 gtcatccttt acaatctgac caatatcaac gtagctgaga gaatccgtga actctccact
     7501 atcaaggttc ttggttttca taataatgaa gtcaccctct acatttaccg tgagacgatt
     7561 gtgctgtccc ttgtgggaat cgtacttggt ctgatagctg gtttctattt acaccaattt
     7621 ttgattcaaa tgatttcgcc tgcgactatt ctcttttatc cgcaggtagg ctgggaagtc
     7681 tatgtaatcc cagtggcagc agtaagcatc attttgacct tgcttggttt cttcgtcaat
     7741 tattatctga gaaaggttga tatgttagaa gccctgaaat ctgtagagta aggtagttat
     7801 ttttagctga ttgaacttct atttactaat attcaaaaat cctccgtttc aaagagcagg
     7861 gaactctttg tgacagagga ttttttctat agggctttag cagctgcaat tgcggcttcg
     7921 aagtttggct cagaattgat attatccacg tattcaacgt agcgaatcgt attgtcagta
     7981 tcgaggacaa agactgcgcg tgctaatagg tgccattcgt tgatcaagag ggcataatcg
     8041 tgcccgaaag aatggtcaaa gtagtctgaa agcataatgg cattgtcaag gccttcagca
     8101 ccgcaccaac gtttttgagc aaaaggtagg tccattgaaa cagtcaatac gaccgtgttg
     8161 tccagtccag ccaattcttc attaaaacga cgtgtttgag ttgagcagat gcctgtatcg
     8221 atagaaggaa cgacactcaa gacttttttc ttgccatcaa aatcagccag agatttttta
     8281 gaaagatctg ttgtagtaag agaaaaatca agcgccttgt cgccgacttg tagttgttta
     8341 cctgtaaagc tcacaggatt tccgagaaaa gttaccatag gatactccaa tcttttttct
     8401 tccattgtat ctgaaacagt cagaattttc caatgatttg accggaaatg tgggcataga
     8461 aaaaacgcca gctcatatga gaatgacgtt tttcagaggc ttattttgcc aatccttcag
     8521 caatcttgtc aaggttgtat ttcatcatgc tgtagtagct gtcgccttct ttaccttgtt
     8581 ctgcgataga gtcagtaaag atttgtgcgt agattgggat gtttgtgtct tgagaaacgg
     8641 ttttcattgg acggtcatcc acacttgatt ctacaaagag tgatggaact tttgtttggc
     8701 gaagtttttc aaccaaggtc ttgatttgtt caggagttcc ttcttcttca gtattgattt
     8761 cccagatgta ggcacttgga acaccatagg ctttagagaa gtatttgaat gctccttcgc
     8821 tggttacaat gagtttcttt tcagcaggga tcttattaaa tttatcctta ctttctttat
     8881 caagtttgtc taacttatca gtatattctt tgagattttt ttcatagaat tctttattgt
     8941 tagggtcttt ggcgctcaat tgtttggcga tatttttagc aaaaataata ccgttttcaa
     9001 ggttaagcca agcgtgtggg tcttcttttc ctttttcatt ttgaccttca aggtagataa
     9061 catcaacgcc gtcgctgact gcgaagtagt ctttgttttc agttttcttg gcattttcta
     9121 ccaattttgt aaaccaagca ttgccacctg tttcaaggtt gataccgtta tagaaaatca
     9181 aatcagcctc agaagttttc ttaacgtctt caggaagtgg ttcgtattcg tgtgggtctt
     9241 gcccaatcgg aacgatacta tgaaggtcaa ttttgtcacc agcaatattt ttagtaatat
     9301 cagcgatgat tgagtttgta gcaacaactt ttagtttttg accagaagtt gttgcttttt
     9361 ttccgctagc acatgctaca agaatgattg cagaaagaaa gagaacgagt aatgtaccta
     9421 attttttcat tagatcctcc aatttattag ggctttgccc cttattttaa caaatgttta
     9481 tttttcagtt tcaaatatcg ttgtttggga gcgataaaga agctaatgag aaagaaacta
     9541 gcagctgtaa gcacgatact agaacctgcc gcaacattaa aactatagcc aataaagagt
     9601 cccaaaactg aagcagtagc tccgaaggtt gaggaaagga aaatcatgct tttcagacta
     9661 ttagcataca gataagcagt tgcagctggg gtaatcagca tggctacaat caggatagtt
     9721 ccgacacttt gcatggctgt cacagacacg agagtcagga gtaccatgag aaggtagtga
     9781 tagaaattga caggcattcc catggcttta gccaagagtt catcaaagga agttatcaag
     9841 agttgcttga agaaaatcca gattaacaag aggatggctg cccccacacc catagtaata
     9901 aacatatccg tatcttggac ggccaggata ttaccaaaaa ggatatggaa aaggtcagtt
     9961 gaacttttag cgacaccaat caagatgata ccgagggcta agaaagaaga aaaggtaatg
    10021 ccgatggcgg tatcgctttt gataatcgag tttcctttga tgtaggtaat gatgatggca
    10081 gctagcaatc caaagacaat ggctccgata aagaagtcaa ggcccaagat gaaggagagg
    10141 gctacacctg gtaagacagc atgtgaaatg gcatctccca tgagtgacat cccgcgtaga
    10201 ataatgaaac atcccacggc tccagctaca atcccgacga caatagctgt tatcaaggca
    10261 ttttgtagga aatggaattt ttgcaatcca tcgataaatt ctgcaatcat aggtcacctc
    10321 cattgaaaaa gagttgatta ccgtaagctt cttttagatt ggtttcggta aaagtttctt
    10381 ttgttggacc aaaggcaatc acttctcgat tgacaagtaa gacttgatcg aagtagtggg
    10441 gaatcttgct gaggtcgtgg tgaacgatga gaaccgtctt cccagctttt ttcaaatctc
    10501 tcagcgtatt catgatgatt tcctcactga cagagtcaat cccagcaaag ggttcatcca
    10561 agaggatata gtcggcttcc tgcaccaaac atctggcaat caagacccgc tggaattgac
    10621 ctccagacag ttgactaatt tgacgttcag cgtagtcagc taggccgacg atttcaaggg
    10681 cctcttgcac tttcttccaa tgtttagcct ttaaacttcg aaagagagga atagagggaa
    10741 atagtcctaa cgagacgcat tccttgacct tgatgggaaa gttgtagtcg atattgattt
    10801 tttgttcgac ataggcaatt cggtgtaagg attttttaac ttccttgtca tcgagaaatg
    10861 cctgaccttg atgtgggata attcccagca taccttttaa tagtgttgat ttcccagcgc
    10921 cgtttggacc aatgatgccg gtaattgttg gtccatggag cactagtgaa atatccttaa
    10981 gtgccaacgt ttctttgtag gagacactga ggttttcgat acgtatcata aacttgtatt
//
LOCUS       DAVLXF010000065        10753 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLXF010000065 DAVLXF010000000
VERSION     DAVLXF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.065"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(46..1362)
                     /locus_tag="VYO12_001570"
     CDS             complement(46..1362)
                     /locus_tag="VYO12_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1588143.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALTQS
                     AFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLMVL
                     LGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFNK"
     regulatory      complement(1453..1541)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1705..4041)
                     /locus_tag="VYO12_001571"
     CDS             complement(1705..4041)
                     /locus_tag="VYO12_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1588144.1"
                     /translation="MNKKILETLELDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLTSREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKVKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYVEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4145..4693)
                     /locus_tag="VYO12_001572"
     CDS             complement(4145..4693)
                     /locus_tag="VYO12_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1588145.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4690..4992)
                     /gene="zapA"
                     /locus_tag="VYO12_001573"
     CDS             complement(4690..4992)
                     /gene="zapA"
                     /locus_tag="VYO12_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1588146.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5079..5960
                     /locus_tag="VYO12_001574"
     CDS             5079..5960
                     /locus_tag="VYO12_001574"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1588147.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFDYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            5964..6578
                     /gene="lepB"
                     /locus_tag="VYO12_001575"
     CDS             5964..6578
                     /gene="lepB"
                     /locus_tag="VYO12_001575"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1588148.1"
                     /translation="MNSLKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKLLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6658..6780
                     /locus_tag="VYO12_001576"
     CDS             6658..6780
                     /locus_tag="VYO12_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEV1588149.1"
                     /translation="MKIKEQTRKLAVGCLKQWFEVVDRTDEVSSKYCFEVADGS"
     gene            6857..9223
                     /locus_tag="VYO12_001577"
     CDS             6857..9223
                     /locus_tag="VYO12_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1588150.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGEQKRQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLTNQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGVALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(9271..10554)
                     /locus_tag="VYO12_001578"
     CDS             complement(9271..10554)
                     /locus_tag="VYO12_001578"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1588151.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         3079 a         2431 c         2184 g         3059 t
ORIGIN      
        1 aaaattattt ataggcaaaa aaatcacacg agctgtgtga ttccattatt tgttaaaata
       61 ctttttagtt tcagcaataa cgactggcga caagaccaag agggcaatca agtttggcag
      121 agccatcaag gcgttaacga tatctgcgat aatccagacc atatccaact cgataaatcc
      181 tcctaacaag accatgagca caaaaaacac acggtagagc cagataaagc gagccccaaa
      241 gaggaactca aaacagcgtt ctccgtaata gttccaacct agaatcgttg taaaggcaaa
      301 aagtacaagg aagatggtca agagggcagg cccaaagtgt gaaaagactg ttgagaaagc
      361 tgactgagtc aaggcaaccc cattcaagtc accactccaa actccagtta ccaagatggt
      421 caaaccagtt agagtacaaa tgatgagggt atcaataaag gttcctgtca tggaaatcaa
      481 accttgctct actggttcat ttgtcttggc agctgcaata ggagcagaac ccagaccaga
      541 ttcgtttgag aacacaccac gcgccacacc attttgaata gccatccgaa cgctagcacc
      601 agcaaatcca cctaccgcag caaggggact aaaagctgag gtaaagacta aagcgattgt
      661 gccagggatt tttccgatat taaagaaaat aactgtaaga gttcctaaga tataaatgag
      721 ggccataaaa ggaacaacag tagttgaaac cttagaaata gacttgagtc caccaaagac
      781 tgcaatcgct acaaagacag acaagacgag agctgtgatg gctggcgaaa tcgtcgttgt
      841 attttggata gattctgtaa tcgagttgac ttgggtgaag gttccgattc ccaagagagc
      901 aaccaatact cctgctactg caaacaaaac agcaagtggt cgccactttt ctcccatccc
      961 tagaaggata tagtgcatgg gacctcccgc tactgcacca tggtcgtcct tggtgcggta
     1021 tttgatggcc aagagtcctt ccgcatactt ggtagccatt ccaaagaaag ccgccatcca
     1081 catccaaaat agagctcctg gtccaccaac cttgatagcc gtcgcaactc ctatgatatt
     1141 tcctgttcca acagttgatg ccaaggctgt acacagagct gcaaaactgg atacatcacc
     1201 atgtccctta tcctggataa aaataagctg aaaggccttg ggcagacgca aaacctgcaa
     1261 gagtcctagc cgcatggtta ggtaaatccc tgttccgacc aataaaatca agaggggcgg
     1321 tccccaagca aaagcatcga ttgatttaag caattctaac atttccttct cctatcgttt
     1381 caaccccaaa agaaagagca catgcaagat acatgtactc tggaatgctt agataaatgc
     1441 taaaaagcgg tctatcctag ctctgtcctt ttacctgaga gtttgagcag ttgcctgcct
     1501 tgccccttcg gtgcctttac ggtctctcca gagttccgtc catttacagt catggaaaat
     1561 caaacgattc cccacttcta ttaaacttca ttcggtgttg gtatttaatt gattctaatt
     1621 ttacaaaaaa tgttggcttt tgtcaatgtg tttattagta aaaattagtt caacagtttt
     1681 tactttataa agtccagaat actgctatcc tttaaaagtg acaatagtcg caccactgcc
     1741 tccagcattt tgtggggcat agccgaaact cttgacatgt ttgtttcttt gcaagtattt
     1801 ggtaactcct tcacggatga ctcctgttcc gataccatgg atgatatcaa cttgagccat
     1861 attgttaagc aaggcttggt cgatgaaggt atctagctca ttcatggctt ctacatagcg
     1921 cttgcctcga agatccagtc tagcttgagg tcctcgccca gaagttcgtt tcacaacatt
     1981 gacctgtttc ttcttgactg gtttttcttg ctgggcttga acaagatcaa actctttctc
     2041 ttccaaggtc atcttaatca agccaacttg ggcttcccag cgaccgtcct tgagttgact
     2101 ggtcaaggta ccacgctgac cataactgag aaccacgata tcatctccca cctttggagc
     2161 tcgttttttc ttggcctttt gaaggacctt atttttagac aagtccactt tttcaggagc
     2221 caattttttc aacttgacct tggcttcaat gatttcgtgg ggcttgagtt gggatttact
     2281 gtggagattt ttgagaatct ggtcactttc acttaaggcc atatccacaa tctcagcagc
     2341 ctgttcacgc gccttgttaa gctcggtttc cttttcacga ttaagctcgt tgtagagttt
     2401 ttttagcgca cggttcatct tgagattttc ttgctccacc tcacggatat tgtccaaacg
     2461 tttgcggctt tccagcgtct gctcttctaa ttgctcaatg atacgattga cgtcattgtc
     2521 ctgatcgatc tgctgactgg catctcctac gataacttca gataggccta gacgtttggc
     2581 aatttcaaag gcattacttc ggccaggaac accctgcata aagcgatagg tcgggcgaag
     2641 agttgcagta tcaaactcca tactggcatt ttgcacaaag gctgtctcaa taccgtaggc
     2701 cttgagttct ggatagtggg tcgtcgccat ggtcttgatt tgacgcaggc gaaggtcctc
     2761 cagaatagcc atggcaaggg ctgctccctc ttggggatca gtaccagccc ccaactcatc
     2821 caaaagtaag agtgaatgtt ggttgacctt gccaagaata tccacgatat tggtcatatg
     2881 gctagagaag gtagacaagc tctgctcaat agactgctca tctccaatat cagcaaagat
     2941 ttcttcaaaa ataccaacac gacttccctt gtctgctaaa atcggcaatc ctgactgggc
     3001 catgacctgt gtcaagccca gagttttgag catgatggtc ttcccacctg tattgggacc
     3061 tgtaatgaca atagctgtta aatcttgacc aaaatagaca tcatttgcga cggcattttt
     3121 gaccaaagga tggcagacat ggagcagttg aatctcttga ttttctgaca gctgaggcac
     3181 gactgcttgt ctttcttgga taaatcgaac cttggcacga atcaagtcca gatgaccgat
     3241 aatccaagcg tcattagcaa tctcagccgc atgagggcgg acacgctcag aaatttcttg
     3301 gagaatgcga agcatttcat agcgctcatc tgctcgcaga ctagcaattt cttcgctcag
     3361 tttgactacc tcacgtggtt cgatatagac ggtgtttcca ctagcagaaa tatcatgaac
     3421 gacacctgca atcttattgc ggtaggtgtt tttgactggt aaaacctgac ggccatttct
     3481 gctagcaaca attccttccg tcaacatctg cgctttttgc ttgagcaagt cttgtaaaac
     3541 atcgcgtacc tgactctcgc tatcatgtat ttttcgacgg attcgcgcca attcttcact
     3601 ggcaaaattt tcaatgaaac ccgcatcatt aaaggcctga agatttcctt gtaattgcgg
     3661 aaaatcatgt aatttctcaa accaaagggc taattcttcc aagctgacat tttccagatt
     3721 ggcgtaaaaa ctttgaagtt ctcggctggt aagaagcacg cgtttcaaga gtaggaactc
     3781 ctcgatattg agatccgctc ccatctccaa cctcttgcag actcctgcaa tttccttagt
     3841 tgagagaata gtaaaatgcg gttgctcgac gaaaagagcc tgcatttctt tcatctcagc
     3901 aaaagcctgt ttgattttat ctgctttggc agtcggagcc agctgtctca attgctccaa
     3961 gccctgctcg gtcaacaaat gaggctcaaa caaggccttg accttatcga gctctaatgt
     4021 ttctaatatt ttcttattca tctttatttc cttgtctttg aatgtctagg tgtggtagct
     4081 ttttacattc tgatagattc gagtcaaatc tgtaaacaaa gggctaggaa aactcctgcc
     4141 ctttttatcc gattaaattt gtcacccaga tttgtttgag ccaactggtt gttatcggta
     4201 tactctggat gatgtgtttt gcgacgatac tcttttcaag aggattttgt ataactgcca
     4261 tggggatggt cgccaagatt gtcaaggcca tttgcaagac aaataaggtc accaacatgg
     4321 acaagatacc tgctgaaact tggaacaact taccacccag ttttttgcta ggaatcaagt
     4381 gtaagagaag accaagcaaa cgaccaatgc tatagacaat cccaaataca agcaagtagc
     4441 cgatacctgc ataaaagacc ttatccagct gaaagagttg atccgatggg aaaaagaaag
     4501 tcccctgacc ttcctgcgaa tttgcataag ggagcaataa atggaattgc tctccaagcc
     4561 ccttataaaa ctggccagcc ataaaagccg atgccatggc tgaaatcagg taataaacct
     4621 gtaagagcag gcctctccga tagccgatat aaaatcccca aaccaagacc aatagaagaa
     4681 ggaatgaaat cataaggaat cctcaatctt gctctgttct tgcttacaag tcacaagctt
     4741 gtgacggagt tcttctagct cttgctcctt atcgtcaaat tcaatctcac ggctgagctg
     4801 agttgataaa cagttgactg ccaacaaaag agcgattgtt tcatcatctg cgctaggcat
     4861 ttgttcttta attgcttggt atttttctgt cgcaacctta gcgatttcct ccataaaaag
     4921 gttatcatgt tcgcttgtca aggttaacga ttttttcccg aatgtaaatt tgaatcgatt
     4981 tagatttgcc ataaaattca cctcacgata ttataccaaa attcgctaat tttgtcagtt
     5041 tttacaaatt ttactgcttt tgtggtacaa tagaaactat ggcaagtatc acactcacac
     5101 caagcgaaaa ggatattcag gcttttcttg aacactatca aaccagtctg gctcctagca
     5161 agaatcccta tatccgctac tttttgaaac tacctcaagc aacggtttct atctatactt
     5221 ctggaaaaat cttgcttcag ggtgaagggg ctgaaaaata cgccagtttc tttgactatc
     5281 aagctgtaga gcaaaccagc ggacaaaatc ttcctttaat tgggacagat gaggtgggaa
     5341 atggttccta ctttggtggg cttgcagttg tggctgcctt tgtcacacct gaccagcacg
     5401 actttttacg aaaactcggt gtgggggatt ctaagactct gaccgaccaa aagatccgtc
     5461 agattgctcc tattctcaag gaaaaaatcc agcaccaggc actccttctc tcacccagca
     5521 agtacaacga ggtcatcgga gaccgctaca atgctgtttc ggttaaggtt gccctccata
     5581 atcaggctat ctatctcctc cttcaaaaag gtgttcagcc tgagaaaatt gtgattgatg
     5641 cctttaccag tgctaaaaat tatgacaagt acttggcaca agaggccaat cgtttcagca
     5701 atcctatcag cttagaagaa aaggctgagg gcaaatactt ggctgtcgca gtttcttctg
     5761 tcattgcgcg tgatctcttt ctggaaaatc ttgaaaattt gggacgagaa ctgggttatc
     5821 agcttccaag tggagctgga acggcttctg acaaggtggc tagccagatt ttgcaagcct
     5881 atggtatgca gggactcaac ttctgcgcca aattgcactt taaaaacact gaaaaagcga
     5941 aaaaacgctt agaaaggtaa gttatgaatt cacttaaaaa tttcttaaaa gagtggggat
     6001 tgttcctcct aattctgtca ttactagctt taagtcgtat ctttttttgg agcaatgttc
     6061 gcgtagaagg acattccatg gatccgaccc tagcggatgg cgaaattctc ttcgttgtta
     6121 agctcctccc tattgaccgt tttgatatcg tggtggccca tgaggaagat ggcaataagg
     6181 acattgtcaa acgtgtcatc ggaatgcctg gcgataccat tcgttacgaa aatgataaac
     6241 tttacatcaa tgacaaagaa acggacgagc cttatctagc agactatatc aaacgcttca
     6301 aggatgacaa actccaaagc acttactcag gcaagggctt tgaaggaaat aaaggaactt
     6361 tctttagaag tatcgctcaa aaagcccaag ccttcacagt tgatgtcaac tacaacacca
     6421 actttagctt tactgttcca gaaggagaat accttctcct cggagatgac cgcttggttt
     6481 cgagcgacag ccgccacgta ggtaccttca aagcaaaaga tatcacaggg gaagctaaat
     6541 tccgcttctg gccaatcacc cgtatcggaa cattttaaga gacctaagag gccgagaatc
     6601 acaaatctca gcctcttctt ctatcgtgag aagtgattgt ttattgacaa atactcaatg
     6661 aaaatcaaag aacaaactag gaagctagcc gtaggctgct taaaacagtg gtttgaggtt
     6721 gtggatagaa ctgacgaagt cagctcaaaa tactgttttg aggttgcaga tggaagctga
     6781 cgtggtttga agatattttc gaagagtata aaataaaaac aatcccaact ctcacctatt
     6841 catgcaaagg aattttatgg aagtttattt ttcaggaacc attgaacgga ttatttttga
     6901 aaatcccagc aatttttatc gcatcctcct cctagaaatc gacgatacgg acgcagagga
     6961 ttttgatgat tttgaaatca ttgtcacagg aaccatggct gatgtaattg agggcgaaga
     7021 ctatactttt tgggggcaaa ttgtccagca ctccaagtat ggagaacaac tgcaaatcag
     7081 tcgttatgat cgcgcaaaac caactagtaa gggattggtc aagtactttt caagtagcca
     7141 tttcaagggg attggtctca agacagctca gaaaatcgta gatacctatg gcgaaaatac
     7201 cattgatgaa attttgcaac acccagaaaa gttagaaggc atcgcaggac tctctgccaa
     7261 aaatcgcgag gctttcgtct ccactctccg tctcaactac ggaacggaga tggttttggc
     7321 caaactagcc aactacggca ttcccaacaa actagccttt cagattcaag acttttacaa
     7381 ggaagaaacc cttgatgtgg ttgaaaatta tccctaccag ctggttgagg atatcaaggg
     7441 tttgggcttt acaattgctg accaactagc tgaggaacta ggcatcgaaa gtcaggctcc
     7501 tgaacgcttc cgcgccggtc tagttcacag tctttttcag gcctgtatgg aaacaggaga
     7561 cacctatgtt gaagcacggg atttgctgga acaaaccctt actctccttg agtcttcccg
     7621 tcccgtagaa ctagacccca gccaagttgc ccaagagctc tcctacctga tcgaagaaga
     7681 caaggttcag cagattgata ccaagatctt tgacaacagc ctctttttcg ctgaggaagg
     7741 tatccgcagt cacttgattc gtatccttga aaaaggagaa cagaagagac aagatttaga
     7801 aactattcaa aagcatatca ctactgtcga gcaagaactg gggattgagt atgataacat
     7861 tcaaaaacaa gctatttgtg acgctatcca gaacaaggtc tttatcctga caggtgggcc
     7921 tggtactggt aagacaactg ttatcaatgg aatcatcgct gtttatgccc ttttagaagg
     7981 acttgacttc aggaagaaaa gcaatctgcc cattcttctt gctgctccaa ctggtcgagc
     8041 tgctcgtcgc atgaatgaat tgacaggttt gcctagcgcg accatacatc gccacttggg
     8101 aatgacaggt gacgatgata ccagtcatct ggaagattac ctagatgctg actttatcat
     8161 cgtggatgaa ttctctatgg tagatacttg gctgaccaat caactcttct ccaatatctc
     8221 ttctaacagt aagatcctca tcgtaggtga cagtgatcag ctaccttctg tcagtcctgg
     8281 acaggttcta gcggatctgc ttcatattcc tttgattcct cagactcgct tggaaaaaat
     8341 ttatcgacaa agcaaagaat caaccatcgt caccctagct agtcagattc gacagggcat
     8401 cttgccagct gatttcaccc aaaaaaaagc tgaccgttcc tactttgaaa ttgctagtgg
     8461 ccatattcct gccaccattg aaaaaatctt aggtgtcgcc ctcagaaatg gtattcctgc
     8521 ccgtgatatc caagttctgg ctcctatgta ccgagggacg gcagggattg atgctatcaa
     8581 tcagctcatg caagacctgc tcaatccccc acaaaaagat caactcagtt ttgaagctcc
     8641 tcagtgccac tatcgtaaga gagacaaggt cattcatttg gttaacgatg ctgaaatcaa
     8701 tgtctttaat ggggatttag gagctatcac agacctgatt cctggtaaat acaccgaatc
     8761 gaaacaagac gagattgtca ttgattttga tggtaatgaa gtctcttacc cccgtaacga
     8821 atggtacaag attcgcttgg cctatgccat gagtattcat aagtctcagg gaagtgagtt
     8881 cccagttgtc atcctaccga ttactagtgc tagccgacgt atgctggagc gaaatctcat
     8941 ctacacagcc attacacgtg ccaaaagcaa actcatctta ctaggcgaat tacaagcctt
     9001 cgactatgct acccaacaca tcggaactgc ccgaaaaacc tatctgattg aacgcttcag
     9061 tgatctactg gagaatgttg aagaaaagca acaagctgtc tctgaaacag tcacatcaag
     9121 tgcctctgaa caatcctaca tcctaaccga agaaaactgg gacagcattc cagccatgat
     9181 tgggattaca gacacagacc tcaaagagat ttttggaaaa tagtacatca gaaaacccac
     9241 caattcaggt gggttttctg tttattaaga ttattttact gttgctgtgc ttgtgatcaa
     9301 ttcaacagct tttttgatag tgatatcatg tttcaacatg tcagctgaaa gcaagttttg
     9361 aacttgtgca acttccatgt tgtagtctgc tgccaattgc tcaacttctt tttggatttc
     9421 ttcttctgaa gcatcaaatc cttcagcttt ggcaactgct tcgataacaa ggttagtctt
     9481 agtacgtgac tcagcttctg cttggtattg gttgtgaagg tcttcttgag tagttccagt
     9541 gatttggaag tacatgtcag ggttgatccc ttgacgttgc aagttcccaa ggaattcatt
     9601 tactgaacgg tgaacttctt catggatcat ttcttctgga agttctacga tttcagcgtt
     9661 ttctacagct gtatcaattg ctgcaccttc aactgcatct ttgtaagctt cttctttagc
     9721 agcagccaat tctttgcggt atttttcttt caagtcagca agtgtttcaa cttcttcatc
     9781 aatgtctttt gcaagttcat cgtcaagagc tggaacttct ttagctttta cttcgtggat
     9841 agttgtcacg aatttagctt ctttacctgc aaggtcttct gcttggtagt cttctgggaa
     9901 tgttacgata acatcaacgg tttcgccagc tgagtgacct accaattggt cttcgaaacc
     9961 agggatgaat tgacctgaac caagtccaag tgagaagttt tcaccttttc caccgtcaaa
    10021 ttcaacaccg tcgatagaac caacgaagtc gatcacaaca gtgtcgccgt tttcagcagc
    10081 agcttccttg ataaccaatt cagccagatt gttgcgttcg cgttcgatac gctcttcaac
    10141 atcagcgtca gttacttctt tttctacatc aactgatact tcaaggtttt tgtagtcacc
    10201 caattttact tcaggttttg taacgacttc agcagcgata acccagtctt gacctttttc
    10261 cattgaagtt acgtcaattt ttggttgggc aaccacttca agaccagctt cttttacagc
    10321 tgcttcataa gcgtttggca aaagagcgtt cataacgtct tggtaaagtg attcttcacc
    10381 aaattttttg tcgaagatag gacgtggaag gtgaccttta cggaaacctg gaacattaag
    10441 agatttcttc actgacttga agacacggtc caattctggt ttgatttggt cttgagagat
    10501 agtgaaagtc aagacaccac ggtttgtttc tttgttttca aatgatacag acattctgtc
    10561 atttctcctt aaaatttttt aaatacagtc tattataaca ttttctataa ataaaatcta
    10621 gactaatgca gccaattata gacaacaagc ctttttccta actaaaacac tcataaagta
    10681 tagacaattt tagactactt tggatatctt ttggttagtc attttccaaa actaatttat
    10741 ctaatttttc agt
//
LOCUS       DAVLXF010000066        10659 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLXF010000066 DAVLXF010000000
VERSION     DAVLXF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.066"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..651
                     /locus_tag="VYO12_001579"
                     /pseudo
     CDS             1..651
                     /locus_tag="VYO12_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            617..997
                     /locus_tag="VYO12_001580"
     CDS             617..997
                     /locus_tag="VYO12_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1588152.1"
                     /translation="MRLFCLVLVSIDYINCLSETTDKNWHKSDRVFVTNTGKTVHSSI
                     LSKSLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVGHSDSEVTTSI
                     YTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1135..2439)
                     /gene="eno"
                     /locus_tag="VYO12_001581"
     CDS             complement(1135..2439)
                     /gene="eno"
                     /locus_tag="VYO12_001581"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1588153.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2603..3049
                     /locus_tag="VYO12_001582"
     CDS             2603..3049
                     /locus_tag="VYO12_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1588154.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3046..4161)
                     /locus_tag="VYO12_001583"
     CDS             complement(3046..4161)
                     /locus_tag="VYO12_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1588155.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASDQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4170..4499)
                     /locus_tag="VYO12_001584"
     CDS             complement(4170..4499)
                     /locus_tag="VYO12_001584"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1588156.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4444..4815)
                     /locus_tag="VYO12_001585"
     CDS             complement(4444..4815)
                     /locus_tag="VYO12_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1588157.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4874..6307)
                     /gene="glgA"
                     /locus_tag="VYO12_001586"
     CDS             complement(4874..6307)
                     /gene="glgA"
                     /locus_tag="VYO12_001586"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1588158.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHIHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWLAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6304..7443)
                     /gene="glgD"
                     /locus_tag="VYO12_001587"
     CDS             complement(6304..7443)
                     /gene="glgD"
                     /locus_tag="VYO12_001587"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1588159.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7433..8575)
                     /locus_tag="VYO12_001588"
     CDS             complement(7433..8575)
                     /locus_tag="VYO12_001588"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1588160.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8565..10493)
                     /gene="glgB"
                     /locus_tag="VYO12_001589"
     CDS             complement(8565..10493)
                     /gene="glgB"
                     /locus_tag="VYO12_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1588161.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPNVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLICIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3241 a         2339 c         1888 g         3191 t
ORIGIN      
        1 aaaactatct tactgacaat ccagacgctt atttgactga aatagcttct gaatttggct
       61 gtcatccaac taccatccac tatacgctca aagctatggg ctacactcga aaaaaagaac
      121 cacacctact atgaacaaga cccagaaaaa gtagccttat ttcttaagaa ttttaatagt
      181 ttaaagcacc tagcacctgt ttagattgac gaaacaggat tcgatactta tttttatcga
      241 gaatatggtc gctcattaaa aggtcagtta ataagaggta aagtatctgg aagaagatat
      301 cagaggattt ctttggttgc aggtctaaca aatggtgagt taatcgttcc aatgacttac
      361 gaagagacga tgacgagcga cttttttgaa gcttggtttc agaagttttt cttaccaaca
      421 ttaaccacac catcggttat tattatggat aatgcaagat tccatagaat gggtaagtta
      481 gaactcttgt gtgaagagtt tgggtataaa cttttacctc ttcctcccta ctcacctgag
      541 tacaatccta ttgagaaaac atgggctcat atcaaaaagc acctcaaaaa ggtattacca
      601 agttgcaata ccttttatga ggctcttttg tcttgttctt gtttcaattg actatataaa
      661 ttgtctaagc gaaacaaccg ataagaattg gcacaaaagc gaccgtgttt ttgttaccaa
      721 tacaggaaaa acagttcata gttctatctt gagcaagtct ctccagcgag caaacgaacg
      781 ccttaaaaaa ccaattccca aacatctgtc ccctcacatc ttcagacaca ccactattag
      841 catcttatca gaaaataaaa ttcctttaaa aacaatcacg gacagggttg gtcattccga
      901 ctctgaagtc actacttcca tctacaccca cgtcacaaag aacatgaaag atgaagcaat
      961 caatgtactg gataaagtta tgaaaaagat tttttaaaaa gttttgcccc ttttttgtcc
     1021 tctaaataca aaaatagccc ttcggataaa atccgagggg ctagaaacgt tgttaaatca
     1081 acggccgaac ttttgaattt catggttcgg gataaaatag ttcactgaac tattttattt
     1141 tttaaggttg tagaatgatt tcaatccacg atattcagct acttcaccaa gttggtcttc
     1201 gatacgaagc aattggttgt atttagcgat acggtctgta cgtgaaagtg aaccagtctt
     1261 gatttgtcct gcgttagttg caactgcaat atcagcgatt gttgaatctt cagtttcacc
     1321 tgaacggtgt gatacaacag cagtgtaacc agcttcttta gccatttcga tagcttcaaa
     1381 agtttcagta agagtaccga tttggttaac tttgataagg attgagttag cagcaccttc
     1441 ttggatacca cgtgcaaggt agtcagtgtt tgttacgaag aagtcgtcac caacaagttg
     1501 tactttctta ccaagacgtt cagtaagagc tttccaacca tcccagtcgt tttcatccat
     1561 accatcttca atagtgatga ttgggtattt gttaaccaat tcttcaaggt agtcgatttg
     1621 ttctgcagat gtacgaacag cagcaccttc accttcaaat ttagtgtagt cgtaaacttt
     1681 acgttcttta tcgtagaatt ctgatgaagc acagtcaaat ccgataaata cgtctttacc
     1741 tggtacatat ccagcagctt caatcgcagc aaggatagtt tcaacaccat cttcagttcc
     1801 ttcgaaacga ggagcgaatc caccttcgtc acctacggca gtttccaaac cacgtgattt
     1861 aaggattttc ttaagagcgt ggaagatttc agcaccgtaa cgaagggctt ctttaaatgt
     1921 tggcgcacca actggcaaga tcatgaactc ttggaaagcg attggagcgt cagagtgaga
     1981 accaccgttg atgatgttca tcattggagt tggaagaact ttagtgttga atccaccaag
     2041 gtagctgtaa agtgggattt caaggtagtc agcagcagca cgagctacag cgatagacac
     2101 accgaggatt gcattcgcac ccaatttacc tttgttagga gtaccgtcaa gtgcgatcat
     2161 agcacggtca atagcttgtt gatcacgtac atcgtagcca atgatagctt cagcaatgat
     2221 gttgtttacg ttgtcaacag ctttttgtgt accaagacca ccgtaacgag atttgtcacc
     2281 gtcgcgaagt tcaactgctt cgtgttcacc agtagaagct cctgatggaa ccataccacg
     2341 tccgaaagca cctgattcag tgtaaacttc tacttcaagt gttgggttac cgcgtgagtc
     2401 taggacttcg cgagcgtaaa catcagtaat aattgacatt ttttactctc cttatgagtt
     2461 aaatttttta cacctctata ataccttaaa acccctcctt tttcaagaaa aaacgttatc
     2521 tttgtgcaac ttttccttaa ctttataaag taatcgcttt cttttgtctg ttttattcta
     2581 acttttatga tatactgttt tcatgacaga tttatcaaaa caattacttg aaaaagctca
     2641 tggtgggtta aaaataaatc cggatgaaca aagacgctat cttggtactt ttgaggaaag
     2701 agttcttgga tatgtagata ttgacacagc aaatagccct cagttagaaa aaggcttttt
     2761 atttatttta gaaaaccttc aggaaaaagc agagccacta tttgtgaaga tttcaccaac
     2821 tatcgaattt gataagcaag ttttctactt aaaagaagca aaagaaactg atagtcaagc
     2881 caccatagta tctgaagagc atattacttc tccttttggc ctggttattc atagcaatgc
     2941 accagttcaa gtagaagaaa aagaccttcg acttgctttt ccaaaacttt gggaagttaa
     3001 aaaggaagaa ccagccaaaa catccttatg gaagaaatgg tttagctaaa tcttgcacat
     3061 atttaataag tgcccaatat tggcagccgt gcgctccaga tagagactgg catttttcaa
     3121 actatcttct aaaggttcac ttttctccaa aatagaaaag acagcttgga tattttcaaa
     3181 tggtagggga ggtaaatctt cagcaagact accgcaaata gcaataacag gaactccaac
     3241 aggggttctt tttgcaacac ctataggcgc tttcccagca aagctttgac tatcaagtct
     3301 tccttctcca acaacaacca agtcagcatc tgaaactttc ttatcaaagt tgattaagtc
     3361 caagcaggta tcaattccag acacgatact tgcctgagca aaggcacaca aaccaccagc
     3421 aaggcctcca cctgctcctg ctcctttaat ttctaatgtt gcaggtgaga atttttcata
     3481 aaaatcttgg atcgcctgat ctacgactgc aaacatagtc ggatgtagac ctttttgatt
     3541 gccaaaagtg taagtcgcac cttgatgacc acataaggga ctcacgacat ctgctagaat
     3601 atgaatttga acaccttcag gaattttata gcaattttct gttgaaacag aagctaagtt
     3661 taataaggat tgatcggaag caggcaagac atttccatcc ctatcataaa attgataacc
     3721 taaaccagca gcaatcccca gtcctccatc attactggcc gtgccaccaa caccgatata
     3781 aatatcttta atccctttag aaatgagatg gagaatcaac tctccaatac cacaagtttg
     3841 gatttgaagt ggatttcgtt tctctagcgg aatttttcca agaccaacca agtcagctac
     3901 ttcaaatagt gccagttccc ctttttgaaa atagcgcatg gcttcttttt gtccaaaagg
     3961 gtctgtcact tggatccatt tttcttttag gtcaagagaa tgtcggatag catctacagt
     4021 accttctccc ccatcaccaa cagggcagag gagacattct acatctgcta tcgattgttg
     4081 gaagcctctt tttattgctt cagctacctg ttgagctgtc aagctttcct taaacgaatc
     4141 cggtgcaatt acaatcttca tattttccct cattctaaac agtcaatcaa aggaagaact
     4201 tctaaaaaat ccctcttgtc aacatgatgt ggtatttctt ttttgagcat ttctttggaa
     4261 caaaaggcga ttcctaactc cgccgacttc aacattaata gattattgac cccatcaccg
     4321 attgccaccg ttctttcttt agaaagtttt agtttctttc tccatttttc cagagtttct
     4381 tttttgacct ggggacttat aatttgtcca actaattttc ctgttaaaag accttctttg
     4441 acttcaagct ggttggcagt gaaataggca ataccaaggg attttgctaa tctctccaac
     4501 tattggtgta aatccaccag acaccagacc aactaggatg ccattctttt ggagaataga
     4561 gatgaactct gggacattta gcgatagatg aattgagttg aagacgttat caaagaccaa
     4621 aataggaaga ccttccaaca aggacactct ttttcttaaa ctgctttcaa agaccaactc
     4681 tcctcgcatt gctcgacttg taatctgcga aatttccgcc tcatgacctg cctctctccc
     4741 taaaagatca atcacttctt ctaggattaa ggttccatct acatccaaaa cacacaagcc
     4801 ttttacttga gacatcagtt ctcctctcta aacagcctaa aaatcgtatg aagtcatcat
     4861 acgattttat ctattaatta actaaactat ggtacaagtc aaggtatgac ttgcaggctg
     4921 tatcccatga gaagtcactc tccatagctt gtttttgtag gtttctccaa atgtctggat
     4981 ggtttctata caagtccaat gctgtttgga aagtccaatt taaccaataa ggagatagat
     5041 tgtcaaagct aaagccagta ccgcttcctt cgattggatt gaaagcgcga actgtatctc
     5101 gcaagcctcc aacttcatgg accaatggca aggttccata acgcatagcc atcatttgag
     5161 acaagccaca cggttcaaaa cgacttggca tgaggaagag gtcacaagca gcgtagattt
     5221 cctgagcaag tttgacatca aaagtgatat ttgttgatag cttgtctggg taaatctgag
     5281 ccaaccatga gaaagctcct tcaaaggctg gatcgccagt tcccaaaaga acaatctgaa
     5341 catcttcttg caagatatgg tgaagacttt cgaccaccac atcaaaacct ttttgacgtg
     5401 tcaaacgaga aacaattccc accagtggaa cgtctgctct aacaggcaag ccaactcttt
     5461 cttgcaattt tgccttattt ttggctttcc cagacaaatc ttcctgattg aaatgatagt
     5521 ctaaaagagc atccgtctga ggattataaa gatcagcatc aatcccattc acgataccag
     5581 atactttacc agactccatt ttaagaatct gatccaaatt acatccaaac tgactagtca
     5641 taatttcatg agcatagcta ggtgaaacgg ttgaaacacg gttcgcatag agaatacctg
     5701 ccttcatcca gttcagacag ttgttccatc gaagggtgcc atcagcgtaa cgttcaaggc
     5761 caactccaaa caaatcaccc aacattcctt ctgaaaattg tccttggaat tctaaattat
     5821 gaatggttaa aactgtttca atgtcctcat aggcttgaat ccaacggtat ttttccttca
     5881 acaagaaagg aatcatagct gtatggtagt catgaatatg gagaagatca ggaataaagt
     5941 caatcctttc catagcctca atggcagcca gttggaaaaa ggcaaagcgt tctccgtcat
     6001 caaaatcacc gtaaacatga ccacggaaga aataatattg attgtcaata aagtagaagg
     6061 ttacaccatt taatactgtt ttcttaattc cacaatactg tctgcgccaa ccaacgctca
     6121 cctcaaaatg aagcacatct tcaatctgat ttccaaattt agcctctacc atatcatagt
     6181 agggtaaaat cactgcaact tcgtgcccag cttttaccag tgattttgga agagcgccaa
     6241 tgacgtctcc caaaccacct gtttttgaaa agggtgcacc ctctgctgct acaaataaaa
     6301 ttttcatgaa tgaatatcct ctgttacttt agcacctttc ttaaccacaa ctggatgttc
     6361 tgcagttcct cgaatcacaa caccaggctc aacttcaacc cctttgtcca agatagcata
     6421 ttcgacctga gccccttctc caataacaac acgagggaag agcaggctat ctttaaccaa
     6481 gctatcctta tggacatgaa tattacgtga tagaacagaa ttagctactt gaccttcaat
     6541 aatactacca gaggcaaact gagaagtgct taccttagat gtattagcat agtaagttgg
     6601 ctcttcgttt ttgacctttg tataaatctt ttggtttggt gagaaaagag aatagaattt
     6661 ttgtgattca agcatatcga tattcgcttg ataataagat ttaacagagt gaatattggc
     6721 tagatagccc gtgtactcgt aggcgaaagc tccctctttt acagccaaat cccgtaaaac
     6781 atagcgcaat ttctttggat gttctttttt agcttcttct tccaagtgtt caatcaacca
     6841 aggtgtatca acgacaaaga tatctgtaga catattgaac gtttcagctg ttgacttgct
     6901 atcaaagagt ttatgagaaa gaacatggtc tgtttcatct acatccaaga ttgcatttac
     6961 ttctgaaata tctttcttag ctagtttttt ataaactaca gtgataggct cttttgttgt
     7021 actatgtagg tggaaaactt ggttcaaatc aatgttaata agaacatcgc agttgagggc
     7081 aaccgtttgg tttgagccag aacgtttcaa ataagtaaga agctgttggt agtattcttt
     7141 tccaactgta ctactttcta cacgggtatt gtaaattcct agatagtaat gactaagaag
     7201 ggttgataag ccccactcgc gtcctgaacg aatatggtca aatactgagc tgatattatc
     7261 ctgctggaaa ataccaaaga cactacgaac acctgcatta gcaaggcttg aaagtgggaa
     7321 gtcaatcaaa cgatatttcc caccaaatgg caaacttgct actggacggt ggtccgtcaa
     7381 tgtcgacata ttgtgaaaac caactgtatt tcctaaaatg gcagaatatt tatcaatctt
     7441 catctgttgc tacccccact acttcattat atcctacaac ttgtacttca tctgttccat
     7501 caatttcgac accgtcagaa ataatcgcac cttcaccaat aatggcacgt ttaatcttag
     7561 ctccttgacc aatgatagct ccactcatga taactgaatc aaggacttcc gctccttcgc
     7621 gaacttgcgc gcctgttgaa aggatagaat gtttaacagt tccatcaacg aaacatccgt
     7681 ctacaactaa tgagtcttcc acatgagcat ttgccccgag gaagtttggt ggtgaaatca
     7741 agtttcttga gtaaatcttc cattgacggt tacgactatc caaggcattt tctggagaaa
     7801 tatactccat gttcgcttcc caaagtgact caatagtacc aacatctttc caataaccac
     7861 taaattcgta agcataaaca ctttcacctg actcaaggta atttggaatg acatttttcc
     7921 caaagtctga catgccaacc ttgctctttt cagcagcgac taacatatta cgaaggcgtt
     7981 gccaatcaaa aatgtagatt cccatagaag cttttgtaga tttaggttga gctggttttt
     8041 cttcaaattc aacaatacga ttgttagcat ctgtgttcat gataccaaaa cggcttgctt
     8101 ctttaagagg gacgtctaaa actgctactg tcaagctggc attattatcc ttatgagact
     8161 ggagcatatc atcatagtcc attttgtaga tgtgatcccc agacaaaatc aagacatact
     8221 caggattgac actgtcgata tagtcgatat tttggtaaat agcgtgacta gtcccctcaa
     8281 accaacgatt tccttcactt gcagaataag gttgaagaat agagacacct gaattgatac
     8341 cgtctagtcc ccagcttgaa ccattcccaa tatggttgtt gagagcaagt ggttgatact
     8401 gtgtaacgac cccaacatta tgaatccctg agttggcaca gtttgatagg gcaaagtcaa
     8461 tgatacggta gcgcccacca aattgcacag ctggttttgc gatgctttga gtgagtttac
     8521 cgagacgagt tccttgccca ccagcaagaa tcaaagctaa catttcattt ttcattttct
     8581 actccttttt ggtttttatt tgtgacggtt ttagtagatt tcaagcgacg tttgattttc
     8641 catacacttg ctcccatagc cggtagagta aaggttaagg tctgctcata atctttccat
     8701 agtccttctt gcgtttgaac agtttgatta tgttctttcc aaacgcctcc ccactcttcc
     8761 aactcagtat tccatacttc ttcgtaaatt cctgcaacgg gtagtccgat tgtaaaatct
     8821 ttccgctcaa caggtaccat gttaaagata cagattaaca tttctccctt tttaccctta
     8881 cgaataaagg aaagaacact ctggtctcga ttatccgcat caatgatttc aataccatca
     8941 tagctggtat cgatttccca cagacagcga tgatctttgt aaaactggtt tagctgagaa
     9001 gtgaaatact tcatcttagc attcattggg tcttctaggt tagaccattc caactgttct
     9061 tcagatttcc attctaggaa ttgaccgtat tcgctaccca tgaagagcaa tttcttacca
     9121 gggtgacaaa tttggtacgt atagagattg cgcaagcctg cgaattgatt gtaacgatct
     9181 ccccacatct tatgcatcat actcttcttg ccatgaacca cttcatcgtg cgagaatggc
     9241 aagagataat tctccttgaa aacatacata aagctgaaag tcaccaggtt aaagtcatat
     9301 ttacgataga tcggatcttc ttcgtagaaa cggaggatat cattcatcca gcccatgttc
     9361 catttgtagt caaatcctag accaccaatc tctttcattc ccgtaatctt ggtcgcagac
     9421 gaactttctt ctgcaatcat catcacattt ggatattcta acttaataac ctcattcaag
     9481 cgctgaagga aataataacc ttcatagttg agatttccgc catctttatt aggtgtccat
     9541 ggagcatcat catagtccaa atagagcatg ttgctaacag catccacacg aataccatcc
     9601 aaatgataga catcaatcca atgcttaatg caagaaatta agaaggactg gacttcattt
     9661 tttccaaggt caaaattaag ggcaccccaa ccatggttat gagccttatt atggtcttgg
     9721 tattcaaaag tcggtgtccc atcataatag gctaaggcat catcgttgat ggtaaagtga
     9781 cctggtaccc agtccacaat aaccccaata ttatgggtat gacactcctc gacaaaatct
     9841 tgaaactcct ctggtcggcc ataagcatgc tctaaagcga agtaacccat aagctgatac
     9901 ccccaactca agcccaaagg atgggacatc aagggcataa actcaatatg agtatagttc
     9961 atttcaacga gataaggaat gagttcatcc ttgagctggg caaaactata aggactgcca
    10021 tcagaatttc ttttccatga tccagcgtga acttcataaa tattgacagg acgctcttca
    10081 aagccccaac gttttcttcg tgccagccaa agtccatcct tccatttctt ctcaggaagc
    10141 tctgttaaga ttgcccctgt tcctggacga gcctcatacc tgacagcaaa agggtcaatc
    10201 ttcatcagtt gatgaccatt ttgacgtgtg acatgatatt tgtaaatatg cccttcttga
    10261 gccatattgg taaagacttc ccagacccca aaatcatttc ttaccattgg aatctgattt
    10321 tcaatccagt tggtaaaatc accaaccaag tgaacagcct gagcattagg tgcccaaaca
    10381 cggaaggtat agccatgctc tccatttagt tcttccctat gtgctcctag ataatgttgg
    10441 agataaaaat tttcacccgt cataaaggtt tttaatgctt ctctattatc catatactcc
    10501 ccttctcctg taagcgtttt ctatgttttt attatactac ctttttagag aagattcaag
    10561 taaattacta tacttcttta attattttga aaatctacaa caagttcact tactcgttca
    10621 attgtaaatc aatatttttt caaaaaattg cgaaaacgc
//
LOCUS       DAVLXF010000067        10636 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLXF010000067 DAVLXF010000000
VERSION     DAVLXF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.067"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(66..1490)
                     /locus_tag="VYO12_001590"
     CDS             complement(66..1490)
                     /locus_tag="VYO12_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1588162.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVTWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2035..4314)
                     /gene="pulA"
                     /locus_tag="VYO12_001591"
     CDS             complement(2035..4314)
                     /gene="pulA"
                     /locus_tag="VYO12_001591"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1588163.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4426..6384)
                     /gene="ligA"
                     /locus_tag="VYO12_001592"
     CDS             complement(4426..6384)
                     /gene="ligA"
                     /locus_tag="VYO12_001592"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1588164.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6477..7655)
                     /locus_tag="VYO12_001593"
     CDS             complement(6477..7655)
                     /locus_tag="VYO12_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1588165.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7735..8598)
                     /locus_tag="VYO12_001594"
     CDS             complement(7735..8598)
                     /locus_tag="VYO12_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1588166.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISSHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8601..10502)
                     /locus_tag="VYO12_001595"
     CDS             complement(8601..10502)
                     /locus_tag="VYO12_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1588167.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3186 a         2352 c         1900 g         3198 t
ORIGIN      
        1 ctataattat tattatttta tagcaaaaaa ataaaaccag gaaaacaatt tcctgatttt
       61 acacgttatt tcacatcaaa aacaatggat ttgacatttg tcatcgcttc aatgctatat
      121 ttaattcctt gcactccagc tccagaacct ttgacaccaa ggaatgggaa attatctgga
      181 ccacgctggg ttttattatt aatgtggact gtacctactt caagtttttc agcaatttca
      241 aaggcttttt tgaaatcatt tgtaaagact gatgattgaa ggccgaattc agattcgttg
      301 gcaaaggcaa tagcttcctc tacactagcc acacgaatga ttggtaaaac aggaccaaat
      361 ggctcttccc atgtcacttt catatctttt gtaacttggt caaaaagcac tggccagagg
      421 agattgccct cacgtttgat tggtgtaaga gcctgagcct ctttttcttg tgcatcctca
      481 atcaagcccc aaatgaagtc ggctgaagca ttgtcaataa caggtgtaat atcagcattg
      541 tcaaatggat caccaactgt taatttagaa acttcttcct gaagcaaagt agctaattta
      601 tctgctacac tttcgagaac aatgacacgt ttaatggccg tgcaacgttg tcctgagtag
      661 ctaaaggctc ccgcaacaat ttgcttggca gcatgttcca aatctgcatc ttctagtaca
      721 agagctgcat ctttcccacc aagttccaac atgataggac gcataccagc taaacgacca
      781 atacgttctc caataggagt tgaacctgta aagttgatga agttgacttc tttgtgctca
      841 atgatataat ccccaatttc tgaaccacga cctgtaatgg tgttgaaaac acctgccgga
      901 atccctgctt cttcaaatgc tttagccaac aagagtccag aaatggaacc ttgtgttggt
      961 ggcttcaaca tgaccacatt ccctgcaatc aaggcaggtg caattttaga agcagataaa
     1021 ttaactggat aattaaaggg agcaatcgct agcacgatac caactggttc acgacggaca
     1081 acagccagtt tgtttttact tgctgcctca aaaccaccac cttccattgc ttgtccagtg
     1141 atacggagac cttcctcagc agcataacga atcaagtctg ctgtacgcac tacttctcca
     1201 attgctgctt taatcccttt tgctacttct ttggcaagga tagtaccaat tttttcctta
     1261 tcgcgttcta aaatagctgc tgttttatgc aaataagccg cacgttcaat tgctgataaa
     1321 gctcgccatg ctggcagggc tgcacgcgca gcttgcatag cctcatcagc ttcagtctga
     1381 gtcatggctg gaactgtacc caattcttct tgattgattg gtgaataaat cgtaatttct
     1441 tgttcagatg atttccattt tccatttact aaattctgat atcttgtcaa attcttgtcc
     1501 tcctgaaaat gattaagata tattttacca aattttcaga aaatcacaac cctttttgca
     1561 aaatttttga gattattttc acaatcttga tttttcaaag tatactcaat aaaaattaaa
     1621 aaaatccact acgtcaaggc gaggctaatg tggtttgaag aaattttcga agagcatgaa
     1681 tgagtatcat ctatagtaaa ataaaaaaac tgaacaattt ggttggggac agccaaacca
     1741 atttctcaca atgtttcaga aacaagggtg tgctattcca atttcagcct actataactg
     1801 tcatagattg ctgaaacaaa gtctaggtaa aagtcttcat aataaaaaga cctcctatca
     1861 agtgttcaaa aactttgata ggaggtcttg ttttgtgaaa atatttatca aattttctat
     1921 acaagtgagc tgttagccag gttctttcta ttctttcaat ttcaatgaat ggatttttta
     1981 ctaatactca taactgggaa tttgtctgtg taaaaatagc gagatagatg gtatttataa
     2041 aacactcaag acagctagac taatatcatt taaaacatta tcttcttttg agcgactgtt
     2101 ggttaccaac atagctaaat ttcctgcatt ttcaaattga tagggttctg atttagcatt
     2161 cacaactacc aagaggtgtt ctttgccgtg aacttcatag ataaggcagc cgctatgttc
     2221 aatcgcagaa tgcacaaaga catgatggta aatttcatca tagctagagt aagaaaaggc
     2281 accagttttt gtcttcaatc ggatgacttg acggataaac tcaatactgt cttgacgctc
     2341 attaatcaag ttccagttca cttggttcac actgtcagga gcattatagc tattcatcgc
     2401 acgctctcta tcatcatggg tcaactcacc attttcacca gtcgcaacca gtttggtacg
     2461 accaaattct tgaccgattt ccataaaggc catcccctgc atgagcagat tcatggctgt
     2521 ggcagtttcg accttgcgca tgatttgctc tgaactttgg tctggatgaa gggttgccaa
     2581 taaatcgtga agattgtaat tgtcatgggc ttctacatag ttaagcacct gatttggatg
     2641 tgtatagctt cctaattcac gacttcctag gattgcttta gctagaattg gctctgtcgc
     2701 agcaccactg acaaaacctg acttgatagc accataaact tctccccctt tgacagcatc
     2761 gcgctgattg tcattaaaga aaccaatatt tggcatctgg taggcattgt ccttcttggc
     2821 cttatcataa ggggcaagac ctgttcccat atcccatcct tctccataga ggataatgtt
     2881 ggagtcgatt tcatccaagc tttgacgaat catctgcatg gtcttgacat catgaatccc
     2941 catcaagtca aaacggaagc cgtcaatatt atattcctgc acccagtata gaagagaatc
     3001 aatcatatac ttgcgaaaca tttcgtgttc actggctgtt tcatttccaa cacccgttcc
     3061 attctggaag gtaccatctg gattcatacg ataatagtaa tcagggactg ttgtttggaa
     3121 tggtgcatca acaactgaga aggtatggtt atagactaca tccataatga ctccaatacc
     3181 cgcatcgtga taagcttgaa ccatcacctt caaatcacga atgacctgag ctggatcatc
     3241 tggattagtt gaaaaactag tttctggcgc gttatagttt tgtggatcat aaccccagtt
     3301 gtaggttaca tttccatcct catcgtattc tttatgacgg tctgcaattg gttgcaattg
     3361 aacataattg tagcccagct tcttgatgta atcaaaagca gttgactggc cgtattggtt
     3421 aactgttcca gcctgagcag cacccaagaa agttcctcga agatgttcat ctacacccga
     3481 tgtaggtgat ttagtcaaat cacgaatgtg catttcacag ataactgcct tacatggatt
     3541 ttccaagcgc caagtagcct ccgaaccgtg cttaacctcg aagttttcaa cttgcttttc
     3601 tacatggctc agaatagctg aacgtttgcc atcagggctg gtcgcgattg tataaggatc
     3661 acgtgtcagt gtttggtgat gagggaattg gacttgatac tgataagtct tacctaccaa
     3721 atcttcttca acatccaaac tccagacacc gattgtattg tccttatgat tataagagta
     3781 gctattgcct cttttcatct caaaagtctt ccaaacgggt gcatcattag cagctgattc
     3841 ataaacgaca acttgcactt ctgtcgctgt aggtgaccag agagaaaaat gagcctgatt
     3901 gtcctctaca cggcaaccca attctccttg gtaaccccaa tgatgatcaa aactagcact
     3961 gttaatggcc ttatcaaagg caaaaggatt ttgattttta tagaaaggac tggcaatagc
     4021 aggattttca gagtaataaa tcctatcatc gccttccaaa atccagacct ctgttaatag
     4081 gggatagtga ttaaaacgga tagaatattc tttactagtt tgacctgtat gaaccacaaa
     4141 attcaagctt tctataacat gtgaacttgg gtgttcaaag ctaaataaag ctccaaaata
     4201 atcttctttg taggttagca aatcaattcg ttgatcctga ctttttacaa aggagcaagt
     4261 gtcatattct ccattcttac gatggtaatg aatgcgcata gggtagttat acatttttta
     4321 tttttccttt ttactttgtt tctatttcac taataaattt ttgtcaatct cgtctcaatt
     4381 aacagacata gtcatattct ctaaactctg tttttaaacg atccattaca aactttctag
     4441 ccatgcctca tctctgacct ggataccaag ttcttgtgct ttttgcagtt tacttccagc
     4501 gtctgcacct accacgacga ggtcggtctt tttagaaata ctacctgtca ctttggcacc
     4561 cagactttcg agtttacttt tagcttctga gcgcttgagt cgttccaatt ttcctgtcaa
     4621 taccacggtc aaacctgaca aggccgcatc cgctactacc gtctgtcctt tatagtccag
     4681 attgacccca gtttctttca attctctgag cagaatttca gagccttctg tcgcaaaata
     4741 agtctgaaga cttttggcaa tcacgccacc tagactttca atactagcca cttcctctga
     4801 atctgcctga gacagatttt caattgaatg gaaatattga agtaaaagct gactaacctt
     4861 gcttccgaca tgacgaattc ccaaaccaaa taagagcttc tcggcagagt tttcctttga
     4921 tgcttggata gcctgataca gtttagcagc ggacttttcc ttaaccccct ctaaaaggag
     4981 gaaatcctct tcttgcaaac gataaatatc cgccacatcc ttgactaaat tagcagcaaa
     5041 aagcttctca acaatagatg gaccaaggcc tgtaatattc atagcatcac gagaagcaaa
     5101 gtgaatcaag ccttccatga tttgagcagg gcaacgcgga ttgatacaac gtagggccac
     5161 ttcatcttca aagtgcaaca agtcagagtt acaacttgga cagtttgtag ggatatctag
     5221 tttttcttca gaaacccgtt tggactctac cacacgtaaa acggcaggga tgatgtcacc
     5281 agccttatat acaatgaccg tatcgtcttt tcggatatct ttttcagcaa tataatctac
     5341 attgtgcagg gtcgcacggc taacagtcgt accggcaagt tgtactggtg ttagattagc
     5401 agttggagtt acaacaccgg tacggccaac tgtccagtca actgataaga gttgagcttc
     5461 tttttcttcg gcagggaact tgtaggctac tgcccacttt ggagccttaa cggtaaaacc
     5521 aagttcttct tgacttgcta ggtcgttgac cttgattacc actccatcaa tatcgtaagg
     5581 cagattttcc cgttcctgtc ctacttcttg gataaaattc cagatttcat ctatgttttc
     5641 agccaagatt cgcttaggat tgaccacaaa acctagttgt tctaggtact tcaaaccctt
     5701 ttcttggcta tcacgagttg aagggctggc ttcttgatag agaaacgttg caagattacg
     5761 cttggcaact actgctgtat ccaactgacg cagagttcct gctgccgcat tacgaggatt
     5821 agcaaattca ggctctccat tttcttggcg cacttggtta acttggtcaa aggaagcgcg
     5881 tggcatgtaa cattccccac gaactgtgat atctagttct tctggcaaag tcaaagggat
     5941 gtccttaaca cgcttgaggt tttctgtgat attttcacca attgaaccat ctccacgtgt
     6001 taccccagca accaaaatcc ccttttcata agtcagcgag atagataagc catcgatttt
     6061 cagctcacaa atataggtcg gatgagccac ttccttacga acacgcgcat caaaagcatc
     6121 tagctcctca cgtgaaaaag catcctgcaa actataaaga ggatactgat gactgtattt
     6181 ttcaaaacca tctaaaacct tgccaccaac acgatgagtc ggactgtctg ctagcacttg
     6241 ctctggataa gcagtttcta actcgaccaa ctcacggtaa aggcggtcat actcactgtc
     6301 tgaaaccgag ggattatcgc tggtatagta ctcagtcgca tagcgattga gcaaagcgac
     6361 taactcattc attcttttat tcataagacc attttaccat aaaacaagcc ctcctcacaa
     6421 acgagaaggg cggaaaaaac acttagtttg aaattatttt tgaaactcaa gcaaccttat
     6481 atcaattttt caaaatgagt tcgaacataa ataaacgata tacaagacaa gatgataaca
     6541 ccacttccaa ttatcaggaa agaagagaga tgtacacttg gcaagactgt cataaatcct
     6601 tttgcaatag gcataaatag aatagctaag gtaaaaattg tactcagtac tcttccaaga
     6661 aattcgctct caaccttggt ttgtacttga gtaaaaaagt gaatattaaa aatcgtcata
     6721 aacaattcac aaactaaatt tccagaaaag gaaagaaaag ttggaagtgg taatcccatc
     6781 ataaaaactc cgacacctgt caaagccagt aaaatcaaaa gattataaat attagcttta
     6841 attttactag ctagaagagc cccaatgatg gaaccaatag cccccatagt taaaatactt
     6901 gcataggctc cttctgaccc gtaaagctga ttcgaaaagg gaagtagaaa ttcaaaagct
     6961 gcaaaaaaga aattaacgct ggaagctacc agcaaaagga agaaaatttc ttgctgatgc
     7021 cagatatagt gtaacccatc cttgatatct acaaaaatat ctctcccagt aaaagccttt
     7081 ttctcttgaa cttttgcttc ctcttttgga aggaaagcca ctagaacaaa agcaatgaaa
     7141 aaagtcagcg agtctagcag tagcgtcata tggagacttg caaactgtaa aacaaggaag
     7201 gaaagaacag gagagctaac acctacaacc tgcaaaacca gctctaggcg agaattatag
     7261 atcacaatct catctttctc caccacttca gttatgatag ctttattggc tgtgcgagaa
     7321 aaggcaaaag caatagcctg cacaatgtta gcaacaatca aagcgccaat catccagcta
     7381 tcattcctta tgaaagaaat agccagacaa agaatcccac aaacaagatc tgccgtcatt
     7441 aaaatcttac gacgagaaaa acggtctgaa ataactccgc caaagggatt gacgagaata
     7501 gatgtgacga gctcagaaat ctgatacatt cctaaaactg tctgtcctat agtccccata
     7561 gaagccaacc agacactatt tccataatca tagagcatat ttcccatttt attgatagcc
     7621 ccacggctaa tcaactgcac tgcatagcga ttcatattaa agctcctctc aaattttgaa
     7681 actattgtat caaaaccgaa aggagctttt tattttttcc cttatttggg aaaattaact
     7741 tttgacaaat ttttcgtagt gttcctgata ataggctact tgctctggaa gacctaacac
     7801 atcaaaaata tgtatggcct cttgcatctg cttacagcct tctttacact gtcctttttg
     7861 atataaggca aaacctttta aataatggaa aacattacgc tcataaagct taataccttt
     7921 gtcaataatc ttctctgtat aagcctcaaa atagttggca ttataaaaag aagaatgctc
     7981 taaacaatgc tggtaacaat tgagggccaa aatcaacact aatctcttat ggctactaat
     8041 ctcttggtaa aattcctccc tctccataac ttctctacca atccgagtga cataatccac
     8101 atcgtagaaa ctatagaggt taccgaaaag aatcaactca tacatggtcc attcttctgt
     8161 tttgaagaga taatctgcta ccttatccaa atcatcctgc ttcatatcat aactcgcatc
     8221 tctttgacaa atcagaccct gtagcaaaat ccagttcagc tcaaaataaa ggggagtcgt
     8281 cgaactctta gacttttcaa gttgttctct ttgaagcttt tgaaaacctg caatatcgtt
     8341 tgaatagtaa agtgggataa tctgtgccat catagacaca tgttcatgat tatgaaaatt
     8401 ccttgcctta tccatgaaat tttcgattgt tacatgaatg ttatccaaaa tctcaaagaa
     8461 acgggagact gccaggtcag actccccaag ctcaaagcga gataactgag aggtagagca
     8521 ggattcgcct gctgcttcct tcaaagaata atttccactt gttcgaaatt cacgaaatac
     8581 ttttccaaga tgttccatct ttacacctgc tctgataatt cttcccactc aagcatagct
     8641 tcttcctgac gatggctgat tttgtccagc tcagcctgta attccatgag tttgtcggca
     8701 tcgtttgttt ccaacatttg ttcagaaatg gcttggcttt gactttctag ctcttcaatt
     8761 tcagcttcta gactttcgat ttgtcgcatg agtttgcgaa cttctttttg actttctttc
     8821 tgggcctgat agtcattgac tggacttgct tcctttgctt gattgctagt tgaagcttcc
     8881 tcagtctgac tcatttctgc tgttgctttc ttctcaacat agtagtcgta atctccaagg
     8941 tagagagttg aaccattctc agacaattcc aaaacatgag ttgccacacg attgataaag
     9001 taacgatcat gactgacaaa aagcaaggta ccatcaaagt caatcaaggc attttctagc
     9061 acttccttac tatcaatatc caagtgattg gtcggctcat ccagaatcaa aaagttattg
     9121 ttttccatag acaatttagc taaaagcaaa cgagcttttt cgccaccaga tagcatgccg
     9181 actgattttt taacatcatc tcctgagaaa aggaaggctc caagacggtt gcggatttca
     9241 acttctggtg tcaatttgaa atcattccag agttcatcca gcaccgtatt acttggtgtc
     9301 agcttgcttt gggtttggtc atagtaacca acctcaacat tagcgccaaa gcgcttttct
     9361 cccttgataa acggaatttg atccacgatt gatttgataa aggttgattt tccgatacca
     9421 ttgggaccaa cgatagcaac agcattcatc ttacgaaggt ctaggttaat cggttgtgac
     9481 aggacttccc catcatagcc aacagccgca ttttcaacag tcaaaacaac attgcccgac
     9541 gttttttcag actggaaggt catgttggct gctttcttgc cagcttccgg cttgtccaaa
     9601 cgctccattt tttctagttg tttacggcga gattgagcac gtttagtcgt tgaagcacga
     9661 actagattgc gattgacaaa gtcttccaga gcagcgattt ccttctgttg cttttcatag
     9721 ttttttgcct cagtagctag cttttgctcc ttcaactcga caaaacgaga gtaattcccc
     9781 acatagcgat ccaaggaatg cttggtcaaa tctagcgtaa ttgtcgcaac cttgtccaag
     9841 aaataacggt cgtggctgac gataatgagg gcaccgctat agtttaccaa gtaattctct
     9901 agccaggaga tggtttcaat atccaagtgg ttagttggct cgtccaagac caagagattg
     9961 ggcttttcaa ggagcatttt agctagagcc aaacgagtat tttgaccacc tgaaagttca
    10021 gctattttca tctgccacat agactcgtca aacttgaatc cattcaaaat cgctcgaata
    10081 tcagcttcat aggtaaagcc acctgcttgg cgaaaattct cagataagcg gtcataatct
    10141 gacatcagtt tatccaaatc ctcaccagac ttttcaccca tctccagctc catctgacgc
    10201 agttgtctct ccgtccgacg caaatcatta aagacatgaa gcatttcatc gtagatggta
    10261 ttttcagact caaaacggct atcttgggct aggtaagaca gagaaatatc ttttttctta
    10321 ttgatttctc cgctagttgg ctcctcttct ccaactaaaa tcttcaaaag agtagactta
    10381 cctgcaccat ttttcccaac aagagcaatc cgatctcgtt catcaacctg caggttgata
    10441 ttatcgaaaa gaacctctcc tgcaaaagaa cgttcaattt tattagcttg taaaataatc
    10501 ataagagtag tatagcatgt ttccctaagg cattcaagat aatcgtaagt cttttagtac
    10561 aacttttata acataaaata aactaaatta tgtatatttt atattagatt ccttcactat
    10621 cttgttggat tttcta
//
LOCUS       DAVLXF010000068        10488 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLXF010000068 DAVLXF010000000
VERSION     DAVLXF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.068"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            28..381
                     /locus_tag="VYO12_001596"
     CDS             28..381
                     /locus_tag="VYO12_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1588168.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            596..712
                     /locus_tag="VYO12_001597"
                     /pseudo
     CDS             596..712
                     /locus_tag="VYO12_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            705..938
                     /locus_tag="VYO12_001598"
     CDS             705..938
                     /locus_tag="VYO12_001598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588169.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            1066..1208
                     /locus_tag="VYO12_001599"
                     /pseudo
     CDS             1066..1208
                     /locus_tag="VYO12_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1345..2313)
                     /locus_tag="VYO12_001600"
     CDS             complement(1345..2313)
                     /locus_tag="VYO12_001600"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1588170.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2458..3081)
                     /locus_tag="VYO12_001601"
     CDS             complement(2458..3081)
                     /locus_tag="VYO12_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV1588171.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSPFLNA
                     FESAIVAPLVEEPLKLLSLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(3063..3272)
                     /locus_tag="VYO12_001602"
     CDS             complement(3063..3272)
                     /locus_tag="VYO12_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588172.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKNGNCQRRF"
     gene            complement(3297..3794)
                     /locus_tag="VYO12_001603"
     CDS             complement(3297..3794)
                     /locus_tag="VYO12_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1588173.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(3867..5228)
                     /gene="radA"
                     /locus_tag="VYO12_001604"
     CDS             complement(3867..5228)
                     /gene="radA"
                     /locus_tag="VYO12_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1588174.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(5242..5757)
                     /locus_tag="VYO12_001605"
     CDS             complement(5242..5757)
                     /locus_tag="VYO12_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1588175.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(5759..6202)
                     /locus_tag="VYO12_001606"
     CDS             complement(5759..6202)
                     /locus_tag="VYO12_001606"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1588176.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(6259..6356)
                     /gene="ffs"
                     /locus_tag="VYO12_001607"
     ncRNA           complement(6259..6356)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYO12_001607"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6389..6856)
                     /locus_tag="VYO12_001608"
     CDS             complement(6389..6856)
                     /locus_tag="VYO12_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1588177.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            7137..7286
                     /locus_tag="VYO12_001609"
     CDS             7137..7286
                     /locus_tag="VYO12_001609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588178.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            7428..7607
                     /locus_tag="VYO12_001610"
     CDS             7428..7607
                     /locus_tag="VYO12_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588179.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(7745..8701)
                     /gene="lytA"
                     /locus_tag="VYO12_001611"
     CDS             complement(7745..8701)
                     /gene="lytA"
                     /locus_tag="VYO12_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1588180.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLAD
                     RWKKHTDGNWYYFDQSGEMATGWKKIADKWYYFDVEGAMQTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTMADKPEFTVEPDGLITVK"
     gene            complement(8705..9037)
                     /locus_tag="VYO12_001612"
     CDS             complement(8705..9037)
                     /locus_tag="VYO12_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1588181.1"
                     /translation="MQQITEIITNGAISILVILAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVASETGFKGDEKLEQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            complement(9041..9457)
                     /locus_tag="VYO12_001613"
     CDS             complement(9041..9457)
                     /locus_tag="VYO12_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV1588182.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            complement(9467..9817)
                     /locus_tag="VYO12_001614"
     CDS             complement(9467..9817)
                     /locus_tag="VYO12_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588183.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTGEPKE"
     gene            complement(9820..10023)
                     /locus_tag="VYO12_001615"
     CDS             complement(9820..10023)
                     /locus_tag="VYO12_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588184.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITSNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(10117..>10488)
                     /locus_tag="VYO12_001616"
     CDS             complement(10117..>10488)
                     /locus_tag="VYO12_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV1588185.1"
                     /translation="WNPKGGRNAVVWWNQVGSGSVKYWMEQKSDRRLKENITDTAVKA
                     LDKINRLRMVAFDFIENKKHEEIGLIAQEAETIVPRIVSRDPENPDGYLHIDYTALVP
                     YLIKAIQELNQKIEKMEKTIA"
BASE COUNT         3116 a         2461 c         1897 g         3014 t
ORIGIN      
        1 tcaattcact atagaaggag gaaaaggatg attctcagac atctgggaat tagcccgacc
       61 aatgacttgg ttgctaagaa aattttcagc aacccagaaa tcacttgtca atttatccgc
      121 gatatgctgg acttgccagc caaaaatgtg actattttgg agggaagtaa cattcatgtc
      181 ttgccttcca tgccctactc agcgcaggat ttttatacca gtatagacgt cttggcggag
      241 ttggataacg gtactcaagt aattattgag attcaagtcc atcatcagaa ttttttcatc
      301 aatcacttgt gggcttacct gtgcagtcag gttaatcaaa atcttgaaaa aattcgtcag
      361 cgagaaggtg atactcacta gatctacaaa cacatcgctc ctgtttacgt cattgctatc
      421 gtggatagta attatttctc agatgacctg gcttttcata gctttagtat gcgcgaagac
      481 acaacaggtg aggtattggc gattaccaac aatggacagg aaaaccatct ggttaagatg
      541 gcattcttgg aattaaaaaa tacagagaaa ccagcaaaga caaggttcgc aagccatggt
      601 tggagttttt cggcaacaag ccctttaccc agcaaccgca acgagccatt agccaagcaa
      661 atcaactgct ggactacaag agctggtccg aggaggacag gaaaatgttt agtcaactac
      721 atatgcgaga agaacaagtc ttgttagcac aggactatgc cttggaaact gctagggctg
      781 aaggccttga acaaggacta gagcgtggga aagttgaagg aagggcagaa aggaaacttt
      841 ttgccttcct agacatagta cgccaaggtc ttctgacttc tgaggttgcc agccagcaat
      901 taggtatgtc agtatctgaa tttgaggcac tgttgtaaaa tggctccata atatccatag
      961 tgggtaaatc ccctatggat attatggagc ctattttgtg taaaaaaaaa agtcccatat
     1021 gacctataat gaaaagcgac aaaacaactc attagaaaga atcatatgga acaattacat
     1081 tttatcacaa aattactaga cattaaagac cctaatgtcc agattttaaa catcatcaat
     1141 aaggatacac acaaggaaag cgtcgattca aatgctatca ctgttcaaaa atgatggtcg
     1201 ctgaaactta tgatgacgta cagtcatatt tcttttcttt ttattatatc acagttttaa
     1261 atctagcttt actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc
     1321 tgagaatcat ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag
     1381 tggtcgtttt tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg
     1441 ctcaatctta tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt
     1501 cttaatagct gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca
     1561 gcttgcatag acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt
     1621 tccagcggta tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat
     1681 gatgttcata acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc
     1741 gatagatgtt ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg
     1801 gctgacaacc acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag
     1861 aggagcaccc atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg
     1921 caagtcgatg gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt
     1981 tgaagtgatt ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg
     2041 catgacaaca ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat
     2101 ttcaagcaga ttgtcattta caggcgaact agttgattgt aagataaaga cgtgtttccc
     2161 acggattgat tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga
     2221 tttccccaac tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga
     2281 aagggcaaac agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatagct
     2341 tgtgcttttc acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc
     2401 aaaaataaaa gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta
     2461 ggtcatcaac tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc
     2521 aatagccgta accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga
     2581 gttaaacaag aagtgagttc ccaaggctaa acctagaaaa ataaggccct gtttcttgcc
     2641 aaccttctgt cctttatagg ctctgtaaag caagtaaaca cctactacag ctagacctga
     2701 aaaagtccag tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa
     2761 gtcaaagccc tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc
     2821 caaaccggaa gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc
     2881 caaaacaaaa acaagtgaca ataatttcaa gggttcttct accaaaggag ccacaatagc
     2941 actttcaaag gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat
     3001 ataagtatta gcaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag
     3061 aatcaaaacc ttctttggca attcccattt ttcccaatac ggaagagaaa ataaagagcc
     3121 ggaatcatgt aaaagagagc tagaaagata gaaactccca ttagtccata ttccgcacct
     3181 gacctcgaac cgtccgtata gtagatggtt tcatactgta aaccaataca tagcaataaa
     3241 ataaaaataa ataaaatact gcttttcttc atacactttc tttctaaatg aagtatttat
     3301 aattctacga ctgtcatact tcctgtatca acattgtaaa tggcaccaga gataatgaca
     3361 tcgtctggta ttaggggaga ctcgataagc agttgcatat cctcgcgtac actctcttct
     3421 atatcttgga agggcaagaa gtcctggtct gacacatcta cacctaattc ctcttttaaa
     3481 tactcctgaa aaggttcatt ttcaaaggtc tgagcaccac agtctgtatg gtgcaatacc
     3541 acaatctctc ttgtccccat ttgttgctgg gaaataacta gcgaacgaat catgtcttca
     3601 gtcactcgac cacctgcatt ccgcaagata tgagcatccc caagtgccaa gcccagagct
     3661 tgcgcaacgt gcagacgaga gtccatacag gtcacaatag ctactcttgt tttgggttta
     3721 agtggcagat ttaactgccc atgtagggca acataagcct gattggcttg cataaactgt
     3781 tcaaaatacg acacgattcc ctccttgaaa atttgatagt caaatatttc tcctatctta
     3841 tcatttttaa gagaatttgt cacggattat gcaaagacct ttttcaagac ttcctgaatc
     3901 gttgtcacgc caatgacctg aatttcctta ggcagagtga ttcctgtcaa ggaattctta
     3961 ggtacataaa tcttagtaaa gcccagttta gcagcttcgt tgatgcgttg ctcaatacga
     4021 ttcacgcgcc gaatctctcc tgtcaagccc agttctccga caaaacattc ctgaggatta
     4081 gttggcttgt ctttgtagct cgaagcaata gcaactgcaa cagccaagtc aatcgcaggt
     4141 tcatccaatt taacaccacc agcagatttg agataggcat cctgattttg caagagaagc
     4201 cctgcccgtt tttccaaaac agccataatc aagctagcac ggttaaaatc aagtcctgtc
     4261 gtagtacgct tggcatttcc aaacatggtc ggtgttacca aagcctgaac ctccgccaaa
     4321 atcggacgcg tcccttccat ggttacaacg atggaggaac cagtcgcccc atccaaacgc
     4381 tcttctagga aaacttgact cggattgagt acctcaacca agccgcccga ctgcatctca
     4441 aaaatcccaa tctcattagt ggaaccaaaa cgatttttga ccgctctcaa aatacgaaag
     4501 gtgtggtgac gctccccttc aaagtaaagc accgtatcca ccatatgctc caacatacga
     4561 ggcccagcca aggttccttc tttggtcaca tgacctacga taaagatggc aatgttattg
     4621 gtcttggcca actgcatgag ttcagcggtc acttcacgca cctgagaaac agacccctgc
     4681 acccctgaaa tctcaggaga catgatggtc tggatggaat caataatgag aaagtctggc
     4741 tggatacgct ccacttctgc acgaacactc tgcatattgg tctctgcata gagataaaac
     4801 tcactatcaa tatcacctaa gcgctctgca cgtagtttaa tctgctgggc agactcctcc
     4861 ccactgacat agagaactgt ccccacttgg gacaactggg ttgagacttg taggagaaga
     4921 gttgatttcc caatcccagg atccccaccg atgaggacga gacttcctgg taccactccg
     4981 cctccaagca cacggttgaa ttcctccatc tccgtcttgg ttcgattgac attgatggaa
     5041 gtcacctcag ctagtttcat gggcttggtt ttctcacctg tcaaggacac acgcgcattc
     5101 ttgacctcgg caacctcaac ctcttccaca aaagaagacc aagacccaca gttggggcaa
     5161 cgtcccagat atttagggga attataccca caattttgac atacaaatgt cgcttttttc
     5221 tttgcgatga caaacctctt tctatatctc taactcacac tcaatcactt ggcaaaaatc
     5281 aatcttctca tttggcacaa actggcgcat gagcattcga tgagcaacaa ctaccacagt
     5341 ctgatgttct cgatacttag acatacattc tagaaaccga gacttcattt ccgtagctgt
     5401 ctcatattga ataggactat taggaagcaa ctcccccttg ttttctaaaa acagtcttct
     5461 agctgtttca aaattttcta ttcctgtttt atagacctgc cattcatgta ataaaggctc
     5521 tactcttaaa ggaagacccg tagcacagac cacatacgaa gccgtttcta aagctcttgt
     5581 gactgcagaa gatacgatta tttcagctga cgagagtaaa ggatttttgc tcaatttctg
     5641 gacttgctgc cgtcccatct cagacaaggg tgccaaatct atcccaaatc ctatataaga
     5701 acgctcctct aactcacggt aatctggctc cccatgacgt acaaagataa tcttcattct
     5761 agtgccctgt cgatccaaat ccaccagttc gaacgccatc agctgcatct ccatctgcaa
     5821 ttaagaaagt agcaaaaaca gcctggacaa tacgttcccc aacttcaaga acaacctctt
     5881 gatctgtgat attcttcatc tgcgcaaaaa tatgcccttc atttccagga tttccataat
     5941 aatccccatc aatgacccca actgagttaa ttaaaaccaa gcccttctta cgaggatttg
     6001 aagaacgatc atagaggtag agaacctcag tcggctgcat ataagcctta acccctgtcg
     6061 gaaccaagac aatctctcct ggcgcaacaa ctgtacgcac agcaaccttt aagtcgtaac
     6121 cagccgcatg cgctgtctca cgcttgggca ataaattttc atctgtaaaa ctcgaaacca
     6181 attcaaaacc acgaattttc ataattttct cttttctatt atcatttatt ctagattatt
     6241 ctatcttatt tattcggaaa aagcacgaaa aaaagagcac acaattcaaa tcgcttaggg
     6301 ctgctggatt cctcccctga cccgcttcac gcagaactgt tgctccacta tttattatat
     6361 cacattccta ttcattttaa aagcaaaatt attttttccg tctatttcta aaaaaatcct
     6421 gcatgatagc tgcgcattca tcttccaaaa ttcctatttc aacctccaca cgatgattga
     6481 gacgctcatc tgtcaagata ttgtacaaac tcccagcagc gccaaatttc tggtttttag
     6541 ccccatagac cacatttgga atgcgggcaa gcccaatcgc tccactacac atgacacaag
     6601 gttcaatggt cacaaaaagt gtgcaatcca gcaagcgcca gctctcctca ctcaagttcg
     6661 catcctctat agccataatt tccgcatgca taaccgctcg ctgtaattcc tcacgcgcat
     6721 tatgcccacg accaatgatt tccccatctt tgacaatcac acaaccaatt ggaatttcat
     6781 cgtgttcaag agcaatctca gcctctctca aagcctccct cataaagact tctttttctt
     6841 caagcgtata atacatctct tttctctttt cctacttatc gattttatta ttatatcatg
     6901 aatcccaaca caaaaaaagc caccgaatgc ggtgactcta tagggagatt attatgaaaa
     6961 aggtaaaata aaaccttatt aaatcaatgc ttttggaggg tgccccctcc aactccccga
     7021 cctctggaca aggtctattt ttttttgaaa aaattaaaaa aacttcatca aaacgcttga
     7081 ctttctcggt gtaccgtgat ataatataat caagataagg aaagggggtg atgaagttga
     7141 acaaagaaga ttggctcagg ttacttgaaa aggcaataga caatattcct gaaacggtaa
     7201 ctgctatcgc aagtctagtg accgcaataa cggtcgcaag gcaaaacaaa aagcgtaaac
     7261 cgaaatcccg tcaaagaaaa aggtaaacgc taagaggttg gggcgaaagc cccttacacc
     7321 tctattttat caaatggaaa gaggaaatgc aatggttagt gcaatagcta tttttataat
     7381 tgcaatcaat gtatatattt atctaaaaaa taaaaaggac aaataagatg agagaagtta
     7441 ttcaagaatt gttagacagt tcgatgtcta catctgctat ttcccaaggt gctggagttc
     7501 catggactac tgtttctgac ctcagaaaag gaaaaacaag catggacaaa atggcacttc
     7561 tcacagcaga aaaactttat gaatttgcta caactgataa gcagtgattt cggtcactgt
     7621 ttttattttt gacaaaacac ctccctattc ttcaagtaag cacttttttg aaaaatcaga
     7681 gcaaataaaa aaccgcaagc ctgagcctgc ggtgaaagaa caatttagaa agtttccttt
     7741 ctatttattt aactgtaatc aagccatctg gctctactgt gaactctggt ttatctgcca
     7801 tcgttccgtc tggtttgagg tagtaccagc ctgttccgtc cgctgactgg ataaaggcat
     7861 ttgataccat agcaccttct ttagcgtcta agtagtacca agtgtccttg tacttgaccc
     7921 aacctgtttg catagcacct tctacgtcga aatagtacca cttgtcagcg attttcttcc
     7981 agcctgtggc catttcgcct gattggtcaa agtagtacca attaccgtct gtgtgcttct
     8041 tccagcggtc tgcaagcata tagcctgagc catcgaaata ataccaggta ccgttgattt
     8101 tctcaaactt atcttttgga taagagccgt ctgagtgtac gtaccagtag ccagtgccat
     8161 ttttctgcca gcctgttgca gcgctcaagc cgttttcgat gtcttgctta aactgttcac
     8221 ggctaatgcc ccatttagca agatatggat atggatccac atggtctgag tggttgtttg
     8281 gttggttatt ggtacagtat tcatgcgtct tgatacctgc caagtcgtct gtatcaagag
     8341 tcttcggcaa gcctgcttcg tccgctagat tgcgtagcaa ttcgatataa aggcgatagt
     8401 cagccataaa ctcttcctta gttgaatggc tttcaatcag ttcaaccgct gcataggtct
     8461 cagcattcca accgccccca acatcccaac ttccgttgtt cacaggtcct acctgcatga
     8521 tgcgaccgtt cccaacaacg tgcgagaaaa aacctaattc tgggtctttc cgccagtgat
     8581 aatccgcttc attctgtacg gttgagttgc ggttacctgt tgagtgagca tgtacttgtc
     8641 gataaggctg caccccaacc tgtggcaagc ctgtacgtag tctgtttcta tcgatatcca
     8701 ttccctatcg tcctttccat gcgtcattca tctgcttcac tgctgactct acgaaggtgt
     8761 ctaagtcttt gtcagtcata ctaatattgt attttgtaag ctcagcacgg actttagcac
     8821 gagcctgctc cagcttttca tcgcctttaa agccagtctc tgaggcaacc tgctccacgg
     8881 cattaactgc gtttttggcc aagatttcaa cgattttgat ggtcttttct ccaccttttt
     8941 gaaccaggta gtccttgact gccctaacta ccacccctgc taaaatgact aggatgctga
     9001 ttgctccatt agtaatgatt tcagtaattt gttgcatttg ttattctcct tttttcgtat
     9061 catcatcttt ttcaagtaat cgctgaaata cttttacaat cggctgaaaa agagtaacat
     9121 ttccttttaa tttgcggtaa ttttcaatga gtgattgaaa agtaaatgcg atgtacccga
     9181 gatagattga gtacaagaat gcaaaacctg tcttttcagg caacaaaacg gacgccggaa
     9241 tgaggatcat cagtaagaga acccctgaaa tcttacgaag gagcccgtta atgccgattt
     9301 tgctcttgta ctcgatgtca ggattgataa tcgccgcaat cgtccctgtc acaaaatcaa
     9361 tgatttccat tgagacaatc agtgctagag cgtacaagac caaaccatct tcagtctgta
     9421 cgacacttct tagaaaattg aaaaattcaa tttgcataca catctcctat tctttaggtt
     9481 ctcccgttgg atccgtccag tcaggattgc cctctgcatc aaatttcatg atatagaatt
     9541 catgattcaa cagaacggcg acgttgattg ttgcgattgt accaccccac tggttgaacg
     9601 cccaaacggt ttcaacatcc ttgagttggc gacggccatt tacgatcaca ggacgttttt
     9661 gaacatcacg atacatatag aagtcatcgc ttacattctt gcaacgaatg aactctccat
     9721 tttctttcat atagcgcaaa gcgctcgcaa gatcaaatgg ttctgtgatt tttgtaaggt
     9781 ctagcaagtt atctgtgttt tgaattgttt ctgccatgtc tattctcctt tgtctgctgg
     9841 tttagtttgt tcatcaagca gagcttccag ctcatccact cgtgcttgaa gtctttgatt
     9901 ctcttccctt tgctcatcca actgaatact caagatatta cttgtaatca tcgaatttgt
     9961 tgaagttgtt gacatttcac taattgtcat ttgtaaggct tggttaagct gttctgtgtt
    10021 cattttctaa gttctccaat ctgtgtgttc gttttctatt ttcaagagca agctcctgaa
    10081 ttgctttaag tgcgatattg gtcaacatgt tgttattcat gctattgttt tttccatttt
    10141 ttctattttt tgatttaatt cttgaatagc cttgattaag taaggaacta aagcggtata
    10201 gtcgatatgt agatagccat ctggattctc aggatctcgt gagacaattc ttggaacgat
    10261 ggtttcagcc tcttgagcta ttagaccaat ctcctcatgt ttcttatttt cgatgaaatc
    10321 aaatgcaacc attcttaatc tgttgatttt atccaaggct ttcacagctg tatctgtgat
    10381 gttctctttt aagcgtctgt ctgatttttg ttccatccaa tacttcacgc taccgctacc
    10441 gacctgattc caccaaacaa ccgcattcct tcctcctttg ggattcca
//
LOCUS       DAVLXF010000069        10470 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLXF010000069 DAVLXF010000000
VERSION     DAVLXF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.069"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(385..777)
                     /gene="rpsI"
                     /locus_tag="VYO12_001617"
     CDS             complement(385..777)
                     /gene="rpsI"
                     /locus_tag="VYO12_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1588186.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(797..1243)
                     /gene="rplM"
                     /locus_tag="VYO12_001618"
     CDS             complement(797..1243)
                     /gene="rplM"
                     /locus_tag="VYO12_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1588187.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1599..1916
                     /locus_tag="VYO12_001619"
     CDS             1599..1916
                     /locus_tag="VYO12_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1588188.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(2066..2878)
                     /gene="folK"
                     /locus_tag="VYO12_001620"
     CDS             complement(2066..2878)
                     /gene="folK"
                     /locus_tag="VYO12_001620"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1588189.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2921..3475)
                     /gene="folE"
                     /locus_tag="VYO12_001621"
     CDS             complement(2921..3475)
                     /gene="folE"
                     /locus_tag="VYO12_001621"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1588190.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3456..4778)
                     /locus_tag="VYO12_001622"
     CDS             complement(3456..4778)
                     /locus_tag="VYO12_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1588191.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDSPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4780..5754)
                     /gene="folP"
                     /locus_tag="VYO12_001623"
     CDS             complement(4780..5754)
                     /gene="folP"
                     /locus_tag="VYO12_001623"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1588192.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFNQTFTVEELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5849..6556)
                     /locus_tag="VYO12_001624"
     CDS             complement(5849..6556)
                     /locus_tag="VYO12_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1588193.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6609..8027)
                     /locus_tag="VYO12_001625"
     CDS             complement(6609..8027)
                     /locus_tag="VYO12_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1588194.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            8217..9029
                     /locus_tag="VYO12_001626"
     CDS             8217..9029
                     /locus_tag="VYO12_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1588195.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9368..10387
                     /gene="adhP"
                     /locus_tag="VYO12_001627"
     CDS             9368..10387
                     /gene="adhP"
                     /locus_tag="VYO12_001627"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1588196.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKGKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3097 a         2354 c         2037 g         2982 t
ORIGIN      
        1 cttccccatt ctatggaatc ttgcattatc cataatatag tagattgaaa ctagaatagt
       61 acacctctac ttctaaaaca ttgttagaat tcgatttgac tatcctcgat ttgtcctatt
      121 cttatttcat attactataa aaataaggta aacttttcaa aaaagcacga aaaaagcacc
      181 cataaggtaa acttttgagt gctttgaaac attgatataa tcgtattgat taacgttttg
      241 aggcaactga ccttgtcagg ttgcagccac atttgtaagc gactaaagtc gcaacaactg
      301 tgtcaattgc accaatttag taagaaagta taaaaaaaga acaccccgaa aggtgctctg
      361 tataagtata gtaattcttt cgaattaacg tttactaaat tgtgatgctt tacgagcttt
      421 cttaagacct ggtttcttac gttcaacttt acgtgagtca cgtgtaagaa gtcctgcgcg
      481 tttcaatgaa tcgcggaagt ctgggtctac ttgaagaagg gcacgagcga taccgtgacg
      541 gatagctcct gattgaccag cgtatccacc acctacaacg ttaacgaaaa cgtcgtatga
      601 acctacagtt gaagtaactg cgaatggttg gttgatgaca agacgaaggt cagcgtgtgg
      661 gatgtactct tcaacatctt ttttgttaac agtgatttta ccagttcctg gaacaaggcg
      721 aacgcgtgca acagcgtttt tacgacgtcc agtacctgca tattgtgctt gtgacatact
      781 ttatttgttc ctttccttag ataagtcctg aaatgtcaag aacttctggt tgttgtgcag
      841 catgagtgtg ctcagctcca acaaatactt tcaacttcat accttgagcg cgtccaagtg
      901 tattgtgtgg aagcatacct ttaactgatt tctcaatcaa acgtactgca tttttagaac
      961 gaagttcacc tgcagagatt tgtttcaatc cacctgggtg gtttgagtga gtgtagtaga
     1021 ttttatcagt tgctttttta ccagtcaatt taactttttc agcattgata acaatcacga
     1081 agtcacctgt atcagtgtgt ggtgtaaatg ttggtttgtt ttttccgcga agtacgctag
     1141 caactactgc agaaagacgt ccaagtggta catcagttgc gtcaactacg taccatttac
     1201 gttcaacttg gcctggttta gccataaatg ttgttttgtt catgatttct cctatatata
     1261 gttcgatttt tgtttacggt gatgggtgtt ccttcccatc gaaaagtatt tggaaggttc
     1321 cggggccttt caaatggggt aaacaatacc gcctactatc ataccaaatt tcacccctaa
     1381 aagtcaatag atatgaaaaa tatttttcgg taacttctca aagtaactga aagttatata
     1441 atcaagttaa tttgggaatt tacctattaa cttcggtaga tttttaggaa ttttttccct
     1501 atttctccct gtaactaaaa tccttttcaa aaaatcacct tttcttgatt tttaatccta
     1561 tttgctgtat gataagggaa aagaaagggg acagagatat ggcttttacc aatacccaca
     1621 tgcgatctgc tagttttggc attgttacca gcttgcctga tgacatcatt gactcttttt
     1681 ggtatatcat cgaccatttc ttaaaaaatg tctttgaatt ggaagaagaa ctcgagtttc
     1741 aattgcttaa taaccaagga aagattacct tccacttttc aagtcaacac ctccctacag
     1801 ccattgattt tgactttaac catcctttcg accctcgtta tcccccaaga gtactggttt
     1861 tagacatgga cggtagagaa actatcctcc tcccagaaga aaatgaccta ttttaatact
     1921 caatgaaaat caaagagcaa actaggaagc tagccgcaag ctgtacttga gtacggtaag
     1981 gcgacgctga cgtggtttga agagattttc gaagagtata aaaactctag ccttcagttg
     2041 caagtaactg aaaactagag tttttctatt ttttcaaagc atcatacaag ttgcggatcg
     2101 gttgttttaa tatcggatgg ataaaatgag gcgcaatttc ctgtaaggac tcaaggacaa
     2161 aaaggcgttc cgctatgtaa ggatgaggca atatgaggtc gtctgtataa aggatctggt
     2221 cctccacaaa gagcaagtcc aaatcaatca aacgaggtcc ccaatgcact tctctcaccc
     2281 gtcccagctc tgactcaatg gctaacaagg tttctaacaa gtcttgtgct ggtagccagg
     2341 tttccacctc aaccacttga ttggcaaagc tatcctgctc cactccaccc caaggctccg
     2401 tcgctaagac actggactct ttgagaatat ggatgccacg agctcgcagt ttgtcaatgg
     2461 cttgcttcaa gtttacttgt ttatctccca tattgcttcc tagggcgata aaggctcgtt
     2521 gcttgcggcg atgaatggtt accgagcaag tatctagtga caaatgcacc ggtgcccaag
     2581 gttttttcag ttccaacttc atttcttgga caagaggata agactcaaag gtacgttcca
     2641 ccagtttata ggctaccgtt tcaatcaaat cttcactcgt ttcctgaaac caagtcgtcc
     2701 actgctgaca caattctccg taatggacag aggctgttaa atccaagtct gtagctgcct
     2761 tggtcatatc ataggatagg atggctgaaa cgacaaattt ctgccccaat tctttctcac
     2821 taggaaaaag accatgatag gcaaacattt ccaaatcctt aatctgcagt tgatccataa
     2881 tgattccttt ctagaaaaat ccgcttgaag cggattcttt ttatagcccc attaaacgat
     2941 aagcttggtc gcggaggtcc ttatctgttt caaatagacc acgagctact gtcgtcaagg
     3001 ttgcagtgcc tggttttcta acaccacgca tactcataca catatgttcc gcctcaatga
     3061 caacaaaggc tcctttagca cctagatagt ccatcaaggc atcggccact tcgatattca
     3121 aacgttcttg aatttgtggt tttttcgaat aaacttcaac cgtacgggct agcttagaca
     3181 agcctgccac acgaccatct ggaatgtagg caatgtgcgc tctaccataa aatggcaaga
     3241 agtggtgttc acacatggta tggaaaaaga tatccttttc taccaccata ttatcgtcaa
     3301 taatttcaaa ggattttgac aaatgttcct ctgctgtttg accaagacct gaaaaaatct
     3361 cttgatacat acgggctaca cgagcaggtg tttcctgcaa accctcgcga ttagcgtcct
     3421 ctcctacagc ctcgataatc atttttacag ctgcttcaat cttttgtgta tccattctcg
     3481 ttcttcctct ccatcagata ggccctcact tggctcaaga aatacaagga acctgtgaca
     3541 atcctaactg tttgtttctc ttctttttta tctgtcaaat tctgctctag aaaatcatgc
     3601 caatcttggt agctgagatt tctagactta gctgcctctt tcagcacgct ttcatccgtc
     3661 gcccgactat ccgcaaaatg tgttagagta agctcggtat ctggcatggc tcccagcaag
     3721 tccaacatat cctccaaggc cttggttttg atacaagtga agaggatttc cttatgataa
     3781 tccgcaaaac gttcttgcaa ggttaccaac aaggccttga tagcatgggg attgtgggct
     3841 ccatccaaaa tcatcaaggg atctcttgac acgatttcca aacgccctgg ccaacttgtt
     3901 tcttccaagg cttgaccaag aaagtcattg cttgctagct ctcgaccatc ttcttgacaa
     3961 aaagtatcaa gtaaagctat ggccatccca gcattctcta tttggtacaa accaagcagg
     4021 ctagtctgga agcgaccttg tctgacagca cttgtatagt caaagacttc ccctgtcacc
     4081 acactttctt gatgacgaac ctgataatct gtcccgtagg caagtctcgg cgaatctttc
     4141 ccttccgcaa tgcggtcaat cacagtcaag gcttctggag caatacgccc tgttaccaag
     4201 ggcatccctt gtttgataat acctgccttc tgctctgcta tggcctccaa ggtgtcacca
     4261 agtagggcca catgatccaa gccaatagtt gtaattcctg tcaaaatggg ctgacagaca
     4321 ttggtactat ccaaaagtcc acccatgcca acttccatga tggccacatc tacttgctct
     4381 gaggcaaagt agtcataggc cagggctgtg ataatctcaa actcggttgt gccctgtaaa
     4441 ttggcgaccg cttctccctc cagcaaagac tgatagtctg ccatgagagc ttctagcctc
     4501 gcttctgaga tcgattcccc attgatgcta atctggtctg tgtaatgaat gagatagggc
     4561 gagctaaaca cgccaactct cagccctagc ttttctagca tctttttcaa aaaagcaata
     4621 gtcgagccct tgccgttagt ccctccgata tggaggacct tgagtttgag atggggattg
     4681 ccacgcaaag ctaacagttc caccattcgt tccaagccaa aatgcggttg atccgtccgg
     4741 tagttagcaa tccactgatt gttttcaatt tctttcatct tatttatatt gctttaaatc
     4801 taaattttcc gcttcatcag ccagacgaat ggcagatgca atttcaactg ccatcctgtg
     4861 actagctacg tcatgcacgc gcaccacttc tacaccctgt ctcgcagcga tactagttac
     4921 atgagccgaa gccgtgtccc gatttcggaa accaagctct gtctcaggat tgacttcaaa
     4981 cccactttct tcaaggatat tgatgacaaa tcgcttgcgc gacactccga gaaagattgg
     5041 atagcccttc tgatgtagtt tatccagatc ccgtaaaagg aggagatttt ctttcttggt
     5101 cagaccaaag ccaattcctg gatccaacag gatattttct ggtgcaatac cagcttctgc
     5161 cgctctcgct agtgctcgtt caaagaaagc caccatcaag tcttcgattg gcaatgtttc
     5221 aaagtcagct aactcttcta ctgtaaaagt ttgattaaat ccaaaatgag ggaatatgag
     5281 cgagctaggg tgttgaggtc gcgccataac tggattaaac atgatgacca cttgcgctct
     5341 cgcttcagct accacatgag ccattttttc atcgcccatg agaccagtga tatcattaac
     5401 tagattggca ccagcagcca aagcagcctc tgctacttga ctcttccaag tatcaataga
     5461 gatgaggaca tcactttcct tgcgaatcgc tttgatcact ggaacaacac gctggatttc
     5521 ctcttctatc tcaacatagc tactgcccgg ccgagtcgat tctccgccga tatcgagcat
     5581 actggctcct tctgctatca atttacgagc ctgctggagt gcctgctcaa gagcaaaaaa
     5641 ttgaccaccg tccgaaaagg agtctggggt tacattgata attccgcaaa taactgtctt
     5701 tgcatgattg gctttacttg acatatcggt cactccctca aggattttca tcatattatt
     5761 tttctatttt accataaaaa gaaaaagatg gacacgattg cattcatctt tccctagtag
     5821 aaagaggtcg gtttctacat aaaagctttt aaacagaaat tcctaatgtc cgactcataa
     5881 tcaccacaag agccaacaaa cagaaagcaa tcccattaac aatcatgtga agcaagatcg
     5941 acatttccaa acgttgggtc ttgtaggccg tccaagatag aactgtcgac atacctccat
     6001 aaatcaataa agaaggtaaa ttacttggtt gatgcaataa agcaaacaca atcgtaccga
     6061 ctacaaatcc caagttctcc ttgcctcgga aaatcttttt aggaacaatc ccacggcaca
     6121 agatttcctc acaaatcgga gcaagcaagg ctagcaagaa gaaactggaa atcaacgaac
     6181 tattttgaac catatcgtta atctgagact ggttagctgt tgtcgtctca tttgacagtt
     6241 gcaacaagat agaaccaagt atatttgacc cgacaataac taaataactc aagcccaaac
     6301 gtgccaaatc tttagctcta aaaaaagaaa aattaaaact agctaacttg gttttacgag
     6361 ctcccataat aaatagagcc agaaccacaa ttgaaaggcc agcaactatc agccctgact
     6421 gtagcagggc tacttctttt aaagtcaaaa tagaggtaac cactagggga acctgatata
     6481 aaaggacggc aagtaaaaag accaaaatcc agcctgcacg attcaacaat tctttccaca
     6541 tatttttctc tttcatcacc tatctccttt gtattaaaaa aggggggaaa tccccctggg
     6601 tcattctatt ataaggccat gctgatgtag ttaaggataa acaaggcatc caaaatccaa
     6661 atcatggcat gaacatcttt aacttgacct ttgacaagct tagtcaaagt gtaagtcaag
     6721 aaaccaactg caatcccttg agtgatagag tagctgaatc ccataaagat agatgtgaag
     6781 aaggcaggaa ctgcttcaga catatcatcc caatggatat ttttcaagct accaagcatc
     6841 ataatcccaa cgataatcaa gattggagct gtagccgctg ttggtacgat cgctagaagt
     6901 gggctaaaga agcttgaaat cgcaaaacag atagctacaa ccaaggctgt caaaccagta
     6961 cgtccacctg caccgatacc agcagcagac tcaacataag tcgttacgtt tgaagtacct
     7021 gcaatggcac cgactgtcgt tccaatcaaa tcagagtaaa gagccttatc caatttggct
     7081 gattggtgat tttcaccatt tgtcgctaca ataccaactt tttcacctgt accgatcaag
     7141 gtaccaattg tgtcaaaaat atctgtcaat gagaaggcaa gaatggccat cagagtttca
     7201 ggcaagcgag ctgtatctga aaccaaagct cccaaacctt ctgaaccaag agctgcacca
     7261 aagattgtct tcaaatcttc aaaagctgca ccaacatggt tattagcaaa atcgatacta
     7321 gacaaatcaa ccaaaccaac tgcaatagca agaacagttg ttgtcaagat agagagaata
     7381 attccccctt taatcccttt gatgacaaag aagatagtaa tggcaagtcc tgcaagagcc
     7441 actaaaacag ctggattatt aaagctgacc aatcctggaa ctgctgaaga gtttgctgca
     7501 atcgttgctt gagctttgtc agccccttct cctacaacag tatagttgcc tggatcaatc
     7561 gtgaatttca aaagtccagc attcttaatc cctacatagg caaggaagac accgatacca
     7621 gctgaaatag ctgagcgaag agcattggga atcgattcaa tgatcatttt acgaacattt
     7681 gtcaaggtaa taatcaatga aataatccca cagatgaaga ccatagctag ggcttcttgc
     7741 caagaataac caagcccgaa tacaactgta aaggtaaaga aggcattgag tcccatacct
     7801 ggcgcttggg cataaggtaa gttagcataa aaagccatca tcaaggtacc cgctactgca
     7861 ccaataatcg tcgctaggaa gacgccctga gcaggcattc ctgtttgtga aagtatttgt
     7921 gggtttacaa agagaatata gctcattgca aagaaagttg ttaaaccagc gagaacctct
     7981 gtacgaacgt ctgtaccgtt ctcttttagt ttaaataatt tgtccatcat caatctcctt
     8041 ttaattttta cgaacaataa tagaattata tcatttatca ttcacttttt caatgtattt
     8101 gtttgatttg tttaagaaat taatccaatt tcattttttg tttggaatct gcttttctgc
     8161 ctgctttctg ttataataga agacatctta tctgaaaaca cactagaaag gtccatatga
     8221 cgaaaaaaat tattgcagtt gacctggatg gaaccctgct caactcagac agtcaaattt
     8281 ctgactttac caaaagaacc atcaaaaaag ttgctgaaaa aggccatcag gttattatta
     8341 cgacaggtcg cccttaccgt atgtcaaaag atttttaccg tgaactgggc ttagacactc
     8401 ctatgattaa cttcaacgga tcccttactc atttaccaga ccaagtttgg gattttgaaa
     8461 agtgtttgac tgtagacaaa aaatatctgc tagatatggt tcaacgttca gaggacattc
     8521 aagccgattt tatcgctgga gaatatcgta aaaaattcta cattacaaat cccaatgaag
     8581 aaattgccaa tcccaaacta tttggtgtag aagctttcca gcctgaagat caattccagc
     8641 ctgaattggt gaccaaggac cctaactgta tcctcttgca gaccagagcc agtgacaaat
     8701 atgccttggc aaaagaaatg aacgccttct accagcatca actttctatc aatacctggg
     8761 gaggtccgct caatatcctt gaatgtaccc caaaaggtgt caacaaggcc tttgctttgg
     8821 actacttgct caagataatg aatcgtgaca aaaaagattt gattgccttt ggagatgaac
     8881 acaatgatac cgaaatgctc gcttttgctg ggaagggtta tgccatgaaa aatgccaatc
     8941 cagagctact cccttatgca gatgagcaaa tttcccttac caacgaccaa gatggggttg
     9001 ccaaaaccct acaagactta ttcttataac ctatactgat actcaatgag aatcaaagag
     9061 caaactagga agctagccgt aggttgctca aagcactgct ttgaggttgt agatgaagct
     9121 gacgtggttt gaagagattt tcgaagagta tgaccagctt gcaagctggt ctttgtgctc
     9181 ttttcacagt ctcatcaacc agttaatcga ttgttctact ttttgtctcc aaaaccttgg
     9241 aaaaggcttt cttttgtgat atacttcaca tataaataca agagagctcg tcacactcga
     9301 ctctgttgac aaaaaatcaa tccagtcccg ttgtccctgt tctcggtcaa tatcaaggag
     9361 gatagctatg aaagctgttg ttgtaaatcc agaaagcact ggtgttgcta ttgaagaaaa
     9421 agtactccgt ccacttgaaa ctggggaagc acttgtagaa gttgaatact gtggtgtttg
     9481 ccacaccgac ctccacgttg ctcatggtga ctttggtcaa gtcccaggac gtgttcttgg
     9541 gcacgaaggt atcggtatcg ttaaagagat tgctccagat gtgaaaagcc ttaaagtcgg
     9601 tgaccgcgtc agcgttgctt ggttctttga aggatgtggc acttgcgaat actgtacaac
     9661 tggtcgcgaa accctttgcc gtacagtgaa aaatgctggc tactcagtag acggtggtat
     9721 ggctgaacaa tgtatcgtaa ctgctgacta tgctgtcaag gttcctgacg gacttgatcc
     9781 agcccaagct tcttctatca catgtgctgg agtaacaacc tataaagcta tcaaagaagc
     9841 aaaagttgaa ccaggccaat gggttgttct ttacggtgct ggtggacttg gtaacctcgc
     9901 tgttcaatac gctaaaaaag tattcaatgc tcatgttatc gcagtcgata tcaacaatga
     9961 caaacttgcc cttgcaaaag aagtaggcgc tgacattgtg attaacggcc tcgaagttga
    10021 agatgtagct ggactcatta aaggaaaaac tgatggagga gctcattcag ctgtcgtaac
    10081 tgctgtgtct aaagttgcct tcaaccaggc tgttgactcc gttcgtgctg gtggtcgcgt
    10141 cgtcgctgtt ggtcttcctt ctgaaatgat ggaactcagt atcgttaaaa cagtcctcga
    10201 tggaatccaa gtcatcggtt ctcttgtcgg aactcgtaaa gacttagaag aagccttcca
    10261 atttggtgca gaaggtctgg tagccccagt tgttcaaaaa cgtccagtag aagatgctgt
    10321 tgccattttc gacgaaatgg aaaaaggtca aatccaagga cgtatggtac tcgacttcac
    10381 ccactaatct aatagaaacc tattttaaaa gttggaagct tcttctaact tttttatatt
    10441 aaatttttaa ataagaattc aagattactc
//
LOCUS       DAVLXF010000070        10217 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLXF010000070 DAVLXF010000000
VERSION     DAVLXF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.070"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="VYO12_001628"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="VYO12_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1588197.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1317..2021
                     /locus_tag="VYO12_001629"
     CDS             1317..2021
                     /locus_tag="VYO12_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1588198.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKTLLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2014..3363
                     /gene="vicK"
                     /locus_tag="VYO12_001630"
     CDS             2014..3363
                     /gene="vicK"
                     /locus_tag="VYO12_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1588199.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3365..4174
                     /locus_tag="VYO12_001631"
     CDS             3365..4174
                     /locus_tag="VYO12_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1588200.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4265..4507
                     /locus_tag="VYO12_001632"
     CDS             4265..4507
                     /locus_tag="VYO12_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1588201.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4509..4763
                     /locus_tag="VYO12_001633"
     CDS             4509..4763
                     /locus_tag="VYO12_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1588202.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4810..5505
                     /locus_tag="VYO12_001634"
     CDS             4810..5505
                     /locus_tag="VYO12_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV1588203.1"
                     /translation="MKKKWLFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(5854..6840)
                     /locus_tag="VYO12_001635"
     CDS             complement(5854..6840)
                     /locus_tag="VYO12_001635"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1588204.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7034..9502
                     /gene="gyrA"
                     /locus_tag="VYO12_001636"
     CDS             7034..9502
                     /gene="gyrA"
                     /locus_tag="VYO12_001636"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1588205.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9502..>10217
                     /gene="srtA"
                     /locus_tag="VYO12_001637"
     CDS             9502..>10217
                     /gene="srtA"
                     /locus_tag="VYO12_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1588206.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTA"
BASE COUNT         3164 a         1739 c         2286 g         3028 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattc ctggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgc ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggcta ttattctcga gttcgcaata tgcaggctgc agcccagcag attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccggat cgtccgggtg
      661 attttaatca agccttgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagccgg ttcctattta tcttaaagcc ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tttggcattt cccctttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ttggccccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtacagattg tagcaggtca ggtgagtgac ttccatgatt
     1141 tttcagatag ggaagttcgt tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttctgact attttttggt ataatagtag agaaaaaggt gaacatatga
     1321 aaaaaatact aattgtagat gatgagaaac caatctcgga tattatcaag tttaatatga
     1381 ccaaggaagg ttacgaagtt gtaactgctt ttaatggtcg tgaagcgcta gagcaatttg
     1441 aagcagagca accagatatt attattctgg atttgatgct tccagaaatt gatggtttag
     1501 aagttgctaa gaccattcgt aagacaagca gtgtgcccat tcttatgctt tcagccaaag
     1561 atagtgaatt tgataaggtt atcggtttgg aacttggggc agatgactat gtaacgaaac
     1621 ccttctccaa tcgtgagttg caggcgcgtg ttaaaactct tctgcgtcgt tctcaaccta
     1681 tgccagtaga tggtcaggaa gcagatagta aacctcaacc tatccaaatt ggggatttag
     1741 aaattgttcc agacgcctac gtggctaaaa aatatggcga agaactagac ttaacccatc
     1801 gtgaatttga gcttttgtat catttagcat cgcatacagg tcaagtcatc acgcgcgaac
     1861 acttgcttga gactgtctgg ggttatgact attttggtga tgtccgtaca gttgatgtga
     1921 ctgtacgacg tctgcgtgag aagattgaag atacgcccag ccgaccagag tatatcttga
     1981 cgcgccgtgg agtagggtat tacatgagaa ataatgcttg atttactgaa acaaaccatt
     2041 tttaccagag attttatctt tatcctgatt ttgttaggtt tcatccttgt tgtgaccctc
     2101 ttattactgg aaaatagacg tgataatatt cagttgaagc aaatcaatca aaaggttaaa
     2161 gatttgattg caggagatta ttccaaggtt cttgatatgc aaggtgggtc tgaaatcacc
     2221 aatattacca ataatttgaa tgacttgtcg gaggttattc gtctcactca ggaaaatcta
     2281 gaacaagaga gtaagaggct aaatagtatt ctgttttata tgacagatgg ggttcttgcg
     2341 accaaccgtc ggggtcagat tatcatgatt aacgatacag ccaagaagca actggggttg
     2401 gttaaggaag atgttctgaa tagaagcatt ttggaattgc tcaagataga agaaaactat
     2461 gaattgcgtg atttgattac ccaaagtcca gaattgttgc tagattccca agatatcaat
     2521 ggcgaatatt tgaaccttcg agttcgcttt gccttgatac gtcgagagtc tggctttatt
     2581 tcaggtttgg tggctgtttt gcatgatacg acggagcagg agaaggaaga acgcgaacga
     2641 agactctttg tttccaatgt tagccatgag ttacggactc ctctgactag cgtaaaatcc
     2701 tatcttgaag ccttggatga gggggctttg tgtgaaactg tagcaccaga ctttatcaag
     2761 gtttctcttg atgagaccaa ccgtatgatg cgcatggtga cggatctcct ccatctttca
     2821 cgtattgata atgctaccag tcacctagat gtggaactga ttaacttcac tgcttttatt
     2881 acctttatcc tcaatcgttt tgacaagatg aaaggacagg aaaaggagaa aaaatatgag
     2941 ttggtgagag attatcccat caattctatc tggatggaaa ttgatacaga taagatgacg
     3001 caggttgtcg acaatatttt aaataatgct attaagtatt cgccagatgg gggtaaaatc
     3061 actgtcagaa tgaagacaac tgaagaccag atgattttat ccatttctga ccacggtttg
     3121 gggattccta agcaggattt accacgtatc tttgaccgtt tctatcgtgt ggatcgtgct
     3181 agaagtcgtg cgcaaggtgg tacaggtcta ggactgtcta tcgctaaaga aattatcaaa
     3241 caacataagg gctttatttg ggccaagagt gaatacggca agggttcaac ctttaccatt
     3301 gtgctccctt atgataagga tgcagtgaaa gaagaagtat gggaggatga agtagaagac
     3361 tagaatgagt gaaataggct ttaaatacag tattttagcg tcgggttcca gtggaaattc
     3421 tttttatctg gaaacctcaa aaaagaagct tttagtagat gcaggcttgt ctggcaagaa
     3481 aattaccagt ctgctagctg aaattaaccg taagccagaa gacctggatg ccatcttgat
     3541 tacccatgag cattcagatc atatccatgg agtaggcgtt ttggctcgca agtatggtat
     3601 ggatctttat gccaatgaaa agacctggca agctatggaa aatagtaaat atcttggcaa
     3661 ggtggattct tcgcaaaagc atatttttga aatgggtaaa accaaaacct ttggagatat
     3721 cgacatcgag agttttggtg taagccatga tgcagtcgca ccgcagttct atcgctttat
     3781 gaaggatgat aagagttttg tccttttgac agatacaggt tatgtcagtg accgtatggc
     3841 gggcattgtc gaaaatgcgg atggctatct tatcgaggcc aaccatgatg tagagatttt
     3901 gcgatcaggt tcttacgctt ggcgactcaa acaacgaatc ctatctgacc ttggtcacct
     3961 ttctaacgag gacggtgctg aagctatgat tcggacgcta ggaaatcgta ctaagaagat
     4021 ttaccttggg catttatcta aggaaaataa tatcaaggaa ctggctcata tgaccatggt
     4081 caatcagctg gctcaagctg atctgggagt cggagtagac tttaaggttt atgatacctc
     4141 accagatacc gcaacaccat tgacagagat atagaaagaa cgctgagaag gtgttctttt
     4201 tatattgact gaacacctaa aaagtaatac aatggtgtta ccattaaaaa agggagcaca
     4261 aaagatgact actattacat taaaagtttc tgaagctgat aaaacattta tgaaagcaat
     4321 ggctaagttt gaaggagttt ccctttcgga acttattcgc accaaaactc ttgaagccct
     4381 agaagatgaa tacgatgctc gtgtggcaga tttagcctat caagagtatt tagaagactt
     4441 ggaaaaagga gttgaaccca ttacttggga agaaatgatg catgatttag gcttgaagga
     4501 tgaataattt gtataaatta gttccaacaa gacgttttat caagcaattg aaaaaattgg
     4561 accgttatac gcagaagcta attacaaact atttacaaac caatgttttg gaaggtccaa
     4621 gacgacacgg aaaggctttg gttggtaatc gcgttggtca atggcgctat agaattggta
     4681 attatcgagt tatcgtacaa attgtagatg atgaattagt cgttgctact ctagaagttg
     4741 gtcatcggag agatatttat tgaattaaag ctgagtaata acaatataga aagtaaggag
     4801 agtaaaaatt tgaagaaaaa atggttattt gcagattatt atgatacaac aattatttta
     4861 cttgctttaa tctctgttat tctggtatta cttggatttg ctgagatgac tgatttggac
     4921 aatccgcctt atagtattat tgatttagta atctggggtg tttttgtaat tgattatagt
     4981 tggcgctttt ttattactaa aagaaaatgg cgctttattc ttgaaaatat ttttgattta
     5041 ttgaccattc tccctttaaa tgctattttt acagtgtttc gattaggacg tatcttccgc
     5101 ttagcaaggc tgacaaaatt actgaaactg actcgtttac ttagaataat tggacttaca
     5161 ggtaaactag aaagaaaaat tagtagattc ttacgaacaa atggattgat ttatatcttg
     5221 tatgttaata tctttattgt tctagtaggg agttcgattt tatccgttgt tgaagaaaaa
     5281 tccttctcag atagtctttg gtgggctctt gtgacagtaa ctactgttgg ttatggtgat
     5341 attgttccag tttccctttt tgggaaatgg ctagcagttt tactgatgct tgtcggtatt
     5401 ggaacaatag ggatgctaac aagtgctttg acgaactttt ttgtaaagga taatccagat
     5461 gaacagataa aacttgataa actcaaagac gaattatcga gctaaagaat attaatagag
     5521 aaacaatctg aaaaaatcga agaactacat tgaatgataa aagacttagt tgaaaaaata
     5581 tcataaaggt gagacaaaat aaaacttgat tcgaggagaa atcaagtaag ttttgacaca
     5641 atagaacgca tattatcagg tataaaaact tgatactatg cgttttatag agaaataaaa
     5701 taaaatctga acaaatcaat taggaattta aagcatttta taacaacgtt ttagagtaat
     5761 ggggtgctat ttcaacttca acctactata atacagaaaa aaatacagaa aaaaacaact
     5821 ccctgatgat tcaaggagtt gtctatagtt aaattagttt ttagaagctt cttggaattc
     5881 tggatttttc catgcttcgt caataatagc ttgcaattct ttagcagatg cttgcatttt
     5941 ttgagtttct gcatcgttca atggaatgtt tactggacga acgataccat gtgcaccaac
     6001 aacagctggt tgaccgataa agacattctc aactccgtat tgaccttctt ggaatactga
     6061 aagtggaagt actgcgtttt catcgtcaag gattgcttta gtgatacgag caagggctac
     6121 tgcgataccg tagtatgttg cacctttttt gttgatgatt gtgtaggctg catcacgaac
     6181 accttcgaac aattcaatca attcagcttc ttgaacattt tgagtgtctt taaggaattc
     6241 ttcaagattt acaccagcga tgttagcgtg tgaccaaaca gcgaactcag agtcaccgtg
     6301 ttcacccatg atgtaggcgt gcactgaacg agcatccaca tccaattttt cagcaagtgc
     6361 ttgacggaaa cgagctgagt caagtgaagt acctgaaccg ataacgcgtt ctttagggaa
     6421 accagagaat ttccaagttg agtaagtcaa aacgtcaact gggttagcag caacaaggaa
     6481 gatacctttg aaaccagatt caacaacttg agttacgatt gatttgttga tagcaaggtt
     6541 tttacctaca aggtcaagac gagtttcacc tggtttttga ggtgcacctg cagtgatcac
     6601 aacaaggtca gcgtctgcac agtcagagta ttgagctgca tagatttttt taggtgaagt
     6661 gaaggcaagg gcgtgactaa ggtcaagcgc atcaccaaca gctttttcat gcaattgtgg
     6721 aatttcgata attccaagct cttgtgcaat tccttggtta acaagtgcaa aagcgtaaga
     6781 tgaacctaca gcaccatcac cgacaaggat aacttttttg tgttgtttag ttgaagtcat
     6841 tgttttaaac atctccttaa ttttattagg ggattttccc tagacaactt cattctatca
     6901 cttttaaaaa actttgtcac gaatatgcct tatagttctc gatgtaaacg ttttagtggt
     6961 ttagaggctg aaatagatgg gaatttatgg tataatgttg ttacttacta attgtgaaat
     7021 gaggcattta ttaatgcagg ataaaaattt agtgaatgtc aatctgacaa aggagatgaa
     7081 ggcaagtttt atcgactacg ccatgagtgt tatcgtagcg cgagctcttc ctgatgttcg
     7141 agatggctta aaacctgttc accgtcgcat tctctacgga atgaatgaat tgggtgtgac
     7201 cccagacaaa ccccataaaa aatctgctcg tattacaggg gatgtcatgg gtaaatatca
     7261 cccacacggg gattcctcta tttatgaagc catggtccgt atggctcaat ggtggagcta
     7321 ccgttacatg cttgtagatg gtcatgggaa ttttggttcc atggatggag atagtgctgc
     7381 cgctcaacgt tataccgagg cacgtatgag caagattgct ctggaaatgc ttcgtgatat
     7441 caacaaaaat acagttgatt tcgttgataa ctatgatgcc aatgaacggg aacccttggt
     7501 cttgccagcg cgttttccaa accttttggt taatggagca actggtatcg cggttgggat
     7561 ggcaaccaat attccacctc ataatctggg tgaaaccatt gatgcagtga agttggtcat
     7621 ggataatcct gaagtgacta ccaaggactt gatggaagtc ttgcctggac cagattttcc
     7681 aactggtgct cttgtcatgg ggaaatcagg tatccataag gcttatgaaa caggtaaagg
     7741 ttcgattgtc ctacgttctc gtacagagat tgaaacgact aagactggtc gtgagcgtat
     7801 cgttgtaaca gaatttcctt acatggtcaa taaaaccaag gtgcatgagc atattgttcg
     7861 cttggttcag gaaaaacgca ttgagggtat cacagcagta cgtgatgagt caaaccgtga
     7921 aggtgttcga tttgttattg aagtcaagcg cgacgcctca gccaatgtta ttctcaataa
     7981 cctcttcaaa atgacccaaa tgcaaaccaa ttttggattc aatatgctcg ctatccaaaa
     8041 tggtataccg aaaattttgt ctcttcgtca gattttggat gcttatattg agcaccaaaa
     8101 agaagtggtt gttcgtcgta cacgttttga taaggaaaaa gcggaagcgc gcgctcatat
     8161 cttagaaggt ctcttgattg cgctagacca tatcgacgaa gtgattcgta tcatccgtgc
     8221 tagtgaaacg gatgcggaag ctcaagctga gttgatgagc aagtttaagc tttctgaacg
     8281 tcaaagtcaa gctatccttg atatgcgtct tcgtcgtttg acaggtttgg aacgcgataa
     8341 gattcaatct gagtatgatg acctcttggc tctgattgcg gatttagcag atattcttgc
     8401 taagcctgaa cgtgtttctc aaattatcaa agacgaattg gatgaagtta aacgtaaatt
     8461 ttctgataaa cgccgtacag agttgatggt tggacagatc ttgagtctcg aggatgagga
     8521 cttgattgaa gaatcggatg tcttgattac cctttctaac agaggctaca ttaagcgttt
     8581 ggatcaggac gagttcactg ctcaaaaacg tgggggtcgt ggtgtccaag gaacgggagt
     8641 gaaagatgat gactttgttc gtgagttagt gtcaactagc acccatgatc atctgctctt
     8701 cttcacaaac aagggacgtg tctatcgtct taaaggttat gaaattcctg agtatggtcg
     8761 gactgccaaa gggctaccag tagtcaatct cttgaaattg gatgaagatg aaagtattca
     8821 gacggttatc aatgttgagt ctgatcgcag tgatgatgct tatctcttct ttacaacccg
     8881 tcacggtatt gtgaagagaa ctagtgttaa ggagtttgcc aatattcgtc aaaatggtct
     8941 caaagcgctg aatttaaagg atgaagatga gttaatcaat gtcttgttga cagaaggaga
     9001 tatggatatt atcattggta ccaagtttgg ttatgcagtt cgctttaatc aatcagccgt
     9061 tcgtggtatg agccgtatcg ccactggtgt gaaaggtgtt aaccttcgtg aaggagacac
     9121 agttgttggt gccagcttga ttactgatca agatgaggtt cttattatca cagaaaaagg
     9181 atatggtaag cgtacagtcg ctactgaata cccaacaaaa ggtcgtggtg gtaagggaat
     9241 gcagacagct aaaattaccg aaaaaaatgg cttgctggcc ggtcttatga ctgttcaagg
     9301 ggatgaggat ttgatgatta tcactgatac aggtgtcatg attcgaacca atcttgccaa
     9361 tatttcacaa acaggacgtg caactatggg agttaaagta atgcgcctgg atcaagatgc
     9421 tcagatagtg actttcacaa cggttgcggt ggcagaaaaa gaagaagttg ggacagaaaa
     9481 cgaaacagaa ggtgaagcat aatgtctcaa aaaaataata aaaagaaaaa caagcgaaaa
     9541 aatctgctga caaatatcct agcaggattt ctgatattac tgtcactggc tttgattttt
     9601 aatactcaaa ttcgaaatat tttcatagtc tggaatacca ataagtatca agttagccag
     9661 gtatcaaaag aaaaattaga agaaaatcag gatacagaag gcaattttga ctttgattct
     9721 gtcaaagcta tctcttcgga agctgttcta acttctcaat gggatgctca aaaattacca
     9781 gttattgggg gaattgcaat tcctgaattg gaaatgaatt tgccgatttt taaaggactt
     9841 gataatgtta atctcttcta cggagctggt acaatgaaac gcgagcaagt aatgggagaa
     9901 ggaaattata gtctagctag tcaccatatc tttggtgttg ataatgctaa taaaatgtta
     9961 ttttctcctt tagataatgc taaaaatggc atgaagattt atctaaccga taaaaataaa
    10021 gtttatactt atgaaatacg tgaagtcaaa cgtgtgacac cggatcgtgt tgatgaagtt
    10081 gatgatagag atggggtcaa tgaaatcaca ttagtaacct gtgaagacct tgctgctaca
    10141 gaacgtatta ttgtcaaagg tgatttgaaa gaaacaaaag attattcaca aacatctgat
    10201 gaaattctaa cagcttt
//
LOCUS       DAVLXF010000071         9867 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLXF010000071 DAVLXF010000000
VERSION     DAVLXF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.071"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            186..1022
                     /locus_tag="VYO12_001638"
     CDS             186..1022
                     /locus_tag="VYO12_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1588207.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIIIGKGIAFGKRKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLKGYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            1058..1375
                     /locus_tag="VYO12_001639"
     CDS             1058..1375
                     /locus_tag="VYO12_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1588208.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            1375..3066
                     /locus_tag="VYO12_001640"
     CDS             1375..3066
                     /locus_tag="VYO12_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1588209.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            3275..4681
                     /gene="lacG"
                     /locus_tag="VYO12_001641"
     CDS             3275..4681
                     /gene="lacG"
                     /locus_tag="VYO12_001641"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1588210.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTDYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            4724..4981
                     /locus_tag="VYO12_001642"
     CDS             4724..4981
                     /locus_tag="VYO12_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1588211.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDELEEFKNLFLNFINPEGWDTISFDSDFMPFQQS"
     gene            complement(5260..6021)
                     /locus_tag="VYO12_001643"
     CDS             complement(5260..6021)
                     /locus_tag="VYO12_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1588212.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(6203..7165)
                     /gene="nrdF"
                     /locus_tag="VYO12_001644"
     CDS             complement(6203..7165)
                     /gene="nrdF"
                     /locus_tag="VYO12_001644"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1588213.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(7353..9512)
                     /gene="nrdE"
                     /locus_tag="VYO12_001645"
     CDS             complement(7353..9512)
                     /gene="nrdE"
                     /locus_tag="VYO12_001645"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1588214.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(9593..9811)
                     /gene="nrdH"
                     /locus_tag="VYO12_001646"
     CDS             complement(9593..9811)
                     /gene="nrdH"
                     /locus_tag="VYO12_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1588215.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         2971 a         1792 c         2001 g         3103 t
ORIGIN      
        1 gttgtatgta aaggtttaca aaacaaccga cttgtgctat acttaaatca caagttaata
       61 caaggtgagt gttactaagt aatattaggc atgatcacag gtgaattaga aatcagctga
      121 ttttctagtt catttgtggt cattttttgt actcatatac ctttaagata taaaaggagg
      181 ttgacatgta tcgaattcta aatccaatga atcacaatgt ctcgcttgtc agaaatgata
      241 agggagaaga ggtgattata attggtaagg ggattgcatt cggaaaaagg aagggggatt
      301 tgattgctga aaatcaggtt gagaaaatct ttcggatgaa gaccgaagag tccagagaaa
      361 actttatggc tcttctcaaa gatgttccgc ttgattttat cacagtgacc tatgaaatca
      421 ttgataagct atcaaagaaa tatcattatc cgattcaaga gtatctctat gtaaccttga
      481 cagatcatat ttactgttct tatcaagctc taactcaagg aaggtacaag gatagtaatc
      541 tgccggatat ttccgctaag tatcctgtcg cttttcaaat cgcaaatgaa gcttttgaaa
      601 tttaccgtca gaagctagca gatcattttc ctgaggacga gattattcgg attgcttatc
      661 atttcatcaa tgccgaaggt gaaaatgaag tggaacttgt ggagtcgatt gataagagga
      721 aagaaattct caggaatgtt gaagaagtgc taaagggtta tgcaattcaa cgaaccaaaa
      781 aaaataatca tttctatgat cgctttatga ttcatttgaa ttatttcttg gattatttag
      841 acagatctag agatgataac caatcacttc tggatatgga agatcatatt aaacaatcct
      901 atccaaaagc cttcgagatt ggttccaaga tctatgatgt gattacgcaa catacgggtc
      961 ttgatttgta taaaagtgaa cgagtttatc tagttctaca tatccaacgt ttattgtcat
     1021 aaaaatttat ttaaaactat ataaggagaa ttctatcatg aatagagaag aagtaacatt
     1081 gttaggtttt gaaatcgtag cctatgctgg cgatgctcgt tcaaaactat tggaagcctt
     1141 gaaggctgct gaagctggtg attttgaaaa agcggacgct ctggtagagg aagctggtag
     1201 ctgtattgca gaggctcacc acgcgcaaac aagtctattg actaaggaag cttctggtga
     1261 ggacttagcg tatagtgtga ccatgatgca tggccaagac cacttgatga caactatctt
     1321 gttaaaggat ttgatgcacc atttgatcga actctataag agaggagtta aataatgaac
     1381 aaactaattg catttatcga gaaaggaaag cctttctttg agaaactatc tcgtaatatc
     1441 tatcttcgtg ctattcgtga tggtttcatt gcaggtatgc ctgttattct cttctcaagt
     1501 atctttatct tgattgcctt tgtaccaaac tcatggggct ttaaatggtc tgatgaagtt
     1561 gtagcctttc tgatgaaacc ttatagctat tctatgggta ttctggctct cttggtagct
     1621 ggtacaacag ctaagtcatt gactgactca gtaaaccgga gcatggaaaa aaccaatcaa
     1681 atcaactata tgtcaacgct gttagcagcg attgttggtt tgttgatgtt ggcagctgat
     1741 cctatcgaaa gtggtctagc tactggattc ttggggacaa aaggtttgct ttcagccttc
     1801 cttgctgcct tcgttactgt agccatctat aaggtttgtg ttaagaacaa cgtcactatt
     1861 cgtatgcctg acgaagttcc accaaatatc tcacaagtct ttaaagatgt gattccattc
     1921 actctatcag tggtttctct ttatgctctt gatttactag ctcgtcattt tgttggttct
     1981 agtgtggcag aatcaatcgg taaattcttc gcaccactct tctcagcagc agacggatac
     2041 cttggtatta ccattatctt tggtgccttt gccttcttct ggtttgtggg tattcatggt
     2101 ccatctattg ttgaacctgc tatcgcagcg attacctatg caaatgctga agtcaacttg
     2161 aaccttctcc aacaaggtat gcatgctgat aaaatcctta cttcaggaac tcaaatgttt
     2221 atcgttacta tgggaggtac tggtgcgaca ctggttgttc cattcatgtt catgtggtta
     2281 acaaaatcaa aacgtaaccg tgcaatcgga cgtgcttcag tagttccaac tttctttggt
     2341 gtaaatgaac caatcttgtt tggtgcacca cttgtattaa atccaatctt cttcattcca
     2401 tttatctttg ctccaatcgc aaacgtatgg attttcaaat tctttattga aactcttgga
     2461 atgaactcat tcactgctaa tctaccatgg acaactccag ctccactagg tctagttctt
     2521 ggaactaact tccaagtgct atcattcatt cttgctgccc ttctaatcgt ggttgacgtt
     2581 gtcatttact atccattcct taaggtctat gatgaacaaa ttcttgaaga agaacgttca
     2641 ggtaagtcta atgatgaatt gaaagaaaaa gttgctgcaa acttcaacac tgcaaaagcg
     2701 gatgctattc ttgaaaaagc gggtgtagaa gcagcacaaa atacaatcac taaagaaaca
     2761 aatgtcctcg ttctctgtgc aggtggagga acaagtggtc tccttgcaaa tgctttgaat
     2821 aaggcagcag cagaatacaa tgtccctgtg aaagcagcag caggcggcta tggtgctcac
     2881 cgtgaaatgt taccagagtt tgatcttgtt atccttgccc ctcaagttgc ttcaaacttt
     2941 gaagatatga aggcagaaac agataagctc ggtattaaac tagcgaaaac agaaggcgct
     3001 caatacatca aattaactcg tgatggaaaa ggtgctcttg cattcgtaca agcgcaattc
     3061 gattaaggct agagactctg aaatagtctc ctatcgttac ggaaatcgct atggcgaatt
     3121 tcctattatt aattcgtcgg taaaaagata tcgtttttac ctcctcatgt cacaattcgg
     3181 tgacttggta caagaagtga gatggagaag gatggctcac tgactcctct cctctcactt
     3241 ttactttatt taaatcaaga aataggtgaa aaaaatgaca aaaacacttc caaaagactt
     3301 tatttttggt ggcgcaacag ctgcctatca agcagaaggt gctacacata ctgatggtaa
     3361 aggaccagta gcgtgggata aatatcttga agataactac tggtacactg cagaaccagc
     3421 cagtgatttt tacaatcgat atccagttga cctcaagcta gcagaagagt atggtgtcaa
     3481 tggtattcga atttctattg cttggtcacg tattttcccg actgattacg gccaagtaaa
     3541 tgctaaaggt gttgagtttt atcataattt atttgcagag tgtcacaaac gtcatgttga
     3601 gccttttgta actcttcatc actttgacac gccagaagct ctccactcaa atggagactt
     3661 cttaaaccgt gaaaatatcg aacattttgt agactacgct gccttctgtt ttgaagaatt
     3721 tccagaagta aactattgga caacctttaa tgaaattgga ccaatcggtg atggtcaata
     3781 tttggttggg aaattccctc caggtatcca gtacgacctt gccaaagtct ttcaatcaca
     3841 ccacaatatg atggtgtctc atgcacgcgc ggtaaaattg tacaaagaga aaggctataa
     3901 aggggaaatt ggtgttgttc acgccctgcc aactaaatat cctctagatc ctgaaaatcc
     3961 agcagatgtt cgtgcagctg agttggaaga tatcatccac aataaattca tcttagacgc
     4021 aacttatcta ggtcgctatt cagctgaaac catggaaggt gtcaaccata tcttatcagt
     4081 caatggtggt agcttggatc ttcgtgaaga agattttaca gcattagaag ctgcaaaaga
     4141 cttgaatgat ttcctaggaa tcaactacta tatgagtgac tggatggaag cctttgatgg
     4201 agaaactgaa attatccata atggtaaagg tgaaaaagga agctctaagt atcaaatcaa
     4261 aggtgttggt cgtcgtgtag ctcctgacta tgtaccacgc acggattggg attggattat
     4321 ctaccctcaa ggtttgtatg accaaatcat gcgtgtgaag aaagattatc ctaactacaa
     4381 gaagatttac atcactgaaa atggtctcgg ctataaagat gagttcgttg ataacactgt
     4441 ttacgatgat ggtcgtattg attacgtgaa acaacacttg gagatcttgt ctgatgcgat
     4501 tgcagatgga gctaatgtaa aaggttactt catttggtca ttaatggatg tcttctcatg
     4561 gtcaaacggt tatgagaaac gttatggtct cttctacgta gattttgaaa ctcaagaacg
     4621 ttatcctaag aaatcagctc actggtacaa gaaagtagcg gaaactcaga ttatagacta
     4681 gtagaattag tcattagata tagaatttta gtgagtcaaa aagatgttca aagattttat
     4741 ccaatctatt tatgaaaaag tttatattat aaatttcgaa aaatgctctc aaataccgtg
     4801 tttgacgagt gaagaattga aaagtcttgg aaaatggtat gtctcgactg gtaaagaatg
     4861 gatttgtcat tcagatgatg agctggaaga atttaaaaat ctatttttaa attttatcaa
     4921 tcctgaagga tgggatacta tctcctttga ttcagatttt atgccgtttc aacaatcgta
     4981 accaatttct caaaaaagtt aaatcttata tttagtactc tgtaaaactc ttatctaatc
     5041 acgttgctta tactcaatga aaatcaaaga gcaactttaa actaggaagc gagtcgcaga
     5101 tttctcaatg catagctttg aggaattggg caaaaagtct ttgatataga aaaacgcata
     5161 gtatcaggtg ttgaaacacc tgatactatg cgttttattg tgggaagatt tacttttttt
     5221 cttctgaaat tgagttgtta cccaggctct ttcagtttat taaggcttga tgactttaat
     5281 gtgtttagat agcttaaaaa ggattgaatc acttagttta gaatctgaaa caatagtatc
     5341 aagatttgat acattataaa aagtataaaa atcaaactta ttgaacttgc tatgatctgc
     5401 gagtaaatat tttttattag aattatttaa agcgatgcgt tgagcctctc cctcttcctc
     5461 gctaaaagta gctagagctc cgttttgaat accattacag ctaacgaaag ctttagaaaa
     5521 ttggagatta gagagatttt gtagggtcaa tgtaccaaca aaagcacctg taatatcgcg
     5581 ataatttcca cctattaaaa tcaaatctgt taattttcgt tcgcttaaaa tcagaaaaac
     5641 aggtagactg ttggttacga cgcggatatt gtcaataggc aactcacgcg caaaaaactc
     5701 taatgttgtt cctggtccaa tgaaaatagt ttctctttct tctactagac tgcctgcaaa
     5761 atgggctatt tcttgttttt ctgccgtttg gagggcttgt ttttcaatat ttgatcgctc
     5821 attagtcaaa agggagttgg ttcgaagttt ttcagctcca ccatgcacac gaatcagcaa
     5881 atctttatca gctaattcct gtaaatagcg ccttgcagtc atatctgaaa cggctatttc
     5941 gtccataatc tgtttaactg ttatggttcc tttactattt actatctcta aaattttggc
     6001 taatttttct tgtttgagca tattatcacc tcgtttccta ctactatctt accataaaca
     6061 aactcatcat tcaaatacaa aaacaacaaa atgaaacaaa aacaaaaata tcgaagtttg
     6121 ttttcaaaac tttcgatatt tttgttgggt tataactttg atgtttctag tttacttttt
     6181 gatgattgag agtgatggag aattagtcta aaccgtagtt atagtcatcg tcttgcatgg
     6241 cttcaacttc gccaagaagg taaccatttc cgacttgaga gaagaagtca tggttggaag
     6301 ttcctgttga aataccgttc ataacgattg ggttgacatc ttcagctgaa tctgggaaaa
     6361 gtggatcttg tcccatgttc atgagagctt tattggcatt gtagcgaagg aaggttttaa
     6421 cctcttcagt ccaaccaaca ccgtcataaa gactctctgt gtagccttct tcattttcat
     6481 aaagagtata gagtaggtcg tacatccatt ctttgagttt ttcttgctct tcttcaggca
     6541 attcattgaa accaagttgg aatttgtaac caatgtaggt tccgtgaaca gactcgtcac
     6601 gaataatcaa tttaatgatt tctgcaacgt tggcaagttt gttgttaccg agatagtaga
     6661 ggggagtgaa gaaaccagag tagaagagga aggtttcgag gaagacgctg gcaactttct
     6721 tttcaagtgg gctgccgttt aggtagattt cgttgacaat ctcagccttc ttttgtaggt
     6781 aaggattggt attggtccat tcgaaaattt cttcaatctc agccttagta ttcaaggtag
     6841 aaaagattga tgagtaagat ttagcgtgga cagattccat aaattggatg ttattgaaaa
     6901 cagcttcctc atgtggtgta cggatgtctg cgcgaagggc ttgaacccca gtttcagatt
     6961 gcatagtgtc aagaagggtt aaaccaccaa aaacttttcc gaccaagtct ttctctttgt
     7021 tagatagctt tctccagtca tccaagtcgt ttgataaggg aatacgtgta tcgagccaaa
     7081 attgctccgt cagtttttcc caagttgatt tgtcgatgac atcttcgatg gcattccagt
     7141 taatggcttt gtagtaagtt tccatttaaa atctctttct gtgtttagta ttgcgaactc
     7201 acaattattt ctactttacc ataattctat aggagtatcg cacaaaaagt cggaagcccg
     7261 acttttaaaa tgttacataa attatgttat gacatagtag atttgatttt atcagtgctg
     7321 cttagggaaa aatagtgttt ctatgctaga aactaaatca cacagctttc acattggttg
     7381 gcgccgactt ctccaccgtc atctgtaaag gtacggacgt agtagataga cttgattccc
     7441 ttgttaaagg catagttacg aaggatggac aaatcacgtg tcgtttgttt attttccctc
     7501 ttccattcgt aaaggccttt tggaatgtca ctacgcatga agagggtgag tgaaagtcct
     7561 tgatccacgt gttcagtcgc agcagcgtaa acatcgatga ctttacgcat atccatatcg
     7621 taggcagaag tgtagtaagg aatggtttct gtagacaagc cagcagcagg gtagtagatt
     7681 ttaccaattt tcttctcttg gcgttcttcg atacgttgcg taatcgggtg gatagaagca
     7741 gaaacgtcgt tgatatagct gatagaacca tttggcgcta cagcaaggcg attttggtgg
     7801 taaagaccat cttcttgaac cttgtcgcga agttcagccc aatcagcaac accagggata
     7861 aagacatttt tgaagagttc tttaacacgg tctgatgttg gaacaaattc accagttaca
     7921 tacttgtcaa agtaacttcc gttagcatag tctgattttt caaagttgtg gaaggtaata
     7981 ccacgttcac gcgcaatatt gtttgactct accaaggtcc agtagttcat aagcataaag
     8041 tagatgcttg taaattcaac agactcaggt gaaccatatt caatgagttg ttgggcaagg
     8101 tagctgtgca gtcccatggc accgagacca aaggtgtggg cttggctatt tccatggtca
     8161 atcgttggta cagctacgat atgtgaacta tctgtaacga aagtaagggc acgaaccata
     8221 gcacggatag aacgaccaaa atcaggtgaa gtcatcatgt taaccacgtt ggttgaaccc
     8281 aggttacatg aaacatctgt tcccatttga aggaattctt gagcatcgtt gatcaagctt
     8341 ggttcttgaa cttgaagaat ctcagaacac aagttactca tgataatctt tccatcaaca
     8401 ggatttgcac ggttagccgt atcgatgttg actacataag gatagccaga ctcttgttgc
     8461 aatttagaga tttcagtttc caaatcccgc gccttgattt ttgtcttgcg aatatttgga
     8521 tttgcgacca attcatcgta tttttcagta atgtcgatgt aattgaatgg cacaccgtat
     8581 tctttttcta cagagtaagg gctgaagagg tacatttctt catttttacg agccaattcg
     8641 tagaatttat caggtactac aacaccaagt gatagagtct tgacacgtac tttttcatca
     8701 gcgttttctt tcttagttga aaggaaagcg atgatatctg ggtgaaagac gttgaggtag
     8761 acaacaccgg caccttgacg ttgccccaat tggttggagt aagagaagct gtcttcaaaa
     8821 agcttcataa caggaacgac acctgaagca gctccttcat agcctttgat aggtgcacca
     8881 gcttcacgaa ggttgctgag ggtaattccc acaccaccac caatacgtga aagttgaaga
     8941 gctgagttga tagaacgtcc gatagagttc atatcatccg tcacttggat taggaaacaa
     9001 gataccaact ccccacggcg agcacgtcca gcattcaaga aggaaggagt agcaggttgg
     9061 tagcgttggt ggatgatttc attggcaata tcgattgcaa cagcttcatt cccatcagcg
     9121 aaataaaggg cgttgaagaa gacacggtct tccatatttt caagatagta ttcaccgtca
     9181 ttagtcttta aggcatattg attgtaaaat ttataagctg ccatgaatga cttgaattgg
     9241 aagttttggt ctttgataaa ttgagccaat tcttccaaga actctggacg gtatttcttg
     9301 ataaaggctg tttcgatgta gttgtgttca atgaggtaat tgattttgtc tttgattgaa
     9361 tcaaaaacca tagtgtttgg aactacattt tctttaaaga aagcatccaa ggcttccttg
     9421 tctttatgaa gcatgatttg tccattaaca ggacggttaa tttcgttatt aagacggaag
     9481 taagtcacgt cttcaagatg ttttaatccc ataaaatttc ccttatctaa ttacaaaaga
     9541 aaggcttcta agttagccct aaaagcagtt ttttctggat gatgtactaa gattatgcta
     9601 attgcttcag ttttcctggt tggaaacctg aaaagacttc agttggtgtt tggataacag
     9661 gagctgcgct aaaaccgagc tctttaactt gatcgacgta ctcaggttgc tcatcaagat
     9721 tgatttcacg ataagagaca ttattactgt ccaagaaacg cttggtcatt ttacattgga
     9781 cacaattgtt tttagaataa acggttacca ttgtgtaact cctcttcaaa atttaatact
     9841 atcttagtat atcagaaaat aaaattt
//
LOCUS       DAVLXF010000072         9852 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLXF010000072 DAVLXF010000000
VERSION     DAVLXF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.072"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(35..271)
                     /locus_tag="VYO12_001647"
     CDS             complement(35..271)
                     /locus_tag="VYO12_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1588216.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(339..1259)
                     /locus_tag="VYO12_001648"
     CDS             complement(339..1259)
                     /locus_tag="VYO12_001648"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1588217.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDSDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            1449..1852
                     /locus_tag="VYO12_001649"
                     /pseudo
     CDS             1449..1852
                     /locus_tag="VYO12_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2068..2649
                     /locus_tag="VYO12_001650"
     CDS             2068..2649
                     /locus_tag="VYO12_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1588218.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            2646..3923
                     /locus_tag="VYO12_001651"
     CDS             2646..3923
                     /locus_tag="VYO12_001651"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1588219.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            3941..4603
                     /gene="deoC"
                     /locus_tag="VYO12_001652"
     CDS             3941..4603
                     /gene="deoC"
                     /locus_tag="VYO12_001652"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1588220.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            4590..4979
                     /locus_tag="VYO12_001653"
     CDS             4590..4979
                     /locus_tag="VYO12_001653"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1588221.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            5069..6121
                     /locus_tag="VYO12_001654"
     CDS             5069..6121
                     /locus_tag="VYO12_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1588222.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            6265..7800
                     /locus_tag="VYO12_001655"
     CDS             6265..7800
                     /locus_tag="VYO12_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588223.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINMHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGAINADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            7793..8851
                     /locus_tag="VYO12_001656"
     CDS             7793..8851
                     /locus_tag="VYO12_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1588224.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLTELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            8854..9810
                     /locus_tag="VYO12_001657"
     CDS             8854..9810
                     /locus_tag="VYO12_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1588225.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         2800 a         1756 c         2278 g         3018 t
ORIGIN      
        1 ttcttcaacc cactacagtt gacaaagagc cttcttattt agcaagttta gctgaaagac
       61 gagctttatc gcggcttgct ttgtttttgt gaatcaaacc tttagtttct gctttatcga
      121 tagctgagct agcagcacgg aaaagttctt cagatgggtt tgcttcgaaa gcttttatag
      181 cagtacgcat agctgatttt tgagctgagt tcttttcgtt ttgtttaacg ttcaattcag
      241 cgcgtttgat agctgattta atgtttgcca atgttcttac ctccatattt actaactata
      301 ccattatata tgaaaactta cgttttgaca agggaaaatt atttttttaa gtaaatttca
      361 tcgatttcat ggttcttgct tttatgaaga atcacttctg cacgatttct ggttggttca
      421 atataatttt gtagatttgt gagattgata ctggtccaga cctgatgggc aaaggcttcc
      481 acttccccaa tcggcatctg agtaaaacga taatagtagc tatcagagtc gttttgggct
      541 agactcagca ttttcaagaa acggtccaga taccaacttt caatatcatc cactccagca
      601 tctacataga tggaaaagtc aaagaagtca gtgatataga gacgatcgtt ttgtggattt
      661 tgaaagacat tgattccctc aacaattaca aaatcagcag ctttgacact ttgtttctct
      721 tcgggtacga tgtcgtaaac ttcatgagaa tagacaggaa tatctacatc ttgtccattt
      781 ttgatgcggt ccaagaagtt gagaagagct tccatatcat agctttcagg aaatccttta
      841 cgatttaaaa tcccctgctc aatcaaggtt tgattgggat agagaaaacc atcagttgta
      901 accaactcaa ccgtagcatc tgtaaacgta cgggacagta ggatttgaag taggcgactg
      961 gttgtggatt ttccaacggc aacactccca gaaaccccaa taataaaagg ttgagattta
     1021 ctttcacgtt ggaggaaaat tccttttgaa aaggctaaat cttccttagt tcgcttgtaa
     1081 atctgaatca gatgagccaa ggggagatag atatctgtaa cgtcttggag actgatttgg
     1141 tcattaaaac tcttgataga ttccaattct tcttctgtca aaggaggtgt tgtctttcga
     1201 tgtaaagatt gccaagtctg gcggctgatt ttttcaaaat gtaaaaattc gttggtcatt
     1261 tgttttcccc tagatatttt gtctatcata ccataaaaag ctaaaaaaat aaagcggttt
     1321 cttgaatgtg gtaagtcaat ggtatatagt ataagtgatc aatttattga tgaaatagga
     1381 gatagattct actgatttat aagcttataa agggggcttt ttagttcaca acttatagga
     1441 ggtgtactat gaaaatctta aaacgttaca tattggaact ctgttttatt ttaagttttg
     1501 ctttaccttt tataaaagga gccaatgcag ataatggtag atgctttgtg gaaacctatt
     1561 acggttttac ttttttgatg gaacatgcta ttgtaacagc tgtctttatc tgttcgttct
     1621 taattgcttt cttactaaaa aacgatgggc gaaatggatt gctgcgggta gttattgctt
     1681 tttagtccta cggattgcga cagagggtta tttcttccac atgtcactag aagatttgat
     1741 acgactttgg acaagtttgg aaatcctgac acaaacgtat cagttgggct tttatctaaa
     1801 cattttgtta ggaattttgt tgataataaa gtattttaag gttaagcaat agaaataata
     1861 atctgcctga ttatagatga taaacttttt gtttttatct ggactgtgat atttatagtt
     1921 ctaaatggtt tattatttat aagtgttgaa agagattgaa gaaaatggct tcgtgacttg
     1981 ctttcttcgg tctttttcgt tttattatca atttgtaaac gatttacaat ttgagtcaaa
     2041 ttatggtaaa atagtgtcat gagtaaaatg tactatgcag aaaatcctga cgctgctcac
     2101 gacattcatg agttgagagt ggacttgttg ggagaaaaaa tgactttttt gacagatgcg
     2161 ggtgttttta gcaagaaaat ggttgacttt ggaagtcaac tcttgctcaa gtgtctggat
     2221 gtcaaccaag gagagacagt cctagatgta ggctgtggct atggtccatt aggtttgtcc
     2281 ttggtcaagg tttacggagt tcaggcgacc atggttgata ttaacactcg tgccctggat
     2341 ttagctcaga gaaatgctga aaaaaataat gcaaaagcga cgatatttca atctaatatt
     2401 tatgcaagag ttcaagggcg atttgaccat gtcatttcca atcctcctat ccgtgcgggc
     2461 aagcaagtcg ttcatgaaat catcgagaaa agtaaagatt tcttggaaat tggtggagat
     2521 ttgaccatcg tcatccagaa aaaacaagga gctccaagtg ccaagtctaa gatggaagat
     2581 gtgtttggca attgtgaaat ccttaaaaaa gacaagggat actatatcct tagaagtgtg
     2641 aaagaatgag agcagttgat ttaatccaaa aaaaacgaga cggtcaagaa ctgacttcaa
     2701 gtgaaattga atggctgata gaaggctatg tatcaggaac tgttcctgat tatcagatgt
     2761 ctgcctttgc tatggctgtt tattttaaag gaatgacgac tcgagaaatc tctgatttaa
     2821 cgatgaatat ggttaagact gggcaagagt ttgacctgtc agccattgat ggtgtgaaag
     2881 ttgacaagca ttctactggt ggtgtcggcg ataaggtaac cttgatttta gctcctcttg
     2941 ttgctagctt cggtgtgcct gtagcaaaaa tgagtggtcg tggtcttggc catacaggcg
     3001 gaacaattga taaattggag tccattaagg gctatcaagt agaacgtagt caagaggatt
     3061 ttattcgtca ggttcaggat attggagtat ctgtcattgg gcaatcagac cagctggtca
     3121 aagcagataa gcttctctat gcccttcgtg atgtgaccgc aactgtcgac acgattcctt
     3181 tgattgcgag ttcggtgatg agcaagaaaa ttgcggcagg ggcggatgct attttgctag
     3241 acgtgactgt cggtgagggt gccttcatga agacggttga tgaggcgcgt gagttggctc
     3301 aaactatggt agatcttggt aaggttgttg gacggaagac agtagcagtg attacggata
     3361 tgagccagcc tttgggacga gcgattggaa atcgtctgga aatccttgaa gcattggaga
     3421 ttttgcaagg tcaaggacgt caggatatta cccactttat ttgtgaattg gctcaaatta
     3481 tgcttggttt ggcaaatgtt aacaaaactg ttgaagaagt tcgccaacat cttgaaaatg
     3541 gtcaagcact ggctaagttt gaggaaatgg tgcaagccca aggtggagac ttggaagacc
     3601 tctatcgtcc tgtaaatgta gctcatgtgg tggaaatccc tgctcaggaa acgggtgtca
     3661 tttcagctct tccagctatg gactttggtc tttatgccat gaggctgggt gctggtcgtg
     3721 cagttaagtc tgatgtcttg gactatgaaa caggaattgt ttttgaaaag aagattggag
     3781 actccgttca aaagggtgaa attgttgcaa aagtatacac aaatgaaaaa attccacctc
     3841 aactagttac agatttccaa aaatgtgtta aaataagtga tgaagtgaaa aaaatccgtg
     3901 aaattgtaga aatcatctcc taaatggagg gaaaaccgaa atgaaattaa ataaatatat
     3961 agatcatacg cttttaaaac aagatgcaaa gaaaaaacaa attgatagtt tgttgtctga
     4021 ggctagagag tatgactttg ccagtgtttg cgttaatccg acctgggttg aacatgctaa
     4081 aaaaggactt gaaggcacag atgttaaggt ttgcacagta gtaggtttcc ctttgggagc
     4141 aacaacttca gccgtgaaag catttgagac aaaagaagct atccaaaatg gtgcagatga
     4201 gattgatatg gtgatcaatg ttggagctct caaatcaggt aatttagcct tggttgagtc
     4261 agatattcgc gcagtagtgg aagcaagtgg tgataagtta gtgaaagtca ttattgaagc
     4321 ttgccttctg acagaccaag aaaaaattgt tgtttgccaa ttggcccaaa aagctggggc
     4381 tgactttgtt aaaacatcta ctggcttttc aactggtggt gctacgatag cagatgttag
     4441 attaatgcgt gaaacagttg gatctgatat gggagtcaag gctgctggtg gagctcgttc
     4501 ttatgcagat gctcttgcct ttgtcgaagc aggtgcgacc cgtatcggaa cgtcagctgg
     4561 ggtagctatt ttaaaaggag aattggcaga tggcgactac tgagttgatt gaactggcaa
     4621 ttgaaaccag caaacatgcc tatgtcccct attctcactt tcctatcgga gcagttttag
     4681 tagccaaaga cggaagtgtt tacacgggag tgaatatcga aaatgctagc tatcctttga
     4741 ccaattgcgg tgagagaaca gctgttttta aggctatatc tgaaggccaa agagaatttt
     4801 cagaattgat tgtctatggt caaactgaaa aaccgatctc gccatgtggt gcttgtcgcc
     4861 aagtaatggt cgaatttttt gaacaagatt taaaagtgac cttagtcgca aaagataaat
     4921 cgacggtcga gatgacggtc ggggaattac ttccatactc ttttacagac ttaaactagt
     4981 ctgagtcgct ctctgagtgg cacggtcctt gtggccaacc aatccatact tgcaacatcg
     5041 ttgcacatct tatttaggag gttcagtaat gaacaagaaa caatggctag gccttggtct
     5101 agttgcagtg gcagcagttg gacttgctgc atgtggtaac cgctcttctc gtaacgcagc
     5161 ttcatcttct gatgtgaaga caaaagcagc aatcgtcact gatactggtg gtgttgatga
     5221 caaatcattc aaccaatcag cttgggaagg tttgcaggct tggggtaaag aacacaatct
     5281 ttcaaaagat aacggtttca cttacttcca atcaacaagt gaagctgact acgctaacaa
     5341 cttgcaacaa gcggctggaa gttacaacct aatctttggt gttggttttg cccttcataa
     5401 tgcagttgaa gaagcagcta aagaacacac tgacttgaac tatgtcttga ttgatgatgt
     5461 gattaaagac caaaagaatg ttgcgagcgt aactttcgct gataatgagt caggttacct
     5521 tgcaggtgtg gctgcagcaa aaacaactaa gacaaaacaa gttggttttg taggtggtat
     5581 cgaatctgaa gttatctctc gttttgaagc aggattcaag gctggtgttg cgtcagtaga
     5641 cccttctatc aaagtccaag ttgactacgc tggttcattt ggtgatgcag ctaaaggtaa
     5701 aacaattgca gccgcacaat acgcagccgg tgcagatatt gtttaccaag tagctggtgg
     5761 tacaggtgca ggtgtctttg cagaggcaaa atctctcaac gaaagccgtc ctgaaaatga
     5821 aaaagtttgg gttatcggtg ttgaccgtga ccaagaagca gaaggtaaat acacttctaa
     5881 agatggcaaa gaatcaaact ttgttcttgt atctactttg aaacaagttg gtacaactgt
     5941 aaaagatatt tctaacaagg cagaaaaagg agaattccct ggcggtcaag tgatcgttta
     6001 ctcattgaag gataaagggg ttgacttggc agtaacaaac ctttcagaag aaggtaaaaa
     6061 agctgtcgaa gatgcaaaag ctaaaatcct tgatggaagc gtaaaagttc ctgaaaaata
     6121 atggatggaa gtcatttctt aggaagcggc caagggccgc ttttttaaga gttgagacaa
     6181 agtcattttg tctcagtaaa gcttgctact agacaaacta gcaagtttta ttgaaataaa
     6241 ataagactct gaaaggaaga gcacatggca cacgaaaatg tcattgagat gcgtgatatt
     6301 accaaggtgt ttggtggatt tgttgccaac gacaaaatca acatgcacct acgaaaaggt
     6361 gaaattcatg cacttttagg agaaaatggg gctggtaagt ccacgctaat gaacatgtta
     6421 gcaggccttc ttgaaccaac tagtggtgaa atcgcggtca acggtcaagt tgtcaatctc
     6481 gactccccat ctaaagcagc tagcttggga atcgggatgg ttcaccagca ctttatgttg
     6541 gttgaagcct tcacagtggc tgaaaatatc attttaggta gtgaattgac taaaaatggt
     6601 gtgctagata tcgctggagc tagcaaagaa atcaaggctc tttctgaacg ttatggctta
     6661 gctgttgacc cttctgccaa ggtagcagat atctcagttg gagcccaaca acgtgtagaa
     6721 attttaaaaa cactttatcg gggggctgat atccttatct ttgacgaacc aacggctgtt
     6781 ttgactccat cagaaattga tgagttgatg gctattatga aaaatcttgt caaagaggga
     6841 aaatcaatta tcttgattac ccacaaattg gatgaaattc gagcagtttc tgaccgtgtt
     6901 acagttatcc gtcgtgggaa atcaattgaa accgttgaaa ttgcaggggc tatcaatgct
     6961 gatttggcgg aaatgatggt aggacgttct gtttccttta aaacagagaa gcaagcctct
     7021 aaaccaaaag aagtggtttt gtctatcaaa gatttggtgg tcaatgaaaa ccgtggtgtt
     7081 ccagctgtta aaaatctgtc cttggatgtt cgtgctggag agattgttgg tattgcgggg
     7141 attgatggaa atggtcagtc tgaactgatt caagccatta caggtcttcg taaggttgaa
     7201 tctggtagca ttgagctaaa aggagattca attgtaggct tgcacccacg tcagattaca
     7261 gaactaagtg ttgggcacgt tccagaagac cgtcaccgtg atggcttgat tttggaaatg
     7321 atgatatctg aaaatattgc ccttcaaacc tactataaag aaccacatag taaaaatgga
     7381 attttgaact attcaaatat tacttcttat gctaaaaagc tgatggaaga gtttgatgtt
     7441 cgcgctgcca gtgaattagt tcctgcagct gcactctcag gaggaaatca acaaaaagca
     7501 attattgctc gtgaaattga tcgagatcct gatctcctta tcgttagtca gccaactcgt
     7561 gggttggatg tcggtgccat tgagtatatc cacaaacgct tgattgaaga gcgtgataat
     7621 ggcaaggctg tccttgttgt cagctttgaa ttggatgaga ttttaaacgt ctcagaccgt
     7681 attgccgtta tccacgatgg taagattcaa ggtattgtat caccagaaac aaccaataaa
     7741 caagaacttg gtgtcttgat ggctggtgga aacttgggaa aggagaagag tgatgtctaa
     7801 aaaattacaa caaatttcgg ttcccttgat ttctgtattc ctaggaattt tactcggagc
     7861 cattgtcatg tggatcttcg gttatgatgc tatttggggc tacgaagaat tgttctatac
     7921 agcctttggc agtctgcgtg ggattggaga aatcttccgt gctatgggtc ctctggtctt
     7981 gattggtctt ggttttgccg ttgccagtcg agctggtttc tttaacgtcg gacttcctgg
     8041 tcaggctttg gcaggttgga ttctcagtgg ttggtttgcc ctgtcgcatc cagatatgcc
     8101 ccgtcccttg atgattctag caaccatcgt gattgccttg attgctggtg ggattgtcgg
     8161 agcgattcca ggtattctta gggcctatct agggacgtca gaggttattg taaccatcat
     8221 gatgaactac attgtcttgt atgtagggaa tgcctttatc catgctttcc ctaaagactt
     8281 catgcaaagt acagattcga ccattcgtgt tggggctaat gcaacctatc agacaccttg
     8341 gttgactgag ttgactggta actcacggat gaatattggt attttctttg ccatcattgc
     8401 cgttgcagtt atttggttca tgctcaagaa aacaactctt ggttttgaaa tccgtgcagt
     8461 tggtcttaat ccacatgctt cagaatatgc tggtatttct gccaagcgga ctattatcct
     8521 atctatgatt atttcaggtg ccttggcagg tcttggtgga gctgttgaag gtttgggaac
     8581 cttccagaac gtctatgttc aaggttcgtc attagctatc ggatttaacg gaatggcggt
     8641 tagtttgctt gcggccaact caccaattgg tatactcttt gcagccttcc tatttggcgt
     8701 tctccaagtt ggggctcctg gtatgaatgc ggcgcaggta ccatctgagc ttgtcagcat
     8761 tgtaacagcg tctattatct tctttgtcag tgttcattac cttatcgaac gctttgtcaa
     8821 accgaaaaaa caagttaaag gaggtaagta aagatgtcta ttataacctt gctccccctc
     8881 ttggtgtctt ctatgctgat ttactcagca cctctcatct ttacaagtat cggtggtgtt
     8941 ttctctgaac gtggtggtgt ggtaaacgtt ggccttgaag gaattatggt tatgggtgcc
     9001 ttttctggag ttgtctttaa ccttgaattt gcagaacaat ttggagcagc aactccatgg
     9061 ctatccttgc ttgtagcagg attggttggt agtgttttct ctatcatcca cgcagcagcg
     9121 acggttcatt tccgtgcaga ccatgttgtc agcggtacgg tattgaactt gatggcgcct
     9181 gccttggctg ttttcttagt taaagttctt tataacaaag gacaaaccga caacctaagt
     9241 caaacttttg gacgctttga tttcccagtc ttggcaaata tcccagtgat tggtgatatc
     9301 ttcttcaagt caactagtct acttggttat ctggcgattg ccttctcatt ccttgcttgg
     9361 tttattctct ttaagactca atttggtctt cgtctccgct ctgtcggtga acatcctcaa
     9421 gcagcggata ccttgggaat caacgtctac aagatgagat atttaggggt tattatttca
     9481 ggttttctag gtggaattgg cggagcgatt tatgcgcaat caatctcagt taacttctca
     9541 gtgacaacta ttgttggacc tggatttatc gcccttgctg cgatgatctt tgggaaatgg
     9601 aatccaatcg gtgctatgct ttctagtctc ttctttggac tttcacaaag tttggctgtt
     9661 atcggttctc aattgccgtt cctacaagga gtgccagcgg tttatcttca aattgcacct
     9721 tatgttttga caattcttgt cttagcagcc ttctttggaa aagcagtcgc accaaaagca
     9781 gatggtatca actacatcaa atcaaaataa gcatacaaaa aaacgtcagt ccaatgactg
     9841 aactacaccc ca
//
LOCUS       DAVLXF010000073         9734 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLXF010000073 DAVLXF010000000
VERSION     DAVLXF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.073"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(162..1925)
                     /locus_tag="VYO12_001658"
     CDS             complement(162..1925)
                     /locus_tag="VYO12_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1588226.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            complement(1934..2524)
                     /locus_tag="VYO12_001659"
     CDS             complement(1934..2524)
                     /locus_tag="VYO12_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1588227.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            complement(2622..3113)
                     /gene="rocS"
                     /locus_tag="VYO12_001660"
     CDS             complement(2622..3113)
                     /gene="rocS"
                     /locus_tag="VYO12_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1588228.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            complement(3190..3882)
                     /locus_tag="VYO12_001661"
     CDS             complement(3190..3882)
                     /locus_tag="VYO12_001661"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1588229.1"
                     /translation="MKIGIIAAMPEELAHLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            complement(3899..4213)
                     /locus_tag="VYO12_001662"
     CDS             complement(3899..4213)
                     /locus_tag="VYO12_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1588230.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            complement(4225..4770)
                     /locus_tag="VYO12_001663"
     CDS             complement(4225..4770)
                     /locus_tag="VYO12_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1588231.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            complement(4780..6159)
                     /gene="glmU"
                     /locus_tag="VYO12_001664"
     CDS             complement(4780..6159)
                     /gene="glmU"
                     /locus_tag="VYO12_001664"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1588232.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRCRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPRNQ"
     gene            6293..7093
                     /locus_tag="VYO12_001665"
     CDS             6293..7093
                     /locus_tag="VYO12_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1588233.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSIWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            7188..8030
                     /locus_tag="VYO12_001666"
     CDS             7188..8030
                     /locus_tag="VYO12_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1588234.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYSLNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNTGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            8030..8512
                     /locus_tag="VYO12_001667"
     CDS             8030..8512
                     /locus_tag="VYO12_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1588235.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            8512..9132
                     /locus_tag="VYO12_001668"
     CDS             8512..9132
                     /locus_tag="VYO12_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1588236.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPADRILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(9385..9480)
                     /locus_tag="VYO12_001669"
                     /pseudo
     CDS             complement(9385..9480)
                     /locus_tag="VYO12_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2969 a         2083 c         1692 g         2990 t
ORIGIN      
        1 ataagttata gtgtagcatt ccaacttcaa agcactataa agtaaattga aacaagaaca
       61 atacaaacaa ttctcgtaaa cggattgcaa ccacaaaaaa gcaagcattc acaagaatac
      121 ttacctatca tgggaggaac aaccgttcct cttttttatt actaaaattc aaagaattcc
      181 aatgcttttt tcaagagcaa atccgtatat tctggatctt cttgggctac ttctatttcc
      241 cgctgaactt tttccaaatc atctgtaatc actccatcta ctcctaagtg aagagatttg
      301 ctgatagctt ctgaatcatt gacagtccag acataaagtt tctgatccgt tgtccatagt
      361 ttgcttacaa aatattcatc caaggttgag tactccatag tatatcctgt cgctcttgtt
      421 ttaggaaaga cagaattgta gggcatgatg aaataaactg gcagttcggc atcatactgt
      481 cttacttttt cgacaacatg gtagtctaaa gactggattt gatgtccata aatcttgagc
      541 tttgcagcat aacgggctaa aaagcggttc atcatgtctg gactatcttt tttactggtt
      601 ttaatttcaa ttagtaattt ttgaccaagt tcgttggctc gactgagata atcttcaaag
      661 cttgaaattt tagtctggta gccattttca aaaatatcaa tccctttaag ctcctccaag
      721 tttaagtctt gaggactttt attgatacct gctagatttt tcaagttagc atcatgcatc
      781 atgacaaact gcccatcttt tgtttcctgc acgtccgtct ccaccaagtc tggtttgagt
      841 tgtgctgtag tttccaagga ctctactgta ttttgaatcc catttgcatt ggaaacccct
      901 cggtgagaaa taagttgagg tagatgaacc atgggagcct ccagataaat ataaccttct
      961 aaggcaaaga aaagactggc acaagtcatg acaccccatc gcacgatgtg atctttttct
     1021 ctcctaggaa gcatatccag ctcctttcct gtcaaaaatg aaacaaattt aaccaaaaaa
     1081 taagtcagag ccatataata gagattttta atcacgacaa aattcaaaat accaagaatc
     1141 agagactctc tctgagtgat atcatctacc aaagtttgag ccaataataa agggatcaaa
     1201 ggaagataaa ataataaatg tgctttgagc aagatgtaaa ataaattcca agcataaaaa
     1261 gtaactctct tcttggtttt ctccaagcta aacatcactg cttctcgaac agtcagctga
     1321 tcatatacaa tcttcggaag ggcaaacatc aatctgacag agacatagag aaagataaga
     1381 gatagaagta ggatgctcag ccaccacatc caatatctat cttctaaata agcttggata
     1441 aactctggaa tgacgatttt attaagataa taaatcttca gcattttccg tataaaagga
     1501 aacagcatag ctatatagaa aaagataaac aaggctttag cgcaagttag ctttttcata
     1561 aatccaaaac tttcatggaa aaccttgcgg atatactcaa ttagccttcg cttttcatta
     1621 tagaggagat gacgagcacc aataaagagg agtcctattt gaaaataagc aaccagaagg
     1681 ttaattacaa tcaaggctaa aaaagctaga ctaatcaatg gagaatgagt aaggatggct
     1741 aagacattgt tataggaaat aaaaagataa cctgtctgat ctaataagaa gctagccaac
     1801 catgaattga atggtaccca caaatactcc actatcataa aaatcaagaa aaatagaaag
     1861 aggattttat caagattgag gtaaatctgt ttaagaccca attttttagg tttttcaggt
     1921 ttcataggca ctcctagtca aataattgag acaagtccaa gccaccaaaa ggattgtttg
     1981 ataagctact ttctgtctct aacaattccc tagcttgatc cgactctaag aaggattcgt
     2041 aaacacgcgc cgtcatccga gcatcctcta aactattatg agactgacct tgaaatccaa
     2101 gaaatgaggc aacagtttgc aatttgagat tggcaatacc atgtaaatct gaactccgac
     2161 gttcaaaagc ttcatcatac aaatccacct tgtactgttg gctatagtct aaaccatgct
     2221 ctgctaaaat aggtaaatca cttttagcag cattgtagcc tatcatcagc aaagaaccca
     2281 caaactcttg gaattcttct aagactttct caactggagg agcatctttt aaggtctcag
     2341 ctgtaatccc ggtcaaacca ttgataaagc tttttagagg gacgctggta tgaacatagg
     2401 aatcataggc atcgatttcc cgaccatctc taaaacgcac tgccgatact tgaatcaagt
     2461 gtgtttgtcc ttcgtgctga ttaaattcta agtcaaaagc aatgtaatct tctaatcgtt
     2521 ccattctgta cctctcctct actgtctctg ttctaatcaa ctatactatt cacaaacaaa
     2581 agaagccatc aggttagcat ttaacctaaa cagctccttg attatcctcc aaataaacga
     2641 gcaaaaaagc cttttttagt ggattggact tcttcttttg cttggtccag ctctagctta
     2701 agattttctt gatccttcat ggcttgaagg gtcaattgtt gttgctgatc taattgtttg
     2761 tctttctcag caatctgacg gtctttgata cgcatctgct cgtctttttc tgataactga
     2821 cgatccttgg cttttaattg ttcataaaga cgaagaattt ctgcattctt ctcatccact
     2881 agaatctcca tcagttcacg ttgtttgaca tcttcactga caggttcatc ttcaaaaatc
     2941 gtttttttat agatttcttc tagcttaatc aagccacttc tggtaacaac tgttacccct
     3001 ttgtcatttt tatctgtgtc ttcttctggt aattctttga cacggttatt gattgcttgg
     3061 cgagataatc ctaagacctc tgcaatctca ctgacggtca tttcaatact cataatatcc
     3121 tctgaaacgt tttctagctt ttctttactt aaatcttatc ataagctaga aaaactgtca
     3181 aatttccgct taatctaaag ccttcaaaaa ggccaacaag acttgggcag agcgacgtcc
     3241 agcttcgata ataaactcat caaaaaagat gtttgcttca tggttggcat tgtcactcat
     3301 agctctgatg actaagactg ggagattgag ggcatgcgct gcttgagcaa tagctgcccc
     3361 ctccatctcc acggctaaaa cttctgggaa atgggactta atcgcttcta tcttgtcatt
     3421 tcctgcaaca aaactatctc ctgtagcaat caaaccaaga tgccagtttt ggtccaattg
     3481 agataaactc tcttggattt gagcaacaaa ggttttgtct gattcgaaat aaagcggttg
     3541 ttgcgccatt tgtccataag catagccaaa agctgtgaca tccacgtcat gataggctaa
     3601 tttgtcagca atcacgacat ccccaacagc gataccttct gctactgccc cagctgaacc
     3661 cgtattaata agggcatcca cctggaaatg atcagccaaa atcgccacac tcatagcaga
     3721 catgacctta ccaattccac tttctacaag aacgacttca tgagaagcaa tggttcctgt
     3781 atgataggta ttccccaaaa caacttgctc ctgggcattg tctaaatgct ggaccagatg
     3841 agccagttct tctggcatag cagcaataat tcctattttc atttcaattc cttttctatt
     3901 acaaaagttt cattgctaaa acaagcaaaa tcaagagaaa gatgaccgcg aataagattt
     3961 tattcaactt agaattgaag acatttctct tggtattttc aatgcgacgg cttttatgaa
     4021 tagatggttc gacctgaatc ttcaaggttt cctggctaaa accatgatcc ttaggattgg
     4081 cataaccaaa acgggaagaa gaggttggta aaatcttagt ttcctcatta tcatccagca
     4141 aaggaggacc tgaaattttt tcgcctctat tagctctttc aatcatttca tccgttaata
     4201 aagatttacc catactgacc tcctctattt tttctgcaac tcccaatact gaacagccat
     4261 aattgtcttg gcatcacaga tatgacctga ttggattaat tctttcgctt cttctaagct
     4321 cacttcaagg acttccaagg tttcatcctc atcctgcgga cgcggatttt ccacttttgt
     4381 caaatcgctt gctaaatata gttttaactt ctcattacaa aagccaatag ctgaataaaa
     4441 atcgtacaag agttctaatt tccctgtata ggctgtttct tcctctaatt cacgaagggc
     4501 agctgccaca ggggctgtgt tttctcctac ctccaatttt ccggctggaa tttcgtaaga
     4561 gacagcctcg atagctttgc ggtactgctt gaccaagata agtttttgtt catccgttac
     4621 tgctaaaaca cagacagccc cattgtggaa aatcaaatcc cgttgggcag ttcccttgcc
     4681 ttctggtaat tcaacctgat cttggaccag tttaaatatt ggtccttgat agatttcttt
     4741 tcggctaagc gttttttctt caaactccat gataggctcc tactggttcc taggatgatg
     4801 aggaagacgt gttgcatatt cgtctttatt gatctgacga ccgcgaccaa tagcaatagc
     4861 atctgctggc acgtctttag taatagttga accagcacca acgagggaat tgtcaccaag
     4921 ttctactggt gcaataatgg ttgaatttga accaacaaag acattgtctc caatgactgt
     4981 cttgtatttg tttttgccgt catagttgac tgtaatagtt ccagcaccga aattaacgtt
     5041 gcttcccact tcacagtttc cgatataagt caaatgacca gccttggtat tctcaccgat
     5101 tgaagatcct ttcacctcaa caaagttacc aatatgaact tgggcaccca gacttgaatt
     5161 tggacgaatg tgagcataag gaccgactgt cacaccgtct gcaacactac tttcctcaat
     5221 catagaattg gtaatgaccg ctcctgctcc gatagtgctg tccactacat aagtaccgtt
     5281 tgtcaaaaca gtctcagcac caattttcgt ttgccctttc aaggtaacat tggcttcgat
     5341 ttgaacttcc ggagcaatct caacatcaat atcgatataa gttgcttctg gattgacaaa
     5401 gctaacacca ttgaccatgt gtttatgatt gatgcgacaa cgcataactg actcagctgt
     5461 cgcaagcgcc acacggtcat ttaccccaag actttcatca aaatctttca aagtataagc
     5521 gccaactttt tcaccagttt cacggaaaat accaatgacg tctgtaatat agtattcgcc
     5581 ttgagcgtta ttggtattga tatttttcaa agcctcaaac aaacgctcgt tgtcaaagac
     5641 gtatgttcca gtgttgattt ccttgatttg cttttcaaaa tctgtagcat ccttctgctc
     5701 aacaatacga agaacctcag cattgtcatt acgaacaatt cgtccatagc caaaaggatt
     5761 atccgtttca gcagtcaaga tagtggccac atttttatga ttgatatgga aatcaatcaa
     5821 gtttttcaag ctttcaccag tgattaaagg agtatctcct gcaatgacca aggtgtgtcc
     5881 tgacaaacct tctaagatag gctctgtcat cataactgca tgaccagttc ccaactgttc
     5941 agattgagtc acaaattctg tctgtccagc caagacctcc tcaaccaatt ctgccttgtg
     6001 tcctacaact gttactgtct tttcaggttg gatagctccc acactacgga aaacatgttc
     6061 caacatagaa atacccgcaa ccttgtgcaa aacttttggc aaatcagatt tcatgcgagt
     6121 ccctttaccc gctgctaaaa taatggcaaa atttgacata atcttctctt ttctgtagaa
     6181 attccctttt attataccat atttcaaatc attccgcact cttgaatgaa aaaatgctct
     6241 aaagtctctt ccttaaccca tttttttgat ttttagggta gaattataag atatgagaaa
     6301 acaaattcat ccacttattt tatttagttt tttggggatt atgatgttca ttttaatcat
     6361 caatcgccca cttaatgaca accaatcgtt taaaacaaaa tccaatatag cacagattga
     6421 agcacaggct ttgaaacact tagacaaacc gattattgac ctctctggct ggcagcgtcc
     6481 tgaggaaatc aactacgatg ccctctcaca aaatatttca ggtgctattg ttcgtgttca
     6541 cagtggtgct caaacgacaa aagaaaatga tgcttcattt atcaatggaa tagacaaggc
     6601 ctataagagt catatcactg aacttcaaaa gcggaatgtc ccagttgctg tctacgctta
     6661 tgtagctgga aaaagtgtcc aagaaatgga aaaggctgcc gaagttttct acaatgctgc
     6721 ctctccttac agccctagtt actattggct tgacgtggaa gacaaaacca tgtctaatat
     6781 gaacgaaggt gtcgaaaact ttcgagcaaa actagcatca ctaggtgcta aaaacatcgg
     6841 catctacgtt ggtgtctact tcatggaaga acacagtatt gatacaggca agtttacctc
     6901 tatttggatt ccgtcctacg gctcagactc aggatttttg gagagcagtc ctaaaactga
     6961 tttagattat gatattcacc aatacacatc taaaggaaaa attgccggct ttgaccacga
     7021 tttagatatc aacgttatct ctcccttaaa aaataaagaa gaaacctttc gaaaactttt
     7081 tttaaaacct taaaagcatt tgtttatcat ttgctttttc tttttgtgtc tgattgtgat
     7141 acaatatagt aaggattttt tgaaatagaa atagttggag gaaatatatg ctctcatggt
     7201 tagcacgcgt tattaaagga attgtgattg ctcttggatt tatcctaccg ggaatttccg
     7261 gaggggttct agcagcaatc ttaggaatct atgaacgaat gattggcttt ctggctcatc
     7321 cctttaaaga ccttaaagaa aatgttttgt actttattcc agttgccatc ggtatgcttc
     7381 tgggaatcgg cttattttcc tacccgattg aatacctgct tgaaaattat caggtctttg
     7441 tattatggag ctttgcggga gctattatcg gtacagttcc tagcctcctc aaagaatcaa
     7501 ctcgagaatc tgaccgagac aagattgatt tagcttggtt atggacaacc tttatcattt
     7561 ctggattagg actctattcc ttaaattttg tcgttggaac cttaagcgcc agctttctta
     7621 acttcgtcct agcaggcgca ctattggccc ttggcgtctt ggttcctggc ctcagcccat
     7681 cgaatttact tttgattttg ggactctatg ctcctatgtt gactggtttt aaaacttttg
     7741 atctcttggg aaccttcttt ccgattggaa ttggtgcagg tgcaactctc atcgtttttt
     7801 caaaattgat ggattatgcc ttaaacaact accactcacg cgtctatcat ttcatcatcg
     7861 gtatcgtcct atcaagtacc cttttgatct taattccaaa tacaggaaac gctgaaagta
     7921 tccaatacac aggactttca cttgtcggtt atgtcatcat cgccttcttc tttgcgctgg
     7981 gaatctggct tggtatttgg atgagtcaat tggaggataa atataaataa tggcaaaaaa
     8041 agttaaaatc aaaaaaacat tggtggaaca aatcctatct aaagcagcta tccctcatca
     8101 ggggattcaa atcaatgccc tagaaggaga gcttcctcaa ggttatgaac gagatcagat
     8161 tttcaaaacc ttggcgcttt tgggagacaa gaccggaccg attatcggaa ttgtccctat
     8221 cactcagcac ttgtctgaaa agaaactagc taaaatttct ggcaataaaa aagtgagcat
     8281 gattccacaa aaggacttag aaaaaacaac tggttacatt catggagcca ataatcctgt
     8341 cggaattcgt cagaaacaca attatcccat ttttatcgat aaaattgctc tagatttgga
     8401 tcgaatgatt gtctctgcag gcgaagtcgg acacagcatt atcgtcgcgc cacaagactt
     8461 ggctagcttt gtaaaggcag actttgtaga tattttggag gacatcaagt aatgaaactc
     8521 tactttgtcc gccacggtcg taccctctgg aaccaagaag gtcgttttca aggtgctagc
     8581 ggtgattctc ctcttcttcc tgaatccatt gaaaccctaa aacgacttgg ccagtatctc
     8641 aaggaaattc cttttgatca gatttattca agtgatttac ctcgagcggt caaatctgct
     8701 gagattatcc aaagtcaact ccataccccc tgttctttag aaatcgttcc taatctccgt
     8761 gaatggcaac ttgggaagtt agaaggtttg aaaattgcaa ccttggaagc tatttacccg
     8821 caacaaatcc aggcttttcg ttcaaatctt gctcaatttg acactcgaat gttcggagcc
     8881 gaatccctct atagtacaac tcagcggacc atccaattta tcaaatcatt aaaagatagt
     8941 ccagctgatc gtattctaat tgtcggacac ggcgccaatc ttactgccag tcttcgtact
     9001 ctcctaggct ataaagaacc acttcttcgt aaagatggcg gtctagcaaa tgccagcctg
     9061 accattatag aaacccatga ttttgaaaca ttcactctca atacttggaa tgatacttct
     9121 tatcaatcat aattatagcc tagccttctg aactagaacc ataggagata tagtcagttg
     9181 aaacaaaaat aagacaaaag aaccttgtaa aaaggtattg caacttgcca ataccttttt
     9241 gatatgaaca cgtgtcttct caacaggatt gtagagcaaa atgaaataag aactgaataa
     9301 tatgatttga gaatttaaat caatttataa gaatgtttta gaagcagtta tgtactattt
     9361 tagattcact tttctatact aagatgagta gggaggaaaa ggtaaaagtt tatgcccaaa
     9421 ttcttcacat aagaactcta gcttacccat tctatggaat cttgcattat ccattaaaac
     9481 ttccagactc atacccttct aaaatctatt caaaccacgt cagcttcaca ttatatatgt
     9541 tggtgacctc acaagtttta tctacaacct caaagctgtt ctttgagcag cctacagcta
     9601 gcttcctagt ttaatctttg cttttcactg agtatcaagt taaaaaaatg aggctggtca
     9661 aaaagtcttt aaaataaaaa acgcatatta tcaggtgtta aatgaactgc accccaaaag
     9721 ttagacagaa aaaa
//
LOCUS       DAVLXF010000074         9516 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLXF010000074 DAVLXF010000000
VERSION     DAVLXF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.074"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            129..1622
                     /locus_tag="VYO12_001670"
     CDS             129..1622
                     /locus_tag="VYO12_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1588237.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..2744
                     /locus_tag="VYO12_001671"
     CDS             1635..2744
                     /locus_tag="VYO12_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1588238.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLA
                     LQNEFADFVVQVDKSQFACEIAIKVWRNSLKFSII"
     gene            2774..6059
                     /locus_tag="VYO12_001672"
                     /pseudo
     CDS             2774..6059
                     /locus_tag="VYO12_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            6303..6404
                     /locus_tag="VYO12_001673"
                     /pseudo
     CDS             6303..6404
                     /locus_tag="VYO12_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6645..7115)
                     /locus_tag="VYO12_001674"
     CDS             complement(6645..7115)
                     /locus_tag="VYO12_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1588239.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(7132..9405)
                     /locus_tag="VYO12_001675"
     CDS             complement(7132..9405)
                     /locus_tag="VYO12_001675"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1588240.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSSQDVIREVVYVESRVDTDQKGLPDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWYDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         3048 a         1560 c         1999 g         2909 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttctaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgttt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaaaggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taaacttgct cgtcaaatcc cgatttaacg
     2161 agatgtttgg ggaaaataaa atatttgaaa gcattgataa cttatttgat attatagatg
     2221 gtgatagggg caaaaattat cctaaatcag atgagttgtt tagtgaggag tactgtttat
     2281 ttttaaatac aaagaatgtt actaaaaacg gattttcatt cgatacaaag caatttatca
     2341 ctaaaacaaa ggataaatta cttcgaaaag gcaaacttga gcgttatgat atagtcttga
     2401 caacaagagg tactgttgga aatgtagcgt actacgatga attaataaaa tataaacatt
     2461 tacgtataaa ttcaggtatg gtaatattac gtcccaagac accaaatcta aatcagaaat
     2521 ttattatcca tgttttaagg aataataatt atagtcgagt gatatcagga agtgctcagc
     2581 ctcagttacc aattacaaaa ttaaaaaaaa tacttctccc cctcccccca ctagccctcc
     2641 aaaatgagtt cgcagacttt gtagtccagg tcgacaaatc acaatttgct tgtgagatag
     2701 ctataaaagt gtggagaaat agcttgaaat ttagtataat atagctaaac tatttattta
     2761 aagtgagaaa aaaatgggaa attttagctt tcttttaaaa aatgacgaat atgaatcttt
     2821 ttcaaaacct tgcattgaag ctgagaatat gattgctaca tcaactgtgg ctactgcctt
     2881 tatggcgcgt cgtgctttag agcaggctgt ccattggata tatagtcacg attcatattt
     2941 agaagctccc tatcgtgcta ctctatcttc tttagtatgg gatgatgatt ttagggatat
     3001 cgtagattct gaactccaca agcagatagt tctgttgatt cggtggggaa accatgctgc
     3061 tcatggtggt gaaattaagg aacgagaagc gattttagct ttgcatcatt tgtatcagtt
     3121 tgttaatttt atcgattatt gttacagcaa tgagtttgtg gagcgttatt ttgatgagaa
     3181 gtgcttacca ctttcagcaa acatcaaata ccgagaaact ccacaatcta tgataaagtt
     3241 acaagacagt ttaccagaac tgcctgattt tcatgaacag atggctgctc agtccgtaga
     3301 agttcaagag acttatactg aaaaacgtga gactgcagcg caacggcaag atgtgccttt
     3361 ccatattgat caattatctg aggcagagac aagaaagctc tttattgata tcgatctccg
     3421 tttagcagga tggatatttg aagaaaactg tcgtgttgag atagccgttg atggtctcaa
     3481 gcacggttca ggaattggtt actgtgacta tgtactttat ggtaaaaatg ggaaaatttt
     3541 agcgattgtg gaggctaaaa aagcctctgt caatccagaa gtaggggaag tacaggtcaa
     3601 agaatatgct gaagctttgg agaaacatat cggctatcag ccaatttgct ttattacaaa
     3661 tgggttgaag cactatatac ttgatggtcc gaaccgccgc cagattgcag gcttttactc
     3721 tcaagaagaa ttgcaattag tgatggatag acgtcatctt caaaaaccgc ttgaggatat
     3781 ttctagtaaa attagggacg atatttccgg gcgtcactac caaaaacatg ccattgcaag
     3841 cgtttgtgaa gctttctctg atcatcgtag acaggcactt ttggttatgg caactggggc
     3901 ggggaaaact cgtacagcag tttctctagt tgatatctta tcacgtcata actgggtaaa
     3961 aaacgttctc ttcttagccg atagaacttc cttggttaag caagcctatg attcgtttag
     4021 aaaattactc ccagatcttt ccgtttgtaa cttcttagaa gataaagaag gagctcaatc
     4081 aagtcgtatg gtcttttcaa cttatccgac catgattgga gcgattagtg gtcaagaaga
     4141 agtaaatcaa cgccctttta ctgttgggca ttttgacctt atcataattg acgaatctca
     4201 ccgttctatt tatcagaaat acaagtccat ttttgattat tttgatgcaa ggattgtagg
     4261 cttaacagct actccgcgtc aagatttaga taaaaacacc tatggattct ttaatttgga
     4321 gaatggggtt ccaacatatg catatgattt ggaagaggct gttaaagacg gatatttagt
     4381 agcctatcat tctatcgaaa ccaaactgaa gctacctacg gatggtctac attatgatga
     4441 tttgtccgaa gaagaaaagg aacattttga tagcaaattt gaagacgata gctgtgaaaa
     4501 agatattgat gggagtgtgt ttaattcctt tattttcaat aaaagtacag tagaaattgt
     4561 tttaaatgaa ctcatgacaa gaggaattca gacagcctcg ggtgatgaaa ttggtaaaac
     4621 tattattttt gctaaaaatc atgaccatgc ggaatatatc agaggtattt ttaacaaccg
     4681 ctatcctgaa aaagggagcg actatgctca ggtgattgat tatagtatta agcattatca
     4741 gaccttgatt gatgatttta aaattaagga gaagtatcct caaattgcga tttctgtcga
     4801 tatgttagat acaggtattg atgtaccaga ggttgttaat ttagtcttct tcaagaaagt
     4861 acgctctaaa actaagtttt ggcagatgat tggtcgagga acccgtctat gtaaagattt
     4921 atttggacct gagcaggata agaaaaactt cttggtattt gattatgggg acaattttga
     4981 ttattttcgt gcagatccaa gagatggaga gggtcgtcac attgtttcgc tgactcagcg
     5041 tttatttaat atcaaagtgg acttgattcg agaacttcag ggactccaat accaagaaga
     5101 tcagtttgcg agagcatacc gtcagcagct tgtctcggaa cttcaaggtc gtatagagag
     5161 cttaaatgag ttggacttca gggttcgtat ggttttagat acagtttata gctataggaa
     5221 attggaaagt tggcagaatc taactgctgt tacaagtgaa accattcaaa aaaatctctc
     5281 tccgctttta tttgatgaag ataaagaaga tgagatggcg aggagatttg atttgtggtt
     5341 gcttcatatt cagttggggc aactgacagc taaatcttcc actgttcata tttcccaagt
     5401 gatgaagacg gctagagctc tttctgctat tggcaatatc ccgcaggttt ttgagcaggc
     5461 tgaaattatc agggaagtac aggagcctga attttggaaa gaagttaact tgtctgattt
     5521 ggaaaaaatt cgtcttgcta ttcgagattt attacagttt ttggataaaa cagaccgtaa
     5581 accctactat gttaactttg aagatcgtat actctccact gttcacgaga ccacagcatt
     5641 tttgcaggtc aacgatcttc ggtcttacaa tgaaaaagtt gacatctgat gatatgcttg
     5701 cacttgaaaa attgctttgg gaaaaattag gtagtaaagc agactaccaa agtcattatg
     5761 aaaataaggc aattccgaga ttggttcgtg agattattgg cttagataga gagtctgcca
     5821 atcgtatttt ttctaaattt ttgtcggatg agaatcttaa tgccaggcag atttcatttg
     5881 taaaattgat tgtagactac attgtagaaa atggtttttt agagacgaaa gtgttaacgc
     5941 aagagccgtt taaatcttat ggttctgttc aactactctt ccaacaccaa ctaccagtac
     6001 ttcgtaatat tgttcaaatc attgaactta tcaataatcg agctggagaa gcggcttaaa
     6061 ttctaaagtg attgccatgc tgagactcat ttaaaattaa aaagagtaga aatttatgat
     6121 atatatgaga agttttatta ggaagaatgt catcgttttt ctagaataca gtatcagttg
     6181 ttaagtggct gataaatttc aaagtagata cttgtaccac gatgtttgtt gatcgagtta
     6241 ttaacaaaag agctactttg attttaaaga aatagaaaac aaaaagccga gcaagaattc
     6301 aattgcagga gaaaatgaaa taatactcaa tgaaaatcaa agagcaaact aggaaactag
     6361 ctgcaggctg ctcaaaacac tgttttgagg ttgtagataa gactgacgaa gtcagctcaa
     6421 aacatggttt tgaggttgta gatgaaactg acgaagtcag taaccatacc tacggcaagg
     6481 cgacgttgac gcggattgaa gagattttcg aagagtataa atcttcctag gataaagcaa
     6541 aacgcatagt atcaagggtt ttcaacactt gatactatgc gttttctgat gttaaagact
     6601 ttctaccagt ttttttaagc ataattgtta gttgtagtca tttattattc ttcaaagaaa
     6661 aatggtgggg cgaatttttt cagttcttca aagcactttt gagcagtatc tgcatcttca
     6721 cagatgataa gacagacatc attaccacaa agggtagcga tagcgtcagg gaagctcaaa
     6781 gtatcaatga tagaaccaaa ggattgagcc agtccaggaa gggtttttag taggacttgg
     6841 tgttgaactg ggcgcatcca gacaagggcg tcttccatgt agagttcgag acgtttttcc
     6901 cattttgaga tggaaccatt gttaagaaca taataagcgc tatcttcttc gcggactttt
     6961 gataggttca tatttttgat gtcgcgtgag agggttgcct gggttacttg aatgtcgttc
     7021 ttagcaagaa gggcttgcaa ctcagcctgt gtatgaatct tgttttttgt gataagagcg
     7081 cgtataagtt ggtggcggtg ttctgattta ttcataataa tgtaactcct tttagcaagg
     7141 taaggtaagc atggactgag cgaggtcgac agtcaagtgg tagtctgtat tgtcacggat
     7201 ggtgatttca aagtcagtag tatagaggac taaacggaga gtgtctcctt cttttagctt
     7261 gtaaatagtt ggctgcagtt caaattgaac gtccatccat tcatctgcag taatatcctc
     7321 tactaacagt aaatcatttc tattttgtaa attaaggtaa ccttttgtca cgactcgttg
     7381 tgcctctggt ctaaatggca attcacagag attttccaac atgtgatagc gaccgttgtc
     7441 aatggttcta gcacttaaaa tagctggata aggttgtagg tatttctttt gcccaaattc
     7501 tagcagttgg gcagataaga gccccttgtt tgtactggat ttgatacgaa gattgagctg
     7561 agcgcgacca tttaggtgga gatctttagt cacaggaagg ttaatagtaa tctgattggc
     7621 tttcccttga tagagctctg tattgaaggt ttggtatgtc ttaccatagc gctcaaaatc
     7681 cttatctggg tactggtttt gaatagcttg ctcttcttga ccaagtgaga aggtttcaca
     7741 gttttcttgc ccaccgaagt tatcaagtga taaccaagtc tgtggagctg tattgtcctg
     7801 ccagataaca gtaggaagtt gaaagtctgt ttcctgtcct agtaatttct tggtcaataa
     7861 ggcatttatg gactcacgga agtcaattga ttgccaattg ttcatgtaaa catgagcacc
     7921 attatggaaa aagagatgct tgtgtatatg agtaggaaga gcatggaaca tctggtaaac
     7981 atgaagtggt ttgacattcc aatcctgaga accatgagta aagacaacct ctgcctttac
     8041 tttatgggca ttgagcagat aattgcggtc ataccaaaac tgattgtagt ccccagtttt
     8101 tcgatctagc tgagctttca ctttttctaa gtcagcttgg tgagcttcat tgccacggat
     8161 atagtcgcca gctaagagat tacgagaata ggttaactca gcaagggagt caaagtcctc
     8221 acctggataa ccacctgggc tagtcaccag accgttttca cggtagtagt tgtaccatga
     8281 tgaaattcct gcctcggcaa tgataacttc taaaccatcg actcctgtag tcgcaagacc
     8341 attggacatg gtacctagat aggaaagtcc tgttgtagca acttttccgt ttgaccaatc
     8401 agccttgact tgacgctggc gcgtgtgatc agtaaaggca cggcaacggc cgttaagcca
     8461 atcgatgaca tttttataag cctcgatttg ctggtagtct ccattagtca tgaaacctgt
     8521 cgagtctttg gtaccaacac ctgagacata gagattggca aagcctctcg gaaggaagta
     8581 gtcgtttagt gtatagctag agttgatgtg agttagcttt tcctcagcct ctgctataag
     8641 ctcagcttta ccttggggtt ggacgagatt tagttgaggt ttctctagct caatcttgtg
     8701 aggaagctta acctcaagct cgccctccat cttgtagaga gccttgtcac tagccttgtc
     8761 attggttccc tgatgataag ggctggctgt catgatggca gggatttttc catcaaaacg
     8821 agggcgaata atgctaacct ttactaggtc tggtagccct ttttggtcag tatcgacacg
     8881 agactcaacg taaacgactt cacgaatgac atcctggcta gaaaaagtag ccaaactctt
     8941 gccgttaaag tagtggtagt cattatcctc cggaataaga ccatcactaa caagttggtc
     9001 gataagagta tttccttttt tggtgcgagt atttagtaac tgatagagat tttcaatcaa
     9061 gtcaccatat ataatgggaa atccagtttc tttacgaaaa acgtcactat cttcgaagtc
     9121 aaccaaataa gaaaagccta aaagttgaaa agcaacagta taaaaaatat ctgctgtcag
     9181 ttcatcttct gattgaaaaa atgtcagcag gtctgttttt ttatcagctg ctaggataga
     9241 aagtgggtag ttggtgtctt gataagtgaa aaagaaacga cgtaaaaagg tttcaagtga
     9301 gtctttgtga ttggctgtat tttgtaaatc aaagccacat ttttttagtt cagataagac
     9361 attttctttt ggaaaattga tataactata ttgattaaaa cgcatagaac ctccatatag
     9421 aatgacagtt aaggttatta tatcaaaaaa aaagcagaaa gggaattgtt aacttcaaaa
     9481 ggaaataatc caataaaaat gaataaagta ctaaat
//
LOCUS       DAVLXF010000075         9304 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLXF010000075 DAVLXF010000000
VERSION     DAVLXF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.075"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            480..1523
                     /locus_tag="VYO12_001676"
     CDS             480..1523
                     /locus_tag="VYO12_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1588241.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1690..3777
                     /gene="topA"
                     /locus_tag="VYO12_001677"
     CDS             1690..3777
                     /gene="topA"
                     /locus_tag="VYO12_001677"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1588242.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMAVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3883..4242
                     /locus_tag="VYO12_001678"
     CDS             3883..4242
                     /locus_tag="VYO12_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1588243.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4335..4967
                     /locus_tag="VYO12_001679"
     CDS             4335..4967
                     /locus_tag="VYO12_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1588244.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            5011..5667
                     /locus_tag="VYO12_001680"
     CDS             5011..5667
                     /locus_tag="VYO12_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1588245.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5686..6780
                     /locus_tag="VYO12_001681"
     CDS             5686..6780
                     /locus_tag="VYO12_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1588246.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGIAVANSLAAVKNGAGRVEETINGIRERAGNAALEEIAVALNIRQDYYQ
                     VETSIVLTETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQERVLVTVENRDTETILNAAGLDFDVLKASA
                     IAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6792..7818
                     /gene="leuB"
                     /locus_tag="VYO12_001682"
                     /pseudo
     CDS             6792..7818
                     /gene="leuB"
                     /locus_tag="VYO12_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            7815..8084
                     /locus_tag="VYO12_001683"
     CDS             7815..8084
                     /locus_tag="VYO12_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1588247.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8088..8447
                     /gene="leuD"
                     /locus_tag="VYO12_001684"
     CDS             8088..8447
                     /gene="leuD"
                     /locus_tag="VYO12_001684"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1588248.1"
                     /translation="MVGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8609..9232
                     /locus_tag="VYO12_001685"
                     /pseudo
     CDS             8609..9232
                     /locus_tag="VYO12_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2884 a         1636 c         2062 g         2722 t
ORIGIN      
        1 actgttatag attcaataca ctataattta gtgtaattga gaaaggagaa atgattgtga
       61 ttgatgttgg ctaggttatg ttcaatgatt cctaccgtct caaatcttgt cagtaaggaa
      121 aaataaattc ttcaaaagta gagattacaa ggcttgttta agaaagaatt caaagacctt
      181 gacaaataaa aataaaatgg ttattataag aaatggtcta aaatagatga tgatactttt
      241 cgaaaatctt tttacgtcag ctcagctttg ccttgctgtg ttttgagcaa gctacggtta
      301 gcttccgagt ttgattttca tttactagaa atgaaactga tgagagatat cagtagacat
      361 ttgagtcagg atattatgga aaatgataaa aagagctcgt gagattggca tatcagacta
      421 ctaaagtatt gagtttgtta ggattttagc gactagttag ctgggaaagg aagatatttg
      481 tgacaaataa taaactgtat tcgttgatag aatttagaaa taaaatatat gaagaattag
      541 aactttccag aagtgattta gcgattttac tatgtgccat gcttatcgcc tctatcggat
      601 taaatatgga ttcgactccc gtgattattg gagccatgtt aatctctcct ttgatgacac
      661 ctattctggg agtggggctc tctctagcta tatttgattt taaattgtta agaaaatctt
      721 ttaaaatatt agctattcaa attcttgcca gtctaatagc ttcaacactt tatttttatc
      781 tttctcccat ttcgtatgct agttcggaga ttgttgctag aacctctccg actatttggg
      841 atgttctcat tgcttttgta ggagggatag caggtattat tggtgctagg aaaaaagaga
      901 ccaataatat tgttcctggt gttgctattg caaccgcctt gatgcctcct ctttgtacag
      961 taggttatgc tattgcttct gctaatctaa aatttatcat aggctcctct tacctattcc
     1021 tcatcaattg tagctttatt gtcattgcga cttatatagg tgttaggttg atgatggtta
     1081 agaaacatta ttttaaagat aacgaagaag actctaaaat gcgtaggatt ttgcttctag
     1141 ttgctgtttt gctgatgatt ccgagtttca tctctgcaac gactttagtg agagaaacgt
     1201 tgaaaaaaga gtcccttaag aaatttatat cagagcagtt tcaggggtat aatattttga
     1261 aaaaaaccta ttctaaaaag actcataccc taaagctaac catttcagga aattatttga
     1321 cagaagaaga actcgatatg atttcaagta agagaggtga ctatggttta agtgatgttt
     1381 ctgttcaagt ttcacaattg tctgattcag aacaacttag taaggaagag ctggtggagt
     1441 atttcttcca gtatatcaag gataaggaag caaaagaaaa ggagaaagcc aataaatttg
     1501 atacagagtc tgaggagcaa taatttcttg agaatagctg gtttctctcg tgagtcttct
     1561 atgtatatca aaggaagact gaggttttaa gtataaaact tttcttctat tatagtagct
     1621 aagtcttgac agggttcaaa aaatggttta cactttataa agtttattac tttgaaaagg
     1681 tgtgatactg tggctacggc aacaaaaaag aaaaaatcaa cagttaaaaa aaatctagtc
     1741 atcgtggagt cgcctgctaa ggccaagacg attgaaaaat atctaggcag aaactacaag
     1801 gttttagcca gtgtcgggca tatccgtgat ttgaagaaat ccagtatgtc cgtcgatatt
     1861 gaaaataatt atgaaccgca atatatcaat atccgaggaa aaggccctct tatcaatgac
     1921 ttgaaaaaag aagctaaaaa agctaataaa gtttttctcg cgagtgaccc ggaccgtgaa
     1981 ggagaagcga tttcttggca tttggcccat attctcaact tggatgaaaa tgatgccaac
     2041 cgtgtggtct tcaatgaaat caccaaggat gcagtcaaaa atgcttttaa agaacctcgt
     2101 aagatcgata tggacttggt cgatgcccaa caagctcgtc ggatcttgga tcgcttggta
     2161 gggtattcga tttcgcctat tttgtggaag aaggtcaaga agggcttgtc agcaggtcgc
     2221 gttcagtcca ttgcccttaa actcatcatt gaccgtgaaa atgaaatcaa tgccttccag
     2281 ccagaagaat actggacagt tgatgctgtc tttaaaaagg gaaccaaaca atttcatgct
     2341 tccttctatg gagtagatgg taaaaagatg aaactgacca gcaataacga agtcaaggaa
     2401 gtcttgtctc gtctgacgag taaagacttt tcagtagatc aggtggataa gaaagagcgt
     2461 aagcgcaatg ctcctttacc ctataccact tcatctatgc agatggatgc tgccaataaa
     2521 atcaatttcc gtactcgaaa aaccatgatg gttgcccaac agctctatga aggaattaat
     2581 atcggttctg gtgttcaagg tttgattacc tatatgcgta ccgattcgac tcgtatcagt
     2641 cctgtagcgc aaaatgaggc ggcaagcttc attacggatc gttttggtag caagtattct
     2701 aagcacggta gcaaggtcaa aaacgcatca ggtgctcagg atgcccatga ggctattcgt
     2761 ccgtcgagtg tctttaatac accagaaagc atcgctaagt atctggacaa ggatcagctc
     2821 aagctatata cccttatctg gaatcgtttt gtggctagcc agatgacagc ggccgttttt
     2881 gataccatgg ctgttaaatt gtctcaaaat ggagttcaat ttgctgccaa tggtagtcag
     2941 gttaagtttg atggttatct tgccatttat aatgattctg acaagaataa aatgctacca
     3001 gatatggctg ttggagatgt ggtcaaacag gttaatagca aaccagagca acatttcacc
     3061 caaccgcctg cccgttattc tgaagcaaca ctgattaaaa ccttagagga aaatggggtt
     3121 ggacgtccat caacctatgc gccaaccatt gaaaccattc agaaacgtta ttatgttcgc
     3181 ctggcagcca aacgttttga accgacagag ttgggagaaa ttgtcaataa gctcatcgtt
     3241 gaatatttcc cagatatcgt aaacgtgacc ttcacagctg aaatggaagg taaactggat
     3301 gatgtcgaag ttggaaaaga gcagtggcga cgggtcattg atgcctttta caaaccattc
     3361 tctaaagaag ttgccaaggc tgaagaagaa atggaaaaaa tccagattaa ggatgaacca
     3421 gctggatttg actgtgaagt gtgtggcagt ccaatggtca ttaaacttgg tcgttttggt
     3481 aaattctacg cttgtagcaa tttcccagat tgccgtcata cccaagcaat cgtgaaagag
     3541 attggtgttg agtgtccaag ctgtcatcag ggacaaatta ttgagcgaaa aaccaagcgt
     3601 aatcgccttt tctatggttg caatcgctat ccagaatgtg aatttacctc ttgggacaag
     3661 cctgttggtc gtgactgtcc aaaatgtggc aacttcctca tggagaaaaa agtccgtggt
     3721 ggtggcaagc aggttgtttg tagcaaaggc gactacgagg aagaaaagat taaataagag
     3781 gaggggcccg aaagtaaatt tcaggctcct tttggttaga acttgacaaa atacatcatt
     3841 ttatgcgaaa ctagaagaag attattttaa ctaggagaag ttatgcgtct tatctatctg
     3901 ataattggtt ttttatcact ggccttggct attgttgggg ttgttttacc cttgttgccg
     3961 acaacgcctt tccttttgtt gtctattgct tgtttctcca gaagttccaa gcgtttcgaa
     4021 gactggcttt atcataccaa gctctatcaa acatatgtag ctgattttcg cgagactaag
     4081 tctattacgc gtgaacgaaa gaaaaaaatc atcgtctcta tctacgtctt gatgggaatt
     4141 tctatttatt ttgcacctct tttaccagtc aaaatcggtc tgggtgcttt gaccatcttt
     4201 atcacttatt atcttttcaa ggtcattcca gacaaagaat agttaaaaca gtagttattt
     4261 gccttgataa aattgaaagc atattcataa taatatgata taataaattt gaagtaatat
     4321 tcaaggagaa tcaaatgatt tacgaatttt gtgctgaaaa tgtgacttta cttgaaaaag
     4381 cgatgcaggc tggagctcgt cggattgaac tctgtgataa tctagcagtt ggtgggacaa
     4441 cacccagcta tggagtgact aaggcagcgg ttgaactggc agctaactac gatacaacca
     4501 tcatgaccat gattcggcca cgtggtggtg actttgtcta taatgaccta gaaattgcta
     4561 tcatgctaga agacattcgt ttgactgctc aggctggaag tcaaggggtt gtatttggaa
     4621 ctttaactgc tgataaaaag ttggataagc ctaatctgga aaagttaatt gctgcatcaa
     4681 aaggaatgga aattgtcttt cacatggcct ttgatgaact aagtgatgaa gatcaaccgg
     4741 aagctattga ctggctcagt caagccggtg tcactcgtat cctaactcgt gctggtgtgt
     4801 ctggcgactc cttagaaaaa cgttttgttc actatcacag aattttggag tacgctaaag
     4861 gtaaaattga aattctacca ggtgggggga ttgaccttga aaaccgtcaa acctttatcg
     4921 accaggtggg ggtaacacaa ttgcatggta ctaaggttgt tttttaaaaa atagaaagga
     4981 actgctagct ttgggtagca gttttcactt atgtttgaaa tttttaaatc ctatcaattt
     5041 aatcaagaaa aggctcatga ttatggtttt atagaaaata gcgaagtctg gacatatagt
     5101 tgccagattt tgcaaggtga ctttgtcatg actgtgtcca tcactgctga taatgtgaac
     5161 tttcaagtct ttgaccaaga gactggtgac ctctatcctc acgttcatat ggaaagcatg
     5221 aggggaagtt ttgtcggaaa tgtccgtgag gcttgtctgg agattcttta ccagattcgg
     5281 aaggcttgtt ttgatgtgca agattttatc tgtcatcaga ctaagcgtat catgactcaa
     5341 gttcaggaaa agtatggaaa ccagttggag tatctgtggg aaaaatcgcc tgatacagct
     5401 gtattgcgcc atgaaggcaa tcaaaagtgg tatgccgtct tgatgaaaat ctcttggaat
     5461 aagctggaaa agggaagaga aggacaagtg gaagcagtca acctcaagca tgaccaagta
     5521 gctaatttgc tttcacaaaa ggggatttat ccagccttcc atatgagcaa gcgctactgg
     5581 attagtgtgt cccttgatga tactttatca gatgaagaag tactggaatt gatagaaaaa
     5641 agttggaact taacctctaa aaaatgaaat attttaataa ttttcatgaa ctttcaatta
     5701 gctaaatatt ctttactgaa gaaattttca gaaaatatag gatttaccac accagaggaa
     5761 tatggtgcca tcttcaaata cctgattgag aatgtcaaga cggatcgtca gatcatctat
     5821 tcgcctcact gtcatgatga cctcggaata gcagtggcaa atagccttgc tgctgtcaag
     5881 aatggtgcag gacgtgttga agagactatc aatggtatta gggagcgagc tggaaatgct
     5941 gctttggaag aaattgcagt ggctctcaat attcgccaag attactacca agtagaaacc
     6001 agtattgtcc taactgagac catcaatacg tcagaaatgg tttctcgctt ctctggtatt
     6061 ccagttccta aaaacaaagc cgtcgttggt ggcaatacct tctcccacga atctggtatt
     6121 caccaagatg gagtccttaa aaatcctctc acttatgaga tcatcacacc tgaattggtt
     6181 ggtgttaaga ttccgcttgg aaaattatct ggtcgccatg cttttgttga gaaactgaga
     6241 gaattggccc tagattttac agaagaggat atcaaaccac tctttgctaa gttcaaggca
     6301 ctggtcgata agaagcaaga aatcacagat gcagatattc gagctttggt agctggaacc
     6361 atggttgaaa atccagaagg cttccacttt gatgatttac aacttcaaac tcatgcagat
     6421 aatgacattg aagcgctcgt tagcctagcc aatatggatg gtgagaaagt cgaatttaat
     6481 gcgacagggc aaggttccgt tgaagcaatc tttaatgcta tcgataagtt ctttaaccaa
     6541 tctgttcgtt tggtgtccta cactatcgat gcggtaacag atggaatcga tacccaggaa
     6601 cgggttttgg tcactgttga aaacagagat acagaaacca tcttgaatgc agcagggctt
     6661 gattttgatg tgttgaaggc ttctgctatt gcctatataa acgctaatac ctttgttcaa
     6721 aaagagaatg caggtgagat gggacgcagt gtttcttacc acgatatgcc tagtgtgtaa
     6781 aggagaaggc tatggcaaag aaaatagtag ctctagcagg agacggaatt ggcccagaaa
     6841 tcatggaggc tggtttagaa gttctggagg ctctagctga aaaaacaggt tttgactatg
     6901 agatcgttcg gaggtgcaga tattgatgca gcaggatctc ccttacctga tgaaaccctt
     6961 aaggcaagta gggaagcaga tgctatccta ctagtagcta tcggtagtcc tcagtatgat
     7021 ggagtagcgg ttcgccctga acaaggcctg atggctctcc gtaaggaact caatctttac
     7081 gctaatattc gtcctgtaaa aatctttgac agtctcaagt atttgtcacc actcaaaccg
     7141 gaacgaattt ctggtgtaga ctttgtcgtg gtgcgtgaat tgacaggcga gatttacttt
     7201 ggagatcata ttcttgaaga gcgcaaagcg cgtgatatca acgactatag ctatgaggaa
     7261 gtggagcgga ttattcgcaa agcctttgaa attgcaagaa atcgcagaaa aatcgttact
     7321 agtatcgata agcaaaatgt tctagcgacc tcaaaactct ggcggaaagt agctgaggaa
     7381 gtcgcacagg atttcccaga tgtaaccttg gaacaccagc tggtagactc agctgctatg
     7441 cttatgatta ccaatcctgc taagtttgat gttattgtaa cggagaatct ttttggagat
     7501 attttatctg atgaatcaag cgtcttatct ggtacacttg aggttatgcc atcagccagt
     7561 cattctgaaa atggaccaag tctctatgaa cctattcacg gttcagcacc tgatattgca
     7621 ggtcaaggaa ttgccaatcc tatttccatg attttattag ttgtcatgat gttgagagat
     7681 agtttcggac gttatgagga tacagagcgt atcaaacgtg ctgttgagac aagtctggcg
     7741 gcaggaattt taacgagaga tataggaggt caggcttcaa caaaggaaat gatggaagct
     7801 attattgcaa ggttatgaag ttagacgaaa aaattactct agtccttttg atttggaatg
     7861 tcatcatttt cttgatttat ggcattgaca aattcaaggc aagaagaaga gcttggcgca
     7921 tcccagagaa aatcttactc attttagccc ttacttgtgg tggttttggg gcctggttag
     7981 ctggaatcac ttttcaccac aagactcgaa aatggtactt taaaacagtt tggtttcttg
     8041 ggatggtgac cacactagta gccttatatt ttatttggag gtaatggatg gtagggtctt
     8101 cgagggaaca cgctgcttgg gctctagcgg actatggttt taaggtcgtg attgcaggat
     8161 ctttcggtga cattcattac aataatgaac tcaataatgg catgttgcca atcgttcagc
     8221 ctagagaggt tagagagaaa ctagcccagc taaaaccaac cgaccaggta actgtggact
     8281 tggaacaaca aaaaatcatc tcaccagttg aagaattcac cttcgagata gatagcaagt
     8341 ggaaacataa actcctaaat agtttggatg atatcggtat taccttgcag tatgaagagt
     8401 tgattgctgc ttatgaaaaa caacgaccag cctactggca ggattagaaa aaatagaaaa
     8461 ggaaatatag taaactgaaa taagatgtaa acaaatgaat tggagcttaa catccatttc
     8521 cagcaatttt ttagaaacta cagtggacta ttctggattc aacacattat aaaattatga
     8581 caaaacacat tcacaagaag gctacgacat tttaaaaggt gagggcggat gtatcgtttg
     8641 ccctactaaa gttggttaca ttatcatgac cagcaacaag gcaggacttg agcgtaagtt
     8701 cgcagccaaa gaacgtaagc gtaacaaacc aggtgttgtt ctctgcggta gcatggatga
     8761 acttcgcgct ttagcgcaac tcaacccaga aattgaagca ttctactaaa aacattggat
     8821 gaagatattc ttcttggttg tatccttcct tggaaaccag aagcctttga aaaactcaaa
     8881 gcatacgggg atggccgtga agaacttatg actgatgtac gtggtactag ctgttttgtt
     8941 atcaagtttg gaaaagcagg tgaacaattg gctgccaagc tttgggaaga aggtaaaatg
     9001 gtctacgcct catatgcttc aatgacaaaa cgattgaaac tcgctatgag agcaaggtgt
     9061 aatggtgtct atggtcgata aggacggcaa actcatccca gaacaaggag gagcacgttc
     9121 aacttcacca gcaccagttg tgatccgtaa agggcttgac attgataaaa tcatgatgca
     9181 cctgtcagat acttttaact catgggacta ctgtcaggtt gagtattatt aagataaaaa
     9241 aagaagtcta gtgttatgag atattaaagc tcctaacact ggactttttg tttagaattt
     9301 cttt
//
LOCUS       DAVLXF010000076         8734 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLXF010000076 DAVLXF010000000
VERSION     DAVLXF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.076"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(61..951)
                     /locus_tag="VYO12_001686"
     CDS             complement(61..951)
                     /locus_tag="VYO12_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1588249.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            1084..1620
                     /locus_tag="VYO12_001687"
     CDS             1084..1620
                     /locus_tag="VYO12_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1588250.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(1786..2592)
                     /locus_tag="VYO12_001688"
     CDS             complement(1786..2592)
                     /locus_tag="VYO12_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1588251.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(2937..4457)
                     /locus_tag="VYO12_001689"
     CDS             complement(2937..4457)
                     /locus_tag="VYO12_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1588252.1"
                     /translation="MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLNGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITENQMSQLNYQVGVEGKSAEQVAKEFLQEQGLLKK"
     gene            complement(4450..5178)
                     /locus_tag="VYO12_001690"
     CDS             complement(4450..5178)
                     /locus_tag="VYO12_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588253.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKILLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVS
                     NPETILKAPATDFVADLFGGSIHD"
     gene            complement(5193..5957)
                     /locus_tag="VYO12_001691"
     CDS             complement(5193..5957)
                     /locus_tag="VYO12_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1588254.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(5961..6404)
                     /locus_tag="VYO12_001692"
     CDS             complement(5961..6404)
                     /locus_tag="VYO12_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1588255.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(7086..7382)
                     /locus_tag="VYO12_001693"
     CDS             complement(7086..7382)
                     /locus_tag="VYO12_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1588256.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(7404..8468)
                     /gene="pepA"
                     /locus_tag="VYO12_001694"
     CDS             complement(7404..8468)
                     /gene="pepA"
                     /locus_tag="VYO12_001694"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1588257.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPYVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         2612 a         2071 c         1489 g         2562 t
ORIGIN      
        1 aataataaag aaattactct attccaaata agatagtacc caagtatttt tcactttttt
       61 ctaatgttga tgctcataac tccgttccaa gtcacacacc tttcgcttat gggtttggtg
      121 agtttctagg tcagcatcaa tttcaatggt aatattttga aaaccacaat cttttaggaa
      181 aattcgaata gactctttac aagtttccat atgttccatt tcttttagac aaacatggac
      241 aatggcattt ttttccaaag catccatagt ccagagatta agctgattaa ggctggccac
      301 attgtccaat cgctccaggc cactctttac ttgcttgata tcaagacctt ctggcacagc
      361 atccaagaaa atcttgagtg tagaccaaaa acgtggaagg gcttttgaaa gaataaagaa
      421 agaaatgaca agggacaaaa gaggatctag gatataccag tccgtaaatc gaagaacaat
      481 cgccatcagg ataacagcta cccaccctag cgtatcttcc agaaaatgca gactcagaat
      541 agactcattc tttgtctttc ccttaccaac caccagactc gctaacagat tgatagtaat
      601 cgcaataatt cctaaccaga gaatcccctc atcattgact ggttgcggat gcaaaatctt
      661 cgtgacattt tccaaaatga ctagaacaga gcccgttacg agaatcacag ctgttaccaa
      721 ggctcctagc aggctaaacc gcttatagcc caaggtgtac tgattgtctt cttcacgatt
      781 ggagattgtt tctagaaaag ctgatattcc aattgcaatc gcatctccca agtcatgcac
      841 agagtcagca agaacagcgc tagaaccaaa tactccacct gcaataaact caacaatggc
      901 ataagtcaaa tttaagaaaa aagccaccca aacagcatat tttgccttca tatttctcat
      961 tcctttgtta taatagattt atgaacacct tgttcattat cattatccac taaaactcaa
     1021 agaaaggata gaagcaaaac gtcagcttta ttcagttctg aacaatttgc ctcaagtgtc
     1081 catatgacta acattgaccg ccgtatcagc aaaaccaaaa aagccatcta tcaagctttt
     1141 atacaacttt tgaatgctaa gggctacgag gccactactg ttcaggatat cattgatctc
     1201 gcagatgtgg gacgatccac cttttactgt cactatgaga gtaaggagct tcttctggac
     1261 cagctctgcc gctacctctt ccatcatctt tttgaaagag agcaagccat ttcaaccgag
     1321 gactacctcg cccacctctt tctgcatttt cataaaaacc aagaccatat caccagtctg
     1381 ctattttcca aaaatgacta cttcctccgt caactccata aagagctaga acaccatgtc
     1441 tattccgtgc tagctgataa tttaaaagaa gcccacccta atctgcctac ttcttacctc
     1501 caacacttgg tcatgtccaa ctttatcgag acattgacct ggtggctcaa aaaaggtcaa
     1561 gatttcacag accaggaagt tgtccaattt tatctagacc ttctcattcc taaaaattga
     1621 atatagagta aagctcagtt gtcttatttc tagattactg agttttttat cttttcaaca
     1681 acaaaagagg acccgccgat cctctttttc atactataaa tccttgatta tcaactatat
     1741 ctgttttaat cgaaatctca aaacagcact ttcaaacatc ttttcctagt taagtaaatc
     1801 agtattctgc ttagctgcct tgctccattg ataccaacca actagactgt taatgagata
     1861 aattagatat ttcccttgaa tttgcaggct ttctccccac cagagataga ttgaaaagac
     1921 attggtagcc gcccagaata tccactgttc acggtaaaca gctgtcatga ggatttgccc
     1981 taccccattg gttgcatctg tgattgaatc acgataggga cgattggcac caatagactg
     2041 ataaatgaag ccaaaggcca accaccaaag cacactaatg gaaagatact ttgtccagcc
     2101 cttgccgtcc agtttacgcg cgacaaactc ctgcttttcc ttcttaaact gtgcctgata
     2161 aatccaaact agaagtccaa ttggctgcat gactgtgaag taaagtgtcg tcagcacctc
     2221 accataaaag cctttctgta gggccaaaat aaggtaaata acagagttaa tcaagccaaa
     2281 aagataatta cttgctcgac cttccgatac aaagattaca cagataatcc ctgtcaagct
     2341 acaaatcatc ccaatccagt caacaatacg atgttcgtaa accaactcca gccagagagg
     2401 aaaacttcct aaaaccagca aataaatcca ctgggcaaaa ctacgatggg caaagaggtc
     2461 atcccagata gccttcatag ttcctgaaaa tcctaaatca gccatagccg caaccatacg
     2521 acggtaacca cctgacattt cacctagggt tgttttgata ttttcaattt tcttttgcaa
     2581 ataagtatgc atcatttctc cttttgtttt taaagagccg tgtctggata gactttcgga
     2641 cgcaacgctc tattagataa tgaactgcct atacacaaga tttctaacct tagtcgacat
     2701 gagctgaaac ctcttatttg ttaagtagtt cacaaaatat tatacaccta ttttatgaat
     2761 agtcaactgt ctttacagta aaattttaga aaatcatgaa aattttctct ttctttccat
     2821 tttaagtgac attcagtcat tctcacatca aaaaagccca gacgaaattg tctgagcatt
     2881 cttttatcta gtcgtttaag gaagttgagt tcagtatgtt taaagtctct gtcccatcat
     2941 ttcttcaaca aaccttgttc ttggagaaac tccttggcta cttgctctgc tgacttgcct
     3001 tcaacaccga cttggtagtt gagctggctc atctggtttt ctgtaatctt accagccaat
     3061 gtattaagaa ctctttccaa ctctggatgt ttcttgagaa gagcttcttt catgagtgga
     3121 gccccttgat aaggtgggaa gagttgcttg tcatcttcca agacctgtaa atcataacgc
     3181 tccaattccg catcagtcga ataggcatcc gtgatttgaa tatcccctga ctgaattgcc
     3241 tgataacgaa gagctggctg catagtcgcc acattgagat tgagaccata cattgattgc
     3301 aagcccttat ttccatcttc acggtcgtta aactcaagcg taaagcctgc cttcagctgc
     3361 ccttccactt ttttcaaatc cgaaatggtc ttcaagccat attcttgagc aatctttttc
     3421 ggaacagcta cagcataggt attttgataa gacataggtt tgagataggc tagatgatcc
     3481 tgcttggcaa tgccatcacg cgccacttga taaacctgct ctggctcatg actcaccttg
     3541 ggtgatggtt gaagcaaact ttcagtcacc gtaccggtaa attcaggata aatgtcaata
     3601 tcaccttttt tcagagcttc ataaaggaaa cttgtcgtcc caaaattcgg tttaacagtc
     3661 gcagtcatgc tggtattttc ttcaatcagc aatttataca tattagccaa aatttctggt
     3721 tctgggccta ttttcccagc aataatcaag ttttccttct ctttttgagc caaaagggct
     3781 ggactataag acagacccag taataaagcc accaaggcaa aaccagagaa aatcgtccgc
     3841 aatttcgctt tttccattac ttttagtagg aagttaaagg caatagctag cactgcagaa
     3901 gaaagtgccc caatcaaaat cagactggca ttattacggt caattcccaa aaggataaag
     3961 gaacctagtc cccctgcccc aatcaaggtc gccaaggtag ccgtaccgat aatcaagacc
     4021 gctgccgtcc gaattccaga catgataaca ggcatggcaa gtggaatttc aaacttcttg
     4081 agacgctccc atctggtcat cccaaaggca atcccagcct cttgcagact cggatcaatt
     4141 ccattcagcc cagtaatagt attttgtaaa atcggaaaaa tcgcataaat cactagagct
     4201 gtcaatgccg gtaaagtccc aattcccatc aaagggataa agagccccaa caaggccaga
     4261 gacgggatgg tctggaaaat acctgcaatc tgcaagaccc agtcggccag cttctcatga
     4321 tagcgaagat aaacagccaa gggaatcgca agcaaaatag ctagtaacaa ggtcaaaagt
     4381 gacaactgca aatgttgaga tagggctgtc aaccaatcac caaaacgatc ctgaaaagtt
     4441 gcaattaaat tagtcatgaa tactacctcc aaacaagtct gcaacaaagt ctgttgcagg
     4501 agcttttaaa atagtctcag gattcgacac ctgacgaatc tctccatcct gcaagacagc
     4561 aatacgatct gccaacttta aggcttcatc cgtatcatga gtcacaaaaa tcgttgtcat
     4621 cccaaactct ttatgtaatt cttttgtcag aacctgcaac tgttttctcg aaatagcatc
     4681 caaggctgaa aagggttcat ccatgaggag aatcttgggc tgaccaatca tagctcggac
     4741 aataccgacc cgttgctgtt ctccaccaga tagctcactt ggaagtcgat gcccatactc
     4801 agctactggt aaaccaacct tagccaaaag ctcttctgtt ttttgagcaa tttcttcctt
     4861 agtccaaccc ttcatttcag gaatgagagc aatattttcc tcaactgtta gattgggaaa
     4921 gagagcaata gcctgtaaaa catatccagt agaaagacga agttcacgct catcatagtc
     4981 tttgatgcgc ttcccatcca tataaatatt tccatcagtt ggttccaaaa gacggttaat
     5041 catcttgagc atggtcgtct tacctgaccc agaaggccct actaaaacca taaattcccc
     5101 atcctcaatc tgtaagttga catctctcaa gacatccttt tctgtgtagc gcagtgctac
     5161 atttttgtat tcaatcattc tttgtcctca atttaaaact tccctcgatt agtcaagtct
     5221 tctaccttag gcatgacttc cttattatcc caatgctcca caattttccc gttctctaaa
     5281 cggaagatat cgtactgggc ataagcaacg ccatcaatct gagtctgacc atagctaacc
     5341 acatagtttc cttgtcctaa gagttggaaa acaaagtcaa aagtgacact atattcagcc
     5401 acatagtttt tataagcagc acttccttgt ccaatctcat gattatgctg aatcaaatcg
     5461 tctgccacat aatcactcca ctgctctagc tccccatttt ggaaaatttc tgtcaagaaa
     5521 cggcgaacca gctttttatt ttctgctttc ttatccaaat ccttgatttc aaaatctcca
     5581 aaaatttgat ctagttggtc attttcaggt gttcgatagt agtcaatgac atcccaatgc
     5641 tcaacaatac gaccattctc atccgcacga aaagtatccg tcgtcaccca ttgagcttct
     5701 ccaccattca gatattgatg aacatgaaca aagaccagat tgccatcctc aatggtgcgg
     5761 acaatcttaa tctgacgctc tggatgacgc tcaaagaaat ctgcaaagaa ggctgcaaat
     5821 ccttctttcc cgtcaggaac acctgtcgaa tgttggatat aggtatcccc tacagactgg
     5881 gcttgagcct cagcaactcg tccgtcttga atggcatgga tgtataggtt gtgagcattt
     5941 ttcacttgtt gtgacatatt ctaaacctca tttcccttct ctttcaaatt cgccaaaatt
     6001 ctttcttgaa aaccttcaaa ttggtgaatt tcttcctctg aaaatccttt gtaaaagata
     6061 gtatccaatt tctgactgac acgatgcccc acttctttct gggacttgcc taactccgtt
     6121 aaaactaaat acttcttacg cttgtctttt ccacacggac taacaattac aagcttttgt
     6181 tcctctagct tttttatcat agtcgtcagc gtattattcg caagcccagt cgcaagcgcg
     6241 atatctgtcg cagttgcgca gccagtttca ctattccata aaaccgctaa aatcttgccc
     6301 tgttcacccc tataaagagc ctcaggatct tgactcagta acttttgaaa aatccgccca
     6361 ttcaacaaac gaatatgatg ggctagcaaa tgaccatctt tcataacacc tccaatttat
     6421 ttcgatatcg aaatgaataa aacaattgta acactcatcg ttctaactgt caactatttc
     6481 gatttagaaa taatttttga taattatcca caccaccata ctccggctca actaactttt
     6541 aacgagagtt tctaaactcc ttcgtcctcc agtctacaaa agccttccat tcgtactatc
     6601 ctatatttta tgaggggaca catttttcct atcagaccat ttattttaaa gatagaagta
     6661 aatcataatt gcttccatct gttcttttat agtatattga agttagacta gagcactgta
     6721 tcttctaaaa cattgataga aagcgatttg aatttcccaa tcaatttgtt cgtatttata
     6781 gcatttcgaa actggaatag gacaccatga ctgctaaaag atttctataa attcatttga
     6841 ttttcctaat caatttgttc atatcttatt tcattccgct ataatttcac cttaccctat
     6901 ctttttcgta gcacccttca aacagcctat cccctaccgt ttgacgattc ctcacttcgc
     6961 tccacttcca ttacagaagt ttcttcacta ctatgggctc ggctgacttc tcatgattcc
     7021 ttgttactac tatttgaacg ctcacgagat agatcttaca aaaaatgctt tgatccacaa
     7081 tggaatcaaa gcattttaaa gagttcctca tacataagcg cagaagtcgc agttcctctg
     7141 tacttggctt cttctctttt gataaagcga gccaagttga gcaactcagg tgctggatgt
     7201 ttgggattta ggagcaattc acgattgacc aggcctgaga gacgaactgc ctgcaattgt
     7261 tcatttgtag taggcagttt tttagcagtc tctaggagag cagcaactaa atcttcactc
     7321 aaatcatgtc gagcatgatt gtaaagatct tttataaggc tttctaggtt tggttctgcc
     7381 atccctacca cctcccttat ggtttaataa tgtttaatca aatcaaccgt tgaacgatcc
     7441 aatttcttca ccaaggcttg taagaaagct tgcgcttcta ggaagtcatc cattgcatag
     7501 agggtttggt gagaatggat ataacgagcg cagacaccga tagttgttga tgggacacca
     7561 ccatttttca gatgagctgc acctgcatct gttccgcctt taccacagta gtattggtac
     7621 ttgataccag cttcttcagc cgttgtcaaa agaaaatcct tcatccctgg gagaagcaag
     7681 tgacctggat catagaaacg aatcaaggtt ccatctccaa ttttgccttg accaccgtag
     7741 acatcacctg ctggtgagca atcaactgcg aggaagactt ctgggtcaaa cttggttgta
     7801 gaggtatgag cgccacgcag accaacttct tcttggacgt tagaacccag atagagttca
     7861 ttgccgagtt tttgacctga taaagcttca gctagctcgc ttaccatgag gacaccgtag
     7921 cggttatccc aagcttttga gatgatattt ttttcattgg ctgtcaaaat tgcagaacta
     7981 tctggtacga tggtatcacc aggacggatg ccaaaacttt ctgcctcagc cttgtccgca
     8041 aaaccaccat caaaaacgat atcggcaatg gctggcatgg ttggtccccc ctttccacga
     8101 gtcaaatgcg gaggaacaga acctgaaatc acaggaattt catgaccatc acgagtcaag
     8161 agtttgaaac gttggctgct aaccaccatg gggttccagc caccgatttc tacgacacgg
     8221 aaggtaccat ctggcttgat ttcgctgacc ataaaaccaa cttcgtccat atgagaagcg
     8281 accaagacgc gcggtgcatc cacagcttct gaatgtttga taccaaaaat accacccaag
     8341 ccatctgtca ccacttcatc cacatacggt gtcaactttt cacgaagata agcacggaca
     8401 ggcgcttcat gacctgagac tgcagcaagt tctgttactt ctttaatttt tgaaaataat
     8461 gttgtcattt cagttccttc tttctttcat ccattttacc actttttata ggagaaggat
     8521 agtgggaagg tggatttcta agttagtatc ttagtcctgc tctatcttag aaaaggatag
     8581 tattctcttg catgtagtgc aaaatctagt aaacattcca aaattaactc gaatatttat
     8641 ttccaaacaa aaaaacaata caccatcaaa gttgtttaga tttttcatga aatttacaga
     8701 aaatagttga cttccctttc ttctttcttt aaat
//
LOCUS       DAVLXF010000077         8718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLXF010000077 DAVLXF010000000
VERSION     DAVLXF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.077"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            44..199
                     /locus_tag="VYO12_001695"
                     /pseudo
     CDS             44..199
                     /locus_tag="VYO12_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            399..3674
                     /gene="rexB"
                     /locus_tag="VYO12_001696"
     CDS             399..3674
                     /gene="rexB"
                     /locus_tag="VYO12_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1588258.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTDYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYCFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3671..7321
                     /gene="addA"
                     /locus_tag="VYO12_001697"
     CDS             3671..7321
                     /gene="addA"
                     /locus_tag="VYO12_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1588259.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAANELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWELAKTFQTFMSHFVEVYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYIFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLTSLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7491..8669
                     /locus_tag="VYO12_001698"
     CDS             7491..8669
                     /locus_tag="VYO12_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588260.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERDIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2688 a         1839 c         1862 g         2329 t
ORIGIN      
        1 ctatttttgg ttcattttac tatagttcaa tcattctatc aagaatattg aatatataaa
       61 ggcacgtaat attaaaactt cttctgatat cgaattagag tccgatgttg atggcgataa
      121 atcagacaat ctacccgtcg acattaagac actgggacaa cacattgaag tgtattccgt
      181 acctaagaaa caatcataaa actcaaagcc ttgttaccga ggctttttta ttatatgatt
      241 ttcagtggcc agacttattc tatctaatac aagtaaagct aagtatttaa gatttagcag
      301 atgatgaaga aaagaaagga tggcctaaat attttaacag atttacgagg ctaatcatgg
      361 tataatatag ggtgatgaaa aattccaacg aggctgagat gaaattactt tatactgata
      421 ttcgaacttc tttgacagaa attctaacaa gagaggcaga agagctagtt gcagctggca
      481 agcgggtctt ctacattgcc cccaactctc tttcttttga aaaggaacgc gccgtgctgg
      541 aatgcttgtc ccagcaggct tctttttcga ttaccgtcac gcgctttgct caaatggctc
      601 gttatctggt cttgaatgat ttaccagtta aaactactct tgatgatatc ggtcttgggt
      661 tggcctttta caaatgcctt gccgaactcg atcccaagga cttgcgtgtt tatggagcta
      721 ttaagcagga tcctcaattg atccagcagt taattgagct ttaccatgag atgaccaaat
      781 ctcagatgaa ttttttggac ttggagaatt taacagatga ggataagagg gcggatttac
      841 tcttgatttt tgagaaagta acagactatc ttaatcaagg tcagttagcc cagggaagtc
      901 agttgtccca tttgattgag gctattgaga atgacaaggt aagtagtgat tttaatcaaa
      961 tcgccttggt cattgacggc tttactcgtt tttctgctga ggaagagcgg gttgtggact
     1021 tacttcacgg caaaggtgtt gagattgtta tcggggctta tgctagtaag aaagcctata
     1081 ccagtccttt tagcgagggc aatctctacc aagccagcgt aaaatttctc catcatctgg
     1141 cttctaaata ccaaacgccc gctcaggact gttctcaaac tcatgagaag atggatagtt
     1201 ttgacaaggc ctctcgtttg ttggagtctt cttatgactt ttcagaactc gctttggatg
     1261 tcgatgagaa agaccgtgaa aatttacaaa tctggtcttg tttgacgcaa aaggaggagt
     1321 tggagctagt agcccgtagc attcgtcaga aattacatga gaactcagac ctgagctaca
     1381 agcattttcg tattctcttg ggggatgtag cttcttacca gttatctctc aaaaccattt
     1441 ttgaccagta tcagattcct ttttatcttg gtagaagcga agccatggct catcatccct
     1501 tgactcagtt tgtcgagtct attttagctt taaaacgtta ctgttttcgt caggaggatt
     1561 tgattaatct tcttagaact gacttgtata ctgacctcag tcagtctgat attgatgctt
     1621 ttgagcaata tatccgctat cttggtatca atggcttgcc agcctttcag caaatcttca
     1681 ccaaatccca ccatggaaaa tttaatcttg agcgtttgaa tgtcctccgc ctgagaattt
     1741 tagcacctct tgaaaccctc tttgccagcc gaaaacaaaa ggctgaaaac ctcctacaaa
     1801 aatggagtgt ctttctaaaa gaaggagctg tgaccaagca gttacaagat ttgacaacca
     1861 ctttggaagc tgtagaacag gaaagacaag ccgaagtttg gaaggctttc tgccatgttt
     1921 tagaacaatt tgcgactgtt tttgctggtt cacaggttag tctggaagac ttcctagcct
     1981 tgctccattc tggaatgagt ttgtcccaat accgtaccat tccagcaaca gtggacactg
     2041 ttctggtgca gagttacgat ttgattgcac cattgactgc tgactttgtc tatgctattg
     2101 gactaactca ggacaattta ccaaaaattt ctcaaaacac cagtcttctg acagatgaag
     2161 aaaggcaaaa tctaaaccaa gcgaccgaag aaggcgttca attactgatt gccagcagtg
     2221 aaaatctcaa gaaaaatcgc tacactatgc tttccttagt caattctgct cgtaagcagt
     2281 tgttcttgtc ggctccaagc ctttttaacg aaagtgaaag taaggaatct gcctatcttc
     2341 aagagttgat ccattttgga tttaggcgga gagagaagag gatgaatcac aaaggactgt
     2401 ctaaggagga tatggggtcc tatcacagtc ttttgtctag tctggttgcc tatcaccagc
     2461 agggtgagat gagcgatact gagcaagatt tgacttttgt caaggttctg tcgcgtgtca
     2521 taggtaaaaa actagatcag caaggtctgg aaaatccagc catcccaatc agtccaagca
     2581 gcaagacctt agccaaggac accttgcaag ctctctatcc tgccaaacag gagttttacc
     2641 tgtctacgtc gggtttgaca gagttttatc gcaatgaata cagttatttc ctacgctacg
     2701 ttttaggctt gcaggaggaa ttacgtttgc gtcctgatgc ccgtagtcac gggaatttct
     2761 tgcatcgtat ctttgaacgc gccttgcagt tgcctaatga agattccttt gaccaacgtc
     2821 tagaacaagc tattcaagaa accagtcaag aacgcgaatt tgaagctatt tatcaagaaa
     2881 gtttggaagc ccagtttacc aaggaagttt tgcttgatgt tgcacggaca actggacata
     2941 ttctccgaca caatccagcc atcgaaacaa tcaaagaaga agcaaatttt ggtggaaaag
     3001 accaagcctt tattcaatta gacaatggac gcagtgtctt tgtacgaggc aaggtggacc
     3061 ggattgaccg tttgaaagct aatggagcga taggagtagt agactacaaa tccagtctga
     3121 ctcagttcca gtttcctcat ttctttaatg ggctcaattc tcagttacca acctatcttg
     3181 ctgccctaaa aagagaaggg gagcagaact ttttcggcgc catgtacttg gaaatggctg
     3241 aacctgtcca atctctgatg gcggtaaaaa gtctggcagg agcagtggta gaagccagca
     3301 aatctatgaa ataccaaggg ctcttcttgg aaaaagaaag cagttattta ggcgaatttt
     3361 ataacaaaaa caaggcgaat caactgacag atgaggaatt tcagctccta ctggactaca
     3421 atgcctatct ttacaagaaa gctgctgaga agattttagc aggccggttc gccatcaatc
     3481 cttatactga aaatggcaga agcattgccc catacgtcca gcaacatcag gctattacag
     3541 gctttgaagc caattaccat ctgggccaag cccgtttcct agaaaagttg gacctagctg
     3601 atggcaagcg tctggtcgga gaaaaactca agcaagcttg gcttgaaaaa ataagagagg
     3661 agttgaatcg atgaagctta ttcccttttt aagtgaggag gagattcaaa aactgcaaga
     3721 agcagaagca aattcgagca aggaacagaa gaaaactgcc gagcaaatcg aagctatcta
     3781 cacttctggc cagaatatcc tggtctcagc atcggctggt tctggaaaga cctttgtcat
     3841 ggcagagcgc attctggacc aattggcgcg tggtgtcgaa atttctcaac tctttatctc
     3901 aacctttacc gtcaaggctg caaatgaact taaagaacgt ttagagaaaa aaatcagcaa
     3961 gaaaatccaa gaaacagatg atgtcgacct caaacaacac ttgggacgcc agttggcaga
     4021 cctaccaaac gctgccatcg gaaccatgga ctctttcaca caaaaattcc ttggcaaaca
     4081 tggttatctg cttgatattg cacctaattt ccgtatttta caaaaccaaa gcgagcaact
     4141 tcttctcgaa aacgaagtct ttcatgaggt ctttgaagcg cattaccaag gtaaacagaa
     4201 agagaccttt agtcatttgc tgaaaaactt tgctgggcgt ggcaaggacg aacggggtct
     4261 gcgccagcag gtctataaaa tctatgactt cctccaatcc accagtaatc ctcaaaagtg
     4321 gctgagtgaa tctttcctca aaggctttga gaaagctgat tttaccagtg aaaaagaaaa
     4381 actgactgag caaatcaaac aagccctttg ggatttggaa agctttttcc gttaccatct
     4441 ggataacgat gccaaggagt ttgcaaaggc tgcctattta gaaaatgttc agttaattct
     4501 ggatgaaatt ggctccctaa atcaggagtc cgatagtcag gcttatcagg cagtgcttgc
     4561 gcgtgttgtc gccatctcta aggagaaaaa cggtcgagct ctgactaatg ccagtcgtaa
     4621 ggctgatttg aagcccctgg ctgatgccta caacgaagag agaaagaccc agtttgctaa
     4681 actaggacaa ttatcagacc agataaccat tctcgactat caagaacgtt atcatcaaga
     4741 cacatgggaa ctagctaaaa ccttccaaac ctttatgagt cattttgtgg aggtttatcg
     4801 tcagcgcaaa cgccaggaaa atgcctttga attcgctgat atcagccatt acaccattga
     4861 gattttagag aatttcccac aagttcgtga gtcttatcag gagcgcttcc atgaagtcat
     4921 ggtcgatgag tatcaggata ccaaccatat tcaagaacgg atgctggaat tgttgtctaa
     4981 tggccacaat cgctttatgg tgggagatat caagcaatcc atctatcgtt tccgtcaggc
     5041 agacccgcag attttcaatg agaaattcca acgctatgcg caaaatcccc aagaaggcaa
     5101 gctcattctc ctcaaggaaa atttccgtag tagttcagaa gtgctgtcag caaccaatga
     5161 tgtctttgaa cgtctcatgg accaagaggt cggcgaaatc aactatgata acaagcacca
     5221 gcttgttttt gccaatacca aactgactcc caatccagac aacaaggcag aatttctcct
     5281 ctacgacaag gacgatacag gtgaggaaga agagagtcaa acagaaacga aactaacagg
     5341 cgaaatgcgc ttagttatca aggagattct gaaacttcat caagaaaaag gtgttgcctt
     5401 taaggaaatt gcccttctga cctccagccg cagtcgtaat gaccagattc tcctcgccct
     5461 gtctgagtac ggaattcctg tcaaaactga cggagagcaa aacaattatc tccaatccct
     5521 agaagtgcaa gtcatgctag acactcttcg tgtcattcac aatcccctgc aagactacgc
     5581 cttggttgcc cttatgaagt ctccaatgtt tggttttgat gaggatgagc tagcacgttt
     5641 gtcccttcag aaagcagagg ataaagtcca cgaaaatctc tatgagaaac tggtcaatgc
     5701 acaaaaaatg gcaagtagtc aaaaaggctt gattcacaca gctctagctg aaaaactaaa
     5761 gcaattcatg gatatcctag cttcttggcg cttgtatgcc aaaacccatt ctctctatga
     5821 cttgatttgg aagatttaca acgaccgttt ttattatgac tatgttgggg ctttgccgaa
     5881 tggtcctgct aggcaggcca atctctatgc cctagcactg cgtgctgatc aatttgaaaa
     5941 gagcaatttc aaaggtttgt cgcgttttat tcgtatgatt gaccaagtct tagaagccca
     6001 gcacgatttg gcaagcgtag ccgtcgcacc gccaaaagat gcagtagagc tcatgaccat
     6061 ccacaagagt aaagggctgg agtttcctta catctttatc ctcaatatgg atcaagattt
     6121 caacaagcaa gactctatgt cagaagtcat tctcagtcgt cagaatggtc ttggtgtcaa
     6181 atatattgcc aagatggaga caggggcagt agaagaccac tatcctaaaa ccatcaaact
     6241 ctccattcct agtctgacct ataggcagaa cgaagaggaa ttacagctag caagctattc
     6301 tgagcagatg cgtttgctgt atgttgctat gacgcgggct gagaaaaagc tctatcttgt
     6361 cggcaagggt tctcgtgaaa agctggaatc caaggaatac ccagcagcca aaaatgggaa
     6421 actaaatagc aatactagac tgcaagcacg gaatttccaa gattggcttt gggctatcag
     6481 taaagtgttt actaaggaca agctcaactt tagttatcgt tttattggcg aagatcagtt
     6541 gaccagagaa gctatcggag agttggaaac caagagtcct ctccaagata gctcccaagc
     6601 agacaatcgt cagtcagata ccatcaaaga agctctggaa atgctgaagg aggtggaagt
     6661 ttataatact cttcaccgcg cagctattga acttcctagt gttcaaaccc caagtcaaat
     6721 caagaaattc tacgaaccag ttatggatat ggaaggtgtc gagattgctg gtcaaggtca
     6781 gtcagtaggc aagaaaatca gcttcgattt gccagatttt tcaaccaaag aaaaggtaac
     6841 tggagctgag attggtagtg ctactcacga actcatgcag agaattgacc tcagccagca
     6901 actaaccctt actagcctaa cagaaacact caaacaagtt caaactagcc aagctgtcag
     6961 agacaagatc aatcttgata aaattcttgc tttctttgac acagcactcg gtcaggaaat
     7021 tcttgctaat accgaccatc tctatcgcga gcaacctttc tccatgctca aacgagacca
     7081 aaagagtcag gaagactttg ttgtccgtgg tatccttgat ggctatctgc tttacgaaaa
     7141 caaaattgtt ctgttcgact acaagacaga ccgctatgat gaaccaagtc aactcgtaga
     7201 ccgctatcgt ggtcagttag ctctatacga agaggcttta tcacgagcct attcgattga
     7261 aaatattgaa aaatacttga ttttactcgg taaagacgag gttcaagttg taaaagtata
     7321 acttatactc ttcgaaaatc tcttcaaacc acgtcagctt cgccttgctg tatatatggt
     7381 tactgacttc gtcagttcta tccacaacct caaaacagtg ttttgagctg actgcggcta
     7441 gctttctagt ttgctctttg atttttattg agtattagaa aggagacctc atgacacttc
     7501 cagttagaaa atccctgcac gatgcagttt tacaggcttc gaaagcggat acttgggaac
     7561 aagctaccaa ggaatggaat gaagtttcct tgatttttaa cggtatcggc cgtagcaatt
     7621 gtgtctgtgg aaatgccatc aaatacgctt atgaactctt taacggtgtc acaggccaac
     7681 gcctctttcc aattggtagc gactgtgttc gtcattttca tcgattgtcc cttgaccagc
     7741 aattggaaga ggaagaaaaa ctgctcagaa aggttgaaaa tctaaccaga aaagctcaga
     7801 aaaaggaaaa aatcaaggtc aataaaagtg actttgacga gcgacttcta aaatggctct
     7861 gggaaaaagg tgttttcaaa cccaatcgtg gcaatcaatt tgcgcctgag agagactacc
     7921 agcttttcct agaagtcttt cagggaggaa gttggaccaa ggcggaacca aagaaaaagg
     7981 ctcggatgga agaagtcctt gaaaagtgta tcaaaccctt tttacttggg aaatcggatg
     8041 accaactcta ccttgtcaag ctaggcaagg agaaaattga ctacgaacaa gaactccgta
     8101 tccaggcaga gaaagaacgc aagaagaggg ataaaatcgc caagcaatat gcagacaatc
     8161 tggttctagc catgggaccc gcagagcgtg cttatcaaga ttactttggc tttacagaga
     8221 ccctgactca agaagaacgc aagtgggaaa aaattctttt tggtaaaaat agagcagaac
     8281 gggatatcaa ggccaaacaa taccaaaaag agctggaaaa agaccaacga attgccagtc
     8341 aggatccaat tgaaagaaaa cagaagcaga cttggctcct caattcctat tttcgtgagc
     8401 ttcctgaaga aaaagctcga ttttctaggc tcttattaga atatcgaaaa agtggtgaag
     8461 tacccttttc aactgaatac ctgtccgatc atctcatcga ctttttctat aaaatgaaag
     8521 cctttgaatt tgaaatcgca ccagaacaag tccgagattt tctaaaaaaa agcctgcagg
     8581 aagaccatcg ctcatcagca caaggaagct ggatagaagg aattctcctc aactgcctca
     8641 aaccattttt agaacgatta gtgatatgag taacacctac cgtgtaaatt tcatggtagg
     8701 tgttattgta cctcaaaa
//
LOCUS       DAVLXF010000078         8637 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLXF010000078 DAVLXF010000000
VERSION     DAVLXF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.078"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            29..1030
                     /locus_tag="VYO12_001699"
     CDS             29..1030
                     /locus_tag="VYO12_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1588261.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1635..1712
                     /locus_tag="VYO12_001700"
                     /pseudo
     CDS             1635..1712
                     /locus_tag="VYO12_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2213..3574
                     /gene="trpE"
                     /locus_tag="VYO12_001701"
     CDS             2213..3574
                     /gene="trpE"
                     /locus_tag="VYO12_001701"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1588262.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3571..4137
                     /locus_tag="VYO12_001702"
     CDS             3571..4137
                     /locus_tag="VYO12_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1588263.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4148..5152
                     /gene="trpD"
                     /locus_tag="VYO12_001703"
     CDS             4148..5152
                     /gene="trpD"
                     /locus_tag="VYO12_001703"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1588264.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5149..5916
                     /gene="trpC"
                     /locus_tag="VYO12_001704"
     CDS             5149..5916
                     /gene="trpC"
                     /locus_tag="VYO12_001704"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1588265.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNTGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5903..6502
                     /locus_tag="VYO12_001705"
     CDS             5903..6502
                     /locus_tag="VYO12_001705"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1588266.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6480..7703
                     /gene="trpB"
                     /locus_tag="VYO12_001706"
     CDS             6480..7703
                     /gene="trpB"
                     /locus_tag="VYO12_001706"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1588267.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7696..8472
                     /locus_tag="VYO12_001707"
     CDS             7696..8472
                     /locus_tag="VYO12_001707"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1588268.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8444..8545)
                     /locus_tag="VYO12_001708"
                     /pseudo
     CDS             complement(8444..8545)
                     /locus_tag="VYO12_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
BASE COUNT         2566 a         1608 c         2069 g         2394 t
ORIGIN      
        1 attagaatgt ttgtaataga ggactgaaat gaaaaatata aatgggaaaa aggtaactat
       61 ttatgatatt gcacgtttat caggtttttc tccaaaaaca gtttcacgag ttattaatgg
      121 tggagtaaat gttaaagaag aaacttatca agcgattcaa aaagttatag aggaattgtc
      181 gtatattcca aatgcgtatg caaaaaattt aactaaaaaa gaagctataa atatattgat
      241 ttctgtaaaa aaaatcgact cttttccttt aatttggttt catacgctgt tagataaagt
      301 gttacggacg tgtaaagaat ttggtgttaa tgctatagtt gagtactttg gagaggagga
      361 tacaattagt aattcaatta tatcaagcac aggtagccta gtagatggtg ttattgtttt
      421 ttatgaaagt gtagatgata tcagaattca gtatttaaag aagaaccata tgccttttct
      481 tgttttcggt gaatctcaaa cgtctggagt agtctatgta tctaataata attttcaagc
      541 tacttatgat atgatgaaag ctgtaactga agaaaaattt aaaaatatgt ggttgcttat
      601 gggaggagaa tcccatgtta ataaggatcg tgaaagaggt gtgcgttctt ttctgaatga
      661 taaaaattac tttatggatt taaaagtcat ttatggctta tctacaattg attcagttta
      721 ttcttatgca atgcagcact tgactaccca aaattatccg gacattattt ttgtttcagg
      781 agatgaaaag gttcagggct tgattcgtgc gtgctatgag aaaggaattc ttattccaga
      841 tgatatttct atcattggat ttgataatat tcctatttct cagtattata ctccagcttt
      901 gtctacaatt gctcccaatt atgttaaatt ggctaaagaa atgatagaag gtgttttagc
      961 aattattaaa ggggaaagtg tcacatctgt tgaagtttct cctaaatttg ttaggagaca
     1021 gagtttctag tattgtttaa ttcttgaaaa tcctagtgga aaagatggag tgttctaaaa
     1081 taagttctgt ttagcctcca ttcagtttct attgagttgg atccaatttc taacaagtgg
     1141 gacatgttca tgttcgatct aaggaagata tggtcacatt cgaatgatat caaaaaaatc
     1201 gtaaaaaagt cttgacaaag aaaaaacaaa gtattaaaat aagttcaaca aatcagaaaa
     1261 gtaactattt ttgatcttca gggagcctgt ggtgattgtg aataggtggt tggaagtagt
     1321 gaaagtgggc tgattttgaa aatgaatttg aaacaatgaa aattcggtgc gcacacctta
     1381 cagtgcagct tgttgttaaa caaggcagag atgtaaaggg atagtccctt tataattgag
     1441 gtggcaccgc gttaccaacg ccctcacacg gaagtatatt ctgtgtgtgg gcttttttct
     1501 atccgtcgtt tggtttatct tttattaggg ccttaagtcg ctttgatgaa cttgagttct
     1561 atctacagct ccttgcctcc tactaaaagt aaacaaaaag gctggactaa tacgaaaaaa
     1621 gctagtaata ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggttgctcaa
     1681 aacactgttt tgaggttgtg gatagaactg acgaagtcag ctcaaaacac tgttttgagg
     1741 ttgcagatag aactgacgaa gtcagtaacc atacctacgg caaggcgacg ttgacgcggt
     1801 ttgaagagat tttcgaagag tataaaggtg taccgagatg aacatctctt catcaatgtc
     1861 tctggtcgtg gaggcaagga cctagctgcg attgcagatt actagaagct aaaaaataat
     1921 agatggaaaa attacagtct tttctctcac agagagcttg tggttgctga aaacaagcag
     1981 agaaagctgt aaagttgggt tcatctgaaa cgagagaaga aaacatcatt ctcaagggag
     2041 tgccctttat cgcacagcct gttacaggag atatctgaga caggaatgag agataggaga
     2101 aatcctataa ttgaggtggc accgcgaatt tcgtcctcac gcaagttatt ttgcgtgggg
     2161 atttttcata cagtcgccac gaactagaat tagaactgaa agggaaatga caatggaacg
     2221 aatcattcat ggagatgtct tatcaccaat cttggcttat atgcgcttaa aggggcaaca
     2281 taaggttatt ttagagagta ttccgagaga caaggaaaca gctcgttttt ctatcctagc
     2341 ctataatcca gtttttgaga ttaagtttga aaatggagtt ctctatcaaa atggtcaagc
     2401 gattgatcgg gatcccttgg atttccttta tgaagtgact cataagagcc agcaccattc
     2461 agacctccct tttggtgggg gagccattgg ttttgttggt tacgatatga tttcgcttta
     2521 tgaagaaatt ggtcaaatcc ctgaggatac aattgggacg ccagacatgc atttctttgt
     2581 ctatgagagc tatatggtct ttgaccacaa gaaggagaag atacatgtca tagaggatgc
     2641 actttatagt gagcgcagtc aagaggcctt ggaaaaatcc ttgaaccaag tgcttgagga
     2701 attacgcatt cctgctccaa atgaatttga agacttggat ttatcaccac ttgacttcaa
     2761 accgcatatc gctccacata agtttgaggg aatggtagaa acagctcgtg acttgattcg
     2821 taacggagat atgttccaat gtgtactcag ccagcgtttc tcagcagaag ttactggaaa
     2881 tccatttgac ttctacagaa atctccgcgt gactaatcca tctaattacc tttatttcta
     2941 tgattttggg gattatcaaa tcatcggagc tagtccagaa agtttggttt ctgtcaaaaa
     3001 tggcatcgtg acaaccaatc cgattgcagg gacgcgacca agaggggcta cggatgaaga
     3061 agacaaggcc ttggcgactg acctcctctc tgatgagaag gaaacagcag aacatcgaat
     3121 gttggtagac ttggggcgta acgatattgg ccgcatctct gaaacggcca gtgtccaagt
     3181 tactaagtat atggaggtgg agctcttccg ctacgtcatg catttgacca gtgtggtcaa
     3241 gggacgtttg cttgcagaac tcactgccat ggatgccttg aaagcaacgc ttcctgctgg
     3301 aaccgtttct ggagcaccaa aaattcgagc tatgagacgc atctatgaac tggaaacgga
     3361 aaaacggagc gtatatgcag gagcaatcgg ctacttgtct gcgacgggtg atatggattt
     3421 ggccattgcc atccgaacca tgattctcaa aaatcaaaga gcctatgtgc aggctggggc
     3481 agggattgtc tacgactcta tcgcccaaaa cgaataccaa gaaaccatta acaaagcaaa
     3541 atctatgact agaattggag aactaagacc atgattttat tgattgacaa ctatgattct
     3601 tttacctata acttggccca atacattggg aattttgcag aagttcaggt tttgagaaat
     3661 gatgattcca agctgtatga agaagctgaa aaagcagatg gtctggtctt ttctcctggt
     3721 cctggttggc cagttgatgc tggaaagatg gaagacatga tttgtgattt tgcaggcaag
     3781 aagccgattc tagggatttg tttgggtcac caagccattg cagaagtctt tggtggtaag
     3841 ctaggtttgg ctccaaaagt catgcatggg aaacagagca atatcaactt tgaagcgcca
     3901 tctgttttgt atcaaggtat tgaggatgga cgtgcggtca tgcgttatca cagtattttg
     3961 attgaggaaa tgccagaaga ctttgaagtg acagctcgtt cgactgatga ccaagccatc
     4021 atggggattc aacataaaaa cctaccgatt tatggcttcc agtaccatcc agagagcatt
     4081 ggaacgccag acggcttgtc ttctattcgg aattttatcg agaaggttgt aaagtgagga
     4141 aactaggatg aaagagatta ttgaaaaact agcaaaattt gaaaatttat caggtgtgga
     4201 aatgacggat gtcattgagc gtatcgtaac tgggcgtgta actgaagcgc agattgcttc
     4261 tctcctctta gctcttaaga tgaaggggga aacacctgaa gaacgtacag ccattgccca
     4321 agtcatgaga ggacatgccc agcatattcc aactgagatt catgatgcta tggacaactg
     4381 tggtacaggt ggggacaagt ctttcagttt taatatttcc acaactgcag cctttgtctt
     4441 ggctggtggc ggtattcaca tggccaagca tggtaaccgc tcgatttctt ctaaatctgg
     4501 ttccgcagat gtcctcgaag ccttgggaat caatcttgac ctcaaaccag ctgaactagg
     4561 taaggtcttt gataaaactg gaatcgtctt tctcttcgct aaaaatatgc acccagctat
     4621 gaaatacatc atgccagctc gtttggaatt gggaattcca acgattatga acttgactgg
     4681 tccactgatt catccaatgg ctttagaaac acagcttctt ggaattagtc gtccagaact
     4741 cctagaaagt acagctcagg ttttgaaaaa tatgggtcgc aaacgtgcca tcgtggttgc
     4801 tggaccagaa gggttggatg aagctggctt gaacggaaca accaagattg cacttcttga
     4861 aaatggcgaa atcagcttgt caagctttac tccagaggat ttgggaatgg aagactatgc
     4921 tatggaagat attcgtggtg ggaatgctca ggaaaatgca gaaattttgc ttagcgttct
     4981 gaaaaacgaa gcaagtccat tcttggaaac gacagtcttg aatgctggtc ttggtttcta
     5041 tgctaatggt aagattgata gcatcaagga aggagttgcc ttggcccgtc aagtgattgc
     5101 tagaggcaag gcccttgaaa aactcagact gttacaggag taccaaaaat gagtcaggaa
     5161 tttttagcac gaatcttaga gcagaaggcg cgtgaggtgg agcagatgaa gctggagcaa
     5221 atccagcctc tgcgccagac ctatcgcttg gcagaatttt tgaagaatca tcaggactgc
     5281 ttgcaggtaa tcgctgaggt caagaaagct agccctagtt tgggagatat caatctcgat
     5341 gtggatattg tgcaacaggc ccagacttat gaagaaaacg gagcagtgat gatttcggtg
     5401 ttgacagatg aggttttctt taaagggcat ttggattatc tacgggaaat ttccagtcag
     5461 gtagagattc cgacgctcaa caaagacttt atcatagatg aaaagcaaat catccgcgct
     5521 cgcaatacag gtgcgacagt tatcttgctt attgtggcag ccttgtccga agaacgcctc
     5581 aaggaactgt atgactacgc gacagagctt ggtctggaag tcttagtgga gactcacaat
     5641 ctagctgaac tagaggtagc ccacagactt ggtgctgaga ttatcggggt caacaaccgc
     5701 aacttgacta cctttgaggt cgacttgcag accagtgtag atttagcccc ttactttgag
     5761 gaaggtcgct attacatttc tgaatctgcc attttcacag ggcaggatgc ggaacgacta
     5821 gccccatact ttaacggaat tttggtaggg acagctctta tgcaggcaga aaatgtggcc
     5881 cagagaatca aggagttgca gattgacaaa ggttaaaatt tgtggactat cgaccaaaga
     5941 agcggtggaa acagccgttt cagcaggagc cgactatatc ggttttgtct ttgcacctag
     6001 taaaagacag gtgactttag aagaggcagc tgagttggca aagcttattc ctgcagatgt
     6061 gaaaaaggtt ggagtatttg tttcaccaag tcgggtagaa ctgctggaag cgattgacaa
     6121 agttggcttg gacttggttc aagttcacgg tcaggtggca gctgatttat ttgagaattt
     6181 gccttgtgcc agtattcagg ctgtgcaggt agatggaaat gggcatgtcc ccaattctca
     6241 ggcagattat ctactctttg atgcccctgt ggcaggaagt ggtcagtcct ttgattgggg
     6301 tcaactggat acgactggac tagcacagcc cttctttatc gcaggtggcc ttaatgaaga
     6361 taatgtagta aaagcaattc aacattttac tccctatgca gtagatgtat cgagcggagt
     6421 ggagacagat ggacaaaaag atcatgaaaa gattagaaga tttatagaga gggtaaagca
     6481 tggcatatca ggaaccaaat aaagatggat tttacggaaa attcggcgga cgttttgtcc
     6541 cagaaacatt gatgacagca gttttggagt tggagaaggc ctaccgtgaa agtcaggcag
     6601 acccaagttt ccaagaggaa ttaaaccagc ttttgcgcca gtacgtagga cgtgaaactc
     6661 ctctttatta cgcaaaaaac ttgacccagc atatcggcgg agccaagatt tatctcaaac
     6721 gggaagacct taaccataca ggagcccaca agattaacaa tgccttggga caagtttggc
     6781 ttgccaaacg catgggtaaa aagaaaatta tcgcagaaac tggtgctggt cagcacggtg
     6841 tggcaactgc aactgctgcg gccctcttta acatggaatg taccatctac atgggtgagg
     6901 aagatgtcaa acgccaagcc ctcaatgtct tccgtatgga gcttttggga gctaaggtcg
     6961 aggccgtgac agatggttcg cgcgtgctca aggatgcggt caatgcagct cttcgttcat
     7021 gggtggctaa tatagatgat acccactata tccttggttc tgccttgggg cctcatccat
     7081 ttccagaaat tgttcgtgac ttccaaagtg tcattggtcg agaggctaaa caacagtacc
     7141 gtgacttgac aggacaaaat ctgccagatg ccctagtagc ctgtgttggt ggtgggtcga
     7201 atgccattgg tctcttccat ccctttgtag aagatgagtc agtagccatg tatggagctg
     7261 aagcggctgg acttggtgtg gatacggagc accacgcagc taccttgacc aagggtcgtc
     7321 caggtgtcct tcacggttcc ctcatggatg tgctccaaga tgcccatggt caaattcttg
     7381 aagccttctc tatctcagca ggtttggact atcctggtat cggtccagag cattctcact
     7441 accacgatat taaacgtgcc agctatgtcc ctgtgacaga cgaagaagcc ttggaaggat
     7501 tccaactctt gtctcgtgtg gaagggatta tcccagcctt ggaatctagc catgctatcg
     7561 cctttgcggt gaaattggcc aaagaacttg gaccagaaaa gtctatgatt gtctgcctat
     7621 caggtcgtgg ggacaaggat gtggttcaag tcaaagaccg cttggaagca gatgcagcaa
     7681 agaagggaga agctcatgcc taagacacta acaaaaaaat taaatgctat taaagcagct
     7741 ggaaaaggaa tttttgttcc ctatatcatg gctggtgacc acgaaaaagg tttggatggt
     7801 cttgctgaaa caattcactt tttagaagat ttgggtgtct ctgcaattga agtgggtatt
     7861 cccttttcag accctgttgc agatggacct gttatcgaag aagctggctt gcgcagttta
     7921 gcccacggga cctctaccca ggctttagtt gaaaccttga aaaccattga aacagagatt
     7981 ccactggtca tcatgaccta cttcaacccc ctctttcagt acggtgtgga gaactttgtc
     8041 aaagatctgg ctgatacagc tgtgaagggc ttgattatcc cagacctgcc tcatgaacat
     8101 gccaactttg tagaaccatt tttggcagat acagatatcg ccttgattcc tttagtaagc
     8161 ttgaccacag gaattgagcg ccagaaagag ttgattgaag gggcggaggg attcgtctat
     8221 gccgttgcca tcaatggggt aacagggaaa tcaggcaatt accgtgcaga tttggacaag
     8281 cacttggcgc aacttcatca agtggccgac atcccagtct tgacaggttt tggtgtatct
     8341 agtcaagccg atctggaacg cttcaatgcg gtgtcagatg gtgttatcgt cggttcgaaa
     8401 atagtaaaag ctctccacca aggagagccg attcaggact ttatcaaaca agcagtagct
     8461 taccaaaaat aattaaacaa gtagcaagta agaggaaagg cacgaaagga agtctttcct
     8521 ttttcttttg caggcgaaag gctagtttta tagtgaattg aatctggaat attacactac
     8581 ggttcctaaa atattttaag aaagtgattt gaatttccca ataaatttgt tcagttt
//
LOCUS       DAVLXF010000079         8536 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLXF010000079 DAVLXF010000000
VERSION     DAVLXF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.079"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            161..2794
                     /gene="polA"
                     /locus_tag="VYO12_001709"
     CDS             161..2794
                     /gene="polA"
                     /locus_tag="VYO12_001709"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1588269.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNMSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDQLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEIKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2879..3316
                     /locus_tag="VYO12_001710"
     CDS             2879..3316
                     /locus_tag="VYO12_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1588270.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(3501..4511)
                     /locus_tag="VYO12_001711"
     CDS             complement(3501..4511)
                     /locus_tag="VYO12_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1588271.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVINAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            4660..5829
                     /locus_tag="VYO12_001712"
     CDS             4660..5829
                     /locus_tag="VYO12_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1588272.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLKDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            5826..6596
                     /gene="recO"
                     /locus_tag="VYO12_001713"
     CDS             5826..6596
                     /gene="recO"
                     /locus_tag="VYO12_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1588273.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            6593..7585
                     /gene="plsX"
                     /locus_tag="VYO12_001714"
     CDS             6593..7585
                     /gene="plsX"
                     /locus_tag="VYO12_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1588274.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIVGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            7591..7824
                     /locus_tag="VYO12_001715"
     CDS             7591..7824
                     /locus_tag="VYO12_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1588275.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            7870..8160
                     /locus_tag="VYO12_001716"
                     /pseudo
     CDS             7870..8160
                     /locus_tag="VYO12_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8363..>8536
                     /gene="blpU"
                     /locus_tag="VYO12_001717"
     CDS             8363..>8536
                     /gene="blpU"
                     /locus_tag="VYO12_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV1588276.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATG"
BASE COUNT         2476 a         1545 c         2151 g         2364 t
ORIGIN      
        1 gtgtactatt atagattcaa tatactatat tatagcacat ttttaaatct aggtttacta
       61 aattctctgg ctctatctat ttattcggaa aaagtttgaa aaatacttgc tctagctctt
      121 cccaatggta ttttttgatt ctttccttta taatgggtgt atggataaga aaaaattatt
      181 attgattgat gggtcttctg tagcttttcg ggcgtttttt gcgctgtatc agcagttgga
      241 ccgttttaag aatgcggctg gtttgcatac caatgcgatt tatggttttc agttgatgtt
      301 gagtcatttg ttggagcggg ttgagccgag tcatattttg gtggcttttg atgcgggaaa
      361 gacgaccttc cggacagaga tgtatgcgga ctataagggt ggtcgggcca agactcctga
      421 tgagtttcgt gagcaatttc ctttcattcg tgagttgctg gatcatatgg ggattcgtca
      481 ctatgagctg gctcagtatg aggcggatga catcattggg acgctggata agctagcaga
      541 gcaggatggt tttgatatta ccattgtcag tggggacaag gatttgattc agctgacgga
      601 tgagcatacg gtggttgaaa tttccaagaa aggtgtggct gagtttgagg cctttacgcc
      661 agattacctc atggaagaaa tgggcctcac accagctcag tttatcgatc tcaaggcgct
      721 catgggtgat aagtcggata atatccctgg ggtgaccaaa gtcggtgaaa agacgggtat
      781 taagctcttg ctggagcatg gttcgcttga ggggatttat gaaaatattg atggaatgaa
      841 gacttctaag atgaaggaaa atctcatcaa tgacaaggaa caggcctttt tgtctaaaac
      901 actagcgacc attgatacca aggcaccgat tgcgattggt ttagaggact tggtctatag
      961 tggtccagat gttgaaaatc ttgggaaatt ctacgatgag atgggcttca aacagctcaa
     1021 gcaggcttta aatatgtcgt cagctgatgt ggctgagggt ttggatttta ctattgttga
     1081 ccaaatcagt caagatatgc tgagtgaaga gtctatcttc cactttgagc tttttggtga
     1141 gaattaccat acagataatt tggttggatt tgcctggtct tgtggggatc agctctatgc
     1201 cacagacaag cttgagctgt tgcaagaccc gattttcaag gatttcttag aaaaaacatc
     1261 tctgagagtt tatgacttta agaaggttaa agttcttttg caacgttttg gtgtagattt
     1321 gcaggcgcct gcttttgaca tccgtttggc taaatacctc ctttcgactg tggaggacaa
     1381 tgaaattgcg accatcgcta gtctttatgg tcagacttac ttggttgatg atgaaacttt
     1441 ctacggtaag ggtgttaaaa aggccattcc tgaacgtgag aaattcttgg aacacttagc
     1501 ttgtaaactt gctgttttgg tagaaacaga gcctatttta cttgaaaaac ttagcgaaaa
     1561 tgggcaatta gagcttcttt atgatatgga gcaacctctg gcttttgtcc ttgccaagat
     1621 ggaaattgct gggattgtgg tcaagaaaga gaccttgctt gagatgcagg ctgaaaatga
     1681 gcttgtcatt gaaaaactga ctcaagagat ttacgagctg gctggtgagg agtttaatgt
     1741 caactcgcct aagcagttgg gcgtgcttct ctttgagaaa ttgggacttc ctctagaata
     1801 cactaagaaa accaagacag gttattcgac agcagtggat gtgttggagc gtctcgctcc
     1861 tattgctccg attgttaaga aaatcctgga ttaccgtcaa attgctaaga ttcaatctac
     1921 ttatgtaatt ggcttgcagg actggatttt ggctgatgga aagattcata ctcgctatat
     1981 gcaggatttg acccagaccg ggcgtttgtc tagtgtggat ccaaacttgc aaaatattcc
     2041 tgcccgattg gaacaagggc gcttgattcg gaaggctttt gtgccagagt gggaggatag
     2101 tgtgctactc agctctgact attcacagat tgaattgcgc gttttggcgc atatttctaa
     2161 ggatgagcac ttgattaagg ccttccaaga gggggcagat atccatactt cgacagccat
     2221 gcgggtcttt ggcattgagc gtcctgataa tgtgactgca aatgaccgtc gcaatgccaa
     2281 ggcagttaac tttggagtgg tttatgggat ttcagacttt ggcttgtcta ataatttggg
     2341 aattagtcgt aaggaagcca aagcctacat tgatacctac tttgaacgtt ttccaggtat
     2401 taaaaactac atggatgaag tggtgcggga ggcgcgtgat aagggctatg tagagaccct
     2461 ctttaagcgt cgccgtgagt tgccagatat caattcgcgc aacttcaata ttcgtggttt
     2521 tgcggagcga actgctatca actcacctat ccagggttcg gcagcagata ttctcaagat
     2581 tgccatgatt cagctggata aagccttggt tgcaggtggt tatcagacta agatgctgtt
     2641 acaagtgcac gatgaaatcg tccttgaagt gcctaaatct gaattggtag agataaaaaa
     2701 attggtgaaa caaaccatgg aagaagccat tcaactcagt gttcctctta tcgcagatga
     2761 gaatgaaggg gcaacctggt acgaggctaa ataaaaaggg ggctagtcct ccttttttgt
     2821 agtagaattc tgcaaacctt ttcaaaatgt gctatactga tgaaaaagga ggatttctat
     2881 gagtcaagaa tttatcaatc caagtgatgg cgtgattcgt cagtatctcg caacgagtaa
     2941 aacccttgct gtggtgggtt tgtctgaccg tgaagaaaca accagcaatc gagtaacaaa
     3001 ggaaatgcag gctcggggct ataaaatcat cccagtcaat cccaaggcgg caggtggcga
     3061 aatcttgggt gaaaaggctt atgctagcct cgctgaaatt ccttttcctg tagatattgt
     3121 caatgtttat cgtcgcagtg aatttctgcc cgatgtggcg cgtgattttc tcaaagctga
     3181 tgccaagatt ttttgggcac agctaggact tgaaagttta gaagcaaaag agattttgcg
     3241 tgatggtgga tgtgatgata tcgtgatgaa tcgctgtatc aagagagaac atactcgctt
     3301 gattgaggaa gcataagaaa aaggtagctg atgggctacc ttttgtgtta tactcaatga
     3361 aaatcaaaga gcaaactagg aaactagctg cgggttgctc aaaacaccgt tttgaggttg
     3421 cagatagaac tgacgaagtc agtaacatat acctacggca aggcgacgtt gacgtggttt
     3481 gaagagattt tcgaagagtg ttagaaaatg ccgataaggg tctgcatacc aagagtggtg
     3541 aggatgatgg caatccagca gatggctcca agaagaatgg attttccact ggatttgacc
     3601 atagcgacca gattggtttt gagaccgata gcactcatgg ccatgacaat aaggaattta
     3661 gagagttctt tgagaggggt aaagaaacta ctggacacac ctagagaggt gagtagtgta
     3721 gtgaggagag aggcaaggat aaagtaaagg ataaaaagtg ggaagacttt tttcagttgt
     3781 aggctttgct tgttttcttg ttggcgactt tgccagtagg atagaaagag cgtgataggg
     3841 ataatggcca aagtacgtgt gagtttaaca atggttgcag actcgagggt attgctttgg
     3901 taaagactgt cccaagcgct ggcggcagcc gttacagagg aagtgtcgtt gaccgcagtc
     3961 cctgcaaaga gggcgaagcc ttcattggat agatgaagcc aggtgccgag ggttggaaag
     4021 atgagcgcag ccaagacatt gaagaaaaag ataacggaaa tggcttgggc tacttccttt
     4081 tccttagcat taataacggg cgctgtcgct gcaatggcag aacccccaca gatagaagaa
     4141 cctactccaa ccaaggtagc cagttttgta tccagtgcaa agaaacgctg gaagaggtag
     4201 gcaataatca gagctattga gatagtggac aggatgacag ggagtgaaga ttggccaact
     4261 gcgaagacct gcgagatatt gagaccaaaa ccaagcaaga caacggcata ttggagcaac
     4321 ttcttggaac taaaggtcaa accagcatcc agttgtttat aggacgagag aaagggatgt
     4381 aggagcatgc ctatgaaaat ggcaaaaacg ggcgcgccaa tcacagggaa gaatcctcct
     4441 aagtaccaag atacgataga aatgagaagg caggccaaga tacctgctcc attttttgat
     4501 agaaatgaca taaaaacctc caaataaaat ctattaccat tatagaccta taaacaggaa
     4561 aagtaaaata gaaagtggaa agctattctc taatgtattt ttgcggtcgg gaggcttttg
     4621 tagtataata gagatacgtt ttgaaagtag gaggtatcta tggacttaac taagcgcttt
     4681 aataaacagt tagataagat tcaagtttcg ttgattcgtc agtttgacca ggctatttcg
     4741 gagattcctg gggtcttgcg tttgaccttg ggggaacctg attttacaac gccagaccat
     4801 gtcaaggagg cggccaagcg agcgattgat cagaaccaat cctactatac agggatgagt
     4861 ggtctgctga ctctacgtca ggcagctagt gactttgtta aggaaaagta ccaactggac
     4921 tatgctcctg aaaatgaaat cttggttaca attggggcga cagaggcttt atctgcgact
     4981 ttgacggcta ttttggaaga gggagacaag gtacttttgc cagctcctgc ttatccaggc
     5041 tatgaaccga ttgttaactt agttggggca gaaattgttg agattgatac gactgaaaat
     5101 ggttttgtct tgactcctga gatgttggag aaggccattt tggagcaggg tgataagctc
     5161 aaagcggtta ttctcaacta tccagccaat ccgacaggaa ttacctacag tcgagagcag
     5221 ttagaggcct tggcagctgt tttacgcaag tacgaaattt ttgttgtctg tgatgaggtt
     5281 tactcagaat tgacctacac aggcgaagcc catgtgtctc taggaacgat gttgaaagac
     5341 caggctatta ttatcaatgg tttgtctaaa tcgcatgcca tgacaggttg gcgtttgggg
     5401 ctgattttcg ctcctgcggc cttcacagcc cagttaatca agagtcacca atacttggtc
     5461 actgccgcaa ataccatggc gcaacatgct gcggtagaag ccttgacggc tggtaaaaat
     5521 gatgcggagc ccatgaagaa ggaatatatc cagcgtcggg actatatcat cgaaaaaatg
     5581 actgctcttg gttttgagat tatcaaacca gacggtgcct tctatatttt tgctaaaatt
     5641 ccagcgggct acaatcaaga ctcctttgct tttctgaagg attttgctca gaagaaggcc
     5701 gttgccttta tccctggtgc agcctttgga cgttacgggg aaggctacgt ccgcctatct
     5761 tatgcagcca gcatggagac tatcaaagaa gccatgaaac gacttgagga gtacatgaga
     5821 gaagcatgat tcagtctatc acgagtcaag gcttggtgct ttacaatcgc aattttcgtg
     5881 aggatgacaa gctcgtcaaa atttttacag agcaggttgg caaacgcatg ttttttgtca
     5941 aacacgctgg tcagtctaag ctggcgcctg ttattcagcc cttggtgttg gcacgatttc
     6001 tcttgcgaat caatgatgac ggactcagtt acatcgaaga ctatcatgag gtcatgactt
     6061 ttcccaagat taatagtgac ctctttgtca tggcctatgc gacctatgtg gcagctcttg
     6121 cagatgctag tttgcaggac aatcagcagg atgctccctt gtttgctttt ttgcaaaaga
     6181 ctttggagtt gatggaagca ggcttggatt atcaggtttt gaccaatatt tttgaaattc
     6241 aaattttgac tcgatttgga atcagcctca attttaatga gtgtgtcttc tgccatcggg
     6301 ttggtcaggc ttttgacttt tctttcaaat atggagcctg cctctgtcca gagcattatc
     6361 atgaggataa gagacgttgt catctcaatc ccaatatccc ctatctgctc aatcaatttc
     6421 aagctattga ttttgagact ttggagacca tttcgctcaa gcctggaatc aagcaagagc
     6481 tacgccaatt tatggatcaa ttatatgaag agtacgttgg gattcaccta aaatcaaaga
     6541 aatttattga ttccctagca gactggggac aattactaaa agaggaaaag aaatgaaaaa
     6601 aatcgcagta gatgccatgg ggggcgatta cgcacctcag gccattgttg agggtgtcaa
     6661 tcaagcccta tctgactttt cagatatcga ggttcaactt tacggagatg aagctaaaat
     6721 caagcaatat ctgacagcga cagagcgcgt cagcattatc catacggatg agaagattga
     6781 ttcggatgat gaacctacga gagctattag gaataagaaa aatgccagta tggtattggc
     6841 agccaaggct gtcaaagatg gtgaagcaga cgctgtcctt tcggctggga atacaggtgc
     6901 cttgttggca gcaggattct tcatcgtggg tcgtatcaag aatatcgacc gtcctggact
     6961 catgtctacc ttgcctaccg ttgatggaaa aggttttgac atgctagacc ttggtgccaa
     7021 tgcagaaaat acagcccagc acctccatca atatgcggtt ctaggttcct tctatgctaa
     7081 aaatgtccgt ggcattgcgc aaccacgcgt tggtttgctc aacaacggaa cagagagtag
     7141 caagggcgac ccgcttcgta aggaaactta tgaattactg gcggctgatg aaagtttgaa
     7201 ctttatcgga aacgtggaag cgcgtgattt gatgaatggc gttgcagatg ttgttgtggc
     7261 agatggtttc acgggaaacg ctgtgctcaa atccatcgaa gggacagcta tgggaatcgt
     7321 gggcttgctc aagacagcta ttacaggtgg tggtcttcga gcgaaactag gtgccctcct
     7381 tctcaaggac agcctcagtg gtttgaaaaa acagctcaat tattcagatg ttggtggagc
     7441 ggtcttgttt ggtgttaagg cacctgttgt caagactcat ggctcaagcg atgccaaggc
     7501 tgtttatagt acgattcgtc agattcgtac catgctagaa acagacgtgg ttgcccagac
     7561 tgcgcgtgaa ttttcaggag aataaaagag atgacagaaa aagaaatttt tgaccgtatt
     7621 gtgaccatta tccaagagcg acagggagag gactttgtcg tgacagaatc cttgagtctg
     7681 aaagacgatt tggatgcgga ttctgttgac ttgatggagt ttatcttgac tctggaagat
     7741 gaatttagta tcgaaatcag cgatgaggaa attgaccaac tccaaagtgt aggagatgtg
     7801 gttaaaatca ttcaaggaaa atagcaatcg gagttccaag tcaacggaag tagatggttt
     7861 ttagaaatga gaaatattgg acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg
     7921 ctcatgaaga tatatcctca agcacaaact ccacgtaaat ccagcaaact caagccgcta
     7981 acagttgaag ataaagcctg taaccatgcg ctatctaagg agagaagcaa ggttgagaac
     8041 atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa
     8101 cgcttcggat tacgaatgaa tttgattgct ggtattatca atcatgaact aggattctag
     8161 ttttgcagga agtctaatag taaaaaagtg attagaaaac atctttttta aaaatagaga
     8221 tgattttgaa acaaaaaagc taattcaaga cgtttcgatg ccaattcaag atttggatga
     8281 aaaaaattaa tagatactgt tatactaaac ttgtcaagtt tgtaacaaga caaatattaa
     8341 aaataaaaaa gaggtattcg ttatgaatac aaaaaagatg tcacaatttg aaattatgga
     8401 tactgagatg cttgcttgcg ttgaaggtgg cggatgcaat tggggagatt ttgccaaagc
     8461 aggtgttgga ggaggagcag cacgaggtct tcagctagga attaaaacaa gaacatggca
     8521 aggtgcagca actggt
//
LOCUS       DAVLXF010000080         8468 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLXF010000080 DAVLXF010000000
VERSION     DAVLXF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.080"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            82..261
                     /locus_tag="VYO12_001718"
     CDS             82..261
                     /locus_tag="VYO12_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1588277.1"
                     /translation="MAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEIL
                     TGISKSSVSRELSED"
     gene            263..2506
                     /locus_tag="VYO12_001719"
     CDS             263..2506
                     /locus_tag="VYO12_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEV1588278.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLDKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDRVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIAPDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VVLSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            2557..3576
                     /locus_tag="VYO12_001720"
     CDS             2557..3576
                     /locus_tag="VYO12_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1588279.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYKDFYEFQNRTYISRKENEEEYSN
                     LIYESLFSDTVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEIVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDNMRTSLPVINQPAIDKKYNENLYQHDIVNILTLNYT
                     NVIDKLYNESAKSFSNQLRTNNYKFHIAPPIHAHGTVDICTVLGVSDETQISNSFDEE
                     QKESLIKNLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDGYIWNQIAEQSIRSS
                     VPVIIYHYVPHFDAGNPTRVKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIF
                     NLMER"
     gene            3589..4539
                     /locus_tag="VYO12_001721"
     CDS             3589..4539
                     /locus_tag="VYO12_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1588280.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     IFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVVAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            4541..5284
                     /locus_tag="VYO12_001722"
     CDS             4541..5284
                     /locus_tag="VYO12_001722"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1588281.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            5295..7091
                     /gene="pepF"
                     /locus_tag="VYO12_001723"
     CDS             5295..7091
                     /gene="pepF"
                     /locus_tag="VYO12_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1588282.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETA
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(7151..7423)
                     /locus_tag="VYO12_001724"
                     /pseudo
     CDS             complement(7151..7423)
                     /locus_tag="VYO12_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            7411..7593
                     /locus_tag="VYO12_001725"
                     /pseudo
     CDS             7411..7593
                     /locus_tag="VYO12_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
     gene            complement(7717..7806)
                     /locus_tag="VYO12_001726"
                     /pseudo
     CDS             complement(7717..7806)
                     /locus_tag="VYO12_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7803..8336)
                     /gene="queF"
                     /locus_tag="VYO12_001727"
     CDS             complement(7803..8336)
                     /gene="queF"
                     /locus_tag="VYO12_001727"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1588283.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQINREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISINPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT         2623 a         1510 c         1868 g         2467 t
ORIGIN      
        1 ttaaaattat ttaaaaaaat tatcaatagt gccaatgtaa taccgaatcc tggtgatttg
       61 agaaactcaa taagaacgtc attggcattt agtgacccat cagatccgca tgattaccaa
      121 aaacgcctgc taagaagttt gcataatggt agaaatttat ctatttctaa attatcgaag
      181 ctaggtttta ctgttagaga aattgagatt ttgacaggta tttctaagag tagtgtatca
      241 cgagagttaa gcgaggatta aaatgaatac attattgaca ttacgaggta aaagttttac
      301 accaaagtca gcaaagggga ggggaccgat aacgattccc gcagattcta ttgttacgct
      361 aaaccattta aatgaacttt atacatccct tgaacgagta aaagaatatt ggtctaataa
      421 cacaatcatt gatggagtac ttattagtgt gtattataat cgtgtcctag ctaagagcaa
      481 tcgtatcgat ggttttttta atgtgggtgg cggaaatgca attccaaatg aaacgattgt
      541 gggtgcaaaa tttagtaatg ataagagtag gcatattatt acacactaca tttcaaagga
      601 agtgttagat aagacaattg agcatactaa aaaagttata acaatattca aagattattt
      661 tgctagtgct cccatgacga gtatggtgtt ctccgactct tcaaaattta atcaaattga
      721 ttattctaag tatggtctgt cgaaatcaaa attcaaacaa tacttaagag acgcttgctt
      781 tgtcaaagga tttggtattg aacaggcaga tatgtctact atacgtaatt ctattgtaac
      841 aatctacgat gtaaaaacta atctcttaac attacttaaa caacttgata ttgatgtttc
      901 agaagctaac atcatggacc agacaactgt tttacttgac gaaaaaaata ttgagttact
      961 actgctaaaa gcaccttatc tagtatcaat gatagtagaa gatttttcta gcctgtcacc
     1021 agagaatttt tataatgatg ggaacacttt ttataataat ctcccttttc caactaatga
     1081 acctattatt ggtgtcatcg atacgttgtt tgatgataga gtttatttca atgagtgggt
     1141 agagtatcat gatttaattt caccagatat tagcaaagga agtcaagact ataagcacgg
     1201 tacagcagtt tcctcggtaa tagtagatgg agctagtttg aatccaaaac tagatgacgg
     1261 atgtggaaga tttagagtca gacattttgg gatatcttta cagagtggtt tcaattcctt
     1321 cacaattatc aaacagatta gagaaattgt gtctcaaaat cctgatatta aggtttggaa
     1381 cctttcgctt ggatcaaatg atgaaattag ggaaaacttt atttctgtcg agggttcgtt
     1441 gttagacgag attcaatttg ataacgatgt aatttttatt attgcaggca ctaatgggat
     1501 tgcccctgat ggacaacgaa aaaagattgg agcaccagca gattcactca attcaattat
     1561 tgtaaattct gttgatttta gcaatcaagt cgtttcttat tcacgcgaag gagttgtcct
     1621 gtctttcttt atcaaacctg atgtttcata ctatggcggg gggaacggag acttcataaa
     1681 tgtttgtgaa ccactagggc tggctcgagt agctgggacc agttttgcgg ctccatttat
     1741 tgctagaaaa atggcttacc tcattcatat tatgggatta agtcgtgaag aagcaaaggc
     1801 tttattaatt gattcagcga taccatggaa tgataaaaaa acattcgcag acatttcttt
     1861 acttggatat ggggttgtgc caatcaaaat agaagatatt ttgtctacac ctgatgatga
     1921 aattaaattt atagtctcag atgtttctag agcatatgaa acttataatt atgatttccc
     1981 agttccaatt tctaaagaac actatccata tgtagcaaag gccactatgt gctattttcc
     2041 aaagtgttct agaaatcaag gagttgacta tactaatacg gaaatgcaac ttagtttggg
     2101 agttttagat aagaaaggca ttaaaacaat taataacgac aatcaatatt ctgaaaatgc
     2161 accaggatat gtgagagaaa agtcggcacg aaatattttt aggaaatggg ataatgtcaa
     2221 acatttagga gaaaaatttt cttctcgtaa aaaagctaag ccgattctga atccttctaa
     2281 tcctcaatgg ggaatgagtg taaaaacaat tgaacggtta agaaatgatg atggttatgg
     2341 tatccgtttt ggagtagttg ttactctaaa ggagttgaat ggtgtcaata gaatcgaaga
     2401 ttttattcaa caagccgaat tacgcggttg gctagttaat agattgcaag tagaagctca
     2461 agtagacctc tttaatagtt taaatgaaga aattgaattt gaatagtgga tattttatcc
     2521 ttattctaga tcttaggaac acaagagggc aaagatatga atattactta tatagttgga
     2581 aatggtttgg atttgcaata tggtttgaaa acaagatata aagatttcta tgaatttcaa
     2641 aataggactt acataagtag gaaagagaac gaggaagaat attcaaatct catttatgag
     2701 tctctatttt ctgatacagt aaatgattat gaaaattggt cagattttga attgtcaatt
     2761 ggtaaattaa caaaagataa cgacctcatc tcctcaagta tagaaataaa ggagaaattt
     2821 atagatgatt ttagtgaaat agttgatgat ttaagagaat atctacgcat acaacaagaa
     2881 aaaaatcttg agaaaggtaa tgcaattgac tttataagca ctctagataa tatgaggacc
     2941 tctctccctg taattaatca acctgctatc gataaaaaat ataatgaaaa tctatatcaa
     3001 cacgatatcg tgaatatatt aactcttaat tacacaaatg taatcgataa attatataat
     3061 gagtcagcaa aatcatttag caatcaatta cgaactaata attataaatt tcacattgcg
     3121 cctccaattc atgcacatgg aacggtagat atttgcacag ttttaggagt tagtgacgag
     3181 acacagattt caaatagttt tgatgaagaa caaaaagaat ccttaataaa aaatttagtt
     3241 ttgaagaact atagagaaaa tatggatgtt aaaaatagcg atattataaa aaattctgac
     3301 atcataattc tttatggggt ttctttggga gagactgatg ggtatatctg gaatcagatt
     3361 gctgaacaat caatcagaag ttctgttcct gtcataattt accattatgt acctcatttt
     3421 gatgcaggaa accccactag agtaaaacgc ttatatagaa atgtagaaga caaattcata
     3481 caaaatagtg gaattgattt agagttagag aagaaattaa gagataacct tattgttgtt
     3541 attggaaaga ctatttttaa tctgatggaa aggtaagcaa aatacataat ggaaacatgg
     3601 caagagttaa aagttacagt gaagcgtgag ggagaggagt tggtttccaa tctcttgatt
     3661 gagctgggag cgcaaggagt cgcaatcgaa gacagtatgg actatgtggg aaatgttgac
     3721 cgctttggcg agattttccc agaggtggag cagcaagaag aaatcgtagt gacagcctac
     3781 taccctgaca cggtggatgt gacagtggtt gaggcggatt tgcaggctcg tctagcagaa
     3841 ttgacagatt ttatggattt gggagagtta aaaataggta cgactgcctt ggctgaggaa
     3901 gactgggcag acaactggaa gaaatactat gaacctgctc gcatcactca tgatttgacc
     3961 atcgttccct cttggacaga ctatgaggcg actgcgggag aaaaaattat caagctggat
     4021 cctggcatgg cttttggtac gggaacccac ccaactacca agatgagcat ttttgccttg
     4081 gaacaggttc ttcgtggtgg cgaaacggtg ctagatgtgg gaactggttc aggggttctc
     4141 tctattgcca gctcgcttct gggggctaaa gaaattttcg cctatgacct ggatgatgtg
     4201 gcagttcgtg tggctcagga aaatattgag ctcaaccctg gaatggaaaa tatccatgta
     4261 gtggcaggcg atttgcttaa aggcgtggag attgaagcag atgtcatcgt ggcaaacatc
     4321 ttggcggata tcctcattca tctgacagag gatgcttatc gcttggttaa ggatgaaggt
     4381 tacctgatca tgagtggcat tatcaaggat aagtgggaca tggtgcgcga gttggctgaa
     4441 tcagctggat ttttcctcga aactcacatg gttcaagggg aatggaatgc ctgtgtcttt
     4501 aagaaaacca aggatatctc tggtgtgatt ggaggctagc atgcagcagt attttgtaaa
     4561 aggcagtgct atctctcctg tcactatcga gaacaaggaa accagtaagc atatgtttca
     4621 ggttatgcgc ttgaaagaag aggatgaggt taccttagtc tttgatgatg gcatcaagcg
     4681 cttggcgcgc gtgctggata tggaaaatcg tcagtttgag ttggtccaag aattaaatga
     4741 caatgtggaa ctaccagtcc aagtgaccat cgcatctgga tttcccaagg gagacaagct
     4801 ggagttcatc gctcaaaaag taaccgaact gggtgctagc caaatctggg cctttcctgc
     4861 agactggtca gttgccaagt gggatggcaa gaaattgggt aaaaaagttg aaaaactaga
     4921 aaaaattgcc cttggagcag ccgagcaaag caagcgtaat attgtaccaa gtattaaact
     4981 tttcgagaaa aaagcagatt ttctagctca gctggaccag tttgactcta tcatagtagc
     5041 ctatgaagaa tcagctaaag aaggagaagc cgctgcgctt ctgcaagcag tcactggtct
     5101 tgaaacagga gccaaactgc tctttatctt tggtccagaa ggcggtctct cacctgcaga
     5161 aatcgaaagt tttgaagcta agggagccgt tttggcaggc ctaggtcctc gtattttgcg
     5221 agcagaaaca gcaccgcttt acgctttatc agcccttagt gttttagtag aattagagaa
     5281 ataagaggaa gaaaatggaa caaaaacacc gttcagaatt tccagagaag gaactctggg
     5341 acttaacagc cctataccaa gaccgtgagg atttcttgcg tgcaatcgag aaagctcgcg
     5401 aagacatcaa ccagtttagc cgtgattaca agggcaatct tcacactttt gaggatttcg
     5461 agaaggcctt tgcggaattg gaacagatct acattcagat gagccatatt ggcaactatg
     5521 cttttatgcc tcagacgacg gactatagca atgacgaatt tgccaatatt gcccaagctg
     5581 ggatggaatt tgaaacagct gccagcgtag ccttgacctt ctttgacgat gccttggtgg
     5641 cagcagacga ggaagtcttg gaccgtttgg gtaaattgcc acatttaaca gctgccattc
     5701 gtcaggctaa aatcaaaaaa gcccactact taggggcaga tgtggagaag gccttgacaa
     5761 atctcggtga agttttctat agtccgcagg acatttatac taagatgcga gctggggatt
     5821 ttgaaatggc tgactttgaa gcccatggca agacctataa aaacagcttt gtcacctatg
     5881 agaatttcta ccaaaatcac gaggatgctg aggttcgtga gaaatccttc cgttccttct
     5941 cagagggact tcgtaagcac caaaatacgg ctgcagcagc ctatctggct caggtcaagt
     6001 ctgaaaaact cttggctgat atgaagggat acgactctgt ctttgactat cttctagctg
     6061 aacaagaagt ggaccgtgtc atgtttgacc gccagattga cctcatcatg aaggactttg
     6121 caccagtcgc tcagagatac ctcaagcatg ttgccaaggt aaatggtctt gaaaagatga
     6181 cctttgcaga ctggaaattg gacttggaca gcgccctgaa tcctgaagtg actattgacg
     6241 atgcctatga tttggtcatg aagtcggtag aacctttggg gcaagaatat tgtcaggaag
     6301 ttgctcgtta ccaagaagag cgctgggtgg attttgctgc taacagtggc aaggattccg
     6361 gtggttatgc ggcggaccca tatcgcgtac acccttatgt actcatgagc tggacaggcc
     6421 gtttgagcga tgtctatacc ttgattcatg aaatcgggca ttctggtcaa ttcatctttt
     6481 cagacaatca ccaaagctat ttcaacgccc acatgtcgac ctattatgtt gaagcaccgt
     6541 caaccttcaa tgaattgcta ctcagtgatt acttagagaa ccagtctaat gacccacgtc
     6601 aaaaacgctt cgctctggct catcgcttga cagacaccta cttccataat ttcatcaccc
     6661 acctcttgga agccgccttc cagcgtaagg tgtatacatt gattgaagaa ggggagacct
     6721 ttggagcaag caagctcaac agcattatga aggaagtttt gacagatttt tggggcgatg
     6781 ccattgaaat cgacgacgat gcagctttga cttggatgcg ccaagctcac tactatatgg
     6841 gcttgtatag ttacacttac tctgctggtc ttgtcatctc gacagctggt taccttcatc
     6901 ttaaaaactc agaaactgga gctgaagact ggctcaatct cctcaaatca ggtggtagca
     6961 agacaccact tgagtcagcc atgattatcg gtgctgatat ttcaacagac aaaccactcc
     7021 gtgataccat ccaattcttg tctgacacag ttgaccagat tatcgcctac agtgctgagt
     7081 tgggggagta aggggataga gaggttgtta tagaatgggt atggacagtt atttgcttaa
     7141 aagtccatgt ttaaatcccc cattcctttt cgaggaattc tttgcccttc tctgtaatgg
     7201 taagtgaacg tggcgtttgt gcagggatga ccaattgttt ttcactgaaa aattctaaaa
     7261 gagcattgcc gagagctccg ccgacatgat ttttacgttc gctccaatcc aaacatttag
     7321 ggcaaaattt ccgttttttc tttttcaatt catccgtatc aatgtgtaat tttctttgga
     7381 aataagactt cccagcttca gtgagttgga agtctttttc tgatcggatt gaagattact
     7441 ggactttcag taaacccagc tcgtagcttg gcaccagctg tcttggtggg cggcgcagcc
     7501 cttcaacaag tttggatttt catccttgca ccaatcgctg gtggagttct tgcagccctt
     7561 gttgcaaaaa atttccttgg aacagaagaa taattgaaac tcaaaaagcc ttgctcctca
     7621 tcttgaggaa cagggctttt tcgtatgata ctcttcgaaa atctcttcaa accacgtcag
     7681 cttcaccttg ccgtaggtat ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca
     7741 gtgttttgag taacctgcgg ctaacttcct agtttgctct ttgattttca ttgagtatga
     7801 ctttagcggt tgtcaatttt ctctggataa aggtcgtgtt ggaagaggcg ttgttctgcc
     7861 aagccctcat acttagttcc ttgcttaccg tagttgtagt aggggttgat tgaaatgcca
     7921 ccgcgcggag tgaatttccc ccagacttct aaatagcgag ggtctagcaa gttgaccaag
     7981 tctttcccga tggtgttgat acagttttcg tggaaatctc cgtggtttcg gtagctaaat
     8041 agatatagtt tgagggattt tgactcgaca cagagtttgt caggaatgta ggaaatatga
     8101 atcgtcgcaa agtctggctg agcagtgatt tgtcccagca gagacatatc gaggatatgg
     8161 tgacgaatgc cctgttcctt agcgatttct ctattaattt gaatttcgag gtgatgacgt
     8221 tggccgtagg caaaggtgac agcttcgact gtttcatagt gttgcatggc ccagaaaagg
     8281 caggttgttg aatcttgacc accactaaag acgaccaagg ctgattgacg tttcatagta
     8341 ctccttccaa aatgggaaat gttcagagca tgcaaaaagc tcccattagg gagctaaaaa
     8401 ataccaaatc gagttttttt agcgatggca tatcccaaac atcgtaatat tctacttata
     8461 tagtaaaa
//
LOCUS       DAVLXF010000081         8458 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLXF010000081 DAVLXF010000000
VERSION     DAVLXF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.081"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            194..1639
                     /gene="cps4A"
                     /locus_tag="VYO12_001728"
     CDS             194..1639
                     /gene="cps4A"
                     /locus_tag="VYO12_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1588284.1"
                     /translation="MSRRFKKSSSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     IDQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVLEIDDSSLAVVKAAIQDVMEGR"
     gene            1641..2372
                     /gene="cps4B"
                     /locus_tag="VYO12_001729"
     CDS             1641..2372
                     /gene="cps4B"
                     /locus_tag="VYO12_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV1588285.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRIIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHVIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            2381..3073
                     /gene="cpsC"
                     /locus_tag="VYO12_001730"
     CDS             2381..3073
                     /gene="cpsC"
                     /locus_tag="VYO12_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1588286.1"
                     /translation="MKEQNTIEIDVFQLFKTLWKRKLMILIVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRDQGDKPGLTNQDLQAGSYLVKDYREIILSQNVLEKVATNLKLD
                     MPTKTLASKVKVTVPTDTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELLDTRVKRPEDVEDVLQIP
                     LLGVVPDLNKMK"
     gene            3083..3772
                     /locus_tag="VYO12_001731"
     CDS             3083..3772
                     /locus_tag="VYO12_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV1588287.1"
                     /translation="MPTLEISQAKLDFVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     LGEGKSTTSTNIAWAFAHAGYKTLLIDGDIRNSVMLGVFKARDKITGLTEFLSGTTDL
                     SQGLCDTNVENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRKCDASILVTEVGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSVDKYGSY
                     GNYGDYGKNKK"
     gene            3788..5155
                     /locus_tag="VYO12_001732"
     CDS             3788..5155
                     /locus_tag="VYO12_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1588288.1"
                     /translation="MNGKIVKSSLAIIQSFLVILLTYLLSAVREAEIVSTTAIALYIL
                     HYFVFYISDYGQDFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNEVVGEL
                     VAVSVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIREMAGLNVVTFSTTFYKTSHVIAKRIIDIMGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGCFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVLM
                     KDGAK"
     gene            5186..6358
                     /locus_tag="VYO12_001733"
     CDS             5186..6358
                     /locus_tag="VYO12_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV1588289.1"
                     /translation="MKQSVYIIGSKGIPAKYGGFETFVEKLTEYQKDGNIQYYVACMR
                     ENSAKSGFTADTFEYNGAICYNIDVPNIGPARAIAYDIAAVNKAIELSKGNKDEAPIF
                     YILACRIGPFISGLKKKIRSIGGRLLVNPDGHEWLRAKWSLPVRKYWKFSEQLMVKHA
                     DLLVCDSKNIEKYIREDYKQYQPKTTYIAYGTDTTPSSLKSEDAKVRNWYREKGVSEN
                     GYYLVVGRFVPENNYETMIREFIKSKSNKDFVLITNVEQNKFYDQLLKETGFDKDLRV
                     KFVGTVYDQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     DGAIYWKKDELAHVIEEVERFDEGDITELDEKSSQRIADAFTWEKIVSDYEEVFTV"
     gene            6355..7638
                     /locus_tag="VYO12_001734"
     CDS             6355..7638
                     /locus_tag="VYO12_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1588290.1"
                     /translation="MKILHYTLGFQPSRTGGLVKYAEDLMTQQATDGHEVIALYPGEI
                     SIIFRQVRIKKAENRKFQCYKIINSLPLALFGGISEPDEFMKSCDKASYRKFLKNIKV
                     DVIHIHSLIGLHKEFIEVAKELGIKTVFTSHDYYGLAPVPHFYFNGVDYSSNNTNLAW
                     NIMSSNALSVKKLRFFQVSFYPTIRKLMKMLGKNPKSKKNLVIRDITEAIDYSALRSY
                     YKAMFGLIDAYLFNSQLAKEVYEANHIIPKNNIIVSISNSSISNHNIRVSSGEKTVVA
                     YIGPDEEYKGYFDFLDIVNDLDQEKYDIKTYGHFPNEDCPPYIQQNGYFTKDTVGSVY
                     SNIDILIVPSRWKETFGLISIEALSYDVNVFVSKNVGSKDLVSEEHIFHNKDDLLKKI
                     QYVKFENVKFKTMKEHMVEIINYYERITNDNQKIN"
     gene            7616..8305
                     /locus_tag="VYO12_001735"
     CDS             7616..8305
                     /locus_tag="VYO12_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV1588291.1"
                     /translation="MIIKRLIKTITGKEIDLHPDIPITYILQRGFQYGLGLSRGLLRG
                     IGLGKKGKRLFIGRGVTLLAKRKLYVGNNIRIGKYVSIDCLSKDGIQIADNVKIGDYS
                     QIIGTGSVKNMGIGLKIGKNSSFSEYSLFGSAGGITIGGDVIAGQNVRFHAENHSYEV
                     LHIPIREQGVNRKGISVGDNCWIGAGAVFLDGSSIGSGCIVATNAVVTKKFPDNVIIG
                     GVPAKILMERK"
     gene            8349..>8458
                     /locus_tag="VYO12_001736"
     CDS             8349..>8458
                     /locus_tag="VYO12_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEV1588292.1"
                     /translation="MKIAVVGTGYVGLSISVLLAQHHHVVAVDIVKEKVE"
BASE COUNT         2704 a         1341 c         1854 g         2559 t
ORIGIN      
        1 cttctaaaat attgttagaa aacgatttga ctgtcctgat caatttgtca tgttcttatt
       61 tcattttact atatttttgg ttcgcgggaa gtctactaag atacttaaag atgcagatag
      121 tgaaaataaa ggtgtagaca ttaccgtaaa aaagtgatat aatcgtatga tgttcaatgt
      181 ataggtgtta atcatgagta gacgttttaa aaaatcaagt tcacagaaag tgaagcgaag
      241 tgttaatata gttttgctga ctatttattt attgttagtt tgttttttat tattcttaat
      301 ctttaagtac aatatccttg cttttagata tcttaatcta gtggtaactg cgttagtcct
      361 actagttgcc ttggtagggc tactcttgat tatctataaa aaagctgaaa aatttactat
      421 ttttctgttg gtgttctcta tccttgtcag ctctgtgtcg ctctttgcag tacagcagtt
      481 tgttggactg accaatcgtt taaatgcgac ttctaattac tcagaatatt caatcagtgt
      541 cgctgtttta gcagatagtg agatcgaaaa tgttacgcaa ctgacgagtg tgacagcacc
      601 gactgggact gataatgaaa atattcaaaa actactagct gatatcaagt caagtcagaa
      661 taccgatttg acgatcgacc agagttcgtc ttacttggca gcttataaga gtttgattgc
      721 aggggagact aaggccattg tcttaaatag tgtctttgaa aatatcatcg agtcagagta
      781 tccagactat gcatcgaaga taaaaaagat ttataccaag ggattcacta aaaaagtaga
      841 agctcctaag acgtctaaga atcagtcttt caatatctat gttagtggaa ttgacaccta
      901 tggccctatt agttcggtgt cgcgatcaga tgtcaatatc ctgatgactg tcaatcgaga
      961 taccaagaaa atcctcttga ccacaacgcc acgtgatgcc tatgtaccaa tcgcagatgg
     1021 tggaaataat caaaaagata aattaaccca tgcgggcatt tatggagttg attcgtccat
     1081 tcacacctta gaaaatctct atggagtgga tatcaattac tatgtgcgat tgaacttcac
     1141 ttctttcttg aaaatgattg acttattggg aggggtagat gttcataatg atcaagagtt
     1201 ttcagctcta catgggaagt tccatttccc agtagggaat gtccatctag actctgagca
     1261 ggctctaggt tttgtacgtg aacgctactc actagccgat ggagaccgtg accgtggtcg
     1321 caaccaacaa aaggtgattg tggctatcct tcaaaaatta acgtcaaccg aagcactgaa
     1381 aaattatagt acgatcatta atagcttgca agattctatc caaacaaata tgccacttga
     1441 gactatgata aatttggtca atgctcagtt agaaagtgga gggaattata aagtaaattc
     1501 tcaagattta aaagggacag gtcggacgga tcttccttct tatgcaatgc cagacagtaa
     1561 cctctatgtg ttggaaatag atgatagtag tttagctgta gttaaagcag ctatacagga
     1621 tgtgatggag ggtagatgaa atgatagaca tccattcgca tatcgttttt gatgtagatg
     1681 acggtcccaa gtcaagagag gaaagcaagg ctctcttggc agaatcctac aggcaagggg
     1741 tacgaatcat tgtctctacc tctcaccgtc gcaagggcat gtttgaaact ccggaagaga
     1801 agatagcaga aaactttctt caggttcggg aaatagctaa ggaagtggcg agtgacttgg
     1861 tcattgctta cggagctgaa atttactaca caccagatgt tctggataag ctggaaaaaa
     1921 agcggattcc gaccctcaat gatagtcgtt atgccttgat agagtttagt atgaacactc
     1981 cttatcgcga tattcatagc gccttgagca agatcttgat gttgggaatt actccagtca
     2041 ttgcccacat tgagcgctat gatgctcttg aaaataatga aaaacgcgtt cgagaactga
     2101 tcgatatggg ctgttacacg caagtaaata gttcacatgt cctcaaaccc aaactttttg
     2161 gcgaacgtta taaattcatg aaaaaaagag ctcagtattt tttagagcag gatttggttc
     2221 atgtcattgc aagtgatatg cacaatctag acggtagacc tcctcatatg gcagaagcat
     2281 atgaccttgt tacccaaaaa tacggagaag cgaaggctca ggaacttttt atagacaatc
     2341 ctcgaaaaat tgtaatggat caactaattt aggagaaatg atgaaagaac aaaacacgat
     2401 agaaatcgat gtatttcaat tatttaaaac cttgtggaaa cgcaagctaa tgattttaat
     2461 agtggcactt gtgacaggtg cgggggcttt tgcatatagc acttttattg ttaagccaga
     2521 atatacgagt accacgcgaa tttacgtagt gaatcgcgat caaggagaca agccggggct
     2581 gacaaatcag gatttgcagg caggatctta tctggtaaaa gactaccgtg agattatcct
     2641 ttcgcagaat gtattggaaa aagtagcgac aaatttgaag ttggatatgc caacaaaaac
     2701 gttagccagc aaagttaaag tgactgtacc aactgacact cgtatcgtct caatctctgt
     2761 caaagataaa caaccagaag aagccagtcg tatcgctaat tctctacgag aagttgctgc
     2821 agaaaagatc gtcgctgtaa cgcgagtatc tgatgtaacg acacttgaag aagcgcgacc
     2881 agctacgact ccctcttctc caaatgttcg acgcaattcc ttgtttggtt ttcttggagg
     2941 agcagtcgta acagtaattg ctgttctttt gattgagttg ctcgacaccc gtgtgaaacg
     3001 tcctgaagat gtcgaagatg tactgcaaat tccactttta ggggtcgttc cagatttgaa
     3061 caaaatgaaa taggaggaag ttatgccaac attagaaatc tcacaggcaa aattggattt
     3121 tgtaaaaaag gcagaggaat attataacgc tttgtgcacg aacctacagt taagtggaga
     3181 tgatttgaaa gtattttcta tcacttctgt gaaactagga gaaggaaaat caacgacttc
     3241 caccaatatc gcttgggctt ttgcgcatgc aggttacaaa acgctgctga ttgatggaga
     3301 tattcgcaat tctgttatgt taggtgtctt taaagcaagg gataagatta caggcctgac
     3361 agaattttta tcaggaacta cagacctatc acaagggctt tgtgatacca atgtcgaaaa
     3421 tctctttgta attcaggctg gctctgtgtc accgaatccg acagctcttc ttcaaagtaa
     3481 gaatttcagt acaatgcttg aaaccttgcg taaatatttt gactacatca ttgtagatac
     3541 tgctcctgtc ggtgtcgtga ttgatgcggc tattattacg cgaaaatgcg atgcttctat
     3601 tttagtgacg gaggtaggtg aaataaatcg acgggatatt caaaaagcaa aagaacagtt
     3661 ggaacacaca ggaaagccgt ttttgggagt tgtgttgaat aaattcgata cttcagtaga
     3721 caaatacggt tcttatggaa attatggaga ttacgggaaa aataaaaaat aggtcggggg
     3781 attagagatg aatggaaaaa tagtaaagtc ttcattggcc ataatccaga gttttcttgt
     3841 tattttattg acttatctac ttagtgctgt gagagaagcg gagattgttt caacaacagc
     3901 tattgcactt tatatcctcc attattttgt cttttatatc agtgattatg gacaggattt
     3961 ctttaaaaga ggatatttga ttgaacttgt ccagacattg aaatatatcc tattctttgc
     4021 actagcgatt agtatttcta attttttctt agaggatcga tttagtattt ccagacgagg
     4081 catgatttac ttcctcacat tacatgctct cttagtctat gtgctaaacc tatttatcaa
     4141 gtggtattgg aagcgggctt atcccaactt taaaggaagt aagaagattc tcctacttac
     4201 agcaacttct cgtgtcgaaa aggtactgga tagattaata gaatcaaatg aggttgttgg
     4261 ggagttggta gccgtcagtg tcttagataa accagatttt cagcatgatt gtttaaaggt
     4321 agtagcagag ggggagatag taaactttgc gactcatgag gtggtcgatg aagtctttat
     4381 caatcttcca agtgaaaaat acaatattgg agagcttgtc tctcagtttg aaacgatggg
     4441 aattgatgta acagttaatc taaatgcttt tgatcgtagt ttggcacgta acaagcaaat
     4501 tcgtgagatg gcaggattaa acgttgtgac tttttctaca acattttata agactagtca
     4561 tgtaattgct aagcggatta ttgatatcat gggtgcattg gtagggctga tactatgtgg
     4621 tttagtcagt attgtactgg ttcctttgat tcgaaaggat gggggctctg ctatttttgc
     4681 tcagacgcgt ataggaaaaa atggtcgtca gttcactttt tataagtttc gctctatgtg
     4741 tgtagatgcc gaggcgaaaa aaagagaact catggaacaa aataccatgc agggtggaat
     4801 gtttaaggtg gacgatgatc ctcgtatcac gaaaattggt tgttttatac ggaagactag
     4861 cttggacgag ctaccacagt tttataatgt tctaaaggga gatatgagtt tggttggcac
     4921 acggccacca acagtggacg agtatgagca ctatacccca gaacaaaagc gtcgtctgag
     4981 ttttaaacct ggcataacag ggttatggca ggtcagcgga cgaagtgaga ttaagaattt
     5041 tgatgaagtt gtcaaattag atgtagccta tatagacgat tggacaattt ggaaagatat
     5101 tgaaatttta ttgaagacag ttaaagttgt attgatgaag gatggagcga agtgattttt
     5161 gcccttcggt cttgttagga gagatatgaa acagtcagtt tatatcattg gttcaaaggg
     5221 gattcctgcc aagtatggag gatttgaaac ctttgttgag aaattaacag aatatcaaaa
     5281 agatggtaac atccaatact atgttgcttg catgcgcgaa aattctgcaa agtcaggatt
     5341 tacagcagac acatttgagt acaacggtgc tatttgttac aacattgatg tgcctaatat
     5401 tggtcctgct agagccattg cttacgatat tgcagcggtc aataaggcta ttgaattgtc
     5461 taagggaaac aaggacgagg ctcccatttt ttacattcta gcttgtcgta tcggaccttt
     5521 tatttctgga cttaagaaaa aaattcgttc gatcggaggc cgtttgctgg taaatccaga
     5581 tggtcatgag tggcttcgag ctaaatggag cttgccagtt cggaagtatt ggaaattttc
     5641 ggaacagttg atggtcaaac atgcagattt attagtctgc gatagcaaaa atatcgaaaa
     5701 atatattcga gaggactata aacagtatca gcccaagacg acctatatcg cttatggtac
     5761 agatactacc ccttcaagtc tgaaatcaga agatgccaaa gttcgaaact ggtatcgtga
     5821 aaagggagta agcgaaaatg gctattatct agtggtggga cgattcgttc ccgaaaacaa
     5881 ctatgaaacc atgattcgtg aatttatcaa gtctaagtcc aataaggact ttgtccttat
     5941 tacaaatgtg gaacagaata aattttacga tcagttgctc aaggaaacag gtttcgacaa
     6001 ggatctaaga gtcaaatttg tgggaacagt ctatgaccaa gaattgctca agtatatccg
     6061 tgaaaatgct ttcgcctatt tccatggaca tgaagttggg ggtaccaatc catcgcttct
     6121 tgaagcttta gcatccacta aactcaactt gttgttggat gtcggcttta accgtgaagt
     6181 tggtgaagac ggagccattt attggaaaaa agatgagcta gcgcatgtaa tcgaagaagt
     6241 ggaacgattt gatgaaggtg atattacgga attagacgaa aagtctagcc aacgaattgc
     6301 ggatgcattt acgtgggaaa agattgtgtc agattatgag gaagtgttta ctgtatgaaa
     6361 attttgcatt atactttagg ctttcaacca tcacgtacag gtggtcttgt caagtatgct
     6421 gaggatttga tgactcaaca agctacagat ggacatgaag taatcgcatt atatccagga
     6481 gaaatctcaa ttatatttag gcaggttaga ataaaaaaag cagagaatcg aaaattccaa
     6541 tgctataaaa taataaacag cctacccttg gctctatttg gtggtatttc agaaccagat
     6601 gaatttatga agagttgtga taaagcttct tataggaaat ttttgaaaaa tattaaggta
     6661 gatgtaattc acattcactc gcttatagga ttacataagg aatttattga agttgcaaaa
     6721 gaattaggaa ttaaaactgt atttacaagc catgattatt atggtttagc gccagttccg
     6781 catttttatt ttaacggagt agattatagt agtaataata ctaatttagc atggaacatc
     6841 atgtcctcta atgctcttag tgtaaaaaaa cttcgattct ttcaagtctc tttttaccca
     6901 acaattcgga aattgatgaa aatgcttggg aaaaatccaa agtccaagaa aaacttagta
     6961 attcgtgata tcacggaggc tattgattat agtgctctcc gctcttatta taaagcaatg
     7021 tttgggttaa tagatgctta ccttttcaat agtcagttag ctaaggaagt ttatgaagct
     7081 aatcatataa tacctaaaaa caatatcata gtgtccattt ctaacagctc aataagtaat
     7141 cataatattc gtgttagttc cggtgagaaa acagtggtag cttatattgg gcctgatgag
     7201 gaatataagg ggtattttga ttttctagat atagttaatg atttagatca ggaaaagtat
     7261 gatattaaaa catatggaca ttttccaaat gaagattgtc caccttatat ccaacagaac
     7321 ggttatttca caaaagacac ggttggaagc gtttattcca atatagatat tttgattgtg
     7381 ccgagtaggt ggaaggaaac atttggtttg attagtatag aggcgctatc gtatgatgtg
     7441 aatgtttttg tgagcaaaaa tgtgggctct aaagatttgg tttcagaaga acatatcttt
     7501 cataataaag acgatttgtt aaaaaaaatt caatatgtaa aattcgagaa tgtaaaattc
     7561 aaaacgatga aagagcatat ggtggagatt ataaattact atgaaaggat tacaaatgat
     7621 aatcaaaaga ttaattaaga caattacagg taaagaaatt gatcttcacc ctgatatccc
     7681 aataacttac attcttcaga gaggttttca atacggtttg ggtcttagtc gagggttgtt
     7741 acgtggtatt ggtctaggaa aaaaaggcaa acgattattt attggacgtg gggtaaccct
     7801 tctcgcaaaa cgcaagttat atgttggaaa caatatccga atcggcaaat atgtgagcat
     7861 agattgcctt tcaaaggatg ggatccaaat tgcggataat gttaagatag gagattactc
     7921 gcaaataatt ggtacaggtt cagtaaaaaa tatgggtatt gggctcaaaa ttggaaagaa
     7981 ctcttctttc tcagaatata gtttattcgg ttctgcaggt gggattacta tcggaggaga
     8041 tgttattgct ggacaaaatg tacgttttca tgctgagaat catagctacg aagtgttgca
     8101 tataccaatc agagagcaag gtgttaatcg taaagggata tccgttggtg acaattgctg
     8161 gattggggca ggagctgttt ttttagatgg atctagtatc ggttcaggtt gtatcgttgc
     8221 aacgaatgcg gttgttacta agaaatttcc agataatgta attattggtg gagtgccagc
     8281 taaaatttta atggaaagaa agtagtatta aattaattgt ataaatggag ggaaatgata
     8341 tgtctaaaat gaagattgct gtagttggga caggttatgt tggtttatcc atttctgtgt
     8401 tacttgcaca acaccatcat gtagtagcag tagatattgt aaaagagaaa gtagaatt
//
LOCUS       DAVLXF010000082         8078 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLXF010000082 DAVLXF010000000
VERSION     DAVLXF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.082"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(94..679)
                     /locus_tag="VYO12_001737"
                     /pseudo
     CDS             complement(94..679)
                     /locus_tag="VYO12_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(655..831)
                     /locus_tag="VYO12_001738"
     CDS             complement(655..831)
                     /locus_tag="VYO12_001738"
                     /inference="COORDINATES: protein motif:HMM:NF015247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588293.1"
                     /translation="MQRNSGGKPTTELPSFIIRCLPVHLTYDNNYFNDTYQGIPIGGY
                     TQIVEKCWIMKILM"
     gene            complement(797..1267)
                     /locus_tag="VYO12_001739"
     CDS             complement(797..1267)
                     /locus_tag="VYO12_001739"
                     /inference="COORDINATES: protein motif:HMM:NF013739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEV1588294.1"
                     /translation="MYDYLVVGAVFAHEAALEGKKVKVIEKRNHIAGNIYTREEEGIQ
                     VHQYGAHIFHTSDKEIWDYVNQFAEFNRYTNSPVANYKGEIYNLPFNMNTFNKLWGVV
                     TPAEVQAKIDEQRAILNGKTPENLEEQAISLVGTDIYEKLIKDYTEKQWGQTNY"
     gene            complement(1330..2181)
                     /gene="rfbD"
                     /locus_tag="VYO12_001740"
     CDS             complement(1330..2181)
                     /gene="rfbD"
                     /locus_tag="VYO12_001740"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1588295.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            complement(2247..3296)
                     /gene="rfbB"
                     /locus_tag="VYO12_001741"
     CDS             complement(2247..3296)
                     /gene="rfbB"
                     /locus_tag="VYO12_001741"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1588296.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPGVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(3309..3902)
                     /locus_tag="VYO12_001742"
     CDS             complement(3309..3902)
                     /locus_tag="VYO12_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV1588297.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDL"
     gene            complement(3903..4772)
                     /gene="rfbA"
                     /locus_tag="VYO12_001743"
     CDS             complement(3903..4772)
                     /gene="rfbA"
                     /locus_tag="VYO12_001743"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV1588298.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLPRFKELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLTLAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(4799..5899)
                     /gene="glf"
                     /locus_tag="VYO12_001744"
     CDS             complement(4799..5899)
                     /gene="glf"
                     /locus_tag="VYO12_001744"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEV1588299.1"
                     /translation="MYDYLIVGAGLSGAIFAHEATKRGKKVKVIDKRDHIGGNIYCEN
                     VEGINVHKYGAHIFHTSNKKVWDYVNQFAEFNNYINSPVANYKGSLYNLPFNMNTFYA
                     MWGTKTPQEVKDKIAEQTAGMKDVEPKNLEEQAIKLIGPDIYEKLIKGYTEKQWGRSA
                     IELPPFIIKRLPVRLTFDNNYFNDRYQGIPIGGYNVIIENMLKDVEVELGVDFFAHRE
                     ELEASANKVVFTGMIDQYFDYKHGELEYRSLRFEHEILDEENYQGNAVVNYTEREIPY
                     TRIIEHKHFEYGMQPKTVITHEYPADWKRGDEPYYPINDEKNNAMFAKYQEEAAQNDK
                     VIFCGRLADYKYYDMHVVIERALEVVEKEFSN"
     gene            complement(5902..7344)
                     /locus_tag="VYO12_001745"
     CDS             complement(5902..7344)
                     /locus_tag="VYO12_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEV1588300.1"
                     /translation="MSTRNSLIKNSLYNVAYRMLNIIFPLVSATYTARIILASGVGEV
                     SFAQNVVSYFTTIAAMGIPNYGIREIAKTRNKAEATNKVFSELFLINGLSTLLCSIVY
                     LILILTFNSIFDNTSMYLAVGLTLVFNFLNIDWFYQGKEDYAYITKRSFSIKLISLFS
                     LFIFVRDTDDAVNYALISSLAIGGNNIFNLVNLRKYNIRFSFNNIKIRRHLKPIFVLL
                     GSVIAIEIYTMLDTTMIGAMVGTTEVGYYTNSMKLVKLLITVITAIGGVLLPRLSDYY
                     SKGEFEKLNHIVDKVFRIMLFLFLPAQVALILVAPVIMPILFGDSFIPAVLTLQLTSL
                     LICTLGFSNLFGTQILLTFGDERKLLISTILGALSNIALNLILIPVMAQNGAALASVI
                     SETIVTLTAYYYAKKRVTIRFDLGFYLPTVVSTIIMGGAIYVVMQLYIGDIEKMLFST
                     LLGGVVFLLINVFMKNPIIYEFTGIIKRKR"
     gene            complement(7349..>8078)
                     /locus_tag="VYO12_001746"
     CDS             complement(7349..>8078)
                     /locus_tag="VYO12_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588301.1"
                     /translation="SIQKAVWWMSVDNYLKRNGILGSLEHFGLLRTIKLFVKGNIKIG
                     GYRIDKEIPHLYQSEYAKQFLLAKGVKKYYRLSDYLNESYIQRSISTDTKKDVILYNP
                     KKGIEFTRELIKQAPELTFSPIENMSTEQVKKLLSKSKVYIDFGNHPGKDRFPREAAI
                     SGCCVITGKRGSAKYYKDIPIPDSYKFEDTVENIPNIIETLKSCLKNYDKHTVDFEKY
                     QQYIKSEHDLFERDVKELFTRNRG"
BASE COUNT         2435 a         1715 c         1301 g         2627 t
ORIGIN      
        1 catatagtca aggaattttt aatactattt ctaaataata actggtagtc agttttactt
       61 gtttatactc ttcgaaaatc tcttcaaacc gcgtcagctt ccatctgcaa cctcaaaaca
      121 gtgttttgag caacctgcgg ctagcttcct agtttgctct ttaattttca ttgagtatga
      181 atctaactca tttctcacgc actgcaaagc tgctccaatt acttggtgca tatcgtaata
      241 acgatagtgt cctaagcggc caccaaagat aacattccct tgctcatcag caagtttttt
      301 atatgattta tacaaatgat tattacgatc attattaact ggataataag gctcatcacc
      361 tttttcccat gttttagaat gttctattag taatgatagt ctttgcttga ctcccaaact
      421 caaaatgttt gtgttcaata atgcgagtat atggggtttc tgcatccgta tagttcacaa
      481 ctgcatttcc ttggtaattt tccatatcca aggtctcatt ttcaaaacga agactacggt
      541 actctagttc gcccaactta tagtcaaaga attcatcaat cataccagta aagacaatct
      601 taggaaaatc tttcagatat tgctctttgt tcacaaagaa atcaacattt gtttctacat
      661 caatattttc atgatccaac atttttcaac tatttgagtg tatccaccaa ttggaatccc
      721 ttgataggta tcgttaaaat agttgttatc ataggtcagg tgtactggta aacagcgaat
      781 aataaaggat ggaagttcag tagttggttt gcccccactg tttctctgta tagtctttga
      841 ttaatttttc gtagatgtct gtacctacaa gagaaatcgc ctgttcttcc aaattttcag
      901 gagttttacc atttaaaata gcacgttgtt catcaatctt agcctgtact tctgctggcg
      961 ttacaactcc ccagagttta ttgaaggtat tcatattaaa aggaaggtta taaatctcac
     1021 ccttatagtt tgcaacagga gaattggtat aacggttaaa ctctgcaaac tgatttacat
     1081 aatcccagat ctccttatca gaagtatgga agatatgagc accatattga tgaacttgaa
     1141 ttccttcctc ttcacgagta tagatattac ccgcgatatg atttcgtttt tcaataactt
     1201 ttactttttt tccttctaag gctgcttcat gggcaaagac tgcaccaaca acaagataat
     1261 cgtacatctt aagctccttt ggagttcatt atctgcctct tttattagac tagaaaatca
     1321 ttctactact tatctcactt cttgtttgta aaattcttgc aatgcatctt gccaagttgg
     1381 aataacaaat ccagtagctt tggctttggc caggctcatc gttgagttta gcggacgttt
     1441 agctttggct ggaaattgac tggaatctac tggcttgact tcgacatctg tatctttcaa
     1501 aatttcaact gcaaaatcat accatgttgt gtcttctgtc gcatcatttg acaaatgata
     1561 ataaccaaat tccttacgat tttcagctag gtaggtcatg aactcagcca aggtacgagt
     1621 ccaagtcgga cgaccgtact ggtcatttac aactgttaaa gtcttatgag ttttcgcaag
     1681 attttgcatg gtaaaaacga agtttttgcc ataatttcca aatacccagg cagtacggat
     1741 aatatagaaa ttagacacat gcttctcaac taactcttcc cccatacgct tagtgcgtcc
     1801 atattctgtc tgtggatctg gtcggtcatc aacttcccac tcttgtccaa ctggtttctt
     1861 accgtcaaag acatagtccg tagaaatata aactagagtt gcaccatgct tttcagatgc
     1921 ttttgcgaca ttttttgtcc ccgtcacatt gatggcgaag tccaactctt ttccttcatc
     1981 ctctgctgca tcaacagcgg tgtaggctgc acagtggtag actaaagtcg gtttcacctc
     2041 ttcaaaaact ttctcaacca tttcttcatt ggtaatgtcc atctcagcca catctactgc
     2101 cacgtattct tcattacgtt catccaataa atagcgaagt tccgttccta attggccatt
     2161 tgcccctgta attaaaatca tctctagacc tttctaatat gtaactcttc tcaatatagc
     2221 aataaaaagc agctatttcc tgctttttat actgtaataa tctcctgagt cttagcataa
     2281 ttggcttcaa cagcttcttt ttctgctttc caccattctt ggttatctgt ataccacttg
     2341 attgttgcct tgagcccagc ttcaaagttg gtaaattcag gtttccaccc caactcatca
     2401 cggagcttgc tggcatcaat cgcatagcga aggtcatgtc ctgcacggtc agtcacatga
     2461 tcataggcat ccgtagcttg tcccatttcc ttaaggataa gttccaaaac ttccttattg
     2521 ttcttctcac catcagcccc aatcaagtag gtttcaccga tttgcccttt tgtcaagatt
     2581 gtccaaactc ctgaagaatg gtcattggta tgaatccagt cacgaacatt cttaccttca
     2641 ccgtaaagtt ttggcttaat tccacttagg atgttagtaa tctgacgtgg gatgaatttt
     2701 tcgatatgtt gataaggacc gtagttattt gaacagttgg aaatcgttgc cttgactcca
     2761 aaagaacgca cccaggcttt gacaatcaaa tctgaggctg ccttggttga tgagtacgga
     2821 gagcttgggt tgtattttgt ttcagcagta aatttctcac ctggtccttc accatgacct
     2881 ggcaaatctt cgcgtaaagg gagatcccca taaacttcat ctgtcgatac atggtggaag
     2941 cgaatatcat acttacgagc agcttctaaa agagtatagg ttccgatgaa gttagtatga
     3001 ataaatggcg atggatcatt gagcgaatta tcattgtggc tttccgctgc ataatgaacg
     3061 atagcatctg cttgagcagc caacttgtct accaactccg catcagcaat gtcaccaaca
     3121 actaactcaa cacgattacc taaaatttcc tcaatattcg cgcgatttcc agcataagtc
     3181 aacttatcta ggactgtcac gtgaacacct ggaaagttct cgtaaacata atggacaaag
     3241 ttagaaccga taaagccagc tccacctgtc acgataatat ttttgtattc agtcatattc
     3301 tttccttttt acaaatcttc ttttttcaaa ggttttacat ccttaagtag gggatgatgt
     3361 ttatctgctt ctgaaacctc tgcttctgca atattttccc attcaatacc aaggcttgga
     3421 tcagcgtagt tcacaaaggc atacttgggt ttgagctcga gagcccagta atcattgacc
     3481 agatagctat atgacactgt atctgataga acttggaagc cattagctac gcctcgagga
     3541 acaaagattc ccttgcttgc atcaattact gtctgatagg tattcccaaa ggtttcaccc
     3601 tcgcgtagat caacccaaga acccagaact ttccctccat ctgctacaga gatgtactta
     3661 tcccaaggct ctgcgtggag tcctcgaagg acatttttac gtgagaagga tacattgttt
     3721 tgcaattttc cttctgcaaa gaaagactct ggaaatccaa gtggaagcat tttttccttt
     3781 tggaaatttt ctttaaacca gccacgatta tctccatgaa cggggatatc aaactccaac
     3841 atgcctggaa tagcttcaac cttgcgtgcc gcaagcgtct taccgaaaaa attatctgtc
     3901 atctatgctt ctccaatcaa acggagcaga tactgtccgt attcattttt cttaagtggt
     3961 tgggctaagg tcaatacatc ttcacgactg atataaccca tgcgataggc aatttcttct
     4021 aagtttgcta cctgaacatt ttgcatccgt tggactgttt cgatgtactg tgaagcctct
     4081 agtaaacttt catgagttcc agtatccagc caagcaaagc cacgtcccat aagctcaaca
     4141 gacaaatcac cacgatctag gtaagctttg tttacatctg taatttccag ttcaccacga
     4201 ggacttggtt taatactctt ggcaatctct actacatcat tatcatagaa atagagacct
     4261 gtaactgcat agtttgaacg aggttgctct ggcttttctt caatagaaat agccttcata
     4321 tcctgatcaa actcaaccac accaaagcgc tctggatcct tgacatggta gccaaaaaca
     4381 gttgctcccg actccttact cgctgccttt tgtagcatct tggaaagccc agaaccatgg
     4441 tagatattat cacctaagat tagcgcaacg ctatcatcag aaataaactc ttccccaatg
     4501 ataaaggctt gtgccaaacc atctggactt ggttgctcag cataagaaag tttaatacca
     4561 aactcggaac catcttgaag aagctcttta aaacgaggca aatcttgagg agttgagata
     4621 atcaaaatat ccctaatccc agccaacatt aatgttgaaa gtgggtagta aatcatcggt
     4681 ttatcataaa ccggcataag ttgttttgat gcggctcgag tcaaaggata caagcgagtt
     4741 cccgaaccac ctgctagaat aatacctttc ataataagca acctttcttc gttgtttttc
     4801 aattactaaa ttctttctcc acaacctcaa gcgcccgttc aatcaccaca tgcatatcgt
     4861 aatacttata atctgctaaa cgcccacaaa agataacttt atcattctgc gctgcttctt
     4921 cttggtattt agcaaacata gcattatttt tctcatcgtt aataggatag tagggctcgt
     4981 ccccacgctt ccagtcagct ggatattcat gcgtgataac tgtctttggc tgcattccat
     5041 attcaaaatg tttgtgttcg atgatacgag tataagggat ctcacgctct gtatagttca
     5101 ctacagcatt cccttgataa ttttcctcgt ctaaaatctc atgctcaaaa cgaaggctac
     5161 ggtattctaa ctctccgtgc ttgtagtcaa aatattggtc gatcattcct gtgaagacaa
     5221 ctttgttagc agatgcttcc aactcttcac gatgagcaaa aaagtctact ccaagttcaa
     5281 cttctacatc tttcagcata ttttcgatga tgacattgta accaccaata ggaatccctt
     5341 ggtaacggtc attaaaatag ttattatcaa aggttaaacg aactggaagg cgttttatga
     5401 taaatggagg aagttctatc gctgagcgtc cccattgctt ttctgtataa cccttgatca
     5461 acttttcata gatatccgga ccaatcaact tgatagcttg ttcttccaga tttttcggct
     5521 caacatcctt cataccagct gtctgctcag caatcttatc cttcacttct tgtggagttt
     5581 ttgtgcccca catagcatag aaggtattca tattgaaagg aaggttataa agacttccct
     5641 tatagtttgc gacaggtgag ttgatatagt tgttaaactc agcaaattga ttgacgtagt
     5701 cccagacttt tttattagaa gtatggaaga tatgggcacc atatttatga acattgatac
     5761 cttctacatt ctcacagtat atgttccctc caatgtgatc gcgtttatca atcactttta
     5821 ctttttttcc acgctttgta gcttcgtgtg cgaagattgc tccagacaaa cccgctccaa
     5881 cgattaaata gtcgtacata attatctctt tctctttata atacctgtaa attcatatat
     5941 aataggattc ttcataaaaa cgttaattaa aaggaataca acacctccta acaaagttga
     6001 aaataacatt ttttctatat ccccaatata caattgcatt actacatata tagctccacc
     6061 catgattatt gttgaaacaa cagttggcaa ataaaatcct aagtcaaacc taattgtcac
     6121 acgttttttc gcatagtagt atgctgttaa agtaacaatt gtttcactaa tcacagatgc
     6181 caacgctgct ccattttgag ccattactgg aatcagaatt aggttcaaag cgatattact
     6241 aagcgctcct aaaattgtgc taatcaaaag ctttcgctca tctccaaatg ttagtaatat
     6301 ttgagtccca aataaattac taaaccctaa tgtacatata agcaaagaag taagttgaag
     6361 tgttaacact gctggtataa aactatcacc aaataaaatc ggcataatta ctggtgcaac
     6421 tagaattaat gcaacttggg ctggtaaaaa aagaaacaac atgattctaa aaactttatc
     6481 aacgatgtga tttaattttt caaattctcc cttagaataa taatcactta gtcgtggtaa
     6541 aagcactcct ccaatagcgg taatcacagt tatcagcaat ttaactagct tcatagaatt
     6601 tgtataataa cctacctcag tcgttcctac catagcacca atcatagtgg tatccagcat
     6661 agtatagatt tcaatagcaa tcactgaacc caacaaaaca aaaataggtt ttaagtgtct
     6721 tctaatctta atattattaa aactaaatct aatattatat ttccttaaat taacaagatt
     6781 aaaaatatta ttcccgccaa ttgctaagct agaaatcaat gcatagttaa ctgcatcgtc
     6841 agtatctcta acaaaaataa ataaactaaa aagagaaata agttttatac tgaaactacg
     6901 tttcgtgatg tatgcatagt cctcttttcc ttgatagaac caatcgatat taaggaagtt
     6961 aaacactaat gttaaaccca cagcaagata cattgatgta ttatcaaaaa tgctattgaa
     7021 tgtcaaaatc agaattaaat atactataga acacagtaat gtagacaatc cattaatcag
     7081 aaatagctct gaaaaaactt tattcgtagc ctctgcttta tttctagttt tagcaatttc
     7141 tcgtatacca taatttggaa tacccatagc agctatcgtt gtaaagtaag aaacaacatt
     7201 ttgtgcaaac gagacctccc caactccact agctaaaatt atccgtgcag tatatgttgc
     7261 ggatactaat ggaaatatga tatttaacat tctataagca acattataaa gagaattttt
     7321 aataagtgaa tttctggttg acataacctt aacccctatt cctagtgaat aattctttca
     7381 catctctttc aaataaatca tgttcagatt taatatattg ttgatatttc tcaaagtcaa
     7441 cagtatgttt gtcataattt tttaagcatg atttcagggt ctcaataatg ttggggatat
     7501 tttcaacagt gtcttcaaat ttgtatgaat ctggaatagg aatatcctta taatattttg
     7561 cagaacctcg tttcccagtt atcacacaac aacctgatat cgctgcctcc cgtgggaatc
     7621 tatctttccc aggatggttg ccaaaatcaa tatatacttt gctttttgaa agtaattttt
     7681 tcacctgttc agttgacata ttctcaattg gagagaacgt taattctgga gcctgtttaa
     7741 ttagctcacg agtaaattca atcccttttt taggattgta aagaataaca tcttttttag
     7801 tgtctgttga aatagatcgc tgaatataag actcgtttaa ataatcagac aatctatagt
     7861 actttttgac acctttagct agaagaaatt gcttggcgta ttcactttga taaagatggg
     7921 gaatctcttt atctattcta tatccaccta ttttaatatt tccttttaca aataatttaa
     7981 ttgttcgtaa aagtccaaaa tgttccaagg aacctaaaat accattacgc ttcaaataat
     8041 tgtcaacgct catccaccat actgcttttt gaatagac
//
LOCUS       DAVLXF010000083         8022 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLXF010000083 DAVLXF010000000
VERSION     DAVLXF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.083"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            133..825
                     /locus_tag="VYO12_001747"
     CDS             133..825
                     /locus_tag="VYO12_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1588302.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(928..2573)
                     /locus_tag="VYO12_001748"
                     /pseudo
     CDS             complement(928..2573)
                     /locus_tag="VYO12_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(2563..3654)
                     /locus_tag="VYO12_001749"
     CDS             complement(2563..3654)
                     /locus_tag="VYO12_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588303.1"
                     /translation="MSEIEIINAKKIYHDVSVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNLSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(3667..4788)
                     /locus_tag="VYO12_001750"
                     /pseudo
     CDS             complement(3667..4788)
                     /locus_tag="VYO12_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(5006..5203)
                     /locus_tag="VYO12_001751"
     CDS             complement(5006..5203)
                     /locus_tag="VYO12_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1588304.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(5278..6054)
                     /locus_tag="VYO12_001752"
     CDS             complement(5278..6054)
                     /locus_tag="VYO12_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1588305.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            6176..6922
                     /locus_tag="VYO12_001753"
     CDS             6176..6922
                     /locus_tag="VYO12_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1588306.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            6935..7915
                     /locus_tag="VYO12_001754"
     CDS             6935..7915
                     /locus_tag="VYO12_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1588307.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT         2578 a         1236 c         1619 g         2589 t
ORIGIN      
        1 ttgattttca tctaatatct ttatttaatg aactcctaaa ctttttcata ataatctcct
       61 tcaaaagtcg cctgtatggg tggcttttat tttatcattc atgatataat agaagcaaac
      121 ggaggacgga aaatggtaaa agtacgattg tatttggtac gtcatggcaa gaccatgttt
      181 aacacgattg gtcgcgcgca aggttggagc gatactccct taactgctga aggtgaacga
      241 gggattcaag agttaggaat cggtttgcga gaatctgatc tacagtttga gcgcgcttat
      301 tcgagtgatt ctggtcgtac cattcagacc atgggaatta tccttgaaga acttggcttg
      361 caggggaaaa tcccttatcg catggacaag cgtatcagag aatggtgttt cggtagtttt
      421 gatggagcct atgatggcga tcttttcatg ggcattattc ctcgtatctt taatgtggac
      481 cacgttcacc aattgtctta tgctgaactg gctgagggct tggtagaggt cgatacagct
      541 ggttgggctg aaggctggga aaaactcagt ggccgaatca aggaaggctt tgaaatgatt
      601 gcaaaagaaa tggaagatca aggtggaggt aacgcccttg ttgtcagcca tggaatgact
      661 attggaacca ttgtttatct gattaatggc atgcatccgc atggtctaga taatggtagc
      721 gtgacgattc ttgaatatga ggacggtcag tttagggttg aagttgtcgg tgaccgtagt
      781 taccgagagc taggacgtga gaagatggaa gaaggctcta tttaatcagt ctagacttgc
      841 ttgccatgag ctagggattt gataagaata tcaagataag aaaaaacagc cgagggtact
      901 cctttcggct gtttttgatg tggaaaacta aagtgtaatg ctattgcttt tagagatttt
      961 cataaacaag agcaaggaac ctactgttag aacagtcagg atagttgaca aggctgcggc
     1021 tacaccgtaa tttcctctga gaacctctgt ataaatagct acagtcattg ttcttgtttt
     1081 gacattgtag aggaggatag aagtagagag ttttgaaatc attgtgaccc aagataagat
     1141 ggctccagaa atgataccag atagcatcat tggagttgta atcttagcaa aggtattgag
     1201 acgactactt cctaagcttt cagcagcttc ttcaatactt ggtgctattt gttgtaagct
     1261 agcaacagat gagcgaatag tataaggtaa tcttctgaca gatagagaca taatcaagat
     1321 gaaagcagtc cctgtaatca taagaaatcc acttccaaat agaccagtat tgaaggaaga
     1381 aatgaaggca atccctagaa cggttcctgg tacaatataa ggtaccatac tgaggctgtc
     1441 aattaagttt gtaaacaaat tccgttttct aacggctagg taggagataa atgtcgcaaa
     1501 tagaacaact agaactaagg caatcaaagg gatacgaatg gtattgaaaa tagcagatcc
     1561 catacgatgg aaagctacct tgtaactgtt tggagaataa cctttaacag ataccatacc
     1621 tgatgttttt aggaaagagg tataaattaa gtagatttga ggtaaaacag agataaagat
     1681 aattccgtag actgttgcat aaatggcagc tatttttcct tttgtagttt ttttaggctc
     1741 aattggatgg agcagattca tgctgaaact gtagcggttt gcaatgtgtt tttggataag
     1801 gaaaatcgcc aaggcaatga taatcgccat aattgcaaaa gcagaatttc ctccaacctc
     1861 gctaataaat tgggtataaa tcaggacagg gaaagtccga tatccttcgc caatcaacat
     1921 aggcgttcca aagtctgaga atgctctcat aaatacaagc aaggaactgc tagtaaggtt
     1981 ggaactagga gaggtaaaac aaccgttacg atacgcttaa atccgaagga ccccatgctt
     2041 tcagctgctt caagtagaga attgtcaata ctgttcattg ttccagcaac atatagaaat
     2101 accagtggga atagttgcag tgtaaagaca agtacaattc ctttgaatcc ataaatatcg
     2161 atagctggaa gatgaagggc atttgtcaaa aatttagtga tgaccccatt tcgtcctagc
     2221 aagagaaccc aggagtaggc tcctacgaaa ggagctgaca tggaagcaat gataatcaat
     2281 atttgtagaa atttctcccc cttgaagtca tacatagaga agagataagc taatagggtt
     2341 cctacaacta aggaagtgac agtagcggta atggaaacct tgaaactgtt gactagtgtc
     2401 tcagagtagt agactttact aaagaaagtg acaaaattag ctagtgagaa ttgtccttca
     2461 tgtataagtg cttacttgag cacggtaacg ataggataaa cgagaaagac aagataggta
     2521 agaaagatga agaaagagga ggctgtccaa atatttagtt ttttacgttc catggttgac
     2581 tccttttatc aggttttggg aaccatctgc agaaaagatg tttaattttt gcgtattgat
     2641 tcgtagacga atacgattgc ctttttgtag atcttcttca aaagttgatt cttcactaac
     2701 ttgaattttt gaggcaaaac ctgtctcaat gaaatactcc gtatttagtc caagatagac
     2761 gctatcgcta atagttcctt caatatctcc agattcatct ttgataaact cttcgggacg
     2821 aatgcttaca tgaatagctt gctcctcaac ctgatcaaga gctagcattc gaagggcata
     2881 gccatctgaa aagacgatat aagcgccgtc gctccgtttt tcaagattgg cagggataat
     2941 atttgtgcgt ccgataaagg ttgccacaaa ctcattagct ggtttatgat agagttcttt
     3001 tggtcggccg atttgttgga tcaccccatc tttcataaca gcaatttggt ctgaaatagc
     3061 catggcttct tcttggtcgt gggttacata aacagttgta attcccactt cgtgttggat
     3121 ttctcggatg gcttgacgca tatccaagcg aagtttggcc tccagattac taagtggctc
     3181 gtccatgagg agaacactta gattaaccgc taaggcgcat gccaaggtga cacgttgttg
     3241 ttgtccacca ctgagtttat cgggctttcg atccgcatat tgagcaattt gcatgagttc
     3301 aagatacttg ttggtctgtt gaatcaattc ttcttttgga accttctttt gcataagacc
     3361 aaaagcaacg ttgtctcgga cagtcaaatg tgggaaaata gcgtagtttt ggaaaaccat
     3421 cccgatattg cgtttgctgg gttccatatt attgattttt gtatcatcga agtaaaattc
     3481 tccatctttg atactgttga aacctgcaat catacgaaga agggtcgttt tcccacatcc
     3541 tgaagctcca agaagggtaa agagacttcc ttttggaatt gtaatgttca aattctcaat
     3601 aacagagaca tcgtggtaga tttttttggc gttaataatt tcgatctcac tcatagtgaa
     3661 cctcttttac tgtttagatt ggatatctgt aaagacttcg ttgtatttct taacgatatc
     3721 tgatttattc ttgatgacat aatcataatc ttcagtgagt gttttgattt tgtcaattgg
     3781 tttcatgttt tcgcttgttt tagcattttt acgaacagga cggttagtag tggttgtacc
     3841 aagtgtatct tgtacttctt gagagataat aaaatcgata aatttcttgg cattttccat
     3901 atttttagat tttttaacga tagcagcact agcaggtagg aagacggttc cttcttttgg
     3961 atagactacc ttaatgttag ctccgtcatt taagagttta actgctggat cttcataaga
     4021 gagaccaaca gtcatttctc catcagcgac tgctttatag acaccagatg aacttaaacc
     4081 gattttatag tatattgaaa ctagaatagt acacatctac ttctaaaaca ttgttagaaa
     4141 tcgatttgac tgtcctgatt aatttgtcct gttcttattt cattttacta taccatcaat
     4201 aagtgtgaaa agatctttta cataagacca agccttatca tctttgtaac caccttgagc
     4261 ttgtagcata tttgttaatt gagcaaaggc gctagaagag tttgctgggt cagcagttgc
     4321 gatttttcct tttagttcag gtttgagaag gtcgctatat ccttcgatgt tcatgccttt
     4381 agttaaatca gggtggacga ttaaaacact accatctagt gtataaggag tagagaagcc
     4441 agttgtgttt tgatattctt tgataacatt atcattttct tttgaagtat agttttcaaa
     4501 gagttctccg tgggtagcat attgtgtata agaaccacca aagataacat cagctacagg
     4561 aacttctttt tctgactcta gttttttgaa aagttctcca gtaccagctt gaatcagttc
     4621 tactttgata ccatattttt cttcaaaggc aggaatagtt gctccaatta agtcctctga
     4681 gtttggtcaa taaacgacta gcgaaccacc gtctccttta tcagatgaac tgtcatcggc
     4741 agattcatta gaagaacaag cagcataata catccatttc tttttcatga tgggatatct
     4801 ccgttgtgtt atttaagttt attttaaaac aatgtaagcg tttttaaaac ctacaattct
     4861 attctatagt gtattgaatc tataacagta cactttgact gctaaaatat ttctataaat
     4921 taatttgact ttcctgatag agatgttcac atcttatttc aattcactat attagagtaa
     4981 aattctctac aaaaagaaga atagcctatt ttactattct tccgagtgat ttcaatttct
     5041 ttggggaaat atggagatac tttttaaata ctttggcaaa atatttatag tctgaaaatc
     5101 caacttggtc agagatagcg cttaaagggg agttgggaga ttcagccatc ttatctatag
     5161 cttgtttcag gcgatattgt aagatatatt catttagagt catggggaag tcttctttga
     5221 taaccttata tagatgcttg ttatctgcaa atagctagag catttcaagg aaatgaacta
     5281 gaaataacaa ttaatatggt ggttcagaaa taccttttga taatcaataa gatcttcagg
     5341 atgaagtgcg ttgactccat ccatttcata tttccgattt aataaacgat atttgttttc
     5401 accaaattga tgaaaaggga gtagttgaac ttggtcgata tttaatgagt taaatagagt
     5461 agcgaatttt tctgcatcct ctaaactatt gttaaaatta ggaataactg ggattcttaa
     5521 aacgatagtt ttattttgag aaaaagcata atgaatgttt ttaataatca tttgattaaa
     5581 aaccccagtc acttttttat gttttataga attataatgt tttaggtctg tgtagataaa
     5641 atccacatat tgaattaaat cgataaattt ttcatgatca acaaaggcag tagtttcaat
     5701 ggcagtgtgt atgtgatgtt ctttagctga ttttaagatg gctttagcaa attcaaactg
     5761 agcaaatatt tcacctcctg ataaagttaa acctccaccg gattcttcgt aaaattcttt
     5821 gtcttttaat acctctgtaa taatttcttc tacagtcttt tcttcaccga ctaaggtgaa
     5881 tttctctcgt tgagcatctt tcattttttc aggtttcatt ctttgagatt caggattaga
     5941 acaccatgga cagcgcagag gacatccttt taaaaaaaca gttgtacgaa tacccgggcc
     6001 gtcatgaatt gaaaagtgtt gaatattaaa aataattcct ttagatattt ccataagagt
     6061 tcctcctctt cataagtcta tcctatcaca aaaacgaaag aaaaacaatt acgaacgaaa
     6121 ggttgatttg ttgttttttg ctttttattt tggtagaata aaacggaggt gtaaaatgaa
     6181 aagattggaa caaattatta aattagtatc agaatatgaa aagatcgacg ttaatacatt
     6241 atcggaaaaa ttaaatgtat cgaaagtaac gattagaaaa gatttagata aattagagtc
     6301 aaaaggttta ttacacagag agcatggata tgctgtatta aatagtggag atgacttaaa
     6361 tgtacgtttg tcaattaatt atgaaattaa gagaaaaatt gttcaggaag cagtaaaatt
     6421 ggtgtcagat aatgaaacaa taatgataga atctggatcg acctgtgctt tacttgctga
     6481 ggaaatttgc aagcaaaaaa gaaatgttac gattgtaaca aattcatttt ttatagcaaa
     6541 ttttgtgaga gcttatgatt catgtcgtat tattgttctt ggtggagagt ttcagaagga
     6601 ttcacaggtt actgtgggac ctttattaaa agaaatgata cagacttttc atgtgcatca
     6661 agcttttgtt gggacagatg gttacgataa agagatgggc tttacaggaa aagatttaat
     6721 gcgcagtgag gtagttcaat atatttcagc agcgtcggat aaagtcattg tactaactga
     6781 ctcaagtaaa tttgataaaa ggggtacagt aagaagattt gctttaagtc aagtctatga
     6841 agtaataaca gacgaaaaac tttctaaaca aaatatagct acattagaaa atgctgggat
     6901 aatggttaag gtagtttcgt aagaggttaa gtgtatgaat caagatagga ataaactgct
     6961 ctctaaaatt gcttatctgt attatattga aaacttaaat cagtcacaaa tagcagcaaa
     7021 attaggaatt tatagaacct ctattagtag aatgttaaca gaagcaagga atgcaggaat
     7081 tgttaaaatt gaaatagaga attttgatac aaatatgttt aagttggaaa attatgtaaa
     7141 agaaaaatat ggtctggaaa gtttagaaat tattccaaat gaatttgatg ataatccaac
     7201 aattttatct gaaagaattt ctcaagttgc agcaggtgtc cttaggaatt taattgatga
     7261 taatatgaaa attggctttt cttgggggaa aagtttaagt aatttagtag atttaattca
     7321 cagtaaaagt gtccgaaatg ttcacttcta tcctctagca ggtggtccta gtcacataca
     7381 cgctaaatac catgtgaata cactgattta tgaaatgtct agaaaatttc atggagagtg
     7441 tacatttatg aatgcaacga ttgtgcaaga aaataaattg ttagcagatg gtattttgca
     7501 atcaagatat tttgaaaatt tgaaaaatag ttggaaagat ttagatatag ctgtagtcgg
     7561 aattggtgat tttagcaata aaggaaaaca tcaatggtta gacatgctta cagaggatga
     7621 ctttaaagaa ttgaccaaag ttaaaactgt aggagaaatt tgttgtcgat tttttgattc
     7681 aaaaggtaaa gaagtctatg aaaacttaca ggaaagaacg atagcaatct ctttggaaga
     7741 tttaaaaaat attcctcaaa gtttagctgt tgcttacggt gatacgaaag tatcttcgat
     7801 tctttctgtc ttgcgtgcta atttagtaaa tcatttgatt acagacaaaa atacaatttt
     7861 aaaagttttg gaagaagatg gggatttgac ttttagagag attctaggtg agtgaaaatg
     7921 atagactgat tcagtttatc gtttttcttt ttagttgatt gcacatttgt gcttatataa
     7981 acaaaaatag tttatctgtt gtttttggat tgacaacttt at
//
LOCUS       DAVLXF010000084         8020 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLXF010000084 DAVLXF010000000
VERSION     DAVLXF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.084"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            5..1405
                     /gene="galT"
                     /locus_tag="VYO12_001755"
     CDS             5..1405
                     /gene="galT"
                     /locus_tag="VYO12_001755"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1588308.1"
                     /translation="MKNRVLALIGEEGTDRISDENDLKQIKDYLVEIALKNGKIKDLI
                     EEKECLGAELMNFIVPLPSRLNDIFWSSYDISPQEAVEEFYKLSKDSDYIKTSAIAKN
                     IEFRASTKYGELEITINLSKPEKDPKTIAAEKLVKATNYPKCLLCMENEGYQGRINYP
                     ARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIFLNSQHVPMAITSKTFEQLLEIVDIL
                     PGYFAGSNSDLPISGGSILSHNHYQGGKHIFPMEKAKFESEFRFKDFEDVNAGIVKWP
                     MSVIRLQSENKNRLLDLATKILNKWREYSDLEVDIIAMTEDVPHHTVTPIARKVDGRY
                     ELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKENIGLIEVMGLAILPPRLKPELEEVGK
                     YLLGEDNAIADYHLEWADQLKEKYPRINKEEVNSVVQHEAGQVFARVLEDAGVYKNTP
                     SGHEAFMRFVKSVGIN"
     gene            1418..2428
                     /gene="galE"
                     /locus_tag="VYO12_001756"
     CDS             1418..2428
                     /gene="galE"
                     /locus_tag="VYO12_001756"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1588309.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            2608..3267
                     /locus_tag="VYO12_001757"
     CDS             2608..3267
                     /locus_tag="VYO12_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1588310.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3292..4359
                     /locus_tag="VYO12_001758"
     CDS             3292..4359
                     /locus_tag="VYO12_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1588311.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4463..5473
                     /locus_tag="VYO12_001759"
     CDS             4463..5473
                     /locus_tag="VYO12_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588312.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5485..7176
                     /locus_tag="VYO12_001760"
     CDS             5485..7176
                     /locus_tag="VYO12_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1588313.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7179..7889
                     /locus_tag="VYO12_001761"
     CDS             7179..7889
                     /locus_tag="VYO12_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1588314.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2695 a         1132 c         1578 g         2615 t
ORIGIN      
        1 atatgtgaaa aatagagtac tagcgttaat aggtgaggag ggaactgatc gcatttccga
       61 tgaaaatgac ctgaaacaaa taaaagatta cttagtggaa atcgccttaa aaaacggtaa
      121 aattaaagat ttaatcgagg aaaaggaatg tttaggagca gagttaatga atttcatagt
      181 tcctttacct agtcgattaa atgatatttt ttggagttca tatgatatat cacctcaaga
      241 agcagttgaa gagttttata agttaagtaa agatagtgat tatattaaaa cttctgctat
      301 tgctaagaat attgagtttc gagcatcaac taaatacgga gagctagaga ttacgataaa
      361 tttgtcaaaa cccgaaaaag atccaaaaac aattgctgct gaaaaattag taaaagccac
      421 taattatccc aaatgtttac tatgtatgga aaatgaagga tatcaggggc gaataaatta
      481 tccagcacgt tcgaatcata gaattattcg attgaaactt ggtgacgaag tatggggatt
      541 ccaatattct ccctacttat actttaatga gcatgcgata tttttaaata gtcaacatgt
      601 accaatggct attacctcta aaacatttga acaattattg gagattgttg atattttgcc
      661 aggatatttt gctggttcaa attctgatct tcctatctca ggaggctcca ttcttagtca
      721 taaccactac cagggaggga aacatatttt cccaatggaa aaagcaaaat ttgagagtga
      781 atttcgtttt aaagactttg aggatgttaa tgctggtatt gtaaaatggc caatgtcagt
      841 aattaggtta caaagtgaaa ataaaaatcg cttgttagat ttagctacta aaattctaaa
      901 taagtggaga gaatattcag atttagaagt agacatcatt gctatgactg aagatgtccc
      961 acaccatacg gtgactccta ttgctcgtaa agtggacggg agatatgaat tagatattgt
     1021 tctaagagat aatcatacta ctgaacagta cccagatggt gttttccatc ctcatcaaga
     1081 tgtacaacat attaaaaagg aaaatatcgg gttaatcgaa gtgatgggac ttgcgatatt
     1141 accacctcgt ttgaaaccag agttagaaga agttggaaaa tatcttttag gagaggataa
     1201 cgcaattgcg gattatcatt tagaatgggc cgaccaattg aaagaaaaat atcctcgcat
     1261 taataaagaa gaggttaata gcgtagttca acacgaagca ggacaagtgt ttgcgagagt
     1321 tctggaagat gcaggagttt ataagaatac accgtctggg catgaagcgt ttatgagatt
     1381 tgttaagtcg gttggaatta attaggagga aacaaaaatg gcaatattgg taacaggcgg
     1441 agctggttat attggtagcc ataccgtagt agaattacta aatttaggaa aggaagtcat
     1501 cattgtcgac aacctttcga actctagcat cttggtgcta gaccgtattg aagcaattac
     1561 aggaatacgt cccgtgtttt acgaattaga tgtttgtgat aaaccagcat tgagaaaggt
     1621 atttgaacaa gaatcgattg atgctgcaat tcattttgca ggttataaag ctgtcggaga
     1681 atccgtgcaa aagcctgtga tgtactacaa aaataatatt atgagtacat tggcacttgt
     1741 tgaagtgatg tcagaattta atgttaaaaa gattgtcttt tcttcaagtg cgactgtata
     1801 tggaattaac aatcagtcac ctctaattga gactatgcaa acaagtgcga caaatcctta
     1861 tgggtatacg aaagtgatgc ttgagcaaat tttaaaagat gttcatgtgg cagattcaga
     1921 atggagtatt gcgttgcttc gttatttcaa tccaattggt gctcatgagt ctggtttgat
     1981 tggggaagat ccctcaggaa ttcctaacaa cttgatgccc tatattgcac aagtagcggt
     2041 aggtaagctg tcagaactaa gtgtgttcgg aaatgattat gatacgcttg acggtactgg
     2101 tgtgcgcgat tatatccatg tagtagattt agcaatagga catataaaag ctttagaaaa
     2161 agtatctgaa aaaacagatg tttatatcta taacctaggc tcgggagaag gcactagtgt
     2221 attacaactt gtaaatacat ttgaaagtgt taataagatc cctattcctt ataaaatagt
     2281 tccaagacgt tcaggagacg ttgcaacttg ttatgcaaat gcagacaaag catataagga
     2341 attaaattgg aggacaacaa aatcgattga agacatgtgt agagatacat ggaattggca
     2401 atcaaaaaat cccaatggct ataattgatg atactgtcta tatgagtgct gtattttgga
     2461 aaaattactt aaccttttaa tgaaatgcat atttaatgaa agataaatta aaaaaaatgt
     2521 ttgacagcgc ttgacaaagg aaacggtttc attgtataat gtaaacacaa tgacaccgat
     2581 gtcaaatata aaaaagaagg tctttatatg aaaaacttaa caaaaattaa gttcaaagaa
     2641 aatggggaat ttaatcattt ccctgggaat acagttgtag caaatcttta tactaaacaa
     2701 gatttgatgg aagttgttga tattattcaa tcacgttata gagaattacc atttatcgat
     2761 aagtttactt taactccaag gaattctatt cacatgactg taattgaatt gttgtgccat
     2821 gaaaatcgtg aaacggagtt ttggagcagt aatcttcctc tagatacacc tttacaggaa
     2881 atacatgatt actttgctaa acaacttgaa atttttccat tgttggatga agaaattcat
     2941 atgcgtataa ctgaaatggg aaaacaaaac atactagttg aacctgcaga tgaagcttct
     3001 gcaaagagat tagaagaaat tcgtacttat gtctcagaaa aagcaggtgt ttgtttccct
     3061 aatcatgata gatatcaatt ccatatttca attgggtatc ttcggattcc tctaaccgaa
     3121 gaggaggaag aagagtttac taaagtcaga gcagaattaa ctgaaatttt attagagaag
     3181 attccaacta ttactgtaaa ccgtattgac tatactgtat ttgaagatat gaggcaattt
     3241 gttccatatc acgaaaaatt taaataaaat aataaaaaag gagaacttat catgaaaaaa
     3301 atgaaagttt ggtctactgt acttgcaacg ggagttgctc ttactacact tgctgcttgc
     3361 tctggaggtt caaattctac gactgcttct tcatctgaag aaaaagctga taaaagtcaa
     3421 gaattagtta tctattcgaa ctcagtctca aatggtcgtg gtgattggtt aactgctaaa
     3481 gcaaaagaag ctggttttaa tataaaaatg gttgatatcg ctggcgctca attagcagac
     3541 cgtgttattg ctgagaagaa taatgcagtt gcagatatgg tatttggaat tggtgctgtt
     3601 gactcaaata aaattagaga tcaaaaatta ctagtacagt acaagcctaa atggttagat
     3661 aaaattgatc aatctttatc agataaagat aattattata atcctgtgat tgttcaacca
     3721 ttagttttaa ttggggcgcc tgatgtaaaa gaaatgccta aagattggac tgaattaggt
     3781 agtaagtata aaggtaaata ttcaatttat ggtcttcaag gtggtacagg acgggcaatt
     3841 ctagcaagta tcttagttcg ttaccttgat gaaaaaggag atttaggcgt atcagaaaaa
     3901 ggatgggaag ttgcaaaaga atactttgca aatgcctata ctcttcaaaa aggtgaaagc
     3961 tcagttgtta aagcgttaga taaagaaagc ccaattcaat acggtatgat ctggggttct
     4021 ggagcgttaa ttggacaaaa agaacaaaat gttgaattca aagttatgac tcctgagatt
     4081 ggtgtaccat ttgtaactga acaaactatg gttttaagca ctagtaaaaa acaagcgtta
     4141 gctaaagaat ttattgattg gtttggtcaa tcagaaattc aagtagaata tagtaaaaaa
     4201 tttggatcta ttcctgccaa taaagatgcc ctaaaagagt tacctgaaga tacgaaaaaa
     4261 tttgttgatc aagtgaaacc acaaaatatt gactgggaag ctgttggaaa gcatttggat
     4321 gaatgggtag aaaaagctga attagaatac gtaaaataag aaataagagt tattttttat
     4381 gtccaagtct ctctattatt tctaaattta aggaagaaat aaagagaggc ttttggtttt
     4441 agtaatagag ggagttagtg agatgattaa atttgataat attcaaatta aatatggtga
     4501 ttttgttgca attgataatc tgaatttaga tatacatgaa ggggaattct ttacatttct
     4561 tgggccttca ggatgtggta aatcaactac tttgagagca ttggtaggtt ttttagatcc
     4621 atcatcagga agtattgaag ttaatggaac agatgtcact catttggaac ctgaaaagcg
     4681 tggaattggt attgtatttc aatcttatgc gctatttcca actatgactg tttttgataa
     4741 tattgcattt ggtttaaaag ttaagaaggt agctccagat gttattaaag ctaaagtatc
     4801 agcagtggca gcaaaaatta agatctctga tcaacagtta cagcgtaatg tatcagaatt
     4861 atctgggggt caacaacaaa gggtagcatt ggctcgtgct ctggttcttg aacctaaaat
     4921 tctttgtcta gatgaaccat tgtcaaacct tgacgcaaaa ttacgtgtag atttgagaaa
     4981 agagttgaaa agacttcaaa aagagttagg tattactact ttatatgtta ctcatgatca
     5041 agaggaagcc ttgactttat ctgatagaat tgcagtcttt aacaatggat acatcgaaca
     5101 ggtcggtaca ccagtagaga tttatcataa ttctcaaact gaatttgtat gtgattttat
     5161 tggagatatt aatgttttga ccgatgaaac agtccacgaa gtattactga aaaatacaag
     5221 cgttttctta gaggataaaa aaggttacat tcgattagag aaagttcgat tcaatcgtga
     5281 aactgaacaa gattttattc taaaagggac aattattgat gttgagtttt ctggagttac
     5341 aattcactat acaataaaag tttctgaaag tcagattctt aatgtaacaa gtattgatag
     5401 tcaggctgct attagatctg tcggagaaag tgtggaatta tttatcacac catcagacgt
     5461 tctgcaattt taaggaggtg cagtatgcgt cataaattaa atttaaaaga ttggcttatt
     5521 cgtttagggt taatctggtt cttagtaaca tttattattt atccaaactt tgatctagta
     5581 gtgaatgtat ttgtaaaagg aggagaattt tcccttgatg ctgtacatcg tgttctaaaa
     5641 tctcagaggg cacttcagag tattatgaac agttttaagt tagcattttc actcattatt
     5701 acagttaatg tcgtaggtat tctttgtgtt ctatttacag agtactttga tattaaaggt
     5761 gctaaaattt taaaattagg ttatatgacc tctttaattt atggaggagt ggttttagcg
     5821 actggatata aatttgtcta tggtccttat ggattgatta caaaattttt acaaaatgtt
     5881 atcccttctt tagactctaa ctggtttatt gggtatggtg cagtcttatt cattatgaca
     5941 ttttcaggaa ctgctaatca tacattgttt ttaacaaata caattcgaag cgttgactat
     6001 cacactattg aggctgctcg aaatatggga gcaaaaccat ttactgtttt ccgaaaagta
     6061 gtgttaccaa ccttaattcc aactctattt gcacttacta ttatggtttt tcttagtggt
     6121 ttatctgcag tagcagcacc catgattgtt ggtggtaaag aatttcaaac tataaatcca
     6181 atgattatta catttgcagg gatggggaat tctcgtgatt tagctgcctt acttgcaatt
     6241 attttaggta ttgcaactac aattttgctt actatcatga ataagataga aaaaggtgga
     6301 aattatattt ctatctctaa gactaaagcg cctcttaaaa aacaaaaaat tgcgtctaag
     6361 ccttggaata tcattgctca cattgtagca tatggattgt tcacagtttt catgcttcca
     6421 ctaattttta tagtattata ctcatttaca gatccagttg caattcaaac aggtaactta
     6481 acattatcaa actttacttt agaaaattat cgcttattct ttagtaatag tgcggcattc
     6541 tctccattct tggtcagctt tatttattct attattgctg cgacaacagc aacaattctc
     6601 gcagttgtat ttgctcgtgt tgtcagaaaa cataaatctc gttttgattt cttatttgaa
     6661 tatggtgctc tacttccttg gttactacca agtacacttt tagcagtaag tttattattt
     6721 acttttaatc agccacaatt tcttgtcttg aatcagattt tggtaggtag tttggtaatt
     6781 ctacttattg catatatagt tgtaaaaatc ccattttctt atagaatggt acgtgctatt
     6841 ttatttagtg ttgatgatga gatggaagat gcagcaagaa gtatgggtgc ttcacctttt
     6901 tatactatga tgaaggttat cattccattt attttaccgg ttgttctctc tgttattgct
     6961 ttaaacttta actctttatt aactgacttc gacttatctg tattccttta ccacccccta
     7021 gctcaaccat taggtattac gattcgatct gcaggtgatg aaacagcaac atctaatgca
     7081 caagctctgg tatttgttta tacaattgtt ctgatgatta tttctggaac ggtattatac
     7141 ttcacacaaa gaccggggcg taaagtaagg aaataatcat gacagccact agtcttgggt
     7201 tatcaaatat tgaaatagtt gtcaggattg ttttatcagt agtcattggt agtataattg
     7261 gtttagagag agggagcaaa tcccagcctg caggcatccg aacttatagt attgtttgtc
     7321 tagctgcatg tttgattatg atgacgaatg aatacgtatc ttataaattt gggacaggag
     7381 atcctacacg attaggagct caagttatat caggtgtggg ttttctaggc gctggaacga
     7441 ttcttattac agataaaaag aaaattacag gtctgacaac tgcagcaggc atttgggctt
     7501 cggcaggaat tggattagct attggagtag gtttttatga gggagctctt ttagtagcca
     7561 tttctgtttg gggtgtgata tccatgttcc aaccactaaa aaaatatcta caaaatcgtt
     7621 ctaaaatgat tgaattgtat atagtagtta aatctacaga agcctataat cgtgtattgg
     7681 tttattgtgc agaaaatggt atcagaatga ccgattcaag aacagcgttt ggagatgtta
     7741 attctgacag aattgaatac tttgatgttc cagataaaaa aatagcgtca tttattactc
     7801 tggaattatc aggtagattt gaacatcttc gactgatgga agaaattgct aatattgtag
     7861 gtgttattta tgtcgaagaa attagctgaa atttagtaaa caaagggaca gatgtttttg
     7921 tccttttgtt ttttagatat tttaatcatc taatttttga gtagattttt gctcagaggt
     7981 ttttatgaaa gattataaaa tcaattttga tttaggtaaa
//
LOCUS       DAVLXF010000085         7895 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLXF010000085 DAVLXF010000000
VERSION     DAVLXF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.085"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            212..967
                     /locus_tag="VYO12_001762"
     CDS             212..967
                     /locus_tag="VYO12_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1588315.1"
                     /translation="MIQLTASTWNINLRSRKGKQIPEMIAQELAQQNSDIICLTEYVK
                     TDNHSQFCSSLQTNGYTIFEDVRTIDFGNEILIAIKSDLVNDCQVITIDNDDNNPNFL
                     HVRVTIEGQELNIIGVRIKIGGENVFEDFKHRKTQLDKLIFNLPIHENTIILGDFNNG
                     FFKQNDDTHSYQGKPREFYSYPLLKSEMNKAGLTVHTPSEENSWKYCKLDHIIGNIPI
                     SNVCYSWRFLENPDYQNKVGFPDHAILSATISL"
     gene            1007..1225
                     /locus_tag="VYO12_001763"
     CDS             1007..1225
                     /locus_tag="VYO12_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588316.1"
                     /translation="MSDNLKAYLFLLGFVILAGIAIFIFPQGHFFSNSILILGCIPLL
                     ISTYYFAKIKLLGFYELLKELLDKIKGI"
     gene            1231..2553
                     /locus_tag="VYO12_001764"
     CDS             1231..2553
                     /locus_tag="VYO12_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEV1588317.1"
                     /translation="MFKLRQSADLTRNNIISCYKLKIMHWLILSISACILFSTIVNIR
                     APTFVQILFIFLGGFIIYKIHLKIKIFRYDLEHYLIIRESLLYVLHTNRLYTTSKDGS
                     GYEKIVRSAVLEYEIDRQKGHVLIKALITGDEFSKKVQSLDDVLSDVLELELEEKIIR
                     PSFIEYHFYYIKPKRLVLQSHSQRQEIDSLDIDLGYGVNYNPVKCSHILVSGGTGSGK
                     SVLISVLILEFLKRQSTVYIADPKNSDLGSLSHYFGNKYVATTPNNIARIVRVVVEEM
                     QERYQVMRDNFQYGSNFTNHGFKPVWLIFDEMGAFQATGSDKKSREIITEVMDGIKQI
                     ILLGRQSGIFILVSAQQVNASTTLSTELRDNLGLRIALGANSSEGYHMVFGSATPDKL
                     KPIEEKGSGYLYIQGSGKESAQYWESPYLDTTQFDFIKELQLYVTKSK"
     gene            2643..2957
                     /locus_tag="VYO12_001765"
     CDS             2643..2957
                     /locus_tag="VYO12_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588318.1"
                     /translation="MKLNDFLKPELLGNKFIAVKGYTEVLDRETNKPVALRLNVSIQD
                     EDSDFFMEMIQVKVNTLSPTASVQEMANNKTCPVTLKDLNVGQYNGNLWFSCSNVILI
                     TK"
     gene            2995..3384
                     /locus_tag="VYO12_001766"
     CDS             2995..3384
                     /locus_tag="VYO12_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588319.1"
                     /translation="MQIFTYNDFLKDKEIVTFEQAEIILDELIKSSNIYDPEFQAYWK
                     ELIEYSAKYAEMRGKWRILTKEEQDTLDDTRTNIHNRIRDNLISIRGLAQINNKDTSW
                     FDKFHNDRKRMGDFANYINYIYAVNSR"
     gene            3403..4173
                     /locus_tag="VYO12_001767"
     CDS             3403..4173
                     /locus_tag="VYO12_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1588320.1"
                     /translation="MAIYYSVSDIHGHYNEFIKSLNKIDLSDKQSKLILLGDYIDYGS
                     QSFQVISKIIELEKIYPKQIITLFGNHEEWFYDWLILDNPTASAFPETIKSFFSTEEL
                     NYILKTNPNNFETGVRNEIRNNDKYIPFINWFQKRYEDSRYFETENQIFVHAGIDEEA
                     NNLWKDLTSPEIFTNKFPITTGRFYKDIISGHFASWEVAKDKSYLGKIYHDSKSHYFI
                     DGDVKNSKTIPVICYDTVKKSYQYSKNTSLLPKKFDRA"
     gene            4217..4990
                     /locus_tag="VYO12_001768"
     CDS             4217..4990
                     /locus_tag="VYO12_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1588321.1"
                     /translation="MKTLLIGDLHLKSQLILPIVDKIIQTHNIKRIIFLGDYVDLHGQ
                     TNNIQLYAKDLTFLYSWKIDKEQNGIDVINLIGNHDAYYLLGEQAPFSIQNLEVFFAV
                     KELLQDLKLQIAYQLDDYLVSHAGFNLIFDPKEWHFNLYTKEYEEELDILAYTIGPMR
                     GGKALGGSPLWAHFRELELLPNQDFPKQIVGHTPKESIDISKNVIGIDTFSLYIDKDN
                     KYQFIGNGDLLVYDQGHLEIISTNWATDETLKQLSKPQI"
     gene            5174..5956
                     /locus_tag="VYO12_001769"
     CDS             5174..5956
                     /locus_tag="VYO12_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEV1588322.1"
                     /translation="MTSYNKKIVRTSSYIEIWEYEKPIFSKGKSILDTNTENKEKAKR
                     RTFDELTPLEQEQRLERMKKTRLEAKWNLLRLVDCNFDDTTSFLTLTTKENISDRNHF
                     KDSLKTFIKRFNYHIFNTKKSKLKYIAVLERQKRKAWHSHILLFSVPYIPHKQLLELW
                     GHGAVWINKVDVDSKENRGRYVTKYFEKGIGQELLENFGKQAYFSSRNLKKPDEDKFY
                     TYEDFNYDSSVVLYETEYTSKVYKYGQYFDNHVKYKKIRLDE"
     gene            5970..6173
                     /locus_tag="VYO12_001770"
     CDS             5970..6173
                     /locus_tag="VYO12_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEV1588323.1"
                     /translation="MIATVTKNDLIALGFFEGTSKRIIRQGKELLITRGFTVYQNKRI
                     GTIPATIATELLVFDVQNSTLKE"
     gene            6177..7310
                     /locus_tag="VYO12_001771"
     CDS             6177..7310
                     /locus_tag="VYO12_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1588324.1"
                     /translation="MAIRKGKDGNWVVDISNGFDPITLKQRRLVRKGYKTKKEAIEAE
                     HYLRSVELKERFYGAKITIPILYELLKEEDSINHRKASYINTQDNNYNKHIKDYFSMV
                     DNVGKLTYEDIYQFREHLRQKVAKNSDKPLSTNTINKIMILLKKIFDVGLRKGYYSSN
                     PAKLIKKLPIEKTKMQFWTVKEFQKFLTLFEPEEYNIKLLFTVLFFTGLRLGEALALT
                     WQDIDFSSNTIHITKSVYVNKGVTHISTTKTKAETRRIVINKKLSQELQDWKKRQKHL
                     LEQFTNNSLTLQVFQNSPITITKNAIEKQYKKIIERDSTLKKIRIHDFRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKDLADKLDDLL"
     gene            7351..7788
                     /locus_tag="VYO12_001772"
     CDS             7351..7788
                     /locus_tag="VYO12_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1588325.1"
                     /translation="MDTSQVIKAHEVAKKAHFGQIDRAGIDYIKHPETVASFVATDEE
                     KAVAYLHDVIEDTSLTLLDLKKEGFSKNIIEAVDILTKKKGQDYQSYLNLVKKNELAR
                     VVKLADLRHNSDLTRLPLITEKDLERNKKYSSAIRFLRAKKKS"
BASE COUNT         2954 a         1245 c         1298 g         2398 t
ORIGIN      
        1 cttcttttaa ttttgtacaa ttaaaatgta caaaaaaata ctgagaggag gaatagcaat
       61 gaactttttt gaccaagaag ccttagatta cttctatgag gaattggaaa gctactatga
      121 tgaagacgaa ctaggagaaa tctttgatgg attcgaagga tagcaggagg aggtgcctcc
      181 tcctgcggac taacttttag gaggaaattt tatgatacaa cttacagcat ccacttggaa
      241 tataaatcta cgttcaagaa aaggaaaaca aattcctgaa atgattgcgc aggaactagc
      301 tcagcaaaat tcagatatta tctgtctgac agagtatgta aaaacagata atcattctca
      361 attctgttct agccttcaaa ctaatggtta tacaatcttt gaagatgtgc gtactataga
      421 ttttggaaat gaaattctta ttgcaataaa gagtgattta gttaatgatt gccaagtcat
      481 tactatcgat aatgatgata ataatccaaa ctttttacac gttagagtga caattgaagg
      541 acaagaatta aacattattg gagttcgtat caagatagga ggggaaaatg tttttgaaga
      601 tttcaaacac agaaaaacac aactagacaa gctaatcttc aatttaccaa ttcatgaaaa
      661 cacaattatt ctaggtgact ttaataatgg ctttttcaag cagaatgatg atactcattc
      721 ctatcaaggt aaacctagag aattttactc ctatccatta ctaaaatcag aaatgaataa
      781 agcaggttta actgtacata ctccgtctga ggagaactct tggaaatatt gtaaattaga
      841 ccatattata ggaaacattc ctatttctaa tgtgtgttat tcttggagat tcttagaaaa
      901 tcctgattat caaaataaag tcggtttccc cgaccacgct attttatcgg cgactatatc
      961 tttatagttt tccttaaaat aattgcttat tagaggaggt aatgaaatga gtgataattt
     1021 aaaagcatat ctatttcttt taggatttgt gatattagct ggcatagcaa tttttatatt
     1081 ccctcaagga catttctttt cgaattctat ccttatcctt gggtgtattc cactacttat
     1141 ctcaacatat tatttcgcaa aaatcaagct cttagggttt tacgaacttt taaaagagct
     1201 tttagataaa ataaaaggta tctaactaat atgtttaaat taagacaaag tgctgattta
     1261 acaagaaaca atataatttc ctgttataag ttaaaaataa tgcactggct gatactaagt
     1321 atctctgcct gcattctatt ttccactatt gttaatatta gggctccaac ttttgttcaa
     1381 attctcttca tatttttagg tggatttatt atctataaaa ttcatctgaa aataaaaatt
     1441 tttcgatatg acttagagca ttaccttatt atacgtgaga gcttacttta tgtactccac
     1501 acaaacagac tttataccac atctaaagat ggttcaggct atgaaaaaat cgttagaagt
     1561 gcagtattag aatacgagat agatagacaa aaaggtcatg ttcttatcaa agctcttata
     1621 acgggcgatg aatttagtaa aaaagttcaa tctcttgatg atgtactttc tgacgtgctt
     1681 gaactagagc ttgaggaaaa gattattcga cctagtttta tagaatatca tttctattac
     1741 ataaaaccaa aaaggttagt tctacaatct cacagccaaa gacaagagat agatagcctt
     1801 gatatagatt taggatatgg tgtaaattat aatcctgtca aatgctcaca tattttagta
     1861 tcgggtggaa ctggaagtgg gaaatccgtg cttatatctg ttttgatttt agaatttcta
     1921 aaacgtcaat caacagttta tatagctgac cctaagaact ctgacctagg tagcttgtct
     1981 cattactttg gaaataaata cgtagcaacc accccaaata atatagctcg cattgtgaga
     2041 gtcgtagttg aggaaatgca ggaacgatat caagtcatgc gtgataactt tcagtatggt
     2101 tcaaatttta ctaaccatgg atttaagcca gtatggctta tatttgatga aatgggagca
     2161 tttcaagcta caggttcaga taaaaaatca agagagatta tcactgaagt tatggatggg
     2221 attaaacaaa tcattctact tggaagacag tcaggcattt ttatcctagt gtctgcccaa
     2281 caagtaaatg ctagtactac tttaagtact gaactcagag ataatttagg attgagaatt
     2341 gctcttggtg ctaattctag tgaaggctat cacatggtct ttggttcggc tactcctgat
     2401 aagttaaaac ctattgaaga aaaaggttca ggctatcttt atatacaagg ctctggtaaa
     2461 gaatctgccc aatactggga aagcccttat cttgatacaa ctcaattcga ttttataaaa
     2521 gaactacaat tatacgtaac aaagtcaaaa taatttcaac agatattttt gcttaaattt
     2581 aatagatttt cagcaaaaat atctgttcct catcgctctg cgatgataga aaagaggtac
     2641 acatgaagct aaatgatttt ttaaagccag aactgcttgg caacaaattt atagcagtaa
     2701 aaggctatac agaagtatta gacagagaga ctaataagcc cgtagcttta cgacttaacg
     2761 tgtcaattca agatgaggat tctgatttct ttatggagat gatacaagtc aaagtaaata
     2821 cattatcccc tactgcttct gttcaagaaa tggcaaataa taaaacctgt cctgttactc
     2881 taaaagattt aaacgtaggt caatacaatg gaaatttatg gttttcttgt tctaatgtaa
     2941 ttctaattac taaataatgt gttataatta tttcaaaaat taaaggagct tactatgcaa
     3001 atttttactt ataacgattt tctaaaagac aaagaaatcg taacttttga acaagctgaa
     3061 ataattttag atgaacttat aaaatcatca aacatttatg accctgaatt tcaagcatac
     3121 tggaaagaac ttatcgagta cagtgcgaag tatgctgaaa tgagaggaaa atggcgaata
     3181 cttacaaaag aagagcaaga tacacttgat gatacaagaa ctaatattca taatagaatt
     3241 agagataacc ttatatctat tagaggcttg gctcaaatca ataacaaaga tacctcttgg
     3301 ttcgataagt ttcataacga tagaaaacgt atgggtgatt ttgctaatta tataaattac
     3361 atatacgctg taaattcacg ttgattatag aaagaatctt atatggcaat ttattattcc
     3421 gtatctgata tacatgggca ctacaatgag tttattaaat ctctcaataa aatagattta
     3481 tctgataaac aaagtaaact tatcttactt ggagactata ttgattacgg ttctcaatct
     3541 tttcaagtca tttctaaaat aattgaactt gaaaagattt atccaaaaca aattattaca
     3601 ctttttggaa atcatgaaga atggttttat gattggttga ttttagataa tcctactgct
     3661 tctgcgtttc cagaaacaat aaagagtttc ttctctacag aggaattgaa ctatatttta
     3721 aaaacaaatc ctaacaactt tgaaacaggt gttagaaacg aaattagaaa taatgataag
     3781 tatattccat ttataaactg gttccaaaaa cgatatgaag atagtcgtta ttttgaaaca
     3841 gaaaatcaaa tttttgtcca tgctggcatt gatgaagaag ctaataatct ttggaaagac
     3901 ctaacgagtc cagagatatt taccaacaaa tttcctatta caactggtag attttataaa
     3961 gatattatct ctgggcattt tgcttcttgg gaagtagcta aagataaaag ttacctaggt
     4021 aagatttacc atgatagtaa aagtcattac tttattgatg gagatgtaaa aaatagcaaa
     4081 acaatacctg ttatctgcta tgatacagtt aaaaaatcct atcaatattc aaaaaacact
     4141 tctctattgc ctaaaaaatt tgatagagct taattctatt cttagactaa tatcaaaaat
     4201 aaagagggga atatatatga agacattatt aattggtgac ttacatttaa aatctcaact
     4261 aattttaccc attgttgaca aaattatcca aacacataac attaagagaa tcatttttct
     4321 tggtgattat gttgatttac atggtcaaac taacaatatc caactttatg ccaaagacct
     4381 cactttttta tactcttgga aaatagataa agagcaaaac ggaatagatg taattaatct
     4441 tataggtaat catgatgctt attatttact gggagaacaa gctcctttct caattcaaaa
     4501 tttagaagtg tttttcgcag taaaagaatt gttgcaggat ttgaaattac aaatagctta
     4561 tcaactagat gattatttag tcagtcacgc tggtttcaat ctgatatttg acccaaaaga
     4621 atggcatttt aacctttata ccaaagagta tgaagaagaa ttagatatcc tagcttatac
     4681 aataggacca atgcgtggtg ggaaagctct tgggggttct cctttatggg cacattttag
     4741 agaattagaa ttgcttccca accaagactt tcctaaacaa attgttggac atacccctaa
     4801 agaatctatt gatatttcta aaaatgttat tggtatagat actttttcat tatatattga
     4861 caaagataat aaatatcaat ttattggtaa cggagatttg ctagtctatg accaaggcca
     4921 tcttgaaatc atttcaacca actgggctac agacgaaaca ttaaaacaat tatctaaacc
     4981 acaaatttaa ctactacttc tcatcatgtg actaagcgaa actgctgact ggaggtcagc
     5041 aagctggaag cgtgtttagc ggagcgatgc ttagaaacac atgatagaag tgtggaggag
     5101 ctttgctcct cctagtttat ttattatata gacaatcttt tggcgaaagc caacctaaaa
     5161 ggaactattt tttatgacct catataataa aaaaatagtc cgcacttctt cctatattga
     5221 aatttgggaa tatgaaaagc caatattttc aaaaggaaaa tctattttag atactaatac
     5281 tgaaaacaag gaaaaagcaa aacgcagaac atttgatgaa ctaacacccc tagaacaaga
     5341 acaacgtctt gaacgtatga agaagactcg cttagaagcc aagtggaact tactccgtct
     5401 agttgattgt aattttgatg atacaacttc ttttcttaca ctgaccacta aggaaaatat
     5461 cagcgataga aaccacttca aagactcact caaaacattt attaaacgct ttaactacca
     5521 catttttaat accaagaaaa gtaaattaaa atatatagca gtattagaga ggcaaaaacg
     5581 taaagcatgg catagtcata ttttgctatt cagtgttccc tatattccac ataagcaatt
     5641 attagaactt tgggggcatg gtgcagtatg gataaataaa gttgacgtcg acagcaaaga
     5701 aaatcgcggt cgatatgtca caaagtattt tgaaaaaggt attggacaag aattgttgga
     5761 aaacttcgga aagcaagcct atttttcatc acgcaacttg aaaaagcctg atgaagataa
     5821 attttacact tatgaagatt ttaactatga tagttcagtc gtgctatatg aaacggaata
     5881 cactagtaag gtctataaat acggtcagta tttcgataat catgtaaagt acaagaaaat
     5941 aagactagat gaataaagga gtaagtcaca tgatagcaac tgttactaag aatgacctaa
     6001 ttgccctcgg tttctttgag ggaacatcta aacgtattat ccgccaaggg aaagagctac
     6061 tgataacccg aggctttaca gtctatcaga acaagcgaat tggtactata cctgctacta
     6121 ttgctaccga attactcgtt tttgatgttc aaaacagtac cctcaaggag taaaatatgg
     6181 caattagaaa aggtaaagac ggtaattggg tagttgatat tagcaacggt tttgacccaa
     6241 taacactaaa gcaacgtaga cttgtcagaa agggctataa gacgaaaaaa gaagctattg
     6301 aagcagaaca ctacttacga agtgtagagt taaaagaacg cttttatgga gctaaaataa
     6361 ctatccccat actatatgaa ctacttaagg aggaagatag tataaatcat cgaaaagcaa
     6421 gctatatcaa tacacaagat aataattaca acaaacacat caaagattat ttttctatgg
     6481 tcgataatgt cggaaaacta acctatgaag acatctacca atttagagaa catcttaggc
     6541 aaaaagtagc taaaaattct gataaaccgt taagcacaaa tactattaac aaaatcatga
     6601 ttttactcaa aaaaattttt gatgttggtt tgcgaaaagg ctattactcc tccaatccag
     6661 caaaattaat aaaaaagttg ccaattgaaa aaacaaaaat gcaattttgg acagtcaaag
     6721 aatttcagaa gtttttgaca ctatttgaac ccgaagaata caatatcaaa ctactcttta
     6781 ctgttctatt ctttacagga ttaagacttg gggaagctct tgccctaaca tggcaagaca
     6841 tcgacttttc aagtaataca attcatatca cgaagtctgt ctatgttaat aaaggagtaa
     6901 ctcatatcag cacaacaaaa actaaagcag aaacaagaag aattgttatc aataaaaaat
     6961 taagccaaga attacaagat tggaaaaagc gacaaaaaca tctattagag caatttacca
     7021 acaatagcct aacattacaa gtgtttcaaa atagtcctat cacaattaca aaaaatgcta
     7081 ttgaaaagca gtacaagaaa atcattgaac gtgatagcac actaaaaaag atacgcatac
     7141 acgattttag gcactcacac gcttccctgc tgattaacca aggggaagat tatctagttg
     7201 taaaagagcg actaggacat gcttctataa caaccacgat tgatacctac tctcacttgt
     7261 acccaagtaa gcaaaaggat ttagctgata aactagatga tttactataa agtaattgag
     7321 ttttataaat ggaaagttta taattaaatc atggatacat cacaagtaat aaaagcacat
     7381 gaagtagcta aaaaagcaca ctttggacaa attgatagag caggaattga ctacattaaa
     7441 caccctgaaa cagtagctag ttttgtcgca actgatgaag agaaagctgt cgcctatcta
     7501 catgatgtaa ttgaagatac ttcactaacc ctactagacc taaaaaagga aggtttttca
     7561 aaaaacatta tagaagccgt cgatatactc acaaagaaaa aaggacaaga ttatcaatct
     7621 tatctcaatc tagtaaagaa aaatgaactc gctagagttg taaagttggc ggatttacgg
     7681 cataattcag atttaacaag actaccatta attactgaaa aagacttaga gagaaataag
     7741 aaatacagtt ctgctattag atttttaaga gcaaagaaaa aaagttagta actaaactat
     7801 aaaattttat aaagagaaaa caaaagacaa ggcttataaa ctttgtcttt attactatac
     7861 tggcggtcgg tctcgaactg gcacatcctt gcaga
//
LOCUS       DAVLXF010000086         7711 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLXF010000086 DAVLXF010000000
VERSION     DAVLXF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.086"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..743)
                     /locus_tag="VYO12_001773"
     CDS             complement(<1..743)
                     /locus_tag="VYO12_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1588326.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGFLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMT"
     gene            complement(758..1606)
                     /locus_tag="VYO12_001774"
     CDS             complement(758..1606)
                     /locus_tag="VYO12_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1588327.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(1603..3078)
                     /locus_tag="VYO12_001775"
     CDS             complement(1603..3078)
                     /locus_tag="VYO12_001775"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1588328.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(3401..4756)
                     /locus_tag="VYO12_001776"
     CDS             complement(3401..4756)
                     /locus_tag="VYO12_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1588329.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMAYKTEL
                     S"
     gene            complement(4766..5083)
                     /locus_tag="VYO12_001777"
     CDS             complement(4766..5083)
                     /locus_tag="VYO12_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1588330.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(5100..5417)
                     /locus_tag="VYO12_001778"
     CDS             complement(5100..5417)
                     /locus_tag="VYO12_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1588331.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(5432..6328)
                     /locus_tag="VYO12_001779"
     CDS             complement(5432..6328)
                     /locus_tag="VYO12_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1588332.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(6331..7593)
                     /locus_tag="VYO12_001780"
     CDS             complement(6331..7593)
                     /locus_tag="VYO12_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1588333.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDILLYLPFVIAIEKRFKLLED"
BASE COUNT         2653 a         1697 c         1079 g         2282 t
ORIGIN      
        1 gtcattccca tgacaaagaa taacatccct ttaaaacagt atcttaccca attttcaggt
       61 aagacttttg atacaaccgt ccccaatacc cctccgcata tagaagcaag aaaaataact
      121 ggaacaggtg ctaaatgata ctgatttaaa cctgtaacca caacaattgt agccaaatga
      181 gacaactggg aaaagaaaat aattgcaaga gaatacatcg ttgcttcttt tatcgaaata
      241 gaaaaaaaca ccatcaataa agatacattg agcggaccac caccaatccc taaaaaacta
      301 gaaatcaagc ccaatagaaa accacaaatc atatagaatc ctatcttatc aaattgataa
      361 gtttttttaa aatctgttaa aacaaatgat acaagtagca tcacaaatag taaaatcatt
      421 tgaacaatgg atactagccg tactgataat tgagataaca aaacttgaaa aataagatga
      481 cctagaaacc ctcctaaaac agaagaaaag gataccgtaa aaatcaatct ccattttatc
      541 tctttacttt gtgaaaatct tttggttgtt gaaataatcg ccattataaa aacagagaaa
      601 ctagaataaa aagcaatatc actgaccgaa tggtatccaa aactatccat aagtggctta
      661 attataattc caccacccat gcctgatata gaacccaatc cactagctat tataatgatt
      721 agaaaataaa taatctcaac cactttttaa gtccatccta ttctttcaaa catctaaaac
      781 ctagatggct actagtggaa gtagaaatac atccatttct agcagctact cggtatcgat
      841 tacaataaga acaatgacat aaaaaggaac ctcctctgat agcatactcc tcatcttgta
      901 tcccatattt tggcaactca tagtcatctt cattaaaact agctaataag gtatatctcg
      961 gattgcgaca ccattcccaa acattcccaa tcatttgata tagaccaaaa tcattaggta
     1021 aaaattcata gacaggagcc gttcctataa atccatctaa ggcagtattt tcataaggaa
     1081 atcttccttg ccaagtattg gcatgataat atccaccttc taaaagagta tctccccaag
     1141 gatattcaga ttgtcttcct cctctagcag catattccca ctgtgcttca gtcggtaaag
     1201 acattccgct ccaattacag aaagccaatg catcttctaa agcaacatga actactggat
     1261 ggtcttctaa acccactaga ttactattct caccgtaggg atgtttccag caagcgttcg
     1321 gaacttgtaa ccaccacgga gccccagcag gatgaggata tccctccctt tctgcctctg
     1381 gaacgaacaa tataaaaaca aaagaccact cttgtcgctc agctagtgtt ttatagcctg
     1441 tttctttcac aaattgagca aattcttgat tcgtaactgg agtatccgca atggaaaaat
     1501 cctttacact aacagcgact tgaggacctt caaaatcatc taaaaagccc tcttcagaat
     1561 ttgttcccat cataaatgtc ccacccttga tttttttcat tttcatgatt gactatcccc
     1621 cgttttcgtt agagttggcg ttattttaga aagttcaaca ggaactaact cttcatccac
     1681 aacaaaacct tcttctctat atctaagaaa gtctactaat tttttcttca tctgacgaac
     1741 aataggttgg tatttttcac ttggatacaa atcattcatt tcatgcggat cttttttcat
     1801 atcaaataac tgataatgat ttaaaacagg aaaccagata aatttccact ggtctgtcaa
     1861 gatatattga ctagaatctt ttcctaaagc atgttcacca tgaaattctg ttcgccaccc
     1921 ctcgtactgt ccaaataata aattcttaac actccgtcca tctagttcat ctgtagtagt
     1981 tcctcctgcc aaatccacga gtgaaggaaa aatatcttga attttcacta attgcttgat
     2041 tgttccccta ttaccagcta tcaatccagc cggatcgtaa ataaacgaag gaatgtggat
     2101 acttccctga taaggatatc cctttctaaa cagataatgt tcaccaagct gatctccatg
     2161 atccgaaaca aaccagataa tcgtatcttt atcatgacga aattctttta gggcagtcaa
     2221 aaagcgacta atttgatggt ctatatgagt aatcaatcca aaataagcag caaccattct
     2281 tctttgatca tcttccttta atttccctct taaagcataa atagacggaa tttgcttttc
     2341 taatacttcc caattcccga tatgcaaatc gagaaattga ggcaagcgtt ccatatacat
     2401 atcaaaatag tattttgggg gatttaatgg agcatgtggt ttttcaaaag acattttgag
     2461 aaaaaaagga accgttggat ctcttctttg taaaaatgaa atggactcac tcacaaccca
     2521 gtttgttgga tgtaacttct catctttatc ccaaggtcta gcttcccaag aattacaatc
     2581 cattccgtca tcaatcaaat caacatcata accaacttta ccctttaaaa aggctagata
     2641 gtcactagca taatcaaatt gacttccata agccttatca tactttctat caacatgtag
     2701 atacccatcg tgaagtaaga catgatcaaa cccaagtctc tgtctggaag gaaaaacatg
     2761 catcttacca atacactctg tttgataacc catatcttta aagacttttg gaagagtatt
     2821 agtaaaattc caaggcacct catcttgata accaactcgt ccacttttat cttggtctaa
     2881 gccagtcaat aaagctgccc tagcaggaac gcaagaagga actggagaat aggcattttc
     2941 aaaattataa cctacactag ccatcatatc taatgttgga gtgctaacta gcttatcctt
     3001 actattcaag gataaggcgt ctgctctcat ttgatctaca acaatcaaaa taatatttgg
     3061 ctgttttgtc tgaaccataa aatctccttt ctaatatggc aaaagaggca caagaagata
     3121 tctaccttta ctgcacccct ttctatatca atctctctat ataaaacaat aacattcttg
     3181 ttatgtttta tagaaaaatg aactaaaata tgactaaatc gattaggaaa ttcaaatcat
     3241 tttctagtac tgttttagaa gttacagtgt actattccaa cttcaataaa ctataaacct
     3301 ttgtctaata acaattttag tggagataag aaatcctaca cctaactcat cttacacgta
     3361 atctatttct attttatcac aaaaaacgca agtaagacca ttaactcaat tcagttttat
     3421 atgccatttt cacaaatggg aaataagtca agacactaat aatcaaacaa acaactgata
     3481 agatgatggc acgccaatca aatgctgtag agaagaaacc atataaaatt ggaggcatta
     3541 cccaagtaac gttttgtgta acaggtgaaa caagacccca gcttgttgcc cagtaagcta
     3601 ccgttgccat gaaaaccgga ctaagtacaa atggtataaa tagcaaagga ttcaagacaa
     3661 ctggtaaacc ataattgacc ggctcaccaa tattaaacag aactggtgct agaccaagtt
     3721 tagcaacttt tcgataatga ctgtttcttg aaaaaattaa aatagcaagt actaatccta
     3781 atcctccaaa ccagacaaac gccccaaaag acccacttgt ccatatataa ggaatcggtt
     3841 cacctttttg gaaagcatcc agattcgcta acatagcaac tccaaacagc ccttccatga
     3901 tgggagccaa tacatttcct ccatggagac caaaaaacca gaataactta ttcaaaaaga
     3961 tcatcagaat aactgcaaag aaactttgag acaaacctag taatggcgtt tgtaacacct
     4021 tgtaaaccca atcaatcaat aagtcattgc taagtaaatg gaaaacataa gtcaagatgg
     4081 ctactatata catcgccata aatcctggaa tgatagaagt gaacggctta gcaatcgcag
     4141 ggggaactga atctggtaac ttgattaccc agttcttttt cattacttta cagaaaataa
     4201 tagaggctaa aaatccaatc atcatggctg taaagtagcc tctggcatta atatggtttc
     4261 ctggaatcac attcccaata gttaccatca gatttttacc atcaaatgct agattatcaa
     4321 ttccttgtaa gatttgatct aatttcacat ctcctacatt tgccaaaggg aaactctttg
     4381 taactgtact tccaatcgaa atgacaaacg aagcaagtga taccaaacca gcagaaactg
     4441 tatcaacctt gtaaatctta gcgatattca ctcccaagca atagatgaac aacaaggaaa
     4501 caattggtat acttcccttg aataccaaat tattgatgtc aacaagccac tgaaaggttt
     4561 tcgtaatact ttctaggtga aattgttgtg gtaaatccac tagaaaagca tttaataaca
     4621 aagcaatgga acctgtcata ataacaggca tagtccccac aaatgaatca cgtaaagcaa
     4681 ctaaaaactt ctgattccca actttcattg caattggaga aagtttttct tgcattttat
     4741 ccaacattgt attcatacca aactcctaat ttttttcaaa tcgttcgtat acttctacca
     4801 cttctttcgc caagtcaaca aaagtcagac tcgtcattaa atgatcttgc gcgtgtacca
     4861 tcaagaggct aattgatgtt ttttccccct gtgcctctct tgtcaacatt tctgtttgag
     4921 ccttatgcgc ctgtaataag gcagcattcg catcagctaa tcttcgattg gctttagaga
     4981 aatccccttt ttttgctgcc tgaatagcct ccatagcatt acttttggct tccccaccat
     5041 acataattag ccccattaca gattctaaat tactttcatc cataacattc tcctcaatct
     5101 tagtctaata aactttcagc taaatctaaa actttagaac cattcatcat tccgtaatct
     5161 gtcatcggaa taaccgccac aggaatctgt ctatctttta gtttttcttg aaagtcccct
     5221 agtaaatagc gaacttgagg acctaaaagc aacacattta cttcttttgt tgctacaatt
     5281 tcttctgctt caggagctgg aactgcaaaa atttctgcat ccaacccctt atcttctgct
     5341 gccttttgca tctttgtcac tagcagactt gtactcatac ctgctgcaca tgctaacata
     5401 attgttactt tagccatagc ttactccttt atcacctatt tactgaccta gccaagcgag
     5461 atactgtatg tctcactcct ctaattgcct tgctcaatgc ataaatatta tcgactgttg
     5521 ccagtccccc atatcctgcg tcacctatat gttgaatatc aactccacaa attttatttc
     5581 taagtgcaat ttccttgata gtatctgtat cagatgtttc ttggctagta ccaatagcac
     5641 tcaatacaag accaccctta ctatgaacga gatcaacgac ttcacgcaac tcttggtcat
     5701 gaaaggctgg aacggttcca actgctggaa caagtatcac atccgcacct gcttccaaca
     5761 attgctctgc gacagaaagc tctgccacag gctcattcac tcctgccccg tgcatcttac
     5821 ctgcaataat caaaccagaa aagttttcct tagcagtttg aacagcctta atgatttctc
     5881 gattgctaac tcctgttcca ggatttccag tcaaacagac aaagtcaaaa cctaactcct
     5941 ctattcgatt caatgtttca acactagcaa cacgacctgc aacaatttcc tgtgtttcct
     6001 ctagcatctt tgcagatggg tcaatcggtt ccaaatttac accaattgga caagcaacaa
     6061 gctggtgtaa gcgatgaata acatcatcac ctgaactatc caaagcataa attttaggct
     6121 caaaaacatc aacacaattc aataaaatca tatcagctcc aaaagctcta gcaatttcag
     6181 aattagttat atccccaaca aatgtctcac gagtaactac attttcagat agaacaactc
     6241 taccttcact tgctaaaata ctttgtttta attcttcagc attcatctgt aatatctcag
     6301 atggatttgc actaattaaa cgtttcattc tcaatcctcc aataacttaa atcgtttttc
     6361 aatagctatt acaaatggta aataaagtaa gatatccaaa acaatcaata caatttgtag
     6421 taatgaacca gaaatacttc ctgtagcaag taaaccactt ataacaggtg gcatagtcca
     6481 acctggatct gtataagtta gcggaaccaa acctgatgcc attgctcccc acgctactag
     6541 taagttaaac attggagcta agataaatgg aactagtaac aaaatgttca aaacaatagg
     6601 aacgccaaac attgctggtt catttatatt aaataatcca ggtataacag ttataggtgc
     6661 caaagttttt agttgtttac tagctttctt cttatgtgct aaatatccta acgcaagaac
     6721 taaacctatg gttgcaccac cgccacctat gaatacaaaa ttatccataa atacgctagt
     6781 aaaaatattt ggaagagttt ctccagtttg ataagctact ttatttgcat ctagattagc
     6841 taaccataac ggtttcataa ctgcattaac aacatttcct ccgtgtaatc ctacaaacca
     6901 aaaactacta ttaaggaaaa ttaagataag aagaccaata acattatttc ctaaaagtcc
     6961 aagtggacct cccaaagcta cttgagcaat ttcatgaaga ttaggtaaac ttaatttatc
     7021 taaagttgca taaacaatca accaaccaac aatagtcact gcaccaggaa taatggcact
     7081 aaagctttta gatactgctg gtggaacacc atccggcatt tttatctgta tattatgatt
     7141 tataaaccat tggtagatat agccattaat caatcctaaa atcatagcaa caaataaacc
     7201 ttttgatgcc atgaaagcgg taggcattcc tgctccagcc tctcctctaa tgaaaggcgt
     7261 aactgtaata aaagatgata atgacacaat accagcagga ataccatcaa ctttgtattg
     7321 tctagccata aagtaagcga taccaaaact agctacaaga ccaagtaatc caaaactact
     7381 atcaacaccc ttccataaat agtcagcaac accaatatca cccagatatt tttcccatcc
     7441 tggaatagga aaacttgcaa taaccattag taatgaacct atcaacaata aaggtatagt
     7501 tagagtaata ccgtctcgaa tagaaaccaa cgctttatta ttacccaatc tagccgctac
     7561 tggaagtaac ttttcttgta ataatttttc cataaccttt tcctcttaaa tatattttat
     7621 tatcaaaagt tatcaattat ctttattttt tgacgtcaag ttgttatttg attatctttt
     7681 gttatttata ttttacaact ataagaaagc c
//
LOCUS       DAVLXF010000087         7613 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLXF010000087 DAVLXF010000000
VERSION     DAVLXF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.087"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            433..2538
                     /locus_tag="VYO12_001781"
     CDS             433..2538
                     /locus_tag="VYO12_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1588334.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMSQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFNILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGNFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(2834..3316)
                     /locus_tag="VYO12_001782"
     CDS             complement(2834..3316)
                     /locus_tag="VYO12_001782"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1588335.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(3411..4895)
                     /locus_tag="VYO12_001783"
     CDS             complement(3411..4895)
                     /locus_tag="VYO12_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1588336.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPGGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMSERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            5048..6655
                     /locus_tag="VYO12_001784"
     CDS             5048..6655
                     /locus_tag="VYO12_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1588337.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKELLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            6820..6987
                     /locus_tag="VYO12_001785"
     CDS             6820..6987
                     /locus_tag="VYO12_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV1588338.1"
                     /translation="MKKRILSAGLTFTSDLLLAACGQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(6980..7613)
                     /locus_tag="VYO12_001786"
                     /pseudo
     CDS             complement(6980..7613)
                     /locus_tag="VYO12_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2291 a         1431 c         1719 g         2172 t
ORIGIN      
        1 tctaaaacat tgttagaaat cgatttgact gtcctgaacg atttatcctg ttcttatttc
       61 attttactat acagtttcga ggttgtagat aaggcgaagc tgatgtggtt tgaagagatt
      121 ttcgaagaga taaaaaatat tggtcaatta aagtcaaaat ccttgacctt ttctgactaa
      181 tgtagtatat tatgaacgta aggtaaatga aagccttact agaacactta tttaaagtaa
      241 aatagaaaga ggtggggtct atgtttaatc tagaaatctt cagaagtaaa gatggtctac
      301 tcctgcttga aaaagaaaaa ccagaaatag tacgtagggt agcggtttag ctagtctaaa
      361 ttttttaaaa caaaggtcaa agatagtcaa tatcagtaat cataactaag taaacaaaaa
      421 gaggtaaaga atatgaataa caactttaat aattttaata acatggatga tttatttaac
      481 caattgatgg gtggtatgcg aggatacagt tctgaaaatc gccgttactt aattaatggg
      541 cgcgaagtca cacctgagga atttgctcac tatcgtacga ctggtcaatt accaggaaat
      601 gcagaaactg atgtgcaaat gtcacaacag gcatcaggta tgaaacaaga cggtgtcctt
      661 gcaaaactag gtcgaaactt gacagcagaa gcgcgtgagg gtaagttgga tcctgttatc
      721 ggacgaaaca aggaaattca agaaacatct gaaatcctct cacgccgcac caagaacaat
      781 cctgttttgg tcggagatgc aggtgttggt aagacagcag ttgtcgaagg tctagcgcaa
      841 gccattgtga acggagatgt tcctgctgct atcaagaaca aggaaattat ttctattgat
      901 atctcaggtc ttgaggctgg tacccaatac cgtggtagct ttgaagaaaa tgtccaaaac
      961 ttagtcaatg aagtgaaaga agcagggaat attatcctct tctttgatga aattcaccaa
     1021 attcttggtg ctggtagcac tggtggagac agtggttcta aaggacttgc ggatattctc
     1081 aagccagctc tctctcgtgg agaattgaca gtgattgggg caacaactca agacgaatac
     1141 cgtaacacca tcttgaagaa tgctgctctt gctcgtcgtt tcaacgaagt gaaggtcaat
     1201 gctccttcag cagagaatac ttttaacatt cttcaaggca ttcgtgacct ctatcaacaa
     1261 catcacaatg tcatcttgcc agacgaagtc ttgaaagcag cggtggatta ttctgttcaa
     1321 tacattcctc aacgtagctt gccagataag gctattgacc ttgtcgatgt aacagctgct
     1381 cacttggcgg ctcaacatcc agtaacagat gtgcatgctg ttgaacgaga aatcgaaacg
     1441 gaaaaagaca agcaagaaaa agcagttgaa gcagaagatt ttgaagcagc tctaaactat
     1501 aaaacacgca ttgcagaatt ggaaaagaaa atcgaaaacc acacagaaga tatgaaagtg
     1561 actgcaagtg tcaacgatgt ggctgaatct gtggaacgaa tgacaggtat cccagtatcg
     1621 caaatgggag cttcagatat cgaacgtttg aaagatatgg ctcatcgctt gcaagacaag
     1681 gtgattggtc aagataaggc cgtagaagtt gtagctcgtg ctatccgtcg taaccgtgct
     1741 ggttttgatg aaggaaatcg cccaatcggc aacttcctct ttgtagggtc tactggggtt
     1801 ggtaagacgg agcttgctaa gcaattggca ctcgatatgt ttggaaccca ggatgcgatt
     1861 atccgtttgg atatgtctga atacagtgac cgcacagctg tttctaagct aattggtaca
     1921 acagcaggct atgtgggtta tgatgacaat agcaatacct taacagaacg tgttcgtcgc
     1981 aatccatact ctatcattct cttggatgaa attgaaaagg ctgaccctca agttattacc
     2041 cttctcctcc aagttctaga tgatggtcgt ttgacagatg gtcaaggaaa tacagtaaac
     2101 ttcaagaaca ctgtcattat tgcgacctca aatgctggat ttggctatga agccaacttg
     2161 acagaagatg cggataaacc agaattgatg gaccgtttga aacccttctt ccgtccagaa
     2221 ttcctcaacc gctttaatgc agtcatcgag ttctcacact tgactaagga agacctttct
     2281 aagattgtag atttgatgtt ggctgaagtt aaccaaacct tggctaagaa agacattgac
     2341 ttggtagtca gtcaagcggc taaagattat atcacagaag aaggttacga cgaagtcatg
     2401 ggggttcgtc ctctccgtcg agtggttgaa caagaaattc gtgataaggt gacagacttc
     2461 cacttagatc atttagatgc taaacatctg gaagcagata tggaagatgg cgttttggtt
     2521 attcgtgaga aagcctaaga tagaattttg aggataaaaa agaaggagcc agctgaaaaa
     2581 aactggttcc ttttgaggta cgacgggcat gtcgtacagt agaagtgtac tattctagtt
     2641 tcaatccact atagtagctc agaagtcggt acttaaacgt gctatatcaa aaccagtcct
     2701 tgaaaaacgt ggattggttt cgtgtttgga ttattacctt gaacgacatg cgttaaaagt
     2761 tagttgaacc gccgtatgcc gaacggcacg tacggtggtg tgagaggggc tagagattat
     2821 cccctactcg attttaaatc acatgacgtt caaaggcatc atctgaaatc ccttgttcca
     2881 agatgagttt tgcccattct ttagcagaga agaggctgtg gtccttgtag tttccgcaag
     2941 attcgatggt tgtccctggg acatcttccc aagtagtagt ttcagcgatt tccttgagcg
     3001 aatccttgat aacagctgcg attttagcac tggtgtgacg tccccacata atcatgtgga
     3061 agcctgtgcg gcaaccaaat ggtgaacagt caatcatgcc gtcaatgcgg gtacggatga
     3121 gtttggctaa gaggtgctcg atagtgtgaa ggccggcagt agggatagag tcttcgtttg
     3181 gttgcaccaa gcgaatatca taattggaga tgatgtctcc ttttggtcct gtttcttccc
     3241 caatcaagcg aacatagggt gctttgacaa tggtgtggtc aagttcaaaa ctttcgacaa
     3301 taacttcttt tgacatggta aatcctttca gttttcttct ctcattatat cataaaggtt
     3361 gttcctgaga cagagagaaa acctctccga ggctagagag gttgaaatct ttacttacga
     3421 tataagcggt cgtattggta gtatgggtca aaggttacgt tgatacccag tttacgaagg
     3481 acattcttgt cttcatcagt caagatgatg gttgagtggg cttcgcttcc tttgaggttg
     3541 cctagttctt ccatagcgcg ggcagcatca ggattttctg tagctgtgat agcaagtgca
     3601 atcaggattt catttgaatg aaggcgtgga ttgcggctac cgagatgatc gattttaaga
     3661 ccttggattg gcttaacaac ttcaggttcg attagtttta cttctttagc gatgtcagct
     3721 gattttttga tggcgttgat caaggcagcg gctgtaggac caaagagttc tgagttctta
     3781 ccagtgacga tttccccatt tggcaattca aaggctaggg ctggtccacc agtttcttct
     3841 gctttttggc gcgcaacgac agcaacctta cggtctgcag gtgtgatacc gaggtcgttc
     3901 atgagcaact caattttctt gacggcagct tcgccaactt tttcagcttt gaagtcaaga
     3961 actgtttgat agtaacggcg gatgatttct tgtttagaag cttcgacagc ggcctcgtca
     4021 tctgtaatag cgaaaccaac catgttgaca cccatatctg tcggtgaagc gtatggtgat
     4081 tttccgagaa tacgttccga catgcgtttg agcactggga agatttcgat atcacggttg
     4141 tagttgacag tggtttctcc ataggtttga agatggaagg ggtcaatcat gttgacatca
     4201 tcaaggtcag ctgtggcagc ttcataagcc aagttaactg gatgatgaag gggaagattc
     4261 caaacaggga aggtttcaaa tttagcgtag ccagatttga tgccattgat ttggtcgtgg
     4321 tacatattgg acatacacgt tgccaatttt ccagaaccgg gtccaggagc ggttacgaca
     4381 atcaagttgc gactggtttt gatgtagtcg tttttgccca tgcctcctgg ggaaatgatg
     4441 tgatccatat ccgtaggata tcctttgatt ggataatgaa gataagaatc aattccgttt
     4501 ttctcaagtt ggttgcggaa ggcatctgca gcgggttggc cagcgtattg tgtaatgaca
     4561 acggaaccaa caaaaatccc taattcattg aatttgtcaa tcaaacgaag aacttcttgg
     4621 tcataagaaa tgcctaagtc gccacgtgct ttggaatgtt caatgttgct agcattaatg
     4681 gcaatcacaa cctcaacctg ctctttcaat tcttgcaaga gcttgatttt gttgtcaggt
     4741 tcataaccag gaaggacacg agcagcgtgg aaatcttcta acattttacc gccaaactct
     4801 aagtagagct tgccgtcaaa ttggttaatg cgctccaaaa tatggtcgcg ttgtaaattc
     4861 aaatactgtt cagaactaaa agcttgtttt ttcatttttt tacctctgga ctctattata
     4921 ataaaaaatt ggaagttagg aaactacgga gctaaaaaag aaattaaaaa gattaagcaa
     4981 acgcttgcac aaaattttaa aaagtgctat catagactat agattattaa aataatgagg
     5041 taaacagatg caagaaaaat ggtggcataa tgccgtagtc tatcaagtct atccaaagag
     5101 ttttatggat agtaatggag atggagttgg tgatttgcca ggtattacca gtaagttgga
     5161 ctatctagct aagttaggaa tcacagcgat ttggctttct cccgtttatg acagccctat
     5221 ggatgataat ggctacgata ttgctgatta tcaagcgatt gcggctattt ttggaaccat
     5281 ggaggacatg gatcagctga ttgcagaagc taagaagcgt gatatccgta tcatcatgga
     5341 cttggtggtc aatcatacct cggatgagca tgcttggttt gtcgaagcct gtgaaaatac
     5401 tgacagccct gagcgagact actatatctg gcgcgatgaa cccaatgacc tagattctat
     5461 ctttagtggg tctgcttggg aatacgatga aaagtcaggt caatactatc tccacttttt
     5521 cagcaagaaa cagccggatc tcaactggga aaatgaaaaa cttcgccaga aaatttatga
     5581 gatgatgaac ttctggattg ataaaggtat tggtggtttc cgtatggatg ttattgacat
     5641 gattggcaaa attcctgacg agaaggtagt caataatggt cctatgctcc atccctatct
     5701 caaggaaatg aatcaggcga cctttggaga taaggagctc ttgacagtag gggagacttg
     5761 gggagcaacg ccagagattg ccaagctcta ctctgatcca aaggggcaag aattgtctat
     5821 ggtcttccag tttgaacata tcggtcttca gtatcaggaa ggtcagccta aatggcacta
     5881 tcaaaaagag ctgaatatcg ctaagttaaa agaaatcttc aacaaatggc agacagagtt
     5941 aggagttgag gacggctgga attccctctt ctggaacaac catgacctcc ctcgtattgt
     6001 ctcaatctgg ggaaatgacc aagaataccg cgaaaaatct gccaaagcct ttgcaatctt
     6061 acttcatctc atgagaggaa ctccttatat ctaccaaggt gaggagattg ggatgaccaa
     6121 ctatccgttt gaaacactgg atcaagtaga agatattgaa tccctcaact atgcgcgtga
     6181 ggctcttgaa aaaggtgttc cgattgaaga aatcatggac agtatccgtg ttattggacg
     6241 tgacaatgcc cgtaccccta tgcaatggga cgagagcaaa aacgctggtt tctcaacagg
     6301 tcaaccttgg ttggcggtta atccaaatta cgagatgatc aacgtccaag aagcgctggc
     6361 aaatccagat tctattttct atacctatca gaaactggtc caaattcgca aggagaatag
     6421 ctggctaatt cgagctgact ttgaattgct tgatacggct gataaggtct ttgcttatat
     6481 acgtaaggat ggcgaccgtc gcttcctagt tgtggctaac ttgtctaatg aagagcaaga
     6541 cttgacagta gaaggaaaag tcaaatctgt cttgattgaa aacaccctag ctcaagaagt
     6601 ctttgaaaaa caaatcttag ttccatggga tgctttctgt gtggaattac tataaatatt
     6661 ttttgcagaa aaatttaaaa ttgaaatcgt ataaaaacaa gggaggactg tataaaaggc
     6721 agaaatcctt tgttttttat aaccaaggtt tataaacctt cattctcgaa attcaattaa
     6781 ctttacaaat tcccactatt aaggagaaag aagacgaaca taaagaagcg tatccttagt
     6841 gcaggcctga cttttacatc tgatttgctt ttagctgctt gcggccaatc aggttcagat
     6901 acaaaaactt actcatcaac ctttagtgga aatccaacta catttaacta tctattagac
     6961 tattacgctg ataatatagt caattgaaac aagaacagga caaaagagcc tcataaaagg
     7021 tattgcaact tggtaatacc tttttgaggt gctttttgat atgagcccat gttttctcaa
     7081 taggattgta ctcaggcgag tagggaggaa gaggtaaaag tttatgccca aactcttcac
     7141 acaagagttc tagcttcccc attctatgga atcttgcatt atccataata ataaccgatg
     7201 gtgtggttaa tgttggtaag agaaacttct gaaaccaagc ttcaaaaaag tcgctcgtca
     7261 tcatctcttc gtaagtcatt ggagcgatta actcaccatt catttgttag acctgcaacc
     7321 aaagaaatcc tctgatatct tcttccagat actttacctc ttcttaactg accttttaat
     7381 gagcgaccat attctcgatg aaaataagta tcgaatcctg tttcatcaat ctaaacaggt
     7441 gctaggtgct ttaaactatt aaaattctta agaaataagg ctactttttc tgggtcttgt
     7501 tcatagtagg tgtggttctt tttttcgagt gtagcccata gctttgagcg catagtggat
     7561 ggtagttgga tgacaggcaa attcagaagc tatttcagtc aaataagcgt ctg
//
LOCUS       DAVLXF010000088         7588 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLXF010000088 DAVLXF010000000
VERSION     DAVLXF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.088"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            71..370
                     /locus_tag="VYO12_001787"
                     /pseudo
     CDS             71..370
                     /locus_tag="VYO12_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            441..2462
                     /locus_tag="VYO12_001788"
     CDS             441..2462
                     /locus_tag="VYO12_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1588339.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2464..3105
                     /locus_tag="VYO12_001789"
     CDS             2464..3105
                     /locus_tag="VYO12_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588340.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3209..4561
                     /locus_tag="VYO12_001790"
     CDS             3209..4561
                     /locus_tag="VYO12_001790"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1588341.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATD
                     KNTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4565..5623
                     /locus_tag="VYO12_001791"
     CDS             4565..5623
                     /locus_tag="VYO12_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1588342.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5633..6832
                     /gene="divIB"
                     /locus_tag="VYO12_001792"
     CDS             5633..6832
                     /gene="divIB"
                     /locus_tag="VYO12_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1588343.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     NQRSSRR"
BASE COUNT         2348 a         1304 c         1664 g         2272 t
ORIGIN      
        1 aaaagagaga ggaggattta ttttgttgca ttgttgctca aggtgtgctt tttttgaaag
       61 aaaaaataaa atgaaaacga aaaaacatag attacttgct ctagctctta tttcaaattt
      121 tacattattg ggagctgcat cagctgctgt acaatatcca gatggaggag tatggacata
      181 tggagaaggt tcaggaggtg gttaggcttt ttcaaattac tatcatggta aaaaatatca
      241 ttattcatcc atcgtgagca gatgggatgg tcattctgat aaaggagaag ctcctgctgg
      301 aaaaacctcc tatgcatgga tttggactaa atggggagaa caagtagcat tttactgtga
      361 ttatgactaa tcgttcattg atttaaatcg ttccccttgt aagaaattac agggggaatt
      421 ttgtttacaa ggagtctact atgaagaaac tattcatatt attatcaact ttttttctca
      481 gcttcttcct tgcttggatt attgtcttac gtgcgccaca atatttatat gcaagctatg
      541 attccgtttc tttacttcgt gtcaaaaaag atactcagga accgacgcgt gaggtatttg
      601 aacaggaatt ggagaatttt gcaaactcag aacagagttt aatagctaga agaattgtag
      661 agccgagtaa ggatgggacg actcacttta cttatgcaac ttatggtcag ggaactttac
      721 caaaagaatt ccaagaagct agccaagaaa gtcgtgaacg tagtgatccg ctaaatagtt
      781 atctcctttt gtcaggctcc ttgacgaaag aaaagcttgc cgataaatta ggagatttgg
      841 gttataaagc aagtgctgac cgaaagatac cgccctattt tcttgctttt cgaatattac
      901 taaatcccct tattttaatt agtttagcaa tatttggctt atctttcttt gctttagtga
      961 ttatcactcg gattaaggaa atgagagcag caggtataaa actcttttct ggtcagactc
     1021 tcttatccat catggggcat tctttatcta ctgatatcaa atggctcctt ctatcagccc
     1081 tcctttcctt cctaggtggg ggagtcgttc tttttagtca aggtttgttt tatcctatct
     1141 tgttagccac ctatggtttt gggattagtt tctatctgtt gtttttattg gcgatttcaa
     1201 ttttactaat gcttctttat ctaatgagtt tgaattacaa agcattagtt cccgttatta
     1261 aggggagatt accccttaaa cgcctgatga ttttaaccct attgtgtcag ttggttgctg
     1321 tctttacagt aggatacgct gttaagacag gtttgacgtc ttaccaacga ttgaaagaac
     1381 ttgaaatttc aaaacaagca tggcaggata gagcagacta ttatcaaatt tcttttggct
     1441 taggtgatag aggaaaagat acagaaaatc agagcaagtg gtatgccttt gccaaggaag
     1501 caatcgaaga agaacaagct ctttatgtaa aggataatct gctccatttt gccaatccac
     1561 aaggaaaaaa tgaacaggga gagacactgg atacctatag tccagatgct aatacgctct
     1621 atgttagtcc cagttatttg gacaaggaaa aggtcgtggt agatgctgag accaaacaga
     1681 agttagccca tctccaaaaa ggtgagttta tcctcttgct cccagaacat ttgcgctctc
     1741 gagaagcaga acttaagaaa gtttttgaag aaagattgag ttattatgga aaatctggtg
     1801 aggaggcaag tgctcctttg gattatgaga tgaaagcgca cgttagttat ctttcaatgg
     1861 gagaaaagcg gtttgtttat aataacggtg agaatcccgt atccactcag tatttgactg
     1921 atccgatttt agtggtattc acgccgactt ctacaggtga tagttttata tccctatcta
     1981 gttggtctat caatgctgga aaacaactct ttatcaaagg atatgagagt gggctagaac
     2041 ttttgaagaa agctggaatt tatgagcaag tatcctatct taaagaagga agaagtgttt
     2101 atctaactcg ttataatgaa gttcaaactg aaacagcaac tttaatctta ggagctattg
     2161 tggggatagc tagttccttg ttactatttt attctgtcaa tcttctatat ttcgagcaat
     2221 tccgccgaga tatcttgatt aaacgaattt caggtttacg attttttgaa acacatgctc
     2281 agtatatggt tagtcaattt gccagttttg tatttggtgc tagtcttttt attttaagca
     2341 gtcgagactt ggtgattggc ttgctcactt tattagtctt tctagctagt gcagttctga
     2401 cgctttaccg tcaagcgcag aaagaatctc gtgtttctat gacaattatg aaaggaaaat
     2461 aggatgattg aactaaagaa tatatctaaa aaatttggaa gccgtcagct attttcagat
     2521 acgaatcttc attttgaagg tgggaaaatt tatgccttaa tcggtacaag tggctgtggt
     2581 aagacaacac tcttgaatat gattggacga ttagagccat atgacaaagg gcaaatcatc
     2641 tatgatggca cttctcttaa ggacatcaag ccttctgttt tctttagaga ttacttagga
     2701 tacttatttc aagattttgg cttaattgaa agccaaaccg tcaaagagaa tctcaatctg
     2761 ggtttagttg gtaaaaagtt gaaggaaaaa gagaaaatct ctttgatgaa acaagctcta
     2821 aaccgtgtaa acctctctta tttggatttg aagcaaccta tatttgagtt atcaggagga
     2881 gaagcacaac gtgttgcatt agcgaagata attttaaagg atccaccttt gatcctcgca
     2941 gatgaaccaa ccgcttcctt agaccccaaa aactctgagg aattactttc catcctagaa
     3001 tctttaaaaa atccgaatcg aactattatt attgcgaccc acaatcctct tatttgggaa
     3061 caagtggacc aagttattcg agttaccgat ttatcacata gatgatatgg caagattcag
     3121 ctagaagaaa gagtcacaaa cacactttgt ggctttttta tttccataaa aatggtaaaa
     3181 tagtaggagt agaaatggag ttcgagacat gaaagtaata gatcaattta aaaataagaa
     3241 agtccttgtt ttaggtttgg ccaagtctgg tgaatctgca gctcgtttgt tggacaagct
     3301 aggtgccatt gtgacagtaa atgatgggaa gcctttcgag gacaatccag ctgcccaaag
     3361 tttgctggaa gaagggatca aggtcattac aggtggccat cctttggaac tcttggatga
     3421 agagtttgct cttatggtga aaaatccagg tatcccctac aacaatccca tgattgaaaa
     3481 ggctttggcc aagggaattc cagtcttgac tgaggtggaa ttggcttatt tgatttcaga
     3541 agcaccgatt attggtatca caggatcgaa cggtaagaca accacaacga ctatgattgg
     3601 ggaagttttg actgctgctg ggcaacatgg tcttttatca gggaatatcg gctatccagc
     3661 tagtcaggtt gctcaaatag cgacagacaa gaacacgctt gttatggaac tttcttcttt
     3721 ccaactcatg ggtgttcaag aattccatcc tgagattgcg gttattacta accttatgcc
     3781 aactcatatc gattatcatg gattatttga ggaatatgta gcagccaagt ggaatatcca
     3841 gaacaagatg acagcagctg atttccttgt cttgaacttt aatcaagact tggcaaaaga
     3901 cttggcttcc aagacagaag ccactgttgt accattttca acacttgaaa aggttgatgg
     3961 agcttatctg gaagatggtc aactctactt ccgtggtgaa gtagtcatgg cagcgaatga
     4021 aatcggtgtt ccaggtagcc acaatgtgga aaatgccctt gcgactattg ctgtagccaa
     4081 gcttcgtgat gtggacaatc aaaccatcaa ggaaactctt tcagccttcg gtggtgtcaa
     4141 acaccgtctc cagtttgtgg atgacatcaa gggtgttaaa ttctataacg acagtaaatc
     4201 aactaatatc ttggctactc aaaaagcctt atcaggattt gacaacagca aggtcgtctt
     4261 gattgcaggt ggtttggacc gtggcaatga gtttgacgaa ttggtgccag acattactgg
     4321 actcaagaag atggtcatcc tgggtcaatc tgcagaacgt gtcaaacggg cagcagacaa
     4381 ggctggtgtc gcttatgtgg aggcgacaga tattgcagat gcgacccgta aggcctatga
     4441 acttgcgact caaggagatg tggttcttct tagtcctgcc aatgccagct gggatatgta
     4501 tgctaacttt gaagtacgtg gcgacctctt tatcgacaca gtagcggagt taaaagaata
     4561 aaatatgaaa aaaattgtct ttacaggtgg ggggacggtt ggacacgtta ccctcaatct
     4621 tttgttaatg cccaagttca tcgaagatgg ttgggaagtc cactatatcg gggacaagcg
     4681 tggtatcgaa caccaagaaa tccttaagtc aggtttggat gtcaccttcc attctattgc
     4741 gactgggaaa ttgcgtcgct atttctcttg gcaaaatatg ctggacgtct tcaaagttgg
     4801 ctggggaatc gtccaatcgc tctttatcat gttgcgactt cgtccacaga cccttttttc
     4861 aaagggaggc tttgtctcag taccgcctgt tatcgctgcg cgtgtgtcag gagtgcctgt
     4921 ctttattcac gaatctgacc tgtctatggg cttggccaat aaaatcgcct ataaatttgc
     4981 gactaagatg tattcaacct ttgaacaagc ttcgagtttg gctaaggtcg agcatgtggg
     5041 agcggtgacc aaggtttcgg atcaaaaaaa tccagaacca gatgaattgg tggatattca
     5101 aagccacttt aatcataaat tgccgactgt attgtttgtt ggcggttctg caggtgctcg
     5161 tgtctttaac caattggtga cagaccataa gaaagaacta acagagcgct acaatattat
     5221 caatctaact ggagattcta gcctgaatga gttgagccaa aatctttttc gtgttgacta
     5281 tgtgaccgat ctctatcaac ccttgatgga attggctgat attgttgtga cacgtggtgg
     5341 tgccaatacg atttttgagc tcttggcgat agcaaaattg catgtcattg tgccacttgg
     5401 tcgtgaagct agtcgtggtg accagattga aaatgcagct tactttgtta agaaaggcta
     5461 tgcagaagac cttcaagaaa gcgatttgac cttggatagt ttggaagaga agctttctca
     5521 cttactaagt cacaaggaag attaccaagc taagatgaaa gcttctaagg aattgaaatc
     5581 tctagcagat ttttatcaat tgttgaaaaa agatttatca taaggaaagt aaatgtcaaa
     5641 agataagaaa aatgaggaca aagaaatcct cgaagaattg aaagagttat cagaatggca
     5701 gaaacgaaac caagaatatc taaaaaagaa ggctgaagaa gaagcggctc tagctgagga
     5761 gaaggaaaag gaaagacaag ctcggatggg agaagaatct gagaagtcag aggacaaaca
     5821 ggaccaggag agtgaaacag accaggaaga ttcagaatca gctaaggaag agtctgaaga
     5881 aaaagtagca tcctcagagg ctgacaaaga gaaagaagag aaagaagaat cagagtctaa
     5941 agagaaggag gaacaggata aaaagcttgc taaaaaggct acaaaggaaa aaccagccaa
     6001 agcaaagatt cctggtatcc atatcttgcg agccttcacg attttatttc caagtctgct
     6061 tttattgatt gtctctgcct acttgctcag tccttatgcg accatgaaag atattcgtgt
     6121 tgagggaacg gtgcaaacta cagctgatga tattcgacag gcttcaggca ttcaggattc
     6181 ggattatacg attaaccttc tgctagacaa ggcaaaatat gaaaagcaga ttaagtctaa
     6241 ctattgggtt gaatcagctc aacttgtcta tcaatttcca actaagttca ctattaaggt
     6301 caaggaatat gatattgtgg cctactatat ttctggtgaa aatcattatc ctattctttc
     6361 cagtggtcag cttgagacta gttctgtgag tctgaacagt ttaccagaaa cttatttatc
     6421 agttctcttt aatgatagtg aacaaatcaa ggtttttgtc tcagaacttg ctcaaattag
     6481 cccagaactc aaggcggcta tccaaaaggt ggaattagcc ccaagcaagg tgacatccga
     6541 tttaattcga ttgaccatga atgattcgga cgaagtcttg gttcctctat ctgaaatgag
     6601 taagaaactg ccatattaca gtaagattaa gccacaattg tcagaaccga gtgtggtcga
     6661 catggaagct ggaatttaca gttacactgt ggcggataaa ttaattatgg aggctgagga
     6721 aaaagccaaa caagaggcca aggaagctga gaaaaaacaa gaagaagaac agaaaaaaca
     6781 agaggaagag agcaatcgaa atcaaacaaa tcagcgttca tcgcgtcgct aggtttacct
     6841 tttctcttat agttctttag tgaccatgtt tttacgttta atatttgaca tttgtttttc
     6901 tttatgttac atctgcaatg taatcgatta caaaataatt tttgatgaag aaggtaacac
     6961 atatgtcaga tgaagtattc ttatttgaag aaattgaaga aatcgtagcc cctacagatg
     7021 gtgaattttt gggggaagtt ttacttggaa ctggggtagt tctcttaatt ggagtagcct
     7081 gttgttaaaa agatagggag tgataatcat gcaagataac tttttatttg aggaaattga
     7141 agaaatttca gtaccagtta gtgatttttc agctggactt gcaacaggta tcggatttgg
     7201 tttagcaatc cttgctcttg ctggttgttg aagtttgttc atttactaac atcaagcttt
     7261 ttcaatttca ttttagacag tcatttgaat tttccgtatt agtcttgcag caagagatta
     7321 atagaattag tcattatttt attgattgcg gactgaggga ctagagtatg ttttacttag
     7381 cccctctttt atttattaaa ggttaggttt gttatgagaa ttgttgataa gattaagata
     7441 ttacctactc cttatgaggg acagtatcat ctatatatac catccagtaa gaaacatgta
     7501 ttagttggga aacaggaaaa aaatggttag agcaactaat aggtcaagaa tttaccatat
     7561 cggacttatt agtgttagta gggaagaa
//
LOCUS       DAVLXF010000089         7567 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLXF010000089 DAVLXF010000000
VERSION     DAVLXF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.089"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..775
                     /locus_tag="VYO12_001793"
                     /pseudo
     CDS             1..775
                     /locus_tag="VYO12_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            836..1798
                     /locus_tag="VYO12_001794"
     CDS             836..1798
                     /locus_tag="VYO12_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1588344.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYEDTSISVSSNETDKV
                     LAGSLYTDTNEQGLMIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAEASKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1975..2982
                     /gene="gap"
                     /locus_tag="VYO12_001795"
     CDS             1975..2982
                     /gene="gap"
                     /locus_tag="VYO12_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1588345.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(3050..3886)
                     /locus_tag="VYO12_001796"
     CDS             complement(3050..3886)
                     /locus_tag="VYO12_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1588346.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            4012..6207
                     /locus_tag="VYO12_001797"
     CDS             4012..6207
                     /locus_tag="VYO12_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1588347.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            6260..6412
                     /gene="rpmG"
                     /locus_tag="VYO12_001798"
     CDS             6260..6412
                     /gene="rpmG"
                     /locus_tag="VYO12_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1588348.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6422..6598
                     /gene="secE"
                     /locus_tag="VYO12_001799"
     CDS             6422..6598
                     /gene="secE"
                     /locus_tag="VYO12_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1588349.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6653..7189
                     /gene="nusG"
                     /locus_tag="VYO12_001800"
     CDS             6653..7189
                     /gene="nusG"
                     /locus_tag="VYO12_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1588350.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
     gene            7311..>7567
                     /locus_tag="VYO12_001801"
     CDS             7311..>7567
                     /locus_tag="VYO12_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1588351.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRKYDKEP"
BASE COUNT         2394 a         1320 c         1626 g         2227 t
ORIGIN      
        1 tcacacgttt tccaaatctc acgtaatacc atttatggct ggttaaagct aaaagagaaa
       61 acaggagagc taaaccacca agtaaaagga acaaaaccaa gaaaagttga tagagataga
      121 cttaaaaact atcttactga caatccagat gcttatttga ctgaaatagc ttctgaattt
      181 ggctgtcatc caactaccat ccactatgcg ctcaaagcta tgggctacac tcgaaaaaaa
      241 gaaccacacc tactatgaac aagacccaga aaaagtagcc ttatttctta agaattttaa
      301 tagtttaaag cacctagcac ctgtttagat tgacgaaaca ggattcgata cttattttta
      361 tcgagaatat ggtcgctcat taaaaggtca gttaataaga ggcaaagtat ctggaagaag
      421 atatcagagg atttctttgg ttgcaggtct aacaaatggt gaattaatcg ctccaatgac
      481 ttacgaagag acgatgacga gcgacttttt tgaagcttgg tttcagaatt ttctcttacc
      541 aacattaacc acaccatcgg ttattattat ggataatgta agattccata gaatggggaa
      601 gctagaactt ttatgcgaag agtttgggca taaactgtta cctcttcctc cctactcgcc
      661 tgagtacaat cctattgaga aaacatgggc tcatatcaaa aagcacctca aaaaggtatt
      721 accaagttgc aatacctttt acgaggctct tttgtcttgt tcttgtttca attgactata
      781 gttcacggat acagttggga aagaagctat taattatttg gagagtcctt ttgggatgaa
      841 catgtcaaat attactatag atgggcaaga aatggatctg agtcaaacga tgccttctta
      901 tgctcaagta gtgagtcttt atgaggatac aagcatctct gttagtagta atgagacgga
      961 caaagtgttg gctggatccc tctatactga tacaaatgaa cagggattaa tgattccaag
     1021 cagtttacta aaaaattgga atgaacagac aggaaaaaat ttgacagcta atgatcttat
     1081 tggcaaatca gtctcagcca gcattgtaga aagtgctgcc gaagctagta agattgctca
     1141 atttcaaacg aagattgtac gtgtaatcaa tgatgaagat gacatggagg acagcaacag
     1201 tttcatgctg tctcatcaaa tggaaacgat tttgaaagag gctggattta cgaaagctgt
     1261 atcttatttt atcttggaac tcaaagatcc atcacagaca aaagtagtaa cagaagaatt
     1321 acagaaaaat aagaagtata ctgtgctttc tcaacagaga gttcttgata ttgtgattac
     1381 ctttattcgt gttattcagg gattattgat tgtgctttca tcacaagcta ttgtggtagc
     1441 agcggttatg attggtatca ttatttacat caatatcatg caacgttcca aggaaatagg
     1501 tgtcatgaaa gcagttggtt atcagaatcg tggtgtcaaa ggaattttta tttacgaggc
     1561 tatctggatt gtaggcatcg ccttgctgct ggcatttttg gttgcacaag gggtgggaag
     1621 tttggcgaat gcgattgtaa gtcactttta cccatccatc actaaggttt ttgaattaaa
     1681 tcttttatct gttttaggaa ctctagtttt cgctctatta cttggttatg tctcagccta
     1741 cttcccggcg cgtaagatta gtaaaatgga tcctgtagaa tcgttacgct atgaataata
     1801 agggattcct tgatttacag tcttaaaaag aaactcacat agaaaaacaa tggccatact
     1861 tgcaattcaa ctcaaatagt tgtataatag gtttgttgaa aggtgatttg tagtgattca
     1921 agttactctt ttcacaataa aaatttcatg attttcataa ggaggaaatc actaatggta
     1981 gttaaagttg gtattaacgg tttcggacgt atcggtcgtc ttgctttccg tcgtatccaa
     2041 aacgtagaag gtgttgaagt tacacgcatc aacgacctta cagatccagt tatgcttgca
     2101 cacttgttga aatacgacac aactcaaggt cgtttcgacg gtactgttga agttaaagaa
     2161 ggtggatttg aagttaacgg taaattcatc aaagtttctg ctgaacgtga tccagaacaa
     2221 atcgactggg ctactgacgg tgtagaaatc gttcttgaag ctactggttt ctttgctaag
     2281 aaagaagcag ctgaaaaaca ccttaaaggt ggagctaaaa aagttgttat cactgctcct
     2341 ggtggaaacg acgttaaaac agttgtattc aacactaacc acgacgttct tgacggtact
     2401 gaaacagtta tctcaggtgc ttcatgtact acaaactgct tggctccaat ggctaaagct
     2461 cttcaagaca actttggtgt tgttgaagga ttgatgacta ctatccacgc ttacactggt
     2521 gaccaaatga tccttgacgg accacaccgt ggtggtgacc ttcgccgtgc tcgcgctggt
     2581 gctgcaaaca tcgttcctaa ctcaactggt gctgcaaaag ctatcggtct tgtaatccca
     2641 gaattgaatg gtaaacttga tggatctgca caacgcgttc caactccaac tggatcagtt
     2701 actgaattgg tagcagttct tgaaaagaac gttactgttg atgaagtgaa cgcagctatg
     2761 aaagcagctt caaacgaatc atacggttac acagaagatc caatcgtatc ttcagatatc
     2821 gtaggtatgt cttacggttc attatttgac gcaactcaaa ctaaagttct tgacgttgac
     2881 ggtaaacaat tggttaaagt tgtatcatgg tacgacaacg aaatgtcata cactgcacaa
     2941 cttgttcgta ctcttgaata cttcgcaaag attgctaaat aattcttgag ttgatagaaa
     3001 gcaaggcttt gtggtcttgc ttttttatat ggaaaaatgg atgacacgat catccattct
     3061 tttttaattc tttttcaaat gtatttgaaa gggtagtgaa agttagcctc tctaaagtaa
     3121 gtgggtgggt aaaggaaagt cggaaggcat gaagcataag ccggcttgtc tttgatttac
     3181 tattatagag agggtctccc aggataggaa gattatgatg caaaaggtgc acacgaatct
     3241 gatgggttcg ccctgtcttt agcttgcaat gagccaagga agtcttgttt gagaattgct
     3301 ttaatctgct tacatgcgtt tcagcatatt gcccattttt tgcatcaact attctttttc
     3361 tacgatcatg gcgatcacgt ccaattttgt ctctgaaaac aagttctttt ctgttgatat
     3421 ttccatcaac tagagcccaa tattctctag aaatctcttt tttctccaat aagcgattga
     3481 gaatgggcag gataaaagga tttttggcaa agagaactaa gccactggtt tccatgtcca
     3541 gacgatgaac gacatagcag gtttggccaa cataggtact gacatggtta agaagggcaa
     3601 tttcgtttgg ttgattacca tgcgttttca tcccctctgg tttgtttaca ataatcaagt
     3661 gttgatcttg ataaacttcc tgcactaagt ctgggttgcc ccaagggatc gtcttttggg
     3721 gataatcttc ctcgtcaaaa gtcaactggc aaacatctcc aggatttacg atttcgttcc
     3781 agcggacttc ttcttgattt atcaaaatat gtttcttgat tctcaaaaaa tgacggattt
     3841 ttctagggat gaggagttgt tcctcaagta attgctttac cgtcatttga ggtagagagg
     3901 cgggtaatgt aaatgtgaat tgcatacaga tattgtaaca aaaaaagccc tatttggata
     3961 ggaaatagct aaattcttgt cttcctatga tgaagatgat aaaataaacg catgaaatta
     4021 gataaattat ttgagaaatt tctttctctt tttaaaaaag aaacaagtga actagaggac
     4081 tctgattcta ctatcttacg tcgctctcgt agtgatcgaa aaaaattagc ccaagtaggt
     4141 ccgattcgaa aattctggcg tcgttatcat ctaacaaaga ttatccttat actaggtttg
     4201 agtgcaggct tgctagttgg aatctatttg tttgctgtag ccaagtcgac caatgtcaat
     4261 gatttgcaaa atgccttgaa aactcggact cttatttttg accgtgaaga aaaagaggct
     4321 ggtgccttgt ctggtcaaaa gggaacctat gttgagctga ctgacatcag taaaaacttg
     4381 cagaatgctg ttattgcgac agaagaccgt tctttctata aaaatgacgg gattaactat
     4441 ggccgtttct tcttggctat tgtcactgct ggacgttcag gtggtggctc taccattacc
     4501 caacagctgg ctaaaaacgc ctatttatcg caggatcaaa ctgttgagag aaaagcgaaa
     4561 gaatttttcc ttgccttaga attaagcaaa aaatatagta aggagcaaat tctaaccatg
     4621 taccttaaca acgcttattt tggaaatggt gtgtggggtg tagaagatgc gagtaagaaa
     4681 tactttggag tttctgcatc agaagtgagt ctggatcaag ctgcgactct ggcagggatg
     4741 ctcaaggggc cggaactgta taatcccttg aattccgtag aagattctac taatcggcgc
     4801 gatactgtct tgcagaatat ggttgcagca ggatatattg ataaaaacca agaaaccgaa
     4861 gctgctgaag ttgatatgac ttcgcaattg cacgataagt atgaaggaaa aatctcagat
     4921 taccgttacc cctcttattt tgatgcggtg gttaatgaag ctgtttccaa gtataatcta
     4981 acagaggaag agattgtcaa taatggctac cgcatttaca cagagctgga ccaaaactac
     5041 caagcaaata tgcagattgt ttatgaaaac acatcgctat ttccgagggc agaggatgga
     5101 acgtttgctc aatcaggaag tgtagctctc gaaccgaaaa cagggggagt tcgtggagtt
     5161 gtcggtcaag ttgctgacaa tgataaaact ggattccgga atttcaacta tgcaacccaa
     5221 tcaaagcgta gtcctggttc tacaattaag cctttagttg tttatacgcc agcagttgaa
     5281 gcaggctggg ctttgaataa gcagttggat aaccatacca tgcagtatga cagctataag
     5341 gttgataact atgcagggat caaaacgagt cgagaagttc ctatgtatca agccttggca
     5401 gaatcgctta atctacctgc tgttgccact gttaatgatt tgggtgtcga caaggctttt
     5461 gaggcaggcg aaaaattcgg actcaacatg gaaaaggtcg accgtgttct tggtgtcgcc
     5521 ttgggaagcg gtgttgaaac caaccctctt caaatggctc aagcatacgc tgcctttgca
     5581 aatgaaggtt taatgcctga agctcatttt attagtagaa ttgaaaatgc tagtggacaa
     5641 gttattgcga atcataaaaa ttcacaaaaa cgggtgattg ataagtctgt agctgacaag
     5701 atgaccagta tgatgttggg gactttcacc aacggtaccg gtattagttc atcgcctgca
     5761 gactatgtca tggcagggaa aactggaaca actgaagcag ttttcaatcc ggagtacaca
     5821 agtgaccagt gggtaattgg ttatactccg gatgtagtga ttagccactg gcttggcttt
     5881 ccgaccactg atgaaaatca ctatctagct ggctctactt caaacggtgc agctcatgtc
     5941 tttagaaaca ttgccaatac cattttacct tatacgccag gaagtacctt tacggttgaa
     6001 aatgcttata agcaaaatgg aattgcacca gccaatacaa aaagacaagt acaaaccaat
     6061 gataatagcc agacagatga taatttgtct gatattcgag ggcgtgcgca aagtctagta
     6121 gatgaggcta gccgggctat ctcagatgcg aagattaagg aaaaggctca aacaatatgg
     6181 gattcgatag tcaatctatt tcgctaagat gcttgtcaaa gcctagcttt cttgttataa
     6241 tggataagat ggaggcgtta tggcactaaa aaaagcaagc ctagcttgtg cggtttgtgg
     6301 ttcgagaaac tattcaatca agatcagcgg aaaccccaag cctacacgac tagaagtaaa
     6361 taaattttgt aagcattgtg gcaagtacac tacacacaga gaaacgagat aggagagagc
     6421 gatgcgtttt attggagata tttttagact tcttaaagac acaacatggc caactcgcaa
     6481 ggaaagctgg agagattttc gttctatcat ggaatacaca gctttctttg tagtaattat
     6541 ttacattttt gaccagttga ttgtttcagg tttgattcga tttattaaca ttttttagaa
     6601 gattagtgga gttaattaca ctagaaatct tctatttatg aaaggaaata tcatggatag
     6661 ttttgataaa ggatggtttg ttttacaaac ttattctggt tatgaaaata aggtgaaaga
     6721 aaatctatta caacgtgcac aaacctacaa tatgttggat aatattctac gcgttgaaat
     6781 tccaacacaa acagtgcaag ttgaaaaaaa tggaaagaga aaagaagtag aagaaaatcg
     6841 ctttccaggt tatgttcttg tagaaatggt catgacagat gaagcttggt ttgttgttcg
     6901 aaacacacca aatgttacag gatttgtcgg atcacacggg aacagatcaa aaccaactcc
     6961 attattggaa caagaaattc gtgacatttt ggtatctatg ggacaaactg ttcaagaatt
     7021 tgatttcgat gttgagattg gtcaaactgt acgtattatt gatggtgctt ttgcagacta
     7081 cactggtaag attacagaaa ttgataataa caaagtgaaa atgattatct ctatgtttgg
     7141 taatgacaca gttgcagaag taaacctaaa ccaaatcgca gaattataac cctgagagag
     7201 gctggagcct ctcttttttg tgcagtttag gagctaaagg gaacagaatg gagaaaatgg
     7261 aacaaatgtg ttttctaatc tgttagactg tatctagaaa ggggaaaatt atgattaaag
     7321 aattgtatga agaagtccaa gggactgtgt ataagtgtag aaatgaatat taccttcatt
     7381 tatgggaatt atcggattgg gaccaagaag gcatgctctg cttacatgaa ttgattagta
     7441 gagaagaagg actggtagac gatattccac gtttaaggaa atatttcaaa accaagtttc
     7501 gaaatcgaat tttagactat atccgtaagc aggaaagtca gaagcgtaaa tacgataaag
     7561 aacccta
//
LOCUS       DAVLXF010000090         7559 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLXF010000090 DAVLXF010000000
VERSION     DAVLXF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.090"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            79..621
                     /locus_tag="VYO12_001802"
     CDS             79..621
                     /locus_tag="VYO12_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1588352.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            621..1250
                     /gene="nth"
                     /locus_tag="VYO12_001803"
     CDS             621..1250
                     /gene="nth"
                     /locus_tag="VYO12_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1588353.1"
                     /translation="MVLSKKRARKVLEEIITLFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            1461..1982
                     /gene="pyrR"
                     /locus_tag="VYO12_001804"
     CDS             1461..1982
                     /gene="pyrR"
                     /locus_tag="VYO12_001804"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1588354.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            2001..2924
                     /locus_tag="VYO12_001805"
     CDS             2001..2924
                     /locus_tag="VYO12_001805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1588355.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            2974..4053
                     /gene="carA"
                     /locus_tag="VYO12_001806"
     CDS             2974..4053
                     /gene="carA"
                     /locus_tag="VYO12_001806"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1588356.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            4366..7542
                     /gene="carB"
                     /locus_tag="VYO12_001807"
     CDS             4366..7542
                     /gene="carB"
                     /locus_tag="VYO12_001807"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1588357.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         2203 a         1545 c         1712 g         2099 t
ORIGIN      
        1 tttaattaca aagtttggtg tgctgatttg tagccttttc atggtataat caagagaata
       61 aatctttatg gaggaagtat gaaattaaat attcaagaaa ttcgtaagca gtctgaaggt
      121 ttgaactttg aacaaacgtt agatttagtt gatgacctgc gtgcacgtaa tcaagaaatt
      181 ttagatgtaa aagatatcct tgcagttggg aaagtacaat atgaagaccg tatgtatttc
      241 ttagattatc aactatctta taccattgtt cttgcttcga gtcgcagtat ggagccagtt
      301 gagttagttg aatcttatcc agtcacggaa gttttcatgg aaggcgcaac taaccagcta
      361 gatcaagaag ttttagatga tgacttggtc ttgcccatcg aaaatgggga gcttgacctt
      421 gctgagagtg tatcagacaa tatcctgcta aacattccta tcaaggtctt gacggctgaa
      481 gaagaagctg gtcaaggatt tatctcagga aatgactggc aaatcatgac agaggaagaa
      541 taccaagctc aaaaagcagt aaagaaagaa gaaaacagtc cttttgctgg cttacaagga
      601 ctatttgacg gagatgaata atggtcttgt caaaaaaacg agcacgaaag gtgctagaag
      661 aaatcattac cctcttccca gatgccaagc ctagtcttga ttttaccaat cattttgaac
      721 tcctggttgc ggtcatgtta tcagcccaga cgacagatgc agcggtaaat aaggccacac
      781 caggtctctt tgttgccttt ccaacacccc aagctatgtc tgtagcgaca gagagtgaga
      841 ttgcttcaca tatttctcgc ttgggattgt atcggaataa agctaaattc cttaaaaaat
      901 gtgcccaaca gttactagac gattttgatg gtcaagtccc tcagacacgt gaagaattgg
      961 agagtttggc aggtgttggt cgcaagacag ccaatgttgt catgagtgta ggatttggga
     1021 ttccagcctt tgcagtggat actcatgtgg agcgtatttg caaacaccac gatattgtca
     1081 aaaaatcagc gacgccactt gaggtggaaa agcgggtcat ggatatcttg ccgcctgagc
     1141 agtggttagc tgcccatcag gccatgattt attttggaag agccatttgc catccaaaaa
     1201 atccagagtg tgaccagtac ccacaattat atgattttag caatttgtaa gatgctacat
     1261 tgaaactttt tgcttgattt taagcatgct ttgtgctata gtaaatgata actaaatatt
     1321 cctttaaacc tgtcccgtga ggcaggcaag gagcgcgata aagtagaagg gtgaagtcta
     1381 tactgtgtgc agtagggctc gcttggctat aaatttcaag tctccttgtt taaggagact
     1441 tttctttttg gaggtttgtc atgaaaacaa aagaagttgt agacgaattg accgtcaaac
     1501 gagcgattac gcgtattact tatgagatta tcgaacgcaa caaagatttg aataagatcg
     1561 tcttggctgg tattaaaact cgtggtgtct ttattgccca ccgaatccaa gaacgtttga
     1621 agcagttaga aaatctttca gttcctgttg tggaattgga tactaaacct ttccgtgatg
     1681 atgttaaaag tggagaagat acttctttgg tttctgtcga tgtgacagac cgcgaagtta
     1741 tcttggtgga tgatgtgctc tatacaggtc gtaccatccg tgctgctatt gataatattg
     1801 tcggtcatgg tcgtcctgcg cgtgtgagtt tagcagttct agtcgatcgt ggacatagag
     1861 aattgccaat ccgtccagat tacgttggaa aaaatatccc aaccagtcgt tctgaagaaa
     1921 tcatcgtaga gatgacagaa cttgatgacc aagacagagt tctgattact gaagaagctt
     1981 agaaagttaa aggagtagcc atgtcagaaa atcaacaagc attgaaccat gtggtgtcca
     2041 tggaagacct cactgtcgat caagtaatga aattgatcaa gcgaggaatt gagtttaaaa
     2101 atggagccca gcttccctat gaagaccatc cgattgtttc caatctcttc tttgaggatt
     2161 ctacacggac acataagtcc tttgaagtcg cagagattaa acttggattg gaacgacttg
     2221 actttgatgt gaagactagt tcggttaata agggtgagac actttatgac accattttga
     2281 ctctgtctgc tttaggagtg gatgtctgtg tgattcgcca tccagaggtc gactactata
     2341 gagaattgat tgctagtcca acgattacga cttccatcat caatggtgga gatggttcgg
     2401 gccaacaccc tagccagagc ttgcttgatt tgatgaccat ttatgaggaa tttggccact
     2461 ttgagggtct taaagttgct attgcaggtg acttggatca ctcacgcgtt gccaaatcca
     2521 atatgcagat tttgaaacgc ttgggagctg aactcttttt cgctggacct gaggaatgga
     2581 gaagtcaaga gtttgcagac tatggacagt ttgtaaccat tgatgaaatc attgatcagg
     2641 ttgatgtcat gatgtttctc cgtgtgcaac acgaacgcca tgatagtgga gctgtatttt
     2701 caaaagaaga ctaccatgcc caacatggct tgactcaaga acgttacgat cgtttgaaag
     2761 aaacagcaat cctcatgcac ccagctccaa tcaatcgtga tgtagaaata gcagaccact
     2821 tggttgaagc accaaaatca cggattgtcc aacaaatgac caatggtgtc tttgttcgaa
     2881 tggcaatctt agaatccgta ctagcgagta gaaacgccaa ctaaaacaca agacgttaaa
     2941 agacgccagt gagcgtccac gagaggtgaa aatatgacaa aaagaattct agtattagaa
     3001 gatggcacag tttttgaagg caaggccttc ggagcagata ttgatgtaac aggcgaaatc
     3061 gtctttaata cagggatgac cggctaccaa gaatccatta cagaccagtc ttataatgga
     3121 caaatcttga cctttactta tcctttggta ggaaattatg gtattaatcg tgatgattat
     3181 gaatccatta ttccaacttg taagggagtc gttgtttttg aagaagcgcg tagagctagc
     3241 aactggcgca accaaatgac cttggatgaa tttttgaaag ccaaaaaaat tccagggatt
     3301 tcaggaattg atacgcgtgc tcttaccaag attatccgta agcatggtac tatgcgtgca
     3361 accttgaccc atgttgggga cagtatggac catgtgacgg accagctcca agcaacagtt
     3421 ttaccgacag acaatatcaa acaggtttct actaaaacgt cttatccagc tccaggagtt
     3481 ggtttgagcg tggtgctagt ggactttggt ctcaagcact caatcctacg tgaactttct
     3541 aaacgcaact gtaacgtgac agttgttcct tattcgacaa cggctgaaga aattctccat
     3601 ctcaatcctg atggggttat gttgtcaaat ggtccaggta acccagaaga cgttccacaa
     3661 gcactcgaca tgattcgcgg tgtacaagga aaaattccaa tctttggtat ttgtatggga
     3721 caccaactct ttgcaatggc aaacggggct aagacctata agatgaagtt tggccaccgt
     3781 ggattcaacc atgcggtacg tgaaatcgca acaggacgag tagacttcac cagccagaac
     3841 catggttatg cagtcagccg cgaggatttg ccagagcact tgattattac ccacgaagaa
     3901 atcaatgaca agtcagtgga aggtgtgcgc cacagatacc aacctgcttt ctctgttcaa
     3961 taccacccag atgcagctcc tgggccacac gacgctagct acctatttga cgagtttatc
     4021 gagatgatgg aagtttttaa acaatcaaac taagaacgag taaaagctcg tcggagaatt
     4081 tatgtaactg aacacggacg gaaagctcag aatgtagaga aatcaacttg gattgtcaat
     4141 ccttccttga ttttctaaaa ttcaatcgct ttctattcgt cctttgtatc ttatttaatg
     4201 tcgccctgag aggcgacgaa tagaaaggag aagggtgctc cgtatttgct gaactgaata
     4261 cgggctacgg acggtgctaa aaagatagtt attcctagaa ctgacagttc ttcgtcataa
     4321 ctcctatttt agctttgtcc gcttgacgcc cttaatatct tatatatgcc taaacgtact
     4381 gatattcaaa aaattatggt gattggttct ggtccgatta ttattggcca ggctgctgag
     4441 tttgactacg ctgggactca ggcttgcttg tcgttgaaag aggaaggtta tgaggttgtt
     4501 ttggttaact caaatcctgc aaccatcatg acggacaagg aaattgctga caaggtttac
     4561 attgaaccga ttacacttga gtttgtgaca cgtattcttc gtaaggaacg tccagatgcc
     4621 ttgctaccaa cactcggtgg tcagacaggt ctcaacatgg ccatggaatt atctaaaaat
     4681 ggtatcctag acgagcttgg cgttgaactt ctgggtacca aattatctgc cattgaccaa
     4741 gcggaggacc gtgacctctt taaacaattg atggaagagc ttgaacaacc cattccagaa
     4801 tctgaaattg ttaacacagt agaagaagca gttgcctttg cagcgacaat tggctaccca
     4861 gtcatcgttc gtccagcctt tacccttggt ggtactggtg gtggtatgtg tgccaacgag
     4921 aaagaattgc gtgaaatcac tgaaaatggt ttgaaattgt cacctgttac ccaatgtttg
     4981 attgagcgtt cgattgccgg tttcaaggaa attgaatacg aagtgatgcg tgactcagct
     5041 gacaatgcct tggttgtttg taacatggaa aactttgacc cagttgggat tcacacaggg
     5101 gattccattg tatttgcccc tgcgcaaacc atgtcagact atgaaaacca aatgcttcgt
     5161 gatgcgagct tgagcattat tcgtgccctc aagattgaag gtggatgtaa tgttcagcta
     5221 gctcttgatc ctaacagctt caagtattat gttatcgaag taaaccctcg tgtatcacgt
     5281 tcgtcagccc ttgcttctaa agcgacagga tatccgattg ctaagttggc tgccaagatt
     5341 gcagtaggtt tgaccttgga tgaggttata aacccagtta caggttcaac ctatgccatg
     5401 tttgaaccag cccttgacta cgtagttgct aagattccac gtttcccatt tgacaagttt
     5461 gaaaagggtg aacgccgtct cggtacacaa atgaaggcga ctggagaagt catggcaatc
     5521 ggtcgtaaca tcgaggaatc tcttctcaag gcctgccgct cccttgaaat tggggtgcac
     5581 cacaatgaaa tacctgaact tgcagcagtt tctgatgatg ccttgattga aaaggttgtc
     5641 aaagcccaag atgaccgtct cttctacgta tcagaagcaa ttcgtcgtgg ctacacacct
     5701 gaagaaattg cagagcttac aaaaattgat atcttctatc ttgataaact cttgcatatc
     5761 tttgaaattg agcaagaatt gggtgctcat ccacaagatc tagaagtttt gaaaacagcc
     5821 aaacttaatg gtttttcaga ccgtaagatt gctgaactct ggggaacgac tgatgataaa
     5881 gttcgccaac ttcgcttgga aaacaagatt gttccagttt ataagatggt agacacttgt
     5941 gcggcagagt tcgactccga aacgccatat ttctattcaa cctatggttg ggaaaatgaa
     6001 tctatcagat ctgataaaga atctgtactt gtcctaggtt caggtccaat ccgtatcggt
     6061 caaggggttg agtttgacta cgcaaccgtt cactctgtta aggctatcca ggcagctggt
     6121 tatgaagcta tcatcatgaa ctcaaaccca gagaccgttt caacagactt ctctgtatca
     6181 gataagcttt actttgagcc attgacattc gaagatgtta tgaacgtcat tgacttggaa
     6241 caaccaaagg gagttatcgt tcagttcggt ggtcaaacag ccatcaacct tgcggagcca
     6301 ttggcaaaag caggtgtgac catccttggt acacaggttg ctgacctaga ccgtgccgaa
     6361 gaccgtgacc tcttcgagca agctctcaaa gaattggata ttccacagcc accaggacaa
     6421 acggctacca atgaagaaga agcagctctt gcagctcgta agattggctt cccagttctt
     6481 gtccgcccat cttatgtact tggtggacgt gccatggaaa tcgttgaaaa cgaagaagac
     6541 cttcgttctt acatgcgaac tgctgttaag gctagtccag atcacccagt tcttgtcgac
     6601 tcttatatcg ttgggcaaga gtgcgaagtt gatgccattt cagatggtaa agatgtactc
     6661 attcctggta tcatggagca tatcgaacgt gctggtgtcc actcaggtga ctcaatggct
     6721 gtttacccac cacaaacctt gtcgcaaaag gttcaggaga ccatcgcaga ctacaccaaa
     6781 cgcttagcaa tcggtcttca ctgccttgga atgatgaaca tccagtttgt tatcaaggat
     6841 gaaaaagtct acgttattga ggtcaatcca cgtgccagcc gtactgttcc attcctatct
     6901 aaggtaacca atattcctat ggctcaagta gcgaccaagc tcattcttgg tcaaagttta
     6961 tcagaacttg gctaccaaaa tggactttac cctgaaagca cccgtgttca tatcaaggca
     7021 cctgtcttct cctttaccaa actagctaag gtagacagct tgctcggtcc tgaaatgaag
     7081 tcaacaggtg aagttatggg ttctgatgct actttggaaa aagctctcta taaagccttt
     7141 gaagcttctt atctacactt gccaactttt ggtaatgtcg tctttaccat cgctgatgat
     7201 gccaaagaag aagccttgaa cttggctcgt cgtttccaaa acattggtta tggaatcctt
     7261 gcgacagaag ggacagcagc cttctttgct agccatggct tgcaagccca acctgttggt
     7321 aagattggtg atgacgataa ggatatccca agttttgtac gcaaaggaag aattcaagct
     7381 atcattaaca cagtaggaac aaaacgaact gctgacgaag atggtgagca aattcgtcgt
     7441 tcagccattg aacacggggt gcccctcttc acagcgctag atacagctaa tgccatgctt
     7501 aaggtacttg aaagtcgtag ctttgttaca gaagcgattt aaatagaaaa ataagatat
//
LOCUS       DAVLXF010000091         7405 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLXF010000091 DAVLXF010000000
VERSION     DAVLXF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.091"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..1988
                     /locus_tag="VYO12_001808"
     CDS             <1..1988
                     /locus_tag="VYO12_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1588358.1"
                     /translation="NKEALLLGMSYLERWYNFSYGQVNVKDLVMYHPDFFGKGNTSPL
                     DTLIELGKSGFNNLLAKNNVDTYAISLASHHGTTDLFSTLENYRKVFLPDKTNNDWFK
                     SQTKAYIVEEKSNIEEVKTKQGLVGTKYSIGVYDRITSATWKYRNMVLPLLTLPERSV
                     FVISTISSLGFGAYDRYRNKEHQANGDLNSFVEKSAHETAERQRDHYDYWYRILDEKG
                     REKLYRNILLYDAYKFGTNHTEGKATEVADFDSPNPAMKHFFGPVGNKVGHNGHGAYA
                     TGDAVYYMGYRMLDKDGAITYTHEMTHNSDQDIYLGGYGRRSGLGPEFFAKGLLQAPD
                     QPSDATITINSILKHSKSDSKEGERLQVLDPTTRFKDATDLQKYVHNMFDVVYMLEYL
                     EGKSIVKKLNVYQKIEALRKIENQYLTDPADGNDVYATNVVKNLTEDEAKKLTSFDSL
                     IDNNILSAREYKAGTYERNGYFTIKLFAPIFSALSGEKGTPGDLMGRRIAFELLAAKG
                     FKDGMVPYISNQYEEDAKQQGQTINLYGKERGLVTDELVLKKVFDGKYKTWAEFKTAM
                     YQERVDQFGNLKQVTFKDPTKPWPRYGTKTINNVDELQKLMDEAVLQDAKERNYYYWN
                     NYNPETDSAVHKLKRAIFKAYLDQTNDFRRSIFENKK"
     gene            2032..>7405
                     /locus_tag="VYO12_001809"
     CDS             2032..>7405
                     /locus_tag="VYO12_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1588359.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGTSTYHYLDYS
                     TLTQTERVQLKQGRPDESKESYALVYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DKKRKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVDYLELDDR
                     TVSPVEQPKVVTDKGEPEVQPALPEAVVTGKGKTEVQPTLPEAVVTEKGEPEVQPVLP
                     EAVVAEKGEPEVQPVLPEAVVAEKGEPEVQPALPEAVVTEKGEPEVHEKPDYTQPIGA
                     NLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEPISTVSEVASEREELPS
                     LHTDIRTETIPKTTIEESDPSKFIGDNSVKQVGEDGERQIVTSYEELHGKKISESVET
                     VTILKEMKPEIIVKGTKERPKEKTAPVLTLTKVTEDAMNRSANLNYELDNKDNAEISS
                     IVAEIKDGDTVVKRVDLSKEKLTDAVQNLDLFKDYKIATTMIYDRGQGSETSKLDERP
                     LRLELKKVEIKNIASTNLVKVNDDGTETPSDFMNEKPSEEDVKKMYLKITSRDNKVTR
                     LTVDSIEEVTEEGQKLYKITAEAQDLIQHTDPTKVRNKYVYYIEKPHPKEDNVYYNFK
                     DLVDAMNTDKNGTFKLGADLNATGVPTPKKWYVDGDFRGTLKSVEGKHYTIHNTERPL
                     FQNIIGGTVTKVNLGNVNINMPWADRIAPIADTIKGGAKIEDVKVTGNVLGRNWVSGF
                     IDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVERAYVDANLIGKKAKAA
                     GIAYWTQNSGDNHKVGVEGAVKKGIVKGTIQVESPVEVGGAVGRLSHHGYIGEVVSMM
                     KVKKGEIFYGSSDMNDDPYWVANNVRGNYVVNGVSEGTISYARAKEHHRIKPISQSEA
                     DTKIMMLGITAQDFAINEPVVNRLNRLTRKEDEYKSTQDYKVDRDLAYRNIEKLQPFY
                     NKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGTKEEMDV
                     TKNADSKVKQVREYTIAGQNVVYTPNMVEKDRNQLIQDIKDKLASVQLISPEVRALMD
                     ARKKPEENTDERKNGYIKDLYLEESFAETKANLDKLVKSLVENADHQLNSDEAAMKAL
                     VKKVDENKAKIMMALTYLNRYYDIKYGDMTIKNLMMFKPDFYGKSVDLLDFLIRIGSS
                     ERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFTNDTDLNDWFIHAAKNVYVSE
                     PQTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAHMFLISTYNTMAYSSFEKYGK
                     YTEEARNEFKKEIDKVAHAQQTYLDFWSRLALPSVRDQLLKSENRVPTPVWDNQNYSG
                     IKGINRMGYDEKKVPIAPIRELYGPTWKFHNTNWNMGAMASIFPNPNNNDQVYFMGTN
                     MISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAYAQGMLQTPSSIGHQGEYGAL
                     GLNMAYHRENDGDQWYNYDPDKLQTREDIDRYMKNYNEALMMLDHVEADAVLPQLNGD
                     NSKWFKKIDREMRRNLGDGLNNLVAPHQWDNVRDLNQEESSKKLSSINDLIDNNFMTK
                     HGNPGNGRYRPEDFRPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYD
                     KGFTSYVSSKYQGEADKQNQGRLGDDFIIKKVSNNQFSNLEDWKKYWYHDVKSRAEKG
                     FTEITIDGQTIHNYNELKDLFDKAVTEDLKKAGNYSNTENLKSKVFKALLKNTDGFFN
                     PLFKKDI"
BASE COUNT         2604 a         1161 c         1560 g         2080 t
ORIGIN      
        1 ggaataagga agccttactt cttggaatga gttatttaga acgttggtat aactttagct
       61 atggtcaagt gaatgtcaaa gacctagtta tgtatcatcc ggacttcttt ggtaaaggaa
      121 atacttcacc attagatact ctgattgagt taggtaaatc tggctttaac aatcttcttg
      181 ctaaaaacaa tgtcgatact tatgctatca gccttgccag ccatcatgga acgacagatt
      241 tgtttagcac gttggaaaat taccgaaaag tctttctacc agataaaacc aataatgact
      301 ggtttaaatc acagactaag gcttacattg tcgaagaaaa atccaatatc gaagaggtga
      361 aaacgaagca aggactagtt ggcactaagt attctattgg tgtctatgac cgtatcacta
      421 gtgccacatg gaaataccgt aatatggtac tacctctact taccttgcca gaaagatccg
      481 tatttgtcat ttcaaccata tctagtctag gttttggtgc ttatgatcgc tatcgtaata
      541 aagaacatca agcaaatggt gatcttaata gctttgttga aaagagtgct cacgaaacag
      601 cagagcgtca acgagatcac tatgattatt ggtatcgtat tctagatgaa aaagggcgtg
      661 aaaagcttta tcgaaatatc ttgctttatg atgcctataa atttggaact aatcatactg
      721 aaggaaaggc tacagaagta gcagattttg atagtccaaa tcctgcaatg aaacatttct
      781 ttggacctgt tggaaataag gttggacata atggacatgg tgcttatgct acaggggatg
      841 cagtatatta tatggggtat cgcatgctag ataaggatgg agctattacg tatactcatg
      901 agatgacgca taattcagat caagatattt atcttggtgg ttatggaaga agaagtggac
      961 ttggacctga gttctttgct aaaggcttac tacaagcacc agaccaacca agtgatgcaa
     1021 cgattactat caattctata ttgaaacatt caaaatctga tagtaaagaa ggagaacgat
     1081 tacaagtact tgatccaact acgagattta aagacgcaac agatcttcaa aaatacgtcc
     1141 acaatatgtt tgatgttgtt tatatgttgg aatatctcga aggtaaatct attgttaaga
     1201 aattaaatgt atatcagaaa atagaagctc tcagaaaaat cgagaaccaa tatctaacag
     1261 atcctgctga tggaaatgat gtttatgcta ctaatgttgt gaaaaatttg actgaagatg
     1321 aagctaaaaa attgacgagc tttgatagtt tgattgacaa taacattctt tcggctcgtg
     1381 agtataaggc tggcacttat gagagaaatg gctactttac tataaaactc tttgcaccaa
     1441 tcttttcagc attgagcggt gagaaaggaa ctccaggtga ccttatggga cgaaggatag
     1501 cttttgaact tctagctgct aaaggattta aagatggtat ggtaccatat atttccaacc
     1561 aatacgaaga agatgctaaa caacaagggc aaactatcaa tctttatggt aaagaacggg
     1621 gcttggtgac cgatgagctt gttttgaaaa aggtatttga tggtaagtat aaaacttggg
     1681 cagaatttaa gacagctatg taccaagaac gggtggatca gtttggaaac ttgaagcagg
     1741 tgacctttaa agatccgaca aaaccatggc caagatatgg cacaaagact atcaataatg
     1801 tggatgaatt gcagaaacta atggatgaag ctgttttgca ggatgcaaaa gaacgtaatt
     1861 attattattg gaataactat aatccagaaa cggacagtgc cgtccataag ttgaagagag
     1921 caatctttaa agcctatctt gaccaaacaa atgattttag aagatcaatt tttgagaata
     1981 aaaaatagtg tttactatta ggaaataaaa agtgaatgtg gaggattaga attgtcatta
     2041 ttaaaaaaag ataaattttc cattcggaaa ataaagggta ttgttggttc ggtattcttg
     2101 gggagtcttt tatttgcacc gtcagttgtt ggtacatcga cttatcatta cttagattat
     2161 agtactttga cacaaactga acgtgttcaa ctcaaacaag gtagacctga tgaatcaaaa
     2221 gagtcatatg ctttggttta tgaaaaagac gcattaccaa acacaggaag ttctcaatct
     2281 attatgactg cattgggttt gttagctatc gggagtctta ttgtgattat cacaaaagat
     2341 aaaaagagga aaaaaatcgc tacatttttg attgtagggg cgacaggttt agttacacta
     2401 tcaactgctt cagcacttaa tttgaatgct aacatccatg agtctggacg tgatggtgtg
     2461 ttgcaaattt ctggttacag atatgttgat tacttggagc ttgatgatag aactgtttct
     2521 ccagttgagc aacccaaagt tgtgaccgac aaaggcgaac cagaagttca gccagcgtta
     2581 ccagaagctg ttgtaactgg aaaagggaaa actgaagtac aaccgacatt accagaagct
     2641 gttgtgactg aaaaagggga accggaagta caaccagtgt taccagaagc tgttgtggct
     2701 gaaaaagggg aaccggaagt acaaccagtg ttaccagaag ctgttgtggc tgaaaaaggg
     2761 gaaccagaag tacaaccagc cttacccgaa gcagttgtaa ctgaaaaagg cgaaccagaa
     2821 gttcatgaga agcctgacta tacgcaacca ataggtgcaa accttgttga gcctgaagtt
     2881 catgagaaac tagcatacac tgaacctgta ggtacaacag gagtagatga aaatggaaac
     2941 ttgattgagc cacctgttaa cgatattcca gagtatactg aaccaatatc tacagtttca
     3001 gaagttgcat cagaaagaga agagttgcct tctttacata ccgatattcg tacagagact
     3061 attcctaaaa ccactataga agaatctgat cctagcaaat ttataggcga taattctgtt
     3121 aaacaagttg gtgaggatgg cgaacgtcaa attgttacta gctatgaaga gttacatggc
     3181 aaaaaaatca gcgagtcagt tgaaacagta acaattttga aagaaatgaa gcctgaaatt
     3241 attgtaaaag gtacaaaaga aagaccaaaa gaaaaaacgg ctcctgtttt aaccttgacc
     3301 aaagtaacag aagatgctat gaatcgttct gccaacttaa attatgagct tgataataaa
     3361 gataatgctg aaatatcaag tattgtagct gaaattaaag atggtgatac agtagttaaa
     3421 agggttgatt tatctaaaga aaaattaact gacgctgtac aaaatttgga tttgtttaaa
     3481 gattataaga ttgcaacaac aatgatttat gatcgtggtc aaggttctga aacgtctaaa
     3541 ctggatgaaa gacctctgcg tttggagttg aaaaaggtcg aaattaaaaa tatcgcatct
     3601 actaatttag tgaaggtaaa tgatgatggt actgaaacac ctagtgattt tatgaatgaa
     3661 aaaccttcag aagaagatgt taaaaaaatg tacttaaaaa tcactagtcg cgataataag
     3721 gtaactcgtt taactgtaga ctctattgaa gaagtgactg aagaaggtca aaaactttac
     3781 aaaataactg ctgaagcaca agatttgatt caacacacag acccaacgaa ggttcgtaat
     3841 aagtatgttt attatataga aaaaccccat cctaaagaag ataatgtcta ctataacttt
     3901 aaagatttag tagatgctat gaatactgat aaaaatggta catttaaact tggtgcagat
     3961 ttgaatgcca ctggtgttcc aacacctaag aaatggtatg ttgacggcga ctttagaggt
     4021 acattgaaga gtgtagaggg gaaacattat actatccaca acacggagcg tccgttattc
     4081 cagaatatta ttggtggtac agtaaccaaa gtaaatctgg gaaatgtaaa tatcaatatg
     4141 ccatgggcag accgcatcgc accgattgct gacactatta aaggtggcgc taaaattgaa
     4201 gatgtcaaag tgactggtaa cgtattaggt agaaactggg tatctggatt tattgataag
     4261 attgacaacc aaggtacact cagaaatgta gcctttatcg gaaatgtgac atctgtaggt
     4321 gatggtggtc aattcttgac cggtatcgtt ggtgagaact ggaaaggact agtcgaacgt
     4381 gcgtatgttg acgcaaatct tattggcaag aaagccaagg ctgctggtat tgcttattgg
     4441 acacaaaaca gtggtgacaa tcataaagtt ggtgtcgaag gtgcagtcaa gaaaggaatt
     4501 gtgaagggta ccattcaggt agaaagtcct gttgaagtcg gcggtgctgt tggacgtttg
     4561 agtcatcatg gttatatcgg ggaagtcgta tctatgatga aagttaagaa gggtgaaatt
     4621 ttttatggtt ctagtgatat gaatgatgac ccatattggg tggcgaataa tgtacgagga
     4681 aattatgtag ttaatggtgt gagtgaaggt acgatatctt atgctcgtgc taaagaacac
     4741 catcgtatca aaccaatttc acagtcagaa gcagatacta aaattatgat gttaggtatt
     4801 acagcacaag attttgctat caacgaacct gtggtcaatc gcttaaaccg tttaaccaga
     4861 aaagaagatg aatataagtc aacccaagac tataaagtag atcgagatct ggcttatcgt
     4921 aatattgaga aacttcaacc attttataac aaagaatgga ttgtcaacca aggtaacaaa
     4981 cttgcagaag attcaaactt agctaagaaa gaagtccttt ctgttacggg tatgaaggat
     5041 ggacagtttg taactgactt atcagatatt gataagatta tggttcacta tgctgatggt
     5101 actaaagaag aaatggacgt cactaagaat gctgattcta aagtgaaaca agttcgtgaa
     5161 tataccatcg ctggacaaaa cgttgtttat acaccaaata tggttgagaa agaccgtaac
     5221 caattgattc aagatatcaa agataagttg gcgtcagttc aattgatttc tcctgaagta
     5281 cgtgctttga tggatgcacg taagaaacca gaagaaaata ctgatgaacg taagaatggt
     5341 tatattaaag acctttactt agaagaaagc tttgctgaaa cgaaagctaa tcttgataaa
     5401 ttggttaaat ccttagttga aaatgcagat catcaattaa atagtgacga agcggctatg
     5461 aaagcacttg ttaagaaggt ggatgaaaat aaagctaaaa tcatgatggc tctgacttat
     5521 ctcaatcgtt actatgatat caagtatggg gatatgacta tcaagaactt gatgatgttt
     5581 aaacctgatt tctatggtaa atcagttgac cttcttgatt tcttgattcg aattggatca
     5641 agtgagagaa atatcaaagg tgacagaact ttggatgctt atcgtgatat gattggtgga
     5701 actattggta agtctgaatt acatggattc ttagattaca atatgcgtct cttcactaac
     5761 gatactgatt taaatgattg gttcatccat gcagctaaga atgtatatgt atcagaacct
     5821 caaacaacga acccagattt tgttaataaa cgacatcgtg catttgatgg tttgaacaac
     5881 ggtgttcata atcgtatgat tctacctcta ttaactctta agaatgcaca tatgttcttg
     5941 atttcaactt acaatacaat ggcttatagt tcttttgaga aatatggtaa gtatacagaa
     6001 gaagctagaa atgaattcaa aaaagaaatt gataaggttg cacatgctca acaaacatac
     6061 ttagacttct ggtcacgttt agcattgcca agcgtacgtg accaattact caagagtgag
     6121 aatagggtac caactcctgt gtgggataac caaaactata gtggtattaa aggaataaac
     6181 cgtatgggtt atgatgagaa gaaagtacca attgcgccaa ttcgtgagtt atatggtcca
     6241 acttggaaat tccataatac caactggaat atgggagcta tggcatctat tttcccaaat
     6301 cctaataaca atgaccaagt ttatttcatg ggaacaaata tgattagtcc gtttggtatt
     6361 tcagccttta ctcatgaaac tacacacgtt aatgatagaa tgctttactt cggtggtcat
     6421 agacaccgtc aaggtacaga cgtcgaagcc tatgctcaag gtatgctaca aactccgtca
     6481 agtattggtc accaaggtga atatggtgct ttaggtttga acatggctta tcatcgtgaa
     6541 aatgatggtg accaatggta taactatgac cctgataagc ttcaaactcg tgaagatatc
     6601 gatcgatata tgaaaaacta taatgaagca ctgatgatgc tagatcatgt agaagcagac
     6661 gccgtacttc cacaattgaa tggtgataat agcaagtggt tcaagaaaat tgatagagaa
     6721 atgcgcagaa atcttgggga tggtttgaat aacttagtag caccgcacca atgggacaac
     6781 gttcgtgact taaatcaaga agagagttct aagaaattat caagtattaa cgatttgatt
     6841 gataataatt tcatgaccaa acacggaaat ccaggtaacg gacgttatag accagaggat
     6901 tttagaccaa actcagctta cgtaaatgtc aatatgatgg caggaattta cggtgggaat
     6961 acgagtcaag gtgctcctgg ttcattatcg ttcaaacata atgccttccg tatgtggggt
     7021 tattatggat atgacaaagg tttcactagt tatgtatcta gcaaatatca aggtgaagct
     7081 gataaacaaa accagggtag attaggtgat gatttcatta tcaaaaaagt ttcaaacaac
     7141 caattctcaa atcttgaaga ttggaagaaa tattggtatc atgatgtgaa atccagagct
     7201 gaaaaaggat tcacagagat tacaattgat ggacaaacca ttcacaacta taatgaactg
     7261 aaagacttat tcgacaaagc tgtcacagaa gacctcaaga aagctggaaa ctactcaaat
     7321 actgagaatt tgaaatccaa agtcttcaaa gctcttctta aaaacacaga cggtttcttt
     7381 aatccattgt tcaaaaaaga cattt
//
LOCUS       DAVLXF010000092         7285 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLXF010000092 DAVLXF010000000
VERSION     DAVLXF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.092"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..589
                     /locus_tag="VYO12_001810"
                     /pseudo
     CDS             1..589
                     /locus_tag="VYO12_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            756..956
                     /locus_tag="VYO12_001811"
     CDS             756..956
                     /locus_tag="VYO12_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588360.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            998..1402
                     /locus_tag="VYO12_001812"
     CDS             998..1402
                     /locus_tag="VYO12_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588361.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1633..2340
                     /gene="ccdA2"
                     /locus_tag="VYO12_001813"
     CDS             1633..2340
                     /gene="ccdA2"
                     /locus_tag="VYO12_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1588362.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDFNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2351..2908
                     /gene="sdbB"
                     /locus_tag="VYO12_001814"
     CDS             2351..2908
                     /gene="sdbB"
                     /locus_tag="VYO12_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1588363.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3282..4673
                     /locus_tag="VYO12_001815"
     CDS             3282..4673
                     /locus_tag="VYO12_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1588364.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4816..5733
                     /gene="adcAII"
                     /locus_tag="VYO12_001816"
     CDS             4816..5733
                     /gene="adcAII"
                     /locus_tag="VYO12_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1588365.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLAEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLEADPQNDKTYLENLEENMSVLAEELK"
     gene            5741..>7285
                     /gene="phtB"
                     /locus_tag="VYO12_001817"
     CDS             5741..>7285
                     /gene="phtB"
                     /locus_tag="VYO12_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1588366.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVLKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAQAYWNGKQGSRPSSSSSYNANTAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDISSDKVKLVEDILAFLAPIRHP
                     ERLGKPN"
BASE COUNT         2266 a         1340 c         1527 g         2152 t
ORIGIN      
        1 aggtagtata acagaagcat cacacgtttt ccaaatctca cgtaatacca tttatggctg
       61 gttaaagcta aaagagaaaa caggagagtt aaaccaccaa gtaaaaggaa caaaaccaag
      121 aaaagttgat agagatagac ttaaaaacta tcttactgac aatccagacg cttatttgac
      181 tgaaatagct tctgaatttg gctgtcatcc aactaccatc cactatgcgt tcaaagctat
      241 gggctacact cgaaaaaaag aaccacacct actatgaaca agacccagaa aagtagcctt
      301 atttcttaag aattttaata gtttaaagca cctagcacct gtttagattg atgaaacagg
      361 attcgatact tatttttatc gagaatatgg tcgctcatta aaaggtcagt taagaagagg
      421 caaagtatct ggaagaagat atcagaggat ttctttggtt gcaggtctaa caaatggtga
      481 attaatcgct ccaatgactt acgaagagat gatgacgagc gacttttttg aagtatggtt
      541 tcagaagttt ttcttaccaa cattaaccac accatcggtt attatagtaa aatgaaataa
      601 gaataggaca aatcgatcag gacagtaaaa tcgaattcta acaatgtttt agaagtccat
      661 atgtactatt ctagtttcaa tataccataa ctgttcctag tcaacctgca agccctgtcc
      721 cagctccaag ctcagctccg actcaagatc ctagagtggt agcaagcgct tcggctagct
      781 caacgagcac acaagctcaa gagcaagttg acaagtctga acttcgtgcc ttgagtcaag
      841 agttagacca acgcttgaaa gctttggcaa cagtatctga tccgaaaatc gatgcaacca
      901 aggctgtcct cctagatgct caaaaagctc cggaagatag tgccttgaca gagtaaggtc
      961 tgcatactgc agtagagtct gtgaaggctg ggctgccttg gactctctca aagatgtgaa
     1021 agcaaatgct agcgacagca agcctgcaca ggacaagaag gatgcaaaac aaggaacgga
     1081 agatagtaag gattcagata agatgactga aacaaactca gttccggcag gagtgattgt
     1141 ggtcagtcta cttgccctcc taggcgtgat tgccttctgg ctgattcgcc gtaagaaaga
     1201 gtcagaaatc cagcaattaa gcacggaatt gatcaaggtt ctaggacagc tagatgcaga
     1261 aaaagcggat aaaaaagtcc ttgccaaagc ccaaaacctt ctccaagaaa cccttgattt
     1321 cgtgaaagaa gaaaatggct cagcagagac agaaactaaa ctagtagagg agcttaaagc
     1381 aatccttgac aaactcaagt aaaacatgat ttatgaaatg tcagacctaa aagacatttt
     1441 cgttcacaaa agcagcgatg agtaatcctt gtcgttgctt tttggctatt ttgggggata
     1501 agatccgcct tgactagcaa aagaaaagat tggtccaata gtgcccgata cgattttgcg
     1561 tagcagaaag cttacaaata aaattgaata tagtaaaata ggattagaat tattaagaaa
     1621 gttggttctt tattggaaac gatagtattt ttaatctctg tttttctagc aggtgtttta
     1681 tccttttttt ctccttgtat ttttcctctt ctgccagtct atgctgggat tttattggat
     1741 gatcaagaaa gtgcaaaaag cttttctttg tttgggagaa aggttctctg gtcaggcttg
     1801 attcgaacac tttgctttat cgctggtatc tctctcattt tctttattct aggctttggt
     1861 gctggttact ttggtcatat tctctatgca aattggtttc gatatgtcat gggagctatt
     1921 attatcattt tgggtcttca ccagatggaa atttttcatt tgaagaaatt agaagttcaa
     1981 aaaagtttta cctttaaaaa atcagatttt aatcgttatt ggtcagcttt tttacttggt
     2041 attaccttta gctttggttg gacaccttgt attggtccag ttttaagttc tgttttagca
     2101 cttgcggctt ctggaggcaa tggtgcttgg caaggagcga tttatactct catttacact
     2161 ctgggcatgg cccttccttt cttggtcttg gcactagctt caggtctagt catgccatat
     2221 tttagtaaaa tcaagcgtca tatgatgcta ctaaagaaaa ttggtggttt cctcattgtt
     2281 ttaatgggaa ttttgttact attaggacaa gtaaatgttc tagctggaat ttttgaataa
     2341 aggaggacaa atgaaaaaag taatgtttgc tggcttaagt ctcttgtcat tagttgtatt
     2401 gatggcctgt ggtgaggaag aaactaaaaa gactcaagca gcacaacagc caaaacaaca
     2461 aacgactgta caacaaattt ctgttggaaa agatgctcca gactttacat tgcaatccat
     2521 ggatggcaaa gaagttaagt tatctgattt taagggtaaa aaggtttact tgaagttttg
     2581 ggcttcatgg tgtggtccat gcaagaaaag tatgccagag ttgatggaac tagcggcgaa
     2641 accagatcgt gatttcgaaa ttcttactgt cattgcacca ggaattcaag gtgaaaaaac
     2701 tgttgagcaa ttcccacaat ggttccagga acaaggatat aaggatatcc cagttcttta
     2761 tgataccaaa gcaaccacct tccaagctta tcaaattcga agcattccta cagaatattt
     2821 aattgatagc caaggaaaga ttggaaagat tcaatttggt gctatcagta atgcggatgc
     2881 agaagcagca tttaaagaaa tgaactagaa tcgataaaat ctgattacaa gatgtagtca
     2941 gattttggta acttctaaaa ctaacttcca gtttcccaca acctagcttt tagtatgaga
     3001 aaaacgcgtg acatcagtgg aaatccgtct tagactaact tccactggtt ttctttttct
     3061 tgatatataa gggttcaaaa gcgaaaagac tcagaaaaag tgcacgacaa atagccctaa
     3121 aacagagtcg tcttagagta acttccagtt ttgggagaga gatggaagtt actttgagaa
     3181 gttacggtag tcagattttt ttagaaaaac attttataaa tattcacata tctcctatat
     3241 ttatggtaaa atagaattat cagtttattt tggagtcaaa gatgaatata tttagaacaa
     3301 agaatgttag tttagataaa acagagatgc ataggcattt gaagttatgg gatttgattt
     3361 tgctgggtat cggagccatg gtagggacag gcgtctttac aatcacaggt actgcagctg
     3421 caacacttgc tggcccagcc ctagtgattt caatcgttat ttctgccttg tgtgtgggat
     3481 tatcagccct cttttttgca gaatttgcct cgcgagtacc cgctacagga ggtgcctata
     3541 gttacctcta tgctatctta ggagaattcc ctgcctggtt ggctggttgg ttaaccatga
     3601 tggagttcat gacagccata tcaggcgtag cttcgggttg ggcagcttat tttaaaggct
     3661 tgctctctca atacgggata gcctttcctc aggctttaaa tggtaccttt aatcctcaag
     3721 cagggacatt tgttgattta ttgcctattc ttgtcttggt cttggtgact tcgcttgttt
     3781 tactaaatgc caaggcagcc ttacgcttta attcgattct agttattttg aaattttccg
     3841 ctttagctct ctttgtttta gtaggaattt ggaatattaa gtttgataat tggagcaatt
     3901 ttgctcctta cggttttgga caaatctatg gagctagtac tggtattatg gctggtgcat
     3961 ccttgatgtt cttcggtttt ttgggatttg aatccatctc tatggctgta gatgaagtca
     4021 agactcctca aaaaaatatt cctagaggga ttgtcttatc gctttctatc gtaaccattc
     4081 tctatgcctt ggtgaccctt gttttgactg gtgtggttca ctatagtcat ttaaatgtcg
     4141 atgatgccgt tgcctttgcc cttcgtagtg ttgggattag ttgggcagcc aactatgtgt
     4201 cattagtggc tatcttgacc ttgattacag tttgtatttc gatgacctat gccctatcgc
     4261 gtatgattta cagtttagca cgtgacggct tagtgcctgc tgcctttaag gaactgacga
     4321 agactagcaa ggtaccaaag aatgctacta tcttgacagg tctagcttca gcagtagcag
     4381 caggaatgtt cccactagcc agtattgcag cctttttaaa tatttgtacc ttagcctact
     4441 tgatcatgtt ggcttatggt ctgattcgct tacggaaaga aaaaggaatg cccaaagctg
     4501 gggaatttaa aacaccattg gtacctctgt taccaatttt atcgatcatc atttgtttat
     4561 ccttcatgtt acagtataat atgaatacct ggattgcttt cctagttgcg ttgttagtag
     4621 gaagtattat ttacttcact tatggctata agcattctac catagaagaa taaagtgaaa
     4681 gtctattgag ttgttgacac tcagcagatt tttatatgtg aaagaaattt caaatttttt
     4741 ctaaaaatat cttgacagat taaattttca ggagtagaat atttactagt taattaaatg
     4801 gttaaggagt tgttcatgaa gaaacaaaat ttatttttag tcctgttaag tgtctttctt
     4861 ttgtgcttgg gggcttgtgg tcaaaaggaa agtcagacag gaaaggggat gaaaattgtg
     4921 accagttttt atcctatcta cgctatggtt aaggaagtat ctggtgactt gaatgatgtt
     4981 cggatgattc agtcaagtag tggtattcac tcctttgaac cttcggcaaa tgatatcgca
     5041 gccatctatg atgcagatgt ctttgtttac cattctcata cactcgaatc ttgggcagga
     5101 agtctggatc ccaatctaaa aaaatccaaa gtgaaggtct tagaggcttc tgagggaatg
     5161 accttggaac gtgtccctgg actagaggat gtggaagcag gggatggagt tgatgaaaaa
     5221 acgctctatg accctcacac atggctagat cctgaaaaag ctggagaaga agcccaaatt
     5281 atcgctgata aactttcaga ggtggatagt gagcataaag agacttatca aaaaaatgcg
     5341 caagccttta tcaaaaaagc tcaggaattg actaagaaat tccaaccaaa atttgaaaaa
     5401 gcgactcaga aaacatttgt aacacaacat acagcctttt cttatctagc gaagagattt
     5461 gggcttaatc aacttggtat tgcaggcatc tctcctgaac aagaaccaag tccacgacaa
     5521 ttagcagaaa ttcaggaatt tgttaaaacc tataaggtta aaacgatttt tacagaaagt
     5581 aatgcttctt caaaagtagc tgaaactctt gtcaaatcaa caggtgtggg tcttaaaact
     5641 ctgaatcctt tagaggcaga cccacaaaat gacaagacct atttagaaaa tctggaagaa
     5701 aatatgagtg ttctagcaga agaattaaag tgaggaaaga atgaaaatca ataaaaaata
     5761 tctagcaggt tcggtggcag ttcttgccct aagtgtttgt tcctatgagc ttggacgtta
     5821 ccaagctggt caggataaga aagagtctaa tcgagttgct tatatagatg gtgatcaggc
     5881 tggtcaaaag gcagaaaact tgacaccaga tgaagtcagt aagagggagg ggatcaacgc
     5941 cgaacaaatc gtcatcaaga ttacggatca aggttatgtg acctctcatg gagaccatta
     6001 tcattactat aatggcaagg tcccttatga tgccatcatc agtgaagagc tcctcatgaa
     6061 agatccgaat tatcagttga aggattcaga cattgtcaat gaaatcaagg gtggttatgt
     6121 ccttaaggta aacggtaaat actatgttta ccttaaggat gcagctcatg cggataatat
     6181 tcggacaaaa gaagagatta aacgtcagaa gcaggaacgc agtcataatc ataactcaag
     6241 agcagataat gctgttgctg cagccagagc ccaaggacgt tatacaacgg atgatgggta
     6301 tatcttcaat gcatctgata tcattgaaga tacgggcgat gcctatatcg ttcctcacgg
     6361 cgaccattac cattacattc ctaagaatga gttatcagct agcgagttag ctgctgccca
     6421 agcctattgg aatgggaagc agggatctcg tccttcttca agttctagtt ataatgcaaa
     6481 tacagctcaa ccaagattgt cagagaacca caatctgact gtcactccaa cttatcatca
     6541 aaatcaaggg gaaaacattt caagcctttt acgtgaattg tatgctaaac ccttatcaga
     6601 acgccatgtg gaatctgatg gccttatttt cgacccagcg caaatcacaa gtcgaaccgc
     6661 cagaggtgta gctgtccctc atggtaacca ttaccacttt atcccttatg aacaaatgtc
     6721 tgaattggaa aaacgaattg ctcgtattat tccccttcgt tatcgttcaa accattgggt
     6781 accagattca agaccagaac aaccaagtcc acaatcgact ccggaaccta gtccaagtct
     6841 gcaacctgca ccaaatcctc aaccagctcc aagcaatcca attgatgaga aattggtcaa
     6901 agaagctgtt cgaaaagtag gcgatggtta tgtctttgag gagaatggag tttctcgtta
     6961 tatcccagcc aaggatcttt cagcagaaac agcagcaggc attgatagca aactggccaa
     7021 gcaggaaagt ttatctcata agctaggagc taagaaaact gacctcccat ctagtgatag
     7081 agaattttac aataaggctt atgacttact agcaagaatt caccaagatt tacttgataa
     7141 taaaggtcga caagttgatt ttgaggcttt ggataacctg ttggaacgac tcaaggatat
     7201 ctcaagtgat aaagtcaagt tagtggaaga tattcttgcc ttcttagctc cgattcgtca
     7261 tccagaacgt ttaggaaaac caaat
//
LOCUS       DAVLXF010000093         6855 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLXF010000093 DAVLXF010000000
VERSION     DAVLXF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.093"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            158..1387
                     /gene="arcA"
                     /locus_tag="VYO12_001818"
     CDS             158..1387
                     /gene="arcA"
                     /locus_tag="VYO12_001818"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1588367.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1445..2461
                     /gene="argF"
                     /locus_tag="VYO12_001819"
     CDS             1445..2461
                     /gene="argF"
                     /locus_tag="VYO12_001819"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1588368.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2626..3573
                     /gene="arcC"
                     /locus_tag="VYO12_001820"
     CDS             2626..3573
                     /gene="arcC"
                     /locus_tag="VYO12_001820"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1588369.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3782..5293
                     /locus_tag="VYO12_001821"
     CDS             3782..5293
                     /locus_tag="VYO12_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1588370.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5315..6646
                     /locus_tag="VYO12_001822"
     CDS             5315..6646
                     /locus_tag="VYO12_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1588371.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLTDKE
                     ELVELLTRCAQNYQLRYEEFDYLASLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         2047 a         1380 c         1444 g         1984 t
ORIGIN      
        1 tgttatgtat aataacataa aattgtgcta aacatcataa tttatattat tttccgagaa
       61 attatagcca ttgacattac ctaggaattg cgttataaca tacatgtaag cggtacccaa
      121 aatggtgcca agtcaaaatt tttaaggagg aaaatacatg tcttcacatc caattcaggt
      181 cttctcagaa attgggaaac tgaaaaaagt tatgttgcac cgtccaggca aggagttaga
      241 aaacttgttg ccggactatc ttgaaaggct tctttttgat gatattcctt tcttggaaga
      301 tgctcaaaaa gaacatgatg catttgccca agctcttcgc gatgaaggaa ttgaggttct
      361 ctacctagaa caactcgctg ctgaatcatt gacctctcca gaaatccgcg atcaatttat
      421 cgaggaatac ttagacgaag ccaacatccg tgatcgtcaa accaaggttg ctattcgtga
      481 attgcttcac ggcatcaagg acaaccaaga attggttgaa aaaacaatgg ctgggattca
      541 aaaagttgaa ttgccagaaa ttcctgacga agctaaagat ctaactgact tagttgaatc
      601 agattatcca tttgcaattg acccgatgcc aaacctctat ttcactcgcg acccatttgc
      661 aacaattgga aacgccgtat cgcttaacca catgtttgca gacactcgta accgtgaaac
      721 actctacggt aagtatatct tcaaatacca cccaatctat ggcggaaaag tggatttggt
      781 ctacaaccgt gaagaagata cgcgtatcga aggtggagac gagttagttc tttctaaaga
      841 cgtccttgca gtaggtatct ctcaacgtac agacgcagct tctatcgaaa aacttttggt
      901 caacatcttc aagaaaaatg ttggcttcaa gaaagttttg gcctttgaat ttgctaacaa
      961 ccgtaaattc atgcacttgg atactgtctt cactatggta gactatgaca agttcactat
     1021 tcacccagaa atcgaaggcg accttcacgt ttactcagtt acttacgaaa acgaaaaact
     1081 taaaatcgtt gaagagaaag gtgacttagc tgaacttctt gctcaaaacc ttggtgtaga
     1141 aaaagttcat ttgattcgtt gcggtggtgg caatatcgta gcagctgcgc gtgaacaatg
     1201 gaacgacggt tctaacactt tgaccatcgc acctggtgtg gtagttgttt atgaccgcaa
     1261 taccgtgacc aataagattt tggaagaata cgggcttcgc ttgattaaga ttcgcggaag
     1321 tgaattggtt cggggccgtg gtggacctcg ttgtatgtct atgccatttg aacgtgaaga
     1381 agtgtaatcg ctgttcggca ttcgtcaata gaaaatgtaa aaaatagaaa gaggaaataa
     1441 taaaatgaca aattcagtat tccaaggacg cagcttctta gcagaaaaag actttacccg
     1501 tgcagagtta gaatacctta ttggtctttc agctcacttg aaagatttga aaaaacgcaa
     1561 tattcaacac cactaccttg ctggcaagaa tatcgctctc ctatttgaaa aaacatctac
     1621 tcgtactcgt gcagccttta caactgcggc tatcgacctt ggtgctcacc cagaatacct
     1681 cggagcaaat gatattcagt tgggtaaaaa agaatctact gaagatactg ctaaagtatt
     1741 gggacgtatg tttgacggga ttgaattccg cggattcagc caacgtatgg ttgaagaatt
     1801 ggcagaattc tcaggcgttc cagtatggaa cggtctaact gacgaatggc acccaactca
     1861 aatgctcgct gactacttga ctgttcaaga aaacttcggt cgcttggaag gcttgacatt
     1921 ggtatactgt ggtgatggac gtaacaacgt tgccaacagc ttgctcgtaa caggtgctat
     1981 ccttggtgtc aatgttcaca tcttctcacc aaaagaactc ttcccagaaa aagaaatcgt
     2041 tgaattagca gaaggatttg ctaaagaaag tggcgcacat gttctcatca ctgaagatgc
     2101 tgatgaagca gttaaagatg cagacgttct ttacacagac gtttgggtat caatgggtga
     2161 agaagacaaa ttcgcagaac gtgtagctct tcttaaacct taccaagtca atatggactt
     2221 agttaaaaaa gcaggcaatg aaaacttgat cttcctacac tgcttgccag cattccacga
     2281 tactcacact gtttatggta aagacgttgc tgaaaaattt ggtgtagaag aaatggaagt
     2341 aacagacgaa gtcttccgca gcaagtacgc tcgccacttc gatcaagcag aaaaccgtat
     2401 gcacactatc aaagctgtta tggctgctac acttggtaac ctttatattc ctaaagtata
     2461 attttagata ataaaccgtc taccaacagc tatgagggct gcgactaata gctttagtcc
     2521 ggtcctcttt tatataatgg taatctatta tttcttataa aatatgtgaa aaatcattaa
     2581 attgaaatct aaacgcattc tattgagtgt gataaaggag aatttatggc aaatcgtaaa
     2641 attgtagtag ctttgggagg aaatgcgatt ctttcttctg acccatcagc aaaggctcaa
     2701 caagaagctt tagttgaaac agctaagcat cttgtaaaat tgattaaaaa tggagatgat
     2761 ctgattatca ctcacggtaa tggacctcaa gttgggaatc tcttgctcca acatttggca
     2821 tcagactctg aaaagaaccc tgccttccca ctcgactcac ttgtcgctat gacagaaggt
     2881 agcatcggtt tctggttgaa aaatgctttg caaaatgctc tcttggatga aggcatcgaa
     2941 aaaaatgttg cctctgttgt aacgcaagtt gtcgtagata aaaatgatcc agcttttgtt
     3001 aacttgagta aaccaatcgg tcctttctat tcagaagaag aagcaaaagc agaagccgaa
     3061 aagagcggag cgactttcaa ggaagatgct ggccgtggct ggcgtaaggt cgttgcctca
     3121 ccaaaacctg ttgacatcaa agaaattgaa accatccgta ctcttttaaa taatggtcaa
     3181 gtcgtcgtag ctgcaggtgg tggcggtatt cccgtcgtca aagaaaacaa tggacatttg
     3241 actggtgtcg aagcggttat tgataaagac ttcgcttccc aacgtttggc agaattggtt
     3301 gatgcagacc tcttcatcgt tttgacaggt gtagattatg tatttgttaa ctacaacaag
     3361 ccaaaccagg aaaaattgga acatgtgaat gttgcccagc tggaagaata tatcaaacaa
     3421 gatcagtttg caccaggtag catgcttcca aaagtagaag cagctatcgc ttttgtcaat
     3481 ggtcgtccag aaggaaaagc agttattact tcccttgaaa atctaggcgc cttgattgaa
     3541 tctgaaagcg gaacaattat tgaaaaagga taagttgttt tactaataag atgtattcta
     3601 tttctagtat ctttatatca aattagaaat tattcttgaa aacatgtaca atatttcaaa
     3661 agatactagt tttagacttt aatatggtaa aacaaatata aatagaaagc gttttcttga
     3721 atgtttattt aagaaagtag ttggtttttt acactttgtt agacatcagg aggaaaaaca
     3781 aatgagtgaa aaagctaaaa aagggtttaa gatgccttca tcttacaccg tattattgat
     3841 aatcattgct attatggcag tgctaacttg gtttatccct gcgggggcct ttatagaagg
     3901 tatttacgag actcagcctc aaaatccaca agggatttgg gatgtcctca tggcaccgat
     3961 tcgggctatg ctaggtactc atccagagga aggttcgctc attaaagaaa cgagcgcagc
     4021 gattgatgta gccttcttca tccttatggt tggtggtttc cttggcattg tcaacaaaac
     4081 tggtgctctt gacgtaggga ttgcctctat cgtgaagaag tataagggcc gcgaaaaaat
     4141 gttaattttg gtactgatgc ctttgtttgc cctcggtggt acaacttatg gtatgggtga
     4201 agaaacaatg gccttctatc cactccttgt gccagttatg atggccgttg gttttgatag
     4261 cctgactggt gttgcaatta ttttgctcgg ttctcaaatc ggctgtttag catctactct
     4321 gaatccattt gcgacaggta ttgcttcagc gactgcggga gttggtacag gggacggtat
     4381 cgtacttcgt ctgatcttct gggttacctt gactgctctt agtacttggt ttgtttaccg
     4441 ttatgcggat aagattcaaa aagatccgac taagtcactg gtttatagta ctcgcaaaga
     4501 agatttgaaa cactttaacg tagaagaatc ttcatctgta gaatctacac ttagcagcaa
     4561 acaaaaatca gttctcttct tatttgtgtt gacattcatc ttgatggtat tgagcttcat
     4621 tccatggaca gaccttggcg ttaccatttt tgatgacttt aatgcttggt tgactggtct
     4681 tccagttatt ggtaatattg tcggttcatc tacttctgca ctaggtactt ggtacttccc
     4741 agaaggcgca atgctctttg cctttatggg tatcctgatt ggtgttattt atggtcttaa
     4801 agaagataag attatctctt ccttcatgaa tggtgctgct gacttgctca gtgttgcctt
     4861 gatcgtagcg attgctcgtg gtattcaagt tatcatgaac gacggtatga ttaccgatac
     4921 aatcctcaac tggggtaaag aaggcttgag cggtctatct tcacaagtct ttatcgttgt
     4981 aacttatatc ttctatctac ctatgtcatt cttgatccca tcttcatctg gtcttgccag
     5041 cgcaactatg ggtatcatgg ctccacttgg agaatttgta aatgtccgtc ctagcttgat
     5101 tatcactgct taccaatctg cttcaggtgt cttgaacttg attgcaccaa catctggtat
     5161 tgtgatggga gctcttgcac ttggacgtat caacattggt acttggtgga aattcatggg
     5221 caaactcgta gtcgctatta ttgtagtgac catcgccctt cttctccttg gaaccttcct
     5281 tccattccta taaaatagtg agtgaggtga ttccatgaaa atagatataa caaatcaagt
     5341 taaagatgaa tttcttatat cattaaaaac cttgatttcc tatccttcag tactcaatga
     5401 aggagaaaat ggaacacctt ttggacaagc aatccaagat gtcctagaaa aaactttaga
     5461 gatttgtcga gacataggtt tcactaccta tcttgaccct aaaggttatt acggatatgc
     5521 agaaatcggt cagggagcag agcttctggc cattctctgt catttggatg ttgttccatc
     5581 aggtgatgaa gcagattggc agacaccgcc atttgaagca actatcaaag acggctgggt
     5641 attcggacgt ggtgtccaag atgataaagg cccttcgctc gcagctctct atgcagtaaa
     5701 aagcttgctg gaccaaggta ttcagttcaa aaagcgcgta cgctttatct ttggtaccga
     5761 tgaggaaacc ctctggcgct gcatggcacg ctacaatacc atcgaagaac aggccagcat
     5821 gggctttgca cctgactcat cttttcctct gacctatgct gaaaaagggc ttctacaggt
     5881 caaacttcat ggccctggat cggatcaact agagcttgaa gtaggaggcg cctttaacgt
     5941 tgtaccagac aaggccaact accaaggtcc cctctatgaa caggtttgta acgatctcaa
     6001 agaagctggt tatgattacc aatccactga acaaaccgta acggttctcg gagtgccaaa
     6061 gcatgctaag gatgctagtc aaggtatcaa tgctgtcatc cgactagcta ccattcttgc
     6121 tcctctccaa gaacaccctg ctctcagctt tcttgcaaca caagcaggtc aagacggcac
     6181 aggaagacaa atctttggtg atatagcaga tgaaccttct ggtcacctat cctttaatgt
     6241 cgcaggtctc atgatcaatc atgaacgttc tgaaatccgt attgacattc ggactcctgt
     6301 cttaactgat aaggaagaac tagtagagtt gcttacaaga tgtgcacaaa actaccaact
     6361 ccgctacgaa gagtttgact atctagcgtc tctatacgtc gcaaaagaca gtaaactcgt
     6421 tagcacactg atgcaaatct accaagaaaa gactggcgat aacagtcctg ctatttcatc
     6481 cggtggtgcc acttttgctc gcaccatgcc aaattgtgta gccttcggcg ccttattccc
     6541 aggagcgaag cagacagaac atcaggcaaa tgaatgtgcc gttctagaag atttgtaccg
     6601 tgctatggat atttatgccg aagccgtcta tcgacttgca acttaatcag gcaactgttt
     6661 ctaccaaaaa aaatcccgca aaatgaactg caccccaaaa gttagacaga ataaatctaa
     6721 cttttggggt gttttattat gaaattgagt tatgaagata aagttcagat ctatgaacta
     6781 agaaagcaag gacaaagctt caaacagctt tcaaaaagat ttggtgtgga tgtttctggt
     6841 ctaaagtcat ctgaa
//
LOCUS       DAVLXF010000094         6765 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLXF010000094 DAVLXF010000000
VERSION     DAVLXF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.094"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            62..135
                     /locus_tag="VYO12_001823"
     tRNA            62..135
                     /locus_tag="VYO12_001823"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Asn,seq:gtt)
     gene            complement(176..718)
                     /locus_tag="VYO12_001824"
     CDS             complement(176..718)
                     /locus_tag="VYO12_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1588372.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            896..1663
                     /locus_tag="VYO12_001825"
     CDS             896..1663
                     /locus_tag="VYO12_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1588373.1"
                     /translation="MIGISLIPALYNIIFLSSMWDPYGQLSDLSVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSKKTTTLSNI
                     QSTAAYQSLTSEQQTGISDSVSQNSTDSIQSAQSIVALVQGLQGSLENLQNQSSNLST
                     LKNQANQVLPITSTSLIGLSSGLTEIQGAVTSKLVPASQLIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            1748..2155
                     /locus_tag="VYO12_001826"
     CDS             1748..2155
                     /locus_tag="VYO12_001826"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588374.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNIIFAKLPSGRH
                     PESRWPWLKSRAEINGIIAVLAGILVYGGVLLV"
     gene            complement(2525..5077)
                     /locus_tag="VYO12_001827"
     CDS             complement(2525..5077)
                     /locus_tag="VYO12_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1588375.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQALKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISITSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTYKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(5139..6761)
                     /locus_tag="VYO12_001828"
     CDS             complement(5139..6761)
                     /locus_tag="VYO12_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1588376.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         2229 a         1113 c         1249 g         2174 t
ORIGIN      
        1 ggttaagaca ccgccttttc acggcggtaa cacgggttcg aatcccgtac ggactatttt
       61 atccgccata gctcagttgg tagtagcgca tgactgttaa tcatgatgtc gtaggttcga
      121 gtcctactgg cggagtatag ataaaaggga acacagctgt gttcctcttt ttgtatcaat
      181 ttgtatcacc aagcattttc ataaggaagt ctgttatttc ttgagaactt tctttttttc
      241 catgtgcaat ccaagtttgg cagacaccaa aaagtgcatg agttagatag atgctactat
      301 attctaattc agtggtattt agattcagtt gcataaatcg cttttgtaaa tctgtactaa
      361 gcatgatatg aagtttattt cgtaagaaat tttggatttc tttagtccca ttttcagaaa
      421 gaagggcagc cagaagtggt tctgactcta gatattcaaa aacttctaaa atagcgtctc
      481 ttttgtgatg agcatgtttt tgaaaaatat attcaaatgt atggaatagc ttgctttgat
      541 agtgctcaat catatcatac ttatccttat agtgagtata gaagctggaa cgactaattc
      601 cggctttttc tactaatttg acagtagaaa ttttatcaaa tggctgttcc atcagtaatt
      661 gtaccatagc attttcaata gttcgctttg tttttaagcg tttgttactt tcttgcatat
      721 ttcctccttg taaataaatt agactatatg tctaaaaata gattttttat cttgtaattt
      781 agatttttta atgtataatc tattatatca aaattttaga caatatgttt aaaaaaggag
      841 aaactatgtt taaagaatgg aaagcaattt aaaaaaaaca acctttatta ttgtcatgat
      901 cgggatttct cttattccag ctctgtacaa tatcatattt ttgtcatcaa tgtgggatcc
      961 atatgggcaa ttgtctgact tatctgtggc agttgtaaat aatgataaag aggcttccta
     1021 taatggtaat actatggcaa taggaaaaga catggtgtcc aatttaaaag aaaataaaac
     1081 cttggatttt cattttgtag atgaagagga aggaaagaag ggattggaag atggcgatta
     1141 ctatatggta gtgactttac caagtgattt atctaaaaaa acaactacat tatccaatat
     1201 tcaatcgaca gcagcttatc aatcattgac aagtgagcaa caaactggga taagtgattc
     1261 tgtatctcaa aattcaactg atagtattca atcggctcag tcaattgtag ctttagtaca
     1321 aggtttacag ggaagtttag aaaacttaca aaatcaatct tctaatcttt cgactttaaa
     1381 aaatcaagct aatcaagtat tacctattac ttctacttct ttgataggat tgtcaagtgg
     1441 attaacagag atacaaggag ctgttactag caaattagtt cctgccagtc agttgattgc
     1501 atcaggtgta aacgcatata ctacaggtgt tgataaagtt tctcagggcg caagtcaact
     1561 aagtgaaaaa aatgccacct tgacaggtag tttggataaa ctagtttcag gctcaaacac
     1621 cttgacacaa aaatcttcta gattgacagc aggagttggt taattacaat caggatctgg
     1681 gcaattagca gacaaatcca gtcagttact ttcaggtgct tctccattag agaatagagc
     1741 taataaattg gcagatggat ctgggaaact agcagaaggt ggaacaaagt taacttctgg
     1801 attggaagat ttacagacag gacttgcttc tttaggacaa ggactaggta atgctagtga
     1861 tcaactcaaa tcagtatcaa cagaatctaa aaatgcagag attttgtcaa atccactcaa
     1921 tctttcaaaa acagacaatg atcaagttcc tgtaaatgga atcgcaatag ctccttatat
     1981 gatatcagtt gctctttttg ttgcagcaat atcaacaaat ataatatttg cgaaattgcc
     2041 ttcaggacgt catccagaga gccgttggcc ttggttgaaa tctcgagctg aaataaatgg
     2101 tattatagct gttttggcag gaattttggt atatggagga gttctattgg tttaactgct
     2161 aatcatgaga tgagaatatt tattctcatc atccaaacaa gtttagtatt catgtctatg
     2221 gtgaccactt tagcaacgtg gaatagccgt ataggagctt ttttctcact tattttgctt
     2281 ttactatagt tagcatcaag tgcaggtact tatccacttg ctttgacaaa tgatttcttt
     2341 agatctatta atccctgttt accaatgagc tattcagttt cgggattacg acaaacaatc
     2401 tctatcaaca agtcattttc ctagctgtca tactagctct atttactggt ttaggtatgc
     2461 tagcctatca acataagaaa atggaagaag attaaaaaaa tcgaccgatt aactggtcga
     2521 ttttttatgc cttagatgac tttcgtctgt gattatagat tccaaatagt aagagagaag
     2581 taaaggaaca gattgctcca gtaataaaac cattgggaat gaaggaaagt gtaatagttc
     2641 ctttcccctt gggaatgtca actttcataa atccagtttg agcttgttta atttctattt
     2701 tcttaccatc ttggtaggca gaccaacctt tgtcataagg aatggtgaag aaaatagatg
     2761 tatcttgttg gacatcatat gtagcaaaaa ccttgttttt ataagttgat actgtgacag
     2821 gttgttcttt aattttttga attgcctcgg tgaaagtttt ggtatctaaa cgatagaagg
     2881 taggagattc aaatgatact tgtgaatttc cagggaaact aacattgata ttgaaagttt
     2941 ttttctcttt agtatatcct agattaaaga aggagaagac attatcagtt gtaaaagtct
     3001 ttttttcacc atttacaagg atgtcaacct tcttttgttt atcgttagaa aagtgaaggt
     3061 ttatgaaaga gagataaact tggctgtttt ctggaacttc aatttgatac tggattgctg
     3121 catcttcatt tgaagaactt gtgatactaa tcaaatcatt agtattttct attttttctg
     3181 ttttttcata aggtattgga gaaaaataat caaaattgac gttagcaagt tgatttaaaa
     3241 atgaggcctg attatccaag gtatgttcat tgaacttgac atcattgtaa acagattgac
     3301 tagcaactgc aatcggaaga gagtattgat tttcatatag ggtaagatta tctttttgat
     3361 agatatcttt aaagccatac ttatcaatag gactgtctga gatattgtac tggataccaa
     3421 ataaactatc agccaaaata ctattatttg catatcggag attgagatta gtcccagagg
     3481 atttaaaacc aagtttatct aaagtagagc ttgatgaacg atttcgaaca gatgaaaatt
     3541 gagagattcc attgtagttg aatttcatac tgtcatttcc tgtctgagtt tgtagttttt
     3601 cagtacgagt aaattgattt ccaatatatg ttgagaaaga ttccatagct gggatatctc
     3661 gactataagc acttcgagaa gcaaatcccc attccttagc aattccgtcc atttgagatg
     3721 aagcatttaa actcatttca accagtataa ataaagagat tagaatggca aatagattca
     3781 cagatataaa ctttttgata actgcaagga gtaaaagaga atagacaacc aaaaattcaa
     3841 gagtaagcag aatattcaaa tctgttaaaa aagaataatg cgattttaga tagatggtag
     3901 ctaaaaatcc tgctactaca agaaaaagcg aaactaaaaa attccagact ttaagttctt
     3961 tcagacgctt taagacttct gctgctgtgt aaattaacaa ggtagagaaa atccaagcat
     4021 agcgatgtaa aaacatgttt ggagtatgca tgccttgcca aaataagtca agagcttcta
     4081 tgtaaaagct tgcaattaga aatgcaaaga atattacata tatgagtttc acgtgaaact
     4141 taatagattt cagcgtaaaa aataaaatgg tcaaaataaa gggaaatagt ccaacaaaaa
     4201 tcattgggat ggccccatac tttgttgtgt caaaggaacc aatgaattgc ttagcaaaga
     4261 gatcaagata ccagctactt tcagtttgaa actttgtaac ttcagtcaat ttttccccat
     4321 gtgtctgtaa atcaaataga gtgggaagag tcataatcaa actagccata ccagctaaaa
     4381 aggagataac tatgaaatca agaacagatg attttcgagt cttaaagtcc cacgaaattt
     4441 gacagagata ccagaaaata agaaacaata ctgtcatata tccaaaataa taattttgaa
     4501 taaataagat tgacagactt gtaaagtaca ataggagttt cttttcagtt atcagtagat
     4561 gtaaaccagt tataattaaa ggaatcaaga taaaaacatc tagccaggtt tttatctcta
     4621 attgactgac agtgaaactc atcagagcat aggaagtaga taaggctagt tttaaagcct
     4681 gagggataga ttgaaacaat ttattcaaac taaaaaaggt tgacagacca atcaatccaa
     4741 attttaagag agttgtcaga tagatagcat ctggcatatt cgttagatca aaaaagtaaa
     4801 ccagaggcgc gagaaaacta cccaagtaat aactagatag ggcatagaag tttagcccta
     4861 gaccacttgt aaaggtgtaa aacagactac tatttccatg taggatattt cgtaaggcta
     4921 catcaaaaat aacgtattga tgaaagccat ctcctaatag aggagagttg tcgctattcc
     4981 agtagatacc ttgagataga tatactccag acataatcac tacaggaatg atgaaagaaa
     5041 taaaataggt tcgatatgtt tttaaaaatg atttcatgtt acctcgtaga atgatagaaa
     5101 actcagttgg ttaacccaac tgagttttga agttttattt agtctttcca aagttcttta
     5161 acttttgctt gtacttctgc attttctagg aattcatcgt aggtttcatc gatacggtca
     5221 atgacgccat ttttagataa gacaatgata tggttagcca aagtttgaat aaattcgtgg
     5281 tcatggctgg caaagatgat tgattcttta aagtttttca atccatcatt caagcttgag
     5341 atagattcca agtccaagtg atttgttgga tcatcaagta caaggacatt tgattttaag
     5401 agcatgagtt ttgaaagcat gacacgaact ttttctcccc ctgacaagac atttacaggt
     5461 ttgttaactt catctccaga gaagagcata cggccgagga agccacgtag gaaagtattg
     5521 tcatcttctt ctttacttgc gaattgacgc aaccagtcaa gaattgattc tcctcctgca
     5581 aaatcagctg agttatcttt tggtaggtaa gattgactag ttgtaactcc ccacttgaca
     5641 gttccttcat agtcaatatc tcccatgatt gcacgaatta atgcagtcgt ttgaatatca
     5701 ttttgtccaa taagtgctgt cttatcatct ggacgcaaga tgaaactaat attatccaag
     5761 atagtttcac catcaatctt tacagttaga ttttctactg tcaagagatc attaccaatc
     5821 tcacgttccg ctttaaagtt gataaatgga tatttacgac tagatggtac aatctcttct
     5881 agctcaatct tatcaagcat tttcttacgt gatgttgcct gccttgactt agaagcattg
     5941 gcagagaaac gagcaacgaa ttcttgcaat tgtttaattt tttcttctgc tttagcatta
     6001 cggtctgcta gcaatttagc agcaagctca gaagattcct tccagaagtc gtagtttccg
     6061 acatagagtt tgatttttcc aaagtcaagg tcggccatgt gagtacaaac tttgtttaag
     6121 aagtgacggt cgtgggatac tacgataact gtgttatcaa agtcaatcaa gaagtcttct
     6181 aaccaagtaa tcgattggat atccaaaccg ttagtaggct cgtccaagag aagaacatct
     6241 ggtttaccaa aaagtgcttt ggcgaggaga acctttactt tttcaccgtt ggccaattcg
     6301 ctcatgtttt ggtagtgtaa ttcttctgga atgtttaggt tttgaagtag ttgagaggct
     6361 tcactctctg cttcccaacc tccaagttcg gcaaactctc cttcgagttc ggcagcacga
     6421 accccgtcct cgtctgagaa atcttccttc atgtagatag catctttctc tttcatgatg
     6481 ctataaagtt tttcatttcc catgataacg acatcaatgg cacgttcatc ttcgtagtca
     6541 aagtgatttt gacgaagaac agagagacgt tcatctggac caagagagat gtgaccagta
     6601 gtaggttcga tatctccagc taaaattttt aaaaaggttg attttccggc accattagca
     6661 ccgattaatc cgtaagtatt tccttctgta aatttgatat tgacatcatc aaaaagtttg
     6721 cgatcactaa aacgtagtga aacatcagat actgtaagca atatt
//
LOCUS       DAVLXF010000095         6718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLXF010000095 DAVLXF010000000
VERSION     DAVLXF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.095"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(40..1734)
                     /locus_tag="VYO12_001829"
                     /pseudo
     CDS             complement(40..1734)
                     /locus_tag="VYO12_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(1837..2822)
                     /locus_tag="VYO12_001830"
                     /pseudo
     CDS             complement(1837..2822)
                     /locus_tag="VYO12_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            complement(2925..3068)
                     /locus_tag="VYO12_001831"
     CDS             complement(2925..3068)
                     /locus_tag="VYO12_001831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588377.1"
                     /translation="MTGQRLNELEINGIRLTKFKNGEIGIEFIWIDTENPPSDAIGWV
                     AKK"
     gene            complement(3108..3863)
                     /locus_tag="VYO12_001832"
     CDS             complement(3108..3863)
                     /locus_tag="VYO12_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1588378.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSLEDLY"
     gene            complement(3934..6585)
                     /locus_tag="VYO12_001833"
     CDS             complement(3934..6585)
                     /locus_tag="VYO12_001833"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1588379.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWDEFADWYVELTKEVLYSDNEEDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELDRLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(6597..>6718)
                     /locus_tag="VYO12_001834"
     CDS             complement(6597..>6718)
                     /locus_tag="VYO12_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1588380.1"
                     /translation="LKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
BASE COUNT         1992 a         1522 c         1199 g         2005 t
ORIGIN      
        1 gaaatgaaaa tcatacatag aaatttttaa aaattttctc tatcgtaaac ctgccactag
       61 ttcagaaact atactagctg attccaaacg taatatggtc atctcatcta ccttccctaa
      121 taatggcatt gcaccatacc aaacaatagt cttattcacc aaaagaatag gcgttactat
      181 ttttgattga gtaaaagaca ctttaatttt ctggctcttc aactggtcta gccatgcttt
      241 atttgcaacc gtctctggta tacatatctc aatttgacta atcctaaatt cttttagaaa
      301 attcatcagt tttgtctggt gcacataagg taaaataagc agacattcct gtctttcccc
      361 tgctaagtcc tctctcaaca ccttctcata tctactatca acataaacga attgtttctc
      421 ctcaccctca atggcacgat aatccatctt tcgataagct acttgtcgtt tctgaaacat
      481 cttttctaaa taaggaacat gaatatccac ataatcgaaa atacgcacca aagacttatc
      541 cttgtagttt ctatgaatcc aacctgcata ctgaatcaaa ttatttttcc aagaaaaggg
      601 tgctgccaag ataagcgtgt ccaactgagg taagtcaaaa ccttcgccaa tgtattttcc
      661 agtagacaac aaaacaaacc ctttatctaa ctgttctaac gtctccagta aactcgttct
      721 ctctcggact ttggtttttc cgctaataat gtaacagtca tcaacctctt tctctttcaa
      781 taatttttca aagacatcta tctgttgaat tcgattaact aaaaccaaga tattccgtcc
      841 ttctgccact tgggctagaa tatccttgag aatcatctga ttcctcactg agtcagtagc
      901 aatccaatca ctaagctgta taaaattact tgctttggtc ttttcaattt ccaaatgacc
      961 aaaagaagtg aatcttaatt gcaattgccg tttaaaatcc gtttccctct tatcagcagt
     1021 atggagtatc tcaccaattc tctgaaaaac aataggctca tgaccattct tacgctcagg
     1081 cgtagccgtc aaaccgtaaa gatacttccc tctaaactga gcaacaactt tttcaaacat
     1141 caaggcagag acatgatgac actcatccac aatcatcatc tcatactcat ccaaaagact
     1201 ttgactattt tccaacttaa atagagattg aatcataacg acatcaacca gtttactcag
     1261 ccatttctta gtcccaccgt actgcccaac atagccgatt accttttcac gacctgatgc
     1321 cgtataacgg atagcctcct cctcttcgaa agtcaaaaag cagtttaagc gatctagcca
     1381 ttggtctaag agttgcttat tatggactag aataattgtt tttgttttcc gttcagagat
     1441 aagagcagca cctaaaacgg tcttcccaaa accagtctcc gcatgaagta aaccattttc
     1501 tttagaagtc atatctgaca gggctaactc ttgctcaaaa gtgagttctc ccttaaattc
     1561 cacgctaata gacctttgta cctttctcct atcttccaca gatacctgct taaatttatc
     1621 ttgcaatgga tacagcaaac ctcttggcaa ccataaataa tgatcggatt ctccaaataa
     1681 atacattctc tcaggaattt gataggttgg ctgtcgcata gcctgcttta aatatagtaa
     1741 aatgaaataa taacaggaca aatcgatcag gaaagtcaaa tcgatttcta acaatgtttt
     1801 agaagcagag gtgtactatt ctagtttcaa tctactataa aattcgggat tagaaaagga
     1861 agccatattt ttcaagaaaa aagtgccttg gatgtcacag acgatttttc aagttggatc
     1921 atattggata aaacgacctt caactcctta tccaattctt gcttgcctaa ctcctcttgg
     1981 attaacagtg ccactttagc agttgaaatc ctctgaattt cttgtagata cctccattgg
     2041 tcttcataag gctgaaactg ttcatccaca aagaccgttc gcccttgatg gtaagcttct
     2101 ccttgaaaag gcaaggcaat caaatttcca aatcccccct taggaaggac atcctgattt
     2161 ggaaacatgc gatcaaaaga atcaaaggac agttgcatac tttcctgcat tgccaattct
     2221 aatagtttct ttccaaacag gcgagccttt cgactcagaa tcgcttcctc aaagaagaac
     2281 caaatatgga gtccgtaacc cgaacgagaa atctctaaat gggcttccat ctggcgttcc
     2341 ctggctattc ttcgaatggt taaaccagct tctttccaat ctccttcatc caaatccagt
     2401 accaaaaaat aacagctatc atctaagtgc ataggaaaga taccgatagc tgcctcccca
     2461 cgaaaatgag atttcaaaac ggaatccgtc aatgtctgga aactccgttt ttcaggtagc
     2521 aactgcttcc aaccataatc ataggaagga aaatactgaa ttttcccttg atcattgata
     2581 aaactcttag cataaacatc ataacggcca gcaaacacag aggtaaacaa ttgtaatttt
     2641 tcttgcgtcg tcaagcgact actttgaagt tggaacatct cctcgaactg agagcgttcc
     2701 ataacaaact gactcttgtc tttaagacgt ttacacaaga aaaactcact atccccatca
     2761 aaagaagaaa agacatcaag tacttctacc acaattccat ccaaagacac aaaaacagcc
     2821 ataagagtca cctccttgat tcctataggc tgattataac aagaatagct gaaattgtac
     2881 acgaaaataa aatcccttat ttgttacaat acctttgaaa acttttattt ctttgctacc
     2941 cagccgatgg catcgcttgg gggattttcg gtatcaatcc agataaattc aataccgatt
     3001 tccccatttt taaatttagt taaacgaata ccgttgattt ccagttcatt gagtcgttga
     3061 cctgtcaaga cttctcgttc tttcaactgg aaaagttaag gggggaacta atacaaatct
     3121 tctaagcttc tatattcaat gatttcattt ttgataatat tctttttaaa ctcaaaatgt
     3181 tttaaaggat tatcgattaa cactaacttt ggaatatcat ctagatttgt gaccaaggat
     3241 aaaataaaat ccgattgaaa ctccttgact ttttcaaatt cttttgcagt taaacgcaca
     3301 cgccccttag attttcggat accttttact tcggcaaggt aagaatactc ttggacatct
     3361 acctgaaagt catatccatc accataaaga cgggcatcgg ttaactgtcc accttggaat
     3421 ttttcttctt tatcaaaatg aaggataaaa taattctcag cctccattcc cgtttcctgt
     3481 agacgtttga atttagttcg aactattggt ttttcaacga tggttatgcc tgttttcttc
     3541 ccttcactgg caagtaacat tttaactatt tcagcataac tatgaacatc ttcatttcca
     3601 aacatcatat caattaaatc tttacgaaaa cgataaactt cagctttctg ccaccaacct
     3661 tttcgattat tgtcaaaata aggatcaaat aaatccattc tattttttac aactccagtt
     3721 gtttcagcaa ttcccagaga aaccacataa cgataaaact ccgacttgct agagcattga
     3781 aattctttta taaaaagctt gtcaaacttg gcaagaccgt agccaattaa atttaacaat
     3841 tcataatgag ggtgtttgga catcactttc tccatcaata gatattgatt tcattatacc
     3901 aaaaaaacac ggctcatcac cgtgtttctg tgtttattta acgagtttct tcatctcatc
     3961 aatacgtgct acggtcgcat catacttggc ttggtagtca gcttgtttgt cgcgttcttt
     4021 ttggacgact tctggtttgg cgttggctac gaagcgttcg ttagagagtt tcttaccaac
     4081 catgtccagt tctttttgcc atttagcgag ttctttgtcg agacgatcta gttcttcttc
     4141 aacattgagg agatcggcca gtggcaggta gatttctgct cctgtgatga cacttgacat
     4201 agccagttca ggtgcaggga tggttgatgc gatttccaaa tgttctggat ttgtaaagcg
     4261 tttgatgtag ttgacattgc tgttaaagaa ggcttccaag tcactatcgc tagtcttaac
     4321 aaggattgta ataggtttgc ttggtgctac atttacttcc gcacgcgcat tacgaacggc
     4381 acggatcaag tctttgagac tttctacacc agtgtgagcc gcaaggtctt caaaggctag
     4441 attaacagtt gggtatgcag ctgtcacgat agaaccttct gagatttgtc caaagatttc
     4501 ctctgtcacg aatggcatga ttgggtgaag gagacgaagg atcttgtcca gagtgtaaag
     4561 gagaacagat cgagtaatta ccttatcttc ttcattatcg ctataaagga cttccttggt
     4621 caactcaaca taccagtccg caaattcatc ccagatgaag ttgtagagga tgtggccagc
     4681 aacaccaaac tcaaacttat caaagttagc agttgctttt ccaatcgttt cattgaggtt
     4741 gtggagaatc cagcggtccg tcacattacc agcctcacct gttgcaactt ttgtgacatt
     4801 gtcatgcgcc acatccagcg tcaaacctcc attgttcatg aggatatagc gagaaatgtt
     4861 ccaaatcttg ttaataaagt tccatgaagc atccattttc tcataagaga aacgcacgtc
     4921 ttgtcctggc gcagaaccgt ttgaaaggaa ccatcgaagg gcatcggcac cgtatttctc
     4981 gataacatcc attgggtcaa tcccgttacc gagagactta gacatcttgc gtccttgctc
     5041 gtcacgaata agaccgtgga taaggacgtt ttggaatggt tgacggcctg tgaattccaa
     5101 tgactggaag atcatacgag acacccagaa gaagatgatg tcgtaacctg ttaccaaggt
     5161 tgaagttggg aaataacgtt taaagtcttc tgagtcgact tcaggccagc ccatggttga
     5221 aaatggccag agggcagaac tgaaccaagt atccaagacg tcttcgtcct gagtccatcc
     5281 gtcaccttct ggagcttctt cgccgacata catttcacca tcagcattgt accaggcagg
     5341 gatttggtga ccccaccaaa gctgacgaga gataacccag tcgtggacat tttccatcca
     5401 ttgaaggaag gtatcgttga aacgaggtgg gtagaattcg accttgtcct ctgtgtcttg
     5461 gttggcaatg gcgttcttag ccaattggtc catcttgacg aaccattgag tagacaagcg
     5521 tggctcaact acgacacctg tacgttctga gtgaccaaca ctgtggacac gtttttcgat
     5581 tttgacaagg gcaccgattt cttccaactt agcaacgact gccttacgag cttcaaaacg
     5641 atccatgcct gaaaattcaa aggcaagctc attcatagtt ccgtcgtcgt tcatgacgtt
     5701 gacttgtggc aagttatgac gttggccaac caagaagtca tttggatcgt gggcaggtgt
     5761 gattttcacg acaccagtac caaactcagg atctgcgtgc tcatctccaa cgattgggat
     5821 gagtttatta gcgattggaa ggatgacgtt tttaccaatc aagtccttgt agcgcgggtc
     5881 ttctggatta accgcaaccg caacgtcccc aaacatagtc tcaggacgag ttgtagcaac
     5941 ttcaagggcg cgtgaaccat cttccagcat gtaattcatg tggtagaagg caccttccac
     6001 atccttgtga atcacctcaa tatcagaaag ggctgtgcga gctgctgggt cccagttgat
     6061 gataaactca ccacggtaga tccagccttt cttgtaaagg ttcacaaaga ccttacgaac
     6121 agcttttgac aaaccttcgt caagagtgaa acgctcacga gaatagtcta cagagagccc
     6181 catcttgccc cattgttcct tgatggtagt ggcatattcg tctttccatt cccagacttt
     6241 cgtcaagaaa gactcacgac caaggtcata gcgggaaatg ccctcaccac gcaagcgctc
     6301 ctcaacctta gcctgagtgg caatccctgc gtggtccatc ccaggaagcc aaagggtatc
     6361 aaaaccttgc atgcgttttt gacggatgat aatatcttgc aaagttgtat cccaagcgtg
     6421 accaaggtga agtttacctg taacgtttgg tggtggaata acgattgaat aaggcttagc
     6481 cttttgatcg cctgaaggct tgaaaacatc ggcatcaagc catttttggt aacgaccagc
     6541 ctcaacctcg gctggattgt atttagatga aagttcttta gacatgtgtg ttctccttaa
     6601 ttacttttat tgacaatttt aaccaaacca atttttgaaa gttcaatata aatctcttca
     6661 atttcagcaa atgctgcaag tggtgacata gtataatttc caggattata tttaagat
//
LOCUS       DAVLXF010000096         6650 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLXF010000096 DAVLXF010000000
VERSION     DAVLXF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.096"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(228..1316)
                     /locus_tag="VYO12_001835"
     CDS             complement(228..1316)
                     /locus_tag="VYO12_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1588381.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTIKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1334..2002)
                     /locus_tag="VYO12_001836"
     CDS             complement(1334..2002)
                     /locus_tag="VYO12_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1588382.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(2021..4459)
                     /locus_tag="VYO12_001837"
     CDS             complement(2021..4459)
                     /locus_tag="VYO12_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1588383.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPVTTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(4610..5908)
                     /locus_tag="VYO12_001838"
     CDS             complement(4610..5908)
                     /locus_tag="VYO12_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1588384.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            complement(5925..6233)
                     /locus_tag="VYO12_001839"
     CDS             complement(5925..6233)
                     /locus_tag="VYO12_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1588385.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(6279..6599)
                     /locus_tag="VYO12_001840"
     CDS             complement(6279..6599)
                     /locus_tag="VYO12_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1588386.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
BASE COUNT         2165 a         1380 c          976 g         2129 t
ORIGIN      
        1 taattaatgt attcacaata aatagccgac aacctatgtc gctttttgat ttatgaagaa
       61 cgtatagtag actgaatctg aaatagtacg aaacaattgc taaaacactt atagaaatta
      121 attttacttt cccaatcgat ttgttctcat cttatttcaa tctgctatag aattcaatac
      181 gaggatcaat aaaagactac catttggaaa acagtagtcc ttattgttta ttttgaattt
      241 acataggcat ctacagcgat aatagcttga gcaacatctg aaggcgaaat cttaaacggc
      301 atctgatgaa ttgtctcacc ctccatagtt gcttgtttac caacttttat taaatcatca
      361 tatccaactt gatccaaatg catttctttt atagttgttg gcataccaat ttttttgtaa
      421 aactcaatat acttatcaag ttcttcttta ggtctatttt ccaataatag ttgtactaaa
      481 gttccataag ctactttttc accatgtgtt aaatgatgaa tgtcacctgt caatgcagta
      541 aaaccattat gaattgcatg cgccgcagct aatcctccac tttcaaaacc tagaccactc
      601 aataaagtat tagcttcaac aatattttct aatgctggtg tcaccacttt agcttcacaa
      661 gctgccatag cctgtaaacc atctgcaaac agcgtttctt cacatttctt cgcaattgca
      721 actccagcca atgtttgctg ttgtcccaac atagtttttc catttgcctg cataaccgca
      781 cgcgcctcaa cccaagttgc taaaccatct gcaataccag acgctaataa acgcttaggg
      841 gcttgtgaaa taacttttgt atcaaccaaa actaaatctg gatttttaga ataaaataga
      901 taatgatcaa atgcaccttc atctgtataa ataacagata aagcagatac aggtgcgtcg
      961 gtcgatgcaa ttgttggagc aataataaca ggcttttcaa tcaaatctgc aatagctttt
     1021 gcgctatcaa tcgtctttcc cccaccaaga ccgataatac tatcacaatt ttctttctca
     1081 gccaaggcaa caactcgatt gatttcattg tcagaagctt caccattaaa tagcgccaga
     1141 acaatatgga aaccatacct atgtagataa tcttcaaatc gttttccaac aatatcataa
     1201 accaactgat cgcataatag aatagggcaa tttcccaaat ccaaaattga tttggcattt
     1261 tcaaacaagg cattttcccc ctgaatatat ctagaaggac tagcaaaaat tctcatatgt
     1321 atttcctctc tatctaaatg gaacgactat tttgaataac aaaccaatcg tcagaaaaat
     1381 catcaaccgc cttttggata gatggcatgg cgaaagctga ttcaaaaaca tccgctcctg
     1441 ctgtaacagc atgcgcacct gcagctaaag cattatttac ttgtgctaca tttttaaagg
     1501 atgcagctaa aatcttacta ggagagttct gtctatcaat agcaagagct aattgacgaa
     1561 tgacagaatt tgaatcaatg ttcagatttt ccattctatt ataatatgga gctaggtaat
     1621 ccgctcctgc ttcgatagct aataatccct gaataactgt ataaatagct gttgcagtga
     1681 tatggtagcc ctcttttttt agcgccttta ttgcacgtaa tccagctgga gtaacaggta
     1741 ctttgataaa tatatcatct cctgcttgtc ttcgaatttt atgagcatcc tttaagatgc
     1801 cttcaaaatc ttgagaaatc acctgaacat gaatagaggg tgtagagcca atcaattctc
     1861 ttacatcttt gattcgttca aaaaaattaa tagaaccctc tctttttgca atagtgggat
     1921 ttgaagttac cccagctagc ggcaaaattt cagaccactt tttaatctca tctaaattta
     1981 atgtgtcaag cataaattcc ataaaaagaa cctctttatt ttacaaagta tgctcagtac
     2041 gtcctataat gtcatcttgg gttgccttag aaagaacatt gaagaatgca gagtatcctg
     2101 caacacgaac aattaagtct ctgtgttttt caggatgttt ctgagcgtca atcagcgtct
     2161 ctctggaaac aacattgtat tgaatatggt acccatgtaa acgattaaag aatgttcgta
     2221 acaaagcaat tagttttaat ttatcttctt ctttggctaa cgtttgagga tttactttct
     2281 gatttaagag aaccccacct acgatttcat ctgttggtaa ttttgaaaca gattttaaaa
     2341 cagatgtagg gccgtgttga tccatattat gtgatggtga acaaccctct gctaacggtg
     2401 tacccgcgtt gcgtccatct ggagttgcta atgttccacg tccctgccct acgttggctg
     2461 agatagaaga tgttcctgaa taacgaattc ctccaattgg acctcttcca taacgtgtat
     2521 taggatattt agcaatttca tcaacataaa tgtcataagc agcagtaacc aatttgtcag
     2581 cataatcatc atcattacca tacttaggtg catcatgaat caacatttct tgaatgacct
     2641 taccttcttc tccggcataa tctgtttcca gtgcatgcca aagctgactt gggcttatac
     2701 gttcttcctc aaacaccaat tttttaattg cagctaatga atccgacaaa tttgcaattc
     2761 caacttgcaa tcctgatata taatcatata ctgctccacc ttctttaagg tgttttccac
     2821 gaccaataca atcatcagtc aatgctgaac atagaatatc aggaacttct cgttccaatg
     2881 ataaatcaat agaattttca acaataacac tcattcgtgt caaatatctt agtgttttat
     2941 cccaagcatt ttctaattca gaaaagttct tcatatcctt aaaatgacca aagcttggtg
     3001 caaaccgttt acccgaagcc ggatcaattc catcattcat cgtgataagt agaaccttag
     3061 ggaagttcat ataactcata cctgtgcaac gatagcccca tttccctgga actgccgttt
     3121 caacacatcc aatggcactg taatcataag catcatcttc caatactcct tttgcaataa
     3181 aagaaggaat aataatctca tcattattaa atgcaggcat accaaaacca agtttcatca
     3241 cttcaataca ctcattcatg aaacgagcat ctaaacctgc atggtaacgt acagttagat
     3301 taggttgcgg tagatgggtt tgtgcaactg attttaatac caaataagat aatgggttaa
     3361 cagcatcctt cttatctcga gtctgtccac caattgtaac attttgatat aaaggacttc
     3421 ctgctgaaga aaatgtatgt gattgactgc gaaccttatt aattgtaatt gtcttaatcc
     3481 aaagatttgt cagacgttca acaatgctat cttctgtttc tttaccactt tctaaatcag
     3541 ccttcatata tggatacata tattgatcaa aacggccata tgaaagagag tggccattag
     3601 attcaatttg taaaatacat tgaataaacc aaactgattg aatagcttct gcaaaagtag
     3661 ttaccggttc atatgggact ctagagcaaa tatctgcaat ctcaagtaat tctttcttac
     3721 gtttaggatt tgcattttcg gctaaacttt tagcaagagc aacaaagcgc tttgcatata
     3781 ctttaatagc atcgattacg ataaatatag agtcgtaaaa atgatattta tcaatacttg
     3841 ctggatctgt taaatctaga gctgcttttg ctttacgagc ccgctcttca aaacctctta
     3901 agccaaattg caaaagtttc tgatagttaa ctgctaggtg agcatctcca gaattcatct
     3961 taccttccat accgaagaat cctgtttcca tataaacaga cacttcttca ggtaataagg
     4021 caccagctct agcacgtaaa ttattatttt cccaaaacgg agcaatactt ctaagttgtt
     4081 cttttgtttc ttctgtaata tagaaaacat ctccatcacg cttttcaaaa agatccaact
     4141 cattgagaac aaattctagc gtatattccg gaaaaatagg agcatcttta ttggaagaag
     4201 cttgatttcc cccaatcata gattcttctt caatatagat agtcatattt tccaaaattt
     4261 ctttcagcat atatgcacgt tttaggacat taggttgttc cttgtatcgt tcataggcgc
     4321 gtgttgctag aactgctctc tcagcatcaa tataaggttt tttatttaaa acatcttctc
     4381 gatatttgtt catcctttca gtaaggctgc caaaatattc tgtcttgatt gttgttcttg
     4441 ctacttctgt atttaccatc gtaaccacct ttctttcgaa aatattttta tttctttcga
     4501 aaatataata ccgattttta tagaaatttt caagctttta actagaaaaa ttttaactct
     4561 ctaattttta gcacaaatgt gtgagtttaa cagtaacaca caaatacctc taagattgtt
     4621 tgatttcatt ttggtaagct aaacgatcct gtactttaaa gaatggaaaa taaaccaatg
     4681 tagacatcgc taatatcacc agctgagtaa taactccttg ccatccaccc accaaaaatc
     4741 ctgataaaat agctggtgta ctccaaggca atgttacccc tgagaatggc tgcatgaaac
     4801 ctgttgcaat agctccatat actatcacag ctgcaagtac aggaacaaga atgaaaggta
     4861 caaacataac tggattcatg acaatcggaa atccaaatac aactggctca tttacgttaa
     4921 atattgctgg aaaagcggca acttttccta aggcttggta ttgttttgat tttgctgcaa
     4981 aaagcatggc aactacaaga ccaaacgtaa tccctgaacc tgatagaatt aaaaatgaat
     5041 ctaaaaattg ttgagtaaca atatgtgcac cattttctaa tgatagatta ccagaggcta
     5101 acatagcttt attagcatca agattagata aaagcagagc tgtcactact ccatttacta
     5161 ccgattgtcc atgaacacca aaccaccaca aaaatgatat aaagaatgca attccaatag
     5221 caccatacaa agatccagtt aaaccttgca atggaacttg aatagcagaa taaatcattt
     5281 ctatgaatgt tccgccatta gtcaatgact tcgctaaaat atatacaatc atagaaagta
     5341 agaaaattac aaatgctgga atcattgctt caaactgttt ggcaatagct tgtggaactt
     5401 gttctggcat cttaataaca atttttctct ttataaagaa ggtataaata cttcctacta
     5461 ccaaacctat aatgatagca ccgataattc cttgacctcc aaaccaaact ttactaatag
     5521 catccccaat tgcctcacct tgtttaggga tataagatga tcttagcaaa ataaagaatg
     5581 cagatacaga tagaactcca gctggtaaag cctctactcc gctattctta gcataagaat
     5641 aggcaattga aaaacaagaa attagaccca taatagcaaa agttcctgaa tatacttgca
     5701 taaacggctc tgtccaatta gctccaaaaa cactagcaat gctcttattt aatccttcga
     5761 acggcaattg tcccataatc aagaataaac taccaactac tgtcaatggc aaaattgcta
     5821 acatcccatc ttttagagct ataatgccac gcatattcac aaacttcatc atcggtgcaa
     5881 tgattttctg aacatccatc tttgccataa taaatctcct tttcttaccc actaatcaaa
     5941 gatagggcca aatctaatac ttttttccca tctaacatac catagtccat catcggaata
     6001 acagctatcg gaacatcaca cttatcacaa atttcttttg atttatctaa tgtataagca
     6061 acttgtggac ccaatagtgc aacatctata tttggcgcat aatccgctaa tttagactga
     6121 gaaaacgcct ctatttctgc ctcaactcca ctagcttgcg ctgcaatttt catattattt
     6181 acaagcatac cagtagaaaa acctgctgca caaaacaaac caatcttcac cattatgttt
     6241 tcctcctcta tgttaataac aatgataata ctctagtatt attttttatg aagttctttt
     6301 ctcaaactaa taatttcttt gattaaatta atctccgtca tactagtcat gagatgatct
     6361 tgtgaatgaa caaagagagc tgtaatttct gtctttgtac cagacgcttc ctgtgccaag
     6421 aattttgttt gtagattatg agcttctaat aaggctgcat cagctaactg gatttcttct
     6481 tcacatcgtt cacatgtacc attttttatg taatctaacg ctttataaat atgttgtttc
     6541 gcatcgccag catataaaat taaacccatg ataatttgat ctggaacaat aatttccata
     6601 aattcctcct catttcacta tgtaaaaagc ttttttcatt tgaaatttta
//
LOCUS       DAVLXF010000097         6595 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLXF010000097 DAVLXF010000000
VERSION     DAVLXF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.097"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(8..763)
                     /locus_tag="VYO12_001841"
                     /pseudo
     CDS             complement(8..763)
                     /locus_tag="VYO12_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(901..2523)
                     /gene="groL"
                     /locus_tag="VYO12_001842"
     CDS             complement(901..2523)
                     /gene="groL"
                     /locus_tag="VYO12_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1588387.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2539..2823)
                     /gene="groES"
                     /locus_tag="VYO12_001843"
     CDS             complement(2539..2823)
                     /gene="groES"
                     /locus_tag="VYO12_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1588388.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3093..3470)
                     /locus_tag="VYO12_001844"
     CDS             complement(3093..3470)
                     /locus_tag="VYO12_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1588389.1"
                     /translation="MIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREADFVN
                     MVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLLESRAQR
                     AMRENNAGQDLADLVLEEEELPF"
     gene            complement(3497..3739)
                     /locus_tag="VYO12_001845"
     CDS             complement(3497..3739)
                     /locus_tag="VYO12_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588390.1"
                     /translation="MKILNIKLANVEQTDSGFEHWVDVTYNVPILKNEYTVRLLLLFG
                     FEIEDPEVIEYLVNAWKYRELVLHSVRMYDMEREAS"
     gene            complement(3971..4927)
                     /gene="lytA"
                     /locus_tag="VYO12_001846"
     CDS             complement(3971..4927)
                     /gene="lytA"
                     /locus_tag="VYO12_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1588391.1"
                     /translation="MDIDTSRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKVEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLAD
                     RWKKYTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPDGLITVK"
     gene            complement(4933..5262)
                     /locus_tag="VYO12_001847"
     CDS             complement(4933..5262)
                     /locus_tag="VYO12_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1588392.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKNYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKG"
     gene            complement(5266..5565)
                     /locus_tag="VYO12_001848"
     CDS             complement(5266..5565)
                     /locus_tag="VYO12_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588393.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
     gene            complement(5574..5924)
                     /locus_tag="VYO12_001849"
     CDS             complement(5574..5924)
                     /locus_tag="VYO12_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588394.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            complement(5927..6130)
                     /locus_tag="VYO12_001850"
     CDS             complement(5927..6130)
                     /locus_tag="VYO12_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588395.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(6111..6227)
                     /locus_tag="VYO12_001851"
                     /pseudo
     CDS             complement(6111..6227)
                     /locus_tag="VYO12_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
     gene            complement(6224..>6595)
                     /locus_tag="VYO12_001852"
     CDS             complement(6224..>6595)
                     /locus_tag="VYO12_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV1588396.1"
                     /translation="WNPKGGRNAVVWWNQVGSGSVKYWMEQKSDRRLKENITDTAVKA
                     LDKINRLRMVAFDFIENKKHEEIGLIAQEAETIVPKIVSRDPENPDGYLHIDYTALVP
                     YLIKAIQELNQKIEKMEKTIA"
BASE COUNT         1685 a         1508 c         1252 g         2150 t
ORIGIN      
        1 tctagtttca atctactata aatttgatat gagaatagtc tttctcaaca tccttaccca
       61 acaagaatgc tgtagcgtcc ttcttaactt gaaccaggtc aatattctgg gcttgggctg
      121 catagacgca gccacaataa cattgacgat agatatcata ctcctcacac atctctactg
      181 aacgtttgta gccttgattt ttcttgaaat cgttgggaag atagtgggtc gtgtaaattt
      241 tttgcacatc gattccgatg ctgttgatgg tttgagaatt cttatgagga ctgatggtca
      301 aggctgaacc aaagtagtca aagcccaagt ccatagccac ttgcgctgtt ttatccagtc
      361 ggtagtcaaa acaaaccttg caacggtcgc caccttcggg ctcctcctct agtcccctaa
      421 ctagttttcg gtattaattg ggttcgtagg gagcttctag gtactgaacc gtatttcctg
      481 tccgctcatt aaaatcacta acaaatttct tgatgacata gacccgctta tggtattcta
      541 ccttgggatg gatattagaa ttggcaaaat agatggtcac atctgcatac ttggtcaaat
      601 attctagtgt ataggtacta caaggggcac agcaaacatg cacgagaatg gttggccgct
      661 gctcattttt ctcccatgct tgtaccattt tctgcatgac acggtcataa ttaatcttct
      721 gattgggggg catcttgcac agaatttctt ctacatcgat catgttcttc tcctttttct
      781 agtcttattt tatcatataa gttaggagag ctcaaatcaa tttagaagtg aatattataa
      841 aaagaagggt tagtcattcc ctccttttgt gttttttata agttgttttc tatagaaagc
      901 ttacatcatc ccgcccatca tgcttggatc cattgctgga gctggggcta ctggttctgg
      961 tttattggct acgactgctt ctgttgtcaa aatcaagctg gctacagatg ctgcattttg
     1021 tagggctgaa cgactcactt taactggatc aatgatacct tgatcaatca tgttaaccca
     1081 ctcgccagtt gctgcgttga atcctatacc aagctcagca tttttcaaac gatcgataac
     1141 gatagatcct tcaaatcctg cattgtgagc aatttgacga acaggttctt ccaaagcacg
     1201 gagaacaata ttacgtcctg ttgcttcatc tcctgtcaat tccaaggtag caacagctgg
     1261 aatcacattg gcaagagctg ttccaccacc tgcaacaata ccttcttcaa cagctgcacg
     1321 agtagcgttg agggcatctt caatgcggag tttcatttct ttcaactcag tttcagttgc
     1381 ggctccgacc ttaataaccg ctacaccacc tgacaatttg gccaagcgtt cttgcaattt
     1441 ttcacggtca aattcagaag ttgtagtttc gatttgagac ttgataaccg caacacggtg
     1501 agaaatcgct tcaggatttc ctgcaccttc tacaataacc gtgctatctt tgtccacggt
     1561 cactctcgct gcttgaccaa gagcttcaat tgtcgcatct ttcaactcaa gaccaaggtc
     1621 ttctgtgata actgttccgc ctgttaagat ggcgatatct tcaagcatag ctttgcgacg
     1681 gtcaccaaaa ccaggtgcct tgactgctac tacgttgaag gttccacgaa tcttgttcaa
     1741 aacaagagtt ggaagagcct cgccatccac atcatccgca ataatcaaga gtggacgatt
     1801 gctttggaga atgctttcca aaagtggcaa gatttcttgg atattggaaa ttttcttgtc
     1861 tgtaatcaaa atgtacggat tttcaaggtc agccaccatt ttttcgctat ctgtcaccat
     1921 gtactgtgaa aggtaaccac ggtcaaactg cattccttct acgacttcaa gctctgtttc
     1981 cataccacgt gactcttcga tggtgatgac accgtctttg ccaacttttt ccattgcttc
     2041 agagatgtac tcaccaactt tttcagaacg agaagatacg gctgcaactt gagcgatagc
     2101 ttctttattg gcaacaggga tggcgttgtt tttcaaagct tcaactgctg cggcaactgc
     2161 tgtttcaatc ccacgacgaa taccgattgg atttgcacct gctgtgacgt ttttgattcc
     2221 ttcacggacg atagcttggg tcaagactgt tgcagtcgta gttccgtcac ctgcgatatc
     2281 attggtttta gaagctactt ctgatactaa cttagcaccc atattttcaa aatggtcttc
     2341 caattcgatt tctttggcaa tggtcacacc gtcattggta atcaagggtg aaccgaatga
     2401 cttttcaaga acgacattgc gaccttttgg tcccaaggtt actttaacag tgtctgcaag
     2461 gatatcgaca ccacgaacca tggctgaacg ggcatctgat gaaaatttaa tttcttttga
     2521 catacttact ttctccttct attcctcaat gattgccaag atgttagctt cgcctacgat
     2581 gatgtacttt tcatcgccat ctttgacatc aagacctgcg tgggcttcaa ctaagacacg
     2641 atctccagtt ttaacacttg gagcaaccaa gtcaccgttc aaggtacgaa caccttgtcc
     2701 agtagccaca acttgagctg ttttggtttt ttcttgggct gagcctgcaa ggacaaagcc
     2761 cccaacggtt tgttcttttt cttctatttt taagaccaca cggtccccta atggtttcaa
     2821 catctgattt cctccataat gagatagata ttattagcac tctttgaact tgagtgctaa
     2881 ttcataattc tattgtatca cttggtcaga aatagtcaag aaaaaagtct gactttctca
     2941 agataaaaag cctgagacca actcagactt tttaatgttt atactcaatg aaaatcaaag
     3001 agcaaactag gaagctagcc gcaggctgct caaagcattg ctttgatgtt gtggataaag
     3061 ctaacgtggt ttgaagagat tttcgaagag tattaaaatg gcaattcttc ctcttccaag
     3121 accaaatctg ccaaatcttg gcctgcatta ttttcacgca tggcacgttg agcacgactt
     3181 tctaagagtt ggaatcctgt cacaaggact tcggttacat agttcatctg accatttttc
     3241 tcaaagcgac gggtacgcaa ttctccatca acggaaatga gactaccttt ggttgcgtag
     3301 cttgccaaag tttctgctag cctgccccat aggaccatat tgacaaaatc agcttcacgt
     3361 tcaccgtttt ggtctttgta acgacggttg acagcgatag ttgctcgcgc taccgacttg
     3421 tcattgttgg ttttgtgcaa ttctggtgta gacgttaaac gcccaatcaa gataagttag
     3481 agttagtaac agtattttaa cttgcttctc tctccatatc atacatcctc acagaatgta
     3541 agaccagctc acgatatttc caagcgttga ccaggtattc aatcacttca ggatcttcaa
     3601 tttcaaaacc aaagagcagc aacaatctaa ccgtgtattc gtttttcaaa attgggacgt
     3661 tgtaagtcac atccacccaa tgctcaaagc ctgaatctgt ctgctctaca tttgctagtt
     3721 taatatttaa aatcttcatt tttattcctc ctacttatct attcgtaaaa gaagataaaa
     3781 agttatgaaa aaatcattac ttttttaatt cagacagtac tttcagagca attttgttaa
     3841 tgtcaacaaa attggcaacc aagcgattta atactatttg ttgacgccaa caagtcaatt
     3901 ttcagacaaa caaaaaaacc gcaagcctga gcctgcggtg aaagaacaat ttagaaagtt
     3961 tcctttctat ttatttaact gtaatcaagc catctggctc tactgtgaag tctggcttat
     4021 ctgccagtgt tccgtctggt ttgaggtagt accagcctgt tccgtctgcg gactggataa
     4081 aggcatttga taccatagcg ccttctttag cgtctaagta gtaccaagtg tccttgtact
     4141 tgacccagcc tgtcttcatg gcaccttctt cgttgaaata gtaccacttc tcagcaattt
     4201 tcttccagcc tgtagccatt tcgcctgagt tgtcaaacca gtaccagttg ccgtctgtgt
     4261 acttcttcca gcggtctgca agcatatagc ctgagccatc gaaataatac caggtaccgt
     4321 tgattttctc aaacttatct tttggataag agccgtctga atgtacgtac cagtagccag
     4381 tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt ctcaatatca tgcttaaact
     4441 gctcacggct aatgccccaa cttgcaagat atggatatgg atccacatgg tctgagtggt
     4501 tgtttggttg gttattcgtg caatactcgt gcgttttaat tccagctaaa ctccctgtat
     4561 caagcgtttt cggcaaacct gcttcatctg ctagattgcg taagagttcg atataaaggc
     4621 ggtagtccgt catgaactct actttagttg aatggctttc aatcagttca accgctgcgt
     4681 aactctcagt attccaaccg cccccaacat cccaacttcc gttgttcaca ggacctactt
     4741 gcatgacacg gccgtttccg acaacatgtg aaaagaaccc tagttcaggg tcctttctat
     4801 agtggtaatc agcctcattt tgagctgttg agttacggtt gcctgttgag tgggcgtgta
     4861 cttgtcgata aggctgcaca ccgacctgag gcaagcctgt acgtagtctg cttgtatcga
     4921 tatccattac tcttatcctt tccaagcgtc gttcatctgt ttcactgcgg actctacgaa
     4981 ggtgtctaag tctttgtcag tcatgctaat attgtatttt gtaagctcag cacggacttt
     5041 agcgcgagcc tgtgccagtt tttcatctcc cttgtagcca gtttcagcag ctacttgctc
     5101 aacggcatta actgcgttct tagccaagat ttcaacaatc ttgatagtct tttctccacc
     5161 tttttgaacc aggtagttct tgactgcctt gactgcgata ccagccaaaa taacaaggat
     5221 gctgattgct ccattagtaa tgatttcagt aatttgttgc atttgttatt ctcctttttc
     5281 gatttcttcc atgcggtcgt tcatgcgaac catttctttt tgaatgtctc cgaccgtgtg
     5341 agtgattgtg gttaattctg tagtggtctt ttctaggtga gtcatcaaac gctcttctcg
     5401 tctattagag tcggcctttg attgctcgtg caaatccata atcttcttct ctcgcttgtc
     5461 cgaagtcttg ataagatatc gaatgataat aaaaaaaagc aagataaaca aaatcgccca
     5521 agctacctga ctttgagcga ttttttcagc ttcttcaatt ggcatacaac ctcctattct
     5581 ttaggttcta ccgttggaac cgtccagtca ggattgccct ctgcatcaaa tttcatgata
     5641 tagaattcat gattcaacag aacggctacg ttgattgttg cgattgtacc accccactga
     5701 ttgaacgccc aaacggtttc aacatccttg aattggcgac ggccatttac aatcacagga
     5761 cgtttttgaa cgtcacgata catatagaag tcatcgctta cattcttgca acgaatgaac
     5821 tctccatttt ctttcatgta gcgcaaagcg ctcgcaagat caaatggttc tgtgattttt
     5881 gtaaggtcta gtaagttatc tgtgttttga attgtttctg ccatgtctat tctcctttgt
     5941 ctgctggttt agtttgttca tcaagcagag cttccagctc atccactcgt gcttgaagtc
     6001 tttgattctc ttccctttgc tcattcaact gaatactcaa gatattactt gtaatcatcg
     6061 aatctgttga agttgttgac atttcacgaa ttgtcatttg taaggcttgg ttaagctgtt
     6121 ctgtgttcat tttctaagtt ctccaatctg tgtgttcgtt ttctattttc aagagcaagc
     6181 tcctaaattg ctttaagtgc gatattggtc aacatgttgt tattcatgct attgttttct
     6241 ccattttttc tattttttga tttaattctt gaatagcctt gattaagtaa ggaactaaag
     6301 cggtatagtc gatatgcaga tagccatctg gattctcagg atctcgtgag acaatttttg
     6361 gaacgatggt ttcagcctct tgagctatta gaccaatctc ctcatgtttt ttattttcga
     6421 tgaaatcaaa tgcaaccatt cttaatctgt tgattttgtc caaggctttc acagctgtat
     6481 ctgtgatgtt ctcttttaag cgtctgtctg atttttgttc catccaatac ttcacgctac
     6541 cgctaccgac ctgattccac caaacaaccg cattccttcc gcctttggga ttcca
//
LOCUS       DAVLXF010000098         6162 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLXF010000098 DAVLXF010000000
VERSION     DAVLXF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.098"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..437
                     /locus_tag="VYO12_001853"
     CDS             <1..437
                     /locus_tag="VYO12_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1588397.1"
                     /translation="LEADNQEQLFDQVASLLEEREIVTPTYREALITREKSFPTGLDM
                     EFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNS
                     SSSQTNILAQLMNFFTGNGHLEDLSKISEPEKLYAYIDEAIA"
     gene            475..777
                     /locus_tag="VYO12_001854"
     CDS             475..777
                     /locus_tag="VYO12_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1588398.1"
                     /translation="MIKILAACGAGVNSSHQIKSTLEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSAPVFDNIRLPAKQNMV"
     gene            785..1588
                     /locus_tag="VYO12_001855"
                     /pseudo
     CDS             785..1588
                     /locus_tag="VYO12_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1637..2101
                     /locus_tag="VYO12_001856"
     CDS             1637..2101
                     /locus_tag="VYO12_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1588399.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTSLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2258..2683
                     /gene="lacA"
                     /locus_tag="VYO12_001857"
     CDS             2258..2683
                     /gene="lacA"
                     /locus_tag="VYO12_001857"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1588400.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2714..3229
                     /gene="lacB"
                     /locus_tag="VYO12_001858"
     CDS             2714..3229
                     /gene="lacB"
                     /locus_tag="VYO12_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1588401.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3240..4169
                     /locus_tag="VYO12_001859"
     CDS             3240..4169
                     /locus_tag="VYO12_001859"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1588402.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            4171..5151
                     /gene="lacD"
                     /locus_tag="VYO12_001860"
     CDS             4171..5151
                     /gene="lacD"
                     /locus_tag="VYO12_001860"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1588403.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQRTATSWKERV"
     gene            5557..6033
                     /locus_tag="VYO12_001861"
     CDS             5557..6033
                     /locus_tag="VYO12_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588404.1"
                     /translation="MNVDGNKFYNVSVSEDIVNAGQILEDYFYVDKFGNINLKGTPEE
                     LAKNIGISVQEASLMYEAVKELPNVYERGPVGFRFNLGPQVRGMGGWAAGAFATGYAG
                     WHLKQFAVNPVTSGFVAVISGAIGWAVKTAVENYWTVAVATVEVPFVNLVYTIDLP"
BASE COUNT         2012 a         1050 c         1288 g         1812 t
ORIGIN      
        1 gcttagaagc ggataatcaa gaacaactct ttgatcaggt agcaagcttg ttggaagaac
       61 gagaaattgt cactcctact tatcgtgaag ccttgatcac gcgtgaaaag tcatttccaa
      121 ctggtttaga tatggaattt ctaggaaagg acttgccaaa tgtagcgatt cctcacacag
      181 atattgttca taatctagct gagaaagtgg tggttgttcg attagaaaaa ccagtaactt
      241 ttcacaatat gatagctccc gataaggaag tagaagtatc cctactcttc tttatcatta
      301 ataattcaag ttcaagtcaa acgaacattc ttgctcagtt gatgaacttc ttcacaggaa
      361 atggacatct tgaagaccta tcaaaaattt ccgaaccaga aaaactttat gcttatatag
      421 atgaagcaat cgcttaatct tgtctattaa aaaataaaat cggaggaagt ctatatgatt
      481 aaaattcttg ctgcctgcgg tgcaggtgtt aactcaagtc accaaattaa aagtactcta
      541 gaagaagaac tttcaaaccg tggttacgat gttcactgtg atgcagtcat ggtgaaagat
      601 gtaaacgaag accttatgaa aggttacgat atctttacac caatcgctgc aacagatctt
      661 ggttttgaac caggtatccc agttatcgaa gctgggccaa tcttattttg tatcccagca
      721 atgagcgctc cagtatttga caatattaga ctccctgcga aacaaaatat ggtatagtag
      781 ttctatgaat gatgaagcaa gtaaacaact aaccgatgca cgatttaagc gtcttgttgg
      841 tgttcagcgt accacttttg aagagatgtt agctgtatta aaaacagctt atcaacttaa
      901 acacgcaaaa ggtggacgaa aacctaaatt aagcctagaa gaccttctta tggccactct
      961 tcaatatgtg cgagaatatc gcacttatga acaattgcgg ctgattttgg tatccacgaa
     1021 agcaacttac tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag tggttttacg
     1081 atttcaagaa ctcctctcag ttctgaggac acggtaatga ttgatgcgac gaaagtaaaa
     1141 atcaatcgcc ctaaaaaaag aattagcgaa tgattctggt aaaaagaaat gccacgctat
     1201 gaagtctcag gcgattgtca caagtcaagg gagaattgtt tctttggata tcactgtgaa
     1261 ctattgtcat gatatgaagt tgttcaaaat gagtcgcaga aatatcggac aagctggaaa
     1321 aatcttggct gacagtggtt atcaagggcc catgaagata tatcctcaag cacaaactcc
     1381 acgtaaatcc agcaaactta agccgctaat agctgaagat aaagcctata actatgcgct
     1441 atctaaggag agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatgtt
     1501 ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg
     1561 tattatcaat catgaactag gattctagtt tttcaggaag tctaataaag aaactagcaa
     1621 ggtcaggcct tactttattg gtggacatcc aggatttaat tgtcctctac ttgatgatga
     1681 agtctatgaa gattattatc tagagtttga gaaagaagag acttgctctg ttccacgtcc
     1741 tttcccagaa acaggtatgt tggatttcca agatagaagt ccatggctag agggacaaaa
     1801 agaaatagat cttagttatg atcttttcag cacagatgca gtgactttgg atgaattgca
     1861 atctcgaaca attgcccttc gttctcttaa acatgataag ggattgaaag tgcattttgc
     1921 agaatttcca aacctcatca tctggtcaac tttgaacaag ggacctttca ttacctttga
     1981 accatggtct ggcttgtcaa catcccttga agaaggagat catttagaag ataagaagaa
     2041 tgtttgtctc ttagaagcca atcaggttga agaattaggg tttgagatag aagttttata
     2101 aaaaagtaaa aaataaataa caaaaacaaa cacaaactat tgacttcttc aaacaatagt
     2161 agtatattat gtttgtgagg actaattgac tgttttttaa agatagccaa tacactgctg
     2221 gaatccagca tagaaaaaat aaaaaggagt atacaatatg tctattgtta tcggtgcaga
     2281 tgctgcaggt ttgagattga aagaagttgt gaaagacttc ttagaaaaag aaaacttcca
     2341 ccttgtggat gttacagctg aaggtcaaga ctttgttgat gtgactctcg ctgttgctgc
     2401 agaagtaaac aaagaagaac aaaaccttgg tatcgtgatt gatgcttatg gagctggtcc
     2461 atttatggtt gcaactaaga tcaaaggaat ggttgctgca gaagtatctg acgaacgttc
     2521 agcttatatg actcgtggcc acaacaactc acgtatgatc actatggggg cacaacttgt
     2581 tggtgatgaa ttggctaaaa acatcgctaa aggatttgtt aatggtaaat acgacggtgg
     2641 tcgtcaccaa atccgggttg acatgttgaa caaaatgggc taattagatt atagtaagaa
     2701 aggtaagtta aaaatgagaa ttgcaattgg atgtgaccac atcgtaactg atgaaaaaat
     2761 ggcggtttca gaatttttga aatcaaaagg atatgaagtc attgactttg gtacatatga
     2821 ccatacacgg actcactacc caatctttgg taaaaaagta ggggaagcag taactagtgg
     2881 tcaagctgat ctcggggtat gtatctgtgg tactggtgtt ggtatcaaca acgctgtaaa
     2941 taaagttcca ggtgttcgtt ctgccttggt tcgtgatatg acaacagccc tttatgctaa
     3001 agaacaattg aacgctaacg ttatcggctt tggtggtaag attactggtg aattgcttat
     3061 gtgtgatatc atcgaagctt tcattcatgc tgaatacaaa ccatctgaag aaaacaagaa
     3121 attgattgcg aaaattgaac accttgaaag tcacaatgct caacaaacag acgcaaactt
     3181 ctttacagaa ttccttgaaa aatgggatcg cggagaatac cacgactaag aggtgaccta
     3241 tgattttaac agtcacaatg aacccatcca ttgatatttc ctatcccttg gatgagttga
     3301 agattgatac tgtcaatcgt gtggtggatg taaccaaaac ggctggtggt aagggactta
     3361 atgttacccg agtactttca gaatttggcg attctgttct tgctactggt ttagtgggtg
     3421 gcaaacttgg tgagtttttg gttgaacata tcgataatca agtaaagaaa gatttcttct
     3481 caattcaggg agaaactcgt aactgtatcg ctattctcca cggagacaac caaacagaag
     3541 ttcttgaaaa aggtcctgaa gtattggaac aggaaggtca agactttttg gaacatttca
     3601 aaaaactctt ggagtcagtt gaagtagtgg ccatctcagg tagtctgcca gctggccttc
     3661 cagttgatta ctatgcgagc ttggtagaac ttgctaatca agctggcaag cctgtagtct
     3721 tggactgctc aggtgcagca cttcaggctg ttcttgaatc accccataaa ccaacagtca
     3781 tcaaaccaaa taatgaagaa ttgtctcagc ttcttggaag agaagtttct gaggatttgg
     3841 atgaattaaa agaagtactt caagaacctt tgtttgcagg gattgaatgg attatcgttt
     3901 cacttggtgc caacggtact tttgccaaac atggtgacac tttctacaag gtagatattc
     3961 ctagaattca ggtggtaaat cctgttggat ctggagactc tactgtggca ggaatttctt
     4021 caggacttct tcacaaagaa tcggatgcag aattactcat caaggcaaat gtccttggta
     4081 tgctcaatgc tcaagaaaaa atgactggtc atgtcaacat ggccaactat caagctctat
     4141 atgatcaatt aatagtaaaa gaggtataaa atggctttaa cagaacaaaa acgtgtacgc
     4201 ttagaaaaac tttctgatga aaatggtatc atctcagctc ttgcatttga ccaacgtggt
     4261 gctttgaaac gcctcatggc tcaacaccaa acagaagaac caactgtggc ccaaatggaa
     4321 gaacttaaag tcttggtagc agatgaattg actaaatacg cttcatctat gcttcttgac
     4381 cctgagtatg gacttccagc aactaaagct cttgatgaaa aagctggtct tctccttgct
     4441 tatgaaaaaa caggttatga cacaacaagc acaaaacgct tgccagactg cttggatgtt
     4501 tggtctgcaa aacgtattaa agaagaaggt gcagatgcag ttaaattctt gctttactat
     4561 gatgtagata gctcagacga actcaatcaa gaaaaacaag cctacatcga acgcatcggt
     4621 tctgagtgtg tggctgaaga tatcccattc ttccttgaaa tccttgctta cgatgaaaaa
     4681 attgcggatg caggttctgt agaatacgct aaagtaaaac cacacaaagt tatcggcgct
     4741 atgaaagtct tttcagaccc acgctttaac attgatgttt tgaaagttga agttcctgtt
     4801 aacattaaat atgttgaagg cttcgctgaa ggtgaagtag tttatacacg tgaagaagca
     4861 gcagccttct tcaaagcgca agatgaagca acgaacttgc catacatcta cttgagtgct
     4921 ggtgtatcag ctaaactctt ccaagatact cttgtatttg ctcatgaatc aggtgcaaac
     4981 tttaacggag ttctttgtgg ccgtgctaca tgggcaggat cagttgaagc ttacatcaaa
     5041 gatggtgaag cagcagctcg cgaatggctt cgcacaactg gatttgaaaa cattgacgaa
     5101 ctcaacaaag ttcttcaaag aacagcaact tcatggaaag aacgcgtgta agaaagtcct
     5161 cctagtttag gaacatgaat ctaaaaaaat ttaaaaaaag ttgtatgtaa aggtttacaa
     5221 aataacttac ttgtgctata cttaaatcac aagttaatat gaattagaaa gtaactatat
     5281 gaagtataat aaaaatagga tatagtttat tttacgagct aggaaggaaa aatacggaaa
     5341 caatattgcc agaataaact atatttagat gcacatttca ttcattgttt tataaaagga
     5401 gaagataaac ggctactaaa aagagtttta aagcgttagt tgtaggacta ggtattgttt
     5461 caatattctc atcagcctta cctatggtta gtggttctgt atttgcagat agtgccctaa
     5521 ctacagtata cagtagataa agcaaatgat attgttttga atgttgatgg gaataaattt
     5581 tataatgttt cggtttcaga agatattgta aatgctggtc aaattttgga agattatttt
     5641 tatgtagata aatttggaaa tataaattta aaaggcactc ctgaagagtt agcaaaaaat
     5701 attggtattt ctgtacaaga agcaagtttg atgtatgaag ctgtaaaaga gttacccaac
     5761 gtttacgaaa gaggtcctgt aggttttcgt ttcaatcttg gtcctcaagt gagggggatg
     5821 ggtggctggg ctgctggagc tttcgctact ggatatgctg gatggcattt gaaacaattt
     5881 gcggttaatc ctgttacatc tggatttgtt gctgtaataa gtggtgcgat tggctgggct
     5941 gtaaaaactg ctgtagaaaa ttattggaca gttgctgtag ctacagtaga agtgccgttt
     6001 gtgaaccttg tttacaccat agatttacct tagaggttat ttctttatga atcattcttt
     6061 taaaaaaata actgtatttt gttttatagt ttcttgtgtt ctttgtttat tagacttaat
     6121 gaattttaaa aatgtagcta cttttttatt tttctgtctt cc
//
LOCUS       DAVLXF010000099         6058 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLXF010000099 DAVLXF010000000
VERSION     DAVLXF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.099"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            301..>6058
                     /locus_tag="VYO12_001862"
     CDS             301..>6058
                     /locus_tag="VYO12_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1588405.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKE
                     ENSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKGLSNLKNTKVLYT
                     YDRIFNGSAIETTPDNLDKIKQIEGISSIERAQKVQPMMNHARKEIGVEEAIDYLKSI
                     NAPFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDK
                     IPHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNA
                     QIFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRA
                     LRKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQ
                     TVEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLD
                     LRGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEG
                     TKSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNV
                     GDEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVI
                     NGKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIAL
                     QNTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYG
                     SISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDG
                     KQIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFES
                     VEEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDG
                     KPKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSS
                     GSKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVI
                     SREHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPAT
                     KIRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLI
                     TKDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTY
                     AGEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFADQADEK
                     GMISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEG
                     EASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKRNIIA
                     YDDGSALQYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKE"
BASE COUNT         2382 a          833 c         1166 g         1677 t
ORIGIN      
        1 atctacaacc tcaaaacagt gttttgagca acctgcggct agtttcctag tttgctcttt
       61 gattttcatt gagtactaat cttatttttt aacgtgtgct aaaaaataaa ttaatgcatg
      121 ttaatttttt tgacttaaat ttttttaaat gatatactgt ttttatagag agataacaat
      181 tatatataaa tgtactactc tatttcctag ataatactta gtaaaacttt ttataacaaa
      241 ggctagcaaa gttaaagttt attatctatt ggaagttaaa taaatatgta aggaattaaa
      301 atgaaaaaaa gcacagtatt gtcattaact acagctgcag ttattttagc agcctatgcc
      361 cctaatgagg tagtcttagc agacacatct agctctgaag atgctttaag catctctgat
      421 aaagaaaaag tagtagtaga taaggaaaca gaaaataaag agaaacataa agatattcat
      481 aatgctatag aaacttcaaa ggatactgaa gaaaagaaaa caacaattat tgaggaaaaa
      541 gaagttgtta gtaaaaatcc tgtaatagac actaaaacta gcaatgaaga agcaaaaatc
      601 aaagaagaaa attccaatca atcccaagga gatcatacgg actcatttgt gaataaaaac
      661 acagaaaatc ccaaaaaaga agataaagtt gtctatattg ctgaatttaa agataaagaa
      721 tctggagaaa aagcaatcaa gggactatca aatcttaaga atacaaaagt tttatatact
      781 tatgatagaa tttttaacgg tagtgccata gaaacaactc cagataactt ggacaaaatt
      841 aaacaaatag aaggtatttc atcgattgaa agggcacaaa aagtccaacc catgatgaat
      901 catgccagaa aggaaattgg agttgaggaa gctattgatt acctaaagtc tatcaatgct
      961 ccgtttggga aaaattttga tggtagaggt atggtcattt caaatatcga tactggaaca
     1021 gattatagac ataaggctat gagaatcgat gatgatgcca aagcctcaat gagatttaaa
     1081 aaagaagact taaaaggaac tgataaaaat tattggttga gtgataaaat ccctcatgcg
     1141 ttcaattatt ataatggtgg caaaatcact gtagaaaaat atgatgatgg aagggattat
     1201 tttgacccac atgggatgca tattgcaggg attcttgctg gaaatgatac tgaacaagat
     1261 atcaaaaact ttaacggcat agatggaatt gcacctaatg cacaaatttt ctcttacaaa
     1321 atgtattctg acgcaggatc tgggtttgcg ggtgatgaaa caatgtttca tgctattgaa
     1381 gattctatca aacacaacgt tgatgttgtt tcggtatcat ctggttttac aggaacaggt
     1441 cttgtaggtg agaaatattg gcaagctatt cgggcattaa gaaaagcagg cattccaatg
     1501 gttgtcgcta cgggtaacta tgcgacttct gcttcaagtt cttcatggga tttagtagca
     1561 aataatcatc tgaaaatgac cgacactgga aatgtaacac gaactgcagc acatgaggat
     1621 gcgatagcgg tcgcttctgc taaaaatcaa acagttgagt ttgataaagt taacataggt
     1681 ggagaaagtt ttaaatacag aaatataggg gcctttttcg ataagaataa aatcacaaca
     1741 aatgaagatg gaacaaaagc tcctagtaaa ttaaaatttg tatatatagg caaggggcaa
     1801 gaccaagatt tgataggttt ggatcttagg ggcaaaattg cagtaatgga tagaatttat
     1861 acaaaggatt taaaaaatgc ttttaaaaaa gctatggata agggtgcacg cgccattatg
     1921 gttgtaaata ctgtaaatta ctacaataga gataattgga cagagcttcc agctatggga
     1981 tatgaagcgg atgaaggtac taaaagtcaa gtgttttcaa tttcaggaga tgatggtgta
     2041 aagctatgga acatgattaa tcctgataaa aaaactgaag tcaaaagaaa taataaagaa
     2101 gattttaaag ataaattgga gcaatactat ccaattgata tggaaagttt taattccaac
     2161 aaaccgaatg taggtgacga aaaagagatt gactttaagt ttgcacctga cacagacaaa
     2221 gaactctata aagaagatat catcgttcca gcaggatcca catcttgggg gccaagaata
     2281 gatttacttt taaaacccga tgtttcagca cctggtaaaa atattaaatc tacgcttaat
     2341 gttattaatg gcaaatcaac ttatggctat atgtcaggaa ctagtatggc gactccaatc
     2401 gtggcagctt ctactgtttt gattagaccg aaattaaagg aaatgcttga aagacctgta
     2461 ttgaaaaatc ttaagggaga tgacaaaata gatcttacaa gtcttacaaa aattgcccta
     2521 caaaatactg ctcgacctat gatggatgca acttcttgga aagaaaaaag tcaatacttt
     2581 gcatcaccta gacaacaggg agcaggccta attaatgtgg ccaatgcttt gagaaatgaa
     2641 gttgtagcaa ctttcaaaaa cactgattct aaaggtttgg taaactcata tggttccatt
     2701 tctcttaaag aaataaaagg tgataaaaaa tactttacaa tcaagcttca caatacatca
     2761 aacagacctt tgacttttaa agtttcagca tcagcgataa ctacagattc tctaactgac
     2821 agattaaaac ttgatgaaac atataaagat gaaaaatctc cagatggtaa gcaaattgtt
     2881 ccagaaattc acccagaaaa agtcaaagga gcaaatatca catttgagca tgatactttc
     2941 actataggcg caaattctag ctttgatttg aatgcggtta taaatgttgg agaggccaaa
     3001 aacaaaaata aatttgtaga atcatttatt cattttgagt cagtggaaga aatggaagct
     3061 ctaaactcca acgggaagaa aataaacttc caaccttctt tgtcgatgcc tctaatggga
     3121 tttgctggga attggaacca cgaaccaatc cttgataaat gggcttggga agaagggtca
     3181 agatcaaaaa cactgggagg ttatgatgat gatggtaaac cgaaaattcc aggaacctta
     3241 aataagggaa ttggtggaga acatggtata gataaattta atccagcagg agttatacaa
     3301 aatagaaaag ataaaaatac aacatccctg gatcaaaatc cagaattatt tgctttcaat
     3361 aaccaaggga tcaacgctcc atcatcaagt ggttctaaga ttgctaacat ttatccttta
     3421 gattcaaatg gaaatcctca agatgctcaa cttgaaagag gattaacacc ttctccactt
     3481 gtattaagaa gtgcagaaga aggattgatt tcaatagtaa atacaaataa agagggagaa
     3541 aatcaaagag acttaaaagt catttcgaga gaacacttta ttagaggaat tttaaattct
     3601 aaaagcaatg atgcaaaggg aatcaaatca tctaaactaa aagtttgggg tgacttgaag
     3661 tgggatggac tcatctataa tcctagaggt agagaagaaa atgcaccaga aagtaaggat
     3721 aatcaagatc ctgctactaa gataagaggt caatttgaac cgattgcgga aggtcaatat
     3781 ttctataaat ttaaatatag attaactaaa gattacccat ggcaggtttc ctatattcct
     3841 gtaaaaattg ataacaccgc ccctaagatt gtttcggttg atttttcaaa tcctgaaaaa
     3901 attaagttga ttacaaagga tacttatcat aaggtaaaag atcagtataa gaatgaaacg
     3961 ctatttgcga gagatcaaaa agaacatcct gaaaaatttg acgagattgc gaacgaagtt
     4021 tggtatgctg gcgccgctct tgttaatgaa gatggagagg ttgaaaaaaa tcttgaagta
     4081 acttacgcag gtgagggtca aggaagaaat agaaaacttg acaaagacgg aaataccatt
     4141 tatgaaatta aaggtgcagg agatttaaga ggaaaaatca ttgaagtcat tgcattagat
     4201 ggttctagca atttcacaaa gattcataga attaaatttg ctgatcaggc tgatgaaaag
     4261 gggatgattt cttattacct agtagatcct gacaaagatg cttctaaata tgaaaaactt
     4321 ggtgaaattt ctgaagataa actcaaaaat gcaaagagcc cagaggaaaa taccaataat
     4381 aatcaagcta aggatgaaga ttcaaaacca gatgaaaaaa gttcagttga gggagaagct
     4441 agccttgaaa taaataaaac tatttcaaca attagagagt ttgaaaataa agacctaaag
     4501 aaactcatta aaaagaaatt tagagaagtt gatgatttta caagtgaaac tggtaagaga
     4561 atagaggaat acgattataa atacgatgat aagagaaata ttattgctta tgacgatggt
     4621 agtgccttac aatatgaaac tgaaaaattt gacgaaataa aatcaaaaat ttatggtgtt
     4681 ctaagcccat ctaaagatgg acactttgaa attcttggaa agataagcaa tgtttctaaa
     4741 aatgccaagg tatattatgg aaatagctat aaatcgatag aaatcaaagc gactaagtat
     4801 gattcacatt caaaaacgat gatatttgat ttatacgcta atattaatga tattgtagat
     4861 ggattagctt ttgctggaga tatgagatta tttgttaaag atgacaatca aataaaagct
     4921 gaaactaaga ttagaatgcc tgaaaaaaat aaggaaacta aagcagaata tccctatgta
     4981 tcaagttatg ggaatgtaat cgaattagga gaaggagatc tttcaaagaa caaaccagac
     5041 aatttaacta aaatggaatc tggtaaaatc tattctgatt cagaaaaaca acaatatctg
     5101 ttaaaggata acatcattct aagaaaaggc tatgcactaa aagtgactac ctataatcct
     5161 ggaaaaacgg atatgttaga aggaaatgga gtctatagca aggaagatat agcaaaaata
     5221 caaaaggcca atcctaatct aagagtcctt tcagaaacaa caatttatgc tgatagtaga
     5281 aatgttgaag atggaagaag tacccaagct gtattaatgt cggctttgga cggctttaac
     5341 attataaggt atcaagtgtt tacatttaaa atgaacgata aaggggaagc tatcgataaa
     5401 gacggaaatc ttgtgacaga ttcttctaaa cttgtattat ttggtaagga tgataaagaa
     5461 tacactggag aggataagtc caatgtagaa gctataaaag aagatggctc catgttattt
     5521 attgatacca aaccagtaaa cctttcaatg gataagaact actttaatcc atctaaatct
     5581 aataaaattt atgtacgaaa tccagaattt tatttaagag gtaagatttc tgataagggt
     5641 ggttttaact gggaattgag agttaatgaa tcggttgtag ataattattt aatctacgga
     5701 gatttacaca ttgataacac tagagatttt aatattaagc tgaatgttaa agacggtgac
     5761 atcatggact ggggaatgaa agactataaa gcaaacggat ttccagataa ggtaacagat
     5821 atggatggaa atgtttatct tcaaactggc tatagcgatt tgaatgctaa agcagttgga
     5881 gtccactatc agtttttata tgataatgtt aaacctgaag taaacattga tcctaaggga
     5941 aatactagta tcgaatatgc tgatggaaaa tctgtagtct ttaacatcaa tgataaaaga
     6001 aataatggat tcgatggtga gattcaagaa caacatattt atgtaaatgg aaaagaat
//
LOCUS       DAVLXF010000100         6025 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLXF010000100 DAVLXF010000000
VERSION     DAVLXF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.100"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(179..799)
                     /locus_tag="VYO12_001863"
     CDS             complement(179..799)
                     /locus_tag="VYO12_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1588406.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKAPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEPTPEPGTPKTETPVNLDPEV
                     PTYETGKREGLSNTGTEANATLASAGIMTLLASLVLGFFKKKEDEK"
     gene            complement(757..1863)
                     /locus_tag="VYO12_001864"
     CDS             complement(757..1863)
                     /locus_tag="VYO12_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1588407.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVDSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(1974..2228)
                     /locus_tag="VYO12_001865"
     CDS             complement(1974..2228)
                     /locus_tag="VYO12_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588408.1"
                     /translation="MLNVDQDFMSISKSNKSDSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(2586..3119)
                     /locus_tag="VYO12_001866"
     CDS             complement(2586..3119)
                     /locus_tag="VYO12_001866"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1588409.1"
                     /translation="MTIFKEQESEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(3106..3432)
                     /locus_tag="VYO12_001867"
     CDS             complement(3106..3432)
                     /locus_tag="VYO12_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1588410.1"
                     /translation="MAYNTDFKQGALDSIKGGHRHVEAAKVFWCWRQNSLHVGKERRE
                     QRNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIAAHFDCAVPSVWAALKQIKVTL
                     KKDDDL"
     gene            3666..5384
                     /locus_tag="VYO12_001868"
     CDS             3666..5384
                     /locus_tag="VYO12_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1588411.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKEIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            5495..5842
                     /locus_tag="VYO12_001869"
     CDS             5495..5842
                     /locus_tag="VYO12_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1588412.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
BASE COUNT         1681 a         1245 c         1101 g         1998 t
ORIGIN      
        1 aatttttacc ctatttagca actgatttct ggaaatattt ttaaaaaaat aggattcata
       61 gtttcccagt tcaacggact tgtaagcgtt tttattcttc acgaaagacc tcgttctcaa
      121 aaccaggaaa aaagcccaca tctgtgagct tttcctggtt cctagattct aaaatctatt
      181 atttttcatc ttcttttttc ttgaagaatc ctaatactag actagctaac aaggtcatga
      241 taccagcact agccaaggta gcattagctt ctgtacctgt gtttgacaat ccctctctct
      301 tacctgtctc ataagtcgga acttctgggt ctagattcac tggagtttca gtttttggag
      361 tacctggttc tggagttggc tctggtattg gagtctcttg atttggtagt tctgggtttg
      421 gtgtcggaac acttggatgc tctggatttg gttgtgggat tggtttttct ggagttggtt
      481 tatctggtgt tgataaacgg tcatacctta ccgttatttc tttatcacta gagtctgacg
      541 taacttcttg tgattcaact gttggaatat ctggagcttt gtacttgtca atcttaccag
      601 atacaacctc gtcccagttt cctgttgtcc attcaccgta ggttacaact cccgtgacct
      661 tgttctcagt ttttgtacgg cttaaggtta caggttgaac aacatcttct tttacatttt
      721 ggttcgtaac tttatcaacg taatgaatga tacgcgttat agtcttcgtc tcagtagagg
      781 ttgctgtttt gggaaccact gtttcctcaa cattctcacg gtagtaatag tcaactgttg
      841 caccgtcttc tggtacgcat ttgcaggagt tgctaccaag gtgtatgttg ttttccttgt
      901 gataactcgg tcttctttgt cctcagttgt tgttttccct tcaatagttt ttgattctgt
      961 ggtatactca gaacctatcg ctaaatcagc ttttataaca gactctgcca acttctcttg
     1021 gctaccttct ttatagtaat tggctgttac tgtagcagtg gttggcgctt cgctttctct
     1081 ataaactaag gtcactgttc taccttcgct tacaatattc ccagttaaac ttgcagaatt
     1141 tgtatctgct tctttaaaag tataatattt tccgtcagta gtagtcatgc tactgagttt
     1201 tttatctgtg acattgtagc tggtaccaat cagttgtttt ttattggtaa tgtaggttcc
     1261 gtcactttct ttttctccaa ttccagtatc atttttcaat gatagcaaac gcccttgttc
     1321 atcaacatag cgaactttca cattttctga gattagttct gccaattctg aggttttttt
     1381 ctttttcttg atttcttcgg ttattttccc tttctcttct tcgggaatat ttagttttgg
     1441 aatgattttt tcaacaacgg ttcgtgatgg ttccacagta tcttggatga ctgaaaagtc
     1501 agctagaatt gggagattat actgaacacg gtgactttga gtgtttactc ctactctttc
     1561 attattctct gaaaatactt gtacggtata agaaacaaca tcttttccta atagaacatc
     1621 cccagtagag aaatagccgc cttttcctag tttgctatct ccagagtcca cttctttcct
     1681 aatcttatca gatagttttt taccagtcag tacattcgtt cgcacaatcc ctttgtctac
     1741 ccctacaaag tgggagaact ttttgaactc ttcagaacca gatctagccc aaccattatt
     1801 aagggcattt gcttttgtat ttgtattctc tctcaaaggt ttggcgatta gaattatatt
     1861 catcggcact tagagttgct gctatatctg actcttgaat accaacttcc ttactaccat
     1921 ttctagcggc agtatatgtg tatttatctg tttctacttc tagctgatta tccttaacat
     1981 gataggtaat tttctcctgg tataagagtc ttcagtagtt tatatgctct gtcatcatct
     2041 ttagctttag aagcagctga gtttagccaa gttggggtat taggagtatc tttatcagcc
     2101 tgtttcaaga cattattcaa gtcatttgct aacctattgg ttgtcctcac tgtgaaagtt
     2161 ttcttccagt ctgaatcact tttattagac ttagatatgc tcatgaaatc ctggtcaaca
     2221 ttcaacacaa tatcgccttg ttttttagca tcttccgttg cttctgcttc aaattgatca
     2281 tggagttttt gttgctctgg tgtatagttt ttttatttac acgatttaat gtactatttt
     2341 ttattttagt attaagaact ccattaatac gatttttaaa ttctgcttca aaaaatccct
     2401 taatttgttg gtgcgcaagt tcaacattca aacctttctt atacaagtct tcattctgat
     2461 acaagtcttc attctgaacg atttcggcta cacggtgaat ggtttgatcg tataccgtct
     2521 tctcttaacc agcagtatcc ttagttttaa tgactactcg ataggttcct ttagaaccgt
     2581 tatagtcatc tagttttaaa aaagcattcc aaacattcaa aaatagttgg aacttccctt
     2641 aataaatccg tcactttctt tttcaagata gcccaagctt gctcaatagg attcaaatcc
     2701 ggtgaataag gtggtagagg taagaaaaag tgtttatctt ggatgcagag ttcatccaaa
     2761 atgttcttgg gatgaaaact agcattgccc atgacaataa catgaggtgt cttcaaagca
     2821 ggtaggagtt gcgttttgaa ccactccaca aagaaatcgc ttgtcatgct tttcttgtaa
     2881 atcatgggag ctataaactc tccgtctact tgtcctgcaa caattgaagt tcgctcaaaa
     2941 cgacgtccgc taatcttttc atagactttc tcccctctag gagcccctgc ataaggacga
     3001 tagaggtagc ggtcgattcc tgtttcatca atatatacga ttggtaaatc ttttaggtta
     3061 tccaaaatat caagaaactc agcgactttc tctgactctt gttccttaaa gatcgtcatc
     3121 tttttttaaa gtgaccttaa tctgctttaa agctgcccat actgaaggaa cagcacaatc
     3181 aaaatgtgcc gcaatttccc gtaaaaaagc atctggatga gcctctacaa aggctttcaa
     3241 ttcttctaaa gggatcttac gctttttgac gactcgcttt ttccgctcta agttcctttg
     3301 ttcacgtctt tcttttccca cgtgaagaga gttctgacgc caacaccaaa aaactttggc
     3361 cgcctcgaca tgtctgtgcc ctcctttgat ggaatctaat gctccttgtt taaaatcagt
     3421 attatatgcc atagctttat tagaccatgc ttattttaaa ctaagcaact atatcaatgt
     3481 tttgttatga gaagacttac tttttttact gaaattgaat ttttcctagc ctcaacatca
     3541 tataacccca aaattcaatc tgacctggtt tgaagagatg ttcgaagcag ataaggtttt
     3601 actaaaacta aaaaaaatga tataatcaaa ctgattaaat gttttaaaaa taaaggagaa
     3661 tttgaatgtc ttaccaagaa aattaccaga aatgggttga ttttgtggag cttcctgact
     3721 accttcgtca agatttggaa aatatggacg aaaaaactaa ggaagatgcc ttctatacaa
     3781 atcttgaatt tggtactgca ggtatgcgtg gcttggttgg tgctggtaca aaccgtatca
     3841 acatctacgt tgttcgccaa gctactgaag gattggctcg tttgattgag tcaaaaggtg
     3901 gaaacgagaa agaacgcggt gtagcaattg cctacgatag ccgtcacttc tcacctgagt
     3961 ttgcctttga atctgcggca gttcttgcta aacacggcat caaatcttac gtatttgaaa
     4021 gccttcgtcc gactccagaa ctatcatttg cagttcgtca tctcaactgt ttcgcaggta
     4081 tcatggtcac agccagccac aaccctgctc catttaacgg ttacaaggtt tacggtgaag
     4141 acggtggaca aatgcctcca cacgatgcgg acgctttgac tacttatatc cgtgcaatcg
     4201 aaaacccatt tgcagttgaa gttgctgatg tggaaactga aaaagcttct ggcttgattg
     4261 aagttatcgg cgaagctgtt gacgtagaat accttaaaga ggttaaggac gtaaacatca
     4321 acccagcctt gattgaagaa tttggtaaag acatgaagat tgtctacaca ccacttcatg
     4381 gtactggtga aatgttggct cgtcgtgctc ttgcccaagc aggatttgac tctgttcaag
     4441 ttgttgaagc gcaagcaact gctgacccag acttctcaac tgtaacatct ccaaacccag
     4501 aaagccaagc agcctttgcc cttgctgaag aacttggtcg tcaagttggt gcagatgttc
     4561 ttgtcgcaac tgacccagac gctgaccgtg ttggtgttga agttcttcaa aaagatggta
     4621 gctacctcaa cctttcaggt aaccaaatcg gtgctatcat ggctaaatac atcttggaag
     4681 ctcacaaaaa cgctggaact cttcctgaaa atgccgctct ctgcaaatct atcgtttcaa
     4741 ctgacttggt aacgaagatt gctgaaagct acggcgcaac tatgttcaac gtcttgacag
     4801 gtttcaaatt tatcgctgaa aaaatccaag aattcgaaga aaaacacaac cacacttaca
     4861 tgatgggatt tgaagaaagc ttcggttact tgattaaacc atttgtacgt gataaagatg
     4921 ccatccaagc cgttcttgtc gttgctgaac ttgctgccta ctaccgttct cgtggtttga
     4981 cacttgctga cggtatcgaa gaaatctata aagaatatgg ctactacgca gaaaaaacaa
     5041 tctctgttac tctttcaggt gtcgatggtg ctgaacaaat caaagagatt atggctaaat
     5101 tccgcaacaa tgctccaaaa gaatggaacg caacagctat cactgtcgta gaagacttca
     5161 aggcacaaac tgctactgtt gctgacggta ctgttacaaa cttgacaact cctccaagtg
     5221 atgtgttgaa atacacactt gctgacggtt catggattgc cgttcgccct tcaggtacag
     5281 aaccaaaaat caagttctac attgcagttg taggggaaac caacgaagaa tcacaagcta
     5341 agattgctaa catcgaagca gaaatcaatg catttgtaaa ataaagacct ataaaagatg
     5401 agaccaacca acctcatctt ttttcgtatc aaatctaagc aattttagaa actttatggc
     5461 atactagact tatcaatgaa agcgaggtaa tctcatggaa caatttttag ataacatcaa
     5521 agaccttgaa gtcactacag ttgtgcgtgc gcaagaagct cttgataaaa aagaaactgc
     5581 aaccttcttt atcggtcgca aaacttgccc ttactgccgt aaatttgcag gtacattgtc
     5641 aggtgtcgta gctgaaacca aagctcacat ttacttcatc aatagtgaag aaccaagcca
     5701 actcaatgat ttgcaagcat tccgctcacg ttatggaatc ccaactgtac caggttttat
     5761 tcatattaca gatggacaaa tcaacgtccg ttgcgactct tcaatgtcag cacaagaaat
     5821 caaagatttc gcaggattgt aaaaaatcag ctaacaagtc ttaaaatcac aaaaacgcat
     5881 aatgtaaggt gcaaaaacct tgatattatg cgttttattg ttcctaaaac agttacatca
     5941 gtatttcatt acagaacatt ttaattttgg aagtcaaatc aatttttaac aatgttttag
     6001 aaatcacagt gggctattcc atatt
//
LOCUS       DAVLXF010000101         6006 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLXF010000101 DAVLXF010000000
VERSION     DAVLXF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.101"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(55..744)
                     /locus_tag="VYO12_001870"
     CDS             complement(55..744)
                     /locus_tag="VYO12_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1588413.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEIFKNEN"
     gene            complement(756..1634)
                     /locus_tag="VYO12_001871"
     CDS             complement(756..1634)
                     /locus_tag="VYO12_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1588414.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(1624..2493)
                     /gene="licA"
                     /locus_tag="VYO12_001872"
     CDS             complement(1624..2493)
                     /gene="licA"
                     /locus_tag="VYO12_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1588415.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIEFAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(2510..3532)
                     /locus_tag="VYO12_001873"
     CDS             complement(2510..3532)
                     /locus_tag="VYO12_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1588416.1"
                     /translation="MINQIYQLTKPKFINVKYQEEVIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(3537..4244)
                     /locus_tag="VYO12_001874"
     CDS             complement(3537..4244)
                     /locus_tag="VYO12_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1588417.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLHKERIIITKGGADRNTSIEKIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4580..>6006
                     /gene="tacF"
                     /locus_tag="VYO12_001875"
     CDS             4580..>6006
                     /gene="tacF"
                     /locus_tag="VYO12_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1588418.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKD"
BASE COUNT         1847 a         1237 c         1005 g         1917 t
ORIGIN      
        1 aaagttcgta aaaaatcaaa aaacatccga ctattttgtc agatgttgga atctttaatt
       61 ttcgttttta aaaatttctt ctaatttacg atagtcttgg acactatcga tctcataaat
      121 gctattgcct tctaattctt caacatagac atctagctct ttgatattat ccttaaccat
      181 attgtcccaa tagagatcaa caaattcgcc acttgcataa gccttatcga taaagctgac
      241 aatcttttct gcagttggag catcccagaa ggatacacca ctaaggatgc gacctgcctt
      301 gctatcaaca ataatgtctt gaaccttgta gtcatctcca tagaccaaga accattcgtt
      361 ggtacaatct tcacgataaa cactaaaata agtcgaacga gtcaaatcat tgcggaacat
      421 atttttaaag agataattgt cagcatcaat aacatagctg ttggccaatt cttcttttac
      481 aagatagaga gagtaaaagt tattgtagtc agcgtattta tcattgaaaa cgaggcgaac
      541 accgtatttt tcttttaagt aatcgaattg ttctttaaga taaccaacaa tgatgatgat
      601 gtcattgatt cctttttctt tgagaaactc aatttggtac tcaattaagg gtttttgatt
      661 aacctgaacc aaggctttag gggtattttc agtcatagga cgcaagcgag ttcccaatcc
      721 cgctgctaag ataatggctt tcacacgaat ctcctttatt ctttaataat aatataaact
      781 ccagcaatga cgacaagtga cgtcataatg gtcagcatat ctagcggtgc acccaagaaa
      841 acaactgcaa acaagaccgt ccatactaca tagctcacgt tcaagcctgt agccttggct
      901 ggttgcaagc gattgatagc gatataataa gccaagtagg aaatcatatc aaaggctgca
      961 aaaacaatca tgagacctag caattgtcca ttggctactg cagtaaatga ctgatgagag
     1021 aagagcacaa tcacaagata ggacaagaac gaagttactt gacggattaa gagggcttcg
     1081 atttcactca attcactttc catggcaaaa gagctaagaa cactctcact tccccatgca
     1141 atagcacaaa ccaaagcaca aagaatccca atgtagaaag aattaacctg ttcaacctta
     1201 taggtctgag caataatccc tccaataatc aagacaatcc caaatacagt atttttcgaa
     1261 atcttgtgct tcaaaaagaa gaaagccaat agaactgaaa tcgcagggta aatagccgat
     1321 acagatgaag ctaaagaact tccgatatac ttaactgcat aaagattggc ctgcataccg
     1381 atagggcctg ctagcaaggc tccgatgata acactgacat tgcgaatatt taagaaaatt
     1441 gagaggcgaa ctttcccttc ttttaccaag agaaaagcta gtaagataaa gatgctcaaa
     1501 aaatcatgag ctgcagccac cacaaagggt gacaaatctg taaaaatcga aaagatataa
     1561 gcactaaccg ttagacccaa gccccagaaa atacctgaga gaaggccaaa aggaactccg
     1621 tttttacttt tcatctgaac ctccataaga agccaaacct ttaacagctc tttggtaacg
     1681 attcacacca tagtcaccaa aatcttcacc ttgctcttcc ttatagacag tccatagact
     1741 ccaaatagta tcttgtaaaa ttttataaat agcaatcttt tcatgagaaa ccggtgtttg
     1801 gtcactctca tagtgagata agaaagtttc ttcctcttgg gaagtgaatt cagactctaa
     1861 aaagagggca gccaaatccc acattggatc attcattgat gaatattccc agtcaatcaa
     1921 ataaagtcgt ccttgaggtg attcgataaa gttttcaggc accaaatcga tatgacaaga
     1981 ttttctgtca acacctaagt cagccagtct tttctctaag gagaagactg catttctaac
     2041 agattcatag ttggcataag gaatttgttc ttcaatcaag gattcgtatt ttttgatttc
     2101 ttcaaaagga gcaaattctc ctcttaattc cttagcagac gtatgaatag tttgtaatat
     2161 tggagcaatt ttgtcgaact tggtcttgat tgacgttgaa tcaagcgtaa tcgcaaattc
     2221 gatatactca tttactttga taccagcttc aatatcaaaa agataatttt ttacatctaa
     2281 gcctaaatcc tttagtagtt caagattgta cttttcatct tgtcgattga taagcttttc
     2341 tgtcccttta ccaaagaatt taacaatgta ttgcttattt gttgttttgg ccaaatagtt
     2401 ttgattggtc attccaccca gttgttcaac actgaggact tcctcttctt gactaagtaa
     2461 ggaagaaatt ttttctttaa tgattttctc cacaattaac ctccagtact tatacttccc
     2521 acttaaacac tgttttaaag gctgtgttta aatcggttgc aaagacacga tgaatatctt
     2581 taatttctct tacaggttct tctagataaa ggatattttt aagacgattg gcaaatttct
     2641 tgacttccat catttggata gcattttcaa aatcaatgcg accagaacga gatgacccaa
     2701 ccaaaatcaa gcccttttct aaggcatcgc gagtattgag attgacttta tattcgctaa
     2761 ctcccatcat gagaatcgtt ccctgaggac gaatgtagcg aatcaagtca ttaatagctg
     2821 gtccagtacc atcaccacca caacattcaa aagcatggtc aaaggccaaa tcttcaggaa
     2881 tattatccgt aatatagcat tctttggcaa atgagaagag ttccaacttt tcccaatgac
     2941 gaccaataac cacaatctct gcttctggca aagtatagtt gataatattg gcaaccacaa
     3001 aagctaaact tccatctcca ataacggcga tccgctcccg cttgctatga gcaagagtca
     3061 atagacgatt catagcgtgc atgcccacac tgacaaactc tgtaatggct gcaaccgtat
     3121 cttcaatagc atcataagcc accacacgat ctttagggag agaaacaaac tctctcataa
     3181 agccatcaaa tccactagac aagaaatggg tccctgtcat gtagttttca tagaattctt
     3241 catcactctg cataggagac tgattgggaa tcatgacaac tttttgacca acctcgtagg
     3301 ttccggtcgg gtcagaaatg acggttccac atgactcgtg aatcattgcc attggaagct
     3361 ttttattcaa aatcttggga tcacgttttc cctgatagta acgctgatcc gcatgacaga
     3421 cagccatgta gttgggacgg ataaggatat gattctcttg gtcaataacc tcttcctgat
     3481 atttgacatt gataaactta ggcttagtta gttgataaat ttgattaatc attttactag
     3541 tctttctcaa tcatactttt tgcaatcttc aaatctgtta cggttgtaat cttcagattt
     3601 gagtattcac ctttggccaa ggccacatct tttcctttga tcacaaagat tttacatgca
     3661 tctgtcaaga tttccttctc ttcatcagaa agagatccat aaaggtccat gaagtccttg
     3721 caacggaatg tttgaggtgt ttgtccttga taaaggtgag cacgatttgg aatatctgta
     3781 ataaattgac cattggtact ttcaacgata gtatcaaccg cttctaccac tgtgtccact
     3841 gcgtcatgat tttgggcaag ttggatattg tcctgaatca tgcgaagtgt aataaatgga
     3901 cgaacagaat cgtgggtaac aacgatatcc tctgaagtaa gcggacgata agcatcaatg
     3961 gcttcaatga ttttctcaat acttgtattg cggtcagcac caccctttgt aatgatgata
     4021 cgttccttgt gaagaggaag atatttatct acaagatctt ctgcatgaga aacccagtct
     4081 ccatgaaccc caactacaat tttttcaata cttggctcca agacaaattt ttcaattgta
     4141 tgaatcaaaa taggtcgatc acctagctct aaaaattgtt ttggcaagtt actgattccc
     4201 atgcgtgtgc cagttccacc ggcaagaatt cctgcataaa tcatctattt tctcctttgt
     4261 cttactaaaa aaacttattc tctctattat accacaatct agtcctatca taaaagaaat
     4321 actggtcctc cccttctaga ataggagaat tatgcagttc tattattcaa agaaactagt
     4381 tcatttctag taatgactag gaattctgta gcaattacta ataaatggct gcgaaatttt
     4441 agagtccttc agaataatat actttcctta aagaaaactt aaaatttaag atttgaaact
     4501 tcaaagtgat tacttgactt actgcccttc atttcttatt agctaacttt atgatataat
     4561 gaaaaacgag gtaaattgaa tgaaaagtat aaaattaaat gctctatctt acatgggaat
     4621 tcgtgtcttg aatattattt ttcccatcct aactggaacc tatgtcgcgc gtgtcttgga
     4681 ccgaactgac tatggttact tcaactcagt cgacactatt ttgtcatttt tcttgccctt
     4741 tgcaacttat ggtgtctata actacggttt aagggctatc agtaatgtca aggataacaa
     4801 aaaagatctt aacagaacct tttctagtct tttttatttg tgcatcgctt gtacgatttt
     4861 gaccactgct gtctatatcc tagcctatcc tctcttcttt actgataatc caatcgtcaa
     4921 aaaggtctac cttgttatgg ggattcaact cattgcccag attttttcaa tcgaatgggt
     4981 caatgaagct ctggaaaatt acagttttct cttttacaaa actgccttca tccgtatcct
     5041 gatgctggtc tctattttct tatttgttaa aaatgaacac gatattgttg tctatacact
     5101 tgtgatgagt ttatcgacgc tgattaacta cctgattagt tatttttgga ttaaaagaga
     5161 catcaaactt gttaaaattc acctaagtga ttttaaacca ctctttctcc ctctgacagc
     5221 catgttagtc tttgccaatg ccaatatgct cttcactttt ttagatcgcc tcttcctcgt
     5281 taaaacaggg attgatgtca acgttagtta ctataccata gctcagcgaa ttgtgaccgt
     5341 tatagctggg gttgtaacag gtgcaattgg agtgagtgtg cctcgtctca gttactatct
     5401 ggggaaagga gacaaagaag cctatgtttc tctggttaat agaggtagtc gaatctttaa
     5461 cttctttatc attccactga gttttggact catggtttta ggaccaaatg ccatcctact
     5521 ttacggtagt gaaaaatata tcggaggcgg catcttgacc tctctcttcg cttttcgtac
     5581 gattatcctg gccttagata ccattcttgg ttcccaaatt ctctttacca atggctatga
     5641 aaaacgtatc acagtctata cagtctttgc tgggctactc aatttgggct tgaatagtct
     5701 cctctttttc aaccatatcg tggctcctga atactactta ctgacaacta tgttatcaga
     5761 gacttctcta cttgttttct atatcatttt catccataga aaacaactca tccacttggg
     5821 acatatcttt agctatactg ttcgatactc tctcttttca ctttcctttg tagcaattta
     5881 tttcctgatt aatttcatgt atcctgtgga tatggtcatt aatttgccat ttttgattaa
     5941 tactggtttg attgtcttgc tatcagctat ctcttatatt agtctacttg tcttcacaaa
     6001 agatag
//
LOCUS       DAVLXF010000102         5864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLXF010000102 DAVLXF010000000
VERSION     DAVLXF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.102"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..663
                     /locus_tag="VYO12_001876"
     CDS             <1..663
                     /locus_tag="VYO12_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1588419.1"
                     /translation="NIDCMAIGQYLFNPHNTPDNFIFLHAGLGIYTSFFTKEKIGASK
                     NPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFKNSQLTVLKSLVKT
                     EKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPADKIYINSQLLNYQP
                     FTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVAFFIEHSNVLQDII
                     SP"
     gene            1313..1483
                     /locus_tag="VYO12_001877"
     CDS             1313..1483
                     /locus_tag="VYO12_001877"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1588420.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            2095..2379
                     /locus_tag="VYO12_001878"
     CDS             2095..2379
                     /locus_tag="VYO12_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588421.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            2372..2620
                     /locus_tag="VYO12_001879"
     CDS             2372..2620
                     /locus_tag="VYO12_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588422.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLHATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            2716..2934
                     /locus_tag="VYO12_001880"
     CDS             2716..2934
                     /locus_tag="VYO12_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588423.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LESFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(3302..5626)
                     /gene="pflB"
                     /locus_tag="VYO12_001881"
     CDS             complement(3302..5626)
                     /gene="pflB"
                     /locus_tag="VYO12_001881"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1588424.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1916 a         1078 c         1010 g         1860 t
ORIGIN      
        1 aatatcgatt gtatggctat aggacaatac ctttttaatc cacacaatac ccccgataac
       61 tttattttcc tgcacgctgg attaggtatt tacacttcct ttttcacaaa agaaaaaata
      121 ggagcctcta aaaatcctta tatcggagaa attggacaca ccattgtcga attgaatggg
      181 caatattgtg aatgcggaaa aaaaggttgt ttacaaacat atatttcgga tgcttggtta
      241 atcaaacacg cccaattatt atttaaaaat tcccaactaa ctgtactaaa aagccttgta
      301 aagactgaaa aagacattca tttagacacc cttttaacgg cttataattt aggcgactcc
      361 gctttacgtc aacaaattga taaaggagtc aatttattag ccacttctat tgcaaatctc
      421 ctcctcatca atcctgctga taaaatctat atcaacagtc aattgcttaa ttatcaacct
      481 ttcactcatg aagtcaggga taaaatccaa gaccagctcc acttcgttcc ctttactcgt
      541 aatatagaaa ttgaaatttt accttacaac aaacatcgtg gaagtatagg agcttgtgca
      601 ttagctatcg tcgctttttt catagaacat agcaatgtat tacaagatat tatttcacct
      661 taatatatta gaaatctata gacctgttta aatcaactat aacctgtagt agatatctcg
      721 tatttagaca atatgaaaac aagacgactt ccatatagga aaccgccttc tctctatgtt
      781 gagtgattta tattaaaata acttttcttc tagctgcatt ttattattat aaaaacattc
      841 atcataaccc tgaacttaaa taacgatttt tattcaagat acatactcct agaataaact
      901 ttatatgaaa ttctcatttt tgtttttaca attctcctta gttaaatctt gtttaatata
      961 tgttttacat atagtattta gcgccacata gtactgaact ctctccaaaa acggttattc
     1021 ctctttgaat agggcgttat cacaagaaaa gcatctccac gtttcaactt catatggctc
     1081 aaaaacaatc aattgatgct aaaacctgta cctagatgtt tcggttcata aaaccatgaa
     1141 actgtaaaag tggatgaaat tgatagcgat agtcaaatca agaggcatca taactctaaa
     1201 aagtcacaat atataagttc atcctcggaa aaatatcatt ctaattgttg aaatgcctac
     1261 atgaaaagaa acgtcaaata ctcatgaaac aacgaataca ggtatcaaaa ctatgacaaa
     1321 acaagtccct aaatttacta aagacactgc tcaactttac acctgtaaat ggttgttgta
     1381 taataaagtt acaaagatgt acgaccacac tgttgtaaat catagtgttc gcgaatatat
     1441 tactgatagc atttctacaa atacaattga agagactgga tagactttaa acgaaatatg
     1501 tcagtgtttt ggcattccta gccttcatat catttaaaga attctataga caaaattttt
     1561 cccaatacag acactcgtaa caactgcttc atttttctac caacatattt aggaacagga
     1621 taagatacaa gaggattaat ccatagatca gttctatacc aatctaagac aaataagcta
     1681 aaaaaacgat tgataataag caaatagatt ccaaattttc tctatctgct cattttaata
     1741 aacaatacta gtgtaactat ccttccagtc agaagcttgt caaatcacac cgaaaattct
     1801 tctaaaattt atctcgttag gcaatcaagc aaaaactcga cgatagtaca aacattatca
     1861 tacagaattg acttcctaaa ttatatactt tagtaaggtt ttcgtataag aaaaaagatt
     1921 cattttacat ttctaaacat tcttttctaa gatgaaaaac agaatttttc gattgtgatt
     1981 taaagcaaca agaagatttt cagtatcatc ctatagatac gagctaatta agaaaaacta
     2041 cattttcgaa tataaactac aataatataa actaaatttt ataggaggaa gacaatggat
     2101 tggtacgatt atatgataca ggcatccaaa caatcacaat tcaacgcaag ccattggttt
     2161 cgctatttgc gaaaagttat ttttgaagac tattcttatt taacaaacca agatgtagaa
     2221 aagttgctag actccaaaga actaacccgt tttcaaaaaa ttagcttgaa gtatgccttt
     2281 caagagcata ctccaactca taaatatgtg atttcattaa ataaacctgc taagttaacc
     2341 aatgttcaaa aattgatgga gaaatacaaa catggataaa atgaaaccgg tcttccaagc
     2401 cctaaataag gaattaattc aggaaaatct gactttaaca attatctgtg tcgatggtta
     2461 tgtcttagaa tatcatggtt tacatgccac acaagatgtt gatgcttttt atgaccaaaa
     2521 tcagaaaatc aatgaaatta ttgctcgtgt aggaaaacag tttaatctta acatccacga
     2581 agagttatgg ctcaacaatc atgttgcaaa gcaaatatga acaaacaacc tcccttaagt
     2641 ttatgcgaat ccttgtactc atttgagaat ctgaccgttt tagtagtccc tattgagtat
     2701 gtgttaggaa tgaagatgat gagtatcaga gagcaggatt tgcaagatat tggtgcaatt
     2761 attaaataca aaaactttca ttcaccattc gataccttta aatatttaaa agacatggga
     2821 tttgatacta ttgacctctc tgttctatta gaaagtttta gttatgctta tggtatggat
     2881 tggttagaaa aattttttaa agaaaatcaa gataaacttc gcgaatttta ttaacagtat
     2941 caaacaagat gattgtcaat tagccagtcg tcttgttttt tatgatgaaa tgaaataaaa
     3001 tctgaacaaa tcgcttagag aattccagtc aatttctagc gctgtttgag acgtttcagt
     3061 ggactatagt aaaatgaaat aagaatagga caaatcgatc aggacagtca aatcgatttc
     3121 taagaatgtt ttagaaataa aggtgtacta ttctagtttc aatctattat atttcagatt
     3181 cggtccacta ttccttccca aaaacattgc atattgcgcc gaaggaattg ctcatactat
     3241 cccaagcaca aaaccctcag tcgtttgacc gagggttcct ttttattatt caagaacttg
     3301 attagctcaa tgcatccaag gcgtcatcca ttgaaagaac ttcgtggaag acacgttgtg
     3361 tcaattcagt tttttgttct ggagtgaggt atttagtgtt tacacagtat ccagagatac
     3421 gtacgataac gtcttcgcct gacatgattt tttcgtaaac atcgttcaag tccataacgt
     3481 tcaagttaac gtgttgtcca ccgttttcga agtaaccatc aaggattgtt accaagttat
     3541 caacttgttc atcacgagtc ttaccaagag cgcgaggtga tacttgtgta gtcaatgaga
     3601 taccgtcagc tgcataacta aagtcaaggc tagaaagtga gttcaagttt tgcaaccaac
     3661 cacctttagc tttgttagat gggttagcac ctggtgagaa gaattcaagt ttagacaagt
     3721 tcacagaacc atcttcgttg aggtatacac ctttgtgaac tggtgagtta ccagtttgtt
     3781 tagagtaagc aacgttagat gtgattgtca aaagtgatac tgtagcttct gcgtctttgt
     3841 atagtttgtg gctacgtaga cgagttgtgt aagcttcgat caaccattct gccaattcgt
     3901 ttgaacgtgg gtcatcttca ccccagcgtg ggtagtcacc gattgtttcg taatcgtaga
     3961 tgtagccatc ttcgtcacgg attggtttaa ctgtagcgta tttgatagct gacaatgtat
     4021 caacagtgtt agcaaatcca cagataccga atcccatgtt ggcacgttgt ttagttggca
     4081 agaaggccat ttgaacagct tcgtagttgt acctatcagt catgtagtgg atgatgttca
     4141 aggcatctac gtaagtgtca gtcaaccagt caagagattt ttcaaagttc gctttaactg
     4201 attcgaattc aagaacttcg tcacggattg gttcgatatc aaatactttg tagtctttgt
     4261 gaacatcgtc gtaaccacca ttcaaaccag taagaagggc tttaagaacg tttacacgag
     4321 caccgaagta ctggatgttg tggcgttgtt cttcattttc tggatcaagt ggagacacac
     4381 agcatgagat acagctcatt tcaccatatc cgtctttagc cattgttgtt acaccttcgt
     4441 attggataga agagtgtttg tggctcatgt gcatacagta gcgacggaag ttgtatggca
     4501 atttgtcagt ccaaagaact gtcaagtttg gttctggtga gttaccgatg ttgtcaagag
     4561 tgttcaagaa acggtagtcc atcttagtaa cacggtgacg accgtcgtta cccataccag
     4621 ccatagaagt tgtgataaag gttgggtcac ctgagtacaa ttggtcataa gctttggtac
     4681 gagcaaattt aactgtacga agtttcataa cgaaatcatc aacgaattct tggatttctg
     4741 attcagtaaa tgtaccacga gcaaggtcac gttctgcaaa gatgtccaat acgattggta
     4801 cacgacctag agatgtagca gcaccgttaa tcacacggca gacagccatg aaagcaatgt
     4861 taacccattg gattgcttct ttcacgttca tcgctggttt gcgaacatca accccgtaaa
     4921 ggtcacccag gcgaacaact tgttgcaatg cttggtattg aaggtttact tcttcacgaa
     4981 gacggattgt ttcttcatcg atttctttga ttgcattcca gtcatttact ttttcttgca
     5041 tcaagtagtc tgcaccgtaa agagcaagac gtgcgtaaac accgatgata cgtccgcgtg
     5101 agtatgcatc tggaagacca gttacagtgt gtgcgtgacg agcgcgacga atatttgaag
     5161 tgtaggcacg gaaaataccg tcgttaactg ttgttacata tttagtgaag atttcgtgaa
     5221 cagctgggtc tggttcgtat ccattttctt tcaaagtagt ttcagccata cggataccac
     5281 cttttggcat gaagttcaat ttgaagagtt catcgttttg gataccgaag ataacttcat
     5341 tttctttgtc gataaatcca gcagggatat cagcgataga tgttggacga gtgtccattg
     5401 ggaaacgagt ttcttcgtag tgtgctttag tttcttctac aattttcttg atgtgaagtg
     5461 aacgctctgt tggtcctgca aggaagcttt cgtctccatc ataaggtgtg tagttagctt
     5521 gtacaaatcg tgatacactt gctttttctt tccaatctac gcctttgaag ccttcccaag
     5581 ctttgtcaaa aatatcttgt gcttcaacaa ctgtcttaac aaccatatta atgtcctctt
     5641 ttttctagta acagtcatct gttacattca taagacaagt ataccatata gtaaccgatt
     5701 tcaacaagtg aaaattccct atttttacac tttcttttct aaaacagtct atattttatt
     5761 tcaaactgta ttatattttt gaaaaaataa agtccttttt tctttttttc agaaaaaagg
     5821 gtataataaa agaaaataag cagtaacact caatggaaat cgaa
//
LOCUS       DAVLXF010000103         5738 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLXF010000103 DAVLXF010000000
VERSION     DAVLXF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.103"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            7..960
                     /locus_tag="VYO12_001882"
     CDS             7..960
                     /locus_tag="VYO12_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1588425.1"
                     /translation="MKNKRIFKDFQASKMNLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1037..3025
                     /gene="uvrB"
                     /locus_tag="VYO12_001883"
     CDS             1037..3025
                     /gene="uvrB"
                     /locus_tag="VYO12_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1588426.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3035..3591
                     /locus_tag="VYO12_001884"
                     /pseudo
     CDS             3035..3591
                     /locus_tag="VYO12_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            3557..4066
                     /locus_tag="VYO12_001885"
     CDS             3557..4066
                     /locus_tag="VYO12_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1588427.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SKIILKSRS"
     gene            4119..4586
                     /locus_tag="VYO12_001886"
     CDS             4119..4586
                     /locus_tag="VYO12_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1588428.1"
                     /translation="MSRSQLTILTNICLIEDLETQCVVMQYRAPETNRWSGYAFPGGH
                     VENGEAFAESVIREIYEETGLTIQNPQLVGVKNWPLDTGGRYIVFCYKATEFTGTLQS
                     SDEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(4583..5098)
                     /locus_tag="VYO12_001887"
     CDS             complement(4583..5098)
                     /locus_tag="VYO12_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1588429.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHQVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5110..5637)
                     /locus_tag="VYO12_001888"
     CDS             complement(5110..5637)
                     /locus_tag="VYO12_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1588430.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
BASE COUNT         1727 a         1029 c         1301 g         1681 t
ORIGIN      
        1 ggggatatga agaataaaag aatatttaaa gacttccaag cttcaaaaat gaatttaaac
       61 atttacacaa gccccttgtt agcctttgtt tttgtcttca taggagagtt tgtggcttat
      121 actttatatg gtattagttt gttagctctc atcggacttg ctagaaattt tggagaggct
      181 ggtcaaaatc ttgcaagcta cttgcagacc ttgcatcaga gcttgacgga taaaacaagt
      241 gactttcgtt taattttagg attactggcc tttggtttta ttcttaacac tgtgttcaga
      301 tggacaagaa aagttgagaa aagacctatt cgaaccttgg gattttatag agagaatttc
      361 ctcagcaatc ttctgaaagg atttagtcta ggcctggcac tttttcttct gaccttgtta
      421 ggtttagtgg tcttaggtca atatcgtttg gaatccattc acttgaatcc ttattctctt
      481 gcctttgtcg tctttactat cccattttgg attttacagg ggacagcaga agaagtggtg
      541 gcccgtgctt ggctacttcc tcaattggcc tcaagaacca atctaaaact agctattctt
      601 atatctagcc tgttctttac cctgcttcat atgggcaatt ctggcctcac ccctctatct
      661 ctagtaaatc tctttttatt cggagttgcc atggctcttt accttctcaa aactgataca
      721 gtttggggtg ttgcaggtat tcatggtgct tggaattttg ctcagggtaa tctctttggg
      781 attttagtta gtggtcaacc gtcaggaacg tctctgatga cctttttacc acaaggcaat
      841 caagattggc tatcaggtgg ttcttttggc atagaaggtt ccattatgac aagtctggta
      901 ttactactgc tgattgtcta tcttgctaat aaattaaaga aagaaaatga aaggatgtga
      961 cttcggtccg tccttttctt cgtgaaaata ctataagtat gctaaaatag gaatagcaca
     1021 tggagagagg attcttatga tcaatcacat tacagataat caatttaaac tagtatcaaa
     1081 atatcaacca tcaggagatc aaccccaagc tatcgagcag ttggtggata acattgaggg
     1141 gggagaaaaa gctcagattc tgatgggggc gactggaaca gggaagacct atactatgag
     1201 tcaggtcatt tctaaagtca ataaaccaac tctggttatt gcccacaata aaactctggc
     1261 tggtcagctc tatggggagt ttaaggaatt tttccctgaa aatgcagttg agtatttcgt
     1321 atcctactat gattattacc agccagaggc ctatgtccct tctagcgata cctatattga
     1381 gaaggatagt tctgtcaatg acgagattga caagcttcgc cactcagcta cctcagccct
     1441 tttggagcgt aatgatgtta ttgtcgtggc ctcagtctct tgtatctatg gtttgggttc
     1501 gcccaaggaa tacgctgata gtgtcgttag tctccgtcct ggtctagaga tttctcgtga
     1561 taaactcttg aatgacttgg tcgatattca gtttgaacgc aatgatattg atttccaacg
     1621 cggaagattt cgcgttcgtg gggatgtggt agagattttc ccagcttccc gagatgaaca
     1681 tgcctttcga gtagaatttt ttggagacga aattgaccgt attcgtgaag ttgaggctct
     1741 gacaggtcag gtgttgggag aagtggatca tttagcgatt ttcccagcga cacactttgt
     1801 gaccaatgac gaccacatgg aagttgccat tgcaaagatt caggccgagt tggaagaaca
     1861 attagctgtc tttgaaaagg aaggtaaact gcttgaagcc cagcgtttga aacagcggac
     1921 agagtatgat atcgaaatgt tgcgtgagat gggctatacc aatggggttg aaaattattc
     1981 tcgccacatg gatggacgga gcgaaggaga gcctccttat acgcttctcg acttcttccc
     2041 agatgatttc ttgattatga ttgacgagag tcatatgacc atagggcaaa tcaagggcat
     2101 gtacaatgga gaccgttcgc gtaaagaaat gctggttaat tatggtttcc gtttgccgtc
     2161 tgctttggac aatcgtcctc tacgtcggga ggagtttgag agtcacgttc atcagattgt
     2221 ttacgtttca gcgacacctg gtgactatga aaatgaacag accgagacag tgattgagca
     2281 aatcattcgt ccaacgggac tcttggatcc agaggtggaa gtccgtccga ctatgggaca
     2341 gattgatgac ctcttgggtg aaatcaatgc ccgcgttgaa aaaaatgagc gtacctttat
     2401 cacaactttg accaagaaaa tggcagagga cttgaccgac tacttcaagg aaatgggtat
     2461 caaggtcaag tacatgcact cggatatcaa gaccttggaa cggacggaga ttatccgtga
     2521 cctgcgcttg ggtgtctttg atgtcttggt cggaattaac ctgctccgtg aaggaattga
     2581 cgttcctgaa gtgagcctcg tagctattct cgatgctgac aaggaaggtt tccttcgcaa
     2641 cgaacgtgga ctcatccaga ctattggacg tgctgctcgt aacagtgaag gccatgtcat
     2701 catgtatgca gacacggtta cccagtctat gcaacgtgct atcgatgaaa ctgcccgccg
     2761 tcggaaaatt cagatggcct ataatgaaga gcatggtatc gtaccacaga caatcaagaa
     2821 agaaatccgt gacctgattg ccgtgaccaa ggcagttgct aaggaagagg acaaggaagt
     2881 cgacatcaat agcctcaaca aacaagagcg caaagaactc gtcaagaaac tagaaaaaca
     2941 aatgcaagaa gccgtcgaag tgcttgactt tgaactggcg gctcagatcc gtgatatgat
     3001 gttggaagtc aaggccttgg attaggggaa tagtatgatt tatttaagaa agttaaagag
     3061 agaagatttg atgtctttat gggaaatggc ttattcacaa cttaatccag tttggaaaca
     3121 gtatgatgct ccctattatg atgattatca gtatttttca aattttaaag aattcgaact
     3181 acaaaaatca gaatccattt taagcaactc aaatcgcctt ggtatttttg ttgatgataa
     3241 actagttggg actgtttcgc gttattgggt atgtaaacaa acaagatgga tggaattggt
     3301 atttatgata aaaaattctg gaacactggt attgggaaag ttgctatgtt gcagtggata
     3361 gataggacgt ttcaggatta cttggagttg gagcatctgg gtttgacaac ttggtcagga
     3421 aatcttggta tgatgaaact tgctgaaaaa ttaagaatga aaaaagaagc tcatattcca
     3481 aaagttcgtt attatcaagg taaatatttt gatagtatta aatatggtat tttgagagaa
     3541 gactgggaga aaatgaatga cggttattat caaatcaatg gaaactcctg aagagataga
     3601 aggtaaatcc cttgttcact ggcaaacgtg gagagaggct tatgatgacc ttttgcctgc
     3661 agaatttcag gagacaatga cattagaaag atgtcgattc tttagtcaaa agtatccaga
     3721 aaatacattg attgcgatag gtgatctgaa ggtagttggt tttatcagtt atggtaactt
     3781 tcgtgatgag actattcaag ctggtgaaat tattgcttta tatgttttaa aagactatta
     3841 cggaaaaggc atagcacaaa agttaatgaa agcagctttg actgctctga atcatttttc
     3901 tgaaattttc ttatgggtat tgaaagataa caagcgagcc attgctttct atcaaaaaat
     3961 gggttttact tttgatggac aagaaaagat acttgaactt ggaaagccta taaaggaaaa
     4021 acgaatggta ttctattcta aaataatttt aaaaagtaga agctaatggc actggtacca
     4081 agtctgaaaa tttaataaat tagaaagcga gtaaatttat gtcccgttcc caattaacga
     4141 ttttaacaaa tatttgtctg attgaagacc ttgaaactca gtgcgtggtt atgcagtacc
     4201 gtgctcctga aaccaatcgc tggtctggtt atgcctttcc tggaggtcat gtagaaaatg
     4261 gtgaggcttt tgcggagtct gtcattcgtg aaatctacga agaaacagga ttgactatcc
     4321 aaaatccgca actggtaggt gttaaaaatt ggcctttgga tacagggggg cgctacattg
     4381 tcttttgtta caaggcgacc gagtttactg gaactcttca atcttcagac gagggagaag
     4441 tttcttgggt gcaaaaagac cagattccaa acttgaatct ggcctatgat atgctaccat
     4501 tgatggaaat gatggaagct cccgacaagt cagagttttt ctaccctcgc cgtacagaag
     4561 acgattggga aaagaaaatc ttctagtctt ttactaaata acctagctga tccaaggctt
     4621 cctcgatata gtggaggtct tgttgtgttt cggcttcgac taggtggtaa tggatgccat
     4681 ctgtcaattc agatagaggt ctaaagtcag aatgctcgac ttgttctaga aaatgttgca
     4741 catctctgcg acaagacagt ttaagcaagg tttcaatttc tccataaacg ggatggtcaa
     4801 tcaaggtatt ctggacatgc ccaccattat cgacaatagc aaggagttcc tgaccgattt
     4861 cttcaacttg atgtttcact ttgaaaagtt tgtgaacata aggattggta tcaatttgct
     4921 tatagatgta gccacgattg gtggatagga taggagcgcc atcggctctc aaaattgcaa
     4981 tgtcctgcac aatgacctga cgtgtgacat gaaaatgttc cgccaaactt tggccattaa
     5041 gggctttagg agcttctttc aagagttgga gcagggcttg tttgcgatct tttgtcatag
     5101 cttttccttt taacggcgtt ttcgaagcac tttatagaca gctagtgcta atgtatagtc
     5161 taccatacta tggataattg tgccaaatcc aactagtaca aatagaacat agaacatgtt
     5221 ttctacattg gtacccgaag ttgcgtaaaa aaggacacag gctaatactt cagcaagggc
     5281 atgaacaaca gccaaaacaa aattgaaaac ccaggaagat tttggtttat ctagggtatc
     5341 ggggaatttt tgtaggtaaa gagctcctaa agcaccaaaa gatatatggg aaaaagcccg
     5401 aaaaacgata accatgggat agccagccat caaaaatcca aaactagagg ctaggatgac
     5461 aaaaactgcc atcaagggcg acaagaacat ggctataaaa atagcgatgt ggctccccaa
     5521 agtataggaa gcaggtggaa tgacaatctt gaaaggcata acaattggaa tcaaaatcgc
     5581 aatagccgtt aaaagggctg tcattgtcat aaattgtgtc tttttccgtg tattcacaag
     5641 aatctccttt ttaactgcat atacactagt atggtacaat aaaccagaca ataaagcaag
     5701 aatttacttg ggtttataga tcatttttta gttaaaag
//
LOCUS       DAVLXF010000104         5547 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLXF010000104 DAVLXF010000000
VERSION     DAVLXF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.104"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            236..2002
                     /locus_tag="VYO12_001889"
     CDS             236..2002
                     /locus_tag="VYO12_001889"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1588431.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYCACQLLGPLHK"
     gene            2148..3299
                     /locus_tag="VYO12_001890"
     CDS             2148..3299
                     /locus_tag="VYO12_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1588432.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            3444..4328
                     /locus_tag="VYO12_001891"
     CDS             3444..4328
                     /locus_tag="VYO12_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1588433.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(4365..4613)
                     /locus_tag="VYO12_001892"
     CDS             complement(4365..4613)
                     /locus_tag="VYO12_001892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588434.1"
                     /translation="MKIKEQTRKLATGCSKHCFEVVDKTDEVSSKHCFEVVDRTDEVS
                     SKHCFEVVDRTDEVSSKHCFEVADRTDEVSNIYTARRR"
     gene            4817..5068
                     /locus_tag="VYO12_001893"
     CDS             4817..5068
                     /locus_tag="VYO12_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1588435.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            5056..5346
                     /locus_tag="VYO12_001894"
     CDS             5056..5346
                     /locus_tag="VYO12_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588436.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
BASE COUNT         1769 a          976 c         1207 g         1595 t
ORIGIN      
        1 tatgaagttt ttacacaaca tattgacagt ttagaaagca ataatctatc catagactta
       61 aaaggactga agggaaaaaa ggttagaatt tctttgagaa gcgcaggaat tcctttaagt
      121 ttagcagagg tagaggttta tacttataag taattttaaa aattatcacc caggctaccg
      181 taaatataat ggagatggta gtatgaaaga aacagaaaaa taagaggaaa atagtatgat
      241 tcaacatcca cgtattggga ttcgtccgac tattgatggt cgtcgtcaag gtgtacgcga
      301 atcacttgaa gtgcaaacaa tgaacatggc taaaagtgtg gcagatttga tttcaagcac
      361 attgaaatat ccagatgggg aacctgtgga atgcgtgatt tctccatcta ctattggccg
      421 tgtaccagag gctgcagctt cccatgagtt gtttaaaaaa tcaaatgttt gcgcaacaat
      481 tacagttaca ccatgctggt gttatggtag tgaaactatg gatatgtctc cagatattcc
      541 tcatgctatt tggggattta atgggacaga acgcccagga gctgtctatc ttgcagctgt
      601 actagcttca catgctcaaa aagggattcc agcctttggg atttatggaa gagatgttca
      661 ggaagctagt gacacagata ttccagaaga tgtcaaagaa aaacttttac gctatgcgcg
      721 tgcagctctt gcaactggct tgatgagaga cactgcttac ctatcaatgg gtagtgtttc
      781 gatggggatt ggtggttcta ttgtaaatcc ggatttcttc caagaatacc taggaatgcg
      841 aaatgaatcg gtagatatga cggagttcac gcgccgtatg gaccgtagta tttacgaccc
      901 tgaagagttc gaacgtgcgc tcaaatgggt gaaagaaaac gtaaaagaag gattcgacca
      961 taaccgtgaa gaccttgttt taagccgtga agaaaaagat agacaatggg aatttgttat
     1021 taagatgttc atgattggac gtgacttaat ggttggtaac ccaagacttg ctgaacttgg
     1081 ttttgaggaa gaagcagttg gtcaccatgc tttagtagct ggtttccaag gtcaacgtca
     1141 gtggacagac cattttccaa atggagattt tatggaaact ttcctcaata ctcagtttga
     1201 ctggaatggt attcgaaaac catttgtatt tgcgacagag aatgattcac taaatggtgt
     1261 gtctatgctc tttaattatc tattaacaaa tactccacaa atctttgctg atgtgcgtac
     1321 ttattggagc ccagaggctg ttaaacgtgt aacgggacat actttagagg gtcgtgctgc
     1381 agctggcttc ttacatctaa tcaactctgg ttcttgtaca ttggatggta caggtcaagc
     1441 tactcgagat ggcaaacctg ttatgaaacc attctgggag ttggaagaaa gtgaagtgca
     1501 ggctatgctt gaaaatacag acttcccacc agcaaaccgc gaatacttcc gtggaggagg
     1561 attctcaact cgtttcttga cgaaggggga tatgccagta acaatggtac gtctcaatct
     1621 tctaaaaggg gttggtccag tgctacaaat tgcagaaggt tatacacttg aacttcctga
     1681 agatgttcac catactttag ataatcgtac agatccagga tggccaacta cttggtttgc
     1741 tccacgtttg acaggaaaag gtgctttcaa gtctgtctat gacgtcatga ataattgggg
     1801 agctaatcac ggagccataa catatggaca cattggagca gacttgatta ccttggcttc
     1861 tatgttgaga attcctgtca atatgcataa tgtacctgag gaagatatct ttagacctaa
     1921 aaattggtcc ttatttggaa cagaagatct agaatcagca gactattgtg catgtcagtt
     1981 gttgggacca ctacataaat aaaacttgtt tatataggag gtgaacttac gtccctccta
     2041 tccttttaaa aagatttgtt aaacaattca caaataatta aaaacgaata caaaaagtaa
     2101 tataatgatg ttaaatagat agcgcggagg cgcaggagga aaattatatg gctacatttt
     2161 atgttccggc agtcaacctt attggaaaag gtgttgtaaa tgaagtgggt ccttatatca
     2221 aggaacttgg ctataaaaag gcacttttgg ttacagataa gtacatcgaa ggcagtgata
     2281 ttttacctaa gactttaaaa ccactggata cagaaggaat cgaatatgtc atctttagcg
     2341 atgtagagcc aaaccctact tgtaaaaatg tcacagatgg ggtagctgct ttgcaagaac
     2401 atggctgtga ctttatcatc agtcttggcg ggggttctcc acaggatgca gctagttgta
     2461 tttctatcat ggctacaaat ggtggaaaac cacaggatta tgaagggctt cataagtctg
     2521 ctaaaaaagg cttgccagtt gtggctatca atacaacggc aggaacttct gcagaaatca
     2581 ctattaacta tgtgattact gatgaagaac gcaaggttaa gatggtaatg gttgacaaga
     2641 atagccttgc tcttatctct gttaatgacc cggaactcat gctttccaaa cctaaaggcc
     2701 tgactgctgc tactggtatg gatgctctga ctcacgctgt tgaagctttg gtaacacctg
     2761 gtgcttatga tgtaaccaag aaactgtcta ttggtgctat tgagcttatc aaggaatatc
     2821 ttcctcgtgc tgtagaaaat ggacatgata ttgaagcgcg tgaaggtatg gtcaatgcca
     2881 tcttccttgg tggtatgagc tttaataatg ctggtcttgg ttatgttcac tcaatggctc
     2941 accaactcgg tgcagtatat aatttgccac atggcgtgtg ctgtgccatg ttgctaccag
     3001 ttatagaacg tgaaaatgct aaacgtgtac cagaagcttt ccgcaatgtt gccaaagcct
     3061 tgggacttca tgtagaaggt aaatcagatc aagaatgtgc cgattatgcg attgctgaga
     3121 ttgagaagct ttctgagaca gtaggtattc ctaagaaact gactgaactt ggtattgaag
     3181 aaaaagattt cgactttgaa tacctttcta agaatgcctt gattgatgcc tgcgcaccag
     3241 gaaatccatt tatgccaacc ttagaagaaa cgattgcctt ttataaagag ttattttagc
     3301 aagagattgg acgacaatag gacaattcac ctctaattat tgacatttca ggaaaaatcg
     3361 cagtaaaaac ggcacagcct tctttaaatg tgctataata ctagaaaaat acttgtggag
     3421 gttccattat ggcaatattt ttcatgattt ttctgattgt ttgtgtgctc ctattggtga
     3481 tagtcacact gagtacagtt tatgtggttc gtcagcagtc ggtggcgatt attgaacgct
     3541 ttgggaaata ccaaaaggtt gctaatagcg gtattcatat tcgcttgcct tttgggattg
     3601 actcgattgc agcacggatt cagttgcgct tgttgcaaag tgatattgtg gttgagacta
     3661 agaccaagga caatgtgttc gttatgatga atgtagcgac tcagtaccgt gtcaacgagc
     3721 agagcgtgac agatgcttac tataaactca tacgtccaga atctcagatt aaatcttata
     3781 tcgaagatgc tcttcgctct tctgttccaa aattaacctt ggatgaattg tttgagaaaa
     3841 aagatgagat tgcccttgag gttcaacacc aagtagcaga agaaatgacc acttacggct
     3901 acattatcgt gaaaaccttg attaccaagg tcgaaccaga tgcagaagtt aagcaatcca
     3961 tgaatgaaat caatgcggcg caacgtaagc gggtcgcagc acaagaattg gcggaagctg
     4021 acaagattaa aattgtcact gcagctgaag cagaagcaga aaaagaccgc cttcatggtg
     4081 tggggattgc ccaacaacgt aaggcgattg tggatggatt ggcagagtct atcaccgaac
     4141 tcaaggaagc caatgttggc atgacagaag aacaaatcat gtctatcctc ttgaccaacc
     4201 agtatttgga taccttgaat acctttgcct ctaaaggaaa tcaaaccatc tttttaccaa
     4261 atactccaaa tggtgtggat gatatccgaa cacaaatctt gtcagccctt cgcgctgaga
     4321 agaaataata gactaatact cttcgaaaat ctcttcaaac cacgtcagcg tcgccttgcc
     4381 gtatagatgt tactgacttc gtcagttcta tctgcaacct caaaacagtg ttttgagctg
     4441 acttcgtcag ttctatccac aacctcaaaa cagtgttttg agctgacttc gtcagttcta
     4501 tccacaacct caaaacagtg ttttgagctg acttcgtcag tcttatctac tacctcaaaa
     4561 cagtgttttg agcaacctgt ggctagtttc ctagtttgct ctttgatttt cattgagtac
     4621 aaagagcttg gcaacaggct ctttttgtat tgcgtttatt aagaaaaaca aaaaacatcg
     4681 atatacattt aactaaaaat tattgtatgt tatctccctt aattaggaat gataagggaa
     4741 taactagaaa gatttgtgaa tacaaactat ttctgatata caaaatatac agtaataatg
     4801 aatgatggga gatgggatga aagaatttca atttgagaga aagcagcgtt tttctttgag
     4861 gccatatgca ataggagctt gttcggtctt gctaggaacg agtttatttt ttgctggtat
     4921 gggtgctcag cctgtacagg atacagaaac gagttcagca ctaatttcaa gtcattattt
     4981 ggatgagcag gatttatctg aaaagctgaa atctgagttg caatggtttg aattagaaaa
     5041 caagcttttg aacttatggg agcattaggt tactatgaag gatttgttcc ttatgtttca
     5101 aatcaataca aaaaccaagc tgaagaagaa ggcaaaccgc tatctgataa atatattttc
     5161 gaaaaaatct taggaaaaac atatgcagcc ttcaaaaaag atcaaattaa tgagcgtgtc
     5221 gagaaattag gtaagttaaa acctattaca ataaattaca acggaaaatc agaagtaatt
     5281 gatagtaaag aaaaattaca agagcttatg aataaagccg ttaaagacga agtggctcaa
     5341 atatagtaaa atgaaataag aacaggataa atcgatcagg acagtcaaat cgatttctaa
     5401 aaatgtttta gaagtagagg tgtactattc tagtttcaat atactataaa atcaggcaat
     5461 accacggcta aaaagtttga gtttatcgaa acaccagtgc aaaaactgaa aaaagcgatt
     5521 tataaagctc atctcaaaga ttcagat
//
LOCUS       DAVLXF010000105         5400 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLXF010000105 DAVLXF010000000
VERSION     DAVLXF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.105"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            39..227
                     /locus_tag="VYO12_001895"
     CDS             39..227
                     /locus_tag="VYO12_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1588437.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            288..1046
                     /locus_tag="VYO12_001896"
     CDS             288..1046
                     /locus_tag="VYO12_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1588438.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDVRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            1104..1958
                     /locus_tag="VYO12_001897"
     CDS             1104..1958
                     /locus_tag="VYO12_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1588439.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEATYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            2070..2954
                     /locus_tag="VYO12_001898"
     CDS             2070..2954
                     /locus_tag="VYO12_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1588440.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3117..3890
                     /locus_tag="VYO12_001899"
     CDS             3117..3890
                     /locus_tag="VYO12_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEV1588441.1"
                     /translation="MELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDGVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQ"
     gene            3957..4856
                     /locus_tag="VYO12_001900"
     CDS             3957..4856
                     /locus_tag="VYO12_001900"
                     /inference="COORDINATES: protein motif:HMM:NF012356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1588442.1"
                     /translation="MTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYW
                     IEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAV
                     MNYPLSDSIKDYFLRGIKNTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTA
                     NEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFM
                     KRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAV
                     VLASNCQELENQLVISPDGFVIF"
     gene            5131..>5400
                     /locus_tag="VYO12_001901"
     CDS             5131..>5400
                     /locus_tag="VYO12_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588443.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFE"
BASE COUNT         1795 a          808 c         1148 g         1649 t
ORIGIN      
        1 tgttacacct gtgatagaat taacttgagg tgatgaaaat ggcaaccgat aaaaatagaa
       61 taatgattag tttggatgat aaaaatctag agaaacttga aaatttagta gaagatgcta
      121 gagatagaag gggaatgcga ctaacaaagt cccaggttat tgaattactc ctaaataccg
      181 ttgattattt cgatgatatt atgggggcta tttactcaaa aaaataagag caaaattgct
      241 attattttta caaattggtg taacactagt gatataggag tgatgaaatg gttgtttata
      301 ttagacaaag taaattacca agtgaggttt caattaataa atacaatgct caagtaggtg
      361 cctacttaca aggagaagaa gttattttat atcaatcttt ttctgaaata aaagagttaa
      421 caagtgaaga tatagttgta gattatataa tggagactag agcattacta aaaatgatgg
      481 gcttaaacgt tccggtttat gactatccta ttgagcttaa agagttttat ggtcgaaaga
      541 tttatgctgg tattttagga gagattgtga atatacctga taattgggga aaatttatta
      601 agcctaaagc tggttcaaaa gtctttactg gaagagttgt taatggaacc catgatttaa
      661 taggtattgg tctacctttc gactatccta tatggattag tgaggttgta gaattcatag
      721 ctgaatggcg ttgttttgtg ttagatgttc gcgtattaga tgttcgaccc tatacaggtg
      781 attatcatgc acaatttgat gcaagtgtaa ttgatgaagc gatatcatgt tggaaagatg
      841 cgccaatagc ttatggacta gatatcggtg ttactcgcga tggcagaaca cttgttgttg
      901 aagtaaatga tggttatgca ttgggaaatt atggcctatc tcctttaaaa tcaatcaatt
      961 ttcatagagc taggtggaaa gaaatggtaa aaccctattt tgaaaaaaat gaaattttta
     1021 aaatccaaca ggatgttatt ttctaaaata aatatggtag ataaccaata taaaaagcat
     1081 caaaatgaac aaggaggaat ctgatgcttg gagcaattat tggagatatt gtcggttctg
     1141 tttacgaatg gaacaatatc aaaacaaagg actttccttt atttcggaag gactgctttt
     1201 ttacagatga cacggttatg acctgtgctg ttgcagaagc aattatgaat ggtggacaaa
     1261 aagacgattt cattgacgcg atgaagaaat atggcagaat gtatccgaat gctgattacg
     1321 gtgctcggtt taatgcatgg ctaaacagcg ataaccgtga accttataat agctttggga
     1381 atggatcagc tatgcgtatt tctccatgtg cttggatcat ggactgtggt ttttatgcga
     1441 aaactggtat gtggccatca tctagaggac ttacgagtct ttctgcagag gtaactcata
     1501 atcatccaga aggtgtcaaa ggcgctatgg ctacagctga tgctatcttt ctatgtcgtt
     1561 tttactttgg tggttattgt agagagtacg agcaatcaat taacgacaat cctacagagt
     1621 gtaaaagacg aattaaggat tatatagaaa aagaatacga ttacaatcta tctcaaactt
     1681 tagatgaaat ccgtcctaac tatcgtttta acgaaacatg tcaggaaact gtccctcaag
     1741 ccattatcgc ctttcttgaa agtagagact tcgaagatgc gataagaaat gccatctcac
     1801 ttggtggcga cagtgatact ctcgcagcaa tcacttgtag catagcagag gcaacttacg
     1861 gtattcctga ttggatcaag gataaggcct attcttactt ggatgaaccc ttaaaggatg
     1921 tagttaggcg atgggaaaat agaataaaag cgtattaatc tccagtacgt cagatttctc
     1981 ttttctgtat tttcattgta taataggatt acatttactt gcatacgacg aaatgtaaac
     2041 atttctgttt aaaattaagg ataaaatcaa tgaaaaaagc aatggtaatt atcaacccta
     2101 cttctggtgg cgagaaggct ttggattaca aagaaaagct ggagaataaa gcaaaagaat
     2161 actttgaata tgttgaaacc aaaattaccg aaaaagcgct ggatgcaaca cattttgctg
     2221 aagaagcttc tcgtgagcag tatgatgcag tggttgtgtt tggtggagat ggaactgtca
     2281 atgaagtcat ttcaggtatt gatgagagag actacattcc taagttaggg attatcccag
     2341 gcggtacggg taacctcatt acaaaacttt tggaaatcaa tcaagacatc gatggcgcaa
     2401 ttgacgaact ggattttgat ttaaccaata agattgatat cggtaaagca aatgacaact
     2461 attttggtta tatctttagt atcggttctc tgcctgaggc gattcacaat gttgaaatag
     2521 aggacaaaac aaaattcggt attttaacct atgctgtaaa taccatgaag tctgtcatga
     2581 cagatcaggt ctttaacatt aaggttgaga cagaaaatgg aaattatgtt ggtgaagcta
     2641 gccatgtttt ggtccttttg acaaattact tcgctgataa gaaaatcttt gaagaaaaca
     2701 aggacggcta tgccaacatt ttgattctga aagatgcctc tatattctcc aaattatccg
     2761 tcattcctga tttattaaaa ggggatgtta tcgcaaatga taatatcgag tatatcaaag
     2821 cgcgtaatat taaaatctct tcagatagtg aattggagtc agatgttgac ggagataaat
     2881 cagataacct acctgtagaa atcaaagtcc tagctcagcg agtagaagta ttttcaaaac
     2941 cgaaagagga ttagtatata gagaaagcct tttttaaggc tttttgtata ctttaaaaga
     3001 tagttccttt aacaacggac attccttgca aatagtttac aaaaatagta tactggattt
     3061 attgagtttg aaaacgtttg cgtaaaattt gaatgaatac tttaggagac aaattgatgg
     3121 aattaagtgc tatttaccat gggcctgagt cggagtatgc ctatctttat aaggataaga
     3181 aactccatat tcgaattcga actaagaaag gggacattga aagcatcaac ttgcactatg
     3241 gggacccttt tatctttatg gaggagtttt atcaggatac aaaagaaatg gtcaagataa
     3301 cttctggtac cttatttgac cattggcagg ttgaagtgtc agttgacttt gcacgtatcc
     3361 agtatctctt tgagctcaga gatacagaag gtcaaaatat tttgtatggc gataaagggt
     3421 gtgtggaaaa ttctctagaa aatcttcatg caattgggaa tggatttaag ttgccttatc
     3481 ttcatgagat tgatgcctgc aaggttcctg acggggtttc aaatacggta tggtatcaga
     3541 tatttcctga aagatttgcc aatggcaatg ctctattaaa cccagaaggg actttagact
     3601 gggattcatc tgtcacacct aagagtgatg atttctttgg tggtgattta caggggatta
     3661 ttgatcatat ggattacttg caagacttgg gtattactgg actatatctt tgtcccatct
     3721 ttgaatctac aagcaatcac aagtacaata cgacagatta ctttgaaatt gaccgtcatt
     3781 ttggagacaa ggagaccttt cgggaactgg tggatcaagc gcatcatcgt ggcatgaaag
     3841 tcatgctgga tgcggtattt aatcatattg gttcgcaatc tcttcaatga aaaaatgtcg
     3901 tcaaaaatgg tgaacagtct gcttataagg attggttcca tattcaacaa ttcccagtga
     3961 caactgaaaa gctagttaat aagagagact taccctatca tgtttttggt ttcgaggact
     4021 atatgcctaa gctaaataca gccaatccag aggtcaagaa ttatctttta aaggttgcga
     4081 cttattggat tgaagagttt aatatcgatg cttggcgttt ggatgtggct aatgagattg
     4141 accatcagtt ctggaaggat tttcgtaagg cagttttagc taaaaatcct gatctttata
     4201 tcctaggaga agtctggcat acatctcagc cttggctaaa tggagatgag ttccatgccg
     4261 tcatgaatta tcctttatct gatagtatca aggactattt cttacgagga attaagaata
     4321 cagaccagtt catcgatgaa atcaatggag agtctatgta ttacaagcag cagatttcag
     4381 aggtcatgtt taatctcttg gattcacatg atacagagcg aatcctgtgg acggccaatg
     4441 aagatgttca actggttaaa tcagccttag cctttctctt tttacaaaaa ggaacaccgt
     4501 gcatttatta cggaaccgag ctagccttga ctggaggacc agatccagat tgtcgtcgtt
     4561 gtatgccttg ggaacgtgta tcaagtgaca atgatatgct gaactttatg aagaggctga
     4621 ttaagattcg gaaatacgcg tcagtaatca tttcgcatgg caagtatagc cttcaagaaa
     4681 tcaaatctga tctagtagct ctggaatgga aatacgaagg acggatcctc aaagcaatat
     4741 tcaaccaatc aacagaagat tatcttttag agaaagaagc agtagtacta gcaagcaatt
     4801 gccaagaatt ggagaatcag cttgtcatct ctccagatgg atttgtgatt ttctaaaaac
     4861 tagttgatga agattatggt acatttcata tcttatatag tataataagg ctagttacta
     4921 aacttgtaaa ggagaactta aatgaattgt agaggacatg aaacaagaca aagaattgtt
     4981 agagattttg aagtttagcc taaagcacat attaagctgt tagcaaatca acaaaaacat
     5041 agtgatgcag gagcaactat tgaagatgaa tattatgtat ttatcgctga gagtaaaatt
     5101 gatggcaaga aggaagttat tcagtgttgc atgggtgcgg caagggattt tttagaacta
     5161 attaatcaca aagggctacc tctttttaat ccgcttgtag gtgattctca tgtaaataat
     5221 agacaagaat atgacaatac agggagtgga aatttacaac ctgaaaagtg gaatgaaact
     5281 gcaaagcagc tttataatgt tataatgtgg ttgattattt tatggaatgc taagccggat
     5341 acacctttat ttaattttaa agacgaagta attaagtata aaacatatga gccttttgaa
//
LOCUS       DAVLXF010000106         5322 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLXF010000106 DAVLXF010000000
VERSION     DAVLXF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.106"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            243..695
                     /locus_tag="VYO12_001902"
     CDS             243..695
                     /locus_tag="VYO12_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588444.1"
                     /translation="MIYLLSNFLYAISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIV
                     AIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMMMNTSNDLFI
                     KAIELLPFYSLPVFVTSNTMSISQYLVVITTIILLYFFTLKKSKKYSF"
     gene            1081..1290
                     /locus_tag="VYO12_001903"
     CDS             1081..1290
                     /locus_tag="VYO12_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588445.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            1280..1768
                     /locus_tag="VYO12_001904"
     CDS             1280..1768
                     /locus_tag="VYO12_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588446.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            1884..3344
                     /locus_tag="VYO12_001905"
     CDS             1884..3344
                     /locus_tag="VYO12_001905"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1588447.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISYMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            3341..4165
                     /gene="nadE"
                     /locus_tag="VYO12_001906"
     CDS             3341..4165
                     /gene="nadE"
                     /locus_tag="VYO12_001906"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1588448.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            4462..5013
                     /locus_tag="VYO12_001907"
     CDS             4462..5013
                     /locus_tag="VYO12_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1588449.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1585 a          956 c         1158 g         1623 t
ORIGIN      
        1 atataaggga tttatattca tcaggcgtaa tatatctcgg tttgattatc tcgtttatac
       61 cgccgatatt gcttacattc tttattctaa agactcaagg gacatcgctt ggtattaagc
      121 atatttcaaa cttttatgct atgctcggta tgttaatggc tgttatacat gctaaccgaa
      181 ttattagcag ggatttttcc cacaatacga tcagtttatt ttataatcaa cagaaaaatc
      241 ggatgattta cctcttgtct aattttctat atgccatctc agtttccatt atttatgctt
      301 tgaatggcat tgtgttacta gtcatagtaa gtaaattggg tattccaggt gatttaggat
      361 tagattttat agtagctatt gtagtcaata caattttgtt agtcctgttt tattttctat
      421 tatcttacat tttctattta tacaaattga aaagtggctt ggtatttggt attttagtag
      481 ctttactact ctttatccct aatatattaa atacgatgat gatgaatact agtaatgatt
      541 tgtttatcaa agcaattgaa cttcttcctt tttattcttt acctgtattt gtgacttcaa
      601 atacgatgtc tattagtcag tatcttgtgg taatcactac aatcatttta ttgtactttt
      661 tcactctcaa gaaaagcaag aagtattcat tttagtttca tttagtatta ttttgcatac
      721 ctaaaataca ataaaaaaat cagtcatctt ggtatgctcc tgctttcact attcaacacg
      781 tttttgactt atactaggct catttccaaa agcattatat aatagtgata tgaaaccaac
      841 taaactaaac aagaaatata agcaataaaa actcgtttaa aagattctac taaagctaat
      901 actaaataaa aataaaagag taaactagga agtttatttc aaacaaccta aaatactgat
      961 tttcggctga agataatact ggagtgcaaa ttaatggggt tataataaat agctgatagc
     1021 ttgtgttggt tttggatttt ttaagagtag atgagtatta aaactataag gaggacgaag
     1081 gtggctaaaa atttaaaatt aaaattagct cgggtagagc gtgatttaac acaaggtcaa
     1141 ctggcagagg ctgtcggggt gacacgccag actattggtt tgatagaggc gggaaaatac
     1201 aatcccagtc tctcgctctg ccagtctatt tgcagatgtt tagggaaaac cctagaccaa
     1261 ctattttggg aggaagaaga tgaaaaatag attttattat tatcaattac tagacgaaag
     1321 agaagaacaa ctgttcaata aagcgggctc tgaaagtttc tatatctgca ttgctttgtc
     1381 gctcctatct tatatcattt cagtattagc accaagcctt tttaattcta atatgctgct
     1441 aatcgttatc atcataggga cattttactt tttcaatcgt gcccgttatc tgggagtgac
     1501 ctactatagt cgttttcatt ttacgatttt gggttgtttt ttcctaacct tggctattac
     1561 ggctcttttg atgttgcaga attatcaatt caacatagaa atttatcagc acaatccttt
     1621 gaattttaaa tacctgtctg cttgggtcat tacttatgtc atttaccttc cgtgggtctt
     1681 tattggcaat cttggtctta agagctatgg cgaatgggct cagaaaaaat ttgaacaaga
     1741 tatggatgaa ttggagagtg gagaatagct tgttactctt ttctcaatcc agctaaaatg
     1801 tgatataata gtattaattt attggaatac atgaaagttc ttgaaaattt tcatgggttt
     1861 ctagctaagg aagtaggaaa agtatgtatc cagatgatag tttgacattg cacacggact
     1921 tgtaccagat caacatgatg caggtttact ttgaccaagg gattcacaat aagaaggcgg
     1981 tctttgaggt gtatttccgc caacagcctt ttaagaacgg ctatgcggtt tttgcaggtt
     2041 tggaaagaat tgtgaactat cttgaagact tgcgtttttc agatagtgat atagcctatt
     2101 tggagtcgct tggttatcat ggggcgttct tggattacct tcgcaatttc aagttggagt
     2161 tgaccgttcg ttctgcccaa gaaggggatt tggtttttgc taatgaaccg attgtgcagg
     2221 tggaaggacc tctagcccaa tgtcagttgg tcgaaacggc tcttttgaac atcgtcaact
     2281 accagacctt ggtggcgacg aaggcagctc gtattcgttc ggttatcgaa gatgaaccct
     2341 tgatggagtt tgggacacgt cgggctcaag aaatggatgc ggccatctgg ggaacacgcg
     2401 cagctgtgat tggtggcgcc aatggaacca gcaacgtgcg tgcgggtaag ctctttgaca
     2461 ttcctgtttt gggaacccat gcccatgcct tggtacaggt ttatggaaat gactatgaag
     2521 ctttcaaggc ttacgctgcg acccacaaaa attgtgtctt tcttgtggat acctatgaca
     2581 cccttcgcat cggtgtacca gctgccattc aggtggcgcg tgagctgggt gatcagatta
     2641 actttatggg tgtgcggatt gactctgggg atattgccta catttctaag aaagtccgtc
     2701 agcaactgga cgaggctgga tttacagagg ctaagattta tgcttctaat gatttggacg
     2761 aaaatactat cctcaacctc aagatgcaaa aggccaagat tgatgtctgg ggcgtgggta
     2821 ccaagctgat tacagcatat gaccagccag ctcttggggc ggtttacaag attgttgcaa
     2881 tcgaagatga aactggtcag atgcgcaata cgattaagct gtctaataat gctgaaaaag
     2941 tctctacgcc aggtaagaag caggtgtggc gcattaccag tcgtgaaaaa ggtaagtcag
     3001 aaggtgacta catcacttat gatggtgtgg atattagcta catgacagaa atcaagatgt
     3061 tccatccgac ctatacatac atcaagaaga cggttcgtaa ttttgatgcc gttcctctct
     3121 tggtggatat cttcaaagaa ggaatattag tttacaactt gcctagtttg actgacattc
     3181 aggattatgc ccgtaaagaa tttgacaagt tgtgggatga gtataagcgt gtgctcaatc
     3241 cgcagcacta tccagtggat ttggcgcgtg atgtatggca agataagatg gacttgattg
     3301 ataagatgcg caaggaagcc cttggtgaag gagaagaaga atgagtttgc aagaaacgat
     3361 tatccaagag ctgggtgtca aaccagtgat tgatgcccag gaagaaatcc gtcgttctat
     3421 tgatttctta aaaagatatc tgaaaaaaca tcccttccta aaaacctttg tactagggat
     3481 ttctggggga caagactcaa ccttggcagg gcgtttggcg caattagcta tggaagaact
     3541 gcgagctgaa acgggagacg acagctacaa atttatcgct gtccgcctgc catacggagt
     3601 gcaagctgat gaagcagatg ctcaaaaagc cctagccttc atccagccag atgtcagctt
     3661 ggttgtgaat atcaaggaat cagctgatgc catgacagct gcagttgaag cgacaggtag
     3721 tcctgtttca gacttcaaca aggggaatat caaggcacgt tgccgtatga ttgctcagta
     3781 tgcccttgct ggttcccata gcggagcggt cattggaaca gaccacgccg cggaaaatat
     3841 cacaggtttc tttaccaagt ttggtgacgg cggtgcggat attctccctc tttaccgcct
     3901 caataaacgc caaggaaaac agctcttgca gaaacttggc gcagagccag ccctttatga
     3961 aaaaatccca acggcagacc tagaagaaga taaaccaggc ctagctgacg aagtcgcact
     4021 tggagtcacc tatgcagaga ttgacgatta cctagaaggc aaaacaatca gcccagaagc
     4081 tcaagcgacc attgaaaact ggtggcacaa aggccaacac aaacgccact tacccatcac
     4141 cgtatttgat gacttttggg agtaaaaacc tccattggag gtttttaccc tgagcctaga
     4201 aatgagaaag tgagagaaag tccgggggat cttgaacccc gagtttagaa ataagaaaat
     4261 gaggcagatt cggtaactcg aagagttcga tttcatcgtc ttacccctgc aacgatgact
     4321 aggtttgaaa aagcttgcta gatcgcattt caaaccaggc agcgactgcg tcaagaaatt
     4381 agaagacaaa ctcgttttct agctgttact gagttgagtc tttttactac gagcatagaa
     4441 ataaggaagt gaggtagcat catgaaatct atcggtacgc aaatattaca gacagaacgt
     4501 ttgattttaa gaagatttgt ggagagtgat gcagaagcca tgtttcaaaa ttgggcttca
     4561 tccgctgaga atttgaccta tgttacctgg gatccccatc ctgatgtcga aatcactcga
     4621 aactcgattc gcaattgggt tgcttcctat actaatctca actattacaa atgggccatt
     4681 tgtctaaaag aaaacccaga gcaagtgata ggagatatca gcatcgttaa gatagacgag
     4741 gctgatttaa gctgtgaaat cggctatgtg ctaggcaagg cttactgggg acatggtatg
     4801 atgacagagg ccttgaaagc tgtcttggac ttttgtttta ctcaagcagg ttttcaaaag
     4861 gtcagagcac gttatgccag tctcaaccca gcttcaggtc gtgtcatgga aaaggctgga
     4921 atgtcctatc tacaaaccat tgttaatggt gtagagagaa aaggctatct tgcggatctt
     4981 atttattatg gtataagtag ggaagaatgt tgaattctat tttctgtttc tatcgaagtc
     5041 aactatttat tgtaaatata ataattagca ttccaagttt atttgaaact ttaaaatagc
     5101 atattgatta gtacaagaca gatgttctag ttccttcttt aatctggttt agtattagtt
     5161 aaaaaatcgc ttgaagcttg taactaagag ggagctaatc gactaggttc tccagccgaa
     5221 caggtggtaa tgtacttttt atagtgtaat cctagctgtt gttaaattta aaatagaatc
     5281 ctctatcgag ttagggaatt aaattcaacc aattttattt at
//
LOCUS       DAVLXF010000107         5298 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLXF010000107 DAVLXF010000000
VERSION     DAVLXF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.107"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(19..783)
                     /locus_tag="VYO12_001908"
     CDS             complement(19..783)
                     /locus_tag="VYO12_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588450.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMVVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(879..1282)
                     /locus_tag="VYO12_001909"
                     /pseudo
     CDS             complement(879..1282)
                     /locus_tag="VYO12_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1462..2094)
                     /locus_tag="VYO12_001910"
     CDS             complement(1462..2094)
                     /locus_tag="VYO12_001910"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1588451.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2128..2829)
                     /gene="pyrF"
                     /locus_tag="VYO12_001911"
     CDS             complement(2128..2829)
                     /gene="pyrF"
                     /locus_tag="VYO12_001911"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1588452.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QLIKQATNPDFICLTPGIRPQGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            3150..3242
                     /locus_tag="VYO12_001912"
                     /pseudo
     CDS             3150..3242
                     /locus_tag="VYO12_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(3564..4277)
                     /locus_tag="VYO12_001913"
     CDS             complement(3564..4277)
                     /locus_tag="VYO12_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1588453.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFCKYLMETQG
                     KVNDQQELVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(4270..5109)
                     /locus_tag="VYO12_001914"
     CDS             complement(4270..5109)
                     /locus_tag="VYO12_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588454.1"
                     /translation="MSMIEVSHLSKSFGDKIALNNISFTVKEGQIFGFLGPSGSGKTT
                     TINILTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSK
                     FYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSG
                     LDPTTSRTIHELILELKTAGTTIFLTTHDMNEASLLCDYVALLNKGKLVEQGAPSELI
                     QRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGK
                     LNA"
BASE COUNT         1750 a         1066 c          868 g         1614 t
ORIGIN      
        1 attgaacaaa ttgtttcatt atacatagtc ctctgaatgt agaaaaaatg taccataaac
       61 aaccaaacaa ctaacaaata aaataaaagc aagatgccca ctaactaagg aaagactgat
      121 atctttctga tatcccaaag ctagcgttgt cacaggttct aagtaagata gccctaaaat
      181 agcccataaa ataccaccaa ccaccatata ggcaactggg atgaaaatag ctcctaattt
      241 tttcttcact agcaaagcac tagctagtcc aaaaatagag aacacagcgc cccaaactcc
      301 ataccagaga gtcgtcacaa gactatagag aaactgatta gaatcaaata attcttttaa
      361 ggcaccacta taatctccaa tataaatttc ctgataagga gtcactaaaa gattaattcc
      421 taataataat aaatagggga gaaaaaagac tagaaaagaa gaaataattg cagtactacc
      481 tacaatagct agatacctct ttttagaaat tcggcacaat tgtgctgtta gaaaatgact
      541 ctcagcatcc tctattatct gactagaata gggcagtgta cagataagtg cagctactag
      601 gctaatcggt gaaaatcctc gaatagaaga agcggcaaaa aaaatcgata aaccttcaat
      661 tttataaata ccattgaaag caaggaaatt tcctaaactc atgcaaagaa gggtcaaaaa
      721 taaagacata taaaaccgag gtgattgaac gactcgtaca agattacaaa tgaaaaattc
      781 catccttact cctccttata ataaaaatag ggtgtagcat tcttttttca tgctacaccc
      841 acaatcaacc atctttaagg cttactctga caagtaagtt aataagaatc tggactccaa
      901 gaacctgaag tatgaattct tacatgattt ccaaattgtg gcgccatagc taatctagta
      961 ccagaaccaa tataattgtc accacctcca ttatagtaca tgacaatcct agagccagac
     1021 cccaatgaat ataccggggt aatatctgac ccactatagg cgctacgaat agaggtattt
     1081 aaccttttac cgccaccagt gctgtcacta ttattaattc cagcagaggc gttttctttc
     1141 tcaagctcac tagttgtatc cgaaccccaa cgcggcaacg ttaaatcata atttccaatc
     1201 tgtctagcta aaactgtagg gagaaacaaa ctagatgcaa ttgtaactac gcaaaaaagg
     1261 aatatatttt tcttcttttc atgacatcgt cctcttaaga agtgataaac aagatagttg
     1321 attgatcaat ttgatacata ttacattttc attatatcaa taaaagaaag cgttgtcaat
     1381 tattttattt aaaatattat catattttaa ataaaatggt aaaagagact cggtagcctc
     1441 gtctctcttt atcttactga cctaaccttc ttgccaattt tcttggtctt ctttaaagcg
     1501 ttttagaaga tcaaggccct ctggcgtgat gtaaccttct tcttgggcta gatggataag
     1561 ctcgctatag tttgaaagcg tcacaagttt aacaccagca tctgcaaagt tcttatctgc
     1621 ttttggcaat tggtagctga aaatcgctac aactccaagt acatctgctc cttctcgctt
     1681 ggctgctgct acagcttcaa gaactgaacc acccgttgaa ataaggtctt caaccactac
     1741 cattttttga ccttgagcta cgcgaccttc gatttgatta ccagctccgt ggtcttttgg
     1801 tttgctacgg atgtaggcaa aaggcaagtc catcttatca gcaataatgg ctccgtgtgg
     1861 aatccctgct gttgcagttc ctgcaatcac ttctacttca ggaaaggctt ctttgatagc
     1921 ttccacaaaa ccattttcaa ttagggtacg agtttctgga taggctagtg tcacacgatt
     1981 atcagtgtaa atcggtgact tgataccaga cgcccaagtg aagggttcct ctggtttgag
     2041 gtaaacggct tggattttca agaggtggct agcgatatct ttagcaagtg tcatggtatt
     2101 ctccttttat ttttctaatc tagttcttta attccagtcc tgtgtccatt catccttgat
     2161 ggcatgataa gctgcaacag gatcctcagc ttgggtaatg ggacgtccca ctacgatata
     2221 gtcactacca atttgataag catctgctgg cgtcatgacg cgtttttggt ctccaacagc
     2281 agctccctgt gggcgaatcc ctggtgtcag acagataaaa tctggattgg tggcctgctt
     2341 gataagttga acttcctgtg ccgagcaaac gacaccatcc aagcctgctt cagctgtctt
     2401 cttggcatag tggatcacag actcttgtag gcttgtttgg atattttgaa actcctgcat
     2461 ctgggcttct gatgttgatg tcagctgcgt tacagcgatc aattttgctt gacttccaag
     2521 accttcacgc gcagccttca tcatctctac accaccagcc gcatgaacat tggtcatatc
     2581 gacaccaagc tgagacaaga ccttcatggc tgacttgact gtattaggaa tgtcatgaag
     2641 tttgagatcc aaaaagacac tatgacccaa accttttaag taggacacaa tctcaggccc
     2701 cgctgcgtaa taaagctcca tccctacctt gagataaagg ctttcttctg ctgggaaaag
     2761 agctaaaaat tccttgaccg cctcaaaact aggaaaatca agagcaatga ttggacgatg
     2821 ttctcgcata ggtttctcct tttaccttta ctagtgagag agtctggcca acagaagcca
     2881 cgaaaaaagg aacctgccaa cagcaaggtt ccacgaaaaa tgggtagtct ccaccctttc
     2941 accgtctaac cttagctgcc tctctggact gctttaaagg ttttctatta ttctattatt
     3001 tctactagca cttgtcaagt aaaaacatgg tcacgtatgt gttgactgct attgaacacc
     3061 tcacaagact ctttccaaaa gaatagttaa agactttcac tgaggacaga gaaaaagagt
     3121 ttgcttgtta ccctttaatt gaacaaatgg gaattctgtt ttattttgcg gatgcctatt
     3181 catcttggca aaggggaagt aatgagaatg cgaatggact tcttcgtgag tattttctta
     3241 aaccgatttg gcaacaatta ccgaaaaaat ccttaatggt gctttgctag cttattaatc
     3301 accgttccca aaaatacttg gattacaaga caggttttga agcacttata gaggagattt
     3361 aaaaatgttg cattttttat tgcaatttat cagttatctt atcacccaaa acttctgcaa
     3421 ttagtatgag cactattgtt atttttcttt tttcgtacac tcaaatcaaa atatagcaat
     3481 aagactattc tgtagaattg ctgtcaaaaa gatcagttca tttttccttc ccacttgtgt
     3541 ttataatcat taaaataact caattaagaa attttctttt tcctaatagt tatcgtaatt
     3601 aaagttaata gaagaacaat ccaaataagt agtgttagat taggaattag agtttcattc
     3661 catgaaaatc cctcccattt tgtgaaaaac ttagtaaaca gtcccataaa actataatct
     3721 gttatcttcg caactgtctt atccaaacca gatagcatcg gtaagaaagc aactaaaatc
     3781 atagcaggaa gactgataac ctgagctact acttggctct tcgcggttaa acctatcaat
     3841 aaatagaata aaatgatgga taaagaggtt agaagaacga ttgtaatata attaaaagta
     3901 tgtacaattg taactcctaa aataagagga gtagttccca taatcacaaa agttagcaaa
     3961 aaaggaagaa acatcgttga taaaatgtat tcggatcctt taacaccact caacagaaga
     4021 gtttgtaaat tgtacttttc tttttcttca gacaagataa tagttatagg acttccaaca
     4081 gccaaagcca aagaaaaagg taaacacatc atcaaaagaa ctaattcctg ttgatcgttg
     4141 accttcccct gtgtttccat aagatattta caaaaatatg tgaaagcaaa aggcactaaa
     4201 acttgcaata aaatactctt attggaaagg ataatttgac aacgcaacca taccaaagcc
     4261 agaaaccgtt taagcattta gctttcctcc tgttaatgtg ataaaaatat cttctaaagt
     4321 aggctcacat gaatgaattg aaaaaatatt ttccagatca gcctgagata cctgttctag
     4381 tgatgtaaaa tcaaaagtta tctgattccc attataatct gtaaccttaa tctttttatc
     4441 tttattatat ctttgaatga gttcagaagg agctccttgc tcaactaatt tccctttatt
     4501 taataaggca acataatcac ataaaagact tgcttcattc atatcatgag tcgttagaaa
     4561 aatcgttgtc cctgctgttt tcaattctaa aattaactca tgaattgttc gagaagttgt
     4621 gggatctaga cctgaggtcg gttcatccag aaagagtaca gcgggtttgt tgataagagc
     4681 tcgtgctaaa agcattcgtt gcctcattcc agtggataat tttcctgcta ccatcttgcg
     4741 actatcatat aatcctactc gctttaacaa attatcaaca cgtgatttac taatattata
     4801 aaatttacta taaaaaagaa gattgttata cagagacatt ttctcataaa atccacttgt
     4861 atcgctaacc aatccaattc tctttaattc accgcttgtt aaattttgag atttttgtcc
     4921 caaaataata gattgtcctt tatcggcaag gaactgccca gtcagaatat taatcgttgt
     4981 ggtttttcca gaaccagatg gtcctaaaaa tccaaaaatc tgaccttctt taacagtgaa
     5041 gcttatatta tttaaagcta ttttatcacc aaaacttttt gataaatggc taacttcaat
     5101 catactcatt gcacctcctt tatattctac atttttactt aacatctatt tacttagctt
     5161 tatcaaataa acgtcttaaa aaaaacaata acattaagca tataaccgtt agcaagattg
     5221 tttcaatatt attgataata tgatacattc tcaagcagta taaaataatt atatatatgc
     5281 aaatgctcat tataccta
//
LOCUS       DAVLXF010000108         5289 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLXF010000108 DAVLXF010000000
VERSION     DAVLXF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.108"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            97..168
                     /locus_tag="VYO12_001915"
     tRNA            97..168
                     /locus_tag="VYO12_001915"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:Glu,seq:ttc)
     gene            422..1967
                     /locus_tag="VYO12_001916"
     rRNA            422..1967
                     /locus_tag="VYO12_001916"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2019..2091
                     /locus_tag="VYO12_001917"
     tRNA            2019..2091
                     /locus_tag="VYO12_001917"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2052..2054,aa:Ala,seq:tgc)
     gene            2216..5117
                     /locus_tag="VYO12_001918"
     rRNA            2216..5117
                     /locus_tag="VYO12_001918"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5194..>5289
                     /gene="rrf"
                     /locus_tag="VYO12_001919"
     rRNA            5194..>5289
                     /gene="rrf"
                     /locus_tag="VYO12_001919"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1519 a         1060 c         1536 g         1174 t
ORIGIN      
        1 tagtaagtca tgcaaaaaaa atgaaaaaaa ttagaaaaag tagttgacaa agtttgaaaa
       61 gactgtataa tagtaagagt tgaaaataac aactcaggtc cgttggtcaa ggggttaaga
      121 caccgccttt tcacggcggt aacacgggtt cgaatcccgt acggactatg gtatgttgcg
      181 gttggaacac ttgatgaaaa aaagtttaaa aaaagcttaa aaatcttcaa aaaagtgttg
      241 acaagtgaaa gtggctgtga tatactaata tagttgtcgc ttgagagaag caagtgacaa
      301 agacctttga aaactgaaca agacgaacca atgtgcaggg cgctacaaca taagttgtag
      361 tactgaacaa tgaaaaaaac aataaatctg tcagtgacag aaatgagtaa gaactcaaac
      421 ttttaatgag agtttgatcc tggctcagga cgaacgctgg cggcgtgcct aatacatgca
      481 agtagaacgc tgaaggagga gcttgcttct ctggatgagt tgcgaacggg tgagtaacgc
      541 gtaggtaacc tgcctggtag cgggggataa ctattggaaa cgatagctaa taccgcataa
      601 gagtggatgt tgcatgacat ttgcttaaaa ggtgcacttg catcactacc agatggacct
      661 gcgttgtatt agctagttgg tggggtaacg gctcaccaag gcgacgatac atagccgacc
      721 tgagagggtg atcggccaca ctgggactga gacacggccc agactcctac gggaggcagc
      781 agtagggaat cttcggcaat ggacggaagt ctgaccgagc aacgccgcgt gagtgaagaa
      841 ggttttcgga tcgtaaagct ctgttgtaag agaagaacga gtgtgagagt ggaaagttca
      901 cactgtgacg gtatcttacc agaaagggac ggctaactac gtgccagcag ccgcggtaat
      961 acgtaggtcc cgagcgttgt ccggatttat tgggcgtaaa gcgagcgcag gcggttagat
     1021 aagtctgaag ttaaaggctg tggcttaacc atagtaggct ttggaaactg tttaacttga
     1081 gtgcaagagg ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga
     1141 acaccggtgg cgaaagcggc tctctggctt gtaactgacg ctgaggctcg aaagcgtggg
     1201 gagcaaacag gattagatac cctggtagtc cacgctgtaa acgatgagtg ctaggtgtta
     1261 gaccctttcc ggggtttagt gccgtagcta acgcattaag cactccgcct ggggagtacg
     1321 accgcaaggt tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg
     1381 tttaattcga agcaacgcga agaaccttac caggtcttga catccctctg accgctctag
     1441 agatagagtt ttccttcggg acagaggtga caggtggtgc atggttgtcg tcagctcgtg
     1501 tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc ctattgttag ttgccatcat
     1561 tcagttgggc actctagcga gactgccggt aataaaccgg aggaaggtgg ggatgacgtc
     1621 aaatcatcat gccccttatg acctgggcta cacacgtgct acaatggctg gtacaacgag
     1681 tcgcaagccg gtgacggcaa gctaatctct taaagccagt ctcagttcgg attgtaggct
     1741 gcaactcgcc tacatgaagt cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa
     1801 tacgttcccg ggccttgtac acaccgcccg tcacaccacg agagtttgta acacccgaag
     1861 tcggtgaggt aaccgtaagg agccagccgc ctaaggtggg atagatgatt ggggtgaagt
     1921 cgtaacaagg tagccgtatc ggaaggtgcg gctggatcac ctcctttcta aggataagga
     1981 actgcgcatt ggtcttgttt agtcttgaga ggtcttgtgg ggccttagct cagctgggag
     2041 agcgcctgct ttgcacgcag gaggtcagcg gttcgatccc gctaggctcc attggtgaga
     2101 gatcaccaag taatgcacat tgaaaattga atatctatat caaatagtaa caagaaaata
     2161 aaccgaaaac gctgtagtat taataagagt ttatgactga aaggtcagaa aataaggtta
     2221 agttaataag ggcgcacggt ggatgccttg gcactaggag ccgacgaagg acgtgacaaa
     2281 cgacgatatg ccttgggtag ctgtaagtaa gcgatgatcc agggatttcc gaatggggga
     2341 acccaacagg taatacctgt tacccacatc tgttaaggat gtgaggagga agacgcagtg
     2401 aactgaaaca tctaagtagc tgcaggaaga gaaagcaaaa gcgattgcct tagtagcggc
     2461 gagcgaaacg gcagaagggc aaaccgaaga gtttactctt cggggttgta ggactgcaat
     2521 gtggactcaa agattataga agaatgattt gggaagatca gccaaagaga gtaatagcct
     2581 cgtatttaaa atagtctttg tacctagcag tatcctgagt acggcgggac acgtgaaatc
     2641 ccgtcggaat ctgggaggac catctcccaa ccctaaatac tccctagtga ccgatagtga
     2701 accagtaccg tgagggaaag gtgaaaagca ccccgggagg ggagtgaaat agaacctgaa
     2761 accgtgtgcc tacaacaagt tcgagcccgt taatgggtga gagcgtgcct tttgtagaat
     2821 gaaccggcga gttacgttat gatgcgaggt taagttgaag agacggagcc gtagggaaac
     2881 cgagtctgaa tagggcgcct tagtatcatg acgtagaccc gaaaccatgt gacctaccca
     2941 tgagcaggtt gaaggtgcgg taagacgcac tggaggaccg aaccagggca cgttgaaaag
     3001 tgcttggatg acttgtgggt agcggagaaa ttccaaacga acttggagat agctggttct
     3061 ctccgaaata gctttagggc tagcgtcgac attagagatt cttggaggta gagcactgtt
     3121 tgggtgaggg gtccatcccg gattaccaat ctcagataaa ctccgaatgc caatgaatta
     3181 tggtcggcag tcagactgcg agtgctaaga tccgtagtcg aaagggaaac agcccagacc
     3241 accagctaag gtcccaaaat aattgttaag tggaaaagga tgtggggttg cacagacaac
     3301 taggatgtta gcttagaagc agctattcat tcaaagagtg cgtaatagct cactagtcga
     3361 gtgaccctgc gccgaaaatg taccggggct aaaacaattt accgaagctg tggatacctt
     3421 tataggtatg gtaggagagc gttctatgtg tgatgaaggt ataccgtgag gagtgctgga
     3481 acgcatagaa gtgagaatgc cggtatgagt agcgaaagac aggtgagaat cctgtccacc
     3541 gtaagactaa ggtttccagg ggaaggctcg tccgccctgg gttagtcggg acctaaggag
     3601 agaccgaaag gtgtatccga tggacaacag gttgatattc ctgtactaga gtatgtagtg
     3661 atggagggac gcagtaggct aactaaagca gacgattgga agagtctgtc taagcagtga
     3721 ggtgtgaatt gagtcaaatg cttaattcta taacattgag ctgtgatggg gagcgaagtt
     3781 tagtagcgaa gttagtgacg tcacactgcc aagaaaagct tctagcgttt aaacatactc
     3841 tacccgtacc gcaaaccgac acaggtagtc gaggcgagta gcctcaggtg agcgagagaa
     3901 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgacttt
     3961 aagtcagccg cagtgaatag gcccaagcaa ctgtttatca aaaacacagc tctctgctaa
     4021 atcgtaagat gatgtatagg gggtgacgcc tgcccggtgc tggaaggtta agaggagtgc
     4081 ttagcgtaag cgaaggtatg aattgaagcc ccagtaaacg gcggccgtaa ctataacggt
     4141 cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg cgtaatgatt
     4201 tgggcactgt ctcaacgaga gactcggtga aattttagta cctgtgaaga tgcaggttac
     4261 ccgcgacagg acggaaagac cccatggagc tttactgcag tttgatattg agtgtctgta
     4321 ccacatgtac aggataggta ggagtctaag agatcgggac gccagtttcg aaggagacgc
     4381 tgttgggata ctacccttgt gttatggcca ctctaaccca gataggtgat ccctatcgga
     4441 gacagtgtct gacgggcagt ttgactgggg cggtcgcctc ctaaaaggta acggaggcgc
     4501 ccaaaggttc cctcagaatg gttggaaatc attcgcagag tgtaaaggta taagggagct
     4561 tgactgcgag agctacaact cgagcaggga cgaaagtcgg gcttagtgat ccggtggttc
     4621 cgtatggaag ggccatcgct caacggataa aagctaccct ggggataaca ggcttatctc
     4681 ccccaagagt tcacatcgac ggggaggttt ggcacctcga tgtcggctcg tcgcatcctg
     4741 gggctgtagt cggtcccaag ggttgggctg ttcgcccatt aaagcggcac gcgagctggg
     4801 ttcagaacgt cgtgagacag ttcggtccct atccgtcgcg ggcgtaggaa atttgagagg
     4861 atctgctcct agtacgagag gaccagagtg gacttaccgc tggtgtacca gttgtcttgc
     4921 caaaggcatc gctgggtagc tatgtaggga agggataaac gctgaaagca tctaagtgtg
     4981 aaacccacct caagatgaga tttcccatga ttatatatca gtaagagccc tgagagatga
     5041 tcaggtagat aggttagaag tggaagtgtg gcgacacatg tagcggacta atactaatag
     5101 ctcgaggact tatccaaagt aactgagaat atgaaagcga acggttttct taaattgaat
     5161 agatattcaa ttttgagtag gtattactca gagttaagtg acgatagcct aggagataca
     5221 cctgtaccca tgccgaacac agaagttaag ccctagaacg ccggaagtag ttgggggttg
     5281 ccccctgtg
//
LOCUS       DAVLXF010000109         5287 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLXF010000109 DAVLXF010000000
VERSION     DAVLXF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.109"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(286..1776)
                     /gene="lysS"
                     /locus_tag="VYO12_001920"
     CDS             complement(286..1776)
                     /gene="lysS"
                     /locus_tag="VYO12_001920"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1588455.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(1904..2050)
                     /locus_tag="VYO12_001921"
     CDS             complement(1904..2050)
                     /locus_tag="VYO12_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1588456.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            2083..3000
                     /locus_tag="VYO12_001922"
     CDS             2083..3000
                     /locus_tag="VYO12_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275026.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1588457.1"
                     /translation="MYIAVWGSLGSFLLGLIVSIIRHYRIPVLAQVATAYIELSRNTP
                     LLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSAMQDSGIQVLFQGNNLLRILQGL
                     GVTIGISILSVLLSMMFGTVMGIIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFG
                     LARNFNINISGETSAIIVFTLWGTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYY
                     HIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFW
                     IYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            3001..3759
                     /locus_tag="VYO12_001923"
     CDS             3001..3759
                     /locus_tag="VYO12_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1588458.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGASGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRTLLMHPE
                     IILFDEVTASLDSEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAHAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            3771..4643
                     /locus_tag="VYO12_001924"
     CDS             3771..4643
                     /locus_tag="VYO12_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1588459.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLARKTSSTRPMKKHFTQPTVTLLK
                     QMTWLLKVEKLISH"
     gene            complement(4723..5274)
                     /locus_tag="VYO12_001925"
     CDS             complement(4723..5274)
                     /locus_tag="VYO12_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588460.1"
                     /translation="MGITGANGSGKSVLFKLIAGLYSPSYGEVLINGENIVPERKIPA
                     NLGALIEEPGFINYYSGFKNLQYLASIRGVVGNQEINDTLKIVGLYEQKDQKVKTYSL
                     GMRKKLGIAQAIMENPSILLLDEPMNALDKSSVENMRTLFRKLSSEKGTTILIASHSE
                     EDIRILCDKVYAIEDKVCTLCSD"
BASE COUNT         1533 a         1131 c         1003 g         1620 t
ORIGIN      
        1 ctaacttcct atcccactta ttaatttttt atttatagtg aatctgaaca aatcaattgg
       61 ggaatccaag tcaatttcta gtactttttg aacagtttca gcgtactatc gtaaaatgaa
      121 ataaaacatg cacaaattgc ttagggaatt taaaccaatt tctaacaatg tcttagaaat
      181 caaagtgtac tatttcaact tcaatatact acacttcatt ttctccttaa attcagtctc
      241 tttttgaatc aaaaaatccc ctggtaagag ccagaggata agaatttatt tcattgttgg
      301 gaagagcaat acatcacgga tagttgttgt atcagtgagg agcatgcaga gacggtcgat
      361 accgattccc aaaccacctg ttggtggcat accgtattca agagcttcaa tgtagtcata
      421 gtcgattcct gtcgcttcat catcaccaag ttctttggct ttagcttggg cttcaaaacg
      481 gctaagttgg tcgattgggt cgttcaactc agtaaaggca ttaccgtact ccttagtcat
      541 gataaagagc tcgaaacggt cagtaaagcg ttggtcttca ggatttttct tagcgagtgg
      601 agatacagct actggatgtc catagacaaa ggttggttgg attaaagttt cttcaacaaa
      661 ctcttcaaag aaggcattga tgatgtgacc aacctcagtg tagtgtttct caactggaac
      721 tttcttctca gcagcgatag ttttagcttc ttccaaagtc atgtcttgcc agaaatcgac
      781 accagtaatt tctctgatag catccaccat atgaacacgc ttaaatggtt cgttaatctt
      841 gatttcagta ccttggtagt tgactgggcc atcgcctttg actgatttag cagcatgttg
      901 gataatgcct tccgtcaagt ccatgatatc ttggaagtct gcataagctt ggtaaacttc
      961 gatagaagtg aactcagggt tatgagtagc gtccattcct tcgttacgga agatacggcc
     1021 aatttcatag acacgttcca taccacccac aataaggcgt tttaagtgaa gctcagtcgc
     1081 gatacgaagc accatgtcaa tgttttgggc attgtggtgg gtgataaatg gacgggcagc
     1141 agcaccaccg gcttcattat gaagaacagg tgtttccact tcaaggaatc ctttttggtc
     1201 aaggtaacga cggatttcag agatgatttt tgaacgagtg acaaagcgtt caaagctttc
     1261 acgattagaa atcaagtcaa ggtaacgttt acggtaaatt gtttcaacgt ctgtcaaacc
     1321 atggaatttc tcaggaagag gacgaagagc cttagacaag tgtgtgatgt gggttgcctt
     1381 gatagagagt tctcccatat ccgtacgcat cacttcacct tcgacaccaa ggaagtcacc
     1441 aaggtctgct tttttgaaga tttcgtagtt ttcttcaccg acagcatcct tacgaacata
     1501 gatctgaatc tggccttcgc ggtcttgaag gtgggcaaaa ccaacttttc ctttaccacg
     1561 tttggttacc aagcgtcctg cgatagtagc tgtttcgttt ttatcgtgta attgttcttt
     1621 atcgaggttg gcatatttat cttttaattc ttgtgaattt gcagtacgtt caaaacgttt
     1681 tccgaaagga tcgattcctt gttcgcggag cgcagccatt ttttcacggc gaacgatctg
     1741 ctggtcattt agttcttcca tatgttctgt agacatggga tcctcctagt tttactcaaa
     1801 attgaatacg atgagtcatt ttttgcgata ctcccatttt atcataaata agcaagaaat
     1861 tcacggataa tctttaaaaa ctaaaaagaa cctgacgcta aaatcactgg aaagaagtga
     1921 aagtatcttc cgccccacta gcgatatagc caaatgctgc tttaggaata acttgttgcg
     1981 ccattggctc caaatcatag gtattgatga aatctacatg accttctgca ttgcttgttt
     2041 tgtatgacat aaaatgcact ccttgataag taagcgttta ctttgtatat tgcagtttgg
     2101 ggaagtttgg gatcctttct gctcggttta atcgttagta tcatccgaca ttatcgaatc
     2161 cctgttttgg cgcaagtagc gacagcctac attgaattgt cacgtaatac gccccttttg
     2221 attcaactct tctttctcta cttcggtctt ccccgaatcg ggattgtcct atcttcagaa
     2281 gtctgtgcaa cgcttgggct tgtcttttta ggaggctccg ccatgcagga ttcgggaatc
     2341 caagtactct ttcaaggaaa taatctcctg agaatcttac agggattggg cgttacgatt
     2401 gggatatcca tcctgtctgt cctcttatcc atgatgttcg gaacagtcat gggaatcatc
     2461 atgacctccc attctagaat catacgattt ttaacacgat tgtatctgga ctttacccgt
     2521 atcatgcccc agctggtgct actcttcatc gtttactttg gcttggctcg aaactttaat
     2581 atcaatatct caggtgagac ttcagctatt atcgttttta ccctctgggg aacagctgaa
     2641 atgggagact tggtacgtgg agctatcact tctctcccta aacatcagtt tgaaagtgga
     2701 caagcactcg gcttgactaa tgttcaactt tactaccaca tcatcatccc acaagtcttg
     2761 agaagactgc taccgcaggc tatcaatctt gtcactcgga tgattaaaac cacttcatta
     2821 gttgttttga ttggggttgt ggaagtgacc aaagttggac aacaaatcat cgatagcaat
     2881 cgcctgacca tcccaactgc ttcattttgg atttatggaa ccattctagt cttatatttc
     2941 gcagtttgct accctatttc caaactatcc actcacttag aaaaacattg gagaaactaa
     3001 atgtctgaaa ctatcttaga aatcaaggaa ctaaaaaaat ccttcggaga caatcccatc
     3061 ctccaaggac tttctctaga aattaaaaaa ggggaagttg ttgtcatcct aggggcatct
     3121 ggttgtggga aaagtaccct ccttcgttgc ctcaacggct tagaaagtat tcaaggtgga
     3181 gatatttttc tggatggtca gtctatcgtt gaaaataaaa aagattttca cctagttcgc
     3241 caaaagattg gcatggtctt tcaaagttat gaactctttc cccatctgga tgtcttacaa
     3301 aacctcatcc taggccctat caaaactcaa ggaagggaca agaaagaagt aacggaagaa
     3361 gctttgcaat tactagatcg tgtcggtttg ctggataaac aacatagctt tgcccgtcaa
     3421 ttatctggtg gacagaagca acgtgttgca attgtccgta ccctcctaat gcatccagaa
     3481 atcatccttt ttgacgaggt gactgcttcg ctggattcag aaatggtgcg tgaggtgctg
     3541 gaacttatca atgatttggc ccaagaaggc cgtaccatga ttttagtaac ccacgaaatg
     3601 cagtttgccc aagccattgc tgaccggatt atcttcctcg accaagggaa aatcgctgaa
     3661 gaaggaacag ctcatgcctt ctttaccaat ccgcaaacca aacgagccca ggaattttta
     3721 aacgtctttg actttagcca atttggctca tatctataaa ggagattctt atgaaactat
     3781 tcaaaccact cttaactgtt ttagcacttg cctttgccct tatctttatc actgcttgta
     3841 gctcaggtgg aaacgctggt tcatcctctg gaaaaacaac tgccaaagct cgcactatcg
     3901 atgaaatcaa aaaaagcggt gaactgcgaa tcgccgtgtt tggagataaa aaaccgtttg
     3961 gctacgttga caatgatggt tcttaccaag gctacgatat tgaactaggg aaccaactag
     4021 ctcaagacct tggtgtcaag gttaaataca tttcagtcga tgctgccaac cgtgcggaat
     4081 acttgatttc aaacaaggta gatattactc ttgctaactt tacagtaact gacgaacgta
     4141 agaaacaagt tgattttgcc cttccatata tgaaagtttc tctgggtgtc gtatcaccta
     4201 agactggtct cattacagac gtcaaacaac ttgaaggtaa aaccttaatt gtcacaaaag
     4261 gaacgactgc tgagacttat tttgaaaaga atcatccaga aatcaaactc caaaaatacg
     4321 accaatacag tgactcttac caagctcttc ttgacggacg tggagatgcc ttttcaactg
     4381 acaatacgga agttctagct tgggcgcttg aaaataaagg atttgaagta ggaattactt
     4441 ccctcggtga tcccgatacc attgcggcag cagttcaaaa aggcaaccaa gaattgctag
     4501 acttcatcaa taaagatatt gaaaaattag caaggaaaac ttcttccaca aggcctatga
     4561 aaaaacactt cacccaaccc acggtgacac tgctaaagca gatgacctgg ttgttgaagg
     4621 tggaaaagtt gattagtcat taactcttaa aaggaactgg attttaagct ccaatccctt
     4681 tttaagattt tacctataac atcctgagtc tatctaagat gttcaatctg aacacagtgt
     4741 acatacttta tcttctattg catatacttt atcacataag atacgaatat cctcttcact
     4801 atgactagca atcaaaattg ttgtcccttt ttcactagag agctttctaa acaatgttct
     4861 catattttct acacttgatt tatccaaggc attcataggt tcatctagta aaagaataga
     4921 gggattctcc ataattgctt gagcaatccc tagctttttc ctcataccta gcgaataagt
     4981 tttaactttc tggtcttttt gctcatatag accaactatt ttcagtgtat cattgatttc
     5041 ctgattacca actactcctc gtatgcttgc caaatattgt aaattcttaa agccactata
     5101 ataatttata aaaccaggtt cttcaatcaa agctcccaaa ttagctggaa tttttctctc
     5161 aggaacaata ttttccccat tgattaacac ttctccataa gacggactat ataaaccagc
     5221 tattaattta aacaatacac ttttacctga gccattcgca ccagtaattc ctataatttc
     5281 cccctgt
//
LOCUS       DAVLXF010000110         5216 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLXF010000110 DAVLXF010000000
VERSION     DAVLXF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.110"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(364..1092)
                     /locus_tag="VYO12_001926"
     CDS             complement(364..1092)
                     /locus_tag="VYO12_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1588461.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1250..2659
                     /locus_tag="VYO12_001927"
     CDS             1250..2659
                     /locus_tag="VYO12_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1588462.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2677..3972
                     /locus_tag="VYO12_001928"
     CDS             2677..3972
                     /locus_tag="VYO12_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1588463.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLALVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3977..4285
                     /locus_tag="VYO12_001929"
     CDS             3977..4285
                     /locus_tag="VYO12_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1588464.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4282..4590
                     /locus_tag="VYO12_001930"
     CDS             4282..4590
                     /locus_tag="VYO12_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1588465.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1758 a          879 c          948 g         1631 t
ORIGIN      
        1 aaattgggag attgaaattc atttctagca attttttaga agtcacagtc tactattcta
       61 acctcagtat actatatgtg ttacaagaaa agactcaaaa acttctcgtc agtgtaagaa
      121 tgtttgaaac actatttaaa actaaataac tataagaagt agatcaggtg aacaatttca
      181 gtacctactc tataagctat acgaagatat ttttcaacat gatggataaa acgatcactc
      241 ttttaaagct atcttattta gaaaagagaa accatttaag catagatggg accttcttgc
      301 cctgttttaa atttttctta aactagagtc tgtaaaagat tggcttcatt ttcacatttt
      361 ctcctaggag gaatgtcgta atgcaaaaga attaaattca aataaatcat agcgatgacg
      421 actaatcgag tactcaaacg gagttccgtt atccaaataa gccacttgtt ctacttcaaa
      481 taccggttcc gttggaagga gctgcaagta atgttgctca ttttctgaac tagaagaagc
      541 tcgcaagatt tttgtagcac tggcaatatg caatcctaac tttccttcaa tgtaattgta
      601 aatcgatttt tgtaaaatat cttcagtaat acctggaata acatcggtac tcatataagt
      661 ttgttccaga acataaggtt taccgtcaat aatacgcaga cggtaaatat tataaactgg
      721 actctgcaaa gcgacctgta gtttttctgc taaaaattca tttgcaaatt ctaatttgaa
      781 gtgaataatc ctcgagtcaa ttgtagaatg agcatcttca gatatttttg tcagtccccg
      841 gatatctctt tctggaacga ttaattttct ttttgagctg ccacgagaga gaacaaaggt
      901 tccctgccca cgttttctaa aaattaagcc ctcagaaact aacaagtcta aagctttttt
      961 gatcgtcatt cgactacagg caaactgttt agctaatgtc atttcatcag gcaattgttc
     1021 gttcatggta tatttattac aaagaatctt ttcacgaatc tcattgtaaa taaataaata
     1081 ttttgtactc atttaatctg gttctctcta taatattcta atagtagtat aacaaagttc
     1141 aagaaatgag gcaaacacaa gacatgtatc aacaattttt aaaaatgtct agacaaattt
     1201 taaaattatg ctatacttta tgtatagaat aatagaaagg aggttgagca tgaaaaaaaa
     1261 gttagtattt cctaatctgt tttggtgggg agctgcttct agcggacctc agacagaagg
     1321 tcaatatgga aaagtacatg aaaatgtgat ggactactgg ttcaaaacgc atccagaaga
     1381 ttttttcgat aatgtcggac ctcttgtagc cagtaacttt tttcatactt acaccgaaga
     1441 tttccacttg atgaaggaaa ttggagttaa ttctttccgc acttccatcc aatggagtcg
     1501 actcatcaag aatttagaga caggtgagcc tgatccaaaa ggtattgctt tctacaatgc
     1561 catcattgaa gaagctaaaa agaaccagat ggatcttgtg atgaatttac atcattttga
     1621 tttaccagtg gaacttcttc aaaaatacgg tggttgggaa agcaaacatg tagtggagtt
     1681 attcgtgaag tttgccaaga ctgctttcac atgctttgga gataaggttc attactggac
     1741 aactttcaat gagccaatgg tcattccaga agcaggatac ttatatgctt tccattatcc
     1801 aaatctaaaa ggaaagggaa aagaggccgt acaagtcatc tataatctaa accttgctag
     1861 tgcaaaagtg attcaactat atcgctcatt aggacttgat ggaaagattg ggattatttt
     1921 aaacttgaca cctgcttatc caagaagtaa ttctccagaa gacttagaag caagtcgatt
     1981 tacagaagac ttctttaaca aagtcttctt gaatccagct gttaaaggaa ctttcccaga
     2041 aagattggta aaacagctag agagagatgg cgtgttatgg agtcataccg aaaaagagct
     2101 tcaactgatg aaatcaaata cggttgattt tcttggagta aactactacc atccaaaacg
     2161 tgttcaagca caagcaaatc ctgaggaata tcagacgccc tggatgccag accaatactt
     2221 caaagagtat gaatggccgg agcgtcgcat gaatccatat cgtggttggg aaatttttcc
     2281 gaaagccatt tatgatattg ctatgattgt gaaggaagaa tatgataata ttccatggtt
     2341 tatcagtgaa aacggaatgg gtgttgaaaa cgaagcacgg tttatcgatg aaaatggagt
     2401 tatcgatgac gtgtatcgta ttgaatttta tgaagaacat ttaagatggc tacataaagc
     2461 cattgaagag ggaagtcact gttttggata ccacgcttgg accgcatttg attgctggtc
     2521 ttggaataat gcatataaga atcgttacgg atttatctcc gttgatttag aaacgcaaaa
     2581 gagaaccatc aagagctcag gaagatggta tcgcaaagta agtgacaata acggttttga
     2641 agtagaaatt gaggagtaaa gagaggagat attcagatga aaaactcaaa atttatagat
     2701 caatttgcca cctttgctgg taaactaggg aaccaaattc atttaaaaac cctaagagat
     2761 gcattcgtaa cagtaatgcc attatatatt ttggcaggtt taatcgttct tttgaacaac
     2821 acggtattta agtggatttt ccaaggggat acattaacaa aattccaata ttggggaata
     2881 acaattgcaa acggtacttt aagtatttca ggtatgatta ttgctgtaat ggttggttat
     2941 ttcttagcta aaaacagaga tttcgaaaac ccgttagcag catcaatgct agcattagtt
     3001 tctttaattg tgatgatgcc aaatacagtt tctgtagttc ctgacggagc aaaagatgct
     3061 gtaaacattt caggtgttct ttcattcaac aacacaggta caggcgcaat gttcgccggg
     3121 gttatcgtag cgattattgc aacagaatta ttcattgaat tatcaaacgt taaagcttta
     3181 caaatgaacc ttggtgaaaa tattccacca gctgttagta gatcatttag cgtattactt
     3241 ccagtcatga ccgtcatctc cttatttggg gttgtttcag cattattatt caatataact
     3301 ggaatgaact taatctcaat cattacaatc tttattcaag aaccaattcg tcatattggt
     3361 acaagcttaa tcggggtcat tattatttac tctttaggaa atatgttatg gctatttggt
     3421 attcaccaag cagttattta cagtgccatc ctagaaccat tactattaat taacattact
     3481 gaaaacatca ctgcagcaaa taatggacaa gccattccac acatcatcaa cctatcacaa
     3541 atacaaacat tcgctttaat gggtggtagt ggatctacat tatgtttatt aatagcaaca
     3601 ttcttagtga gtcgcaatgc tgtctctaaa aacgtggcta aattatcttt tggacctggt
     3661 atcttcaata tcaatgaacc agtattattc ggttacccaa tcgtttataa catttcatta
     3721 gctattccat ttatcacagt tccagtcctt ggtattttaa tcagctactt agcaacagtt
     3781 acaggattca tgagtcctgc atttatacaa gttccttgga ctacaccagt attcttaaat
     3841 gcatggttag caacagcagg ggacgtgaga gcagttctag ttcaattcat catctttgca
     3901 cttggagttc ttctatacat tccatttatc aaagttaatg acaaagttgt tgaacaagaa
     3961 atggaaggtt aagattatga aacaaatttt attagtatgt aatgcgggaa tgtcgacaag
     4021 tatgcttgtt aaaaaaatgc aacaaagtgc gactgaacgt gggattgaaa tctctatcca
     4081 agcaaaatca atgacagaag ctaaaaagaa tatccatgaa gcagatgtca ttctaattgg
     4141 gccacaaatt cgttatgagt tacttgctgt gaaagaaatt gcaggaaata tccctgtaga
     4201 taccattgat atgcgggatt acggaatgat gaatggagca aaagttctgg aacaagcact
     4261 tgcatggata ggagaaataa gatgacagaa gaaatggaat atatctgttt tcagctcatt
     4321 gccaatagtg gagcagccaa atcatccttt attgaagcca tccaactagc taaggcagga
     4381 aatttaaaag aagcgaaaat taaagttgaa gaagcggaag actccttagt agaagcgcac
     4441 aagattcact ctaatctcat acaaaaagaa gctactggag aaaaaatagg attctccttg
     4501 ctatttatgc atgcagaaga ccaaatggct tcaacagaga ttattcagtt gctatctaaa
     4561 gaattcatcg atctctatca aaataaatag gaacactgtc attcccatct gtctttatta
     4621 atgacgacta cattgtagga acctccaaaa gttggacaaa atgattgaaa ggattggatc
     4681 ctgcattaca caagatccaa tccttttctt ttactctagt ttttataata tgttgaatct
     4741 agaatagtcc actgtagttt ctaaaaaatt gctggaaatg gatgttaagc tccaatttat
     4801 ttgtttatat cttatttcag tttactatac tagcttatta ttctagtgaa aaattgaatg
     4861 agccactatt tatttaatat gtatgatgag ggaatgactc aaaaacttct tgtcgatata
     4921 agaatatctg agatagtatt ttaaaacgaa aggactataa aagctcattt ccttcgcact
     4981 tattttatag agatctatca ttatactctt tagaaatcaa aaagcaacac accttgtcat
     5041 cgttttatcg taaatctgtt aatgaggggg ctagtcttat ctgcaatctc aaatcagtgt
     5101 tttgagctga atggcactcg ttttctagtt tgctctttga ttttaattgg gccttagtaa
     5161 atactactct taaattaaac tatagtgaat tgaaataaga tgtgaacaac tctatc
//
LOCUS       DAVLXF010000111         5215 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLXF010000111 DAVLXF010000000
VERSION     DAVLXF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.111"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            200..1075
                     /locus_tag="VYO12_001931"
     CDS             200..1075
                     /locus_tag="VYO12_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1588466.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNCKRSVKKSSVTKELKEKILHYHNQ
                     KFSPDKKQASINFKPAGQSIEQRPEAINFRLENGHYEIDTVLLTRAKNYCLLVLTDRK
                     SRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHP
                     YASWERGTNENRNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKYLDYKSPREDFLM
                     ANLNLKFSVRNKSRN"
     gene            1101..1745
                     /locus_tag="VYO12_001932"
     CDS             1101..1745
                     /locus_tag="VYO12_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1588467.1"
                     /translation="MLRAGVLDANNGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKAPARTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1787..3775)
                     /locus_tag="VYO12_001933"
     CDS             complement(1787..3775)
                     /locus_tag="VYO12_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1588468.1"
                     /translation="MFRLTNKLAISNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKKLGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTIFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLNQFPDSDIYNQFYGGMNVSISEEEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSVLFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQIINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3777..4535)
                     /locus_tag="VYO12_001934"
     CDS             complement(3777..4535)
                     /locus_tag="VYO12_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588469.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4963..5154
                     /locus_tag="VYO12_001935"
     CDS             4963..5154
                     /locus_tag="VYO12_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588470.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1618 a         1034 c         1024 g         1539 t
ORIGIN      
        1 gattttactg tcctgatcga tttgttctgt tcttgtttca ttttactata tgttcgtaaa
       61 tgaaagttct ataatgaagt tagccaccca gtcctcctaa aaattagatg gttcaactag
      121 ctgatagctc ttgtaggcat aagaaaacac ctctgtgtta tacttgttgt tcaaccacaa
      181 acacaagaaa ggcacagaga tgcaagacaa ttatactaca aaagccaaac atttgacaat
      241 cgatagccgt cgcttaatcg aaagatggaa aaaagaaggg aaatcaaata gagaaattgc
      301 ctctctgctt ggaaaagttc ctcaaactat ccacactgaa atcaagcgtg ggacagtccg
      361 acaatgtctt ggaaaagggc gcttcaaaga ggtttattct gccgactatg ctcaacagtc
      421 ttatgaaaac aattgcaagc gctcggtcaa gaaatcaagc gtgaccaagg aactaaagga
      481 aaagattctc cactatcata accaaaaatt ttcgcctgat aagaaacagg ctagcatcaa
      541 ctttaaacct gctggtcaat ccatcgaaca gcggcctgaa gctatcaatt ttcgcttgga
      601 gaatgggcat tatgagattg atacggttct acttacgaga gcgaaaaact actgcttgct
      661 tgtcttgacg gatcgaaaaa gtagacatca gatcatccga ttgattccaa ataaaagtgc
      721 tgaggtggtc aatcaggctc taaaactcat cttaaaacaa cacaagattc tttccatcac
      781 ggcagataat ggaacggaat tcaatcgctt gtttgatgta ttttctgagg agcacatcta
      841 ttatgcgcac ccctatgcct cttgggaaag gggaactaat gagaatcgca acaggctcat
      901 tcgtagatgg ttacctaagg gaaccaagaa aatgactccc aaagaagtcg cattcatcga
      961 aaagtggatc aacaactatc ctaaaaaata cttggactac aagtcaccca gagaagactt
     1021 cttgatggct aacttgaact tgaaatttag cgtcagaaac aaatcacgaa attgattcaa
     1081 attttgcagg tcgtttaaat atcctgcgtg cgggtgttct tgatgctaac aatggaatta
     1141 tttccattgc tggtgtggtt atcggagttg ccagtgccac gaccaatatc tggattatct
     1201 ttttatcagg ttttgcggct atcttagctg gtgccttttc aatggctggt ggagaatatg
     1261 tatccgtttc aactccaaaa gataccgagg aagctgccgt ttcgcgagaa aaactcttgc
     1321 tagaccaaga tagggaacta gccaaaaaat ccctctatgc tgcttatatc caaaatggag
     1381 aatgcaaaac ttctgcccaa ctcttgacta ataagatctt tcttaaaaat ccactcaagg
     1441 ctctggtaga ggaaaaatat gggattgagt atgaagaatt taccaatcct tggcacgctg
     1501 ccatttctag cttcgttgcc tttttcctta gaagtttgcc tccaatgctg tcagtgacca
     1561 tattcccaag tgaatatcgc atccctgcta ctgtccttat tgtcggtgtg gcccttcttc
     1621 tcactggtta cactagtgcc agacttggaa aggctccggc tagaacagct atgattcgga
     1681 acctagctat tggtctcttg accatgggag ttaccttcct gctcgaacaa cttttcagca
     1741 tttagaatac aagaaatacc tcgattttga agtcgaggta tcttttttac atttgcacaa
     1801 tcttgcgata acttcttgaa gtaatcatga aaatcagcac ataggcgatg aggaagatag
     1861 cgcagataga caaggtcacg atcaacatca tagtcgtatc cagtacacca atcactttta
     1921 aaatcaggct aagcatatgg taggcaaagg ctagatgtat gaaggcaaaa agcaaaggaa
     1981 ggaagaaaac agttaaaacc tgtttgttga tgatttgctt gatttgcttt tgatctaaac
     2041 cgactttctg caagataata aagcgttcac ggtcttcata gccttcagaa atttgtttgt
     2101 agtagatgac cagaacggtt ccgaccataa agataatgga taggaaaata ccaataaaga
     2161 agacaccgcc aaagaggaca ctcatttgag cactagcatc tgctagagtg ctaccataca
     2221 catagttacc ttcagtgttt aattgagcat taaacttttg taagtatttt tcatattctt
     2281 cagcgacttt gagttgctct tcttcactga tacttacatt cataccaccg taaaactgat
     2341 tatagatatc cgaatctggg aattggttca aaaaggcttg taaatcaggt acaacaaggt
     2401 aattgtaatc agcagtcaaa atattaaact gatttggaac atggttgaca ataaaatctt
     2461 tagtaaattc ttctttgaca gaaaattgat gatcatttag agttagagct ttctgttctt
     2521 taactccctc attctttgca aagagtccga cctcatttcc tgatagagac agtttttgac
     2581 cagtcatatt ttcataatct ttttggtcaa ataccatgaa aactgttttg ggttggacac
     2641 ggttttgtcc tttttcaaaa atagttaact tggttccttc ttgatttgca ataccaaagt
     2701 tactgtaacg aagtacttct ttctctttga cactataacc tttgtcactt gcaaactggc
     2761 tcaagagttt gtccaaatct tctttttcaa cattttgccc tgaaacccca aaatcatgag
     2821 gatttagaac ttttttaaag ctttctgcgg aattgaaaat gcttgtcgct gctgacatgg
     2881 ttaccaaaac cattgttgac aaaatagcga tggttgctag tccaaccgca tttttcttca
     2941 tacggaaaat caagttggaa acagatatga ggttattagg ttggtaatag tatttcttgt
     3001 ttttctttaa gatttgtagg aagactgtaa tccctgcatt aaacaataga taagtaccaa
     3061 agataaccag caaaacagct aggaagaaaa ttgttagggc tgtaagagga tcggttaccg
     3121 taagggcaag ataatagcca atccctaaac ttatggaacc aagaatagtt tggagaggta
     3181 ggaaacgacc tcttttctct ccgctagctt tctcacgtga gagctggagg gcattcatac
     3241 gggcgattcg aagggcattc aggaacatga ggcctaggaa aatcaaaccg aagacaagaa
     3301 gtactgtaat gacaactttc atctggaagg tagcgaccag ttccaccttc aatttcatca
     3361 gtttgagtag gaaagcgaaa atcaacttgt caaacaaggc tccaataccg attcctgctc
     3421 caacagttag aatcccaaat agcactaact ccttaaaggt catactgatt agatggcgct
     3481 tctccaagcc taacatgcca tatataccca gtttcttgga acggtttttc atgacaaaac
     3541 tattggcata gaggacgata atggctgacg caagggtaac gacaaacata ccaaatccaa
     3601 gtgttgcttg aatggtggtt cctccacgga tttccgcaat ctttggattg aaggttaggg
     3661 agtaaaagag ataggtgatg gtgactgcca agagaacagc cagtgcaaag ggatagtaga
     3721 gtttgcggtt tttaatcagg ttcgatatcg ctaacttatt ggttaatcga aacatactaa
     3781 ttcacctcgc ttgccatgac agtcaaggta tcagagattt cttggaacat ctgacgctct
     3841 gtcttctctc cacggtagat ttggttgtaa agaatgccgt ctttgataaa gagaacacgc
     3901 ttggccctgc tagcagctgc tgttgagtgg gttaccatga ggatggtttg cccacgctca
     3961 ttgatttcat taaagacatc aagtaaggct gcagatgact tggaatcaag ggctcctgtt
     4021 ggctcgtccg caaggagaat ttcaggttct gtgatgatgg cgcgggctac tgctacacgc
     4081 tgtttctgac caccagaaat ctcgtaaggg tacttctctt gcaattggtt aatacccaga
     4141 ttctcagctg tcaccaccaa tttcttcatc atctctgtaa taggtcttct tgacaagaca
     4201 agcggaagca agatattgtc ctttacagat agagtatcta gcaagttaaa gtcttggaag
     4261 acaaagccca acttttcacg acggaagcta gaggcctgtg aatttttaat ggttgcggtg
     4321 tcagttccat tcaagtaaac ctgaccacga cttggtttat ccagcatagc tagaatattg
     4381 agaagagttg atttaccaga accagactca cccatgatgg caacgtagtc acccttttcg
     4441 acggtaaagt gaatatcctt gagggcttct acttggttgc cctgaaaacg tgttttataa
     4501 attttttgaa cgtgttttac atctaaaagt gtcatgagaa tctcctttct taatatccca
     4561 tcaaatgaaa tgtagcttgc atcatagtgc atcccagctg aacgcgctca atttctttgt
     4621 gacttggttt tctagttttc ttatgtaaga tttgtttcat gatgttactc ctttgttctt
     4681 tatgagtcta gtttacatca aaaaaagacc gcttgccata acctaacctt acaaaagaga
     4741 ctttaatctt acatttttgt aagattgggt gaaatataag caaaattact tagaaaatga
     4801 tttgatttcc tatttttaaa gttgtaaaac ataagtgtga aaaggcttac tttgaaaagg
     4861 aggaagcggg aactatcatt tgagtaagct tacctagata acataccatt caatttaaca
     4921 cttgcaagtc gttcaaccta taaggaaaga aggtgcgctt taatgaaaca caaagaacat
     4981 atactcatcg gacttctcta tctcctctct ccattcatcg gtcagctctt agttgaacac
     5041 acacacttta tcggtacaga atttaccggc actgcttatg ttatttgctg gttatcagtt
     5101 gttatcagca tccaccattt ttccaaaaat gtattgtctc agcagcaaaa atagattaaa
     5161 aaactgagtt taaattactg cttttataat aaaatgaaat aaaaacagga caaat
//
LOCUS       DAVLXF010000112         4878 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLXF010000112 DAVLXF010000000
VERSION     DAVLXF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.112"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(87..1637)
                     /locus_tag="VYO12_001936"
     CDS             complement(87..1637)
                     /locus_tag="VYO12_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1588471.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(1653..2432)
                     /locus_tag="VYO12_001937"
     CDS             complement(1653..2432)
                     /locus_tag="VYO12_001937"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1588472.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(2419..3270)
                     /gene="ylqF"
                     /locus_tag="VYO12_001938"
     CDS             complement(2419..3270)
                     /gene="ylqF"
                     /locus_tag="VYO12_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1588473.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEKAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(3351..4859)
                     /locus_tag="VYO12_001939"
     CDS             complement(3351..4859)
                     /locus_tag="VYO12_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1588474.1"
                     /translation="MYYVTKTNSKGQPLYQVVEKYKDPLTDKWRTVTVSYTRNTSRAR
                     KQAEREVMDKIEKLTSSLESQYNPEMITTFGELKESWFQTWCVSVKPQTIKREELVIE
                     RLGKIIGDEFLLDRITPLLMKNCLNKYLDKYDASPSTMTHIKSTCNKIFNHGVLYNVI
                     KYSPMTAVKIDVPLEKKRESRKRLDAKFLAIHELQAFFDVLGQCRNASYYDLAIVLLL
                     TGIRIGEAAFLPSDVDFDKGILHIDKALQYHDLKVDEFYFDTTKTVNADREVALPIAA
                     SEAIQRTIKRNQEFEAYMEQNPSPAFKKCDSVFQTEYGSPITSHSFRQVLKRVEEKLL
                     KNCQKDYGFKWVKHVTPHSFKHMHISYLQSNEMHIAVKDIMTRVGHANFETTMGYTHN
                     ISRSQENTVTALNQFVEKHNFHFKELKSYTCKYSRMIEQFIETCDDNNKVELSVDEFK
                     DLLHLGSRYSPKHIVSNLLLKIKKDIVKYHPQFDIKIVKLSGNQIKGFSISW"
BASE COUNT         1440 a         1004 c          897 g         1537 t
ORIGIN      
        1 aaataaccga tctcatttct gagaatcggt tttctaaata aatcaagctg agcatttaca
       61 taacataaat tatgtaaatg ctcatactac aacaacaaat ctttgacttt tccaatttca
      121 ctttttggaa taaccaggtg aatcatatca cccagataca ttctggttga gccgttaact
      181 gtttggctct tgccattatg gacttgagtt gtgatgagga cgttgtgtgg taagttgagt
      241 tcatgaactt gtttcccagc aattttatca gaaactggta tttcgataag tgtaacttct
      301 ccttcgctag atacttcttc tggaagcatt ttttccagca tggcttcata gactggcgta
      361 cctttgagca aatccatgat aatataagaa acaagagtga caagaccaag tggcatgagg
      421 ttgcgaatat ctcctaccat ctcagttacg aggatcatag cggttaaggg agcttttgat
      481 atggctccaa aatagccact cattcctaga atgacaaata tagggaattg ctcttgactg
      541 acaagtccaa gattgacaca gataacacca actaaggcac caagcaaaga accaagagct
      601 aaaatgggga ggaaaattcc tcctggcagt ccacttccat agctaatcat actccaaata
      661 aagcggatca aaaagtaagc taataaaact tggaaactaa aattttgttc agttaaagaa
      721 aggacaagct gatttccgcc accaatgatt tgaggtaaga agattccgac tggtatgata
      781 aggataaaag ccaagatggg ataataagcc ctatccaaat ggattttttg accaatcaag
      841 tcataaactc ttccaacgtt taatacagct ttctcataga gaaaacctga aaatccaagg
      901 aaaattccca tgacgagata tatccaatac tggtctaggg tcatgggagg aatgttatct
      961 ggcatatcca atactggtgt caaaccgaac atgagtagag acacaaagtt tgctacgatg
     1021 ctggctgcta gagttgagac ccagaaaaag cgcgaaaaat ggtgatagac ttcttctaca
     1081 acaaagagaa gtgctgcaat aggagcatta aaggctgcgg ctaaacctgc tgcagctcca
     1141 ctagcaatca aggaacgttc ctctactgga ctggatttga gccacttggc aattccttta
     1201 ccaccaactg ctccaagttg aatgctgggt ccctctcgac ccagcatgag tccactggca
     1261 atagcaagaa tacctagcac atattttttc caaagaatgc cccaccagtt gagggacatg
     1321 aggcctttca gttcggcttc gacttgagga attcctgagc ctttaatatc tttttctgac
     1381 cgtgttagtt tggcactgag ccaacagatg agtatataaa acaaaaccag tacaaaaaga
     1441 ttgcgcacta agtacccttg atcttgataa actccttgta tcaggtggaa gcccttttca
     1501 attaagaaac gaaaggatcc gacgataatt ccaacgatga ggccgacaat gattcctcga
     1561 ccaacttggg atagtatagt gctggatgca aaggcgaatt ctttcttaga acggactatt
     1621 tccgattgtt cctccatggt atttcctttc aattaacttt cttttttacc taaaaccagt
     1681 gatttaacgg gttcaaagct ggttcggtga attggggtaa ctcctagttt tgtgaggcct
     1741 tccagatgtt tagctgtgcc atatcctgca ttagtagcga aatcatagcc agggaactgc
     1801 tgatcgtatt ccttcatcaa ttcatcacgt gttaccttgg ctactataga tgctgctgcg
     1861 atagagaggg agttggcatc tcctttgata atggaggttt gtgaaatggg caagtccagt
     1921 ttcatggcat caatcaaaag gtgctctggt tgagggctga gctgggagat tgcttcttgc
     1981 atggctagtt tggttgcttc atagatattg acttggtcga tgacctgatt atctatgata
     2041 ccaattccaa tcgacaaggc ttggtcttga acgacttgga aaatctccag atgtttcttt
     2101 ttaggaattt tcttgctgtc gttgagacct ttaatcttac aatttttagg taaaataacg
     2161 gccgcagcga ctacaggacc agcaagagga ccacgaccaa cctcatcaat acctgcaatt
     2221 aaggtcaatc cttgcttata aagttctttt tcataagaaa gcatggattc caagcgcaaa
     2281 ttttcatcta attcagcttg gatggctctt ttacgcttgc tgatttcctt ttgaactcct
     2341 gagcgattat ccttttcaag ctctaaaaaa atagggcttt ctaactcctt gactgtcaca
     2401 aggaattctt tgatttcttt aatcgttgcc atcgaggtct tccaatgtat ctaaggtata
     2461 gttaccgagt ttgccatcac ggacttcctt cacgaagaga ctgtaaaaac ggtcatagtc
     2521 atcacggaaa ccgagggcgc gggtcatatc cataataatc acaggcgctt tttcttcaat
     2581 tttcatttgt ttgaagcgtt cagccagctt ttctggataa tgttctttga aataattgat
     2641 accaaaaatg gtaacctcat ccataggaag caactggtct ttgatagctc cagtcaatgc
     2701 caacttaagt gcaacagttt catcctcaaa cttaggccag agaatccccg gtgtatccaa
     2761 gatttccagg tctttattgg ttttaagcca ttgttgacct tttgtgaccc ctggcttgtt
     2821 tccaacaaca gcaatctttt taccagccaa acgattcatc agagttgatt taccagcgtt
     2881 tggaatcccg ataatcatag tacgcaaggt ttcaatctga atcccacgtt ctttctggcg
     2941 agcaatctta tcagccatga gcttcttggc cgcatctgtt acaactttta cagtcacttg
     3001 ctctttggag ttgatagcta gcgtctggat tccttgtgat tcaaaatact gacgccattc
     3061 cttggtcatt gctggatcag ccaagtcggc cttgtttaaa atcaagagtt ttggtttatc
     3121 accaacaatc ttggtcaaca taggattttg actagataga ggcaagcgtg catctactaa
     3181 aatcgtcaca aaatcaacaa attttaaatt ctcctgcacc tgtcgacgag ctttagacat
     3241 gtgaccagga aaccattgaa tagtagccat tgagtttttt cctttcgtag caagggttta
     3301 gcacccctca tttattttac tatcgtctaa acaccaagcg aacaccaaaa ctaccacgaa
     3361 atggaaaaac ctttgatttg attcccgctt aatttcacaa tctttatatc aaactgtggg
     3421 tgatatttga caatatcttt tttgattttt aacagtaaat ttgaaacaat atgtttgggt
     3481 gagtaacgtg aaccaaggtg taataagtct ttaaattcat caacacttaa ctctacttta
     3541 ttgttatcat cacaagtttc aatgaattgt tcaatcattc tcgaatattt acaggtgtaa
     3601 ctcttcaatt ctttaaagtg aaaattgtgc ttttctacaa attgatttaa agcagttaca
     3661 gtattttctt gtgaacgact gatgttatgg gtgtagccca tcgtggtttc aaaattcgca
     3721 tgaccaactc tagtcataat atcctttaca gctatgtgca tttcattact ttgaagataa
     3781 ctaatgtgca tatgtttgaa agaatgaggg gtcacatgct tgacccattt aaaaccataa
     3841 tccttctgac aattttttaa caacttctcc tcaaccctct tcaaaacttg acgaaatgag
     3901 tgagaagtta tcggagaacc atactcagtt tgaaatacac tatcacactt tttgaaagca
     3961 ggactaggat tttgttccat ataggcttca aattcctgat ttctttttat agttctctgg
     4021 attgcttcac tcgctgctat tgggagggct acctctctat cagcattgac ggtcttagtc
     4081 gtatcaaaat aaaattcatc aacttttaaa tcatgatatt ggagagcctt atcaatatgt
     4141 aaaatcccct tatcaaaatc aacatctgat ggtaaaaaag cagcctctcc aatccgaatc
     4201 cctgtcagta ataatacaat agcaaggtca taatagctag catttctaca ctgccctaaa
     4261 acatcaaaaa aggcttgcaa ttcatgaatt gcaagaaatt tagcatcaag cctttttctt
     4321 gattctctct ttttctccag aggcacatct attttaacag cggtcattgg agagtactta
     4381 atgacattgt atagcacacc gtgattaaat atcttgttac acgtactttt tatatgtgtc
     4441 attgttgaag gtgaagcgtc gtatttatcc agatatttat taagacaatt tttcatcagt
     4501 agtggtgtaa ttcgatcgag taaaaattca tcaccgataa ttttaccaag ccgttctatc
     4561 accaactctt ccctttttat tgtctggggc ttgacagata cgcaccaagt ttgaaaccaa
     4621 ctctctttta actctccaaa agttgtaatc atttcaggat tgtactgact ttcaagggaa
     4681 gaggtcagtt tttctatttt atccattacc tctctttcag cttgctttcg tgctctacta
     4741 gtatttctag tatagctgac agttactgtt ctccacttat cagtaagtgg atctttatat
     4801 ttttcaacca cttgatacaa gggttgtcct tttgaatttg ttttagttac ataatacata
     4861 atttaattcc tcgaaaag
//
LOCUS       DAVLXF010000113         4858 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLXF010000113 DAVLXF010000000
VERSION     DAVLXF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.113"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(298..1524)
                     /locus_tag="VYO12_001940"
     CDS             complement(298..1524)
                     /locus_tag="VYO12_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1588475.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISEILPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1596..2261)
                     /locus_tag="VYO12_001941"
     CDS             complement(1596..2261)
                     /locus_tag="VYO12_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1588476.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2273..2950)
                     /locus_tag="VYO12_001942"
     CDS             complement(2273..2950)
                     /locus_tag="VYO12_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1588477.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            complement(2972..3205)
                     /locus_tag="VYO12_001943"
     CDS             complement(2972..3205)
                     /locus_tag="VYO12_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588478.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            complement(3491..4354)
                     /locus_tag="VYO12_001944"
     CDS             complement(3491..4354)
                     /locus_tag="VYO12_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1588479.1"
                     /translation="MIERMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRFGRKVVDRFAHQDIVGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKSGFLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIVLFE
                     LRLQQYKKLID"
BASE COUNT         1710 a          946 c          839 g         1363 t
ORIGIN      
        1 aaccatacct acggcaaagt gaagctgacg tggtttgaag agagtttcga agagtataag
       61 tagactgaaa agaatccagc cacagcatgg actattatat agcagattga aataagatga
      121 gaacaaatcg attgggaaag taaaattaat ttctataaat tttttagcaa ttgtttcgta
      181 ctattttaga ttcagtctat tataacacat tcagaaaaga gaaaaaagtc tgttgatttt
      241 gaccatcata aaaagactgg caatccagtc tcaaacatat attatagaaa ttctccacta
      301 aatactttca cgaatattca gaagcataac aaaggcaact agaagaaata gcaataaaac
      361 aaagctaact gccagagttc caaagctagt agcaatggtt accaaagcta ttgtaaataa
      421 gctaggtaaa acaaccgtaa tggcaccgat agaggattga actgctccca ttgactcctc
      481 aggtatttgt ttaaaaacga gttcttgcaa tctaggagag agaacacctg cgaaaaaggc
      541 atccaaggta ctaaagatga gaatccagtc aaaacgaact gtggcaaatc ctactagaag
      601 aagcaactgg atgacaagtg aggcatagag agctgttttt atggaaatgg tatgttgcag
      661 atagccactt acaaggcttc cgacaatcag ggctgacagt tcaaaagtgg atagcaaggc
      721 aagagattga cctgtttgta aattcaaaaa gggttgattc ctcaaaaata gagtagagac
      781 aggaactgta acatttatca ctgcttgact agtagagata ataaacaaaa ccaagagcac
      841 cttattcata ttccatatca atttcgatga ttggagcaaa tgctggcaaa aggattttac
      901 agagagtcct tcttgatagt taatcgtttt ttctactttc aagaggtcag tttttatgaa
      961 aaggatacct aaaaatgcga ttaaaaaggt aagagcgttc agcaaggaaa tgaactggat
     1021 ggagagaatc cctagcaaga ctcctcctag aatattactg attgttttca ctaaactaac
     1081 agttgactgt ttaaatccaa tagcttctgc cagatggtct tgcccaataa ttctaatgaa
     1141 aatcggagtg agcatggctc ctgaaaagta actcaatgtg tcagacaaga ggttaatcag
     1201 acaaataaat gctactagca acaaggagaa agactgccct gaaagtgata aagaaacgat
     1261 agagtaaagc aaaaattttg caaaactaat gactgtgtat ttcaagacac gatgatgttg
     1321 aaaatctgcc aaaactccca gaaagatttg taaaatttgg ggaaggattt ctgaaatcgt
     1381 aatgagtaaa atcgccaaag gtgcaaaaga cgcatctgcc acataattca gaaaggccag
     1441 ataaaaaatc gtatccccaa gcgttgaaat ccactggttg atagttaatt gcctaaaatc
     1501 tctattttga agaaatactt tcatcacaac tccttcttaa gttcaaatgg gaatctttcc
     1561 ccagaaatag accgcgatac tactaacaac caaaattaca gtaacatcaa aagctgacca
     1621 atgccgttgt agactatatg cagcccaata ggccaataaa tggactttgt cattctaaat
     1681 aagactgcaa atataagtcc gccacccata tagacgaaaa agtctgtcaa gacccaaccg
     1741 tgattactaa tgtgcgagac cccaaataaa atagcagaac caagtacatc tagcccccat
     1801 ttctttcctt tttccagagc tgtcatcacc aatccccgat ggattatctc ttcaaaaatg
     1861 ggacctgcaa tcacaggata gaaaaaatac atcaaaaatg ctgtagcccc tgtaaaagtc
     1921 ggagcagcat gttgataaga aatttcattt cgagtaggtg ggaaaagaaa aaaggtaacg
     1981 aaattccaaa caacaaaagc aagcagagct aggaaggaat agaaaagata ggatccttta
     2041 aactttctac tattgatttt ctgccatttc cccgaccaaa tcatagcaat aagagcaaat
     2101 aaaaccacaa gaaaattcaa catcatatcc gacagataat aggcaaagtc agatagccca
     2161 gtaacaaggt cgctgcgtaa aactagaaca ctgaacttct ggtcagcaat aactagtaga
     2221 aaaactataa taaagtagcg gtgtgagatt atctttttca tatatcacct ttctaatatc
     2281 caaataccaa taaagtaaca atgagtaaga aactattcca tgaagcatgc agagctatag
     2341 cccaatagat ggatcgggtg tagcgaaaca tcatacaaaa tatcaagccc attccaaaat
     2401 actttatgaa atctgtcgtt atccaaccat actgcaaaac atgcatagcg ccaaatatgg
     2461 cagcggaaac aagaacatca agatagtatc tcttaacttt agataaactt gtcatcaaaa
     2521 gaccacgaca aacaacctct tctgatacag gtgcgataat actagtataa agtattcgcg
     2581 taacaaaata gctaattcct gttaaattgg tggctacttc tacgactgta cttccattct
     2641 gggtacgagg aaagatatag gttgttagat ttgcccacac gaacaataag aaaaaagaaa
     2701 gaaggaaaac acccaggtaa gaccaacgaa actggaaacg accacactct ttccaatgtt
     2761 cacttttgac aaaagcaatt gtagctatag ttcccagaat aagtaccaat aaaacttgga
     2821 acacatagta catattatca gacaaagcaa ccataaaatc taagtctgat gtgacattaa
     2881 aaatgaggta ataagtcaaa atcaacaagc cagttgctag gtgaaatttc acttctttca
     2941 ttttcttcat cctattatct cctataagag cctatcttct acggcggcca aacaatccat
     3001 ctgctaaatc tatagtccaa tcaaaagctc cacgattagg actcatccct tgattgcccc
     3061 aaccagggta aattcctggg acgccccaac cagatatacc acttcttcca ccacctccca
     3121 tagaatttac gaggttgcct cctctaacat cttgcaactc agcttctgtc aattccattg
     3181 tttctgcaaa ttgtaaattt aacatctttt acactccttc aattatcttc atttgtaaac
     3241 cacttctgcg acctaggatt tgcttcaagt gctttacaag tacagtataa cacgaaaatt
     3301 ggcttatttt agaaaatcgc atatttgata ttttttctta tagaaatttc agatttgcga
     3361 ttttggtgaa tttgattact tctctggtat aataaagtta ctactaatga ggagtggaga
     3421 aatatgaaga aacaaatttt aacattattg aaaatcgttg ctgagattat tattattttg
     3481 ccatttctaa ctaatcgata agctttttgt attgctgcaa acgcaattca aaaaggacta
     3541 ttaattgtgg attttctaat acttgcagag attggataaa gtgttcaatc tctttttggt
     3601 tgcttccctt ggtttgaaga aaaatactca ttttctttaa aaactgccac aacactttct
     3661 caaaaacatc atacggacgg agcatactct ccaactcggc ttcaaagatt gggatgtaag
     3721 agaaaagttt gcgctccatg agctctgata taatattcag aaagccactt ttcatataca
     3781 gtctattatg taccaactct ttaaattcct tagatttttc aagcaaagag gttgataaaa
     3841 aaatcaaatc ttggttgctc aagaagggca tggtattgca aaagagatag agttcaaacc
     3901 aggtccaaga ctcgatagca tagagatagg tggttaaaaa ctcgctatcc tcctctgcta
     3961 gcggatagct tttatctaag gaatggatgg catctttaat cacgatggca ttcaaacgac
     4021 gataggtctc tgccatctgt tcttgctcga cttcctccaa tagttgttct aagccaacta
     4081 tatcttgatg ggcaaagcga tccacaacct ttctaccaaa tcgcatatgt ggagattctt
     4141 gatagttgtt gagcttgtgc ccaaactcat caaaattcat attgatccct tggatggcta
     4201 atatgagctt atctgcagac aacatagact gccctagttc aaacttggac aactgagagg
     4261 ctgttagacc agcacaagcc acatctgact gcttgagctt tctagccagg cgcagttctt
     4321 tgtaaaattc tcccaattcc attctctcga tcatctcacc acctcctatt ttttatatag
     4381 tatatcattg tttttatagt tttgtcaaaa cattctgaca ctttggtgat tgaaaaagcc
     4441 agccttaagc tgactctaaa aggaccgagc cttgtacaat acagaactca atcctcgttc
     4501 taaatagaat gtctaacttt tttgggcagt acacgagctt ggggaacagc cactggaacg
     4561 atgaggtgtg agctcaaaat atcctccagt tatgtttttc ctaatagtat accggaagag
     4621 tgaaaggatt ttataatgga gcggttacaa agaacctact ttctattaaa cagtatacta
     4681 tgaaaatgtg aaaatttaac atttttttgt acaaatttta taaattattg cctttttaat
     4741 atcaatagtt aatctcttat ccagatcccc cttgtgtaaa ctttatcttt ataagcttca
     4801 aggcccctat cccatctatt tgcaacaatt agatcacttt gttttgtaaa tagttcaa
//
LOCUS       DAVLXF010000114         4807 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLXF010000114 DAVLXF010000000
VERSION     DAVLXF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.114"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            125..270
                     /locus_tag="VYO12_001945"
                     /pseudo
     CDS             125..270
                     /locus_tag="VYO12_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
     gene            397..1119
                     /locus_tag="VYO12_001946"
     CDS             397..1119
                     /locus_tag="VYO12_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1588480.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEKDIEIDKEREGEIDKKSTP
                     IFYGEFKNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEE
                     DKDKLRQKGLNKKMNYDVGESL"
     gene            1803..1964
                     /locus_tag="VYO12_001947"
     CDS             1803..1964
                     /locus_tag="VYO12_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588481.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQDMKNSFD
                     MAKTQIETD"
     gene            2056..3735
                     /locus_tag="VYO12_001948"
     CDS             2056..3735
                     /locus_tag="VYO12_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1588482.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSDKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIIEELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYKEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            4003..4569
                     /locus_tag="VYO12_001949"
     CDS             4003..4569
                     /locus_tag="VYO12_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588483.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT         1960 a          611 c          957 g         1279 t
ORIGIN      
        1 aactaataaa gaaaaatatg acttcaaaaa gctaacaagt gcatatagtt atttaagaaa
       61 aaacaacata gaaaccatag gtcagttaca aacaaaaata gaaactttaa agtctaatag
      121 ttataggcta aataaaaaag aaaaagaaag tatccaaaaa caaataaaga aatacaaaga
      181 cctatccaaa aacaaagata agacaaaaga aaaaggaaag taaaagaaaa agtaaaacca
      241 aacaaaaaag atatgaccag agaaatctaa agggagtaac gaaaagttac acccttaaat
      301 aaccataata acaagacttc cgaaaaacag aagtccagaa ttccgaaaat cggaaatcaa
      361 gaatttcgat tatcgaaaat caagaaagga gcagatatgg caacaaacaa aagatactat
      421 tggataaaat taaaagaaga atttttcaca gacaaaagga taaaaaggtt aaggagaata
      481 tctggaggag atacctatac gataatctac ctaaaacttc tactactaag cctaaaagat
      541 gagggcaaac tctattatga cggagtagaa agtgatttta taaaagagtt agcactaacc
      601 atagatgaaa cagacgatga tgtaatggta acagtaaatt atttaatcaa tcaaggctta
      661 ttagaagttg taacagaaaa tgatgaatac tacctaactg aaataccaaa cttaatcgga
      721 tcagaaacag cctgggcaga gaaaaaaaga agatacagac aaaataaaca gaggacattg
      781 tccttaatga gtccaaccca tgtccgacaa gagatagaga aagatataga gatagataaa
      841 gagagagagg gagagataga taaaaagtcc acccccattt tttatggaga attcaaaaat
      901 gtaaggttaa gcaaagaaga atataaaaat ctaaaagaaa aattaaactc acacacagat
      961 ataatgataa ataaactatc cagatacatg gaaagcagtg gtaagaccta tcaaaaccac
     1021 tatgtgacaa tattaaaatg gtatgaagaa gataaagaca aactaagaca gaaaggttta
     1081 aataaaaaaa tgaattatga tgtaggagaa tctttataga taaaaagagg tggaaaggca
     1141 ctattaaaga ctttaagtct aaaaaggtat caaagtatgc cagagataaa aaaatataaa
     1201 tatgacactt taaaggcgat tagaaaatta aagaaatgct agaagacttt tagatgacag
     1261 ttaccaagag ggtctaattt tcactgctac ttatgcagtg ggaattgaac ctttttatat
     1321 aagcaaattc tatttcaaaa ttggttacca agaaagactt cttttcactg ttaagtataa
     1381 gagggtaatt tatagaattt tgttacatca agataaatat tttctgatgg tcaaatttta
     1441 aaaagattaa taactctcga taaattttga accttaacaa ttgaatagac caagacaaga
     1501 cgttaattat tttttgagcg taataaccta tccgtcaaga tctttcggct gaataattcg
     1561 gcaaaaaaag ttctgctaag caaaaagaaa gtgagcgata agtaagagtt tgaatatagg
     1621 gagggatacc ctatccgcta tgaagaaaat gaggataatg atacttctaa ggttgaagcc
     1681 ggctcatccg gaatagaggc ggaggtgaga ttcctatgtt gctaatccaa agcacttgtc
     1741 aatgtcaaaa aacttaatta aacaaataat tattagatat aaaactttag aggaggtata
     1801 tattggacaa tgactggaat ggattggctg atttaatagc aaatctgatt gcaaaatatg
     1861 ctggtgcttt agatttagat aatctacccg atccaacgcc agcaaaaaat caagatatga
     1921 aaaatagttt tgacatggca aaaacacaaa ttgagacgga ctaataaaag tgttataata
     1981 tacttgaaat gatagtccaa acttaaatta aaaaaattat atatgatata gaaaaattat
     2041 attgaggtta atactatgtc aaaagaaaaa ataaaagtat acctctatac acgagtatct
     2101 acgtcaatac agatagaggg gtattcgtta gaggcacaaa aatcacgaat gaaagctttt
     2161 gctatttaca acgattatga aattgttgga gagtatgaag atgccgggaa gtctgacaag
     2221 tctattgaag gtagaataca gttcaatcga atgatggaag atataaaatc tggaaaagat
     2281 ggagtatctt ttgttcttgt gtttaagtta tcaagatttg caagaaatgc cgctgatgtt
     2341 ctttctacac ttcagataat gcaggattat ggagttaatc ttatctgcgt tgaagatggc
     2401 attgattcat ctaaagatgc tgggaaacta atgatttctg ttttatcagc tgtggctgaa
     2461 atagaaagag aaaacatccg tattcaaaca atggaaggtc gcattcaaaa agcaagggaa
     2521 ggaaaatgga atggtggctt tgctccgtat ggatataaac ttgaagacgg caagctgttt
     2581 ataaatgagg aagaggcagt tgcaataaga acgattttcg accagtatgt aaatactacg
     2641 ataggagcca atgggatatc taaatactta gagaatcatg gaattagaaa aatcccaaga
     2701 cagaatggta agaatccttt gtttgatgca ggtcttataa gaaagatatt aaagaatcct
     2761 gtatataatg ggaaaatagc atttggaaga agaactttag aaaaagttca tggtacaaga
     2821 aatgaatata agcaggttga acaagatgaa tatctaatat ctgaagggat acatgaagct
     2881 atagtttccg atgaagtttg gcaagctgct caggttaagc taaaatctca agcaaagaaa
     2941 tatgagcatg tgaataaagg aaaagataca cgcacacact tgctttctgg aattgtaaaa
     3001 tgcccgatat gtggagtggg aatgtttggg aacaagtgta tcaagaaaaa gaaagatggc
     3061 acaaagtata aagattttta ttactatggt tgtaaacata ggcagatgat aagaggtcat
     3121 aagtgtacat tcagtaagca aattatagaa gaattgttag atgatgctgt tgcagaggtg
     3181 attgtcaaga tagtaagtaa tccgaaattt gcttctatga tgcaagaaaa aatcaacatg
     3241 aaggtggata cctctgaaat agaaaaagaa atagataatt accaaaagga attgagaaag
     3301 agtcattcta caaagtttaa gctaattgag gaaatagata atttagatgt tgaagataag
     3361 cattataaga gaaggaaaca ggatttagat gatagactat atcgtatgta tgacaaaata
     3421 gatgaattag aatcatcact aattgatgcg aaagcaaaga aacagactat tgaagctgaa
     3481 aagcttacag gagataacat atataaggtt ctgatctatt ttgataagct ctataaagtc
     3541 atgaatgatg tagagcgtag gcagttaatt tcagctttga tttctgaaat tcaagtttat
     3601 aaagaaaaac aatctaacgg acaatggcta aaatccatta cttttaagct accaattata
     3661 gaagaaaatc tcaacatagg tttggacaat gatgaacaag ttgaatgtgt aagtttatta
     3721 gagaaacgta gttaatcctc aaaaggttcc ccgaaccttt tgagtcccac tgacgcatca
     3781 cgctgaggcg gtatcactgc ttgtacgagt tgaagcatca gcaaagtaaa agtagatgtt
     3841 cagccaatgc gacgaggtga gaccgaaggt cgataaggtg tgttctgggt ataggactcg
     3901 taaagtaagt aggaaatatg atgagatgat acagagagcc tgaattggtc aggtgacgtg
     3961 cttatttaca ttagcctgat aatgaaaatg gagagattat cgatggttat attggaattg
     4021 taccaaaatg attattctaa agatttagta gcatttgatt ccatagaaga tgggaaagcc
     4081 tttgtagctc aaatcccggg atacactctg gaaacagaag atggttttga agtggagtat
     4141 tttaatccta aaaacatacc ggattatatg gaaattattt ttaacggaaa tattgttcct
     4201 ttatctaaat ttatgttcga tcctggggaa aatgtaaaca ttatttggaa agagatttca
     4261 aatttatccc tcaaaaatga tagagtgatc gaaggatatt caaaaattga tgcatatgtc
     4321 gttaacaacc atgaagttaa agcctatgtc gaaacaagag aaacgaacta tcgtaaagca
     4381 aaagacttct tagaaagccg cggatatgaa atcgacagaa gctttttcgg aagtgaagac
     4441 ggtgaagcga ttctttacag aaaaaaaggg atcgaagttt ggcatttctt gtgtcactta
     4501 gacccaatgt ttgtagagat tgaagatgta gaaggatatg ttaaagaaga agttggagag
     4561 attcaatagt caaccaaagt tccttgtgag tatatacctt tgctagtaaa agagtcaaaa
     4621 cgttataacg aaggggggga agaaaatcct tgagtgtaag tctttttctt gtttagaggg
     4681 aaagtattag agtagccgct cataatttat actagatttg ataactgatt tgagacagat
     4741 tttatttcta taaatattgt gaaaattttt taataccaaa gatttaaagt tactgtgtaa
     4801 aaaatcc
//
LOCUS       DAVLXF010000115         4329 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLXF010000115 DAVLXF010000000
VERSION     DAVLXF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.115"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(75..1490)
                     /locus_tag="VYO12_001950"
     CDS             complement(75..1490)
                     /locus_tag="VYO12_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1588484.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTLNRY
                     KKMSFTWYKEVIESNGESLFK"
     gene            complement(1503..3341)
                     /locus_tag="VYO12_001951"
     CDS             complement(1503..3341)
                     /locus_tag="VYO12_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1588485.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTLAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3359..4198)
                     /locus_tag="VYO12_001952"
     CDS             complement(3359..4198)
                     /locus_tag="VYO12_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1588486.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELAYLMAHIRRLTK
                     NLD"
BASE COUNT         1441 a          850 c          568 g         1470 t
ORIGIN      
        1 cttccaaact tctttattcc atatttaatg aaatgccacc ttaaccgtga taatagctag
       61 tcatcaataa aaaactattt gaataaggat tctccatttg attcaatcac ttctttatac
      121 caagtaaaag acattttctt atatcgattt aatgtaccac ttccatcatc gtttcgatca
      181 acataaatga gaccgtacct tttagaaagt tgtgcagtgg acatagaaac acagtcaata
      241 catccccaag acgtatagcc cataatttca acaccatcct gtagagcttc agcaacttgc
      301 aataaatgtt ctttcatata ctgaattcta taatcatctt ggacggttaa gttattaagt
      361 tcatctttta ttagttgatc tttagcacct aatccatttt ctactataaa taatgggatt
      421 tgataacggt cataatatct atttaaaatt atacgtagtc caattggatc aatttgccat
      481 ccccactctg aagactctaa ataaggattt actaaaccac caataatatt cccttctcct
      541 gaattatact gtgttggaag agcagattga gtcacactca tgtaatagct aaaggataaa
      601 aaatctacgg tataattttt taataactct gcatcttcag ctgcaaactc tatgttaatg
      661 tcattttcct taaaatatct ttttgcataa ttcggataat aacctctaac atgcacatct
      721 gaaaatagat aatttagatt ctcatactca tgagtcgccc atacatcttt tggatttgga
      781 gtcattggat aagctggcat agctaatacc atacatccca ccttaaactc tgaattaatc
      841 tcacgagcaa tttttgtaac caaacttgag gcgactaatt catgatgtat agcttgatat
      901 aattcttgtt tcgaaagatt ctccttaggt atatctattc ctccactagt aaatggtaat
      961 tccaaaacag agtttacttc gttaaatgta agccaatatt taactttatc tttatacctt
     1021 tctaaaactg ttcgagcaaa tttttcataa aaatgaatca ttctcctatc aacccatcca
     1081 tgatattttc ttgctaaata taatggagtc tcatagtgtg aaagagttac aagtggttct
     1141 atcccgtgag catgtagttc atcaaacaat tcatcataat atttcaaccc agcttcgtta
     1201 ggttcttcct catctccttt tggaaaaatt ctactccatg caatagaagt acgaaaaaca
     1261 ttaaagccca tttcagaaaa caaggatata tcttccttat atttatgata aaaatcaata
     1321 cctatcaatt ttaagttatc ttctgtagga ttttctgttg cttctcctaa tccacctttg
     1381 ggtaacacat cctgaactga taagccctta ccatcttcat tatatgctcc ctctacttga
     1441 ttagctgcaa cagctccacc ccaaagaaaa tcatctggaa aaatggtcat aactttcctc
     1501 cattataata ttaccagtaa ttccttagaa tgctcgattg tctgattatt aggtaatact
     1561 aatacatcta gaaaatcatt ggtattcgtt acaattactg gtgtaactgt ttcgtagcct
     1621 ttagtcttga ttaaattcaa gtccatttca aaaatcaact gatttttgaa aactctgtct
     1681 ccttcttcta catgactaat aaaaccttga ccttttagct caacagtatc taatccaata
     1741 tgaattagta actcaacacc ctcatcactc ttcaatccaa ttgcgtgctt agtcggaaaa
     1801 atatttgtaa ttttcccatc aaatggtgca taaaccttac cttcacttgg gataatcgct
     1861 actccgtctc caattagttt atctgaaaat gttttatcct ggacatcgct taacggaatg
     1921 atttctcctg atataggaga aaatatcatt tttttatttg aaactccagc ttcaacttct
     1981 aaattgctag aactctcttc ttcatcgatt ccaaatatat aagctaatac aaaggtaata
     2041 acaaccgaaa tgaccgccac aattaaagca tttacaatat ttgatggcac atcagaataa
     2101 ataaattgag gcaacgctat caaagatggg acagcaaata gatatgcttt aacactagta
     2161 agacctgcaa ataatcccgc taatccacca ccaatcatag ctgcataaag cggtttttta
     2221 tattttaaag tcacaccata taatgcaggt tcggtaatcc ctgcaagtaa ggctgagaaa
     2281 cctgctgcaa aagcaatttg ttttgtatta ttatttttac tctttaatgc aacagccatc
     2341 gaagcagccc cttgagctaa gtttgaccct aacattgctg gaagaattaa tacgtctgga
     2401 gtagcaatag atgccgccaa aaaaataggt gcaaaagccc aatgcattcc agtcataaca
     2461 ataaatggca taatagcacc aagaatagct aatgtaagcc atccagctac accatacatt
     2521 tgcccaacta gatttgataa tccttcacca acaattactc caataggtcc gactacaact
     2581 aaggcaatac agcttgatac taataatact agcgtaggtt gcaaaaaact cttagtaata
     2641 gctagtgtta atttagcaat tattttttca atatatttca tcaaccaaac cataataaga
     2701 attggaacga ctgatgaacc ataactagct ggtgtcacag gtgcaccaaa taaactaaga
     2761 ggattccctg attgcaccat ttgaacaaaa tttggatgga gaagtacacc tgctacagac
     2821 atagctaatg tagatgttac ttttaatttt tgtgatgcag aataagctaa taacagcggt
     2881 aagaaataat atggagcatc cccaaaaaat gtcaaaaaag caatagtctg agaatctgat
     2941 tgcaatatac caagcattgg taaaatgatt accaagactt tcaacatacc tccccctaac
     3001 attgctggaa tgattggagt catggaacca gcgatatact caatgattct ttctaaaata
     3061 ttccctttgt gcccttgaac aactgaatcg gattcaaaat tgccaagttt aacgaattct
     3121 ttataataat tagctacatc attaccaagt ataatttgat attgtccatt ctttttcata
     3181 atacctatta cacctggtat cttcttcaca tcatcatcat tgactaaatt ttcatctttt
     3241 aattctaatc ttaaacgtgt tacacaatgg gtaactctat tgacattttt ttcacctcca
     3301 attacatcga ggattttttg taccgtatct ttataactca tggtattctc ctattctatt
     3361 aatctaaatt ttttgttaag cgacgaatat gagccatcaa ataagctaat tcactagaag
     3421 tcagcaaata attgtactcc gtttgtataa acattgctac ctgttcacca cattcatatt
     3481 ctctaggata tttatttttc attaatgcta acaagtcttc atcatcatcg tcacaataag
     3541 ttgtattttc aaccaagcga tgggcaaata attttatatg agttatgaat ctataataat
     3601 caatagaatc ttggttaaac tcagtacaat agaaatcttg aatgattttc tcaattccca
     3661 taattatttc agtgatttta tacgactctt gaaaattatt ttctaaatta gcattaacaa
     3721 aatgtagcgc tatgaatgca gcttcgtcaa ttggaagttc aatacctaac tcttttttta
     3781 ttatttgtaa agcccttttc ccaagttcaa attcttcttt ataatatttc gatatttccc
     3841 agcgtagggg attcgaaata ataatccctt ctttataacg ttctatgctc gaagaaatat
     3901 gatccgttaa attaatatag ataatttcat caaaattatt acccaatgtt attttaccta
     3961 ggttaattat tttttcggtg atgtacacca actccaaagg aacgttaata aacatctctg
     4021 taaaacgggt catattatct ctatttttga gaaaaaaact tttctcaata tcaattggat
     4081 ttactttatc tccagttttt cttccaaaag ctattcccct tccaaaaaga agaatatcga
     4141 tatctttttt actttgcgca attacggcat tgtggtttag tattcgtttt atgatcataa
     4201 aatttccctt ttaaaataaa aggaacctac ttaaaaacat caagaaactt cttctgatgt
     4261 gattaaatag gttcctgctc acttgtatta gtgataacat ccttttattt caatttttaa
     4321 tggtgacat
//
LOCUS       DAVLXF010000116         4303 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLXF010000116 DAVLXF010000000
VERSION     DAVLXF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.116"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            529..885
                     /locus_tag="VYO12_001953"
     CDS             529..885
                     /locus_tag="VYO12_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588487.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
     gene            890..1255
                     /locus_tag="VYO12_001954"
     CDS             890..1255
                     /locus_tag="VYO12_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1588488.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLIPGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            1242..3065
                     /locus_tag="VYO12_001955"
     CDS             1242..3065
                     /locus_tag="VYO12_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1588489.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGIDFQK"
     gene            3179..4045
                     /locus_tag="VYO12_001956"
     CDS             3179..4045
                     /locus_tag="VYO12_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588490.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEEVNELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKFYMEWIKSLIDFYFYGQKEEAITRLENMLPQLNVSDLNYLQVSNTLFN
                     FYYDIEDLERFNEIRDKLEHQVNQLNLNTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKEYRTTYLLADLYLLMGSVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYFADITE"
BASE COUNT         1532 a          649 c          865 g         1257 t
ORIGIN      
        1 ttatcaattc caaagatgat ataacaatct tcgtgatgtg atgtattgac aaaatttaag
       61 atatcacgca ccaattcagt ttttgaatga tgccattttt gtttaaaatc atgaaactca
      121 tcttcgggtg tattcaatag taagtgtatt tgatctagac tcagcatttt tatttctcca
      181 aatattagtt tacagaaatt ttatcataga atcagagaaa gtcaatcata actatattag
      241 ctgtgatacc tttttcaaat cttccctgtt atcctaatat cattcataga aaatcaattt
      301 ttgattttct atgggggttt gggggcgaag tcaccaagtt atcttatcgt tggctgtcaa
      361 aactagaaag ttttgatagg ctggcgatat gatttttgag atattgtgga cacaatatct
      421 gagctcgcaa agccttacaa gaatgaaaat cagttgttgg aaaagtgtac tgacattgta
      481 tggtagctca cattgtcagt acaagtattt tggaaaggaa gtagcagtat gaaacgagat
      541 gtgcgtgata ttcggaaaca atttcgttta acagaagcag aagaaaagca aattctagct
      601 ttgatgagag agcggggaga gactaatttc tctgattttc ttcgtaaaag tttactttcc
      661 tctgatttac aaaaacagat ggagacatgg tttgccctct ggcaatccca aaaactagaa
      721 caaatcagtc gtgacgttca tgaagtttta atcttggcac agtcagaacg tcaagtcacc
      781 caagagcatg tatctattct cttaacgtgc gtgcaggaat tgattcaaga ggttgcaaac
      841 accatacccc tcagtaaaga atttcgtgag aagtacatga ggtaagcaca tggaacatcg
      901 ttaccgaacc aatctcaaga aagtgttttt gtctgatagt gagttgaacc aactaaatat
      961 aaatatcgat caaagtggtt gtaaatcctt ttctgaatat gcgagacgaa ctctactcga
     1021 tcctggtatg aattttatca cgattgacac aaacggttac caagatttag tgtttgagtt
     1081 aaagaggatt ggcaataata tcaaccagat tgctcgaagt gttaatcaat ctcagttaat
     1141 tcctggtgaa gaattgcagg agttgaaaaa aggaattggt gaattgataa aagaagttga
     1201 taaggaattt aatctgcaag cgcagaagct aaaggagttc catggtcatc actaaacact
     1261 ttgccattca cggaaagagt taccgcagaa agcttatcaa gtacattctc aatcctgaga
     1321 aaaccaataa tcttgccttg gtgtcggact atggcatgaa gaattttctg gactttccta
     1381 gctatgagga aatggtgcag atgtatcatg aaaatttcat cagcaacgat acgctttacg
     1441 attttcgcca cgacaggatg gaagaaaatc aacgaaaaat acacgctcac cacatcattc
     1501 agtctttctc gccagaggat catctcactc ctgaacaaat caatcggata ggttatgaga
     1561 ctgtgaagga attaactggt ggcaaatttc gttttatcgt tgcgacccat gttgataaag
     1621 accacctgca caatcacatc attatcaatt cagtagatag caattctgac aaaaagctca
     1681 agtgggacta caaggtggag cgaaatcttc gcatgatttc tgaccgtttt tctaaaatcg
     1741 caggtgctaa aatcattgag aaccgctatt ctcaccagcg gtatgaagtc tatcgtaaga
     1801 ctaatcacaa gtatgaactc aagcagcgac tctatttttt gatggaacat tctagggact
     1861 ttgaggattt caaaaagaat gctccgctac tacatgtgga gatggatttc cgtcacaagc
     1921 atgccacctt ttttattacg gactcaacta tgaaacaggt ggtgcgtggc aagcaactca
     1981 atcgcaagca gccttacaca gaagaatttt ttaagaacta ctttgccaaa agagaaatag
     2041 aaagtctcat ggaattttta ttgctgaaag ttgagaatat ggatgattta cttcagaaag
     2101 caaaactttt tggactaact atcaatccta aacaaaagca tgtttctttt caatttgcag
     2161 gagtggaggt aaaggagaca gagctagacc agaaaaatct ttatgatgta gagtttttcc
     2221 aagattattt taaaaataga aaagattggc aagctccaga aactgaggat ttcgttcaac
     2281 tttatcaaga agaaaagtta tccaaagaaa aagaacttcc aagcgatgag aagttctggg
     2341 agtcctatca agagttcaag agtaacagag atgccgttca tgaatttgag gtggagttgt
     2401 cactcaatca aattgaaaaa gtagtggatg atggaattta cgtcaaggtc aagtttggta
     2461 ttcgtcagga gggacttatc tttgtgccga acatgcagct tgatatggaa gaggataagg
     2521 tgaaggtttt catcagggaa accagctcct actatgtcta ccacaaagac gctgccgaga
     2581 aaaattgtta tatgaaaggt cgaaccttaa ttagacagtt cagctatgaa aatcaaacca
     2641 ttccattacg cagaaaagcg acagtcgata tgattaaaga gaagattgcg gaagtggatg
     2701 ctttgattga actggaagta gaaaatcaat cttatgtcac gattaaagat gagttagtgc
     2761 atgaactagc agcgtctgaa ttaagaatca atgagttgca agaacgaatg tcaaccttga
     2821 atcaagtagc agaatatcta ctggcttcag ttgaaagtaa gcaagaaatg aaattaaatc
     2881 tttcaaaact gaatataact gagaatatca gtgctaatat tgttgagaaa aaattgaaga
     2941 gcctggggaa tcaactggaa ttggaaaggg gcaggtatga aaagatggta gtcaggctag
     3001 ataaatttat aaatcgctta aatacaggac tttcaaaagg agatgggata gattttcaaa
     3061 aataggaatc tagccaataa ttatatacaa tgtgtatctt ttgtaaaata tatttgaaat
     3121 ttagatttaa tcatgttaca attaacattg aaatcaaata gaaaaggaag atgcaaacgt
     3181 gggtacacta ttagcaacaa gattaaaaaa tagacgaaaa gaattaaaaa tgtctcagcg
     3241 agaattagct gagggtatct gtaaacaggg gcaaattagt cgattagaga gtggagagtt
     3301 cacccctgga gcggattttt tacatgccct tgctaagaaa ttaaaagtta gtatggacta
     3361 cttttttgat gagcaggttg tagaggaagt caatgagtta tcagagttta agaagttagc
     3421 acagacattt atcacaaatc gtaattatga atctttaaaa tatatatatg aattggaaaa
     3481 tgtaaaagcc catcgcttat ctctagcaga taaattttac atggaatgga taaagtctct
     3541 tatagatttt tatttttatg gacagaaaga agaggcaatt acacgattag agaatatgct
     3601 acctcagtta aatgtatctg atttgaacta ccttcaagtt tcaaacactc tattcaattt
     3661 ttattatgat attgaagatt tagaacgttt taatgagatt agagacaagc tagagcatca
     3721 agtgaatcag cttaatttaa acacaattga agagttagaa ctatcaatta agttcaatta
     3781 taacgtttgt cgatacctat ggttacagaa aaatattgaa gaagctatta cgaaaatcac
     3841 agctacgata aagcaatgta aggagtacag gacaacttat ctattagctg atttgtattt
     3901 attgatggga agtgtaagtg aaaatttttc ttctaaaagt tcagtaaaag aatattttga
     3961 gactgctcat tttctctaca aacttgagga aaacatgtca atggctttga aagtcgaaca
     4021 ttattttgca gatataacag aataaggaat acggtgttct agtgagcatc gttttttaaa
     4081 tctaatttga atacaaaaaa gtattaaata aaaaaaaata aaaaaagatt ataaaaatac
     4141 ttgacaagct aattttgaag tgataaaata aatgcaagaa aacgaaagga gaggtatatg
     4201 agaagtttgt ttaggaaaat tgttgctttg ttagtcatag gattaatcct attagggact
     4261 gctggaggaa ctcaagttca taagatggct agagggattg atc
//
LOCUS       DAVLXF010000117         4301 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLXF010000117 DAVLXF010000000
VERSION     DAVLXF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.117"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     regulatory      complement(30..118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(327..956)
                     /locus_tag="VYO12_001957"
     CDS             complement(327..956)
                     /locus_tag="VYO12_001957"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1588491.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(958..1740)
                     /gene="thiM"
                     /locus_tag="VYO12_001958"
     CDS             complement(958..1740)
                     /gene="thiM"
                     /locus_tag="VYO12_001958"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1588492.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(1983..2651)
                     /locus_tag="VYO12_001959"
     CDS             complement(1983..2651)
                     /locus_tag="VYO12_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1588493.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYNV"
     regulatory      complement(2866..2954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3208..>4301)
                     /gene="lctO"
                     /locus_tag="VYO12_001960"
     CDS             complement(3208..>4301)
                     /gene="lctO"
                     /locus_tag="VYO12_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1588494.1"
                     /translation="NTYDLEPMAQQVIPKAAFGYIASGAEDTFTLRENIRAFNHKLIV
                     PHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVATARGVHEFGSLYTTS
                     SYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEGYKAIVLTADATVGGN
                     REVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSPRDVEFIAEYSGLPVY
                     VKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQEVAEAVDRRVPIVFDS
                     GVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEHLNAELKTVMQLSGAQ
                     TIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT         1302 a          956 c          690 g         1353 t
ORIGIN      
        1 aagattagac agttcttttc gacatacgaa aaaaacattt cacatttccc ttcgccagtc
       61 ttaactgtat caggttcaat gggtatcatc tcagcctaaa gcaccccaaa tgcctttatt
      121 atttaattat gtgattatta taacacacat tttatactag ttcaagaaat tgaactggaa
      181 atacagcctt gcactcacaa agacagcaga tctttctttt gcaaaaaaca aatgacctgt
      241 ttgatgaatt aaccattcaa gctgaatcta gacatagctt tttaaaaaag gaaaatccta
      301 cttacttaga atccaaggat agatatctat tgctcactca tttcccgaac agttttttct
      361 atattttttg catacgatat tgccgaaatg attgaaacgc cgtcaacatt ggtctttata
      421 atgtctttaa tatgtttcgt ctgtatccca ccaattgcaa ctaaaggcat ttgtggcaat
      481 agttttctca tcaattcaag accttcataa cctatagcac caccagcatc atcctttgac
      541 tgggtatcaa atacaggacc aacaccaaca taatctacat attcaacttt tgattgttga
      601 aattcttcct cgttttttat agaaagacca attattttat ctggcatcaa ttttctaatt
      661 tcatcaacac caatatcatc ttgacctaca tgtacgccat cagcgtcaat ttccattgcc
      721 aaatcgatat catcattaac gataaacggc acattgtatt ttttacaaag ttctttaatt
      781 tggatagcta gctcaagttt ttctaagcct tctaaagcac cctcaccttt ttctcgaaat
      841 tgaaataagg ttataccacc ttttaaggct tcctcaacga ctgtatatag attttttcct
      901 tggcaagtag tcgttccaca aataaaatat agttttagta attctttatg aaacatctta
      961 cttcactctt ttgaattcct ttacatcttc atctgtaatc tcgtataagg catttataaa
     1021 ttcaacttta aatgtcccag gaagatgtcc atttggacgt ttttctgcca tttctccagc
     1081 gatattgtaa accaacattg ctgtttctaa tgatttcaat tcttgacctt tttctagtcc
     1141 gataaagctt gctaccactg cccctaataa gcatcctgtc ccaatgactt tcggcatcat
     1201 agcactacca ttatgaatcg ttaccacttc tccattaaca gcaatggcat ccacttcacc
     1261 tgttactact attggaatat tgaacttctc atttgctgct agagcaattt cgtcaatatt
     1321 atctacgccc gcactatcta ctcctttaga tgccacatct attcctacta aagaggcaat
     1381 ctcgccagca tttcctctaa tcgctgctag tttataattg ttgattagat catctgctac
     1441 tttttttcta tattctcctg ctccacaggc tacaggatct aaaactgctg ggacattata
     1501 tttctctgca attttcagag cagcttggta taatttccaa ttttcatctg tcaatgttcc
     1561 tatgtttatt aataaaccac cagcatactt taacaaatcc tctaaatctg ctggaaactc
     1621 actcatggct ggtgaggcgc ccagtgctac taatccattt gctgtgaaat tttttactac
     1681 atcattggtt atacaaatga ccaatggtgc tttttctttt aataatttta aacttgtcat
     1741 attgaaatcc ttccttttca ctttatacga tctactgatt tcgatttatt tctatctttc
     1801 gttaagaatt tttttcattt acattgaatg atttatactc aatgaaaatc aaagagcaaa
     1861 ctaggaggct aaccgcaggt tgctcaaaac actgttttga ggttgtggat agaactgacg
     1921 tggtttgaag agattttcga agagtcttac ctcatcaaat ttgtaaatat catgagcctt
     1981 ctctagacat tgtaaccaat atcaaaaaaa gctaattcta aagcgactgc ttgattccag
     2041 cgttgctgaa gttctgtcaa atcttctcga tttttatcga cacgattgag ttcgtcaacc
     2101 agaaattgaa cccactctgc aaagaaagga cctctgtgga gattgatcca ttccgaatga
     2161 atatagactt caggtaaagc caaatcttta gaaccccagt ctaaatagag accttctgca
     2221 atgaccagca tgaccaaaag atgggcatag tctgatgaag ccaccgccga atacattaga
     2281 tcctgaaagg cttttgttac agggtgcaaa gtcacttcta gatagtcatt ctctgctact
     2341 tttaactctt taaaagcctt ttggaaataa ccatcttcat ctgcttcaag aaagcctagt
     2401 tgcttggcaa aacgaagctt ggattcaagt ttatctgcgt gagctacgca ggcacccagc
     2461 atggataaga aggcatcaaa gaagtgataa tcttgaatca gatagtcctt taagacctta
     2521 ttctcaattg tccccgcaaa aagttcctta acaaaacgat gattgattgc agcctgccaa
     2581 tccttctgac tgctttttaa taattctcca acagtcaaac ctggctgaaa tgcatagtct
     2641 tgtgtttcca tatttacttc tcctctcttt acttgttagt aattaataaa acaccaagaa
     2701 atatcaagca aaatcgtaat tccacttgat ccttttaaag cacatcgaga gcatttgcag
     2761 agagctaact aaacaagcct atccagttta tataaacaaa aaactccaat tacaatcaag
     2821 aattagagtt gacttacaag attagatcgt tcatttcgcc atacgaaaaa acgtttcaca
     2881 tttcccttcg ccagtcttaa ctgtatcagg ttcaatgggt attatctcag cctaaagcac
     2941 cccaaatgtc tctattattt aattactgaa ccagtatagc aaaaaatgaa agccctagca
     3001 agatatttga ccgaaaaata tctttatata taatatattg aaactagaat agtacacctc
     3061 tacttctaaa acattgttag aaatcgattt gactgtcctg attgatttgt cctattctta
     3121 tttcatttta ctatagtttt cgatagcaat ttattcttcc aatacacgaa gaaaaacctc
     3181 cacattcagt ggaggcaatc tgttttatca atacaatttt aagtcacgag ggtcaactgg
     3241 gaaggttggg ttgtatggat tgtgacggag cttaaagtgt ttgacatctt caatggtctg
     3301 agctccagac aattgcataa ctgtcttcaa ttccgcattc aagtgttcaa agacttgacg
     3361 cacaccgaca ctaccaccga gagccaagcc ataaataaca gggcgaccaa tagctaccaa
     3421 gtctgctcct gaagccaagg ctttaaagac gtgttgacca cgacgaacac cagagtcaaa
     3481 gacaatcggc acacgtctat caactgcttc tgccacttct tgaagcgagt caaaggcagc
     3541 tggtccaccg tcgatttgac gaccaccgtg gttggttacc cagataccag aagctcctgc
     3601 agcaagtgaa cgttcaacgt cctcacggca ttgtggtccc ttgacataca caggaagacc
     3661 agagtattca gcgataaatt ctacatcgcg tggagacaag cgttgtttag ctgatttgta
     3721 aacaaagtcc attgatttac cagcaccttc tggcaggtat tcttcaacaa tcggcatgcc
     3781 aactgggaag acaaaaccat tacgcttatc cacttcacga ttccccccta cagtagcatc
     3841 tgccgtcaag acaatcgctt tataaccttc agccttcaca cggtccatga tgtggcggtt
     3901 gataccgtca tccttactaa agtaaaattg gaaccaatgg ggtgtccctt gaagggcttc
     3961 agaaatctct ggaaggtcaa cagtagagta agagctggtt gtataaagag aaccaaactc
     4021 atgcacacca cgcgcagtcg ccacttcccc ctgttcattt gccaatttat gagccgcaac
     4081 aggcgccata atgattggtg aagatagttt ttcacctgca aattcaatct ctgtacttgg
     4141 attttcaaca tcgcaaagtg tatgaggaac gatgagtttg tggttaaagg cgcggatatt
     4201 ctctcttaaa gtgaaagtat cttccgcccc actagcgata tagccaaatg ctgctttagg
     4261 aataacttgt tgcgccattg gctccaaatc ataggtattg a
//
LOCUS       DAVLXF010000118         4196 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLXF010000118 DAVLXF010000000
VERSION     DAVLXF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.118"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            229..2730
                     /locus_tag="VYO12_001961"
     CDS             229..2730
                     /locus_tag="VYO12_001961"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1588495.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDTSKPKFYALDMFPYPS
                     GVGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDISREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            3030..3722
                     /locus_tag="VYO12_001962"
                     /pseudo
     CDS             3030..3722
                     /locus_tag="VYO12_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            3734..4150
                     /locus_tag="VYO12_001963"
                     /pseudo
     CDS             3734..4150
                     /locus_tag="VYO12_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1259 a          797 c          969 g         1171 t
ORIGIN      
        1 agcattccag ttttatcttc ggtctaaaat aagtattttg tgctatacga gataagcgtc
       61 ttgacttact ccttgattta ctgcataaca atgggataaa aagtgggaga tagagcaatt
      121 catagtcatc aaaattaatg agatacagta tacagttttt tctttaaaca catttcaaat
      181 tccctcaaaa atggtataat agtaacatca caaaattgga gagagaccat gagtttttat
      241 aatcataaag aaattgagcc taagtggcag ggctactggg cagaacatca tacatttaag
      301 acaggaacag atacatcaaa acctaagttt tatgcgcttg atatgttccc ttatccgtct
      361 ggagttggtc tgcacgtagg acacccagaa ggttatactg caaccgatat cctcagtcgt
      421 tacaaacgtg cgcaaggcta caatgtcctt cacccaatgg gttgggatgc ttttggtttg
      481 cctgcagagc aatacgctat ggatactggt aatgacccag cagaatttac agcggaaaac
      541 attgccaact tcaaacgtca aatcaatgcg cttggatttt cttatgactg ggatcgtgaa
      601 gtcaacacaa cagatccaaa ctactacaag tggactcaat ggattttcac caagctttac
      661 gaaaaaggct tggcctatga agctgaagtg ccagtaaact gggttgagga attgggaact
      721 gccattgcca atgaagaagt gcttcctgac ggaacttctg agcgtggagg ctatccagtt
      781 gtccgcaaac caatgcgcca atggatgctc aaaatcacgg cttacgcaga gcgcttgctc
      841 aatgacttag atgaactaga ttggtcagag tctatcaagg atatgcaacg caactggatt
      901 ggtaaatcaa ctggtgccaa tgtaactttc aaagtaaaag gaacagacaa ggaatttaca
      961 gtctttacta ctcgtccgga cacacttttc ggtgcgactt tcactgtctt ggctcctgaa
     1021 catgaattag tagacgctat cacaagttca gagcaagcag aagctgtagc agactataaa
     1081 caccaagcca gccttaagtc tgacttggtt cgtacagacc ttgctaagga aaaaactggt
     1141 gtttggacag gtgcctatgc catcaaccct gtcaatggta aggaaatgcc aatctggatt
     1201 gcggactatg ttcttgctag ttatggaaca ggtgcggtta tggctgtgcc tgcccacgac
     1261 caacgtgact gggaatttgc caaacaattt gaccttccaa tcgtcgaagt acttgaaggt
     1321 ggaaatgtcg aagaagctgc ctacacagag gatggcctgc atgtcaattc agacttccta
     1381 gatggattga ataaagaaga cgctattgcc aagattgtgg cttgcttgga agaaaaaggc
     1441 tgtggtcagg agaaggttac ctaccgcctc cgcgactggc tctttagccg tcaacgttac
     1501 tggggtgagc caattccaat cattcattgg gaagatggaa cttcaacagc tgttcctgaa
     1561 actgaattgc cgcttgtctt gcctgtaacc aaggatatcc gtccttcagg tactggtgaa
     1621 agtccactag ctaacttgac agattggctt gaagtgactc gtgaagatgg tgtcaaaggt
     1681 cgtcgtgaaa ctaacaccat gccacaatgg gctggttcaa gctggtacta cctccgctat
     1741 attgacccgc acaatactga gaaattggct gatgaggacc tcctcaaaca atggttgcca
     1801 gtagatatct acgtgggtgg tgcggaacat gctgtacttc acttgcttta tgctcgtttc
     1861 tggcataaat tcctctatga cctcggtgtt gttccgacta aggaaccatt ccaaaaactc
     1921 tttaaccaag ggatgatttt gggaacaagc taccgtgacc accgtggtgc tcttgtggca
     1981 accgacaagg ttgaaaaacg tgatggttcc ttctttcatg tagaaacagg ggaagagttg
     2041 gagcaagcgc cagccaagat gtctaaatcg ctcaagaacg ttgttaaccc agacgatgtg
     2101 gtggaacaat acggtgccga tacccttcgt gtttatgaaa tgtttatggg accactcgat
     2161 gcttcgattg cttggtcaga agaaggtttg gaaggaagcc gtaagttcct tgaccgagtt
     2221 taccgtttga ttacaagtaa agaaatcctt gcggaaaaca atggtgctct tgacaaggtt
     2281 tacaacgaaa cagtcaaagc tgttactgag caaattgagt ctctcaaatt caacacagct
     2341 attgcccaac ttatggtctt tgtcaatgct gctaacaagg aagataagct ttatgttgac
     2401 tatgccaaag gctttattca attgattgca ccatttgcac ctcacttggc agaagaactc
     2461 tggcaaacag tcgcagaaac aggtgagtca atctcttatg tagcttggcc aacttgggac
     2521 gaaagcaaat tggttgaaga tgaaattgaa attgttgtcc aaatcaaagg aaaagtccgt
     2581 gccaaactca tggttgctaa agatatatca cgtgaagaat tacaagaaat cgctttagct
     2641 gatgaaaaag tcaaagcaga aattgacggt aaggaaatcg tgaaagtaat tgcggtaccg
     2701 aataaactcg ttaatatcgt cgttaaataa cgagtttatt agctctatct gccaccttca
     2761 atagtccact ggactattga agccaactaa attagttaac attgttgtga aataagatag
     2821 gaatccttca gagtagaatc tggaggattt tttgaacctt cttatgaaag tatgatatat
     2881 agtagactga atctaaaata gtacgaaaca attgctaaaa catttataga aattaatttt
     2941 actttcccaa tcgatttgtt ctcatcttat ttcaatacgc tatactatgg gcaactataa
     3001 agtttgaaaa gcgaaacaag gagaaagtta taccagtaaa cgaatatggt cagatgattg
     3061 gtgagtcaat ggaaggttat acaccaggtg aactgacttt tattgatctc ttagaagggc
     3121 gttatgctag gatagaggct ctttcagtgg aaaagcatgc ggaggatttg ctagctgttt
     3181 atggtcctga tactcctcag gagatgtgga cctacctctt tcaggagcca gtagcagaca
     3241 ttgaggaact ggtcagcctt ttaaatcaga tgttggtcgt aaggaccgtt tttactatgc
     3301 aatcatagac aaggcaactg gtaaggcttt gggaactttt tctctcatgc gtattgacca
     3361 gtaacggagt aatagaagtg ggagctgtca ctttttctcc aaaactcagg gggacacgga
     3421 tagggacaga agcccagtat ctactggctc gctatgtctt tgaagagctt aactatcgtc
     3481 gttatgagtg gaaatgcgat gctcttaacc tgccatccag acgtgctgcg gagcgtttgg
     3541 gctttattta tgaaggaacc ttccgtcagg cagtggttta taaggggcgt acgagagata
     3601 tggattggtt gtctatgatt gataaggact ggcccaaagt caaagatcgt ttggaaacat
     3661 ggttgcgtcc tgaaaatttt gataaaaatg gacagcagta caagagcttg agagagtttt
     3721 aagaggtgtt gacatgatca ctattagaaa gcaagaaatc gtcaagttcg aggatgtttt
     3781 gcatctctat caggctgtcg gttggacaaa ttatacaaat caacctcaga tgctggagta
     3841 gaccttgtat cattcattaa taatttatct ggcacttgat ggtgatgctg ttgtgggctt
     3901 gattcgtttg gttggagatg gtttttcatc agtttttgta caggatttga ttgttttgtc
     3961 tagctatcag cgccaaggga ttggtagctc cttgatgaaa caggctttag gaaattttaa
     4021 agaggcctat caagtccagc tggcgacaga acagacagaa aaaaacgtgg gatttaatcg
     4081 ttctatgggc tttgaaatct tatccaccta taattggata gggatgactt ggatgaatcg
     4141 taaaaaataa caaaacttgt tttttcttaa gcaaaattta aggatggtct agtatt
//
LOCUS       DAVLXF010000119         4111 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLXF010000119 DAVLXF010000000
VERSION     DAVLXF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.119"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            696..1688
                     /locus_tag="VYO12_001964"
     CDS             696..1688
                     /locus_tag="VYO12_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1588496.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1690..2508
                     /locus_tag="VYO12_001965"
     CDS             1690..2508
                     /locus_tag="VYO12_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1588497.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2510..3295
                     /locus_tag="VYO12_001966"
     CDS             2510..3295
                     /locus_tag="VYO12_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1588498.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3343..3561
                     /locus_tag="VYO12_001967"
     CDS             3343..3561
                     /locus_tag="VYO12_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1588499.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3546..3743
                     /locus_tag="VYO12_001968"
     CDS             3546..3743
                     /locus_tag="VYO12_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1588500.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
BASE COUNT         1131 a          713 c          892 g         1375 t
ORIGIN      
        1 tcatcagaat gataaaatta atcaattgat atctgattac aaacagaata tgaaagcttt
       61 ttatatcact attgaaaaat ttatacgaga tgatgaaagc cttaagtgtt attttataaa
      121 ggttatttca agtcgttcca aggtaacaag tctagatcag attgaagctg ataaaacgat
      181 acaaagaaaa tattcaagtg agctaaaaaa atttattgga ttttataatg agattatttg
      241 tgaggaaaat agtttcctac atgtacgaaa gaggtggtcg agttggttta ggtagtcgat
      301 gcgtgagttg ataattctca gggtatggac ttctttttca tgaatgaggt aaaagagcag
      361 gtattgttta gagacaatca ttctgagcat attttctgga tagagggagt atccgatttt
      421 atgatcaaag ttaataccgc cctctggtga gaagatgagt aggttggtaa tttaaactat
      481 taaacagaat ttttgattaa aagtattatt ttatgagaga aatcctaatt tcacaatcca
      541 taggcaaact cttgcatttc gttttttatt ggactataat aggttggtat aaagcctttt
      601 gtagtaataa aatgtagaag gtgtagaaag caaggattta gaatatttgt agttaaaaac
      661 acaatgttgc tattccttac gatagggaga tagatatggc aatgatagaa gtggaacatc
      721 ttcagaaaaa ttttgtgaag actgttaagg aaccgggctt gaagggggct ttgcgctcct
      781 ttattcatcc tgaaaagcag acctttgaag cggtcaagga tttgaccttt gaggttccaa
      841 aagggcagat tttaggattt atcggggcaa atggtgctgg gaagtcgaca accattaaaa
      901 tgctgacagg aattttgaaa ccaacatctg gtttttgtcg gattaacggc aagattcccc
      961 aggacaatcg gcaagattat gtcaaagata ttggcgtagt ctttggacaa cgcacccagc
     1021 tatggtggga tttggctctg caagagacct acactgtctt aaaagagatt tatgatgtgc
     1081 caaactcgct ctttcataag cgcatggact ttttgaatga agtcttggat ttgaaggact
     1141 ttatcaagga tcccgtgcgg actctttcac tgggacaacg gatgcgggcg gatattgcgg
     1201 cctccttgct ccacaatccc aaggttcttt ttttagatga gccgaccatt ggtttggacg
     1261 tttcggttaa ggataatatt cgtcgggcaa ttactcagat caatcaagag gaagaaacta
     1321 ccattctttt gaccactcac gatttgagtg atattgagca actttgtgat cggattttca
     1381 tgattgacaa ggggcaagag atttttgatg gaacggtgag ccaactcaag gagacctttg
     1441 gtaagatgaa gactctctct tttgaactgc taccaggtca aagtcatctc gtctctcact
     1501 atgaaggtct gtctgatatg accattgata gacaaggaaa cagcctcaac attgaatttg
     1561 atagttctcg ctaccagtca gctgacatta tcaagcaaac cctgtctgat tttgaaatcc
     1621 gcgatttgaa gatggtggat acggatattg aggatattat ccgtcgcttc taccgaaagg
     1681 agctctagga tgatcaaatt gtggagacgt tataaaccct ttatcaatgc aggggttcag
     1741 gagttgatta cttaccgagt caactttatt ctctatcgga ttggcgatgt catgggggct
     1801 tttgtggcct tttatctctg gaaggctgtc tttgattctt cgcaagagtc tttgattcag
     1861 ggcttcagta tggcggatat caccctctac atcatcatga gttttgtgac caatcttctg
     1921 actagatccg attcgtcctt tatgattggg gaggaggtca aggatggctc cattatcatg
     1981 cgtttgttgc gaccagtgca ttttgcggcc tcctatcttt tcaccgagct tggttccaag
     2041 tggttgattt ttatcagcgt tggccttcca tttttaagtg tcattgtctt gatgaaaatc
     2101 atatcgggtc aaggtattgt agaggtgcta ggattaactg tcctttatct ttttagctta
     2161 acgctcgcct atctgattaa ctttttcttt aatatttgct ttggattttc agcctttgtg
     2221 tttaaaaatc tttggggttc caacctactt aagacttcca tagtggcttt tatgtcgggg
     2281 agtttgattc ccttggcatt ctttccaaag gttgtttcag atattctttc ctttttgcct
     2341 ttttcatcct tgatttatac tccagttatg atcattgttg gaaaatacga tgccagtcag
     2401 attcttcagg cactcctttt gcagttcttc tggctcttag tgatggtggg attgtctcag
     2461 ttgatttgga aacgggtcca gtcctttatc accattcaag gaggttagta tgaaaaaata
     2521 tcaacgaatg catctgattt ttatcagaca atacatcaaa caaatcatgg aatataaggt
     2581 ggattttgtg gttggtgtct tgggagtctt tctggctcaa ggcttgaatc tcttgtttct
     2641 caatgtcatc tttcaacata ttccattcct agaaggctgg acctttcaag agatagcttt
     2701 catttatgga ttttccttga ttcccaaggg aatggaccat ctcttttttg acaatctctg
     2761 ggcactaggg caacgcctag tccgaaaagg ggagtttgac aagtatttga ctcgtcccat
     2821 caatcctctc tttcacatcc tagttgaaac ctttcagatt gatgccttgg gtgaactctt
     2881 agtcggtggt attttattgg gaacaacagt gaccagcatt gtttggactc ttccaaaatt
     2941 cctgcttttc ctagtttgta ttccttttgc gaccttgatt tatacttctc ttaaaatcgc
     3001 aacagccagt atcgcctttt ggactaagca gtcaggcgcc atgatttaca tcttctatat
     3061 gttcaatgac tttgctaagt atccgatttc tatttacaat tctcttcttc gttggttgat
     3121 tagctttatc gtgcctttcg cctttacagc ctactatcca gctagctatt tcttacagga
     3181 aaaggatgtg ttctttaacg taggaggttt gatgttgatt tctctggttt tctttgttat
     3241 ttcccttaaa ctttgggata agggcttaga ttcctacgaa agtgcgggtt cgtaaaagct
     3301 aaagtaagac taaaatcaag aaaggcagtt gtcgctgagg ttttgaagga tttgccagaa
     3361 tggtttggaa tcccagaaag cacacaagcc tatatagaag gaaccacgac actgcaagtt
     3421 tggaccgcct atcaggagag tgatttgact ggatttgtaa ggttatccta ttcgagtgaa
     3481 gattgtgcag agattgattg tctcggcgta aaaaagctta tcaaggtaga aaaattggga
     3541 gccaattgct tgctacttta gagagtgaag ctcgtaaaaa agttggttat ctgcaggtca
     3601 aaacagtggc agaagcttct aataaagatt atgatcgaac aaatgacttt tatcgaggtc
     3661 ttggctttaa aaagttagag attttttctc agctatggaa tccgcaaaat ccttgtcaga
     3721 ttttgattaa aaagcttgaa taatattact tgacatctat tctcagagtg ccatactgta
     3781 agtgtaatcg ccgatttagc tcagttggta gagcaaggca ctcgtaaagc ctaggttata
     3841 ggtagataaa cgactgagga gttgaaaaaa tagataggta gaagataacc gttaagcctt
     3901 attcttagcg gttatttata ttatttaata gcgctgatat ttcatcaatt atgcctgttt
     3961 tcgtgtttct ggtagttgtt caagtttatt gctactattt ttgatggtat gaatgtgctt
     4021 ataatgtatc ccggttaacg aaagttttgg acttatactc ttcgaaaatc tcttcaaacc
     4081 acgtcaacgt cgccttgccg tgcgtatggt t
//
LOCUS       DAVLXF010000120         4054 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLXF010000120 DAVLXF010000000
VERSION     DAVLXF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.120"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..>4054
                     /locus_tag="VYO12_001969"
     CDS             1..>4054
                     /locus_tag="VYO12_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1588501.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVSDKG
                     VPEVQPELPEAVVSDKGVPEVQPELPEAVVSDKGEPEQVAPLPEYKGNIEQVKPETPV
                     EKTKEQGPEKTEEVPVKPTEETPVNPNEGTTEGTSIQGAENPVQPAEESTTNSEKVSP
                     DTSSENTGEVSNKPSDSTTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSN
                     QETEKPVQPAEETQTNSGKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSG
                     NTTSENGQTEPEPSNGNSTEDVSTKSNTSNSNGNEEIKQENELDPDKKVEDPEKTLEL
                     RNVSDLELYSLSNGTYKQHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKND
                     KILYKITAKVEKLQQEIESRYKDNFPFYLAKKGTEETTNFTSFSNLVKAINQNLSGTY
                     HLGASLNANEVELSTDDKSYIKGTFTGQLIGEKDGKHYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDSYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DRDAQIQDSYAEGDINNVKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKNEVVSKESFEERGTMLDASQIASKKAEI
                     NLITPPIVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKATIVKYGNLVK
                     ENSILYQKELLSAVMMKDDQVITDIISNKQTANKLLLHYKDHSSEKFDLRYQADFANL
                     AEYSIGDSGLLYTPNQFLYHQDSIINQVLPELNRVNYQSDAVRNTLGISPEVKLTELY
                     LEEQFTKTKEHLAENLKKLLSSDAGLVTDNE"
BASE COUNT         1547 a          709 c          806 g          992 t
ORIGIN      
        1 atggaaaagt attttggtga aaaacaagag cgtttttcat ttagaaaatt atcagtagga
       61 cttgtatctg caacgatttc aagtttattt tttatgtctg tattagctag ttcatctgtg
      121 gatgctcaag aaactgcggg agttcactat aaatatgtgg cagattcaga gctatcatca
      181 gaagaaaaga agcagcttgt ctatgatatt ccgacatacg tggagaatga tgatgaaact
      241 tattatcttg tttataagtt aaattctcaa aatcaactgg cggaattacc aaatactgga
      301 agcaagaatg agaggcaagc cctagttgct ggtgctagct tagctgctct gggaatttta
      361 atttttgctg tttccaagaa aaaggttaag aataaaacgg tattacattt agtattggtt
      421 gcgggaatag gaaatggtgt cttagtttca gtccatgctt tagaaaatca tcttttgcta
      481 aattacaata cggactatga attgacctct ggagaaaaat tacctcttcc taaagagatt
      541 tcaggttaca cttatattgg atatatcaaa gagggaaaaa cgacttctga ttttgaagta
      601 agtaatcaag aaaaatcagc agccactcct acaaaacaac aaaaggtgga ttataatgtt
      661 acaccaaatt ttgtagacca tccatcaaca gtacaagcta ttcaggaaca aacacctgtt
      721 tcttcaacta agccgacaga agttcaagta gttgaaaaac ctttctctac tgaattaatc
      781 aatccaagaa aagaagagaa acaatcttca gattctcaag aacaattagc cgaacataag
      841 aatctagaaa cgaagaaaga ggagaagatt tctccaaaag aaaagactgg ggtaaataca
      901 ttaaatccac aggatgaagt tttatcaggt caattgaaca aacctgaact cttatatcgt
      961 gaggaaacta tagagacaaa aatagatttt caagaagaaa ttcaagaaaa tcctgattta
     1021 gctgaaggaa ctgtaagagt aaaacaagaa ggtaaattag gtaagaaagt tgaaatcgtc
     1081 agaatattct ctgtaaacaa ggaagaagtt tcgcgagaaa ttgtttcaac ttcaacgact
     1141 gcgcctagtc caagaatagt cgaaaaaggt actaaaaaaa ctcaagttat aaaggaacaa
     1201 cctgagactg gtgtagaaca taaggacgta cagtctggag ctattgttga acccgcaatt
     1261 cagcctgagt tgcccgaagc tgtagtaagt gacaaaggcg aaccagaagt tcaacctaca
     1321 ttacccgaag cagttgtaag cgataaaggc gtaccagaag ttcaacctga attacccgaa
     1381 gcagttgtaa gcgataaagg cgtaccagaa gttcagcctg aattacccga agcagttgta
     1441 agtgataaag gtgaaccaga gcaggtagca ccattgccag aatataaggg gaatattgag
     1501 caagtaaaac ctgaaactcc ggttgagaag accaaagaac agggtccaga aaaaacggaa
     1561 gaagttccag taaaaccaac agaagaaaca ccagtaaatc caaatgaagg tactacagaa
     1621 ggaacctcaa ttcaaggagc agaaaatcca gttcaacctg cagaagaatc aacaacgaat
     1681 tcagagaaag tatcaccaga tacatctagc gaaaatactg gggaagtatc caataaacct
     1741 agtgattcga caacctcagt tggagaatca aataaaccag aacataatga ctctaaaaat
     1801 gaaaattcag aaaaaactgt agaagaagtt ccggtaaatc caaatgaagg cacagtagaa
     1861 gggacctcaa atcaagaaac agaaaaacca gttcaacctg cagaagaaac acaaacaaac
     1921 tctgggaaaa tagctaacga aaatactgga gaagtatcca ataaacctag tgattcaaaa
     1981 ccaccagttg aagaatcaaa tcaaccagaa aaaaacggaa ctgcaacaaa accagaaaat
     2041 tcaggtaata caacatcaga gaatggacaa acagaaccag aaccatcaaa cggaaattca
     2101 actgaggatg tttcaaccaa atcaaacaca tccaattcaa atggaaacga agaaattaaa
     2161 caagaaaatg aactagaccc cgataaaaag gtagaagatc cagagaaaac acttgaatta
     2221 agaaatgttt ccgacctaga gttatacagt ttgtcaaatg gtacttataa acaacacatt
     2281 tcgttagagc aagttccaag caatccaaat agctactttg ttaaagtgaa atcttcttca
     2341 ttcaaagatg tatacctacc agtagcatca atatcagagg aaagaaaaaa tgataaaatc
     2401 ctttataaaa tcacagcaaa agtagagaag cttcagcagg agatagaaag cagatataaa
     2461 gataattttc ccttctatct agctaagaag ggaacagaag aaacaacaaa ctttacttcc
     2521 tttagtaatc tggtcaaagc tataaaccaa aatctctctg gaacctatca tttaggagct
     2581 agcctgaatg ctaacgaagt ggagcttagt actgatgata aatcctatat caagggaacc
     2641 tttactggtc agttgattgg tgaaaaagat ggtaagcatt atgctattta caacttgaaa
     2701 aagcctcttt ttgaaaactt gagtggtgct acagtagaaa aactgagtct aaaaaatgtt
     2761 gctatttcag ggaaaaatga tattggttca ctggcaaatg aagctacgaa tggcacaaag
     2821 attaaacaag ttcatgttga tggtgttctg gctggagaac gtggtgtcgg tggtttgttg
     2881 gctaaggcag accaatcaag catcgcagag agcagtttca agggaagaat tgtcaatacc
     2941 tatgaaacga ctgattccta caatattggc ggtctggtcg gtcatttaac aggaaaaaat
     3001 gcgtctattg ctaaatccaa agcgacagta accatttcgt caaacaccaa taggtcagat
     3061 cagactgtcg gtggtcttgc aggtctagta gaccgagatg cacagatcca agatagctat
     3121 gctgaaggtg atatcaacaa tgtcaagcac tttggtagag tcgctggagt ggcaggcaat
     3181 ttgtgggatc gaacttctgg tgatgttagg catgctggaa gtttgaccaa tgttctcagc
     3241 gatgttaatg taacaaacgg aaatgccatc actggatacc actatacagg aatgaaggta
     3301 gctaatacat ttagtagtaa ggctaataga gttttcaatg tcactttaga gaagaatgag
     3361 gtcgtcagca aagaatcctt cgaggaaaga ggaacaatgc tagatgcttc tcaaattgca
     3421 agcaaaaaag cagaaatcaa tcttatcact ccaccaatag tggaacccct ctcaacaagc
     3481 ggtaagaaag acagtgattt ttctaagata gcccattatc aagccaaccg tgctttggtt
     3541 tataagaaca ttgaaaaatt gttacctttc tataacaagg cgaccatcgt gaaatacgga
     3601 aacctggtta aggagaacag tatcttatat caaaaagaac tcttgtctgc agttatgatg
     3661 aaggatgacc aagtaatcac agatattatt tccaacaaac agactgcaaa caaactcttg
     3721 cttcactaca aggaccattc atctgaaaag tttgatctca ggtaccaggc tgattttgcc
     3781 aatttggcag aatatagcat tggagatagt gggcttctct atacgccaaa tcaattcttg
     3841 tatcatcaag attctattat taaccaagtg ctgcctgaat tgaatcgagt aaattatcaa
     3901 tcagatgctg ttagaaatac acttggaatt tcaccagaag tgaagctaac ggagttatat
     3961 cttgaagaac aatttaccaa aacgaaagag catctagcag agaacttgaa aaaactttta
     4021 tcatctgatg caggtttagt aacagacaat gaag
//
LOCUS       DAVLXF010000121         4023 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLXF010000121 DAVLXF010000000
VERSION     DAVLXF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.121"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..1557)
                     /locus_tag="VYO12_001970"
     CDS             complement(<1..1557)
                     /locus_tag="VYO12_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1588502.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRENLSNLRTYRRQNSDNTPRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEERIARIIPLRYRSNHWVPDSRPEEPSPQPAPNPQPAPSNPIDEKLVKE
                     AVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSD
                     REFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVPSDKVKLVEDILAFLAP
                     IRHPERLGKPN"
     gene            complement(1811..3544)
                     /gene="ptsP"
                     /locus_tag="VYO12_001971"
     CDS             complement(1811..3544)
                     /gene="ptsP"
                     /locus_tag="VYO12_001971"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1588503.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(3550..3813)
                     /locus_tag="VYO12_001972"
     CDS             complement(3550..3813)
                     /locus_tag="VYO12_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1588504.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT         1043 a          863 c          789 g         1328 t
ORIGIN      
        1 atttggtttt cctaaacgtt ctggatgacg aatcggagct aagaaggcaa gaatatcttc
       61 cactaacttg actttatcac ttgggacatc cttgagtcgt tccaacaggt tatccaaagc
      121 ctcaaaatca acttgtcgac ctttattatc aagtaaatct tggtgaattc ttgctagtaa
      181 gtcataagcc ttattgtaaa attctcgatc actagatggg aggtcagttt tcttagctcc
      241 tagcttatga gataaacttt cctgcttggc cagtttgcta tcaatgcctg ctgctgtttc
      301 tgctgaaaga tccttggctg ggatataacg agaaactcca ttctcctcaa agacataacc
      361 atcgcctact tttcgaacag cttctttgac caatttctca tcaattggat tgcttggagc
      421 tggttgagga tttggtgcag gttgtggact tggttcttct ggtcttgaat ctggtaccca
      481 atggtttgaa cgataacgaa ggggaataat acgagcaatt cgttcttcca attcagacat
      541 ttgttcataa gggataaagt ggtaatggtt accatgaggg acagctacac ctctggcggt
      601 tcgacttgtg atttgcgctg ggtcgaaaat aaggccatca gattctacat ggcgttgact
      661 caaaggcagt ttgtagagct gtttcaagag actatcaatg tcattacttt gacttgcttg
      721 actgttagtg ttgctgttgt tgcttgtgtt agtatttgta gttcctggat tgcttacaga
      781 aggtacccag tttgttcttg gagtgttatc gctattttgt cggcgatagg ttcttaaatt
      841 tgacagattt tcccgaccag ataggaaggc ttctgcagca gccaactcgc tagctgataa
      901 ctcattctta ggaatgtaat ggtaatggtc gccgtgagga acgatataag catcacccgt
      961 gtcctcaatg atatcagatg cattgaagat atacccatca tctgtggtgt agcgtccctg
     1021 tgaacgtgca aaggctaccg caccatcgtt tgctgaagtc cctccttcac gatgctgact
     1081 atgttcttgt ttttgccgat tgatttcttc ttttgtacgg acattatccg cgtgggcagc
     1141 atccttaagg taaacatagt attttccatc taccttgata acataaccac ccttgatttc
     1201 actgataata tcctcgtctt ttagctggta gtttggatcc ttcatcagca attcttcact
     1261 gatgatagcg tcataaggaa ccttcccatt gtaataatga tagtggtcgc catgtgaagt
     1321 cacatagcct tggtctgtta tcttgatgac gatttgctca gcattgattc cttcacgctt
     1381 gctaacctca tcaggagtca aattctccgt tttttgcgtc gcttgttttc catctatata
     1441 ggaaacacga ttattttcct taaccgttct agcttgatac agtcccaact cgtaagaaca
     1501 aacacttaaa atcaaagctg ccgcagaacc aacaaggtat ttcttattaa ttttcatcga
     1561 aactctcttt ctatttgata tttctagttg caattcattt ctaaattgca gaaactcctc
     1621 tctattactt aactggttaa tagtttactc ctaaatctcc aacttgtcaa gaattttatg
     1681 aaccatttaa gtattttttg ttctgaccac tactttttca aggttggact ttctataaga
     1741 aagattttct tgaaaattct ttttaaaata tcaaaaaaat ccctgtaact gagttgcagg
     1801 gatttttcga ttaatcaaaa ttaacgtatt ctttttgaag ttcaagaact tcttccattg
     1861 ttgagcattc tgtaagggca cggtttgcgt actcttccat cttagctgtg tcgagtttct
     1921 tcatcaagct gcgtgtacga agtacagatg ttgctgacat agagaactca tccaagccca
     1981 ttccgacaag aagtggaaca gcttgttggt caccagccat ctcaccacac ataccagccc
     2041 atttaccttc agcgtgagct gctttgatca cattgttaat caagcgtagg attgatgggt
     2101 tgtatggttg gtaaaggtat gaaacttgtt cgttcatacg gtctgctgcc attgtatatt
     2161 ggatcaagtc gtttgtacca attgagaaga agtcaacttc tttagcaaat tggtctgcaa
     2221 gcatagctgc tgcaggaatc tcgatcatga taccaacttg gatgttatcc gcaactgcaa
     2281 caccttcagc aagaaggttt gctttttctt catcaaagac tgctttcgct gcacggaatt
     2341 ctttcaagag cgcaaccatt gggaacatga tacgcaattg accgtgaaca gacgcacgaa
     2401 gaagagcacg gatttgtgtg cggaacatag catctccagt ctcagagata gagatacgaa
     2461 gagcacggaa tccaaggaat gggttcattt cgtgaggcat atcgaagtaa ggaagttcct
     2521 tatctccacc gatatccatt gtacgaacga caacaggttt accgttcatt ccttcaagaa
     2581 cagccttgta tgcttcatac tgctcatctt cagttgggaa gtcttgagaa tccatgtaca
     2641 agaactctgt acggtaaagt ccaacagctt ctgcaccgtt gttgttaaca ccttcaacgt
     2701 cttttggagt accaatattg gcagccaact cgaagtgttt accgtcagca gtcactgttt
     2761 gagcatcttt caaaagtgcc cattcagctt tttgtttcgc ataggcttca ccagctgctt
     2821 taaattctgc cgcttgttca tctgttgggt tgataatcac ttctccagtg atcccgttaa
     2881 cagcaaggat gtcaccgtct ttaacgattt cagtgatgtt gtttgtacct aatacagcag
     2941 caatttcaag tgtacgtgcc atgatagctg agtggcttgt acgtccacca atgttggtta
     3001 caaaagcttt tacaaagttt ttgtccaatt gagctgtatc tgaaggagtc aagtcatgcg
     3061 caatcacaat cacttcttca ttgatagaag ctgggtttgg caatttttta ccaagaaggt
     3121 ttgccaatac acgttttgtc acgtcgcgga tatccgctgc gcgttcttgc atgtatgggt
     3181 tgtcttccat gccttcaaag atagtgataa acatatctgt aacttctttc agacctgctt
     3241 ctgcattcac tttcttcgca cggatagttt ccttgatttg gctgatcatt tctgggtcag
     3301 caagaaccat taagtgagca tcaaaaactt gagctgcttc ttcaccgagc gtacctactg
     3361 ctttctcgcg aataacagaa agctcgtctt gtgatgcctg tagagcggca tcaaggcgag
     3421 cttcttctgc gtttgtatct tcgactgtaa tagtctcaaa tgacaaatcc ggctgaacga
     3481 gtagatatgc ttttgcaact gcaacaccgt cagatgctgc gattccttta agcatttctg
     3541 tcatttccct tatgccaatc cttctttttc cattgtttct gagattgcag cgatagcgtc
     3601 atctgcatct gcaccttcag ctgagatagt tacgtcagca ccttggccaa caccaagact
     3661 cataacaccc ataattgatt taaggttaac tgatttacct ttgtactcaa gagtgatatc
     3721 tgaagcaaat ttgctagcag tttgtaccaa caatgttgct ggacgtgcgt gaatacctgt
     3781 ttctgccact acgtggaaat ctttagaagc catagtttga ctctcctttt gttctttctt
     3841 tttttgagtt atatgtgata acccttacaa tttagtatta taccatcttc taggattttt
     3901 ttcaagcatt ttctggattt tccttcaatt tcctttcaga taacttgctg aaaatcttct
     3961 atcctagata acaaaacaca ggatatagtt tcttcaaaaa caatttatag gataatcatt
     4021 gct
//
LOCUS       DAVLXF010000122         3847 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLXF010000122 DAVLXF010000000
VERSION     DAVLXF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.122"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..429
                     /locus_tag="VYO12_001973"
     CDS             <1..429
                     /locus_tag="VYO12_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1588505.1"
                     /translation="DHVRKNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSA
                     DNLYKPSTDTEETEEEAEDTTDETEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAM
                     ETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            639..3758
                     /gene="phtE"
                     /locus_tag="VYO12_001974"
     CDS             639..3758
                     /gene="phtE"
                     /locus_tag="VYO12_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1588506.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNLADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1438 a          627 c          756 g         1026 t
ORIGIN      
        1 gaccatgttc gtaaaaataa ggcagaccaa gacagtaaac ctgatgaaga taagggacat
       61 gatgaagtaa gtgagccaac tcaccctgaa tctgatgaaa aagagaatca cgctggttta
      121 aatccttcag cagataatct ttataaacca agcactgata cggaagagac agaggaagaa
      181 gctgaagata ctacagatga gactgaaatt cctcaagtag agcattctgt gattaacgct
      241 aagatagcag aggcggaggc cttgctagaa aaagtaacag attctagtat tagacaaaat
      301 gctatggaga cattgactgg tctaaaaagt agtcttcttc tcggaacgaa agataataac
      361 actatttcag cagaagtaga tagtctcttg gctttgttaa aaaaaagtca accggctccg
      421 atacagtagt aaaatgaatg gagcatattt tatggagaag taacctttcg tgttacttct
      481 cttttttaga aaaacgtaac agaaacttga caaaagtaat tttaaatagt aaactattta
      541 ctggttaatt aaatggttaa ataaccggtt tagaaaacta tttaataaag taaaagaagt
      601 tgagaaaaaa cttcatcatt tattgaaatg agggatttat gaaatttagt aaaaaatata
      661 tagcagctgg atcagctgtt atcgtatcct tgagtctatg tgcctatgca ctaaaccagc
      721 atcgttcgca ggaaaataag gacaataatc gtgtctctta tgtggatggc agccagtcaa
      781 gtcagaaaag tgaaaacttg acaccagacc aggttagcca gaaagaagga attcaggctg
      841 agcaaattgt aatcaaaatt acagatcagg gctatgtaac gtcacacggt gaccactatc
      901 attactataa tgggaaagtt ccttatgatg ccctctttag tgaagaactc ttgatgaagg
      961 atccaaacta tcaacttaaa gacgctgata ttgtcaatga agtcaagggt ggttatatca
     1021 tcaaggtcga tggaaaatat tatgtctacc tgaaagatgc agctcatgct gataatgttc
     1081 gaactaaaga tgaaatcaat cgtcaaaaac aagaacatgt caaagataat gagaaggtta
     1141 actctaatgt tgctgtagca aggtctcagg gacgatatac gacaaatgat ggttatgtct
     1201 ttaatctagc tgatattatc gaagatacgg gtaatgctta tatcgttcct catggaggtc
     1261 actatcacta cattcccaaa agcgatttat ctgctagtga attagcagca gctaaagcac
     1321 atctggctgg aaaaaatatg caaccgagtc agttaagcta ttcttcaaca gctagtgaca
     1381 ataacacgca atctgtagca aaaggatcaa ctagcaagcc agcaaataaa tctgaaaatc
     1441 tccagagtct tttgaaggaa ctctatgatt cacctagcgc ccaacgttac agtgaatcag
     1501 atggcctggt ctttgaccct gctaagatta tcagtcgtac accaaatgga gttgcgattc
     1561 cgcatggcga ccattaccac tttattcctt acagcaagct ttctgcctta gaagaaaaga
     1621 ttgccagaat ggtgcctatc agtggaactg gttctacagt ttctacaaat gcaaaaccta
     1681 atgaagtagt gtctagtcta ggcagtcttt caagcaatcc ttcttcttta acgacaagta
     1741 aggagctctc ttcagcatct gatggttata tttttaatcc aaaagatatc gttgaagaaa
     1801 cggctacagc ttatattgta agacatggtg atcatttcca ttacattcca aaatcaaatc
     1861 aaattgggca accgactctt ccaaacaata gtctagcaac accttctcca tctcttccaa
     1921 tcaatccagg aacttcacat gagaaacatg aagaagatgg atacggattt gatgctaatc
     1981 gtattatcgc tgaagatgaa tcaggttttg tcatgagtca cggagaccac aatcattatt
     2041 tcttcaagaa ggacttgaca gaagagcaaa ttaaggctgc gcaaaaacat ttagaggaag
     2101 ttaaaactag tcataatgga ttagattctt tgtcatctca tgaacaggat tatccaagta
     2161 atgccaaaga aatgaaagat ttagataaaa aaatcgaaga aaaaattgct ggcattatga
     2221 aacaatatgg tgtcaaacgt gaaagtattg tcgtgaataa agaaaaaaat gcgattattt
     2281 atccgcatgg agatcaccat catgcagatc cgattgatga acataaaccg gttggaattg
     2341 gtcattctca cagtaactat gaactgttta aacccgaaga aggagttgct aaaaaagaag
     2401 ggaataaagt ttatactgga gaagaattaa cgaatgttgt taatttgtta aaaaatagta
     2461 cgtttaataa tcaaaacttt actctagcca atggtcaaaa acgcgtttct tttagttttc
     2521 cgcctgaatt ggagaaaaaa ttaggtatca atatgctagt aaaattaata acaccagatg
     2581 gaaaagtatt ggagaaagta tctggtaaag tatttggaga aggagtaggg aatattgcaa
     2641 actttgaatt agatcaacct tatttaccag gacaaacatt taagtatact atcgcttcaa
     2701 aagattatcc agaagtaagt tatgatggta catttacagt tccaacctct ttagcttaca
     2761 aaatggccag tcaaacgatt ttctatcctt tccatgcagg ggatacttat ttaagagtga
     2821 accctcaatt tgcagtgcct aaaggaactg atgctttagt cagagtgttt gatgaatttc
     2881 atggaaatgc ttatttagaa aataactata aagttggtga aattaaatta ccgattccga
     2941 aattaaacca aggaacaacc agaacggccg gaaataaaat tcctgtaacc ttcatggcaa
     3001 atgcttattt ggacaatcaa tcgacttata ttgtggaagt acctatcttg gaaaaagaaa
     3061 atcaaactga taaaccaagt attctaccac aatttaaaag gaataaagca caagaaaact
     3121 caaaacttga tgaaaaggta gaagaaccaa agactagtga gaaggtagaa aaagaaaaac
     3181 tttctgaaac tgggaatagt actagtaatt caacgttaga agaagttcct acagtggatc
     3241 ctgtacaaga aaaagtagca aaatttgctg aaagttatgg gatgaagcta gaaaatgtct
     3301 tgtttaatat ggacggaaca attgaattat atttaccatc gggagaagtc attaaaaaga
     3361 atatggcaga ttttacagga gaagcacctc aaggaaatgg tgaaaataaa ccatctgaaa
     3421 atggaaaagt atctactgga acagttgaga accaaccaac agaaaataaa ccagcagatt
     3481 ctttaccaga ggcaccaaac gaaaaacctg taaaaccaga aaactcaacg gataatggaa
     3541 tgttgaatcc agaagggaat gtggggagtg accctatgtt agatccagca ttagaggaag
     3601 ctccagcagt agatcctgta caagaaaaat tagaaaaatt tacagctagt tacggattag
     3661 gtttagatag tgttatattc aatatggatg gaacgattga attaagattg ccaagtggag
     3721 aagtgataaa aaagaattta tctgatctca tagcgtaagg aatagcagta gaaaaagtct
     3781 gaatcaaaaa tgaagttctc tcaaaagtta gaaataaaac tctgactttg ggagaatttc
     3841 attttat
//
LOCUS       DAVLXF010000123         3676 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLXF010000123 DAVLXF010000000
VERSION     DAVLXF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.123"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            113..340
                     /locus_tag="VYO12_001975"
     CDS             113..340
                     /locus_tag="VYO12_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588507.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            628..3525
                     /gene="nanA"
                     /locus_tag="VYO12_001976"
     CDS             628..3525
                     /gene="nanA"
                     /locus_tag="VYO12_001976"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1588508.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTPE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLDGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPKRENGMVHLARVEENGELTWIKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1252 a          621 c          834 g          969 t
ORIGIN      
        1 tgatagaatc tatctttatg tatatttaaa gatagattgc tgtaaaaata gtagtagcta
       61 tgcgaaataa cagatagaga gaagggattg aagcttagaa aaggggaata atatgatatt
      121 taaggcattc aagacaaaaa agcagagaaa aagacaagtt gaactacttt tgacagtttt
      181 tttcgacagt tttctgattg atttatttct tcacttattt gggattgtcc cctttaagct
      241 ggataagatt ctgattgtga gcttgattat atttcccatt atttctacaa gtatttatgc
      301 ttatgaaaag ctatttgaaa aagtgttcga taaggattga gcaggaagta tggtataaat
      361 agcataagct gatgtccatc atttgcttat aaagagatat tttagtttaa ttgcagcggt
      421 gtcctggtag ataaactaga ttggcaggag tctgattgga gaaaggagag gggaaaattg
      481 gcaccaattt gagatagttt gtttagttca tttttgtcat ttaaatgaac tgtagtaaaa
      541 gaaagttaat aaaagacaaa ctaagtgcat tttctggagt aaatgtctta tttcagaaat
      601 cgggatatag atatagagag gatcagtatg aatcggagtg ttcaagaacg taagtgtcgt
      661 tatagcatta ggaaactatc ggtaggagcg gtttctatga ttgtaggagc agtggtattt
      721 ggaacgtctc ctgttttagc tcaagaaggg gcaagtgagc aacctctggc aaatgaaact
      781 caactttcgg gggagagctc aaccctaact gatacagaaa agagccagcc ttcttcagag
      841 actgaacttt ctggcaataa gcaagaacaa gaaaggaaag ataagcaaga agaaaaaatt
      901 ccaagagatt actatgcacg agatttggaa aatgtcgaaa cagtgataga aaaagaagat
      961 gttgaaacca atgcttcaaa tggtcagaga gttgatttat caagtgaact agataaacta
     1021 aagaaacttg aaaacgcaac agttcacatg gagtttaagc cagatgccaa ggctccagca
     1081 ttctataatc tcttttctgt gtcaagtgct actaaaaaag atgagtactt cactatggca
     1141 gtttacaata atactgctac tctagagggg cgtggttcgg atgggaaaca gttttacaat
     1201 aattacaacg atgcaccctt aaaagttaaa ccaggtcagt ggaattctgt gactttcaca
     1261 gttgaaaaac cgacaccaga actacctaaa ggccgagtgc gcctctacgt aaacggggta
     1321 ttatctcgaa caagtctgaa atctggcaat ttcattaaag atatgccaga tgtaacgcat
     1381 gtacaaatcg gagcaaccaa gcgtgccaac aatacggttt gggggtcaaa tctacagatt
     1441 cggaatctca ctgtgtataa tcgtgcttta acaccagaag aggtacaaaa acgtagtcaa
     1501 ctttttaaac gctcagattt agaaaaaaaa ctacctgaag gagcggcttt aacagagaaa
     1561 acggacatat tcgaaagcgg gcgtaacggt aaaccaaata aagatggaat caagagttat
     1621 cgtattccag cacttctcaa gacagataaa ggaactttga tcgcaggtgc agatgaacgc
     1681 cgtctccatt cgagtgactg gggtgatatc ggtatggtca tcagacgtag tgaagataat
     1741 ggtaaaactt ggggtgaccg agtaaccatt accaacttac gtgacaatcc aaaagcttct
     1801 gacccatcga tcggttcacc agtgaatatc gatatggtgt tggttcaaga tcctgaaacc
     1861 aaacgaatct tttctatcta tgacatgttc ccagaaggga agggaatctt tggaatgtct
     1921 tcacaaaaag aagaagccta caaaaaaatc gatggaaaaa cctatcaaat cctctatcgt
     1981 gaaggagaaa agggagctta taccattcga gaaaatggta ctgtctatac accagatggt
     2041 aaggcgacag actatcgcgt tgttgtagat cctgttaaac cagcctatag cgacaagggt
     2101 gatctataca agggtgacca attactaggc aatatctact tcacaacaaa caaaacttct
     2161 ccatttagaa ttgccaagga tagctatcta tggatgtcct acagtgatga cgacgggaag
     2221 acatggtcag cgcctcaaga tattactccg atggtcaaag ccgattggat gaaattcttg
     2281 ggtgtaggtc ctggaacagg aattgtactt cggaatgggc ctcacaaggg acggattttg
     2341 ataccggttt atacgactaa taatgtatct cacttagatg gctcgcaatc ttctcgtgtc
     2401 atctattcag atgatcatgg aaaaacttgg catgctggag aagcggtcaa cgataaccgt
     2461 caggtagacg gtcaaaagat ccactcttct acgatgaaca atagacgtgc gcaaaataca
     2521 gaatcaacgg tggtacaact aaacaatgga gatgttaaac tctttatgcg tggtttgact
     2581 ggagatcttc aggttgctac aagtaaagac ggaggagtga cttgggagaa ggatatcaaa
     2641 cgttatccac aggttaaaga tgtctatgtt caaatgtctg ctatccatac gatgcataat
     2701 ggaaaagaat acattatcct cagcaatgca ggtggaccga aacgtgaaaa tgggatggtc
     2761 cacttggctc gtgtcgaaga aaatggtgag ttgacttgga tcaaacacaa tccaattcaa
     2821 aaaggagagt ttgcctataa ttcgctccaa gaattaggaa atggggagta tggcatcttg
     2881 tatgaacata ctgaaaaagg acaaaatgcc tataccctat catttagaaa atttaattgg
     2941 gactttttga gcaaagatct gatttctcct accgaagcga aagtgaagcg aactagagag
     3001 atgggcaaag gagagatggg caaaggagtt attggcttgg agttcgactc agaagtattg
     3061 gtcaacaagg ctccaaccct tcaattggca aatggtaaaa cagcgacttt cctaacccag
     3121 tatgatagca agaccttgtt atttgcagta gataaggaag atatcggaca ggaaattatt
     3181 ggtatagcta aaggaagcat cgaaagtatg cataatcttc ctgtaaatct agcaggtgcc
     3241 agagttcctg gcggagtaaa tggtagcaaa gcagcggtgc atgaagttcc agaatttaca
     3301 gggggagtta atggtacaga gccagctgtt catgaaatcg cagagtataa gggatctgat
     3361 tcgcttgtaa ctcttactac aaaagaagat tatacttaca aagctcctct tgctcagcag
     3421 gcacttcctg aaacaggaaa caaggagagt gacctcctag cttcactagg actaacagct
     3481 ttcttccttg gtctgtttac gctagggaaa aagagagaac aataagagaa gaattctaaa
     3541 catttgattt tgtaaaaata gaaggagata gcaggttttc aagcctgcta tctttttttg
     3601 atgacattca ggctgatacg aaatcataag aggtctgaaa ctactttcag agtagtctgt
     3661 tctataaaat atagta
//
LOCUS       DAVLXF010000124         3405 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLXF010000124 DAVLXF010000000
VERSION     DAVLXF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.124"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(189..800)
                     /gene="rpsD"
                     /locus_tag="VYO12_001977"
     CDS             complement(189..800)
                     /gene="rpsD"
                     /locus_tag="VYO12_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1588509.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(995..2047)
                     /locus_tag="VYO12_001978"
     CDS             complement(995..2047)
                     /locus_tag="VYO12_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1588510.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2044..2742)
                     /locus_tag="VYO12_001979"
     CDS             complement(2044..2742)
                     /locus_tag="VYO12_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1588511.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2909..>3405)
                     /locus_tag="VYO12_001980"
     CDS             complement(2909..>3405)
                     /locus_tag="VYO12_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV1588512.1"
                     /translation="ALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDN
                     IVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTN
                     TMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNK
                     KEEN"
BASE COUNT          942 a          765 c          651 g         1047 t
ORIGIN      
        1 ccataaaaga ttataccaca ttgtgtacta tttttggttc attttactat atcactttgg
       61 actatatcgg tctagttatg tctaaacgat tatagctgga tactaagaga aatcaaaaaa
      121 gcactaaggg agaacgtccc aaagtgctta gtttcaaggc tttacagcct atcttattta
      181 ataaaatatt acaacatttt gttgtagaat tcaacgacaa gtgcttcgtt gatttctggg
      241 ttgatttcgt cgcgttctgg caagcgagtc aatgaacctt ccaatttttc agcgtcgaat
      301 gatacgaatg ctggacgtcc aagagtagct tctactgctt caaggattgc tggaactttc
      361 aatgattttt cacgaactga gatcacttga cctggagtta cgcggtatga tgggatatca
      421 acgcgtttcc cgtcaacaag gatgtgaccg tggtttacaa attgacgagc ttgacgacga
      481 gtagtcgcga gaccaagacg gtaaacaacg ttatccaaac gacgttccaa aagaagcata
      541 aagttgaaac ctaggattcc gcctttgatt tttgtagctt gtacaaacaa gttacggaat
      601 tgtttttcac ctacaccgta agtgaaacga agtttttgtt tttcagccaa ttgcaaaccg
      661 tattctgaca atttagaacg gttgtttggt ccgtgttgtc ctggtacgta gttacgacgt
      721 gccaattctt tacctgtacc tgtaagtgaa aggccaaggc gacgagcttg tttccaagat
      781 ggtcctgtat aacgtgacat atgaatgtcc tcctgatata aataatattt cggtggaaat
      841 agtcacttag aaagccctga ttcgtgcaga tgcccttcgc ctaaacagcc aaggttactt
      901 atcataagac acctgttgac gagcttcatg ctttcctgct gatatttcac acaaaattca
      961 ttctaccatg aatagctgga tttgtaaagg ggttttaggc tttattttca ctaccagaga
     1021 ggttgagaac gagagtaaag gtacttccta gaccgtactg gctgctgact gtgatttccc
     1081 cacccaattg atgggccaat tcacgcgcaa tcgcaagtcc taatccatga ccacctgtct
     1141 tcatgttacg cgaagtttcg acacgataaa ggcgtttgaa aatattttcc aaatcctctg
     1201 gggcaatgcc ctgcccttca tcggtcacac tgattgaaag ctggcccttc tccagcttag
     1261 ccaccacttc cagcttggtt cctggagcag aatatttaaa agcgttatcg accagattca
     1321 ccaagatacg agaaagctta gcataatctc cctcaatccg ggcagactct gggattacct
     1381 gcaagtggac atctcttctc tcctgctcaa tcaaaaactg aaattcactc atgcactcaa
     1441 ttaagagctt gtccagaaaa atactgtctt tactggtagt ttccacctga tttctagctg
     1501 tgtttagggt caaaaaattc aactcctcaa ccagtttatt gagcctctcc gtctggcgtc
     1561 caatggttgc tagataatga gcttgctccg actccttgat aatcccatcc aaaatccctt
     1621 ctaccgtcgc ttggatcgaa gtgataggag tcttaatatc atgcgacaac tgagcaatca
     1681 tcaagccctt ttctcgttcg ctttcttcca aggaatcaaa gcttacctgc aaatcatggg
     1741 acatctcatt aaaagtttgc cctaattgct gaaattctac aggaccttga acctccaaat
     1801 ttgaaggaaa atccttggcc gctacccgct tggcatgctc cttgagtttg cccaacgacg
     1861 taaagactgg caataggaga aagagactaa tcccagcacc gacaaggctg gcaacgatgg
     1921 tcatcccaag caaaaagtaa atctcgcctt tcgcaatcag cattttttga acagcccaaa
     1981 aaacaaccaa aatggttaag agggttgaaa taatatatcc aaccaaaata taacttttta
     2041 gtttcatgtt tgtcctctcg gtttctctat cttatatccc aacccccaaa ctgtcttaat
     2101 agtgggcgtt tggtcactac tatattttgc cagctcctgt cgaagagcat ggatatgcac
     2161 attcaaggta ttggtgtcat ccacgtagtc ttctttccag atcttttcat agaggtctgt
     2221 cttggagaaa actcgctctg gattgctagc taaaatccac agcaattcaa atgatttaac
     2281 agttaaatcc agcatttctt ctcctatttg aacttcatga ctactatgat tcatttttag
     2341 attgccaagg gaaatcagct ctgtttcgcc cccacgatga aggcggcgca aaatattgtg
     2401 gacacgcaaa actagctcac gtgggctaaa aggcttagca ataaaatcat ctgctcccaa
     2461 gctcaggccg taaatcttgt cctgctcact ggtcttagca gtaataaata ggaaaggctg
     2521 ctctggtgat aagtattgaa cctcactgat taaatcataa ccatccatcc gaggcatcat
     2581 gacatctgtg ataatcaaat caatcggttt tttcttgaac agctctagcg cttccagtcc
     2641 atcatgggct accaagacct gataacctgc ctgaattaag tatctctgat gaatatctgt
     2701 gatttctacc tcgtcgtcaa cgagtaaaat tgtctttccc atctgtctct cctttgataa
     2761 aaacagtgct atactgcttt aagtataaca ctatttttat tgttttacga tgattttaat
     2821 ctgctattga aagaaagtcc tgattgctga ttgagttgtt aaactaatct tagtcagccc
     2881 actatccatc atttatttcc ttagctgatt agttttcttc ttttttgttt ttcaaaccta
     2941 gaccaccgag taaacctgca agcgcaagcc caaggaaacc aatacttgcc attgatgttt
     3001 gagtctcacc agtatttggt agcatagctt tatcctctga catcatcgta tcagacatct
     3061 tgttagcaga agcagccata ttttcacctg ccatcgtgtt ggtagaactt gtcatggtgt
     3121 cagcaggcat gctatctgta gtacctgtag catgattgtg attcatcgga gtcacgccag
     3181 aaccagagtt agaaggtgat aatgaaccat ttgctgtgtc tgatgtttct ttaacattta
     3241 tcttaatagt gactttttta gttgctacga tgttgtccaa gtctggttta ccgtctttgt
     3301 taccatagac attgactgta gcactgtaag tttgagtacc atttgctcgg aactggtcaa
     3361 tgagcgcttg tttttctttg ccagctacat ttccgtccaa ggcta
//
LOCUS       DAVLXF010000125         3401 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLXF010000125 DAVLXF010000000
VERSION     DAVLXF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.125"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(381..1577)
                     /gene="tuf"
                     /locus_tag="VYO12_001981"
     CDS             complement(381..1577)
                     /gene="tuf"
                     /locus_tag="VYO12_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1588513.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2499..3368)
                     /locus_tag="VYO12_001982"
     CDS             complement(2499..3368)
                     /locus_tag="VYO12_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1588514.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
BASE COUNT         1033 a          684 c          668 g         1016 t
ORIGIN      
        1 actattgtat aatgcttttt gatttgagtc tagtattata ctcttcgaaa atctcttcaa
       61 accgcgtcag cttcgccttg tcgtatagat gttactgact tcgtcagttc tatctacaac
      121 ctaaaaacag tgttttgagc agtctgcagc taactttcta taacaaaatg aaataagaac
      181 aagacaaatc aatcaagaca atcaaatcga tttctaacaa tgttttagaa gtagatgtgt
      241 actattctag tttcaatata ttatagtttg ctctttgatt ttcattgagt attagttcaa
      301 tatctaaaaa aagaaccttg ccaagcaaga ttcttttagt gtctgactta aataattgtt
      361 cttctgggaa ctaaatcgaa ttaagcttcg atttctgtaa ccatacctga accaacagta
      421 cgtccaccct cacggataga gaatgtagta ccttgttcta cggcgattgg gtgaatcaac
      481 tcaacgtcga ttgtcacgtt atcaccaggc attaccattt cagtacctgc tggaagttcg
      541 attgaacctg taacgtcagt agtacggaag tagaattgtg gacggtagtt gttgaagaat
      601 ggagtgtgac gtccaccttc ttctttagta aggatgtaga cttcaccttt gaatttagtg
      661 tgtgggttga ttgaacctgg tttagcgata acttgtccac gttcgatttc atcacgttga
      721 acaccacgaa gaaggacacc tacgttatct ccagcaagac cttcgtcaag ttgtttacgg
      781 aacatttcaa caccagtaac aactgctttt tgagtttctt ctttgatacc aacgatttcg
      841 atttcgtcgt tgactttaac gataccacgg tcgatacgtc ctgaagcaac tgtaccacgt
      901 ccagtgattg agaatacgtc ctcgactgga agaagcaatg gtttgtcagt gtcacgttct
      961 ggttctggga tatactcatc aactgtgttc atcaattcca taacgatgtc ttcgtattta
     1021 gagtcacctt caagagcttt aagtgctgaa ccttggataa ctggaagatc gtcacctggg
     1081 aagtcgtatt ctgacaatag gtcacggatt tccatttcaa ccaattcaag caattcttcg
     1141 tcgtcaacca agtcaacttt gttcatgaag acgataaggt gtttaacacc aacctgacgt
     1201 gaaagaagga tgtgctcacg agtttgtggc attggtccgt cagttgaagc tactacaagg
     1261 atagctccgt ccatttgagc agcaccagtg atcatgtttt taacgtagtc cgcgtgtcct
     1321 ggagcgtcga tgtgagcgta gtgacgtttt tcagtttcgt actcaacgtg cgcagtgttg
     1381 atagtgatac cgcgttcgcg ttcttctgga gcagcatcga tagacgcata gtctttaggt
     1441 tggttaactg atgaaggcaa gcgacgtgcc aaaacagttg tgatagctgc agttagggta
     1501 gttttaccgt ggtcaacgtg tccgatagta ccaatgttaa cgtgtggttt actacgatcg
     1561 tatttttctt ttgccatttg agtaaaagcc tccaataaaa tatattttat agatagacag
     1621 taggcaatac agtctaactt tccttactat tttatcaaat ttcagctaaa ttgcaagtgt
     1681 tttacaacgt ttttgtgaat tattcttggt aaaaatttaa ctaattaata gaaagtacta
     1741 aatagactta tccttcaagg agttttcagc attatccatc cactcacttg ctgaaaatgt
     1801 aaacagtctc tggtttatat agtaaaatga aacaagaata gggcaaatcg atcaggacag
     1861 tcaaatcgat ttctaacaat gttttagaag tagatgtgta ctattctagt ttcaatctac
     1921 tatacaacct caaaactgtg ttttgaacaa cctgcggcta gcttcttagt ttgccctttg
     1981 attttcattg agtataaaat tgcagataaa acgataacaa tcataggact cagcagagtc
     2041 aatgtaggtc gaagaatagc ccccctaatc gcttatttta tagcaaaatg aaatagaaac
     2101 tgaacatttt gttgaggaag gtaaatcaat tcctcacaat gtttgagaaa gaagggggga
     2161 ctattccaac ttcaacccac tataaagcag acttacaaga tagggaaccc atctccgata
     2221 atattccgat aatatatagt agaatgaaaa ctgcacaatt tgattggaga attcaaacca
     2281 atttctaaca atgttttaga aacaagggtc tattatttca actgcaacct actatagccc
     2341 attgaagctg gaatagccca ctgagacttc taaaccactg ctggagagga gtttgtttag
     2401 agtttctttc atttccctaa aaaatcaaaa agagagaacc agtctgatcc tctcttactt
     2461 atagatggtg ctcactcaaa tgttaaagga gctatttctt agagataaag gaatttgaat
     2521 acagctactg ccgcaattgc tgctgcgata ggtgctacta ctggtaccca agaataccac
     2581 cattttgaat cgcctttatg ctcaccaaga actgattttg gaaggaaagc atgaaggaga
     2641 cgtggtccca agtcacgggc tgggttcaag gcaggtcctg taggtcctcc aagtgatgtt
     2701 accaaagcca taacgaggaa tccaagtgcc aagtgagcca cagaaagtcc tgaagcagtg
     2761 tgtggagcca cctgtgcttt aatagccaag tcagaaaaat caactgtttg tcctgcttct
     2821 attgcctttt gtttcatgaa ttgaagcact tcagcaccaa agaagttttt agtcaaacca
     2881 agagctgcaa agaacaaaac aaatgaacca acaaactcat taatcaaacc attgacagtt
     2941 gctgcatagc gactttcttt tgtaccatgg tcaatacttg aaatagttga gaaagttccc
     3001 aagatgttat ttgggttttc agttttcaag tagaatggac ggtatgttgc cacaactaag
     3061 gcttggccaa agatagcccc caagacttgc gcgataatgt aaggtaccac ttgtgcccaa
     3121 gggaaaagac cgctaactgc aagccctaga gtgaaagcag ggttgatgtg attcccagat
     3181 acgttaccaa acatcaaggc tgggatcata acccccatac cataaccaac agcgatgacg
     3241 atccagccac tttggtgacc tttcgtacct ttaagttcaa cgttggcaac tgcaccattc
     3301 ccaagaatga tcaaaatggc agttcccaaa aattcagtgg catacttcag tgcccatgtg
     3361 aaatccattt gctagattct ccttaaaatt ttttagatat t
//
LOCUS       DAVLXF010000126         3312 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLXF010000126 DAVLXF010000000
VERSION     DAVLXF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.126"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>3312
                     /locus_tag="VYO12_001983"
     CDS             <1..>3312
                     /locus_tag="VYO12_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1588515.1"
                     /translation="RIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQA
                     QAEAEIAKQVEALSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTA
                     EELASRIASVQASGRNLFLNSLFKQDISKTGIWTTSTYTATIDSESKYLGYNALKIIG
                     LNPSGRDGGNPKVTYPVLGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGY
                     KTGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVDTS
                     YSEAPEDIEGQISTVESTFKQRVNSLEAGVNRLTEGLRTKADISALNVTAENIRQSVK
                     SLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKMK
                     VGGRNLWIKSKTVGAVIEKLPENHVTGQKECYRLENNSTLTFNLEPDFSSRLYQKVTF
                     SAWIKYENVVQGRNFWNVFNCFKHYLFRKNSETGVQSGPDYATLGMYKGSADWKYITF
                     TYDYSEKTNFDQLKTSLRFNLEGATSGTAWVTGIKVEIGSVATDWSPAPEDADGLITE
                     AKATFERTAQGLRTDLSAIQEYVNKNGQRQEALQRYTREESTRQATAVRELVNRDFVG
                     KVTYQEDVKGINQRIEAVKTSANKDIASQIASYRQSVDGKFTDISSQITTYKQDVGGQ
                     ISGLSNRLTSSEQGATTQISNISNRINSNKQGTDNQISNLKTQVATNKDNAERQMGRI
                     SDQVSANKANADSQFANVTNQLVRKVETTDFQRVKETSKLYERILGNTENGIADKVAR
                     MALTNQLFQVEVAKNASNGQNLLKGTKDFSGGWKNKGANWKKHAEKYKGVDVLFKNNS
                     WNGVGQEIDAKIGEVYTFSLWMKSDWKNDTVNFYVNRNGSVEKGWGVPSETSVAITSE
                     WKRYSFTFKITVDGFIFPRVERLNQNTNLYIAGLKLEKGSYATPYTEAPEDTDEAIRS
                     VQSQLTGSWAVQNINSAGDIISGINLGANGHNRFVGKLTHITGETLIDRAVIKSAMVD
                     KLKTANFEAGSVTTTILDAEAVTAEKLKVDDALIRKLTAKDAFIDQLISKRIFSIKVE
                     SVISSSTFLEAYQGR"
BASE COUNT         1095 a          611 c          776 g          830 t
ORIGIN      
        1 gcggattgag ctggattctg ttgctagact tgaagcgttt aagtcgcaga ctacgagcgc
       61 acaaacggct ctgtcaggtg acttggatgt tctaaaacga actatcgcaa acgatattcg
      121 accgaagcaa gcacaggctg aagctgagat tgccaagcaa gttgaagcac ttagccggac
      181 taaaaatgaa ctggctggcg tgaagtcagc gcaagcgacg tatgaggaga cgacgactcg
      241 tagactgtca gaactgacca acttggccaa tggtaaagcc agcaagtcag aactcacgca
      301 gacagctgag gagctagcta gtcggattgc gagtgtgcag gcatccggtc gaaatctatt
      361 cttgaactca ctattcaagc aggatatttc aaaaacagga atttggacaa cgagtacata
      421 tacggctact atcgatagcg aaagtaagta tcttggatat aacgctctta aaattatagg
      481 tctgaatcca tctggccgtg atggagggaa tcccaaggtt acttatccag ttctgggtca
      541 attcgggaaa gtaattcccg gaagtacgac taatcaagat gtaaccatta gtttttatgc
      601 taaggcaaat aaaaatggaa taatgctaag atctcgatta gggaatatcg gatataaaac
      661 tggaaatgtg acattgtcga cagaaattaa acgatatgtt gtccatattc caaaaggttg
      721 gacaaacgaa tccaagcaga ccacaaatga atggttgttc aatttcaacc aggaaggaac
      781 cgtttggatt tggatgccga agtttgaaat aagcgatgta gatacttctt attcagaagc
      841 tcctgaagat atagaaggtc agatttcaac agttgaatcg acctttaaac aacgagtcaa
      901 ctcgctcgaa gctggtgtga atcgtctgac tgaaggcctt agaaccaaag ccgatatcag
      961 cgcactcaac gtgactgctg aaaatatccg gcaatctgtg aagagtcttg agacagacac
     1021 gcagaacaag ctaaatcaga agttgagtca ggctgaattt gaggtgcgag ccggctctat
     1081 ccgtcaggaa atcctgaacg caaccaagga taaggcagat aagactttag ttgtatctga
     1141 agctgggaaa ttgcgtgaag aattttcaaa aatgaaggtg ggaggccgga atctatggat
     1201 aaaatccaag acggttggag ctgtaattga aaaattacct gaaaaccatg tcacaggtca
     1261 aaaagaatgt tataggttag agaacaactc tactttaacg ttcaaccttg aaccagattt
     1321 cagctcaagg ttgtatcaaa aagttacttt tagcgcttgg atcaagtacg aaaatgtagt
     1381 ccaaggtcga aacttttgga atgtatttaa ttgcttcaaa cattatcttt ttagaaaaaa
     1441 tagtgagacc ggagtacaga gtggtccaga ttatgctacg cttggtatgt ataaaggttc
     1501 ggcagattgg aaatatatta cattcactta tgactactct gaaaaaacaa attttgatca
     1561 attgaagaca tcattgcgat tcaatcttga aggtgctaca agcggtacag cttgggtaac
     1621 aggaatcaag gttgaaatcg gtagtgtggc gacggactgg agtcctgcgc ctgaggacgc
     1681 tgatggtctc atcactgaag ctaaggctac ctttgagcgg acagctcagg gcttgcgaac
     1741 cgatttatca gctattcagg aatatgtaaa taaaaacggt cagcgacagg aagccctaca
     1801 gcgctatact cgtgaggaga gcacgagaca agcgacagca gtccgtgagc tggtcaatcg
     1861 tgatttcgtt ggtaaggtta cttatcaaga agatgttaag ggtatcaatc agaggattga
     1921 agctgttaaa actagtgcga ataaagacat cgctagtcaa atcgctagct atcgtcaatc
     1981 tgtagatggt aagttcacgg atatttcaag tcagataact acttataagc aagatgtggg
     2041 cggtcaaatc agtggtctat caaatagact tacaagcagt gagcaaggag ccactactca
     2101 gatttcaaat atttcaaatc ggataaacag taataaacaa ggcacagata atcagatttc
     2161 aaatttaaag actcaggtcg ctacaaacaa ggataatgct gaacgacaaa tgggtagaat
     2221 atctgatcag gtttctgcaa acaaagcgaa tgctgatagt caatttgcga atgtgaccaa
     2281 tcaactagtg cgaaaagtag agactactga cttccagcgt gttaaggaaa ccagtaaact
     2341 ttacgagcgg attttgggca atactgaaaa tggaattgcg gataaggttg ctcgcatggc
     2401 tctgaccaat caactgtttc aggttgaggt ggctaaaaat gccagcaatg gtcaaaattt
     2461 gttgaaaggc acaaaagact tctcaggggg ttggaaaaac aagggtgcaa attggaaaaa
     2521 gcacgctgaa aaatacaaag gtgttgatgt cctatttaaa aataattcgt ggaatggcgt
     2581 tggacaagag attgatgcaa aaattggtga agtctataca ttcagcttat ggatgaagag
     2641 cgactggaag aatgatacag taaatttcta tgtaaataga aatggatctg ttgagaaagg
     2701 ttggggtgtt ccatctgaaa catcggtcgc tataacaagt gaatggaaac gttactcatt
     2761 taccttcaaa attactgtgg acggcttcat ctttcctcgt gtagaacgac ttaatcaaaa
     2821 tacaaatcta tatattgcag gtcttaaact tgaaaaagga tcgtatgcaa caccgtacac
     2881 cgaagctcct gaagacacgg acgaagcgat tcgctctgtt caaagccaac taactggctc
     2941 atgggcagtt caaaacatca actcggctgg agatatcatc tctggaatca atcttggcgc
     3001 caatggacat aaccgctttg ttgggaaatt gacccacatc actggagaga ccctgattga
     3061 cagagcagtc atcaagtctg ccatggttga taagctcaaa acggccaatt ttgaagctgg
     3121 ttcggtcacg actacgatat tagacgctga agcggtaact gctgagaagt tgaaagttga
     3181 cgatgcgctt attagaaaat taactgcaaa agatgctttt attgaccaac tgatatctaa
     3241 acgtatcttc tctattaagg ttgagtccgt catttctagc tcaaccttcc tagaagccta
     3301 tcaaggccga at
//
LOCUS       DAVLXF010000127         3294 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLXF010000127 DAVLXF010000000
VERSION     DAVLXF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.127"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..1280
                     /locus_tag="VYO12_001984"
                     /pseudo
     CDS             1..1280
                     /locus_tag="VYO12_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1420..1713)
                     /gene="rpmA"
                     /locus_tag="VYO12_001985"
     CDS             complement(1420..1713)
                     /gene="rpmA"
                     /locus_tag="VYO12_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1588516.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(1730..2074)
                     /locus_tag="VYO12_001986"
     CDS             complement(1730..2074)
                     /locus_tag="VYO12_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1588517.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(2090..2404)
                     /gene="rplU"
                     /locus_tag="VYO12_001987"
     CDS             complement(2090..2404)
                     /gene="rplU"
                     /locus_tag="VYO12_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1588518.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEADQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(2440..2527)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(2622..3221)
                     /locus_tag="VYO12_001988"
     CDS             complement(2622..3221)
                     /locus_tag="VYO12_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1588519.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
BASE COUNT         1012 a          708 c          555 g         1019 t
ORIGIN      
        1 ttactagaca ttaaagaccc taatatccag attttagaca tcatcaataa ggatacacac
       61 aaggaaatca tcgccaaact ggactacgac gccccatctt gccctgagtg cggaaatcaa
      121 ttgaagaaat atgactttca aaaaccttct aaaattcctt atcttgaaac gactggtatg
      181 cctactagaa ttctccttag aaagcgtcgt ttcaagtgct atcactgttc aagaatgatg
      241 gtcgctgaaa cttctatcgt caagaagaat caccaaattc ctcgtattat caaccaaaaa
      301 attgcgcaaa agttgattga gaagatttct atgaccgaca ttgatcatca gttgtctatt
      361 tcaacttcaa ctgtcattcg caagatcaat gattttcact ttaaacatga tttttcttgt
      421 cttcctgaga ttatgtcttg ggatgagtat gccttcacta agggaaagat gagtttcatt
      481 gcgcaagatt ttgataatct taatattatc actgttcttg aaggcagaac acaagctgtc
      541 atccgaaatc actttcttcg ctacgataga gccgttcgtt gtcaagtgaa aatcattacg
      601 atggatatgt ttagtcctta ctatgacttg gctaaacagc ttcgctttca aatttctagg
      661 ctcaggctga aacagtctcc cagactgttt cactcccgaa tgctaaaatc gttcttgatc
      721 gctttcacat tgtgcaacat cttagccgtg ctatgagtcg tgtgcgtgtt caaatcatga
      781 atcagtttca tcgaaaatcc catgaataca aggctatcaa gcgctactgg aaactcattc
      841 aacaggatag ccgtaaactg agtgataaac gtttttatcg tcctactttt cgcatacact
      901 taacaaataa agaaattctt gacaagattt taagctattc agaagacttg aaacaccact
      961 atcagatcta tcaactctta ctttttcact ttcagaacaa agaccctgag aaatttttcg
     1021 gactcattga ggacaatctg aagcaggttc atcctatttt tcagactgtc tttaaaacct
     1081 ttctaaagaa caaagaaaaa atcgtcaacg ctcttcaatt accttattcc aacgcaaaat
     1141 tggaagcgac caataatctc atcaaactta tcaaacgaaa cgcctttgga tttcggaact
     1201 ttgaaaactt caaaaaaagg atttttatcg ctctgaacat caaaaaagaa aggacgaatt
     1261 ttgtcctttc tcgagcttag ctgacttcaa cccactacag ttgacaaaga gcctaaaaaa
     1321 acaggctctc cgaaaactcg gaaagcctta ttttaagctg tttctagctt cctcaccttt
     1381 atatttcaag gttcgggata aaaggttcaa tggacctttt tatttagcga ttgggtaaac
     1441 agaaacttgt tttttatcgc gtcctttacg ttcaaagcgt actacgcctt caactttagc
     1501 gaacaaagta tcgtctccac cacgaccaac gtttacacct ggatagatgt gtgtaccacg
     1561 ttgacggtaa aggattgatc cacctgttac agtttgtccg tcagctgctt tagctccaag
     1621 acgtttcgct tgtgaatcac gtccgtttga tgtagaacct ccaccttttt tgtgggcgaa
     1681 aagttgcaag ttgttaagag tcatttttaa cataatgttt tcctccgtgt tagttttctg
     1741 tgataactct ggtttggacg aactcagaag agttctccga taagtttgcc atacctaaga
     1801 aaaatgattc aaagaataac tgggtcattt ctctctggtg tgaaggaaga tcttttggta
     1861 tttcaaccat cagatagcca ccttcatctt cgtttaattc taggattggt tcatagcctg
     1921 caaatttctc aatagaattg ataaagttaa tggcaagcgt agaaaccgat gcacacacga
     1981 catctaagcc gtattcgcca ctctcggcgt gtccagtaat ttccgcactc ctcagctcgc
     2041 catcttcggc tctctcaaag actgcctgta tcatgtgttc tccttaaaat taagcgttga
     2101 ttgcgttgat gacaactttt gtatatggtt gacggtgacc ttgtttacgg tggctacctt
     2161 ttttaggttt gtacttgtaa gtaaccactt tcttttgttt tccttgtttt tcaacagttc
     2221 caactacagt agctccagca acaagtggag ttccgacaac agtgttttca ccaccaacaa
     2281 gaacaacttc gttaaaagta acttcttgat cagcttcaac gttcaatttt tcaacgtaaa
     2341 ctgcttgacc aacttcaact ttaacttgtt ttccgccagt tttgataatt gcgtatgtgc
     2401 tcattatgca cctcctatga tttttatggg tttccccgat tttttgtgaa gactcgccta
     2461 gcatcgtggg acgaaccact taaacttgaa taatcaagca acgatgttcg tgcggttgca
     2521 caggatcgtg catagtcaac tcttcaagta tagcatatct cctattttct tacaagtaat
     2581 aacacctaaa atgaagcttt ttcttttact tttttctgcc actaggcaaa aagcatactg
     2641 agataaaaaa tgctcatcat aataggaaca ccaagaatgg tcttttcatg atagaaaatc
     2701 gtcaaatagg ctgaaaagac atcgccaagg acaaaactac taagcaggct aacaaatatg
     2761 aacccttcac gcaaaaaagg agtgtgctta gttcggaaat aatctccaaa agctagcatt
     2821 gttcgtttga tattccctgt cataaaggca ttattgtagg caatacccga aacctcacca
     2881 aaagcagttg tcaccagtcc catacagaag gccaagggct gcactagata gatattatct
     2941 acagtttgtg gcacaaaacc aataattatt gataggattg ctaaaggaat caaggacaga
     3001 ataggttttt tcacaattct caatttttcc ttataaatcg ttaataaaaa gactcccatt
     3061 ataaacgtta ttaaggttaa aagcttatcc ctaacatccg aaacattatt ttgtattagc
     3121 tctaccgaaa gaaagacaac atttccagct tgtccagcta caagggtatt ccctcgaaca
     3181 ataaaagtgt aagcatccac atatccagca caaaatgtca aaaaagtgct aaccttttag
     3241 actgacgtga tatttttctt ataggtaata acctcatttt ccctcccatt gtat
//
LOCUS       DAVLXF010000128         3263 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLXF010000128 DAVLXF010000000
VERSION     DAVLXF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.128"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(474..1007)
                     /locus_tag="VYO12_001989"
     CDS             complement(474..1007)
                     /locus_tag="VYO12_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1588520.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1096..1872)
                     /gene="recX"
                     /locus_tag="VYO12_001990"
     CDS             complement(1096..1872)
                     /gene="recX"
                     /locus_tag="VYO12_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1588521.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQVSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1910..>3263
                     /gene="rlmD"
                     /locus_tag="VYO12_001991"
     CDS             1910..>3263
                     /gene="rlmD"
                     /locus_tag="VYO12_001991"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1588522.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1048 a          561 c          575 g         1079 t
ORIGIN      
        1 aatctaaaga atctggaatt aaacaaatgg acaatgtcat aaaatatttt gagtttattg
       61 aatctaaaag tattgcttta tattttcaaa aacgattaaa tgagctgata gattaaatat
      121 cattttctct gttaagatat tgtttttaaa atattgtact aaatgattga tgctatgtgg
      181 aaatacaaaa aaatgttttt gatacgaagt tgacctgtat tttttatact aatcattttc
      241 gtattttttg tattaaacga tataagtttg ttgtaaactt actaggaata aagacattaa
      301 aaaataatag tatatctatt tgttttatat attttacgaa ttctgcataa atctctttct
      361 agtaatgtgt tgtaactctg ctataatgga tttattcctt tttgtgttta cacaatttat
      421 tttatactac caaaaaaaca aggtcaggat tttgttcctg acctttgaca actttaccga
      481 ttctttagtt ctacatagcg cttgtaccaa atgtttacat aggcttctga gaaaggacca
      541 cgtccattgt taatccaatc aacaagaatt ttgacatgtt cttttaaaat atagtccaag
      601 tcatcagaat aattcatttt gcgtttgtga cgctcgtatt cttcaacgtc caagagacgt
      661 ttttccccat ctgtaaaaat tttaacatcc aaatcgtaat caatatactt cagtgcttct
      721 tcatccagat agtaggggct agccatattg caatagtaag aagttccatt atcacgaatc
      781 atggcaatga tattaaacca atatttcttg tgaaagtaaa caatagccgg ttctcgagtg
      841 acccaacgac gaccatcact ttcggtaaca agtgtatgat cgttgacacc aataatggcg
      901 ttttctgttg tttttagtac catggtgtcc cgccaagttc ggtggagact cccatcatgc
      961 ttataacttt gaattgtaat aaagtcgcct tcttttggaa gcttcataac taaccaactt
     1021 tctacaattt ataagtttat catttactat tgtaccataa aattacctaa aatctgtgaa
     1081 tttcacttgg aaatattaaa gatattctct aagagcgctt gctatatccg aaaaatcgta
     1141 gccctttcgt gctaaaactt gagttaaacg ctgcttcagt tcgtatcctt catactttcg
     1201 ggcatactta gtatattgct tatcaagttc cttgaagatg agttcctgag tcgtttcttg
     1261 gtcaacttga ctatccaatt cgtcaaaggc aattttagca tcaaaataag agaagccctt
     1321 gttagtcaag ttctggataa tcttatcttg cagggcacga gctggaagtt ttccctcata
     1381 ttttttcaat agtttattgg ctacacgttg agcaacttcc gaaaaatcaa aatcattcaa
     1441 gatttcttct atagtagatt ttgaaattcc tttttgtgct aatttctgag tcagtacata
     1501 aggtcccttg tctcctgaaa cttgattggc attgatgata gcataagcgt actgaccatc
     1561 attaatccac ttatcttctt taagattagc aataacttga gaaacgatgt ttttatcaat
     1621 atcgtatttt ttcagatatt ctctgacttc tttttcagtg cgtgctttaa aggataagtg
     1681 gtagagggcc agattcttac cataagaaaa ttgagcaaag tcttgaatct ctttcaattc
     1741 ctcttcgctt atcaccttat ctctcgataa cataaaacga acaattgtat cttcggtgat
     1801 atagcatttg tcgccattat caagctccat cagatagagt ctttttttct tttcaagttt
     1861 tgtgattttc atagctctat tataactcaa aatgtgataa gataggggta tgaatctgaa
     1921 agtgaaacaa aaaataccat taaaaatcaa gcgcatggga attaacggtg agggaatcgg
     1981 cttttaccaa aaaacattag tctttgtacc tggagctctc aaaggcgaag atatctattg
     2041 tcagattact tctattagac gcaactttgt tgaagcaaaa ttactgaagg tcaacaagaa
     2101 gtctaaattt cgaattgtgc catcttgtac tatttataat gaatgcggag gctgccaaat
     2161 catgcacctg cattatgata agcagctgga gttcaagacg gacttacttc atcaagcgct
     2221 gaaaaaattt gctcctgcag gatatgaaaa ttatgaaatt cgtccaacta ttggaatgca
     2281 ggaaccaaaa tattacagag ctaagttaca atttcagact cgaaaattta aaaatcaggt
     2341 caaggcgggc ttatatgcac aaaactctca ctatttagta gagttgaaag actgcctggt
     2401 acaagataag gaaacccaag tgattgctaa tcgcttagca gaattactta cttatcacca
     2461 gattccaatc acggatgaga gaaaagttct aggtgtccgt actattatgg tccgacgcgc
     2521 gagaaagacc ggacaggttc agattattat tgttacaaac cgccagctta atttaactca
     2581 attggtaaaa gagttggtta aagatttccc agaagttgtg acagtagctg ttaatacaaa
     2641 tacagctaaa accagtgaga tatatggtga aaagacagag attatctggg ggcaagagag
     2701 tattcaagaa ggtgtactca attatgaatt ttcactatcc cctcgagctt tttatcaact
     2761 aaatcctgag caaacagaag tcctctatag cgaagcagta aaagcgctgg atgttgataa
     2821 agaagaccat ttgattgacg cttattgtgg agttggaacg attggatttg cctttgcaaa
     2881 gaaagtaaaa acactcagag gtatggatat tattccagaa gctattgaag atgccaagcg
     2941 aaatgctaaa agaatgggat ttgacaatac tcattatgaa gctggaacgg cagaagagat
     3001 tattcctcgt tggtacaagg aaggctaccg agcagatgct ttgattgttg acccaccacg
     3061 tacaggtctg gatgataagt tattagatac tattcttact tatgtaccag aaaaaatggt
     3121 ttatatttct tgtaatgttt cgaccttggc tcgtgatttg gtacgcttag tagaagtcta
     3181 tgatcttcat tatatccagt cggtcgatat gttcccacat acagctcgaa ctgaagctgt
     3241 tgtaaaatta ataaaaaaag ttt
//
LOCUS       DAVLXF010000129         3207 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLXF010000129 DAVLXF010000000
VERSION     DAVLXF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.129"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            99..239
                     /locus_tag="VYO12_001992"
     CDS             99..239
                     /locus_tag="VYO12_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588523.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            276..830
                     /locus_tag="VYO12_001993"
     CDS             276..830
                     /locus_tag="VYO12_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588524.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            845..1996
                     /locus_tag="VYO12_001994"
     CDS             845..1996
                     /locus_tag="VYO12_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1588525.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(2048..2707)
                     /locus_tag="VYO12_001995"
     CDS             complement(2048..2707)
                     /locus_tag="VYO12_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1588526.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            2777..>3207
                     /locus_tag="VYO12_001996"
     CDS             2777..>3207
                     /locus_tag="VYO12_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1588527.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYS"
BASE COUNT         1175 a          474 c          661 g          897 t
ORIGIN      
        1 tactctgaca cagaacttta agaaaaagct aattattgta gtaatttctt taagaaaatg
       61 atataattca ttaaaaagta tttggaggag gaagaattat gttgaatcag gatctctttg
      121 attcgcttga ggcacaaaaa attgtagata ctttgatgaa agggcaaaaa gattatgtag
      181 atgaacgtct agaaaaaaga gagacaatga tagtgtcgaa tggttatgca tggacgtgac
      241 ctaatcatat tgatactgca tttgcatcag cagatttgtt tgagtataaa ttacaattag
      301 caggacagac ttggggatat ttagaatttg aaacaaatac agaaaaatat gggaaagtat
      361 tgttaattat aaagggtaag aagcgactta cgaaccaatt tcctttggta caaaaaaata
      421 agagtggcta cttatttgaa tatgctcaga tgaatacact ttatcttaat caacattctt
      481 cctacaaaaa tgatgaagat agtcattcct ttccaattca gatggagtta gtttctgatg
      541 aaatgattca agaaattgaa caagctacta aaaattcgaa tatcgaaaaa tttatgattt
      601 taacttatga ggcggactca gaaaacaata ttatatctgt agatgttgtt atgcctgatg
      661 cacgaactgg tcagttacac ttgattcaag atttgtctga gtatattcaa tcaagttcgt
      721 accatttcga agaagctaaa tatcaagata ttcctaattt ttcagaatta tcggaaacag
      781 aagattttga aattattcca agaatagaaa aacaagaagg tcaaaagtag taaggaatat
      841 gtaaatgttt aatggtcggg tattgaaaga attacggctg ttaaatggtt taagtagagc
      901 agaattagct cagagaatta atttaacgga acaagccatt tggcagtttg agtccaacga
      961 aacgaaacct aaattatcaa ccaaaatgca tttggccaac caatttcatg ttgatttaac
     1021 ttattttgaa caggaagaag agagcattcg atttgattct tctgtaattg cctttagaaa
     1081 tgcagaccta gcaacacgga aaacaataga tattcaaact atgtatttac ataaggtaga
     1141 tagtttgatt gattattttg aaagttttgt aattatacct aatattacaa ttcatgacct
     1201 aagtaatgta gtgagtgaat cttatcataa gggagaatcc attgaggaat tggctcttta
     1261 tgccagggaa aaattaggta tttcaaaaga taatcatgat ttgctttata aattagaacg
     1321 ttcaggcatc tatatcgtgg aacgattaat taatggccaa gctgatgctt atagcgcatg
     1381 gtcaaaattg ggaagacctt atattgtgtt gggaacgaat aaatcatctg tacgtcgaaa
     1441 ttttgactta gctcatgagc taggacatat tcttttacac aaatataaag atatgaatga
     1501 agatggcaat cgtttggagc aagaagcaaa ttattttgca tcatgttttt tattgccaaa
     1561 agaagagttt ttagtcaaat ttgaagagag ggttggcaag cgtgtcagca atcctgatag
     1621 ttatatttta ttgaagtcgg atttgaatgt ttcgatacag gctttagagt atcgagcttt
     1681 taagttagga ttattgactc caaagcaaca ttcttacttt tatcgtcaaa ttgcgcaaaa
     1741 aggttacaaa atgattgaac ctttggatga tcaaattttt gttaaaaaac caagcaaagt
     1801 aaagagtatt ctggacgtcg ttttgagtaa tcatctagtc agtctagcga ctataatgtc
     1861 taaacaaagt attcgtttac agtttataag cgaaatattt tcagtcgaaa tgaaattttt
     1921 tgatcagtat caagaagata gaagaacaga tcgatttgat aacatcatcc ctttgtacaa
     1981 aagaaataat ttataattta actgtgaaat aatttcatga aggacatatg gcagaaattg
     2041 gattttatca gacaagcagt agcttaccaa aaataatcac acaagcagca agtaagagga
     2101 aaggcacgaa aggaagtctt tcctttttct tttgcaggag aaaggccaga atacccgtcg
     2161 cagaagcgaa ctgaatcaag atcagtaact ccgttacgct aaagacgaga gcacaagaag
     2221 ctaaaaagag gaaatctcct gcgcccatgc ggatatcgat aaaatgagcc aaaattccaa
     2281 gagcaaggaa ggagaccatg accagattcc agccagagga agctattagg attaggtgga
     2341 aagtcatcca gaccagtaag ggatattcct gatggcgaaa gtcgtagatg cctaaggtca
     2401 aaccagcggt gattaggacg acttgcccca aggaaagcaa ttcccaagag taaagcagaa
     2461 agaggagtcc taagactaat tcaaagaggg cataccagac aggatagcga actttgcagt
     2521 agcgacagcg aaagcgattg aagacctgtg agagaatcgg aatcaaatct aagggacgca
     2581 agcgagtctg acaggaatcg cagtgactgg ctgaactgat aatggattgc tctggaaaac
     2641 ggtcaatgac caaaccaaga aaggaagcga gaatgctccc gacgagaaaa aaataaaaat
     2701 caatcatact tatctattcg taaaaaatgg gagaaatagt ataatggaga aacatagata
     2761 agatggtgag gaaaagatga caattcgttt tgaagaaaag gtgagtatag aaaacgctca
     2821 gctcgtatgc caatggtcca actccttagg caaagtcttt caagaacaat ggatgggacc
     2881 aaggattcct tttctactga cccttcaagc cttggaagga gtcttttcaa tctttgatga
     2941 acaagagttt gtgggactta tccagaaaat caggctagaa gacagcaatc ttcatatcgg
     3001 gagatttttt atcaaccccc agaaacagga gcaaggctta ggtagccagg ctttaaggaa
     3061 atttgttagt ttggcctttg aaaatgaaga tatagatagt atttctctaa atgtcttcga
     3121 ggcaaatcaa agagctcaga atctttacca aaaagaagga tttgaaatcg ttcaaatggt
     3181 tgaagcacct gtacgaaaat atagtag
//
LOCUS       DAVLXF010000130         3139 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLXF010000130 DAVLXF010000000
VERSION     DAVLXF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.130"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..218)
                     /locus_tag="VYO12_001997"
     CDS             complement(<1..218)
                     /locus_tag="VYO12_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588528.1"
                     /translation="MFNINDQSIIYVACPYFNKTGGTELVHQLVYSINHFGGKAIVAY
                     YGSESAPNKVNPAFREYVSTFVDIKDVI"
     gene            complement(211..1479)
                     /locus_tag="VYO12_001998"
     CDS             complement(211..1479)
                     /locus_tag="VYO12_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV1588529.1"
                     /translation="MLALTIGTVVFVKKTYRTFLNPIILFVILWFIITTLSSLKLFQL
                     FEISTHTQQIILLGTSLFVIGGGIAIWFRDKFYFKVGGNSYFTTDFEINYNLFFLLGI
                     ICLFYYLPDFFSSLVSLIRGGNLNLVRQSAQDAVDTSGLKNFIGTFIVSPSAMVLEIL
                     GVLDFWSNKKGRKLFYLSLAVIFVRVIADAGRTPLFNVVIYLLLTVLANRFSEKTEKK
                     RKVSKIKIVNYGMLGSIILWLSTLSRTTTSVYRILYFYFAMSPILLEKWSSVLDSEKL
                     VTKGLVSLNGFFFSISYVLKNLFRIDYSQRVLEAYTMIANTDAIWYNIAPGLTKANAY
                     VSLFWFFYADGRLLGVLIGSLLYGTFCGYIFCRYIQQQNKKNLAMLLFIYQGVFFSFI
                     RFPFSKSNYAIAFVLLLFFAFKKKGIEKSV"
     gene            complement(1521..2363)
                     /locus_tag="VYO12_001999"
     CDS             complement(1521..2363)
                     /locus_tag="VYO12_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1588530.1"
                     /translation="MNFCFVILHYRTSNDTIECIKSIQKLEGDYKIVIVDNASQNGSI
                     ERVEALFANNDEIVIIKNTKNLGFAAGNNIGYAYARTQIKADMIAVLNNDIVIKQKDF
                     INRIFELYRNSQFHIAGPDIVSLVDGHHQSPVVEKINSISKANKELFKYRILRIINKI
                     GLYELMTRQPKKELNRISVADPMRYQENVILHGSFVIFSPLYVSEEECAFRPDTFMYM
                     EEPILYEYCMLKNYKTVFDPSIVIYHKEDSSTNSLYNAAYAKREFVFKNMIRSLKIYR
                     SLLK"
     gene            complement(2372..>3139)
                     /locus_tag="VYO12_002000"
     CDS             complement(2372..>3139)
                     /locus_tag="VYO12_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1588531.1"
                     /translation="LFKIPIIEPIYDFLFVEMLNKRTKTVFCKTEKAECYLKNKGFND
                     CKVVGVGLDIEKFEQEEEPTEDTIELLRRMENKQNILYVGSLSKRKNTAQLIRIFNIL
                     KSKSGKKNELQLVLIGKDEDNIVEKINYSRFKDDIIYQPYLKNSQLQFIYPSSQLLVL
                     PSVQEIFGMVLLEAMYFKLPVVSSASAGGETLIQDGINGKIVNDFNDEHWVDCIENLL
                     NNPLELKRLGECAHKRITEQFMWSSIARKIIETFDER"
BASE COUNT         1111 a          579 c          392 g         1057 t
ORIGIN      
        1 tcaataacat cttttatatc aacaaacgta gaaacatatt ctctaaacgc aggattcacc
       61 ttatttggag cactttcact accataataa gcaacgatag ctttcccccc aaaatgatta
      121 atagaataaa ctaattgatg gacaagctcc gttccacctg ttttattaaa ataaggacat
      181 gcaacataaa taatagactg gtcattgata ttaaacactt ttctctattc ctttcttctt
      241 aaaagcaaaa aataataaga gtacgaatgc aattgcataa tttgattttg aaaacggaaa
      301 tctaataaaa gagaaaaaaa caccttgata aataaatagt aacatagcca aattcttttt
      361 attttgttgc tggatatacc tgcaaaagat ataaccacaa aaggtaccat atagaaggga
      421 tcctattaaa actcctagta aacgtccatc cgcataaaag aaccaaaaca gagagacata
      481 tgcatttgcc ttggtcaaac caggtgcaat attataccag attgcatcag tattcgcaat
      541 cattgtgtat gcttctaata ctctttgcga ataatctatt ctaaacagat ttttaagtac
      601 atatgagatt gaaaaaaaga aaccgttcaa cgaaaccaaa ccctttgtta ctaatttttc
      661 agaatcaagt acactactcc acttttctaa taatattggg gacattgcga aataaaaata
      721 aagaatccta taaacggagg ttgttgtcct agataaagtt gatagccata agataattga
      781 acctagcata ccataattta ctatcttaat tttactaact tttctcttct tttcagtttt
      841 ttcagagaac ctatttgcta aaacagtaag taataaatat atgacaacat taaatagagg
      901 tgttctacca gcatctgcaa taactcttac aaaaatgaca gctaaactaa gataaaatag
      961 ttttctacct tttttattgc tccagaaatc taatactcct aatatttcta ataccatggc
     1021 actaggacta acaataaatg ttccaatgaa attctttaat ccactggtat ctactgcatc
     1081 ctgcgcagat tgtctaacta agtttaaatt cccacctctt attaatgata ccagtgagga
     1141 aaagaaatct ggcaaataat agaaaaggca aatgatacct agcaaaaaga acaaattata
     1201 attaatttca aagtctgttg taaaatatga gttaccgcca actttaaaat aaaatttatc
     1261 ccgaaaccaa attgctatcc ctccccctat aacaaataga gaagtcccaa gcaaaataat
     1321 ttgttgtgtg tgagtactaa tctcgaaaag ttggaatagt tttaaacttg acaaagttgt
     1381 tataataaac cacaaaatta caaataaaat tatgggattg agaaacgtcc tatacgtttt
     1441 tttaacaaat accactgttc ctattgtcaa ggctaacata ctaagattca atttctaatt
     1501 attatccttt ccttagttta ctacttcaat agacttctat aaatctttag agatctaatc
     1561 atatttttaa aaacaaactc tctttttgca tatgctgcat tatacaacga atttgttgat
     1621 gagtcctctt tatgataaat tacaatagaa ggatcaaata ctgtcttata atttttaagc
     1681 atgcaatatt catatagaat aggctcttcc atatacatga atgtatccgg tctgaaagca
     1741 cactcctcct ctgaaacata caacggtgaa aaaattacaa aactcccatg taaaataaca
     1801 ttttcctgat atctcatagg gtctgcaaca cttattctat ttaattcttt ttttggctgt
     1861 ctagtcatca gctcatataa tcctatttta ttaataatcc tcaagattct atatttaaaa
     1921 agttctttat tagccttaga tatagaatta atcttctcaa caacaggact ttgatgatga
     1981 ccatctacaa gagagacaat gtctggacca gcgatatgaa actgagaatt tctatagagt
     2041 tcaaatatac gattgatgaa atctttttgc ttaataacaa tatcattatt aagtacagca
     2101 atcatatcag cttttatttg agtcctagcg taggcgtatc caatattatt tccagcagca
     2161 aatcctaagt ttttcgtgtt ttttattata actatctcgt cattatttgc aaataaggct
     2221 tctacccttt ctatactgcc attttgagaa gcattatcaa caattacaat tttataatct
     2281 ccctcaagct tttgaatcga tttgatacac tctatagtgt cattgctggt acgataatgc
     2341 aaaatcacaa aacaaaaatt catagcaagt cttacctttc atcaaatgtc tcaattattt
     2401 ttctcgcaat tgaagaccac ataaactgtt ccgtaatgcg cttatgagca cattctccaa
     2461 gccgtttcaa ttccaatggg ttatttaata gattttctat acaatccacc cagtgttcat
     2521 cattaaaatc attcacaatt ttaccgttta taccatcttg aatcagtgtc tctcctccag
     2581 cacttgcact ggaaacaaca ggcaacttaa aatacattgc ctcaagtaat accataccaa
     2641 aaatctcttg aactgacggg agcacaagta gttgtgatga tgggtaaata aattgaagct
     2701 gagaattctt taaataaggt tgataaataa tatcatcttt aaaccgtgaa tagttaattt
     2761 tttcaacaat attatcctca tccttaccaa tcaataccaa ttgaagttca tttttcttac
     2821 cacttttaga ttttaaaata ttaaaaattc taattaactg agctgtgttt tttctttttg
     2881 atagtgaacc tacataaaga atattttgtt tattctccat tcttcttaaa agctctatag
     2941 tatcctctgt aggttcctct tcttgttcaa acttctcaat atctagacct acgcccacta
     3001 ctttacaatc attgaatcct ttgtttttaa gataacactc tgctttttct gtcttgcaga
     3061 aaactgtttt ggttctctta ttgagcattt ctacaaaaag aaaatcgtat ataggttcaa
     3121 taatgggaat cttaaatag
//
LOCUS       DAVLXF010000131         3097 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLXF010000131 DAVLXF010000000
VERSION     DAVLXF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.131"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..669)
                     /locus_tag="VYO12_002001"
     CDS             complement(<1..669)
                     /locus_tag="VYO12_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1588532.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     GLDVE"
     gene            complement(727..1908)
                     /locus_tag="VYO12_002002"
     CDS             complement(727..1908)
                     /locus_tag="VYO12_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV1588533.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            2091..2570
                     /gene="rlmH"
                     /locus_tag="VYO12_002003"
     CDS             2091..2570
                     /gene="rlmH"
                     /locus_tag="VYO12_002003"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1588534.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEELSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            2614..2687
                     /locus_tag="VYO12_002004"
     tRNA            2614..2687
                     /locus_tag="VYO12_002004"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2648..2650,aa:Arg,seq:tct)
     gene            2853..2978
                     /gene="comC"
                     /locus_tag="VYO12_002005"
     CDS             2853..2978
                     /gene="comC"
                     /locus_tag="VYO12_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1588535.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
BASE COUNT          949 a          499 c          565 g         1084 t
ORIGIN      
        1 ttctacatct aatccgagta gttttcttaa ctgtttttct tcattttgta tgaaataatt
       61 agttttttgc tgtttctttt gttttttctc tgtcagaaga gcttctaact tccttacaga
      121 aatatcttct tctataatcc gttgaaagaa atagtcttgt tgttccttat ttaacccaac
      181 tagggaacgc gcatgggctt gtgatagttt gccattttct acttctgaga gaatctgttc
      241 tggcaaggaa agtaaacgaa tggagttgct gatatatgga cgagacttgc ccatcttatc
      301 tgcaatttca gcatgggtga atcctttctc tacgagagat tcataggcgc gtgcttcttc
      361 tattgggttt aaattttctc tctgtaaatt ttcaataatg gactggacca tcatctcttg
      421 gtctgaaatc tgtttaacaa cagctgggat agaccgtaga ccagctaaaa gtgaagcccg
      481 atagcgtctc tctcctgcaa ggatttcata accaataaca ggagattgac gaacaataat
      541 cggttgaatg accccatttt ctttgataga ctgtgctagt tcatctagtt tttctctatc
      601 aaattctttt cggggttgat agggattttt ttgtatatct gtgatagaaa tcatttcaaa
      661 tttttccatg attctacact aacacatctt ttctcttatg taaagctttc tttacataga
      721 tgtcaattaa gattctaaat cacctgaact cttgttaagt ttgatagagg tagtttcttc
      781 tttcccgtta cgatagtagg ttatcttaat ggtgtctccg atagaatggt tgtaaagagc
      841 actttgtaag tctgttgatg aagcaatctc tttgtcatct acttttgtaa ttacatcgta
      901 tttttcaagg tgaccattgg caggcatatt actttgtacc gaacgaacaa ttacaccaga
      961 tgtaacatta cttggaatat tgagtcttct gatgtcgctt gtactcacat tagataaatt
     1021 aaccatctgg attcccaaag ctggacgcgt cacttttccg tttttttcta actgttcaat
     1081 aatattgata gcatcatttg caggaattgc gaaaccaaga ccttctacag atgttcctcc
     1141 atttgtagca attttacttg aggtaattcc gataacctgc ccttgaatat tgatcagtgg
     1201 gccgccagag ttacctgggt taatagcagt atcagtttgg atggcttttg tagaaatagc
     1261 ttgtccatct tccgatttta aggatacatt tctattgaga ctggatacga taccttgagt
     1321 gacagtattt gcatattcag aacctaacgg gctaccgatg gcaatagcag tttctcctac
     1381 agttaactta ctagaatcac caaactcagc tactgttgtc actttttctg aagagatttt
     1441 gacgacagca atatcagaga aagtgtcagc tccgacaatt tctccaggta ctttagtccc
     1501 atctgacaat cgaatatcta ctttgctggc gccatttata acgtgattgt tggtgacgat
     1561 gtaagcttct ttatcattct ttttataaat aactccagat ccttcactag agattcgctg
     1621 agaatctgtg tcagtatcat cattgccaaa tacgctattt tgtctgtttg ccgaataagt
     1681 aataacagaa acaacagcat cttttacttt gttaacagcc tgtgttgttg aattttcgtt
     1741 cttataggca gtttgtgtaa tagtactatt gttgttagag ttgtttacac tacttttttg
     1801 agttagttga gttattgaaa aactacccaa ggctccacta aaaaagctaa tgacgataac
     1861 gactaataat tgaaaccatt ttttgtaaaa tgtttttaga tgtttcatat ttgcctccat
     1921 atgtttgaat tactgaaagt ataaactgac tagcttaatt ataacttaaa cacaaaagtt
     1981 ttacacaaac tgtggataac tcttttgaaa ctgtgatttt cttaattgaa atctattttt
     2041 tattttgtga ataagatgtg aaaaaataga gaatatgtta gaatagagtc atgaaaatta
     2101 aagttgtaac agttgggaaa ctgaaagaaa agtatttaaa agatggtatc gcagagtatt
     2161 caaaacgaat ttctagattt gctaagtttg aaatgattga gttatcagat gaaaaaacac
     2221 cagataaggc cagtgaatca gaaaatcaaa agattttaga aatagaaggt cagagaattt
     2281 tatcaaaaat tgctgaccgt gatttcgtta ttgtgttagc cattgaaggg aaaactttct
     2341 tctcagaaga attgagtaag cagttagaag aaacttctat aaaaggattt tctactctta
     2401 cttttattat tgggggaagt ttaggattgt catcatctgt aaaaaataga gccaatcttt
     2461 ctgtcagttt tggtcgccta accttgcctc atcagttaat gagactagtt cttgttgaac
     2521 aaatctatcg cgcttttacg attcagcagg gattccccta ccataaatag aaaattgact
     2581 tttaattgaa tttttggtag aataattgtg ttaggtctca tagctcagct ggatagagca
     2641 ttcgccttct aagcgaacgg tcgcaggttc gaatcctgct gggatcaata taatagcaaa
     2701 gctgggaatt ttcccggctt ttttcttaaa aaagtacact ttgggagaaa aaaatgacag
     2761 ttgagagaat tttatctaaa acgaaattcc attttgtata atggtttttg taagttagct
     2821 tacaagaaaa aaacatttta ggagatttta ttatgaaaaa cacagttaaa ttggaacagt
     2881 ttgtagcttt gaaggaaaaa gacttacaaa agattaaagg tggggagatg aggctgtcaa
     2941 aattcttccg tgattttatt ttacaaagaa aaaagtaatg aaataagggg aaagagtaat
     3001 ggatttattt ggatttggga cagttattgt tcatttttta attattagtc acaactacca
     3061 tttaatttgt aaaggtcaaa taaatagaaa agaatta
//
LOCUS       DAVLXF010000132         3096 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLXF010000132 DAVLXF010000000
VERSION     DAVLXF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.132"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            280..1566
                     /locus_tag="VYO12_002006"
     CDS             280..1566
                     /locus_tag="VYO12_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1588536.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     TLIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            1581..1892
                     /locus_tag="VYO12_002007"
     CDS             1581..1892
                     /locus_tag="VYO12_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588537.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2127..2855
                     /locus_tag="VYO12_002008"
                     /pseudo
     CDS             2127..2855
                     /locus_tag="VYO12_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          958 a          538 c          675 g          925 t
ORIGIN      
        1 atccttttcc attataggag caacagagtg ggagtagtca tctaaggact aatttatgta
       61 tttttacgag tcagtatctt tggatactgg tttttacttt tctagacttt ttgactactt
      121 gttaaaactg ggataatttt cgactgttta acagttatta tgcaaagtct aaaagattag
      181 aattgtcaaa acaatccgtc taggcttgat tttatccttt atttactata aaatgagaag
      241 gaaaaatgtc aaacttttat attgcaaata ggagaaatta tgacaaaaac attaaaacgt
      301 cctgaggttt tatcacctgc agggacttta gagaagctaa aggtagctgt tcagtatgga
      361 gcagatgctg tctttatcgg tggtcaggcc tatggtcttc gtagccgtgc gggaaacttt
      421 actttcgaac agatggaaga aggcgtgcag tttgcggcca agtatggtgc caaggtctat
      481 gtagcggcta atatggttat gcacgaagga aatgaagctg gtgctggtga gtggttccgt
      541 aaactgcgtg atatcgggat tgcagcagtt atcgtatctg acccaacctt gattatgatt
      601 gcagcgactg aagcaccagg ccttgaaatc cacctttcta cccaagccag tgccactaac
      661 tatgaaaccc ttgagttctg gaaagagcta ggcttgactc gtgtcgtttt agcgcgtgag
      721 gtttcaatgg aagaattagc tgaaatccgc aaacgtacag atgttgaaat tgaagccttt
      781 gtccatggag ctatgtgtat ttcatactct ggacgttgta ctctttcaaa ccacatgagt
      841 atgcgtgatg ccaaccgtgg tggatgttct cagtcatgcc gttggaaata cgacctttac
      901 gacatgccat ttgggaaaga acgtaagagt ttgcagggtg agattccaga agaattttca
      961 atgtcagccg ttgacatgtc tatgattgac cacattccag atatgattga aaatggtgtg
     1021 gacagtctaa aaatcgaagg acgtatgaag tctattcact acgtatcaac agtaaccaac
     1081 tgctacaagg cggctgtgga tgcctatctt gaaagtcctg aaaagtttga agctatcaaa
     1141 caagacttgg tggacgagat gtggaaggtt gcccaacgtg aactggctac aggattttac
     1201 tatggtacac catctgaaaa tgagcagttg tttggtgctc gccgtaaaat tcctgagtac
     1261 aagtttgtcg ctgaagtggt ttcttatgat gatgcggcac aaacagcaac aattcgtcaa
     1321 cgaaatgtca ttaacgaagg ggaccaagtt gagttttatg gtccaggttt ccgtcatttt
     1381 gaaacctata ttgaagattt gcatgatgcc aaaggcaata aaatcgaccg cgctccaaat
     1441 ccaatggaac tattgactat taaggtgcct caacccgttc aatcaggaga catggttcgt
     1501 gcattaaaag aaggactcat caatctttat aaggaagatg gaaccagcgt cacagttcga
     1561 gcttaagaaa ggaaaaggaa atgatagagg cacagggttt cttagtggat aagcaaacaa
     1621 gatgcattca ttaccatagc aagctggata ttattgcttt acaatgctat gattgtaaaa
     1681 agtattatgc ttgttatcgg tgtcatgatt cattagaaaa tcaccctttt gagccgtatc
     1741 ccttatcttt gatacaggat aagcctattt tatgtggtgt ttgtctaaaa ctactaacat
     1801 ataagcaata taaagaaagc ttaagttgcc ccttttgttt ttctcgcttt aatccaggtt
     1861 gccaaaatca taaggaacgc tattttaaat agcaaatcat ctagttttga agtaggagaa
     1921 aactcaattc caagagaaaa tgaagtaaat cttcccacaa taaaacgcat aatatcaaga
     1981 ttgttcaata cctgatacta tgcgttttta agattttaaa gacttttttc ctttatctgg
     2041 tattttgact acttgttaaa actgggttaa ttttcaactg tttaacagtt attatgcaaa
     2101 gtctaaaaga ttagaattgc gtctagagta tgcgtgatgc caaccgtggt ggatgttctc
     2161 agtcatgccg ttggaaatac gacctttacg atatggcatt tgggaaagaa cgtaagagtt
     2221 tgcagggtga gattccagaa gaattttcaa tgtcagccgt tgacatgtct atgattgacc
     2281 acattccaga tatgattgaa aatggtgtgg acagtctaaa aatcgaagga cgtatgaagt
     2341 ctattcacta cgtatcaaca gtaaccaact gctacaaggc ggctgtggat gcctatcttg
     2401 aaagtcctga aaagtttgaa gctatcaaac aagacttggt ggacgagatg tggaaggttg
     2461 cccaacgtga actggttaca ggattttact atggtacacc atctgaaaat gagcagttgt
     2521 ttggtgctcg tcgtaaaatt cctgagtaca agtttgtcgc tgaagtggtt tcttatgatg
     2581 atgcgacaca aacagcaacc attcgtcaac ggaacgtcat taacgaaggg gaccaagttg
     2641 agttttatgg tccaggtttc cgtcattttg aaacctatat tgaagatttg catgatgcca
     2701 aaggaaataa aatcgaccgc gctccaaatc caatggaact attgactatt aaagtcccac
     2761 aacctgttca agctggagac atggttcgag ctcttaaaga ggggcttatc aatctttata
     2821 aggaagatgg aaccagcgtc acagttcgtg cttaatgtag ttgtttagtt ttaaaaaatt
     2881 atgcaaagct ccatatacaa cacttaaacg agattaaaga atggcgaaat cccctgatgc
     2941 gcaagagatt agctgtcttt tttatttttt aagtgataaa gtcggagctt aggtatcaaa
     3001 gcctatcaaa ttaaacaaag aagcgatgtc ttagatattt tgaaaaaaat taataagcag
     3061 aaaactctct attattttgt tgtatagagt tttttg
//
LOCUS       DAVLXF010000133         3048 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLXF010000133 DAVLXF010000000
VERSION     DAVLXF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.133"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..180
                     /locus_tag="VYO12_002009"
     CDS             <1..180
                     /locus_tag="VYO12_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1588538.1"
                     /translation="YENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGV
                     TLITGKNGTGKSTLL"
     gene            complement(546..1856)
                     /locus_tag="VYO12_002010"
     CDS             complement(546..1856)
                     /locus_tag="VYO12_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1588539.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGKTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2093..2965
                     /locus_tag="VYO12_002011"
     CDS             2093..2965
                     /locus_tag="VYO12_002011"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1588540.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFKRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALDDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          933 a          510 c          697 g          908 t
ORIGIN      
        1 tatgaaaaca gaactgtttc ttttgctatt agttcaaaag gagaaaaaat gaaagtagaa
       61 aatatttcgt atagggtgga tcatcgtata ttgtttgata atatttcttt tgatacttcg
      121 agttcaggcg tgacattaat tactggtaaa aatggtacag gaaagtcaac tttactatag
      181 tagattgaaa ctagaatagt acacatctac ttctaaaata ttgttagaaa tcgatttgac
      241 tatcctgatc tatttgtcct gttcttattt catttcacta catctcaaat tgagtatgac
      301 gaagtgcgct cccatgtcct gggaacgcac tttcttcata tttttcatat tcttgaatcc
      361 atcgataaag actattggga tgaattttta aagttgaact aatcattttt acaggatgag
      421 gtttacagca gagagtttga aggctttatc aaaggttttt cttggcataa tgacttttcc
      481 tcgtttccac ttaattttgt gtctacttta ttataccaag tccaccctta agttagataa
      541 taaatctaac ttaaggaagc tagaaggatg agaatccagg tggtcaagag tcccaaactt
      601 aagctgatgg ggacacccag aataatttgc tttttgaagg caaggccacg ttcctctata
      661 ttgggaagtg agagttgaat gagagaacca gctgatgaaa agggtgagat attagtagat
      721 agagcgccaa taacggtggc tgttgtgagt aagtgaatat caatctgggg attttgagca
      781 ctgatgatag caatgatggg aaagagggct ggagctacaa cggatagggt ggaactaaag
      841 agtgacatca ctccggctat cacacaaaag aacagaggta accagaaatg aggaatggat
      901 gttgtcatga ggtgccctat cagtgtgact aaacctgact tgaccgctag agacattagt
      961 aagctcatgc cgcagagcat gataattgta gcccagggaa ccttagctaa aatggcttct
     1021 tgcttcccta atttgagcct taaggcgagg cagaccatga gtattgagac aaagccaata
     1081 tcaaatgttt tttgataagt agctatccag gcgatgtttg ggaaaatgag atgcaacaag
     1141 ggaaaaagcc aaaccaaaac catgctgctg atcatgagca aggtggtttg tctttgaacc
     1201 ttgctgagga gtggtggttg gtcaatagtc aaggatgagt ttgtttttcc cttactatag
     1261 tgactgtaac aggataataa aagcaagacg atgagtgggt agataatgct gacgataaag
     1321 atatgattgc caagtgaaaa agcttgctct tcccatccca tttgcttaaa caggccttga
     1381 aagacaatgc ctgagctact ggttatcaaa ttagcccctc ctgaagctcc ccaattgacg
     1441 gcttgagctc caatcaaagg gtgtttgtcc gctttttgac agagggtaat cgctagagga
     1501 cagcaaacgg ccatagtagt gaaaaatcca gcacctaaag cagacaaaag ggttgccatc
     1561 aggtataaaa tcatgtagag ggcgttaggg taggtgcgtg tgcggtagag aatgtgttga
     1621 gccaaaacat caagagtacc gttagttatt gcaaagttat aaaagagaga gacgctaaaa
     1681 atggtaaaaa agagtgaggt tggccaaaaa tgaagaagtt ctttggggct taatcccatg
     1741 agagtggttg cgatgaggta agaaaaagca atagccagca ggccaatatt gattttggtg
     1801 cggtaaccaa ttccaatggc tagagcaatg gcgctaatca ttattaaatg aatcattgaa
     1861 ttgtcctttc agttagaata tggaaatagg ttggcaattt tggcgctcaa aagaccacct
     1921 gtttcctatt ggtacttgac tattatacac aaaaaaaata caattgtctt gacaattgta
     1981 ttgacacttg ttagaattaa aaatgaggta ttcaggcttg tgatttccta tggaagttaa
     2041 tagtgattgc ctctaatgct tacaagtgat attaaaaata gaggacttag tgatgtcaat
     2101 catttcaact gatttaaccc cttttcaaat agatgataca ttgaaagcag ccttgcgaga
     2161 agatgttcat tccgaagatt acagtaccaa tgccattttt gatcatcatg gccaagccaa
     2221 ggtgtcgctt tttgccaagg aagctggtgt tttagcgggg ctaaccgttt ttaaaagggt
     2281 ttttacccta tttgatgccg aggtgacctt ccagaatcct catcaattta aggatgggga
     2341 tcgtttgact agtggcgatt tggttttaga aatcataggc tcggtgagaa gtctcttaac
     2401 atgtgaacgc gttgccttga attttttaca acatttatca gggattgctt cgatgacagc
     2461 tgcttatgta gaagccttag acgatgattg cattaaggta tttgatactc gaaaaactac
     2521 tcctaattta cgtctttttg agaaatatgc cgtgagagtt ggcggtggct ataatcatcg
     2581 ctttaattta tcagatgcta tcatgctaaa agacaatcac attgcggcag taggtagtgt
     2641 tcaaaaggca attgctcaag cgcgtgccta tgctcctttt gtgaaaatgg tcgaggtgga
     2701 agtggaaagc cttgctgctg ccgaagaagc tgcggcggcg ggtgctgata ttatcatgtt
     2761 ggataatatg tcattggaac agattgaaca ggccattacc ctaattgcag gacgttctcg
     2821 gattgaatgt tctggaaata ttgatatgac cactattagc cgttttcgtg gtttagcgat
     2881 tgattacgtc tccagtggta gtttaaccca tagtgctaag agtcttgatt tttccatgaa
     2941 ggggttaacc taccttgatg tctaagttgt aaaataaact aactttttaa aggatgtctt
     3001 tcctctagaa cgagttttat gtcagatagt ttaaacgcct cttcaaat
//
LOCUS       DAVLXF010000134         2939 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLXF010000134 DAVLXF010000000
VERSION     DAVLXF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.134"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(67..381)
                     /locus_tag="VYO12_002012"
     CDS             complement(67..381)
                     /locus_tag="VYO12_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEV1588541.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(511..750)
                     /locus_tag="VYO12_002013"
     CDS             complement(511..750)
                     /locus_tag="VYO12_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEV1588542.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(802..2238)
                     /locus_tag="VYO12_002014"
     CDS             complement(802..2238)
                     /locus_tag="VYO12_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1588543.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(2428..2766)
                     /locus_tag="VYO12_002015"
     CDS             complement(2428..2766)
                     /locus_tag="VYO12_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV1588544.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVKEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
BASE COUNT          981 a          606 c          356 g          996 t
ORIGIN      
        1 tcaatttata tcaaactcaa aacaatttat cttaacttaa atatttattg acatttcatg
       61 tgttcatcaa atattctcaa gaatcaaatt agccattttt tcaattccca ttggaatagg
      121 aatataggct tgaggaggta tttgtacaac tggttttcct gctttagaac cagcctcttc
      181 aaattgctta aagtacattt ttgtttgagg gctgacaaga tacaaatcaa aagctgctgc
      241 tgcgatagct ttccctcctt cagtagcact aatagcatca actacaatat ctttcccttt
      301 tccttttaga aactctgttg ttttctgtgc cataagtgat gaagacattc ctgctgcaca
      361 aataattaaa gcttttgcca taatattttc tccttttctt aaatccaatc aaagctgtgc
      421 taagttggct tatttgttat ctatttttat tataaaataa agcgtttcca atgacaattc
      481 cctcattttc ctaaatgata tggaaaaaaa ttatttatac ttcaatttat aaaataaaat
      541 tattcctgag agtagaaatg aaacactatt tgctaaaatc aaaggcaagt ctcctatacg
      601 aataccatga gcaagccaca atgcaatacc aataacttgc ataacataca tacctagagc
      661 aatagatcct gtgtcctttg tcttaactac acgaaaaact tgtggtaaaa atgcaaatgt
      721 tgttaaaatt gctgcaatac ttccaatcat atgtcacctc aatatgctaa acaaactgag
      781 aataatctca gtttgtttat actattctac tgattcaccg ttagatgaaa taacttcctt
      841 ataccagcca aaagattttt tcggggaacg attataactt cccttcccat tatcatcttt
      901 atctacataa ataaaaccat aacgtttccg catttcaccg gtaccagctg aaaccaaatc
      961 aatacatccc catggagtat aacccattaa atcaacacca tcttcaacta cagccttttt
     1021 catttcacga atatgggcac ctagatattc aattctataa tcatcatgta ccataccatc
     1081 tgctgcaact tgatctatag ctccaaaacc attttcaaca ataaagagtg gtaagtgata
     1141 gtggtctgta aaccaattta acgcataacg taaaccttct ggatcaattt gccactccca
     1201 ttcagaagcc ttaacataat tatttttcac taaatcttct gtttcaagat aatcaaaata
     1261 aggattattt tcacgatgag agtctatagc aaaggacata tagtaactga aaccaatgta
     1321 atctacagtc ccaccaagta aatcttcttt atcctggtca gtaaaatcaa ctgaaatacc
     1381 ttttcgttcc caatacttga aaatatgctc aggatattta cctaaaacat gcacatcagc
     1441 aaaataataa cgcttctgca tagctttcat tgccattaag atatccttag gattgcaagt
     1501 aactggataa attggacaca tcgcaatcat acaacctatt tgaaaatctg gattaatctc
     1561 atgaccaatt tttacagctc gtgcagaagc aactaattcg taatgtgctg cttgatacat
     1621 aattgcttct ctattatcac cttcctcata tacaatacct gagttagtaa atggtgcaaa
     1681 atcttcctga taattcgctt gattattgat ttcattgaaa gtcatccaat atttaacctt
     1741 atctttgtaa cgtttaaata cgacttctgc aaaacgagca aagaaatcaa tcaatttcct
     1801 atttttccaa ccaccatatt cggtcactaa gtgataaggc atttcaaaat gagatagagt
     1861 gatgacaggt tcaataccat tctttaagca ttcatcaaaa agattatcat aaaactgtaa
     1921 tccttcttca ttcggctcta actcatcacc ttttggaaag atacgtgtcc atgcaataga
     1981 ggtacggaag cacttgaatc ccatttcagc aaaaagtgct atgtcttctt tataacgatg
     2041 ataaaaatct atcgcctcat gatttggata atatttaccc tctaaaactc ccaaagtaat
     2101 ttcacgagct actccatgac gaccagcagt cataacatca gcaacactaa ttcccttgcc
     2161 accttcttgc catccacctt caagttgatg agcagcaaca gcaccacccc ataaaaatcc
     2221 atctttaaaa gtagtcatct tttttcctcc tgactttgat actcttatta taaaccttaa
     2281 accaaaagat gaaaacgcat tctttttcct tattgttaag gaaagaagta atttttaatg
     2341 gaaatagaac aatatcttct tgtattctcg taatgatatc tttacgattt tcaatacttt
     2401 caaactacaa aaactctcac aataattcta attccctgtg tctataaacg acttatcgct
     2461 ttctggcatc ccagaatcat cttctatata acgttcaact tgcatctgca agtgatattt
     2521 ttttcttaaa tctaagattt tctgcattgt ctttgattga taatgtttat ctaaagtttc
     2581 ttgatttatc cactgatcaa taaggagaat agttccctct ttttcaattg gtaaaaaata
     2641 ttcgtatttc aagttacctt tttgatttct aatttcttta acaaggccac tatcaagcat
     2701 ttctcttgca aactttattg cactatctcc atcaccttta taatatacat gaatagtcaa
     2761 tgtcatctta tatcctccaa aatcatcctt caattttaaa aaaacaagtt tagatgagga
     2821 tctaaacttg ttttttatga actaattatc taacgtttcg ccattacttt caatcacttc
     2881 tttataccaa taaaatgatt ttttcttata gcgatttata gtcaattgaa acaagagca
//
LOCUS       DAVLXF010000135         2914 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLXF010000135 DAVLXF010000000
VERSION     DAVLXF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.135"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            382..858
                     /locus_tag="VYO12_002016"
     CDS             382..858
                     /locus_tag="VYO12_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588545.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            858..1634
                     /locus_tag="VYO12_002017"
     CDS             858..1634
                     /locus_tag="VYO12_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1588546.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQSIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQENGKDSVEYTKDFAGKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYAINPNPARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAAEVLQEYLFGKWSKVEEEMLKVGEERMRDMSKSNGKDA"
     gene            1618..2427
                     /locus_tag="VYO12_002018"
     CDS             1618..2427
                     /locus_tag="VYO12_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HEV1588547.1"
                     /translation="MEKMHSYIKYLTQEDPIITDEGKQIEILHLDIQDDPEIFEDWAK
                     QFRRNYCSDDELAEMIGFMNISPKEYLENFKLPSDSGIGLSTMSGDFGEILVSDYLQY
                     IEEYVVPRTRYDSKVNKNTSTQGSDVLGYKQDSSNVSNDEVVVIEVKSSASDSSSSKA
                     KNKLQEAINHSDKDFERFSTSIVASYLRLKKSNSDQANVVERFLNITDKPFNVIYGAV
                     AVYSNQSYDIDVIKTVVSKNHRDFQKLRLLVIHSNELMKFIRELYSKASEV"
     gene            2424..>2914
                     /locus_tag="VYO12_002019"
     CDS             2424..>2914
                     /locus_tag="VYO12_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence associated protein"
                     /protein_id="HEV1588548.1"
                     /translation="MIEFGRRSLYFSKLVRSKAKMIEFDIPLESHLPISEDAQKSFLG
                     ALAIVADTARKYFEVYINRKSFNLQLKNQLHNAAEYFDALLVSGLGNSAEYQDYIAIL
                     GTTAYYLCDYNGSSRVMINYISDDIQLLEDCMTLIKVFIDVVTDELFLNHTPIEGKYS
                     SEL"
BASE COUNT         1053 a          387 c          564 g          910 t
ORIGIN      
        1 aatatagtta aaatatcagt aaaaacaaca ctttattgag gtattggata cgctttgcta
       61 atagcctaat aatcacatgt ggagtgttgc tacaacgaaa aaggtgataa ttcttgattt
      121 caagctattt tataagcatt ttgtctttgt agataaaggc aattttgaca ataaaaatcc
      181 taaaaggtga atcgttatag atgtatttgt agatatcgtt tgcgcatcga aaaaattaat
      241 acaagaataa atatttatgg ctctttaggt gacttttata gaagtaaagt ttaggataga
      301 aaaacagtca ataacgcacc atttttggtg cgttatgctt ttttatgcta taatggattt
      361 ataaaaataa aggagtttgc catgattgga aaaaacataa aatccttacg taaaacacat
      421 gacttaacac aacacgaatt tgcacgaatt ataggaattt ctcgaaatag cttgagtcgt
      481 tatgaaaatg gaactagttc agtctctacg gaattaattg acatcatttg tcagaagttt
      541 aatgtatctt atgtcgatat tgtaggagaa gataaaatgc tcaatcctgt tgaagattat
      601 gaattgactt tgaaaattga aattgtgaaa gaaagaggtg ctaatctatt atctcgactc
      661 tatcgttatc aagatagtca gggaattagc attgatgatg aatctaatcc ttggatttta
      721 atgagtgatg atctatctga cttgattcag acaaatatct atttagtaga aaattttgat
      781 gaaatagaga gatatagcgg ctatttggat ggaattgaac gtatgttaga gatatctgaa
      841 aagcggatgg tagcttaatg gaaatccaag attatactga tagtgaattc aaacatgctt
      901 tagcaaggaa tcttcgttca ctgacaagag gaaaaaagtc cagtaaacaa tctatagcga
      961 ttttgcttgg aggtcaaagt ggtgccggta agactacaat tcatcgtatt aaacagaaag
     1021 aatttcaagg aaatattgtt atcatagatg gtgatagttt tcgttctcag catccacact
     1081 atttagaact gcagcaagaa aatggcaaag atagcgttga atacaccaaa gattttgcag
     1141 ggaaaatggt agagtcttta gtaaccgaat tgagtcattt gggatacaat cttttgatag
     1201 agggaacttt acgaactatt gatgttccaa agaaaacagc acaactatta aaaaataagg
     1261 ggtatgaggt acaattagcc ttaattgcaa ccaagcccga attgtcctat ctgagcaccc
     1321 ttatccgata cgaagaactg tatgctatca atccaaatcc agcacgcgca actccaaaag
     1381 aacatcatga tttcattgta aatcatctag ttgataatac acgacaattg gaagaactag
     1441 ctatttttga aagaattcaa atttaccaac gagatagaag ttgtgtatat gattcaaaag
     1501 aaaatacaac ttcagcagca gaagtgctac aagaatacct cttcggaaaa tggagcaagg
     1561 tagaggaaga gatgttgaag gtgggagaag aacggatgag ggatatgagt aaaagtaatg
     1621 gaaaagatgc atagttatat aaagtatcta actcaagaag atcctattat tactgatgaa
     1681 ggtaagcaga ttgagattct tcacttagat attcaagatg acccagaaat ttttgaagat
     1741 tgggcaaaac aattcagaag aaattattgt tctgatgatg aacttgctga aatgataggt
     1801 tttatgaata tctcccctaa agagtatctt gaaaatttta aattaccctc ggatagtggt
     1861 attggccttt caacgatgtc aggagatttt ggagaaatat tagtatctga ttatcttcag
     1921 tatattgaag aatatgtggt tcctaggact cgttatgata gtaaagtcaa taaaaatact
     1981 tcaactcaag gaagtgatgt tctaggatat aagcaagatt cttcgaatgt gtcgaatgat
     2041 gaggttgtag ttattgaagt aaaatcatct gcatcagatt ctagtagctc taaagcgaaa
     2101 aataagcttc aagaagctat taatcattct gataaagatt ttgaacgatt ttcaacctca
     2161 attgtagcca gctatttgag gttaaagaaa tctaatagtg atcaagcaaa tgtagtagaa
     2221 agatttttaa acataacgga taaaccgttt aatgtaatct atggagctgt tgcagtctac
     2281 tccaatcaat catatgatat tgatgttatc aagacagtag tttcaaaaaa tcacagagat
     2341 tttcagaaat tacgactatt ggtcattcat tctaatgaat taatgaaatt tattagagaa
     2401 ctctattcga aagcgagtga ggtatgatag aatttgggag aaggtcatta tatttttcaa
     2461 aattagtgcg ttcaaaggca aagatgattg aatttgatat tcctttagaa agtcatttac
     2521 caatttcaga ggatgctcaa aaatcgtttc ttggggcact agcaattgtt gcagatactg
     2581 ctaggaagta ttttgaagtt tatatcaatc gtaagagttt taatttacaa ttaaaaaatc
     2641 aattacacaa tgctgctgaa tattttgatg cattgctggt gtcaggatta ggaaattcag
     2701 ctgaatatca agactatatt gcaatattgg gtaccacagc ttattatcta tgcgattata
     2761 atggtagttc gagagttatg attaactata tatcggatga tatccagtta ttagaagact
     2821 gtatgactct tataaaagta tttattgacg ttgtaacgga tgaactcttt ttgaatcata
     2881 cgccaataga aggtaaatac tctagtgagt taaa
//
LOCUS       DAVLXF010000136         2823 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLXF010000136 DAVLXF010000000
VERSION     DAVLXF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.136"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            24..2003
                     /locus_tag="VYO12_002020"
     CDS             24..2003
                     /locus_tag="VYO12_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1588549.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQRVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            2013..2327
                     /locus_tag="VYO12_002021"
     CDS             2013..2327
                     /locus_tag="VYO12_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEV1588550.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            2358..>2823
                     /locus_tag="VYO12_002022"
     CDS             2358..>2823
                     /locus_tag="VYO12_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588551.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFENLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAY"
BASE COUNT         1010 a          355 c          524 g          934 t
ORIGIN      
        1 gaaaacatac gttttaggag ggaatgaatg tgttaaacat taaagagaaa gatatccttc
       61 aatttttaat aaaaaataag gaacgttttg ttacaagcaa agaactggca gaatatttat
      121 cctgttcaga tagaactgtc cgtaatgtac tgaaattaat tgagaagaca atgattattc
      181 aaagagtccg gttgatttca aaacaaggtc aagggtatca aatatttttt gagaatcaag
      241 gcgcttacca ggagtttaga caaacttatg aactggaaga agactatacg aaaactgcgg
      301 tttcaaaagg tgatgatcgt ttagtattta tcttaaataa attattattt gagcaagttc
      361 cagttctttt tgatgattta gctgatgaac tttacgttag tcgttcaaca ttatctcatg
      421 atttcagaaa gatacgagtg atgttatcag agtataattt gtccattgag agtagggcga
      481 ataaaggagt ttatgtctca ggcgaggaac gtgataaacg acgttttatc ataaattatt
      541 tcctagagaa tcaatttttt aaaacaatac attgctatgt aaaatttaac ttttttgctc
      601 aaactgttcc attagaagaa tttgctagaa ttgttttgga tgaatgccaa gaggccaacc
      661 taaaactatc tgattttgta ctacagaatt tagtagttca catagcattg tctgtgattc
      721 gtttaaaatc cggatttgaa ataaaaaata tagactgtca gatgactgat gatgcaatag
      781 agagaaaggt tgctcaaaga attctttcta aagtaagaga agtaacaaat caggaatttc
      841 cagttcagga aatagactac attactttgc atttgttagc taagagtcaa caatgtcaaa
      901 aaaatcagaa gaatatttct gaagaagttt taaaaaaatc tttatttaaa acatttcaga
      961 atttaggatt agatgatatg tataactttt cttcagattt ccaactaatt gaaggattga
     1021 taactcatct aatgacacta caagtacgtt tagaaagccg gattacctta aataaccctt
     1081 tggtagatga aattaaacag aattatagtg atatattctt tatgactaga gaaattcttg
     1141 caaatatgga caagttcttg gagtggacaa tatcagatga tgaagtagca tatgtaagtc
     1201 ttcacttttt agctgctatg gaacgtagca aagagagtac taagtttaat attcttgcta
     1261 tttgtgcaac aggatttggt gcagcccaaa tgttaagaaa tcgtttggaa acggagtttg
     1321 ggaagcgagt agaagttgtt gatgtaatag gctattatga attgaatcaa gaaaaactta
     1381 aaggaataga ttttatagtg tcagctgttg atttatcaaa tctatatttt caaattccag
     1441 tttttaaagt tagtgtgttt cttaagagtg atgagatgga aatgattaga aaagctatgg
     1501 atcagatgca agtttctagt tatgtacaat catcaaagat taataaattt gaaaacaaca
     1561 gctttagaca gtattttagt aaagaaaatt ttttaatatg tactgaatca gacaaggtaa
     1621 atttacttga aaagatggtt gagagtttat ctgttggtga atcaaatgag tttgaacaat
     1681 ctttacttta tggaattaaa cagcgcgaag aattaagttc agttgttttt tctgaaaaaa
     1741 ttgcagttcc gcatcctatt cagccctttg gtacagaggg aaaggtttcg gttgcaattt
     1801 gtaaagattc gttactctgg gataatcaaa gttcatatgt ccagctagta tttcttctat
     1861 ctccatcaat atatgggaac gaaggtttgg caactgtgac taaaaagata gtctctctga
     1921 cagagaatga tgaattgcaa aatcagctaa tatcttgcaa taactttgaa gattttatta
     1981 acatatttga gaagataaaa taggaggttc atatgaatca agaggaactg caggttgcag
     2041 catttgaaat tattctgcat tcgggaaatg caaggtcaga aatacatgaa gcatttgcaa
     2101 aaatgagaga aggtagtttt gatgatgcag aaagtaaatt aaatcaatca aatgagataa
     2161 ttcttgaagc gcatcatgca caaaccaaac tcttgcaaga atacgcaagt ggagtggaaa
     2221 ttaaaattga aattatcatg gtacatgctc aagaccattt aatgaccact atgacattat
     2281 tagaagtagc aaaggaaatg ttagctcttt ataagaaagt taactaaata tgtgttgtta
     2341 tataaggagg aaatgatatg gataaaaaag aacgacaaaa aattgaacaa caacgtcgag
     2401 agatggctct aactaatact ttttttaatc gatatcttct tctacgttat tcaatagcac
     2461 tcttcttttt tgggaatatt tattggcttt tgaatcagtt tattagtccc tcgcctatta
     2521 ttattttccc cattatgctc atagtatttt ctattttagc aactattgaa caatttaaac
     2581 tttatggaaa taggaaagaa aagttgggaa taacactaat gtttgttaga atacaaatgc
     2641 ttatttcaat aggattactt gttttaactt ggacaagttg gtttgagaat ctttttccga
     2701 tttttgaaaa taaccaagta gcacgtttat ttgtttttgt agttcttcta ttaggtttag
     2761 ttctgagttt gttagacatt agaagaatta aaaaaattta taaacgaaca gataaggctt
     2821 atc
//
LOCUS       DAVLXF010000137         2789 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLXF010000137 DAVLXF010000000
VERSION     DAVLXF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.137"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            64..780
                     /locus_tag="VYO12_002023"
     CDS             64..780
                     /locus_tag="VYO12_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1588552.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKAQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELLKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(820..957)
                     /locus_tag="VYO12_002024"
     CDS             complement(820..957)
                     /locus_tag="VYO12_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588553.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEIADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            1089..1514
                     /gene="rplK"
                     /locus_tag="VYO12_002025"
     CDS             1089..1514
                     /gene="rplK"
                     /locus_tag="VYO12_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1588554.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            1722..2411
                     /gene="rplA"
                     /locus_tag="VYO12_002026"
     CDS             1722..2411
                     /gene="rplA"
                     /locus_tag="VYO12_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1588555.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT          943 a          526 c          623 g          697 t
ORIGIN      
        1 taaaagatta gtcaaacgtt tatcgttaga agtagataaa ctattagaaa taaaggagta
       61 gaaatgaaaa aaagatacct agtcttgaca gctttgctag ccttgagtct agcagcttgt
      121 tcacaagaaa aagcaaaaaa tgaagatgga gcagctaaaa cagaacaaac agctaaagct
      181 gatggaacag taggaagtaa ggctcaagga gctgcccaga aaaaagcaga agtggttaac
      241 aaaggagact actacagcat ccaagggaaa tacgatgaaa tcatcgtagc caataaacac
      301 tatccattgt ctaaagacta caatccaggt gaaaatccga cagccaaggc agaattgctc
      361 aaactcatca aagcaatgca agaggcaggt ttcccgatta gcgatcatta cagtggtttt
      421 agaagttatg aaactcagac caagctctat caagattatg ttaaccaaga tgggaaggca
      481 gcagctgacc gttactctgc ccgacctggc tatagtgagc accagacagg cttggccttt
      541 gatgtgattg ggactgatgg tgatttggtg acagaagaaa aagcagccca atggctcttg
      601 gatcatgcag ctgattatgg ctttgttgtc cgttatctca aaggcaagga aaaggaaaca
      661 ggctatatgg ctgaggaatg gcacctgcgt tatgtaggaa aagaagctaa agaaattgct
      721 gcaagtggtc tcagtttgga agaatactat ggctttgaag gcggagacta cgtcgattaa
      781 tactcttcga aaatctcttc aaaccacgtc agcgtcgcct tacctactga ctgcgtcggt
      841 tctattcaca acctcaaaac agtgttttga gctgacttcg tcagttttat ctgcaatctc
      901 aaagctgtac tttgagcagc ctgcggctag cttcctagtt tgctctttga ttttcattga
      961 gtataaaaaa taaacttttc tcttgcaatt ccagataaat agtgtataat ggatgggtat
     1021 gtgaaaaaca tacttgtggg aggtaaaaat ctctaattac cgccaaaacc acaaaggagg
     1081 atttaaaaat ggctaaaaaa gtcgaaaaac ttgtaaaatt gcaaatccct gctggtaaag
     1141 ctacaccagc tccaccggtt ggacctgctc ttggtcaagc tggtatcaac atcatgggat
     1201 tcacaaaaga gttcaacgct cgtacagctg accaagctgg tatgatcatt ccagttgtta
     1261 tctcagttta cgaagacaaa tcatttactt tcgttacaaa aacaccacca gctgctgttc
     1321 ttttgaaaaa agctgcaggt gttgaaaaag gatcaggtac acctaataaa actaaagttg
     1381 ctacagttac tcgtgcacaa gtacaagaaa ttgcagaaac taagatgcca gatttgaacg
     1441 cagcaaacgt agagtctgca atgcgtatga tcgaaggtac tgctcgttct atgggattca
     1501 ctgttgttga ctaatcaata acacagtaga aaatctcaca gcagtctatt agttgctttt
     1561 catactaggc aagtgactga gccttgtact tgggtacagc aagggagctt aaggccgtag
     1621 gaagagaaaa atagtagact gaaaacccgc aagacttcat catttcgaga agtgacgtgg
     1681 gagatgaaaa tcgattgaac cacttacaag gagaatagaa aatggctaaa aaaagcaaac
     1741 aacttcgtgc tgctcttgag aaaatcgaca gcacaaaagc atacagtgta gaagaagctg
     1801 tagcacttgc aaaagaaact aactttgcaa aatttgacgc aactgtagaa gttgcttaca
     1861 acttgaacat cgacgttaaa aaagctgacc aacaaatccg tggagcaatg gtattgccaa
     1921 acggtactgg taaaacttca cgtgttcttg ttttcgcacg tggtgcaaaa gctgaagaag
     1981 caaaagctgc tggtgcagac tttgttggtg aagatgacct tgttgctaaa atcaacgacg
     2041 gttggttgga cttcgacgta gttatcgcta cacctgatat gatggctctt gttggacgtc
     2101 ttggacgtgt ccttggacca cgtaacttga tgccaaaccc taaaactggt actgtaacaa
     2161 tggatgttgc taaagcagtt gaagagtcta aaggtggtaa aatcacttac cgtgctgacc
     2221 gtgcaggtaa cgttcaagca atcatcggta aagtatcatt tgaagctgaa aaattggttg
     2281 aaaactttaa agctttcaac gaaacaatcc aaaaagcaaa accagctaca gctaaaggaa
     2341 cttacgtaac aaacttgact atcacaacta ctcaaggtgt tggtatcaaa gttgacgtaa
     2401 actcacttta atcagtagtt aaagtaatgt aaaaaagttg aagacactat gtctcaactt
     2461 tttttgatgt acgacgggca tgtcgtatat ctgaggtgta agtcctcggt gggcacctac
     2521 taccggtgaa cccaatagcg attcccaagt ctgactatcg tgaggtagcg gattaaaacg
     2581 gtctggggat agaccgtttt aagtctgacg ctggaaataa gaatcgtcag aggaagggat
     2641 agcgaaatcg tggctctacg aacaggaacg tgataataag gcgtatatag cggataaggt
     2701 atctggctgg ggcgaccact atcaattata ataaaatgaa ataagaatag gacgaatcgt
     2761 tcaggacagt caaatcgatt tctaacaat
//
LOCUS       DAVLXF010000138         2758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLXF010000138 DAVLXF010000000
VERSION     DAVLXF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.138"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(182..387)
                     /locus_tag="VYO12_002027"
                     /pseudo
     CDS             complement(182..387)
                     /locus_tag="VYO12_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            822..1739
                     /locus_tag="VYO12_002028"
     CDS             822..1739
                     /locus_tag="VYO12_002028"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1588556.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(1832..2680)
                     /locus_tag="VYO12_002029"
     CDS             complement(1832..2680)
                     /locus_tag="VYO12_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1588557.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFIFGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
BASE COUNT          889 a          466 c          536 g          867 t
ORIGIN      
        1 atgtatagtg gattgatact cttcgaaaat caaattcaaa ccacgtcagt ttcaccttgc
       61 cgtactcaag tacagcctgc ggctaacttc ctagtttgca ctttaatttt cattgagtat
      121 gaatctggaa tagaacactg ttaaaaattg atttgagttt cctactcgat ttcttcagat
      181 tttatttcaa ttgactataa gaactgctat ctggtttcaa atattggctt gtcgtccctt
      241 cacttgtctg aatggcttga gccgttcctt ctgataaggg aaaagatatt ctttagctga
      301 aacggtctga gaatccaagc taacagatgg agagaaaaat agcccagtct gaagcacact
      361 agatggaagt tgttggaaag cagtcatcta gtttttcttt actgctttgg ctttttttgt
      421 catcagggaa agtgacttgc ttcaaatcag ggagtttagg aaattcttca aacatcttgc
      481 ccaagttgtc tcattttgta aattttacag ccataggtgt acctcgattc taaattctac
      541 cttttcggca ccaattagta atttaaagtt ctcaggagtt agttgcattg ctttttcata
      601 cataagcata gtatgacact ttatggttac cgaacaagtc taatcactcc ttgcaaaatt
      661 ttgaagatgt cctacaagtt tttgagaaga atattgcaaa gttcatagtt aagcaacgat
      721 agaataaagt gctttttgaa aacaggtaca cttcatgtga aaaaaacaac atttcagatt
      781 ttctgttcca gaaatagcta tcgctatgat ataattattt tatgattatt actattccta
      841 taaaaaacca aaaagatatt ggcacaccat ctgattcagt cgttgttctc ggctattttg
      901 atggcataca taaggggcat caagaattat ttcgtgttgc caacaaggct gcgagaaagg
      961 atttattacc tatcgtcgtt atgaccttta atgaatctcc aaagatcgct ttagagcctt
     1021 atcatccaga tttgtttttg catattttga accctgctga acgtgaaaga aaactaaagc
     1081 gcgaaggtgt agaagaatta tatctccttg attttagtag tcaattcgct agtctcactg
     1141 cacaagaatt ctttgcaact tatatcaagg ctatgaatgc caaaattatt gttgcaggtt
     1201 ttgattatac atttggttct gacaaaaaaa cagcagaaga tttaaaggat tactttgatg
     1261 gagaagttat cattgttcca cctgtagaag atgagaaagg aaagattagt tcaactcgta
     1321 tccgtcaagc tattttagat ggaaatgtga aagaagcagg aaaacttttg ggggcaccgc
     1381 ttccatcaag aggtatggta gttcatggta atgctcgtgg tcgtacaatt ggttatccga
     1441 cagcgaattt agtgctttta gaccgtactt atatgccagc agatggcgtt tatgtcgttg
     1501 atgttgagat tcaaagacag aagtatcgtg ctatggctag tgtcgggaaa aatgtgacct
     1561 ttgatggaga agaagcacgt tttgaagtca atatttttga ttttaatcaa gatatttatg
     1621 gggaaaccgt catggtttat tggcttgatc gcattcgtga tatgaccaaa tttgactcag
     1681 ttgaccaatt agtggatcag ttaaaggctg atgaagaagt aactcggaat tggtcttaag
     1741 agcttgagta aataaaacaa aaaagaggtt gtctgtaacc caaaagatag atgatttagt
     1801 ctaacttttg aggtcacgac attacctctt tttattcttt ttcaaaggtg aagccttctc
     1861 ctaggatttc atgggcttct gtaatagtta taaaggcttg aggatcgatt cgatgaatca
     1921 tttccttcgt tttcacaatt tcatttcttc cgacaataca gtagatgatt ttcaaatttt
     1981 ctttactata gtagccttga ccaaaaataa aagtaacacc tcttccgagg tcatcattaa
     2041 tcgccttagc aagttggtca ggacgttttg tgataatcat aaagcctttg ccggcatatc
     2101 ctccttcacc aatcaaatca ataacacgag aaacaataaa atcaaacaaa agcgtgtagg
     2161 aaaccaatct caaatccttg aagattagga gaatcaacat gagaatacaa aaatctaaga
     2221 taaagagcag ttttcctatg gatatatgag tgtatttgtt gagaatacga gctagaatat
     2281 cagttccgcc agttgtacct ccagcattaa aaataattcc aaggccaatt cccaatagga
     2341 ttcccgctat aagggctgtg attagtaaat caccttgaag atcaatatga aggggaatat
     2401 gctcaaaaaa agctaaccag gcggacaaag ctaaggttcc tagtaaacta gaatagaggg
     2461 atttggctcc aaagatcttc caagctagga tgaaaagggg aatattaatc agcaggttca
     2521 tgagggaaac agggatttta aaaagataaa aggtgatgag ggtaatgcct gtcgcccctc
     2581 cttcaaagag atgatgggga actacaaaat aagtcagtcc aaaagcataa atagcagcac
     2641 ctagtaaaat ggtaaaaatg gggtaaattt ttttaatcat aggacacctc ttttcttata
     2701 agttgattat atcaaatttt acggaaaatt tgattgactc cattaggatt tttaatct
//
LOCUS       DAVLXF010000139         2703 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLXF010000139 DAVLXF010000000
VERSION     DAVLXF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.139"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..1226
                     /locus_tag="VYO12_002030"
     CDS             <1..1226
                     /locus_tag="VYO12_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588558.1"
                     /translation="RCWYQTFVSDEASEETPLAERGEKEYIVKSSRMTALCYAESKDG
                     VKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRYKLVSKLEFSPNLHY
                     MVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVITRTWANNVRLSAITF
                     SDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHEGDTLNENYDKVDLE
                     LKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPIIEDDKIWFYYMGGN
                     GNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYDDEIYILADIPENGY
                     IKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLGNSPVAFKFEIENAK
                     LYGIEGSIQQHYLKY"
     gene            1295..>2703
                     /locus_tag="VYO12_002031"
     CDS             1295..>2703
                     /locus_tag="VYO12_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1588559.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPKINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKS"
BASE COUNT          955 a          399 c          520 g          829 t
ORIGIN      
        1 atagatgttg gtatcaaaca tttgttagtg atgaagcaag cgaagagact cctttagcag
       61 aacgtggaga gaaggaatac attgtcaagt catctagaat gacagcactt tgctatgctg
      121 aaagtaaaga tggagtaaaa tgggaaaaac caaatctcaa tctagtaaaa tttaaaggtt
      181 caaaagataa taatattgtt ctgatgcatg cacacggtgc aggtatcatg ctagatgaac
      241 aggagttgga tcctaagaaa cgatataaat tagtttcaaa attagaattt tctccgaatc
      301 ttcattacat ggttgttgtt ttttcagaag acggtattca ttttgacgaa cctattccat
      361 ggaaaaaatg gaatcctgca gcagatagtc ataattttcc atttagagat ccaaaaactg
      421 gtaaatacgc tgttattaca agaacctggg caaataacgt acgtctaagt gcaattactt
      481 tcagtgatga ttttattaat tggtcagaac ctaaagaaat ccttagggga gatggatttg
      541 aagatcaaat ttattctatg ccagtaattt catacgcgga tatctattta ggttttgctt
      601 caatgtacca cgaaggcgac actttaaatg aaaactatga taaagtagac ttagaattga
      661 aattctctgc taatttggaa caatgggata gtgtatgtaa gggcgactat ctcattgaaa
      721 ggggaggtgg agtatacccg cagaatgctg attttgatgc tagttgtatt ttcacggctg
      781 ctccaataat tgaagatgat aagatttggt tctactatat gggtggaaat ggtaatcaca
      841 ctggcttccg agaaagttca ttagctagag gatatattaa gaaagaccaa tttgcttatt
      901 atactaataa gagagaagga attccagcta aaatatctac agttagcttc caaacttacg
      961 atgatgaaat ttatatctta gctgatattc ctgaaaatgg ttatattaaa gttgcggtag
     1021 gtactaaata cggtaatgct tattcagatt ttggtcagga agatatctct cttgttcaag
     1081 tttccgatgg taagtataag ttggaattta ataaaaaatc tatcttagat ttaggtaatt
     1141 ctccagttgc gttcaaattt gaaatcgaga atgcaaaatt gtatggtatt gaaggtagta
     1201 ttcagcaaca ctatctcaaa tattaaccag aataattcgt taacaaataa tctctatatt
     1261 agtggagata gatatataaa atggaggtct tattatggta aagcttaata aggtattaaa
     1321 agggagtgtt gcattcctga gtgcaagttt attgctagca gcttgtggca ataacgctaa
     1381 ttcaaagccc gctgaaaaaa cagctgatgg taaaacgaaa atttcgtttc tatctacatg
     1441 gcaaattaat gatgttcgtc cgcctaaaga tgaaacactt gttggtcaat ggttagaaga
     1501 acagctaggt attgatattg atcttgtaac tattcctggc gagggagtaa gttctaaagt
     1561 aaattctctt attactagta accagttacc gactgttttg ttgacaacag gagataagag
     1621 tgatatcgct ggtatcaaca aacttggtaa acagggtgca ttcttagatt tgtcacaaca
     1681 catggacaaa ctaccaaact ataagaagta ccttgaaaaa aataatgcat tagctcaaat
     1741 agaagatgat gaaaaacata tttatgcttt cacaaaattc tttactgatg agaatatcat
     1801 gtatactaca ccaattctga gaaaagactt acttgttggt tcagaatttg aagatctttc
     1861 aaaaattaaa actgttgatg actatacaaa agttttgaaa tatttgactg aaaaacaagg
     1921 ttctccagca tttattcaac gtaatggcta tgaaggcttc atgaagcgag ttacaccatt
     1981 gtggaattta agccatcgta catactatga ctatgaatca gattcatata aacatccagt
     2041 tgaacaacct caattgaaac aattcgttga atggttgaaa gaattacgta aagataacgt
     2101 acttcaccct gactgggcag ttatgaaaga tgaaacttgg gaaggtctat tggcttctaa
     2161 caagggttca ttcactgttg accgtatgaa tatcattgga gataataact ttgataaatc
     2221 atttgattgg cagccacttg tttatccaaa aattaatgga aaacgttatt tgcaaccgaa
     2281 aacaccgtac atttctgatt caggttgggt aatcaatgcc aatgcaccaa aagaagaaat
     2341 tgataaagcg ttagaattct ttaatttctt atacgctgat gaaaatcaag aaaaacttgc
     2401 tattggattt gaaggtaaaa catttacacg tgaaaaccct aatactcaag caggtattaa
     2461 atggttgatt caaatttacg gtgaaaatgc tggaaatgat aaagcagaac tgctgtttaa
     2521 acatggtaac caagcattta ctcgcgttca aactaaatta gatattgagg cacagccagg
     2581 aacattccct aaagttatgt atgatcaagt agataaaatt aagaaagatt taggtggttt
     2641 tagaccagat actccggttc tttcctttac aactgaagaa ttagatgttt taactaaatc
     2701 tga
//
LOCUS       DAVLXF010000140         2675 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLXF010000140 DAVLXF010000000
VERSION     DAVLXF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.140"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>2675
                     /locus_tag="VYO12_002032"
     CDS             <1..>2675
                     /locus_tag="VYO12_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1588560.1"
                     /translation="IPKGWTNESKQTTNEWLFNFNQEGTIWIWMPKFEISDVDTSYSE
                     APEDIEGQISTVESTFKQRANSLDAGVSRLTEGLRTKADISALNVTAENIRQSVKSLE
                     TDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKMKVGG
                     RNLWIKSKTVGAVIEKLPENHVTGQKECYRLENNSTLTFNLEPDFSSRLYQKVTFSAW
                     IKYENVVQGRNFWNVFNCFKHYLFRKNSETGVQSGPDYATLGMYKGSADWKYITFTYD
                     YSEKTNFDQLKTSLRFNLEGATSGTAWVTGIKVEIGSVATDWSPAPEDADGLITEAKA
                     TFERTAQGLRTDLSAIQEYVNKDGQRQEALQRYTREESTRQATAVRELVNRDFVGKAT
                     YQEDVKGINQRIEAVKTSANKDIASQIASYRQSVDGKFTDISSQITTYKQDVGGQISG
                     LSNRLTSSEQGTTTQISNLSNRINSNKQGTDNQISNLKTQVATNKDNAERQMGRISDQ
                     VSANKANADSQFANVTNQLARKVETTDFQRVKETSKLYERILGNTENGIADKVARMAL
                     TNQLFQVEVGKYSVSGPNLIKNSDFKNATNEWGSTQNLGRLVKHSFYHNGQKDLMRLS
                     NATKNENFLYSHRFNLERNTDYVLNFRGFNNSALASYDVYILGRRAGESDGFTIVKKV
                     VSSKKLSTSRCEDVSVTFNSGEMDNAYIRFDNNGSSSGTADLYITEVDLYKGYKPRTW
                     QPHTEDAVADANKKLEATQTKMTQLAGSWVVENINSAGDIISGINLGANGHNRFVGKL
                     THITGETLIDRAVIKSAMVDKLKTANFEAGSVTTTILEAEAVTAEKLKVDNALIKKLT
                     ATDAFIDQLTSKRIFSIKVESVISSSTFLEAYQGR"
BASE COUNT          896 a          493 c          603 g          683 t
ORIGIN      
        1 attccaaaag gttggacaaa cgaatccaag cagaccacaa atgaatggtt gttcaatttc
       61 aaccaggaag gaaccatttg gatttggatg ccgaagtttg aaataagcga tgtagatact
      121 tcttattcag aagctcctga agatatagaa ggtcagattt caacagtaga atcgaccttc
      181 aaacaacgag ccaactcact cgacgctggt gtaagccgtc tgactgaagg ccttagaacc
      241 aaagccgata tcagcgcact caatgtgact gctgaaaata tccggcaatc ggtgaagagt
      301 cttgagacag acacgcagaa caagctaaat cagaagttga gtcaggctga atttgaggtg
      361 cgagccggct ctatccgtca ggaaatcttg aacgcaacca aggataaagc agataagact
      421 ttagttgtat ctgaagccgg gaaattgcgt gaagaatttt caaaaatgaa ggtgggaggc
      481 cggaatctat ggataaaatc caagacggtt ggagctgtaa ttgaaaaatt acctgaaaac
      541 cacgtcacag gtcaaaaaga atgctatagg ctagagaaca actctacttt aacgttcaac
      601 cttgaaccag atttcagctc aaggttgtac caaaaagtta cttttagcgc ttggatcaag
      661 tacgaaaatg tagttcaagg tcgaaatttt tggaatgtat ttaattgctt caaacattat
      721 ctttttagaa aaaatagtga gaccggagta cagagtggtc cagattatgc tacgcttggt
      781 atgtataaag gttcggcaga ttggaaatat attacattca cttatgacta ctctgaaaaa
      841 acaaattttg atcaattgaa gacatcattg cgattcaatc ttgaaggtgc tacaagcggt
      901 acagcttggg taacaggaat caaggttgaa atcggtagtg tggctacgga ctggagtcct
      961 gcgcctgagg acgctgatgg tctcatcact gaggctaagg ctacctttga gcggacagct
     1021 cagggcttgc gaaccgactt atcagctatt caggaatatg taaataaaga cggtcagcga
     1081 caggaagccc tacagcgcta tactcgtgag gagagcacga gacaagcgac agcagtccgt
     1141 gagctggtca atcgtgattt cgttggtaag gctacttatc aagaagatgt taagggtatc
     1201 aatcagagga ttgaagctgt taaaactagt gcgaataaag acatcgctag tcaaatcgct
     1261 agctatcgtc aatctgtaga tggtaagttc acggatattt caagtcagat aactacttat
     1321 aagcaagatg tgggcggtca aatcagtggt ctatcaaata gacttacaag cagtgagcaa
     1381 ggaaccacta ctcagatttc aaatctttca aatcggataa acagtaataa acaaggcaca
     1441 gataatcaga tttcaaattt aaagactcag gtcgctacaa acaaggataa tgctgaacga
     1501 caaatgggta gaatatctga tcaggtttct gcaaacaaag cgaatgctga tagtcaattt
     1561 gcgaatgtga ccaatcaact agcacgaaaa gtagagacta ctgacttcca gcgtgttaag
     1621 gaaaccagta aactttacga gcggattttg ggcaatactg aaaatggaat tgcggataag
     1681 gttgctcgca tggctctgac caatcaactg tttcaggttg aggttgggaa atatagtgta
     1741 agcggcccta acctcattaa gaatagtgat tttaaaaatg ctacgaatga atggggctca
     1801 actcaaaatt taggaagatt ggttaagcat agcttttatc acaacgggca gaaagacctt
     1861 atgcgtttaa gtaatgcaac taaaaacgaa aactttttgt atagtcaccg ttttaatctt
     1921 gaacgaaata ctgactatgt actgaatttt agaggattta acaacagtgc tctcgcaagc
     1981 tatgatgttt atattttggg acgaagagca ggcgagagcg atggattcac aatcgttaag
     2041 aaagttgtta gcagcaagaa actatctacc tctagatgcg aagatgtctc agtaactttt
     2101 aattccggag aaatggataa tgcttacatt cgttttgata acaatggttc atcatcagga
     2161 acagctgatt tgtatattac agaagttgac ttgtacaaag gttataaacc tagaacatgg
     2221 caaccacata cagaagatgc agtcgcagat gcgaataaga agcttgaagc aacgcaaaca
     2281 aaaatgactc aactagctgg ctcatgggta gttgaaaaca tcaactcggc tggagatatc
     2341 atctctggaa tcaatcttgg cgccaatgga cataaccgct ttgttgggaa attgacccac
     2401 atcactggag agaccctgat tgacagagca gtcatcaagt ctgccatggt tgataagctc
     2461 aaaacggcca attttgaagc tggttcggtc acgactacga tattagaagc tgaagcggta
     2521 actgctgaga agttgaaagt tgacaatgcg cttattaaaa aattaactgc aactgatgct
     2581 tttattgacc aactgacatc taaacgtatc ttctctatta aggttgagtc cgtcatttct
     2641 agctcaacct tcctagaagc ctatcaaggc cgaat
//
LOCUS       DAVLXF010000141         2652 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLXF010000141 DAVLXF010000000
VERSION     DAVLXF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.141"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(105..563)
                     /locus_tag="VYO12_002033"
     CDS             complement(105..563)
                     /locus_tag="VYO12_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1588561.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(560..1000)
                     /locus_tag="VYO12_002034"
     CDS             complement(560..1000)
                     /locus_tag="VYO12_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1588562.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1433..2299
                     /locus_tag="VYO12_002035"
     CDS             1433..2299
                     /locus_tag="VYO12_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588563.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
BASE COUNT          884 a          380 c          567 g          821 t
ORIGIN      
        1 ctgatcgatt tatcctgttc ttatttcatt tcactatagt aaggtgcagc aggataaaga
       61 aaatccgaga agtttctttc tcggatttgc ttattatgag gttcctattt tctatgctcc
      121 aagagttgat tgatgtgatc tactttttta gcctctcttt tatagagaat agcccagatg
      181 atgagataga caatcgcaaa ctcgataatg agctggaggt agaaaatcca gtggaaaggg
      241 aaccaaccag caagagttgc tagtgggaca aagccagcca gcataaggaa gaaatgagtc
      301 agagtggcac gaagcatgct ccagtcacgg ctaaataagc ggttgccaaa gttgaagagc
      361 ataccgatgg ttgcccatat aagtgtgcag tagagcaaga ccagggcacc gtgaacctga
      421 tgctgatcca tcacttggcc gatgagagag tagggattta gtggtgcgta ggtatttggt
      481 gcataaatga gtgaaaagag gatagagagg atgaggccga taagaacacc ggtagctgca
      541 tcgtgaaata cttgtttttt catagttcta atttctcctt gatggttttt agataacggc
      601 gtgaagagta ggtgaagctt tcgtttttca agaaaatttc taccagaccg tttggcgtga
      661 gcttgaggtg agagatggaa tcgatattga tgatttctga ttgggaaatt tggataaaat
      721 tggttggcaa gagtttaaga acctgataga gtcgcaaatc aatgctgtag gtctgactcg
      781 cggtttctgc tagaaccttc cgattctcga tatagaagcg ctgaatcttg ccaatctcaa
      841 ctagatagac ctgatcatcg atttttcctt tgattttttc tctttggtcc agattttctg
      901 cgaactcgat gactttctgg actttttcgg tttcttgagg tgcttggaca atcagctttt
      961 cctcctcgta agtctcacta atctgtagtt ctactttcat agttttctct ccttttcagt
     1021 tatacaaggt tgtgatcact tcctgtatat ccttattaaa cactatttta tagcccatgg
     1081 caaggaacct tgtctatgtg gagggattgg gctcctatgt ggtggagctt ttctgttctt
     1141 tctgaaatat ggtataatag cactaatcaa tttctaggaa aatagatacg gaaaggggct
     1201 gaaagaacta ggggaacagt tggaattaga aagtgatagg tatgaaaagt ggcgttgaag
     1261 ctgaataagt ttataaagcg tttgaatgaa gaaatggaaa gtaaataagt tgacgatgaa
     1321 ttacaaaata gattttatct acaatttgta tctttcaaat cattgatttg aattttagat
     1381 ttaatcatgt tacaattaaa actgaaatca aatagaagag gaaaatgcaa acgtgggcac
     1441 actattagca acaagattaa aaaatagacg gaaagaatta aaaatgtctc agcgagaact
     1501 agctgaaggt atctgtaaac aggggcaaat cagtcgatta gagagcggag agtttacccc
     1561 tggagcggat ttcctgaatg cccttgctaa gaagttaaaa gtcagtatgg attatttttt
     1621 tgatgagcag gttgtagaga aagttgagga gttatcagaa tttaagaaat tagcacagac
     1681 atttattaca aatcgcaatt atgagtcttt gaaatatata tatgaattgg agaatgtaaa
     1741 agctcaccgc ctatctttag cagataaaat ctacatggaa tggataaaat ctctgataga
     1801 tttttatttt tacggacaga aagaagatgc gattgtacga ttagaggggg tattatcaca
     1861 gttaagtgta tctgatttga actatctcca agtttcaaac accctattca atttttatta
     1921 tgatattgga gatttaggtc gtttcaataa aattcgtgaa aaactagagt atcaagtaaa
     1981 tcacctcaat ttgagtacaa tcgaagagtt agaactatct attaagttta actacaacgt
     2041 ttgtcgttat ctatggttac agaaaaatat tgaagaagct attactaaaa ttacagctac
     2101 tataaaacaa tgtaaggctt acagaacgaa ttatcttttg gctgatttgt acttattgat
     2161 gggaaatgta agtgaaaatt tttcttctaa aagttcggtg aaagaatatt ttgagacggc
     2221 atattttcta tacaaacttg aggagaacat gtcaatgact ctgaaagttg agcattacat
     2281 tgcagatata acagaataat gaatacggtg ttcaagtgag catcgttttt caaacctagt
     2341 tcagatacaa aaaagtatca aacaaaaaaa aataaaaaaa cattataaaa acaattgaca
     2401 agctaatttt gaagtgataa attaaatgca agaaaacaaa aggagaggta tatgagaagt
     2461 ttgtttaaga aaatagttgc tgtattagtt attggcttaa ttctgttagg aattgcgaga
     2521 acacctcaag ttcataagat ggctagaggg attgatcctg gtcctgcgaa ttttatttag
     2581 aaatcaattt ctactaattg agtactaatt gagtattaat gggtaaaaag attgtatgga
     2641 taagaaaaat tt
//
LOCUS       DAVLXF010000142         2641 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLXF010000142 DAVLXF010000000
VERSION     DAVLXF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.142"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..871
                     /locus_tag="VYO12_002036"
     CDS             <1..871
                     /locus_tag="VYO12_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1588564.1"
                     /translation="IKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKF
                     DIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQ
                     ILIDGKDIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLAN
                     CTDFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNE
                     KKIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKN
                     LIEKTKMAEEFIY"
     gene            882..1511
                     /locus_tag="VYO12_002037"
     CDS             882..1511
                     /locus_tag="VYO12_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1588565.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYIVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            1412..1969
                     /locus_tag="VYO12_002038"
     CDS             1412..1969
                     /locus_tag="VYO12_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1588566.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            1957..>2641
                     /locus_tag="VYO12_002039"
     CDS             1957..>2641
                     /locus_tag="VYO12_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588567.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLK"
BASE COUNT          969 a          328 c          515 g          829 t
ORIGIN      
        1 gataaaagac tctttagatg catctaaaga gggcttgatg gaaatatttt atttatcgcc
       61 caaaatagaa agattaaaag aaattcaaaa tcaagattta caagaaggcg atgactatag
      121 cttaaaaaaa tttgatattg atctaaaaga tgttgagttt gcctacaata aagacgcaaa
      181 agttttaaat ggtgtaagtt ttaaagctaa gcagggagag gtcactgctt tggtaggtgc
      241 aagtggctgc ggtaaaacaa ctatcttgaa acttatatca agactttatg attatgacaa
      301 gggacaaatc ttaatcgatg gcaaagatat aaaagaaata tcaacagaat ctctttttga
      361 taaggtctct atagttttcc aagatgtggt tctctttaat caaagcgtta tggaaaatat
      421 tagaatcgga aagcaagatg caagtgacga agaggttaaa agagcagcaa aacttgcaaa
      481 ttgcacagat tttatagaaa aaatggataa aggtttcgat acagttattg gtgagaacgg
      541 agctgagcta tcaggaggag aaagacaaag attatcaata gccagagcct tcttaaaaga
      601 tgcgccgata ttgatcttag atgagataac agcaagcctt gatgttaaca acgagaaaaa
      661 gattcaagag tctttaaata atttagttaa agataaaact gttgtaatca tttcacatag
      721 aatgaaatcc atagaaaatg cagacaagat agtagttctt caaaacggaa gagtagaaag
      781 cgaaggtaag catgaagagc ttttacaaaa atcaaaaatt tacaaaaatt taatagaaaa
      841 gacaaaaatg gcagaagaat ttatttatta ggaggactac aatggataat aaaaaattaa
      901 aagtaaaaga tttagtaagt atcggtgttt ttggcgtaat ttattttgcc ttcatgtttg
      961 gagttggtat gatgggcttg attccaatat tgttcttaat atacccgaca gtattagcca
     1021 tagttgcagg aactgttgtt atgttattta tggctaaggt tcaaaagcca tgggcactat
     1081 ttatatttgg tatgatatca ccacttgtga tgtttgcagc tggtcatacc tacatagttg
     1141 tggttttatc acttatagta atgataatag cagaattaat tagaaagatt ggtaattata
     1201 attcatttaa atacaatatg ctttcttatg caatcttcag cacatggata tgtagctctt
     1261 taatgcaaat gcttttagca aaagaaaaat atatggagtg gtctttgatg acgatgggaa
     1321 aagattatgt tgatgtatta gaaaagttaa taacttatcc tcacatggct ttagtagcct
     1381 taggtgcttt cttaggagga attcttggag catatatagg caaggctcta ttgaaaaaac
     1441 acttttcaaa tggattatat tgtgtgggat actttactcc ttgcctaatt ttatggtgtt
     1501 atctgaatta aaccctatag ttaagatgtt tttgagtata cctattgtta ttagaatgtt
     1561 tattttacca tttatggcag caagctttat gataaagacc tcggatgtag gcgcaataat
     1621 ttcatcgatg gataagctta agatttcaaa gaatgtatcc atacctattg cggttatgtt
     1681 tagattcttc ccatctttta aggaggagaa gaaaaacatc aaaatggcta tgagagtaag
     1741 agggataaat tttaaaaacc cagtcaaata tcttgaatat gtttctgtgc cactactcat
     1801 tatatcatct aatatatcag atgacattgc aaaagcggca gaaacaaagg caatagaaaa
     1861 tccaattgcc aagaccagat acattcgcgt aaagatacag ctaattgatt ttgtttatgt
     1921 tttagcggtt gctggactta ttgtgggagg attaatatgg ttgaaataaa aaatttaagt
     1981 cttgattatg gtgaagagca tatattagat gatatatcac tatccatagc cgagggagag
     2041 tgcgtgctat ttacaggaaa aagtggaaat ggtaagtcat ctttaataaa ttcaatcaat
     2101 ggactagctg taaggtatga caacgcaaag acaaagggcg aaataattat tgatggtaag
     2161 aatataaaaa atttggaact ttatcaaatc tcaatgcttg tttcaactgt ttttcaaaat
     2221 cctaagacat atttttttaa tgtcaatacg acattagaat tattatttta tttggaaaat
     2281 atcggtcttg caagagaaga gatggacagg cgtttgaagg atatgcttga gatattcccg
     2341 ataaaaaatc ttttaaacag aaatatattt aatctatccg gcggtgaaaa acaaattctt
     2401 tgcattgcag cttcttatat agcaggtaca aagattatag ttatggatga gccttcatcg
     2461 aatttagata ttaaaagcat aagtgttttg gcaaagatgc taaagatatt aaaagagaaa
     2521 ggcataagca taattgttgc agagcataga atttattatt tgatggacat agttgaccgt
     2581 gtatttttaa tagataaagg aaagcttaaa aaaacttata ctagaagtga atttttaaag
     2641 c
//
LOCUS       DAVLXF010000143         2536 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLXF010000143 DAVLXF010000000
VERSION     DAVLXF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.143"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            82..1143
                     /gene="dinB"
                     /locus_tag="VYO12_002040"
     CDS             82..1143
                     /gene="dinB"
                     /locus_tag="VYO12_002040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1588568.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILCAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSIAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(1290..2135)
                     /locus_tag="VYO12_002041"
     CDS             complement(1290..2135)
                     /locus_tag="VYO12_002041"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1588569.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2390..>2536
                     /locus_tag="VYO12_002042"
     CDS             2390..>2536
                     /locus_tag="VYO12_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1588570.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLN"
BASE COUNT          836 a          508 c          508 g          684 t
ORIGIN      
        1 gctcaaaaca ctgttttgag gttgcagata gagctgacgt ggtttgaaga gattttcgaa
       61 gagtataaaa aggtgctagg catgttgatt tttcctttgt taaatgattt gtcaagaaaa
      121 atcatccata ttgacatgga tgcctttttt gctgcagtgg aaatcaggga taatcctaaa
      181 ctcagaggaa aacctgtcat tattggaagc gaccctcggc aaacaggtgg acggggagtc
      241 gtttctacct gtagttatga ggcaagagct tttggtgtcc attctgccat gagttccaag
      301 gaagcttatg aacgttgtcc ccaggctgtc tttatctcag ggaattatga gaaatacaag
      361 tctgtgggac tccagattcg agctattttt aagcgctata cagatttgat tgaacccatg
      421 agcattgacg aagcctattt ggatgtgaca gaaaataaac tcggtatcaa gtcagcggtc
      481 aaaattgctc gcctcattca aaaagatatc tggcaagaac tccatctaac tgcttctgcc
      541 ggtatttcct ataacaagtt cttagctaaa atggcgagtg attatcaaaa acctcatggt
      601 ttgacagtga ttctacctga acaggctgag gattttctca aacaaatgga tatttccaaa
      661 tttcatggag taggaaaaaa gacagtagaa cgtcttcatc aaatgggcgt ttttactggt
      721 gctgatttac ttgaagttcc tgaggtgacc ctaatagacc gttttggtag actaggctat
      781 gatctgtatc gaaaggctcg tggcattcac aactctccag tcaaatccaa tcgcatccgt
      841 aaatcaatcg gcaaggagaa aacctacggg aagattctct gtgctgagga agatatcaaa
      901 aaagagctga ctcttctatc agaaaaagtc gctctcaatc tacatcaaca agaaaaagct
      961 ggaaaaattg tcattttgaa aatccgctac gaggactttt caactcttac caaacgaaaa
     1021 agtattgctc aaaaaacaca agatgctagt cagataagcc aaatagccct gcaactctat
     1081 gaagaattaa gtgagaaaga aagaggtgtc cgcctattgg ggattaccat gactggattt
     1141 taaagctcaa tgaaaatcaa agagcaaact aggaggctag ccgcaggctg ctcaaaacac
     1201 tgttttgagg ttgcagataa agctgacgcg gtttgaagag attttctaag agtataaaac
     1261 cttgaagagt tgccccttca aggtttttct tatacaaata aacggacaaa actgtaaagt
     1321 agcaaaacac taccaagcac gatacggtat ttaccaaaaa gggtgaagtc gtgttttttc
     1381 acatagctgg tcaagaagcg aatagccacc atgctgaccg caaaagctac tcccatcgcg
     1441 accaagagca aaaacaattg cccaaagctc aagagttctc cggctttcac aaatttgaaa
     1501 atctttaagg cactagctcc aaacataaca ggaatcccaa gatagaaggt aaattctgtc
     1561 acaactgaac gactggttcc atttaacaaa ccaccgacaa tcgttgcacc tgaacggcta
     1621 gtccctggta aaagagcaag aacttggaag agtccgatat agaaagcggt cgtataagga
     1681 agcttgtcca actctgttac acttggctcg atagcacgcg ctttattgcg cttttccaaa
     1741 tagatgaagg caaccccgta gataatcaac atgagagcaa ctgaaaccat gttatggaag
     1801 tgggtatcaa accaatcatc aaatttaaag acaccaagta aaggtaaagt agcaatcaag
     1861 accttcaacc atagtctcca agtcttacga acttcctgtt tgtccttagt cggtttaaaa
     1921 ggattgagct tgttaaaata aatcaccata actgctaaaa tagcaccaag ctgaatcacg
     1981 acattaaaca tggacataaa ggcttcattt tgattttggt attggatgaa ttcctctgct
     2041 aaaatcaagt gacctgtact ggaaatcggc aaccattccg taattccttc aacaatcccg
     2101 aagaagatag attttaaaat ttcaataaga tacatagatt actccctata ttataattag
     2161 cgcagttagc tactgccaaa gcccaagtgg tatacttgga ataagccact gtggattagt
     2221 tcatttcctc caatcgcttg acgatttccg aaaggatcta accacagctt ttgtttcaat
     2281 tggtaatgag tttgataacg catagacgtt ttatctcgtc taaggtaaca aggcaaaaag
     2341 ttcgtggata accaagtaga cttgaaatac tatatctgga ggtactccca tgctttatgt
     2401 gggcattgat atcgctaaaa ataaacacga tgttacagcc ttgaatgttc caggaaaaac
     2461 tgttcttaaa ccactcactt tttcaaataa taaagctggt tttgaactct tagatctgtc
     2521 tcttcgacag ctcaac
//
LOCUS       DAVLXF010000144         2458 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLXF010000144 DAVLXF010000000
VERSION     DAVLXF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.144"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            101..1108
                     /locus_tag="VYO12_002043"
     CDS             101..1108
                     /locus_tag="VYO12_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1588571.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1111..1311
                     /locus_tag="VYO12_002044"
     CDS             1111..1311
                     /locus_tag="VYO12_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1588572.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1463..1645
                     /gene="rpmF"
                     /locus_tag="VYO12_002045"
     CDS             1463..1645
                     /gene="rpmF"
                     /locus_tag="VYO12_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1588573.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1661..1810
                     /gene="rpmG"
                     /locus_tag="VYO12_002046"
     CDS             1661..1810
                     /gene="rpmG"
                     /locus_tag="VYO12_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1588574.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2290..>2458)
                     /locus_tag="VYO12_002047"
     CDS             complement(2290..>2458)
                     /locus_tag="VYO12_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588575.1"
                     /translation="KDKGLWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDG
                     YRVNANGEWVG"
BASE COUNT          809 a          471 c          450 g          728 t
ORIGIN      
        1 ttattgggct gaaagttttt agtgattatt ttcaactttc tattgacttt taaaaacaaa
       61 gtgatatcat ttagatatca aataatggga ggtcattctt atgactgaaa aactaatcaa
      121 ttcaaaacca aatggtgtat tcgcattgat tctcattgag ttgacaatcg tacttggtgt
      181 ctttatattt ataatgggtg ttggttcgga aaacattttt ggaattatta tcggaccttt
      241 actaatcgta attgcaggtc tagctcatgc tggtttaaaa gttgtcaaac ctcaggaagc
      301 tctggttctg acactctttg gtaattatac aggtaccatc aaagaacctg gtttttactt
      361 tgtcaatccc ttcagcgtag cagtcaaccc tgcaaaccac actcgacttg gacaaagtgg
      421 tgatgttagc acaaaatctc cttttttagg agctaaatca tcaaatgaca atgatgtaaa
      481 tcttgaaatt ggcaagaaac acatttccct caaagtcatg accttgagca attctcgtca
      541 aaaaatcaat gattgcttag gaaaccctgt agaaatcggt atcgcggtaa cttggagagt
      601 tgtcgatacc gctaaggcag tcttcaacgt tgataactac aaagaatatc tttcactgca
      661 atgtgatagc gccctccgta atattgtccg catctatcct tacgatgtgt ctcctaatgt
      721 ggatactacg ggtgacgggc aagcagatga aggtagtctc cgtggctcta gcgaaattgt
      781 tgctaaccgt attcgtgaag aaatccaaag tcgtgttgag gatgctggct tggaaatcct
      841 tgaagcacgt atcacttacc tagcttatgc tccagaaatt gctgccgtta tgcttcaacg
      901 ccaacaagca tctgccatta ttgatgcacg taagatgatt gtagatggtg ctgtaggaat
      961 ggttgaaatg gcactagaac ggctcaatga aggggaattg gtagaacttg acgaagaacg
     1021 aaaagctgcc atggtttcaa atctccttgt cgttctttgt ggcaatcatg atgcacaacc
     1081 aattgtcaac acaggaagtc tttactaaag atggctgaag ctaaaaaaaa gcagatcccc
     1141 cttcgactct caacaaagtt atacgctgca ctcgcatcat gggcagaaga cgactttcgt
     1201 tcagtcaatg gccaaatcga atatctcctc acagagtgtg tcaagcaacg gaagaaagac
     1261 ggaaaatacg tatcagaaac cattgacgaa ccatttgaga ttgatattta acaccttctc
     1321 ctgtctgccg tgtcggatag gagaattttt tcatcttttt ttgtcaagta actttacttt
     1381 acaaaaaaaa tgtgttatcc tagtatggtt gatgaaaatc agtagattga atcgaatata
     1441 aatacctaaa ggagaaatca aaatggcagt acctgcacgt cgcacttcaa aagcgaagaa
     1501 aaacaaacgt cgtacacact acaaagtaac agctccatct gtaaactttg acgaaactac
     1561 tggagattac tcacgttctc accgtgtatc acttaaagga tactacaaag gacgtaaaat
     1621 cgctaaagct gcatcagctg aataatagaa gggagatacc atgcgcgtaa atattacact
     1681 tgaacacaaa gaatctggtg aacgcttgta ccttacttct aaaaacaaac gtaacactcc
     1741 agaccgcctt caattgaaga aatactcacc aaaacttcgc aaacacgttg tgtttacaga
     1801 agtgaagtaa gcattcaata cgaatcaata cagaagaaaa acgcttgatc agcgtttttt
     1861 ctttttgtta atttcactat attgcaagag aaatcattcg aatgactaca ttttttgaaa
     1921 taagaatacg gattcggacg attaaaagta agagaaactc aaatgtaagt tggggttcct
     1981 tttttgtata tttttcaaat aacgcctcta cactatttgt agcaaattca ccaactacag
     2041 ttgcatctta gttaaaataa gttagaatat gtaagtgagt accagatata ccaagacatc
     2101 gtcaccatct aaggtatatt caaaatacaa aagttgacca actagatttc tgaatatcct
     2161 tatatatcca ttcttaaaat tggtttaaat agcgtagtct tttaaactag ttttgagaat
     2221 ccaaaaaatc ttcctacata tgtaagaaga ttttttagtt cagaatgatt agatttagct
     2281 aatggatacc tatcctaccc actcaccgtt agcattgact cgatagccat cgggtgtagt
     2341 cgtgtttact aataaatctc ctgaattata ggtatagtac catttaccag aaactgtaaa
     2401 ccaaccagta gccattgaac ctgattcatt taagtaatac cataagcctt tgtcttta
//
LOCUS       DAVLXF010000145         2439 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLXF010000145 DAVLXF010000000
VERSION     DAVLXF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.145"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            79..243
                     /locus_tag="VYO12_002048"
     CDS             79..243
                     /locus_tag="VYO12_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1588576.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            327..893
                     /gene="amaP"
                     /locus_tag="VYO12_002049"
     CDS             327..893
                     /gene="amaP"
                     /locus_tag="VYO12_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1588577.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            905..1075
                     /locus_tag="VYO12_002050"
     CDS             905..1075
                     /locus_tag="VYO12_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1588578.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1106..1714
                     /locus_tag="VYO12_002051"
     CDS             1106..1714
                     /locus_tag="VYO12_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1588579.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1745..1948
                     /locus_tag="VYO12_002052"
     CDS             1745..1948
                     /locus_tag="VYO12_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1588580.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2058..2330)
                     /locus_tag="VYO12_002053"
                     /pseudo
     CDS             complement(2058..2330)
                     /locus_tag="VYO12_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          839 a          374 c          474 g          752 t
ORIGIN      
        1 gtaggcagtg ttgtgatcaa ttattgctgg aggtcttatt ggtctcttgg caggtaaaat
       61 cactaaaaaa gtagttctat gggaatcatc gcaaatgtat tcgctggttt agtcggggca
      121 tatgcaggac aatctctttt aggtagttgg ggtccatcaa tcgctggaat ggctttgctc
      181 ccatctattg taggtgcagc gattgtgatt actgtagtgt cattctttac aggtagaaag
      241 taaacttttc gccagtaaag ttagcaaact atttttaaat caatgacggg aaaaatagtt
      301 taaatgttaa atcgaaagga ttgtatatgt caaaagcaaa gaaaatatgt ttcattattt
      361 tctgtatttt aatcttgaca attttccttc ctgttttgat agattatcat caagttagtg
      421 atctaggtat tcatctactt agctggagac agaactccgt agttgaattc tatcttgcta
      481 gatatgtctt ttgggggaca gtggttctat caactttagt tttattatcc attttagttg
      541 tgatgtttta tcctaaacgt tacttggaaa tccaacttga aactaaaaac gatacattaa
      601 aattaaagaa ttcggcaatc gaaggttttg ttagaagttt ggtgagtgat catagattga
      661 tcaagaaccc aactgttcat gtaaatttac gaaaaaataa atgtttcgtt catgtagaag
      721 gtaaaattct tccttcagac aacatcgctg acagatgcca aataattcaa aatgaaataa
      781 ctaatggatt gaagcagttt tttggtattg agcgtcaagt aaaacttgaa gttgcagtaa
      841 aaaattacca accaaaacct caaaacaaaa agactgttag tcgtgtgaag taaggaagta
      901 aaaaatggaa tggcttaaac aatatcgata tccaattatc gctggtctca taggcgtatt
      961 tctggcttgt ttgattgtct cctttggctt cttcaaaaca atatttgtat tgattttagg
     1021 agcactggga gttgcagctg gattatatat cgaaaaaaac tatatagata aataaaaatt
     1081 actaatttaa ttaaaggagt ttcatatgtc aaacgaaaaa aacacaaata ctaacgtaga
     1141 aaagaaagat gctactgttg tagctcacga aatcaaaggg gaacttactt acgaagataa
     1201 agttatccaa aaaatcattg gtctttcact agaaaacgtt tcaggtcttt tgggaatcga
     1261 tggtggtttc ttctcaaatc ttaaagaaaa aatcgttaac agcgatgacg taacaagtgg
     1321 tgttaacgta gaagttggta aaacacaagt tgcagttgac ttaaacgtta ttgttgagta
     1381 ccaaaaaaat gttccagctt tatattcaga aatcagagaa atcgtatctt cagaagttgc
     1441 taaaatgact gacttggaaa ttgttgaaat caacgtaaac gttgtcgaca tcaaaactaa
     1501 agaacagcat gaagcagact cagtaagcct tcaagatcgc gtatctgacg ttgctgaatc
     1561 aacaggagaa ttcacttcag aacaattcga aaaagctaaa tctggtcttg gatctggttt
     1621 ctcaactgtt caagaaaaag ttagcgaagg tgtagaagct gttaaaggtg cagcaaatgg
     1681 tgtagtatct cacgaaaaca ctcgtgtaaa ctaagataaa ataaatataa caggagaaat
     1741 tatcatgtca gtagaagaaa aattaaatca agctaaaggt tctattaaag aaggtgttgg
     1801 gaaagccatc ggtgatgaaa aaatggaaaa agaaggagca gctgaaaaag ttgtttctaa
     1861 agtaaaagaa gttgccgaag acgctaaaga cgctgtagaa ggtgctgtag aaggtgttaa
     1921 aaacatgttg agtggcgacg ataaataagg ttaaaagtta ctttatcttt ttggtaacat
     1981 tagtcaaaag agtctgagtc aagatgattc tcagaaaaca aaaagctaga gattcccaat
     2041 tgcggaactc tagcttttta attttgcctc tttctcttat tatatttcag caggttgttg
     2101 gccatgagta tgaatcccat gtcaattctc acttgacgct tacctctcag atgacatctc
     2161 ttataaccca aacaaacctt tatctgccca aagacagatt tcatatcaat cttacgttta
     2221 gcgaaaattt gtctaccctt ggaagataaa agtgcctgat attctttagt ttttaaacac
     2281 tggtaacgtt cattcatata cagtcccttt tgaggggctg attcaggttc ataatcgcag
     2341 tcaacattga tttcaaggct gtttgctttc tatctcccct ttttcataat gtatagtaaa
     2401 atgaaataat aacaggacga atcgatcggg acagtcaaa
//
LOCUS       DAVLXF010000146         2393 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLXF010000146 DAVLXF010000000
VERSION     DAVLXF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.146"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(27..740)
                     /locus_tag="VYO12_002054"
     CDS             complement(27..740)
                     /locus_tag="VYO12_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1588581.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1199..1579)
                     /locus_tag="VYO12_002055"
     CDS             complement(1199..1579)
                     /locus_tag="VYO12_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1588582.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            complement(2001..>2393)
                     /locus_tag="VYO12_002056"
     CDS             complement(2001..>2393)
                     /locus_tag="VYO12_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1588583.1"
                     /translation="DHVQRNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKP
                     ESPKPTEEPEEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLK
                     NNLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          710 a          478 c          394 g          811 t
ORIGIN      
        1 tttttgtagt cattagctaa tctcctttaa aatgtgatct tttcaagaat atttttatct
       61 aaacaatcca gcaagtcttg gtaagaatag acttcgtaag tcggctgggc ttgtgtgtga
      121 ttttcgaggt gatgaggatt ataccagata gtgtcaatcc ccgcattatt gccaccttga
      181 atgtcggcgg ttagagaatc tccaatcatc agcgtctttt ctttactaaa tccagcaatt
      241 tgctggccaa tcttttcata aaagagagca tccggctttt gagtttgcaa ctgttctgag
      301 ataaagactt gattgaaata aggtactaga ccagattgag ccaaacgtcc tgtctgaatg
      361 gcagtaatgc catttgtcgc agcatacaag ttataatcac gctcaatgag gctgtccaag
      421 agatcatgag cgcccgatag tgtttgtccc tgctgagcga gataaaattg gtaacgctgg
      481 gcaagaaaac taccgtcttt ttcctgtcca aaatgagaaa ataaacgaga aaagcgcgtg
      541 ttaaccagct cttgtttact gattttcttc agctccaagt ctttccagag agccttgttc
      601 ataggaacgt aataatcttt ataagcctga atatccgcaa ctccttcttc ttttagaagt
      661 tgggtcaaag ccacatcctc agcagcatca aaatcaagaa gagtgtggtc gaggtcgaag
      721 agtagaaatt tgtagaacaa tttgaggttt tcctttctga aaattcatta agaacattat
      781 atcataaagc acctcataca attaactaat ttaatcactt aaaaaaaatt cgaacacttt
      841 ctatacaact gacagctcaa atctttcaga atagaacaat actaactatc gagcaccctg
      901 tcttcataaa tatatgtgta attctagacc tagaattcct ataaactaaa tgctttcata
      961 ctcttccaag taattgattg ccttaaattt taatttttga aggattctaa agctagaata
     1021 gtcccatcac aatcagtttt gattgattca caatttagaa acactatagt ttcactcctg
     1081 ttaaaataaa aaggaactgc ataaagcaat ccctttctga ttttgaaatc atttacttaa
     1141 cattttatag ttgagataat caatagctta cctataaaaa gagttatagt aaaattcctt
     1201 atttattgat ttcaagctcc gctaactgtg tttgaataac tgacagttct gcaccagcct
     1261 gaagaagagc agctgcatta taggcacctt ctacaattgg aaccctgttg atgatgatac
     1321 ttttatcact gaaatcagtc accattttta agttcatttt agcagaacct aggtcaaaaa
     1381 aggcaagtaa agtatctgct ggattttcgg aaacaaccct atctacttga tcaaaactcg
     1441 ttccaattcc tccgccctcg gttcctccta cataagtaat cggaacatct ttagctactt
     1501 tactaatcag ttcaacaaca ccttctgcaa tgtgtttgga atgtgaaacg ataacaagac
     1561 caataccaat actttccatc aaaccacccc agtttctaaa atagcagtaa agagtaatcc
     1621 tgatgagaat gatccaggat caatatgtcc aagaaaccac atgctcctaa gacaagagct
     1681 aacagactgg ccatcaataa tagtattgtt ctttttttca tcattactcc ttaactagtg
     1741 tttaactgat caattagcca gtaaatagtt tatctttatt tacactatct gttaagatat
     1801 agtaaaatga aataagaaca ggacagtcaa atcgatttct aacaatgttt tagaagtaga
     1861 ggtatactat tctagtttca atctactata ttttgcacat tttcatgaaa aaaatgagaa
     1921 ctagaactca cattctgctc tcatttttcg ttttcccgtt ctcctatcct gtttttagga
     1981 gttagaaaat gctgctacct ttacttactc tcctttaata aagccaatag tttttcagct
     2041 tctgccataa tagtattgtt gtcctgggtg ccaaatagta aattattttt taatcctgtg
     2101 agagtctctt tggcattgga cttgataatt ggatcctgga tttttccaag taaatcttca
     2161 gcctctctca gtttttcttc aaccttttca gtctcgacct gaggttcttc tgattcctct
     2221 ggtgattctt cttctggttc ctctgttggt tttggagact ctggtttctc gctttgcggt
     2281 ttctcttctc gaggggtttc ttcctcaggt ttttctgtct gaggtttctc ctcgcttggt
     2341 ttttccgttt gattggtatc agcttgacca tttttgtttc tttgaacatg gtc
//
LOCUS       DAVLXF010000147         2377 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLXF010000147 DAVLXF010000000
VERSION     DAVLXF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.147"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            30..548
                     /locus_tag="VYO12_002057"
     CDS             30..548
                     /locus_tag="VYO12_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1588584.1"
                     /translation="MNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTDKKDAHGIALK
                     LLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPELDKIVGKH
                     SEYTYQLLTRYPNPQKRLEAGFDKLIEIKRLTASNSRAFCRALKSSPNHQIEIRNSLD
                     KTIDFSYSLSFS"
     gene            complement(514..1989)
                     /locus_tag="VYO12_002058"
     CDS             complement(514..1989)
                     /locus_tag="VYO12_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1588585.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVVELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          709 a          547 c          433 g          688 t
ORIGIN      
        1 aagatactgg acattatgcc tttaatctac tgaacttcct gcatgaacaa ggatataaag
       61 tttacaccta cactcctttg ctcatcaagg aatttgccaa atcattatca cttcgcaaga
      121 ctaaaacgga taagaaagac gctcatggca tcgctcttaa acttctttct gaccccaatc
      181 gtgaacaatt tcaacacgat aatcggcaag tagaactaaa aatactggct agacatattc
      241 atcgtctcaa gaaaaaacag tctgattgga aagtacaata cactcgttgt cttgatatca
      301 tctttcctga gttggataaa atcgttggaa agcattcaga atatacctac caactcttga
      361 cacgctaccc taatcctcag aaaaggcttg aggcaggatt tgataagctg atagaaatta
      421 agcgattgac cgcttctaat tcaagggctt tttgccgtgc tctaaaatca tcgccaaatc
      481 atcaaattga aataagaaat agtttagata aaactattga cttttcttat agtttgtctt
      541 tctcttaata acgttgatat gattgacctc tggatcttcc gtcccttgga gggagcaacc
      601 acgttccttg gcgaattgga tatagtctac aatttctcgt gtaatgcgtt caccaggagc
      661 caagatagga atccctggag gatagcacat gacaaattct ccacagacct gtccaacaga
      721 ctcatctaag gtcaaacttt ttctctctga atagaaagct tcttgtggag acagcaccaa
      781 ctcgggctga atatattctc cagctattaa gtccttccca tctcgtgaat agagtctctt
      841 aatatcagcc agagcaccaa ccaagcgctc gatgtcttgg atgcggtcgc cgatggaaat
      901 ataagccaag atattgccga tatcaccaaa ctcgatctga atgtcgtatt cgtctcgcaa
      961 gaggtcataa acctcgatac ctgttaagcc tattccctga gtgtaaactg acaacttggt
     1021 tacatcaaaa tcacagaccg acacaccatc tattaactct tttgagtaag catagtaacc
     1081 gccaatggca ttgatttcat gacgagcgta ctcggatagc tcaacgactt cctcaaacga
     1141 ctctttacca cgaagggcca agttgcgacg tgaaatatcc aaactagcca tcaataagta
     1201 agaggcagat gtagactggg tcaggtttat aatctgacga acgtactcgg gattcatctg
     1261 ctccccgatt aaaagaagcg aactttgtgt caaactccca ccagacttat gcatggaaac
     1321 tgctgccata tcagctcctg catccatagc agaaattgga agtttaccag tgaaatgcaa
     1381 atgcgctcca tgggcttcat ctactaaaac catcatgcca gcttcatgag ccatttctgt
     1441 caaccccttt aggtctgaac agatgccgta gtaagtagga ttgttgatta ggatagcctt
     1501 agcatctgga tgatccttta tggcctgtgc tactcggtca ttttcaagac ctaaagcgat
     1561 accaatctta ggatctacac tcatctcgat atagatggga atggcaccac atagaaccaa
     1621 cgcattgata gcagatttat ggacatttcg tggcagaata atcttatctc ctgccttgca
     1681 ggttgccaga atcatagtct gcaccgatga agttgttcca ccaatcatta gaaaggcatg
     1741 gctagctcca aaagcatctg cagccagctc ctctgcatca cgaataatcg aaataggatg
     1801 gcctaaatta tccaagggtt tcatcgaatt gacatcaatg cctacacatt tttctcctaa
     1861 gagttcgaca agttctggat ttccccgtcc acgcttgtga cctggaacat caaagggaac
     1921 aatccttttc ttgcgtaact tcaccaaggc ctcataaatt ggggcttggt tttgatctaa
     1981 ctctttcaaa acatactcct ttcaatattt ttaggacctt atgaaaacta aggaactaaa
     2041 ggttgactca tgaaaaaaga gcaggttttc acctgctctg caatcttctg acgtgtttat
     2101 tttggtttct gttgagtatg tctgttcccg atgtttccta actctctagt agcaaatatt
     2161 acgtacttta ttgatcgttt cacaagatga cgtgtgcttt caccacacta aaaatttatg
     2221 aattaggaca agtgtcctaa catcaactta taaaagtagg cttttaaccc tatcaataat
     2281 gtggcttttc aaccacgctt cggcattcaa ccctgcctgt ccgtaggcat tttttactcg
     2341 ggtttatatt tgctagaaaa catcatctca ttttcta
//
LOCUS       DAVLXF010000148         2227 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLXF010000148 DAVLXF010000000
VERSION     DAVLXF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.148"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            14..1768
                     /locus_tag="VYO12_002059"
     CDS             14..1768
                     /locus_tag="VYO12_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588586.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNEGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1734..>2227
                     /locus_tag="VYO12_002060"
     CDS             1734..>2227
                     /locus_tag="VYO12_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588587.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMG"
BASE COUNT          777 a          308 c          421 g          721 t
ORIGIN      
        1 aggagatatg cttatgaaga tttacaaaaa actatttgct tatgtccaag ataagaaata
       61 tcttggggtt ttggccataa ttttttctgc tatatctgct ctacttacag tatatggata
      121 ttatttaatc tacaaatttc tagataagtt aataattaat tcaaatttat ccggtgcaga
      181 gagtatagca ttaaaatctg ttattacact aacaagtgga gcgatatttt attttgtctc
      241 aggaatgttt tcacatatct tgggattcag gcttgaaaca aatttaagaa aaaggggaat
      301 cgatggtctg gaaaaagcaa gttttaggtt ctttgactta aatccatctg gtcaaataag
      361 aaagatcata gatgataatg cagcacaaac tcatcaggtg gtagcacaca tgattcccga
      421 tagttctcag gcaataatca cacccgtact tgtacttgca cttggcttta tagtaagtat
      481 aagagttggc ataattttgc ttgctcttac tataattggt ggcttaattt taggggcgat
      541 gatgggcgag caagaattta tgaagatata ccaagaatcc ctatctaaac taagtgctga
      601 aactgttgag tacgtgagag gaatgcaagt tgtaaaaata tttaaagcaa atgtagagtc
      661 atttaaaagc ttttacaagg ctataaaaga ttactcaaag tatgcttatg attattccct
      721 atcttgtaaa aggccttatg ttttgtatca atggttattt tttggactga ttgcaatttt
      781 aattattcct atagtttatt ttatgactag cttagctagc gcaaaggtga ttttacttga
      841 gcttatcatg attttatttt tatcaggagt tctctttgtt tcattcatga gaatgatgtg
      901 gtactccatg tatatttctc aaggaaatta tgcagtagat actttagagg cgctttacga
      961 agatatgcaa aaagacaaat tagtgcatgg taatgtcaat aattttaaaa actataatat
     1021 agaatttgag aatgttagct ttgcttataa tgataaagct gtcattgaaa atttatcctt
     1081 taatttagaa gaaggaaagt cctacgcact tgtcggttca tctggatcag gcaaatcaac
     1141 agtagcaaaa cttatatcag gtttttacaa tgttaatgaa ggaagcataa agataggcgg
     1201 gatagcaata agtgaatatt ctgacgaagc cttaattaaa gccatttcct ttgtttttca
     1261 agattcaaaa ttattcaaga agagcattta tgataatgta gctttagcta ataaagatgc
     1321 gacgaaagat gacgttatga gagccttaaa attagcagga tgcgatttaa tattagacaa
     1381 attcccagaa agagaaaata caatcatagg ctcaaaaggt gtttatttat ccggtggaga
     1441 aaaacaaaga attgcaattg ctagagcaat tttaaaggat tccaaaatta ttattatgga
     1501 tgaagcatca gcatctattg acccagataa cgagtttgaa ttgcaaaaag cttttaaaaa
     1561 tcttatgaag gataaaacag ttatcatgat tgcacacagg ctatctacaa ttaaagacct
     1621 tgatgaaatt attgtcatgg atagtggaaa aattatagaa agagggtctg acaaagaatt
     1681 aatgtcaaaa gatacaaggt ataagagcct gcaagagatg tttaacagtg cgaatgaatg
     1741 gagggtttca aatgaaagag ttttataaaa aaagatttgc tcttacagat ggaggagcaa
     1801 gaaatttaag taaagcaaca ctggcttcat ttttcgttta ttgtataaac atgcttcctg
     1861 ccatattact tatgattttt gctcaggaag ttttggaaaa tatgggcaaa agcaatggct
     1921 tttatatagt attctcagtt ttgactttga tagcaatgta tattttgctt tctatcgaat
     1981 acgataaatt atataacaca acctatcaag aaagtgcaga tttaagaata aggacagcgg
     2041 agaatttatc aaaattacct ctatcttact tttctaaaca tgacatttcc gacatttcac
     2101 aaacaatcat ggctgatatt gaaggcatag agcatgcaat gagccactca ataccaaagg
     2161 tgggcggcat ggtactgttt ttcccattaa tatctgtaat gatgctagcg ggcaatgtca
     2221 agatggg
//
LOCUS       DAVLXF010000149         2160 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLXF010000149 DAVLXF010000000
VERSION     DAVLXF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.149"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            329..568
                     /locus_tag="VYO12_002061"
     CDS             329..568
                     /locus_tag="VYO12_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588588.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            553..831
                     /locus_tag="VYO12_002062"
     CDS             553..831
                     /locus_tag="VYO12_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588589.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            1120..>2160
                     /locus_tag="VYO12_002063"
     CDS             1120..>2160
                     /locus_tag="VYO12_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV1588590.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVAKQSQAER
                     DYDAAMKKSEAAKKEYEEAKKDLEEAKAAQKKYEDDQKKTEEKAKLVQKADEERQKAN
                     VAVQKAYLKLREAQEQLNKSPNNKKNAAQQKLKDALAHIDEVTLKQKEAEANFNKEQA
                     KVVPEADKLAETKKKAEQAEKKEPELAKKVAEAKAKAEEAEKKAVEAKQKVDAEKYAL
                     EAKIAELEYEVQGLEKELKEIDESDSEDYIKEGLRAPLQSKLDAKKAKLSKLEELSDK
                     IDELDAEIAKLEKDVEDFKNSDGEQAEQYLVAAKKDLDAKKAELENTEADLKKAVDEP
                     ETPAPAPKPAPAP"
BASE COUNT          840 a          308 c          434 g          578 t
ORIGIN      
        1 taaaagtaat atagtagatt gaatctggaa tagtccgtta tgactgttaa aatatttcta
       61 gaaattaatt tgactttcct aatcgatttg ttcacatctt gtttcaatct actatacttt
      121 ctcgttaaac cgttcggcaa ttcatggtga catggacaca tagtagtaag cacactatgt
      181 ggcttggcag agctaaaaac tgttcccttg cgataagcct aataagcaca acatagggag
      241 ttagagaagc cgactctaat caaccacttt gggcagtagt gagaactgcc ccgtgctttt
      301 ttattttgag aaaatatgga gtttgtcgtt gaaattactt gattgtattt tggactatca
      361 agaaagattc aatggaaaaa catgtcaagt atcaacgaat tataagtatt tagagatttt
      421 caaagtgaat ttttgcttga ctgatttaca tcatttattt gacttataca aaatcacacg
      481 agattatgct agtcaaacaa aacctgctat tcaagctggt gtttttattt tagaagattt
      541 tagaaatatc ctatgtacaa tgatgtaatc gaaaggatat ctttatatga gtttatcggt
      601 gatattttct attctaaaat aataagttgt tgcatcgtag ctagggattt atctaaaaat
      661 actatgaaat tggacgtcat attttttgag gataaaaata aaagatccgc agttttaggt
      721 ttacgaagag acaaaagcgg agtatttaaa tcagttactc tacattttac aagcgctaag
      781 aaatatgcta aagttcgtaa aacagatgtg aaagaaatga aatggctata aagattgtcc
      841 gcaggcttaa gcttgcgttt tttgttgttt aacgaaatta aaaaacaagc tctctcatcg
      901 gaaatgtttt tataggaatg aaggaagatg atgctatgtt taaaaagaga atcgaaaagg
      961 gcaaaagtag tatctttatc tattttttgg agtagaagct tatgatatag aaatttgtaa
     1021 taaaaatgta atataaaaca tttgacaaat atttacgggg ggagtatact taatataagt
     1081 atagtctaaa aatgactatc agaaaagagg taaatttaga tgaataagaa aaaaatgatt
     1141 ttaacaagtc tagccagcgt cgctatctta ggggctggtt ttgttgcgtc tcagcctact
     1201 gttgtaagag cagaagaagc tcccgtagct aagcagtctc aagctgagag agactatgat
     1261 gcagcaatga aaaaatctga agctgctaag aaagaatacg aagaagctaa gaaagattta
     1321 gaagaagcaa aagctgctca gaaaaaatat gaagacgatc aaaagaaaac tgaagagaaa
     1381 gcaaagcttg ttcaaaaagc ggatgaagag cgtcaaaaag cgaatgtggc agttcaaaag
     1441 gcgtatttaa aactccgaga agcacaagaa caattgaaca aaagtccaaa caataagaaa
     1501 aatgccgctc aacagaaatt aaaagatgca ttagctcata ttgatgaggt aacattaaaa
     1561 caaaaagagg cggaagcgaa tttcaataag gaacaagcaa aagtagttcc tgaagcagat
     1621 aagttagctg agactaagaa aaaagcagaa caggctgaaa aaaaagaacc agaacttgct
     1681 aaaaaagtag cagaagctaa agcaaaagca gaagaagctg agaaaaaagc tgttgaagcc
     1741 aaacaaaaag tggatgctga aaaatatgct cttgaagcta aaatcgctga gttggaatat
     1801 gaagttcagg gactagaaaa agagctcaaa gagattgatg agtctgactc agaagattat
     1861 attaaagaag gtctccgtgc tcctcttcaa tctaaattgg atgccaaaaa agctaaacta
     1921 tcaaaacttg aagagttgag tgataagatt gatgagttag acgctgaaat tgcaaaactt
     1981 gaaaaagatg tagaagattt caaaaactca gacggtgagc aagctgaaca atatcttgtt
     2041 gcagctaaaa aagacttaga tgctaaaaaa gctgaattag aaaatactga agctgacctt
     2101 aagaaagcag ttgatgagcc agaaactcca gctccggctc ctaaaccagc cccagctcca
//
LOCUS       DAVLXF010000150         2127 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLXF010000150 DAVLXF010000000
VERSION     DAVLXF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.150"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            256..1659
                     /locus_tag="VYO12_002064"
     CDS             256..1659
                     /locus_tag="VYO12_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV1588591.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVVIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYWYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
     gene            1848..>2127
                     /locus_tag="VYO12_002065"
     CDS             1848..>2127
                     /locus_tag="VYO12_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1588592.1"
                     /translation="MKDVVYSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLIN
                     GSLKPQKGYVNLLSHGKKSSDSIPTVDQTPYIFDTTIRENVTLFQNEYF"
BASE COUNT          768 a          272 c          412 g          675 t
ORIGIN      
        1 agttatggat ctcgttccaa tatgtgcacg ttggaatgtc agtgcttata tggggggaat
       61 ccaaaggatt ctcccttttt attttaataa gtctgtctag ctatagatat attaagtttg
      121 atagaatttc ttggagattt tgttatggat tataatttca atttggagca tcctttcttt
      181 ttcactaata atgattatag tacagatact agcataaagt actaagtaag tctgcctttt
      241 aattggcacg aagtaatgaa taatgatgag tgggtttatc aataccctat tggaaaattt
      301 gttgaaagac aagggtggaa aatacacatt agttcagagt ataatagttc acacgaattg
      361 ttacaagatg tagcaaaaat ttgccatgaa atgcgaattc catttaaaca tcttagtaca
      421 gaagacaaat ttatcatgcg aaacgggaaa ttggttagtc gaggattttc tggtaaattt
      481 attacatgtt atccaaatca aaatgaatta gagtcggttt tgcaaaggtt agaaagtgcc
      541 ttaaagcaat ataatgggcc atatatttta agcgataaac gttgggatga agcaccaata
      601 tatctgaggt atggtgtgtt taggccgtca agggatgatg aaaagaaggt tgttattgat
      661 gaattaattg ttggcgatga agttgttaaa gatgaacgtt taccagtatt taaaattcca
      721 aaaggtatag tgccccctga ctttttgaat aaatggcttg acaagaaaga taaaaaacag
      781 ggagactttc cctttattat agataatgca attagatttt caaacagtgg tgggatatat
      841 aatgctagac taaaagaaga tgggaaaaaa ataattttaa aagaggctag accgtataca
      901 ggtctaggat ttgatggtac ttattcttca gagagattag cttcggagtg caaagcacta
      961 aaaattctaa atgaatggag tgagatgcct aaaatatact ggtacggtaa gatctgggag
     1021 cacaccttct taggaattga acatatgaag ggcgttccac taaatagatg ggttaccaat
     1081 aattttcctt tatatgaagt tgttgataag accaaagatt atctattgag agtttcaaag
     1141 atagtagaaa aactcatcga tttaacgaat aagtttcatt ctgaaaatgt atatcatcaa
     1201 gatttacatt taggaaatat cttggttaag gatgaagatg agattagtat aatagactgg
     1261 gaacaggctg tcttttcgaa tgatgaaaaa gttgtacata aagttgcagc ccctggtttt
     1321 agagcatggc gagaaacact tcccagtgag atagattggt atggtattag acaaatagct
     1381 cattatttat acatgccact agtaactaca tcggatttga catacaacta tgtaagtcaa
     1441 accaggatag aaggaaaaaa actatttgag tctttgggtt atacaagaga acatatagat
     1501 tatgtagaaa gtttattgtc atatctagat tctaaatgtc cacagattga gaatatttcc
     1561 aggaaaaaag ttttaaaacc catgcatgag attagaacta tcgagagtga gcaagatata
     1621 caagatttta ttataaaagc ttttctttta gtgaactgat aggtattttc ttagcaaacg
     1681 ataaagtttt agggccgata caatcaattg cctattcatt aaataagata aatacaacca
     1741 aagatttaag gaaaccgttt ttaaaatact taagtggaga gaagaatttt atagacgctg
     1801 aacatgataa taacggactg tatacttcat caatagatgt gatacacatg aaagatgttg
     1861 tatattctat tacaccagaa aataaattaa gtattgactt ctcatttaag tcaccattta
     1921 gggtattatt aacaggaact tctggtagtg ggaaaacaac gattttaaat ttaattaatg
     1981 gttctttaaa gccacaaaaa ggttatgtaa atttgttatc acatgggaaa aagagttcag
     2041 attcaatacc aacagttgat cagacaccat atatttttga cactactatt cgtgagaacg
     2101 taactttatt tcaaaatgaa tattttt
//
LOCUS       DAVLXF010000151         2070 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLXF010000151 DAVLXF010000000
VERSION     DAVLXF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.151"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(16..249)
                     /locus_tag="VYO12_002066"
     CDS             complement(16..249)
                     /locus_tag="VYO12_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1588593.1"
                     /translation="MEIKYVSVENSVAEWADKKSILKRWEGLNLYTLNRWIKEMRENK
                     RFSLYVINPTHKLVFINLEGFECFLRWKQKRTK"
     gene            complement(551..772)
                     /locus_tag="VYO12_002067"
     CDS             complement(551..772)
                     /locus_tag="VYO12_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588594.1"
                     /translation="MYRRLRDLREDHDLTQKQIATILSFTNSAYAKIERSEHALTADV
                     LVKLSDFYDVSTDYLLGLTDFPDKIRFRK"
     gene            complement(1029..1502)
                     /gene="tnpA"
                     /locus_tag="VYO12_002068"
     CDS             complement(1029..1502)
                     /gene="tnpA"
                     /locus_tag="VYO12_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEV1588595.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            complement(1660..>2070)
                     /locus_tag="VYO12_002069"
     CDS             complement(1660..>2070)
                     /locus_tag="VYO12_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1588596.1"
                     /translation="EIIIYIITLAFEINIAMDRFFYTVLLSIVVLLFWDSLAILLTMF
                     INDYRRRDIVIRFVLTPLGFTAPVFYLIDSAPSIVRWIGQLNPLTYQLTILRNFYFKN
                     STTLELVFLLLTSLLVLISVSFIIPKIKLILIER"
BASE COUNT          653 a          416 c          327 g          674 t
ORIGIN      
        1 tataccatga cttccttatt ttgtccgctt ttgtttccat ctcaaaaagc attcgaatcc
       61 ctcaagattt ataaacacca gcttatgtgt aggattgatg acgtataaag aaaatctctt
      121 attttctctc atttctttga tccatctgtt aagagtgtag agattcaaac cttcccatcg
      181 tttcagtatg cttttcttat ccgcccactc agctaccgaa ttttcaacag atacgtattt
      241 tatctccata ttatcatcct ttcgaataat ttcttctccc tccatggcat gactgatggg
      301 gtcatcataa gaatctcact tactgctctt ttacgacggc agcctgaacg ttcagaagta
      361 tcattgtata tcatttgcat catggcagat agagtatcgc tctattttat tggtgattgt
      421 ccccgctcct atcttggcga atcaagcaaa actgctccac aaatgaggaa agtaccattt
      481 taggctattc agttgtcaaa gggcaaataa aaaatctcac tcattttcaa actctatgaa
      541 attgaggaga ttattttcta aagcgaattt tatcaggaaa atcagttaat cccaataaat
      601 agtctgtact gacgtcatag aaatctgaga gtttaaccaa tacatcagcc gtcaacgcat
      661 gctcactccg ttcaattttg gcataagctg aatttgtaaa cgaaagtatt gtagctattt
      721 gcttttgggt cagatcatga tcctccctca aatctctcaa acgtctgtac atctagcttt
      781 ctcctaagaa aaagtataga ccatatattg taactattta ggatattgtc caaatcggac
      841 aacaggaatt ttaccatcct ttatatagac ttttattttt atgtatcaag ttttttaaat
      901 caaaaccacc agtgtgaact ggtggtttgt tctgcggcta taagccgctt ctaccggcca
      961 gggccaaagg cccaccgaaa tagcttcctc gcgcaccact ttcccgagca ggtgctaaag
     1021 caccttagtt acttcctctt atttatttcg ccagtaaacg gatctactga ctcgaataac
     1081 gtgagctggt ctgctactct gtcttcttgt aattgattct gaatatattc agctatcact
     1141 ttctgattac ggcctaccgt atctacataa tagcctctac accaaaactt gcgattgcca
     1201 tatttgtatt ttaaattcgc atgcttatca aaaatcatca aactgctctt gccctttaaa
     1261 tagcccataa aggacgaaac actaagtttc ggaggaatac tgataagcat gtgaatatgg
     1321 tctgaacaag cattcgcttc atggattatt acacccttac gctcacataa gtcacgtatg
     1381 attcttccga tactagcttt gtatctgcca taaatgattt gacgacgata tttgggtgca
     1441 aaaacaatat gatatttaca attccatgtg gtatgtgata aactttgatt atcctctctc
     1501 atgaggtacc tcctgtatga tatgttgtag tggcggagaa accacttcta tcttatcatt
     1561 ttaggaggtt ctttttgtta ccacgctaaa agctctatgg aaccactagc atagctagtg
     1621 gttttcggga gacaacaaaa aagttggatg acccaacttt tatctttcta tcagtatcaa
     1681 ttttatcttt ggtataataa aagatacaga tataaggaca agtaatgatg ttaacaataa
     1741 gaaaactaat tccaaagttg ttgaattttt aaaataaaag tttctcaaaa tagttaattg
     1801 ataagttaag ggatttaact gaccaatcca tctcacaata ctaggagcag aatctattaa
     1861 gtagaaaaca ggagctgtaa aaccaagcgg tgttagtaca aaacgtatta caatatcacg
     1921 tcttctgtaa tcattgataa acattgtaag taaaattgca agggagtccc aaaataataa
     1981 aacaacaata gataacaaaa ctgtataaaa aaatctatcc attgcgatat ttatctcaaa
     2041 cgctaacgta attatatata taataatttc
//
LOCUS       DAVLXF010000152         2038 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLXF010000152 DAVLXF010000000
VERSION     DAVLXF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.152"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            22..258
                     /locus_tag="VYO12_002070"
     CDS             22..258
                     /locus_tag="VYO12_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEV1588597.1"
                     /translation="MNNVVTTQISIQLSQELNSLLNAIVEENNTTKTDFIRQAVIEKI
                     EDMYDIKVADEAYKKWVECGKKATSHEEMMRRYG"
     gene            251..982
                     /locus_tag="VYO12_002071"
     CDS             251..982
                     /locus_tag="VYO12_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1588598.1"
                     /translation="MAKLYAISDIHGYLDEFRDALNKVNLNDKDNRLFLLGDYLDNGL
                     QSFQVISKIIELEEVYPNQIITLLGNHEEWFYDWLILDKPTASAFPETIKSFFSPEEL
                     NYIFKSNANNFETGVRNEIKNNIKFNPFINWFKKRYRDKRYYETDTQIFVHAGIDEEA
                     GMLWKELTSSKIFTNKFPITTGRFHKAIISGHIASWEVAKDRRYLGKIYYDSKSHYLI
                     DGDVTNSKTIPVLYYDTITQKYHFL"
     gene            complement(1093..1176)
                     /locus_tag="VYO12_002072"
     tRNA            complement(1093..1176)
                     /locus_tag="VYO12_002072"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1140..1142),aa:Leu,seq:caa)
     gene            complement(1186..1257)
                     /locus_tag="VYO12_002073"
     tRNA            complement(1186..1257)
                     /locus_tag="VYO12_002073"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1223..1225),aa:Gln,seq:ttg)
     gene            complement(1268..1340)
                     /locus_tag="VYO12_002074"
     tRNA            complement(1268..1340)
                     /locus_tag="VYO12_002074"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1305..1307),aa:His,seq:gtg)
     gene            complement(1355..1425)
                     /locus_tag="VYO12_002075"
     tRNA            complement(1355..1425)
                     /locus_tag="VYO12_002075"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1391..1393),aa:Trp,seq:cca)
     gene            complement(1430..1510)
                     /locus_tag="VYO12_002076"
     tRNA            complement(1430..1510)
                     /locus_tag="VYO12_002076"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1474..1476),aa:Tyr,seq:gta)
     gene            complement(1523..1595)
                     /locus_tag="VYO12_002077"
     tRNA            complement(1523..1595)
                     /locus_tag="VYO12_002077"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1560..1562),aa:Phe,seq:gaa)
     gene            complement(1599..1672)
                     /locus_tag="VYO12_002078"
     tRNA            complement(1599..1672)
                     /locus_tag="VYO12_002078"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1636..1638),aa:Met,seq:cat)
     gene            complement(1683..1772)
                     /locus_tag="VYO12_002079"
     tRNA            complement(1683..1772)
                     /locus_tag="VYO12_002079"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1734..1736),aa:Ser,seq:tga)
     gene            complement(1856..1929)
                     /locus_tag="VYO12_002080"
     tRNA            complement(1856..1929)
                     /locus_tag="VYO12_002080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1893..1895),aa:Ile,seq:gat)
     gene            complement(1964..2034)
                     /locus_tag="VYO12_002081"
     tRNA            complement(1964..2034)
                     /locus_tag="VYO12_002081"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2000..2002),aa:Gly,seq:tcc)
BASE COUNT          673 a          417 c          394 g          554 t
ORIGIN      
        1 taactattag gtggctgtta tatgaataat gttgttacta cacaaatttc tattcaactt
       61 agtcaagagt taaattcttt attaaatgct attgttgaag aaaataatac aacaaagact
      121 gactttatta gacaagctgt tatagagaaa attgaagata tgtatgatat taaggtcgct
      181 gatgaagctt ataaaaaatg ggttgaatgt ggcaaaaaag ctacctcaca tgaagaaatg
      241 atgagacgct atggctaaat tatacgcaat atctgatata catggatact tggatgaatt
      301 cagagacgct ctcaataaag taaatttaaa cgataaagat aatcgcctat ttttactagg
      361 agattatctt gataatggac ttcaatcttt tcaagtcatt tccaaaataa tcgaacttga
      421 ggaggtttat cctaatcaaa ttattactct tctagggaat catgaagaat ggttttatga
      481 ttggttgatt ttagataaac ctactgcttc agcatttcca gaaacaataa aaagcttctt
      541 ctctccagag gaattgaact atatttttaa atcgaatgct aacaactttg aaacaggtgt
      601 tagaaatgaa attaaaaata atatcaaatt caacccattt ataaactggt ttaaaaagcg
      661 atatagggat aaacgctatt atgaaactga tacccaaatt tttgttcatg caggcattga
      721 tgaagaagca ggaatgcttt ggaaagaact aacgagttca aagatattta caaacaaatt
      781 tccaattacc actggtagat ttcataaagc tattatttcg ggccatattg cttcttggga
      841 agtagctaaa gatagaagat acctaggtaa gatttactat gatagtaaaa gtcattactt
      901 aattgatgga gatgtaacga atagcaaaac aatacctgtt ctttactatg atacaattac
      961 ccaaaaatat cactttctat aacttttaat catgtgcttt tatatgaaat tagtaattat
     1021 tagtaacaat tccaagaaat ttcttatgac ataacaaaaa gacaaggtcc taaaaccttg
     1081 tctttaatac tataccggcg gccggggtcg aaccggcacg tccttgcgga cactggattt
     1141 tgagtccagc gcgtctgcca attccgccac gccggcaaat agtaactggg gtagctggat
     1201 tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta taccccaata
     1261 taaaataggc gagtgatggg gatcgaaccc acgcatgcca gagccacaat ctggtgtgtt
     1321 aaccacttca ccacacccgc cataattcta ttaacacggg cagtaggaat tgaacccaca
     1381 ctgaaggttt tggagacctt agttctacct ttaaactatg cccgtaaaat ggaaggggag
     1441 ggattcgaac ccccgaaccc gaaggagcgg atttacagtc cgccgcgttt agcctcttcg
     1501 ctacccttcc aaaatatata aatggcgcga gacggaatcg aaccgccgac acatggagct
     1561 tcaatccatt gctctaccaa ctgagctacc gagccttatt gcgggagcag gatttgaacc
     1621 tacgaccttc gggttatgag cccgacgagc tacctagctg ctccatcccg cgttaatata
     1681 aaaggaggat gtgggattcg aacccacgca cgcttttaca cgcctgacgg ttttcaagac
     1741 cgttcccttc agccggactt gggtaatcct ccaaaatagt tttatacggg aacaagcccg
     1801 tgtcctctta gtgaacagat aatattccaa tcccctagta atggactaaa cactatgggc
     1861 acgagtggac tcgaaccacc gacctcacgc ttatcaggcg tgcgctctaa ccacctgagc
     1921 tacgcgccca agtcaaaaac ttggtacaaa gaacaaagtt caaagcgggt gacgagaatc
     1981 gaactcgcga caacagcttg gaaggctgta gttttaccac taaactacac ccgctaaa
//
LOCUS       DAVLXF010000153         2028 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLXF010000153 DAVLXF010000000
VERSION     DAVLXF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.153"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            140..907
                     /locus_tag="VYO12_002082"
     CDS             140..907
                     /locus_tag="VYO12_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEV1588599.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            909..1541
                     /locus_tag="VYO12_002083"
     CDS             909..1541
                     /locus_tag="VYO12_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588600.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
BASE COUNT          623 a          359 c          402 g          644 t
ORIGIN      
        1 catataattc attacaagtc agtttagttg gagtattact actttttata agtatggtaa
       61 atattttatg ggctaaaaaa agtaaatgaa aataaaattt ggagccctct gaaaaagtaa
      121 gtcctacagt tcaactaaaa tgagtcaaaa gatgaatcac cttgatgtag gggagtttgt
      181 cttattgctg cctgaacacc tccgttcaga ggaagaacat tataaatctg tttttgaaga
      241 cgacttaacc agtcgcatat ctagtcaaga tgaacgacag caaatgactg ctacggtagg
      301 ttatttagaa tcaggtcagg atcgttttgt gtataatacg acccctattt cttaccagca
      361 gtttttgaaa gatccaatca tcattgttat aacaccccaa tcaactggtc cacagtccat
      421 tttgttttgg atagacgcag tacagaacta cgttctcttt aatcaattgt ctgatgccca
      481 ggagcttatc cagagacaag gcattgaaaa ttgggtctca gaaatgcaaa caggttacca
      541 caactacatc acattattgg ataatatcca gagggaacgt tgggtaatgc tagcaggagc
      601 tgtgcttggg attgcaactt caatcttgtt gtttaacact atgaataggc tctactttga
      661 agaatttaga cgtgccattt ttatcaaacg cattgcaggt ctcaggttct tagaaatcca
      721 tcgcacttat ctctttgctc aactgggtgt gtttttactg ggatttgttg cgagtgtatt
      781 tcttcaggta gagataggag ttgctttctt agtcttgtta ctctttactg gtctatctct
      841 tttacagtta catgtccaaa tgcagaaaga aaacaagatg tccattcttg ttttgaaggg
      901 aggttaatat gattgaactt aaacaggtga gtaaatcttt tggagaacga gagttatttt
      961 cgaatctttc aatgacattt gaggctggaa aagtctatgc cttaattggt tcaagtggta
     1021 gcggaaaaac aaccttgatg aacatgattg ggaaattaga accttatgat gggacgattt
     1081 tttaccgagg taaagacttg gccaattata aatcgagtga ttttttccgt cacgaattgg
     1141 gctacctctt ccagaacttt ggcttaattg aaaaccaaag tattgaagaa aaccttaagc
     1201 taggtctcat tggtcaaaag ttgagtcggt cggaacagcg gttgaggcag aagcaggctt
     1261 tagaacaggt cggcctggct tatcttgacc tagataagcg catctttgag ttatcgggcg
     1321 gagaatcgca acgggttgcc ttggcaaaaa ttatcttaaa gaatccaccc tttattctgg
     1381 cagatgagcc aacagcttca atagacccag caacctctca gttgattatg gagattttgc
     1441 tatctcttcg agatgataat aggctaatca ttatcgcaac acataatccg gcaatttggg
     1501 agatggctga tgaagtgttc acgatggatc atctgaaata aaaatccttg tttttaattg
     1561 cacgatgagt tactgaaata ttatcatgaa tcaagaattg gagttaattt agaattgtac
     1621 tttattaata ttgaggtaac tttttcttga taaaggaaga aataatggag aggaagttag
     1681 aatgaaaaaa ttcgacaatt atattattga gaagccttgc gattctaatt cagataaact
     1741 gcaaaaaatc ttaataattg aaagtttggt agatgatatt ttgcaatttt ctctcagaat
     1801 caataatagt gtaggagaga ttttcctcct acaaccgttt taaaagaaaa ctatctttat
     1861 cccaaatcat tcatacctct ctcaattaga tgtaacttac aaaacccctg acctcatgag
     1921 ccactttctt cctcctcatg aggtcagttt tactttctgc tgttccagta tcgtttttcc
     1981 tcgctagatt tcctcaaaag ggcagactcc tcccttggtg cgtcacac
//
LOCUS       DAVLXF010000154         1946 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLXF010000154 DAVLXF010000000
VERSION     DAVLXF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.154"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..459
                     /locus_tag="VYO12_002084"
     CDS             <1..459
                     /locus_tag="VYO12_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEV1588601.1"
                     /translation="VILSENSKKFISKELISYFCDKVYDEIFVEEVVPHVFLPYESDL
                     LLILPATANVIGKIANGIADDLVTATVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILK
                     ELGHIFLFESKKTYEVGLRKAIDSTCSMLQPQSLVKELIKLENIVLEEGH"
     gene            680..1786
                     /locus_tag="VYO12_002085"
     CDS             680..1786
                     /locus_tag="VYO12_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV1588602.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGSA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            1762..>1946
                     /locus_tag="VYO12_002086"
     CDS             1762..>1946
                     /locus_tag="VYO12_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588603.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDL"
BASE COUNT          715 a          231 c          334 g          666 t
ORIGIN      
        1 gtgatacttt ctgaaaattc taaaaaattt ataagtaagg aattaattag ttatttttgt
       61 gataaagttt atgatgaaat atttgttgaa gaggtagttc cgcacgtttt tctgccatat
      121 gaatctgact tacttcttat tttaccagct acggcaaatg tgattggcaa aattgctaat
      181 ggtattgctg atgatttagt tacagcaact gttttaaact ttaataaaaa aataattttt
      241 tgtcccaata tgaactctac tatgtgggac aatcacatag ttcaaagaaa tgtatcaatt
      301 ctaaaggagt tgggacatat atttttattt gagtctaaaa aaacatatga ggtaggattg
      361 cgtaaagcaa tagattcaac atgttcaatg ttacaaccac agtcgttagt aaaagaactt
      421 atcaaattag aaaatattgt ccttgaagag ggacattaaa aactactgag aatattaagg
      481 aggggaaaaa atggaaaatt catcaatcga tgtagatatg ctgttggaag aattgacaca
      541 agaagcaatg gtcgttgttg ctgttgataa ggactgttaa tttaaactta tggcaatata
      601 tgaaaggtta ctggatgttt taaattatgc aggcagtagc cttttattat atacaaatgg
      661 ataaagtaag gataatacaa tgattaataa aaaaatacaa caagttgttt tggaatcatt
      721 acagaatttt ttgaatggga acttcatttc gccttgtgta gtctatgatt ttggcttgct
      781 ggaaactgta cttgatgaat ttaaaaatca aattcctgta acattcaatt accaactttt
      841 ttatgccgtt aaagcaaatt caaatgagaa gatacttgaa ttcttagtag ataaaattga
      901 tggagttgat gtggcgtcat tatctgaatt agatgtggct aaaaaatttt tcccaccaac
      961 tcaaatttct gttaatggtt ccgcattttc ttatgaaact ttatataatc tgattaaaaa
     1021 acaatataaa gttgatatta actttttgga acatcttcaa caattttccc caaaagaatc
     1081 tgttggaata agagtaacgg agccagatga acttaataat cgtatgagtc gatttggaat
     1141 aaatatttgc agtgataatt ggactagtaa tttacaaaat cctttaatta cacgactgca
     1201 ttttcatttt ggagaaaaag atgataaatt tattgttaag ttagataaaa tattatttaa
     1261 gttacaagaa attaataaac ttagagaggt tagagaaata aatcttggag gcggttttat
     1321 gaaattattt atggaaaatc gtttgaaaga attttttcta tcacttatgg aaatctataa
     1381 aaagtacgat attgatagta ctgtgactac aataatagaa ccaggtagtg caattacttc
     1441 attttctgcc tatatgatta ctagcccagt taatgttagt gaggtgaatg agcagcaggt
     1501 tatcacgtta gacacatcaa tatacaccaa tacattatgg tttgttccgc atattattac
     1561 aacgttaaat tcaagtagta aagagcgtta tagtactatt ctctatggta atacctgtta
     1621 tgaacatgac aagtataaaa tgaaagtttc gcttccaagg ttaactcaaa atagcagtat
     1681 agtgtttttt cctgtaggag cttatataaa aagcaatcat tcaaatttac atcgtaatga
     1741 ttttatgcgg gaggtatatt tgtggacaaa aaacttgaca tattagataa agttaaggaa
     1801 tatttaggaa ataaaactac tcaaattctg gataatcaat ataaagaatt tttgaaactt
     1861 aatgatataa ggcgagcgtt tggtatttca gaaaaagtat taaacaattc ttttaatttt
     1921 acgagtaaag aatttaatga tttaat
//
LOCUS       DAVLXF010000155         1822 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLXF010000155 DAVLXF010000000
VERSION     DAVLXF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.155"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            213..1155
                     /locus_tag="VYO12_002087"
                     /pseudo
     CDS             213..1155
                     /locus_tag="VYO12_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285140.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1152..>1822
                     /locus_tag="VYO12_002088"
     CDS             1152..>1822
                     /locus_tag="VYO12_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1588604.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIV"
BASE COUNT          615 a          257 c          338 g          612 t
ORIGIN      
        1 ggttagggat tgcaacattt ttatttcctt tgggcctata ttcctatttt cgatggataa
       61 aacagtatga gagggataga ggactattcc taagtttact actatctctt ttatatgaga
      121 gcactcataa ctttctgtcc gtaactttct cctctataac aggagataat tttgttttac
      181 aatatcattt cccattcttt ttcgttgtga cggtgttaac ctattttgtt acattaaaaa
      241 tcatttacta tttccatttg gaactagcct attttgacaa ggactacctt tatcctttct
      301 tgaaaaaagt attttttgct ttactattgc tacatattgt atctttcgtt tcagatatgg
      361 taagtacgat taaacatttg aatagttttg gaagtatttt gtcatctatt gtctttatct
      421 ctctcctttt gaccttcttt gcaatgaatt ctcataaagt tcaaatggag aaagagattg
      481 ctttgaagca gaagaaattt gaacagaaac atttacagaa ttacacagat gaaattgttg
      541 gtctgtataa tgaaatccgt ggttttcgac atgattatgc tggaatgctt gtcagcatgc
      601 agatggcaat tgacagtggt aatttacagg aaattgacag aatttacaat gaagttttag
      661 tcaaagcaaa tcataaattg cgttcagata agtacactta tctttgattt gaacaacata
      721 gaagactcag ctttacgaag tttggttgct cagtcaattg tctatgctcg aaataatggt
      781 gtagagttta cactggaagt aaaagatacg attaccaagc ttccaattga actattggat
      841 ttggttcgta tcatgagcgt tttattgaat aatgctgtcg aaggatcggc tgatagctat
      901 aaaaagcaga tggaagtagc agttattaag atggaaactg aaacagttat tgtgattcag
      961 aattcatgta aaatgacgat gactccttca ggagatctat tttccttagg attctccact
     1021 aagggaagaa atcgcggagt cggattaaat aatgtgaaag aactactaga taagtacaac
     1081 aatattattt tagaaacaga gatggaaggc agtacattta gacaaatcat tagatttaag
     1141 agggaatttg aatgaaagtt ttaattttag aagatgttat tgaacatcaa gtgagactag
     1201 agagaatatt ggatgaaatt tcgaaagaat cgaatattcc aatatcatac aagacaacgg
     1261 gaaaagtccg tgaatttgaa gaatacattg aaaatgatga agtaaatcag ctttatttcc
     1321 tagatatcga tattcatgga attgagaaaa agggatttga agtggctcag ctcattcgtc
     1381 attacaatcc ttacgctatt atcgtcttta tcactagtcg atcagagttt gcgactctaa
     1441 cctataaata ccaggtatca gccctagatt ttgttgataa ggatatcaat gatgagatgt
     1501 ttaagaagag aattgagcaa aatatcttct acacgaagag tatgttactt gaaaatgaag
     1561 atgttgtaga ttatttcgac tacaattaca agggaaatga tttaaaaatt ccttaccatg
     1621 atattttgta tattgaaaca acaggggtat ctcataaatt gcgcattatt ggtaagaatt
     1681 ttgcaaaaga gttttatggt accatgacag atattcagga aaaggacaaa catactcagc
     1741 gattttattc tcctcacaag tcatttttgg taaatatagg caatatcaga gaaattgatc
     1801 gaaaaaactt agaaattgtt tt
//
LOCUS       DAVLXF010000156         1711 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLXF010000156 DAVLXF010000000
VERSION     DAVLXF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.156"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            252..1613
                     /gene="dnaA"
                     /locus_tag="VYO12_002089"
     CDS             252..1613
                     /gene="dnaA"
                     /locus_tag="VYO12_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1588605.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT          589 a          294 c          299 g          529 t
ORIGIN      
        1 caagaagaat atagtagaat tatcaacagc ctgaaataag gctgttcttt tattttttta
       61 tctcacaagg ttatccacta tgtttttcga taaaaagctt aataaatcaa taatttcttc
      121 ttttatcccc aacctgtgga taaagtttag taacattgtg gattattttt cacagcttgt
      181 ggaaaattct tgctatctat ggtaaaatat ctctagtatt aaacttttaa atagtaaagg
      241 aggagaaagg attgaaagaa aaacaatttt ggaatcgtat attagaattt gcacaagaaa
      301 gactgactcg atccatgtat gatttctatg ctattcaagc tgaactcatc aaggtagagg
      361 aaaatgttgc cactatattt ctacctcgct ctgaaatgga aatggtctgg gaaaaacaac
      421 taaaagatat tattgtagta gctggttttg aaatttatga cgctgaaata actccccact
      481 atattttcac caaacctcaa gatacgacta gctcacaagt tgaagaagct acaaatttaa
      541 ctctttatga ctatagtcca aagttagtat ctattcctta ttcagatacg ggattaaaag
      601 aaaagtatac ctttgataac tttattcaag gggatggaaa tgtttgggct gtatcagccg
      661 ctttagctgt ctctgaagat ttggctctga cctataaccc tctttttatc tatggaggac
      721 caggccttgg taagactcac ttattaaacg ctattggaaa tgaaattcta aaaaatattc
      781 ctaatgcgcg tgttaaatat atccctgccg aaagctttat taatgacttt cttgatcacc
      841 taagacttgg ggaaatggaa aagtttaaaa agacctatcg tagtcttgat cttttgttaa
      901 tcgatgatat ccagtcactc agcggaaaaa aagtcgcaac tcaggaagaa tttttcaata
      961 cctttaacgc ccttcatgac aagcaaaaac agattgtcct aacgagtgat cgtagtccaa
     1021 aacatctaga agggctcgag gagaggcttg tcacgcgttt tagttgggga ttgacacaaa
     1081 ctatcacacc ccctgacttt gaaacacgta ttgccatttt acaaagtaaa acggaacatt
     1141 taggctacaa tttccaaagt gatactctag aatacctagc tgggcaattt gattcaaatg
     1201 ttcgagatct tgagggagcc atcaacgaca tcactttaat tgccagagta aaaaaaatca
     1261 aggatatcac tattgatatt gctgcagaag ccattagagc ccgcaaacaa gatgttagcc
     1321 aaatgctcgt catcccaatt gataaaatcc aaactgaagt tggtaacttt tatggtgtta
     1381 gtatcaaaga aatgaaggga agtagacgcc ttcaaaatat tgttttggcc cgtcaagtag
     1441 ccatgtattt atctagagaa ctaacagata atagtcttcc aaaaattggg aaggaatttg
     1501 ggggaaaaga tcataccaca gtcattcatg cccatgccaa aataaaatct ttgattgatc
     1561 aagacgataa tttacgttta gaaattgaat caatcaaaaa gaaaatcaaa taatttgtgg
     1621 ataactttta gttttttatc ttttttatcc acatttttta aacaagctaa aaaacttgat
     1681 atgacttgtt taaaggctgt tttccacaga t
//
LOCUS       DAVLXF010000157         1706 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLXF010000157 DAVLXF010000000
VERSION     DAVLXF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.157"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            31..300
                     /gene="blpJ"
                     /locus_tag="VYO12_002090"
     CDS             31..300
                     /gene="blpJ"
                     /locus_tag="VYO12_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV1588606.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGSGLTCLGGMVGDKLGLSW"
     gene            369..503
                     /locus_tag="VYO12_002091"
     CDS             369..503
                     /locus_tag="VYO12_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEV1588607.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWEILPKQVLEEQQQEVFN
                     "
     gene            440..598
                     /locus_tag="VYO12_002092"
     CDS             440..598
                     /locus_tag="VYO12_002092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588608.1"
                     /translation="MQLGDFAKAGVGGAAARGLQLGIKTGTWQGAATGAAGGAILGGV
                     AYAATCWW"
     gene            601..720
                     /locus_tag="VYO12_002093"
     CDS             601..720
                     /locus_tag="VYO12_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1588609.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDNLIRKIFSKFPKKDTDSTP"
     gene            1017..1181
                     /locus_tag="VYO12_002094"
     CDS             1017..1181
                     /locus_tag="VYO12_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588610.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            1178..1582
                     /locus_tag="VYO12_002095"
     CDS             1178..1582
                     /locus_tag="VYO12_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1588611.1"
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
BASE COUNT          524 a          234 c          366 g          582 t
ORIGIN      
        1 caaattgaaa ataaaaagga ggtattcatc atgaatacaa aaatgttgtc acagttagag
       61 gttatggata ctgaaatgct tgcgaaagtt gaaggggggt atagctccac tgattgtcaa
      121 aatgcactga ttacaggagt cactacggga attataactg gtggaactgg agcaggtttg
      181 gccactttag gagtagctgg acttgctggt gcatttgttg gagcacacat tggtgcaatt
      241 gggagtggat taacatgctt aggaggtatg gttggtgata aattgggact aagttggtaa
      301 gacttatagt aaaattatga ggattctatt ctgatttatt ttagtttttt aaaaaaggag
      361 tatttgtcat ggatacaaaa atgatgtcac aattttctgt tatggatact gaaatgcttg
      421 cttgcgttga aggtggcgga tgcaattggg agattttgcc aaagcaggtg ttggaggagc
      481 agcagcaaga ggtcttcaac taggcattaa aacaggaaca tggcaaggtg cagcaactgg
      541 tgctgcgggt ggagctatac ttggaggtgt agcctatgca gcaacatgtt ggtggtaatt
      601 atggatttta aaagttttat tattggttta gtagttggta tatttggtcc ttatatggat
      661 aatttaatta gaaaaatatt ttcaaaattt cccaaaaagg atacagatag taccccctaa
      721 aaaataaatt tctcccctgt atttgctagc ttaatttaca ggggagtttc tttaattttg
      781 tttcgcctgt caaaatgata tgtgatagca ggatgaaaag tctacaaaca agttggtatc
      841 aatacaatat aaagaggaaa gtatgttttt catgcttgct tttttaattt ttacaattca
      901 agaggttttg atgaccattt atgatttgag tgatccaagg tcaaaataag tgctatacta
      961 gcagtgtaaa tgttcttgct caactaagat aaatcgcaca tttaggagga aatcttatga
     1021 agaaaaaaat actgattatt ttcgttctgt atctaatcat gtccatcttt ctatatccgc
     1081 ttagggagag tgcttggtat cagctatttt ataccatagc ctatgtgatt gcagttatga
     1141 tctattttgc tatcaataaa aagaaaggag aaaagaaatg aaaacttttc ttgctaaaaa
     1201 acggaacatc ttccttgcga gattgttctt aggtcagttg cccttgcttg tttctactta
     1261 tctatttcta tctcgtcagt ttttaaattt ttccgtagtt ttccaatttc ttttagtagt
     1321 tattaacttg gcttctattt tggttactgt ttatctcact agggaaatga ggataagaga
     1381 gtttgaagat gatgatttgg ttagtcctag aaccaatcaa cttatgttca tcggcttgac
     1441 aggttttatg tctattattt gtttgtatag aggtatcaca gcaggagaat cctaccaaca
     1501 aataatcgct tatattggcg ctgttctctg cttaatcatc atgcttctgc tcatgtgggg
     1561 cttgaagtat tataaaaagt agagcttaat gaagtataaa agaaaagggt aagtgttttt
     1621 gcaatactta cccttttata atagattccc tgcgaaacaa aatatggtat agtatagtag
     1681 attgaaacta gaatagtacg cctcta
//
LOCUS       DAVLXF010000158         1705 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLXF010000158 DAVLXF010000000
VERSION     DAVLXF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.158"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            31..834
                     /locus_tag="VYO12_002096"
     CDS             31..834
                     /locus_tag="VYO12_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1588612.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            836..1645
                     /locus_tag="VYO12_002097"
     CDS             836..1645
                     /locus_tag="VYO12_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1588613.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT          587 a          299 c          295 g          524 t
ORIGIN      
        1 aataaattta aaaaatctta ggagtttaaa atgaaacaac taaccgttga agatgccaaa
       61 caaattgaat tagaaatttt gaattatatt gatactctct gtaaaaagca caatatcaac
      121 tatattatta actacggtac tctgattggg gcggttcgac atgagggctt tatcccttgg
      181 gacgacgata ttgatttatc catgcctaga gaagactacc aacgatttat taacattttt
      241 caaaaggaaa aaagcaagta taagctccta tccttagaaa ctgataagaa ctactttaac
      301 aactttatca agataaccga cagtacaact aaaattattg atactcgaaa tacaaaaacc
      361 tatgagtctg gtatctttat cgatattttc cctatagatc gctttgatga tcctaaggtc
      421 attgatactt gttataaact ggaaagcttc aaactgctgt ctttcagtaa acataaaaat
      481 attgtctata aggatagcct tttaaaagat tggatacgaa cagccttttg gttgctcctt
      541 cgaccggttt ctcctcgtta ttttgcaaat aaaatcgaga aagaaattca aaaatatagt
      601 cgtgaaaatg ggcagtatat ggcttttatt ccttcaaaat ttaaggaaaa ggaagtcttc
      661 ccaagtggta cctttgataa aacaatcgat ttaccctttg agaatttaag ccttcctgca
      721 cctgaaaaat ttgatactat tttgacacaa ttttatggag attatatgac cctaccacca
      781 gaagaaaaac gcttctacag tcatgaattt cacgcttata aattggagga ttaggatgca
      841 atatttagaa aaaaaagaaa ttaaagaaat tcaactagcc ctgctggact atattgatga
      901 gacttgtaag aaacatgata ttccttattt tctcagttat ggaaccatgc ttggagccat
      961 ccgccacaaa ggtatgattc cttgggatga tgatattgat atttcccttt atcgtgagga
     1021 ttatgagcgt ttactgaaga ttattgaaga agaaaatcac cctcgctaca aggttctttc
     1081 ctacgataca tcttcttggt acttccataa tttcgcatcg attttggaca cttctactgt
     1141 tatagaagac catgttaagt acaagcgtca tgataccagc cttttcatcg atgtcttccc
     1201 aattgatcga tttacagact tgagcattgt cgacaagagc tataagtatg tggctcttcg
     1261 tcaactagct tatatcaaaa aatcacgagc agttcacggt gatagcaaac taaaagattt
     1321 tcttagatta tgtagctggt acgctctccg atttgtcaat cctcgctact tttacaagaa
     1381 aattgatcaa ctagtcaaaa atgctgtaac caacactcct caatatgaag gaggagttgg
     1441 gatcggtaag gaagggatga aagaaatctt cccagttgat acctttaaag aactgatttt
     1501 aactgagttt gagggccgta tgttgcctgt tcccaaaaaa tatgaccaat ttttaaccca
     1561 gatgtatggc gattatatga caccaccatc aaaagaaatg caagagtggt atagtcatag
     1621 cattaaagct tatcgcaaaa actgattgag gggtattata caaactacta agatagaggt
     1681 tattcaaaaa cataatttta gtaga
//
LOCUS       DAVLXF010000159         1648 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLXF010000159 DAVLXF010000000
VERSION     DAVLXF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.159"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..101)
                     /locus_tag="VYO12_002098"
     CDS             complement(<1..101)
                     /locus_tag="VYO12_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1588614.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKK"
     gene            complement(205..354)
                     /gene="blpO"
                     /locus_tag="VYO12_002099"
     CDS             complement(205..354)
                     /gene="blpO"
                     /locus_tag="VYO12_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1588615.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(598..801)
                     /gene="blpN"
                     /locus_tag="VYO12_002100"
     CDS             complement(598..801)
                     /gene="blpN"
                     /locus_tag="VYO12_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV1588616.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(817..1071)
                     /gene="blpM"
                     /locus_tag="VYO12_002101"
     CDS             complement(817..1071)
                     /gene="blpM"
                     /locus_tag="VYO12_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV1588617.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            complement(1221..>1648)
                     /locus_tag="VYO12_002102"
     CDS             complement(1221..>1648)
                     /locus_tag="VYO12_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1588618.1"
                     /translation="LANYSGKKKFHAMKTQAIVTSQGRIVSLDITVNYCHDMKLFKMS
                     RRNIGQAGKILADSGYQGLMKIYPQAQTSRKSSKLKPLTVEDKACNHALSKERSKVEN
                     IFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
BASE COUNT          523 a          375 c          216 g          534 t
ORIGIN      
        1 tcctttttgg aagattttga aaatattttt ctaattaagt catccatata aggaccaaat
       61 ataccaacta ctaaaccaat aataaaactt ttaaaatcca taattaccac caacatgtcg
      121 ctgcataggc cacacctcca agtatagctc ctcccgcagc accagttaca cctattccta
      181 tagcaaatgg tcccaataga aatgtcaaac cgttgttgca cacccatcaa ttgcgccata
      241 tgcaacccct gctgcacaac taatttttct tccccaatca atatctccac cttcaacgca
      301 agcaagcatt tcattatcca taactgcaaa ttgtgacatc atttttgtat ccatatagta
      361 tatcactttt cagttacgga acaagtttaa tataaaaatt atcaaaaaaa cataggcaat
      421 aaagagaaaa attaatttat catagattag aaataatatg acaaaacaat tcaatgatgt
      481 taattcaata gtcttttgtt ttttatcgga gatacttatg gatagataaa taagataggt
      541 ttgaaaagcg aagagaataa taaagaatat agccttcata aaatttagct ttcattttta
      601 tgatgtagcg gtataggcta aatatccaca aaccactgct cctccaattc ctcctattgc
      661 agcgccccat ggtcctagaa gtctcccata tttcactcca cccgctgcac aacctaaagc
      721 agcaactaca gctgctcctc cggaattacc tccataaacc tcactcagca ttgtttcatt
      781 tatattacaa taagtattca tacaagtctc cttttattaa aatccacccg ttgcccctgt
      841 tactcctgcc caaagatcca caccaaattt agctcctatg tatccacatg ctcccataaa
      901 tggtgctcca acaccactcg cagcacaaat agctgtccct agcccccagc caccaaaagc
      961 agcaccacca ccttctaaga cattagtttg ccaattattc ttgcctcctt caatactaga
     1021 taacatagtt atatccattt catgaaattg ttccataatt tttgtatcca tgacaaatac
     1081 tcttttttat ttttaatttt tgtcttgttg taactttgac aagtttagta tatcatcgtt
     1141 ttttaaaatt tttcatccag atcttgaata gtcatcgaaa cgtcttgaat tgcaaaaatt
     1201 acattagact tcctgcaaaa ctagaatcct agttcatgat tgataatacc agcaatcaaa
     1261 ttcattcgta atccgaagcg tttacgatga tttcgatagg ttgttgaaaa cattttaaac
     1321 gtttttactt tggcaaagat gttctcaacc ttgcttctct ccttagatag cgcatggtta
     1381 caggctttat cttcaactgt tagcggtttg agtttgctgg atttacgtga agtttgtgct
     1441 tgaggatata tcttcatgag cccttgataa ccactgtcag ccaagatttt accagcttgt
     1501 ccgatatttc tgcgactcat tttgaacaac ttcatatcat gacaatagtt cacagtgata
     1561 tccaaagaaa caattctccc ttgacttgtg acaatcgctt gagtcttcat agcgtgaaat
     1621 ttctttttac cagaataatt cgctaatt
//
LOCUS       DAVLXF010000160         1630 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLXF010000160 DAVLXF010000000
VERSION     DAVLXF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.160"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            77..484
                     /locus_tag="VYO12_002103"
     CDS             77..484
                     /locus_tag="VYO12_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1588619.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQTDEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            649..948
                     /locus_tag="VYO12_002104"
     CDS             649..948
                     /locus_tag="VYO12_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1588620.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1209..1532
                     /gene="tnpB"
                     /locus_tag="VYO12_002105"
     CDS             1209..1532
                     /gene="tnpB"
                     /locus_tag="VYO12_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1588621.1"
                     /translation="MIIQLSDLGQVHLVCSKIDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDDQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          551 a          325 c          288 g          466 t
ORIGIN      
        1 taataacgat actagataat gaggaaaatg caatggcaaa cccaactctc ggagaaaaaa
       61 agcgaataca gactatattg ctcgctatgg cgtatatgca gttatcccta atcctgaaca
      121 aaaacaaatt gttcttgttc aagaaccaaa tggtgcttgg ttcctaccat gtggagaaat
      181 tgaagcaggt gaaaatcatc aggaagccct aaagcgtgag ttgattgaag agcttggttt
      241 cacagcagaa attggtacct attacggaca aactgacgaa tatttctatt ctcgtcatcg
      301 tgatacctac tactacaatc ctgcctacct ctatgaagca acttctttca aagaagtaca
      361 aaagccacta gaaaacttta atcatattgc ctggttccct attgacgagg ctatccaaaa
      421 ccttaaacgt ggtagccata aatgggccat tgaatcttgg aaaaaacagc ataagattgg
      481 ctaatactct tcgaaaataa aattcaaacc acgtcagcgt cgccttaccg tactcaagta
      541 tagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa atcaagcgac
      601 tttattcaaa aagtgctata atagaattag aaaatcggag gaaatattat gaagcttatc
      661 aatacgacaa actcacactc gcaacttgta aaaagtcaac tagaaagtac cgatgcaacc
      721 cttgttgagg tttattctgc agggaataca gatgttattt ttacccaagc tccgcttcac
      781 tatgaaatcc tcatctcaaa caaacaccgt gctattcgtg agcctgaaat cgaaaccatt
      841 caagaatttt tcttgaaacg taaaattgat aaggcctctg ttgatgaagc caatatcaag
      901 acactctact cagagaaatt aattggaatt tcaatcccaa tcaagtaaga aaattgagta
      961 aaaactcgat ttgagagaaa gtccaataag tctttccata acaaaacgca tagtaacaag
     1021 agtttctaca cccactatta tacgttttta tcattttgaa atctgctgct aatcaatgaa
     1081 gaagcaaaga tcaaactaag cagctagtta caagttacgt aagcgcgtca taacaaggta
     1141 tctatcctaa ttccccaact tccagtatac tagataaaaa ataattatcg gaggaaaggt
     1201 attgtactat gataattcaa ctaagtgatt taggtcaagt tcaccttgtt tgtagcaaga
     1261 tagatatgag gcagggaata gactcattag cctatgtagt taaaacccac tttgaattgg
     1321 atcctttctc cggtcaagtc tttctctttt gtggtggacg taaagaccgc tttaaagccc
     1381 tttactggga tgatcaagga ttttggctac tatataaacg ctttgagaac ggcagactga
     1441 cttggcccag tacagaaaag gatgtcaaag ctctcacacc tgaacaagta gactggctta
     1501 tgaagggctt ttctatcact ccccaaatat agtggattta aactagaata gtacacctct
     1561 gcttctaaaa cattgttaga aatcgatttg actgtcctga tcgatttgtc ctgttcttgt
     1621 ttcattttac
//
LOCUS       DAVLXF010000161         1580 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLXF010000161 DAVLXF010000000
VERSION     DAVLXF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.161"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..242
                     /locus_tag="VYO12_002106"
     CDS             <1..242
                     /locus_tag="VYO12_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588622.1"
                     /translation="IYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVIN
                     EFIACLNSRCMPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            557..1534
                     /locus_tag="VYO12_002107"
     CDS             557..1534
                     /locus_tag="VYO12_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588623.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINNPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYIGIKHVSIRR"
BASE COUNT          540 a          210 c          260 g          570 t
ORIGIN      
        1 tcatttacga taatgaagca ttaagagatt tgtcaaaaca atatttaaac aaaaatattt
       61 cggaattaca agatggttct ttcgagggcg gtatacaaga tgctgttgcc tctaaacaaa
      121 tagtagttat aaatgaattt atagcttgtt taaattccag atgcatgcca tcccaatatg
      181 atagattttt ttataaagat agaccttata ttttttccag aaagagtcct attaaggggt
      241 gaaaatttgt tacatttgga ttaacaagtg tcctagggat tagaaataac tctagtatag
      301 ctagatattt ttgagtaact agaaatatca ttagtctacc catatgattg ccagaaaaga
      361 tggctgatga atcttattta tactgttgca gtaggcgttg tctaggataa ataagctatc
      421 ttgatggggg agattggaat aattttagta gaaacattaa aggattatgg acaagtagcg
      481 taggattagg tgatgcaatg tgctaagttg gcttggtgtt aacagtagta ttatttaaca
      541 atggaaagta gactaaatga aaaataagcg ttattttttt gacacaattt taataatttt
      601 actgcttata agcacgatat tttgtgtcag cccagtattt ataaaattgg acatcttagg
      661 aactccctct catgccatct taacctttgt attggcgata ccgttattct atattttgtc
      721 tcaatgctta catactttgc tattactagt ttcctcaatc ttttgtaaat taagaccaat
      781 ttatttttat tttatatttg tgatcattat aggagctagg aaatactatc gaattctctt
      841 tcaccaacta atgggattta gtccaggaat agcagtgttt tataaggaga gtcaaacaac
      901 taagaatctg tttaaatttt actacttctt gtactttaca acactaattt catactattt
      961 tttctttact tttgtgtatg acaaaccgtt gcttttgcct cttatttcat tttctattat
     1021 catagcatta gtacaaaaac tttatagaat tgagaatcaa caattatttc tgttaaaatc
     1081 aaaggtacta acaatactgg aatctaagaa gaattgtgag tttaatcttc aggattatca
     1141 tgaaatttgg aaattgcaat caaaatctga gttgccatgc gtggctttat cttatatcag
     1201 tttaatcaag ccatatcttt cagagagtgt gcgtgaacaa attgatttat tagaagttaa
     1261 gcgatttaaa aaaataaata atccaatatc actttatggt atgttggatg ttataaaatt
     1321 aaatctatat ttacggcatt ataatgaaaa aaataaatat gaatcaatgt taaagaaaat
     1381 tttagaggta agaccagact ttgttttaat cgagcagaat attgatgata gtttaaatag
     1441 ctcgcaacca ttatctttat ctttagccat ttcagaaatc caacttttgc ttgaagtgta
     1501 tatagggatc aaacatgttt caattaggag ataaaactag aataatattt gccattatag
     1561 gggcaagttg catattagtc
//
LOCUS       DAVLXF010000162         1565 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLXF010000162 DAVLXF010000000
VERSION     DAVLXF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.162"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            37..723
                     /locus_tag="VYO12_002108"
     CDS             37..723
                     /locus_tag="VYO12_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1588624.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            724..>1565
                     /locus_tag="VYO12_002109"
     CDS             724..>1565
                     /locus_tag="VYO12_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1588625.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAA"
BASE COUNT          507 a          219 c          308 g          531 t
ORIGIN      
        1 attagtaaaa aaaataaaaa aacggaggtt ttctgaatgg aaactaaaaa aattaaaaat
       61 ttaaaaggtc aaataattgt ttcctgccaa gcgttagaag gagagccact ttatactcca
      121 aatggaggag tgatgccgtt attagcaaaa gccgcctttc aagcaggcgc taaaggtatt
      181 cgtgctaatt ctgtccgcga tatttcagaa ataaaagaag aagttgactt accaattata
      241 ggtatcataa aaagagatta cgatggcttt gaacctttta tttcagcaac tatgaaagag
      301 attgatgaat tagttagtga aggagtagac attttagctt tggattgtac taatcgttca
      361 agaccaggtt atgataatat tactgacttt attcatgata ttaaagtcaa gtatccaaac
      421 caattattaa tggctgatat ttctactttt gaagaaggga aagtagctgc tgaatcggga
      481 gtagattttg tgggtacgac attatctggt tatacaccat atagccctaa aaaagataat
      541 cctgattttg agttagttga aagattggta aaagagcttg acgtaccagt tattgctgaa
      601 ggaagaataa gtactccgga acaagctaga aaaatgttag atttaggtgc ttacgcagtg
      661 gttgttggag gagctatcac tcgtcctttg gaaatagcga agaagtttat tgaggtagtt
      721 tagatgaatc aagaaatatt aaaaaagagt aagattcaat tgacagttga acatattcaa
      781 aaatattgga ttttatattt aatgatgatt ccagcaactg ttctactaat tttatttacg
      841 tatggaccaa tgtacggcat tattatggcc tttcaagact ttactgtctt caagggatat
      901 actggaagtc catttgtcgg tttaaaacat tttcaaagat tgttctcaga tcctttgttt
      961 tatagattgt tcaagaatac gttcatggta ggggttttag actttttatt ctctttccca
     1021 gctccgctta tttttgcact catattaaac gaagtcagaa aagtaagatt taagagtgta
     1081 gttcaatcta ttagttatct tcctcatttt attcccctag tggttatggt tggtattatt
     1141 tttgaactat ttggttcata tggtattatt aacagtttac tttcatcact tggaatggag
     1201 ccaataagtt tctttacaaa atctgaatgg ttcttgcctc tctacattgg ttcaggagta
     1261 tggaaaacaa taggttgggg ttctatcatt tatatgggag cattaaccaa tatagattca
     1321 actttatatg aagcagcaga tatggacggg gctaatagat ggcacaaaat gtggcatgta
     1381 actctaccaa gtttaagacc tacagtagtt actttgttta tactaaatgc tggtggtatc
     1441 atgcaagtag gatttgaaaa ggtattcttg atgtctagtc ctgcaacata tgaagtatct
     1501 gatgtattat ctacttatgt ttatagacaa ggtattttaa attctgattt cagttactca
     1561 gctgc
//
LOCUS       DAVLXF010000163         1540 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLXF010000163 DAVLXF010000000
VERSION     DAVLXF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.163"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            22..1368
                     /gene="gdhA"
                     /locus_tag="VYO12_002110"
     CDS             22..1368
                     /gene="gdhA"
                     /locus_tag="VYO12_002110"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1588626.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGHLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          475 a          302 c          323 g          440 t
ORIGIN      
        1 tgacaataaa aggagaacga tatgacatct gctaaagaat atatccaaag cgtgtttgaa
       61 actgtaaaag ctcgtaacgg gcacgaggct gaattcctcc aagctgttga agaatttttc
      121 aacactttgg aacctgtatt tgaaaaacac cctgagtata tcgaagaaaa tatcttggca
      181 cgtattactg agcctgagcg cgtgatttct ttccgtgttc cttgggttga ccgtgatgga
      241 aaagttcaag taaaccgcgg ctaccgtgtt caattcaact cagctgttgg accatataaa
      301 ggcggacttc gtttccaccc aactgtaaac caagggattt tgaaattcct cggatttgaa
      361 caaatcttta aaaacgtctt gactggactt cctatcggtg gaggtaaagg tggatcagac
      421 ttcgatccta aaggtaaaac agatgctgaa gtgatgcgct tctgccaaag cttcatgact
      481 gaattgcaaa aacacatcgg accatcactt gacgtacctg ctggtgatat cggtgttggt
      541 ggacgtgaaa ttggttacct ttacggtcaa tacaaacgtc ttaaccaatt tgatgctggt
      601 gtcttgactg gtaaacctct tggatttggt ggtagcttga ttcgtccaga agcaactggt
      661 tacggtttgg tttactatac tgaagaaatg ctcaaagcta acggtaacag ctttgctggt
      721 aagaaagtcg ttatttcagg ttctggtaac gttgctcaat atgctcttca aaaagcaact
      781 gaactcggtg caactgttat ttctgtttct gactcaaatg gttatgtcat cgatgaaaat
      841 ggtatcgact tcgatctttt ggttgatgtt aaagaaaaac gtcgtgctcg tttgactgag
      901 tatgcagctg aaaaagcaac tgcaacctac cacgaaggta ctgtatggac ttacgctgga
      961 aactatgaca ttgctcttcc atgtgccact caaaacgaaa tcaacggtga agcagctaaa
     1021 cgtttggttg ctcaaggcgt tatctgtgta tctgaaggtg ccaacatgcc gagcgacctt
     1081 gatgccatca aagtttacaa agaaaatggt atcttctacg gacctgcaaa agctgccaac
     1141 gctggtggtg tagccgtttc agctcttgaa atgagccaaa acagtcttcg cctctcatgg
     1201 actcgtgaag aagttgatgg ccatctcaaa gacatcatga caaacatctt caacacagct
     1261 aaaacgactt cagaaacata cggtcttgat aaagactacc ttgcaggagc taacattgct
     1321 gcctttgaaa atgtagcaaa tgccatgatt gcacaaggta ttgtttaaga tttatttact
     1381 caatcctcaa tcactacgat tggggatttt tagcttgaat taaaaaattg gaaaatctaa
     1441 ttaaataaaa tgaaatgaaa taaaaactga acaaattgat tgcggaattc aaaccaattt
     1501 ctaacatact tctagaaatt aatttgactt ttctaatcga
//
LOCUS       DAVLXF010000164         1511 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLXF010000164 DAVLXF010000000
VERSION     DAVLXF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.164"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(503..1495)
                     /locus_tag="VYO12_002111"
     CDS             complement(503..1495)
                     /locus_tag="VYO12_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588627.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          516 a          254 c          190 g          551 t
ORIGIN      
        1 ttacagaaag aacaaaaatg ctctgaaaca ctgatttcaa agcatttttt tgttagttta
       61 aaattactcc cattcttctt ttccaaacgt acaatatatc caaaaccatt caaaatacta
      121 gattctattt tttataatat cactaaatcc acctaattat aggacgtttt cagattttta
      181 gtcccgtccc agtcccagta ccggagaaat attgttttaa tataatatct ctttttgtct
      241 tctaagctct taaaagcaaa agaacaagta aagagtcaag acaaggataa aaagtccata
      301 ttagggcaaa taaaaagctt taagacagat gacaaatcta agtcaaataa gaaagaccat
      361 agcaaaggtg cagagagata aatattggcg gtcttcggac tgcctttatt tttttatcca
      421 tttttcaaat caaatttatt cagactatat atgcacatat acacttaaat tcatataaaa
      481 acatggcttg taaaaaatta ctttaatcac aataatcgca tttaaaattg tgatgtttgc
      541 aagctaaatt acggacttca cttggaagtt ttcccttgta tcttttataa tagatagaaa
      601 atttgctggc agatgaatat ccaacagatt ctgctatctc ttttataggt agttcagtgt
      661 ttaaaagaag agtttcagct acattcattc tttttctttg agtgtactct gtaatgcttt
      721 gacaatattt ttccttaaat aaatttttta atttagtacc actcatttta gatatttttt
      781 caagcgtgcc ttgatttaca ttcgttgcaa aatgatcatc taagaatctt gctacatctt
      841 caagtgcttt atcatcatca atttcaattt tatatttttt tctatttaag tatgtgtcaa
      901 ttactatact tatccattca tttgcctttg ctttaaagaa aaaatcagcg gcaggagcgt
      961 ccatcttaca atttaatatt tccattgcca ctctttctaa ggcctttgta agtattattt
     1021 gattcggttg aagcaaggtt gaataaaaag attctggatt aatgttaata gatgctaaat
     1081 gtttttctat tagctctttt ttaaaaccaa tggaaacagc aagataacaa caattctcgt
     1141 gtaataaaaa aacaaaatca tcttttatat tatcaaaatc aaaagtacat agagagtttg
     1201 cggtaatagt ttgatacgga ttaaactttt ctccgtttgc actgacaatg taacttgaat
     1261 aaattgaaac atagtctgac atactataag tgctattttg aactacttcc tctttgatat
     1321 aaaaatcatg tatatcgata atgaagctgc ctccttcata aaaccaatat aggccttctg
     1381 cataagttga attctctttt ttccaacaaa atgtatgacc tgcacttgaa tacttctcat
     1441 tgtttttaca ttcatctact ttcatataat cttttacaaa atcataatat gacataacac
     1501 actatccctt t
//
LOCUS       DAVLXF010000165         1459 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLXF010000165 DAVLXF010000000
VERSION     DAVLXF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.165"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            17..1007
                     /locus_tag="VYO12_002112"
                     /pseudo
     CDS             17..1007
                     /locus_tag="VYO12_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            997..>1459
                     /locus_tag="VYO12_002113"
     CDS             997..>1459
                     /locus_tag="VYO12_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588628.1"
                     /translation="MPANTKVIFQETFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYSLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
BASE COUNT          495 a          206 c          280 g          478 t
ORIGIN      
        1 agaataagga aaaaacatga atgtcaaaaa gataatgtca atttttcaat ccttttatgt
       61 tgatgtcagt attgaggaac tgactttacc aatcagtttt gtaaaaaagt ttgagtatac
      121 tcaaatgact tttcataagg aatcattttt attgattaaa gaaaagagaa gggggagttt
      181 gagttcattt gttactcagg ctcgcactat gggtgataaa gccaatatgg atgttgtttt
      241 ggtgttttcg aagttatcag acggtgaaaa aaaagcaatt acttcaagct agagttccgt
      301 ttgtagactt taagggaaac ctcttcttcc ctacattggg actagtactg aatgcgaatg
      361 atactgaagt ccctaaggaa ttaataccta gcgaacaatt aacgtggatt gcctttttat
      421 tgacaaaagg tcaaaaagta gtagatgttg atttgctttc acaagtcact ggacttccaa
      481 actcaacaat ttataggtgt ttgaggactt ttaaagcttt atatttgtta aacaagcaaa
      541 ataagcttta cacatatacg gtgtcaaaga aagaattatt cttaaaatcc gtgtcatgtt
      601 tatttaatcc catcaaaaaa cggattttat tgccagatgg cgatataaag cagataaaat
      661 ctgtttctaa ccttctatat ggtggtgctt atgctttgtc gcattcaact tttttagctg
      721 aaacggatga aaatattagc tatgtcatat ggcagagaaa attcaatcag ttacccttgc
      781 cactttctca gcatatttta aaaggaaaga tgctagagat atggaaatat cgtccttttg
      841 tatctgagtt ttggaatgat tttaaaaata atcatgataa acaatttgta gatccgattt
      901 ctctttattt gaccttaaaa gatgatgatg acccacgtat agaggaagag agtgaagcac
      961 tagaaaatat gatattacag tatctgggag aagatgatgc cagctaatac gaaagttatt
     1021 tttcaagaaa cgtttgcgga ttttcagaac tattatgttc tgattggggg aactgctacc
     1081 tctatcgtat tggattcgca aggatttaaa agtcgcacaa caaaagatta tgatatggtc
     1141 atcattgatg aagtaaaaaa taaggaattt tatactacct tgaatcattt tttagaattg
     1201 ggagagtatc aaggaagtca gaaagatgag aaagcgcagc tttttcgatt tacaacaact
     1261 aatcctgagt ttccttctat gattgaacta tttagtatct taccagaata ttcattaaag
     1321 aaggacggtc gagaaattcc cttacatttt gaccaagatg ctagtttatc agccttatta
     1381 ttggatgaag attattataa tatattggtg catgaaaaag aaaccattca ggggtattcg
     1441 gtattgagta attgtggtc
//
LOCUS       DAVLXF010000166         1449 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLXF010000166 DAVLXF010000000
VERSION     DAVLXF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.166"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            10..735
                     /locus_tag="VYO12_002114"
     CDS             10..735
                     /locus_tag="VYO12_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588629.1"
                     /translation="MLKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILGISGSG
                     KSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIPSLPVI
                     ENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAVIADSD
                     IIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILKDGKIF
                     KELHRPSGEELYKILEVQSTTEE"
     gene            738..>1449
                     /locus_tag="VYO12_002115"
     CDS             738..>1449
                     /locus_tag="VYO12_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588630.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVII"
BASE COUNT          467 a          190 c          285 g          507 t
ORIGIN      
        1 aaagattata tattaaaagt gaaagggctg tatcatcaat ttttactggg aaacaataaa
       61 acgttgcaag tgctgaaaaa tgtttctctt tctgcttcga gaggagaatt tataagtatt
      121 ctaggaatta gtggttctgg aaagtcaact ttattaaaat gtatttctag tttgcttgaa
      181 ccaacaagtg gggaagtaat tttaaatgga atcaacccct ataaaatcag aaatgcaaaa
      241 ttgtcaagta taagacgtaa cgaagtatct tttatatttc aagcatacaa tttaatacct
      301 tccctgccgg taatagaaaa tatagcactt cctttgcgat tatcacaaaa aaaattaact
      361 attaaaaatg tagaaaactt actcaaaaga atgaagttta atgctggctt aaacgatttt
      421 gttggaactc tgtctggtgg agaacaacag aaggttgcta tagctagagc ggttattgct
      481 gatagtgata taatatttgc tgatgagcca actggggctt tagacagcgt ttctcgtgaa
      541 gtaatttttg aattattgag agagttagta ggggcgggta agtgtgtaat tatggtaacg
      601 cacgatatag aattggcctc gaaaactgat cgtgcattaa tattgaaaga tggaaaaatt
      661 ttcaaagaac ttcatagacc tagcggggaa gagttgtata aaatcttaga ggtacaatca
      721 actacggagg aatagagatg ctcagattag tttactatca atttttacat aataagaaac
      781 aatggttagg cgtttcacca gtaatttttg tttcgagttt agttatggga ttggctgtta
      841 atggggtaat taatgttgag aataattcgc aagtatttgt cggacttcca gatcctaaac
      901 caatttttat gtttccgata gtattcggag gagtaacatt attctttgtg ctctctaaca
      961 taataaatat gttggttgaa atttttagag atgattacga acttctggag gtgttgggag
     1021 caagtctcct tcaactatca tttcttgtgg ggggacagat ttttatcatt tcctcgatta
     1081 ttagttttat tgcatatcta tgctctatat ttgttacgtc aaattattat tattttttac
     1141 aatacttttt cggggaaaat atattacctg atatccaatt tcaaacgagt gctgttggat
     1201 gtatcattac agtagtgttg atttcatttt tagcctttct aagtggttgc ttttatacgt
     1261 tcaagaagat tagaaatagg aagagttcta aaataaggca tgttctttca atagtaaaaa
     1321 gaatactgct attggctggt tttagtgtta tctggttgct ttctttgcaa caaattttcc
     1381 aagatagcac aattttagcg aaggcccaga taatattcaa tattgtaatc ttagatattg
     1441 tgattattt
//
LOCUS       DAVLXF010000167         1436 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVLXF010000167 DAVLXF010000000
VERSION     DAVLXF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.167"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>1436
                     /locus_tag="VYO12_002116"
     CDS             <1..>1436
                     /locus_tag="VYO12_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1588631.1"
                     /translation="FSVIIVNCLLAVVCKKINSSSTKLLPEFSGLDFSLWQDYIQSTG
                     SIKELWPSQIELGKQGIFSGKSGIVQMPTSSGKTASINLTLRSAFYSNRIDNALIVAP
                     FRALCREIYRDINAHFVDENNVIVSEVFDLPEIPQDFSIFNDGKKRVFILTPEKLLFL
                     LRNHQSFIDEIGLCIFDEAHLFDDPSRGTNFELLLSTVKQIFPKGIQKILISAVIPNS
                     EAINRWFNEDGVIVSNNSIKTTEKRVAFSDLNGSNEQLYFIDPITFEEEFFVPRTVSV
                     SELEQLGKERKQKVFPELTNANDISIYYGTKLINNGGVGIFCGRKDTVNVILRRFIDL
                     NNRNYDLTDFLKNSDRSEVEKIGNLIGQNLGYDSVEYTCSQLGVFSHHSGIPMGIRIA
                     IEYAFSKSKINNVVCTSTLAQGINLPIKYLIISSVYQAGDAIKVRDFQNLIGRAGRAG
                     KYTEGTIILTEPNIYKSPKNKWKKQNYKA"
BASE COUNT          531 a          172 c          264 g          469 t
ORIGIN      
        1 ttttctgtaa ttattgtaaa ttgtctctta gcagttgtat gtaaaaaaat aaatagttca
       61 tcaacgaaat tattacctga attttcagga ttagattttt ctttatggca ggattatata
      121 caatcaacag gaagcataaa ggagctttgg ccatctcaaa tcgaattagg gaagcaagga
      181 attttttcag gtaaatctgg aatcgttcag atgccaacaa gttcgggtaa aacagcctca
      241 atcaatctaa ctttaagatc agcattttat tcaaatagaa ttgataatgc attgattgtt
      301 gcacctttta gagcattatg tagagaaata tatcgagata ttaatgcaca tttcgttgat
      361 gagaataatg tgattgttag tgaagttttt gatttaccgg agattccaca ggatttctca
      421 atttttaatg atggtaaaaa aagagttttt atacttactc cagagaaatt gttgttttta
      481 ttgagaaatc atcaaagttt tattgatgaa ataggacttt gtatatttga tgaagcacat
      541 ctttttgatg atccaagtcg tggaaccaat tttgaattac tgttgtcaac ggttaaacaa
      601 attttcccaa aaggaataca gaagatttta atatctgctg taataccaaa tagtgaagct
      661 ataaatcggt ggtttaacga agatggagta attgtttcta ataattcgat taaaacaaca
      721 gagaaaagag ttgcgtttag tgatttaaac gggagtaatg agcaattata tttcatagat
      781 cctattacat ttgaagaaga attttttgtt ccacgtactg ttagtgttag tgaattagag
      841 cagcttggaa aagaacgcaa acaaaaagtt tttccagaac tgacaaacgc aaatgatata
      901 agtatttatt atgggactaa gctaattaat aatggtggag taggaatttt ttgtggaaga
      961 aaagacacgg tcaatgttat tttacgaaga tttatagact taaataatag aaattatgat
     1021 ctaactgatt tcttaaaaaa ctcagatagg tctgaagttg aaaaaatagg aaatttaatt
     1081 ggtcaaaatt taggatatga tagtgttgaa tatacatgta gtcagctagg agttttttca
     1141 catcattcag gtatacctat ggggatacgc attgctatag agtatgcttt ttcaaaaagt
     1201 aaaataaata atgtagtatg tacttcaacc ttagcacaag gtataaactt acctattaaa
     1261 tacttgatta tctcaagtgt gtatcaagca ggagatgcaa taaaagttcg tgattttcag
     1321 aatttgatag gtagagcagg aagggctgga aaatatacag aaggaacaat aattttaaca
     1381 gagcccaata tttataaatc accaaaaaat aagtggaaaa aacaaaacta taaagc
//
LOCUS       DAVLXF010000168         1395 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLXF010000168 DAVLXF010000000
VERSION     DAVLXF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.168"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..1054
                     /locus_tag="VYO12_002117"
     CDS             <1..1054
                     /locus_tag="VYO12_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1588632.1"
                     /translation="INKWDNFNTKQLIIGRKLDIHLSIVSSITNVLQIILPVLTLIVG
                     VNIKTFEQLTLGQIVAISTVSPYFISPIISLSDNYIQLMLLKGYFLRIEDVFNTKSEL
                     IPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGETVAIVGESGSGKSTL
                     AKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTFGHFV
                     SDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKPSVVV
                     LDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEIGTHR
                     ELLELGGIYKQLYSNN"
     gene            1078..>1395
                     /locus_tag="VYO12_002118"
     CDS             1078..>1395
                     /locus_tag="VYO12_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1588633.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSE"
BASE COUNT          530 a          170 c          234 g          461 t
ORIGIN      
        1 gattaacaaa tgggataatt ttaatacaaa acagctcatc ataggtcgaa aacttgatat
       61 acatttatca attgttagta gtataacgaa tgttttacaa attattctcc ctgttttgac
      121 ccttattgta ggtgtaaata taaaaacatt cgaacaattg acgttaggac aaattgtagc
      181 aataagtaca gtctcaccat actttatttc tcctataatt tctttaagtg ataactatat
      241 acaattaatg ttattaaagg gatatttttt aagaatagag gatgtgttta atactaaatc
      301 cgaattaatt ccagaaagag tcagtcaaga tataaaattt gataaaaaaa tagaattaaa
      361 agatatttgg tataaatatg gattatttga tgattatgtt ttgaaaggaa taaatgttac
      421 tattaaaaaa ggagaaactg ttgctattgt tggagaatca ggttcaggta agagtacatt
      481 agctaaaatt ttattaggtt tattagaacc taatattggt tcaatagaag ttgatggagt
      541 agaaaaagaa gaaattggtc aaacattgta tagaaagatt tttggagcag tgttacaaaa
      601 ttcaacccta agttatggta ccttaagaga gaatttgaca tttggacact ttgtttcaga
      661 tgaagaatta atgacaaatc taaattcaat tggtcttagc aatgtagtta aatctttacc
      721 tcttggatta gagacaatca tcgctgaaga aggtaataac ttttctggag ggcagcagca
      781 aatgatactt ttagctcgtt gtcttttgtc gaaaccttcg gtagttgttt tggacgaagc
      841 aacaagtagt ttagataatt tatctcaaca aattacaact tcttacttaa gtgaaatcgg
      901 taccactaag attttaattg cccatcgact agatactatc aagtctgcag ataagatctt
      961 agtaatgcat aatggtgaaa ttgtagagat tgggacccat agagaacttc ttgaactagg
     1021 aggcatttat aagcaattgt attcaaataa ttagtttttg attaaaaggg taaatttatg
     1081 aagattatga aaaaaaaata ttggacttta gcgatattat tcttttgttt gttcaataat
     1141 tctgttactg ctcaagaaat acctaaaaat cttgatggca atataactca cactcagact
     1201 agcgaaagtt tttctgaatc tgatgaaaaa caggttgact attctaataa aaatcaagaa
     1261 gaagtagacc aaaataaatt tcgtattcaa atcgataaga cagaattatt tgtaacaaca
     1321 gataaacatt tagaaaaaaa ctgttgtaaa ttggaacttg aaccacaaat aaataacgat
     1381 attgttaact ctgaa
//
LOCUS       DAVLXF010000169         1381 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLXF010000169 DAVLXF010000000
VERSION     DAVLXF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.169"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>1381
                     /gene="pcpA"
                     /locus_tag="VYO12_002119"
     CDS             <1..>1381
                     /gene="pcpA"
                     /locus_tag="VYO12_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1588634.1"
                     /translation="KTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQ
                     NNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQ
                     AFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFD
                     FSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLP
                     KSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKT
                     PKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAF
                     YGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHI
                     KNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVA
                     KPKKNSNQGV"
BASE COUNT          538 a          195 c          222 g          426 t
ORIGIN      
        1 aaaactacaa tattatcatt aactacagct gcggttattt tagcagcata tgtccctaat
       61 gaaccaatcc tagcagatac tcctagttcg gaagtaatca aagagactaa agttggaagt
      121 attattcaac aaaataatat caaatataag gttctaactg tagaaggtaa cataggaact
      181 gttcaagtgg gtaatggagt tactcctgta gagtttgaag ctggtcaaga tggaaaacca
      241 ttcacgattc ctacaaaaat cacagtaggt gataaagtat ttaccgttac tgaagtagct
      301 agtcaagctt ttagttatta tccagatgaa acaggtagaa ttgtctacta tcctagctct
      361 attactatcc catcaagcat aaaaaaaata caaaaaaaag gcttccatgg aagtaaagct
      421 aaaactatta tttttgacaa aggcagtcag ctggagaaaa ttgaagatag agcttttgat
      481 ttttctgaat tagaagagat tgaattgcct gcatctctag aatatattgg aacaagtgca
      541 ttttctttta gtcaaaaatt gaaaaagcta accttttcct caagttcaaa attagaatta
      601 atatcacatg aggcttttgc taatttatca aatttagaga aactaacatt accaaaatcg
      661 gttaaaacat taggaagtaa tctatttaga ctcactacta gcttaaaaca tgttgatgtt
      721 gaagaaggaa atgaatcgtt tgcctcagtt gatggtgttt tgttttcaaa agataaaact
      781 caattaattt attatccaag tcaaaaaaat gacgaaagtt ataaaacgcc taaggagaca
      841 aaagaacttg catcatattc gtttaataaa aattcttact tgaaaaaact cgaattgaat
      901 gaaggtttag aaaaaatcgg tacttttgca tttgcggatg cgattaaact tgaagaaatt
      961 agcttaccaa atagtttaga aactattgaa cgtttagcct tttacggtaa tttagaatta
     1021 aaagaactta tattaccaga taatgttaaa aattttggta aacacgttat gaacggttta
     1081 ccaaaattaa aaagtttaac aattggtaat aatatcaact cattgccgtc cttcttccta
     1141 agtggcgtct tagattcatt aaaggaaatt catattaaga ataaaagtac agagttttct
     1201 gtgaaaaaag atacatttgc aattcctgaa actgttaagt tctatgtaac atcagaacat
     1261 ataaaagatg ttcttaaatc aaatttatct actagtaatg atatcattgt tgaaaaagta
     1321 gataatataa aacaagaaac tgatgtagct aaacctaaaa agaattctaa tcagggagta
     1381 g
//
LOCUS       DAVLXF010000170         1381 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLXF010000170 DAVLXF010000000
VERSION     DAVLXF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.170"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(22..720)
                     /locus_tag="VYO12_002120"
     CDS             complement(22..720)
                     /locus_tag="VYO12_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588635.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLANWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(730..>1381)
                     /locus_tag="VYO12_002121"
     CDS             complement(730..>1381)
                     /locus_tag="VYO12_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588636.1"
                     /translation="RCRQYSIHPNVLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNY
                     FNRLECRIRLKSIKHFTSFSFKLAALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVD
                     FLREFYGISIANNASFFIGFFFSYIAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFV
                     ESLFWIYQLNNGIIGLLPIFQYMVNSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
BASE COUNT          581 a          254 c          168 g          378 t
ORIGIN      
        1 tttttaatgt aattatttca ttcattctat aaacctcctc ttttgacgag tgaaatagaa
       61 aatgctttta aaaagaaaga ctaaaaatag caactgaaga aataaatctc gtcctatatc
      121 tccattccct cgattcaaaa tataaaatag ataattagtt cgatttccta caaatagacc
      181 accaaacaca atcatgagta aaaagaaact aacgcaagca aagttcattt gtacattcgc
      241 tcctagtgcg tataacataa agcaaagcaa ggatagcaca caagcattca aagaatcaac
      301 tataaacaaa actcctatat cccagtttgc taactgtaac ataaattgaa tcactagaac
      361 ttttaccatg attaaaatga gtccataaag aaaacaggtc caaatctgat acctaatcca
      421 gagcttaata gaagaaaatc gaatcacttc cataaagaaa tcggatgctc ttctacgata
      481 tacaactgct agaaaaaatg tatgaataat aaaccaatga agcaaccata tagataagac
      541 taaaaaagag ttttgactca gattaggccc ttttaaaatt tctataacaa gtctatcatt
      601 tccttctaac caataccata atcctaaaca aatcaatatc caaaaaagaa cataacccca
      661 aaagtacttg gtatagatta tctgattcaa cctccacatg tgactactta actttcccat
      721 ttccaacttt tacactctcc tccagtttct atgaacagaa aatacagtca atggaattat
      781 gatagatagt aatgtaagcc aataaatcaa tgcatacgga ttggaattta ccatatactg
      841 aaaaattggc aataatccaa ttatcccatt gttcaactga taaatccaga ataaggattc
      901 tacaaataaa aaagtaaaca gaaatactaa gctcatgttt gattttttaa accaagaaaa
      961 actgctaata gtaagtaagg ataaaaagaa atagtatgct atataagaga aaaaaaatcc
     1021 tataaagaaa ctagcattgt ttgcaatact tataccataa aattctctta aaaaatcaac
     1081 ctcctttatc tccaaagaga agctaaaacc attactaaat gcaattagaa aaatcaataa
     1141 aaataaagtc gccgtccaaa tccccgtact aagagctgct aatttgaaac taaaactggt
     1201 aaagtgctta attgatttca gacgaatgcg acactccaac ctattaaaat agttattcat
     1261 caaataaaaa aagaacaata tatatgtgaa cggaaagcaa tatactccag tcgtcatatc
     1321 ttgaagtaaa actaaaatcc attctaatac atttggatgg attgaatatt ggcgacagcg
     1381 c
//
LOCUS       DAVLXF010000171         1352 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLXF010000171 DAVLXF010000000
VERSION     DAVLXF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.171"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..179)
                     /locus_tag="VYO12_002122"
     CDS             complement(<1..179)
                     /locus_tag="VYO12_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1588637.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADSISKSQLSRFERGENEISL
                     STFFELLSNINVSIE"
     gene            complement(192..965)
                     /locus_tag="VYO12_002123"
     CDS             complement(192..965)
                     /locus_tag="VYO12_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1588638.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAN
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
BASE COUNT          458 a          247 c          144 g          503 t
ORIGIN      
        1 ttctctatcg aaacattgat atttgataat aattcaaaaa atgttgaaag agaaatttca
       61 ttttctcccc tttcaaatct agaaagttga gattttgata tagaatcagc tgcagcatac
      121 tctaaagaat atccgtttaa ttttctaaaa tatcgaaaag cttctccgta atttttcaca
      181 ctttcattcc cttaactttc ttttcgagcg ataaacatac catattcttt aggaatgatc
      241 aacataccat tattaaactc actttccaat cttttacgta ttatttcctc ttctagtgaa
      301 cctatttctg aaattccttt aaacgattgg atgtatttaa ctaggtcatc tatactagtt
      361 acctgtaact catcatcata gagtaatgta tcgacagagt taaaatacct actcaggtac
      421 cttttaccat tttgtaaagt aaacgttcta ttttctaaat cttgattaac ctcatcttta
      481 aataaatttg ccaaataatt tacaactcca ttttcaccaa atgtagcaca gtaaaaaatt
      541 cctccggttt ttaagaccct attcacctca gagagagctt taggaatatc attcacatga
      601 tgtagaagca tattagcaat aacaatatca aaagtttcat tctcaaaaga aatcttttga
      661 atatccatta tctcataatt gacattgttt ctatttccaa tcactgactt cgttgatttt
      721 accatatctt tagaaaaatc tgtaacaatc aactgcttca ttttatctat agaatcacta
      781 ttacttttcc acaattctcc tgtaccacat cccaattcta gaacctttac ttcatcagtg
      841 atttggtagt tagaaaatat ccaattatta aatcccaatt tattttttga atacttctta
      901 tgcaattcta ctcgaatatc aagattatca tgacttttat attgttcaat tactttatca
      961 atcattttaa atgacctcca ctctataatt tggcattata tcacaagaat aaagatagtg
     1021 ttaaataagt tgtataaatg caaaaaagga atattccgtt atagcagagt tgtaacacat
     1081 ttctataaag aaatttattc ctacgactca gtttggcatg cttaacttcc taactcaaaa
     1141 acaaagcatc gattaattta tccgttcttc ttttgaaaca gatttgaact gataattgct
     1201 cgttttaatt taagaagctc ttttgagcaa aacttcattt ttttaagagt ccaacaattt
     1261 ctgtctatcg cttttctact agcaaaaaaa actaccttga ggcaagtttt catttatata
     1321 tctaatttcg taatagtata gtaaaatgaa at
//
LOCUS       DAVLXF010000172         1350 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLXF010000172 DAVLXF010000000
VERSION     DAVLXF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.172"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..334)
                     /locus_tag="VYO12_002124"
     CDS             complement(<1..334)
                     /locus_tag="VYO12_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEV1588639.1"
                     /translation="MTLRIKYIHEIFLEEIDKNMKKVLIVRSGPYQVNPEGYNLQELG
                     LASAISQQGYQCDVMYYHKTKNYDQLYEKNGEQIKIFWRRGIRLFRSGIYPQILREKF
                     LNQYDKIII"
     gene            complement(285..>1350)
                     /locus_tag="VYO12_002125"
     CDS             complement(285..>1350)
                     /locus_tag="VYO12_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV1588640.1"
                     /translation="NLDLVATIDAETAYKDASFIVVATPTNYDLERNFFDTSTVESVI
                     QQVQNMNSTATIVIKSTVPVGFTDSIRKKLGISNLLFSPEFLRESRALYDNLYPSRII
                     VGTDLSNQKLVEKAQVFSNLLVQGAIKKDIPILTMGFTEAEAVKLFANTYLALRVSYF
                     NELDTYAEIKGLDTQKIIEGVSLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYKNVP
                     EELIGAIVRSNTTRKDFIANQVLKVVNYNIPEDDIPTKQMEKEVVIGIYRLTMKSNSD
                     NFRQSSIQGVMKRIKDRGVKVIIFEPTLKDSDTFFGNAVINDLEEFKVKCDVIIANRY
                     DETLNDVKDKVYTRDIFRRD"
BASE COUNT          417 a          250 c          183 g          500 t
ORIGIN      
        1 aaataattat cttatcatac tgattcaaaa atttctctct taaaatctgg ggataaattc
       61 cacttctaaa taatcttatt cccctcctcc agaatatttt aatttgttcc ccatttttct
      121 catataattg atcatagttc tttgttttgt gataatacat gacatcacac tggtagcctt
      181 gctgactgat agcggaggct aaaccaagtt cttgaagatt atatccttcc ggattgactt
      241 ggtaaggtcc acttcgaaca attaatactt ttttcatatt tttatcaatc tcttctaaaa
      301 atatctcgtg tatatacttt atccttaacg tcattcaacg tttcatcata acgatttgca
      361 ataatcacat cacactttac tttgaactct tctaaatcat ttataacagc gtttccaaag
      421 aatgtatcac tatcttttag agtaggctca aaaatgatta ctttaactcc tctatcctta
      481 atacgtttca taactccttg tattgaactc tgacgaaagt tatcactgtt acttttcata
      541 gttaatcggt atattccaat aacaacttct ttctccatct gtttagtagg aatatcgtct
      601 tctggtatat tataattcac cactttaaga acttgattag caataaaatc tttacgtgtt
      661 gtattgcttc gtactatagc tccaataagc tcttctggga cattcttata attggctaaa
      721 agttgcttag tatcctttgg taagcaatat ccaccatacc caaagctcgg attattataa
      781 tgggttccga tacgaggatc taaactaaca ccttcaatta ttttttgagt atctaaccct
      841 ttgatctctg cataagtatc taattcatta aaatatgata cccgcaatgc taagtaggta
      901 ttagcaaata atttaactgc ttcagcttca gtaaatccca tagttaaaat agggatatct
      961 tttttaatgg caccttgcac taaaagatta gagaaaactt gggctttttc aactaatttt
     1021 tgattactta aatctgttcc aactatgatc cgacttggat ataagttatc ataaagtgcc
     1081 cttgattcac gcaaaaattc aggactgaat agaagatttg aaattcctaa ttttttacga
     1141 attgaatcgg taaagcccac ggggactgtt gattttatta ctatagttgc tgtagaattc
     1201 atattctgaa cttgttgaat gaccgattca actgttgatg tatcaaaaaa atttctttct
     1261 aagtcataat ttgttggtgt cgctactacg ataaaagagg catctttata agctgtttca
     1321 gcgtctattg ttgcaactaa atctaaatta
//
LOCUS       DAVLXF010000173         1347 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVLXF010000173 DAVLXF010000000
VERSION     DAVLXF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.173"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            33..872
                     /locus_tag="VYO12_002126"
     CDS             33..872
                     /locus_tag="VYO12_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1588641.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            978..1253
                     /locus_tag="VYO12_002127"
     CDS             978..1253
                     /locus_tag="VYO12_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1588642.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          446 a          210 c          288 g          403 t
ORIGIN      
        1 aatctgaatc ctaaaataga aagattgata ctatgacaaa aattaagatt gtaaccgatt
       61 catctgttac tattgaacct gaactagtaa agcaattaga tattacaatt gttccattat
      121 ctgtaatgat tgataatgtt gtttattctg atgcggattt gaaagaagaa ggtaaatttc
      181 ttcagttgat gcaagaaagt aagaatcttc cgaaaacaag tcagccacct gtaggtgtct
      241 ttgctgagat ttttgaagac ctatgcaaag atggtggcca gattcttgct attcatatgt
      301 cccatgctct ttcgggtacg gtagaagcag cacgccaagg tgctagccta tctactgcag
      361 atgtgactgt tgttgatagt tccttcactg accaagccct gaaattccaa gttgttgagg
      421 ctgcgaagtt agctcaagaa ggtaaagata tggaggcaat tttatctcat gtagaagagg
      481 ttaaaaacca tacagagctt tatattggtg tttcaacttt ggaaaatctt gtcaaaggtg
      541 gacgaattgg ccgtgtaact ggcttgttga gctcccttct caatatccgt gttgtcatgc
      601 aaatgaaaga ccatgaattg cagccaatgg ttaaaggtcg tggaactaaa acatttaaaa
      661 aatggttaga tgagttgata acatcgcttt ctgaacgtgc tgtagcagag attggaattt
      721 catattctgg tagtgatgat tgggcaaaag agatgaaaga aagcttacaa gcttatgttg
      781 aaaagccaat ttctgttttg gaaacgggtt ccattattca aactcacacg ggtgagaacg
      841 cttgggctat tttgatacgt taccactcct aaaaaataaa taaaattgga agaaatagcg
      901 tttttaactt gacctaaaag ggattttagg atatgattat atttgttaat tagaaattat
      961 ctggaggaat cattaacatg gcaaacaaac aagatttgat cgctaaagta gcagaagcta
     1021 cagaattgac taagaaagac tcagcagcag cagttgaagc tgtatttgca gcagtagctg
     1081 actatcttgc agctggtgaa aaagttcaat tgatcggttt tggtaacttt gaagttcgtg
     1141 agcgtgcaga acgtaaaggt cgcaacccac aaactggtaa agaaatgaca attgcagctt
     1201 ctaaagtacc agcattcaaa gctggtaaag ctcttaaaga cgctgttaaa taatcagtct
     1261 ttaaaaagcc tattgtatca agctttctag cttggtcagt aggcttttta attatgaaat
     1321 ctagtaataa atagagttaa aaagttg
//
LOCUS       DAVLXF010000174         1332 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLXF010000174 DAVLXF010000000
VERSION     DAVLXF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.174"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..>1332)
                     /locus_tag="VYO12_002128"
     CDS             complement(<1..>1332)
                     /locus_tag="VYO12_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1588643.1"
                     /translation="IPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVDTSYSE
                     APEDIEGQISTVESIFKQRANSLEAGVNRLTEGLRTKVDISALNVTAENIRQSVKSLE
                     TDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKASKSELTQTAEELASKIASVHLGR
                     RNLLKGTKELARYKPVSEYNGFKVIRTVAGATRYQDSYVERTVIPTAGTEYIAIFYAR
                     ASENDYPVRCHFYNPNTVVSSENSSGYKSRSSDGLSIIRLSTDWQLCWVKWTQTATDQ
                     AKTVIIGRHGPQVGGKEGVWVEICAPAIFEGNLAGDWSPAYEDQDERVSAVESNFKQR
                     ADSLEAGVSRLTEGLRTKADISALNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVR
                     AGSIRQEILNATKDKASKSELTQTAEELASRIASVQASGRNLFLNSLFKQDI"
BASE COUNT          299 a          337 c          276 g          420 t
ORIGIN      
        1 aatatcctgc ttgaatagtg agttcaagaa tagatttcga ccggatgcct gcacactcgc
       61 tatccgacta gccagctcct cagctgtctg cgtgagttct gacttgctag ctttatcctt
      121 ggttgcgttc aagatttcct gacggataga gccagctcgc acctcaaatt cagcctgact
      181 caacttctga tttagtttgt tctgcgtgtc tgtctcaaga ctcttcacag attgcctaat
      241 attctcagca gtcacgttga gtgcgctgat atcggcttta gttctaagcc cttcagtcag
      301 acggcttaca ccagcttcga gtgaatcagc acgctgttta aagttggatt cgaccgctga
      361 gacacgttcg tcttggtctt cgtatgctgg tgaccagtca cctgcaagat ttccctcaaa
      421 aatggcaggg gcgcagattt caacccatac cccctcttta ccgcctactt gagggccatg
      481 gcggccaatg atgaccgtct tggcttgatc tgttgcggtt tgggtccatt taacccagca
      541 caactgccag tctgtcgaga gacggataat agacaagcca tctgacgacc ttgacttata
      601 tccgctgctg ttttctgatg atacaaccgt gttaggattg taaaaatgac agcgcacagg
      661 atagtcattt tcactggctc gtgcataaaa gatagctata tactctgtcc cagccgttgg
      721 tataacggtt ctttccacat agctatcctg atatctagtt gctcctgcga ctgttctgat
      781 aactttaaaa ccattatatt cactaaccgg cttgtatctc gcaagctctt ttgtgccttt
      841 cagcagattt ctgcgcccta agtgcacact cgctatctta ctagctagct cctcagctgt
      901 ctgcgtgagt tctgacttgc tggctttatc tttggttgca ttcaggattt cctgacggat
      961 agagccagct cgcacctcaa attcagcctg actcaacttc tgatttagtt tgttctgcgt
     1021 gtctgtctca agactcttca cagattgccg gatattttca gcagtcacat tgagtgcgct
     1081 gatatctact ttggttctaa gcccttcagt cagacgattc acaccagctt cgagcgagtt
     1141 ggctcgttgt ttgaagatcg attctactgt tgaaatctga ccttctatat cttcaggagc
     1201 ttctgaataa gaagtatcta catcgcttat ttcaaacttc ggcatccaaa tccaaacggt
     1261 tccttcctgg ttgaaattga acaaccattc atttgtggtc tgcttagatt cgtttgtcca
     1321 accttttgga at
//
LOCUS       DAVLXF010000175         1319 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLXF010000175 DAVLXF010000000
VERSION     DAVLXF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.175"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            98..>1319
                     /locus_tag="VYO12_002129"
     CDS             join(98..437,437..>1319)
                     /locus_tag="VYO12_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1588644.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKEIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQY"
BASE COUNT          473 a          223 c          292 g          331 t
ORIGIN      
        1 tgaagataaa gttcagatct atgaactaag aaagcaagga caaagcttca aacagctttc
       61 aaaaagattt ggtgtggatg tttctggtct aaagtacatg gtgaaattaa ttgaccgtta
      121 tggcatagaa atcgttaaaa aagggaagaa tcgtcactat tcttctaaat tgaaacaaga
      181 aatgatggat aaagccttac tggaaggttg ttcacaaaga agtattagtc ttgattatgc
      241 cctcccaaac caaggaatgc tttcattttg gccggcacaa tacaagaaaa acgggtatac
      301 tattgttgag aaaacaagag ggagaccagc taaaatggga cgtaaacgga agaagacttg
      361 ggaagaaatg acagaactag aacgactcca ggaggagaat gaacgcttac ggactgaggt
      421 ggcctaccta aaaaagttaa aagagttaga ggaaagggac gaagccctag agcgagaaag
      481 gcagagacag ttagagaaat ggtttcagga ggatttcgac tagattttct tcttgaaaca
      541 gcacgtttag ctcgctcgac ttactattat cagttgaaac aactggatgg ggttgataaa
      601 gataaagaga ttaaaaccga aattcagggc attgataatg aacataaagg caactatggc
      661 tatcgtcgaa tccatttaga actaaggaat cgtggttttg tggtcaatca taagaaagtt
      721 caacggttga tgagaattct tggtttaacg gctcgaattc gccgtaaacg gaagtattct
      781 tcctaccaag gagagattgg caagaaagca gagaatctca ttcaacgcca gttcgaagca
      841 tcgaggccaa tggaaaagtg ctatacagat gtgacagagt ttgccattcc aaatagcaca
      901 cagaaactct atttatcacc agttttagat ggttttaaca gtgaaatcat tgcttatcat
      961 ctttctactt cgccaaactt agaacaagtg aagagtatgt tagaacaggc ctttacagag
     1021 aaatactatg agaatacgat tctccatagt gaccaaggct ggcaatacca acacgattct
     1081 tatcatcggt tcctagagag taaggaaatt caagcatcca tgtcacgcaa gggcaacagc
     1141 ccagacaacg gcatgatgga atctttcttt ggcattttaa aatccgaaat gttttatggc
     1201 tatgagaaga actttagatc tttagaaaac cttgaacaag ctattgtgga ctacattgat
     1261 tactacaaca acaaacgaat taaggtaaaa ctaaaaggac ttagttctgt gcagtacag
//
LOCUS       DAVLXF010000176         1281 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLXF010000176 DAVLXF010000000
VERSION     DAVLXF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.176"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..795
                     /locus_tag="VYO12_002130"
                     /pseudo
     CDS             1..795
                     /locus_tag="VYO12_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(830..1039)
                     /locus_tag="VYO12_002131"
                     /pseudo
     CDS             complement(830..1039)
                     /locus_tag="VYO12_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1205..1278)
                     /locus_tag="VYO12_002132"
     tRNA            complement(1205..1278)
                     /locus_tag="VYO12_002132"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1242..1244),aa:Asn,seq:gtt)
BASE COUNT          423 a          267 c          204 g          387 t
ORIGIN      
        1 agaattcttc ttagaaaagg ccgttttaag tgctatcact gttcaaaaat gagggtcgct
       61 gaaacttcta tcgtcaagaa gaatcatcaa attccttgta tcatcaacca aaagattgct
      121 cagaagctaa ttaaaaagac ttctatgacc gacattgatc atcagttgtc tatttcaact
      181 tcaactgtca ttcgcaagat caatgatttt cactttgagc atgatttttc gcgtcttcct
      241 gagattatgt cctaggacgt tgaaacagtc cggggagtga ctgtttcaat cgggagatgg
      301 agatgagctt tattgctcaa gactttgata agctcaatat cataactgtt cttgagagca
      361 gaacacaagc catcatccga aatcccatga atacaaggct atcaagcgat actgaaagct
      421 cattcaacaa gatagtcaga aactgagtga taaacgattt tatcgcccta cttttcgtat
      481 gcacttgact aataaagaaa ttctagataa acttttgagc tattccgaag acttgaaaca
      541 ccactataat ctctatcagc tcttgctttt ccacctcttt ttcagactct gtttaaaacc
      601 ttcctcaaag ataaagagaa aatcgtcaac gcccttcaat taccttattc caacgccaaa
      661 ctggaagcca ccaataatct catcaaactt atcaagtaca atgcctttgg ttttaggaac
      721 tttgaaaact tcaaaaaaga aaggacgaaa tttgtccttt ctagatcttc gctttcttca
      781 acccactaca gttgacaaag aaccgaaaaa tgtctcgcct ctaatatagt caattgaaac
      841 aagaacaaga caaaagagcc tcataaaagg tattgcaact tgctaatacc tttttggggt
      901 tctttttgat atgaacccat gttttctcaa taggattgta ctcaggtgag tagggaggaa
      961 gaggtaaaag tttatgccca aactcttcac acaagagttc tagcttaccc attctatgga
     1021 atcttgcatt gtccataata tagtagattg aaactagaat agtacacctc tacttctaaa
     1081 acattgttag aattcgattt tactgtcctg atcgatttgt cctattctta tttcatttta
     1141 ctataacacg aaatagatta ttattacata acaaaaaaga ggtctaaacc tcttaactaa
     1201 attactccgc cagtaggact cgaacctacg acatcatgat taacagtcat gcgctactac
     1261 caactgagct atggcggatt a
//
LOCUS       DAVLXF010000177         1261 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLXF010000177 DAVLXF010000000
VERSION     DAVLXF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.177"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            96..>1261
                     /locus_tag="VYO12_002133"
     CDS             96..>1261
                     /locus_tag="VYO12_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1588645.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALFYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHT"
BASE COUNT          472 a          232 c          188 g          369 t
ORIGIN      
        1 aagtaatgaa aagcttaaaa agattaacat ctacatttat ccatagcaaa aaatcatagg
       61 attttaagaa tctgacaact aggaagaaaa ctaatatgac cttatctaag aaacaattac
      121 aactaagagc taaaatactg gaaacagttt acacattagg tcccatttca cgaattgaaa
      181 tcgctactaa aacaggaata acacctgcaa caactagcag tatcacaaat gatctgatta
      241 aagaaaatat tctcttagaa cttggtgaag acgaacacga tacaagcgta gggagaaaaa
      301 aaatcctgct agacattcaa gctaaacgat tttactacat tggttgtgaa ctatctgaaa
      361 aacatttcac ttttgcttta ggagataatc taggaaatat cttaaaggaa gagaaagaaa
      421 ttgttactaa acagttaatc caagaaaagg gaaatcaact cattaaccag acactaaagc
      481 aattcttaaa taactgctct gattatgaaa tagaagcaat tggaatcgcg ctacctggtc
      541 gctatttaga tgattataaa ataacaacaa acaatccttt atggcaacat atcgacttag
      601 aaatgattca atcccacttt gataagcctt tgtttttctc taacaatgtt aactgtatgg
      661 ctataggtaa acgcctattt agtcgccaac agaatgaccc taattttgct tattttcatt
      721 ttgctagagg tatgcactgt tcttatatct acgacggaaa catttatggg aaaggaaatc
      781 taatgattgg agaaataggg catacagttg tttcatctga gggagaagaa tgtagctgcg
      841 ggcgcaaggg atgcttacaa acttttgctg gagaatcttg gttaatcaaa aaatcaaaaa
      901 ttctatatca tcaatctccc tattctctac ttcctagttt agtcaaaaat gccgatgata
      961 ttgatatcca agttatttta accgcttatc aattaggtga cactggaata atcacactta
     1021 tccatcaagc cttattctat cttagtcaaa ccattttaaa tatcagtatg atgattgact
     1081 ctcaaaaaat ctacctacat agtcctttac taacaaatca acacatcatc caaaaactat
     1141 atagtgaaat gaactacaaa cccaaactgc tctataatcg cctacctgaa gtcattattg
     1201 aaccttacaa tgattttaca gccgcacact cagctattgc actttgtttg tatcatacaa
     1261 t
//
LOCUS       DAVLXF010000178         1192 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLXF010000178 DAVLXF010000000
VERSION     DAVLXF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.178"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            14..862
                     /gene="dprA"
                     /locus_tag="VYO12_002134"
     CDS             14..862
                     /gene="dprA"
                     /locus_tag="VYO12_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1588646.1"
                     /translation="MKITNYEIYKLKKSSLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPDSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
BASE COUNT          361 a          206 c          258 g          367 t
ORIGIN      
        1 aatggagtta tttatgaaaa tcacaaacta tgaaatctat aagttaaaaa aatcaagttt
       61 gaccaatcaa cagattttga aagttctaga atacggtgaa aatgttgatc aggagctttt
      121 gttgggtgat attgcagata tatcaggttg ccgtaatcca gccgttttta tggaacgtta
      181 ttttcagata gacgatgcgc atttgtcgaa agagtttcaa aaatttccat ctttctctat
      241 tttagatgac tgttatcctt gggatttgag tgaaatatat gatgcgcctg tacttttatt
      301 ttacaaggga aatcttgacc tcctgaaatt cccgaaggta gcggtcgtgg gcagtcgtgc
      361 ttgtagcaaa cagggagcta agtcagttga aaaagtcatt caaggcttgg aaaatgaact
      421 ggttattgtc agtggtctgg ccaagggcat tgacacagca gctcatatgg cagctcttca
      481 gaatggcgga aaaaccattg cagtgattgg aacaggactg gatgtgtttt atcctaaagc
      541 caataaacgc ttgcaagact acatcggcaa tgaccatctg gttctaagtg aatatggacc
      601 tggcgaacaa cctctgaaat ttcattttcc tgcccgtaat cgcatcattg ctggactttg
      661 tcgtggtgtg attgtagcag aggctaagat gcgttcaggt agtctcatta cgtgtgagcg
      721 agcaatggaa gaaggacgcg atgtctttgc tattcctgat agcattttag atggactatc
      781 agacggttgc catcatttga ttcaagaagg agcaaaattg gtcaccagtg ggcaagatgt
      841 tcttgcggaa tttgaatttt aaaaatgacc taagctagaa ttctaagaaa aaatcaattt
      901 taagagaaaa tgaacccaac atttccataa taaaacgcat attagcaagt ttttaacact
      961 tgataatatg cgttttttct aagtggatta gtagagtaga ggatttttct catataatac
     1021 tcttcgaaaa tctcttcaaa ctacgtcagc ttccatctgc aacctcaaaa cagtattttg
     1081 agctgacttc gtcagtctta tctacaacct caaagcagtg ctttgagcaa cctgtggcta
     1141 gcttcctagt ttgcgctttg attttcattg agtataaggg aaagtatagt ga
//
LOCUS       DAVLXF010000179         1187 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLXF010000179 DAVLXF010000000
VERSION     DAVLXF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.179"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..1116)
                     /locus_tag="VYO12_002135"
     CDS             complement(<1..1116)
                     /locus_tag="VYO12_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588647.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKL"
BASE COUNT          426 a          202 c          144 g          415 t
ORIGIN      
        1 taattttaat aatgtagatt tcccgcttcc actttcacct aaaattaaat acttttcatt
       61 acgttgaaaa caaaaattta agttttttaa tatttcttta tctccatact tatagcaaat
      121 attttttgct tcatataacg gaaaatctct attcacctca tttggttcga tatcattcat
      181 tttatttgac tcaattggat taattgaata caattttaaa aaaataggct tcgtaccaat
      241 aatagaggat aattgacctc ctaattcacc tagcgctgta aaaataacac ctgttagtgc
      301 tcctattgct tcaatagtac caattttcac tattcctttt attgcaagat agcctgttaa
      361 aaaaacgaga gatatctgaa aaaaaatatt gagaaagaag ctaatagcgc ctgctaacgt
      421 ttctacagtt gtctttcttt gtataaccat ctttaataaa attcctgctt ctttaatttt
      481 cttaggcaat acatataaaa gattcaagga cgctaacaca tcaaatccat tcaatatagt
      541 ctcactagat tttaaaaaag cttcattttg gttagttaaa tttagactaa cttctcgcat
      601 tttcgatgca aagatttttg gtacaagtag cataatcatt aatgaaaaca aggtggctac
      661 agtcaatgac caatgatagt gattaagagt cacaactgca aatatagtac cagaaattcc
      721 ttttattact aaaaaaagtt gtttaaaagc ctgatcattt aaagtctgaa catcattatt
      781 tagccacgaa agatatgttc ctgatgattt actatgaaat tcttgatagg tagagttaga
      841 gatgtctgtg gcaactctat ttcgaatctc tagattaaac tcttggatca cttcaacctg
      901 ataatttttc actacccagt caaggaatat tatcccacac cagacaatca tttggtagat
      961 tgacaatttc aaaaaccgct ctaaattcat cgcaattaat tcattcaaca ccagagcatt
     1021 aatagttgct gcataaatta gcaataattg accagcaaca ataaatatcg ttaataaact
     1081 aaattttttt atatttgatt ttataatagt atacacaata gtttctcact ttctaaattt
     1141 taattgaaca tagttgtcat atatacaata gaaaaaacca aaatgat
//
LOCUS       DAVLXF010000180         1146 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLXF010000180 DAVLXF010000000
VERSION     DAVLXF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.180"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            24..>1146
                     /locus_tag="VYO12_002136"
     CDS             24..>1146
                     /locus_tag="VYO12_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1588648.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNS"
BASE COUNT          427 a          144 c          214 g          361 t
ORIGIN      
        1 cattagtaaa aggagttaga ttgatgaata aaagaaaagt tagtttagaa gatttttata
       61 aatggtatag tctaaataaa gaagagttat taaataaggc aactgttggt gaaaagttta
      121 atgataaatt aaaagaagag tttctccagg aatggccttt ggataggatt ttaacaatgt
      181 caatcgatga atatgtaata ggaaagggac agcaaaataa gtctttatgc tacgctcttg
      241 agaagggaaa atacaaaaat ctatttcttg gaatttctgg tggctcagct tcaaaatttg
      301 gtatttattg gaataaaaaa acaaacaaat ataaagatca agctaataat gagatttcag
      361 agttggatca gcgattttca aaattaaaat cagatttgta tgaaattatc aaagaaggta
      421 ttcgttttaa ctttgaaaat cctatttttg atatgaaaag atcaacaaat gaatttattg
      481 gtcgttctgc tatggtgaca aaattacttt gtatctatac tgagggagat cctttctttg
      541 gtgtaaatat taatagtcag aaagaatttt ggaaccactt tgtttctcag acaaatcaag
      601 gtggacctta tctgcaaaat cataaaataa ttgaactggt gtccaaaact tatcctgagt
      661 tggagccatc gaaattagga actatgcttt ttgagtattc taagcttttt atggaaaata
      721 aggaagacaa tagtacaatg gattcatcaa acaattttag tcatcaatta actcaatctc
      781 tattaaagtc tccaaacctt atccttcgtg gtgctcctgg cacaggaaag acttatcttg
      841 ctaaagaaat tgccaaagaa ttaacggatg gcaacgaaga ccaaatcgga tttgtacaat
      901 ttcacccatc ctatgattat acggattttg tagaaggttt aagaccagta tcaaatgggg
      961 atggagctat tgagtttagg ctacaggacg gtatttttaa agatttttgt cagaaagcaa
     1021 aagaaaccca attgattgga ggacaagata attttgatga ggcttgggat tcttacttag
     1081 aatatataaa tgttgctgaa gaaaaagaat atataacaaa aacatcttac ttatctgtta
     1141 atagta
//
LOCUS       DAVLXF010000181         1142 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLXF010000181 DAVLXF010000000
VERSION     DAVLXF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.181"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..669
                     /locus_tag="VYO12_002137"
     CDS             <1..669
                     /locus_tag="VYO12_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588649.1"
                     /translation="STLIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSP
                     IIPESITVADLVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQ
                     RQRVWIALALAQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINL
                     TARYADYLFAIKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKH
                     HVNS"
BASE COUNT          470 a          188 c          180 g          304 t
ORIGIN      
        1 tcaacactca ttaaaacctt gtctcgactt ataaagccat tagagggaga agtattgctt
       61 gataataaat caattaattc ttataaagaa aaagatttag caaaacacat agctatatta
      121 cctcaatctc caataatccc tgaatcaata acagtagctg atcttgtaag ccgtggtcgt
      181 ttcccctaca gaaagccttt taagagtctt ggaaaagatg accttgaaat aataaacaga
      241 tcaatggtta aggccaatgt tgaagatcta gcaaataacc tagttgaaga actttctggg
      301 ggtcaaaggc aaagagtatg gatagctcta gccctagccc aagatacaag tatcctactt
      361 ttagatgagc caactactta cttggatatc tcatatcaaa tagaactatt agacctcttg
      421 actgatctaa accaaaaata taagacaacc atttgcatga ttttgcacga tataaatcta
      481 acagcaagat acgctgatta cctatttgca attaaagaag gtaaacttgt tgcagaggga
      541 aagcctgaag atatactaaa tgataaacta gttaaagata tctttaatct tgaagcaaaa
      601 attatacgtg accctatttc caattcgcct ctaatgattc ctattggcaa gcaccatgtt
      661 aactcttaat aattgaatat tggaattcat ttaaaagtcg caaattttga actgaccctc
      721 aaaagttagg ccaaaaaact aactaaagga ggtcagtttt ttaatggcaa atacagcaca
      781 gaatttaaga taaaattagt aaaagaatat catgaatgaa aaataggata tagagaataa
      841 cagcaatatt aaaaagatca ggactgataa taaacaaaaa gaaagtacaa cgactagttc
      901 aaaagctaaa acttcaagta aaaagttatt caagaaaatc tagaaaatac tcatcctaca
      961 aaggacaggt aggaaaaata tcagacaaca aaataaaaag aaatatttat taccgaaaaa
     1021 aaggggaaat cgagttaagt accaacaggc tcatctattc caaaaagata tttactcgat
     1081 ggaaaaactc ctagaattag tgctacaaat attgacaatg gtatcttagg ttactacgaa
     1141 ga
//
LOCUS       DAVLXF010000182         1082 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVLXF010000182 DAVLXF010000000
VERSION     DAVLXF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.182"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            11..1021
                     /locus_tag="VYO12_002138"
     CDS             11..1021
                     /locus_tag="VYO12_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin formation protein"
                     /protein_id="HEV1588650.1"
                     /translation="MVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFM
                     YYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLA
                     CLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLG
                     DTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIH
                     SLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELES
                     LLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLL
                     EI"
BASE COUNT          375 a          134 c          200 g          373 t
ORIGIN      
        1 tgcagactct ttggttatta ataatattta tgacgaatat ccaatttgga gtatacctgt
       61 taattttaaa ggttctaaat ggtgtcttga acaaacgggt aatgatatgt ataggggtag
      121 agctggaata ttactgttta tgtactatgt aaaattattg aaagataaat catcatatac
      181 tcaactgtac aataaaatgt tatctgcaat tccgccatct gtttctttat ctaagaccac
      241 atttggttta actggggata taggatattt cattgttcta tccattatag aggattacga
      301 tacaaatact ctcgcatgct taaattatat aaagagtgtt ttaactgagc ttgaaaagac
      361 tgattttgca agtgtttcaa acaagtctga ctatattaat ggtcttctac ctttaattaa
      421 tacactggta agatattata ggagaggaat agagaaggaa cgtgttctaa ggctagtatt
      481 aagtttaggt gatacgctat ataatgatgt aagcgagaga gataatagta attttggata
      541 tagttttggc catggactat caggtgtaat ttttacatta aaaaatattt ataaagtgac
      601 aagacttaaa aaatataaag aattaatttt acaactagtt cctagggaga aaatcaaaat
      661 tcattctttg aagtggtgta gtggagaatt tgggacatta gttaatcatc cccaaagtgt
      721 tccagaggga attgtagcta gtttagataa tgatacattt tgccatggaa gtatggccat
      781 aataaatttt tacatcgact acatgaaaga aatggatact agtatggaat tggagtcttt
      841 attgtgtagt gttgtaatga ataaatcaaa agaaggagag tatcgtatcg tccaaacaag
      901 caatttccca gatttttctt tgtatacagg tgtgactggt attgcttata cttttttaag
      961 gtatatcgca ctgaatcaga atgacaaaaa gatagttatc ccctcattac ttgaaatcta
     1021 gcttaattta tagttttgtt tctaaaatag aggtgtccac tcattaagca atataaaaat
     1081 ac
//
LOCUS       DAVLXF010000183         1077 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVLXF010000183 DAVLXF010000000
VERSION     DAVLXF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.183"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..>1077)
                     /gene="lanM"
                     /locus_tag="VYO12_002139"
     CDS             complement(<1..>1077)
                     /gene="lanM"
                     /locus_tag="VYO12_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEV1588651.1"
                     /translation="NLENDFSVLEEELGLRGKLNDIKFGKGDTHSQGKTVLILFFDDA
                     KIVYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEEFIDYLPLEQKKK
                     LPEYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQSLL
                     DTLFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIG
                     KIDFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLK
                     TRVLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLL
                     DIPIFYTDTNINEIYDDFGNHFISL"
BASE COUNT          360 a          206 c          120 g          391 t
ORIGIN      
        1 tagggaaata aaatgatttc caaaatcatc gtagatttca ttgatatttg tatctgtata
       61 aaaaatcgga atatctaata acctcatttg ttcacattcg cttgctacaa taccttgatt
      121 agaaaactta ttgctccaga gattttccaa tgctttttct ctatctaaca tttcttcata
      181 aaaatcagga tgatataaaa aagatagtac tgaagcatag ctatttgtgt ctctaaaaag
      241 tacccttgtc tttaaaccat acaagtttgc ttttaatagc attttaaatt cttctgtttt
      301 atttaactct tcaaatatca gataaaaatc cctaaaacct tttttgaaat cttttatata
      361 cttatcaaat tctatatcac catcccgaac aggcaggttt ttcccacctt caaaatcaat
      421 tttcccaata tcaaacttta ccttatcagt atttaaatta attaaaactt gaccattaaa
      481 agcatccttc ttaaaatttc ctgtaagagc actcatctca acatcaagac catcctccct
      541 ttttactccc tctgttggaa gaagcaaagt tcgagtcacc ctgtgaaaca gggtatccag
      601 caaactctga ccagttttat catcaaatag aggttgctgt aacattgttt caaagtctat
      661 tattacaggc atatctccat aggaaattaa attttcaaaa tgtatatcac tcccattaaa
      721 taaatatata aatgctaaaa gcacaccaaa attataataa tattcaggta attttttctt
      781 ttgctctaga ggtagataat caataaattc ttcatagctg tgatccgaat aagcaatagt
      841 tcgaggtatt cttatcctaa ttttttcatc aacctttcgg atatactcag caatagtatt
      901 tagtgagtta ttgattatta aatttttagg cttgtaaaca attttcgcgt catcaaagaa
      961 gagtatcaaa acagtttttc cttggctgtg tgtatcaccc tttccaaatt ttatatcatt
     1021 taatttcccc cttagcccta attcttcttc tagcacacta aaatcatttt ctaaatt
//
LOCUS       DAVLXF010000184         1063 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVLXF010000184 DAVLXF010000000
VERSION     DAVLXF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.184"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            15..1040
                     /locus_tag="VYO12_002140"
     CDS             15..1040
                     /locus_tag="VYO12_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1588652.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
BASE COUNT          419 a          147 c          180 g          317 t
ORIGIN      
        1 tttaaggagt ttttatgaaa aacaaatttt ttctaatagc tattttagct atgtgtatag
       61 tttttagcgc ttgttcttct aattctgtta aaaatgaaga aaatacttct aaagagcatg
      121 cgcctgataa aatagtttta gatcatgctt tcggtcaaac tatattagat aaaaaacctg
      181 aaagagttgc aactattgct tggggaaatc atgatgtagc attagcttta ggaatagttc
      241 ctgttggatt ttcaaaagca aattacggtg taagtgctga taaaggagtt ttaccatgga
      301 cagaagaaaa aatcaaagaa ctaaatggta aagctaacct atttgacgat ttggatggac
      361 ttaactttga agcaatatca aattctaaac cagatgttat cttagcaggt tattctggta
      421 taactaaaga agattatgac actctatcaa aaattgctcc tgtagcagca tacaaatcta
      481 aaccttggca aactttatgg agagatatga ttaaaattga ttcaaaagcc ttaggtatgg
      541 aaaaagaagg tgatgagtta atcaaaaata ctgaagctcg tatatccaaa gaattagaaa
      601 aacatccaga aatcaaagga aaaatcaaag gaaaaaaagt attatttact atgattaatg
      661 ctgcagatac atcaaaattc tggatttata ctagcaaaga tccaagagca aattatttaa
      721 cagatttagg tctagttttc cctgaatcat taaaagaatt tgagagtgaa gatagttttg
      781 caaaggaaat ttctgcagaa gaagcaaata agataaatga tgctgatgta atcataactt
      841 atggtgatga taaaactctt gaagctttac aaaaagatcc tcttttaggt aaaataaatg
      901 caattaaaaa tggtgccgtt gctgtaattc cagataatac accgttagca gcctcatgca
      961 ctccaacacc actttcaata aactatacta ttgaagaata tctaaatctt ttaggaaatg
     1021 catgcaaaaa tgcgaaataa aaaacaaata aacctaggca taa
//
LOCUS       DAVLXF010000185         1050 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVLXF010000185 DAVLXF010000000
VERSION     DAVLXF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.185"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..641
                     /locus_tag="VYO12_002141"
     CDS             <1..641
                     /locus_tag="VYO12_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1588653.1"
                     /translation="TLNIKIVVKDFARNTTVKEFILNKDTGEVSELKPHRVTVTIQNG
                     KEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVINLSKDTFIKPVF
                     KKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHSQKSDSTKDVTAT
                     VLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD"
BASE COUNT          412 a          132 c          200 g          306 t
ORIGIN      
        1 agacactaaa cattaagatt gttgtaaaag attttgcaag aaatacaacc gtaaaagaat
       61 tcattttaaa taaagatacg ggagaggtaa gtgaattaaa acctcatagg gtaactgtga
      121 ccattcaaaa tggaaaagaa atgagttcaa cgatagtgtc ggaagaagat tttattttac
      181 ctgtttataa gggtgaatta gaaaaaggat accaatttga tggttgggaa atttctggtt
      241 tcgaaggtaa aaaagacgct ggctatgtta ttaatctatc aaaagatacc tttataaaac
      301 ctgtattcaa gaaaatagag gagaaaaagg aggaagagaa taaacctact tttgatgtat
      361 cgaaaaagaa agataaccca caagtaaatc atagtcaatt aaatgaaagt cacagaaaag
      421 aggatttaca aagagaagat cattcacaaa aatctgattc aactaaggat gttacagcta
      481 cagttcttga taaaaacaat atcagtagta aatcaactac taacaatcct aataagttgc
      541 caaaaactgg aacagcaagc ggagcccaga cactattagc tgccggaata atgtttatag
      601 taggaatttt tcttggattg aagaaaaaaa atcaagatta agacaaaagc tatagaaaaa
      661 aatggtttat gtactgagat tagatagtga ggtgatgaca tagttttgtg aaaacagcca
      721 tttataactc aattatttag tttacttcac tattgatact atttggagtt attaatggac
      781 ttagtttaga taactaatga attgattgaa agggttagta ttgacaatat tggtcatatt
      841 gattagaaaa tagagtctat caaaatttaa aggctaatag aggtgatgag acaatttcaa
      901 ggaattaagt agcgtttggt tgcttaattc ctttttattt ggaaggaagt ttttcgtaac
      961 cgcccaataa cgaagtctat tggacggtta cgatatgatc actgacttcg tcagtcttat
     1021 ctacaacctc aaaactgtgt tttgagcaac
//
LOCUS       DAVLXF010000186          983 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVLXF010000186 DAVLXF010000000
VERSION     DAVLXF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.186"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            95..844
                     /locus_tag="VYO12_002142"
     CDS             95..844
                     /locus_tag="VYO12_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1588654.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFQYVSDSSMVGYMGKTKELKMYQEELDEKNYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            857..>983
                     /locus_tag="VYO12_002143"
     CDS             857..>983
                     /locus_tag="VYO12_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV1588655.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGI"
BASE COUNT          344 a          108 c          192 g          339 t
ORIGIN      
        1 tattcattat taagaataag gtgaattttt ttgatgatgt ggaatttaat cagacactaa
       61 gtgagttttt agataaattg tagagggaat ggttatgagt aacaatacta agtatatctt
      121 tttatctcca catttagatg acgcaatttt ttcctgtggt gattatattt caaaattaac
      181 ttcagaagga gaaattgtac ttgttattac tatattttct ggttaccctt tgagccaaca
      241 gctacagccc tctgctaagc aatttcataa gttgtgtaac cttgggaaat atccgattga
      301 agaaaggaag aaagaagaca ggttagcttg tgaacgtcta caatgtgatt ttagacactt
      361 atcgtactat gaatgtctct ataggaaaga tagaaatggc aattttctat accgtcatat
      421 ctatagtgaa ttaaaaaatg aggacacatt aaagaatgat atcataaaag agctgttaat
      481 gcatttagac gacaaatgtg tcgtatattg tccgctttca cttggtgacc atgttgatca
      541 tgtttttgtg aatagtattg gaagggcgct tgaatttatg cgttataaag taatatatta
      601 tgaggacttc caatatgtaa gtgatagtag tatggttggt tatatgggaa aaactaaaga
      661 attaaaaatg tatcaagagg aacttgatga aaaaaattat attgatagaa tatcatcaat
      721 attgtgctat aaatctcaga ttttgataat ctggaaatca gttgaaaaac ttttaaacaa
      781 tattaaagag ttgtatttaa ggaatggggc agcttattct attagatttt ggattaagaa
      841 ataaggaagt agttttatga atatagcagt aattggtttg gggcatgttg ggctggccta
      901 cgcgttacta tttgcatcta aatataaagt tgttgcatat gatatagatt ctgtaaaaat
      961 aaataattta aaaaagggca ttc
//
LOCUS       DAVLXF010000187          976 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVLXF010000187 DAVLXF010000000
VERSION     DAVLXF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.187"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>976
                     /locus_tag="VYO12_002144"
     CDS             <1..>976
                     /locus_tag="VYO12_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1588656.1"
                     /translation="LDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGGESYKLYSKSN
                     SKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIISDIQDRLGHGT
                     AVVAQIVGDNNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDGNDIINLSTGQ
                     YLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSNQSDLLKLISS
                     RKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPAGSTLSLSELG
                     LNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYKL"
BASE COUNT          360 a          119 c          196 g          301 t
ORIGIN      
        1 ctagataata gaggaggaaa tatagagcat gacaaagata acttagaatc gtcgattgta
       61 agaaaatatg aatgggatat agataaagtt actggtggag gcgaaagtta taaattatat
      121 tctaaaagta attctaaagt ttcaattgct attttagatt caggagtcga tttacaaaat
      181 actggattac tgaaaaatct ttcaaatcac tcaaaaaact atgtccccaa taaaggatat
      241 ttaggaaaag aggagggaga ggaaggaata atatcagata ttcaagatag attaggtcat
      301 ggtacggctg ttgtagctca aattgtaggg gataacaata ttaatggagt aaatcctcac
      361 gttaatatta acgtctatag aatatttggt aagtcgtcag ctagtccaga ttggattgta
      421 aaagcaattt ttgatgctgt agatgatggc aatgatatta tcaatcttag tactggacaa
      481 tatttaatga ttgatggaga atatgaggac ggaacaaatg attttgaaac atttttgaag
      541 tataaaaagg ctattgatta cgcgaatcaa aaaggagtaa ttatagtagc tgcattaggg
      601 aatgactccc taaatgtatc aaatcagtca gatttattga aacttattag ttcacgcaaa
      661 aaagtaagaa aaccaggatt agtagttgat gttccaagtt atttctcatc tacaatttcg
      721 gtcggaggca tagatcgctt aggtaattta tcagatttta gcaataaagg ggattctgat
      781 gcaatatatg cgcctgcagg ctcaacatta tctctttcag aattaggact taataacttt
      841 attaatgcag aaaaatataa agaagattgg attttttcgg caacactagg aggatatacg
      901 tatctttatg gaaactcatt tgctgctcct aaagtttctg gtgcgattgc aatgattatt
      961 gataaataca aattaa
//
LOCUS       DAVLXF010000188          975 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVLXF010000188 DAVLXF010000000
VERSION     DAVLXF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.188"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(55..930)
                     /locus_tag="VYO12_002145"
     CDS             complement(55..930)
                     /locus_tag="VYO12_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1588657.1"
                     /translation="MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNP
                     IADPGILGINTGASLSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGL
                     TPIKLALSGTCVSMALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFII
                     LGHLIAIFISSDLNALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFV
                     GLIVPHFCGLFISKDIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGG
                     PVLIYVTMKNRGVNN"
BASE COUNT          343 a          184 c          137 g          311 t
ORIGIN      
        1 aaaatagtgt tattctattt tttctttttt gaatacctat aattaaattt tgcattagtt
       61 attaacccct ctatttttca tagttacata aataagtact ggacccccga ttattgcagt
      121 aattatccct acttcaattt cacctggttt acctaacata cggccgatta tatcacatat
      181 aagcaagagc tctgcaccta taaaagatga agaaatggtc attgtgcgta tatctttgct
      241 tataaataag ccacaaaagt gaggaactat aagacctacg aagccaatag gtccaccaat
      301 tgcagtaata cttgaacata aaagcacact tgcaattatt gcaagtgatc ttatcctatt
      361 aacattaact ccaagaccaa cagccatttc atcacccata gctaaagcgt ttaaatctga
      421 tgaaataaat atagctatca agtgacctaa aattataaaa ggtagtagtg tagatataga
      481 agataatgta gctgctccaa ggctacctat ttgccaaaat ctaaatttgt ctaagacgtt
      541 attattcggt aaaattaaaa aacttacaaa actgcttaaa gccatactaa cacaagttcc
      601 tgataaggca agttttatag gggtaaggcc tgcttttccg cttacagcaa tcgcgtatac
      661 aaaaattgca cttactaagc caccaatgat tgcaaaactt atatggctta tgcttgatga
      721 aattcctaaa aaagaaagac caattactac actaagactt gctcctgtgt ttataccgag
      781 tatacctgga tcagctattg ggtttctagt aactgattgc atcaatacac cgcttatggc
      841 aagactagaa cctgctaaaa ttgcaaaaat agttctaggt attcttttat atataattga
      901 tttaataaaa tcatcatttg tattacccat tccaaaagct gctaaaaaat ctctgatatt
      961 aatttctttt gttcc
//
LOCUS       DAVLXF010000189          970 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVLXF010000189 DAVLXF010000000
VERSION     DAVLXF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.189"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..>970)
                     /locus_tag="VYO12_002146"
     CDS             complement(<1..>970)
                     /locus_tag="VYO12_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1588658.1"
                     /translation="ELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELA
                     SRIASVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDYVRISFD
                     VTFPVALVNDIPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSEDYQTNNV
                     FIRFGTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQRADSLE
                     AGVSRLTEGLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIR
                     QEILNATKDKASKSELTQTAEELASKIASVQASGRNLFLNSLFKQDI"
BASE COUNT          251 a          237 c          206 g          276 t
ORIGIN      
        1 aatatcctgc ttgaatagtg agttcaagaa tagatttcga ccggatgcct gcacactcgc
       61 tatcttacta gccagctcct cagctgtctg cgtgagttct gacttgctgg ctttatcctt
      121 ggttgcgttc aggatttcct gacggataga gccagctcgc acctcaaatt cagcctgact
      181 caacttctga tttagcttgt tctgcgtgtc tgtctcaaga ctcttcacag attgccggat
      241 attttcagca gtcacattga gtgagctgat atcggctttg gttctaaggc cttcagtcag
      301 acggcttaca ccagcttcga gtgaatcagc acgctgttta aagttggatt cgactactga
      361 gacacggtct tcttggtctt catacgctgg ttgataggct ggaaaataat taccaaccga
      421 taacatagcg ttctcaatga cgacctgcag accagcagga aatccataat tagtaccaaa
      481 acgaatgaac acattattag tctgatagtc ctcagaagaa ctagacaagt caatcgtaaa
      541 ctcaaaatgt tggcgttcga cagttccacc tttaaaaatt aagtttctgt aggcatacca
      601 tggatgagca ctaaaatgca ccatagcagg tatgtcattt actagggcga cagggaaagt
      661 cacatcaaaa gatatgcgaa cataatcacg cttgaacctg tcactgttct tccagaaatc
      721 aggaactatg aatgttcgat agtcgtatac cgcttgacct cctgttgtga acgttcttga
      781 acgtgaattc ctgaagtaat tccgtgaact acctgcctgc acactcgcaa tccgactagc
      841 cagctcctca gctgtttgcg tgagttctga cttgctggct ttaccattgg ccaagttggt
      901 cagttctgac agtctacgag tcgtcgtctc ctcatacgtc gcttgcgctg acttcacgcc
      961 agccagttca
//
LOCUS       DAVLXF010000190          957 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVLXF010000190 DAVLXF010000000
VERSION     DAVLXF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.190"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..745)
                     /locus_tag="VYO12_002147"
     CDS             complement(<1..745)
                     /locus_tag="VYO12_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588659.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPFSVFTFMFSYILLFVQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYK"
BASE COUNT          368 a          163 c          124 g          302 t
ORIGIN      
        1 ctttataaag caaaggtaaa actttataaa aaatatctaa gtcaacctca ctctgattct
       61 gaagcaagta ggagtcaagc aaattttcaa aaatagctaa caatatgatt ggtgtaaagt
      121 ttttgtattc acatgtcaag tattctgagt atcgtgcaga tgatatttct tttatataac
      181 tttttgaaag caagattttt tcaaattcat catctattat taatcttcta tttccaatcc
      241 aagcggtatt accacccaaa taagattgta caaatagcaa tatataagaa aacatgaacg
      301 taaatactga aaaaggtaga attctatgcg taaaaaaata gacgataatg gtagccagga
      361 gtaaagatat ctctcgtgac aatcgaattc ttttcagcat cttttttaac agtccgtaag
      421 gttgtccttg gcaatattct tgaactaaat taatcggaat gacatctctc accatttctg
      481 gaaagtagag taagtttata ggttgaaatc tccagctccc atcataagta aatggatata
      541 gaaccaaata cttaaaagat ttttttctta taaacttaat tatcacaata tgaatgactg
      601 ttgcaatgaa gtatattaaa tataaaagta aaaccgccat caatatactt acaaaaatcc
      661 ccttggaatc aatcaatgga aaataaaata ttgcaactag aagcgaataa agtcccgcta
      721 ataaatattt ctgatcatct ttcattcaaa acaccatatt atttactaag aatgcatcaa
      781 tacaatagag caactctgta agtcaatatt tgataaagca aagctttaaa cccttcccca
      841 gacgttagct tttattcaac ccactacagt tgacaaggag cccaaattat tgataaaaac
      901 tttagtcatc atcgtctcta taaaaatatt ttctatacac tcttgataat accaaaa
//
LOCUS       DAVLXF010000191          914 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVLXF010000191 DAVLXF010000000
VERSION     DAVLXF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.191"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(262..432)
                     /locus_tag="VYO12_002148"
     CDS             complement(262..432)
                     /locus_tag="VYO12_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588660.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(445..891)
                     /locus_tag="VYO12_002149"
     CDS             complement(445..891)
                     /locus_tag="VYO12_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1588661.1"
                     /translation="MNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMPMKFNTII
                     SEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIKSQGCTQII
                     VAHRLSTIKDADIIFVMKGGKIVESGNHKYLMELGGEYYSLYTKRK"
BASE COUNT          292 a          179 c          118 g          325 t
ORIGIN      
        1 actaggaagc gagccgcagg ctgtacttga gtacggcaag gcaaagctga cgtggtttga
       61 agagattttc aaagagtatt aaacaaaaag aaaaaccgct actcctaaga atagcgattt
      121 aatcatgttt ttaagctact actgtagttg ctctattatt taagtgttat gaataacttc
      181 aatatcttta aagctttttg ttagatttat aatttcaact atagccatct ctactcctta
      241 gtttacagca aaaaattatg cttaattctt attgtttttg taaacaatca gcgaacccaa
      301 tactattcca ttcaacaagc ctaaagaaat accatattca actggaaaat ctaaaaaaat
      361 tgcaaacact attccaatca tcacccctag tgaggctgtt gttaaaatta catattcatt
      421 ttctttcttc attttcttta cacctcattt cctttttgta tataagctgt agtactctcc
      481 accaagctcc attaagtact tatgatttcc tgactcaaca atcttaccac ctttcattac
      541 aaaaataata tccgcatcct taatcgttga caatctatga gctacaatta tttgagtaca
      601 gccctgactt tttatatact ttgttattct ttcctcatta atagtgtcta aagcactagt
      661 tgcttcatct aaaattacaa tactaggatt atttattaat gcacgtgcca gtgctatccg
      721 ttgtctttga ccacctgaaa tatttgaacc catctctgag atgatagtat taaatttcat
      781 cggcatagcc atgatttcat catagatttg aactgcttta caaacttcct ctatcttttg
      841 tgaagtaact tcgttcttta aagttatatt atcaagaata cttcggttca ataaaaatga
      901 atcctgtgga acta
//
LOCUS       DAVLXF010000192          846 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVLXF010000192 DAVLXF010000000
VERSION     DAVLXF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.192"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..776
                     /locus_tag="VYO12_002150"
                     /pseudo
     CDS             1..776
                     /locus_tag="VYO12_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            769..>846
                     /locus_tag="VYO12_002151"
     CDS             769..>846
                     /locus_tag="VYO12_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1588662.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRA"
BASE COUNT          308 a           76 c          181 g          281 t
ORIGIN      
        1 gttaagacag tgataacatg ccatggatgg gttgaatata acttaaaaaa gaaattcttg
       61 acttattttg atttttggac atattctatg ggagatgctt ttatttgtgt atctgaaact
      121 atgaaaaaaa gattggagag tataataaaa aaataagaag atagttgcaa taaataacgg
      181 aattaatgta tcaaatagcg atttagatgt agtaggtgtt caagacttta aaaaagaatt
      241 ttgtattcca aataacaaaa aaatcatttg ttatgttgga aggttggatc cagaaaaaag
      301 gccggataga ttccttgaat ttgcagaaaa attattttta gtcagagaag atgtgatttt
      361 tattatggct ggaaatggta gtatgtgggc tgctttgaaa gaaaaaattt gccatttgaa
      421 atgtagagat aatttcaggt tacttggtga aatctatcca gctactatag tttaccaaat
      481 atcggattta ttgtatattc cttctgatac agaaggaata cctatgtgtg ttttagagtc
      541 aatgtcacaa ggaactccgg ttttagctag taatgttggc gggttaagtg aaattattga
      601 acatagggtt gatggatttt tatttgagaa ggaagatgtt gagggagtgt gtgcttgtgc
      661 taatttttta ctcaatgatt ctgagtattt gaaatatata ggtgagaata gtaaatcaaa
      721 aataagaaaa catgagagta tatgatgaat tattagagaa gagtagtcat ggataggaat
      781 atagatcaag aattagtaag tattataata ccaacacata ataggtatga atctctgatt
      841 agagct
//
LOCUS       DAVLXF010000193          839 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVLXF010000193 DAVLXF010000000
VERSION     DAVLXF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.193"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            62..766
                     /locus_tag="VYO12_002152"
     CDS             62..766
                     /locus_tag="VYO12_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEV1588663.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
BASE COUNT          304 a           98 c          154 g          283 t
ORIGIN      
        1 tgtttttgta ccatactatc ttgaaaaatc tactaaattc aaattgagag gatgaattaa
       61 aatgaataaa atatttatat atgcaggagt gcgaaatcat aactctaaga ctttagagta
      121 tactaagaga ctttcaagta tcatttctag tagaaataat gttgatatat ctttcaggac
      181 acctttcaat tcagagctag aaatatctaa ttctgattca gaagagttgt ttaagaaagg
      241 aattgatagg caaagtaatg ctgatgatgg aggtgttata aaaaaagagt tattagagtc
      301 tgatattata attatctctt ctcctgtata tttacaaaac gtatctgtgg acacaaaaaa
      361 ctttattgaa aggattgggg ggtggagcca tctttttcga ttagctggaa agtttgttgt
      421 aacattagat gttgctgaga gtaacggctc tgataatgtg tcagaatatc ttagagatat
      481 attttcatat atgggaggac agatattaca ccaagtttca attaccaatt cactcaaaga
      541 tattgcagag gctcagttga tggaggcaac ttataaaatt gaagatgtac ttgaagggaa
      601 aattaagtac aaaacaacgg attatcaaga aagagcttac cagacattaa aattaatttt
      661 agaaaattat gattccgaac attttgagaa gatgtattgg gagaaaaaac gcctttttga
      721 agctaatagt ttagaggaat ggtattatgt tgaaaatatt aaataactct gtcatatatt
      781 tatgtttgat tccgttattg ttcctattat tgataatttt tccaaatgat tcattaatc
//
LOCUS       DAVLXF010000194          822 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVLXF010000194 DAVLXF010000000
VERSION     DAVLXF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.194"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            29..>822
                     /locus_tag="VYO12_002153"
     CDS             29..>822
                     /locus_tag="VYO12_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588664.1"
                     /translation="MLPLFVLWLELHLLVSNLQLNLEIPLDFVVSTSLAFCSLILSKI
                     VLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSPDDLRSRIIWS
                     FILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQNKWEKEDSFES
                     DLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCT
                     GLVELLLYEMSISNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQ"
BASE COUNT          261 a          119 c          136 g          306 t
ORIGIN      
        1 gatacgtaaa ctaatcattt ttttattaat gctccctctt tttgtcttgt ggctagagtt
       61 gcatttgtta gtgagtaact tgcaattgaa tctagaaatt ccccttgatt ttgtggttag
      121 tacgagtctt gctttctgta gtttgatttt atcaaaaata gttttagaca tactatacgc
      181 attgaaagat ttgtacaaga aagaagctct tattacgatt tttcctttta tttttatagg
      241 caggaagaaa gtaaatgtga ggttctcgcc ctatttttca ttccatcgta aaagtttgtc
      301 gcctgatgat ttaagatcta ggattatatg gagttttatt cttgaaattg ctattattct
      361 agtatttatt ttaaaaattc cttttgcaat catcatgctt actacaatat ttttctggac
      421 tataatggat atcaatcatc tcgtttttaa taagacggaa tttcttttta atcaaaataa
      481 atgggaaaaa gaagattcgt ttgaaagcga tttgacgaaa acgttaaagg ataagataca
      541 aaaatcagag ctaagttatt ctgacttaat gtctcttcag ctgtatgatg cgatgaacca
      601 atccaccttt ttaacggata gtgaactttt tgaggatatt ttgaaaaaaa tcgaagattc
      661 tcataacacc cttctgtgta caggtcttgt cgagttactg ctgtatgaaa tgtctattag
      721 taacaataac aactggtcca acaaggttga taaaattagg atacacctca tcagaatcaa
      781 tcaattagat tttttctatt atacaagttg gttaaggcaa aa
//
LOCUS       DAVLXF010000195          769 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVLXF010000195 DAVLXF010000000
VERSION     DAVLXF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.195"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(323..622)
                     /locus_tag="VYO12_002154"
                     /pseudo
     CDS             complement(323..622)
                     /locus_tag="VYO12_002154"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          240 a          131 c          138 g          260 t
ORIGIN      
        1 ggggtgcata attggggttt atataattta tttcatattt tgtaaggtca gtgtgaaaat
       61 atttaaatag acttcctgta aaaactggat ttaacgccct ccatttgtca aattaagcta
      121 gacagtctta ccaactcaga aaatacctga tgaggtattt ggtatccaaa ggatttaggt
      181 gggatgtgat ttcttgaggc tgggaaaaag tctttaaaat taaaaaatgt ataatatcag
      241 gtgttggaaa aactttgata ctatgcgttt tattgtggga atatttattt cattttctcc
      301 tgaaattgag tttttaccca gcctaaaatt cttgactgac ttcgttgaaa tctgtctgct
      361 ttggcaagtc atgacacctt aaaagtctat ttgaatgttc gttgagacac cgctgtcttg
      421 gacatcctag attcgcaaaa aagctctcac tatcgtgatg attggaaata tctttccatt
      481 ttgaaaactc ttttccacag tcaaacgtaa tagatttaaa gaggttcact agaaattgtg
      541 ccagccaccc ttggatggat tctgccatat cctttgcctt acgactcttg gtcttcaaac
      601 tgacaatcaa ttgactatac ctgttcgctc acaaatagga gagaactttt tacgaaaatc
      661 taatgaatat gccacaagaa cattataccg caacgtgtac tatttttggt tcattttact
      721 atagaaacta ttgaagcacc tgtacgaaaa tatagtagat tgaaactag
//
LOCUS       DAVLXF010000196          735 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVLXF010000196 DAVLXF010000000
VERSION     DAVLXF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.196"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            301..525
                     /gene="pneA1"
                     /locus_tag="VYO12_002155"
     CDS             301..525
                     /gene="pneA1"
                     /locus_tag="VYO12_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEV1588665.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKICISAAVSGIGGLVSYNNDCLG"
     gene            532..720
                     /gene="pneA2"
                     /locus_tag="VYO12_002156"
     CDS             532..720
                     /gene="pneA2"
                     /locus_tag="VYO12_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEV1588666.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT          259 a           93 c          152 g          231 t
ORIGIN      
        1 caaggaaaaa gaaaggggtt tcattgcatg agaaaaaaac gtggaattaa aaaattagtt
       61 acatttgcat tgctaggtgt ttttatgttt agtaatacaa ttccttacca acagtttatt
      121 cagaagaata aacaattgga gattcgagtg caatcgcaaa agaagtccaa tggtcttgat
      181 gttgggaagg cggattaaaa cccgatattt tgcacgagaa tgagagaatt ataatttatc
      241 tgaaaaaacg aaaaatatag agcaacttat ttaacttttt ataaggagga gttattactt
      301 atgaccaact ttaattcaaa cgaaaaattt tgtggaaaaa gtttaaaatc attgtctgct
      361 gatgaaatga gtttaattta tggtgcatct gatggtgcag agccacgttg gacgcctact
      421 cctattattt tgaaatcagc tgcagctagc tcgaaaatat gcattagtgc agctgtttct
      481 ggtattgggg ggctagttag ctacaataat gattgtttgg ggtaaattaa aatgaaaaat
      541 gattttgtaa tcggaaagag tttgaaagaa ttatctcttg aagaaatgca attggtttat
      601 ggtggcacag atggtgctga ccctagatca acaattatat gttctgctac attaagtttt
      661 attgcatcat atttggggtc agctcagact cgttgtggca aagacaacaa gaaaaaataa
      721 aatattcaat tttaa
//
LOCUS       DAVLXF010000197          735 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVLXF010000197 DAVLXF010000000
VERSION     DAVLXF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.197"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>735
                     /locus_tag="VYO12_002157"
     CDS             <1..>735
                     /locus_tag="VYO12_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEV1588667.1"
                     /translation="RFKCYHCSKMMVAETPLVKKNHQIPRIINQKIAQKLIEKISMTD
                     IAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSFIAQDFDNLNIIT
                     VLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQ
                     HLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRIHLTNK
                     EILDKILSYSEDLKHHYQIYQLLLFH"
BASE COUNT          226 a          157 c          118 g          234 t
ORIGIN      
        1 ccgtttcaag tgctatcact gttcaaaaat gatggttgct gagactcccc tggtaaagaa
       61 aaatcaccaa atccctcgta tcatcaacca aaaaattgct caaaagttaa ttgaaaaaat
      121 ttctatgact gatattgccc atcagctggc catttcaact tcaactgtca ttcgcaagct
      181 caatgacttt cactttgagc atgatttttc tcggcttcca aagattatgt cttgggatga
      241 gtatgccttc actaagggaa agatgagttt cattgcgcaa gattttgata atcttaatat
      301 tatcactgtt cttgaaggca gaacacaagc tgtcatccga aatcactttc ttcgctacga
      361 tagagccgtt cgttgtcaag tgaaaatcat tacgatggat atgtttagtc cttactatga
      421 cttggctaaa cagctttttc cgtgtgctaa aatcgttcta gatcgtttcc atattatcca
      481 acatctcagc cgtgccatga gtcgttttcg tgttcaaatt atgaatcagt ttgaacgaaa
      541 atctcatgaa tacaaggcta tcaagcgtta ctggaaactc atccaacagg atagtcgtaa
      601 actcagcgat aaacgttttt atcgccctac ttttcgcata cacttaacaa ataaagaaat
      661 tcttgacaag attttaagct attcagaaga cttgaaacac cactatcaga tctatcaact
      721 cttacttttt cactt
//
LOCUS       DAVLXF010000198          734 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVLXF010000198 DAVLXF010000000
VERSION     DAVLXF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.198"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>734
                     /locus_tag="VYO12_002158"
     CDS             <1..>734
                     /locus_tag="VYO12_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEV1588668.1"
                     /translation="HFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKERISKYLV
                     TNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQLASQPTLS
                     RFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEGVAYNAHY
                     RAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFRMDSGFAT
                     PKLYDLIEKTGQCYLIKLKKNTVLSRL"
BASE COUNT          200 a          164 c          136 g          234 t
ORIGIN      
        1 cacttccaaa acaagtcttt ttaccaacta tctttcgatg gaggtcattt aacccagtat
       61 ggtggtctta tcttttttca ggaacttttt tcccagttga aactaaaaga gcggatttct
      121 aagtatttag taacgaatga ccaacgccgc tactgtcgtt attcggattc agatatcctt
      181 gtccagttcc tctttcaact gttaacaggc tatggaacgg actatgcttg taaagaattg
      241 tcagctgatg cctactttcc aaaattgttg gaaggagggc agcttgcttc acagccaacc
      301 ttatcccgtt ttctttccag aactgacgag gaaacagtcc atagtttgcg atgcctcaac
      361 cttgaattgg tcgaattctt tttacagttt caccagctaa accaactcat tgtagatatc
      421 gattctaccc atttcacaac ttatggcaag caagaaggtg ttgcttataa cgcccactat
      481 cgtgctcatg gctatcatcc tctttatgct ttcgagggga agacaggtta ttgtttcaat
      541 gcccagcttc gtcctggtaa tcgttattgt tctgaagagg cagacagctt tatcacacct
      601 gttttagaac ggtttaatca acttctcttt cgaatggata gtggctttgc gaccccaaaa
      661 ttatacgatt taattgaaaa aacagggcaa tgctacctca taaaactcaa gaaaaatact
      721 gttctgagcc gtct
//
LOCUS       DAVLXF010000199          687 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVLXF010000199 DAVLXF010000000
VERSION     DAVLXF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.199"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            294..593
                     /locus_tag="VYO12_002159"
     CDS             294..593
                     /locus_tag="VYO12_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1588669.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          264 a          131 c          114 g          178 t
ORIGIN      
        1 aatagataga ggtaacatgt ttgcaaacaa agcatacgaa cctttagtaa aatcatttcc
       61 atgaaactag aatagagccc ctcttagcaa aaatcattat tttaatttat ttctaatcac
      121 tccttgacat aaataactct caccaataaa agactatgtc ttaaaaaaat ggtataataa
      181 aatcaatacc tgggcttgat ggctatgcta ctaataacaa ttaggagaga aaatcaggca
      241 cttgttaaca acaaggatta tccccttgag atgaaaggaa ctttagaaat cttatgatga
      301 acatgcaaaa catgatgcgt caagcacaaa aacttcaaaa acaaatggaa caaagccaag
      361 ctgaacttgc tgctatgcaa tttgttggca aatctgctca agatcttgtc caagcgacct
      421 taactggcga taagaaagtt gtcagcattg atttcaatcc agctgtcgtt gacccagagg
      481 accttgagac tctttctgat atgaccgttc aagccatcaa ctctgctctt gaacaaatcg
      541 atgaaactac taagaaaaaa ctgggtgctt tcgctgggaa attgccattc taaaaacaag
      601 gagctagaac aatacttgtc gataacaaag gctaagaaag gtgcaaaaat tataaaaaac
      661 gcatagtatc aagcaaccta aaacttt
//
LOCUS       DAVLXF010000200          683 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVLXF010000200 DAVLXF010000000
VERSION     DAVLXF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.200"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..683
                     /locus_tag="VYO12_002160"
                     /pseudo
     CDS             1..683
                     /locus_tag="VYO12_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; internal stop; incomplete; missing N-terminus
                     and C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
BASE COUNT          246 a           84 c          121 g          232 t
ORIGIN      
        1 tgaaattaaa aataatattg attattatat tattgcgctt ccgacagatt atgatgagaa
       61 aattggtagt tttaatacat atgaaatcga acaaacggta tcgaagattc tgagggtaaa
      121 acctaatgga aagattattt gaaagtcaac agttccgatc ggcttttcaa acaaattaaa
      181 aaggctgttt gatacaaaaa atatcatttt tgtccctgaa tttttgagag aaggttgttc
      241 tatatatgat aatttatatc caagtcgcat agttgttgga gatgagacag ttgaaggaag
      301 aaaaattgca gagttgttcc tttcgattag tactcatagt actgccaata ttaaaaatgt
      361 tatgttagtt tctcctactg aagcagaagc aattaagctt ttttctaaca cattcttagc
      421 tctccgtgtt gcttttttta atgaactaga ttcttttgct gagaggagaa gtttaaatgc
      481 tgaagttgta ataaaaggtg tttgtttaga tccaagaatt ggaaattttt ataataatcc
      541 ttcttttgga tttggaggat attgtcttcc caaagatact aaacaattaa aaaaagaatt
      601 tatagaaata aatgccccag tgatagaagc gattgatatt tcaaacacaa atagaaaaca
      661 gttcatagtt aaacaaatat tgg
//
LOCUS       DAVLXF010000201          656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVLXF010000201 DAVLXF010000000
VERSION     DAVLXF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.201"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..656
                     /locus_tag="VYO12_002161"
                     /pseudo
     CDS             1..656
                     /locus_tag="VYO12_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          230 a          117 c          132 g          177 t
ORIGIN      
        1 cgaaaaccta aattaagcct agaagacctt cttatggcca ctcttcaata tgtgcgagaa
       61 tatcgaactt atgaagaaat tgcggctgat cttggtattc acgaaagcaa cttaatccgt
      121 cggagccaat gggttgaagt aactcttgtt caaaggtggt gttgcgattt caagaactcc
      181 tctcagttct gaggacacgg taatgattga tgcgacggaa gtaaaaatca atcgacctaa
      241 aaaaagaatt agcgaattat tctggtaaaa agaaatttca cgctatgaag actcaagcga
      301 ttgtcacaag tcaagggaga attatttctt tggatatcgc tgtgaactat tgtcatgata
      361 tgaagttgtt caaaatgagt cgcagaaata tcggacaagc tggtaaaatc ttggctgaca
      421 gtgattatca agggctcatg aagatatatc ctcaagcaca aattccacgt aaatccagca
      481 aactcaagcc gctaacagtt gaagataaaa cctataacca tgcactatct aaggatagaa
      541 gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgttttca acaacctatc
      601 gaaatcatcg taaacgcttt ggattacgaa tgaatttgag tgctggtatt atcaat
//
LOCUS       DAVLXF010000202          639 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVLXF010000202 DAVLXF010000000
VERSION     DAVLXF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.202"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            387..>639
                     /locus_tag="VYO12_002162"
     CDS             387..>639
                     /locus_tag="VYO12_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV1588670.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDKSTLWNKVEMAEKNSNAQ"
BASE COUNT          279 a           98 c          108 g          154 t
ORIGIN      
        1 gattaaacat aatggaaatt ttagaagaaa gaatagatga taatgtagaa aaatcatcta
       61 aagatgaaat aacataattg tcccttggtt acaagggcgc aattatacac cctaaagggt
      121 gcttgcgtcc tgcggagtca aacccttgca gggagcaaca accattcgct ttttaaaagt
      181 caagggtaaa taaactcgct aaggctcgcc cttgactttt aaaatttgaa atgaaagtaa
      241 caattaagcc aacatactga aacaacaaaa attaattcag tagtcggctt ttttaattct
      301 atcatttcaa aacgctcatt cacaaagagg atataaaaaa actattaagc ctccacctaa
      361 aacaacaaag aaaggaagtg ataaaaatgg cagacagttt tcatttttca gtaaacataa
      421 tatcaagagg aaaaggcaaa agtgcagtag caagtgcagc atatataagt ggagaaaaaa
      481 taaaaaatga atgggacgga gtaacccatg actacacaag aaaagaaaaa atattagtaa
      541 aaaatataat attgcctgat catataccaa aagaatttaa tgataagtct accttatgga
      601 ataaagtaga aatggcagaa aaaaattcta atgcacaac
//
LOCUS       DAVLXF010000203          627 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVLXF010000203 DAVLXF010000000
VERSION     DAVLXF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.203"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            7..>627
                     /locus_tag="VYO12_002163"
     CDS             7..>627
                     /locus_tag="VYO12_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588671.1"
                     /translation="MNNVSVRYDDVIALKDITLQINKGDFIGLLGSNGAGKSTLINSI
                     VGFQEIYLGEIEYCDKDLIVSSQPFAHLGFTPQTTVIDFYTTVKDNVILGLNLAGKFG
                     KNAEKLCQIALEIVGLADKKNNLVETLSGGQLQRVQIARAIAHNPDFYILDEPTVGLD
                     TESAEKFLMYLKDKSLEGKTIIISSHDINLLEKFCKKILFLQNGSIS"
BASE COUNT          220 a           77 c          117 g          213 t
ORIGIN      
        1 ttgaaaatga ataatgtaag tgttcgttat gatgacgtaa tagctttaaa agatataact
       61 ttacaaataa ataagggaga tttcattggc ttattaggtt caaatggtgc aggtaaatct
      121 acgttaatta attctattgt aggttttcaa gagatttatt taggagaaat agagtattgt
      181 gataaagatt tgatagttag ttctcaacct tttgctcatt taggctttac tcctcaaacc
      241 acagtaattg atttttatac tactgtgaag gacaatgtaa tattggggct gaaccttgct
      301 ggaaagtttg ggaaaaatgc tgagaagttg tgtcaaatag ccttagaaat tgttgggtta
      361 gctgataaaa aaaataattt ggtagaaaca ttgtcaggtg gacaactgca acgcgtccag
      421 attgctagag caatagctca taatccagat ttttatattt tagatgaacc taccgttggt
      481 ttagatactg aatctgccga aaaattttta atgtatttaa aagataagag tttggaagga
      541 aaaactatta tcatatcttc acatgacata aatctactcg aaaagttttg taaaaaaata
      601 ctttttttac aaaatggctc catatca
//
LOCUS       DAVLXF010000204          610 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVLXF010000204 DAVLXF010000000
VERSION     DAVLXF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.204"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>610
                     /locus_tag="VYO12_002164"
     CDS             <1..>610
                     /locus_tag="VYO12_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1588672.1"
                     /translation="LSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYG
                     IIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDE
                     VFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFV
                     FFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNE"
BASE COUNT          239 a           71 c          114 g          186 t
ORIGIN      
        1 ttaagtaaat taaaagttcc ttatttaaaa gaaggtggag agtatcagat aaaaaatctt
       61 agctacaaat ttactgatga tgagtgttta agcttaaaag atatttcgtt caagcttggg
      121 aaaatttatg gcataatagg atccaacgga cgaggaaaat caacgctttt aagatgttta
      181 ataggtcttg agaaaaaatc aaaagaagaa atttatttta agggagagaa gctatctaaa
      241 aaagaaagac tcaaaaactc ttcacttgtt atgcaagatg taaatcatca attattcaca
      301 gatgaagtat tcaacgagct tagattagga gtaaagaatt ttgatgaaga aaaggcgaaa
      361 atcatcttaa aagatttagg cctggacgaa tttattgaaa ggcatccgat gagtttatca
      421 ggagggcaaa agcaaaggct tgcaatagca tctgttatgt gcaagaattc tccatttgtc
      481 ttttttgacg aaccttcaag tggtatggat tattccaata tgataaaaat atctgaactg
      541 attaataagt ataaaaccat ggataaaata atttttattg tttcccatga tatagaattt
      601 ttaaatgaag
//
LOCUS       DAVLXF010000205          593 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVLXF010000205 DAVLXF010000000
VERSION     DAVLXF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.205"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(148..522)
                     /locus_tag="VYO12_002165"
     CDS             complement(148..522)
                     /locus_tag="VYO12_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV1588673.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          203 a          130 c           74 g          186 t
ORIGIN      
        1 aatcaaaaaa acgacattca tccaaataat caactttttt atttcttcgc taacaaaatc
       61 ataggctaaa aaaattatgg atgatataaa tagacataca aaaaaaataa taacaaaaat
      121 tttttccatt ttaataccca tatctagtta ccacaaggcg tttaaatttg ggtctctcct
      181 atttacatca ttgttggccc agattgttgt aatagcatcg ccaattgaca tccaagctgc
      241 aaatccatta atagcagaca gtgcaaatcc tgccactcta tttgcaactc gtaccccaac
      301 atatcttgca gctgctccag caattgcgct tgtaactcgc cgcaaaccat aattacgaat
      361 ataatttcta actagactga ttgctcctcc acctaaaaca ccattaaaag ctatattaat
      421 ggctgttgca acaggccatc tcagaataga aaatccccca tcaagataca tcatctcttc
      481 ttgctcaaga gcaacataat tatttggtaa tacgagttcc atttattttc tcctttttta
      541 ttaagcatag ttaggatcgt ttcctaacaa gtgatagacg actgtgtttt tat
//
LOCUS       DAVLXF010000206          587 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVLXF010000206 DAVLXF010000000
VERSION     DAVLXF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.206"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
BASE COUNT          222 a          107 c           66 g          192 t
ORIGIN      
        1 ttagatttat tttagtatat ccaatcacta gtccaataat tggactagca gacatgctta
       61 gatataataa aaataacaaa aaatgaattt caccaattcc tgcaaaaaaa tgtttcatag
      121 ctatagtttt acgcccataa atacccatca aatttaccgt ctacgatatc taaaccatat
      181 gcaatggctc ctcctaatga gaagacttca gcaacagtag ccgcaatatt aagagcatta
      241 gtaatagttg ccatactaac tccaccaata tatcttgtta tagcacttag ttacctagat
      301 aagatatcct ttagcttgct tccaaaattt aagtctacac ctataatatg agttcatatt
      361 agtactgcta aatcctatct atagtaatgt caaacaccta aataaagtca tctctccaat
      421 tcattcaaga aatgaagaaa ggcaaaaaat ctttaaaata agaaaaatgc atattatcag
      481 gtgttcaaaa atattgatga tatacgttat atagatagaa agatttactc aatctcattt
      541 tcaaattgag ttttttccca gcctcatttt taatttatat cttcaat
//
LOCUS       DAVLXF010000207          564 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVLXF010000207 DAVLXF010000000
VERSION     DAVLXF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.207"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..488)
                     /locus_tag="VYO12_002166"
     CDS             complement(<1..488)
                     /locus_tag="VYO12_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1588674.1"
                     /translation="MGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVLKNL
                     ALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRD
                     IFITKQEFGDIVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKEMLKDIYLNR
                     SK"
BASE COUNT          192 a          102 c           64 g          206 t
ORIGIN      
        1 tattttgaac gatttagata aatatcttta agcatctctt ttttagtttc aaaatcaact
       61 actttaatag agttttcgtt aaaaaattta aaaagctcac tactttgaat attcccagac
      121 atagtaattc tcagtccctc ttcctgaaca atatccccaa attcttgttt ggtaatgaaa
      181 atgtctctat ctgtagctga tgaaaaagct atctcaaaca agaaattatg actatctttt
      241 cctacttttt gaaatgtcaa aagcccgttc tccaagaaaa gaaccctctc acaaatttct
      301 tcaatatctt ctaatttatg actcgatatt aaaattccca cattttcatg taaagctaaa
      361 ttttttagaa ccgataaaac tatttgtgat gattcaatat ctaaaccatt agtcggttca
      421 tctaaaatca atatatcagg ttccgtaacg agagttagaa gcaaagctaa tttttgtttt
      481 gtacccaaag aataggtctt tactttttta ttaatagact gagtcaaatc taactcttgg
      541 atcaaacttc taaatctttc ttga
//
LOCUS       DAVLXF010000208          562 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVLXF010000208 DAVLXF010000000
VERSION     DAVLXF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.208"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(153..485)
                     /locus_tag="VYO12_002167"
     CDS             complement(153..485)
                     /locus_tag="VYO12_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1588675.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
BASE COUNT          191 a          122 c           68 g          181 t
ORIGIN      
        1 cacagaatat agaggtagtg atactattga gtagataaaa gctaaaaaaa taagcaaaaa
       61 atactccgtg tcatttataa tcgccctgaa tgataaaaat atatacaccc aacatacgat
      121 aatatgtatt tttcgaatct tatctacatt attcatctta ctgtcgcaga tataaatcca
      181 tcccaaggat aagggttccc gctaatatca caacctctat taagagcacc atatccaata
      241 cagtaagcta tttttcctgc tgctgcacca ataagccagc ctccaagacc tccaacctta
      301 atatagttta ttagtttttt ggttaaaact gcagctccag ctaatgctat aaaagttcct
      361 gaactcatag ctaaagctac gcaaattcct tgacaagcac gcttgcttaa ataagctccc
      421 ccatcaagat acatcatttc ttcttcatca atcacaacat aattatttgg taatacaagt
      481 tccatttgtt ttctcctcct tagttgaaat tgtttcattt gttttacaaa taggacgaac
      541 ttaaactatc tttttattac tc
//
LOCUS       DAVLXF010000209          545 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVLXF010000209 DAVLXF010000000
VERSION     DAVLXF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.209"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>545
                     /locus_tag="VYO12_002168"
     CDS             <1..>545
                     /locus_tag="VYO12_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1588676.1"
                     /translation="VFIDATGNATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHI
                     EKTKQWNYIEGYNQWLEEVLANRKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNY
                     IKERWEKFRFIYTLEVSLFRNELKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQ
                     NVALINVAKAFGFNPKYMEDE"
BASE COUNT          203 a           62 c          113 g          167 t
ORIGIN      
        1 aagtttttat tgatgctaca gggaatgcaa caattgcttc tgctagtgga gctgatgttt
       61 atgaaaaaaa ttcttcttac ggttgtttaa tgagagttgg aggagttgat atctcaaaga
      121 ctatagaaca tattgaaaaa acaaagcagt ggaactatat agaaggatat aatcagtggt
      181 tagaagaagt attagcaaat cgaaagtaca gagccactgg agcagtttta aaagatccag
      241 tttcttatga tcatgcccct atgctttcaa aagacgacca ttttatgaat gatgaaaaat
      301 ggaattacat taaagaaaga tgggaaaaat ttagatttat ttacacttta gaagtatcac
      361 tatttaggaa tgaattaaaa aaagctgtag aatctgggga ttttaatttt gaaatttatc
      421 atgatgataa atctggagtc actatgaatg gcgacggtat aagctatggt tcatggggtc
      481 aaaatgtagc attaattaat gttgcaaaag catttggatt caatcccaaa tatatggaag
      541 atgaa
//
LOCUS       DAVLXF010000210          535 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVLXF010000210 DAVLXF010000000
VERSION     DAVLXF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.210"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            177..>535
                     /locus_tag="VYO12_002169"
     CDS             177..>535
                     /locus_tag="VYO12_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1588677.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDI"
BASE COUNT          177 a           61 c          125 g          172 t
ORIGIN      
        1 caaagagata tgagtgagtg ggattcattt atcaatgaag ctaaatctaa agacttggat
       61 aaagttacaa aagtatataa tgatgcctac aaacgtgtca aagatagaat gaaatagact
      121 aaatggtagc tctctggttt ttcagagagc tactactgcc tgagggggag taagctttgt
      181 atgatgtagt aattataggt gcaggaagtt cgggcgcatt ggctgcaatt gcttcggggc
      241 gtctagggtt aaaaacttgt ctaatagaga agggaagtag aattggtgga gttcctatta
      301 atacactaat ggggtcattt gctaatctat attttgatga ttctggtaga gtaaatgcaa
      361 gtaaaattgt tggggaatta atagaacgta ttatcaaaaa aggtggtact gtctttaact
      421 ctatggaaga attgatgaat gtaaatgatt tttatcagat tacaattccc tatcaaccag
      481 aagtttatga atcggtttta actgatatgt tgattgagtc taaagttgat atttt
//
LOCUS       DAVLXF010000211          523 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVLXF010000211 DAVLXF010000000
VERSION     DAVLXF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.211"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..>523)
                     /locus_tag="VYO12_002170"
     CDS             complement(<1..>523)
                     /locus_tag="VYO12_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1588678.1"
                     /translation="ILPHSAYSETLYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTN
                     MTSGTSQDQFQLYRGRGQAENFIKEMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYL
                     FLKHLAGGDFQTLTIKRFRHLFLHVVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIR
                     KVNLNLPVPYEPPG"
BASE COUNT          153 a          102 c          120 g          148 t
ORIGIN      
        1 ttccaggtgg ttcataagga acaggaagat tcaggttgac ttttctaatc ctagaataaa
       61 gtgctgaaaa caattcggaa taggcataga gactagacaa tttgaggagc tgcttgcgtc
      121 ctgttcgaac acattttcct accacgtgaa gaaaaagatg gcggaagcgt ttgattgtta
      181 aagtttggaa gtcacctcca gctagatgtt tgagaaaaag atagagattg taggcgatac
      241 agctcatcat catacgaact tcgtttttga ttaaggttga actatccgtt ttatcgccaa
      301 aaaatccctc cttcatctcc ttgatgaaat tctcggcttg accacgtcca cgataaagct
      361 gaaactggtc ttggcttgtt ccactcgtca tatttgtaac gagagaaata acatcgtaga
      421 acaagtttcc ttcttttcgt tcagagaact ggcagacacg acgcttgtgc gaccaagatc
      481 ctgcttgata gagagtttct gagtaggcgg agtggggcaa gat
//
LOCUS       DAVLXF010000212          500 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVLXF010000212 DAVLXF010000000
VERSION     DAVLXF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.212"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            8..>500
                     /locus_tag="VYO12_002171"
     CDS             8..>500
                     /locus_tag="VYO12_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1588679.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNN"
BASE COUNT          185 a           97 c           80 g          138 t
ORIGIN      
        1 gaaacaaatg attacaaaac aatcagcctt tttacaaaat agagccacta tactggagtt
       61 tctatatcga aatccagcaa catcccgtac agatattgtt aatgaaacag gacttactcc
      121 agcaacaacg actaacatca taaaagagct aagcgaacag tccctcatat acgaaactgg
      181 tgatgaattt agcgagtttt ccggatctgg aagacgtaga aaaactattt ctatcacaga
      241 taatattccg tatgttgttg gaggtattga aataaacgtc ctaggtatct ttctcagtct
      301 atgtgactta caagggaaaa ctcttttcga gacagaaatt ttgaatgaag attatcctat
      361 ttcagaaatc aattccacca ttaccaatat gataaaaaca gctatagagt acgtcccttt
      421 ggaaacaaaa ttacttggat ttggcttatc aatacctgga cattataaca aagactccgg
      481 aagtatcatt acaaacaacc
//
LOCUS       DAVLXF010000213          499 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVLXF010000213 DAVLXF010000000
VERSION     DAVLXF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.213"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>499
                     /locus_tag="VYO12_002172"
     CDS             <1..>499
                     /locus_tag="VYO12_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1588680.1"
                     /translation="EIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIK
                     KDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMP
                     YNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPN
                     ERPHS"
BASE COUNT          161 a          104 c          113 g          121 t
ORIGIN      
        1 atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt tatatctttg
       61 atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat atgacccata
      121 gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc caggcttatg
      181 ctaaagagaa aggtttgacc cctccttcga cagaccatca ggattcagga aatactgagg
      241 caaaaggagc agaagctatc tacaaccgcg tgaaagcagc taagaaggtg ccacttgatc
      301 gtatgcctta caatcttcaa tatactgtag aagtcaaaaa cggtagttta atcatacctc
      361 attatgacca ttaccataac atcaaatttg agtggtttga cgaaggcctt tatgaggcac
      421 ctaaggggta tactcttgag gatcttttgg cgactgtcaa gtactatgtc gaacatccaa
      481 acgaacgtcc gcattcaga
//
LOCUS       DAVLXF010000214          498 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVLXF010000214 DAVLXF010000000
VERSION     DAVLXF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.214"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            303..>498
                     /locus_tag="VYO12_002173"
     CDS             303..>498
                     /locus_tag="VYO12_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588681.1"
                     /translation="MDTYQRVNRLPAKQNYEASKQLTDARFKRLVGVQRTTFEEMLAV
                     LKTAYQLKHAKGGRKPKLSLE"
BASE COUNT          209 a           85 c           81 g          123 t
ORIGIN      
        1 aaattaaccc actttaattt cttttttaac ccgtgttaaa aaagaaatta aagtgggtta
       61 aaaaagaaat taactgagta aaaactctcg aaattcaatt cgctatatta tcactcaact
      121 tgaacaaata ccagaaccac aaaaaacaga tgtaaaggaa aaatatggac ttaaaatttg
      181 aaggggttga tttagaatac aaaaaagcta aaaacaatct tccagagtct ttctgggaaa
      241 cctactcagc atttgcaaat acaaatggcg ggaaaataat tctcgggata gatgaaaaaa
      301 acattgacac ctaccaaaga gtcaatagac tccctgcgaa acaaaattat gaagcaagta
      361 aacaactaac cgatgcacga tttaagcgtc ttgttggtgt tcaacgcacg acttttgaag
      421 agatgttagc tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac
      481 ctaaattaag cctagaag
//
LOCUS       DAVLXF010000215          498 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVLXF010000215 DAVLXF010000000
VERSION     DAVLXF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.215"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            <1..>498
                     /locus_tag="VYO12_002174"
     CDS             <1..>498
                     /locus_tag="VYO12_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV1588682.1"
                     /translation="SQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKK
                     EYILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRV
                     DHRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTL
                     KGWIG"
BASE COUNT          246 a           57 c           97 g           98 t
ORIGIN      
        1 atcacaagga atgatagtag attatgcaat tcatgatgag agtcaagata aaaatggaaa
       61 tatccattgt catataatga ccataatgcg accaataaac gaaaaaggag agttcttagc
      121 caagtcaaaa aaagaatata tcctagatga aaaaggagaa aaagtattaa acaaaaatgg
      181 aaaaccaaag acaagaaaag tagaactaac aacctggaac gatacaggca atgtagaaaa
      241 atggagagaa aatttttcag atctttgtaa caaatattta gaaagagcag gagcagaaaa
      301 aagagtagac cataggagtt ttaaaagaca aaattcagat tatctaccga caatccattt
      361 aggatcagca gcaagtgcaa tggaaagaaa gggaatagaa actgacaagg gaaactataa
      421 tagagaaata agaaaatata atcaacttgt aaaaacaata aaagaagaga taaaaacatt
      481 aaagggttgg ataggaaa
//
LOCUS       DAVLXF010000216          448 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVLXF010000216 DAVLXF010000000
VERSION     DAVLXF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.216"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            1..448
                     /locus_tag="VYO12_002175"
                     /pseudo
     CDS             1..448
                     /locus_tag="VYO12_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           85 c          102 g          115 t
ORIGIN      
        1 atgtgcgaga ataccgcact tatgaagaaa ttgcggctga ttttggtgtt cacgaaagca
       61 acttactccg tcggagccga tgggttgaag taactcttgt tcaaagtggt gttacgattt
      121 caagaactcc tctcagttct gaggacacgg taatgattga tgcgacggaa gtacaaatca
      181 atcgccctaa aaaaagaatt agcgaatcat tctggtaaaa agaaatttca cgctatgaag
      241 gctcaagcga ttgtcacaag tcaagggaga attgtttctt tggatatcgc tgtgaactat
      301 agtcatgata tgaagttgtt caaaatgagt tgcagaaata tcggacaagc tggtaaaatc
      361 ttggccgaca gtggttatca agggctcatg aagatatatc ctcaagcaca aactccacgt
      421 aaatccagca aactcaagcc gctaacag
//
LOCUS       DAVLXF010000217          443 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVLXF010000217 DAVLXF010000000
VERSION     DAVLXF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.217"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            14..>443
                     /locus_tag="VYO12_002176"
     CDS             14..>443
                     /locus_tag="VYO12_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1588683.1"
                     /translation="MPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFV
                     GNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVRE
                     FLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFNLLSF"
BASE COUNT          160 a           47 c           91 g          145 t
ORIGIN      
        1 tgatgatgaa tatatgccag atcgaatttt aaagttgatg gcttgtttta aaaagagtag
       61 aatgaagaat ttagctttag tttatagtta tggaataata atttatccaa atggtacacg
      121 agaagaggag aagaccgatt ttgttggaaa tcccttgttt gttcaaatgg ttcacaatat
      181 agcaggtacg tcattttggt tgtgtaaaaa agaggtgcta gaattaatta atggttttga
      241 gaaaatagat tcacatcagg acggtgttgt tttattaaaa ctacttgctc aaggatacca
      301 aattgatata gtgcgagaat tcttggtgaa ttactacgct cacagtaaag aaaacggtat
      361 cactggagtg acacaaaaaa caattaatgc agatgaagaa tattataatt actgtaggaa
      421 atattttaat ttattgagtt tca
//
LOCUS       DAVLXF010000218          355 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVLXF010000218 DAVLXF010000000
VERSION     DAVLXF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.218"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..291)
                     /locus_tag="VYO12_002177"
     CDS             complement(<1..291)
                     /locus_tag="VYO12_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEV1588684.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYII"
BASE COUNT          121 a           50 c           54 g          130 t
ORIGIN      
        1 aatgatatat ataggtaatg gtatgtaggg taggaagaac aaactgaata ataagattaa
       61 aattggtaaa ttagatatta atgctgctcc taaatttttc caataatctt taaaaaaact
      121 gtttgcgaag tctctatagg gataggattc ctcgtgtcgt acaattttta ttagagttgc
      181 tattagggca ctttcacctg atccgatagc aataactgtt actactgacg ctacatagag
      241 tagatttaac attataagat ttgaaacttt atcaataaat tttatctgca tctttaactc
      301 ctgctgtttt tatacttata atatcacatc atttgagttt catcaacttt gaata
//
LOCUS       DAVLXF010000219          306 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVLXF010000219 DAVLXF010000000
VERSION     DAVLXF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.219"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            48..306
                     /locus_tag="VYO12_002178"
                     /pseudo
     CDS             48..306
                     /locus_tag="VYO12_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          112 a           51 c           57 g           86 t
ORIGIN      
        1 ataaacaatt ctaatattag gtacatgatt aaattgattg atcgttaatg ttggaacagg
       61 cattcaaaga gaagcactac gagaatacga ttctccatag tgaccaaggc tggcaatatc
      121 aacacgattc ttatcatcgg ttcctagaga gtaagggaat tcaagcatct atgtcacgca
      181 agggaaacag ccaagacaac ggtatgatgg aatctttctt tggcatttta aaatccgaaa
      241 tgttttatgg ctatgagaaa acatttaaat cacttaatca attggaacaa gccattatag
      301 actata
//
LOCUS       DAVLXF010000220          294 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065623
            SAMN33607168-rid17945223.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVLXF010000220 DAVLXF010000000
VERSION     DAVLXF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607168
            Sequence Read Archive: SRR23725546
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721423.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721423.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:00:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,179
            CDSs (total)                      :: 2,132
            Genes (coding)                    :: 2,007
            CDSs (with protein)               :: 2,007
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 58 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 38 of 125
            Pseudo Genes (multiple problems)  :: 28 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607168-rid17945223.denovo.221"
                     /strain="AUSMDU00065623"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST433"
     gene            complement(<1..243)
                     /locus_tag="VYO12_002179"
     CDS             complement(<1..243)
                     /locus_tag="VYO12_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1588685.1"
                     /translation="MSSQMKAFLNELQGNMEVVGEFFDNPEKVIRKFGIVGREKEALL
                     IRDLNDMDDYALSIQNSVASPSGAHSSTCHFVRQDLQ"
BASE COUNT           91 a           63 c           35 g          105 t
ORIGIN      
        1 ttgtaaatct tgtctaacaa aatggcaagt gctactatgt gccccagaag gcgatgcaac
       61 gctattttga attgaaagag cataatcatc catatcattt aagtcacgga ttagcaatgc
      121 ttccttctct cttccgacaa ttccaaattt tctaattacc ttttcaggat tatcaaaaaa
      181 ttctccaaca acttccatat ttccttgaag ttcattcaag aaagctttca tttgactact
      241 cattatatag ctccttttct attactttat ttggaatcaa aacttacttg taca
//
